LOCUS       DARDHA010000001        60930 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000001 DARDHA010000000
VERSION     DARDHA010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.001"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            17..535
                     /locus_tag="SDA10_000001"
     CDS             17..535
                     /locus_tag="SDA10_000001"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG4238938.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            663..1346
                     /locus_tag="SDA10_000002"
     CDS             663..1346
                     /locus_tag="SDA10_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEG4238939.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1655..1728
                     /locus_tag="SDA10_000003"
     tRNA            1655..1728
                     /locus_tag="SDA10_000003"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1689..1691,aa:Arg,seq:tct)
     gene            1887..2246
                     /locus_tag="SDA10_000004"
     CDS             1887..2246
                     /locus_tag="SDA10_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEG4238940.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2369..4000
                     /locus_tag="SDA10_000005"
     CDS             2369..4000
                     /locus_tag="SDA10_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEG4238941.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4062..4703)
                     /locus_tag="SDA10_000006"
     CDS             complement(4062..4703)
                     /locus_tag="SDA10_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEG4238942.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    4889..4965
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            4985..5293
                     /gene="rplU"
                     /locus_tag="SDA10_000007"
     CDS             4985..5293
                     /gene="rplU"
                     /locus_tag="SDA10_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEG4238943.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5320..5664
                     /locus_tag="SDA10_000008"
     CDS             5320..5664
                     /locus_tag="SDA10_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEG4238944.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5677..5970
                     /gene="rpmA"
                     /locus_tag="SDA10_000009"
     CDS             5677..5970
                     /gene="rpmA"
                     /locus_tag="SDA10_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEG4238945.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6404..9211
                     /locus_tag="SDA10_000010"
     CDS             6404..9211
                     /locus_tag="SDA10_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG4238946.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9402..9893
                     /locus_tag="SDA10_000011"
     CDS             9402..9893
                     /locus_tag="SDA10_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4238947.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9996..10985
                     /gene="manX"
                     /locus_tag="SDA10_000012"
     CDS             9996..10985
                     /gene="manX"
                     /locus_tag="SDA10_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEG4238948.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11018..11821
                     /locus_tag="SDA10_000013"
     CDS             11018..11821
                     /locus_tag="SDA10_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEG4238949.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11842..12756
                     /locus_tag="SDA10_000014"
     CDS             11842..12756
                     /locus_tag="SDA10_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEG4238950.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12839..13213
                     /locus_tag="SDA10_000015"
     CDS             12839..13213
                     /locus_tag="SDA10_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEG4238951.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13352..13597
                     /locus_tag="SDA10_000016"
     CDS             13352..13597
                     /locus_tag="SDA10_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG4238952.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13769..14833
                     /locus_tag="SDA10_000017"
     CDS             13769..14833
                     /locus_tag="SDA10_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEG4238953.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15079..15504
                     /locus_tag="SDA10_000018"
     CDS             15079..15504
                     /locus_tag="SDA10_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG4238954.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15497..15946
                     /gene="nusB"
                     /locus_tag="SDA10_000019"
     CDS             15497..15946
                     /gene="nusB"
                     /locus_tag="SDA10_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEG4238955.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16043..16897
                     /locus_tag="SDA10_000020"
     CDS             16043..16897
                     /locus_tag="SDA10_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEG4238956.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16898..18235
                     /locus_tag="SDA10_000021"
     CDS             16898..18235
                     /locus_tag="SDA10_000021"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEG4238957.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18240..18464
                     /locus_tag="SDA10_000022"
     CDS             18240..18464
                     /locus_tag="SDA10_000022"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEG4238958.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18466..19356
                     /locus_tag="SDA10_000023"
     CDS             18466..19356
                     /locus_tag="SDA10_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEG4238959.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19365..20183
                     /locus_tag="SDA10_000024"
     CDS             19365..20183
                     /locus_tag="SDA10_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEG4238960.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20290..20739
                     /locus_tag="SDA10_000025"
     CDS             20290..20739
                     /locus_tag="SDA10_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEG4238961.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20757..22439
                     /gene="recN"
                     /locus_tag="SDA10_000026"
     CDS             20757..22439
                     /gene="recN"
                     /locus_tag="SDA10_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEG4238962.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22494..22610)
                     /locus_tag="SDA10_000027"
     CDS             complement(22494..22610)
                     /locus_tag="SDA10_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEG4238963.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22683..23639)
                     /locus_tag="SDA10_000028"
     CDS             complement(22683..23639)
                     /locus_tag="SDA10_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG4238964.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23810..24178
                     /locus_tag="SDA10_000029"
     CDS             23810..24178
                     /locus_tag="SDA10_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEG4238965.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24348..24779
                     /gene="mraZ"
                     /locus_tag="SDA10_000030"
     CDS             24348..24779
                     /gene="mraZ"
                     /locus_tag="SDA10_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEG4238966.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24800..25759
                     /gene="rsmH"
                     /locus_tag="SDA10_000031"
     CDS             24800..25759
                     /gene="rsmH"
                     /locus_tag="SDA10_000031"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEG4238967.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25764..26165
                     /gene="ftsL"
                     /locus_tag="SDA10_000032"
     CDS             25764..26165
                     /gene="ftsL"
                     /locus_tag="SDA10_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEG4238968.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26166..28358
                     /locus_tag="SDA10_000033"
     CDS             26166..28358
                     /locus_tag="SDA10_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEG4238969.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28384..29346
                     /locus_tag="SDA10_000034"
     CDS             28384..29346
                     /locus_tag="SDA10_000034"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEG4238970.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29360..30721
                     /locus_tag="SDA10_000035"
     CDS             29360..30721
                     /locus_tag="SDA10_000035"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEG4238971.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30747..31835
                     /gene="murG"
                     /locus_tag="SDA10_000036"
     CDS             30747..31835
                     /gene="murG"
                     /locus_tag="SDA10_000036"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEG4238972.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31850..33070
                     /locus_tag="SDA10_000037"
     CDS             31850..33070
                     /locus_tag="SDA10_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEG4238973.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33226..34551
                     /gene="ftsA"
                     /locus_tag="SDA10_000038"
     CDS             33226..34551
                     /gene="ftsA"
                     /locus_tag="SDA10_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEG4238974.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34574..35815
                     /gene="ftsZ"
                     /locus_tag="SDA10_000039"
     CDS             34574..35815
                     /gene="ftsZ"
                     /locus_tag="SDA10_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEG4238975.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35832..36509
                     /locus_tag="SDA10_000040"
     CDS             35832..36509
                     /locus_tag="SDA10_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEG4238976.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36523..37122
                     /locus_tag="SDA10_000041"
     CDS             36523..37122
                     /locus_tag="SDA10_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEG4238977.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37137..37424
                     /locus_tag="SDA10_000042"
     CDS             37137..37424
                     /locus_tag="SDA10_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEG4238978.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37473..38255
                     /locus_tag="SDA10_000043"
     CDS             37473..38255
                     /locus_tag="SDA10_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEG4238979.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38276..38983
                     /locus_tag="SDA10_000044"
     CDS             38276..38983
                     /locus_tag="SDA10_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEG4238980.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39245..42043
                     /gene="ileS"
                     /locus_tag="SDA10_000045"
     CDS             39245..42043
                     /gene="ileS"
                     /locus_tag="SDA10_000045"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEG4238981.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42092..43618)
                     /locus_tag="SDA10_000046"
     CDS             complement(42092..43618)
                     /locus_tag="SDA10_000046"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEG4238982.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43633..44280)
                     /locus_tag="SDA10_000047"
     CDS             complement(43633..44280)
                     /locus_tag="SDA10_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEG4238983.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44464..44793)
                     /locus_tag="SDA10_000048"
     CDS             complement(44464..44793)
                     /locus_tag="SDA10_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HEG4238984.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44970..45698
                     /gene="sfsA"
                     /locus_tag="SDA10_000049"
     CDS             44970..45698
                     /gene="sfsA"
                     /locus_tag="SDA10_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEG4238985.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45714..46727
                     /locus_tag="SDA10_000050"
     CDS             45714..46727
                     /locus_tag="SDA10_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG4238986.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46727..48004
                     /locus_tag="SDA10_000051"
     CDS             46727..48004
                     /locus_tag="SDA10_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG4238987.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48067..50769
                     /locus_tag="SDA10_000052"
     CDS             48067..50769
                     /locus_tag="SDA10_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG4238988.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50921..51238
                     /locus_tag="SDA10_000053"
     CDS             50921..51238
                     /locus_tag="SDA10_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4238989.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51268..51588
                     /locus_tag="SDA10_000054"
     CDS             51268..51588
                     /locus_tag="SDA10_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG4238990.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51696..53156
                     /locus_tag="SDA10_000055"
     CDS             51696..53156
                     /locus_tag="SDA10_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4238991.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53224..53445
                     /locus_tag="SDA10_000056"
     CDS             53224..53445
                     /locus_tag="SDA10_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4238992.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53476..53658
                     /locus_tag="SDA10_000057"
     CDS             53476..53658
                     /locus_tag="SDA10_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HEG4238993.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53658..54071
                     /locus_tag="SDA10_000058"
     CDS             53658..54071
                     /locus_tag="SDA10_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEG4238994.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54194..55375
                     /locus_tag="SDA10_000059"
     CDS             54194..55375
                     /locus_tag="SDA10_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4238995.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55446..55610)
                     /locus_tag="SDA10_000060"
     CDS             complement(55446..55610)
                     /locus_tag="SDA10_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4238996.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55764..55837
                     /locus_tag="SDA10_000061"
     tRNA            55764..55837
                     /locus_tag="SDA10_000061"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55798..55800,aa:Arg,seq:cct)
     gene            complement(55874..56074)
                     /locus_tag="SDA10_000062"
     CDS             complement(55874..56074)
                     /locus_tag="SDA10_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG4238997.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(56379..57611)
                     /locus_tag="SDA10_000063"
     CDS             complement(56379..57611)
                     /locus_tag="SDA10_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEG4238998.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            57865..58434
                     /locus_tag="SDA10_000064"
     CDS             57865..58434
                     /locus_tag="SDA10_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4238999.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(58612..59052)
                     /locus_tag="SDA10_000065"
     CDS             complement(58612..59052)
                     /locus_tag="SDA10_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEG4239000.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            59210..59974
                     /gene="map"
                     /locus_tag="SDA10_000066"
     CDS             59210..59974
                     /gene="map"
                     /locus_tag="SDA10_000066"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEG4239001.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            60006..60929
                     /locus_tag="SDA10_000067"
     CDS             60006..60929
                     /locus_tag="SDA10_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEG4239002.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
BASE COUNT        20004 a        10372 c        13049 g        17505 t
ORIGIN      
        1 aaagaagttg aaaacgatac ctgattacaa aaaaataact tttataagtt ctataacgtt
       61 gattatattt ttaattctta ttcttctcgt cgtcttggtt ccccgaattt ttggtttgac
      121 tccttatatt attgaggaca aagaaatgag tccatattat gcacaaggaa atctgatata
      181 tgtacgaccg aaaaatacaa atgagatttt agtcggagat attattacat attatgaaaa
      241 ttcaggaaaa aaaatctcga cgcgtcgcgt aatagccgtt gaaaacaacc acgaagattt
      301 ttatacaaaa gctgatacaa agacttatat tgagcctgga gtagtaaaaa agagaaatat
      361 tatcggtagt cccatctttc agattccata tattggacta gtcctttcaa aaacggcatt
      421 tgcatggatt caggggttat tctttacgat tgctttttgc ctaacaggaa tcacagcatg
      481 gaataccttt gtagagttga aaaagaagcg gagacgaaca cagaaatttt tgtaagaatg
      541 ttaaaaacat aaatgtattc cagagatcca acgactcttc tgaacatgaa acacgagaaa
      601 acactcatgc agcttgtaga tttagtcagt tgttcgtata atgtgtgaag aggaggagta
      661 gtatgcgcaa tgaaatgatg catcgtcctg tagtcaaaga agaactattg gattttatgc
      721 gtacccagca aaaaaaatta ccgggtgagc taggcaaact ggaagaagaa gctcatgtag
      781 cagaagtacc agttattccc catgaaacag ttgtattttt gaaattttta cttggacagc
      841 ttcagcctga acgaatcttg gagatcggag cagctatcgg tttttcatca agtgtcatgg
      901 ccacgacaat cggagaaaac gctcatgtta cgacaattga tcgtttcgat gtcatgatcg
      961 agaaagctaa aaaaaactat gaacgtctag gactgacaga taaagtgact ttgcttgaag
     1021 gtcaagcagc agatatcttg ccggagttgt ctggtccgta tgactttatt tttatggata
     1081 gtgcaaaatc aaaatatatt gaatttttac ctgaatgctt acgtttgtta agaaaaggtg
     1141 gcgttttgat ggtagatgac atcttccaag gaggaacgat cttgctccca gatgaagaga
     1201 ttccccgtgg caaacgagct atccatcgta aattaaatga atttcttcgt gttgtgatgg
     1261 atcatcccga tttgacatcg acaatcttgc cattaggtga tggtgtgatc ttaatgacaa
     1321 aggaatcaga aacaatcgag ttgtgataca tgattatggc agcctgtaga taatcgattg
     1381 aatcgagggc tcaattcgaa cttgatgtaa aagtttctat atttgtaagt caattaaagg
     1441 atacaggaaa tctaaataaa caggagtagc atgaagtcaa tgtagctcct gttttcaatt
     1501 gataacctta gaacagctga aggagaatgt ctcgttttaa aagattgatt tagtagttgg
     1561 gccaaaataa ttcattattt gctaattaaa taactatttc gtattgacag aagcaattta
     1621 attatctata attattgatg ttccgctgat tcttgtcata gtagctcagc tggatagagc
     1681 atccgccttc taagcggacg gtcgggggtt cgaatccctc ctgtgacgct aaaaaaccgt
     1741 tagaaactga ggtttctaac ggtttttctt tgttttttgt gccaatattg tttgttttca
     1801 aaggcaaata aaataaaaaa acatccttat cgagtacaat tataaggagc aaaaaaataa
     1861 aactcatcag aaaagaggaa gaagatatga cgatactatc tagaatttta gtgactctag
     1921 ttgcaattga atttttttat atcatgtata tcgaaacggt gagaactgat tctgacacga
     1981 caagtagggt ttttaaaatg agtaaagaag agttaagcag aaaatctgtt caaacattgt
     2041 ttaagaacca aggagtgtac aatggactat taggagtggg actactttat ggagcttatc
     2101 tttcttccgc atctaaagaa atcacaagta tgctattaat atctattttt tttgttgcat
     2161 tatatggcag tttgactagc gatcgttgga ttttactcaa acaaggtggg ctagctctgt
     2221 tggcattgct ttctattttc ttataaatat agaaaaaata agaatttatc taacaggaaa
     2281 ctttgtgaaa aatattactt tatctcatga aatatgatat tctaatgaat ggtattttag
     2341 aaaatttcat gaaaaaggag ttagataggt gttcaaaaat tatgtttcct cactaaaaaa
     2401 agaattttca ggttatgatc tttcaagatt tcagaaagat cttttggctg ggatcacagt
     2461 agctgctgta gcattgcctt tagccttggc ttttggggta tcaagcggag ctgatgcggc
     2521 cgcaggtttg attacggcta tcattgccgg tatcgttatt ggcggtttat caggcggttt
     2581 ttatcaaata tcaggtccga caggtgcgat ggcagctatc ttgatgtcta ttgcggcaac
     2641 aaagggaatg caaggtgtat tgcttgctac ttttttagcg ggtctatttt tacttgcagc
     2701 aggacttttc cgattaggaa cactgacttc ttttatccca gcacccgtaa ttacggggtt
     2761 tacttcaggt attgctatca ttattgcatt aggtcagatc gataatttat ttggtgttca
     2821 ctcggaaggt acaaacgtta ttgaaaaact aagtagttat caaacgatag gatttccgat
     2881 aaacacagcc tctttgatca tgggagggct agtcatttta gggatgttta tctatccaaa
     2941 aaaatgggcg aaacgaatgc ctagttcgct gctggctatc attttggcaa cagcagttat
     3001 gctattgttc catttaccag tagcaactgt gggtaagatt ccacaaacat tgatcagtag
     3061 taatcgtttg aatatgggag attttagctt ttctgccttg caacaagtgg cagttccagc
     3121 tattagcatt gctttattag gaatgattga aagcttgcta tgtggtgctt cggctggccg
     3181 aatggcaaat aagcccttag atagcaatca agaattaatg gcgcaaggaa ttggaaatct
     3241 gttgcttcct ttctttggcg ggattcctgc tactgcggcc atcgcccgta caagcgtggc
     3301 catcaaatct ggtgcacaaa ctagattagc tggtatgata catgcagttg tcttactgct
     3361 ctccatgctt gtttttgcgc cagtgatgtc acagattcca atgccagcac ttgcaggtgt
     3421 tttgattgtg acggcttggc gtatgaatga atgggaaacg atcaaggagc tatttactaa
     3481 taaatattgg tcagcagttc tattgtttat ccttacgatg atttgtacag ttatctttga
     3541 tttaagtatt gctatcgtga tcggggtgat cggcggctgt gtattttttg ttgtcaaaag
     3601 cgctgctatc acgatttcat tagaaacgat cgattggcaa agaatggggc tgccagaaac
     3661 ggaaaagctg aataactgga cagtagtgta tattagtggt ccactgtttt ttatgtcttc
     3721 agaaaaactc aaaaataaat tggaagaact aaatcatcac gatggtgtca ttttctccat
     3781 gcgaggcgta ccaagtattg atttgacggc acgtgcagtt tttgatgaat ttcaagaaaa
     3841 agcagagcaa agaggacaaa cgattatcta tacttccttg cagccagaag tagaaaaaca
     3901 actttctcac ttgtgggaac atacattcac gcatcaacat caaacagttg ctcatgcgtt
     3961 gacagcttta catgcgaaat atactgcaat tagccattaa aacaatgaaa aagaacagac
     4021 aagactgtaa actgaagatt ttatggtctt gtctgttctt tttaatattg ctgtaaaagt
     4081 gaagctagta cttgccagtc ttgatggata acaggtacaa gggagcgatt gtagaaaata
     4141 gctgctggat gaaaaagtgg aagaattcga tagttttttt ctgaccattc gtaggcatcc
     4201 ttttgttctg ttaattgtaa gacaggccct gtatagagtt gaccatgatt tttggtaacc
     4261 gtatattctt tgccaagtaa acgttgaaga ccaatgtttc caagtgtcgc aattaatttt
     4321 ggctggacct tcatcagttc gtaatccaat attggtgcat gcgcaaatac ttcggaacga
     4381 gtaggtgtcc gatttggata aacagtttct gactgttgtg tccgtttatt gatccgatga
     4441 gtcacgcgat acgggcggct ccttacagca ctggtgatat acacctcttc tcgtgttaag
     4501 ccagttgaag aaagtgcctg catcaattct tttccagctt gaccgctaaa aggaacatgc
     4561 gtatcaatct ctgttttccc tggggcttct cccacgagca tcagtttagg atgaagaggt
     4621 ccttgcccag cgataaatcc ttctacagga cggccgttca ttcttaaaga agcttcttgt
     4681 atcagttctt ttgggtattc catttatgct cctccttttt cttttattat aagaataaaa
     4741 gaaacacaat gcgactattt tatttggtag aactgtgagg atagaaaagc agaagtattg
     4801 cttgaatttt tgttttactt tttgatttta aatttagttg actaacggtt gctgttctgg
     4861 taagctagta ctgttgtaat tgtggactcc acagctacaa ccgctcagaa caagtaataa
     4921 gcacagcata tgctgtcact tcggatggcg agtctaagta tatcagaagg aggtgcttta
     4981 tagcatgtac gcaattatta aaactggtgg taaacaagta aaagttgaag tgggacaagc
     5041 aatctatgtt gaaaaacttg acgttgaagc aggagaaaaa gttgtttttg acgaagttat
     5101 cttagtaggc ggagaatcta caaaagtagg cgctccaact gttgcaggtg caactgttga
     5161 aggtactgtt gaaaaacacg gcaaacaaaa gaaagtcgtt actttcaaat acaaacctaa
     5221 aaaacattca caccgtaaac aaggtcatcg tcaaccatat acaaaagtgg ttatcgacgc
     5281 gatcaacgct taatagcgtt tgagaaagaa ggcctatata tgatcaaagg aacatttaag
     5341 cgaaatgacg caggtcagat cgtttcattc actttgaccg ggcatgctga tgctggtcct
     5401 tatggaagtg atatcgtttg tgcgggtgtt tctgcgttag cgatcagcac tgtcaacggg
     5461 attgcctcat tagccggttt tgagccgatt gttgaaatga atgaagaaga aggcggttat
     5521 ctttataccg aagtcacttc aggaatgaca caagaacaaa acaatattgc tgagattcta
     5581 ttagaaaatc ttttattagg attacaatcg atagaagctg aaaactcaga atatatccaa
     5641 attaaaacaa ttaatgataa ataggaggtg cagaccatgt tgttatctat gaatctacaa
     5701 ttatttgccc acaaaaaagg tggcggttct acatctaacg gccgtgactc agaatcaaaa
     5761 cgtctaggtg ctaaacgtgc tgatggacaa acagttacag gtggatcaat tctttaccgt
     5821 caacgcggta caaaaatcta tccaggtgta aacgtgggta tcggtggaga cgacacttta
     5881 tttgcaaaag ttgatggcgt agtacgtttc gaacgtaaag gccgtaacaa aaaacaagtg
     5941 tctgtttatc cagtagctca agaagcttaa ttgaatctta tgattaaaga ggttgtgaca
     6001 agacttttgt cacagccttt tttgtttata aaaataaaag ttgtagtgga ggctacgaag
     6061 tttactttgt ctttttacac ttaatatttt tttgccaatt tggaatcagg atgttttgat
     6121 aactatcgtt ttcaattgat ggaagctata ttgtttgcta tcagtgaaat aattatatgg
     6181 taaacagtgt tctttttctt gtttccatta taaaaatcgt atcgggattt ttcgttcttt
     6241 taaattgaac atttctatct atccgcgtgt tatcaacaaa acaaaatcag gtaaatagtt
     6301 gacgaaacag aaataaatac atagtattat gttttgaagc ctaagtacaa tagtctatag
     6361 aaaaggagcg ataccattgg caaaacgcat tgaccgaatt tatatgtatg tcaaagaaaa
     6421 aaccgctcat ttaacaaagg cagaatatga tcaaggagta acaacacagg aaattgctga
     6481 agtgttaggg attcagcgaa caaatagcag taaagatctg aatcagctag tgcgcgaagg
     6541 aaaactgata aagacagacg gacgaccagt acgttacata tatcaaacac tcgttacgca
     6601 cgtaaagcca gttacaaaac atgtagtcag ttacaaagaa acgccggtct ctgaagaaaa
     6661 aaatacactc caaatagata ctaaggatat ctttgcaaaa atcattggag ctaacggcag
     6721 catgaaaaat tcagtggaac aggcaaaagc tgcgattttg tatccgccaa gaggattaaa
     6781 ttgtttgatt acaggaccta ccggatcagg aaagacttat tttgcacacg cgatgtatca
     6841 ttttgcaaaa gcgcatcatg taatagatga ggaaacggaa ctgattgttt tcaattgtgc
     6901 tgattatgcc aataaccctg agcttttgat gagtcattta tttgggtacg caaaaggtgc
     6961 ttttacaggc gcagatgaag aaaaaatggg gattatcgat caagcggatg gcggtatgct
     7021 gtttttggat gagattcacc ggttgccgcc tgaaggtcaa gaaatgatct tttactttat
     7081 ggatcatgga acttacagtc gtcttgggga aacaacgaaa tcccatgaag caaatgtacg
     7141 gatcgtaggt gcgaccacag aagacccagg ttcttctcta ttagaaactt ttgttcggcg
     7201 tatcccaatc aatatcaaac ttccggcttt tgaaaaacgt cctgcaaatg agaaagtcga
     7261 tttagtaaaa atcatgattg cccacgaagc aaaccgcacg cagcgaaaga tttctttgac
     7321 agaagatgta gcaaaagcat tgatcggcag tgtgacctat ggaaatatag gacaattgaa
     7381 gtcaaatatc cagttagtat gtgcacgagg atttctaaat catatgcaat cacccgaaat
     7441 ctcgatcaca atcgatgatt tgacagaagg gattcgtagc ggattgattc aattagcaag
     7501 caaccgcagt gcaatggcag agctttctaa aatattggag ccaaagataa ctgtagcacc
     7561 aaatgatacg atcatgaaaa tccaatcgga ctcctatgag ttaccttata atctatatga
     7621 tatcatcgga gataaagcag ctttactgaa gtcggatggt ttggatcaag aagcaatcaa
     7681 tcatttcatt tctacagata tcaacatcca tctgaagtct ttctataaag accatggttt
     7741 ttcttttaat gcagacagca aattagcaga atttgtagat ccgaaaatca tagaagtgac
     7801 caatcagatt tatgtaatgg tcaaaagatc tttaccatat gagtttcagc aaaactttat
     7861 ttatgctatg agcttgcata tcagttcttt tttgaaaaaa atcaacttag gagaagaacg
     7921 gcatacgaat gataacatcc gtgagatggc ttttgatttt ccagatgaat atgctgttgc
     7981 gaaagaagtg cgtagttata tcgaaacgta ttttcaagtg gagattccag aaagtgaaga
     8041 ctattacttg accgttctac tcgtctcgtt acgggcaaat caagcaagcg gcagaatcgg
     8101 tgtagtcgtt gcagctcacg gaaacagcac agcaagcagt atggtccaag ttgtccaaca
     8161 gttgttagat gcagagaacg tacgagcagt agacatgcct ttggatatgg atccgaaaac
     8221 agcattggac cgaattgaac gcaatgtaca agaagtagat gaaggaagcg gggtcatact
     8281 cttagtagat atggggtccc ttgcgtcgtt taattcgcaa attcaaagag acactggaat
     8341 tccagtacgt actgttgata tggtaacaac ttctctagtt ttggaaactg tacgaaaggt
     8401 ctcagtatta gggacagatc tagatatgtt gcatgaatca ttgaaaaatt ttcgaggtta
     8461 cgcagaaata tcttctgtag aatctgttca ggaagcaaaa cctattcata agaaaaaagc
     8521 tgttttggct atctgcgcat ctggcgaagg tacagcacaa agaatcaaag aattgatcga
     8581 acgagcggta aacaagcgtc aagaaacaga attagaagtt ctagctttgt cgattgtgga
     8641 agtgaaagaa gaactgccag ttatccaaaa agaatatcag atcatagcta ctactgggat
     8701 tactgatttg aatatccaag ccccatttat tcctttagaa cgttttattg atcaaaatat
     8761 cgaagtcatt ttggatcagt tgctgatgga atcagaattg gaggaaactg aattcatctc
     8821 cctagatgaa gagtcagcta aaaatacgtg tgttgagttt atctctgata acttcatctt
     8881 tattaatggt agtaagttaa tcgatccaat gtggcaattt tctacacaaa tctctcagac
     8941 gacgggaatc ggcgatgaag aatatggatt caaaatcaac ctagtgatgc acacagcagg
     9001 aatgatcgaa cgtattattc ggaatgaacc attgactgtg gaagaaaacg aattgacaaa
     9061 tacaacgaat gatcctcttt atagccaatt ggcagcctca gttgttctgt tggaagatca
     9121 gataaaagta aaagtgccga tagaagaaat gtattatctg ctacgtctag tccataatca
     9181 gttagataaa aaagaataca ctgtgccatg aatacacaaa aacgatacac ttttatagtg
     9241 tatcgttttt gtgtattggt taagaatcct ttaaattcaa ttagtgtatc gtaaaaaagt
     9301 tggcacgctt tttgcttata gtagtgtgtg ctaggaggtg gttgttcaca cagaaatgat
     9361 aggggtttct gtgaagtagt gaggaagaag gagttttgca gatggatatt cgattggcac
     9421 gtatagatga tcgtttaatc cacgggcaag tcgcaacagt ttggtcgaag caaacaggtg
     9481 ttgaacgtat cattgtgatc agcgatgtag tcgctcaaga taaattgaga aagtttctac
     9541 taaaagaagc tgcaccgccg gggatcacag caaatgtaat aaccgtgcag aaaatgattc
     9601 aaatctatca tagtggatta ttatcacgag caaaagtgat gcttttattc accaatcccc
     9661 aagatgttga agcggttgtc cgctgcggtg tacagttgaa gtcgttgaat atcggcggta
     9721 tgagttttac tgaaggcaaa tcaatgatta ccaattttgt ttcagtaaat caacaagata
     9781 ttgattcctt tcgttacttg aacagtcaag aagtggagtt agagattcga aaagtacctg
     9841 ctgacaggaa aatccttttg atggaactac tagcgaaaat caaaaaggtg tagcaagaat
     9901 ggttgttgga aaaggtcaaa taattctgtt gtaatggtta gcaattatta atatttttga
     9961 atttgttaca acaaaagtaa ataggaggta taaatatggt agggattatc ctagcaagtc
    10021 atggacaatt tgctgaagga atcttacaat ccggttcaat gatctttggc gaacaagaaa
    10081 acgtcaaagc cgtgattttg aaaccaagtg aaggtccaga tgacttgaga gccaaattgg
    10141 aagaagcggt tgcttctttt gataatcaag acgaagtgtt attcttagtt gatttatggg
    10201 gcggaacacc gttcaatcaa tcaaacacat tattcgaaga gcacaaagat aaatgggcaa
    10261 tcgtcagcgg tttgaattta ccaatgttga ttgaagccta cgcatcacgc ttttcaatgg
    10321 aatctgctca tgaaattgca gcacacatca ttgaaacagc aaaagacggt gtaaaagtaa
    10381 aaccggaaga attagaaccg gcagaagcac cgaaagcagc agttgaagac gcacaaccta
    10441 aaggagcact tccagaagga actgtggtcg gagacggaaa aatcaaatat gtattagcac
    10501 gtgttgactc acgtctgtta catggacaag ttgcgacagc ttggacaaaa gcagtgcaac
    10561 caaaccgtat tatcgttgtt tcagatgcag tatcaaagga tgatctgcgt aagagactaa
    10621 tcgaacaagc tgctcctcca ggtgtaaaag caaatgttat tcctattagc aaaatgatcg
    10681 aagtagcaaa agaccctcgt tttggaaata ccaaagcatt gcttttattt gaaaatcctg
    10741 aagacgtatt gacagctgtt gaaggtggcg tagacatcaa agaattaaat gttgggtcaa
    10801 tggcgcattc ggtcggtaaa gtcgttgtaa gtaaagtatt gtctatggga caagaagacg
    10861 ttgaagcgtt tgagaagctt gaacaaaaag gtgtgaaatt cgatgtacgt aaagtaccta
    10921 atgattcacg cgataacatg gatgatattt tgaaaaaagc aaaagctgaa ttagcaaaag
    10981 cgtaataaat aatattaaaa tttataggag gcttatcatg tctattattt caataatttt
    11041 agtcgtatta gttgcctttc tagctggtat ggaaggtatt cttgatgaat ttcaatttca
    11101 tcaaccacta gtggcatgta cattgatcgg tttagttacc ggtaacctcg aagctggtat
    11161 cgtacttggc ggtacgcttc agatgatcgc tttaggttgg gcaaatatcg gtgccgctgt
    11221 agcaccagat gctgcattag catcagtagc atcagcaatt attttagttt taggtggaca
    11281 aggtgttaaa ggtgttcctt ctgcgatcgc gattgccgtt cctttggctg ttgcagggtt
    11341 gttcttaaca atgatcgtac gtacaatcgc tgttccaatc gttcatttga tggatgcggc
    11401 tgctgaagaa ggaaacatcc gtaaggttga aatgtggcac atcattgctg tatgtttaca
    11461 aggggttcgt atcgcggtcc cagcagcagc gttgctattt atcccagcac aaactgtgca
    11521 atcattcctt gaatcaatgc cagcatggtt aacagatggt atggctatcg gtgggggaat
    11581 ggtcgtagct gtaggttacg ctttggtaat caacatgatg gctacaaaag aagtatggcc
    11641 attcttcgtt attggtttcg ttgtagctgc aatttctcaa ttaacattga ttgctctagg
    11701 tgctttaggt gttgcattag ctcttattta cttgaacctt tctaaaatgg gtggttcatc
    11761 aaataatgga ggcggaagca actctggtga ccctttaggc gacattttga atgactatta
    11821 atcttgaagg aggaaaataa aatggcagaa gaaaaaatca aattatcaaa aaaagatcgt
    11881 ttagctgttg cgtggcgctc taccttcatt caaggttcat ggaactacga acgtatgcaa
    11941 aacggtggct gggcattttc tatgattcca gcaatcaaaa aattatacaa aacaaaagaa
    12001 gaacgttctg cagcattaaa acgtcacttg gaattcttta atacacaccc atacattgct
    12061 tctccaattt taggggttac attggctctg gaagaagaac gtgcgaatgg cgcgcctgtt
    12121 gatgatgtag cgattcaagg ggttaaagta ggtatgatgg gtcctttagc tggtgttggt
    12181 gacccagtgt tctggtttac agtacgtcca atgctaggtg cactaggtgc ttcattagct
    12241 atgggaggaa atatccttgg accaatcatc ttcttcttag gttggaacat tattcgttgg
    12301 gcattcatgt ggtatacaca agaatttggc tacaaagctg gttctaaaat tactgatgac
    12361 ctttctggcg gattgttaca agacgtaaca aaaggtgctt caattttagg gatgttcgta
    12421 ttagctgccc ttgtacaaag atgggtatca atcaaattct taccagttgt atcaactgta
    12481 aaattagata aaggtgctta catcgaatgg gataaattgc cttcaggtgg tgaaggaatc
    12541 aagagtgcat ttgaacaagt taataatggc ttagcccttt ctcctgaaaa ggtaacaaca
    12601 ttacaagaca acttagacca attgattcct ggtttagctg cattaggatt aacattgtta
    12661 tgtatgtggt tgcttaagaa aaaggtttct ccaatcgtta tcatccttgg tttattcgta
    12721 gtcggtgttg ttggacacgt catcggactt ttataatcca taaaaatata gctcgggcgt
    12781 tgtcccgagc tttttttata tagtatactg ttcataaaca aatatctgga gggagaaaat
    12841 ggttcaatca atcaatacaa aagtagactt ggtaattgat gctacttcgt ttacgggtct
    12901 gacagattac gggcaaatca tgataggtga taatggtttt gagttttacc atgcgcgaga
    12961 tagacgcaaa tatatccaaa ttccatggga agaagtggat tatgtactag catcagtcat
    13021 gctaaaggga aaatggattc ctcgatatgc cattcaaacg aaaaaaaatg gtacatacac
    13081 attttcttcc aaagaaccta aaaaagtgct tcgtgcgatc agagaatatg tagatgaaga
    13141 tagaatggta caatcattga gtttctttga tgtagtgaaa cgtggtattc gtagtatgtt
    13201 taaacgcaag taaaaaataa agaaaacttc taagaggagg ttttctttat ttttttactt
    13261 tgcagaagaa aatatgaaat agcaaaacca attatttgtc ccctttaaat cccatatgct
    13321 aaaattgatt agcaaagaag gaggttatat tatgagttgg ttatggacat tgattgttgg
    13381 agccgtgatc ggagcaattg caggtgcgat caccagtaaa ggaaattcga tgggctgtgt
    13441 aacgaatatc attgctggac tagtcggttc aagtattgga caagcgattt tcggcgaatg
    13501 gggaccaaaa gctgcaggta tggcacttat tccatctatt ttaggcgcag tgattttggt
    13561 agcagttgtc tcatttttct ttggaaaacg atcataagtt tttacaaaat agtaaactaa
    13621 aaggagttgc agcgagactg tgactccttt ttggctttat ttatttaaaa taaaaagaag
    13681 ttttccggaa atttttattt aactgtcgtg tacgaagaaa agtttgctat aatggaaaaa
    13741 agcgtgcaaa gaaagaggga gtttcattat gatgctacgt gtagaaaaat tgcgcaaaaa
    13801 aatgcaagaa gaaaatttgg attcattttt ggttaccagt ccgtacaatc ttcgttactt
    13861 aacgaacttt acaggtacga caggattagc tgttattact ttggaaaaag cattttttat
    13921 tactgatttt cgttataccg agcaggcggc agcgcaggct caagggtttg aaattattaa
    13981 aaatgtcgga cctatttttg atgaggtagc aaacttagta cgcaaagaag aactgcactc
    14041 attagctttt gaggaaacaa cagtctcatt tttagaatat tcggttttag aagaaatcat
    14101 cgatgcggaa ttagtacctg tttcacagat gatcgaagaa ctcagagaag tgaaagatga
    14161 agaagaaatc gctattatcg aaaaagcatg tcatattgcg gatatggcat atgatcacat
    14221 cctgaaaatg atccaaccgg gcatgactga gattgaagtc gcaaatcaac tagactttta
    14281 tatgcgttct cttggcgctt caggtgtttc ttttgatacg atcgttgcta gcggattgcg
    14341 ttcggctatg ccgcatggtg tagcaagcaa aaaaatcatc gaacaaggtg acttgatcac
    14401 attggacttt ggttgctatt acgaagggta cgtttccgat atgacacgta cattcgctat
    14461 tggtgatcct ggggaaaaat taaaagaaat ttatcaaata gtcttagatg ctcaattagc
    14521 agttattgat gctgcaaaac caggaatgac tggtgtccaa ttagatgcag ttgctagaga
    14581 ttatatttca aaacatggtt acggggaagc atttggacat tctacaggac atgggatcgg
    14641 tttagagatc cacgaaggac caaacgtttc ttctcgtgca gaaaagcaat ttgtaccagg
    14701 aaatgtgatc actgatgaac cagggatcta cttaccagga atcggtggag tacgtattga
    14761 ggatgacttg ttgatcacag aagatggaaa tcgtgtgttg actcgttcac caaaagaatt
    14821 gatcatctta tagtttcatc aatagaagaa gtaagcgtaa aaatcagtgt tcacagactt
    14881 tgttatagaa aatcactcga tttaatgata cactatagaa gaacttttaa gctgtaagag
    14941 ataataaagt tgggtgtttt ctccaattca tagcagtttg cagctgatcg gcggcagttt
    15001 tcttaccacg tgatcactat gccgcatcat agagaattcg gatgtatctc gtgcttaggc
    15061 aaagggagga ataaataaat ggcagatgaa aaaaatttga ttttaaacgc aaaccaagat
    15121 ttaggagaga tcgtgatcgc tcctgaagtc atcgaagtaa ttatcgggat tgcagcttct
    15181 aaagtagaag gcgtatacgg aatgcgcgga acttttgcca gcaatgtcac tgaattatta
    15241 ggacgtgcag ctcacggaaa aggtgtttac ttagcaaacg aggaagatgg cttgaaagta
    15301 gatctttact gttatctaga atatggtgtt tcagttccta aagtagcaat ggaaatgcag
    15361 gaacgggtca gccagcaagt ccttttcatg acggatatcg atttatctga agtcaatatc
    15421 catgtagtag ctgttgttcc tgaaaaatta gaacaaccaa caatcgaaga actttttgaa
    15481 gacgaagagg aagagaatga ataaagaatt atcaagacac gagattcggg aaatggcatt
    15541 gcaggcttta tttccattag acttcaatgc agacctaaca aaagaagatg cgattttcaa
    15601 cgcaatcgaa ttagaccacc gcgacatgat caacgaagat gaatcagagt ttgttcctgt
    15661 ttatcttgat acgctagttg gcggtgtctg tgcaaaaaaa gatgaattgg ataaagtgat
    15721 tgaaaaacat ttaaaaaaca attggcacat caatcggatc tcaaaaatgg atttggtgat
    15781 cttgcgtatc gcgatttttg aaatgactta tgtgacagat gtaccagcgg ctgttgcttt
    15841 aaatgaagca atcgagttgg caaaaacatt tagtgatgac cgttcacgta aattcgtcaa
    15901 cggtgtttta tccaatgtat tgaaagaatt agaagctggg gcgtaagtcc tagctttttt
    15961 ctatgaatca tcataaacct gttataaatc gggaaaattc tatggtagaa tgaaaacaca
    16021 aaaaatatct gggagtggtt agatggcaga attgattaac ggaaaagaat tagctgaaaa
    16081 aatgcaggca gagattgctg aaaaaatcca aaaattgaaa gataatggta tccatcccgg
    16141 attagtcgtt ttattggtag gggaaaatcc agccagccaa atctatgtaa gaaataaaga
    16201 acgtgtagca aaagaaatcg gaatccattc attggtagag cgttatcctg agacgatttc
    16261 tgaagacgaa ctacttgcag aaattgaaaa atacaatcaa gatcctagtt ttcatggtat
    16321 attggtccaa ttaccattgc caaagcatat agatgaagaa aagatcctgt tagctatccg
    16381 ccctgaaaaa gacgtagacg gttttcatcc gatgaattta ggcagactcc ttgcgggtaa
    16441 accggacaag attccttgta ctccatacgg catcatgaaa atgtttgaag cttattctat
    16501 cgatccggcg ggtaaacgag cagtagttat cggacgaagc aatatcgtcg gtaaaccgat
    16561 ggctcagcta ctcttgatgg cagatgcaac tgttactgtt gctcattcta aaaccgaaaa
    16621 tctggctgaa ctggcacaag aagcagatat tttagtcgta gcaataggac gtggacattt
    16681 tgtcacaaaa gaattcgtaa aaccaggtgc tgtagtgata gatgtaggga tgaatcgtga
    16741 tgctaacggt aagctgatcg gtgatgtgaa atttgatgaa gtggagccta ttgcaggctg
    16801 gatcactcct gtgccaaagg gtgtagggcc aatgactatc acgatgctga tgtatcaaac
    16861 cgtaaaatct gccgaagctt ttggagcagg tgaatagatg gcacaagaat atttaactgt
    16921 cactacatta acgaagtatt tgaaaagaaa atttgatgcc gatccttatt tagaacgcgt
    16981 atatttaaca ggcgaaatat cgaatttccg tttacgtgcc aatgcccatc aatatttcag
    17041 cttaaaagat gaccatgcaa aaatatctgc tattatgttc aaaggagcat tccaaaaatt
    17101 acgattccag ccaaaagaag gaatgaaagt ccttgtcgtt gggcgtattt cattatatga
    17161 agcgggaggt tcttatcaaa tctatgtaga gcacatggag cctgatggaa taggtgcttt
    17221 atatcaagaa ttggaagaac gaaaagaaaa gctgatgaag gaaggccttt ttcaagggcc
    17281 taaaaaaatg ctgccacgtt tcccaaagcg aatcgcggtc cttaccagtc caagcggagc
    17341 ggttatccgc gatatcatca cgacggtgaa aagaagatat ccaattgtcc aattggtttt
    17401 gttcccaaca gttgtacaag gaaaccaagc agcggacaat gttgtcagga atatccagag
    17461 agtagaagag atgggcaatt tcgatacaat gatcatcggt cgtgggggtg gttcgattga
    17521 ggatttatgg ccgtttaatg aagagcgggt tgctcgggcg atttatcaag ctaatacgcc
    17581 ggtgatttca tctgtaggac atgaaacaga taccacgatc gctgatttag tagcagatgt
    17641 tcgggctgcc actccaacag cagctgcaga attagctgtt cctgttttgt cagaagagtt
    17701 gatgcgcatt caagaacggc aagctagatt gcagcaggcg tttgtacgcc agatccagcg
    17761 caaacaagaa cggttcgagc gtgctcgacg ttcctatgta tttcggcagc ctgaaagatt
    17821 gtatgaaggt cagaccatca aattagacca gttgaaacag cgattatatc aagctacaca
    17881 gcaaatttat catgcgaaag aaaaacaaac gatcgcatta agccaccaat tggaacaagt
    17941 ggctccgctt tatcgagtta aagcagctcg ccaagaaact gcttatctag aaaaacgctt
    18001 agaagagaaa atccaacaat atatgaaaca acagcagcag cgttttgctc aagctgttca
    18061 atctcttgat ttgttgagtc cattgaaaat catgggaaga ggctacagct atacgacgtt
    18121 aaatgatcaa gtagtaaaaa gtgtcaccga gattcatcaa ggggatttgc tgacgatcca
    18181 ttatcaagat ggaatcgtta aaggacaagt acaagagata gacaaaggag aataaaaata
    18241 tggaaaatcc aacttttgaa gagtcactgc aagaattaga aaaaattgtc atgcagttag
    18301 aacaaggaga cgttccatta gaatcagctc tagattcgtt taaacgagga atggaattaa
    18361 gcaaacattg ccaagataca ttagctaaag cagaagagac cttgacgaaa atgatgacag
    18421 aatcaaatga agaagttgat tttgacggaa acgaggaaag ctgagatgag attaactgac
    18481 ttttcagcta ttcatctacc tgctgtagaa aaagaaatct tgtctttttt agatgagcat
    18541 acaacggata gaggactcta tgatgcgatg agttattcag tgaaagcagg cgggaaaaga
    18601 atacgtccct tattactttt aacggccgtt gcttcctttg atgagcctat tgatgttccc
    18661 gtctatcaag tagcagctgc tttagaaatg gtgcatacgt attccttgat ccacgatgat
    18721 ttgcctgcga tggacaatga tgatcttcgt agaggaaaac ctaccaatca taaagtattc
    18781 ggcgaagcat tagctatatt ggccggagat ggtttgctta caggagcatt ccagctaatc
    18841 agtatggcac acttaggtaa ttctcctaag cttctgctgc ttcagcaatt agctgtttgt
    18901 gcgggatcac aaggtatggt tgctgggcaa gctgcagata tcgagggcga atcgaaaaag
    18961 ctttctttag aagaattagc atttattcat gaaagaaaga cagggcattt gattcgttat
    19021 gccttgctgg ctggtggcat attagcgaag caacctgaag aaatcttatt gctacttcaa
    19081 cgattagccg aacatttagg tttagctttt cagataagag atgatctact cgatgtgatt
    19141 ggtacaacga aaacattagg gaaaacagct ggtaaagatg aacgtatgga aaaaaatact
    19201 tacccgcgtt tattaggcct agaaaagaca cgtgaggcgt tggagattga gctgttatcc
    19261 gccaacaaga tcatagacaa attagaagag gacgtttttt cttttgatgg cgggctgatg
    19321 cgccagatga tcaagcaatt tgatgtggag aaatgaggga aagaatggaa aaagaacgcg
    19381 tagatgtatt agcggtcaaa caaggattat ttgaaacaag agaacaagcc aaacgttcag
    19441 tgatggctgg tttgatttac aacgaaaaaa acgagcggtt tgataaaccg ggagaaaaaa
    19501 ttccagtgag tagtgaatta agagtcaaag gtaaaaaact tccttatgtt tctcgagggg
    19561 gattaaaatt agaaaaagca ctcaaagcat ttgatctatc tgttgaagga aaaacattgt
    19621 tagatattgg tgcatccact ggcggattta ctgatgcagc tttgcaaaat ggcgcaaaaa
    19681 tgagttatgc tttagacgtt ggctacaatc agctagcttg gaaaataaga caagatccta
    19741 gagtcgtcgt aatggaaaga gtgaattttc gttatgcaaa acccgaagac tttaccttag
    19801 gaattccaga agttgctgtt atcgatgttt cttttatttc actaaaactg atgctgcctc
    19861 cattacatgc tattttaaaa gaagagggag aagtgattgc tttaatcaaa ccccaattcg
    19921 aagcaggcag agaagctgta gggaaaaacg gaattgttcg tgaccctcag acccatcaaa
    19981 aagttttaga agacattttg tcatttgcgg cacaaggtgg atatgatgtg ctggaattaa
    20041 gttattctcc gatcacgggg ggagaaggaa atatcgagtt cttggctcac ttgaggaaag
    20101 tacccgaaag cggaacaatc aattcagcta ttaatatggc agaagtggtt tcaaatgcac
    20161 acgaacagtt tgaccataaa taaattgtaa ctgtgaaaat caagtgagaa gtatgcggat
    20221 tcttctttat tataccaagg aaataagcta tcattgatta agaagaatat gctaaggagc
    20281 gaagacgtaa tgagaaaaaa agatcggcat cgtttgatta ctcgattgtt gagtgagcaa
    20341 gatatccgaa agcaggaaga atttgttgaa atcctgaaaa gcaaaggaat atctgttact
    20401 caagctacga tttccagaga tataaaggaa ctgaaactga tcaaggtacc ggcttcagat
    20461 ggcggctacc gttatagcat accagctgaa acaaacgaag atgtaaatac taaattggaa
    20521 aaactattaa aagacgcttt cgtggcagtt gaccaaatgg aaaaatttgt tattttgaaa
    20581 acattgccgg gaaatgcttc ggcagcagca aatttgattg ataaacgtta caagaaagaa
    20641 ttattttcga ttatcaatga tgacgataat gttttgatga ttacacgaac tgaagaccag
    20701 gcagttttat tgaagaaaga cttcctgcat tacctttaag ttgaggagcg aatgtgatgc
    20761 tacaagaaat cagtattact aactttgcga ttattccgga attgcggctt tcttttcacg
    20821 aaggaatgac cgcgctgacc ggagagactg gtgcgggtaa atccattatt atcgatgctt
    20881 taggtctgtt agcaggagga agaggttcaa gcgattatat acgtcaagga gcagaaaaat
    20941 gtgtgcttga agggttgttt gaactaccaa aacaagaagg attctctgaa ctgatggttg
    21001 aattagggat cgaaacagat gaggacaatc tgattgttcg acgggatatg tccttaactg
    21061 gaaaaaatgt ttgtcgagtg aatggtcata ttatcacatt agctaatctg agaagaatcg
    21121 gcagttattt agtagatatc caaggacaaa acgaacatca ggaactgctc cagcctgaat
    21181 cacatcttgc tttattggat cgttttggag atgtagcttt ccaacagaag aaaaagagtt
    21241 atcagcaaga atatatctct tatcgtgagc tggaaaaaag ggtacgaaaa gtgcagcaaa
    21301 atgaaaaatc ctatgttcag agaatagata tgctccattt ccagcaagaa gaaatagctg
    21361 ctgctgattt gcaagtcgga gaagaagaga aattaaaaga agaaagagaa aaacttagca
    21421 attatcaaaa aattgctgat ggattagctg caggttacgg agcactgacg aataatgaac
    21481 aaaacagtgt agatggtgtt ggcttggccg tttcagaact gcaagggatc gcccatctag
    21541 atgtggagta tgaagcaatt tatgaaaata tccaaagtgc ttactatttg ctgcaagatg
    21601 caatcggcga tatgagtcgt cagattgatt tattggagtt agatgagagt cgtttagaag
    21661 aagttacgca acgtttagaa ttaatccgac agttgaaacg taaatacggc gaatccattg
    21721 agtcaatatt ggcttactat gacgaaatca ccgaagagct agcttcttct gatttttcag
    21781 aaggtcaatt ggacaaaatg aaagaggaac tagagcaaaa agagcttcta cttcagcaac
    21841 aagccgcaga tcttcatgaa gcgcgtaaaa aaatcgcaaa agaattggaa aaatccattc
    21901 tgcatgaatt gaaaagtctg tatatggaaa acactgagtt tgaggtacgt ttcttaaaag
    21961 aagaaaatag acaattgaat catgatggat ttgaccaaat cgaattttac attacgacca
    22021 atccaggaga gccactgaag cctttggtga aagtcgcttc tggtggcgaa ctgtctcgga
    22081 tgttgcttgc tttgaaaact attttttctt ctgagcaagg tgtgacaagt attatttttg
    22141 atgaagtgga tacaggtgtg agcggaagag ttgcacaggc catcgcagat aaaatttctc
    22201 aaatatcgaa atactctcaa gttttgtgca tcactcatct gcctcaagtt gctgcagtcg
    22261 ccgactatca atattatatt gtgaaagcag tgattgatgg acgtacgcaa acttctgttt
    22321 cagagttgaa aacaaaagaa agagaagaag aaattgcacg tatgctggct ggaagtgaga
    22381 ttacaaaact cacactcgaa catgcaaaag aactattgaa actcgcaaaa aggacctgag
    22441 ccacctcgca gtggttcagg tccttttcag cagctgatag ctatatttta tttttacatg
    22501 acactcatta cagcagtcaa tacaacagat ccaatcgtta aaatcagcat tgtccaaatg
    22561 acaaattttg ttactttagc aaaagtactt gtttgttttt tttcacgcat tgttttcaac
    22621 ctcgtcatta attagttgat tcaagtgtac aatctttttt ccatttccac aagagggctt
    22681 ttttataaaa acttcccttt ttttaaataa taggtagtta cgatacaaat aaataatgtt
    22741 aatagaacta gccatataaa ggcatcatcc ctttcagcaa taggtaactt aatgttcatt
    22801 ccatagatac tgccgacgat tgtcggtata gttaaaataa tcgttaaaga agttaaaatt
    22861 ttcataatat tattcagatt attcgatacg attgctgaaa aagtctgatt catttgttct
    22921 actagtttta attgaatgcg agaagtagtc attgcctgct ctgtttccac caatacatca
    22981 cgtaaatgat ttttcaaagc atgattttct tggaaaagtt tattttgcgc tagttctttc
    23041 aatgcatcta aattagcaga agtcgcttct ctgaaataaa cgaggctttt ttggatatcc
    23101 atgatttgat aaagttgttt gttttcagtg gatacttgaa gtttggcttc caaagtatcg
    23161 cattgagatt gaagcgtagc taaataactg ttgtaagaga gtgtgacctg ccaaatgaat
    23221 tgaaagaaga tattgagatt aggcgaaata tcactttttg gaaaggtctg gctaaagact
    23281 ttctttaaaa acagtggttc atgattcgta attgtgataa gcttcttttt gggcgtcaaa
    23341 ataatagaga gcgggtatgt atccacttga aaataaccgc taggacttgt tttagcaaaa
    23401 ggatattgta acaataataa cgctggcttt gtcaactttg attgattgaa ttcttctgta
    23461 cgggaatttt ctgcatcgtc caatatggaa gtgatgtaat ctgacggaat ttcaaattga
    23521 tgtgtcagct gatgaatctc ttcttgatta ggacgttcta cacaatacca gatcgtttgg
    23581 tcagtttcct ttccggtatt acgcagtttc tcattatcta atgtaaaata tttgatcatg
    23641 attttcctcc tgttgcctgt ttcttttatt ataacggata gggaattgga atacatggaa
    23701 tgtgtttgta agatatagaa tataactgca taaatttaaa taaaatgaaa cagttgtgta
    23761 aagtatatac agtatacttg aagcaacgat aaaaaaggag tttacgttca tgggcacatt
    23821 cacaccactt atcttgtttt ggtatattta cccggtaatc gttttgtttg gttgccaatt
    23881 tcttgtaaaa ttattttcgt taaatcgtcg ttttaaaata aaagcccctg atttggcgat
    23941 tccttttttg tggggcggta tgcatgcatt gtctagaaat acagggacaa tttctatctt
    24001 accgtttttc tttatttctg ttttgctaat gggaatttgt attgctgttt tccaagcata
    24061 ttattatgaa gaaattattt atccgcgtta tttcaagatg acttggcgtt tggtttttct
    24121 gttgacaatc gtcttgtacg ctgtcttgat cattgtgaac atactttctt acctatgaaa
    24181 aaaatagaaa agctgtaaaa agtcttttaa aaggcttttt gcagcttgat ttttaaaaaa
    24241 atgtttatga ataaaaaaag ttgttcccgg atttcccaga aaaggtggta gaaagtgggg
    24301 gattgtggta gactaatttt agttagtgga ggaatggggg gctttcaatg ttgatgggtg
    24361 aattccaaca taatattgat gcaaaaggac gtttaatcgt gccctcaaaa cttcgtgaag
    24421 aacttggcga aaagtttgtt ctgacacgcg gattggatgg ctgtcttttc ggctatccga
    24481 tgagcgaatg ggaaaatctg gaagctaaac tgaacgaaat gccgttggct aaaaaagacg
    24541 cacgtacttt tgtgcgattc ttctattcgg ctgccactga atgcgaactt gataaacaag
    24601 gacgaatcaa tatacccagc acgttaagaa attatgcagc tttgacaaaa ggttgcgtag
    24661 tcatcggtgt atctaaccga attgaaattt gggatgagac acgatggcaa gatttttctg
    24721 cagcagcaga agagaacttt gacgaaattg ctgaaagtat gattgatttc ggactataag
    24781 aataggaaga ggctgactaa tgactgaaac atttcaacac tatacagtca tgttgaaaga
    24841 aacggtagat ggattgaaca tcaaaccgga tggcgtttat gtagactgta cactcggcgg
    24901 tgccggacat agtgaatatt tattaagcca actaagttct aaagggcatc tgtatgcgtt
    24961 tgaccaagat caaaaagcaa tcgatcacgc caagctgcgt ttagcttctt atgtcgaaca
    25021 aggtatggtc acatttatca aagcgaactt cagagaatta gaagaacgct tgagtgagca
    25081 tgtagataag gtagacggca ttttgtatga tctaggggtt tcctctcctc agctagatga
    25141 agcagaacgt ggatttagtt atcatcaaga tgcaccgttg gatatgcgca tggatcagtc
    25201 agctccttta tccgcttatc acgtaattaa tgaatattcc taccatgaat tagtaaagat
    25261 tttcttccgc tatggcgagg agaaattttc caaacaaatt gctcgtgaaa tcgaacgagt
    25321 acgaaaaaca aaaccaatcc aaacaactgg tgagcttgtc gaaatcatta aacaagtcat
    25381 cccagctccc gcaagaagaa aaggcgggca tcctgcaaaa agaatttttc aagctgtgcg
    25441 gattgcagta aatgacgaac tcggtgccga agaggcctct ttagaacagg caatccggtt
    25501 attgaaagtc aatggaagaa tcagcgtaat cacattccat tcattggaag acagaattgt
    25561 caaaagtatg tttaaagaat atagtacggt acaagatcta ccaccgggac ttcctgtcat
    25621 acctgacgaa ttccaaccag aactgaaagt cattactcgt aaaccaatcc ttccttcaga
    25681 ggaagagcta acagaaaaca atcgatcacg cagtgcaaag ttacgtattg cagaaaaaat
    25741 aagaatgaaa gaggagtaga atgatggcag aattaaagaa aatgcaagat tacgaatatg
    25801 atttacctga attggtggat cagccggaac aacaaatgcc tgattcttct tcgaatcaag
    25861 aaatcattct tccacaatca ccggcaaaac gattaaagca gatttctgct ttggaaaaaa
    25921 ttgctgtggc aagcgtggcg attgctattg ttgctttatg tgttttaaca gttatgctgc
    25981 gcacaaatat cagcggagct gaaaaagaaa tcacgtcaat acaaacagaa atcaatgata
    26041 aaaagcaaga aaaaacgagt ttagaacaag aaaaaaacga actttctcgt actgaacgga
    26101 ttaaacaaat cgcagaagaa aaaggattgt ctattaatga cgacaattta aggaaagtga
    26161 agtaaatgag tcttaaaaat aaaattcggc ggtttatgga aaagaaaaat ctaaatccga
    26221 tgaacaatcg caaaaaagta ggtatcattt tatttgctac gagtattgga ttgttctttt
    26281 tatttgccgt ccgtttctcg tatatcgtta ttggaggtca tgttgctggt acctcattag
    26341 ctgaaaaaac aaaacagttg taccaaggaa gtgaagtagt aaaagcaaaa agaggaacga
    26401 tttatgatcg taacggtgtg gcgctcgcag aagatgcctc ctcttattca attaaagcga
    26461 tactctcaaa aacatatacc tctggggata aaaaactgta tgttgaagaa aagaattttg
    26521 ataaaatagc agaaatcctg cataagaatc tttctataga caaaaaagat gcactgaata
    26581 ttttagagga cggcgcaaaa aaagagctgt atcaagttga atttggtagc tatgggaaaa
    26641 atatctccca agaaaccaag caaaacatcg aagcggacat gaaaaaagaa ggtgtcgctg
    26701 gtctttattt tgtcgaccat caggctcgga tgtatccaaa tggcgtcttt tcttctcatt
    26761 ttattggata cgcagttcca gacaaagatg agaatggctt agttggtaaa ctaggtttgg
    26821 agtctgctta caatgatatc ttgagcggta aagatggtaa gattatttat caaaaagata
    26881 attttcaaaa tcctctgcca ggtacagtgg cagaagaaga aaaagctgtc gacggacaag
    26941 atatctacac gactttagac agcagactac aaagttattt agaaacactg atggatcaag
    27001 taaatgaaga atatcagcca gaagaattga cagcagtcct gatgaaagca aaaaccggag
    27061 aaatcttggc aatgggacaa cgacctactt tcaatcctga aacaatggaa ggattgactg
    27121 gaaaagatgc tatctggcgt aatttcttgg tacaagatag ttatgaacca ggatcaacca
    27181 tgaaagtttt cactactgca gctgctatcg aagaaggaga attcaacgag aatgaaacct
    27241 tccaatctgg aaaaatccaa gtagaagatg ctacgatcaa tgaccatgac tttggtgaaa
    27301 aaggtgtgct gaccatgcgt caagctcttt catggtcaag taacgtcggg atggtcatac
    27361 tggaacagcg attaggcgga cgatggtata actatttaca aaaactggga tttggacaaa
    27421 gtactcattc tggattagat gatgaagtaa acggtgcttt acctacatta aatattgtcg
    27481 accgagcaat gagtgcttat ggacaagcgg taggtgtgac gaatttccaa atgatgaaag
    27541 ctttcacttc tattgcgaat aacggtacaa tgattcaacc tcgatatatc agcaaagtgg
    27601 tggatcccca aactggagaa gagcgaacaa ctcaaacaga agttctaggg caaccttttt
    27661 caaaagaaac aacagaaaaa gtccgtgaat atatgagaga tgttgtcgaa agcgaaaact
    27721 acggtagtgc ttatggtgta tatagtgtac ctggatacaa tgtatcggca aaaaacggaa
    27781 cagcccaaat cgcttctgat acagggggat atcaaacagg agatacagcc tacctgtatt
    27841 cgattgtgga gatggttcct tctgaagatc cagactatgt cttgtatcta acaatgaaac
    27901 accctaagac atatgatcga atggcattgg ctaaaatcgc aaatccattg atgaaacggg
    27961 caatggattt taaagaaact gaagaagatt cagatacaga aacaaaaaca gaaaaagtat
    28021 ccgtagccga ttatcgtaat cttgaagcgg acgttgcagc agctgatgct caaaaaagtg
    28081 gactgcaacc tgtagtgatc ggtaatggta aaaaagtaca aaaacaatct actgccaacg
    28141 gtgatcagtt gatctctgga gaaaaactga tcctatatac tggtggtgat aagctgatgc
    28201 ctgacgtaac aggatggtca aaagcagata tcatgaagct ggggaaaatc ctcggtattg
    28261 aagttagttt tgatggagat ggttattgtg tcaaacaaga acttgctcca tatgaaaaaa
    28321 tcactgaaga caaacttagt tttactttag aagaataaga agagaaatag gagagaaaag
    28381 agcatggaat ggacacaagc attgattccc attgtcagca gttgtgcaat gacaattgcc
    28441 gcaatgccgc tatttattgg ttattttcaa atgaaaaagc aaggtcaggc gattcgtgag
    28501 gagggaccaa agtggcacaa tagcaaagcc ggaactccta caatgggcgg cttagtgttt
    28561 ctgatcggct ctattttgac aggaatttgg gttggagctt ggcaaaaaca gctaactcct
    28621 actcttttca tcttgctttt tgtattagcg ttatacggag taatcggctt tcttgatgat
    28681 tttataaaaa tattcaagaa aagaaacatg ggactgaact cgaagcagaa attgattgga
    28741 cagattctag ggggaatcat cttttatctt gtttaccgtt ctgaaggtta tccaggtgta
    28801 ttgaatttct tcggactaga tctaccttta ggaatcattt atggtatttt cgctctcttc
    28861 tggcttgttg gattttcaaa tgcagtaaat ctgacagatg gcatcgatgg attagttgct
    28921 ggcttaggaa gtatctcttt tgcagcttac ggtctaattg cttggcatca gcatcagtat
    28981 gatgtattaa tcatttgctt aagtgtttta ggaggtttac tgggtttctt cgtctacaac
    29041 agaaaacctg ccaagatttt tatgggagat gtcggttcat tagcgttggg cggactttta
    29101 gcggcaattt ctataatgct aaaccaagaa tggacattgc ttttgattgg tttgatctat
    29161 gttatggaaa cagcaagtgt gatgcttcaa gttacttcct ttaagcttac aggaaaacga
    29221 atttttaaaa tgtctccaat tcatcatcac tttgaaatgt gcggatggtc ggagtggaag
    29281 attgatacag ttttttggtt gatcagcatt gtgacttcgt tgatcacatt atggatcgtt
    29341 tggtaggaga tgacgggaaa tgaaaaaaat atcaacatat gaaaataaga aagtgcttgt
    29401 cttaggactc gcaaaaagcg gagtaagtgc ggccaaatta ttacatgaat tgggcgcact
    29461 tgtcaccgtt aatgatggaa aaccttttga cgaaaatccg gaagcacaag agctgctttc
    29521 tttagggata aaagtaataa caggtagtca tccaatcgaa ttgttggatg aagaattttc
    29581 attgatggtc aaaaatccag ggattcccta cagtcatcct ttagtagcaa aagcccaaga
    29641 aatgggtatt ccggtaatta cggaagttga gctagcttac gaagtagcgg aatgcccaat
    29701 tattgggatt acaggaacga acggaaaaac aacgacaaca acgatgacag gactactact
    29761 gaatgcagga gcagatcaag gaattgcacg tttagcgggt aatattgggt atccagcaag
    29821 tggtgtagcg caagaagcaa aatcagaaga taaaattgtg atggaattgt ctagcttcca
    29881 actcatggga atcacagatt tccgccctca tatagcggtg attaccaata tctatgaagc
    29941 acatattgat tatcatggta caagaaaaga atatgtcaaa gctaaatgga accttcaaaa
    30001 aaacatgaca gaaaaagatt atctgatcct taactggaat cagtcagaac tacaagaatt
    30061 agcgcaaaga acaaaagcac gcgtactgcc tttctcgaca aaagaagtgt tggaagacgg
    30121 tgtgtatgct gacgattact caatatacta taaaaaagaa aaaataatgg aaatatctga
    30181 acttggcgta ccaggtaagc ataatgtaga aaatgcacta gctgctattt ctgtagcaaa
    30241 attgtatggt atatcaaatg aagctatacg agaaacgttg cattttttcc atggcgtacc
    30301 acatcgtacg caatatgtag gagaaatcca aggcagaaaa ttttataatg attcaaaagc
    30361 aaccaatatt ttagcgacta aaatggctct aagtggattt gaaacgtcta aggtcgtgtt
    30421 attggctggc ggattagaca gaggaaatac atttgatgaa ttaattcctt cattaaaagg
    30481 aatcaaagca atggtcgtat ttggccaaac aaaagaaaag ttgatggatg ctggaaaaaa
    30541 agcaggcatc gagacaatag taacagccga ttctgtagaa caagcagttc ctttagcatt
    30601 agaaaacagt acagatggtg atgtagtttt attatcacca gcgaatgcca gctgggatca
    30661 atacccaaat tttgaaacta gaggaaacag attcatggaa gctgtgaacc gattaaaata
    30721 gaaaaagtag aagcgagcga taaaaaatga aaattttggt tacaggcgga ggaacagggg
    30781 gacatattta tccagctctt gcctttgtca attatgtaaa gactcaagaa ccgaatgctg
    30841 aattcatgta tgtgggagca aaacgaggtc tagaaaacaa aatcgtccct gatacaggaa
    30901 tgcctttcca taccttggag attcaaggat ttaaacgaaa aatttccatg cataacatca
    30961 agacggtccg attgtttctt aagagtatcc gtcaagcaaa aaaaatcctc agtgattttc
    31021 aaccagatat cgttatcggt actggaggat atgtttccgg agcggtagtg tacgctgcag
    31081 ccaaaatggg tattccaaca gttatccatg aacaaaacag tgtaccaggt gttaccaata
    31141 aatttttaag ccgatatgtg gatcggatcg ctctttcatt taaagatgcg gcttcctttt
    31201 ttcctgaaaa caaagcagtt cttgtaggta atcctcgagc acaagaagta gcagatacta
    31261 aaaaatcaga tattttgaaa acatttgatt tggatccaga aaagaaaaca gtcttgattt
    31321 ttggcggtag tcaaggagca cttaaaatca atcaagcagt atcagttttt ttaagtgaat
    31381 atattccttc ttcttaccaa gttttgtatg cttcaggaga tagatattat gaagagatcc
    31441 taaataaaat aaatcctgaa atacaaaata ttagtgtcca gccttatatc aaaaatatgg
    31501 cagaggtcat ggcttccagt gatctgctgg tggggcgtgc tggtgcaact tcgatcgcag
    31561 aattcacagc tctggggtta ccggcagtgt tggttcccag cccatatgtt acgaatgatc
    31621 atcagactaa aaatgcaatg agtcttgttc acgcaggagc agcaaaaatg attgcagata
    31681 atgaactaac aggtgaatca ctaagtcaga cagtcaacga aatcatgggt gatgaagaat
    31741 tgcaaaagca aatgtgccga gcttctaaag aacaaggaat ccctgatgct tcaaaacgtc
    31801 tttatgactt agtaaaacaa ataatcaaaa aataaaggcg gtggtcttag tgagtaagaa
    31861 aaaagaacca aaaaaattgt ctcatccgtc ttctgatgaa aacaatttga ctccttggca
    31921 aaaggctaac cgaaaatatc tggcagaaca agagatgaaa aaccaagagc caaaaaaaga
    31981 agacataaca ttagatacac ccctttcaga ttctgaagaa gaaaaaacgg ttcaagagga
    32041 acagcaagaa gaaagccagt ccgagaaaaa aatcgaattt tttgaagaga tcaagcaaaa
    32101 gaaaaacggt ggaccatata acggttcttt tttgaaccgg ctgcccaatt taaaaagtca
    32161 acggcacaaa gtactatttc gtcggttagc tttgatcatc actgtattgg gtataccgct
    32221 gatttttttg atctattatg tttctcctta tagcaaatta caagcagttg cagtatcagg
    32281 caataagaca gtcaacagcc aagaaatcat ctctgataca aaattgtcat taggagaaaa
    32341 tgtttgggga cagtattttc atcgatctac ttatatcgat cgccttaaaa aagcacagcc
    32401 aagaatcgaa acagcaaata tacatttcaa agggatgaat gaattcgatt tggatgtaac
    32461 agaatataaa gagattgctt tgatcgcaaa aaacaatcaa tactacccag tgattgaaaa
    32521 tggaacagta ttagatgaaa aagtagcaaa tcctacaaaa aatctcccta tcttggaaga
    32581 gttcaaagac tctgcaaaaa tcaaagaatt aacaaagcaa tacaatcagt tatcatcaga
    32641 attacaaaaa gcaatttctg aaatcaagta cacgccaaga gcaagcaata aaaatctgat
    32701 ccaattgaat atgaatgacg gcaatcaagt tattgtaaat atcaataatc ttgcgaacca
    32761 aatgaaatat tattcgcaag tagccaaaga tatggatgaa aaaggtgtga tcgacatgga
    32821 agttggtatc ttctcgcatc cttacggtag cactcaagaa acggaagaat cgggccaatc
    32881 ggaagaaaca actgaaagtg aagaaaatgc ggatcaaaca aacgaatcaa gtgaagaggc
    32941 agaaacgcaa aaagcacaaa cacctggatc gtctagcaca aacggatcat ctgattcaaa
    33001 tgattcaagt gaaacaagtc aaatagaaga taatccaagt acaactccgt caagtttaaa
    33061 tgaaaattga taaaacaact ttaaaaagta agaatgtact ttctaaaaac cagtaattta
    33121 tggtagaatt ggaagcagtg aatatatcta ttaataagta tattttaaaa cggacgctag
    33181 ttgttcgctt gtatatggaa tagtaagtag gagggaatcc cattcatggc aaaaacagga
    33241 atgtatgtag gccttgatat tggaacgaca tctgttaaag ttgtcgtggc tgaatatatc
    33301 gacagccaaa tgaatattat tggagtagga aacgcaaaat cagaagggat taaccgaggc
    33361 attatcgttg atatcgataa aacggtccaa gcgatacaac gagcagtacg acaagcagaa
    33421 gaaaaagcag gtatccagat caaaggcgta agtgtcggtt tgccagcaaa catgcttgaa
    33481 gtagaaaatt gccagggaat gattgcagtt aacggagatt caaaggaaat caccgatgaa
    33541 gatgtcagaa atgtggcttc agctgccctt gttcgttcaa tacctcctga acgtcaaatc
    33601 gtgtcaatat tgccacaaga ttttactgtc gatgggttcg agggaatcaa agatcctcgt
    33661 ggaatgatcg gcgtaagatt agaaatgtac ggtctgttgt ttacaggacc taaaacgatc
    33721 atccataaca tacgtaaatg cgtggaaaat gccggattaa tcgtcaacga aatggtgatt
    33781 actcctttag cgctgactga atcaattctt tctgacggag aaaaagattt tggtacgatc
    33841 gtcattgata tgggtggcgg ccaaacaaca acggctgtta tgcatgataa gcaattgaag
    33901 tttaccaatc ttgaccaaga aggtggcgaa tttgtcacta aagatatctc tattgtatta
    33961 aatacgtcat ttaacaatgc agaagcgttg aaaataaact acggggatgc atacccagaa
    34021 cggacatcac cagatgaaga atttccagta gatgtgattg gtcagtcaga accagtgaaa
    34081 gtcgatgaac gctacttgtc agaaattatt tcagctcgaa tggaacagat tttcaacaaa
    34141 gcaaaagatg cattggacca aatcgaagca ttagagttgc caggtggcgt cgtattgaca
    34201 ggcggagctg ctagtcttcc tggtgttgtg gacttggcac aagaaatctt cggcgtaaat
    34261 gtaaaattgt atgttccaaa tcacatgggg ctgagaaacc cggtatttac aaatgtaata
    34321 agtatcgtgg attattcagc aaacttgagt gaagtgtatc aattggcaaa aattgctgtt
    34381 acgggtgaaa cctcagcagc tcgtcaaatg gtagttgaac aagaaacaac aaacacatat
    34441 gagagctatg aagcacctga agaaacaatc tacgatgagc cggaaccaaa agagtccgga
    34501 gaaaatgtaa aaaataaaat caagggtttc tttacaaata tttttgatta attcatcctg
    34561 ggaggaataa gatatggaat tctcaattga taataatatc aatgacggcg cagtaatcaa
    34621 agtcatcgga gtcggtggtg gcggtggaaa tgctgtaaac cgaatgattg aagaaaacgt
    34681 taaaggtgtt gaattcatta cagccaacac agacgtgcaa gcattaaaaa attcaaaagc
    34741 tgaaacagtt atccaattag gccccaaata tactcgtggt ttaggtgctg gttctcagcc
    34801 agaagttgga caaaaagcgg cagaagaaag tgaacaatcc ttacgcgaag ctttagacgg
    34861 tgcagatatg attttcatca ctgcaggaat gggtggagga accggtacag gtgctgcacc
    34921 aatcgttgca ggtatcgcga aagaattggg cgcattgact gttggtgtcg tgacacgtcc
    34981 attcactttt gaaggaccga aacgtggccg ttttgctgca gaaggtatcg ctcgtttgaa
    35041 agaaaatgtg gatacattat taattatctc aaataaccgt ttattagaag ttgtagataa
    35101 aaagacaccg atgcttgaag ctttccgtga agcagataat gttttacgac aaggtgtaca
    35161 aggtatctct gatttgatca cagcgccagg ttacgttaac cttgactttg ctgacgtgaa
    35221 aacagtgatg gaaaaccaag gtacagcatt gatggggatc ggtgtggcta gtggagaaga
    35281 tcgcgtagta gaagcaacga aaaaagcaat ctcctctcca ttgcttgaaa catctattga
    35341 tggagctgaa caagtacttc ttaacatcac aggcggtttg gatatgacat tgttcgaagc
    35401 acaagatgct tcagatatcg tagcaaatgc agcaactggc gatgtaaata tcattctagg
    35461 tacatctatc aatgaagaaa tgggcgatga aatccgtgtg acggtcatcg caacaggtat
    35521 cgatgaatca aaaaaggaac gcaagtctag ccgaccagct agacaagcac aaatgcaatc
    35581 acctgctcaa aagactgtat tagatatgga tcaagctaaa ccaacttctt cagaagaaga
    35641 aaacagtttt ggcgattggg atatcagacg tgaacaaaat gttcgtccta gagttgatga
    35701 ttccaatttt gacaatatcg aaaaaaaaga atttgataca ttcaaccgcg aagaaacaaa
    35761 atctcatggc gatgatgaat taaatacgcc accgtttttc cgtcgtaaaa aataaaggag
    35821 aggaagagca catgattgct gacaacttgc agaaaatcaa gcaagaaatt caggattcgt
    35881 gtgctcttgt tcatcgtgat ccgaaagaag ttacattagt agctgtaaca aaatctgttg
    35941 aatccgctca agctgaagaa ttggttagag ctggcgttat gaattgtgca gaaaaccgag
    36001 tagataaatt attagaaaag aaacaagatt tatttgattt tcctgaaata aagtggcatt
    36061 taattggtaa tttacagcgc agaaaggtaa aattgattgt aaatgaaatt gattactttc
    36121 atgcgttgga cagtttaaga ttagcagaag aaatccagaa gcggttagag aaagaattat
    36181 cctgttttat tgaagtaaat gtttctggtg aggaatcaaa acatggaatt tcaccagatg
    36241 agcttttacc gtttgtggaa tctttagctg cctgcacaaa aatcaaggtt gttgggttga
    36301 tgactatggc gcctaaagaa gcttctgatg agcaaatcca taaagtgttt ggtacgctga
    36361 aagaattgcg tgatcttgtt caagcaaaag gttatgccca tgcaccatgt agtgaattaa
    36421 gtatggggat gagtcaagac tttcaaattg cgattgaaga aggtgcaaca tttatccgtg
    36481 taggaacagc gctattcaaa gacgaataaa ggaggaacaa aaatgccgct tttaaacaga
    36541 ggagcaattg ctaatttttt cggattagct gatgaggaag agtacgaata ccaagattat
    36601 cctgaaacaa caaaaaaggc tgtacagcca actaaaaaaa atacaacaca gaatcaatca
    36661 actcattctg tatctgctgc gaatactcat gaaaaacaaa aaacggcgac ttcacgtcct
    36721 gtaacgcatg aaagtattca gccggctgaa aagaaaaaag taattgaact acatcaatca
    36781 cctacaaaaa ctgtagcgaa agaaagaaca gcttcaagaa cctctacaca acctaaagca
    36841 aatcttgctg gaaaaatcac tgtaatggaa ccaagagctt attctgaagc tatggcgatt
    36901 gctaaacgaa tcattgctgg agaagcaacc ttagtgaatt ttcatttagt agaagaaaaa
    36961 caagcgagaa gaatcgttga ttttctaaca ggaacagttt atgcactaga tggagatatc
    37021 aaacgtgttg gagatgagat ttttctttgt acaccgactg ggatggagat tgacagttca
    37081 accgctcaat cttttgcaga tgcgaatttg tttgatctgt aagaggagga actacgattt
    37141 atactattgc actattactt catttacttc aacgggcagc ctatttctat agcattatct
    37201 tgatggtata tgcattgctc tcttggtttc caggcgggta tcaatcaggg ctaggacgat
    37261 tattagctaa gatctgcgaa ccgtatatca gtttgttcga tcgtctgcct ttgaggattg
    37321 gacccttgga tttcacaatt ttaggggcaa tcctgatctt gaacttagcc gtacaaggtt
    37381 tagcacagat cgttatatgg ataactgtcc atttagttta ttgattgaag caacaaaatc
    37441 tttattctca gaatagatag gaggggaaaa ttttggatgc aaatgtatac cagcactttc
    37501 gggcagatga acgtacattt atcgattcgg taggcgattg gattgaacag gttcaaggac
    37561 aatatgcacc ctatctgacc gaatttcttg acccaagaca atcctatatt ttagaaacac
    37621 tgattcgaca aagcagtgat ttgcgtttca tgttttatgg cggatatgaa caggcagaaa
    37681 gaaaaagatg tttgattttc cccgattatt acgaacctgt agaggaagat tttgaaacag
    37741 cattgtatga aatccattat cctagcaaat ttgccagcct aagtcatggg aaaatccttg
    37801 gaactttagt gggaacgggg atcaaacgct cttactttgg cgatatcatc tcggacggtg
    37861 atcgctggca agtatttatt gcaaatgagg tggatcattt tgttgctgca caagtaacaa
    37921 agattggaaa ggttgctgta cgcttggaac caatcaaata cacagaaatg attatgccga
    37981 aagatagctg gacacaagaa agaggaacag ttagttccat gcgactagac agtgtgatct
    38041 ctgagattta taatatttcc agacagcggt ccaagcagct gatcgaagct ggtaaagtaa
    38101 aagtgaactg gacagaaaat acaagacctg attttgtact tgaactatta gatattattt
    38161 cagtcagagg ttatggcaga gtccagatcc aagaacttga ggggaagacg aaaaaagaaa
    38221 agtacagggt actgttcggt gtattacgta aataaaataa aaaggtggtt tactcatggc
    38281 tttaactcca ttagatatcc aaaataagac attcccgaca aaaatgcggg gatataatca
    38341 agacgaagta gatgatttct tagatttagt tgttcgtgac tatgaggaat tgactcaaag
    38401 aaatcgagaa ttagaaaaag cagtcaaaca ttctgaagaa aaacttgagt acttcaatga
    38461 attaaaagat gctttgaacc aatcaatcat cgtagctcaa gatacggctg ataaagtaaa
    38521 aacaagtgca agcaaggaat ccgaagtgat cgttacttct gcacaaaaca aagcagatga
    38581 gttagttgcg aacgctgaaa aacgtgcaca tcaattaaca actgatgcag aagaaaaagc
    38641 aagaaaaatc ttgacagatg caacagaaaa agcaagacag cttgcgacag aaactgaaga
    38701 tcttaagaaa aaaacacgag tattccatca acgtatcagc ttgatgcttg agtctcaatt
    38761 agaacaagtg aaaagtccgg aatgggatga aattttacaa ccattctcca gctacgtgac
    38821 tgacagccat gaagtgatca aagaagtttt agcaaaacag cttgacaatg aaaatgatac
    38881 tgatgtaaac tcagacacag gagtcataga agcacctatg acggcatttg atacacaggc
    38941 tattgatatc tcgatcgtgc ctggtgaaaa tgaaaacgag tagaacagaa aagcactttg
    39001 ttattcttaa agcgagccga tgagagtgga agttcggtaa gacactgcat ggtgaaagat
    39061 cctctacttg agacctgctt ttccgaaatt agagtaagaa gcagcggctg acctcgttat
    39121 tggtcacaga gagtttgcac tagatagatg caactaaatt tgggtggtac cacggcatgt
    39181 tcgtcccttg cggctgaaca tgcttttttg tgtccggaaa acaaataaag gagtgtatgg
    39241 ttgaatgaaa atgaaagaaa ccttgcaatt aggaaaaaca aaattcccta tgcgtggtaa
    39301 tttgccaaac cgagaaggcg agtggcaaaa agaatgggaa gaaaatcgaa tctaccaaaa
    39361 aagacaagag ttgaacgaag gaaaacctag ctttgttctt catgatggac ctccatatgc
    39421 caatggtaat atccatttag gacattcatt gaataaaatc agtaaagaca ttatcattcg
    39481 ttcaaaatca atgtctggct atcgtgcacc atatgttcca ggatgggata cacatggatt
    39541 gccgattgaa caagtattgg ctaataaagg cgtgaaacgt aaagaaatgt ccatggcaga
    39601 atacttggaa aaatgtcgtg aatatgcact ttcccaagta gataagcaac gagcagattt
    39661 caaacgccta ggagttgcag gagattggga acatccttat gttacattag atccttctta
    39721 tgaagctgca caagtacgcg tatttggtaa aatggctgaa aaaggctata tttacaaagg
    39781 attgaaacca atttactggt ctccgtcaag tgagtcttct ttagcggaag ctgaaattga
    39841 atataaagat gtaaaatctc catctatttt tgtagctttc aatgtcaaag acggcaaaga
    39901 tattcttgat gaagatacag cttttgtcat ttggacgaca acaccatgga cattgccagc
    39961 aaaccaaggt attgctgtaa atccaaaatt tacgtatgta ttggtggaag ccgatggccg
    40021 taagtttgtc gtagcgaaag atttgctaga aacagtccaa tcagaaatcg gttgggaaaa
    40081 tgtaacagtt ctaaaagaat ttgctggaca agaaatggaa tatatgactg caagtcatcc
    40141 attctatgaa cgtgaatcac ttgtgatttt aggcgatcat gttactttag acgctggtac
    40201 tggattagtc catactgctc caggacatgg ggaagatgac tatatcgctg gtagcaaata
    40261 taaactatct gtagtgtcac ctgtagactc aaaaggattg tttacagaag aagcacctgg
    40321 atttgaaggt atcttttatg acaaagcaaa tcctatgatc actgatctat taaaagaaaa
    40381 aggagctttg ctgaagctag atttctttac tcatagttat cctcacgatt ggcggacgaa
    40441 aaagcctgtg atctatcggg caactccaca atggtttgca tctatcgata aattccgtca
    40501 gaatattttg gatgaagtag aaaaagtaga gtgggttatc ccatggggta aaacacgttt
    40561 gtataatatg atccgtgacc gcggcgattg ggtaatttcc cgtcagcgtg catggggagt
    40621 accacttccg attttctatg ctgaaaatgg agaagcaatc atcacaccag aaacaatcga
    40681 acatgtggca aaattatttg ctgaacatgg atcaattatc tggtttgaaa aagaagcgaa
    40741 agaacttctt cctgaaggat ttacccaccc aggttctcct aatggcgaat ttacaaaaga
    40801 aaaagatatc atggatgtct ggtttgattc tggttcttct catgaagctg ttttacgtca
    40861 gcgaccagaa ttatcattcc cagcagatat gtatttagaa ggatcagacc aatatcgtgg
    40921 ctggttcaac tcaagtatca cgactagtgt ggctatcaat ggtgtagcac catacaaatc
    40981 agttatttct caaggatttg ctttagatgg tgaaggccgt aagatgagta aatctttagg
    41041 aaacatcatt gcaccagaaa aagtgatcaa acaatttggt gcagatatcc ttcgcttatg
    41101 ggtatctagt gtagattatg aagcagatgt tcgtgtttct atggatatct taagtcaagt
    41161 ggcagaagta tatcgtaaaa tcagaaatac gatgcgtttc ttattagcga atacagaaga
    41221 tttcgatcct aaaaaagata ctgtttcgta caatgattta cgttctgtag acaaatatat
    41281 gaccgttcga ttgaaccaag taatcaaaga aatccgagaa gaagggtacg acaaatataa
    41341 cttcatgcat atttaccgta cagttatgaa cttcttgacg gttgatctat cgtcattcta
    41401 cctggatttt gctaaagatg tagtttatat tgaagcagaa gatgattatc aacgtcgttg
    41461 catgcaaact gttttctatc aaacagctgt agctttgaca aaattattga ctcctatcat
    41521 tccgcataca gcagaagaaa tctggtcatt cctgaaagaa gaagaggagt acgttcagtt
    41581 aagcgagttt cctggttatg aagagtttgc aaaccaagaa gaattgatgg atacctggac
    41641 tgctttcatg gacttccgtg ataatgtctt aaaagcattg gaagaagcaa gaaacagcaa
    41701 attgattggt aaatctttgg aagcaaaact aactgtttat ccaaaacaac aagttcgtga
    41761 aatgttaaaa gctttggatg cagatattgc acaattattg atcgtttcac cagactactt
    41821 tgaagttatg gcagcagatg aacaagtgcc agataacgct atggtatttg atgatgttgc
    41881 tattacagtg gaaaaagcag acggggaaac ttgcgatcgc tgtcgccaag ttcgtaaaga
    41941 tgtaggagta gatgaaaaac ttcctcatct atgcggtcgt tgtgcaaaaa tcgttgaaga
    42001 cttctatcct gaagctgtag ctgaaggatt cgaagaaaag taaaagggaa aagggggtgt
    42061 gacatgttct ttgtcacacc cccttttgtt atcaatcgtt taattttcca cgttcacgat
    42121 accagttatc tggttgccag atccattcga atccgtcttt ttcaagtagt tcgaaagctg
    42181 ctttaggtcc cattgatcca gctttgtagt tgggaaaatc cggttgtgtt tggtcccaag
    42241 cttttcgaat gacatccacg atatgccaag attgagcgac ttcttcccaa tgagagaagt
    42301 tcgttccatc accattcaac gcatctaata gtaatttttc gtaagcttct gggctatttt
    42361 caaccatttc tgcactattt ctaaattcca atttgattgg ttccgtttca aatccttgcc
    42421 ctacttcttt cccattcaag gataaagaaa atccttcagt tggctgaata tagatcgtta
    42481 gaacattagg tggaagtgtc gtatcgtcac atggttcatc gacagatgtt ttgaatacat
    42541 taacaggaac ttgtttgaat acgatgttga tccgagtccc tttttcagtc aaacgtttgc
    42601 ctgtacgtac atagaaagga acacctgacc aacggaagtt atcgatagtg aatttgcctg
    42661 cgacgaatgt ttcagtctct gattgagcat ccactttgtc ttcttcgcga tagcccttga
    42721 attgtacacc atccaattca ccttgagcat attgcccgcg aacgaagttt tctaacactt
    42781 cttcttcgtt ataaatgcgg atagctttca atgctttgat cttttctgta cggatttctt
    42841 tttcggaaaa tgctactggc ggttccattg caagtaatgc tacaacctgt aaaatatgat
    42901 tttgcaccat gtctttaagt gcgccactat gatcgtagta gcccccgcga tcttctacac
    42961 cgaggttttc gccaaaagta atttggatat tgtcgatata gcgattgttc cattgagcct
    43021 caaaaatatt attggcaaaa cggatagctg agatgttttg gatcatttct tttcctaaat
    43081 aatgatcaat cctgaaaata tcttgttctg ggaatacttc tctgatttgc tggttcaatt
    43141 caaaagcaga ctggtaatca gaaccgaacg gtttttctat gatcaaacga ttgaagccat
    43201 tttttgtcat aatattttgt gatttcaaat gagaaacgat cgttccaaaa aattgaggtg
    43261 ccatagccaa ataatagaca cggttaccgt ttaattgata gtgtccatct aatcggtcag
    43321 acaattcttt taatgtctga taatgcgttg aatcatttac atcatgggat tgatagtaaa
    43381 aatggcttgc aaaggtagtt gcttcttcat ctgaaggatt caatgcacgg attgtttctt
    43441 tgacaacttc acggtagtgc tcatcgctcc agggacggcg agcggttccg attacagcaa
    43501 aatgctcgcc taaatttcct tttctgtaaa ggcggaacaa agacgggtac aatttacgct
    43561 gggctaggtc acctgtaccg ccgaatatcg taaacaatac atttttttca ttagacatgt
    43621 ggatacactc ctttatattt tatcgacaaa aatcgtgctg gccgccttaa agctgacggg
    43681 gattttcttt ctatcatttt ggatagtgat cggtccttca taagctgcga tgtcaacgat
    43741 ctcatattct tcgttgatat tcaggtcaat agtgactaag taatccaaca attctttttc
    43801 atcaagcact cgtgcgatac gcacttttgt accgacagga taggactcca atgtttgtcg
    43861 gcgttcttca tggacaactt cctctttttt aggaatcgat cctccatgag gacaatgttc
    43921 aggatgattc aaatagtttt ctaaatgatc tgcaagatgt tccgaagtca catgttccaa
    43981 tacttcagca tcatcatgga cttcattcca gctatagtct aaatgttcaa ctaaaaaaac
    44041 ttcccataag cgatgtttgc gaatcagact gctagctttt tgcagtccgc ttgcagtcaa
    44101 ttgtacacct tgataagggg aatgttctac aagctcttct ttcacaagtt tagaaatcat
    44161 ttcactgaca gatgcagctg agacatctag actagatacg atttgtttat tgttgacttt
    44221 cgttgtatct ccacctagtt ctaaaatgat tttcaaataa tcttctcgat ttggtgtcat
    44281 attcgatcat cctttcatgg taaaaaacca tcattagtaa gtgtaacaaa aaaaacgtta
    44341 attgactatt tttctaaata aaaaacaaag gatttttaga cgtttttaca acaaaaaacg
    44401 aaagatgata gagaagactc attctttcgt ttataaagga tatatatata cattgggagg
    44461 gatctatttt tcagataaca acttatatga agtgtctgca gattcgtcat aaagagtttg
    44521 ttcgggataa gaaaagataa gataaaaacc atactgatca cgtacgacta aacgatattc
    44581 atctatttct accacagatg tgccaagaga ctgaccgttg atcaaaaacg tgtaatcaga
    44641 tgtaatctgt aattgttgtt tttgctggct atcactaaag aaccatgtac cttgatactt
    44701 tgagagtcct tctgactttt ccgcttgttt tttctttttc aagtaaataa aacttgagag
    44761 aacggtaatg agtgaaccaa gaaaatagat catttttttt atcaccaaat cactccttta
    44821 tgagaaaata atcgcataga catattcttt cttctttatt tcattgtacc gaataaaagc
    44881 taaaatttct ctaagacttt cgactggttt ttttactgtt tttctacaaa ttatgatagg
    44941 atacatagta ggaagaaagg tgacataatt tgaagtatca gcaagtggaa atagcttttt
    45001 ttattgaccg cccgaatcgg tttatagcac attgtatgaa aaaagacgga gaaataatta
    45061 ttgcgcatgt caaaaataca ggtagaggaa aagaggtcct gataccaggc gctgaagtgg
    45121 ctattgtatt tgctcctgga cctaaacgga aaaccgccta tgatctgatt gctgtaaaaa
    45181 agaagggtga ctggtttaat atcgatagtc aattgcctaa tcggctggca attgatggta
    45241 ttttagacgg cacgatcctt ttaccaggat taactggtcc atttacggta tacaagcgtg
    45301 aagtaactta tcttcactcc aaatttgata tttatttaga gacggtggtc ggacaaaaag
    45361 catttgttga agtcaaaggt atgacacttg aaaataaagc gattggtgct tttcctgatg
    45421 cgcccacact cagaggattg aaacatatag gagaattaac gtatgccgca caagatggtt
    45481 atgccgctta tgtcttgttt attgctcagt tcgaacatct tcatctggca acgatccatg
    45541 aagaaatgca accagcacta gctgatatgg ttcgacatgc gcagcaaagc ggagttcaaa
    45601 tcctcgccta taattgtcaa gtatcggcag atgaggtaac tgtcaagcag gccattccat
    45661 ttgatgtaga tatccctttt gaagatccta tacaatagaa agaaggtaaa ataatgatca
    45721 acttaggtat tatcggaaca aattggatta ctcatcaatt tgtccaagca gctttaagca
    45781 caaaaaaata tgaattgaca gcggtatatt ctagacattt ggaaaaagca caggaatttg
    45841 gttctcatta tgaaggggat atcgaattcg caacagattt gaagactttt ttcaatatta
    45901 ctcatatgga caccatttat attgcttctc cgaatagtct tcactttagt caagctaagc
    45961 aagcaatttt ggcaggaaaa aatgtgatag ttgaaaagcc agctttttcg acgccaaaag
    46021 aaatggatga aatcatccat ttggcaaatg aacacaatgt gtattttttt gaagcagcaa
    46081 gaaatatcca tgaatcaggt tttaaaaaga ttgccgatta cttaccatta aaagacgaga
    46141 taatcggtgc aaatttcagc tatatgaaat attcttcgcg ctatgaccaa gtattagatg
    46201 gagaagaacc gaatatcttc tcgactcatt tctcaggtgg tgcattgatg gatttaggtg
    46261 tttaccttgt atatgcagct gttgcttggt ttggaatgcc aaaagaagtc cattatttcc
    46321 caagaaagat ttctactggt gtagatggtc ttggatgggg gatcttacgt tatgagaatt
    46381 ttgatgtatc gatccagcca ggtaaaatcg gtgattctta tttaccgtca gagatttatt
    46441 ttgactcagg aacgctagtg atgaatggag tcaatgcaat tgaacaagca caatttcatg
    46501 atcgcgcgca taaagaaatc attgatttgg cggtaacagc tgcagaaaat ccaatggcag
    46561 atgaagcaga ggattttgcc aatgtcatgg ctcatccgaa agatccagct tggggattgt
    46621 tgtatgaaga gtggatcgag ctagctagaa atgtgaacca agtgatttat gatttaagaa
    46681 aaaatggcgg catcaaattc gatgcagata atgaggaaga tttttaatga acgaatttgt
    46741 acaagtattt cccgaaaatt ggcagaaaaa atatgaggat aaagggttta tctcaccttc
    46801 tcttattcaa cgagcagttt ttcagccact aagtaataaa caaagtatag tagcggtttc
    46861 tcctacaggt tcaggtaaaa cactggctta tctttggcct ctactactaa acgtggaacc
    46921 aggtgaagca agtgcgttgg tcatttttgc ttcttctcaa gaactagcta ttcaagtagc
    46981 agatgttgca cgtgaatggg gaaaagacaa agagttgaaa gtccaatctt tggtgggcgg
    47041 agccaacgtc aaacgtcaga ttgagggttt gaaggaaaaa ccagaaatct tgattggtac
    47101 accaggaaga atattggaat tgatgaaagc aaaaaaaata aaagcacatc aagtgaaaac
    47161 aatggtattt gatgaagcag accagctatt tgacgctggg aacagccaaa tcattgatca
    47221 aattcttcat caagctccaa cagagtatca gctagctttt ttttcagcga cagcagatcg
    47281 ttcccttgaa agcattgaaa aaatcactgg gaaaacgttt gagacaattg acgtgacagc
    47341 agaggacgat tctcgaaaag gactgagaca ttacttttta cgtgtaccga tacgaaagaa
    47401 agatgactac ttgagacgtc tcacgcatat agaagatttt catggtctga ttttctttaa
    47461 tcaactgact gaattaggcg taatggaaga aaaattgctg tatagaggtg taccggtagg
    47521 aagtctggcc tctgatcaga acaagctact ccgaaaagca gcgctagaac aattcaaaaa
    47581 gaaaatggtc agagcacttt tgaccacaga tgtggcggct agaggtttag atattacagg
    47641 tcttccttat gtagtgaatg cagaagttcc gttaagtgaa gaagcttatt tgcatcgttc
    47701 gggaagaact ggtcgaatgg gtaatgaagg aatagtaatt acactcgtac aagataataa
    47761 tctaagagat ttaaaacggc ttttacgtcc atcaaatata acacttgaag agattttttt
    47821 atacggaggt aagctgcaaa ctgagcagcc aaccaaagaa gatgcagctc ctgcagtaaa
    47881 taaaaagtat gcgaaaactt tttctggaaa ggagcagaac ccagaatata acaaagaaaa
    47941 aataagaaaa aaagttaaaa ataaatcaaa aaaagagaga aataaaggag caaggcgcaa
    48001 ataagcgcct ttttatgcta tgattatgaa aacgggaaca aaattggttg ccagaggagg
    48061 ggttatgtga aagaacgtca agaagagatc ttgcagctat tgacagaaaa ccaacaagga
    48121 ttgacagcaa gtgatgtagc tgagcgctta actatcgatc gaagcaatgc aagccgatat
    48181 ttaagtgaac tatacaaggc ccatcatatt gtaaaaaccg ctggccgacc agtcgtttac
    48241 tctttgccaa cggaaaagtc aaaatctgat gaagtgcatg tcgactcttc tacgcaagtg
    48301 acttttgaaa cactcgttgg agaaaatgat tctttaaaag tcagcataca gcaggcaaaa
    48361 gctgcgattc tttatccacc gcgggggttg catacgatta tttttggaga aactggtaca
    48421 gggaagtcga tgtttgctga atgtatgtat cattttgcaa tcgattcaga gatgctttct
    48481 gcagatgcgc catttgtttc tttcaattgt gcggattatg ctcaaaatcc gcaactgctc
    48541 tttggacaca tctttgggat caaaaaaggg gcttacacag gagcagctca agatagtccc
    48601 ggtctaatag ccaaagcaga cggcggtata ttgtttttag atgagatcca ccgtttaccg
    48661 ccggaagggc aggaaatgct gtttactttc attgataaag gtatttaccg tccgcttgga
    48721 gaaagtgcac aagtacatga agcatctgtt cagatcattg gggcgacgac tgaatcttca
    48781 gaaagtttct tatctacgtt caatcgacgg attcctatgg caatcacctt gccgaatctt
    48841 gcttcacgtt cactggatga gcgttatgaa atcatctctc tttttatcaa acaagaagcg
    48901 aatcgtttga atcaacgtat cgatatcgaa aaagaagcga ttcttgcctt catgctttat
    48961 gatgcggaag gaaatatcgg tcaagtgaaa cgagacttga aactagtctg tgcaaaatct
    49021 tttttacatt accgtactca tcatgagaaa aagctaatca ttcgaaaaga agaactttcc
    49081 cttcaagtac aaaaaggatt actgaaaatc aaggaaatac cggaacgtct cgaccgattc
    49141 atggacagca aaagtcagta tttgacattt gaaccaggat ttgctgatgt tgtctggtca
    49201 caagatccag aacgaaacat gcaggtctat aacgatattg aggaaaaagt cacttctctt
    49261 tccgaaaccg gtgtggagaa cattgatcta gagaccctca tttcaaaaga tgtggatgct
    49321 tatttccaga catatgtcaa agaattgact aaaagttcga ttcccaaaga tcttttgcca
    49381 gatgaaatct ggcggttaac caatcaattg tatgatgtgg ctgaaaaacg gttagaccgt
    49441 atttataatg aaaaagctcg ttttgctttt gctttgcatc tgcaaagtac attggatcgt
    49501 gtgagagaag gtcatatgat cgtccaccca gatttgaata acgtacggaa aaattttaag
    49561 agggaattcc aggtagcaat cgatctttca acgatcattg aagaagaaga acaagttgag
    49621 attccttttg atgaaatcgg ctttattagt atgtttcttt ctatcaatct aggagaaagt
    49681 gagcctgtta aagaaaacaa agtagatgtt ctcatattga tgcacggcag acagactgct
    49741 tcaagcatgt tggagatggc acaagaacta ttaggaacaa aagtagggaa agcttttaat
    49801 atgccactgg ttatggaagt tcaaacaatg tatgaagaag tgttacaata tgtcaaaaac
    49861 tatcaagagc atttgacgaa tggcctactc ttgttaacag atatgggatc attaaacacc
    49921 ttcagtgatt tgatttatga agagactggc attcggacga aagctatcac gatgacaagt
    49981 accatgattg tgatagaagc gatacggatg gcaagtgttg gaagaagctt agaagatatt
    50041 tatcaaaata tccagctttc ctttgaatcg atcgtacgtg atcagttcaa agcggaaaag
    50101 tctgaaactt tacagcgtaa aaaagccgta atcgtgacat gctttactgg tgaaggagta
    50161 gctgccaaat tatatcagcg tattatgcca gtgattaatc aaaataaggt agaactgatc
    50221 cagatgcagt ttttagaacg tgaatcattc aaaaaacaca tcgatgcctt attggaagaa
    50281 tatgagatca aagcaattgc gggaacagtt gatgttgatt atcaaaatat cccatttttt
    50341 tcagcctatg acgtttttga tgatgaaaaa ctaaatattt taaagcgtat agctgatgat
    50401 gagatcccga cagctaaaat tgtcgattca cttgctggat cattaagcga tgtcttttct
    50461 attgatgaac tgatccgttc attgcaaaag acagttcagc agatccagac cgatttgtcg
    50521 attatcgtgg aacctggagt agatactgga atcgttatcc atttagcctt catgttagac
    50581 ggattgatca aacaagaaag ccatcggcaa tttgagaaac tgtctatttt ctcaaaaatt
    50641 tatcgtttgg aaatggatat cgttcgggca aacttgatgg cactagaacg aaaatatcag
    50701 gtaaaaattc cagaagatga tatcgcattt cttacacaaa tgtttcttga aaataaaata
    50761 aaaaaatgaa cacactaatc atactgttta cacactaaac atctgtgtgc gcactataaa
    50821 aaagagctaa aaagcatgaa aacgctattt cagctctttt ttttattggc atgcttcttg
    50881 cgttatataa aagtgtaaaa aaataaaagg agtgttaaaa atggcaaaaa aaacaatcat
    50941 gttagtttgt tcagcaggga tgagtacgag tttattggtc acaaaaatgc aaaaagcagc
    51001 agaagaaaaa gggatggaag ccgatatttt tgcggtctct gcctcagatg cagataataa
    51061 tttagaatct aagaatgtcg atgtattatt attaggtcct caagtacgtt tcatgaaagc
    51121 tcaatttgaa caaaaacttg cgcctaaagg tattccatta gatgtcatca atatgcaaga
    51181 ctatggcatg atgaacggag aaaaagttct tgcacaagca gaaaatctaa tgaaataaat
    51241 caagtatgca gttgggggta taacaacgtg gaagatcaac aaaatctaga agccatcatg
    51301 gggttaatca tgtttggcgg taatgcaaag agcgatgcaa tggaagcgat tgcggctgct
    51361 aaaatcggtg attttgagtt agcggatagg aaaatcgctg atgcagaaga atcacttata
    51421 caagcgcatc atgcacaaac agacatgttg acacaagaag cacaaggaaa tcatatgcaa
    51481 gtgacattat tgactgttca cagtcaagat cacttaatga catctattgc gtttactgat
    51541 ttagcaaaag aaatcatcga actttatcgt cgcgtataca gcgaataaat acaacttctt
    51601 ttttgaacgg aagaagaaaa atttttgaac ggatttttta ttttgttata aggagaactc
    51661 tctttatata aaaaacttat aggaggaagt aaaaaatgaa cggactatca agttggatgg
    51721 agaaatacat cctgccggtt gccgcaaaaa tcggagcgca aaaacattta gttgctttgc
    51781 gtgatgcttt tatcggcaca ctgcccgcaa caatggcggg atcagtcgct gtaatgatca
    51841 atgcaattat tcgtgattta ccgcaacagt ttaacagtac ctatgctgac agtttagcag
    51901 cagacaaagg aatctttgcc gtattgaatg tcatcatcgg aatcaatggt tttgtctgga
    51961 acggtacact agcaatcgcc ggattgatct ttgccttttc ttgggggtat aatcttgccc
    52021 gagcttatgg agtaaatgat ctagctgggg ggatcgttgg tttagctacc ttgattcaag
    52081 gtatttcctt tggtccagca ttatcagcag cattagatgt gaatgttcct tcaaatgtcg
    52141 cagatgcaat caatagttct gagattggtg caacgattgc agatggcaca ctgtcagttg
    52201 gattatgggg ttggctaaaa ctagatcatt tgaatggaaa cgcttatttt acagttatga
    52261 tcatgggagc tatttcagtc attatttttt gtaaattgat gcttgccaac attacgatca
    52321 aaatgcctga ttcagtacca ccagcagtat ctaaagcatt tgcagccatt ttaccagcga
    52381 cgatcgcttt atacgtcatt gctattatca attatatcgt aggtcgtgta gctgatgggc
    52441 aactgatcat tgatttggtg caaaaatata ttgcagagcc attcctagga ttatctcaag
    52501 ggttgggagc agtattgatc gtcacagtat ttgttcaagt attctggttc tttggtattc
    52561 atggaccaaa tgtattggca ccagtgctag agggaatctg gggacaagca caactaatca
    52621 atattgatat tttccaaaaa ggttataacg gaaaaactgg tacaccagcc gtgttagatg
    52681 caatcgaaga tggaaaagct tatatgtggg tccgcggatc gttcgatgct tttgcatggt
    52741 ttggcggatc aggcggaacg atcgtcttac tgatagctat cttgattttc tctaaacgtg
    52801 cagattatct aacagtagct aaattatcca ttgggcctgg tatcttcaat atcaacgaac
    52861 cagtgatgtt tggtctgcca atcgtattga atgcaatcat gtttatccca ttcttgattg
    52921 ctccagtagt agcaacagca attggatgga cagctactta tcttggccta gtcgctccgg
    52981 tatcgcagca agtcacatgg gtagtaccgc cgttcttatt gtcattctta gcaacaggag
    53041 cggattggcg ggcaccgatc gtaacattag tatgtatggt cgtgactttc ttgatttggg
    53101 caccatttgt catctctgca aacaaaatgg accctgccgc aggcgaacaa caataagatg
    53161 aacgtgagga gcgggacgct ttaagtttcg ttcctcataa tgaattttcg aggaggaact
    53221 gaaatgatta caattgccag tgcaatgcaa caagatgaaa tcaaagaatt gttagaagga
    53281 tacaatgaag aaggaatgac ttttacattt aaagaaaaac aaggaatcaa gctgttcttt
    53341 gaaacaagtg cagaagatcc ggaagttgcg gctaaaaaag caaaggaact aatcaaagca
    53401 cagccttggg gaacagtcct ttattttcaa tcaacagctg tttagaaaat ggtttaaaaa
    53461 gataggatgg ttgagatgat aaaaaacggt ttatttttat gtagtattgg ttctttgatc
    53521 gtgttgtata gtttgaatcc acattcgcaa aggtatgatt tgtatagtat ggtcacaggt
    53581 ctttttctaa caggattagg aatctatttc ttcatgaagg ggaaaaaaag agaagaagca
    53641 aaggagaaaa ataactaatg gaaatcacga aaaaattaaa tattccagca tcgttttttt
    53701 acgataaagt tatggactct gtccttttcg atgtacgtaa agcgaccggc aaatcagtaa
    53761 ctagaaaaca gctgaaaaat ttggagtacg tcaaacaatt ctcgcaaaat aaccgggctc
    53821 gcataaaaat cgaagaagtc attgaaaacc agtcttataa gtttcgtaca tcgacaacga
    53881 agaatgaatt cgtcgttcat tatcaaatca aaccattgga cgattcgacc tgtgaggttc
    53941 attacacaga aaaaatggag tcttatggca tgcttcaaaa gttgaatgat atgcttttag
    54001 gaacaatctt gatgtatttt aaacggagac gtttcaaaaa aatgctagat atgatcgaag
    54061 caacgtatta aatcaatgaa acatggtact atttagttat tgaaaaatga agtaagctag
    54121 aaggaatttt tcataggaag atagatcttt cctgcgaatc ctcactagct tttttgtagg
    54181 aaggggcagc aaaatgacaa acaaagtaag agagctaggt gtatcttcga ttgaggaaat
    54241 gtttgattta gccacttatg catttaatgg ggcagataca cctgaaaggc gagaacgttt
    54301 tcgtacactt gcggagaatt cttggaatta cggatttttt gatgaggagg atcggctaac
    54361 aagtcaagtc atggcgaccc cttttccagt gaatttttac ggacaagagt acctaatggc
    54421 cggcattggc tatgttgctt cttatccaga agcaagggga caaggcggga tcaatcagat
    54481 catggaaaag atccttgaag attgccgtga tcgtaaagta agcttatctt atctggcgcc
    54541 attctcctat cccttctatc ggcgctatgg ctatgaacaa tcatttgata aaatcagtta
    54601 tcaactaaac agtcgtgatg tgccgtttat caaaaaaaaa tctggaaaaa taaagcgtat
    54661 ggattttgaa gatgcaaaag ctgcaatcag aagaatttat gaacagatgc cggaaaacca
    54721 acgagctggt ctgaagcgcg aagactggtg gtatacgtat aaattcaagc aaaaaggcaa
    54781 ttaccagttt gctttgcatt ttgatgataa tgaggaagca acgggatata tcgtttatca
    54841 gcttgcaacg tcttccttta ttattgcgga gtggggattt ttggatcatg atgctttttg
    54901 cagccttgtt cgcttcgtaa gttctcacaa tggtgctttt gaaacatttg aatattcatg
    54961 cggctacggc ggtaatgatc aaaattatct attagaaaat ccatttgctt ctgtaatgat
    55021 cacaccttat atgatggcga ggatcgtaga tatttctttg tttttggaaa attatccatt
    55081 taaaaagaga caagctgcat ttgccctaca aatagaagaa gatcattatg cgaaatggaa
    55141 tgaaggaatc gtggaagtaa caatcgaaaa tggaaaaaat catctctcaa aagtagaaca
    55201 aacaaaactc ccaatcgttc gaggaagtat ccaacatatc acgcagctat tattagggta
    55261 tcgtaaagtg gaagagttgg tattccaaga aagaatacag gtgacaaaag aatatgaaga
    55321 aacgttaagc tcaatcttgc cacagcaaca gccaattctt aatgactatt tttgataaaa
    55381 aacgggtgat gacaatattt ttgtcaccac ccttttgttt ttcatttggc atttgaagga
    55441 aaatgttaaa gtaaatcctc ttttttcgta aatttgaatt ttttgttttc ttttgtttta
    55501 gggttttctt ttttgtggtc ttggattgct tctttcgtaa tcttttcccg atttttttta
    55561 gatgaatcta agcgttctgc gtatactttt tcttttggct tatcattcat tggaaaggac
    55621 ctccctacat tatttcattc tacctctagt ttatcatctg ttaaggacta gtggaaacgt
    55681 tttgcctaac ttataagctg atttttttag cagataagct tgcaagtaaa aaacaaacaa
    55741 gctacaattg atttattgaa attgttcctg tagctcagta ggatagagca atcgcctcct
    55801 aagcgatagg tcgctggttc aattccagtc aggaacgtac aaataacccc tgtatccttt
    55861 gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg ctatgtccta
    55921 aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat gcatgcgtat
    55981 gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt actaaagaaa
    56041 aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga attaaacgat
    56101 ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa ctctagcggt
    56161 ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt tttattattt
    56221 ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc atataaaaaa
    56281 acggtggggt tgtttagcga aataaaggat ctaagccaat agcttagatc ctttattcta
    56341 tgctttatat tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg
    56401 aaaatcggta ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct
    56461 accatgaaat ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg
    56521 gacatttctg taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc
    56581 gatgcgtttt catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa
    56641 gggtcaggta caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt
    56701 tttgctaaga catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa
    56761 gtaaatttca tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg
    56821 taaccatcta ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc
    56881 atgaattttt ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc
    56941 aatccgataa tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga
    57001 ttcaattctt ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg
    57061 tcttcttcat agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt
    57121 tcttctggca gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca
    57181 gtccaactga ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc
    57241 gctgattgat aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca
    57301 gaaacaacgc tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta
    57361 gattgtggta cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc
    57421 tggtcgtcag tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg
    57481 atcaatcgag caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt
    57541 tctgtggcta cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa
    57601 tcagataaca taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac
    57661 catttaatta tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag
    57721 gttaagtttc atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac
    57781 taattttgca tatcaaaaaa acatgttaca atgaaattaa tcaatggtgg aagcgttata
    57841 aaaaaattaa ggagattaaa aaatatggca agaaaaaaga cgattactag agaacagatt
    57901 ttggcagctg cttatgaagt agctgccacc gatggattta cacgatttac tgcgagaaac
    57961 atcgcgaaca aaatgaattg ttccacgcaa ccgatttatc ttgagtttaa aaatatggat
    58021 gacttgagac aagctctttt tgagcagatc tatgatcatt tacaatatga agtgttcact
    58081 gttgagcata cagggaatac gattatcgat cttgctttta attatatcca ttttgcaaga
    58141 agagagaaga agttgtatcg ttcactgtac ttagaagaat atggtggagg aaaagagatg
    58201 cagaatttct cttatactta ctttgtgaat acagtgaaac gcgatccagc ttatgtgaat
    58261 ctttcagaag agcagatcga atctttgtat aatgggacat ggatcatagc tactgggatc
    58321 gcagctttga tgtcttcaag catcatccat ccaactgatg aacagattga aaagatgatc
    58381 caagacagta tagatgctat cttgaaattc aaagaaccaa tcgaaatcga ttgaatatac
    58441 tcaaaagttc cacaaatcag taggtaacaa gagatgtgtc aaaataagga aaagttcgtt
    58501 tttcttacag cgtatgcatt tttaactccc gaagaactct tccatgaatg caatgaataa
    58561 aagagccata cagattgaat aaatctggat ggctctttta ttgcaagtat attaaatttt
    58621 tgcgactagt tctttagcga aagcttctaa attttcgata tcttcttctt cagcattcag
    58681 atccacttta acattttcgg ctccttttga tgcgcctgtt tttgcaaaag cagcatcgaa
    58741 atcatccacc gatttgcaga attcatcgta aaaagtatca ccagatccgc agacaccgta
    58801 aatttttccc aatagatcca attcttgcaa gtcttcataa aagtctacga tctcatcagg
    58861 cagatcgcca tcatcgtatg tataagtagc tactacgcaa atatctgcct cctcgaattc
    58921 atctgcatct acttgggtac attcgttgat ttcgacttcg atatccagat tttcaaatgc
    58981 ttctgcaaca atatcggcga tttcttcagt gtttcctgtc atgctagcat atacgatttt
    59041 tgctaaagcc atgatgtgcc tcctcgttta taattattta acaattatga attataccaa
    59101 aacatcattg aaaagaaaat aaaacctaaa gacacctggg gaatgtcatt tggctaaatg
    59161 tatgccgcta tgttaaaatg agagggattt gtaaaaagga gacgaaaata tgattacttt
    59221 aaaatcagca cgtgaaattg aaatgatggc agaatctgga gctttattag cagatgtaca
    59281 taaaaatctg cgtgatttta taaaaccagg aatcacaagt tgggatattg aggtatttgt
    59341 cagaaactat atcgagagtc atggcggaat tgctgcgcaa attggctttg aagggtatga
    59401 atatgcgact tgtatcagta taaatgatga aatttgtcat ggattccctc gtaaaaagcc
    59461 tttgaaaaac ggtgatttgg taaaagtcga tatgtgtatc gatttgaaag gcggcatttc
    59521 tgattcttgt tgggcctatg tagttggaga atctacacct gagatcgacc gattgatgga
    59581 agtaacgaag aaagctttgt acatcggtat tgagcaagca caagtaggga atcggattgg
    59641 tgacatcggt catgctatcc aaacttatgt agaaggcgaa aatctagcga tcgttcgtga
    59701 ttttattggt catggcgttg gtcctacgat tcatgaagaa ccagcagttc ctcattacgg
    59761 agaagctgga aaagggttgc ggttgaaaga aggcatggtt atcacgatcg aacctatggt
    59821 taataccgga acttggaaaa tgaaaatgga tcctaacggc tggactgcgt atacaagaga
    59881 cggcggactt tcttgccaat atgaacatac attggctatt actaaagatg gtccaagaat
    59941 tttgacatca caaggagaag aaggcactta ttgatctatc cagtcaatag gtacgggaga
    60001 ggtacatgaa agtttggaac aaaataaaac aaaatgaaaa tctgatgcgc tttattgaga
    60061 cgactcaaaa gcgcatcact gattcagaaa tgagtacgac ctctgtcgtc gttgcttatt
    60121 atttactctt gtctttattt ccattgatta ttgcttttgg taatatttta ccgtttctcc
    60181 acatcgatca agagactgta ctgacctatt tacgagaagt gattcctgag accgtttacc
    60241 agtttatcgg tcctgcaatc aaagaccttt tgacacaaag ttcaggaggc ttgttatccg
    60301 tttctgcatt agcggcttta tggtctgcaa gtcaaagtat caatgctttg caaattgcga
    60361 tgaataaagc gtatggcgta gaaaatagaa aaaatttttt gatcgtccgt ttcttctcac
    60421 taattgtcat tgtcttgttc atgattgcta ttagtggggt aactatcgtt cttgggttgg
    60481 gacagctgat tttggatgct attcagccaa tcgtacaaat acccgtgaac tttatcgatc
    60541 agttccaaac tttgaagtgg ccaatcacat tggtagcttt atttgtcatc atgttcttga
    60601 tttatttaat tgtccctaat gctcagttga agttgaagac gatcattccg ggcacgatct
    60661 tcgctactgt cggctggatg ctgttatcac aagtgtttag tatctatgct aggtattttg
    60721 caacgaggat cagcggttat cagatcatag gaagttttat tgtactcatg ctttggctta
    60781 attttgctgc tacgatcatc attttgggcg gaatcatcaa tgcagttgtt caagaatacg
    60841 tgacgggtca tcaaatcaaa aaacgtcgag atccaagccg aaaatggttc aataaattga
    60901 aaactaaatt ttctcataaa aaaaagtaga
//
LOCUS       DARDHA010000002        58876 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000002 DARDHA010000000
VERSION     DARDHA010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.002"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..259)
                     /locus_tag="SDA10_000068"
     CDS             complement(<1..259)
                     /locus_tag="SDA10_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239003.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFS"
     gene            complement(434..2569)
                     /locus_tag="SDA10_000069"
     CDS             complement(434..2569)
                     /locus_tag="SDA10_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4239004.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(2652..4307)
                     /locus_tag="SDA10_000070"
     CDS             complement(2652..4307)
                     /locus_tag="SDA10_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG4239005.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(4309..4623)
                     /locus_tag="SDA10_000071"
     CDS             complement(4309..4623)
                     /locus_tag="SDA10_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG4239006.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            4793..5053
                     /locus_tag="SDA10_000072"
     CDS             4793..5053
                     /locus_tag="SDA10_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEG4239007.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            5067..5381
                     /locus_tag="SDA10_000073"
     CDS             5067..5381
                     /locus_tag="SDA10_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEG4239008.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(6111..6665)
                     /locus_tag="SDA10_000074"
     CDS             complement(6111..6665)
                     /locus_tag="SDA10_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEG4239009.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(6791..8719)
                     /locus_tag="SDA10_000075"
     CDS             complement(6791..8719)
                     /locus_tag="SDA10_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEG4239010.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(9025..9741)
                     /locus_tag="SDA10_000076"
     CDS             complement(9025..9741)
                     /locus_tag="SDA10_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEG4239011.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(9742..11565)
                     /gene="glpO"
                     /locus_tag="SDA10_000077"
     CDS             complement(9742..11565)
                     /gene="glpO"
                     /locus_tag="SDA10_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEG4239012.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(11579..13075)
                     /gene="glpK"
                     /locus_tag="SDA10_000078"
     CDS             complement(11579..13075)
                     /gene="glpK"
                     /locus_tag="SDA10_000078"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEG4239013.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(13211..14671)
                     /locus_tag="SDA10_000079"
     CDS             complement(13211..14671)
                     /locus_tag="SDA10_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4239014.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(14661..15956)
                     /locus_tag="SDA10_000080"
     CDS             complement(14661..15956)
                     /locus_tag="SDA10_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG4239015.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(16288..16965)
                     /locus_tag="SDA10_000081"
     CDS             complement(16288..16965)
                     /locus_tag="SDA10_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEG4239016.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(16995..18086)
                     /locus_tag="SDA10_000082"
     CDS             complement(16995..18086)
                     /locus_tag="SDA10_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEG4239017.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(18187..18621)
                     /locus_tag="SDA10_000083"
     CDS             complement(18187..18621)
                     /locus_tag="SDA10_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG4239018.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(19205..20662)
                     /gene="ascB"
                     /locus_tag="SDA10_000084"
     CDS             complement(19205..20662)
                     /gene="ascB"
                     /locus_tag="SDA10_000084"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG4239019.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(20659..22554)
                     /locus_tag="SDA10_000085"
     CDS             complement(20659..22554)
                     /locus_tag="SDA10_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4239020.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(22612..24387)
                     /locus_tag="SDA10_000086"
     CDS             complement(22612..24387)
                     /locus_tag="SDA10_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEG4239021.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(24437..25933)
                     /locus_tag="SDA10_000087"
     CDS             complement(24437..25933)
                     /locus_tag="SDA10_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG4239022.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(26127..27749)
                     /locus_tag="SDA10_000088"
     CDS             complement(26127..27749)
                     /locus_tag="SDA10_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEG4239023.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(28198..29511)
                     /locus_tag="SDA10_000089"
     CDS             complement(28198..29511)
                     /locus_tag="SDA10_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEG4239024.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(29756..29980)
                     /locus_tag="SDA10_000090"
     CDS             complement(29756..29980)
                     /locus_tag="SDA10_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239025.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(30000..30422)
                     /locus_tag="SDA10_000091"
     CDS             complement(30000..30422)
                     /locus_tag="SDA10_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEG4239026.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(30566..31276)
                     /locus_tag="SDA10_000092"
     CDS             complement(30566..31276)
                     /locus_tag="SDA10_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239027.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(31294..31791)
                     /locus_tag="SDA10_000093"
     CDS             complement(31294..31791)
                     /locus_tag="SDA10_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4239028.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(31828..32280)
                     /locus_tag="SDA10_000094"
     CDS             complement(31828..32280)
                     /locus_tag="SDA10_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG4239029.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(32415..33020)
                     /locus_tag="SDA10_000095"
     CDS             complement(32415..33020)
                     /locus_tag="SDA10_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG4239030.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(33470..34090)
                     /locus_tag="SDA10_000096"
     CDS             complement(33470..34090)
                     /locus_tag="SDA10_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4239031.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(34092..34961)
                     /locus_tag="SDA10_000097"
     CDS             complement(34092..34961)
                     /locus_tag="SDA10_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HEG4239032.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(34984..35391)
                     /locus_tag="SDA10_000098"
     CDS             complement(34984..35391)
                     /locus_tag="SDA10_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEG4239033.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(35410..36699)
                     /locus_tag="SDA10_000099"
     CDS             complement(35410..36699)
                     /locus_tag="SDA10_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4239034.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(36726..37028)
                     /locus_tag="SDA10_000100"
     CDS             complement(36726..37028)
                     /locus_tag="SDA10_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4239035.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(37053..39146)
                     /locus_tag="SDA10_000101"
     CDS             complement(37053..39146)
                     /locus_tag="SDA10_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG4239036.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(39635..40399)
                     /locus_tag="SDA10_000102"
     CDS             complement(39635..40399)
                     /locus_tag="SDA10_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEG4239037.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(40510..41379)
                     /gene="mdcB"
                     /locus_tag="SDA10_000103"
     CDS             complement(40510..41379)
                     /gene="mdcB"
                     /locus_tag="SDA10_000103"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HEG4239038.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(41404..42480)
                     /locus_tag="SDA10_000104"
     CDS             complement(41404..42480)
                     /locus_tag="SDA10_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEG4239039.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(42504..43097)
                     /locus_tag="SDA10_000105"
     CDS             complement(42504..43097)
                     /locus_tag="SDA10_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HEG4239040.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(43101..44777)
                     /gene="mdcD"
                     /locus_tag="SDA10_000106"
     CDS             complement(43101..44777)
                     /gene="mdcD"
                     /locus_tag="SDA10_000106"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEG4239041.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(44767..45066)
                     /locus_tag="SDA10_000107"
     CDS             complement(44767..45066)
                     /locus_tag="SDA10_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HEG4239042.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(45168..46814)
                     /gene="mdcA"
                     /locus_tag="SDA10_000108"
     CDS             complement(45168..46814)
                     /gene="mdcA"
                     /locus_tag="SDA10_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEG4239043.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(46821..47732)
                     /locus_tag="SDA10_000109"
     CDS             complement(46821..47732)
                     /locus_tag="SDA10_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEG4239044.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            47852..48709
                     /locus_tag="SDA10_000110"
     CDS             47852..48709
                     /locus_tag="SDA10_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG4239045.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(48896..49258)
                     /locus_tag="SDA10_000111"
     CDS             complement(48896..49258)
                     /locus_tag="SDA10_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEG4239046.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            49604..51043
                     /locus_tag="SDA10_000112"
     CDS             49604..51043
                     /locus_tag="SDA10_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4239047.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(51050..53602)
                     /locus_tag="SDA10_000113"
     CDS             complement(51050..53602)
                     /locus_tag="SDA10_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEG4239048.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(53716..55047)
                     /locus_tag="SDA10_000114"
     CDS             complement(53716..55047)
                     /locus_tag="SDA10_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4239049.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(55013..56491)
                     /locus_tag="SDA10_000115"
     CDS             complement(55013..56491)
                     /locus_tag="SDA10_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4239050.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(56796..58631)
                     /gene="tdc"
                     /locus_tag="SDA10_000116"
     CDS             complement(56796..58631)
                     /gene="tdc"
                     /locus_tag="SDA10_000116"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEG4239051.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT        16914 a        12593 c         9914 g        19455 t
ORIGIN      
        1 aactgaaaaa aatcaataca aaaaagccga ttaagaaata cagcattttc ttttctgata
       61 aaaaagcgta caaggaaaaa tccttggctc tttctggtct cgttaatggg aaaaagaccc
      121 atataaaagg aagaacaacg atcatccatc ctaaaagagc cactaacgtt tgccaaatct
      181 ttaaccagta attacctttt gaaaaatact tatctttcac caaaattgat gcaacttctt
      241 ctcttgttac ttttttcata tcctcacctc acgttttttt gttacgacgt aggagtttac
      301 catacatttc atttcttttc acataaagtg ttcctaaatt tttcctaaga aaaagaacaa
      361 aacatttctc atcagcaaat gaaaaatatt ttgttctttt tatcagttgt tctttttatt
      421 ttttatctct taattatgtt gctctttcca atacgacaat gcttttcatt cctctagata
      481 tcgccgtgta catttgtctg cgtcttcttt gctcatctcc atcgttttca aatggatata
      541 agattacatg atcaaattcc agtccttttg ctagactcaa aggaatgatt tggcagtttt
      601 ctaatccata tgaatttttt ttaatcgatt cttgtagcgt ttgggtttcc tcttctgttt
      661 gggtgataac tgccgctttt tctcctttta gagaaaccaa aatctcagct agtattttca
      721 acaattcttc agaggatcga aaagagcggt attcgggttc ttttccttgc ggtcggatcg
      781 atacgatgtc gatcttctct tgatccacag cataggtttt gaaaagctct gtgattgctc
      841 cgctagaacg gtaactattt ctaagatcat accgatggat cggcagatgg tagtcgtcga
      901 aacaacgcat aatatcagca aaagtagtgg aagaattaaa gattgcctga ttctcgtcac
      961 ctactaaagt aaagtgagtt tttggaaata attcgatcaa tagacctaat tgtgccaggg
     1021 tataatcttg tacttcatca atcaatagat agcgaaatgc tggtacattt accttttcaa
     1081 caagtaaatg acgaatagct aatataatga ctgcttcatc aacagtacgt gtgggttgcc
     1141 aagcataagc tgagccatat cgtttttcat aaattctttc aaacaactga tgctcatcca
     1201 cccatgccat ttcttcgatt tgtctggtta tttttcgata ttttttttgt aaaagtttcc
     1261 ttgcataagc tacaatactt tgttccgaat catcagaaat caatttaccg aataattcca
     1321 actgcctatc ttctgtcaac gagagtacct gattctgcac agcaggactt tttgcttgtt
     1381 tcaaaagatg attcttccat tcgctcatca gtgccttttt cgttgcttga agcctatcga
     1441 tcaatttagg atgagcagga actttctcat atattttttc gatcgttttt ttagaaagga
     1501 ctgtttttcc atttttagta agtccacgga taaaattcgg atgatctaaa aacaaaggat
     1561 ctgattgttt tagattatcg ataaactttt tgctacgcaa acgttctgtc tgctccgata
     1621 cattttctcc cgtgatacgt ttgaaataag cctcttcacc ttctatttcc tctgctgaaa
     1681 gctgtgaaac cagctgtatg atggttagat tcagtggatt tctttcacct aatgatggta
     1741 ggacattcga aatatagtcg ataaaccgat gattaggtga tagaatcagc aagtcatcgg
     1801 atgtcatcgt atctcgatat aaatagagca gatacgcaat acgctgcatg atagcagagg
     1861 ttttaccact tcctgctacg ccatttacca agatattttg acttttcaca tcccgaatga
     1921 ttgtgttttg ttccgattgg atcgtagcag tgatagcctt catttcattg gtctcatctt
     1981 ggcctaagac ttctaataat acgtcatctt gaatagctac agacgtatca aaaaacttga
     2041 ccaaacgatc tctttccaaa agaaattgtc gtctttcttt aataaccgta tcgattttat
     2101 tttgacgaac cgtataagaa gatggaccaa tcatgttatt gtaaaaaagt tcggcaattg
     2161 gtgaacgcca atcataaaca agattttcat ctgcatcatc tgtgaaattt gctgtaccga
     2221 tataaaagga ttcttttggc tcattatcca gaaagtctac aacgactttt ccaaagtagg
     2281 gacttttcaa taaacgttcg atctttgcta gctgattttc tgcgtttttc tgtttgatat
     2341 tcatctgatc gatttctcta tttttcattt caatcatcga aaaagtatcc aggttatcta
     2401 agtaactgtc aaaattcaat cgaagctcgc cagccatttc tttcaacgac tggtttcctt
     2461 ctatttttgc cgtatccatc gattcccgca gttcgttttt tttatcaatt agcttttgat
     2521 acacgttctt cagatatgct tcttctaact gtttttgtgt gttgaccata cacattctcc
     2581 tcttttttta aagtggtgga accgattagt ttacctcttt taagagtcga atgcaagctt
     2641 tttaacgagt atcatttgtt aggatatatc agttcttcag gtcggacagt ttgatataaa
     2701 gaaaatgcac catcaaggtt ttttacgtgg tagccagact gcttcaagat tctttccgct
     2761 agatagctac gtaatccgct atgacagctg acgatatatt cttgttggct gtctaattca
     2821 tttaatcggt cccgaagttc attcaaaggg atatgtttgg cttccttgaa tttgcccttt
     2881 tccaattctt ctgctgttcg cacatctaat aaaatttttc cttttgctag ttctgtcggc
     2941 aattcatgcc attgtacggt ttcactaagc ccttctgcaa tgttcattgc tgcatatcct
     3001 agcatgttta ctggatcttt ggctgagcca aatggcggtg catacgtcaa ttctaattcc
     3061 ggtaaatcga agatcgtcaa atgccctttg attgctgttg ctaaaatatc gatccgttta
     3121 tccacacctt ttgccccaac accttgtgca ccataaatct cgccagtctc tggatggaag
     3181 atcaacttca gtaaaatatc tgttgcttct ggataatagc ttgcatgatc ttttcctgat
     3241 acatgaatga cttcaaaaga cagttcgttt tctttggctg ttctttcact taaaccggtg
     3301 gatgcggcag tcattccaaa cgttcgtata attgctgttc caataccgcc ttgatttctt
     3361 cgagaaattc cggaaatcgt gtccgcaact tgtctacctt gacgattggc tggtgaagca
     3421 agagaaataa gtgcatcttt tccagtgatt tgctgtttta caacgatagc atcccccacc
     3481 gcataaatat caggaatatt cgtttgataa cgttcgtcta ctaggatacc cccgcgtatg
     3541 cctaattcga ttccagcggt cactgctagc tggttatcag gacgaacacc aatggataaa
     3601 ataactaaat cagcagggat ggtacgccca tcatccagtc gcaatcgttt cccgtggtct
     3661 tcaaatccag cgacagcatg agaagtaatc acttctactc cttgatgaga caattctttt
     3721 tctataaaag ctgccatttc ttcatccagt gttggaagaa tatgtggtgc ttgttcgatc
     3781 accatgactt ctaaacctct tctttttagg ttttctgcca tttccaatcc gatgaatcct
     3841 gcaccaacga tcacggcacg ctttggctgt ttttctaatg catgcatcac tttatcgata
     3901 tctggtacat ttctaataga aaaaacattg tcggcttctg ttatccctgg aatcgatgga
     3961 acaactggtt ttgcaccagg agataaaacc aatgcatcgt actgttcaat gtgtttttga
     4021 ccctcatgta cgacttctac cgttttgttt tcgggaaaga tggctgtcac ttcatgattt
     4081 ggacgcacat ctaaacaaaa acgagcggat aatgattctg gcgtttggac aagaagattt
     4141 tcccgttcac tgatttctcc cgaaagataa taaggcaagc cgcaatttgc aaaagaaaca
     4201 tacggtcctt tttcaaaaac aacgatttct gcatcttcca ttaatcggcg aagccgtgta
     4261 gcagcagaca ttccacctgc tacacctccg acaataacga ttttcatctt atttccctcc
     4321 tactgttcga tcaaaccatt gattcatgcc accacgtaca ttcactacat cataaccaga
     4381 tttctttaat tctttagctg cttgtttact cctcatccct gattgacaaa tcacatagac
     4441 ggtcttttct ttgtctccat catatctgtt tatactttgt aatggaacat ttttagctcc
     4501 ttttatatgc ccgccccggt attctgcagg tgttctaaca tcaagtaacg ctggattttc
     4561 caaaagcttt tcctttaatt ctgggactgt tatcgatttt attgattgaa atagcaaatt
     4621 cattttctgc acctcattta catttttata aatacctcat ggggtatatt aaacaggagc
     4681 tttattaata tgtcaaccag aatgactctt catttttttc actacttttt atatttgcat
     4741 aaaataccga taaggggtat tatctaatta taaaaataga ggagaatgtg ttatggagat
     4801 tgataataaa aatatcctga accgtttgaa acgtacagag ggacaaattc gtggtatcca
     4861 aaaaatgatc gaagacgaaa aagaatgtat ggatgtcatc actcaattaa gcgctgttcg
     4921 ttctagtatt gatcgcgtaa tgggcatgat cgttgcagat aatctgaaaa attgctttga
     4981 gaaacctgat agtaatccag aagaacaagc ggctaaacta gaacaagcga tcaagatgat
     5041 tattaaaaag taaaggaatg atttcaatga aagtcgtttt ccatattgat gaattagaaa
     5101 aatggtcaga aacagggaaa aacgtaaaaa acctgataaa agcttctcca gaaacgacga
     5161 ttgtcgtatc tgtaaatggc atcgcgatta ccggttatct agactctgcc aatgcagaat
     5221 tcttagattt gcaaggagtc gtttttcacg cttgcgccaa tgctatgcgt gctaatcata
     5281 tttcagaaag ttctttacct gaacaagtaa tcgttgtacc agcaggtgtc cttgatttag
     5341 ttgaactgca atcccaaggt tatgcttata tcaagcctta gtcagtagag gttgcgacag
     5401 aagtgatcag cttcaagaaa tttcggctta tttttcgaag aagctacttc tggaaaaccg
     5461 tgaatcaaag gttgtgaaaa cgctgtcctg catcaagtaa taagaacagc caaacaaaaa
     5521 tagcttctca tatttttcgt ttggttgggc ttattaccgc agatgcaagc ttttgaacac
     5581 cgtttaagga agaggttgtg aaatcagcgg tttgacctaa gtattagaga aaaaaacggg
     5641 aaaatagctc ctcatatttt gccgtttttt gagctaatac cgaaggtcag ctgattgaac
     5701 accgtttaag gaagagattg tgaaaaagtc gcttagtttc gagtattaag aaaaattttg
     5761 tgaaaatgga tttctacatt tttgcataaa tttggcttaa taccgagaaa ctggcttttg
     5821 aataccgttt aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagaaacaa
     5881 acggaaaaat agctgctcat attttgccgt tttttgagct aataccgaag gtcagctgat
     5941 tgaacaccgt ttaccaagag gttgtgagcc taccatttaa ctccgaatca caagaaataa
     6001 tttcaaaaac aacttctcct gtttttggtt tggttggctt attatcgaga agtagctact
     6061 gggagtctat ttatcaaaaa aaggaatgcg acataggtca cacccctttt ttattttctt
     6121 tgaaacatct ttcggtactc gacagcatac aagacaaaag attgtaaaag accgtaagtg
     6181 atgacggcaa aaatgactct cacccacagc gttggtaatt ctgggaaaag cagcaaaagg
     6241 atcatgccaa catagatgac agcagtcgct actttgccat accacttcgc tccacttaat
     6301 ttgatatttt tagatagccc gattaatcca gctacaaaca taaagccttc ttttatcaag
     6361 aatagaccaa acaacagcca aagccacggt cgataccatg ccatagacaa aatcagaaaa
     6421 agttgtgtca atttatctgc aaacggatca agcaccttcc ctaagtctgt tacctgatta
     6481 tatctccgag caatgacccc gtctagataa tcagacaaac ctgagacaac aatgaccgct
     6541 cctgctatat aaaatgactg tctcagcaca aaataaagat aaatcggcag caaaaaaata
     6601 cggaatatcg ataaaagatt cggaatcgtt tgtatttctt tacgccagtc tgtgcttaat
     6661 ttcattttat cgccctacct cctcagcact cttttctttt tattttagct tacacgggga
     6721 ataaaaccaa tgatttcact gtttttattt ttctttacag ccactgatat tatgagctaa
     6781 tttgagataa tcacttgctg ttttcatcgt ttactcttct ttcttcctgt cgtttgcgca
     6841 tttcttttac tagtacttta tgagcttttt ttgatcctcg ctctaggtca cgctgccgtt
     6901 tacgtgtttt ataatcgata aacagactgt ctaaatcgta acctgtcggg tacagatctt
     6961 ctgctgaacg caccagacga acacgctgac gaggcacgtg gattattttt ttgtctaaat
     7021 atacatcaat cgtatcttca cctatatctt cataaactaa cgcttctttt tggtattcat
     7081 tgacaaatat ccgatcacct tttgcaaaga gctggcgttc ttttttattc tccttcctat
     7141 ttgtaactgc tgtcttagga agaaattctt ttttctgtgt ccgataggct ttttccgcca
     7201 agtaacgctc tgcttgatgg atcaatttca tcgacatatc cattttatga gcaatccaca
     7261 aagcttggct ttctcctgtt tctccaaccc gcaactgata tttaggacga agtgcttccc
     7321 gatcgaaagc catagcagcc ggcacaaaat cttcatgatc tccggcgaat ttcttgattt
     7381 ctccataatg ggtcgtggca atgatcaatg ccccttgctc atacatagat tccatgattg
     7441 cgatcgctag tgcagcccct tcatttggct ctgttccact gccaatctca tctaaaagta
     7501 ccaacgtatt cctttttatc tttttcaaaa tagcagcgat gttttgcata tgaccagaaa
     7561 atgtactcaa cgcattttct aaattttggt ggtcaccgac atcgacgaaa atctcatcaa
     7621 agacagcaag ctctgtccct tcttttgccg gaacttgcat accgaacatc gccatcaaag
     7681 tcaataaacc gactgtcttc aacacgactg ttttgcctcc ggcatttgct ccggtgataa
     7741 tcaagcctcg atagtcttta ccgatttcaa catccaatgg taccgcatgc tcaggtaaaa
     7801 atggatgtct gccttgcttg ataatgatat gttccgattt attgattcta ggtgtgcttc
     7861 cattgatttc tcggctaaat tttgcccgag caaagatgat atctaacacc gtgatcgttt
     7921 cgatcaataa atcgatcact tcttcgtttt ctgcgatagc accagtcaat gccgccagga
     7981 cttgatattc ttcactgatt tcttcagctt ttaacaactg atagtgttca ttcaatttag
     8041 aaatcacagt tggttcaata aaaaccgtcg ttcttttatt tgattcatcg ataatcgttc
     8101 catctatttt atttttatag ctggctttga taggaatcgt atagtattcg cctttttgaa
     8161 caatcattgc ttcttggatc atttctttgt ttttcggatg acgcaagaat ttcagtaatt
     8221 ttgactggat ttctttttcg attagttgta attgtttgcg tactttacgc aaattacgtg
     8281 acgcgtcgtc actgacttta ttatttttta ttttctggtc aatcaactct tctacattta
     8341 tcaaatcagg aagatgtttg ctataagcaa acaacaaggg tgtctggtat tgattcttat
     8401 caaaaaactt tgtgatcatc cgactgcttc taagaaaatc cgcatattcg atcaaatcgg
     8461 ttggctgcag aatcaaccct tttttgactt gttctgtcag tgcatcgatc ctcggcaacc
     8521 ccataaatgg aacatgctga ctgctttcta agatcaagcg tgcttctctt gtttcttctt
     8581 gacgatccaa gacagtttgt aggtttgtcg agaccgtcat atcgctgatt cgttttttgc
     8641 tatagtttcc aattgctctt gcttggattt cctttttgat ccgatcaaat tgtaattgac
     8701 taatcatttc gttgtccata ttctctcctt ttcttgaaga gatacctgtc ccaaaataaa
     8761 taagggccta gagataaata aaaggataga acacagctta aaaaagtcat gtcctatcct
     8821 tattagaata caaagatatt gttttcctga caggtaccgt ttcataagca caccaaataa
     8881 actttgttta ttggctattc gacttactga ttagatacca gctacactca caaaacatac
     8941 cccattcttc taggtcttat ttattttcca cttaactata gcaagacaca ttttggttgt
     9001 caaactttag tatgttttat agatttacac cattaatgaa tagacaccta ccgcgataat
     9061 tgctccgatc attggacctg cgattggtac ccatgaatat gcccaatctg aagttccttt
     9121 attagctatt ggcaataccg catgtgctaa tcgaggacca aagtcccgag cagggttgat
     9181 tgcatatcct gttggccctc ctaatgaaag accgatggct aagatcaatc cacctacagc
     9241 gaatacgttc gttccaggtg caaattcgtt ttgtccaaaa gctagcaacc caagaactaa
     9301 tacaaatgta ccaattaatt ctgtgatcac atttgctgga taattccgta tagcaggacc
     9361 tgttgcaaac gttcctaaaa tcgcgctttc atctttcgtt gcattccaat gtggcaaata
     9421 agaaagccaa acaagaatgg cccctgcaaa agctcctaaa acttgcgcaa tgatgaaagg
     9481 aacgacaaga ttccaactaa ttgcccctgt catcgccata gctagagaga ctgcaggatt
     9541 caaatgagct gggcccatga atccagaaat atagacagcc attgttacag cgagtcccca
     9601 accaaatgcg atgacaatcc agccagaagc ctgtgcttta cttttgttta aattcacagc
     9661 cgcacacaca ccatctccaa gcaaaactag gatcagtgtc ccaataaact ctcccatgat
     9721 ttgagtcata tcactgttca tttatttctc cccctttagt tcccgcagat ctgattctgc
     9781 aatcaatgct tctaaacgtt tagtctgttg cgccttctcc tcttctgtcc attcaaaata
     9841 tccagcaatt gcatcgatca caggttgctt gatctcatca agctgatctc gttcaaacaa
     9901 cagatgattt gtacgacgaa tgagataatc gcctggggcc aaaaccattt cttcttctaa
     9961 accataacgt aaccgagctg actcagcgag actcaaacca ggataaggtg ccatttcttt
    10021 tgctaattca aagatacgtc gggcattagt cccgtaaaaa tcagcgatat aagtagcatc
    10081 ttcttcctct aaaccggctg ctactccttc ttttgctaat tctgtcactg tttcttctaa
    10141 tttagttgga tcgaagtttc cacctgaaat ctgatatttt ttagagtcga tttctttcgt
    10201 ctctattccg tattcttctt ttaataattg gcgtatcaat cgcaaagctc cttctgccat
    10261 cttacggtaa tcggtgattt tcccaccaga taaagtcagc aaaccatctg gttctctttc
    10321 taaagaacta cctctggaaa tcgtagaagg tgctttttca tcacggctgt tttccaaatg
    10381 gttcaacacg tcttctactt cagcacgaga aactttgttt tccttatatt cacttactgt
    10441 atcgacaact ttattgaagc tcttgtctga aatcgatcca ttatctccac cattgtaatc
    10501 agaaccagaa ttaccaatca acagtggacg aagtcctgcc cagcttgctt cgatatcatt
    10561 caatgtgata tttgcttctg gatagcgatg attgatcaca tccaatagat aatctacatc
    10621 ttcttgtgtg actttcggat ccgtaaaatc tccttggtaa tccgtatctg tcgtaccaaa
    10681 gtaagtcttg ttttctcttg gaatagcaaa aaccatccgc ccatcttgct ttcctgtatc
    10741 gaagtatgtg ggttgcggta caggcagttt tttcgcatct acaactaaat ggatcccttt
    10801 agttggacgc attttaggag agactggacg cgtaaaattc aagtttcgta ctttatctac
    10861 ccaaggacca ctcgtattga tcactaattt tgctttgatt tcgatcactt catctgtcag
    10921 caggtcacga gctttgacgc caacgatttg atcgccatca tataaaaagc caaccgcttt
    10981 cattttactt actagataag ccccatcttc ggcagccttt ttgatattat caatcactaa
    11041 acgggcatca ttgttgcgga aatctagata gacacccgcg ccttttagcc cttctttttt
    11101 caaaaacggt tctctttcca atacttcttc tggtgtcagc gtataattct catatttggt
    11161 tcctgtcaca tttgccagtt tgtcataaag gtccattgct actttgaccg agaacatatt
    11221 gaaggttgtt gctccttcgc cctcatagat tggcaaaagc attggatctg gttttgggat
    11281 gtgtggggca atactttgta cgactgcacg ttcaccaact gtgtcagcta ctacttccac
    11341 atcaaatgtc ttcagataac gaataccgcc atgaacaagt ttggtcgagc gagaggaagt
    11401 cccttctgca aaatcctgca tttcgatcaa tcctgttttg attcctgatg ctgctgactg
    11461 gactgccaca ccggcaccag tgattccgcc accgatgatc aacagatcca attcttcttg
    11521 ctgcattttt tgaatgctat cttgtcttgt cttgtttgaa aacatatgga tttcctcctt
    11581 attcaaatgc ttgggtagct tttactgctt ttttccaacc gttgtaaagt ttttctcggc
    11641 gctcttcctc catttgaact tcaaatagct tgccagcttc ataaaattcg cggatctcgt
    11701 ctgtatcttt ccaataaccg acagctaatc ctgctaaaaa tgctgcccct agcgccgttg
    11761 tttccaagtt ttctgctcgt tggatcggta cattcaagat atctgtttgg aattgcatca
    11821 gatattcatt gtttgctgca ccgccatcta ctttcaatac aggaatcttg attccagtgt
    11881 catcttgcat cgtatcgatg atatctctca cttgataagc aatcgactgt aaagtagctt
    11941 taatgatatc ttcttttgtc gtaccacgtg tcaagccaaa catagcccct cgtgctgcct
    12001 gatcccaata aggagcacct agcccaacga atgcaggaac aacataaacc tcatcttcgc
    12061 ttgtagagcg tttcgctgcg tcttctgaat cgctagcttt ttgcaacatt tgaagtccat
    12121 ctctcagcca ttgaatcgaa gaaccagcca caaaaatact tccttctaaa gcatagtaaa
    12181 cctttccatt tatgccgtaa ccaattgtcg tcaataaatt atttttggat aattgcggtt
    12241 tttcaccggt attcatgaca ataaatgaac ctgtaccata agtattcttg accatgcctg
    12301 gttcgaatgc catttgtccg aataatgcgg cttgctggtc tcctgccatc cctgaaattg
    12361 gtgcttcgct tccataaaaa tgataacctt gcgtataacc ataaacttct gagttagaag
    12421 tgacttttgg taacatcact cgaggaatat ttagtaaatc taaaatctcc tgatcccaat
    12481 ctagctgatg gatattgaac aacatcgtcc gactggcatt cgagtaatca gtaacatgtg
    12541 cttgtccatc ggtcagtttc catactaacc aagaatcgat cgttccaaac atcaattcgc
    12601 ctttttcagc tcgttcttgc gcgccttcta catgatcaag aatccagcgt atcttcgttg
    12661 ctgagaaata ggcatcgatc actaaccctg ttttttcgtg gaaaaattct tgatagcctt
    12721 cattttttaa gttatctgca attccagctg actgtctgga ttgccaaaca actgcatgat
    12781 agattggacg tcctgtatct ttttcccata ttactgttgt ttcacgctgg ttcgttatgc
    12841 caatcccagc aatctgattt ggtttgatcc ctgattcgat gaacgctccg gcaataactg
    12901 actgtacaga attccagatt tcatttgcat catgttccac ccaactttct tgcgggaaat
    12961 attgcgtgaa ctctttttga gaactgccga tgtgtcttcc tttcttgtca aagataattg
    13021 ctcgtgagct agttgtacct tggtcaatcg ccatgatata cgttgattct gtcattgtga
    13081 ttccctccat tatatgcaaa cgctttcttt aactactctt atcatactac tgttttaaaa
    13141 aaagaaactg tcaaaatttt attctttttt tctttttttg acagtttttt attatgaagc
    13201 tattttttaa tcaattcttt tcttttttca gcgtctctat tcttcttttc aatcgaataa
    13261 tgtcgtaagg tgacgcaaat tctgacagat aatattcaca ctgttttttg tttctctggc
    13321 aaaatgtgat caccaaatca tattctttac cttcttcata acttccaatc tcaataccgc
    13381 cgattcccct cagttctctg atcagatatt gttgaaaagc aatacgataa atcggcagac
    13441 tttctaaatc gattccaatc actactggtt ttgcaatata aaaatcgatc atcaaaagaa
    13501 cattggcata ctggatcatc aggtaactaa gctcactatc ttcagctctt ttcctcggca
    13561 attgctgcac cgctgtttcc tgtaatttcg ccagtaactg tgttaaggaa tgcccgagca
    13621 ttttctgctg ctgttccaaa agccgatcac gctcatagac ttcgatcttt ccaacaaaga
    13681 aaaaccattc attgttgatc tgggaaattt ggtagcctac tgccttttct tctttgattt
    13741 tcaaacgacg aggacgataa tgccataaca tcgtttcccg aatgtaagta tctaaaacag
    13801 cggttggtaa ctttttgctc cgtgtcaaat catactgata aaatatttct tcgtctacta
    13861 tcgaaaaaga aacaaaaaag ctgtaaaaca tcattgtttc gtatacattc agttctgctg
    13921 ctgttcgact aagatgcatc acgatgattg gatcgattgc ttgataaagc cggtccgact
    13981 gatagagtaa tttcttttct ttcaaagttg cgtacgtact tttttcattc aatagtcttt
    14041 ttttcgtaat cccgagccag caagcaatct gtgcttctgc tgatgccgta aacgttgttt
    14101 ctaatacacg gtccaatcct agaatgaacg gtcttttttc tggtgtattt tgcaaaaaaa
    14161 gcggtcgctg atcttccggg atatattgga atagttcata ataaaaatat cgtatctgta
    14221 gctcttctcc gtataattga ccgtttttga tttgtaatcc aaattcctct aagagctggt
    14281 tcaattcttt gatctttcga aaaacagaag actcactgat attaaacgct gtcgctaatc
    14341 gaacgatgga tacttgctga tgttctaata aatagacgag catcttaaac ttcaagcttt
    14401 cctgcaataa aaacaataga attttttcca aacttacatc ttcgtccaac acaagcagcg
    14461 cctgatactc atttcgctgt acttctactt ttttttccat cattcggcca atcaattcaa
    14521 tatcttccaa atattgatca aacgaggctt ttgacagttg gacatgctct ctcaaaccat
    14581 tgatagaggc tgttccccct tgcagaacaa ggtttcgcaa aatcaggatc tgtaactgct
    14641 cttttctttc taataatgct tcaatcttca taggcagtat tcgccacctt tgaccacacc
    14701 atatctggca cacggtattc tggctggaaa aatagctcat gttcttctaa ctgttcaaac
    14761 gtccatcctt cttctactgc aattgtcagt tggtcaagaa ggtggaacat ctcttttttg
    14821 ctttggagct gtgctccaag aatctttttt tcagaatcaa atagaaattt gagtttaact
    14881 ttgctttgat ttattgctgt aagcttgtag tctacgtatc ctgtttcaat tttttgggga
    14941 taaaaaatgc tctcttcttc tgtcaaacct acgcttccta agaaatagcc aaaccactga
    15001 ttgccgactg gacgataggt tccctgatcc tttgtctgtg agccactaat tgtcttgctg
    15061 acatttcgtg ctgtacggac agcgttattt actagagaaa catacatttt ttcatttgtt
    15121 ggacgaaaag ttacttggat cgcatcgcca atcgcataaa cgtctttttg agacgtttgc
    15181 aagtagtcat ctacccagat cgttccatca tcattcaatg ttaattgttc ttcccacatc
    15241 tgattgtcgg ggcgagaatg attagccagc aaaacaagat catagttctc ctttcttttt
    15301 tccgtctcta caatagcggt ttcattttcg gtcaaagaca caacttgttc ttctaaaaaa
    15361 agtgaaagcc cttgattttt catttccttt actaacggtt ctgtcatttc aggatctaga
    15421 taacgaaaaa gaattgactt tcggctttcg taaagatgga tttgtttttt tcttgatgcc
    15481 aacccttcag ctatttcgat tcctgcttgt cctgcaccaa taattgcaat tttctttgca
    15541 ttcatgatct tttgctgaag agccgttacg ttttgtatct ttttaactgg gtgaataagc
    15601 gttgattctg cagaaatatt tcgaaacttt tgatttgaac cagtcgctag aatcaaacga
    15661 tcaaaatcca atttttttcc tcctgctaaa acgacttttt tttctttcaa tcctattact
    15721 gtcttttctg tgtaaacatg gattcgatag cggtcagtca gttcttcttt cgtgatccaa
    15781 gaaagttgtt tttcactggt aacctttcct tttaatatca aattgaaccc gctgggaatc
    15841 catcctaact tcgattgctt ttcaatcaaa tatatttcac ttgttggcat caattggcga
    15901 agataaatag ccgcatgaat ccctgcaaac gacccgccga caattacaat gttcatctgc
    15961 attcctcttt cttataaggt gttctactgc taacttctca atcagaaaac acagtattca
    16021 taactcgctt tcaaccttat gatacatagc gatgcagcag aatcaaccct ctccatcata
    16081 ccgtgttcta ggtctgcttc tcaataatgt actagtggac ctaattgaag agtatcattt
    16141 ctgaagagat gtttcaaaaa atgtactctt ataaaagaaa aaaactcgcc ttttacgtac
    16201 ctctgtccaa ataggaaaca gaaaagaacg aaaagacgag ttttagcgtg tgtaatgtaa
    16261 tagttagaat agacacatca tttattttta attctttaat gcttcaacaa ctgcttcgcc
    16321 taattgatga gcagattgag gattttgccc tgtaagaagc cgcccatcaa tttgaaccac
    16381 ttctgaaaaa gctgctccag actgatagtt tgctcctttt tctttcaaag catcttctgc
    16441 taagaatggt acttcattcg ttgtaccatt tgcttcctct tcttcattac tgaacccagt
    16501 cactgtacgt ccagctaaaa tagattttcc agactcatct gtaactggca acaatcctac
    16561 aacaccgtga caaacagctg agaccagccc ttcatttcga tagatttgtg aagcgacttt
    16621 cccaagatct attgcttctg gaaaatccca cattgttccg tggccaccag cataataaac
    16681 cgctgcataa tcatttgcat ccacatcatc aggacgtaag gtatttccca aggcccgatt
    16741 tctgaaatct tcatcttcat aataggccca atcgatttca tccatattca aaacactttg
    16801 cggttctaaa ggtacatatc cgcctttagg actgacaaaa tctgcatcga tcccattttt
    16861 tactaataca tcataaaaat gagtcagttc acttaaccac aaacctgtag gacgctcgat
    16921 cttttcgtac ttttctacat ttgttaatac gatcaatact ttcattttcc catcctcttt
    16981 ctataaaaat tgcgttattg ataacgtgct ttcaagaaat tatataatgg gcttggcaaa
    17041 taaagctctt tatatgcttc ttcaaaaggt ttcgttacaa attcatcctc acggttttcc
    17101 aataatttct ctgcccagtc aggatcgacg atcatttgct gtccaattgc tgcaagttct
    17161 gcatatttca atacctgctc tacatcttcg cgattgcgga tcccaccgac accaactaaa
    17221 gggatctttc cttgaatcgt gttatggacc acttcaagaa tcgatttatc gttgtattta
    17281 tcagaacgag ctattcgatc atacatattt aatgaaacat gcagataatc tagttttgac
    17341 tcacgtaatc gttcaagaag ccagatcgtg tcttcaaaac ggatacctgg tgtttcatat
    17401 tcttctggtg agaaacgata ccctacgatg aatggttttt tagcatattg ttcaaccgct
    17461 gagaaaaccg ttgttaataa ttcttcaata aaacgataac gattttccaa gctaccgccc
    17521 cattgatcct ctcgtctatt cgaatgcgga gagaaaaatt gttggatcaa atacgtattt
    17581 gctccatgca actcgattcc atcaaatcct gattcaatcg ctctacgtgt ggctttgcca
    17641 aatgcttgga ttgtttcaac gatttcttct tcactcattg ctcgtggtgt ttccgcatca
    17701 ggacgttttg ctgcaactgc acttgcagat acaggctgtt ctccagataa agttgctcga
    17761 cttgtcattc gtccaccgtg gaaaatctga ataatcgctt ttgcgccttc tccttggatt
    17821 gctttcgcta gctcacttaa tcgaggaatc atttcatcat gagcgatgct gagttcacct
    17881 ggccagcctt ttccgatatc ttggacattt gcagcaccag taataacagc accaagacct
    17941 ttagaacgtt gtttataata agtgatctca tcttcggtga tcactccatt gaaaaagctg
    18001 agttgtgtcg tcataggcga cagcatcaat cggttcttca attgaactcc tgaaggtaaa
    18061 gtaattgatt gatcaaattg tgacattgtc attcctccca taattcgctc tgattaacct
    18121 atcgagaatc atctatcatt aaaccactcg attagttgta catgcaacta atttgcttaa
    18181 aaaattttat tttttttgta acttatctgt tgcatcattc attgtctggg ctaattcttt
    18241 acttgcttca tttcccaaaa ctgctttgta tgactgatga agttcttccc agctaccatc
    18301 gacttcctta gcaaattggc gtccttgttc tgtaagataa actaatgaca agcgaccttc
    18361 gctaactgtt ttgacgtatc ctttatattc taatttttca acaaaacgag caatcgttga
    18421 tggggcggaa tagagcattt gtgcaatatc tttttgtggt attccatctt tttcttttaa
    18481 tatcaataaa ataaaaccat aagttggtgc cagacctgtc cttctaaacg cttcatcagc
    18541 taatttattc aacatgcgat ctagcttttt cacgctaaag aaaagacatt catctaacgc
    18601 tttttcaatt ggcttttcca tttcatcccc tccattggcg tacatatcat agtatgattt
    18661 ttattttttg tcaaaaaaca cggaataatg tccgcctcct ctgtctagat ttgaaaaaaa
    18721 gaagtagcca tcttcaaaga atttcggctt gttttcgaca aaactccttc tggaacaccg
    18781 tgaatcagag gttgtgaaaa agtcgtttag ttttgagtat gaagaaaaat tttgtgaaaa
    18841 tggctttcca catttttgca taaatttggc ttaataccga aaaactggct tttgaacacc
    18901 gtttatatca gaggttgtga aaaagccgtc ctgcatcaag taataagaac agccaaacaa
    18961 aaatagttcc tcatattttg tcgttttttg agctaatgcc gatggtcagc tgattgtaca
    19021 ccgtttattg agaggtcgtg agcctgccgt tgaactccga gtcacaagga gcaatttcaa
    19081 aaaacagctt ctcatgtttt atgactatgg tgacttgtga tcgaggagtt ggcaggagaa
    19141 tactgtttat tcgtaaagaa ggggctgtga caagactttt gtcacagtcc cttatttcct
    19201 tttattatag gatttcgcca tttgattgga tgacttgtct gtaccaatca aatgattttt
    19261 tctttaaccg tttctttgtg ccattccctt cgtcatcttg atcaacatag atatatccat
    19321 aacgttttga catttgtgaa gtagaagcgc tgacgatatc aatgcagccc cacgacgtat
    19381 aaccaaataa atctactcca tctttgatag cttccttcat ctgctcaata tgctgtttca
    19441 aatagtcaat gcggtaatcg tcttgaacgg ttccatcttc ttctactttg tctatagctc
    19501 cgagtccgtt ttcagctatg aacagtggtt tttgatacct atcatataat tgattcaaag
    19561 caattctcaa acccttagga tcaatctgcc agccccattc acttgatgga agatacggat
    19621 ttttaattcc tccagttatt aagtttcctt ggaccccttc ccaattttct ggatgagcac
    19681 tgactgcact agacatgtag tagctaaaag aaacaaaatc aacagggtag gctgctaaga
    19741 tttcttcatc acctacttct atttttaagt tgacaccttt ttcttcaaac attcgttgcg
    19801 tatacgctgg ataatatcct cttgcttgaa catcactaaa gaacaaagaa gttctttgct
    19861 gctcaattgt tttttgaata tcatccggat cacaagaata cggaacaaga agctgatagc
    19921 tgatcataca accgacttgt gaacctttga taatttcatg acaagctttt gtagctaaag
    19981 cacttgctat aaactgatgg tgtgcacctt ggtatttcgc ttgttccaaa tggggatggt
    20041 tttcttcaat tactcctacg ctaacaaaag ggtgatgttt tacacagttg atctcattga
    20101 aagtcatcca atattttact ttatttttat agcgtttgaa tactgtttca gcgaaacgaa
    20161 cgtaaaactg aataagcttt cgattaggcc atccaccata ttctgtaacc aaatataacg
    20221 gcatttcata atgggaaaga gaaacgactg gctcaatccc atattttaga cattcgtcaa
    20281 acacttgatc ataaaaaaat aatcccgctt cattgggtgt ttcttcatca ccatgaggaa
    20341 aaattcttgt ccatgcgata gacatccgat agcatttaaa acccatttca gcaaataaac
    20401 ggatgtcttc tttaaagtga tgatagaaat catttccgtg tcttttagga taataatact
    20461 tgctgttggg atcctgcgcc gcttttactt gctgatgcgt catcatatgc cattgatctg
    20521 tccatttttc tttaggtaaa tcgctgtcga atgtatacac atcagacaca gataatcctt
    20581 tttcatctac atcccatgca ccttcacatt gattggccgc tgtcgctccg ccccataaaa
    20641 atccttctgg aaattttttc atatcgatcc ccctaaactt gctagttttc ttgtttgatt
    20701 atttttctta tgactagtaa tgatattttt gaatctacta ctatttgtaa caataaccat
    20761 tgtggtcgga tcatatccct ctttgattaa ttcttgcaaa tctacttcca tcaataaatc
    20821 gcctttacgt actttttttc catcagatac tgcactttga aagtatttgc catttaaatt
    20881 cactgtatca ataccgatat ggatcagtat ctctgctcct tcttcactga ctaccccaat
    20941 tgcatgtttc gttggaaaaa ctgttgaaac ttccccgttt acaggactgt agatttttcc
    21001 gtccgttgga atgatcgcaa acccttctcc taaagatcca ctagcaaatg ttggatcgtt
    21061 tacttcagat aaatcaatta tttcacctgc tgcaggagta cttaacacta tatcctttga
    21121 ggattcttct tcgatttcat ccttgtagcc gaaaagaaaa gttagcatac aggaactgat
    21181 catcgctaag aaataactag caactagcca aacaaatcct tcaccaatat aaatcgggaa
    21241 agacaaaata cttcttgtag caactgccaa agcaccggct cctgcggcac cagctactcc
    21301 accaaatata gctccacata tacaagcaat gtaaaatggt cgtttatatt taagagtcac
    21361 accataaaca gcaggttcag tgattccaaa taatgctgtg ataaatgcag ataaagataa
    21421 actttttact ttcttatttt tcgttttcaa aaatacccca agtactgctc ctgcttgcgc
    21481 agctacggca gtcatacatg ctggacctag cgtatcacga ccgtagaaag aaagattgtt
    21541 ggtcattact ggtacaagtc cccaatgtaa cccaaaaatc actaacactt gccaaaatcc
    21601 gccgataaca gcaccagata aaacagggct aaagttataa acagctaaat atccagctgc
    21661 aatcagctga cttgcataat ctgcaattgg acctacaact aataaagtaa ttgggaccat
    21721 cacgatgata caaatgaatg gtgtcaataa atttcgaata ctttcgtgca agcttttgcg
    21781 aatcttaggc tcaatataag aaagaaccca tacagctaaa attgctggaa taaccgtact
    21841 tgtatacctt gctagaatca cgggcatccc tagaaatcgc aaggttatgc tatcactata
    21901 tgcactgaca atcgtaggat aaacgagagc tgctgcaata accatagcga tgaaacgatc
    21961 tgtattgaac ttttttgcag cagtatatgc caaaaatata ggcaagaaat agaaaaatgc
    22021 atcagctgct gcattcaaaa tgatataagt tcctgattca gtagttaata aattcaaact
    22081 cgtacataag gcgagtactc ctttgatcaa accacttcct gccaagacgc ctaaagtcgg
    22141 ggcaaaaaca gcggcgatca tgtctataaa agagtttaaa atgccttttt tctcagttcc
    22201 tctctttcca ctagatgcat catcttctag tttcatttgt ttggataata cgtcatacac
    22261 ttcatttact gcatttccta ctacgacttg aaacattcct ccagcttcga ctacagtgat
    22321 cacaccttct agttgctcta ccttctcctt atctgccttc ttattatctt ttagggtaaa
    22381 gcgtaacctt gttgcacaat ggtaaacatt gctgacattt tcttcgcctc cgacaaatcg
    22441 gacaatatct ttcgctaatt gtgaatagtc catttttact tcccccttgt taattaatgc
    22501 cgtagcaatc cttcttacaa gataagtata gttcttagaa aacggtttct ccattcaatt
    22561 agtacccctt ataatagcga acgaaactgt acaatagtgt gtatggttta cttagccgag
    22621 caaatcactc actgcttgaa agaaaacaag tatatcgttt ctcttatagt caaaggagac
    22681 aaaaaaaaca cgcttgatct tcctttgctg atgtataagt ggtatctcaa gctgatactc
    22741 tgtgattttt gtttctactt ggcttttgat aagacagcac aacaaagtct cggcatttaa
    22801 cggaagcata actaggtcag aactaggaga gattagttgc tgaatggaat ctatcttttt
    22861 atggattttt atttgatttt cttctttgta gtaatgatca agcagttttg ttttcttttt
    22921 ttcttcaact atggtcagct tttcacgcaa aatctgataa atctcccaag attcaaactc
    22981 atctagaaca ctaggtagat aaatcaaatc atcccactca aaaataagct cttgctcagt
    23041 agtcaaaatt gcatcatatt ccgttatttt gtttgattga tttttgaaaa gatacactgg
    23101 ttctatccgg cattctttga tccacatgtt catactttgt tgaatctgtt tttttagtgt
    23161 attcaaaata ctaattggct ggttactaac aatcagtatc tccccttgtt ttcgaaaagg
    23221 attcaacgct tcggcaagat acaaaacaaa ataagcgctt tcagctaaat tgggacgtaa
    23281 ttgaaaatgt attgggaaga aacgccgaat taaataaatt tctaaaggat aacgggagat
    23341 gatcttttgt acaaaatgat tcatggtatt atgtccggca tccatcctcg ttttcatttg
    23401 tttgatatgg gttagcaaaa attctgtttc atggaacaat aacgaaacag gaagatctaa
    23461 aaaacgaata atcgtttgct caaatgctct tagattcttc tctatttctt tgtttttttc
    23521 atcattaatc atcaaataat ttaactctaa tagtcgattt tcaattgctt gtttttcttg
    23581 attggaaaaa gaatagggta gttctgatga taactttttt gtgagggtct ctaccatcgg
    23641 aataaataat gtatacctgt ttgttttttc taaaagttct tctcttcgaa gaatggtcaa
    23701 aacgagatac cgagaaaatc gtatgaagtc ctctcctgaa acaatataat tatatgatat
    23761 aagaacctct tttaatgtct tttggacagt cggcatgata ttggaaaaaa ttgtactgat
    23821 ttgttgaggc aaccttgcgt gttggatcac cgtttctgtt ccaacaagag ataagaaaat
    23881 tctttttgaa ttttcttcag caatgatttt tagtcctttt gtcgacgaaa cttccagttc
    23941 taatttagga ttgcgctcga tccaacgcaa aatcgttttt atttgtaatg atacggctgt
    24001 ttttgaaaga aagaaaatgt ctgctatctt ttgcatactg acataccctt tttgaaacaa
    24061 taagtacgtc acaatagttg aagctcggaa atccatgctg gaatagattt tatgtttcgt
    24121 cacctcttgt atgatttgct gtttagtttc agattcaatt gattctaaaa aataacctga
    24181 acgtttattt gactggataa tgattttctt ctcgtagata gcaagcatat ttttgatttc
    24241 agtttggatc gttttcttgt tggtacctaa agcttcagct aattcttcgc ttgttatcca
    24301 atgattctta tttcttaata ctacaagaat ttggagttgt cgtttattta agtccatttg
    24361 cttacacctc ctctttgtta taaacataca ggaaaaacct ctcaaatgac aatagtcact
    24421 tgcagaggtt taaaaatcat ttgaagaaag atctatattc aaggttttta ccattagact
    24481 ggataatttg tttataatat tcaaaactat cttttttcaa tctcctaaag cttccgctcc
    24541 catcattttg tctatccaca taaataaagc catatcgctt cctcatttcc cctgttcctg
    24601 ccgatacgat atcgattggt ccccaccata agtaaccaat gatttggcaa ccatctttta
    24661 ttgcctcagc aatttcctga agatgtattt ttagatactc acaacgtttt ggatcgtgga
    24721 ttcgattcaa ttcatctggt gcttcatcta gtccaatgcc attttcaact atgaataatg
    24781 gtaattcata acggtctgtc aattcattca tgacataacg aaaccctaga ggatcaattg
    24841 gccaactcca aggttcggga gatttttcct tcaaaaaggg attatcgaaa ccatttgctc
    24901 cgccagtatc tacgcgcatt tctgcttctt ttttatagac agcgctacga taataactaa
    24961 atgcaatata atctgccgtg ttagcttgca agcactgttc gtctccatct aacattactg
    25021 gtgcacagtc ttcttccttc catgtcctgt aaatataagc tggataatgc cctcggcaca
    25081 tgacatcgga gaaaaaccat gaattgcgtt taaattgcag tgaaccaaat acattctctg
    25141 gatcacagtc atatgcataa gtcgctaatg cagaaaagct acacataata cccatcttag
    25201 catttggatc gattttcttt aataattgag tagcacgggc gttcgctaga aacatatgat
    25261 gacaggcttg ataaatcact ttttttgtta gtgtagcaat gggttcaagt ttatctctag
    25321 cagggaatgt aaccaatcca gcagctgcat aaggcatacg aaaagcatta ttgatctcat
    25381 taaaggtcat ccaatatttc actttccctt ggtagcgttc aaagacggtc tgcgcataaa
    25441 attcaaaaaa gtcgattaat tttcgatttt gccacccccc atactctcct accaatgcta
    25501 agggggtttc ataatgacta agagtaatta ctggctcgat atcatatcga tttaatgtat
    25561 tgattagtct gtcataaaat acgagaccag cctcatttgg cttctgttcg tctcctcttg
    25621 gaaatattcg tgcccatgag atgcttgttc gaaaagcttt taaacctaac tccttcagta
    25681 aacgaatatc ctcttcaaat cgatgataaa aatcaatcgc ttcatgagaa agataatgga
    25741 gtgattcgtc taaagcaatc tcccaaatct ttttttcttc attccactga atactaggat
    25801 gctggttcat gatcccgata agcgtatctg tcacattagg taatttgccg tcttccaacc
    25861 atgcaccttc acattgatta gcagctactg ctccgcccca taaaaaattt tctggaaata
    25921 tttcctgatc cataactatc tctcctcgct atcaacaatt tcttacttga agtatagctc
    25981 gattcatcaa aacaagatat tctaactatt tcggcagaag agaagaaaaa aattgaatgc
    26041 tactcttcat ttttaatatt tatatataac aaatacataa gacgatcccc gaaagaaaac
    26101 actcgttaac catctaagca aacgatttat tttacttcca acacaaatgc ttcataaggt
    26161 cgtaaatcaa ctttgccgtc tggtaactgt ttcagaggat aatttgacac aataaccctt
    26221 aaagcttcac gtggaacagt gaacgtctgc ttttcactgg acatattaca aataacgagc
    26281 cattgctggt cttcatatat gcgagaatag gcaaagacat gctcaggcgt cttttccact
    26341 aattcatagt ctccccagac gatgatgtca tgttttctac gcaaatcgat caatttcttg
    26401 tacatataaa aaacagaatc ttgatctgct aacgcttctt ctgcattgat ttctttataa
    26461 tttggattga ctcgaagcca tggaattcca gtagtaaacc cagcatgttc acgggcgttc
    26521 cattgcatag gcgtcctagc attgtctctt cctttcgcat tgattgattc caagatttcc
    26581 tcttttgtaa atccagaaga aatcctttcg ttatacatat tgattgtctc aatatctttt
    26641 gcttccgcga tatcatcgat tgggcaattc gtcatgccta actcttctcc ttgatagata
    26701 taaggggtgc ctttcatcat atggagcaag atagcaaaca tcttggcact ttcttttcga
    26761 tacacatcgt catttcccca acgagaaaca attcgcggaa gatcgtgatt attccaaaat
    26821 aaactattcc atccttcatc acctaaagat gtttgccatt tggataatac ttttttcagt
    26881 tcagagatac tcaagggttt caaatcccat ttgtctttgc cttcttgctg atctaatcct
    26941 atgtgttcga attggaagac catggataat tcatttcttt taggattgga ataaagttta
    27001 gcgatttcgg tagttgctcc ccatgtttcg ccaacagtca tcagattttt gtcaccaaaa
    27061 gttgcttgat tcatttcctg aagatactca tgcagtttag gtccattact tgtgatttct
    27121 ttatccggtt ctttgccaat catatcgatc acgtccatac gaaagccccc aataccttta
    27181 tcgatccaga aattcatcat ttgataaatc tcttggcgaa cctgtttatt tttccaattc
    27241 aaatcaggtt gacgcttgct atacaagtgc agaaaatatt gccccgtttt ttcatccaac
    27301 tgccaagcag aaccactgaa tattgagcgc aatctattag gctcttttcc attgaccgga
    27361 tcacgccaaa tataatagtc ccgataagga ttttctttat ttttacgggc ctcaatgaac
    27421 cacggatgtt catcagaagt atgattcacc accagatcca tgatgatacg gatatttctt
    27481 ttctttgctt cttcaatcag ttcttccatt tcttccatcg taccgaattc tgccatgatt
    27541 gcttgataat tactgatatc atacccattg tcatcattcg gagactcgta tactggagaa
    27601 agccaaattg catcaatacc taaatacgcc agataatcca acctggaaat aatcccccgc
    27661 aaatctccaa taccatcttg atttgaatct tgaaaacttc ttggatagat ttgataaaca
    27721 actgaatggt gccaccattt ttgttccatg attttcctcc tataaatgga tgtttctatt
    27781 ttttgcttaa tcatttattt tagtctctct cactaaaaat tgtaacgctt tcaaatagct
    27841 attgcacctt gttatcggta acagaataaa acaaaaaaac agctgaggaa attccctcaa
    27901 ctgttttttg aaaggattga tttatgcagg gaagaatgct gatttcacaa cttctttata
    27961 aacggtgttc actcagctgt cccttggtat tagctcaaaa aacggcaaaa tatgaggaac
    28021 tattttttcg tttttttctc taatactcag gccagaacac tgatttcaca acctctttat
    28081 aaacggtgtt cactcagctg tccctcggta ttagctcaaa aaacggcaaa atataagaaa
    28141 ctattttttc gtttttttct ctaatactca ggccagaacg ctgatttcac aacctcttta
    28201 tttcacattc aatatccaca tgaatcccac aaatacaaag aacagaccat acatgattgg
    28261 agatacttct tttcctcttt tagctgctaa catggtaatt ggatagaaga taaaacctaa
    28321 tgcaatccca tctgaaatac tataagtcaa aggcatgccg atcaagatca agaatgaagg
    28381 gatcgcaatc tctaagtttc cccattcgat ttgtcgcaaa gattgtgcca tcaatactcc
    28441 taccataatc aatgctggtg cagttacttg tgaagtaaca actgatagta atggagaaaa
    28501 gaacaagcct aacaagaaga aaatccctgt agtaatagct gtgatacctg aacgtcctcc
    28561 aacggcgata ccagctgatg attctacaaa tgcaccaaca ggagaagtac caaataagga
    28621 acccgcaagc atagctgttg aatcagaagc taaagctttc cctacacgag gcatcttgtt
    28681 gtctttcatg aaacctgctt ggtttgctaa tccaactaaa gttcctgctg tatcaaagaa
    28741 agtcacaagc aagaatgtca gcactacgac ccacatttga agagaattga tgtcccctac
    28801 atgttttaat gcaactaaaa atgttggttt caaacttggt gctgcggcaa caatttcaga
    28861 aggcattgga atcaaacctg tgaccagacc taaaatcgtt gtagctgcta tcccgatgaa
    28921 gatgccacct ggtacacgcc gaacaagcaa gatagcagtt acgactaatc caaaaatcgt
    28981 caaccaagta gaaccaacgt tgagtgatcc taatcctact aatgtagatt cattcgaaac
    29041 gatgatccca ccttcgctca gaccaaggaa agcgataaat agaccgatac cgccagaaat
    29101 cgcatatttc aaatctgctg ggatcgcatc aatgatcatt tcccgcagct tgaagattgt
    29161 gatcaaaata aaaatcaaag aagcaacgaa tacgccggct aatgctgttt cccatggaat
    29221 ccccattcct aaacaaacag aataagcaaa aaaagcattg atcccaagag caggtgccgt
    29281 tccaattgga taacgggcta atacccccat taaaacacag ccgatcgcac tggctaatgc
    29341 agtcgcagta aacactgctc cttcatccat tccagaagca cctaatacag ttggatttac
    29401 aaataagata taagccattg agataaatgt tgtaaatcct gccagtacct cacgttttac
    29461 cgtggtgttc aatttttcta tttcgaagta ggaaagtatt ttttccttca tcttccatcc
    29521 ccctaaatcc gaacattata aataaagtcc gttaaaattt cgatttatta ataaaacaac
    29581 gtgttttatc atatactaaa acaaaacata agtaaacaaa aaattcggat tttaagcaaa
    29641 atccgaacgt tccatctgat taaatccctt tatctcaaag ttcatgaaca ttcactttgg
    29701 attacaagaa tttctttatt gtgttaataa agtttcttca ttctagtctc aaaaatcaat
    29761 agtgtgattt gattttacca tttcgtttct gttcttcttt gatgaattct tttgtatcga
    29821 caggattcag attatttagt ccgccaaaac ctgtatcgac catttttccc cagccttttg
    29881 tcttcttttt tcctgatttg agattctttg ctcgaagctt ctcactatgt tttgctcgcc
    29941 gatacttcaa ataaccttct aatcttccca ttagatccac tgtttttcct cctttttatt
    30001 caatcagacg actcagcttt tcctataaca aaataattgc cttcagggtc agaaaaattg
    30061 aaagtcaaga tgccattgtt atctgtgatt tctcctggtt cttccatgtt ctcgtagata
    30121 gtttcaaatt catccgtaaa aaatactaaa gaaggcggtg gtcctaatac ctctggtgaa
    30181 aacttctgca caaatacttt tgggaatacg cctaattcta attcttttga gatgctgagt
    30241 acaatactct taaattcttc tggcaattcg attgtttcag aaatttctgc accaattttt
    30301 tctacaaaga atcggcaaat catctctacg tcattgacat aaagcatcac acgtgttttt
    30361 tgtatcatca tttgaccctt ctttctattt gattttcttt cctgtctata aactgattcc
    30421 attatatcac gaaagcgcaa acaccttgtg tttgatacta ttcaagaaaa ataaagcaat
    30481 agtttctagg attgctttcc tccacaaact ttttttacaa aaaaaaaaaa cggcaatgaa
    30541 tgccgctctt cttatttcga ttcttttata attctgcttc tgattcatct ttggttagtg
    30601 tgtgtctaac actagttacg ccaaatgttt gatcgatttt ttccttcaac gcttcgattg
    30661 cctgtgcgcc acgtgccgct tgttcagcat tgatggattc aatgaccagc accgctgctt
    30721 gtgtttcttc cgtcagcatt gttctttggg cttcacgcca gttgagacag cgacagatcg
    30781 ctccttgttc atcatagtag atgatttctt ccggaagtgc cggagcatcg gaatctgcac
    30841 ctaaaggcag gaatgattcg ccaccttggg cttttcctaa atgcagattt cctgcgatct
    30901 tttcgatatc ttctccgccg caaggtacgc caaaagacaa ggaaatacta ttatagatat
    30961 ctacaagcgg attgattggg aaaaattccc gttcttgatt tgcacgtttc agtaaagctt
    31021 caatagaaga acgtgcacct tttttagtct tgaatgtggt aaatgcctga cgccactgag
    31081 caatgacttc gttttggctg aatgtttctt ctgtaaggta atttcttgct tcttttgctg
    31141 cctcattcaa taattgatga tacgtttctt cactttcttt tgttacgtgg ttatcgatac
    31201 catttacgga taagatttcg atggttgctt caggaaatac ttcccagaaa ttggtatcca
    31261 ctattacttt tttcatctgt tcacacctct tgattagaat aattgcttga tgaatagata
    31321 gtctgtttgg ggatcatctc ccatataaaa aacatgttcg ctgtatttga taaatccttt
    31381 tttacgataa aaagcgagcg caacatcgtt gtgttcccaa acaccgagcc aaatggctgt
    31441 tttttgcttt tcttttgctt tttcaatagc tgtatctagt aaaaatgtac cgataccttg
    31501 atgtttaaat ttttttcgta catagattcg ctcgatttct aaaaaatgat cagccatttc
    31561 ctcagattga ctcgttcctg tatttagttt caggtaaccc gcaggctctt gatcgatact
    31621 cactaggaaa aaatcggttt ctttatcttt tagctcttct gtaagttttt ctagtgcata
    31681 agcttgtgtc aagtaatcag ctaaatgctc cggtgaatta tcttttccaa atgtatctgt
    31741 aaatgtttcg atactaattt gttgtagtaa gtctacatct gaaggcgtca tttttcgaat
    31801 ttcgtattta ttcacggctt cttcctttca atagatcctt ttattgcctt ttttgacaaa
    31861 attccaatct tcgctaacat tcttgcatac tttatttaaa agatattcca attgttttgc
    31921 ttcttcgtca gaaagaccat ttaatgcgac tgcattggag tagttgtttt ctcgaatgat
    31981 gaaaggatag atttctgctc cttttttagt cggatagagt ttcttgattt ttttattttc
    32041 tttatcttcc aaacgttcga tcatcccatt ggattctaat ttcttgattg ctcgggcagt
    32101 cgttgtacga tctactttga tcatttctgc tagtttttct tggatgattc caggattttc
    32161 gcaaatacgt actaaataaa gatactgtcc tcttgttaaa tcgacttctt tgaattcgat
    32221 attgctaata gaatctagcg ctcgggcaat cattccgatg tctcgaagga tttcagccat
    32281 ttcatcccac ctttattttg tttgtaagac tagtttacct ctttttgttg caaatgcaac
    32341 tattttcaat aaaaaaatca agttcttttt taataactag taaagtatat tcttttcttc
    32401 ttttacgaaa aagctcaatc acaatagatc tggtttttgt tttctaaaat aagtaactgt
    32461 ttcttcgttg ttcgcttttc tgatagcttt ggcaactctt ctatactaaa aaatcccatg
    32521 ttggaagttt cattattctc aataaacttg gcctcttcac cgtggatcgc acaagcaaaa
    32581 acaagtttat aatactgaaa aagctgtgga atatcttttt gtttattcgt atcaaaaact
    32641 gctcgcaact gagtagtttc tactgttaat cctgtttctt catagacttc tttacgcaca
    32701 ttttcttcag gagacaatcc tatttcagca aatccaccag gaagtgacca ttctttcgtc
    32761 cgtttatctt cgattagtag gaccttgcca tcttttttga tataggcacg aacatctact
    32821 tttggtgttg gatgtccttc tccaatcgtt gtgagcgctt ttatttcttc gaaagtttct
    32881 tttcctattt cactgatcag ttccaatgaa atcgtccgga tctcctcaaa acgctcttta
    32941 tcaaatacat cttttccata atatagacca gcttgagaaa gtgcgagtag tcttttgtat
    33001 tgagccaact tcttgttcat tctcttccct tcttttttag caaatgtatg actatgtaat
    33061 caagtataga agactggttt aattacggca aataaatata atgaaatctg caataaataa
    33121 aagaatgtta aaaaactcga gaacaaaaga cattcgactt ctctggggct gtgaataaat
    33181 ggcgttcaaa agcttgcatc tgcggtaata agccccacca aacgaaaaat atgacgagct
    33241 attcttgtct ggctgttctt attaattgag gcaggacagc tttttcacaa cctccccact
    33301 aaacggtgtt ctcctaccaa ctcctcggtc acaagtcaca atattcatca aacatgagaa
    33361 gctgtttttt tgaaattgct ccttgtgact cggagctgaa cgacaggctc acgacctctc
    33421 ggtaaacagc gttcaaaagc caatttttcg atattaagcc atttctttgt tacttattgt
    33481 cattgatttc tttttctaat ttatccaata gtggagtgta atccacttga gtatagtctg
    33541 ttacatcaaa ttcatatagt gttcctaact ggaatttgtc atattttcgg tcggcgatga
    33601 tctttttgaa tgcttctttc gtaatatgat tggtcccttt cgttcgatct tccaatacgt
    33661 caccatagtg ataatgatcc atgatatagc tcaaatgacg atctgattca cgatcacgtt
    33721 tgtaccgacg ttgacataac acttcaaagt ctgctgttag gcgaatcgtg atcacctgat
    33781 atccgtatct atctgctaat tcttggagtc gtgctttttg cttatcacta aaaggatact
    33841 cggacacaat cactttcttt cctgcttcca tatacaaatc aagtgctttg taatagaaag
    33901 gataaacttt cgtcctttct tgttccgccc gttcttccaa attgttaaac ccgatggatt
    33961 ctgcatagta ttccttgatt tcatcgagcg tgatggcata catatctgga aatcgttccc
    34021 gtagtttatt cataagatat gtttttcctg taccaggact accagccaga agaatgaaat
    34081 actttttcat ctcatttatt tcctaagtta attggacttt ccaagttcat aaacagtcca
    34141 atcttatgct tgacaatttc ttttactgct tcatttgctg gaatcacatt atggcgaaga
    34201 gacttgttga tttctgtttc actaaaggtt tctttagcta ctttcgtcca gttgactaaa
    34261 agctccgttc caacattgaa tttgcggatc ccgttattga tcaattcagg ataatcttct
    34321 tctttcacac ctgttcctcc atggataact agggggacat caactactgc atcgatttct
    34381 ttcagcaagg gaatgtttac ttctgttttt gatttgaatt gtccatgatt cgtaccgata
    34441 gcaatagcta atgcatccac gcctgtagct tctacaaatt tcactgcgtc ttccggtctt
    34501 gtatatacct tgtcattttc agcgacatgg attccttctt ctgtaccgcc gattgttcca
    34561 agttctcctt ctacggaaac accgtgcgca tgggcatatt ctactactgc ttttgtcttc
    34621 aggatatttt ctttgaaagg cagatgggac ccatcataca tgacagaagt atatccgctg
    34681 tcgattgctt ctttgatatc atcaaagtct cgggcgtggt ctaagtgcaa gactgcatca
    34741 accatttctt cttctgcaat agctgtcatt acattgacta acacatgcat gccgatatat
    34801 ttagcggtat cgacacttgt ttgaataatg atcggcgaac ccatttcttt tgctgcgcct
    34861 agcatagcag gaagcatttc taggttatgc gtattaaaag ctccaactgt aaaattcaac
    34921 tcttctgctg tttttgttac ttctttcaat gttgtataca tcttttattg ccaccttatc
    34981 tttttatttg tttcctttga tcgttacttg ttttgcaatt tgtcgcatct catccatttt
    35041 gcccatgtag taatcgatcc cttgatcatc accagcacga gcagcatctg cacgcttctg
    35101 gttatagagg ctcatcagtt ttttataccc atctccgatc gtctgtttat tttccaaact
    35161 cttttctaaa tattggatgg cttgatcgtt cacatacaat tcagaaaatt taagtccaac
    35221 ttgttcataa agtttcgcta atttatgctt atcttgggta gtggaaatct cttgtttcaa
    35281 ttgtgtaatc tctgattgaa tcttttcggt cgcttctggc gatagggctt tttcttcttt
    35341 tgccagaact tttttcggtt ctttttcttt tttcttaaaa aatccaaaca tcattttccc
    35401 tactttcaat taaaatgctt tcaaaactgg gcttaagatc caggcatata agagtgctgc
    35461 ggctactgta tctacatcgg ttgctgtagc attgatgaat cccatcgaat taaagatcgt
    35521 tactagcaaa gcgggcaata atgtaataaa gaaaccatga gcaataccgc caatgatcgc
    35581 tcctcggcgt ccgccaactg cgttgccaaa tatccctgct gtccctccgg caaagaaatt
    35641 ggttagcata ccaggtaaaa tcatcgctaa gccgaacatg ggcaaagtaa acatggcaat
    35701 aatcgtacca atcgttgtcg taatgaaacc taaaatgaca gcatttgggc tataaggaaa
    35761 gaaaactgga caatctaagg cagggatcgc atcaggaact aatttcatag ctatccctcg
    35821 aaatgccggc acgatttcac ctaacagtag acgtacacca gctagcaata catacacacc
    35881 cactacgaat tggatggctt gtaaaaaggc aaacatgata tagttttgat ctcctgataa
    35941 ttcagaagcg aatggttcgc ctgcaaataa aacagtgatg atatatagcg ggaccatcac
    36001 gaccatgaca gacaaataag tatcttgcag aaattcgaaa tgtgtcggca acttgatatc
    36061 ttcgatcgac tttttatttt cgcctttctc accaaaaagc ttagcaacac cggcttcaaa
    36121 taagtacccg atcgtacaaa aatgacctaa agcaatatca tttgatccag tgattttacg
    36181 aatgatcggt tgcgctactg ccggcatagc aatggcgaaa caagctccga taaaaccacc
    36241 gaccaagatc aatacgatcc ctcgcagacc ggcaaaatag ccaaagactg tcgtcatcgt
    36301 tgccatccat aaaattgctt gaccagttaa aaaaatgtac ttccattttg tgaaacgagc
    36361 caaaataata ttgaatacaa aaatggctaa gaacgtcagt gcaatatctc tacctaaacc
    36421 caaatcattc attgcttgac cattgataga ttcaatagaa gggatgatcc cttgcatatg
    36481 aaatccttca gtaaagattt tcccgaaata agttaaactt ccaacgatga tgctcgaacc
    36541 ggcactcaac acttgaaatc ctaggagtgt ctttaacgtg ccagagatga cttgtcctgc
    36601 tgatttcttc tgcagtagta gacccaacat agcaatcaat gcaatggtga tcgatgcctg
    36661 agtcaaaata ttttcgatga taaaattgat aacgcccatg tttttaccct cttttctttt
    36721 tgtatttaga taactccctt atcttgcaga actggggtga ttttttcttc aatttcattt
    36781 tttgaaacaa tatttttcaa atagatcatc gccgttttag gatcaactgc gaacttttcg
    36841 aactgcgaac gaaaattttc tgctgtgatg atcacatctg gtttcatgga aactgcggaa
    36901 gaaatatcac aatggtccag tttggcttct actcctaatt tgttcaaaac atcctcagca
    36961 gacatctgtg ctgcaaaact acttcctaaa cctgcaccgc atacgaataa aatgttcaat
    37021 ttgttcatct ttgttctccc ccgtatcatt tattatttct caactactga aaaaagcagt
    37081 tgtttgaaat cttcgatatt tgtgcattca atcaatcgat ccaacttctc ttcgttgttg
    37141 atcaattcaa ttagttcttt catgatgttc agatgcgagt aggagtctac tgctgccaaa
    37201 caaaaaataa tctttactgg atccatttca ggattaccga aatcgattgg ttgctcaatc
    37261 gtagctacgc tgactcctaa tttattgacc ccatcttctg gtcgggcatg ggctagtgcc
    37321 atgtgtttgc caatcacgat atacggtccg taatcattca ctgcacgaat catcgcttcg
    37381 acatagcgct cttcgacaat gttttcttta accaatggtc tagcagctag ttcaatcgat
    37441 tcctcccaag tagtggcttt ttctttgatc atgatattgc tgtccgtcaa aatatctttt
    37501 aacataggct ggatctccct tttgttgatt tctaaattgt ttgtttcgaa taatttttct
    37561 aacttgccat agatgattcg atctactcgg ccaccacttt ctttcatgac atctattatt
    37621 tgagtgaata actccgtcga ttcatgactg ttaggctcta accgctgtag ttcttcattt
    37681 cttttcaaaa agtcttctat aaccaagata ttcgtctcag ttaaaatcgg atcgacaact
    37741 aaaagcggct tatttcgata attcaattga aaggtgctga aaaccagatt gacatccaat
    37801 ttatctacta attcgatttc ttttgaactt aaaactgcag taatctcgat ctgaggaaac
    37861 ttttctttca gattctctgc taacagattt ccagtagcca tcccatggtt acagacaacg
    37921 actgatttat aaacataatt caagtctcgc ttgattacac tggcgatcgt cgaaaagtgg
    37981 atgacaagaa aagctatctc gtcctccagt atcttgccac caattacttc ttcgatcgta
    38041 ggaacaaaat gagtgactgc gcggtagatt gggaaataat ttgttcggat gttttctttc
    38101 aatggattca tgacttggat atgattcttg attcttacaa tcagtgcagc catgtgctta
    38161 tagagatttt cgaataaagc ttcttccttt agatggaaag gtacgtgtgt ctcgttctct
    38221 acaaattgga tcaattggat cgttagtaat tgagcttgta cccactcgat tgaattggag
    38281 atatctctag cattgaacgt ctcaatagtg aaacgaagat aatttctttc tacttgcggg
    38341 acattgtcca attgaaattc ctcgatcatg tggtcaataa atgcagaaaa gctactgtct
    38401 tttatttctg tatttttcca attaatggca gagataaatt catgtttcct gattcgctgg
    38461 atccagatca atgtaaaaag caggatttgt tttttataaa tatcatcttc ttgtttcaac
    38521 aacttattgg aatagatctc ctgtattttt ttgatttcca ccaaaggaaa ataatggaga
    38581 aagatttttt gagccggtgt taattgtgca tcttgattca taaagaagtc ggtcattccc
    38641 agattctgat tgataaaatc atagatcatc gtacggatag atcgttctga acctttataa
    38701 accagtcctt gctttccata actgacgatc tctatcccat aactgaccag tgttgctcgt
    38761 aaccgtctca tatcttcatc taatgtactt ttagagattt gaaattcttc ttcttttttg
    38821 tttagatgga tcgggattcg gctgtaagca acggctaaaa ctagatcaaa aatacgttcg
    38881 cgccgattca aaaagccgtc tttcacatct tttatttcag tttctaatcg agcttgcttt
    38941 tccttttcca gcttcaacaa aaaaactttg ttgcgaattg tacggatctc cggaagcttt
    39001 ttactgctca accagatatt cgcttcaaga acttcctgac ggatcgtccg ctgactgaca
    39061 ttgaactcac ttgctagatg ttttgctttt actggatatt gggctgtcat gattctgtga
    39121 agtatttcaa ttgttcttgt tttcatagaa atgatctcct tctgatttca agtatatggt
    39181 tccgcttaca taataaaaag tagatatttc tgccacttga agtggaaaga aagtaagcaa
    39241 agcaaacaaa aaataagcca tatgcttttt catctcctca acgaaaaaga catacgccta
    39301 ttattacaaa aattttgccc atctatacaa aaaagaggtt gtgaaaaagc tgtcctgcat
    39361 caagtaataa gaacaactaa actaaaatag tgcttcctat ttttcgtttg gttgttctta
    39421 ttatcgcaga tgcaagcttt taaacaccat ttagtggaga ggttgtgaca gagatgttca
    39481 gccttgagcg ataagaagcg attttgaaaa atcgcttccc atattttaca aaatcgtgcc
    39541 ttattgccga taggctatct ctggcacact gtttattcag aaaaaggagc tgcgacaagg
    39601 cttttgtcac aactccgttc ataaatattt cctgtcaatc cagcataatc ggctgaataa
    39661 aatcggcttg ccatcttttt tctttctcag aaaaatcaaa ataccatatc tgagaaaaat
    39721 actgtcgttg tgtcttactt cctgagatca atcgcccatc ttttcgatcg actgaataat
    39781 cacagcatct gaaatcaatt cggatggcaa aactattttc gtggatctgt cgataatttc
    39841 ctacttcctg aatagttatt tttttagtat gatttcttac accgtctgct ttgttttttt
    39901 gcagaacttt ctgatgttcg gcaaacagct tgtctgtata acagcttctg gctttttcag
    39961 gagtttcttc ttcccaagcg gattggatct tcatgaatat cttttggatt tccagaaact
    40021 gtgctttctt ttctttagcc gttcctgaca tgcgggacaa tatctccgct ttcagccttt
    40081 tcttttgtct tttattcttg acgatagaaa aagcagaaac ccctcctact gacaaaacag
    40141 ctaatgcaag aaatcccgtt tgctcataac tagaaggacc tgtacctgga ccataatacc
    40201 cataagaatc tctgccgtaa gaacgactgc caaaataact gcccccatat gaacgaccac
    40261 caaatgaacc tctgcccgaa cttcgaccgc tagatgtacc tccaccatga ccacccgcga
    40321 cagcgaatcc ttgggtcggc agagcaaaca aacataggcc cactaacaaa atgattgcta
    40381 aataattgat ttttttcatt gtcttcctcg ttatctttta ttacaaaaaa ctatttttat
    40441 gatagccttg ctaaaaggaa gagtcaataa ccagatgcat aaactgtttt tttagcaatt
    40501 ttgtcttatt tagtcattga gtaacatcgg acaaatgtat gtgtcggcag tccataattc
    40561 ctctatcttc agcatgaaca agcttgcaga caagagatct gcgcttcctc caggagagat
    40621 ttggttttcc tgacaaaagt gcaacagtgc tgtaaatgga cgatttggta aagcactttg
    40681 actagcagca tgagcaagtt tttgcatttc cactaacgtt ttctgatcac tgcgatgcaa
    40741 tacgcatgtg tcatctagcg aactcatcaa aactaacaac atatgcattt gtttctgtac
    40801 atatgaatca ttatctagct ccttagctgc ttggattgca agcctgatat gcggataccc
    40861 taatatcgct tcttcatatg caccatttac gccatatttt tgcttcaccc tatgaccatg
    40921 agtggttttt ttatgacgat attttgtatc aggaaaagca gcaagcttgc tgacatcatc
    40981 aagtattttc gataaagaaa aaggctgttc catactgatt ttttgtgcaa tgacactgat
    41041 caatagcccc attgcccaaa tcgctccttt atgggtattg atgccatttg tagcaatcag
    41101 cattgccttt tccgcttttc gcccaatctc agcaattctt tctcttaact cctgatcaat
    41161 agcatgatgc catgctattt gagccatttc ttcaaaaaaa ggagttagcg tcttagcaga
    41221 cttaagaaat aaagcatagt ccatatcgga atgtgcaccc gcatccagtg catccactaa
    41281 tccaggtttt ggacttaatg ttacttctgc gatcaatgct tcctctacca acgcagcgag
    41341 ttgctgagtc actgcttttt ttcgttcgtc atttatcatg acactcactc cttcttccta
    41401 ctattacttg ctgaaacctt ttttgatagc tacccaaatg atatgaagta accagcttaa
    41461 agcaaatgca caacttgccg tcattagata agctacaaga atcatgactc ctgaaggtaa
    41521 ctgcagccaa ctatctttaa acaataataa cagtacttgg aaaacaaata cattggctaa
    41581 atacgtacga taagcataaa cagacaacca tttgaacaat ggcaattttg gcgaatcgtt
    41641 tacaatcatt ttggaagcgc ctatgaagac agcaaagatg atgactaaac tgtataaact
    41701 ttggatcgta ttcaagtaag gtgcattcgc aaagctcaag tctcctggat gtgcgagcaa
    41761 tgaacggaca gaaaagaacg cgataactaa ggcaacaggt aagaaaagat aacgtactct
    41821 gtccaaatat tgatagactt tttcgtgata aacaaatgcg gctaccccat aaatcatgaa
    41881 actagcaaca aaccgatcta ataagtacca gctgtcatga taaggcccag tcaatacttg
    41941 tgtttgatag aacagatacc atgcgacata taaaatcgtt gaaccaatca atactggcca
    42001 tacatatttt ttattagcaa atactttgta accgagccaa acaaaaaacg gcatgaacaa
    42061 ttggatctgc agcatcataa cggtatacca taaatgaggc gctccatcac ctgtgacata
    42121 tttcagtaaa aagcttgcag tatccgtata cggagtttgt tgttggactg ctggaaatag
    42181 ccaaagataa gccgtcgtcc atagaagata cggaacgact agttcaaaaa acttttcttt
    42241 caaaaattcc caataagaca aatgctgact tgtttttacc atattataaa caattgcaaa
    42301 aataaacatt ggtgctgtgt atttaacgat gacgtaaaag gtaccaagaa aagctgtatt
    42361 tgttgcgctg tggctgtttg ccaaagcaaa gctcaatatc gtttgcatca taacagccgt
    42421 tacagctgat actttctgga agtcacttac gttgattttg atttttttgt tttcaaacat
    42481 ctctttctct ctctttttct ttattaccaa agttctttgg ttaatttagg tccttcgact
    42541 gttttcaata gaattttttt ctctggctct ctcaaatact cttttagtga aaaaccgcct
    42601 tgccgagtca ctacttgggt atcgaattgc tcaaaaaaag caggatgcga ttgaattacc
    42661 tgaattggca attcaatcgg tgaatttgcg tacagcagca aatcaaaatc actggtttct
    42721 tttactgccg gaatcccagt cgataattca aatcctaagc tgccgccgat tccccattta
    42781 catcctgata atatgtttct agcagcatga aattgctgat aaacaggata atccatgatt
    42841 ttctcttgtc tatacgattc ttgttcgatg atgtcccacg gatgaatgac ttgtttgacg
    42901 gcatgttttg agatttctgt tgaccatctc tgattgcggg caggtccacg taaaccaatc
    42961 ggtagtattt ctgcgtgatc cgtcgacgca cgacgaactg ttccgaacat cttttcagtt
    43021 agaacgaccc attcaggcaa ggtgaaccct tctatttcat gtttatcaaa aaggacaata
    43081 tcatgtgctt gaatcatttt ttatgcatcc cattcttcgt ccattttggc acggacttta
    43141 tttgtttcga cacgtccgaa tgttttggct gtttcgtttg tgtatcgatt tgtcagcatc
    43201 gtgtcatttg tattgcggac atcttgaatg gcttcattga ttgcttcgat gactttgctg
    43261 acattttgtt ctgaagcagc ttggtcggtg atatcttcta ggaaatgata gatcgctcct
    43321 agttttgtat aattattgat atcgtaagcg atagctggaa ctttttcagc agctgcttcg
    43381 atttcagcaa ccgaacgttt tgtcacacgt gcagcacttt ctttagacat cgcttggacg
    43441 gtaatttctt tattgtttaa agcaatcaag cggcttgatt gtaatccatg tgctaaaaat
    43501 gcacctgaaa tagcatttcc tgcaatcaat ccaataactg gatgtccagc ttgacggagt
    43561 tttgcataag cagcagcact attagccaaa gccacatgga taccgatcaa ttcttcgtta
    43621 tagccgtatg cttggcttgg tacatcgatc acaacgatga ttggccgttt cacttttttg
    43681 tctttatctt cttcgtaaac gtgatccaaa atttgtccca tgcggaaacc ttcaactaaa
    43741 ccaacttcac cgtttcgtac acggtacatc gggtttttag gatcaggtac gattgatgtc
    43801 acgatggctt cttctatgaa tttacttgat ttaccataat acacagtact aatagaagaa
    43861 gttggatttt tcatgccagt tagtgccgca aaccaatcgt aaccaacact tgctgcagct
    43921 ggttcatttc cttctgctac ttctggtaag tcaattgatt ttgcttgatg gttagcaatt
    43981 gcttcattgt aatcgtcaat ggccattggt tttgaaagat ctaaagaatc aagtaatgat
    44041 aaataaaatg ctgagctttc actgcgatgc gtgtcttttc gttcgtccat tgctgcgatg
    44101 acagcagctt tgattgcttc tacactatca gtcactaatt catcaatgat ttttgattct
    44161 acccgttgac gagatcctaa tgtattccaa atcaagtctt tgtcactaga atcaaattct
    44221 cgaacgcctg cttcttgttc aataacttct ggtccattca aaccgactct cgcttttttt
    44281 gttgcgatta aataactcat taatgctgaa gtaatcgaca taccgccaaa tgatcctaca
    44341 cgtcctggaa tcaaaccgat gactggtaca tattgtttta gagcaatgat tacatttccg
    44401 atttctgaaa tagataacag tccgtagttt gcttcttgca aacgaacacc acctgtatct
    44461 agtacaatga ctggaaagat ctcatttcct tttttatttt cttctaacac atgttcaagt
    44521 gcagcaacga tttttgcacc ggaaacttca ccgataccgc caccttggaa ttttccttcc
    44581 atagcgatta ctactgcttg tttgccattg atcgtgccac ggctgacaac gatcccgtca
    44641 tcactttcag ggacgattcc ttgtgctggt aaacttggcg cgatgatttg atcgaatgga
    44701 tcaatcaatt cacgtccatt gtcaagtaga gcgttcactc gttctctggc gtttaattct
    44761 acaaaactat ttttcatctg aaagttcctc cattgcttgc gtcaatcgta ggtacacaac
    44821 tcctggtgta gcaccgaagt cattgatcac gatatctgct gaaattggat aacgatcaaa
    44881 aaatctcgtt aatacattgt tccatacttc gccgaagcca tcgcttcctg tacaaatttt
    44941 tacagtagat tctgtttttt ctgttggaaa catcaagatt tccaaatctc ctgaaccgac
    45001 cactcctaca tggattttct tattgatcgg ctgtttcgtt ggatatttat aagctaattg
    45061 ttccatggat ctctcccctt tttgtaaaac agagcataca cgttctgctt attcgtcttt
    45121 cttcttccat ctgggtaaat ggcgacaaaa ggtttttctc gccaatttta tgaccaagtt
    45181 ctgaatttca ttggtggttc atacaggcca tctgaccatt cgatcaaatc atcgatggtt
    45241 tgtgcagcta ataatgaacg tttcgcttca ctacgtttga tgcctagatc ttctggtaat
    45301 ttcacgatgc cggctgcacg caagctatct agtgattctt tcgtgctagt cattccaaga
    45361 ggtgtaacac cgccaattgc tgcaattgct gcttgtcgtt cttcttttgt ttttgctttg
    45421 tacaaataag caattccttc ttctgtcacg atatgtgtag tatcttcgct ataaatcatc
    45481 actggtgcat tggctaagcc tgcttgtttt tgaacagcta gtgcatctaa ttcttcgaca
    45541 aagactggtt ttttatttga accatatgtt tcaaccattt gaacaactaa tttttggcct
    45601 ttccctaatg gatcatcatt gtcgcgaagt gattgccatg ctggtgtaga atgacgacga
    45661 ccgccaggat tatgtcccat gttaggcgca ccgccaaaac cagataaacg tccttttgtt
    45721 acggtagaag agtttccttc gtaatctaat tgcaaggttg aaccgataaa catatcgatg
    45781 gcatattgtc ctgcagcttg tgataatgtt cggttactgc gcatcgtgcc gtctttaccg
    45841 acaaaaaaga catctgggcg agcagcaata tatttttcca taccaacttc gccaccaaaa
    45901 ctatggatgc tttcaaccca acctgattcg atggctggaa tcatcgttgg atgtgggttc
    45961 aatacccagt tcttcgcgat tttccctttt aaccctagtg attcaccata agttggcagt
    46021 aacaattcga tagctgctgt attgaatcca ataccgtgat tcaaagattg tacgccgtgt
    46081 tctgcgtaga ttcctttgat tgccatcatt gccattagaa tttgcaattc tgtgatgttt
    46141 tgaggatctc gtgtgaataa agcttctaat tgataaggtt tatctgcttt cacgacaacg
    46201 tctacccagt caccagggat atctacacgt ggtaaagaat cgacaacttc gttgacttga
    46261 acgatcacga taccatcttt gaaagctgca gcttcgatga ttgttggtgt ttcttccgta
    46321 ttgaaacctg tgtacaagtt gccgtcttta tctgctttat ctgcagctac taaaacaaca
    46381 tttggaatca aatcgatgaa taagcgtcca taaagttcca aataagtatg gatatcacct
    46441 aatttcattg tgccgtcttc aatcatttgg gcgacacgca agctttgtgc accagcataa
    46501 gagaaatcga tcttgcttgc gattcctttt tcaaaaatat tcaaatgttc tggtcgtgag
    46561 atactagaca tgatcaaatg cagatcattc actttttctg catctacttt ttcaagactt
    46621 tctgaaagaa aacttgcttg tttttgattg tcgccttcta atacaacttt gtcgccggga
    46681 gcaatcagtt tttccattac ttctacgatt ttttcagttt ctacatactt gccattgagc
    46741 aagttgctga ttttttccaa tcgctgcgct tttttttcgc gtttagaagc ccagttgcgg
    46801 tttggttttt ccataattag tcatctccat ttttgatata gaaacgtggc gtcatcaata
    46861 ccatgttggt ctagattgat cgtacggatc cctttatcta caccaaattt tgtatgaatc
    46921 agctttgtca aagtattgcc ggggggaaat tcaatcgtca gatccattcc taattctttt
    46981 gccaagtcca tcatttggtt ccactgcaca gggaaagcta ggttttccaa caaatcttta
    47041 cgaatctctt ctgcgtcctg agtggctctt ccatccgtat tttttatata caaacatcta
    47101 gcatcagata gatggacaga ctgcatatac ggcttgaatt tcttgaccgt atcattcatc
    47161 aagagacagt gagatggcac agggactttc agcagcttcg ctgtttgcgc ttggtgccgt
    47221 ttggcttctg ccaacgtaac ggataaacct tcgatactcc cagataacgt atgttgcatc
    47281 ggacaatttt catttgacag ataaactgga tggtcaggat caaatgtttt tgctacaatt
    47341 tcctcagctt cgcttctcgt cacacctact actacgccca ttgcataacc gcttggataa
    47401 gcttctttca ttaagactgc gcggtgatag actagttccg ctgcattctc aaatctcaaa
    47461 gatcgacaag caacagctgc acaaaaagca ccaatcgaat gtcctgctgc aatatcaggc
    47521 tgaataccgt ttttttccat ttcatctaaa taaaatactg cttttgtcag taatgctagc
    47581 tggataaaaa ccgtatcttg atagttttct tctttatcaa ctaaccgata ccccgtcaca
    47641 ttttctaaac gttccatgta catttcaggg atatccgcta acatccctaa cttctgactg
    47701 ccttgtccag gaaataagaa acctgtactc atgagcctcg cctcctcagc acttactttt
    47761 tccactgcta tcttagtcaa tttctgctat aataaaaaat gccaaaaaaa gaaaaaacca
    47821 taacaaaaag gtatggatgg gatggataat tatgaaattg cttcatctgc agtattttaa
    47881 aaaagtattc gaaacaaaaa atgttaccca agcagccaaa gaattattta tttctcaacc
    47941 tgccttgagc cgtgcgatca aacatttgga aaacgaatta ggtgtccctt tgttttacca
    48001 tagcggcaga aacattgaga cgaccgttta tgcagaagaa ttttatccat atgcagtcaa
    48061 gacattagac acattagaag aaggaatcaa attattgtct tcggtcaatg agaagatcgt
    48121 tacttccgtc atattgtatt tagaggtagc ctctgtttct attccgaact tggtccgaat
    48181 ttttatggaa aagcatcctg atatccaatt aacgatcatg caacatcatt taccagatga
    48241 tattaccgat cctgtactgt acatcacttc ggagatgaag cctggcatga ccaatattcc
    48301 tatcatgaaa gaacctgttt atgtcgccat tcctaagaaa catcctcttg ctaaaaaaga
    48361 acatctgcaa ttagaagata tccaacgcac ccccatcttg atgctgtcaa agagcaacgc
    48421 cttccgtcat acactggatg cagcacttga agcgaaaagt atcgaattga cgatcagttc
    48481 gacgacagat gatcctgcga cattacgttc tattgtcaag caaggattag ctgttagctt
    48541 tttcccaaaa gtttcttggt cttacgataa aaatgatcct ttcgtcttgc gggaaatcaa
    48601 agatttgccc ttgacacgaa caatttattt atcttctagc ttcacagaag atcatccttt
    48661 gatcaaaatg attgcaaaaa cgatgcggga attttattta ggaaaataaa aaaaaaagag
    48721 ctgggacaaa attcttgtcg cagctctttt ttcgaacaaa cggtgttcca gaagtaattt
    48781 cttagaaaat aagccgaaat ttcccaaaaa tttgagaagc aattttcgta aattccttct
    48841 tatttcttga agctaaacat ttctgtcaca acctcttctc tattgtccta gacattcatt
    48901 gcgttgattt aaaacttcca tcaatcaaat ctgctagttc atctagcttt tctattttac
    48961 gtagatcaat agagatccaa tgttctttat tcatgtgata ggctggaaaa tacccctctt
    49021 ttcctcgcaa aattccgcca agttcaggat cgaccttcaa atcaagaata ttgatgatcc
    49081 tatggtcact tgcatcatca tagatttttt cttctggaac agacatcagg accccaaacc
    49141 attttttgct tttttgatgt ctgaatacgc aataatcagg aaatttttta aaaatatatt
    49201 ctggagacaa attgtatgtt tcattgatgt gatcgattag ttcttttttc gttagcatct
    49261 gcttccatct ctttcttatt gattcgcttt attcaaagat accacgtaag aatagaaaaa
    49321 tgaatacaag tagttgctaa tcaaatactt cgatattttt cttttttatc aactcttaaa
    49381 tgttcaatcc gcctgtgttg gtcttgctac catctactat taaaatgttt attattgttt
    49441 gcccacctat tttttgattt tagtggtatc atttttcaag ttcatgatga aattcttatc
    49501 atcagtaaat gctccagaaa aataaacaat gattgaaaaa tcagttgaaa cttgaccacc
    49561 gtttattttc tccgtattct tagaatagaa gggagtctga ttgatgtatt cactaatgaa
    49621 aaaaatcatt accgaaaaag atattcgacg tcatgtttct ttagtagaac aattgctgaa
    49681 tcatacaaag ataaccgtaa atgaattagc tgaaatcata gggacaaccg aacgaacgat
    49741 tttttctgac cttcaatcta ttcgctctca gctgcccgaa ggctgggaca tcatcagcga
    49801 tcaagcagga atcagtttgc aaaaccagca aaatttatta acaaatgacc tttgggagat
    49861 ttttttcaaa caatcagtca gtgtcgaact gctgaaaaat ctgcttttta caaaaaaagt
    49921 tgctgtccct gattttctag ccgaccatgg gctttcttat gggacattga aaagacatgt
    49981 gactaaaatc aatcagcgtt tagcaagtta cgacttgcag atcgatttga cgaaatatac
    50041 agcttgtatt cttggaaaag agcgtgcgat tcgtactttt tatcatcgat tgctgattcc
    50101 tttcactcat aacaactatt ttttcgagga ttattcgatc catgaatccc attattttca
    50161 atttttaaga aacctcagtc aaactgaact agcggttgaa acagaagaga ttttcggaac
    50221 atgttggttc tttatcaata cgatccgtat aaaagcgaat tgccgattag attcttctat
    50281 ccataccaac agcacactct cctccctgta tgactctgca ttgaaaaaat tgtatttaac
    50341 ggaaggtatt tatcttaaag acactgagct ttcttttgct tctttctgct ttctggaaag
    50401 ttggaattac aacaataact atggacaaga aaccgctcgc tgcctgcatc attctccatt
    50461 tttagaggtc ctcgaaacgt ttgtagagga actagcttca gagctgtctc ttgatcaact
    50521 aaaaaaaaca tctctgactg ataacttggc tctcctcata ttaaaatatc atgaatcgcc
    50581 gatattgatt gaagcattgg accgtcaata ccatcgtttt ttagaaacat atgatcagca
    50641 atatgcacac ttgtatcaac agaaagagaa tttactgaac gatctaaaaa aagaaataga
    50701 aatcaatgaa ccaaattact ttctccaact cttgtctcta ctgatccaac aaacgatttt
    50761 ttctatcaaa ccgaatttgt tcaatgtcta ttttattttc caaggagaac cttcatggaa
    50821 ggcgttcttg cagcaggaat taaaagatta cttaggaaaa cgtgtctgct tcttacctat
    50881 cgaactaacc gatctttcca ctatttcttt tgaaaaaaca gatatcttga tttccaattt
    50941 ccctctagat tctctagata tccctattgt atacatttct tctattccaa cgaaaaatga
    51001 gctcaatcgt ttgactgaac tcactttcaa aactttttta tagtaacggc tagcgactgt
    51061 attctctagc tgttttttct tttttgcgaa acagtttgtt catgatccct ttttctattt
    51121 ctacaactaa gaatagaaga atcacatttg ctccaatcaa taactgttgt gtcatcgtta
    51181 aacttactgt accgatcagc tgctggccga ctggtagaaa aataaccatc ccttgcaata
    51241 aagcaaggat acctaatgaa agaaaaagcg ctttgttttg caaaagacct tggttcaatg
    51301 acggcttgga caactctctg caattgatca tgtaaacagc ttgagctaag acgatatttt
    51361 gcagaagaat ggtttgctgt aagctttgcc cttcaaactg catagccaat aaataggcag
    51421 gaaccatgat cagtagggag acatagatga tccgaaacaa atgataacca gataggattc
    51481 cttgctttgg atctcttggt gggcgcttca tgatttctgg atctggcgac tcgaacccta
    51541 gtgcataaga taaagtgatt gtcgttacca tattgaccca taaaatctgt actggggaca
    51601 aaggtagcgg gcgattcagc ataagtgccc aaatcaagat caatccttga gctaatgcag
    51661 ttggcaagaa gaaattgatg gttttttgca aattatccaa aatccgccgg ccctctttta
    51721 cggcttttgc aatcgtatgg aaattatcat ctcctaaaac catatcagct gcctgtttgc
    51781 ttacttcact acctttgatt cccatagcaa ttcctacatc ggatcttttc aaagcaggtg
    51841 catcatttac gccgtctccg gtcattccga cgatttctcc atttttctgc aaagctttaa
    51901 caattcgcag tttatgttct ggtgtcgttc gagcaaagac atcgactatt ggcacttgct
    51961 gaatcaactc ttcatcagac ataagatcca aatcgatccc ttctaaaact ttggcagtat
    52021 gttttaagcc aatttgttcc gcaatcgctt ttgctgtgtc tttgtgatcc cctgtaatca
    52081 ttttcactga gatccctgct tgttggcatt cctttacagc tttgactgca ctttcttttg
    52141 gcggatcgat caatccggcg atccctgcaa aagttaattg ggttaaagtc tcatgcgtga
    52201 gttcctgttt cgaatctact tttttataag caaatcctaa aacacgctga ccttttctcg
    52261 caaattctgc agctgtctga ctccatgctt cttgttctgc agaagaaaga gttgatttag
    52321 caaataatac ttctggtgca cctttgacgt aaatcaccgc tcctttttcc tccgcatgca
    52381 tcgttgccat atatttgtat tcagaactga acggaatctt tgcttctatt tttttcaaag
    52441 gtagattggc tgtttctgtg tgtcgaatca gcgcaacttc tgtcggattg ccacttaaat
    52501 tttctgtttt ctgttcttct tttgttttta attcctgaca gtttttcata atggacaaca
    52561 cgcaggcttc ttcttttgca gctacatcca caactgtcat ttcatttttt gtcaatgttc
    52621 ctgtcttatc tgaacagatc actgtcatag aacctaatgt ttctaccgaa ggaagtcctt
    52681 tgatgatcgc cttttctttt gccatttcat gtactccaac ggatagaatc atggtcaaaa
    52741 cagctggcaa tccttcgggt accattgaca ccaccaaagc aatcgtagcc gaaagaagga
    52801 tatgccaatc cataccatat cgaaatgtcg taaaaataac taagaatagg ctcaaacaaa
    52861 gaatgccttg aaaaatttgt ttgtttaatc ggttgatttt tttaatcaga ggcgtcgttt
    52921 gttgttcgac cgattgcaat gcttgattga ttttaccgat ttctgtcgtg tttcctgttt
    52981 ctacaacgac accgactgca gaaccagatt gtacaagcgt tcctgaaaaa gccatatttt
    53041 tctggtcgcc gatcgattct tcttctgtca aaacggcatg acttttttca gatgcttctg
    53101 attctcccgt caaaatcgat tcttccacta ataaatcatg tgcttcgatt aatcggatat
    53161 ctgctggaac gacatctccg gcacgtaaga caatgacatc tccttttacc agttctttcg
    53221 cagggatttt ttcttgcatc ccgtttgata gtactatcgc ttcttgtccc atcagctgct
    53281 tcaaaccatt caatgattct tccgcttttc tttcttgcca gtaactaacg agtccattga
    53341 cgatcactac gaataaaata atacttcctt caatatattc actagaagca aatttcaaaa
    53401 aagaggcaat gatcaacacg atcatcagca gatcagtaaa atgctttgct atttttttcc
    53461 atcgttttgt ctgctgcctt gcttttattt cattatatcc ttgatcagtc aggcgtttct
    53521 gtcgttcttc ttgcgacaag ccttctgttg atgtttgata gtttttcaat acatcttcta
    53581 ccgtcaatct gtaccagttc atcctatccc ctcctgttta attgatactg ttattatacg
    53641 cagggaagtt ttttcaggcg tatcgattat ttcagggggg tctgaaataa ttcgttttgc
    53701 aaaatgtatc tgggcctaac tgatatttct attgcttatt cttttatata caaaatttaa
    53761 gaatacaaac cagataacca ataaaaaagg aataaagcgt gtcgtgcttt cgaataacaa
    53821 gctgaaagca attgaagtaa aaaacaccaa caaagcaaga tctttatatc tttgtgtagt
    53881 gttccgtttc ttttctttaa taaattgaac atgtgcacac aagatcgaag accacacaaa
    53941 tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa aacaatcgac tatcagctac
    54001 aaaaaaatgt agacaaagac cggcaaaaaa taacattcct gtaattaaaa taccgtgtaa
    54061 aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc aaactttttt cttttgctgt
    54121 ttgatataaa atcctgctag tactgtagac tgcactattt gctgaggaca aagaagcaac
    54181 caaaatcatt agattgacaa ttgctgctcc gccttggcaa tggatcactt gcagcatagt
    54241 cactaaagga ctttctttta aatctaaaca atgccatgga acaacaaata aaatcgacaa
    54301 aatcgttcct atatagaaaa agagaatacg ccaaggcaga cagttgattg cttttttcaa
    54361 attcttttgc ggatcacttg tctcgccaac ggttaagccg atcatttcaa ttccagcaaa
    54421 actgaataaa accataggaa atgagactac cattccagca aagccaaacg gtagtaattc
    54481 atcgatctgt ccaaaatttt ctttgattcc gatagactgg gtatgaaaat ctgagacact
    54541 caatcctata cccaataaga taaaaaggaa aatccccaag atcttgatga ttgagaaaaa
    54601 agattccaat tcaccaaacc acttgactgc aacaaggttg actgccatta agatcattag
    54661 tgcgctacct ccgcttatca aagctggtac atgtacaaac cagaaatgga tatacaatcc
    54721 tattgctgtt aattccgcaa gtccagctcc aagccagcaa agccaataag tccaattgac
    54781 aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa tcactgatag agttaaatgt
    54841 actatttgct aaaaataaat cacccatggc ccgcatcaaa ccataaatag ccaaaccgca
    54901 tatcaaataa acgagaataa cagctggccc tgctgatgca attgcctttg ctgaacctaa
    54961 aaataatcct gttccaattg cgccacctaa tgcgattaac tggatatttc gattagttag
    55021 agtccgtttc attgccggtt tttccatcaa gttcaccttt ttctttttca agcagcatgg
    55081 cgacttcctg ttcccaagaa ggtttcttta ttttttcaat acagagtggt actacaaaca
    55141 aaatgacact gatggctgtg actgcaatta gattgatcca tgttccagct ttcatcgtca
    55201 gtccgacaga aaatccgatt gccgcaaata aaacaataac gacagtccaa actccaagat
    55261 tgctcttttt ctcttttttt ccaattctaa atggtcgatt caactttgca tgtgtataac
    55321 gcaatctcaa aactgcgatc cccatgatga catagacgat acagtagata acagtagttg
    55381 aatttacgag catcaaaaat gcttgattga cgccaggaat cagcaaataa aaaagtgcaa
    55441 agattgagat aatgacagac tgagtcaaaa taacggattt ggatacgtca aattgattcg
    55501 tcttccaaaa attgaatttt ggtggatacg caccttttct agcactttct gtcactgtct
    55561 ttgctggtcc tgaggcccaa gcagataatt ggactgctac accaatgaag acaagtaaac
    55621 taaacagatt cacaataaga tgtggcaaac caagaatttg gcaataaatc gaaatagatt
    55681 gcgcgatatt attcaattcc atttgtcctt ttggtacgac atttgctaca actaaagcat
    55741 tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa tactccgcga atatacgttt
    55801 taactggatt ttccaatcga gtgatataca cagaggacat ttcgatacca gtaaaaataa
    55861 acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt agtatctggt attaatttgt
    55921 cccatgaaaa actgcctaat gttccggttg gtacgatccc aactttgatc cacgcagcaa
    55981 accctaataa gagcatcatc gtcaaaggca cgtacaatcc taaccaaaca ccgacttttc
    56041 cgccgatttt tgccatatca aatttcaaat tcaaaagagt gatgatccaa taaacgacta
    56101 aaatgacaac taatgtaaat ttgctgttca aaccaagcgg cacattatcg ataatattac
    56161 caaacaacgg agcgagtgct gaagcgacca tcaccatccc aggaaacatt tggacccata
    56221 acagccatga gacaacgaac ccccattttt ttcctagagc attcgatacc caaagttctg
    56281 gaccaccttt ttggggaaac attccagcaa attcaccaga gatcaatgag attggaaaag
    56341 cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt ccagctcgtt gctgctacat
    56401 ctggaatatt tcgtacactt gctacaaggg cgcaggtcat tccgataaag gtcatggtcg
    56461 tgatttgctt tgatgaacca gtatctttca tctttattcc ttctttcaat agcgacatat
    56521 gtttttgttt tcttttattt ttgttcaaga acagaaatga caaggtttca tcgattcagt
    56581 acaaaaccca gtatcaccga aacatccatt gaacgatctt agtgaactgg gtttgataca
    56641 tgaaacgaac gatccaactt atagaacgtt ttataaaaaa gaatatcttg gcgaggaata
    56701 tatgaaaaac agggggaaat acaattgttt tgatgaagca aataattgtc tgttctccat
    56761 atgtctcttg ttattctttc acacaaaaat cagttctatt ggatgatttc gtttagtttc
    56821 ttttccattg ctttggcgat tttttttgta taaaaattga aaattcgatc atcattcaaa
    56881 taaggagtca tgatagccgc acgtagcaga gtgattttct gttctttttt ccattcttct
    56941 ttcgttagac ccattctttc aacaaaacga atcggagaat caccgtaatc ttcttgtgta
    57001 aatgtagtat gagaagtaat aaaccgttca ccataaacat cgccatccat ataagaagac
    57061 cgatcgaaca ttttttcatt taattcattg atagcgttca aatctgtaca gccttgttct
    57121 ttgaataccc aattgaccat attaaaatca ggatgattca atggatagac ttcgattgtt
    57181 tttcctttaa cagtaaaagt cagatgatcg agaaattctc tgaatctttg agctgcctcg
    57241 attgaagccc caatcaattg accatatccg gtcacattca atggcaatac acgatgtgct
    57301 gtccagactg cggcggcggt tgctccagct tttgatcctt ccaaaatata agctcctaac
    57361 atatctggag cctttactga tttttcaaac acatatggcg cgaaataaga aatgatattt
    57421 ctcatatttt tatgtttgat tacgattcct ccagcagcat aagggacata gcccatctta
    57481 tgaggatcta tcgtgacaga atccgcttct gaaatagctc tgaacccttc atatacctct
    57541 ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt caaagaattc agctaaggaa
    57601 gcatacggca caaattctcc tgcttcgttt agaaacagtg aacgcgcata gccgccatat
    57661 gcggcatcaa catgtaaata gaaatagatc ccttcatctt ttaacctttc tcttaattgg
    57721 acgattttat ctacactgtc tacttgacct tcttctgtcg ttcctacgac agcaaccaca
    57781 cctagaattg ggattttttg tccagctaat tcacgaattg ttttttccaa agcattaata
    57841 tccattcgat aatcctcttg aactgggata gcaaccatct gatctaagcc gactccgcaa
    57901 atatctaacg ccttcatcca agaatagtgt tttgtttgag ggaccagcca tttccctaat
    57961 ctttggatat tttttccgct acgggaagaa gctgctttga cctcatccat ttcttcgtcc
    58021 gtaaagcttt cggtcatttc caaaatttct tcaacagata aattcaaaag ttcccactca
    58081 gacatttttt tcactttttc aggcagcact tccttgacag ctaaaggaat cgatttgatg
    58141 caacgggcgt accataatcc ttcaagatta gcaatagaac catctgctgc aatgtgtccc
    58201 catccgtctg tcatattgaa taatgaagca aaatcctgtc ctacttctgc ttccatttgc
    58261 gaagttgcca tagaagattc caatgcaaca ttatttgggt tccacagcat cgcatagttg
    58321 taagctaata aagcaggcat caatgtttca gcattcattt gtccccagta acggcctgct
    58381 gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga cttcatccaa gacctctatc
    58441 atatggtcgc gggtggctag ataatctgga gtgagcttat ctccttcgct aattgcaggc
    58501 atatctgaag gaatataatt ttctctccat ccaagatgtt catctaccat cttgttcaac
    58561 aacattttat aaactggtcc attttcagct ttgtcgccta taaagacggc cttgatatcc
    58621 atatctttca tttttcttcc ctccatattt tgttggtcaa tgtcttgcag atacatgtgt
    58681 ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga aatttttttt tataacatca
    58741 aatccacgta gatttttaca aacaaaaata aataaagtcg gatttttctg ataattcaac
    58801 gcaaaaaagc gaacaatttt tcaataaatc agtcttctaa aatagataac ctaaaaaaag
    58861 cgaacaaaca aataaa
//
LOCUS       DARDHA010000003        53351 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000003 DARDHA010000000
VERSION     DARDHA010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.003"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            41..898
                     /locus_tag="SDA10_000117"
     CDS             41..898
                     /locus_tag="SDA10_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEG4239052.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1013..1933
                     /gene="pstC"
                     /locus_tag="SDA10_000118"
     CDS             1013..1933
                     /gene="pstC"
                     /locus_tag="SDA10_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEG4239053.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            1933..2817
                     /gene="pstA"
                     /locus_tag="SDA10_000119"
     CDS             1933..2817
                     /gene="pstA"
                     /locus_tag="SDA10_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEG4239054.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2832..3638
                     /gene="pstB"
                     /locus_tag="SDA10_000120"
     CDS             2832..3638
                     /gene="pstB"
                     /locus_tag="SDA10_000120"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEG4239055.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3654..4412
                     /locus_tag="SDA10_000121"
     CDS             3654..4412
                     /locus_tag="SDA10_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEG4239056.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4425..5102
                     /gene="phoU"
                     /locus_tag="SDA10_000122"
     CDS             4425..5102
                     /gene="phoU"
                     /locus_tag="SDA10_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEG4239057.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5340..6371
                     /locus_tag="SDA10_000123"
     CDS             5340..6371
                     /locus_tag="SDA10_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEG4239058.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            6368..7135
                     /locus_tag="SDA10_000124"
     CDS             6368..7135
                     /locus_tag="SDA10_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEG4239059.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7235..8803
                     /gene="liaX"
                     /locus_tag="SDA10_000125"
     CDS             7235..8803
                     /gene="liaX"
                     /locus_tag="SDA10_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HEG4239060.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            9100..9420
                     /locus_tag="SDA10_000126"
     CDS             9100..9420
                     /locus_tag="SDA10_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEG4239061.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9420..9770
                     /locus_tag="SDA10_000127"
     CDS             9420..9770
                     /locus_tag="SDA10_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEG4239062.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            9937..10875
                     /locus_tag="SDA10_000128"
     CDS             9937..10875
                     /locus_tag="SDA10_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEG4239063.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10890..11720
                     /locus_tag="SDA10_000129"
     CDS             10890..11720
                     /locus_tag="SDA10_000129"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEG4239064.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11764..12789
                     /locus_tag="SDA10_000130"
     CDS             11764..12789
                     /locus_tag="SDA10_000130"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEG4239065.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12804..13742
                     /gene="galU"
                     /locus_tag="SDA10_000131"
     CDS             12804..13742
                     /gene="galU"
                     /locus_tag="SDA10_000131"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEG4239066.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13867..14307
                     /locus_tag="SDA10_000132"
     CDS             13867..14307
                     /locus_tag="SDA10_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEG4239067.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14310..14912
                     /locus_tag="SDA10_000133"
     CDS             14310..14912
                     /locus_tag="SDA10_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEG4239068.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(14971..16077)
                     /locus_tag="SDA10_000134"
     CDS             complement(14971..16077)
                     /locus_tag="SDA10_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEG4239069.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16287..17288
                     /gene="ccpA"
                     /locus_tag="SDA10_000135"
     CDS             16287..17288
                     /gene="ccpA"
                     /locus_tag="SDA10_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEG4239070.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17625..19970)
                     /locus_tag="SDA10_000136"
     CDS             complement(17625..19970)
                     /locus_tag="SDA10_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEG4239071.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20249..21151
                     /locus_tag="SDA10_000137"
     CDS             20249..21151
                     /locus_tag="SDA10_000137"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEG4239072.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            21364..23139
                     /locus_tag="SDA10_000138"
     CDS             21364..23139
                     /locus_tag="SDA10_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HEG4239073.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(23205..24791)
                     /locus_tag="SDA10_000139"
     CDS             complement(23205..24791)
                     /locus_tag="SDA10_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEG4239074.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            25042..25266
                     /locus_tag="SDA10_000140"
     CDS             25042..25266
                     /locus_tag="SDA10_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEG4239075.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            25408..27159
                     /locus_tag="SDA10_000141"
     CDS             25408..27159
                     /locus_tag="SDA10_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4239076.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            27159..28943
                     /locus_tag="SDA10_000142"
     CDS             27159..28943
                     /locus_tag="SDA10_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4239077.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            29269..30120
                     /locus_tag="SDA10_000143"
     CDS             29269..30120
                     /locus_tag="SDA10_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG4239078.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            30174..31925
                     /locus_tag="SDA10_000144"
     CDS             30174..31925
                     /locus_tag="SDA10_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4239079.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            31929..32099
                     /locus_tag="SDA10_000145"
     CDS             31929..32099
                     /locus_tag="SDA10_000145"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4239080.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(32139..33041)
                     /locus_tag="SDA10_000146"
     CDS             complement(32139..33041)
                     /locus_tag="SDA10_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG4239081.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            33272..34675
                     /locus_tag="SDA10_000147"
     CDS             33272..34675
                     /locus_tag="SDA10_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG4239082.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            34675..36108
                     /locus_tag="SDA10_000148"
     CDS             34675..36108
                     /locus_tag="SDA10_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG4239083.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            36253..37179
                     /gene="trxB"
                     /locus_tag="SDA10_000149"
     CDS             36253..37179
                     /gene="trxB"
                     /locus_tag="SDA10_000149"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEG4239084.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            37393..39120
                     /locus_tag="SDA10_000150"
     CDS             37393..39120
                     /locus_tag="SDA10_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEG4239085.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            39135..39485
                     /locus_tag="SDA10_000151"
     CDS             39135..39485
                     /locus_tag="SDA10_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEG4239086.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(39577..40437)
                     /locus_tag="SDA10_000152"
     CDS             complement(39577..40437)
                     /locus_tag="SDA10_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEG4239087.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            40529..40957
                     /locus_tag="SDA10_000153"
     CDS             40529..40957
                     /locus_tag="SDA10_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG4239088.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            41119..41295
                     /locus_tag="SDA10_000154"
     CDS             41119..41295
                     /locus_tag="SDA10_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEG4239089.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            41322..41768
                     /locus_tag="SDA10_000155"
     CDS             41322..41768
                     /locus_tag="SDA10_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEG4239090.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(41922..43301)
                     /gene="brnQ"
                     /locus_tag="SDA10_000156"
     CDS             complement(41922..43301)
                     /gene="brnQ"
                     /locus_tag="SDA10_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEG4239091.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            44007..44978
                     /locus_tag="SDA10_000157"
     CDS             44007..44978
                     /locus_tag="SDA10_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEG4239092.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            45002..47194
                     /locus_tag="SDA10_000158"
     CDS             45002..47194
                     /locus_tag="SDA10_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEG4239093.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            47211..47687
                     /gene="ybeY"
                     /locus_tag="SDA10_000159"
     CDS             47211..47687
                     /gene="ybeY"
                     /locus_tag="SDA10_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEG4239094.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            47671..48066
                     /locus_tag="SDA10_000160"
     CDS             47671..48066
                     /locus_tag="SDA10_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEG4239095.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            48081..48980
                     /gene="era"
                     /locus_tag="SDA10_000161"
     CDS             48081..48980
                     /gene="era"
                     /locus_tag="SDA10_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEG4239096.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            49123..49935
                     /gene="recO"
                     /locus_tag="SDA10_000162"
     CDS             49123..49935
                     /gene="recO"
                     /locus_tag="SDA10_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEG4239097.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            50319..51236
                     /gene="glyQ"
                     /locus_tag="SDA10_000163"
     CDS             50319..51236
                     /gene="glyQ"
                     /locus_tag="SDA10_000163"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEG4239098.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            51238..53313
                     /locus_tag="SDA10_000164"
     CDS             51238..53313
                     /locus_tag="SDA10_000164"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEG4239099.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        17335 a         9460 c        11367 g        15189 t
ORIGIN      
        1 tgataaaata aacatacttt aggattaaag gagatttcta atgaaaaaac ttaaattatt
       61 cttaccaata ataggattag ctattctttt atcaggctgt tcaaagtgga ttgatcgagg
      121 agaatccatt acggctgttg gctcttctgc tttacagcct ttagtagaaa cagttgctga
      181 aacttaccag acagaaaatc ctggaaaatt catcaatgta caagggggcg gaagcggaac
      241 tggtttgtct caagtacagt caggagcagt cgacatcgga aactctgatt tgtttgcgga
      301 agaaaaatcg gggatcgatg cctcagcatt agtcgatcat cgagtagcag ttgttgggat
      361 cactcctatt gtgaacaagg atattggcgt aaaagacatc agtatggaaa acctgaaaaa
      421 actatttaca ggcgagatca aaaactggag tgaagtcggt ggaaaagatc agccagtagt
      481 tatattgaac cgcgcttcag gaagcggaac ccgtagcacg tttgaaaaat gggtattgga
      541 cggagaaaca gctgttcagg cacaagaaca agattcaagc ggaatggtgc gccagatcgt
      601 tgccgacaca cccggtgcaa tcagctatgt tgctttttct tatgtttccg atgatgtaga
      661 tacgttgaat gttaacggtg tagaaccgac agatgaaaat gtgacaacga acaaatggac
      721 gatttgggca tatgagcata tgtacacaag agatcatcca aaaaaactga caaaagaatt
      781 tttagactat atcttatctg atgaagtaca agataacatc gttggagaac taggttatat
      841 ccctgtttct catatgaaag tcgaacgtga ttggcaagga aatatcgtta aagagtaatc
      901 tttacacaaa atttacaaac ttagctaaac ttttacatgt tattaaaacc aaattgatgt
      961 tcaatttgta cactgagtga gtatgttaaa cctgattaag ggggagtcat ctgtggaaga
     1021 tgtgaaaaaa gtattattaa caaaatcaaa acgttcaaaa atggaacagc gtggaaaatt
     1081 catcagtttt ttatgtatcg cgctgatcgt gttggttgta ttatctattt tttattttgt
     1141 agctagtaaa ggattggcca ccttttttac tgataagatc aatgtatttg attttctgtt
     1201 tggtacagat tggaatccaa gccaaatcgg agcaaatggg aaaccaatgg taggggcatt
     1261 gcctatgatc acaggttcgt ttattgtgac ctttttatct gctatcgttg ccacgccatt
     1321 tgccattggt gcagcagtat tcatgacgga gatctctcct aaaaacggaa cgaagatctt
     1381 acagccggtc atcgagctgc ttgtaggtat tccttctgtc gtttatggtt ttatcggttt
     1441 atcagttatc gtgccttttg tcagaagtat ttttggcggg actgggtttg gtatcttagc
     1501 tggtacattt gtgttgttcg ttatgatctt gccgactgtc acaacgatga ctgttgatgc
     1561 attaaaggca gtgccacggc actatcggga agcttcatta gcgcttggtg ccacaagatg
     1621 gcaaacaatc tacaaagtcg tgctacgagc agccgtgccc gggattttaa cagcagtcgt
     1681 ttttggtatg gctcgtgcgt ttggtgaagc cttagctatc caaatggtca tcggtaatgc
     1741 agcattgatg ccaacaggtc ttactactcc tgcttctaca ttgacgtcca tcttaacaat
     1801 gggaatcgga aatacaatca tgggaacagt tgaaaataat gtgttatggt cattagcgct
     1861 gatcttgcta ttgatgtcat tattctttaa tatcgtgatc cgtatgattg ggaagaaagg
     1921 agagctgaaa taatgaacgc aaaacgctca gataaaattg ctacaggtgt tttatatata
     1981 gtttcgggga tcatcgtatt gattttagca gctttactac tttatatctt agttcgggga
     2041 ataccgcaca tttcatggtc cttcttgacc gaaccttcca aagcctacca agaaggcggt
     2101 ggaatcggga tccagctatt taactcgatt tatttgcttt tgatcacgat gatcatcagt
     2161 tttcctattt ctttaggtgc gggtatctat ctttcagaat atgctggaaa aaactggcta
     2221 acagatgtga ttcgcacgtc gattgaaatc ttgagctcgt tgccttctgt cgttgtcggt
     2281 ttattcggtt tcttggtttt cgttattcaa ttccagtatg gtttctcgat tatttctggt
     2341 gcattggctt tgactgtatt caacttgcct cttttgacta gaacagttga ggagtcttta
     2401 caagcggtcc attacacaca acgagaagca ggattggcct taggtttgtc acgctgggaa
     2461 acagtgataa aagtggtggt tccagaagcg acaccaggga ttttgactgg tgtgatccta
     2521 agttctggaa gaatttttgg tgaagcagct gcattgatct atacggctgg acaaagtgca
     2581 cctgcgttag attttacgaa ctggaatcca ttaagtgttt caagcccgat cagcattttc
     2641 cgtcaagctg aaacattagc tgtccatatt tggaagatca atacagaagg cacgatgcct
     2701 gatggtgttt ctgtttcggc tggcgcttct gccgtgttga ttattgctgt tttactgttc
     2761 aacttcggcg ctcgtgcgat tggcaaacga ttataccgaa aaatgacttc agcctaagga
     2821 gaagaagcaa aatgaaaaaa tataactgga atgaaacaaa tgtcatcacc atgccaaaag
     2881 agaaagaagt agcactatat acggatgatc tacatgtctg gtatggcgac aatgaagcaa
     2941 tcaaaggagt ggatctggaa tttgaaaaaa ataagatcac tgcactgatt ggtccttccg
     3001 gctgcggaaa atctacgtat cttcgttcgt tgaatcgaat gaatgatggg atcgctaata
     3061 cgaaaatcac tgggaaaatc atgtatcaag gcgtagatat taatgcgcct caagtggatg
     3121 tttatgagat gcgcaaacgg attggcatgg tttttcaacg tcctaatccg ttcagcaagt
     3181 cgatttatga caatattaca tttgctttga aacagcacgg cgaaaaaaac aaaaagaaat
     3241 tagatgaaat cgttgaaaca agcttgaaac aagctgcctt gtgggaccaa gtaaaagaca
     3301 gcttggacaa aagtgccttg gctttatctg gcgggcaaca gcaacgttta tgtattgctc
     3361 gtgcgatcgc gatgaaacct gatatcttgc tgctagacga gccggcaagt gctttggacc
     3421 ctatttcgac aggaacggtt gaagaaacac ttgtaaactt gaaagaagac tatacgatca
     3481 tcatcgtgac gcataacatg caacaagcag ctcgtatcag cgactatact gcctttttct
     3541 atttaggaaa agtaatggaa tatgacaaaa ctaacaagat atttaccaga ccaaaaattc
     3601 aggcaaccga agattatgtg tccggtcatt ttggttaaga cggaggcctc aacatgacaa
     3661 aagagattat ttcatctaaa gatcttcatc tgtattatgg aaagaaagaa gcactaaaag
     3721 ggatcgatct aagcttcaat caaggggaaa tcactgcgat gatcggtcct tctggttgcg
     3781 gaaaatccac ttatctgcgt tctttgaacc ggatgaatga cctgattccg aatgtaacga
     3841 tcaccggaag tgttttatat aaagacaaag acatctatgg tccaaaaaca gatgttgttg
     3901 aactaagaaa agaaatcggc atggtattcc agcagccaaa cccatttcct ttttctatct
     3961 atgaaaatgt catctatgga ttgaaattaa aaggggaaaa agataaaaaa gtccttgatc
     4021 aagtagttga agaaagcctg aaggcagcgt ctgtttggga tgatgtgaaa gacaaattgc
     4081 ataaaagtgc tttatcttta tccggtgggc agcagcagcg tgtatgtatc gcacgtgttt
     4141 tggcagttaa tccggaaatc atcttattgg atgaaccaac aagtgcgctt gatcctgttt
     4201 ctactgggaa aatcgaaagt atgctgttag aattaagaga gcggtatacg atgatcatcg
     4261 tgacccataa tatgtctcaa gcttcacgta tttcagataa gacggcattc tttttagatg
     4321 gtcacttgat cgaatacaat gatacgaaga agatattcat gaatccagaa aaacaagaga
     4381 cagaagatta tatttcaggt cgatttggat aaggaggaaa cggaatgcta cgtacacaat
     4441 ttgaagaaga attgctgaat ctccataatc agttctacga aatggggatg atggtgagca
     4501 gcgccgtgca caagtcagtt cgtgcataca tcaaacacga taaaatctta gcccaagaag
     4561 ttatcgaaaa cgatataaat atcaataaca tggaaacacg cttggagaag aaaagttttg
     4621 aaatgatcgc attgcaacag ccagttacaa ctgatttgcg tatgatcatt acggtcatga
     4681 aagctagctc ggatttagaa cgtatggccg atcatgcggt gtctgttgct aaatccacga
     4741 ttcgcgtaaa agggcagacg agaataccgg aaatcgaaaa agagatctcc gatatgtccg
     4801 attatgtgaa aaaaatggtg gacaacgtcc tagttgctta tgtcaaaaca gacgaaaaag
     4861 acgcacgtac gatcgccaat atggatcaaa gagtcaatga ctatttcaat cgaatctata
     4921 atgcaacaat caaaaatatg caggaaaatc ctgagacagt catcagtgga acagactact
     4981 tgaatgttgc cagttatcta gaacggatcg gagactatgt gacaaacatc tgtgaatgga
     5041 tcgtctactt ggctacaggg aaaatcactg agttaaatac gaaccacaat gagattttct
     5101 aaactgaagc tgtgattatg ccgtttgttt aggaaaagag gttgtgaaat cagcggttgt
     5161 gaaaaagctg ttctgcatca agtaataaga acagtcaaac aaaaaatagc tcctcatatt
     5221 tttcgtttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgggaa
     5281 agggtttgtg acaaaacatg tcacaaaccc ttttcatcat ttcatagaaa ggataaagaa
     5341 tgcaaaaaaa aattagcatc atcactatat tttttatttg tgtgcttact tcatgggtta
     5401 ttcagcatca agccgcttta tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa
     5461 aagaggatca tcaattgatt acaggaaaac tgatgaacac ggatcaaaaa ggagaatcga
     5521 tttatttatc ggttccttat caaaaaaatc aaatagaaac agcaaatata aaagcgagac
     5581 aaatgtatct agtccaaatg catggagatg aatggcagct agtagcaaaa aaaaatgatg
     5641 gctggctttt cttcttcaca agtatcttca ttggcgccat gttgcttatt ggtggaaaac
     5701 agggtctatt tgctttagga agtctcatca tcaatatcgt tttactttta cttttgttgc
     5761 tcatctttac taaaacagaa gggatttctc tattaaatat atctatcctg ttcgtcgtta
     5821 tagggatgat tattagcata ttggctttag atggatggaa tagaagcagt ataatcaaaa
     5881 tttcagctgc agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc
     5941 attggaaaga ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt
     6001 ctgtttttct ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca
     6061 cagtcattgc gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt
     6121 tatggctttc aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt
     6181 tgttcgtcta tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt
     6241 cgttcaaaga aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca
     6301 gcttaggaat agtattatcg atccctatct cggtcctctt ttttctgact gtaaggaggc
     6361 tgaaacgatg aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat
     6421 tggcggtacg caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt
     6481 cctgttgtta ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt
     6541 tttattgatt gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc
     6601 agcttttgtg agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga
     6661 ttttttagca atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa
     6721 gactttagcg attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg
     6781 tgctgtttta gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa
     6841 tccgcaatta tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt
     6901 ggcgtcaaca gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct
     6961 ctggtttatt gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct
     7021 agaatatacg atggctattc ttaccacatt gtccgcacta cttgttcttc ctttaacctg
     7081 cgtgactaca gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt
     7141 acaacttaag tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag
     7201 cttgcaaaca attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga
     7261 tttagtgaaa aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc
     7321 aactgaaaaa gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg ctagtcataa
     7381 agaaaaagat caggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc
     7441 tgagccagtt gatccaaagg aaagagaaag acaagatcaa gaaaacttag aaaagatctt
     7501 agatgaacta gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca
     7561 attcgctgac aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac
     7621 aaaagaagaa ttaggcgagt tgacagaaga agaattagca cagcgtcaga cgttagaagc
     7681 agaaatcaaa gaattagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga
     7741 agcagaatta aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga
     7801 taagtttgaa ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg
     7861 cgaaaaaatg tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac
     7921 tgtttcagaa acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg
     7981 gattgcgaca acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct
     8041 tgatatcaag gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa
     8101 agttgaagct aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc
     8161 tgaacggagc cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg
     8221 tgtgcgtgca gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa
     8281 acttctaaat ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc
     8341 tacgaatggt aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt
     8401 aaacggcaat atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa
     8461 tggtacagtg acatttggtg cgacagcaga aaacctttct gtgtctttgg taaatggtga
     8521 tgtacgtctt acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg
     8581 aaatgtcaaa gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct
     8641 tggaagtatc aacagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac
     8701 aaaccaaatg ttgcaattcc gtcgtgtttc agatggtgaa atcgcgcaag tgcaactttc
     8761 aacgacaaca ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga
     8821 tgttcagcct tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat
     8881 tgtgccttat tgccgatagt ctatctcttg aacaccgttt accaagagat tatgaacctg
     8941 ccaattatcg ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata
     9001 tggtaatttg tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa
     9061 cgattttctt catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca
     9121 aaatctaata aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg
     9181 attgatccga ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt
     9241 ccaattattt tatacatttt attagctttg attatcccat ctgcgcgttc tgcacgaaca
     9301 aactatggac ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa
     9361 caaacaaaaa aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa
     9421 tgtcgtactt tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt
     9481 ttcctgcgat ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt
     9541 ctgtgttgaa tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag
     9601 tcacattggg attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt
     9661 ttgtgggagc agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct
     9721 tgatgtcgct tgtcaatgta attgtcagcg aacataacag tagaaaataa aagaaagctg
     9781 tgccgaacta tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg
     9841 gtaattaaat aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat
     9901 gatagagaac actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc
     9961 caattagtga aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt
    10021 gtcatcgtaa ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat
    10081 tattcccata atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg
    10141 atcccagaag agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt
    10201 attatttcaa gaggattacc agctccagaa gaaatgatcc aggcagcaaa tgaaaatggg
    10261 atagctgtac tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat
    10321 ttagatggtc gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga
    10381 ttaggtgtac tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg
    10441 atcaaacgcg gccatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa
    10501 ttaacggtga tcggagaacc gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc
    10561 gggatcattg atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc
    10621 cagttggctg tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct
    10681 gaagacacga ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa
    10741 acaggacgaa acgttgcaat catcattgaa gtagccgcga tgaatttccg cgcacgtaca
    10801 atgggctttg acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac
    10861 tcaggcaacg actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg
    10921 agcttgtagg gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg
    10981 ctatgtggct tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag
    11041 attttatgtt gtggggattg cctatctcca ttattggcgc tcgcttatat tatgttttat
    11101 ttgatcttcc ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct
    11161 tagctattta tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc
    11221 attttatttc tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg
    11281 caatcggtcg ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa
    11341 gcttcttaga aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga
    11401 tctatcgtca accaacattt ctttatgaat cactttggag tttgattgga tttattttgc
    11461 ttttgttttt acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc
    11521 taatgtggta tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact
    11581 ttttaggtgt gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga
    11641 ttctatttat ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa
    11701 aaaatcaaca aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt
    11761 aaaatgaatt atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa
    11821 gtcctcgccg aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa
    11881 gaaatcaaca cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc
    11941 atccaagcat tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc
    12001 attccaacga aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag
    12061 ccagtgatca tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac
    12121 atcttgttag aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca
    12181 agccatgcag aagaagtagc aaaacatgac atcactacga tcacagctgc aagtacagat
    12241 gcaaaagcag caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc
    12301 aatcctgatg tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt
    12361 gctggcgcta ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc
    12421 ggattagctg agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc
    12481 ggattaagcg gaataggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg
    12541 cgcgcaggca acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga
    12601 atggtcgttg aaggtgtgaa tactacccaa gcagcgatgg aactagccca aatgatggaa
    12661 atcgaaatgc ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa
    12721 acagccgcaa aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt ctcattaaca
    12781 attttttgaa ggagcgaatg tatatgaaag ttaaaaaagc agttattcca gcagcaggat
    12841 taggaacaag atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg
    12901 ataaaccaac aatccagttc atcgttgaag aagcgttgaa atcaggtatc gaagacatct
    12961 tgatcgttac aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat
    13021 tagaaacaaa cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga
    13081 cagatgtcaa cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc
    13141 ttcaagcaag agcctttatt ggaaatgaac catttgtcgt tatgctaggc gatgatataa
    13201 tggaagacga agtaccattg acacagcaat taatcgatga ttacgaacgt actcatgctt
    13261 ctacgatcgc cgttatgcaa gttcctcatg atgaaacttc aaaatacgga attatcaatc
    13321 caggagaagt attggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc
    13381 caagcaaagc accaagtgat ttagctatta ttggacgtta cttattgact ccagaaattt
    13441 ttgatgtatt gtccgagcaa caaccaggag ccggaaatga aatccaacta actgacgcaa
    13501 tcgatacatt gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttacg
    13561 atgtcggtga caaatttggt tttatgaaaa caagtattga atatggtcta acacatccag
    13621 aagtaggtgc acctttacgt gagtacatcc tgtctttagg taaaacatta gaagcagaag
    13681 gaagcaagat cgatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat
    13741 aacccttttt tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt
    13801 ttatttgttt aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt
    13861 ttgatcatgt caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt
    13921 gtcgttttta tcgttcgtgt gttgttgcaa gtttctaaaa ctttagaaaa agtagatcag
    13981 actgtagcag aagcaaatac gacgatcgaa atcgtcacaa aagacgtaga tcttttgtct
    14041 agacaagtag aaggcttact ggtcaaaagt aatgaactgc tcttggatat caatggaaaa
    14101 gtcgcaacga tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcagaa
    14161 cttaatcatt ctagtaaaaa catcgcgacg aaagtcggcg gaatcggtaa aacgacagct
    14221 aaagcgacgg ttgccggaaa agtcggtggg acagcgatga aattttttaa aaacaaaaaa
    14281 catactgaaa cgacaggagg aaaataaaaa tggctaaaaa aggtggattt ttattaggtg
    14341 cagtagtcgg aggcacagcc gcagcaatcg ccgcgttatt gttagcacca aaatctggta
    14401 aagaacttcg ggatgaatta gctgcccaag cggatgattt gatggatatg gcttcagatt
    14461 acacagatat ggcgaaagaa aaatcgactg agttaacagg tattgcaaaa gaaaaagcgc
    14521 aagatctttc tcaacaagca ggtgatctag caggtagtgt caaacaaaaa gtaagtgaat
    14581 cagcagatga agcaggaagt gaatctgacg atatcttgtt aaatcttaaa gaacaatcag
    14641 ctgacttatc taatcgtttc aaaaaaacgg cagaagatat gaaaaaacct gcagatgaat
    14701 taggaagtat tgctaaagat acttcagaag atatcatgat tgatgtaaaa gacgctgctg
    14761 gtaaagcagg agatactgtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgact
    14821 caaaagaaat gatgaaaatg gcaaaagaag atgcgaaaga agctaaaaaa gaaataaaag
    14881 atacaatgac agattcatct acaaatcact aaaatcacaa atttatagaa taaaaaggtg
    14941 ggagcggact agctctcacc ttttttactc ttatttttct aaaacaagta attcttttgg
    15001 tgtagtcgtg aatggcacac agccatcgct tgtcacatat acacagtctt cgatacgtac
    15061 ccctactttt tcaggaatat agattcctgg ttcaagagag aagcacatcc cttcttcgat
    15121 tactaagtca tttccttcta caagggaagg gaattcgtgg acggttgttc cgatcccgtg
    15181 acctaaacgg tgattgaaat attcgccgta gccatagctg ttgatcacgt ttcgtgcgat
    15241 ttgatcaagt tcacctgctg tcacacctgg acggacggct tcttgtgcgg caagctgtgc
    15301 ttcaagagtg atgttgtaga ttttttcttg gaagtcggtt ggtttttgat aagcaattgt
    15361 acgagtagca tcactgcaat agccattcca aacaacgcca agatcgaata acacaagttc
    15421 attcggtgaa atcttcgtat ttcctggtgt accatgaggg cttgcagcat ttgctcctgc
    15481 aaggactaat gtgtcgaaag acatttgtga aacaccttgt tttttcaatt ggtattcaat
    15541 ttctgcaatg atcgcttgtt cagtcacgcc ttctttcact gctttaaagc caatctcaaa
    15601 tgcaacatcg gcccatttac cagcttcgat cagtttgtct ttttcctcta gagttttgat
    15661 cagttgtaga cgttggatga tggaagttag atcagtgtaa gaatccgttc ccgtcaagat
    15721 atcagagata gcatcgaagc gctcgacagt caaggcgttc ttttcaatcc ctacattgcc
    15781 aggctgacca tagcgagcac gaagttcttt tccgatgatc tcccaagggt tttcgctgtc
    15841 taaatagcca cggacatcat acgcccaact actgttttga gcatcttcaa cttccaaagc
    15901 aggagtgaaa aggaaaggat cttgttcaag agggataaaa agagctagta cacgctcatg
    15961 aggatcactt tcgtatccgg aaaagtaagc gatgtggcca gggtcgctga tataagccaa
    16021 ttcgacttgg tgttgtgcca tccattgacg tagttcattg atttttacgt tattcatttg
    16081 gcaacctact ttctaaatta attttgtatt caataattgt accactgatt tattaaaaga
    16141 ccaacgttct aaaaactgaa aaaagtaaaa attttcatga aaacagagat aaaacggttg
    16201 caattcttga gattatttga tattctattt tagaaatgta agacgtattt tcagaaaaca
    16261 tataaaatat gaataggaga acacaaatgg aaaagcaaac aatcactatc tatgatgtag
    16321 cgagagaggc aaatgtttcg atggctactg tttcacgtgt cgtaaatggc aatccaaacg
    16381 ttaaaccagc aacaagaaaa aaagtcttgg aagttatcga tcgcttggat tatcgtccaa
    16441 acgcagttgc gcgtggcttg gcaagtaaga aaacaacaac agttggtgta atcatccctg
    16501 atgtcagcaa tatgttcttt gcttctttag cacgtggtat cgatgatgtg gcaacaatgt
    16561 acaaatacaa tattatctta gcaaattcag atggcaacga ccaaaaagaa gtcaatgtat
    16621 tgaataactt actggctaag caagtggacg gcgtgatctt catgggtcac catatcacag
    16681 acgaaatccg tggagaattt tcacgttcaa aaactcctgt tgtattagct ggctctattg
    16741 atccagatga acaagtaggt agcgtaaaca ttgattatgt atccgcaaca aaagatgcag
    16801 taaacaaatt ggcaaaaagc ggcaataaaa aaatcgcttt tgtcagtggt gcgttgatcg
    16861 atcccatcaa tggacaaaat cgtctgaaag gctataaaga agcattaagc gaaaataacc
    16921 taccatatag cgaaggattg atctttgaag cacaatacaa cttcaaagat ggtctatctc
    16981 tagtcgatcg gatccataat agcggtgcaa cagctgtcta tgtttcagat gatgaattag
    17041 ccatcggtat tttggatggt ttattagacc gtggcgtgaa agtaccggaa gaattcgaaa
    17101 tcatcacaag taacaattca ctactgacag aagtagctcg tcctcgttta acaagtatca
    17161 cacaaccatt atacgattta ggggcggtat ctatgcgcct attgacgaaa atgatgaata
    17221 aagaagaagt cgaagaaaaa acgattatct tgccttatgg aatcgaagaa aaaggatcta
    17281 caaaataata aaaggacaac cattcagagg ttgtgtgcct gccgtttaac tctgcgttac
    17341 aaggagcaat ttcaaaaaac agcatctcat gtttgatgaa tattgtgact cgtgattcgg
    17401 agttggcagg agaacaccgt ttatttggga aaaggggtta tgacaaaatt ttgttataat
    17461 cctttttttg tgtcttaaaa aagagagaaa gtggttgaaa ttacatttat cgtatataaa
    17521 acaatccgtg tatagatgct gcattttgtt caccaccatc aaaaaaataa gggactgtga
    17581 caatactatt gtcacaatcc cttattactg tgtattctat taaatcaatc atctttctca
    17641 tctgattctt tagttgcttg attcgctctt gcataagctg aagcggtgtt ccagtaatct
    17701 gtatagtttt catcggtacc gccaacgcca aatttaaagg cgcttttctt cggtccattt
    17761 ttcgcccata gcgattcaac tgtttttcct ggtgtttgga tggatttttt attgacagta
    17821 acttttccag gtgtttgacc agtaaatcca gatacttttt tcttcatcac atcagatgat
    17881 aattcgaatt tctcatcagt gccaaaaatg tcaggttttg cttggtaagc ttgattgatc
    17941 agattggcaa gataattagc cgaacgtatc cttgctttgc tgtccattcc agtattgtca
    18001 tcgtgccctg cccagctact gatggtaata gacggagtag agacaattag ccatgaatct
    18061 cggtattcat tggtagaacc agtttttccg acccaatctg cttttcctag atttccgttt
    18121 aagcttgaga tggcatcttt gaatggtgta gtgatttttg cattgatcac actacgcatc
    18181 atgtcattca tgatcgatgc cgttgcttcc gaatagatcc ggacagagtt gctttcgtgt
    18241 ttataaatta catttcctgc gttatctgtg atggaatcga tgatatagcc ttcttcatag
    18301 acgccgccgt ttgctaaagc ctgaaatccg ttggtctgtt gaagcgtcgt gacatctact
    18361 gtacctaaag gagcagattc aacgccccaa ttgtcatttg ccgggtagtt catctttgcc
    18421 aagtagtgct cgtaagcata ttgtttcgat ccaccggaat tcaacacatc ttgatataag
    18481 tgataagccg ggatattgtt ggaccattct agtgactcac ggacggtttg gaaggtgttc
    18541 gatccttcgt tagtagcatt gacgattttc tcgccagcat tttctccttc ttgccaagtt
    18601 gttgcataat ctgatacacg ggattctgat ccgataagcc ccatgtcgat tgcaggtcca
    18661 taaacaagaa ctggtttgat tgatgagcca gcttgacgtt ctgtatcgaa ggcatggtta
    18721 ttttgattct gactgtaatt ccgtccgcca acgaatccgt agatgcggcc agttctgtta
    18781 tccatcaata catttccggt ttctacttga ctgccagcac catcatctaa catgtatcca
    18841 taattttcaa cgccattttg caatgcagca tagacatctt tatcgattgt cgaatggatt
    18901 gtatatcctt tattttggat cgtttgctgt gccttttgat aataagcgtc actgaccgat
    18961 gaatcagaca tatcagcgtt gtctttttca gccaattgtt gtgcaatgat tcttgttgct
    19021 tcattcatca cagtataata caagaattct ctgtcagtct gttctgcgat ttgttgtggt
    19081 agaaagtctt ttgtaaggtc ataggctttt gcttcttcgt attgttcttt cgtaatttgg
    19141 ccttcacgat acatactgaa taaaacaaaa tcttttctgg caaggccggc agaaagatct
    19201 tcttttagcg cgcctgtgtt cgtgtatgga ctatacgtga tcgggctttg gggtaatcca
    19261 gcgatataag cagcttgcgg cagcgtcaca tcttttgcat ctacaccaaa gattcctttt
    19321 gcagcttcct ggactccagc aatgttttgt cccttattat tacgaccaaa tggtgagaca
    19381 ttcaaatacg tagcaatgat ctcatctttt gaaaagtatt tttctacttg tgcagaaaga
    19441 atgatctcgt ttgctttacg tttgaatgtt gtttcatctg taaggatctg ctgtttgact
    19501 aattgctgtg tcaaagttga tccaccagaa gagccaatac cagtagcttc agatactaat
    19561 gctcgtaaaa cagctttagg aacgtaacct tggtgtttat cgaagtattc atcctctgtg
    19621 ctgataattg cagttttcaa taaaggtgaa atcttatcag atgaaatagt cgtgcgcatc
    19681 agatctgtct ggatttttga tatctctgta ttatctgcat aaacaatctt tgacgtttct
    19741 gtaatgtttc ctagttctgt ctgcagtgct tttttagtcg gcaattgtgt gtcttcgacg
    19801 agataggcaa aatagccagc gcctattcca atacctagag ctgcgaacat caaaagaaga
    19861 acagtaataa aaacagtcaa tgattgaaaa actctaaaaa taatcttagg aacaaaccat
    19921 ttttcttttt ttcttctatt ctgttttttt cgagtatttt tgcgtggcaa aagggtcacc
    19981 tcaactaatg gttttatttc gctaattata gcaaataata gaacaaaaaa acagacaagg
    20041 ctgtttttgg caggaatttc ttttttaaaa gagttaaaac gtgagaaaat gatctttatt
    20101 ataaaagagt tcttaagctt ttctttagag aaaatttcag ttactatgga gaacagggca
    20161 aagaaaaatc aatcattttc tttgtatatc atcttaccaa tatataggaa ttgttagtaa
    20221 caattgcctt tcaaggagga atccatccat gttattaggc tcacacgtca gcatgagcgg
    20281 aaagaaaatg ctgttaggag cagcagaaga agcagccagt tatggatctt cgacttttat
    20341 gatctataca ggcgcccctc aaaacactag aagaaaagcc atcgaagaga tgaatattga
    20401 tgccggaaaa gcgtacatgg cagaaaaagg tctaacaaat atcgtcgttc atgcccctta
    20461 tatcattaat ttggggaata cgatcaaacc agaaatgttt ccttttgccg tccaatttct
    20521 gagagaagag attgttcgtg cggaagcttt aggcgctact caaattacac ttcacccagg
    20581 tgctcatgta ggggcaggaa cagaagccgg aattgctaga attattgaag gcttgaatga
    20641 agttttgaca aaagaacaga cggcacaaat cgctttggag acaatggcag gtaaaggtac
    20701 ggaaatcggt cgaacatttg aggaattagc gcaaatcatc gatggagtaa aattgaatga
    20761 aaaattatcc gttacttttg acacctgtca tacgaatgat gccggttata atattaaaga
    20821 agacttttca ggtgtaatgg aagagtttga taagatcatc gggttagatc ggcttaaagt
    20881 actccatatc aatgattcta aaaatccgca aggttcgcat aaagaccggc acgccaatat
    20941 tggttttgga acaatcggat tcgacacgct gaataagatc gtccatgatc caatgtttga
    21001 caatatttcg aaaatcttgg aaactcccta tgtaggcaaa gataaaaaga aagcttacgc
    21061 accatatgga aaagaaatcg ccatgttcaa agcgcaaaca ttcgatccag aagtgtttcc
    21121 tgagttatta gcagaagaag tgatactcta agattttcta tctgacagca gcaaaaaagg
    21181 accaggcaca ttgccttgtc cttttttgtt taagaaacaa aggagtactt tttgcagtct
    21241 ttttcatcat gcatcttctc ccccctcttt ttaaatttag acaaacaagg tatactggta
    21301 gaaaagtaaa aaaacgaaaa gtttcacaag caagtggcaa aacgtgattg gaaggacgaa
    21361 aagatgctga acactttaaa acccttgatc aaccaactaa aaaagaaaca tacatacttt
    21421 attcttggca tagccctatt atttacaatt attcgtttgt atttatatag taaagcaatc
    21481 tatggaacag acatgtcggc aacctatgat gaccagctgc tgattcgcta tgctcaaaat
    21541 attttggacg gtaaatggct aggggagtat acgactacaa cactttccaa aggaatctcc
    21601 tacagcttat ttttagtact tgcacataaa ctgcatcttt cttatgcagt tttgttcggc
    21661 atgttcagta tttttgccag tatattggtt gcactcgcgt ttaaaccgat tgtgaagaac
    21721 aagtatcttc ttttaagtat ctatttttat tttttgtttt ctccgattag ttttacacat
    21781 gaatattcca ctcgaacata tcgtaatgcc ctcgtgattc cggctgttga gatcattatc
    21841 gcttgtttat tagctattta ttaccgcaaa tacggttcta gcagaaaatt gctgccatgg
    21901 tttttagggc tttctgtcgc ttttcctttc ttttggttca ttcgcgaaga ttctatctgg
    21961 cttcttcctt ttactgtagt agctttgggt atcagtttcg tccagatctt gctagggcaa
    22021 gacttttctt tcaaagaagt aaagaagtta aaaaaacgac tctttcttct ctcgaaacgg
    22081 gaatggctga aactttcatt gtttttactt cctatagttg gcttttccat cgtctatcaa
    22141 tggatcgaat ataaaaatga gacaacatac ggtattgcaa ccatcaatga ccgttccagt
    22201 ggagaatttg gacgtttgat gaaacaactg atccgaatag atgatgaaac gaacttgaat
    22261 aaagaaaaca gtgagatatg ggtctcacat gctgcactaa ataaagcatt agccgcatct
    22321 cctacattcc atactgtttc tacccgtatt gacgaattat atacgactca cgcgtggact
    22381 caaggtggaa aagtaaaaga attacccgga gatatcatct tttgggcatt gcgagatgtg
    22441 gtaagcgaat ctggttatta ccaaaacaat gcaaaagaaa ccaatgattt ttggaaaaaa
    22501 gtcaaccaag aattagaaga agcctatcac aagggaacaa tcaaaaagaa aaaagagttc
    22561 tatctgatgg gatcaggaga tggaaagacg atgagtgatc tgccaatcgt attcgatttc
    22621 cttcaatcag gatacgctta tgcatggcga tataaagatt tccatcaagg aggaaattat
    22681 agtttcggaa cgcaagaaca agtcaaacag gctcaagaat tattaaacct tccgctaatg
    22741 gagaattgga tcgatagtaa tgaaccgaga acacctaatt tccaattaac aaaatcagca
    22801 aaagtcgcga atgtcatcat aaaaatttac caagttctgg ctattcccgt gctgattgct
    22861 tcaatagcag ggatattact tatgatacta ggaaccgtat ttgcaaaagc tgatcgtgcc
    22921 tattatagtt cctctttgat tgtggtcacc ggattaatac tgacacaatt ggtttttctt
    22981 ttcggtgtct catggttttg ttcttatgcg ccagaaaaaa aggactattt tttaagtgtc
    23041 tacactggtg caggcgtgcc attaacgcaa ttcatcgtag taacaagcat gctagttttc
    23101 agtaaattaa atctgaaaaa agctttatct tctaaataaa aagacccttg gtagctagat
    23161 ttttaaccta actatcaagg gtctttgtgt ggaaaaatca gtttttattc gatttcttct
    23221 accgtttcct gcaaaagctg ttcatatttt ttgtttcctt tatataaatc accaatcgtc
    23281 catgccaaca ggagaaggac aagaacaatg attacataag cgatgtgatc agccaatgct
    23341 agttgagcgg aagtggcttg ctcgccgaag aaactttcga tctgtcctgg taatcctcgt
    23401 aagttcaagt aagttagact aatcacagag aaccagccaa gaagttttac gatgaatgaa
    23461 tttttaaatc ggctacccat ctccgcttgg ctgttggtca tcatcagtaa aggaatcatc
    23521 gaaaacggca acgcaaaagc caagaacacc tgggattcat tcattaagtt attcaaagcg
    23581 gcatgttctt cgatcgttcc ttttgtactt gtaaacaaaa cacaaatcaa tacaggaatc
    23641 acagaaagca atcgtgtgac cagtcttctt agccaaatcg ggatacgcat atggatgaat
    23701 ccttccatga tgacttgacc ggtcaacgta ccagtaatag tagagttctg gccagaagca
    23761 agaagtgcta cagcaaataa agtggacaag ataccagatt ttgctacagc aatcaaaata
    23821 ccattgctca tagctgatgg atcagataaa gcttcataca gaccaaaaaa ggaaggatct
    23881 tggacagaac cggttttgaa gacagcgaca cccatgatca atagcagtgc attgactaga
    23941 aaagccattg acaactggat attagaatcc caagtagaaa aacgtacagc ttgtgctaca
    24001 tcttcttcgt tttggtgatc gatcttccga gtttgtgaaa ctgctgaatg aagatataag
    24061 ttatgcggca tgactgttgc accaataatc cctaaagctc cagtcagcgg agtttcgccg
    24121 ccgatgaccg gtgagtctga gaatgtatga ctgttaggga taaagccagc aaataaactg
    24181 tgccaatctg ggttagacaa gccaacttga tagacaaaaa cgaataaaat gacgaaaatt
    24241 aagcagacaa ccagtgcttc gatcttacga aacccgactt ttgtcagtag taagagaacg
    24301 aaaacatcta atacagtaat aaagacggca atcaccaatg gaatatcaaa aagtaagtag
    24361 agggcgattg ctgcaccaat gacttctgca atatctgttg ccataatggc taattcagtc
    24421 aagatccata aaatgacacc taatgtctta cttgtgcgtg ctcggattgc ctgtgccaaa
    24481 tccatttggc tcacgattcc taattttgct gccatatact gtaaaagcat agcaatcaga
    24541 ctagacatca aaataactga catcaataaa tattggaaat tttgtcctcc ggtgatcgaa
    24601 gtagaccagt tacccgggtc catataccca actgctacta acgcaccagg tccagagtaa
    24661 gcaagaagtg ttttaaaaaa acttttcccc tttggtacat caactgttcc atttacttca
    24721 gctaaagata aaccatttgt atgttcaata agtcgatgct tatgaaattg tttattggat
    24781 tgattattca agaaaaaacc acctttccta tttatctacg attacattct acacctcaaa
    24841 aagaaaaaat taaagaaaaa gattcggtac gcctaatttt ttttcatggc gcgtcttgct
    24901 agatgacgaa agaaattttg ttctcctcta aaaaaaccac tattttttgc atgaaactaa
    24961 agaatacgcc tttctttttt caataaactt tagtaaaata agtcattgag agcaattcgg
    25021 aaatagaaag gagagattta tatgttatca acaggtattg tcgtacttat tgctattatt
    25081 gctttgctaa tcggcgcagt aggtggcttt ttcctagcgc gtaaatatat gaaagactat
    25141 ttagaaaaaa atcctcccgt taatgaggac atgttgcgat caatgatgat gtcaatgggg
    25201 caaaaaccat ctgagaagaa aattcgccag atgatgcagc aaatgaagaa ccaagggaaa
    25261 aaataggttc tgacccaacg actttttacg ggctgatttt ttcagtccgt tttttatttc
    25321 gtaatttgtt agactattca gattattcag caaaaaagac aagcacagac aatcctaggc
    25381 tcttgataaa gacaaaggag gagtaaaatg tcgattttta gaaaattagg ctggtttttt
    25441 cgtcaggaaa agaaacatta tttaattggg gttttctcgc tattcgcagt ggccttggta
    25501 cagttagttc ctccaaaagt gattgggatc atcattgatg agatcgccga taaaaattta
    25561 tcaataaaga tcattttcgg gtggatcttc ctattgatta tcgctgcttt tttacaatat
    25621 ctttttcgtt atatctggcg gacgaatatt tggggaagtg cagctcgatt ggagaaagaa
    25681 cttcggcagc agttattcaa acattttaca gaaatggaca atctctttta ccaaaaatat
    25741 cggactgggg atttgatggc tcacgcaacg aatgatttga atgcgatcca gaatgtagca
    25801 ggagctggta tcttgacctt tgcagattca gtgattacgg gcggaacaac gatcattgcg
    25861 atgattttgt ttattgattg gcgcttaaca ctgattgcgc tgattcctct gcctttgctg
    25921 gccgtcactt cacgagtgct ggggtcaaaa ctccatgatg cttttcgtga ttcgcaagaa
    25981 gctttttcta gtatcaatga taagacacaa gaaagtatca ctgggatcaa agtcattaaa
    26041 acatttggac aagaaaaaga agatctagcg gattttgaag aaaagattga agatgcaatt
    26101 attaaaaaca aacgagtcaa ttttttagac tccttatttg atccattcat taccttgatt
    26161 atcggtctgt cttatgttgc aacaattatt ttaggtggaa agtatgtctt ggaaggcacg
    26221 atctcgatcg ggcagatggt ctcatttatt agttatatcg gtatgcttgt ttggccgatg
    26281 tttgcgattg gtcgattgtt caacgtattg gaaagaggaa atgcttctta tgatcgcgtc
    26341 aatgaacttt tgcatgaaaa gacgcatatc gtggaacaac tgggtgcagt gcagaccccg
    26401 gcaaaaggcg agctgaatgt agctatttcc tcttttaatt atccgaaaga agaatcacca
    26461 gcattagaac aaatcaagtt tgcgatccaa gaaggagaaa cattaggtat tgttgggaaa
    26521 actggtgcgg gaaaaacgac gatcatcaaa ttactgatgc gtgaatacga tgattatcaa
    26581 gggaaaatcc aatttggcgg acgttcgatc aaagaatatt cattgaatgc tttattagga
    26641 tcaatcggct atgtgcctca ggatcacttc ttgttttcga tgaccattcg agataatatc
    26701 cgtttttcta atccaaaact ttcagaagaa gaagtagagc atgcggcttc tttagcattt
    26761 atcaacaatg aaatcaagga attaccaaaa ggttatgata ccttagttgg agaacgtgga
    26821 gtctctttat ctggcggaca gaaacaaagg atttcgattg ctcgagcatt gatcaaaaat
    26881 cctgaattat taatattaga tgatgcactc tctgcagtag atgccaaaac agaagaagct
    26941 atcttgcata atttaaaaga aataagaaaa gagaaaacaa ctatcattgc agctcaccgt
    27001 ttaagtagcg tgatgcatgc aaaagaaatc attgttgtcg atcaagggaa aatcattgaa
    27061 cgaggaaccc atcatgagct gcttgcacta ggcggctggt atgaacaaat gtgggagaaa
    27121 cagcaattag aagcgaagat agaaggaggc gaggcctgat ggatgaaaat tatcaatcgg
    27181 aatggacaaa atcgattcca cttaaagaac agaaaaagat catcaaacga ctgatgaagt
    27241 tcgccaaacc ttttagaaga acgttcatcg ttgctatctt atttgcgttt gctttgtctg
    27301 tgatcaatgt cctattgcca aggatcatcc aaacttttat ggatgatcat ctggctaaac
    27361 agtcggctac gacacaagtt attttgtttt ttgctggtat gtacttattt ggagtcatag
    27421 taaaaagtat catttggttt ttccaatggt atttgtattc catggcctca ttgaagacct
    27481 atcagtatat tagggtaaaa ttatttgaaa agctgcatac actaggtatg cgttattttg
    27541 atcagacacc ggcaggatca accgtttctc gagtgacgaa tgacacagaa acgctatttg
    27601 aattttggta cgtctttttg atggtattga ctgggatctt tgcagtcgtt tcttcattcc
    27661 ttgccatgtt tcaaatcaat ggagaaattg ccttgtggac tttggtgttt ttgccgattt
    27721 taggaatcgt catctggtat taccagaaat tcagttctcg tatttaccga aatatgcggg
    27781 aaaaattaag tcagctgaac acgaaattga atgaatcgat ttccggcatg cgtattatcc
    27841 agcagtttcg ccaagaagct cgtttagcta aagaatttga acaagtgaac aatgagtatt
    27901 tagacacgcg ttatgcgatg atcaaaacga attcgattct gttaagtccg atcatcaatt
    27961 ttctatatgc attggcgatt gcgttggtgt tgactttgtt tgggatcgat gcgttgcatt
    28021 ctcccgtaga agttggactg atctatgcat ttacgaccta tgtccaggga ttttttaatc
    28081 caatgtctca aatgatggac tttttgagca tcttcacaga tgggatggtt gctggtagtc
    28141 gtatcttgaa gattatggat acggaagaat atgcaccaca acaaaaccct ggagcagatc
    28201 aaatgattac ttcaggtaag attgagtttc gcaatgtaag cttttcttat gatggagagc
    28261 acaatgtatt aaacaatatc tcttttgttg ctaatccggg agagaccgtt gcattagtgg
    28321 gacacaccgg aagcggaaaa agctcgatca tcaacgtatt gatgcggttt tacgaatttt
    28381 atgaaggtca gatattgatt gatgatcatg acattcgaga atatccaatg gaagagttac
    28441 gtaagaaaat ggggctggtc ttgcaagatg ctttcttgtt ttatggggat atcgcaggaa
    28501 atattcggat gatgaatcca gctatcacag acgaacagat acgccatgcg gctgagtttg
    28561 ttcaagcaaa caaattcatc gatgaattac caaaaggata tcactcaaaa gtgatcgaac
    28621 atggtgccag ctattctagt ggacaacgac agctgatcac ttttgcgcga acaatcgtga
    28681 ctgatcctaa aattctagta ttggatgaag caacagcaaa tatcgatact gaaacagaag
    28741 gattgatcca agaaggattg gcaaaaatgc gtcaaggacg gacaacgatt gcgattgccc
    28801 atcggctgtc aacgattcga gatgcagaat tgattttagt tttagacaaa ggtcatatag
    28861 tagaacgcgg gaatcatgaa acacttctag cgcaaaatgg tttgtatgca gatatgtaca
    28921 aactgcaaag cagtggtata taaaagatgt tgtgacagta gaattttgac ctaagcacta
    28981 gagtgaaaat aaaacatagc ttctcatatt tttcatctgt gagagttagt gtcgtagtca
    29041 ccagatttaa aaaaagattc gacagctagt cagaagtgcc taatagccac taagaaagcg
    29101 ttgacaaaag catttgcttt cgataatata aagaaaacga tacaacttgg atagtgattg
    29161 cctgatgcaa gctaaacctg atgagtctcc catggtttca tggacggctt tttgggtttt
    29221 tctttttgtt ttggatacag ccctatacat catggaaagg agcaaaacat gctgattgag
    29281 aaagtactga ataacaacgt cgtaataacc agggatgaaa gaaatcagga aatgatcgtg
    29341 atgggaaaag gtttggcatt caaaaagaaa gtgggtgacc cgatttttga ctcatgcatc
    29401 gacaaaaaat ttcagttgac aaatgacggg ctttcacaac agttccaaga attggcacaa
    29461 gcgattcctc ttgagtactt agaaatttct tgtgaaatca ttgaatatac aaaagaggct
    29521 ctacaagtcc agttgaatga ttctgtgtat attgcattga cggatcattt gtatacggct
    29581 atcgaacgtc acaaaatggg aatatttgta ccaaatgtat tgctgtatga tatcaaacaa
    29641 ctatttccta aagagtatgc catcgggaaa aaaacagtga agaaaatctc ggataaatac
    29701 cacatcaagt tatcagaaga tgaagccgga tttatcgcat tgcatttagt caatgcacaa
    29761 ggcattcaaa gccagcaaga caatacctac ctgtttacta taacaataca ggatattcta
    29821 aatatcgtcc gctattacta taagattaca tttgatgaag attcagtcca tttttttcga
    29881 ttcgtcacac acttacgttt ttttctgaca cgcgtcctag atggagaaaa atcagaagtg
    29941 ggagaagttt ctgaggaatt gtttcaggtt attcaaaaaa aatataagga tacgtttggt
    30001 tgcgttgaga aaatagcaga ctttttagcc gagacgttca actaccagat gtccacggac
    30061 gaaaaaatgt atttaacgat ccatactgcg cgtttagtgc atgagaatcg ttgtaaataa
    30121 aaaaaggagg ataaattaac aagaacgtat gaaataaggg tagtgacatt cagttggcta
    30181 aaccttccac taataaattg gagggaatag aaatgggaaa ataccaagaa ttagcgagaa
    30241 aaatcgtaga aaatgtcggc gggaaggaaa atatcaatgg tttgacacat tgtatcacaa
    30301 ggcttcgttt caaattaaaa aacgaagaaa aagcaaatga tgagatcttg aaaaatatgg
    30361 acggtattgt aacggtaatg cgagcgggag ggcagtatca agttgtcatt gggaatcatg
    30421 tgccagtcgt gtttgaagag gttataaaag caggaaacct tacttttgat gaggctgttt
    30481 caacagaaaa aatgagaccg tttgatatgc ttattgatgg aatatcgggc tgtttccagc
    30541 cattccttgc tatcctagct gctggcggga tgattcgtgg actgacagca ttccttgtct
    30601 tcttaggcgc gtttgaccgc ggttctggca catttgttat gtttgataat atcggtgatt
    30661 ctgtattcca atttatgcct gttattatcg gtttaacagc tgcccgtaaa tttaaagtaa
    30721 atgaattcgt agggatgttg cttggcgcag cattaatgaa tcctagcttg tcgctggaag
    30781 ctttgagtgg tgcagcagaa gcaccactaa cgaccatatt ttctggcact atttttgaag
    30841 cacctattta tcaaacagta ttcggcattc cctggattgc gcgtaactac gcttcttctg
    30901 tcattcccat tatctttatt gtgttgttag ctagccaaat tcagaaacca attaaaaagt
    30961 tagtacctga gatgatcgcg aatttcttcg ttccattctt caccgttctt attacaatgc
    31021 ctttaggttt tttactcgtt ggaccagtat tcacattcgc aactgatatt ttaatggctg
    31081 gatttgaaac acttttagca ctttcgccag ttatttatgg agcaatcgta ggcttcttct
    31141 ggcaaattct ggtgatgttt ggtctccatt gggcaatcgt accaatggga ttgatgcaat
    31201 tttctgtgaa tggttggcaa aatattatga cacctgttgc agtagtgtct tttggacaaa
    31261 ctgcagcact taccgcactc tactttaagc ttagaaatcc aaaagacaaa gcaattgcaa
    31321 taccagccat tgtttctggt attgtgggga ttacagagcc agctatttat gggtttacat
    31381 taccacgtaa aaaaatattt atttttacct gtgtcggtgg tgcgttaggg ggcgcgtata
    31441 gtgggttaat gaatttaact tcttggaatc aaggtggact aggcatcttt acaattccca
    31501 actatattag accagatggt gatttaacag atgtgattaa tgtattgatt gggatcgcca
    31561 ttgcgatggt ggtatccttt acgctgactt ttttcttttg gaaggatgaa gcaggtgaaa
    31621 cagatgatat acagaaaaaa tcaggtaaag aaattgtaaa aacaccgata caaggacaaa
    31681 tcgctccact aaatgcagca aaggatgcag cttttgcaca aggaacacta ggtcgaggaa
    31741 tcttgatcta ccctgaaaaa ggagaagtac gtgccccttt tgacggaaca gtcatgaccc
    31801 tgttcccgac gaaacaggca attggaatgg tttctgaaac agggctggaa ttgctgattc
    31861 atgtcggatt ggatactgtg cagttggaag ggaaatattt tgaaagctta gtgcagcaag
    31921 gataaagagt gaaacaaggc gatctgttgc tacgcttcga caaaaagaaa atcgaaaagg
    31981 aaggatacag tttagaaaca cctgtgatcg tcaccaatta cacggattat ttggatattt
    32041 tggaaaacca aggggagacg gtcgggccag acgatgcact tatcaccgca ctgacctgaa
    32101 cggttaggtg aagaatggac taggcttaac tattcttttt attccacttc tttattttca
    32161 aaattgaaag gctctttcat gatggctgag ctgatagggc gtttatcgaa tttttgaccg
    32221 acagaaatta gatggactaa attctgttga taattttctg caaaaacaca actatataac
    32281 acgtctagaa gataacaaat tgatgtattg atcgtaaaat taccaatttt tgaatacagt
    32341 cgctcgcgtg tcgtaagtcg caaagcggct gtactcattg cagatagcgt tgaatcaccg
    32401 atactggtca aagctaaaat tggaacatga ttttgtttca atatttctgc ggtacgaagg
    32461 atcacattgt tttcacccgt ataagaaatc agaatcccgc acatatcact cgtgcaattg
    32521 tacgcttcat agaatgattc accaaaagtg gtgacaatcg tgacatccat tttgatgcgc
    32581 ttcattttca gcatgaaatc ttgagaaatc aaagtattcg agttactggc gaaaatcacg
    32641 attttttttg cttgaagcat tagttgtttg gctttttgca gttggtcatg atgaatgaga
    32701 gaaagtgtat catgaatcgt catctgttct aaagaagcca ttttatttgc gatcgtcata
    32761 ataccgtctg tggtggaaaa aggaagattc gcatctacgt cttgaaaatt cccttcaaga
    32821 taggctgttt cagctaaaaa agcattacga aaactgaccc agccatcaaa acccattttt
    32881 tttgctacac ggatgagtgt agaggggtgg acataacatc gctcagcaat cgcttggatg
    32941 gtcatttcgc caagtgctgg agaatcgctt aagagaaact ggatcacttc attttctgaa
    33001 ggagaaaagg agatttcttt gattttttca cgtaataaca tgtagatacc ttcttcttat
    33061 ttttttacgt aaattataac ttttttgaaa atgatttgta aacggattta tactatataa
    33121 cttaaaatag acattttttg acaagatatt tacaaaaatc cactagaaaa cagtaaattt
    33181 tgtaaatgtt actggaaaga aacagatttc tattgtttgt tttgtttaca aaaaagataa
    33241 tgaaagtgta aaaaagtaaa ggagtgagaa catggcaaaa ggaatcaaaa tcgctacgat
    33301 tggtggaggc agcagttaca cccccgagtt gatggaagga tttatcaaaa gatatgagga
    33361 attaccgata cgagaaatct ggctcgtaga tgtagaagaa ggaaaagaaa agcttgagat
    33421 cgttagtcag atgtctcagc gtatgtggga tgcttctccg tatgaagtca agatctatgc
    33481 aactctggat cgggaagaag cattgaaaga tgcggatttt gttacgactc agtttcgtgt
    33541 cggtcaatta gatgcacgaa tcaaagacga gcggatccct agttattatg ggatgattgg
    33601 gcaagaaaca aatggtgctg gcgggatatt caaagctttt cgtaccgttc cagtcgtttt
    33661 ggcaatcgtt gaggacatga agcgtttatg tccaaatgct tggctgatca atttcgcgaa
    33721 cccaagtggg atggtgacag aagcagttat cagatatggc aaatgggata aagtgattgg
    33781 attatgcaat gtgccagtga tggcgatgat gacagaacca gcaatgcttg gcaagaaaaa
    33841 agaagagctg atctacaagt ttgccggtct aaatcatttc cattggcaca aagtagcaga
    33901 taataaagga aatgaccgca cggcagaatt gattgatcgt ttatacgatg aagagaatgg
    33961 attaccgaaa aatatctttg atatcccgtt tttcaaagaa caattaaaac aaatgaacat
    34021 gatcccttgt ggctaccacc gctattacta tcgtgaagaa gaaatgctgg aacatgcttt
    34081 agaagaatat caaacgattg gtacaagagc gcagcaagtg aaagaaacag aagcagagct
    34141 atttgaatta tacaaagatc cagaattaga ccataaacct gaacaattgc aaaaacgagg
    34201 tggcgcttat tattcagacg ctgcctgtga aacgattgct tcaatctatg gaaataaaga
    34261 aacacagatc gtggtatcta caaagaatga tggagctgtt cctgacctgc cggcagattg
    34321 tgtagtagaa gtcactgcct atgttggcgc acaaggagcg agaaatgtag cgtttggttc
    34381 tcttcctgct gctgaaagag gctggttgca agtaatgaag aatatggaat tgctgacgat
    34441 cgaagcagct gtgactggtg attatgggac agctttacag gcgtttacga tcaatccact
    34501 tatacgatca ggaaagacag caaaacggat catggatgag ttgtttattg cacaccagca
    34561 gtatttaccg caattcaagg agacaatcag ccgattaaaa gaagaaggaa tcgaagttca
    34621 agatgaactg gcaagagaat tatcaggtaa tacacatata atggaggaag tataatggga
    34681 ttcagaaaag attttttatg gggcggtgct acagcagcca atcaatgtga gggcggttat
    34741 gatgaaggtg gacgcggact agcaaacgta gatgttgtgc caatcggaaa agaccgtttt
    34801 cctgttatca ccggaaagaa aaaaatgttt gaatttgatg aggatcattt ttatccagca
    34861 aaaattgcaa tcgatatgta tcatcgttat aaagaagata ttgctttgtt tgcagaaatg
    34921 gggttcaaga cttatcgaat gtccatcgcc tggagtcgta tttttccaat gggggatgag
    34981 aaagagccga atgaagctgg cttgaaattt tatgaggatg cctttaaaga atgccaaaag
    35041 cacggaattg agccacttgt gacgattacg cattttgatt gtccaatgca tttgatcaaa
    35101 gaatatggtg cgtggcgttc cagagaactc gttggttttt atgaaaatct gtgtcgagtc
    35161 atcttcaatc gctataaagg acttgtcaaa tattggctga cattcaatga gatcaatatg
    35221 atccttcatg caccattcat gggcgcaggg ctgtattttg aaaaaggaga aaatgaagaa
    35281 caaatcaaat atcaagcggc acatcatgag ttggttgcaa gtgccatcgc aacgaagatt
    35341 gctcatgaag tagatccgga gaacaaagta ggctgtatgc tggcagcagg agcatattat
    35401 ccatacactt gcaatcctga agatgtttgg gcttctagaa aatcagatcg agaaaattat
    35461 ttcttcattg atgtccagtc acgaggagaa tatcccgctt atgcattgaa agaaatggag
    35521 cgaaatggca ttcagatcga aatggaacca ggggatgccg cattgctgaa agaacatact
    35581 gttgatttta tctctttttc ttattatgct tcccgagttt cgactaccga caacgaactc
    35641 ttggaacaga ctgcaggaaa tatctttgct tctgtcaaaa atccatatct gcaagcaagt
    35701 gaatgggggt ggcaaattga tccattaggc ttgagaataa cgatgaatga tatttatgat
    35761 cgttatcaaa aaccattgtt tattgtagaa aacggcttag gtgcagtcga tacgccagat
    35821 gaaaatggtt atgtggaaga tgattaccga atcgattacc tggcagcgca tatccaggcg
    35881 atgaaggatg cagtagaact agacggagtc gatctattag gctatacgac ttggggatgt
    35941 attgatttag tatcagctgg taccggtgag atgaagaaaa ggtacggatt catttatgtc
    36001 gatcgtgata accaaggaaa tggcacatta gcccgttcaa agaaaaaatc attcaactgg
    36061 tataaaaaag taattgattc aaatggagaa aatctttcga acgattgatc aatgatttat
    36121 aaaagtctat tgtttttcag cggatttttc gagtataata aagctattcg agaaacgcaa
    36181 gcagaacaat agactttttt tcgcattggc tgtaaagtat gcttacttga ttaagtaaag
    36241 gagaatctac atatgtatga tgtaatcgtt attggcgcag gtcctgctgg gatgactgct
    36301 gctttatatg cttcacgttc aaacttatcc gtattgatga tcgaacaagg tgcccctggc
    36361 ggccaaatga acaatacggc agaaatcgaa aattatcctg gattcgattc gatcatgggc
    36421 cccgatctag cttataagat gtatgaaagt gtctcaagat ttgggacaga aaatgcttat
    36481 ggaatcgttc aagatatcaa ggatcacggc agccataaag aagtcatctg tgaagataaa
    36541 agttacgaag gaaaaacagt cattatcgca acaggatgta tccaccgtaa attaggcgta
    36601 cctggcgagg aagaatttgc tggacgaggc gtttcttact gtgcagtctg tgatggtgcg
    36661 tttttccgta acaaacgtct agtagtcatc gggggtgggg actcagcagt agaagaagcc
    36721 atctatttga cacaatttgc ttctgaagta gtgatcgtcc accgtagaga tgaattacgt
    36781 gcacaaaaga tcatccaaga ccgtgcattt gcaaatgaaa aaatttcctt tatgtgggat
    36841 acagtcgttg acgaaatcgt tggaaacgaa atggtagtga caggtgtgaa agcacgtaat
    36901 gtcaaaaccg gcaatgaatt tgatttagca gcagacggag tattcatcta tgttggactg
    36961 gatccattaa ctgcaccttt tagagaagca ggtttgacga acgaagctgg ctggattcca
    37021 acagacaacg aaatgaaaac gaatatgcca ggcgtttttg ccatcggaga tgttcgtgaa
    37081 aaagatttgc gacaaatcac tacagctgtt ggtgacggcg gtattgctgg acaacaagtg
    37141 tataaatact tggagtcttt agaagaaaca acagcataag tttaaaaaga gtagaagaac
    37201 gggttcaatt gttcttttac tctttttttt cgcgtgaaat ttactgaatc tgatttatgc
    37261 gaacacgtcc aacacctttt gataaaagaa tcctttcttg atttaagcgt tttttggtcc
    37321 tcatactttc atatctaata agtgaatgct atacttatta agaaccatat ttcagaaaga
    37381 ggtgttattt tcatgtcttg ggaacaagtg tatgaacaat gggtacatga agaaaattta
    37441 gaagaaaact taaaaaaaca attaaatgat ctgggtgaag atcctgaaaa acttgaagat
    37501 gctttttatg caccgttgga attcggtact gcaggtatgc ggggaatctt aggacctggg
    37561 atcaatcgaa tgaacatcta tacggtaaga caagcaacag aaggactagc acgtttcatg
    37621 gatacacaag gtgaagaaac aaaacgccga ggtgtcgcta tcgcttatga ctcacgccat
    37681 cagtcaccag aatttgcaat ggaagcagcc aaaacactag cacatcacaa tatcccgtct
    37741 tatgtattcg aaagtctgcg tccaacacca gagctttctt ttgcagttcg tcatttgaag
    37801 acgtttgcgg gaatcatggt aaccgcatct cacaacccag cggcttacaa tggatataaa
    37861 gtctacggag aagatggcgg acaaatgcct cctgcagacg cagatgcatt gacgaaatat
    37921 gtccgtgaag tcagcaatcc gctaaaagtc gaagtattat ctgacgaaga agctaaacat
    37981 agcgggttga tcacgatcat cggagaagaa gtggacgctg cttatctaga agaaatcaaa
    38041 gcagtgacga ttgatcgtga attggttgaa acaatgggga aagatttgaa acttgtgtac
    38101 acgccacttc atggaacagg gaaaatgttg ggcgaacgtg cgttgaaaca agctggattc
    38161 gaacaattcg tactagttcc cgaacaagct gtggcagatc cagatttcac tacggtcaaa
    38221 tcgccaaatc cagaagaaca ttcagctttt gaatatgcta tacgacttgg agaaaaagaa
    38281 ggagctgact tgctgattgc aacagatcct gatgcagacc gtttaggcgc agcggttcgt
    38341 ttgccagatg gttcttatga agtattgact gggaaccaaa tcggagcatt gtttgctcaa
    38401 tatattttgg aagcacatca atcaagaggc actttaccag aaaacgcagc ggtattgaaa
    38461 tcaatcgttt ccagcgaatt gcctacagca attgcggaac actacggtgc taccatgttc
    38521 aatgtgttga cagggtttaa attcattgct gaaaaaatcc aacaatttga agaagaccat
    38581 tcacatactt ttatgtttgg attcgaagaa agttatggtt atttagttaa accttttgtt
    38641 cgcgataaag acgcgatcca agcattgctt ttattagcag aagtagcagc ttactataaa
    38701 aaacaaggca aaacgttgta tgacggcttg caaacgattt ttgaaaccta tggctatttc
    38761 gcagaaaaaa cgatttctgt gacaatgagc ggacaagaag gttctgcgaa aatctctgct
    38821 ttgatggaaa aattccgtaa agaagcaccg acagaatttg ccggagcaaa agttattcaa
    38881 acagaagact ttaaagaatt gacgagaaca gaccgtgaag gaaacgtaga aaaactaaca
    38941 acaccgccat ccgacgtatt gaagtataca ttggaagaaa atggctggat cgctatccgc
    39001 ccttcaggaa ctgagccgaa aatcaagttt tatattggtg taaaaggaag ctctacagaa
    39061 gacgcagccg aaaaagtagc ctcttatgaa gcgattatca aaaagatgac agaagaataa
    39121 aaaacttgag actgatggaa aaagtggtaa actttagaag tcagtctttt ttcaagttgg
    39181 aggtgaatgg attgccagac acgcaaaaat caaaagtaga gaaagtcagt caattattca
    39241 aaatgttgag tgatcccact cgtcttaaaa ttctgctata tctgaaagac ggggaacaaa
    39301 atgtgacagc aatcacccaa gcagtggaaa tggagcagtc tgctgtatcg catcaactac
    39361 gtttattaag agaaaatcat gtcgtcaagt ctcatcgcga gggaaaagcg attttgtata
    39421 gtcttgatga ttctcatgta ctggatattt tgaaccagac actcaaacat gtggaacatc
    39481 aataacgata ggtgaaaata gataaacact ggagtgtgac aacgaagaaa tttcgttgtc
    39541 acactccagt gttttttaag aaaaagacaa aaatgattaa tcgctttttt ctgttgtttc
    39601 agttccataa taactatgat cttctaggtc taaagcattc aaaataagcg aagccgtttc
    39661 ttcaatagat aaagatgcca cattgatcac tacacatcct agtttttcat aaagatcatt
    39721 cgcaaaatcg agttcagctc taattttttc gatatcggaa taagcagtat cagggtttaa
    39781 tccgtaagag cgcatccgtt ctttacggat attatttaaa atatctgggt tattcgttag
    39841 tcccacgatt tttttaggat cgatttccca cagctgtttg ggaatatgtg cttggggaat
    39901 caaaggtaaa ttagcaactt ttaaattttt atttgctaaa aacaagctca aaggcgtttt
    39961 ggaagttcgg gatacgccta gcaaaacgac atctgcttct aaaaaaccac gaggatcttt
    40021 cccatcatcg tattttacag caaattccat tgctttgatc cgtttgaaat agttctcatt
    40081 caataaatgg agggcaccag gttgccgaat tggcttgata ccagttaaac gttcaatttc
    40141 tgatatcggt ggcgtcatca catcgaaact aaataattga tgttcttcac aaaaagaacg
    40201 tgttatctcg atcagttctt gattgattaa tgtatgcagg acaagtgctt tttcttctaa
    40261 tgcatcttgc aatgcctgcc ttaatgtttt ttcatctgtc acgaacgttc tgcggaatag
    40321 gttgaactca acggtaggat actgagccat agtagcttgc gctagttttg tcgcagtttc
    40381 tccagcagag tcggagatga caaaaaaagt aacgatttct gtagaatcgg aagacatgga
    40441 taaaacctca cttgcatttt ttttatgttt attataccat actctagaat gcgtacttcg
    40501 taatcatttt gatttggagg aataagtcat gaaaaaacag acattgatcg aacaatcatt
    40561 agaaacattg aaaaataacg gttttaaata tactaaaaaa agagaagcat tgttaactta
    40621 tttagtgaag cgtaaccgct acgtatctgc taaagaagtt tttgactata tgaatcagga
    40681 attcaaaggg gtaagttatg atacgattta ccggaatctc catgattttg cagagatcga
    40741 tttactggaa gaaaccgatt taaatggtga aatgaagttt cgttttcatt gctgtcaggg
    40801 ggaaatcggc catcaccatc attttatctg tacagtgtgc ggaacaacga aagaaatcca
    40861 tatgtgtcca atgaattatt tcgaagaaca gctaggtgga tgcacgatcg aagggcatag
    40921 attcgagatt ttcggaagat gtgaaaattg tcaataagga tttaaataaa ataacatttg
    40981 ataatttaaa aaaaaagcaa gatttccttg acggtcaaaa cagcattaga tataatattt
    41041 acaggacagt cttgtttttt gagatatatt tcaataacaa ttacggttac tttttaagct
    41101 cggagggagg gaattaacat gtcaaaaaca gtcgttcgca aaaacgaatc acttgacgat
    41161 gctcttcgtc gcttcaaacg ttccgtgtca aaagcgggta ctttacaaga atctcgtaaa
    41221 cgtgaattct acgaaaaacc aagtgtaaga cgcaagaaaa aatctgaagc agctagaaaa
    41281 cgcaaaaaat tctagttctg tgatgaattg gagttggtag tgtgtcacta ctaagtagat
    41341 taaacgatga catgaaagca gcgatgaaag cgaaagataa ggaatcttta caagtcatcc
    41401 gaatgatcaa gtcatctata caaaacgagc agattaaaga aggtcacgac ttgacagaag
    41461 aagaagaact aacggtgttg tctcgcgaaa tgaaacagcg acgtgattct ttgcatgagt
    41521 ttgaagaagc aggacgtgac gatttagctg aaaaagtaaa aagtgaaatt gtaatagtcg
    41581 aaaaatatat gccggaacaa ctttccgacg aagaaattcg acaacttgtt caagaagcaa
    41641 tcactcaaac tggtgcttca tcaatgaaag aatttggcaa agttatgggt gcaattatgc
    41701 ctaaagtcaa aggaaaagca gatggaaacc aagtgaatgc gattgtgaaa gaactattac
    41761 aatcttaatt ttatcattta aactgcggac agtgattgtc cgcagttttt tgtgttcgta
    41821 aaaaaagaac taaaaaacag ctagaagaaa gaataagatt ctttcttcta gctgtttttt
    41881 tcctgtttaa tatctcttat gttaaattgt gtttgatttg gttattcttc tggtagttca
    41941 tttgcttctt gtttataccc tgataggcga taggcgtaat aaaagagtaa gctgccagcg
    42001 taaccaagta acgttgggac catccagcca agtcctaatg cagcaaaagg aacgtgatga
    42061 tgataaaatc ctagtaagct tgctacgaca gtttgctgac tcactaatgc aggcgaattt
    42121 gccaacatat ccaatacggc tggaacggca gaaaagaaca tcgttacttg atagacaaat
    42181 ggactctttt ggaagaattt tgctgttaag gaaagcaaga ttaacgcaag tgacaatgga
    42241 tacaaaagca tcaatactgg taaagaccat tggatgatat tatccaatcc ggcattcgca
    42301 acgacaaaag agacaaaagt cgttaaccgc agccatgtca tataactgac tttagggaac
    42361 agtttatgga aatcttgtgc aaaagaaaca acgagtccca tagcggtagt gaagacacct
    42421 aatgtgacca ttacccctaa gaagctagca ccgaagttac ctaaataaaa attagtgatc
    42481 tgagaaagtg cagttccgcc attttctgat aaaggcatct tattcaaact catcgcaccg
    42541 agaaggacca gaccaaaata aatcatgact tctaacgcaa cgctcaggct accagctttt
    42601 gctgttaatt ctgtgacttt tttatcttta aaaccaagat cacgaactgc atggacaaaa
    42661 gtgacactta aagcaagtaa agctactgca tcaaccgtgt tgtatccttc taaaacgcca
    42721 tttgtcaaag cgttcgtctg ataagaatct gcagcagatt ggtttagatt ccccatgggt
    42781 tggaataaag aaacgacaaa aatgatagct aataatgcaa gaaacaagga gtttaaatac
    42841 tttccgacat atttgaccaa acgtgttgga tgtacagtaa ggaaataagc aagtaaaaag
    42901 aaaagagcag agaataaaaa catacctaat gaatcatatt tttcaggcaa aaagggttta
    42961 gcagccattt caaaagcagt tgctgctgtc cgcggtgtac caaagaatgg tccgatcgtt
    43021 aaatgaacaa ggatcaagaa caaggcagca taccattttc cgacaggttt agctaagtca
    43081 tacagaccat cactttttgt cactactaca gctaagatag ctaataatgg gaaaagagcg
    43141 cctgagatgg caaatcctac accagcgctg atccagtttg atccagccat ttgacctaag
    43201 tggacaggaa agatcagatt acctgacccg aagaacatcc cgaagataag tgaactgacc
    43261 atcaataatt gcttccatga ataagttttt gtttcttcca taaatttttc ctccaataat
    43321 tatcattttg agtaacaaaa taaacgttgc agaaactaga aatgccacta atatcagatt
    43381 ccatgaaaac caccacctta aaatataaaa aaaagccctt ttccacttgg gaaaagggct
    43441 ctagattcta gaacggtacc accttttttc gcttaaaaaa gcttcttcat tcacggacgc
    43501 ttttcgataa agttatccgc tagcctgata atggaggcag ccacaatgat ctacgttttc
    43561 gacattgact cggaaatgat gttcgtctat tcgatttatt gtcatttctc acttacatga
    43621 ctcactggga ataaatcgct gaatagccta ctgttttcct cgacgctttg ctgtatattt
    43681 atttgttgac gttataatac acctgttttt tttgaaatgc aagaatattt caaatatttt
    43741 gttttctttt tttgaaacaa gaaaaatacc cagagaatac ttgctaagtt agacggaata
    43801 cttgtcctct atcttatgca tagcttacat gatcagcttt ttttattcaa acgagacctt
    43861 tagagaatgg aagcagctat tttttaacgt ttcttggtac ttatggttaa gcaactcttt
    43921 tagaaccttt tcttatttgc tatatagttt cttctgagta tggtaaaata ggaaagaatg
    43981 aatcgaaaaa aaggggatgt ctatttttga cggaagagtc tagttcttta gagattagat
    44041 tgacggctgc tgatgatgtc agcatgcttt taggtgcaca cgacaagcat ataaaactga
    44101 ttgaagaaac aacagaaaca accatccata cacgtggaga aatcattcag atcattggcg
    44161 aacaatccca gatttccttg gctgcctcag tcattcggac attgcaggaa ttgatcaaac
    44221 gaggaatcca tgtaagtaca ccagatgtcg tgacggcact gaaaatgggg caaaaaggca
    44281 cattagatta ttttgttgaa atgtatgagg aagagatcgt caaagaccgt aatggaaaac
    44341 caatacgggt aaaaaacagc ggccaaaaga aatatgtcga atcggtagcc aaacatgata
    44401 tcgtatttgg cgtaggacca gccgggacag gaaaaacttt tcttgcagta gtattagcaa
    44461 tcgctgcctt gaaaaaaggt gaagtgcaaa aaatcatttt gacgcggcct gctgtggaag
    44521 caggggaaaa tctaggcttt cttcccgggg atctaaaaga aaaagtagat ccttatctgc
    44581 gtcctgttta tgatgcttta taccaaattt ttggattaga tcatacgaac cgattgatgg
    44641 aacgaggcgt gatcgaaatt gctcctttag cttacatgcg ggggcggaca ttggatgatg
    44701 cattcgttat tttggatgaa gcacagaaca cgacagttgc tcagatgaaa atgttcctga
    44761 cacgtcttgg ttatcaatca aaaatgattg tcaatggaga tacgagtcag attgatctgc
    44821 ctcgagggac aacaagtggt cttgttcatg cagaacgaac attaaagcaa atcaagaaaa
    44881 tcgattttgt taattttgaa gcaagtgacg ttgttcggca ccctgtagtt gccgaaatca
    44941 tcaaagctta tgaaaaggca gatctccatc aagaataagc tttgcttagg aggataagca
    45001 catgttgaat aaaacactgc agaaactaca agaaaaactt ggaaaggcag cattgccttt
    45061 cctgctgttc ttgtttttta tcattatctg cggcgttaca tttagtagtg tcaaacaaaa
    45121 ggccaatgat ttcagagaag gccaagtagc agaagaaagt atccgtgcga acaaaacgat
    45181 cgagaacacg gaagaaaccg aacaaaaaag aaaactggca gcagaagcag tcactccaga
    45241 atacacttac caaaaagatc ttgcagatga ccagaacaat cggatcaagc aactatttga
    45301 attgatcgat aaaacgaatg atgccatcaa caaatcttat gacgaaaaga ttgataaggc
    45361 aaaggaagat gaaaatatcc caaagcctac tacagaagag cggatcgccg cgttgaagaa
    45421 aaattttgaa aatgtgaatg cagaaaatgt tgcgttttat caaaaactac cgaacaattt
    45481 ttattcgacg atatttgaaa tgactgaaac tgagatccat actgtgaggg atgagagtct
    45541 gaagctcatt gacgaacaga tgagcaaaca agttcgtgaa agtgaattgg aagcttttaa
    45601 acaagaagca gaagagcaaa tccaatattt gaatgtcaca ccagcacaac aacaaatgat
    45661 tcgctactta gtggatcaag gtatcgtagt gaatgacgta ttgaatgaga aaaagacaga
    45721 agaattgaaa caatccgcac gagaagctgt acaaccagtg atgatcttcc aaggagaaat
    45781 cattgttcgt gaaggtaatc aaatcgatgc ttcagctatg aaaaaattgg aattactagg
    45841 actgactaat caaacaactt cgatttttcc gctaattgcc atggttttag ctgttttgct
    45901 tcaaatcggc gtactgattt ataattcact tcaattccaa aatgcaggag aaagaacaaa
    45961 atacgtgctt ttctatgtga cagctatgtc agtcagcata gtgctgatga aatttttcca
    46021 attattccaa acggaacaag ctacttatat cccgctgttt tatcccgctg cttttgcacc
    46081 acttgtttta agctttttct tgaaccgaag agcagggatc atggctggta ttttccaagc
    46141 agtatctgca cttttcattt tttatgattc gattggcacg aactttctaa cgattatctt
    46201 gatgtcttat gttttctctg ggctcatggc aacgattgtc agacgcaaac ggatctcaga
    46261 acaaggatta tctgctgcca tgtgggtcat tatttttcct gtgatgatgg atgtcatttt
    46321 gatcgtctat caaggaatga gctttggtga tgcaacgact tggttaatgc ttgtctgcgg
    46381 gttcactggt gcactcttct cattcttgct gacgatcggt ctacacccat atattgaatt
    46441 gatggtcaat gatgacagtg taattgtatt aaatgaactc agcaatccaa atcatccttt
    46501 attgaaacag ttgctggaag aagcgccagg tacttatcat catagtatga tggtagctaa
    46561 tctaagtgct aacgctgtag cagaaatcgg cggacgttct ttgctaactc gagtagcttg
    46621 ctattatcat gatatcggca agatcaaaca tgctagcttt ttcgttgaga acttgccttc
    46681 aggagcggaa aacccgcaca atttcttatt gccagaagac agtaaacata ttatctttgg
    46741 acatgtgaca gatggagcca aaatattgga agattataat atgccgcaaa tggtgatcga
    46801 tatttgcaaa cagcatcatg gaaccacgct aatgcgttat ttctacatca aagcgaaaga
    46861 acgcaatccg gaagtaacag aagagcaatt ccgttatcct ggaccaaaac cgcaaagtcg
    46921 tgaagcaggg atcgtcaata ttgctgacag ttgtgaggct gcagtacggg caatggatca
    46981 tccgacgagc gaaaaaatcg aaagttttgt ccataatctt atcgaagagc gtatttccga
    47041 tggtcaatta gacgatagtg gactgacctt gaaagaaatc cgaaaagtgg aaaaatcatt
    47101 gatcagcggt ttaagtagca ccttccattc tcggatcaaa tatcctaaaa tgaaaacaga
    47161 agttgagaag ataaaggaag aacaagaaga atagaaagaa gcgatgtata atggatatta
    47221 cctttatgga tgaaacaaac caagtctcag aagagaaaat gaaagaaatc gatgacttgc
    47281 tgcaatttgc agcatcttat ttaaagctgc cagaggaaac agaaatgtct gttactttca
    47341 tggacaatgc ggcaatccaa gtaatcaatc gtgattaccg tggaaaagat gcaccgacag
    47401 acgtcatcag ttttgctctg gaagaagaag gcgaagacga aatcccagtg atttttgaag
    47461 atggagagaa tccattgcct cgtgtactag gagacatcat gatttctatt gaacgtgctg
    47521 ctgaacaagc agaagcttac ggccattcat atgatcggga acttggcttt ttagcggtac
    47581 atggattctt gcatatcaac gggtatgacc atatgacacc agaagatgag aaagaaatgt
    47641 ttggtttaca gaaagagatc ttggatgcct atggactcaa aagataaaaa cgtagagaaa
    47701 aacaaacatt tcctgatgtc tgtggaattt gctgtccaag gagtcaaaac cgtttttgag
    47761 gaagaacgca atatgaggaa acatgttgcg ttcggtatcc ttgctttatt tatgggcgtg
    47821 attttccagc ttgatcggat agagtggtta tggctgcttt tagctgtttt tctcgtttgg
    47881 atagtagaga tcatcaatac tgtttttgaa aatgtagtgg acatgtttac ggattttcat
    47941 tttcatccga ttgggaaaaa aatcaaagat atggcagctg gagcagtttt agtgaccgct
    48001 tgttttgctg tcatcattgg gttgatttta tttgtcccta aaatatatca tttgttgttt
    48061 aattaaaaag gagaacgtat atgccagaac ataaatcagg gtttgtcgca atcgtcggcc
    48121 gcccgaatgt aggaaaatcc acattattga accgaatcgt gggtcaaaaa attgccatta
    48181 tgagtgacaa ggcacagaca actagaaata aaatccaagg agtatatact acaccagaag
    48241 ctcagctgat ttttatcgat acaccaggaa tccataaacc aaagcatcgt ctaggagatt
    48301 ttatggtaga agcagcttac agtgcgttga aagaagtgga tgcagtctta tttatgatca
    48361 gtgctgatca aaaacgcggc cggggagacg attttattat tgaacgtcta aaaaacgtgc
    48421 attctccagt ttatctgatt atcaataaaa tcgataagat ccatccagac gagttgctgg
    48481 gaatcatcga agattattcg acgcagatgc cttttgcaca agtgattccg atctctgcta
    48541 cagaaggaaa caatgtcgaa cgcttaatgg atgtattagt cagtgaaatg ccagagggcc
    48601 cacaatattt tccagatgat caagtgactg atcatcctga atactttatc gtttctgaat
    48661 tgatccgtga gaaagtcttg tttttaacac gagacgaagt gcctcattct gtcgcagtcg
    48721 ttgttgattc gatgaagcga aacgagaatg ataaaatcca tatccaggcc acgatcatcg
    48781 tcgaaagaga tagccaaaaa ggaatcatta tcggcaaagg cggaaaaatg ctgaagcaag
    48841 taggaacgaa agcaagaaag gatatcgaaa atcttttagg agacaaagta tttctagaac
    48901 tttgggtcaa agtccaaaaa gactggcgag acaaaaaagt ctatctgcaa gactttggct
    48961 accgaaaaga cgaatactga tattctgaac ttcttatagg ttaagtatag aaataaactg
    49021 gataaaaatc cgcacgttga gaaaaacggc ggaagatcga aacataaata atctggatcg
    49081 gcagaatctg cttttccaga tttatttttt aggagggaga atatgagcca gcttgcagaa
    49141 tcgaaaggct tgattttatc tagccgtaat tacaaagaaa aagataaatt agtcaaaatc
    49201 tttacggaat cttctggcaa aatgatgttt tatatcaaag gaatccatcg gcaaaatcag
    49261 ccattagctc cggctttgct cccctttacc gaagcagtct atatcggtaa ttttcgaagt
    49321 gaaggtttgt cgtttttaag cagtgtcaaa gacgtccatg cgtttcggaa catccaagaa
    49381 gatatctttt tgagcggtta tggtacctat ttgctaaatc tagtagatgc agcaatcgaa
    49441 gatcatcaat atgacccgca tttattccaa tttacgcaac aagctctgca gcggatggat
    49501 caaggagatg atgcagaaat catcacgaat atctttgaag ttcaaatact gcaacgcttc
    49561 ggaatcgcgc caatttggac gcactgtgtc gtatgcgggg aaacaaaagg aaaatttgac
    49621 tattcttcca aatctggcgg tgtgatatgc gagaaacact ggccaatgga cgaacaccgt
    49681 tatcatgcag atcctcgcgc tgtttatttc gttcgaatgt tttcggctat ttcttatgat
    49741 aagatcagtg gaatccactt gaaagaagaa acgaagcagg caatccgtga cctcattgac
    49801 cagttgtatg atgaatatgt cggtattcat ctaaaaagca aaaagttcat tgatcaaatg
    49861 aagtcttggg cacacgtatt aaagacgcca gagaaaaata tgcctgaaaa agagatacct
    49921 gaaaaagaag aataaatgaa agatacaaca aaaaaatcaa aactatttga caaatacagg
    49981 gacttccgtc tattattaac cgtagattaa atagatcgtt cactgagagg atctattata
    50041 taaacaagaa aactgcaaaa tgaatacgta cattgaccaa gatcagtaaa aaacacgtgg
    50101 tttagcgagt tcgggagagt ggaagccgga cccaaacgat ttttgaagag gcacttgcga
    50161 gtacgataaa tgaagctgcc gaatctttat tcggaagtag ggtggaaccg cgaaattatt
    50221 cgtccctatg tctgtaatca gacataggga ctttttgtcg ttttctttcc tgtattctta
    50281 ttgacagcaa cgcttaaaaa ttgaaagggg caaaacagat gagtaaaaaa ttgaccgtcc
    50341 aagatatgat tttaacatta caaaaatttt ggtcatcaaa cggctgcatg ctgatgcaag
    50401 cttatgacac agaaaaagga gcaggtacga tgagcccata tacattctta cgggcaatcg
    50461 gaccggaacc atggaatgct gcttatgtag agccatcccg ccgaccagct gacgggcgtt
    50521 atggagaaaa cccaaaccgt ttgtatcagc atcatcaatt tcaagtcgta atgaaacctt
    50581 cacctgaaaa tatccaagag ctttatttag aaagcttgcg tcttttagga attgatccat
    50641 tagaacacga tattcgtttt gttgaagaca actgggagaa cccatcgatg gggtgtgccg
    50701 gtgtgggttg ggaagtatgg cttgatggga tggagatcac acaattcacg tacttccagc
    50761 aagtcggagg actaccttgt aaaccagtta cttctgagat cacttatgga ctagaacgtc
    50821 ttgcttcata tattcaagaa gttgaaagtg tctatgatct ggaatgggct gatggtgtga
    50881 aatatggcga gatcttcaaa caaccagaat atgaacattc caaatattct tttgaagtaa
    50941 gtgaccaaga cttgctacta agcaactttg accgttttga aaaagaagct aaacgctgta
    51001 tcgatgaaaa tcttgttcat ccagcgtatg attatatttt gaaatgcagc catacattca
    51061 atttattaga tgctcgcggt gcagtatccg taacagaacg agcaggttat ctttcccgta
    51121 tccgcaacat ggctcgtgct gtagccaaaa tctttgtggc agaacgcgaa aaattaggat
    51181 tccctttatt gaataaaaac acagcaaatc cagaaaacat gaaggaggct gaataagatg
    51241 gcaaaagact tattacttga aatcgggctg gaagaaatgc ctgctcatgt tgtcacacca
    51301 agcatgaaac aattagaaga aaaaacagca aaatttttag atgaaaacca attgacttat
    51361 gatacgatcg aaacgttttc tacaccgcgc cgcttagcaa tcaaagtaac aaacattcca
    51421 gaacgtcaag cagacagtga agaagaagtc aaaggaccag ccaaaaaaat tgctttggac
    51481 gcagatggaa actggacgaa agcagctgaa ggatttgtcc gcggacaagg tctgacgaca
    51541 gatgctatca cttttagaga attaaaaggc gtggaatatg tctatgtaac gaaatacact
    51601 cacggcaaag aagcgaaaga agtattgaca ggtttagcag atgtcattaa aagcctcact
    51661 ttcccagtaa cgatgcattg ggcagaatac gattttgaat atatccgccc tattcactgg
    51721 atcgtggcat tgttagatga cgaagtaatc ccgtttgaga tcttagatgt caaaacaggc
    51781 agagcctctc gaggacatcg tttcttaggc gaagatgtga catttgcgca tgcaaaagaa
    51841 tatgaagaaa aattgaatga acaattcgtt attgcttcac cgcaaaaaag acaagaaatg
    51901 atcgttacac aagcaaatga attagccgaa aaaaatcact gggttattga tctagacgaa
    51961 gatttactag aagaagtcaa caatttagtc gaatatccaa ctgtcttttc tggcgacttc
    52021 gaagaaaaat atctttctgt tcctgaagaa gtactagtca cttctatgaa agaacatcaa
    52081 cgttatttcg aggtacggaa cgatcaaggt atgctgcttc ctcattttat ttctgctcgt
    52141 aatggtgatc gggtaaaatt ggaaaatgtg gttcgaggca atcaaaaagt attgacggct
    52201 cgtttagaag atgcggaatt cttttataat gaagacaaga aagcttcaat cgaccaatac
    52261 gttgaaaaac taaaaactgt tactttccat gaaaaaatcg gtagtatcta tgagaaaatg
    52321 cagcgggtac aagtcattgc ccgtatcatt ggcgaacaag taggactatc tgaggaagaa
    52381 ttgatggatc tgcaacgagc tgctgaaatc tataaatttg atctagtaac caacatggta
    52441 ggggaatttc ctgaactaca agggatcatg ggcgaaaaat atgctctgct gaaaggcgag
    52501 aaacctgctg tagcaacagc gattcgtgag cattacatgc ctatatccag tgaaggagag
    52561 ctacctcagt ctacagtagg ttctgtttta gcgattgcag ataaattaga tagcattttt
    52621 tctttctttg cagttgggat gattccaagc gggtcaaacg atccttatgc cttacgtcgc
    52681 caagcatacg gtgtcgttcg gatcatcgaa tcgaaaaaat gggcctttcc tttgtctgtt
    52741 ctgcaagaaa cgattagtga tgttatcagt aaagatacgg atcgtttcgg cattggttta
    52801 tcagaagggc aacaacaggt gatcgacttt atcaaaggcc gtctgcgcca attattgaca
    52861 acgaagaata ttcgtcatga tgtcatcgaa gctgttttga atgcagaaca aaaagatctg
    52921 acaaaagtat ttgcagcagc acaattgttc aagcagcatc ttgcagatga agacttcaaa
    52981 ccatcgatgg aagctttgac acgtgtcgtg aacttagcga aaaaagcgga gcttgaaaaa
    53041 caatcagaag tagaccctga actgttcgaa aatgaagcgg agaaagaact gcacaaagca
    53101 gtagaggcaa tgaaaaaaag ctttgctgac caaacgatca acgaaaatta tgatcagctt
    53161 gtaagccttc gcccactgat cgaaaattat tttgatcaga cgatggtcat ggcagaagaa
    53221 ccagcagtac gtaagaaccg attaacgcaa ttaagtcaaa ttgctgccat ggctttgtcg
    53281 ctagcaagtt tagatcaaat catcacaaaa taatttctaa tatgtaaata aaccagagaa
    53341 taggcacaaa a
//
LOCUS       DARDHA010000004        50093 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000004 DARDHA010000000
VERSION     DARDHA010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.004"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(69..1634)
                     /gene="guaA"
                     /locus_tag="SDA10_000165"
     CDS             complement(69..1634)
                     /gene="guaA"
                     /locus_tag="SDA10_000165"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEG4239100.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(1693..2616)
                     /locus_tag="SDA10_000166"
     CDS             complement(1693..2616)
                     /locus_tag="SDA10_000166"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEG4239101.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            2812..3417
                     /locus_tag="SDA10_000167"
     CDS             2812..3417
                     /locus_tag="SDA10_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG4239102.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            3725..4741
                     /locus_tag="SDA10_000168"
     CDS             3725..4741
                     /locus_tag="SDA10_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG4239103.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            4882..6183
                     /locus_tag="SDA10_000169"
     CDS             4882..6183
                     /locus_tag="SDA10_000169"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEG4239104.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            6229..6891
                     /gene="deoC"
                     /locus_tag="SDA10_000170"
     CDS             6229..6891
                     /gene="deoC"
                     /locus_tag="SDA10_000170"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEG4239105.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            6912..7310
                     /locus_tag="SDA10_000171"
     CDS             6912..7310
                     /locus_tag="SDA10_000171"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEG4239106.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            7376..8458
                     /locus_tag="SDA10_000172"
     CDS             7376..8458
                     /locus_tag="SDA10_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG4239107.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            8670..10232
                     /locus_tag="SDA10_000173"
     CDS             8670..10232
                     /locus_tag="SDA10_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4239108.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            10225..11358
                     /locus_tag="SDA10_000174"
     CDS             10225..11358
                     /locus_tag="SDA10_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4239109.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            11358..12314
                     /locus_tag="SDA10_000175"
     CDS             11358..12314
                     /locus_tag="SDA10_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4239110.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            12413..13579
                     /gene="deoB"
                     /locus_tag="SDA10_000176"
     CDS             12413..13579
                     /gene="deoB"
                     /locus_tag="SDA10_000176"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEG4239111.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            13603..14421
                     /locus_tag="SDA10_000177"
     CDS             13603..14421
                     /locus_tag="SDA10_000177"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEG4239112.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            14438..15145
                     /gene="deoD"
                     /locus_tag="SDA10_000178"
     CDS             14438..15145
                     /gene="deoD"
                     /locus_tag="SDA10_000178"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEG4239113.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(15443..16129)
                     /gene="gpmA"
                     /locus_tag="SDA10_000179"
     CDS             complement(15443..16129)
                     /gene="gpmA"
                     /locus_tag="SDA10_000179"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEG4239114.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(16301..16882)
                     /locus_tag="SDA10_000180"
     CDS             complement(16301..16882)
                     /locus_tag="SDA10_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG4239115.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(17015..17698)
                     /gene="rpiA"
                     /locus_tag="SDA10_000181"
     CDS             complement(17015..17698)
                     /gene="rpiA"
                     /locus_tag="SDA10_000181"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEG4239116.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            18017..18430
                     /gene="rpsL"
                     /locus_tag="SDA10_000182"
     CDS             18017..18430
                     /gene="rpsL"
                     /locus_tag="SDA10_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEG4239117.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            18514..18984
                     /gene="rpsG"
                     /locus_tag="SDA10_000183"
     CDS             18514..18984
                     /gene="rpsG"
                     /locus_tag="SDA10_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEG4239118.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            19078..21165
                     /gene="fusA"
                     /locus_tag="SDA10_000184"
     CDS             19078..21165
                     /gene="fusA"
                     /locus_tag="SDA10_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG4239119.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            21337..22524
                     /gene="tuf"
                     /locus_tag="SDA10_000185"
     CDS             21337..22524
                     /gene="tuf"
                     /locus_tag="SDA10_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEG4239120.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            22931..23239
                     /gene="rpsJ"
                     /locus_tag="SDA10_000186"
     CDS             22931..23239
                     /gene="rpsJ"
                     /locus_tag="SDA10_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEG4239121.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            23270..23899
                     /gene="rplC"
                     /locus_tag="SDA10_000187"
     CDS             23270..23899
                     /gene="rplC"
                     /locus_tag="SDA10_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEG4239122.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            23927..24550
                     /gene="rplD"
                     /locus_tag="SDA10_000188"
     CDS             23927..24550
                     /gene="rplD"
                     /locus_tag="SDA10_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEG4239123.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            24550..24840
                     /gene="rplW"
                     /locus_tag="SDA10_000189"
     CDS             24550..24840
                     /gene="rplW"
                     /locus_tag="SDA10_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEG4239124.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            24879..25712
                     /gene="rplB"
                     /locus_tag="SDA10_000190"
     CDS             24879..25712
                     /gene="rplB"
                     /locus_tag="SDA10_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEG4239125.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            25755..26033
                     /gene="rpsS"
                     /locus_tag="SDA10_000191"
     CDS             25755..26033
                     /gene="rpsS"
                     /locus_tag="SDA10_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEG4239126.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            26056..26403
                     /gene="rplV"
                     /locus_tag="SDA10_000192"
     CDS             26056..26403
                     /gene="rplV"
                     /locus_tag="SDA10_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEG4239127.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            26417..27073
                     /gene="rpsC"
                     /locus_tag="SDA10_000193"
     CDS             26417..27073
                     /gene="rpsC"
                     /locus_tag="SDA10_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEG4239128.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            27076..27510
                     /gene="rplP"
                     /locus_tag="SDA10_000194"
     CDS             27076..27510
                     /gene="rplP"
                     /locus_tag="SDA10_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEG4239129.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            27500..27688
                     /gene="rpmC"
                     /locus_tag="SDA10_000195"
     CDS             27500..27688
                     /gene="rpmC"
                     /locus_tag="SDA10_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEG4239130.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            27713..27979
                     /gene="rpsQ"
                     /locus_tag="SDA10_000196"
     CDS             27713..27979
                     /gene="rpsQ"
                     /locus_tag="SDA10_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEG4239131.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            28037..28405
                     /gene="rplN"
                     /locus_tag="SDA10_000197"
     CDS             28037..28405
                     /gene="rplN"
                     /locus_tag="SDA10_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEG4239132.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            28442..28750
                     /gene="rplX"
                     /locus_tag="SDA10_000198"
     CDS             28442..28750
                     /gene="rplX"
                     /locus_tag="SDA10_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEG4239133.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            28778..29317
                     /gene="rplE"
                     /locus_tag="SDA10_000199"
     CDS             28778..29317
                     /gene="rplE"
                     /locus_tag="SDA10_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEG4239134.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            29336..29521
                     /locus_tag="SDA10_000200"
     CDS             29336..29521
                     /locus_tag="SDA10_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEG4239135.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            29557..29955
                     /gene="rpsH"
                     /locus_tag="SDA10_000201"
     CDS             29557..29955
                     /gene="rpsH"
                     /locus_tag="SDA10_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEG4239136.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            29987..30523
                     /gene="rplF"
                     /locus_tag="SDA10_000202"
     CDS             29987..30523
                     /gene="rplF"
                     /locus_tag="SDA10_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEG4239137.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            30684..31040
                     /gene="rplR"
                     /locus_tag="SDA10_000203"
     CDS             30684..31040
                     /gene="rplR"
                     /locus_tag="SDA10_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEG4239138.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            31061..31561
                     /gene="rpsE"
                     /locus_tag="SDA10_000204"
     CDS             31061..31561
                     /gene="rpsE"
                     /locus_tag="SDA10_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEG4239139.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            31576..31755
                     /gene="rpmD"
                     /locus_tag="SDA10_000205"
     CDS             31576..31755
                     /gene="rpmD"
                     /locus_tag="SDA10_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEG4239140.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            31799..32239
                     /gene="rplO"
                     /locus_tag="SDA10_000206"
     CDS             31799..32239
                     /gene="rplO"
                     /locus_tag="SDA10_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEG4239141.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            32239..33534
                     /gene="secY"
                     /locus_tag="SDA10_000207"
     CDS             32239..33534
                     /gene="secY"
                     /locus_tag="SDA10_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEG4239142.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            33596..34243
                     /locus_tag="SDA10_000208"
     CDS             33596..34243
                     /locus_tag="SDA10_000208"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEG4239143.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            34434..34652
                     /gene="infA"
                     /locus_tag="SDA10_000209"
     CDS             34434..34652
                     /gene="infA"
                     /locus_tag="SDA10_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEG4239144.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            34685..34801
                     /gene="rpmJ"
                     /locus_tag="SDA10_000210"
     CDS             34685..34801
                     /gene="rpmJ"
                     /locus_tag="SDA10_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEG4239145.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            34819..35184
                     /gene="rpsM"
                     /locus_tag="SDA10_000211"
     CDS             34819..35184
                     /gene="rpsM"
                     /locus_tag="SDA10_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEG4239146.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            35212..35601
                     /gene="rpsK"
                     /locus_tag="SDA10_000212"
     CDS             35212..35601
                     /gene="rpsK"
                     /locus_tag="SDA10_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEG4239147.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            35681..36619
                     /locus_tag="SDA10_000213"
     CDS             35681..36619
                     /locus_tag="SDA10_000213"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEG4239148.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            36647..37027
                     /gene="rplQ"
                     /locus_tag="SDA10_000214"
     CDS             36647..37027
                     /gene="rplQ"
                     /locus_tag="SDA10_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEG4239149.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            37299..38138
                     /locus_tag="SDA10_000215"
     CDS             37299..38138
                     /locus_tag="SDA10_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG4239150.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            38114..38983
                     /locus_tag="SDA10_000216"
     CDS             38114..38983
                     /locus_tag="SDA10_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG4239151.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            38973..39770
                     /locus_tag="SDA10_000217"
     CDS             38973..39770
                     /locus_tag="SDA10_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEG4239152.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            39973..40719
                     /gene="truA"
                     /locus_tag="SDA10_000218"
     CDS             39973..40719
                     /gene="truA"
                     /locus_tag="SDA10_000218"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEG4239153.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(40780..40986)
                     /locus_tag="SDA10_000219"
     CDS             complement(40780..40986)
                     /locus_tag="SDA10_000219"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239154.1"
                     /translation="MVTEKIGNKKMIHLSTPRPQVTIFIKDEKLFFEIALCDSELNDR
                     LTTSFLNGVPEVASSKISRNFAKF"
     gene            complement(41052..43721)
                     /locus_tag="SDA10_000220"
     CDS             complement(41052..43721)
                     /locus_tag="SDA10_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEG4239155.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(43959..44147)
                     /locus_tag="SDA10_000221"
     CDS             complement(43959..44147)
                     /locus_tag="SDA10_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEG4239156.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            44417..44779
                     /locus_tag="SDA10_000222"
     CDS             44417..44779
                     /locus_tag="SDA10_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG4239157.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            44831..46513
                     /locus_tag="SDA10_000223"
     CDS             44831..46513
                     /locus_tag="SDA10_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEG4239158.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            46630..48666
                     /gene="recG"
                     /locus_tag="SDA10_000224"
     CDS             46630..48666
                     /gene="recG"
                     /locus_tag="SDA10_000224"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEG4239159.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            48715..49716
                     /gene="plsX"
                     /locus_tag="SDA10_000225"
     CDS             48715..49716
                     /gene="plsX"
                     /locus_tag="SDA10_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEG4239160.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            49838..50080
                     /gene="acpP"
                     /locus_tag="SDA10_000226"
     CDS             49838..50080
                     /gene="acpP"
                     /locus_tag="SDA10_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEG4239161.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        16141 a         9222 c        10924 g        13806 t
ORIGIN      
        1 aaagtgcagt aaaaagataa gaagagccag aaacccttgg tataagagga tttctagctc
       61 tcctacaact attcccactc cactgttgct ggtggtttag aagtgatgtc atacacgata
      121 cggttgacgt gggctacttc gtttacgata cgtacagaga ttttttgcaa gacttcccat
      181 gggatacggg caaagtcagc ggtcattccg tcgattgaag ttacagcacg gataccgact
      241 gtgtagtcgt aagtacgtcc gtcacccata acaccgacag agcggatccc tggaagaaca
      301 gtgaaatatt gccagatatc acggtcaagt ccagcagctg cgatttcttc acgcaagata
      361 gcatcagatt cgcgtacgat ttctagcttg tcttcagtga tttcgccaag aacacgaata
      421 ccaagaccag gaccagggaa tggctgacgc caaacgattg aatcaggcat gcctaactga
      481 gtccctaatt cacggacttc gtctttgaat aatgtgttta atggttcgat caattcaaat
      541 tgcatatctt ctggcagtcc acctacatta tggtgagatt tgatcgtctg tgctgtttct
      601 gtaccagatt cgataacgtc agtatacaac gttccttgtg ctaggaaaga gacgccttct
      661 tcaccagcaa gttttgttgc ttcgtcatca aaaacatata cgaattcatt cccaatgatt
      721 ttacgttttt gttctgggtc agatacacct ttcaacttgt ctaagaagcg atctcttgca
      781 tcgactttga tgatgttcaa gccgaatttt ccgcctagac tttccattac ttgttccgct
      841 tcgcctttgc gtagcagacc gtggtcaaca aagatagatg tcaattggtc accgatcgct
      901 ttttgtaaaa gaaccccaac gacactagaa tcaacgccac ctgaaagacc aagcaagact
      961 tttttgtcac caacttgttc acggattttt tctacttgca tatcgataaa gttatccatt
     1021 gtccagttac cttcacagtg acaaacatca aaggcaaagt ggcgcagcaa ttcgattccg
     1081 tattcggaat gacgtacttc tgcatggaat tggataccat aaaaattacg tgttgtgtct
     1141 tggatagaag caatcggaca atctttgctt gttccgactg tttcaaagcc ttcaggaact
     1201 ttagttacca agtcaccatg gctcatccat actgtttgtt gttttggtgt ttctttaaac
     1261 aagacagctt gatcgcttgt tacttctaac aaggctttcc catattcgcg gtttttagca
     1321 ggctctacgg aaccgccaag tttgtacgtg atcaattgca taccatagca aatccctaat
     1381 acaggaatac ctaaattgaa gatctcagga tcgatcccaa atgcattctc atcatataca
     1441 ctgtttggtc cgccagaaag aatgattccc tttggattca tcgcttttac ttcttctgca
     1501 gtaatgcggt ggctcaataa ttcggaaaag actccaagat cacgaatacg tcttgtgatc
     1561 aattggttat actgactacc gtagtctaat acgataatct tttcaacgtt cgtcatatcg
     1621 gcaacgtttg tcaaatttat tacccctatc cctttaaatt ttatctcaaa atagtcaaac
     1681 tattttaaaa tgctaatatt ttcgcatgaa aatcgaatcg atgatatgat tttcagtttt
     1741 atggagaatt atatcggctc ttcctcgtgt aggcaagata tattcctgta aatttttcaa
     1801 attcacattt ttccatacat cttttgccat tttaaaggct tcttcacgat ctccgatagc
     1861 aaaacgataa taataattct ctggattttg gaaagccgta tccagtaacg caccaaaacg
     1921 atccaaatac catttttcga tcagcacggg atcggcatcg acatagacag aaaaatcaaa
     1981 aaagtcgctg acgtagattt gctgattcgc aggtaattga agcgtattga tcccctcaac
     2041 gatcaagata tcaggctgct ggatcaattc gtacttgtct ggaatgatat catacacatc
     2101 atgtgagtaa acaggtactt ttatctcttc ctgaccagat tttacacggt tcaagaaatc
     2161 gatcagcatc tccatatcat aactttcagg aaatccttta cggtccatga ttccacgctc
     2221 ttttaatata cgatttggat aaagaaatcc gtcagtcgtg atcagctgaa cgtttcttcg
     2281 tttgaaggta cgagacaaaa tcgtctgcag cagtctggct gttgtacttt ttccaacggc
     2341 tacacttccg gcgatcccga taataaaagg cggtacttct acgtatttat gaagaaataa
     2401 acctttactc aagaacaaag attcaaattc tttcatataa agataaatca gatgagcaag
     2461 aggtacatag atatctcgaa catcctgcat ggaaatgcgg tcattcaaac ttttgatatt
     2521 atctagttca tcttgtgtca aaggtgcttg cccattacga taaaattctc gccattcttc
     2581 tcttgccaca cggtagtagt tcattggatc attcatatta gcctcctgcg ttctcccatc
     2641 aatggaacta ttttaacaca aaataaacgc aaaccccacc acttaagaga gattgccgat
     2701 ctttttgtat aatctttata atagaaagaa acggagtctt attcttgact tgcgcacgga
     2761 tacataaaag gatagactgt tgtcatgaaa gaaatgagaa ggatggaaga catgacaaat
     2821 cattactatt ctgaacgccc agatacggcg cacgattttg aacaatgggc ttttgagtta
     2881 aaaggcagaa cctttcaatt tgtaacagat tctggtgtat tttcacgtga aacagtggat
     2941 tatggttcac gtgtcctgat tgatgctttc gattgggaag agttacctga tgaaggaaag
     3001 atcttagatg tgggatgcgg gtatggaccg attgggttag ctttggcatt tgcgacacag
     3061 cggtttgttg aaatggtcga cattaatgaa cgagcggtaa gtctagcgca aggaaacgca
     3121 aagcgtaacc aaatcgaaca tgttgatatc catcaatcga atatctatga agcagttcat
     3181 gaagaaacct atgcagctat cgtaagcaat ccgccgatcc gtgctggaaa aaaagttgta
     3241 catgaaattt tgacaggcgc atatcctcgg ttgaagaagg gcggtacact gacaattgtg
     3301 atccagaaga aacaaggagc gccgagtgcg caaaagaaaa tggaagaaac atttggtaat
     3361 gcagaaatcg tcacaaaaga taaaggatat tatatcatca aaagtgtgaa agaataacta
     3421 gctgaaaaat agtggagcga cgaaagagtg gaactgggcg tgcatgtacg ggtacgtatc
     3481 agttcttttt ttgttgagaa gtttttaaaa acaaagaact attttttctt caaaaagaaa
     3541 tagtaggaac agagtcgttg ttttttacga taaaataaat aagatacaga acttgtatag
     3601 aaggctgcca agtcggtaga taaaggaggt ttttatcaaa aaacatactt tccttttttc
     3661 tctattcttt tcttgggggt tttggtacac tgatagggaa tttagataga agggagccaa
     3721 taggatgaag ctgaccatca gagatatcgc agaaatggca ggcgtatcag taacaactgt
     3781 ctcacagatt ttgaataaca aaggaagcag atttagtgat aagacaagaa aaaaagtgtt
     3841 gtcgatcgtg aatgaacatc attataagcc cgattatttc gcttcaaatg tgatcaatcg
     3901 acattcaaag accatcggaa tgatcgtccc tgatgtcact gatttcttct tctccaaact
     3961 gatcgaaggt gtagagagct atttgaatcc tttgggctat gtgatcatgt tatgcaattc
     4021 tcgtcatagc caagaaaatg agatcaagta tttacaagag ctttcccacc gctcagtgga
     4081 cggaatcttg ctggccacac caaatatttt acctgagaaa tatgcattag acagcggttt
     4141 ttatcaaaaa atgccaatca ttttaatcga tcgcggattg aatcgtcggg ataacggacg
     4201 gcttatcgta aaggaatatg agggcgccta tcaagctgtt tcgctgttta ttgaaaatgg
     4261 ccatacgaag atcggcatgc tgaaggaaac cactggatac tatcaattag aagaacgttt
     4321 caacggatat cgtcatgcgc tgaaagatca tggcctgcct tttaatggga aatttgttga
     4381 acatggagag ttgactgtcc aaggtggata tactgcatca aaaaaattac tcaaacataa
     4441 agatatcacg gctatttttt gcgggaatga tgccatggcg atcggatgct atcaggcgat
     4501 cgatgaattg ggcaaaaaaa tcccagaaga tatctcagtc ataggattcg atggattaaa
     4561 gctgtcagaa tacatgattc cgcaattgac gacagtgcag cagccaagtt ttgatattgg
     4621 attttatgct gcacgtttcc tgatcgatac gatcgaattt cctcaaagaa aagttccgaa
     4681 caaagtgttc gaaacaaaat taattattcg tgagagcgta aaagtattga caaagggcta
     4741 actcggcggt atattgctta tgtaagcatt tacaaaggct agttagtgct ttttttattt
     4801 ttagtaaacc agtttactaa agtggtttac ctttcctaaa aataatgtat aataccgaat
     4861 gaagacaagg aggatcaggc aatgcgaatg gtggatctga ttgaaaaaaa gagggatgga
     4921 aacgagctgt cgaaagagga aatcgagtac atcgtcacga actatacgaa tggaaagatt
     4981 ccagattatc aagtcagtgc attattgatg gcaatttttt atcaagacat gacaaatgaa
     5041 gaaatcacga acttaacatt ggcgattgct aattctggtg atgtcatcga tttgagttct
     5101 cttgaaggaa taaaggtaga taagcattcg acaggtggcg ttggtgacac gaccacattg
     5161 attttagccc ccctagtagc tagtgtcgga gtgacagtgg ctaaaatgtc tggacgcggt
     5221 ctaggttaca caggtggtac actagacaag ctagaagcaa ttcccggatt tcagatcgag
     5281 ctttcggatg aagaatttgt acgcattgtt aatgaaagta aagtagcggt aattggtcaa
     5341 tcaggaaact tggcaccggc ggacaaaaaa ttgtatgcct tgcgtgacgt cacagcgacc
     5401 gttgattctc ttccgctgat tgccagttcg attatgagta aaaaaatcgc ggcaggagca
     5461 gatgcaatcg ttttggatgt gaccactgga gacggtgctt tcatgaagaa catcgaagat
     5521 gcaagacgtc tggcaaagac catgacgagc attgggaaac tggctaaccg agaaacagtg
     5581 gcggttatct cagatatgtc agaacctctt ggcgaagcta tcggaaatag tctagaagtt
     5641 gtcgaggcaa tcgaaacatt acaaggaaat ggtccagaag atctagtaga aatgtgctac
     5701 gccttaggaa gccaaatggt tgttcttgct ggcaaagcta agacaaccga tgaagcaaga
     5761 acgttgcttc aagaagctct ggaatcaggt aaagcgttag caaaattcaa agaaatgatc
     5821 caaaatcaag ggggagaccc agcgatcgtg gaacatccag aacgaatttt gactgcacgc
     5881 tacacaatgg agcttcctgc taaacaaagc ggagttgtgt caaaaatcgt ggcaaatgaa
     5941 ttaggaattg ctgcaatgat gctaggtgct gggcgtaaaa ccaaagaaga agatattgat
     6001 cacgctgttg gattgaaact gcataaaaaa attggtgaca ctgtgacaaa aggtgagtca
     6061 ctattgacga tctatagtaa cgacgaagaa atcacttctg tcattgaatt attatataaa
     6121 aatattgaaa ttggtgaatc ggcaatgaag ccaacgttga ttcatgacat catcacagaa
     6181 taaaggaaaa aagcggactt taccgtttta aagaaaaggg gaaaagaaat ggaactaaac
     6241 cgaatgatcg accatactat tttgaaagca gacgcatcta aagaagctgt gatgcaaatt
     6301 atcgaagaag caaaaaaata ccatttttat tctgtctgca tcaatccagc atgggttgcg
     6361 ctagcgaaag aacaattaca aggtgagcca gtagccgtat gtacagtaat cgggtttcct
     6421 ctaggagcaa acacacccga gacaaaagca tttgaaacaa cagatgccat caataacggt
     6481 gcagatgaag tcgatatggt catcaatatc ggtgctttga aatcaaagga tgatcaacaa
     6541 gtccaaaaag atatcgaagc agttgttgaa gcagcaaaag atcgtgcgct tgtcaaagtt
     6601 attattgaaa catcgttatt agatagagaa gaaatcatcc gagcttgcga aatagcaaaa
     6661 gctgcaggcg ctgattttgt caaaacatca actggtttct caacaggcgg agcaaaggta
     6721 gaggatgtcc gtttgatgcg tgaaactgta ggaccagaaa tgggtgtcaa agcttccggc
     6781 ggtatccata acgaagaaga agcaatggca atgatcgaag ctggtgctac aagaatcgga
     6841 acaagcgcag gagtagcaat cgtatctggt tcaactggtg aaggctatta attttttcaa
     6901 aggagaagct catggcagaa ctcaaacaag aatggatcaa cacagctatt gaagcgttag
     6961 ataaagccta tgttccttat tcccattttc cggtaggcgc ttgtctcgta acggaatcag
     7021 gcaagattta tcaaggcatc aacattgaga atgcatcatt tggattaacg aattgtgccg
     7081 agcgcacagc ttttttcaaa gcagtatctg aaggagaaaa aacatttacg catctagtcg
     7141 tagcggggca cacccccgat cctatctctc cttgtggtgc atgtcgacaa gtaatggcag
     7201 aattctgttc ccctgatatg ccggttacgc tagtaggaga caatggtgtg acgaaggcaa
     7261 cgaccgttcg tgcactgctg ccttatgcat ttactgaaaa agatctataa ctcatgttca
     7321 ttcagtcagc tgactgttga aaaataaaac aattatttta ggaggcttta cagaaatgaa
     7381 aaaagcaaaa ttatttggtt taggtgctgt cgcactagca gcagggttat ttttaggtgc
     7441 atgcggcaac aacggctcta ctgattcatc cggtggcaaa agctcatcag acacaacaac
     7501 tgcagcattg atcacagaca caggtggtgt agacgaccgt tcattcaacc agtcagcttg
     7561 ggaaggacta gaaaaatggg ggaaagatca aggactttca cgaggaaatg atggcttcca
     7621 atatttccaa tcttcaaatg aatcagatta catcccaaat attgaccaag cattgaatgc
     7681 tggattcaaa acgatttttg gtattggtta taaactaaaa ccagcgatcc aagaacaagc
     7741 aacaaacaat acaggtacga actttgttat tatcgatgat gtcatcgatg gattagataa
     7801 cgttgtatct gcaactttca aagacaacga agcttcttat cttgcaggtg ttgcagcagc
     7861 ttatacgact gaaacaaacg tagtcggctt tatcggcggt gtaaaaggtg aagtaatcga
     7921 tcgttttgat gctggattca aagccggtgt tgatgcaggt gcgaaagaat taggaaaaga
     7981 aatcaaagta ttgaatcaat atgctggtga cttctctgct ccagataaag gacgttcaat
     8041 tgctcaaggt atgtatgcac aaaacgcaga tatcatcttc catgcttcag gcggtacagg
     8101 taacggtgta ttccaagaag caaaatcatt gaacgaatca ggcgacaaaa aagtttgggt
     8161 tatcggtgtt gaccgcgacc aatcagacga aggcgaatac acattaaacg gcgaaaagaa
     8221 aaacttcaca ttgacatcta cactgaaagc agttggaaca gtagtagaag atttggcaca
     8281 aaaatcagcg gacggtaaat tccctggcgg cgaacacact gtttatggat tgaaagaaga
     8341 cggtgttggt ctaacagaag gacaactttc tgacgaagcg aaaaaagcag ttgatgaagc
     8401 aaaagaaaaa atcatttcag gtgacgtaaa agttccagaa actcctgaag aaaactaaaa
     8461 ccatttctat agccttgtta gacacatgtt tgacaaggct atggtttgat tagaacaaga
     8521 attgttcatt tatccaagct gccttgtgac agtttggata agggaacaac tccctgaagt
     8581 aatgtttgtt tcaaaagaag aacgctcact aagcgtgaat cttagtcaat cgttgcccta
     8641 gatagaaatc gttaggatgt gaaaaaaagg tggttcaaga aaatattgtc atagaaatgc
     8701 agaatattac caaagaattt ggaacattca aggcaaatga caacatcaat ttacaagtaa
     8761 aggcaggaga aattcatgcg ttgcttggag aaaacggtgc tggcaaatct acattgatga
     8821 acgtgctttc cggattatta gagccgacat cagggaaaat tttgatgcgt gggaaagaag
     8881 taaagatcac aagtccgacc aaagccaacc aattagggat tgggatggtc catcagcact
     8941 ttatgcttgt tgatgccttt actgtaacag aaaacatcgt gttgggaagc gagcctagtc
     9001 gcgcagggat gcttgaccat aaaaaagcac gaaaagagat ccaaaaagtt tctgaacagt
     9061 atggattgtc agtcaaccca gatgcttacg ttcgtgatat ttcagttgga atggaacaac
     9121 gggtagaaat tttaaaaaca ctttaccgag gagcagatgt actgattttt gatgagccga
     9181 cagctgtatt gacccctcaa gaaattgatg aattaatcgt gatcatgaag gaattagtca
     9241 aagaaggcaa gtcgatcatt ttgattacgc ataagttaga tgaaatcaaa gcagtagctg
     9301 accgctgtac agttatccgc cgtggaaaag gaatcggtac agtcaacgtt aaagacgtaa
     9361 cctcacaaca attagccgat atgatggttg gaagaacggt ttcattcaaa acgatgaaaa
     9421 aagaagcgaa gcctcaagaa gtcgttttgt ctattgaaaa tctagtggtg aaagaaaatc
     9481 gtggattaga agccgtgaaa aacttgaact tagaggttcg tgctggcgaa gtacttggta
     9541 tcgctggaat cgatggaaac gggcagtcgg agttgatcca agctttgact ggtttgcgaa
     9601 aggcagaaag cggacatatc aagctaaaag gggaagacat cactaataaa aaaccccgaa
     9661 aaatcactga acatggtgta ggacacgtgc cagaagaccg tcataaatac gggttagtcc
     9721 tagatatgac attatctgaa aatatcgcac tacaaaccta tcatcaaaaa ccttacagta
     9781 aaaacggcat gctgaattat tcagtaatga atgaacatgc cagagaattg atcgaagaat
     9841 atgatgttcg aacaacgaat gaacttgttc ccgcaaaagc tttatcaggc ggaaatcagc
     9901 aaaaagcaat tatcgctcgt gaagtcgacc gagatcctga tctgttgatt gttgcaaatc
     9961 caactcgtgg actggatgta ggtgcgatcg aatttattca taaacgtctg atcgaacaaa
    10021 gggacaaata caaagcagtg ttactgatca gtttcgaatt agaagaaatt ttaaatgttt
    10081 cggatcgtat tgctgttatc catgaaggag aaattgtcgg tatcgttgat ccgaaagaaa
    10141 catctgaaaa tgaattaggt ctattgatgg cgggctatac cttagaagaa gcacgaaaag
    10201 agttagaaaa gggggctggc gcacatgaat gatcgcagcg aaaaattccg taatttatta
    10261 gtgcctgtac tttcagttgt tttaggattg atcttaggcg cgatattgat ggctgctttt
    10321 ggttttcagc ccatccaagg ttattcttct atgctagatg cagcccttgg ttcgcaaaga
    10381 gggataggtg aaacgttaag gcaagcaacg ccgttgattt ttacagcatt aggtttttct
    10441 gtagctaact cagctgggtt tttcaatatt gggctttctg gacaagcact ttgcgggtgg
    10501 atcgtcagtg tctgggtggc acttgcgttt ccagatctgc caaaagttgt tttgcttccg
    10561 ttatgcgtga tcttaggtgc cttagctggt gcgatggcag cggctgttcc aggtttactt
    10621 cgagcatttt ttggaacaag tgaagtgatc gtaacaatca tgatgaacta tatcttatta
    10681 tacgtaagca catttgcttt acatgaagtg atgcctgaaa gctatcgctc aagtttggac
    10741 tcttccaata tgatcagtca aaatgcctca ctgcggttac catggttaac acaagctttt
    10801 ggcggctctc gcgtaaactc aggattattt ttagcaatca tcgcgcttat tttcgtttgg
    10861 ctgctgatga agaaaacgac tttaggattt gaaatccgtt ctgttggatt gaatcctttt
    10921 gcttctgagt atgcaggaat gagtagtaaa cgtacgatcg ttttgtcgat ggtcatttct
    10981 ggcgctttgg cagggcttgg cggtgtggtt gaaggattag gaacgtatca aaacttcttt
    11041 gttcaaacga cttcgctatc tattggattt gacggaatgg ctgtttcctt gcttggttct
    11101 ggatcagcaa tcggtattct tttatcagcg atcttgttca gtattttgaa gatcggtgga
    11161 cttggcatgc agacaggagc aggtgttccg tttgagatcg tcaatgtatc gattgctttg
    11221 attatcttct ttgtcgggat caacttcctg attcgtttcg tcatggctaa agtcttccaa
    11281 gggaaaaaac aagaagagat cgttgcgaca atagaatcga aaccaacgaa tcaaaaccat
    11341 gaagggggcg aactataatg tcgactatcg aattaattgc cttgatcctt acttctacac
    11401 tggtttattc aacaccgcta attctaacgt cactaggcgg aactttttct gaacgaagcg
    11461 ggatcgtaaa cgtagggcta gagggaatca tggtcatggg tgcttttagt tccatcgttt
    11521 tcaacttgac gatgagtcca tcacttggtt ctatgacacc atggatcgct attttagtgg
    11581 gtggcgtcgt aggtatccta ttttctctga ttcacgcagt agcaacagtc agtttgcgtg
    11641 ctgaccatat tatcagcggg acagtaatta atctgatggc acctgcttta ggtgttttct
    11701 tgatcaaagc atggtatggt aaaggtcaaa ctgataacat cacgcagaat ctggggtatt
    11761 tttctttccc aggattagca gatattccgg tcattggacc aattttcttt aaaaatacat
    11821 tcttacctgc ttattgtgct attttgattg ctgtcctagc ttgggtgatc atcttcaaga
    11881 cgaaattcgg cttgcgcctc cgttcagttg gtgaaaatcc tcaagctgcc gacacattag
    11941 gtatcaatgt atatgctatg cgttattcag gcgtactgat ttctggcttg ttaggcggta
    12001 tgggcggtgc tgtttttgct cagtcgattt ccggtaactt ttcagctggt acgatcgttg
    12061 gacaagggtt catctccatg gctgctatga tctttggtaa gtggaatcct ttaggggcaa
    12121 tgggtgcctc attgttcttt ggttttgctc aaagcttgag tatcattggc gcacaattac
    12181 cagttatttc ttcgattccg ccagtattgc tgcaaattgc tccatatttg ctgacaatca
    12241 ttgtgctggt tgttttctta gggaaagctt ctggtccaaa agcaaacggg aagaactata
    12301 tcaaatcaaa ataatctttt agcgaataaa aagggtgggt tacgggaagt gtttttcgtc
    12361 tcgccctttt tgttttaaag gcaataaaaa taaaaagaaa gaaggaataa tcatgtttaa
    12421 acgcgtacat ctgatcgtca tggattcagt aggaattgga gaagcaccag atgccgataa
    12481 gttcggtgat gttggttctg atacattagg acacattgcg aaagaagcag gattgaacgt
    12541 tccacattta gagcagcttg gtttaggaac gatccgtcct cttgaaggtg tggaatcagt
    12601 gaaagatcat caaggctatg cgacgaaatt agaagaagtt tctgtaggga aagatacgat
    12661 gacaggccac tgggaaatca tgggattgaa tatcaaaact ccatttcgtg tgtttcctaa
    12721 tggattccct gatgatctga tcaaacaaat cgaagaattt tctggccgta aagtcgtatg
    12781 taacaaacct tatagcggaa cagctgttat tgacgattac ggtgaacacc aaatgaaaac
    12841 aggcgattta attgtatata cttctgccga tcctgtcctt cagatcgcgg cacatgaaga
    12901 aatcattcct ttagaagaat tatatcggat ttgccaattc gttcgtgata taacaaaaga
    12961 tgatccgtat atgattggcc gaatcattgc acgtccgtac gtgggtgaac caggagactt
    13021 cacacgaaca agcaatcgcc atgattatgc gttgaatcca ttcggacaca cagtccttga
    13081 ttctttgaaa gaagcaggaa aggacgtgat tgctgttgga aagatcaacg atattttcaa
    13141 cggacaagga atcactgaat ctgtccgcac gaaaagcaat atggatggcg ttgaccagct
    13201 gctgaaagtt atgaaaaacg atttttcagg aatcagcttt accaatttag tagattttga
    13261 tgcattatac ggtcatcgtc gggatgtagt cggctatgcg catgcgattg aagaatttga
    13321 cttacgaatt ccagaattgc ttgaagcgat ggaagaagac gacttgctaa tgattacagc
    13381 cgatcacgga aacgatccga cgtttactgg taccgatcat acgcgagaat atgtaccgtt
    13441 attggtttac agtaaaaaaa tgaaagaaca aggggcattg cctcaaggat attattcaga
    13501 tatttcagca acgattgctg agaactttag tgtttcttcg actgaaaatg gcgaaagctt
    13561 cttgaaatta ttaaaataat ctttttaaat aggagaaagc aaatgaccaa gttaagcgag
    13621 atgttacaag aaacgaccca gtttatcctt gataaaggag taaaagaaat tgattttggt
    13681 ttgattctag gttcaggatt aggtgaatta gcagatgaaa tcgaagatgc tgtaatgatt
    13741 tcttatgatg agatcccgtt ctttcctgtt tcgacagtag ttgggcatgc aggaaagcta
    13801 gtttatgggt ctctggcagg caagaaagtg ttagccatgc aagggagatt ccactactat
    13861 gaaggtcatt ccatgcaaac agtgacctat ccagtacgtg tgatggccgg actaggggcc
    13921 cattcagtca ttgtgactaa tgcatgtggt ggtgtcaacg aatcatttgt accaggagat
    13981 ttgatgctga ttaccgatca tatcaatttt actggtgaaa atcctttgat cggtccgaat
    14041 gaagatgcaa tgggccctag atttccagat atgagtcaag cttatacgcc tgactatcaa
    14101 gcaacagcaa aagcagcagc agaaaaacta ggtttggatc ttaaagaagg cgtatatatg
    14161 ggctattctg gaccaactta tgaaacgcct gcggagatcc gaatgtctcg gacaatagga
    14221 gccgatgcag tagggatgtc gactgttcca gaagtcatcg tagcagctca cagcggatta
    14281 aaagtgttag gaatctcttg tattaccaac ttagcagcag gtatgcaagc gaatctgagc
    14341 catgaagaag ttgtagaaac aactcagaga gtcaaacaat cttttaaagc attggttaaa
    14401 gaaacattga cattattgta ataggaggaa caataaaatg agtgtgcata ttgaagcgaa
    14461 agaaggcgag atcgccgata agattttact tccgggagat cctttgcgag ctaaatatat
    14521 tgcggagact tttttggaag atccagtttg ttacaatcag gtacgcggta tgttaggctt
    14581 taccggcaaa tataaaggag aacgtatttc tgttcaagga acaggaatgg gaatgccatc
    14641 agccaccatc tatgcccatg aattgatcca gtcgtatggt gtaaagaaat tgattcgagt
    14701 aggtacatgc ggtgcattgt caaaagacgt ccatgttcgt gatttagtac tagcacaagg
    14761 tgcagcgacc agttcttcga tgatcgaaaa aaatttccaa gcctttcatt ttcctccaat
    14821 cagtgatttc aatctattac tgaaagcata tgagatagct aaagaaaagg gctatacggt
    14881 tcatgtagga aatgttcttt cggaagattc tttctacaaa gatgatttga cagagacgtt
    14941 ccaattagct gaacttggcg ttttaggagt ggaaatggaa gcagcggctc tttattactt
    15001 aggcgcgaaa taccatgtgc agacattaag tttgatgacc gttagcgacc atttgatcac
    15061 cggtgaagaa acaacagctg cagaacgtca atctacgttc aatgaaatga tcgaagtagg
    15121 attggaaaca gcgatagcag aataagaagg aaatgaaaaa gctgccctgc attaagtaat
    15181 aagaacatcc aagcaaaaat agctcctcat gtttttgact tggctggact tattattgca
    15241 aatgtaagct tttgaacacc gtttaccaag aggttgtgaa tctgccgttt aactctgagt
    15301 cacaaggaac aatttcaaaa aacagctttt catgtttgat gaatattgtg acttgtgacc
    15361 gagaagttgg caggagaaaa ccgtttatac gaaaaaaggg actgtgacaa atcgtgtcac
    15421 agtccctttt ttatagtgtt ttttataggt agtatttttt agcaactgtt aagtcatcat
    15481 ttaattcata aacaagtggt tgacctgttg gaatttcaag atccatgatg tcttcgtcag
    15541 agataccttc gatatgttta gctagggcgc gtaatgagtt accatgtgca gctactaaaa
    15601 cagttttgtt gtctagtaat gctggagcaa tttcatcttg ccagaatggc aaagcgcgtt
    15661 ctaatgtcac tttcaagttt tctccaccag gaatatcgcg tttgtctaac attgcgtaac
    15721 gacggtcatt tgctgcagaa ccttcgtcag tttcttccat taatggagga agagtatcgt
    15781 aagaacgacg ccagatgtgc acttgttcgt cgccgtattt ttcagctgtt tcttttttgt
    15841 tcaaaccttg taatttacca tagtgacgtt cgtttaagcg ccatgatttg atttgtggta
    15901 cccaaagttg atctgaatat tctaagatca agttacatgt tttgattgca cgagttaaaa
    15961 cagaagtgta agcaacatca aattcgattc cagcttcttt gattttgcgt ccgccttctt
    16021 ttgcttcttc gattccttct ggtgctagat ttacatcagc ccaaccagta aattggttca
    16081 atgcattcca ttcactaaga ccgtgacgag aaaaaactaa ttttggcatg tttagctctc
    16141 cttttaagat aggtttatat ttctgaaatt ttcagttctg ttttattgta caattttttg
    16201 gaagagattt ccatcgaaaa attagataag attctatgag aaaacaagta gagaatataa
    16261 aaaaacgaac agtgcccacg caactgttcg tttttaggta ttagatgcat aattgctcga
    16321 tttctttttc aggtaaattg atatatttat aacatgttcc aactgaaaca ccgcattttg
    16381 aagaaataaa ttggatcgtt tctttttttt cataatagag acgtcggatt tttttgatcg
    16441 tttttgcatc gatttttggt cttccgccga tttttccttt tttacgagct tcatctaatc
    16501 caactaatgt acgttctttg atcaatgcgc actccatgtt tgctaggttt tccattaatt
    16561 ggaaatagat ttttcccatt tcgcgcgtat cgattccttc atcaagacta atcaagtgca
    16621 gcccgcgatc tttgaataat tgcgtgagtt ctgttaattg ccgcgtagat ttgcctaatc
    16681 gatgaagttg gcaaatgact agtgcatcgc cttgttgcat gtcagcgatc actgactcca
    16741 aatgaacagc agtagaatca ttttgttcca cttcatatgt ctcatggaaa atttcttgac
    16801 aaccaaagtc agataattga tcaacctgag ttgttaaatc aacgtcattt atggttgtgc
    16861 gtgcataacc aataattttc atggtgattc ctttctagca gtgattttct aactggatat
    16921 aacagtaaaa atgtagcata aagtacgtca aaaagatagt tttttcgcaa attttccgct
    16981 gtcttttttt attggatgat caagcagttt ttctttattt acgacggatt gcgtcaagaa
    17041 tctcaacgcc tttttctgag ccgacaacta ctttagaaac catgtttagg aaaagcccat
    17101 gttctactac cccaacaagt tcatctaaat aggtagctaa gctttctggc tgttcgatga
    17161 cttctagatg cagatcaatg atgtaatgac caccatctgt ggttaagact tctccgtttt
    17221 cgtctgtacg caagacgggt tggaatcttt tttcttcaaa aatatgaagt agctgttggg
    17281 agccataagg gatcacttca actgggagtg ggaacttccc aagtttatcg actaatttag
    17341 aagaatcaac gatccaaatc gtttcttttg agtacgtagc gactattttc tcgaaaagca
    17401 aagcagcgcc gccgcctttg atcccttgaa aatcagcact gatttcatca gcaccgtcta
    17461 ttgtcaaatc gactacgggg acttcatcaa tactctttaa cggtattcct aatttttccg
    17521 cttgttcttt tgtcgcattt gaagtagtaa ctcctgtgat acgtaaacct tcttcacgca
    17581 tacggcgacc aatctcttct accatataat aggcggttga accggtcccg agacctacga
    17641 tcatcccgtc ctttacgaat ttcgcggctt caatgccagc taattgtttt aaattcatct
    17701 ttgcgtccac ctttttttag tttgagcatc gtgctttcct cactatttta gcgtgattta
    17761 cggtaaaaag ataccattct tttttattcc aataaagctt tttcaaaaaa aattgcatct
    17821 cttgttgaca tgttttttca ttcgtgatat tctaacaaag gtgctttatc tacaggtttc
    17881 tgtgaagaaa cgtgctgact gtttatcaaa gcatatttga taaggatgca gacattgcat
    17941 ttatttttta tcacaaaaaa agaaccacct ggatgtgtgg aactagacgc aaatcgtggg
    18001 aaggaggaaa caaggaatgc ctacaattaa ccaattagta cgtaaacctc gtaaatcaaa
    18061 ggtggaaaaa tctaattcac ctgctttgaa caaaggatat aacagcttta agaaaactca
    18121 aactaacgta aactcacctc aaaaacgtgg ggtagcaact cgtgtgggta caatgacacc
    18181 taaaaaaccg aactcagctt tacgtaaata tgcacgtgtt cgtttgtcaa acttaattga
    18241 agttactgct tatatcccag ggattggtca taacttacaa gaacacagtg ttgtattatt
    18301 acgcggtggt cgtgtaaaag acttaccagg ggtacgttac catatcgttc gcggtgcttt
    18361 agatactgct ggtgttaacg accgtaaaca aagccgttct aaatacggaa ctaaaaaacc
    18421 taaagcttaa ttttaattat taaacaaatg aaacttgctt ctactatata gaagtaatta
    18481 ctatgaacaa tatttcggaa ggaggagtta cggatgccac gtaaaggtcc tgttgcaaaa
    18541 cgtgatgttt taccagatcc tatttataac tcaaaattag taactcgctt gatcaaccgt
    18601 gtaatggttg acggaaaacg cgggattgca gctaacatta tctataatgc ctttgacttg
    18661 atcaaagaat ctacaggtaa cgatccatta gaagtgtttg aacaagcaat gaaaaacgtt
    18721 caacctgttc ttgaagttaa agcacgtcgt gttgggggtt caaactacca agtaccagtt
    18781 gaagttcgcc ctgaacgccg cgttactcta ggtcttcgtt gggtagtaaa ctacgctcgc
    18841 ttgcgcggtg aacacacaat ggaagaacgc ttagcaaaag aaatcatgga tgctgcaaac
    18901 aacacaggtg cttctgttaa aaaacgtgaa gacactcata aaatggctga tgccaaccgt
    18961 gcgtttgctc actatcgctg gtaagattct ctctgtttgg tcggctttta taagtcgacc
    19021 tcagatagtg tccttatttt tagcaactag atagtaaaga gaggagaaac aaggagaatg
    19081 accagagaat tttctttaca aaacactcgt aatatcggta tcatggccca catcgatgct
    19141 ggtaaaacga ctgctactga acgtatcttg tactatactg gtaaaatcca taaaattggt
    19201 gaaactcatg aaggagcttc acaaatggac tggatggaac aagaacaaga acgtggtatt
    19261 acgatcactt ctgctgctac tacagcacaa tggaagggtc accgaatcaa catcatcgac
    19321 acaccaggac acgtagactt cacagttgaa gttgaacgtt cattgcgtgt acttgatggt
    19381 gctgtaactg ttcttgatgc acaatcaggt gttgaacctc aaactgaaac agtttggcgt
    19441 caagcaacaa cttatggtgt acctcgtatc gtatttgcaa acaaaatgga caagatcggt
    19501 gctgacttct tatattcagt atcaacttta catgaccgtt tgcaagcaaa cgctcaccca
    19561 atccaattac caattggtgc ggaagacgac ttcacaggaa ttatcgacct tgtaacaatg
    19621 aaggctgaaa tgtatacaaa cgatcttgga acagagatcg aagaaacaga aattcctgaa
    19681 gaataccgcg aattagctga agaatggcgc gaaaaattgg ttgaagctgt agctgaaact
    19741 gatgaagaac ttactttgaa atacttagaa ggtgaagaaa tcactgaagc tgagttaaaa
    19801 gaaggtatcc gccgcgcaac agttaacgta gaattctacc cagtactatg tggttcagca
    19861 tttaagaaca aaggggttca attactattg gacgctgttc ttgattactt gccatcaccg
    19921 ttagatatcc cagctatcaa agggattgac cctaaaactg atgaagaagt agaacgtcct
    19981 gcagacgaca gcgctccttt ctcagcttta gcatttaaag ttatgactga cccattcgta
    20041 ggtcgtttaa ctttcttccg tgtgtactct ggtgttttac aatcaggttc atacgtacaa
    20101 aacgcaacaa aaggcaaacg tgaacgtgtt ggtcgtatcc tacagatgca tgccaactct
    20161 cgttcagaaa tctctgaagt ttacgctgga gatatcgctg ctgctgttgg tttgaaagac
    20221 acaactactg gggacacact atgtgatgaa aagagtctag ttattcttga atccatggaa
    20281 ttcccagaac cagttatcca agtcgctatt gaacctaaat caaaagctga ccaagataaa
    20341 atgggtgtgg ctttacaaaa actatctgaa gaagatccta ctttccgtgc tgaaacaaac
    20401 gtggaaacag gcgaaacaat catcgctggt atgggtgaac ttcacttaga tatcatcgtt
    20461 gaccgtatgc gtcgtgaatt caaagttgaa gctaacgtag gtgcgcctca agtatcttac
    20521 cgtgaaactt tccgtgctgg cactcaagcc gaaggtaagt ttgtacgtca gtctggtggt
    20581 aaaggtcaat acggtcacgt atggattgaa tttacaccaa atgaagaagg tgctggtttc
    20641 gaatttgaaa atgcgatcgt cggtggggtt gttcctcgtg aatacattcc agctgttgaa
    20701 actggtttga aagatgctat ggaaaatggt gtcttagctg gctatccatt agtagatatc
    20761 aaagctaaac tttacgatgg ttcataccat gatgtcgatt caaatgaaac tgccttccgt
    20821 gttgctgctt ctatggcatt acgtgcagca gctaagaaag ctaatccagt aatccttgaa
    20881 ccaatcatgg ctgtagaagt tgttatccct gaagattatt taggtgatgt tatgggtcac
    20941 gtaactgctc gtcgtggacg tgttgaaggt atggaagctc gcgctggcgg ccaacaagtt
    21001 gttcgtgcga tggttccact tgctgaaatg tttggttatg ctacaactct tcgttctgct
    21061 actcaaggtc gtggtacatt tacaatgaca tttgaccact acgaagatgt accaaaatca
    21121 gtacaagaag aaatcattaa gaaaaacggc ggaaaagctg aataatttct taaaaccgga
    21181 tagtcctgca aagagattat ccaaagagac tatattttta atatagtttc gtgtaaaata
    21241 attatcgatg atatgggcat gtggtgtacc ttgtgtacat catataccta tcagacaaat
    21301 aaaaaatata ttgtattttt aggaggacat tttaaaatgg caaaagaaaa atttgaccgt
    21361 tctaaaccac acgttaacat tggtactatc ggacacgttg accatggtaa aactacatta
    21421 acagctgcaa tcacaactgt actatctaag aaaaacggcg gccaagctat ggcttacgat
    21481 caaatcgatg gtgctccaga agaacgcgaa cgcggaatca caatctcaac agctcacgtt
    21541 gagtatgaaa cagatacacg ccactacgct cacgttgact gcccaggaca cgcggattac
    21601 gttaaaaaca tgatcactgg tgctgctcaa atggacggag ctatcttggt agtttctgct
    21661 gctgacggcc caatgcctca aactcgtgaa cacatcctat tgtctcgtca agttggtgtt
    21721 ccttacatcg ttgtattctt gaacaaagta gacatggttg atgacgaaga attactagaa
    21781 ttagttgaaa tggaagttcg tgacctatta acagaatacg aattccctgg tgacgatgtt
    21841 cctgtagttg ctggatcagc tttgaaagct ctagaaggcg acgcttcata cgaagaaaaa
    21901 attcttgaat taatggctgc agttgacgaa tacatcccaa ctccagaacg tgacaacgac
    21961 aaaccattca tgatgccagt tgaagacgtg ttctcaatta ctggacgtgg tactgttgct
    22021 acaggtcgtg ttgaacgtgg acaagttcgc gttggtgacg aagttgaagt tgttggtatt
    22081 gctgaagaaa cttcaaaaac aacagttact ggtgttgaaa tgttccgtaa attgttagac
    22141 tacgctgaag ctggagacaa cattggtgct ttactacgtg gtgttgcacg tgaagacatc
    22201 caacgtggac aagttttagc taaaccaggt acaatcacac ctcatacaaa attctctgca
    22261 gaagtatacg tgttgacaaa agaagaaggt ggacgtcata ctccattctt cactaactac
    22321 cgtccacaat tctacttccg tacaactgac gtaacaggtg ttgttgaatt accagaagga
    22381 actgaaatgg ttatgcctgg cgacaacgta acaatggaag ttgaattaat ccacccaatc
    22441 gctatcgaaa acggtactaa attctcaatc cgtgaaggcg gacgtacagt tggtgccggt
    22501 gtcgttacag aaatcaaagc ttaattcact taagttttaa agtaaagcaa catttattcg
    22561 gacgtaatcg ctcggaacga aggtcttagg atctttgttc cgagcttttt tatttattgc
    22621 aggatcagga aaaaagacag gagtattata aatagaagaa acacgaaata aaagagtcag
    22681 tattttctaa atgaaacttt atgaaaacaa aatgtttttt acaagaaaaa gccttgcttt
    22741 gtgagtttat atatattata atgacaaagg tgcgtttact ttagtgaatt gcacagacgt
    22801 aatcagaatg atttcgtcgg ctaagaaacg agaggttgcg acacgcccgg acgctttgcc
    22861 acgaacgagc gtgacggaaa ttttcgtgga gctatgtcta cttttcaaaa taggcgaagg
    22921 agggaacaag atggcaaaac aaaaaattcg tatccgttta aaagcgtatg aacaccgtat
    22981 tttagatcaa tcagcggata aaatcgtgga aactgcaaaa agaactggag ctagcgtgtc
    23041 tggtccaatt ccattaccaa ctgaacgcag tctttacaca atcatccgtg cgactcataa
    23101 atacaaagat tcacgcgaac aattcgaaat gcgtacacac aaacgtctta tcgacattgt
    23161 gaacccaaca ccaaaaactg ttgatgcttt gatgaagcta gacttaccat ctggtgtaaa
    23221 catcgaaatc aaattataaa actaaaacaa aaataatgga ggtgtactca tgaccaaagg
    23281 aatcttaggg aaaaaagtgg gaatgacaca aatcttcact gaatctggtg aattaattcc
    23341 agtaactgtt gtagaagcta ctccaaacgt agtactacaa gtgaaaacaa tggaaacgga
    23401 tggctacgaa gctattcaag ttggttacca agatatgcgt gaagttttat caaacaaacc
    23461 tgcgaaaggt catgttgcaa aagcaaacac ggctcctaag cgcttcattc gtgaattcaa
    23521 aaatgttgag ctaggagaat atgaagtagg aaaagaaatt acagtagatg ttttccaagc
    23581 aggagacatt attgatgtaa caggtactac gaaaggtaaa ggattccaag gggttatcaa
    23641 acgccacgga caaagccgcg gaccaatggc tcacggttct cgttaccatc gtcgtcctgg
    23701 gtcaatgggt ccagttgcac ctaaccgtgt atttaaaaac aaacgtcttg ctggccgtat
    23761 gggtggcaac cgcgtaacta tccaaaactt ggaagttgta cgtgtcgatg cagaaagaaa
    23821 tgttatcttg atcaaaggta atgttcctgg agctaaaaaa tctttaatta ctatcaaatc
    23881 agctgtgaaa gctaaataat tgtaggaaga aaggaggaac taaggaatgc cgaatgtagc
    23941 attattcaaa caagatggaa gccaaaacgg tgaaatcaca cttaacgaag aaatcttcgg
    24001 aattgaacct aatgaaagtg ttgtttacga tgcaatcgta atgcaacgtg cgtcattaag
    24061 acaaggaaca cacgcggtta aaaaccgtag cgctgttcgc ggcggcggac gtaaaccatg
    24121 gcgtcaaaaa ggaacaggtc gtgcacgtca aggatctatc cgctcaccac aatggcgtgg
    24181 aggtggcgta gtctttggac caacaccacg ttcttacagc tacaaacttc ctaaaaaagt
    24241 tcgtcgttta gcaatgaaat ctgtattgtc agaaaaagtt gctgaaaaca acttggtagc
    24301 aatcgaaggc ttgaacttcg acgcaccaaa aacaaaagaa ttcaaacaag ttcttgctaa
    24361 cttatctatc gactctaaag tattggttgt tctagaaaaa ggtaacgact ttgctgcttt
    24421 atcagcacgt aacttaccaa acgtttctgt agtaacttct gataatgtga gtgtgctaga
    24481 cgttgtatca aatacaaaag tgttagcaac acaaactgct cttactcaaa ttgaggaggt
    24541 gcttgcataa tgaacttact agacgtaatc aaacgcccag tgatcactga aaaatcaatg
    24601 cttgccatgg atgacaagaa atacactttt gaagtggaca ctcgcgcaaa caaaacttta
    24661 gtaaaacaag cggtagaagc tgcttttgac gttaaagtca aaaacgtgaa catcgtgaac
    24721 gtacgtgcga aattcaaacg tatgggcaaa tatgcaggat atacaaaaaa acgtcgcaaa
    24781 gcgatcgtta cattgactga agattcaaaa gaaatccaat tattcgaagc tgctgaataa
    24841 gcactaatag ataactcatt aaattaggag ggaaaaacgt ggcgattaaa aagtacaaac
    24901 ctacctcaaa tggtcgtcgt aacatgacag gttctgattt tgctgaaatc acaacatcaa
    24961 cacctgaaaa atcattgtta caaccattga aaaaccatgc tggacgtaac aacaacggcc
    25021 gcatcactgt acgtcatcaa ggtggcggac acaaacgcca atatcgcgtg atcgacttca
    25081 aacgtaacaa agataacgtt gttgcaactg ttcaaacgat cgaatatgat ccaaaccgtt
    25141 cagctaacat cgcgttagta cactatgaag atggagtgaa agcttacatc ttagcaccta
    25201 aaggactaga agtgggtatg aaacttgttt caggtccaga agcagatatc aaagtcggaa
    25261 atgctcttcc attagaaaac attccagttg gtactgttat ccacaacatt gaaatgaaac
    25321 ctggtaaagg tggacaattg atccgttctg ctggtacaag tgctcaagta cttggtaaag
    25381 aaggcaaata cgtattgatc cgcttgaact ctggtgaagt tcgtatgatc ttagctgcat
    25441 gccgtgctac aatcggttct gtaggtaacg aacaacacga attaatcaac atcggtaaag
    25501 ctggacgctc tcgttggatg cgtaaacgcc caactgtacg tggtagcgta atgaacccta
    25561 atgatcaccc acacggtggt ggtgaaggta aagctcctat cggacgtaaa gcaccagttt
    25621 ctccttgggg tcaaccagct cttggttaca aaactcgtaa caaaaaagct aaatcagaca
    25681 aacttattgt tcgtcgtcgc aaaaataaat aattactttg accatgtgtc gcacattttg
    25741 agaggaggtt caccatgggt cgtagtttaa agaaaggacc ttttgtcgat gagcatttaa
    25801 tgaaaaaagt tgaagctcaa gcaggggttg aaaagaaaaa agttatcaaa acttggtcac
    25861 gccgttcaac agttttccca agttttgttg gatataccat cgcagtttat gatggacgta
    25921 aacatgtacc agtttacatc caagaagaca tggtaggaca caaattaggt gaatttgcac
    25981 ctacaagaac ttatcgtgga catgctgctg acgataagaa aacgaaacgc taatttgagg
    26041 ggaggataaa tccaaatggc agaacaaatc acatcagcta aagcaactgc aaaaacagtt
    26101 cgcacttcac ctcgaaaagc ccgtttagta attgacctta tcagaggtaa aagtgtcgca
    26161 gatgcaattt caatcttgaa attcacacca aacaaatctg ctggaatcat tgaaaaagta
    26221 ttgatgtcag cagttgcgaa tgcagaaaat aactttgact tagatgttga aaacttggta
    26281 gtaagcgaag cttttgttaa cgaaggacct acaatgaaac gtttccgtcc tcgtgcaaaa
    26341 ggatcagctt ctccaatcaa caaacgtaca agtcatatca cggtagtcgt atcagaaaaa
    26401 taaggaggga taatcagtgg gtcaaaaagt acatccaatt ggaatgcgtg taggcatcat
    26461 ccgcgactgg gatgctaaat ggtatgctga aaaagagtat gctgagttct tacacgaaga
    26521 tttaagaatc cgtaaattta tttcaactaa acttgctgat gctgctgtat caacagttga
    26581 aatcgaacgc gcaacaggtc gcgtaaacat ttcaattcac acagctaaac caggtatggt
    26641 tattggtaaa ggcggttctg aagtcgaaag cctaagaaaa gaattaaaca aattaactgg
    26701 taaaagaatc catatcaata tcgtggaaat caaaaaacca gatctagatg caaaattagt
    26761 aggtgaagga atcgcacgtc aattagaaaa ccgtgttgct ttccgtcgcg ctcaaaaaca
    26821 agccatccaa cgctcaatgc gtgctggcgc tcaagggatc aaaactcaag tatctggtcg
    26881 tctaaacgga gcagatatcg ctcgttcaga aggatactca gaaggaactg ttcctcttca
    26941 tactttacgt gcggacattg attacgcatg ggaagaagca gatacaacat acggaaaact
    27001 aggagttaaa gtgtggatct atcgtggaga aattcttcca actaaaaaaa acactgagaa
    27061 aggagggaaa taatcatgtt agtacctaaa cgtgtaaaac accgtcgtga attccgcgga
    27121 aaaatgcgcg gggaagctaa aggcggaaaa gaagtagcat tcggtgaata cggtttgcaa
    27181 gctgttgatt cacattggat cacaaaccgc caaatcgaag ctgctcgtat cgcaatgact
    27241 cgttacatga aacgtggtgg gaaagtatgg attaaaattt tccctcacaa atcttatact
    27301 gccaaagcaa ttggggtacg tatgggttct ggtaaagggg cacctgaagg atgggttgca
    27361 ccagtaaaac gtggtaaaat catgtttgaa atcgcaggcg ttcctgaaga agtagctcgt
    27421 gaagcgttac gtctagcttc tcacaaatta ccaatgaaaa ctaagatcgt aaaacgtgag
    27481 gaaatgggtg gtgaatcgaa tgaaggttaa agaaatcaga gaattaacca ctgccgaaat
    27541 gcttgatcaa gaaaaacaat taaaagaaga attgtttaat cttagattcc aattagcaac
    27601 aggtcaatta gaaaacactg cacgtattaa agaagtacgt aaatcgattg cacgcatcaa
    27661 aactgtgttg cgtgaacaag ctaagtaatt atggaaggag gccattaagc gtatgactga
    27721 aggaagaaat caacgcaaag tttatcaagg tcgcgtggtt tcagacaaaa tggataaaac
    27781 aattaccgtt gctgtcgaaa caaagaaaaa ccaccctatc tatggcaaac gcatgaacta
    27841 ttctaagaaa tacaaagtgc atgatgaaaa caacacagca aaagttggcg acatcgtgag
    27901 aattatggaa actcgtccat tatcagctac aaaacgtttc cgtttgttgg aagtagtcga
    27961 agaagcagtt attatctaat aacacgagat tttcctatat agattgaatg caaaatctga
    28021 aaggaggata cacaacgtga tccaagcaga aagtcgttta aaagtcgctg ataactcagg
    28081 cgcacgtgaa attttgacga tcaaagtcct tggtggatct ggtcgtaaaa ctgctaacat
    28141 cggtgatgtg atcgttgcta cggttaaaca agcaacgcca ggtggggttg tcaaaaaagg
    28201 tgacgtcgtt aaagccgtta tcgttcgaac aaaatcagga gctcgtcgta cagacggttc
    28261 atatattaaa tttgatgaaa atgctgcagt gattattcgt gatgataaaa gccctcgtgg
    28321 aacacgtatc tttgggccag ttgcacgtga actacgtgaa aacaacttca tgaagatcgt
    28381 ttcactagca ccagaagttt tataatcttg agacacttat caaaggaggt gcgaaacagt
    28441 tatgtttgtt aaaaaaggcg ataaagttaa agttatcact ggaaaagaca aaaacaaaga
    28501 aggcgttgta ctagcagcgt tcccgaaaaa agataaagtt attgtcgaag gtgtaaatgt
    28561 cgttaaaaaa caccaaaaac cttctcaagc tgctcctcaa ggtggaattg ttgagatgga
    28621 agcgccaatt catgtttcta acgtgatggt ggttgactct actggtgtag ctggccgagt
    28681 tggctacaaa gaagtagacg gcaaaaaagt ccgtgtttct aaaaaaaccg gtgaagtttt
    28741 agataaataa aagtttaagg aaggagggtc ttactgaatg aaccgcctaa aagaaaaata
    28801 tcttaaagaa gtaactccat cattgatgga aaaatttaac tatagctctg ttatgcaaac
    28861 acctaaagtt gaaaaaatcg tcatcaacat gggtgttggt gatgcggtat caaacgctaa
    28921 aaacttagat aaagcagttg aagaattaac tttgatcact ggtcaaaaac caatgattac
    28981 aaaagctaaa aaatcaatcg ctggattccg tttacgtgaa ggaatgccaa ttggtgcaaa
    29041 agttacattg cgcggagaaa gaatgtacga attcttagac aaattggttt ctgtttcact
    29101 accacgtgta cgtgacttcc atggtgtaag taaaaaagcc tttgacggtc gtggaaacta
    29161 tactttaggt attaaagaac aattgatctt cccagaagta gattatgatt tagtagataa
    29221 agtacgtggt atggacatcg ttattgtaac aacagcaaac tctgatgaag aatctcgtga
    29281 gttgttgaca caattaggca tgccattcca aaaataatta aaggaggcga actacgtggc
    29341 taaaaaatca atgattgcta aaaacaaacg tcctgcaaaa ttttcaacac aagcttatac
    29401 tcgttgcgaa cgttgcggac gtccacattc agtttatcgt aagttccatc tttgccgtat
    29461 ttgcttccgc gaacttgcct ataaaggtca aattcccggc gtgaagaaag ctagctggta
    29521 aaaagtaaat tcgcataaga aggaggtaaa agatcaatgg tcatgacaga tccaattgca
    29581 gattttctaa ctcgtatccg taacgcaaac atggttaaac atgagtcttt agaagtacct
    29641 gcatcaaaaa ttaaacgtga catcgctgaa atcttgaaac gtgaaggttt cgttcgtgat
    29701 gttgaataca tcgaagatga caaacaaggc gtgatccgtg ttttcctaaa atttggtaaa
    29761 aacggcgaac gtgttatcac aaacttaaaa cgtatctcta aaccaggttt gcgtgcttat
    29821 gtcaaagctg acgaagtacc aaaagtattg aacggtttag gtatcgcaat catttcgact
    29881 tctgaaggtg ttgtcactga taaagaagct cgtgaaaaaa atatcggcgg cgaagttatc
    29941 gcttacgtat ggtaatcaag tagaatctac aaggaggtgt ctttaagtga gccgtatcgg
    30001 aaataaagtc gttgttatcc ctgaaggagt aacagttact caagacggaa acaacgttac
    30061 agttaaagga ccaaaaggtg aattgactcg tactttttca gcagatatca aaatgaatat
    30121 cgaaggaaac gaagtaactt tcactcgtcc aaacgacagt aaagaaatga aaacaattca
    30181 tggaacaact cgtgcgaact tcaacaacat ggttgtaggt gtcagcgaag gtttccaaaa
    30241 agcgttagaa ctaatcgggg ttgggtaccg tgcacaatta caaggatcaa aacttgtatt
    30301 gaacgttggt tactcacatc cagtagaaat cacaccacca gctggagtaa ctgttgaagt
    30361 accagctaac acacaagtaa ttgttaaagg tgcgaacaaa gaagaagttg gcgaattagc
    30421 tgcaaacatt cgtggaactc gtcctccaga accttataaa ggtaaaggta ttcgttatgt
    30481 tggcgaatat gtacgccgta aagaaggtaa aactggtaaa taatcgttta gaaaaattgc
    30541 atagaagcca gcagcaggaa aaaggcattt ggctgaagtt taccttcaaa aaaatacatt
    30601 tttacttgtg ccgcagcttc tatttttcga aacgtattta ctgcttgaaa tagtagtaca
    30661 ataaaaataa agaggtgaca attgtgatta caaaaccaga taagaacaaa acacgtcaaa
    30721 agagacatcg tcgtgtacgt aacaatatct ctggtactgc tgagcgccca cgcttgaacg
    30781 tttttcgttc taacaaaaac atctacgctc aaattattga tgacgtagcg ggtgtaacgc
    30841 tagcaagtgc ctctaccttg gataaagaaa tttcaggtgg aacaaaaaca gaagcagcac
    30901 aagctgtcgg taaactagtt gctgaacgtg cagctgaaaa aggcattaaa gtagtagtct
    30961 ttgaccgtgg tggatacctt taccatggcc gtgtgcaagc tttagctgaa gctgctcgcg
    31021 aaaatggact agaattttag aagaaggagg aataccattc atggtttata tcgatccaaa
    31081 acatttggaa ttagaagacc gcgttgttgc aattaaccgt gtaacaaaag ttgttaaagg
    31141 tggacgtcgt ctacgttttg ctgctttagt tgttgtcggt gacaaaaacg gacacgtagg
    31201 gtttggtaca ggtaaagctc aagaagttcc tgaagcaatc cgtaaagcaa ttgaagatgc
    31261 gaagaaaaac ttagttgaag tacctatggt tggttcaaca atcccacacg aagtaatcgg
    31321 tgcttttagt ggtggtagaa tcctaatgaa acctgctgta gaaggttctg gggttgctgc
    31381 tggtggacca gttcgtgccg tattagaatt agctggggta gcagatatca cttctaaatc
    31441 tttaggctca aatacaccaa tcaacgttgt tcgcgcaaca gttgaaggtt tgaaacaatt
    31501 gaaacgtgca gaagaagttg cagaacttcg cggcaaatca gttgaagaat taatcggata
    31561 aggaggacaa caataatggc tgaattaaaa gttactttaa aacgcagtat tatcggacgt
    31621 cctcaaaacc aacgcgatac agttaaagcg ttaggtctta caaagattaa cagttcagtg
    31681 gttaagcctt ctaatgaagc aattaaaggc atgatcaata ctgtttctca cctagtggac
    31741 gttgaagaaa tttaatttag aaaaatcata actagaacat taaggaggtg ccaaacctat
    31801 gaaacttcat gaattaaaac ctgctgaagg ttcacgccaa gtacgtaacc gtgttggtcg
    31861 tggtacttca tctggtaacg gaaaaactgc cggacgcgga caaaaaggac aaaaagctcg
    31921 ttcaggcggc ggtgtgcgtt taggatttga agggggacaa acaccattgt tccgtcgttt
    31981 accaaaacga ggattcacaa acgtgaaccg taaagactat gcagtcatta atcttgatgt
    32041 attgaaccgc tttgaagacg gagctgaagt aactcctgtt gctttagttg aagctggaat
    32101 cgtgaaaaac gaaaaagctg gaatcaaagt attagctaat ggggaactaa acaagaaatt
    32161 aactgtgaaa gcagcaaaat tctctaaagc agctcaagaa gcaattgaag cggctggtgg
    32221 atcaatcgag gtgatctaat gtttagacta ttaaaagatg cttttaaagt aaaagacatt
    32281 agatcgaaaa tcctatttac tgtatttgtg ttgtttgtat ttcgcctagg tgcacatatt
    32341 actgtaccag gcgtaaatgc tgcttcattg caaaaccttt cttctttgcc atttttcaac
    32401 atgttgaact tggtaagtgg tagtgcgatg caaaactttt ccattttctc aatgggggtt
    32461 tcaccataca ttactgcttc tatcgtcatt cagcttcttc aaatggacat cgtgcctcga
    32521 tttgttgaat ggtcgaaaca aggggaagtt ggtcgtaaaa aactgaatca agcgactcga
    32581 tatctgacat tagtattggc atttatccaa tctgtcggta tcactgctgg attcaatcaa
    32641 tacgctcaat taggtttcgt gaataatcct aacgtagcaa cttacgtaac gatcggtttg
    32701 attctaacag ccggtacgat gtttgttact tggctaggag aacagatcac agaaaaaggt
    32761 atcggaaatg gcgtatcaat gatcattttt gcaggtatca tttctagatt gcctgaaagt
    32821 atcagagaaa tttatgaaga ttactttata aatattgatc cttcagacat atggaaatct
    32881 gctatttttg tggccatttt actagtagcg gtcttgttga tcgtgacatt cgtaacattt
    32941 ttccaacaag ctgaacgcaa gatcccgatc caatatacga aacgcgtagc tggcgcgcct
    33001 acaagcagct atctgccgtt gaaagtaaat gcagctggag ttattccagt tatctttgct
    33061 agttcgttta ttgctacgcc aaatgctgtt ttacaagcat tgagcggtag attcggttct
    33121 gaaaactggt acaaagtgat gatggagatc tttaactata atacagcacc tggtgctacg
    33181 atttatacga ttctgatcgt tgccttcact ttcttctacg cctttgttca agtaaaccct
    33241 gagaaattag cggaaaactt gcaaaagcaa ggaagttata taccaagcgt acgccctggt
    33301 aaggggactg aagagtatgt ctcaagatta ctgatgcgtt taagtacagt tggtgcagtc
    33361 ttccttggtt tagtggcatt gcttccaatc attgctcaaa tggtttggga tcttccacaa
    33421 tcaatcggat taggcggtac aagcttactg atcgttatcg gtgtagcttt agaaactgcg
    33481 aaacagttag aaggcttaat gctgaaacgt aaatatgttg gattcattga gtaagaaagc
    33541 gtgttgaggt ttcccctcaa cacacgttct cacacattta ttaggaggga aaacaatgaa
    33601 cctcatttta atggggctgc caggcgcagg taaaggaaca caagctgaaa aaatcattga
    33661 tgcttatggt attccgcaca tctcaacagg agatatgttc cgcgcagcga tgcaaaacga
    33721 aacagctctt ggcctggaag caaagtctta tatggataaa ggtgcattag ttcctgatga
    33781 agtaacaaat ggaatcgtga aagagcgact agctgaacca gatacagaga aaggcttctt
    33841 attagatggc tttccacgta cgctagatca agcaaaagct ctggacgcaa tgctaaaaga
    33901 tttgaataaa aaaattgatg ctgtcatcga tatccatgtt ggtgaagaaa tcttggtcga
    33961 acgtctagcc ggccgattta tctgcagcaa ttgtggagca acttaccata aaattttcaa
    34021 ccctacaaaa gttgaagaca catgtgatcg ttgcggtggg catgaattct atcaaagaga
    34081 agatgataaa cctgagacgg ttaaaaatcg tctggctatc aacatcaaaa atagtgaacc
    34141 aatcttggct tattataaag aacaaggttt gctaaacact attgatggtg ctcgtgaaat
    34201 cgatgctgta tttgcagatg tacaaaaaat cattgaaaaa tagcacgatt gcccgtagcc
    34261 gacttgcatt atgcagttac tcttgtcaag ttcaataaat tatgatagaa tatgtcagtc
    34321 cgacttctag agaatcctct agatgatcca ttttgagaat tttgaggtgg gaactgcagt
    34381 ttccgccatt ttatcgtgtg aaaatgcgaa acaagtacaa ggaggtactg tgcgtggcaa
    34441 aagaagatat gattgaagtc gaaggtacag tcgtcgaaac tttgccgaat gcaatgttta
    34501 aagttgaact agaaaacgga caccaagttc ttgctactgt ttcaggtaaa attcgtatgc
    34561 attacatccg tatcttacct ggtgacaaag taacggttga attgtctcca tacgatctta
    34621 accgtggccg tattacttat cgctttaaat aattgtactc cgtaaaacta caggaggtat
    34681 tatcatgaaa gtaagaccat cagtaaaacc aatgtgcgaa cattgtaaag taattcgtcg
    34741 taaaggacgc gttatggtga tttgcccagc aaatccaaaa cataaacaac gtcaaggata
    34801 attggaggtg ctataaatat ggctcgtatt gcaggagtag atattcctcg tgataaacgt
    34861 gtagtagttt cacttactta tatctatggt attggtaaca ctactgctaa aaagatctta
    34921 gctgatgctg gtgtatctga agatgttcgc gttcgtgaat taacgaatga acaaacagat
    34981 gctatccgtg cagaagtaga taaattaaaa gttgaagggg atcttcgtcg tgaagtgaac
    35041 ttgaacatca aacgtttgat ggaaatcggt tcttaccgtg gaatccgtca tcgccgtgga
    35101 ttgcctgttc gtggacaaaa cacgaaaaac aatgcacgta ctcgtaaagg cccagctcgc
    35161 gctatcgctg gcaagaaaaa ataattctta agtgaaggag gttaaaactt catggcagca
    35221 aaaaaagtga atcgtaaacg ccgtgtgaag aagaatatag aagcaggtat tgcacatatc
    35281 cattctacat tcaataacac tatcatcatg atcactgata ctcacggaaa tgcattagcg
    35341 tggtcatcag ctggttcatt aggtttcaaa ggaagcaaaa aatcaactcc ttttgccgct
    35401 caaatggcag cagaaactgc tacaaaagca gcaatggaac acggattaaa gactgttgaa
    35461 gtaacagtta aaggacctgg ttctggacgt gaagcagcaa ttcgttcatt acaagctaca
    35521 ggtttagaag tgactgcaat tcgtgacgtg actccagttc ctcataatgg tgcccgccct
    35581 ccaaaacgcc gtcgtgttta atgagtgccg ctcattttga gttttatcag taacgtcatt
    35641 gaacaagaca cttttcgttt tgaaaggggt aaagataaga atgattgagt tcgaaaaacc
    35701 aagaatcgca aaaattgatg aagaaaaaga ttatggcaag ttcatcgttg aacctcttga
    35761 aagagggtat gggactactt tagggaattc cctacgtcgt attttattat cttctttgcc
    35821 aggtgctgcc atcactaata ttcaaatcga tggtgtactg catgaattct ctaccatcaa
    35881 aggtgttaga gaagacgtca ctcaaatcat cttgaacatt aaaggcttag cgctgaaaat
    35941 gtatggccaa gaagaaaaaa cccttgaaat cgatattacc ggacctgcta cagtaactgc
    36001 tggcgatatt atcgttgata gtgatgtaga aattcttaac aaagatatgt acatctgtag
    36061 tgtctctgaa ggagctacgt tccatgctcg cttaacagtg aaacctggac gtggttatgt
    36121 tcaagcagat gaaaataaaa aagaagatat gccaattggc gttcttccag tcgattcaat
    36181 ctacacacct gttcgtcgtg tgaactatca agttgagaac acacgtgttg gacaccgtga
    36241 agattttgat aaattgacga tggagatttg gacagatggt tcaattgaac cgttggaagc
    36301 aatgagtttg tctgcgaaaa tcatgactga acatttggat atttttgtga acttaactga
    36361 tgaagcaaaa aatgctgaaa tcatggtcga aaaagaagaa acacaaaaag aaaaaatgtt
    36421 ggaaatgacg atcgaagagt tagacttgtc tgttcgctct tacaattgtt tgaaacgtgc
    36481 aggaatcaat actgtacaag aacttacaaa taaatctgag cctgaaatga tcaaagtccg
    36541 taacttagga cgtaaatcat tagaagaagt taaagcaaaa ttgcacgact taggtttagg
    36601 tttacgtaaa gaagattaaa accttttacg aaggagggaa accaacgtga gttatcgtaa
    36661 actaggacgc acatctagcc aacgtaaagc gatgttgcgt gatttaacaa ctgatttatt
    36721 aatcaacgaa cgtattgtga ctactgaagc tcgtgcgaaa gaagttcgct caactgcaga
    36781 aaaaatgatc actttaggta aacgcggaga tttgcatgca cgtcgtcaag ctgcggcttt
    36841 cgtacgtaat gaagttgcca gcgtgcgtga agaaaatgag gacatcgtca ttgaatcagc
    36901 tttacaaaaa ctgttcaacg atattgcacc tcgttacgca gaacgtcaag gcggatacac
    36961 acgcattttg aagacagaac caagacgcgg tgatggtgca ccaatggttg tcttagaact
    37021 tgtctaaaat cacactaata acatcacttt atagatgatt cttttagagt gttatgatgt
    37081 tggaagatat ttttccgagt ctagctcagc gctgctcctc aagcgaatat cgcttgggga
    37141 gtaagcactc atcataaagt gtttttttat accttcaatt acctgtcatg acagatatgt
    37201 ttgaaggcat aaaaaaacat tttaagcgag aaataattga aaattttgtc tttttttgat
    37261 agtgttagta ggaactttag gatgaagaag gagtaatcat ggagccaatt attgaattag
    37321 aaaagataaa ttataaatac cagccagatg atcttcgccc cgcattgaaa gatgtctcct
    37381 ttacaattga taaaggtgag tggatcgcta ttattggaca caatggttca ggaaaatcga
    37441 cacttgccaa aacgatcaat ggattgcttc tgccagaatc agggatcgtg aaagtgggaa
    37501 atcaaatatt ggatgaagaa aatatttgga cgatccgaca gatggttgga atgatttttc
    37561 aaaatccaga caatcagttt gttggttcga cagtagaaga tgacgttgct tttggtcttg
    37621 aaaatcaagg gattcccaga gaagaaatgc tagtgcgtgt gaaagatgca cttgagaaag
    37681 ttagaatggc tgagtttgct tcacgcgagc ctgctcgttt atcaggaggg caaaaacaac
    37741 gtgttgccat tgccggcgtg gttgccttac gacctgatat cattatttta gatgaagcaa
    37801 ccagtatgct agatccagaa ggtcgtgaag aagtgatctc tacgatcaag aagatcaaag
    37861 aagaaagtca attgaccgtt atatctatca ctcatgatat tgatgaagca gctaatgcca
    37921 atcggatact agtgatgaga caaggcgaac ttgtccgtga aggtacacca aaagaaattt
    37981 tttctgctgg tcctgaactg atcgacttag gtctagatct accatttcct gaaaaactga
    38041 agagcgcact gaaagaacgt ggggttgatg tgcccagtga atatatgaca gaagaaagga
    38101 tggtggattg gttatggaca tccgttttga acaagtagac ttcacttatc agccgaatac
    38161 accatttgaa caaagagcct tatttgacat caatatgacg atcaaagaaa acagttatac
    38221 tgcattagta gggcataccg gaagcgggaa atcaacctta cttcagcatt tgaatgcgct
    38281 tgttaagcca acaagcggta cagtccatat tggagaacga gatattcagc cggatacgga
    38341 taataagaat ttaaagccca tccgaaaaaa agttggcatc gtctttcagt ttccagaagc
    38401 acagctattc gaagaaacgg tagcaaaaga cattgctttc ggtcctaaaa actttggtgt
    38461 cagcgaagaa gaagcactag tcctagcaaa agaaacatta gaacaagttg ggctggatga
    38521 aagctatttg gaacgttcgc catttgaact ttcaggaggg caaatgcgta gagtagcaat
    38581 cgctggtgtg cttgccatga ggccggaagt acttgtattg gatgaaccaa cggcaggact
    38641 tgatccccag ggcagaaaag aaatgatgga gatgttttgg cgtcttcata aggaacagca
    38701 gatcactatt gtattagtta cgcatttaat ggatgatgta gccaattttg cagattatgt
    38761 ctatgtgttg gaaaagggac gaatcgttaa tagcggggag ccgcaagagg tctttcaaaa
    38821 cattgaatgg ctaaaagaaa aacaactagg cgtacctact gccaccgagt ttgcagaaga
    38881 attaatggct aaagggatga actttgctac cttgcctttg acagctgaag aattagcaga
    38941 cgctatcgtc cattatgctg gaggaaatgc ccatgatgaa taaattgata tttggtcgtt
    39001 atatgcctgg agattccttg atccatcgat tggatccccg agctaaatta ttagccagtt
    39061 tttattttat cggaatcatt tttttagcaa ataattggga aacgtacctt ttgttggcat
    39121 ttttcacatt actttctgtc ttcttgtcaa aagtagattt gaaatttttt atccgaggca
    39181 ttcgcccatt gatctggcta atcctcttca ctgtagcttt gcaggtcttg tttacaagtg
    39241 gcggagaaac ttattggagt tggggtgttt tccatatcac tgaatttggt gtgatgaacg
    39301 gcctctttat tttctgtcga ttcgtgttga tcatttttat gtcgactcta ctcacgctta
    39361 ctacaccgcc tttggaatta tcggatgcga ttgagtactt gcttcgtcca ttgaaagccg
    39421 tacggttccc agtccacgaa atttcattga tgctgtcgat tgctcttcgt tttgtgccta
    39481 cattgatgga cgaaacagaa aagatcatga acgctcagcg cgctcgcggt gttgattttg
    39541 gtgaaggaaa cctagttcag aaaatgaaag cagtcattcc tttgctgatt cctttatttg
    39601 tcagcagctt caaccgtgcg gaagatcttg cgactgctat ggaagcaaga ggctacaaag
    39661 gtggggaagg aagaacgaaa tatcgtgtat tgcattggca taatagagac acgatggtta
    39721 tcattgcttt tgtattatta actgtgctac tgatcttttt aagaggataa acgttcagtg
    39781 ggccgctatc tattcttttc ttgctgatct gtttgcaaga aaggatgcaa gcagtgtatg
    39841 acaagaaata ttagggtctg tggcagacta aggtcacagg cctctttctt ttatctgaaa
    39901 agaaagaacg aaaaaatttt atagaaaaat cattattcca gataaccttt taggaacaaa
    39961 ggagagaaaa acatggtacg ttacaaagca atcattgcgt acgacggaac aaattttaat
    40021 ggattccaaa aacagccaaa tgggcgtact gttcaagaag aagtagaaaa aacattacaa
    40081 aaaatggcaa atggaaaaga aatcacggtc tttggttcgg gaaggacaga tgccggagta
    40141 catgcgatcg ggcaagtaat ccattttgat tatccagagg agcgtccgtt agaacgaatg
    40201 cgtttcgcgc tagataccca gtcaccagag gatattgctg taagacaagt agaaattgtt
    40261 tcagaagact tccacgcaag atatttggtc aaagaaaaga cgtatcaatt tcgagtggat
    40321 atagggaaac cacgcagtcc ttttcgacgc cattatgcaa gttattttcc ttacccactt
    40381 gatttggaaa aaattcagcg ggcgcttcct gatttattag ggactcacga tttcacctct
    40441 ttttgtgcat caggaagctc gatagaggat aaaatacgta cgatctacga ggctaagatg
    40501 gaggtaaatg aaacaaaaga cgaattgttg tttacattta ggggaaatgg cttcttgtat
    40561 aagatgatac ggatcttagt aggaacgctt ttgaaaattg gcaatggccg attgcctgaa
    40621 gatagtatcc cagatattat tgcgaaaaaa gatcgtaatg cagcaggacc tacagcacac
    40681 cctgaaggac tttatttgta tgaagtgaaa tatgaataag aggttatgat agaagtggtc
    40741 agcttcaaga aataagaagg tatttacgaa aattacttct caaaattttg caaaatttcg
    40801 gcttattttc gaagaagcta cttctggaac accgtttaag aaagaggtcg tgagcctgtc
    40861 atttaactct gagtcacaaa gagcaatttc aaaaaacagc ttctcatctt tgatgaatat
    40921 cgtgacttgt ggacgaggcg ttgataagtg aatcatcttt ttgttaccta ttttttctgt
    40981 aaccaaaaaa cgatggaacg ttgttttgat tgcccttggt gcggacagtc tgaacaggtt
    41041 ccatcgtttc tttattttct acgtcttctt ctgtgtgtgt ttcgtggttg ttcttctatt
    41101 gctggatcaa tcgtgccata aaaggggcga ttgcggtcaa ggtaccagac ataaagaata
    41161 aacaatgcaa tacataggac aaaaaggatt gcacctatcg taaatccttc aggcaagtaa
    41221 ctgaaagtga cagtatgttt acctgctgga acttttatca tcaagaatgc gtctttgaaa
    41281 ttgctgacct tgactggttt cccatcaatt ttagctttcc atcctttgtc ataaggaata
    41341 gttgtcacca acatggaatc ttcagcggta tttacagtcc cagaagcttt tctgccagtg
    41401 gcttccattt cgacaccttt ttctttgatc tgattcattg cggcttgata ggctacaaca
    41461 tctagaccaa cgactttagg ctccatgaaa cttatttctt tcgtcccata aaaacttgca
    41521 gtaaatgtca ctgttgtctc ttgaggataa tagccaagat catagtattg accagaaatg
    41581 ttgatttgcg tttttcgcga tgtaccattg acagtgatcg tcgcactaga actttctaat
    41641 tgcgcaaaat tggttgggaa caaacttaag taagcttgtg tatttgcagg cacatggact
    41701 tcccaagtga gctcttttgg ctggttatct gctgtttctg agtaagtgac gccagtcgtt
    41761 gtctttgtga tcgtcgtatt caactgattc ttcaaagtag gtgtatagaa tgtaaaatac
    41821 tgttcattta gaccagacaa tgtattgatc aagtttgtct gactggtcaa attatctgtc
    41881 actggctgct caacatcatt aacggacaac ggtgcagtaa atgctagtgg caatgcattg
    41941 acattctgat acagtgtata gtcgccgctt tgaccagctt ggcggaagcc atatttactg
    42001 aacgtgcttt cgtttttaga aatgttatat ttgattcctg taaacccatc catcagcaaa
    42061 gtattattgt tataacggat attcaaattt gtccctcttg aacggaaacc taatttatcc
    42121 aggtaagagg aagaattacg atttcttata gaagaaaaca aactgatacc actgtaacca
    42181 tagttgatcg aatcgtttgc agatactgga tccagatttt ccaagcggta aaacgagtcg
    42241 gaatcttttt ttgtttcatt tacaagagct ttcaagtcag gataaggatc agtatataaa
    42301 ctgcgggaag cataattcca atcatcaaga atgccatgga tcatactgtt cgtattgacc
    42361 agtgcttcag cagacatgat caataataac aatatactca gatgattgaa tgaaagtttt
    42421 ttatgctgat aaaatcccac agcaaaagca taaatcccta agaaaaccac tgtcaataca
    42481 aaggaagtca tcgtcacata ggtgtagctt ccttcaggtt tgatgcccca tgccaccgca
    42541 aagagagcag ctacgaccaa aatgattcct aacaacagcc caccatcgtc ttgttctatc
    42601 ttttcccatc cttttcctgc aagcaggagc actaagaaag agaacagata ggcataacgg
    42661 aataaaaaca tattcggtgc atgcatacca tgccagaaca aattcaatgg aacaagataa
    42721 aaacttgcga taagtatggc gaaaagactg ccgtacaaaa atttattttt caacggtact
    42781 ttccgactgg caaaataaaa cagacagagg actaatggta gcagacctgc gtaaacgaac
    42841 ggaatcgatc cgtattttgt cgtgtcataa acaccaatca tatttttcat gattagatcc
    42901 agataagaag tcgcttctgt tttcaacgta gtgatctgac tcagtgcctc gccatttgac
    42961 cgaagatcca gcactgctgg caatacaatg atcattgaag cgccgcctgc cagtaaagaa
    43021 gtgataccat aaggaacaat cgttttttta tattctgacc agtttgtcaa tagacgggcg
    43081 ataaaatata agaaagaaaa tacgccaatc ataaaaccca tataaaaact ggataaaaac
    43141 aagagtaaat aactgataaa taaaagtttc ggccgtttaa gatccataac acgatgaatc
    43201 cccaaaatca ctaatggaag ataaacaaat gcatccagcc acatgatgat ctccgaatgt
    43261 gcagtgataa atgagatcaa tgcgtaactg acgctaagtg caacatgccc ccagcgagga
    43321 atcttgaacg tacgtgcaag aaaccaaaaa gccaatccgg cacagccgac cttaagcaaa
    43381 gtcagaacat acagtgcatc tggcatcatc tggtttggaa aaaagaacac cagtggcgtg
    43441 aagatcccgc ctagatagta agaaattaaa gataaataat tcaatcctag agaagcattc
    43501 catgtataaa aaatactttg ctttccaaga agaacatttc ggaagctggc atgaaaatta
    43561 gaaaactgtg aaaaggcatc acttgccaaa atacttctgc tgcttccagg atagattccg
    43621 atacttagat aaacaagtgc taaaatcaaa aacggaatca ggaaactagc tgctgcgtag
    43681 cgtccgtttt ctctaaagaa tgactttaaa ttttttttca tatttatttt ccaccaaatt
    43741 tctatgaagt tgtattcagt ttatcatatc cttgtttttc tctgcttaaa aagagtctag
    43801 ttttcacaaa aaaataaaat ttagaataaa taacttcata atttgttcgt ataacaaaaa
    43861 taaacgcaaa aaaagggtag gaatcaaaag tcccaccctt aaaaattttt attaaataaa
    43921 aaaccgttca ctatctgaac ggtccaagta tcattcaatt aaacgcgttc aacttttcct
    43981 gatttcaaag cacgagttga cacccaaact tttttaggtt ttccgtcgac cataacgcga
    44041 actttttgta agttaggttt aacagtacgt tttgttgagt tcatagcgtg tgaacggttg
    44101 tttccacttt ttgtcttgcg acctgtaaag taacaaactt ttgccatggt aatttcctcc
    44161 tttgccttga tttggagcaa tagaataatt cagctcatac cagactaaca tatcatatag
    44221 aatgggaatt tgcaagtaca ttttcataga tacaattgac aattgagaag aataattgta
    44281 aattaaacaa ggttatacct gaaattttgc aaggtttttg agtactttgg gtaactgcct
    44341 tttatttcaa gttgcgctat gataaaatat gatagaagaa aaatgggagt tttttacccg
    44401 ttaaggaggc tcattcatgg ctgtaaaaat tcaaacgtca gctggaacca ttgaaattac
    44461 caatgatgta attgcaacag ttgttggtgg cgccgcgaca gatgtgttcg gtgttgtagg
    44521 aatggccagc aaaaatcaaa ttaaagataa tattactgag attttgcgta aagaaaatta
    44581 ctcaagagga gtagttgtcc gccaagaaga taatggtatt gctgtagatg tttatacgat
    44641 tatcagctat ggtacgaaaa tctctgaagt ttcccgcaac gtccaagaaa aagtgaaata
    44701 caatctagag gcattattag gcgtaactgc caattcagtt aatgtctttg tacaaggtgt
    44761 tcgtgtactg ccagactagg caggagactg gctggtaact cagcagtcga tctgaaggag
    44821 gattatctag gtgagtgtaa cagaaataag tgcaagccag ttccaggaaa tggtccaggc
    44881 aggtgcgaat cgtctgcaag taaatgcaga atacgtcaat tcattaaatg tattccctgt
    44941 gccagatggc gatacgggaa caaatatgaa tctatctatg actagtggag ctaaggcagt
    45001 agtagattca acctcagaaa aagtaggaga attagcagca atgctttcaa aaggattgct
    45061 gatgggtgcg cgcggaaatt ccggcgttat tttgtctcaa ttgtttcgtg gattttctaa
    45121 gcaaattcca gatgttgtct cattaaatgc ttctgatctt gctgcagcct tcactcatgg
    45181 agttgaaact gcgtataaag cagtaatgaa accagtagaa ggaacgattt tgacagtagc
    45241 tagagaagca gcgaaagctg gagaaaagaa agcaaaaagt actgatgatg tcgttgaagt
    45301 aatgacagct gttgtaaaag gagcaaagcg tgctttagct aaaactccag acttgcttcc
    45361 agtcctaaaa gaagtcggtg tcgtagacag tggagggcaa ggactgctgt ttgtttatga
    45421 gggcttttta agtgcattga atggagaatt ccaagcggat gaaacatatg aaccttctcc
    45481 agcagaaatg gatgatatgg tcaatgcaga acatcatcgc agtgtccaag gtcaaatggc
    45541 gacagaagat atccattttg gttactgtac agaaatcatg gtaaaaatcg gagaaggacc
    45601 tacagtcgat agtacattcg attacgaaga attccgtaac tatttagatg gtataggaga
    45661 ttctttactt gtggtcaatg atgacgagat catcaaagtc cacgtgcata cagaaaaccc
    45721 aggagaagtc ttgaattatg gacaaaaatt cggttcatta gtcaaaatca aagtcgacaa
    45781 catgcgcttg cagcatgaga cgattttgga acacgatgaa gaagtgaacg agtttgcgaa
    45841 aaaagaaata ccacgtatcc cgtatggtat cgtagcgatc gctgctggta aaggcgtcca
    45901 agagctgttt gaaagtttag gcgctaatta tgttatcagt ggtggacaaa caatgaatcc
    45961 aagtacggaa gacattgtta aagcgattca agaagtaaac gcagataaag tgattgtctt
    46021 gccaaacaat aagaacatct tcatggcagc tgatcaagca gcagaagtag cagatatccc
    46081 agtagcagtt gttccttcaa gaacgatttc ccaaggattg acagctatgc tttcattcaa
    46141 tgatcaagct tctttagaag aaaacaaaga aacgatgaca gaaatgttgt ctagcgttgt
    46201 gagcggtcaa gtcactcacg cgatcagaga tacctcaatt gacggtgtag agatcaaaga
    46261 aggagacttt ttgggcatga tcgatggaaa aatcctgatc tctaacccag atatcacagc
    46321 aacagcgtta agtacactag aacaaatgat caatgaggac acagagatcg taacgatctt
    46381 ggcaggtgat gaaggaacag ttgaacaagc aaatgaattt gctgagaaac taacagaaaa
    46441 acatcctgat ttggaaatcg agatccacca aggagatcag cctgtctatc cttatctatt
    46501 ttctgctgaa taatttctgt gtcagaatat ctttctaaca cagtattcat tcataaaagg
    46561 ccttcggtta gtgatcaggt atgatcgctg accaagtcct ttttttgtta aaggagggag
    46621 gaaaaattta tgcgttcttt agatgatcca atcactatgc tggcaggagt cggagaaaaa
    46681 agagcagcta gtcttgccag cttgggcatc caaacgattg aagacttact gacttattat
    46741 ccttttcgct acgaagatat tcaggaaaga aatctaaacg agatccagga tcaagaaaag
    46801 gttactttga aaggaatcgt agtctcgcct cctgtgatga atcgatttgg ttataaaaaa
    46861 agccgattgc aatttcgtat gatgcaagaa catgatgtgt tcaacgtttc tttttttaat
    46921 caaccttatc taaaagaaaa agtcatttta tcggaggaaa tcgccgtata cggtaaatgg
    46981 gatgcaaagc gtaaagcctt gaacgggatg aagattttag gttctcaaac aatggatgac
    47041 ttttcgccga tatatcacgt caacaaatca attcgtcaaa cgacattagt tgatctaatc
    47101 aaaaaagggt ttcaagaata cggcacattg attgaagaaa atctgccaaa caaccttgtg
    47161 gagaaatatc gactgcttga ccgagcttct gctgtaagaa gtatgcattt tccaaaagac
    47221 cacgaagaaa accatcaagc caaacgacgt gtcgtttttg aagagttctt tttatttcaa
    47281 atgcgtcttc aaggattgaa aaaagcagaa aaagcagaaa cgaatggttt ggagatcctt
    47341 tatgatgtcc aaaaattgaa acaatttact caaaagctac cttttgaact gacaaatgca
    47401 caaaaacgtg taacgaatga aatctgtcgg gatcttcgca gtccgcagca tatgcagcga
    47461 cttttgcagg gagatgtagg tagcggaaaa acagtggttg ccgcgattgc gttatacgct
    47521 accgttacag caggtttcca aggtgcgttg atggtgccta cagaaatctt ggcacaacaa
    47581 catatggaaa gcttgcaaca gctgtttgat cccaatgaag taaccatcgc attgattacg
    47641 gggtcaacga aagcaaaaga acgacgtgag ctccttgaaa aactagagca aggggaaatt
    47701 aacgtcgtga tcggcacaca tgcattgatc caagatggag tcgaatttca gaatttaggt
    47761 ctagttatta cggatgagca gcaccgtttt ggcgtgaacc agcggaaagt acttcgtgaa
    47821 aaaggctggc gaccagatgt cttatttatg acagcgactc cgattcctag aacattagct
    47881 attacagcat atggtgagat ggatgtatct atcattgatg aacttccagc tggacgtatc
    47941 ccgatcgaaa cacgctgggt aagaacacct caattggaca gtgtactgga ttggacgaaa
    48001 aaagaactgg caagaggaca tcaaatgtat gtcatctgtc cgctgattga agaatcagaa
    48061 gcattagatg taaaaaatgc agtggaaatc tatgaaaaac ttcgtgaact atttgcacca
    48121 aaatatgaag ttggcttgtt acatggtaaa atgaagaatc aggaaaaaga agtaatcatg
    48181 gaagccttca aagacaacca gatgcagata ttagtttcga ctactgtaat tgaagtaggg
    48241 gtaaacgtgc cgaacgcgac cgtcatgctg atcatggatg cggatcgatt tggattggca
    48301 cagctgcacc aacttcgtgg acgtgttgga cgcggaagtg atgcttccta ctgtatctta
    48361 gttgctaatc ctaaaaatga actaggtgtg gaacggatga aaatcatgac agagaccacc
    48421 aatggttttg tactaagcga aaaagatctg gaattacgtg gtcctggtga agtatttggc
    48481 ttccgtcaat ctggtctgcc tcaatttgcg attgctgact tggtgacgga tggaaatgtt
    48541 ttggaggtag ccagagaaga ggctgcagcc ctttggcaaa taaaggattg gcagatgctt
    48601 cctgagtatc agcctttagc gcaaagcctc caaatagaaa atcaggataa tcgttatttc
    48661 gattagcaga ctgtgctata ctttatactg atgacggaaa aaggagtgga aacaatgaaa
    48721 attgccgtag atgcaatggg cggagatcac gcacctcaag caattgtaga aggcgtggct
    48781 ttagcgcagc aagattttcc agaagtagaa ttcttacttt acggaaaaga agcagaaatc
    48841 aagaaatact taacaaatga aaaaaacatc acgattatcc atacagacga aaaaatcaat
    48901 agtgatgatg agccagtaaa agcaattcga cgaaaaaaga ctgcatcaat ggtccttgct
    48961 gcacaagcag tgaaaaacgg tgaagcagat gctgtatttt cagcagggaa tacaggcgcg
    49021 ctactagcag cagggttgtt catcgtagga cgtatcaaaa atatcgaacg ccctggcttg
    49081 atgtctacct tgccagtagt agggaaagaa ggtgccggtt tcgatatgct ggatcttggc
    49141 gctaacgcag aaaacaaacc agaacatttg ttgcaatacg gtattttagg ttctttttat
    49201 gcacgtaaag tccgaggtat cggacgtcct cgtgtcgcac tcttaaacaa cggaacagaa
    49261 gcaacaaaag gtagtgaagt gacgaaaaaa gcctatgaac tccttcagaa tgaaacaagc
    49321 ttgaatttta ttggaaacgt agaagctcgt gagctactca atggtgtggc tgatgtagtg
    49381 gtcacagatg gatttacagg caacgctgtt ttgaaatcta ttgaaggaac agcaatgaat
    49441 atgatgaatc tattaaaatc tgctattcta aatgaaggga tcaaaggaaa aatgggcgct
    49501 atgcttttaa aagacggctt acgttctttg aaagcagaaa tggattattc caaacatggc
    49561 ggtgccgttt tgtttggctt gaaagcacca gtcattaaaa ctcatggagc aacaggcccc
    49621 gatgctgttc gctatacgat ccgccaaatc cacacaatgc ttgagacaga tgtagttgga
    49681 caattagtcg aacaatttga aaaaagcgaa gaatagtatt tggatatctg aagcgatccg
    49741 ttccattgaa aaaaaggtgg acattcctga aaaatgccag taaaattagc catggtgtag
    49801 gtaattgaca agtgtatgat tcttggaggt gaaaaagttg actcgcgaag aagtatttaa
    49861 caaagtagcg aaaattattt caaatcattt tgagatagat accgataaag tgacggatga
    49921 attgaacatc aaagatgatt taaaagctga ttcaattagt attatggaat tcgttttaga
    49981 gcttgaagat gaattcggta cggaaatttc agatgaagac gctgaacaaa ttgaaacagt
    50041 cggtggcgca gtagattata tcagcagcca tcttaaataa tttcaaataa aag
//
LOCUS       DARDHA010000005        48918 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000005 DARDHA010000000
VERSION     DARDHA010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.005"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            4..783
                     /locus_tag="SDA10_000227"
     CDS             4..783
                     /locus_tag="SDA10_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEG4239162.1"
                     /translation="MQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNRRLPRVAGHKEG
                     METVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFFDTFVPELIKEN
                     VKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEIVTAVQEIAEEV
                     AKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFLLWQIAYSELYF
                     TKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            780..1580
                     /locus_tag="SDA10_000228"
     CDS             780..1580
                     /locus_tag="SDA10_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEG4239163.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1741..3009
                     /gene="rseP"
                     /locus_tag="SDA10_000229"
     CDS             1741..3009
                     /gene="rseP"
                     /locus_tag="SDA10_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEG4239164.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3077..4786
                     /locus_tag="SDA10_000230"
     CDS             3077..4786
                     /locus_tag="SDA10_000230"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEG4239165.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            4998..9356
                     /locus_tag="SDA10_000231"
     CDS             4998..9356
                     /locus_tag="SDA10_000231"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEG4239166.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9501..9974
                     /gene="rimP"
                     /locus_tag="SDA10_000232"
     CDS             9501..9974
                     /gene="rimP"
                     /locus_tag="SDA10_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEG4239167.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            9997..11172
                     /gene="nusA"
                     /locus_tag="SDA10_000233"
     CDS             9997..11172
                     /gene="nusA"
                     /locus_tag="SDA10_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEG4239168.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11194..11487
                     /locus_tag="SDA10_000234"
     CDS             11194..11487
                     /locus_tag="SDA10_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEG4239169.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11484..11795
                     /locus_tag="SDA10_000235"
     CDS             11484..11795
                     /locus_tag="SDA10_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEG4239170.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11808..14114
                     /gene="infB"
                     /locus_tag="SDA10_000236"
     CDS             11808..14114
                     /gene="infB"
                     /locus_tag="SDA10_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEG4239171.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14141..14488
                     /gene="rbfA"
                     /locus_tag="SDA10_000237"
     CDS             14141..14488
                     /gene="rbfA"
                     /locus_tag="SDA10_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEG4239172.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14645..14818)
                     /locus_tag="SDA10_000238"
     CDS             complement(14645..14818)
                     /locus_tag="SDA10_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4239173.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            15266..17628
                     /locus_tag="SDA10_000239"
                     /pseudo
     CDS             15266..17628
                     /locus_tag="SDA10_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            17996..18918
                     /gene="truB"
                     /locus_tag="SDA10_000240"
                     /pseudo
     CDS             17996..18918
                     /gene="truB"
                     /locus_tag="SDA10_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
     gene            18922..19863
                     /gene="ribF"
                     /locus_tag="SDA10_000241"
     CDS             18922..19863
                     /gene="ribF"
                     /locus_tag="SDA10_000241"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEG4239174.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            20215..21000
                     /locus_tag="SDA10_000242"
     CDS             20215..21000
                     /locus_tag="SDA10_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG4239175.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            21013..21864
                     /locus_tag="SDA10_000243"
     CDS             21013..21864
                     /locus_tag="SDA10_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEG4239176.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(21988..22440)
                     /locus_tag="SDA10_000244"
     CDS             complement(21988..22440)
                     /locus_tag="SDA10_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4239177.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(22905..23273)
                     /locus_tag="SDA10_000245"
     CDS             complement(22905..23273)
                     /locus_tag="SDA10_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEG4239178.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            23423..23905
                     /locus_tag="SDA10_000246"
     CDS             23423..23905
                     /locus_tag="SDA10_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HEG4239179.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            23942..24952
                     /locus_tag="SDA10_000247"
     CDS             23942..24952
                     /locus_tag="SDA10_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEG4239180.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25067..25780)
                     /locus_tag="SDA10_000248"
     CDS             complement(25067..25780)
                     /locus_tag="SDA10_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239181.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(25970..26560)
                     /locus_tag="SDA10_000249"
     CDS             complement(25970..26560)
                     /locus_tag="SDA10_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEG4239182.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            27044..27271
                     /locus_tag="SDA10_000250"
     CDS             27044..27271
                     /locus_tag="SDA10_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEG4239183.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            27287..28453
                     /locus_tag="SDA10_000251"
     CDS             27287..28453
                     /locus_tag="SDA10_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEG4239184.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            28687..29730
                     /gene="hrcA"
                     /locus_tag="SDA10_000252"
     CDS             28687..29730
                     /gene="hrcA"
                     /locus_tag="SDA10_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEG4239185.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            29824..30387
                     /gene="grpE"
                     /locus_tag="SDA10_000253"
     CDS             29824..30387
                     /gene="grpE"
                     /locus_tag="SDA10_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEG4239186.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            30438..32267
                     /gene="dnaK"
                     /locus_tag="SDA10_000254"
     CDS             30438..32267
                     /gene="dnaK"
                     /locus_tag="SDA10_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEG4239187.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            32418..33584
                     /gene="dnaJ"
                     /locus_tag="SDA10_000255"
     CDS             32418..33584
                     /gene="dnaJ"
                     /locus_tag="SDA10_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEG4239188.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(33666..34331)
                     /locus_tag="SDA10_000256"
     CDS             complement(33666..34331)
                     /locus_tag="SDA10_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4239189.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            34307..34480
                     /locus_tag="SDA10_000257"
     CDS             34307..34480
                     /locus_tag="SDA10_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239190.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(34525..35262)
                     /locus_tag="SDA10_000258"
     CDS             complement(34525..35262)
                     /locus_tag="SDA10_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG4239191.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(35264..36766)
                     /locus_tag="SDA10_000259"
     CDS             complement(35264..36766)
                     /locus_tag="SDA10_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEG4239192.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            37025..39019
                     /gene="uvrB"
                     /locus_tag="SDA10_000260"
     CDS             37025..39019
                     /gene="uvrB"
                     /locus_tag="SDA10_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEG4239193.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            39029..41845
                     /gene="uvrA"
                     /locus_tag="SDA10_000261"
     CDS             39029..41845
                     /gene="uvrA"
                     /locus_tag="SDA10_000261"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEG4239194.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            42108..43373
                     /locus_tag="SDA10_000262"
     CDS             42108..43373
                     /locus_tag="SDA10_000262"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HEG4239195.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            43377..44108
                     /locus_tag="SDA10_000263"
     CDS             43377..44108
                     /locus_tag="SDA10_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HEG4239196.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            44243..45127
                     /gene="rapZ"
                     /locus_tag="SDA10_000264"
     CDS             44243..45127
                     /gene="rapZ"
                     /locus_tag="SDA10_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEG4239197.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            45124..46122
                     /locus_tag="SDA10_000265"
     CDS             45124..46122
                     /locus_tag="SDA10_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEG4239198.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            46156..47091
                     /gene="whiA"
                     /locus_tag="SDA10_000266"
     CDS             46156..47091
                     /gene="whiA"
                     /locus_tag="SDA10_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEG4239199.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            47634..48284
                     /locus_tag="SDA10_000267"
     CDS             47634..48284
                     /locus_tag="SDA10_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239200.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            48471..>48918
                     /locus_tag="SDA10_000268"
     CDS             48471..>48918
                     /locus_tag="SDA10_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HEG4239201.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNN"
BASE COUNT        16360 a         8399 c        10534 g        13625 t
ORIGIN      
        1 tatattcaag aaaaaagcga atttcaattt aataccgatg gaccaattcc tcagcacatt
       61 gccatcatca tggacggcaa tggtcgttgg gcgcaaaacc gtcgtttgcc acgagttgct
      121 ggccataaag aaggcatgga aacagtaaag aaagtcacaa aaaaagcatc aaggcttgga
      181 gtgaaagtgt tgacgcttta tgccttttcg actgaaaact ggaagcgacc aaaagatgaa
      241 gtaagttttc tcatgcaact acctgtcgac ttctttgata cgttcgtacc agaattgatt
      301 aaagaaaatg tcaaagttca tgtcatgggt tatgaaaacg tattgccaga acatacgcaa
      361 gatgctgtaa gacgtgcaat tgagcaaaca aaaaacaata ctggaatggt attgaatttt
      421 gctttgaatt atggaagcag agcagagatc gtgactgctg tccaagaaat tgccgaagaa
      481 gtagcaaaag gagaaataca cgcagaagaa atcgatgatg aattgattgc taagcattta
      541 atgacaggtt tcttgcctaa agagttacaa gatcctgagc tgatgatccg tacaagtgga
      601 gaagagcgaa ttagtaattt tctgttatgg cagatcgcat atagtgagct gtattttaca
      661 aaagcgcttt ggcctgattt tgatggtgcg catcttgaag aagcaatcgc ctcttatcaa
      721 aatcgagacc gtcgattcgg cggtgtaaaa aaaacagaag atgagaaagg ggatcagtca
      781 tgaaacaacg tgtggtcaca gcagccgtgg cgctagtctt gtttattccg attatatgga
      841 tcggaggcat agcggttgag cttacagcag caatcttagc agtaatagga gtttacgagc
      901 tgtttcggat gaaaggattg acactcttaa gttttgaagg agttttatcc gccgttggtg
      961 cagtgttact tgttttgcca aaagaaagat ggtttttctt tcttccagag aatgtaagta
     1021 catttatgtt attttatctt atagttatga ttcttttagg aacatcagtt atttctaaaa
     1081 atacgtatac gattgatgaa gcaggttttc ctgtgcttgt cagcttgtat gtgggaatag
     1141 gtttccagaa ttttgtcaat gcacgaacag aaagcttggc tgttttgata tttggtctat
     1201 ttattgtttg ggcaactgat atcggagcat atatggttgg tcgacgttat ggtcagcata
     1261 agttaagtcc tgatatttcg cccaacaaaa cgattgaagg tgcgctcggt ggtattggta
     1321 gtgctgttgt agtcgcattt ctttatttat tgctataccc agcaaaagaa ctatttaatc
     1381 atggtatgtt tgtcatgctt ctgatgacga ttattttttc agcagtcgga caatttggtg
     1441 atttggtcga atctgcaatc aaacgtcatt atgatgtgaa agactcggga aatatcttac
     1501 caggccatgg tggaatctta gaccgtttcg acagtttgct gtttgtattt cctatcatgc
     1561 atttatttgg cattatttag taagtatacc tgcgtttctt tgtgaatttc ataaaagacg
     1621 caggttttta tttttcatta tattgatact attggtgtct ttggtatact atggtaaaat
     1681 tatagtgttt tatcgttgtt ttataaaaaa cacaaaaaac aagtaaagga gaaaaaagct
     1741 atgaaaacga ttctgacatt tatcatcgtt tttggtatat tagtgattgt tcatgagttt
     1801 ggtcatttct tctttgcgaa acgatcagga atcctcgttc gcgaatttgc tatcggtatg
     1861 ggtccgaaaa tttatgggca tcaagcaaaa gacgggacaa catatacgct tcgcctatta
     1921 cctattggcg gttatgttcg aatggcaggc aatggggatg atgaaacaga aatggcacca
     1981 ggaatgcctc tttctctttt actaaattct gacggcatcg tagagaagat aaatttaagt
     2041 aagaaaatcc aattgacaaa tgctatcccg atggaattga gccgttatga tttagaagat
     2101 gagctgacta tcactggtta tgtgaatgga gacgaaacag aagtagtaac ctaccctgtt
     2161 gatcacgatg cgacgattat tgaaaacgac ggaacagaaa tcagaattgc gccaaaagac
     2221 gtacagttcc aatcggctaa attgtggcag cgtatgctga ccaactttgc tggacccatg
     2281 aataacttta ttttggcaat cctgttattc atcattctgg catttatgca aggtggtgtt
     2341 caagtgacga acactaatcg cgtaggagag atcatgccta atggagctgc ggcagaagca
     2401 ggcttaaaag aaaatgatga ggtagtcagt gtggacggaa aagaaatcca ttcatggaat
     2461 gatctgacaa cagtcattac gaaaaatccg gataaaacat tagactttaa gatcgaacga
     2521 gaaggtcagg tgcaatctgt cgacgtgaca ccgaaatctg tagagtcaaa tggcgaaaaa
     2581 gttggccaac ttggtataaa agccccaatg aatacaggtt ttatggataa gatcattgga
     2641 gggacgcgtc aagctttcag cggttcattg gaaattttca aagccttagg atcactattt
     2701 acaggtttca gtttagataa attaggcgga cctgtcatga tgtaccaatt gtcttcagaa
     2761 gcagcaaatc aaggtgtaac aactgttatt agtttgatgg cgcttctttc aatgaatctc
     2821 ggaatcgtca atctgcttcc gattcctgcc ttagatggcg ggaaattagt cttgaatatt
     2881 tttgaaggga ttcgcgggaa accattaagt caagaaaaag aaggaatatt gacattagct
     2941 ggctttggat ttttaatgtt gttgatggtg ctagttacat ggaacgatat tcaacgattc
     3001 tttttctaga aaaagttgac aaatagggta taataacaaa cgataactat tttgaggaaa
     3061 taaaggagta tatagaatga gacaatcaaa aatgttgatt ccaacattac gcgaagttcc
     3121 aaatgatgca gaagtattaa gccatcaaat cttgctaaga gctggatata tccgccaagt
     3181 atcagcgggt atttattcat atctgccatt agcaaatcgt gtattggaaa aactgaaacg
     3241 cattatgcgg gaagaatttg aaaaaatcgg agcggtagaa atgctgatgc ctgccatctt
     3301 gccagcagaa ctttgggaag aatccgggcg ttacgaaaca tatggaccaa acttgtatcg
     3361 tttcaaagac cgcaatgatc gaaaaatgat ccttggacca actcacgaag aaacttttac
     3421 tgatttgatg cgtaatgaaa tcaattcata taagaaatta cctttaaatc tttatcaaat
     3481 ccaacctaaa tatcgtgacg aaaaacgacc tcgcttcggt ttgcttcgcg gaagagagtt
     3541 tatcatgaaa gatgcgtatt ctttccatgc aacggaagaa agtttggacg agacatataa
     3601 agattacgaa aaagcctaca ccgaagtatt caagcgttgt ggcttagaat ttcgctcgat
     3661 tatcggagac ggtggcgcaa tgggcgggaa agactcaaaa gaattcatgg cgatctcaga
     3721 aatcggggag gatacgatct gttattcgac tgaaagtgat tatgctgcaa acttggagat
     3781 ggctacaagc tactatgtat caaaaaaatc gcatgaaaca cagcttgaat tggagaaagt
     3841 agctacacct aacgtaaaat caattgacga agtagcagat ttcttttctg ttgaacctca
     3901 aaaaatcatc aagtctgtat tgttcattgc ggatgaacaa cctgtccttg ttttggtacg
     3961 cggagaccat gaagtcaatg acgtaaaact gaaaaacttt ttatctgctg actttttaga
     4021 agaagcaaca gaagaagaag cacaaaaata tctaggtgct gagtttggtt ctgtcggacc
     4081 agtcggtgtt tcagatgacg taaaagtata tgcagaccgt tatgtccaag atttagcgaa
     4141 tgccattact ggagcgaacg aaacaggatt ccactataaa aatgtgaatc cagaacgcga
     4201 tttcactgta ctttcttatg aagatcttcg ttttgtacaa gaaggagatc cttcaccaga
     4261 caacaatggt gttcttgcct ttactcgagg aatcgaaatc ggacatatct tcaaaatcgg
     4321 cactcgttac agtgaatcta tgggagcaac tgtactagac gaaaatggac gagaaaaatt
     4381 tgtgattatg ggctgttatg gtatcggtgt aagtcgttta ttatcagcaa tcgtagaaca
     4441 acattcagat gaacaaggaa tcaactggcc aaaaggaatt gcgccatttg atttgcatat
     4501 cgtccaaatg aatctaaaag acgaatacca aacaaacctg acgaatgaac tagaagaagc
     4561 gatgacaaaa gctggctatg aagtactggt tgatgacaga aatgaacgcg caggagtgaa
     4621 attcgcggat tctgatttga ttggctgtcc aatcagaatc actgtcggta aaaaagcagt
     4681 ggatggaatc gtagaagtga aaatcaagaa aactggcgaa atggttgaag tacgtaaaga
     4741 agaattggtg aatactttac caatcctctt gaaccaaggc gaataataag cgttaaaaca
     4801 cagaaaataa aaatagctga atcagctgtt tttgacaatc tgatgattcg cttttaaaat
     4861 tggaactgcg atttgaatcg cagttccaat tttttaatgt aaaactcaga aaaaacgagt
     4921 atactaaaag gtactgaaaa agaagaggat aagatgcgat caattgcatt gtttctcatc
     4981 agaaagggcg tatatctttg tcagaaaaac gagatttatt tgaaaaactg ttggaacaaa
     5041 tccaactaga ggaagcagaa aaaaatcatc cactgcttac tgctggtgaa atggatagtg
     5101 tagtggtcca tcgtaaatcg cgtttatggg aatttacgct gcatttttcc aaaattttgc
     5161 ctattatgct atatcggtca ttgactcagc atctgactat cgcttttaaa gatattgcac
     5221 aagtcaaact gaatatctta gcagatgacc agacattcga tgaacagctc ttgcaagatt
     5281 actgggccca agctttggaa aatcagcaat gcgatacacc actagcacag caagtattga
     5341 aaacgcaggt acctgtgata aaagatcgta aagtcatctt gccaatcgac agtacaggag
     5401 ctatttctta tttgaagcaa caatacttgc cattagtaga agagttgttc gtcagttacg
     5461 gattcccaaa atttcgtatc gaaccagaag tggatgagca gcaagcggaa cgagtattga
     5521 aactttttga agaacgcaaa caagaacagg cagaagcctt tatgaagcaa gcagctgaaa
     5581 gtctagttgt ccacgaacaa aagaaaaagg aaagaaaaga gcaatcgcca gctcttgaag
     5641 gacccatcca catccagcta ggacgaaata ttccagccga tgaaccaacg acaccaatga
     5701 tcagtatcgt tgaagaagaa cgaagagtca cacttgaggg gtacgtattc gacaaagaag
     5761 tcagagaact acgttcaaaa cgcaagatct tgactttgaa aatcacagat tatacttcat
     5821 catttatcgt caaaaaattc tcgaacggcg aaaaagatga acaagtattt gatgcgatca
     5881 gtgtcggtag ctggttaaag gtacgaggaa gtattcaaga agatacgttt gtccgtgatt
     5941 tggtcatgaa tgcgcaagat atcatagaag tcaaacatac acctcgcaaa gattatgctc
     6001 ctgaagggga aaaacgagta gagcttcatg ttcatagcaa tatgagtacg atggatgcaa
     6061 caaacagcat ctctgatctt gtggcacaag cagggaaatg gggacaccga gctatcgcca
     6121 ttacagacca cggtggtgcg caagcctttc cggaagcaca tagtgctgga aaaaaagcag
     6181 gtgtgaaaat cttatacggc gtggaagcga atgtcgtaga tgatggtgtc ccaattgctt
     6241 ataatgatgc acatgaagca ttgagtgaag ccacatatgt cgtatttgac gtggaaacga
     6301 caggattgtc tgcagtatat gacacgatca tcgagctagc cgcagtcaaa atgtataaag
     6361 gaaatgtgat cgaaagcttt gatgaattta tcgatccagg acatcctttg tcacgaacaa
     6421 cggtagatct gactggtatc acggatggaa tggttcgagg atcaaagtcg gaagaagaag
     6481 tattgcggat gttccttgaa ttttcaaaag atacaatttt ggtagcccac aacgcggctt
     6541 ttgatatggg gttcttgaat actagctacg caaggtatgg tattcctgaa gcggccaatc
     6601 cagtcattga tacgttggaa ttagctagat atctgtatcc tcaattcaaa cgtttcggat
     6661 taggtgtgtt atctaaaaaa tttggtgtca gcttggaaca gcatcaccga gcaatctatg
     6721 atgctgaagc aactggtcac ttagcatgga tctttgtgaa agaggcaatg gacaatcata
     6781 acatgcttta tcatgatcag ctaaatgagc atatcggaga aggagattct tacaaaaggg
     6841 ctcgaccttt ccatgtaacg attttagcaa aaaatcaggc aggactgaaa gatttgttca
     6901 aactgatttc aatgtcgaat gtcgaatact tcgaacgcgt cccacgaata ccacgctccc
     6961 aattgaaaaa gatgcgtgaa aatctattga tcggctcagc ttgtgataag ggagaaatat
     7021 tcgaagccat gatgcaaaaa ggggtagaag aggcaagaaa ccgagcaaaa ttctacgatt
     7081 atattgaagt tatgccaaaa gcggtctatg cgcctttgat cgagcaagaa ttggtaaaaa
     7141 atgaacatga tttggaagaa attatccaaa atttggtaga aatcggaaaa agtttggata
     7201 aaatcgttgt tgcaacaggt aatgtacatt atctgaacga agaagatgcc atttaccgaa
     7261 aaattttgat caattctatg ggcggagcga atccgttgaa tcgtcatagt ttgccggatg
     7321 tccatttccg aacaaccgat gagatgctaa ctgcatttca ctttttaggg gaagaaacag
     7381 cgaaagagat cgtagtagaa aatacgaata aaatcgcaga tatctgcgaa gaagtgattc
     7441 cagtcaaaga tgaactttat acaccgaaga ttccaggttc agaagatgaa atatcagaat
     7501 tgagttacac aaaagcaaaa caaatgtacg gagatccgtt acctgagatc attcaaaaac
     7561 gattaaaaaa agagttgaat tctattaatg gtaatggctt ctcggttatt tatctgatcg
     7621 cccaaaaact agtgcataaa agtaatgaag atggctatct agtaggttca cgtggctctg
     7681 ttggctctag ctttgttgcg acgatgacag gaatcactga agttaatccg ttagcccctc
     7741 attattattg tccagagtgc caatattccg agttttttga agatggtacg tatggttctg
     7801 gatttgacat gccagaaaaa caatgtccaa aatgtggtgc acgcttaaac aaagacggtc
     7861 atgatattcc atttgagaca tttttaggat tccatggaga caaagtgccc gatattgatt
     7921 tgaacttctc aggtgattat caagcagaag cccataacta tacgaaagta ttgtttggcg
     7981 aagactatgt ttaccgtgcc ggaacgattg gtacagtcgc cgacaaaaca gcatatggct
     8041 atgtaaaagg atacgaaagg gacaataatc tgcagtttcg aagtgcagaa gtcgacagat
     8101 tagcaaaagg cgctactgga gtcaaacgga caaccggcca gcatccaggg gggatcatcg
     8161 ttattcccga ttatatggat gtctatgatt ttacgccgat ccaatatcca gcagacgatc
     8221 aaaattcgga atggaaaacg actcattttg attttcactc gattcacgat aatgtattga
     8281 aattagatat tctagggcat gatgacccaa ctgttattcg gatgcttcaa gatttatcag
     8341 ggatcgatcc acaaacgatc cctacggatg acccggaagt gatgcggata tttgccggtc
     8401 ctgaagtact tggagtaagt caggaacaaa tttattccaa aacagggaca ttaggtattc
     8461 cagaattcgg cactcgtttt gttcgaggga tgctagaaga aacgcatcct acaacttttg
     8521 cagaactact gcaaatttcc ggactatctc atggtacaga cgtatggtta ggaaatgcgg
     8581 aagaattgat tcgacgagga gatgcgacac tggcggaagt aatcggctgt cgtgatgata
     8641 ttatggttta tttgatccat gctggattag atagcgggat ggcatttaaa atcatggaaa
     8701 ctgttcgtaa aggacaatgg aacaagatcc ctgatgaatt gcgagaaacc tatctatcgg
     8761 cgatgaaaga aaataatgtg cccgactggt atatcgattc ttgttcaaaa atcaaatata
     8821 tgtttccgaa agcccatgct gcagcttacg tattgatggc tcttcgtgtc gcttatttca
     8881 aggtttattt ccctattttg tattactgtg catacttttc tgtacgtgca gacgactttg
     8941 atctagtctc catgtgcaaa ggaaaagatg ctgtgaaaca agcaatgaaa gaaatcacgg
     9001 acaaaggatt agatgcttcc gtaaaagaaa agaatcaact gacagtgctg gagttagcca
     9061 atgaaatgct ggaacgagga ttcaaatttg gaatgattga tttgtataaa tcggatgccg
     9121 ttaactttgt tattgaaggt gatacgttga tcgcaccatt tagagctgtc ccaagtttgg
     9181 gaaccaacgt ggcaaaacaa atcgttgaag caagaaaaga cggtccattt ttatccaaag
     9241 aagatcttgc cacccgcggt aaagtttcaa aaacattgat cgaatatatg aacgacaatg
     9301 gcgtattgaa agatctacca gatgaaaatc aattatcgtt attcgatatg ctataagcat
     9361 ctgttttcct gtctgcttgt aattttaagc agagtatggt atagttctta taggtaatga
     9421 tactaactgt gagtgagcgg atttttcgct cactcttttt aatggaatta cgcacttaat
     9481 tttttaagga ggcgaaactt ttgagtagcg tcgttgaaac agtcacagaa atggtgacac
     9541 cgattttaga tgaacaaaat tttgaacttg tcgaagtcga atttgtaaaa gaaggaaaaa
     9601 gctggttctt acgagtattt attgacaaag aaggcggtat cgatatcgaa gaatgcgcct
     9661 ttgtcagtga gaaactgagt gaaaaacttg atgcagcaga tccagatccg attcctcaag
     9721 cttatttcct ggaggtatcc tccccgggag cagagcggcc attgaaaaaa gaaagcgact
     9781 atgaaaaggc acttggtgat tatgtccatg tctccttata ccaaccagta gatggcgaaa
     9841 aacaatatga aggatttctt caatcatttg atgctgaaca gcttacattg aaaatacgca
     9901 taaaaacacg agaaaaagaa ttgacttttg accgaaaaaa tattgcaaaa gctcgcttag
     9961 ctatccaatt ttaatcacgg agaggaatca gaaaaaatga gtaaagaaat gttaaatgcg
    10021 ttagacgctt tagaagctga aaaagggatc tcaaaagaaa tcgtgatcga tgctttagaa
    10081 gcagcattgg tttctgcata caaacgtcac tatggccaag cacaaaacgt cgaagtggaa
    10141 tttgatcaga aaaaaggaaa gatccatgtc tatgcggtaa aagaagtaac ggaagaagtg
    10201 atggattcac agttggaagt ctcattgaaa gatgcacttt taatcaatcc agcatatgag
    10261 atcggtgata cgatccgttt tgaagtaaca ccaaaagatt ttgggcggat cgctgcgcaa
    10321 acggctaaac aagttatttt acagcgtgta cgtgaagcag aaagaacgat tatttataac
    10381 gaattcagtg cttatgaaaa agacatcatg caaggaatcg tcgaacgcca ggataaacgt
    10441 tatatttatg tcaatcttgg taaaattgag gcagttcttt ctaaacaaga tcaaatgcca
    10501 aatgaatttt atcaaccaca tgatcgaatc aaagtttatg tttcccgagt agaaaatacg
    10561 tctaaaggtc cgcaagtatt tgttagccgc agccatccag acctattgcg ccgcttattt
    10621 gagcaagaag taccagaagt ctatgatgga atcgttgaaa ttgtcagcat cgcccgtgaa
    10681 gctggcgatc gttcaaaagt ggcagttcgc tcaaatgatc caaatatcga tgcagtcggt
    10741 acatgtgtcg ggccaaaggg acaacgagtt caagcaatcg tcaatgaatt aaaaggcgaa
    10801 aacatggata tcgtcgaatg gaatgaagat ccagctgtat tcattgcaaa tgcattgaat
    10861 cctgcacaag tcatggatgt gatcttcgat gagacaaatc caaaggcctg cacagttgtc
    10921 gttccagact accaattatc gctagcaatc ggtaaacgcg gacaaaacgc acgtttagct
    10981 gctaaattga ctaaccataa aatcgatatc aaatcagaat ctgacatgac ggaattttat
    11041 gaaaatcaag aacaacaaaa agaaacagaa gaactgcatg atgaagcaat cgttcagtca
    11101 gatttaacag acgatgaata tgaaacaatt gcattcaaca atgaaacggt cgaagaaaaa
    11161 ccagaagctt aactttagta aggaggcaaa gagatgaaac aaaggaaaat tcctttacgc
    11221 aaatccgtgg tttcaggtga aatgaaacct aaaaaagaga tggtccgaat cacccgttca
    11281 aaagaagggg aagtatcgat cgatccaact ggaaaaatgc ctggacgagg agcgtatgtc
    11341 tcacttgagc cggaagaagt acaacaagct tgggataaaa aaattctcga tcgagtactc
    11401 gaaacaacat taacagatga gttttatcaa gaacttcttg actatgtcac tcaccaaaaa
    11461 gcacgtaaag agttatttgg aaaatgaata cagttaatcg gcaaaaagcc atgaatctca
    11521 tcggtctagc gatgcgcgcg ggaaaactaa tcacgggtga agaactgacc attgctgaaa
    11581 tccgcaaaca aaaagcaaaa ttggtgtttg tggcaaccga tgcaagcgag aatacaagaa
    11641 aaaagataaa agataagagt tcatattatg aagttccttg ctttgagcta ttttctgaag
    11701 cagaaatcac gcagatgatc ggaaaacctc gcaaggtatt cggaataatg gatgccggat
    11761 ttgcgaagaa gaccaaggag ctaattgaag gttaggaagg tgattgcatg ggcaacaaaa
    11821 gaatctatga attagcaaaa gaactcaaaa aatctagtaa agacgtcgtg gaaaaagcac
    11881 aacaacttgg catcgatgtg aaaaaccata tgggtgcgat tactagcaat gatgagaaga
    11941 aattgcaaca agcgttcaaa aacccaagca cgaaacaaca agctgcacaa caagcaagca
    12001 gaaaaccagc caatcaacag ccgaacgatc aacgtaaaaa agaaacaacg aacaatcaac
    12061 aaaaaaataa aaataaccgc agttatcaag atcgcggtca agggagcggt caagtgaatc
    12121 aaggaaaaaa accaacaaca aaccaaaata atacgggaaa taccagcagc caaaatcgtc
    12181 aaggaacgag ccaaggcggc aatcaaaacc gtactaacaa taacaacaaa ccaaacaata
    12241 acaatcgcgg caaatttaat aataataacc gtaatcgtta taataaaaaa ggtaaaaaag
    12301 gcaaacagca acaatcaact aaaccagctg taccaccacg caaattccgt gaattgccag
    12361 atgtattaga gtatactgaa ggtatgaacg tagcagatat tgcgaaaaaa attcatcgtg
    12421 agccagcaga aatcattaaa aaattattta tgcttggtgt catggtcaat caaaaccaag
    12481 cactggataa agatacgatt gaactgttag cgacggacta tggcatggaa ccacaagaaa
    12541 aagtccaagt tgacattgct gatatcgata aattcttcga ggcagaagaa gtcgatccag
    12601 ataaattagt ctcacgtccg ccagtcgtaa cgattatggg acacgtagac catgggaaaa
    12661 caactttatt ggatacatta cgccattcac gtgtaacaag cggcgaagcc ggcggaatca
    12721 cacagcatat cggtgcctac cagatcgata ttgatggtaa accaatcact ttcttggata
    12781 caccaggaca tgcggccttt acaagtatgc gtgcacgtgg agcaagtatc acagatatca
    12841 cgatcttagt tgttgctgca gatgatggcg tcatgccaca aacagttgaa gcaatcaacc
    12901 atgccaaagc tgcaggcgtt ccaatcatcg tcgctgtaaa taaaattgat aaaccaggtg
    12961 caaaccctca acatgttatg caagaattaa gtgagtatga gttaattcct gaagcatggg
    13021 gtggagaaac gatctttgtt gagatctcag cgaaattcgg ccaaaacatc gaagaattgc
    13081 tggaaatgat cctgttagtc gcagaagtag aagatttaaa agcagatcca acacaacgcg
    13141 caatcggaac agtgatcgaa gcacgtcttg ataaaggtaa aggtcctgtg tcaaccttac
    13201 ttgtccaaga aggaacactt catgttggtg acccaatcgt tgtcggtaat acttacggac
    13261 gtgtccgtgt aatgacaaac gatcttggcc gtcgcgataa agaagcagga cctgcaacgc
    13321 cagtagaaat cactggcttg aatgatgtac ctcaagcagg tgaccgtttc gtagtcttcg
    13381 aagacgaaaa gacagcacgt gcagcgggtg aagaacgcgc gaaacgtgca ttattagaaa
    13441 accgtgctgc cacaagccgt gtgactttgg ataacttatt tgaaagcctg aaagaaggcg
    13501 aattgaaaga agttaacgta atcatcaaag cagatgtaca aggttcagca gaagcactgg
    13561 ctgcatcatt gaagaagatt gatgtggaag gtgtccgtgt aaaaatcgtc cattcagctg
    13621 tcggagcaat caatgaaagt gatgtaacct tagcagcagc cagcaacgct attatcatcg
    13681 gtttcaacgt tcgtcctaca ccgcaggcaa aagtgcaagc tgatactgaa gaagtcgata
    13741 ttcgtcttca ccgtatcatc tataaagcca tcgaagaaat cgaaactgcg atgaaaggga
    13801 tgctagaccc tgaatttgaa gaaaaaatca ccggtcaaat gatcgttcgt gaaaccttca
    13861 aagtttctaa agttggtacg attgcaggtg cctatgtaac agaaggatat attcgtagag
    13921 atagcggtgt ccgtgtaatc cgtgacggaa tcgtaatcta tgaaggccaa cttgcaagtc
    13981 tgaaacgatt caaagatgac gtgaaagaag tcaaaatggg ttatgaatgt ggtgcaatga
    14041 ttgaaaaatt caatgacatc aaagtagatg acgtaattga aggatttatc atggaagaag
    14101 tcaaaaatga ctaaaaactg ataaaaggag gccaatcatt atggctaact accgtgaccg
    14161 ccgagtcggt caagaaatct taaaagaagt caatgatatt ttgcgcaaaa aggtccgtga
    14221 ccctcgtgtg gagaacgtaa cgatcacaga tgtgaaagtt acgggggact tacagcaagc
    14281 aacgatctat tacagcattt tatctgactt agcatcagat aagaaaacag cacaagatgg
    14341 attgaataaa gcatcaggat tgattcgtcg agagttaggg cacaatctaa gtatttataa
    14401 gacacctgaa ttgttctttg aacttgatga atcagttgtc tatggcaacc atatcgatga
    14461 attgcttcgc gacttgaaca aagactaatc aaaaactccc gcaaatcctg tgaaatcagg
    14521 ggttgcggga tttttttgtt ttcattaaga cgtattacct aagagttcca tgactttagt
    14581 cttgcggcgt gtcagctaac caaaaaagag aaaaagaaaa gtaaaaaatt cgactttctt
    14641 ttttctagta gtatataaat ttttgcatat agataaagat caagataccg ataacggaag
    14701 ctagattgta atctatttta gtgacagtca ggtatagcca agtaactaga agatcagtgg
    14761 aacctgcacc ataaaagttt gagtttgtcg gtccgttgac agttaaaaga aaaatcacgt
    14821 tgaagttatt gatatttccg attacgccgc ttcgatttgg tcacgatcca aattttggat
    14881 aatccctgta gaaattagca tagtagctgg gataccaatt cacatattga caaagataac
    14941 agagactttt acataaaacg gatcaatcag gaaaggaatt gctcgatcca gccccagttt
    15001 tccagcaatg tgttaactgg cccagcgtca ttcagcaaat tatgcatgat gaggagcgaa
    15061 atgaactgcg gaacagccat tgtgataacg acaagttttt tggatcaatg taaaaaacgt
    15121 cagcgaaagg aaaaatttcg tggtttttta tacctgttca tttggctaga tataaaatcc
    15181 aaacaaatga acagaagtgc ttattaggtt tcaattggga gtatctgcgc tagaataaga
    15241 gtaataatga aaaggagtgt tatgtatgga ttattctagt aaagaatact ttgataaaat
    15301 gacggcttgg tggcgtgctg ctaattatct ttctgttggg caactttatc ttaaagataa
    15361 ccccttactg agacgtacac tgaaacccga agatgtcaaa aaacatccaa tcggtcattg
    15421 gggaacgatt cctggccaaa attttattta tgtccatttg aaccgagtca tcaataaata
    15481 tgatttgaac atgttttata ttgaagggcc aggacacggc ggacaagtca tggtatcgaa
    15541 tgcttattta gatggaagtt acacagagat ctatccagaa gtaacggaag atgagacagg
    15601 aatgcaaaaa ttattcaaac gattttcatt tcctggaggc attgcttctc atgcagcacc
    15661 agaaacacca ggttctatcc atgaaggcgg agagcttgga tactctcttt cacatgcagt
    15721 cggagctgta ttagacaatc ctgaagtaat ctctgctgtc gtgattggtg atggtgaagc
    15781 agaaaccggt cctttagctg gaagctggtt ctcaaatgtc tttatcaatc cagtgataga
    15841 tggagctgtt ttaccgatcc ttcatttaaa tggtgcaaaa atagccaacc caacgatctt
    15901 ggcacgtaaa tcagatggag aattagcaaa ttactttaac ggacttggct gggaaccttt
    15961 ctttattgaa ggaaatgatt cagaaaaatt aaatcctgtt atggctgaaa aaatggatca
    16021 agcaatcgag aaaattaaat ccatccaaaa agaagctcgc ctgaagacag caacagatgt
    16081 cgtgatgcca aaatggccag tattgatcgt acgcacacca aaaggatgga ccggtccaaa
    16141 agaatgggac ggggaaccaa ttgaaggaac cttccgagca caccaagtac cgattcctgt
    16201 agatcaagaa catatggatc atgcagatgc tttactccgt tggctgaaat cctatgaacc
    16261 agaaaaatta tttgatgcac aaggacgtat tttagaagaa atcagagaaa tcgcgcctac
    16321 tggtgaccaa agaatggcaa aaaatcctat caccaatggt ggaatcgatc caagaccgct
    16381 tattatgcca gattggaaaa aatatacttt gcaatttgaa aaaccaggct ccgttaaagc
    16441 agaagatatg accgaacttg gtaaattcgt tcgcgagatc attgagaaaa atccagaaaa
    16501 tttccgtata tttggaccag atgaaacaaa atcaaaccga ttgaatcaag ttttcaaaac
    16561 gacaaatcgc caatggatgg aaaaaatcga accagaaaat gatgaatggc tttcgccatc
    16621 tggtcgcgtc atcgacagtc aattatcgga acaccaagat gaagggttct tagaagggta
    16681 cgttctgact ggacggcatg gttttttgca agttacgaat cattcttgcg agtggttgac
    16741 tctatgttga ctcagcactt caaatggatg cggaaatcac atgacctttc ttggcgaaat
    16801 gattatcctt cgctaaatct cattgcaagc tcaacagtct tccagcaaga tcacaatgga
    16861 tatagccacc aagatccagg aatattgaca catttggcag aaaagaaggc ggagttcatc
    16921 cgtgaatatc tgcctgcgga tgcaaataca ttgctcgcag tcatggataa agcattccgc
    16981 tcgtcagaaa aaatcaattt gatcatctct tccaaacatc cgcgtgctca attttactca
    17041 gcagaagaag cagccgtctt agtgaatgaa ggcttgaaaa tcattgattg ggcttcgaca
    17101 gcaaaagagg aggaacctga attggtcatt gctgctgcag gaacggaatc taacttagaa
    17161 gcattagcag ctgtaacttt gctgctggaa gaattcccaa aactaaaaat tcggttcatc
    17221 aatgtcgtag atttattgaa attacgtcat ccaagtcaag atccgcgtgg gttgagcgat
    17281 gaagaatttg atcaatattt tacaaaagac aaaccgatct tatttgcttt ccacggttat
    17341 gaaacattgg tccgcacgat tttctttgat cgtcataacc atcatttaat gattcatggc
    17401 tacaaagaaa atggagatat cacgacacca tttgacatgc gtgtagtaaa tgaacttgat
    17461 cgttatcatt tggcaaaaga cgcagcttta aaaatcaaag gaagtcaagc agaagatttt
    17521 gcgaaaaaaa tggatcagaa attacaagaa catcaaaact atattcgtga aaatggtatt
    17581 gatctgccag aagtattgga ttggaaatgg aaaaatcttg accaataaaa tgataaaatc
    17641 gatcccgcaa aatagaattt tttacattga ttaagaaact gaaaaaataa ataaaaaaaa
    17701 cggaaaaaag actggaagaa tggccagtct tttttttcgt ttttgggaag tgaaaaccaa
    17761 actatttacc tgtaagccac ccataaatcc aatcaaatgg tgcaaagaaa aaatcttcca
    17821 ttctattccc tcctttaaaa aagcataaca tgatttctat ttacgtgagc atgaattttg
    17881 ttacgttttt attgccgaaa aataaaaacc gttcattatg atgtcaaatt cgtcagtaat
    17941 tgatgcaatg tgttataata attcagttaa atcaaaaatg aaatggagaa aagacatgga
    18001 cgggttatta cctttatgga aagaacgcgg aatgacaagt catgattgcg tattcaaatt
    18061 aagaaaaatc ttacatacga aaaaaatcgg acatgggggt acgcttgacc cagacgtaga
    18121 cggtgtgttg cccatttgta ttggaaaagc cacaaaagtt atcgaatatt tgacagattc
    18181 tgggaaaatc tatgtaggcg agataactct aggtttcagt acaacgacgg aagatcgatc
    18241 cggagaacgg gtagaaacaa aaccagtaga tatgtcttta tctgaaaaaa aatagatgaa
    18301 gtgatggctt catttattgg tgagatcacc cagataccac caatgtactc agcggtaaaa
    18361 gtcaacggta agcggttata tgaatatgcc cgcaataatg aaacagtgga gcgtcctgtt
    18421 agaaaggctc agatttaccg ttttatccgg acaagtgaat tgacaagaga cgaagtgaca
    18481 ggaaccattt cttggcgctt cgaagtagaa tgcgggaaag gaacctatgt ccgcacatta
    18541 gctgtagata caggcaagaa acttggttac cctgcgcaca tgtctgatct gacccgaaca
    18601 gcaagtgccg gtatgtccca gaaggatgct ctgacattag aagaagtagc tcaagctgtg
    18661 aaaaatggga caatcgatga ttgtttgctg ccaatagaaa aaggagtgga acggttcaaa
    18721 cgagtagata ttactgatga agtatggcag aaagtaaaaa acggtatgag acttgattat
    18781 actgtatttg cgctcccaga aatgccgtca gaagaaattg ctttgtttta taaaggaaaa
    18841 gttgtcagta tttatcagcc aaacccgaaa gaaaaaaaca aattaaaacc tagtaaagta
    18901 ttgagaaatg aggaataacg aatgaatatc atcaagatca gacatcctta ccaatcagaa
    18961 caaattccag aagacgatgt cgttttagtg ctgggatttt tcgacggcgt gcatatcgga
    19021 caccagaaag tcataaagac aggtatggaa attgcacaaa aagaaggatt gaaattagca
    19081 ttgatgactt tcaatcagca tccatctatt gtctttaaaa agatcgatcc tagcagcgtg
    19141 aaatatttga ctagccttaa gcaaaaagaa gaaaaaatgg ccgctttggg tatagattat
    19201 ttatatgaag ttgattttac ttcttctttt gctcatttag caccgcagga atttgttgat
    19261 caatacatcg tagggttaca tgcaaaatat gctgtttccg gttttgatta tacgtatgga
    19321 ccaaaagaca ttgcagatgt cgctcatttt cctgaatatg ccaaaggaag attcaaaatc
    19381 gtcacagttt ctaaagaaga agaacaaggt gaaaaaatca gctctacccg tattcgtgga
    19441 ttactagaca gcggacaagt aaaagaagca gcaaaactat taggttgcac atatgaggta
    19501 gaaggggtag ttgtccatgg cgatgcacga ggcagactac taggattccc tacagcaaat
    19561 gtcaaagtaa agagcacggt tcatttgcct aaagaaggtg tttatgtgtc ggaaataaaa
    19621 attggggaaa catggtatcc agccatgggt tcaattggac ataatgatac attcggtgaa
    19681 ggacgggagc tgactgtaga gttgtatatt ttggacttta atcaagatat ctatggtgaa
    19741 catgtcgcgg tacgctggaa tcaattttta agagatcaag tgaaattttc tagtgtagag
    19801 gaattgattg aacaactcaa ggaggatgaa caagcaacag ctgaatattt ttctcagcaa
    19861 taaaacatta tgcataaaat ctcttgttta tgtataacta gcaattacaa taaaatgggt
    19921 gataaataag cccaggaaag gggctgtgac aaaagtcttg tcacagcctt tttttccaaa
    19981 taaatggttt ttcagaagta gcttcttcgg aaataagccg aaatttcttg aagctgacca
    20041 cttctgtcac aacctctttc tttatagaaa taaaaggaca ctagcaattc atgaaaacga
    20101 ttgctagagc gtatcatttg ctttagacta tatatcttgg tgtacactca ctttaaggcg
    20161 gaaataaaag ttatccgtaa taacaggttt aggtatgaaa aaggagtggg tactatgtac
    20221 aaggaattag aaaacaaagt cgcagtagta actggtggct caaaggggat tggaacagcg
    20281 attgctcaac gattcgggaa agaaaaaatg aatgtagttg tcaactataa ctcggataaa
    20341 gaaggagcag aaaaagcagt caaagcaatc gaagaggcag gcggaaaagc agtctctgta
    20401 caagcagatg tcagttctga agaaggaata cagcgtttac ttgatacggc tttagaaaat
    20461 tttgggacac tggacgtttg ggttaataat gctgggatgg aaaatcagca tccaacccat
    20521 gaacttagcc ttgaagattg ggaaagagtg ttaaaagtca atttgactgg cgtttttatc
    20581 ggaacgaaag cggctttaaa ttactttaca gagaataata aaaaaggaaa tatcatcaat
    20641 atgtcttctg ttcatgaaca gatcccatgg ccaacgtttg ctcattacgc tgcttcaaaa
    20701 ggcggggtaa aattatttac cgaaacggta gcaatggagt atgcaccgaa gggaatcaga
    20761 atcaatagta taggccctgg tgctatccgt acacctatta atgcggcaaa atttgatgat
    20821 cctgaacaaa aagaactctt ggaaagtatg atcccactag gacgcgtagg tgaaccaaaa
    20881 gaaatagcgg cgacagctgc atggttggct tcagacgaat ccagttatgt tacaggtatt
    20941 acactatttg cagatggtgg gatgacttta tatccttctt tccaaggtgg aaaaggataa
    21001 gggtgaataa aaatgggttt attgattgca ttgatcccgg cagttggctg ggggatccag
    21061 ccactgattt tgaaaaagat tggtggcaga ccaacaaatg agatattagg aactggtatt
    21121 ggagcggtgc ttgtcggctt actcgttcaa ttattcgctt ctccagctgg tatttcagtg
    21181 actacatttt tgattagttt actttctggt gcattttggg ttattggaca aacaggacag
    21241 tatacatctt tcaacttgat tggtgtttct aaaacgatgc caatttcgac tgcattacag
    21301 ttagtcggaa cctcgttgat tggtgtcttc gcttttggag aatggtctgg aagtatgggg
    21361 aaaatcattg gagcagcagc aatcattctt cttgtatttg gttctgcttt aacggcaatc
    21421 agtgatgatc ctggaaaaaa aggcggtatc acaaaaggaa ttagtctatt agcatcaaca
    21481 agtattggtt attgggtata cagtgcattg cctaaactag ttgatgcaag cggactttcc
    21541 attttctttc cgcaaatgat cggtgtcttt ttaggggcag tgatttatgt gattttccgt
    21601 aaaccaaccg cattttctga agacaagagt tggaaagcaa cgatcgttgg agccatcttt
    21661 agtgtgtcag cattggctta tattttttca gcaaatgaaa atggggttgc tactgcatat
    21721 atcattacac agctaaacgt agttatctct acattaggcg gtatgtacat cctgcatgaa
    21781 aataagacac caaaagaaaa aaaattaacg attatcggat tattgctgat cgtagtagga
    21841 agcatggtca cggtctttat ttgaggataa agatggtaaa atttcattta ttaaaaaaat
    21901 atttgtttga accaaacagt aagtacttat aaaaaaatag cgctagaatt tccaacatct
    21961 ctggaatttc tagcgctttt taattgttta agatacaagg gaaatatctg tctggatcaa
    22021 taatttttca ttgatccatt gactaaagtt tacgacataa ttgaagttac caccgattag
    22081 tatctccgca tgattgtctt tataaagatc gtattgtaaa agacggtctt ttttatttgc
    22141 gtaaacatcg ttatggtact ggactaattg ttctcggaat tgattgattg ctgtaaaatc
    22201 acgatattga aggttgaaac gcatcgtgat tggtgcattt agcatgaatc gctgcatttc
    22261 ttttgctgct tcctctgctt gatctccgta taggaactca aaaagattac gttcagaaaa
    22321 tttccattgc ccgccaacct ttgttccttt tagttttcct tcatgaagat aatttctgat
    22381 agaacgtgta gtaacaccta attgttcggc taattcattt actgagtaaa aatctttcaa
    22441 tgttcaccct cctatgtgaa tggttctact gacttagttg atatttgctt aatcacgaaa
    22501 tgtagaaaga acgattttaa ttaaggtcat tgtaaaaatc aagtcagaaa attctaactt
    22561 ttatttctct aaagaaattc gttctctcct aaagtgatat tatatttaaa tatacctgaa
    22621 aataatgggc agtattatga aagatcactg atattttctc gtattttttc cttattcaaa
    22681 agttttgcag gaaaattgct gaaaaaataa ttttactatg tagaaaattc aaagccattc
    22741 aatcagtgat ttttttcagc acaagaaaga ctccttataa aaatagcaga tactttgtca
    22801 attaagtgtt taaccaggga tgataaaaag gataggattg aacaaaacct gtataaggtg
    22861 atgaatagtt tcctcgccag taaaaggttt attcgtataa agctttatcg ccaaaattta
    22921 tcatgacctt ttttatcttc aatattaaat gaaatcaatt gttcaatcaa ttcccagttg
    22981 attacacttt tccaaggaac tttgatgacg ttatccgtat atacgtagcc tgcttcttgg
    23041 atattttttt caagggcacg aatcgctgct ttttccggtg cgatagaaaa atgattttta
    23101 gcaaaactaa atgcaataat aaaggtacca tggtcagtaa acataggttg gttccatttt
    23161 acagttgtgt ctagctgggg atacgtttct gttatcttag caaaaaggtc tttgatcttt
    23221 tgttgatgtt ctttttcttt gatttgattg atgatcggct gaaatttttc cattctaaaa
    23281 cgcctccatt aagtggtttg atttatttta ccagaaaaac aatagttatg tataaaaaac
    23341 gaacaaaaca caagtaatgt tgtaaccgct atatttatat gataagatgt ctcaaataat
    23401 aaaatagaaa agagacataa aaatgattga cttggcaaag aaattcttac acgaaaatat
    23461 atattttgag ttgtatcatc atcgagccat ttatacaaat gaagatgctt tggtaataaa
    23521 gaaagaacaa ggatttacag gtacagagac caaaagctta ttcttaaaag ataaaaaaga
    23581 taataactac gtatatctga cttttactac gaaacaaacg gatttcaaag cattagggaa
    23641 gatcgtcggt aaaaggcttt ctattgtttc atcagaacgc atggaagaac aaacgggaca
    23701 aaagcagggc gctgtttctc cgtttggtta tgaaaaacag attccgatta ttattgatga
    23761 agagctctta tcaaatgaaa aacttgtttt tgcaccaggt agaccagatc aaacgatggt
    23821 catccaaacg gttgacttaa acaaaatcat ccagctactt gagatgccga tatttttgat
    23881 gccggcaaac gaaaaaaaca attaaccaag taaaatagtg catacttgga ggtgaagcaa
    23941 catgataagt tatccattat tagaaagaaa tgcaacgatt ggggttactg caccatcctc
    24001 aggcgtatca gaggaacacc attcgttact cgaatcagct ttaaagagaa tggaagaaaa
    24061 atctttccaa gtagttactg gaaagacgct ttggacgcaa gataaggaaa aatcaggtcc
    24121 agcaagaaaa cgagcagatg aattcaatca aatgatgcaa gatgaaaata tccaaatgat
    24181 ctttccgact tggggaggag aattattgat tgagattctc gattttgtcg attttgatgc
    24241 tctacaacca aaatggatag taggttattc agatattagt acgttgctac ttgctgtcac
    24301 attaaaaaca gggatagcca ctgcacatgg aactaacctt attgatttaa gaggagagta
    24361 cttggatgaa acgacagcaa tgtgggaaag tgtactgaaa acaagagaag gagaatcgat
    24421 tctacagcaa tcttctgaaa agtatcaaaa agtgtggccc catgagcatt catcatcttc
    24481 tgttttccat ctcactgaac caacaaattg gcaatcagtc agcggagatg cagtaaggat
    24541 gaagggacga ttattaggtg gttgtattga tacgatcagg catttaatcg gtactcctta
    24601 tggagatgtc caatctttta aaagaaagta taatgatgga aagccgatct tgtggtttct
    24661 agaaaactgt gagctatcgt cagttgattt aagaagatca cttgttcaga tgaaactggc
    24721 aggctggttt gatggcagct cggggatcat gttcggtaga agtgcggcag aggatttgtt
    24781 agattataaa atgaaggatg tatacgaaga tctgtcaaag gaattaaaac taccgatctt
    24841 gtatgatatt gattgtggtc atgtaccgcc acaactgaca ttgattaatg gtgcctatgc
    24901 agaagtagcg gtagaagata gaaaagcgac tgttttgcaa acgtttattt aaaaaatttt
    24961 ttcctgcctt ggagtctatc aaagaccaat aaatctaaat agaaaagaaa gtgaaagaag
    25021 ctgacaaaat gctgtcagct tctttttgta taacatatta gaaagtctaa ttatctataa
    25081 ctgtcacatc gattaactgt ttcacgtcat tgggatttcc tgtaggaata gctactacag
    25141 atgcattatc aatgatttct tgagttgttt ctctaatgaa cgctggataa gatgtttgat
    25201 ctgcaggcat ttgccaatgt gaaggaactg gataactagt gattgtcccg tcatggttac
    25261 ttgaatacac taaaactccc tggtaactgt atttcccagt gagcataacc acatctttcg
    25321 gatagactgc actccctgca tcataagggg cgataggtgt tcctgcagga taatgaataa
    25381 catgtaattc gaaatcaccc gatgtatctc taaaagtatt acctctaata gctagccaaa
    25441 cacgtgcgta ctcaatttgt agatcagaat atcctgctaa cggatctatt gactgagaag
    25501 actgtgagga ggttgtgtct attgttgaag aagaaatatt tgtctcaact gtactactag
    25561 atgtagaaga agtattgggc tctgagactt tactgcttgt tgaagatgta ttcgtttcag
    25621 tagaagctga tttctgagta cttgattttt cagctgaact ggtttgtctg attgaacttt
    25681 tgtctgtggt cgcagatgga tcactatttt ctttactagg agacgtacat cctacaagga
    25741 ataaagagac caacaataaa ataaaaccta actttttcat ataaaatacc tcattatttt
    25801 tttaatacca gtatagcata atcctgaatt tttgaatccg taaaaccttg tctatgaata
    25861 taaatagagt gtatcttctt tgataaatgt cagaaaaagt gtgacaaata atcatcagta
    25921 aaattacttg tcacactatt gataaggagt atttaattga agagaatgat taggagaaag
    25981 gatctccgta ctttttatag taatcataga atccaagttt gtatagacga atcgtttttt
    26041 ctcttatttc ttctgtttca aaatggactg cttgaagaat caagtctgtg aattctatag
    26101 gagctgttcc attcgcagta atcaaattac gatctttcac agcttgttgt tctataaaat
    26161 cttctttatt ctgataattt tccgattcta accaaggaaa ttgcgcattt ccggtgtgtt
    26221 tgtaaccttg caataatccg ctctgagcta aataatcaac tgccccacaa atagcagcta
    26281 ctggtacgtc tgtttttaac aatttatcga ttatttgata taaatgatga ttttttatgt
    26341 cccatgaatt gccaccgatc ataataaaaa ggtcaacttt ttcaggtatt tgttcgatta
    26401 agtaatcgat ttttgttttt aatcctccaa tggaagtgac ctctgattgg atagaagcag
    26461 ttttgttttg ccatttctca ttttggttta gtgtgctagt aatataagca ccttcccaat
    26521 cggcatattc ctctaataga aacgtaatcg cagttttcat tttcaagctc tcctttacta
    26581 tctataacaa ggacaacagt tttaacctac agacattcat taaaattggg tagcgacagc
    26641 gaaaacaaca attcttaagt tttagacaga gtatacagat agtcaactta attaaatacg
    26701 aactaaataa aaatgacagg taatcatttt tgtttaccag cattataaca ttttgcatac
    26761 atggatgaca aaagaaccaa ttcccacttg cttgagtaca atattaaaag aaactggaag
    26821 gcaaaaagta attatagaaa cgaaaaataa atcatatcgg gcactcagac atgaaaacaa
    26881 ccctagagat atataactag gtaaccagtt taatgaaaga aaaaatcatc caagaagtcg
    26941 attcttggat tttttagtgg atttgttcgc caaatacgtt tagtatgcgt atatgagagg
    27001 ttgtaagcaa agcgttgtgg tctgagcact taaggaggaa tctgtgaaaa tagcttttca
    27061 tatttttgca gattttgact taatgccgaa ggtcagcttt gcgattaccg tttatcgaga
    27121 ggctgtaaaa gcaccgtgta gctccgatga caataagcaa gcatttttag agctaggttt
    27181 ccttgattct aaatatgcgg cttatttcaa tgagttggtg cttgaacgcg attttaagaa
    27241 ggtcgaaaac aggcacgact tttcgacgta aggagaaaga atttcaatga tagaaacaat
    27301 aaatcccgca gaaaacaagg tttctgcgta cattcatatc ccattttgcg agcatatctg
    27361 ctattactgt gattttaata aagttttttt agaagggcag ccagtagatg agtatgtgga
    27421 aatgctgctg aatgaaatga aaataatgct agaaagtcat ccgacagcga atttagaaac
    27481 actttacgta gggggcggga caccaacttc tttatctgct aaacaacttg atcgactgtt
    27541 atcaggtgct agagagatcc tgccttttca aaatggtaat gagtttactg tagaagcgaa
    27601 tccaggtgat ttgacccgtg aaaaacttca agtaatgaaa aactatggcg tcaatcgttt
    27661 atccatggga gtgcagacat ttgataatcg cttattgaaa aaaattggac gtaaacacac
    27721 cgcagaagat gtgtatcaaa ctatgcaatt tttagaagca gaaaatttta ccaatgtaag
    27781 catcgatttg atttatgcat taccgggaca aacactagaa ggatatctgg atactttgga
    27841 aaaagctttg gctttggatc tgcctcacta ttctttgtac tcattgatcc tcgaaaacaa
    27901 aacaatgttc atgaactggg tacgacaagg aagactcgaa ctgcctgatc aggaaacgga
    27961 aaccagaatg tttgaagaga cgattgaagc aatggaaaag catggacgtc accaatatga
    28021 aataagtaac tttgggctct ccggacatga atctcaacat aacctcatgt actggaacaa
    28081 tgaccactat tttgggtttg gagctggtgc tagcggttat cttggagata ttcgttaccg
    28141 caacaaagga ccaatccaac actatttacg cccattacga gcaaatgaac gcccggtatt
    28201 ggaagaagaa aaattgacac gaaaaaatca aatagaagaa gaaatgttct taggactaag
    28261 aaaaaaaaca gggattacta aaaaacattt ctatgatcgc tatcaaaaaa caattgaatc
    28321 ggtgtacggt gatgtgctaa gggaattaga gcaagaaggg ttacttgtta atgaagaaga
    28381 ccgtgtttat ttgacaccaa gagggacctt tttggggaac aatgtgtttg agcgcttttt
    28441 gtttgaaaaa tagtttaaag aatatttgtt ttttatctga atttaaataa aaaaagttca
    28501 aaaatacaat aaaggactat tgaaacgttg atataactgg atttacatca attatttaga
    28561 attagcactc ttgtgtttag agtgctaaaa aatggagtct ttctcttgac aggagaaggg
    28621 cttcttgcta tatttataag tggattagca cttagaatat atgagtgcta atgagaggtg
    28681 aggaagatgt taacaaagcg acaaaacgac attttacgtc tgatcatcca acactatacg
    28741 agtagtggcg tgcccgttgg ttccaaaaca ttgatgtctg agggagtcaa ggccagctca
    28801 gcaacgattc gcaatgatat gaaagcatta gaagatgaag gattgctgtt gaagactcac
    28861 tcttcttctg ggcggattcc atctgcttta ggctatcgtt attacgtgga tcatttgtta
    28921 aaacctgctc gcgtagcaga agatgatttg ctgctgattc gtcagtctct aggcagagaa
    28981 tttcatgaaa tcaatgagat tattaagcaa tctgctgaga tattatctga gctgacaagc
    29041 tatacgactt tttcattggg acctgaaata aaagaccgcc gactgactgg ctttcgtatc
    29101 gtacccttga ataatcgaca gattattgct atcgtcgtaa cagatatggg gaatgtagaa
    29161 agccaagtgt tcactcttcc cgaatatatg ggaagtcagg aactagaaaa aatggtacag
    29221 attgtaaacg accgtctagt cggagatcct ctgatgacgg tgtaccataa acttcgtacg
    29281 gagattccga tgattcttca taaatatttc caaacgacag aagggatgtc tcagttattt
    29341 gacaccgttc ttggtcatgt ttttgaagat aaggtttatg tcagtggaca aatgaatctc
    29401 ttggattatg agccccgtca agatcttgat cagttcaaat cgatgttttc ttttatgaaa
    29461 gactcagacg gacttacaca aatgatcact ccgatggata gcaatattca tatcaagatt
    29521 ggtgctgaat taggcaatga tttattgcag aatatgagta tgattcaagc aagttacgag
    29581 atagatggtc atggaagagg tacaatagcc ttgttagggc ctactagtat gccttattct
    29641 cggatgttcg gtctagtcga tgtttatcga agagaactag cagctaaact tgctgattac
    29701 taccgttcgc ttgacttgtc aaactcgtaa tgtgaataac gaagctattt gcagctgatc
    29761 gttctctctt tcttagtaag agcaatcaaa cagctgactg aataatgaaa ggaagactgt
    29821 atcgtgaaag aaaatcaaga agaactagat aaagaaatga cagatgctca gccagaaccg
    29881 gaaatcgatg tcgaagctgc agaagattcg ggtatttcag aagctgaagc ggaagaattc
    29941 gaaacagcga aattaaaagc tgagttagaa gaaatggaag ataaatactt gcgggctcgt
    30001 gctgaaatcg caaatatggc aaatcgagga aaaaatgagc gtgagcaatt acaaaaatac
    30061 cgttcacaag atttagccaa aaaattactt ccatcaattg acaacctgga acgtgcatta
    30121 gccacagaag tgtcagatga tcaaggggca ggtttgaaaa aaggcgtgga aatggtcttg
    30181 gaaagtttgc gtaacgcact ggaagaagaa ggaattgaaa aaatccctgc taaaggagaa
    30241 gcctttgatc ctaatcttca tcaagcagta caaactgttc cagcgactga agatacacca
    30301 gccgatacga ttgtagaagt actacaagaa ggctacaaat tacatgatcg cgtgttaaga
    30361 ccaaccatgg tcattgttgc acaataattg tgcggatata aaacttacac tttcaattaa
    30421 agaaggagac gttaattatg agtaaaatta tcggtattga cttaggtacg actaactctg
    30481 cagtagcagt attggaagga aacgaagcaa aaatcatcgc taacccagaa ggaaaccgta
    30541 caacaccttc tgttgtatca ttcaaaaatg gagaaatcca agtaggggaa gttgcaaaac
    30601 gtcaagctgt gacaaaccca aatacgattt catcaatcaa acgccatatc ggtgaagcag
    30661 gttacaaagt agatgtagaa ggtaaatcat atacaccaca agaaatctca gcaatgatcc
    30721 ttcaatatat caaaggattc gcagaagact atttaggtga aaaagtcgaa aaagcagtta
    30781 tcactgtgcc agcttacttt aatgatgcac aacgtcaagc aacaaaagat gctggtaaga
    30841 tcgctggtct agaagtagaa cgtatcgtca atgaaccaac tgcagctgct cttgcctatg
    30901 gtttagataa aacagatcgc gacgaaaaaa tcttagtatt cgaccttggt ggcggtactt
    30961 ttgacgtatc tatccttgaa ctaggagacg gtgtctttga tgtattgtca acagctggcg
    31021 acaaccatct aggtggggac gactttgata acaaaatcat cgactacatg gtagcagaat
    31081 tcaaaaaaga aaacagcatt gacttgtctc aagacaaaat ggctttacaa cgcttgaaag
    31141 acgctgctga aaaagcgaaa aaagatttat ctggcgtatc aagcacacaa atcagcttgc
    31201 catttatcac agcaggagaa tctggaccat tgcatttgga aatgacattg acacgtgcaa
    31261 aatttgatga attaacagct gacttagtag aacgtacaaa agtaccagta cgtcaagcgc
    31321 taaaagatgc aggtttatca caatcagaaa tcgacgaagt tattttagtc ggtggatcta
    31381 ctcgtatccc tgcagttgta gaagcagtac gtaaagaaac aggaaaagaa ccaaacaaat
    31441 cagttaaccc tgatgaagta gttgctatgg gtgctgctat ccaaggtggc gtgatcactg
    31501 gtgatgtgaa agatgttgtt ttacttgacg ttacaccatt gtcattaggt atcgaaacaa
    31561 tgggtggagt gttcactaaa ttgattgacc gcaatacaac gattccaaca agtaaatcac
    31621 aagtattctc aactgcggca gataatcaac cagcagtaga catccatgta ttacaaggtg
    31681 aacgcccaat ggctgctgac aacaaaactc taggaagatt ccaattgaca gatattccag
    31741 cagctcctcg tggtattcca caaatcgaag taacattcga tattgataaa aacgggattg
    31801 taaatgtatc tgcgaaagat ctaggtacac aaaaagaaca aaaaatcacg atcaaatctt
    31861 cttcaggtct aacagacgaa gaaatcgaac gtatggtaaa agatgcagaa gcaaatgcag
    31921 aagcagataa agctcgtaaa gaggaagttg acctacgtaa tgatgtagat gcattgctat
    31981 tctctgtgga caaaacattg aaagaattag aaggcaaagt ggacgaagaa gaagtgaaaa
    32041 aagcagaaac agctcgcgat gaattgaaag ccgctgttga agcaaacaac atcgaagaaa
    32101 tgaaaactaa acgcgatgca ttgaacgaaa tcgttcaaaa cttgactgtt aaactatacg
    32161 aacaagcggc acaacaacaa gctcaagaaa acccagaagc agctcaaggc ggagcagatg
    32221 atgtcgttga tgctgatttt gaagaagtcg atggcgacga taaataatca gttcactgaa
    32281 tttcgagaga aggccaaagt tgctactttg gctttttctt ttgaaaaata gtttgaagta
    32341 tcggtttttg tttcaaacaa aatatgatat gataacaagc gacgtgaaaa tgatgagaac
    32401 caataggagg aaagcctatg gcaacaaaac gtgattatta tgaagtctta gggctctcaa
    32461 aaggagcatc agaagatgaa ataaagaaag cttatcgtaa gctttcgaaa aaataccatc
    32521 ctgacatcaa taaagaacct gatgcggaag aaaaattcaa agaagtatca gaagcatatg
    32581 agattttaag tgacccgcaa aaacgcgctg catatgacca atacgggcat gcaggcacag
    32641 atccaaatta cggtgctggt ggagctggtg gcggatttgg tggattcggc ggcttctctg
    32701 gtggcggctt cggtggtttt gaagatatct tcgactcttt ctttggagga ggcggtcggt
    32761 cagtcgatcc aaatgcgcct cgtcaaggtg ctgatttgca atatacggtc gacttgtctt
    32821 ttgaagaagc gatttttggt gtagaaaaag aaatcaagta taatcgggaa gaaatctgtc
    32881 atacttgtgg tgggaacggt gcaaaaccag gcacacaacc aactacttgt cataaatgtc
    32941 atggtgcagg tacgattaat gtagaacgcc aaacaccact tggacgagtg atgagccgtc
    33001 aaacttgtga tgtttgtcat gggacaggga aagaaatcaa agaaccttgt ccaacctgtc
    33061 atggctcagg acatgagaag aaagcacatt cagttaaagt gaatgtacca gctggtgtag
    33121 aagaaggaca acaaatgcgt ttagctggtc aaggagaagc tggagaaaac ggcggaccat
    33181 ttggtgattt gtatgtggtc ttccgtgtag aagaaagtga tatctttgat cgtgaaggtt
    33241 ctgaaattta ctatgaattg cctttaagtt ttgtacaagc agctttgggg gatgaagtcc
    33301 aagtaccaac tgtccatgga gatgtgaaat tgaagatccc tgctggaaca cagacaggta
    33361 cgaacttccg tttacgtgga aaaggcgctc cgaagttacg tggtggtgca acaggcgacc
    33421 aacatgtcaa agtaaaactg atcacaccga aaaatctaaa tgaacaacaa agagaagcat
    33481 tacgtgcatt cgctgaagct ggcggcatca aagttgaaga acagcaagaa gacggtttct
    33541 tcgataagtt taaagatgct tttggcggga aaaagcggaa gtagtttttc tagtaaaaac
    33601 gctaagtcat taataattaa aaaccagacg aacaatcata tgttcgcctg gtttttttgt
    33661 agttgtcatg tttgtaccaa caaaaaagcc agctttagaa tatgataatc gtcatagttt
    33721 ctaggggtat aaccagtgag agaatggaac ttgttcaatc gatattgcaa ggtgttttta
    33781 tgaataaata attcttctga gcaatttttg atgcttccgt tatttttact gaagcatgcc
    33841 aaagtcttct tgaacaaatg tacttcttct tctgtcaggc ttttcaaaat acgttttaca
    33901 tataatgttc gttgatctga cgggattgct ggtaaaacga tcccgaggtc taaatcttca
    33961 aaaaagcgga tctcgttcac agattgatgg aaagctatcc attcgcaaac ttgtttggca
    34021 tgaaggaaag attgcggtgt ctcttgctct gtataacaaa gcgacccaat tccaaagttt
    34081 agctgtaact gatacatttt attaaacttt gtctggattt tatcaagcaa tgatagcaat
    34141 gaggctctgg aaatccgatt gactaggata atgatttgtt gtctatgtac agtgagcaaa
    34201 gcctgtccgt aataagagaa gaccctttcg agcaactcca ataaaggatt gatccgttct
    34261 tgacacaatt gctgattgtc agaaagttgt cccacaacta cataatatgg agaagaaaaa
    34321 tcaagctcaa ttaagtgagg gtggtatcga ctatgtactt gatatcatca atgaaccgat
    34381 ggtactttct tatgcgatgg aacaggtaga gcgattgctt gtttgtgcag gaaaacaagc
    34441 gatgcggctg atacgattag gtgagaaaat aagaagataa aaatacacgg tcccttcatg
    34501 gcgaccgtgc atttttggtt tttattaaat atttaatact ttattcaaga aatcttttgt
    34561 ccgttcattt tgcggatttt caaagatttg ttgaggcgtt ccatcttcta aaaagtttcc
    34621 gtcgtcaatg aacatgacgc gattggccac ttctttagcg aaccccattt cgtgcgtaac
    34681 gataaccatc gtcatacctt gttctgctaa ctttttcatg acgttcaaga catcaccgac
    34741 catttcagga tcgagtgcag aagtaggctc atcaaaaagc atgatgtcag ggttcatcgc
    34801 tagagcacga gcaatagcca cacgctgttt ttgtccacca gaaagcattt caggatacat
    34861 atctttttta tcttctagac caactgtctg cagtaatttg attgcttttt tctctgcttc
    34921 ttctctagat tctttcttca ggtccatcgg tgctaaaatg atgttttcca aaatagaaag
    34981 atgagggaat aggttaaagt gttggaatac cattccgata tgttgtctca ccaagttgat
    35041 atctgtgctt ttatccatca agtgggcgcc gtcaataata atttcaccgt cagaaggttc
    35101 ttctaaacga ttcaagcaac gcaagaatgt actttttcct gaacctgaag ggccaatgat
    35161 acagacgaca tctccttcgt tgatggatac attgatgtcg tttaatactg tgttatcgcc
    35221 gtattttttg actaaatgct caactaagat tttttctgcc atcttacttc accttctttt
    35281 ctaaaacttt tgctaatttt gtcaatgctg tgatcaaaat caagtacatg atcgcaatga
    35341 tcaagtaaac atacgtactt tgtgttgtac gtgcaacaat gatcttacca gtttgcagca
    35401 attcgactac accgattgcg gaaatgatcg ttgtgtcttt taatgagata acgaactgat
    35461 tgacgaatga tgggatcatg atgcgtatcg cttgcggcaa aatgattttt tgcattgtcc
    35521 gattataaga aagacctaaa cttctggaag cttccatttg tccgacagga acggcgttga
    35581 tcccgcctcg aacaatctct gcgatataag cacttgcgtt aagagtcaag gtgataacac
    35641 cagcaaggaa gtcaggaatc gtaaatccaa cgataccagg caacccaaag aaaataaaga
    35701 acgcaagaac catcattgga attcctcgga tgatatcgac ataaatacta gcaattgtgc
    35761 gaagagcgcg tattggtgct acactgaata atccaaaaat cacaccgatt actatagcca
    35821 aagcaaagga gatcaaagtt aggagaattg ttctccaaag tcctttcaac aatgcttgat
    35881 agttgttttg gataagtcca agaaatgttg attcatcagc agtggaagac tcactggaag
    35941 tttctgaacc atccgctaca tattctttaa taatcttatc gtattcgcct gttcttttta
    36001 attctttcaa tccttcattg aacatattaa gaagctcagg attttgtcct tttttaacgg
    36061 caaatgcata ttttccgcca gattcttttt tgatcggtgt ttccaattct tgtccttgtt
    36121 tgatcgcata accgattacc ggataatcat ccatcattgc atcgattgcg ccaactttta
    36181 acccgtcgta caattgatca gcagcatcaa aatatttgat agaatagcca tattcagatt
    36241 catggtcgct taaaaattca gcactttcgg taccaacttt tgctccgact gtttttcctt
    36301 tcaaatcttc ataagacttg atcgaagtat ctcctttttt gacagctaat tggatcccgc
    36361 tatcaaagta agaatcagaa aaatcatagc ttttctttcg ttcatctgtt acagtcattc
    36421 cggcaatcat tccgtctgct tgtcctgatt ccaaagcttg caaagcagca gagaaaccac
    36481 ggtagtccat tcggatgtta aatccttgca gtttggcgat gttttccatc aattttacat
    36541 cgatgccttc gtactcgccc tttgcgtttt gaaattcaaa tggagcaaaa gcagagtcac
    36601 tggcaattac gtatacgtct tttttgggct ctattttttg cattgtctcg cttgtgcttg
    36661 actctgccgt tgtagtgttg tcagcttgtg caacatgacc aaacgcaaat gtcacaagtc
    36721 ctgtcattaa gaaaacaaat gagtgaaata aatgctttct tttcataatc aacctcctaa
    36781 atttccaata ttttattatt ataggcgaaa agtgctgaat cagacaagac tgtaagctcg
    36841 aaaaaatgaa aaaaatgcga gtttctaaca ttttatgtta taaaacataa catgtataat
    36901 aagataaaaa agtggcgaaa gtatgttcgg tgtggtataa ttttttagta aggaatgcct
    36961 ttgctttcct tttcttttta ttggtcgtaa aatgaaaagg aatgaatgaa aaggaggacg
    37021 aagaatgatt gagagagata cctcccgaca ctttgagctt gtctcgaaat accaaccggc
    37081 tggtgatcag cctgaagcga ttaaccaatt agtggatggc gtagtcggcg ggaaaaaagc
    37141 acaaatctta ttaggtgcga ctggaacagg gaaaacatat acgatttcca atcttattga
    37201 gaaagtgaat aaaccaacac tgattattgc ccataacaaa acattggctg gtcagctgta
    37261 cggggaattc aaggaatttt tccctaacaa tgctgttgaa tattttgttt cttattatga
    37321 ttattatcag cctgaagctt atgtgccttc aagcgatacg tatatcgaaa aagattcaag
    37381 tgtaaatgat gagatcgaca aattacgaca ttcggctact agttcattat tagaaagaaa
    37441 tgatgtgatc gtcattgcat ccgtttcatg tatttttgga ttaggctcgc cttttgagta
    37501 ccaaaaacaa gtagtttcga ttcgacaggg agcagaacta gatcgcaatc agttgattcg
    37561 tgacttagtc agtatccaat tcgaaagaaa tgatatagat tttcagcgcg gtcgttttcg
    37621 agtgcgcggt gatgtcgtgg agatttttcc ggcttcaaga gatgaacgtg ccttacgtgt
    37681 ggagtttttt ggagatgagg tcgaacgtat ccgagaagtc aatgcactga caggtgaagt
    37741 tttaggagaa acggaacacg tagcaatctt cccggcgact cactttgtga cgaatgatga
    37801 acatatggag catgccgtag cgaatatcaa agcggaatta gaacagcgtt taactgtctt
    37861 gcgaaatgaa aacaaacttc tggaagctca acgtttagaa cagcgaacca attacgatat
    37921 tgaaatgatg ttagaaatgg gctacacgtc gggaattgaa aattattctc ggcatatgga
    37981 cggacgtaaa gaaggtgaac cgccttatac gctattggat ttctttcctg aagatttttt
    38041 gatcgtggcg gacgaatcgc acgtaaccat gcctcaaatc agaggaatgt ataatggtga
    38101 ccgagcgaga aaacagatgc tagtagatta cggtttccgt ttgccatctg ctttagacaa
    38161 tcggccactt cggttagaag agtttgaaaa acatgtcaat cagatcattt atgtctctgc
    38221 gacgccagga ccatatgaac atgaacaaac agataccgtt atccaacaaa tcatccgtcc
    38281 gacagggctg cttgacccag tgatcgaagt tcgtccaatc atgggacaaa tcgatgatct
    38341 agtaggcgaa ataaatgaac gagtcgaaaa agaccagcgg gtgtttgtaa cgacattgac
    38401 gaaaaaaatg gcagaagacc tcacagatta tttcaaagaa ttagggatca aagtcaaata
    38461 tttgcatagt gatatcaaaa cgctggaacg aacagaaatc attcgagatc ttcgattagg
    38521 tgaatttgat gtgttagttg gaatcaattt gcttcgtgaa ggattagacg tacctgaagt
    38581 ttcccttgtt gctatcttgg atgcggataa agaaggcttt ttacgtagtg aacgttcctt
    38641 agtccaaacg attggacgag ctgctcggaa tgaagaaggt aaagtaatca tgtacgcaga
    38701 caaagtaaca gattctatgc gtctagcgat ggatgaaacg tcaagacgtc gtacgattca
    38761 gcagaagtac aatgaagaac atgggatcgt tccaaaaacg atcattaaag aaatccgaga
    38821 tttgatttct attacgaaag aatcggaaga tgatacaaaa gaagcggtac aagtctcgta
    38881 cgaagaaatg acgaaggaag aaaaagacac gttattgatg aaattagaaa aagaaatgaa
    38941 agatgcggca aaagctttgg attttgaaac agccgcgaat gtgcgagata tgattttaga
    39001 attaaaagct tcaaagtagg aggacaacat ggctaacgat aaaattacga ttcatggtgc
    39061 acgtgcccat aacttgaaaa atatagatgt aacgattcca cgagataaat tcgtggtcgt
    39121 tacaggtttg tccggttctg gaaaaagttc cctagcattt gatacgctgt acgctgaagg
    39181 gcagcggaga tacgtagaga gcctttctgc ttatgctcgt cagtttttag gacaaatgga
    39241 taagccggac gtagatagca tcgacggatt gagtccggcg atttctattg atcagaaaac
    39301 aacaagtaaa aatcctcggt cgacagtagg tactgtgacg gaaatcaatg actatttacg
    39361 cttgttgttt gcacgtgtag gacatccgat ttgtcccaac gatcatatcg agattaccag
    39421 tcaatcagta gatcaaatgg cagatcaagt aatggctttg cctgagcgca caaagatcca
    39481 agtgttggca ccgatcgtgg taaagaaaaa aggacaacat aaaaaaatat tcgaacgaat
    39541 ccaaaaagag ggatacgtcc gcgttcgagt cgatggtgag atctatgatt taagtgaagt
    39601 gcctgagctg gagaaaaaca aaaaacatga tatcgcgatc gtcatcgacc ggatcattgt
    39661 gaaagaaggg attcgttctc gcctatttga ttctttagag gcggccctgc gattagcaga
    39721 cggctatgct ctggttgacg tgatcggtga agaagaaatg ttgtttagcg aacactatgc
    39781 ctgtccttat tgcggattta ctgttggcga actagaacca agactgttct cattcaatgc
    39841 gccttttgga gcttgtccag actgtgatgg tttaggtgtc aaattagaag tcgatcaaga
    39901 tttagtgatt ccagatagaa cgaagacact tagtcaaggt gctatcgttc catggaatcc
    39961 aatcagctct cagtattatc ctcaaatgtt agcgcaggct tgtgaaagtt tcggtatcga
    40021 tttagataca ccattcgaag aattaccgga agagcatcag gagatcatct tgaatgggac
    40081 agatgatctt ttccatttcc attatgaaaa tgattttggt ggcgttcgcg atgtagaaac
    40141 gactttcgaa ggggtattga agaatatcga gcgtcgttat catgatacga acagtgattt
    40201 cacgcgtgag caaatgcgtc tttatatgac agaattaact tgtcagacat gtaaaggtta
    40261 tcgattgaat ccccaagctc tatctgttca aatcaatgga acgaatatcg ggcaaacaaa
    40321 tgagttgtct attcaaaaag cagtagattt cttctcaaac ttaacgttat ctgaacaaga
    40381 gaaagtaatt gccaaaccga ttttaaaaga agtaaatgac agactttcat tcttggaaaa
    40441 tgtgggacta gactatctaa ctttgagtcg cgcttccgga acattatctg ggggagaagc
    40501 gcagcgaatc agattggcta cacaaattgg atcgaatctt tctggagttt tgtatatttt
    40561 agatgaacca tcgatcggat tgcatcaacg cgacaacgat cgactgattg gctcattaaa
    40621 gaaaatgcgt gatcttggca acaccctcgt tgtagtcgaa catgatgaag atacgatgtg
    40681 ggcagcagat tatttgattg acgtcggacc tggggccgga gaacaaggcg gacaaatcgt
    40741 ggctgcaggc actcctgaag aagtagctaa tgatgaaaat tctttgacag ggaaatatct
    40801 ttcagggaaa aaatcaatcc ctgtaccaaa agaacgccgt aaaggtaatg ggaaagcaat
    40861 caagatcact ggtgcttcag aaaacaatct gaaaaatatt agtgttgaat ttccattagg
    40921 agaatttgta gcagtcacag gtgtgtctgg ttctggaaag agtacattgg tcaatagtat
    40981 cttaaagaaa tcgttagcgc aaaaattaaa taagaattct gctaagccag gtaaattcaa
    41041 gacaatttcc ggctacgaaa gtatcgaaaa gatcatcgat atcgatcaaa gcccaatcgg
    41101 ccggacgccg agaagtaatc cagcgactta tacaagtgta tttgatgata tccgtgggtt
    41161 atttgctcaa acgaacgagg caaaaatgcg gggttataag aaagggcgtt ttagtttcaa
    41221 cgtaaaaggc ggtcgttgtg aagcttgtcg cggggatgga attattaaga tcgaaatgca
    41281 ctttttgcct gatgtctatg ttccttgtga agtatgtcat ggtaaacgat ataattctga
    41341 aacattagaa gtgcattaca aaggaaaaag cattgctgat attttggaaa tgacagtaga
    41401 ggatgctgta gaattcttca agcacattcc aaagattcat cgcaaactgc aaacgattgt
    41461 tgatgttggc ttaggttatg tgacgatggg gcaaccagca acgacattgt ccggtggtga
    41521 ggcacaacgg atgaaacttg ccagtgaatt gcacaaaatc tctaatggaa agaatttcta
    41581 tatactagat gaaccaacga caggacttca tagcgatgac atcgcccgct tgttgcatgt
    41641 attacaaaga ttagtagatg ctggtaacac agttttagtg attgaacaca atctagatgt
    41701 aatcaaaaca gcagattata tcattgattt aggaccagaa ggtggagaag gtggaggaac
    41761 gatccttgcg actggaacac cagaagaaat cattaacgta aaagaaagtt atacaggtca
    41821 ctatttgaaa aaaataatgg tataaagagg atagatagct gagatagtta aaaaaacata
    41881 aaaaaatgaa tttttgcttg ttttattaat atcagtgttc tagaatcatt acatggtagt
    41941 tctttctaca ctgattggta tagataaaaa cttcctataa gttcaatccg ttcaaaaaag
    42001 aattgagcaa tcggaggact ttcctttgat tgcttttttc tttatggtat gatattcctt
    42061 gtgtagttgg aaaagaaaaa caagagatta tgagtaagtg agagataatg atgaacagta
    42121 ttgaaaatga agaatttatc cctatattat taggaagcga catgaacgtt tatggtatgg
    42181 cacgttcttt taatgaagca tacggcaaaa tctgccaagc atatgcttcg gatcagttag
    42241 cccctacccg ctacagtaaa attgtcaatg tagaagttat tccaggattt gataaagacc
    42301 ctgtatttat cgagacgatg cttcgtttgg caaaagaaag atattctgat aagagtaaaa
    42361 aatatctttt gattgcttgt ggtgatggat atgcagaatt gatctcccaa cataaacaag
    42421 agctgtcaga atattttatc tgtccatata ttgattattc tttatttgaa aggctgatca
    42481 ataaagtaag cttttatgaa gtttgtgaag aatatgatct tccgtatcct aaaacattga
    42541 tcgtcagaga agaaatgtta gtaaatggac atttggaaca ggaattgcct tttgaattcc
    42601 cagttgcttt gaaacctgca aacagtgtgg agtatctttc tgtccaattt gaaggaagaa
    42661 aaaaagcttt tattttagag actagagaag agttcgattt aattttaggc cggatatacg
    42721 aagccggtta taaaagtgaa atgattgtac aagattttat cccgggtgat gatagtaata
    42781 tgcgtgtgct aaatgcgtat gtagatgaag atcatcaagt tcggatgatg tgccttggtc
    42841 atccactatt agaagatcct acaccagcat cgattggtaa ttacgttgtg atcatgccag
    42901 attataacga aaaaatctat caaaccatta aggcattttt ggaaaaaatc gaatatacag
    42961 gctttgccaa tttcgatatg aagtatgatc ctagagatgg agaatataaa ttatttgaaa
    43021 tcaatttgag acaaggtcgt agcagtttct ttgtgacatt gaacggcttg aatctggctc
    43081 gttttgtcac agaagatcga gtgttcaaca aaccctttgt tgaaacgacc tatggaacga
    43141 atcaatcaga taaagcaaga ttatggatgg gtgtgcccaa aaaaatcttt ttagaatatg
    43201 ctcgggaaaa tgaagacaaa aaactagctg agcaaatgat caaagaaaat cgttacggta
    43261 ctactgtatt ttatgaaaaa gaccgatcaa tcaagcgctg gttgttgatg aaatatatgt
    43321 tccataacta tattccaaga ttcaaaaagt attttcatgt gaaagaaggc tagttcatgg
    43381 agaatttttt cagtatttta ggcggaatgg gcacgatggc gacagaaagt tttgttcgct
    43441 tgatcaacca ccgaacgaaa gctacaaaag atcaagaata tttgaattat gtcttattca
    43501 atcatgcaac agttcctgat cgtacagctt atattttaga tcgatcagaa gaaaatccaa
    43561 tgccgtttct gctggatgat attgagaaac aaaatttatt gcgaccaaac ttcattgtgt
    43621 tgacttgtaa caccgcacac tacttttttg aagagttaca agcggcaacg gatattccta
    43681 tcttgcatat gccaagagaa gcagcaaatg aacttgtccg tcagcatacg acagggagag
    43741 tagcgattct aggaacagaa ggaagtatga aagctggaat ctatgaacgc gaagtcaaaa
    43801 atcttggttt cgaaacagtg attcctgata ctgcgcttca agaaaaaatc aattacctga
    43861 tctatcatga gatcaaggaa tcggattatt tgaatcaaga gctctattac gaaatcttag
    43921 aggaagcggt agagcgccta aactgtgaaa aagtcatcct aggttgcaca gaattatctt
    43981 tgatgcatga atttgctgaa gataatcatt acccagtgat cgatgctcaa tctattttag
    44041 cggatcgaac gatcgaacgt gcattagctg aaagaagcga ggcattggat acagcatcgg
    44101 aaaagtaaaa aaattggatt tggggcaaat atcctaaaag atatttggtc caaatctttt
    44161 tgtaaaagaa aaagaaaact ggtaatgata cgcccgggat gatataattt tattatgcac
    44221 aatgtgagag gggttcgtaa atatgacaga taatttgcaa ttagtcatca taacaggtat
    44281 gagtggtgca gggaaaacag tagctatcca aagttttgaa gatatgggct atttttgtat
    44341 cgataatatg ccgcctcgat tgattccaaa attctgggaa ttgatcaaag aatcaggtaa
    44401 agtcacaaaa atggctttag gagtggattt gcgttctcgt tccttctttg aagaaatcca
    44461 aaacatgctg atcgaaatcg aaaatacgaa cttcatcgac acttctatct tatttttaga
    44521 tgcttcagac gaagaactag tggctcgcta caaagaaaca agacgagcac atccaatggc
    44581 gatggacggt ttgatcactg aagggatacg taaagaacgg gcgatactag acgacttgaa
    44641 agcccaagct tccgtgatca ttgatacgac aacattgaca ccacgacaat tacgtgaaaa
    44701 aatcaatgag tcattcaaat caaagggaga ccaaggattt cggatcgaat ttgtatcatt
    44761 tggttttaaa tacgggttgc cgatcgattc ggatattgtg atggatgtgc gttttttgcc
    44821 caaccctcat tatatcccgg aactaagacc attgaccgga aaagatcagc cagtctatga
    44881 ttatgtgatg tcctttgatg aaacgcaatc attctaccat aaatttttgg atcttctgga
    44941 aactgtattg cctggataca ttaaagaagg aaaaagcagc ttgacagttg caatcggctg
    45001 tacaggcggt caacaccgtt ctgtggcatt gactgaacgt ttggcaaatg ctttagggaa
    45061 aaactataaa gtcaatgtga cacatcggga caaggataaa aggaaagaga cggtgaaccg
    45121 gtcgtgaaaa tgaaaaccta ccggataaga aaaccaaaga tcgtagtggt cggcggtggc
    45181 actggtctgc cagttatcct gaaaagttta cgaaatcaag gagcagatat cacagcagta
    45241 gtgactgttg cagatgatgg tggaagcagc ggtgcaatca gagaatcgat cgctatggct
    45301 cctcctggtg acttgagaaa tgtcttagtg gcgttatcgg atatgccaca attctatgaa
    45361 gatattttcc aataccgttt caaaaaggaa gatcagtttt tagccaatca tacgatcggt
    45421 aatctgatca ttgccgcagt ttccgagatg cggggaagca cttatgaggc gattcaatta
    45481 ttagcaaaaa tgatgcatgt agatggacat gtttatcctt cttcagaaac tcctttgacc
    45541 ttgcatgctg tttttaaaga cggaacgact gcagtaggag aatctaaaat cgcgatcaac
    45601 cgtaaaacca ttgaccgtgt gtatgtctgc aacacaaatg ataaaagtga agccaaagcg
    45661 gctcgaaaag tcgtttcagc aattatggac gccgacatgg tcgttttggg acccggaagt
    45721 ttattcacca gtatccttcc aaatttagtg ataccagaaa tcggcgaagc aatgctcaag
    45781 acaactgctg aaaccgtcta tatctgtaat atcatgaccc aaaaagggga aacggaacat
    45841 ttcacagatg ccgatcatat acgtgtttta cacaagcatt tgaatcagcc ttttatcgat
    45901 acagtattgg tcaatacgga aaaagtgcct gaagattata tggacccaga gatctatgat
    45961 gagtatctag tacaagtcgc acacgacttc aacggattga gaaatgaagg atgtcgagtg
    46021 atttccaccg actttttaga attgaaagat ggtggtgtct tccacgatgg agacaaagta
    46081 gtagaagaat tgttccgttt agttttcggt tcaaaatatt aagcaattta ttgttggaga
    46141 aaggaggaga ataggatgtc atttgctgca gatgtgaaga aagaactaac tggtttggaa
    46201 gttcatcgag aacatgcgaa agcggaatta gctgctttgc tgcgaatgaa cggctcttta
    46261 ggtattgtga accagcagtt tattttgaac gtccaaacag aaaatgctgc aattgccaga
    46321 cgtatctatt ctcttttgaa agatcattat caggtacgaa gtgagctact cgttcgtaaa
    46381 aaaatgaaat taaaaaagaa caatgtctat atcgtccggt tgaagcaagg cacaaaagat
    46441 gtgttgatgg atcttgatat catggatggt atgatgttca atggacacgt ttcggacgag
    46501 attatgggaa attcgcaaaa aatgcgttct tacttacgtg gtgcttttat ggcctcaggt
    46561 tcagtcaata atccggaaac gagtcgttat catttggaaa tattttctat ttatgaacag
    46621 cataaccaag acatttgcga tatgttaaat tattacggtc tacatgctcg aacgcttgaa
    46681 cggcgcaatg gctatatttc gtatttaaaa ggagcagaaa aaattgctga tttcttaaca
    46741 ttgattggtg caaccaactc aatgctccga tttgaagatg ttcgaatcgt acgggatatg
    46801 agaaattcgg tcaatcgtct agtcaattgt gagacagcaa atctgaacaa gacgattgat
    46861 gcggcttcta aacaaatcga aaacatcgaa ttgatcgaag caaaggtcgg tctacatgcc
    46921 cttcctgaaa aacttcagga aatcgcagaa cttcggcttc aacatccaga ggtcagcttg
    46981 aaagaattag gggaaatgat tccctcaggt gcaatatcaa aatcgggaat taatcaccga
    47041 attcgcaaaa taaatgaatt tgcggaaaaa ctgcgtgaag aagcagttta atccaaagaa
    47101 aaaactgata catgaagcag gagatgcgaa tgtatcagtt ttttttaaaa gctatacttg
    47161 cttttcttcc gtcaaactat aattccaact ttactggagt aacataatgt tgaaaggctt
    47221 ctattgtttt ttaatacaat cgtataaaaa gcggggagac aactgaatga agattttagt
    47281 tttatacata aacttttgct taatatagaa gtcctgatat agataggatc aagtatttat
    47341 ctataactgg agattaatta aaaactggaa ggaactgctc gatcaacctt gagaactgtt
    47401 tttcttactt ctggtcaaga ttctcgtggt tggtggataa ggatggttga taagttcatt
    47461 ggattagcta gtcgaaaaca accaggtgta attaatgtag aagttttaac aaggggatgg
    47521 agtgatttat tcagtgataa tcataagttc ataaatagta attgggatgt atggttggta
    47581 tacgttatac ctgaacttga actatcaaga tttggtttag aatttgagaa tcgatgttta
    47641 taaagggagg gcaaaaaatg tgctttttta caacttttac tatcattatg attgcagtac
    47701 tttttatttt tctagatata gcaaagcgaa atacagcatt tttattatat cgggttttac
    47761 ttcgagcagg tctcataaca tttatatcga tcgttggatt ttttctattc acagttattg
    47821 tatttatttg gagaactaca gcaccaccat tgccagagat tacatatgga gaatttccgt
    47881 ttagtcttga atatgaaata gatgaggaaa gaattttgat tgaagatacg gtgattgcag
    47941 aatttatagg aagttttaga gggaatgtga caactcaacc tagacgaagt tggagtataa
    48001 aacttttaaa taatagtgga gagttgaaag catttcatta tgaattctta ataatagaaa
    48061 gtgaagatgt aacaatcact ttaagtagtg gctctgctgg atattttatg aatgatggtg
    48121 gaagcaagcc acagctcaca gtccgtgatc gtattgcagg ttttatgcat ttttctcact
    48181 atgacttaga agaagctcgt aaaatacttg cagaatatgg gatcacatta atcaattggg
    48241 aatatgcgcc accgataaga aattttttca atgattatta ttagtcttat aaaaaacaga
    48301 atgtaaaatt tatgtgcgcg agtatttagt tttccatttt ttgtttgata gatatatagc
    48361 attgttgaag catgggatag tggtgccaat tttatcaatg aggttggtca ggcagttaac
    48421 ataattggtg atgctataaa tccatttaat tggaattgga ggaattaggt gtggaaacag
    48481 tgagattatg gatattcatg aaaaattttc gttcggcttt gagatatgtt attctggaat
    48541 caaaatcagg ttattatctg attgacacag atcactatac tttttgggga ggctaccttt
    48601 ttccgatttt taattggata atcccccata aggttatgaa aattaacttg cctgcagaag
    48661 aaataaatag attaatactc aacaaagatt tacaaaggaa aagagagaaa gaacattcta
    48721 atgttgcttg gataggtcca gcatttgcag tagtgacttc ttcaattgtc ggtcctatgc
    48781 tagagagtat ttatagtgat ttttctattg gaatacgtta tttatttgtt actttaagta
    48841 tagctgttct tttgtgcata aagggttatg ttagcaagaa gaataaagat attttaacac
    48901 taatcggtgt aaacaatt
//
LOCUS       DARDHA010000006        45125 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000006 DARDHA010000000
VERSION     DARDHA010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45125
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.006"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            71..1129
                     /locus_tag="SDA10_000269"
     CDS             71..1129
                     /locus_tag="SDA10_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEG4239202.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            1133..2167
                     /locus_tag="SDA10_000270"
     CDS             1133..2167
                     /locus_tag="SDA10_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEG4239203.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(2269..2985)
                     /locus_tag="SDA10_000271"
     CDS             complement(2269..2985)
                     /locus_tag="SDA10_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG4239204.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(2954..3739)
                     /locus_tag="SDA10_000272"
     CDS             complement(2954..3739)
                     /locus_tag="SDA10_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEG4239205.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3730..4479)
                     /locus_tag="SDA10_000273"
     CDS             complement(3730..4479)
                     /locus_tag="SDA10_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG4239206.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4777..5301
                     /locus_tag="SDA10_000274"
     CDS             4777..5301
                     /locus_tag="SDA10_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEG4239207.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5317..6816
                     /locus_tag="SDA10_000275"
     CDS             5317..6816
                     /locus_tag="SDA10_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEG4239208.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(6904..7506)
                     /locus_tag="SDA10_000276"
     CDS             complement(6904..7506)
                     /locus_tag="SDA10_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG4239209.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7610..8566
                     /locus_tag="SDA10_000277"
     CDS             7610..8566
                     /locus_tag="SDA10_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEG4239210.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8871..9827)
                     /locus_tag="SDA10_000278"
     CDS             complement(8871..9827)
                     /locus_tag="SDA10_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG4239211.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            10001..10561
                     /locus_tag="SDA10_000279"
     CDS             10001..10561
                     /locus_tag="SDA10_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239212.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10554..11378
                     /locus_tag="SDA10_000280"
     CDS             10554..11378
                     /locus_tag="SDA10_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEG4239213.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11388..12005
                     /locus_tag="SDA10_000281"
     CDS             11388..12005
                     /locus_tag="SDA10_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEG4239214.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            12023..12706
                     /locus_tag="SDA10_000282"
     CDS             12023..12706
                     /locus_tag="SDA10_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEG4239215.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13389..14624
                     /locus_tag="SDA10_000283"
                     /pseudo
     CDS             13389..14624
                     /locus_tag="SDA10_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            14632..15519
                     /locus_tag="SDA10_000284"
     CDS             14632..15519
                     /locus_tag="SDA10_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4239216.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15536..16372
                     /locus_tag="SDA10_000285"
     CDS             15536..16372
                     /locus_tag="SDA10_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4239217.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16375..16998
                     /locus_tag="SDA10_000286"
     CDS             16375..16998
                     /locus_tag="SDA10_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239218.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            16995..18287
                     /locus_tag="SDA10_000287"
     CDS             16995..18287
                     /locus_tag="SDA10_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG4239219.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18646..18743
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18859..19440
                     /locus_tag="SDA10_000288"
     CDS             18859..19440
                     /locus_tag="SDA10_000288"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEG4239220.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19443..20750
                     /locus_tag="SDA10_000289"
     CDS             19443..20750
                     /locus_tag="SDA10_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEG4239221.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21043..21531
                     /gene="purE"
                     /locus_tag="SDA10_000290"
     CDS             21043..21531
                     /gene="purE"
                     /locus_tag="SDA10_000290"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEG4239222.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21524..22648
                     /gene="purK"
                     /locus_tag="SDA10_000291"
     CDS             21524..22648
                     /gene="purK"
                     /locus_tag="SDA10_000291"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEG4239223.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22661..23956
                     /gene="purB"
                     /locus_tag="SDA10_000292"
     CDS             22661..23956
                     /gene="purB"
                     /locus_tag="SDA10_000292"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEG4239224.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24136..24513)
                     /locus_tag="SDA10_000293"
     CDS             complement(24136..24513)
                     /locus_tag="SDA10_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239225.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            24769..25497
                     /locus_tag="SDA10_000294"
     CDS             24769..25497
                     /locus_tag="SDA10_000294"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEG4239226.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            25497..25751
                     /gene="purS"
                     /locus_tag="SDA10_000295"
     CDS             25497..25751
                     /gene="purS"
                     /locus_tag="SDA10_000295"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEG4239227.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            25753..26424
                     /gene="purQ"
                     /locus_tag="SDA10_000296"
     CDS             25753..26424
                     /gene="purQ"
                     /locus_tag="SDA10_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEG4239228.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            26425..28647
                     /gene="purL"
                     /locus_tag="SDA10_000297"
     CDS             26425..28647
                     /gene="purL"
                     /locus_tag="SDA10_000297"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEG4239229.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            28632..30071
                     /locus_tag="SDA10_000298"
     CDS             28632..30071
                     /locus_tag="SDA10_000298"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEG4239230.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            30094..31146
                     /locus_tag="SDA10_000299"
     CDS             30094..31146
                     /locus_tag="SDA10_000299"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEG4239231.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            31143..31721
                     /gene="purN"
                     /locus_tag="SDA10_000300"
     CDS             31143..31721
                     /gene="purN"
                     /locus_tag="SDA10_000300"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEG4239232.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            31718..33253
                     /gene="purH"
                     /locus_tag="SDA10_000301"
     CDS             31718..33253
                     /gene="purH"
                     /locus_tag="SDA10_000301"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEG4239233.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            33269..34543
                     /gene="purD"
                     /locus_tag="SDA10_000302"
     CDS             33269..34543
                     /gene="purD"
                     /locus_tag="SDA10_000302"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEG4239234.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            34665..35579
                     /locus_tag="SDA10_000303"
     CDS             34665..35579
                     /locus_tag="SDA10_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG4239235.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(35712..37277)
                     /locus_tag="SDA10_000304"
     CDS             complement(35712..37277)
                     /locus_tag="SDA10_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG4239236.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(37270..38721)
                     /locus_tag="SDA10_000305"
     CDS             complement(37270..38721)
                     /locus_tag="SDA10_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG4239237.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(38735..39751)
                     /locus_tag="SDA10_000306"
     CDS             complement(38735..39751)
                     /locus_tag="SDA10_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4239238.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            39971..41059
                     /locus_tag="SDA10_000307"
     CDS             39971..41059
                     /locus_tag="SDA10_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEG4239239.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            41113..42639
                     /locus_tag="SDA10_000308"
     CDS             41113..42639
                     /locus_tag="SDA10_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEG4239240.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            42641..43846
                     /locus_tag="SDA10_000309"
     CDS             42641..43846
                     /locus_tag="SDA10_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4239241.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            44103..45080
                     /gene="guaC"
                     /locus_tag="SDA10_000310"
     CDS             44103..45080
                     /gene="guaC"
                     /locus_tag="SDA10_000310"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEG4239242.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
BASE COUNT        14301 a         7769 c         9969 g        13086 t
ORIGIN      
        1 tgttaatacg tattagcaaa ataaaagctt tttttattgt gaataaatga acataattag
       61 gaggagaaag atgaacttat ttgacatttt tcgatcgact ttgacagata tgtcgattgt
      121 cagtgcaatc acttcaacag tttttattat attattaggg tttgtttgta gaaaaaaggg
      181 gattttttct gaatctgttg gaaaaattct atcgaaagtc gtcttaacag tggctttgcc
      241 agcactggca tttcgagctt tcatgcaaga tatcaataca gaattactga tgcaaggaat
      301 caatgtcttg atttggggat tagttattta tattattctt attctagttt caaaaccttt
      361 tttcttaagc tataaagggg ataagcaaac aacgttgcgt gtcctgacga tctttggttc
      421 tacgaccttt ttcggaacgc ctatcgtcag tgcgatttat ggtcctgtcg gcgtgatgta
      481 ttcctcaatt ttcaatattg gctaccgaat ctttttgtat tcttatggat acataaaaat
      541 gagtggcttg aagatggaag ctaaaaatat caaagcgatg ttcgtcaatc caatcattat
      601 cgctactttt gccggccttt tcatttggct gttccaaggg tatctgccac aagtgtcagt
      661 tacaggtgca gaaggagaga taagccaagt agcgtttttg cgaattgacc agactgcacc
      721 gtggctattt cagccgatgg attatttagc tggtttaagt tcaccattgg cgtggctagc
      781 aatcggcgca acattaggtg aagtaagctt caaaaaagct gcatctgaaa aaacttcatg
      841 gtattacagt ctagtcaaag tggtatttgt acctgcattg aatatcgtga ttctagcagt
      901 tttgacggca acgaatattc taccagtcaa ttttgaagca ctggcaacaa tcgttatcat
      961 gatggctaca ccagctgcaa cagtggcagc agcctatgct atcagttttg atagggaggc
     1021 acttttagcc tccaatgcat ccttactgtc aactattttt gctgtggtac tgacacctgt
     1081 atggatcgtt gtattacaaa tgattcatgg cataggttta ttttcataag aaatggagga
     1141 agaaagtatg agaatcgcat gttacggtgt tcgtccgaac gagatagatt tttttgaaaa
     1201 attgaatatt taccattatg atctcagtct ttatgaagaa ctgttgacac atgacaatat
     1261 cgtgtcagca aaggaccatg atgctgtatt gttgagggga aattgtatag cagatgagac
     1321 caatcttgca aaaatgcagg aatatgggat tcgttatgtt tttacacgga ctgtgggagt
     1381 caatcatatc gatctacaag cggcagctga ttttggtatg acagtagccc gtgtaccatc
     1441 gtattcgcca aatgcgatag cagagttatc tttgactttt gccatgatgc ttttgagaaa
     1501 tactgcgtat acgacgatcc gaacatcttt caaagatttt cgagtggatg agcaaatgtt
     1561 tagtcgtgaa atcagaaatt gcacagtagg gatcatagga acaggcagga tagggttgac
     1621 ggaagctgtt ttgtttaagg gactaggagc caaagtgatc ggctacgata ttcatcagac
     1681 agaagctgct aaaaaagtat tgacgttcca ctcgcttgaa gagttattag ccaaaagcga
     1741 tattgtcagt attcatgtac ctcatatccc aggtgaaaac gatcaaatga tcaatgaagg
     1801 atttttagct caaatgaaaa aaggtagcat cttgattaat acagcccgtg gagagcttca
     1861 agataatgaa gctattttaa gagctttgaa aagcaatcat ttagctggtt ttgcaaccga
     1921 tgtttttgca aatgaaacag aggttttttt ccgatccttc aagccatggg agacgattcc
     1981 agaccctgct atccagcaat tagtagaaat gtaccctaga gttcttgtaa ccccgcatgt
     2041 aggttcaaat acagatgagg cattatcaaa tatgatttcg acaagtttcg agaactttcg
     2101 tgaaataata gaaactggaa aaacgaaaaa tgaggtatct cttcctaaag cccgtcaatt
     2161 aagataagaa aacatggata ttcgtcaata aaatgtttaa aaatagtcca agtaaacagt
     2221 tgttacttct gtatacttgg actattttgt atgggataat tcgatgactc aacagtaatc
     2281 tgtgagcttt ttccgcccat tctttgtcaa ttcataacag gttagcggct ggccgatttc
     2341 caaatatttg attctttcgt tcaatatatc ttgttctact aaaaaattca aatatttctt
     2401 gacggatata cgtgacagtg agcttttttg agccagttct tttgttgaaa aaatagtatc
     2461 ttcttttaga ataaatgcac ttattttttt taaagtcagt tttgacagac ctttaggcaa
     2521 atcgatgaat gatggtattt cgtgaatggg agtggtgtgg ttgaatactt gatcgatcaa
     2581 tttctgatcg gctttttcag tcgtttggat tgtctgagag aaagcaagat acttttcgat
     2641 cgccagctga aaacgttgca aagtgaacgg tttgatcaaa taatctaagg caccgtagcg
     2701 gatcacattc tttatggttt cgatatcatc tgcagcagtg atcaaaatca ctgggatatc
     2761 atttccttgc tgtctaagcc aagataaaaa atggatacca ctttcgccag gcatgtatac
     2821 atcaagcagc agcagatctg cgtttttttg tctgaaatat ttttttcctt cttctacatt
     2881 tctgaaactt tcgattacgt tcatattagg gatgctttcc acaaaagtac gattcaattc
     2941 tgcaaccatt ggatcatctt cgattatcag gacatttgtc atcattttga tcttcctttt
     3001 ttggtaattc tagatagacg attgtaccgc catttgtttg tttggaaaat tctaaaaatc
     3061 caccgtggcg tttgacgatt gaatggatga gattcaatcc aaacccacga ttgattccct
     3121 tcgtcgaaaa acctagcgta aatatttttt ctgagatttc ttctgatatc ccgacaccgc
     3181 catcgcagac ggtcacgatg aatacttcag cttctgtgtc atagtttaaa tgaatccgga
     3241 cctgtccgtt tctttcatgt ttttgtagca cggcttcttt tgcattatca agtaagttcc
     3301 ctaacacttg caaaagtgaa tggatggagt catccatctc cattttaggg ataaaagaat
     3361 cgctttctaa cagcatttca acagattgtt ctttggcttc gtttattttg cctaataaaa
     3421 aaccggcaat tgcaggtact ttgatcagac ttgagacttg gccaatctct tttttataat
     3481 tttgatttag ctgctggata aaatgggtga cttcttcata cttcttcaaa tcaatcaaac
     3541 cagaaatgac atgaagttta ttcatgaatt catgggtttg agctctcatg gagtttatat
     3601 attgtttcgt ccccccaagc tcctccatta atttcattac ttctgatcga tttcgtaaag
     3661 ttgcgactgc tcctaaaaaa cggtcattgc taaaaagagg tgtaacgcta gaaatagttt
     3721 ctatcccatt taaatacatg gattgagtcc gaacggtcgt ttgatgctca atcgtctctt
     3781 taaacaaaac gtcatataga ttatcactga tttttttcct acttcgaaat ccaatagctg
     3841 ctctgtttgg tagaaaatgt tttttgcagg tgtattgatc aagaggatag ttttatcaga
     3901 agagatggct aagattcctt catctacttc attttcgata atcgtttttt cacttaactg
     3961 ttctgctatt tcttgtggtt caagtccaag cagtactttt ttcatttttt gggcaagaaa
     4021 aacggcgcct atgatgccta cgctaagtcc aataatcaat gaaaaaacaa tcattttttg
     4081 tccatctttg acttgattgt caatcgtgtt gtttgttaag ccaacacaaa ctgcaccaat
     4141 aatctcttga tcataataaa tagggacgaa atagcggaag ccgaacccta acattccttc
     4201 tttttcagag aaaaattctt gtcctccaat cacttttttc acatcttcat gacttgagaa
     4261 aggaagaccg atgtaacttg ggtctgcgtg agaatatcga atgccttcag ggtccatgac
     4321 aacgataaaa tctaaattcg atgcaatcat gatttcattt gttattcctt gaatcgcatg
     4381 tgtcgggtca ccagtctgca gctttttttg gatgagttta tcattggcaa cgattttagc
     4441 aatgctagag attttatctg acacactaga tcggttcatc gaaagaataa aattcctgat
     4501 caatatggca gaaacaatca aagaaacgaa tgtggagaaa caaacgacaa ggatgatcgt
     4561 gttctggagc tttaatggtt ttttcttaaa aaaaggaaac ataggctacc tcgaaagaat
     4621 ttattgtgaa aattttaaca tgaaaacaaa aatttaacaa attgtcaacg ataaacacaa
     4681 ccttcctata aaaaacacca ctgagtcggt aaaagaagct atacagaatt ttcaaacatt
     4741 ggtatgatag acgtagaaat ggaaggaggc gtgtatatga tagggggaat cgcacttgag
     4801 acgatttatt ggtatacatt gctgatttgt gcaggattgg cagtcctatt gattattttt
     4861 ggagatgtat tcgattttga tggcccgctt gatccgatgc ttattatccc ttggttaact
     4921 ttcacaagct tatttggttt tctaggtgag cgtttaacag atcaatcatc tttattttta
     4981 tttattgcta gtggaggcat atcgatagtg ttggtatttt tgcttaattt ctatatattg
     5041 atgccgatga aacatgccga atcaacgatg tcgtcttctg aaaaaactct ggaaggacaa
     5101 gtagctgttg tgatcacacc gatccctgta cgaggtatgg gagaaattca gttgaaaagt
     5161 gttaccggtt ccattagtcg tccagcttgt ttttacagcc cccaagaaca gacagctgag
     5221 cggggaacga aagtattgat cattgagatc aaggaaagaa tctgttatgt catgccttat
     5281 gaaagcatga taaaagctta aaggagcgtg tgttaaatgg aaataccagt tgtttttatc
     5341 gcgatcgcaa tcgcagcttt gattttactg atgttgctga tcgtctttgt agcgaagtac
     5401 cagacagcaa aaccggatga agcgttgatc atcagtggaa gttatttagg aagcaaaaat
     5461 gtgcacattg atgaaagcaa taacaaaatc aaaattgtgc gtggaggtgg cgcattcgtc
     5521 ttgccagttt ttcaacgttc gaatcggatc agtctattat cgagtaaatt ggacgtctcc
     5581 acccctgaag tgtataccga acaaggcgta ccggtgatgt gtgatggtac ttcgatcatc
     5641 aaaatcggtt catctgtaga agagattgct acagcagcag aacagttttt aggcaagaca
     5701 agagaagaac tggaaaatga agcaagagaa gtattagaag gacacttgcg ctcgattctt
     5761 ggttctatga ctgtagaaga aatctatcaa aatcgcgata agttcagtca aagcgtacaa
     5821 gaagtcgctt ctgtagacct tgcaaaaatg gggttgatca tcgtttcttt cactatcaaa
     5881 gaggtcagag acaaaaacgg ttatcttgac tctttaggga aaccaagaat cgcccaagtc
     5941 aaaagagatg cagatatagc agaagcggaa gctctaaaag aaacgcgtat caaaaaagct
     6001 caagcagaaa aagaatcaca agcagctgaa ttacagcgac aaacagagat cgcagaatca
     6061 ttaaaagaaa aagaactgaa attagcaacc tacaaacaag aacaagatgt tgctaaagca
     6121 aaagcagacc aagcctacaa tttagaaagt gcccgagcac aacaacaagt aatcgaacag
     6181 gaaatgcaga ttaaaatcgt agaacgccaa aaacaaatcg aactggaaga aaaagaaatc
     6241 acacgtcgtg aaaagcaata tgattcggaa gtgaagaaaa aagcagatgc cgatcggtac
     6301 gctaaagaac aagaagcaca agctcaaaaa gtcaaagaag tgactgaagc cgaagcagaa
     6361 cgattccgtg tggaagcact ggcagaagcg gaagccaata agacacgttt agctggacaa
     6421 gcagaagccg aagcagcact agcaaaagga aaagcagaag ccgaagcaaa acaaaagatc
     6481 gccaatgcct tcaaggaata tggcgaagca gctgtcttga gtatggttat cgatatgcta
     6541 ccgcaattga tgcgcgaagc agcacagccg ctaggaaata tcgaaaaaat ctctgttgtc
     6601 gatacaggaa gcagttcagg agaaaccagt ggagcgaacc gagtgacaaa ttatgccaca
     6661 aatttgcttt cttctactca agaaacacta aaagcgacaa caggacttga cttgaaggaa
     6721 atcgtagaga atttgtcgac acgtgggact actagccaag taaatatgta tgaagcgcca
     6781 aaagaggcag aacaaaactc gatgaaggaa gaataaagta gtacacttca ccgttcaatc
     6841 aaaaatggag gaagactttt taaagttctt ctctccattt ttttatttta ttgcaaaaga
     6901 aaattagtcc tcgctatttt tcttttgttt ttggatctta gcttctatct cttgtaaatt
     6961 tgttcttagc aatcgttctt tcttttggag ttcgaccaaa tcttctgata gttcttcctt
     7021 cttttctgtt tcaggaggac gaagaaagaa attgataccg attgcaatga atgtccctat
     7081 aaaagtatct agtacacgtt ggatagcata caaagcggat tcgccttgag ggatgctcaa
     7141 cgcaatcagc aacaacgtag ctattgcgga aatgataccg gaattattgt taataccatc
     7201 tgacacgacg atcacaataa taactagaat aggaagcaag atcaattcta ccaaaaaatc
     7261 attgtgaaaa tagcttttga caaaaaaata aatgatagct aatgtaccac ctaatgtatt
     7321 gccgatgatc ctagactttc caaaagaaac ggttgtagtc aagtcctgac gcaaagaaaa
     7381 aacagcagat aatgcagcaa tcatcgggat tcctcgttga gtcaaatgaa aaaacaagat
     7441 acataaaaaa acagctaagg cacttttgat cgtacgcata cctagtcgaa aacggccaaa
     7501 ttgcatagag attctccttt ttggtctttt tactatcata cctaaacttt tgtctgatag
     7561 ctagtttttc attattcaag tataattaaa ataaaaaagg gggaagtaaa tgcgtatagg
     7621 aatgcgaaca gtcaaaacag tgatcagtgt cttcttttcg atggtcacag cgggtagctt
     7681 atcgctgttg tattggccag ctgcagggat cattgcatta ttaagtgtag gaaataccaa
     7741 gcgctctaca ttgcgtacgg gcatgaatcg aatcgcagcc tttatattag ctacggcaac
     7801 agcactgttc agtttttttg ctgttgggta tactattttt ggcttcagtc tttttttatt
     7861 cctgttcatc ccgcttgcct ctcgcttcaa attaaccgaa gggatagtag taaattcggt
     7921 tttggtcact cattatttag tggaggaaaa catgtcttgg cagctgatcg gcaatgaagc
     7981 gggattaatg gcgattggat tagtatttgc tttgcttgca aatgtttaca tgccagatcg
     8041 tacgaagcag ctgaaggaaa accaaataca gatcgaaaaa gaatttcgaa atattttgag
     8101 acaaatggca gaatttcttt tatcagaaaa taaagaagat gtgcagatca aatgcgaaca
     8161 tttactgaca ttcattcgcg aaagccaaga agatgccaga gaacatcaag aaaactactg
     8221 gctcagacag ccgctatact acgaaactta cttctctatg agaagagcac aggccaatgt
     8281 gattaaagac atgctggaaa atctagagag aatccagcaa ccagcttatt atggaaaaca
     8341 tatctatggt ttattgattt atactgcaga aacattttca gagtcaaatg atggcagaca
     8401 aattttaaca cgtattgaag aagtttatgt attgtatcgg caaatgcctc tgccgactag
     8461 tcgccctgaa ttcgaggatc gagcagaatt gttccagttt ttgcagtctt ttaaaagttt
     8521 tattgaaatc aaggcagaat tctcccaaca aaaaatccaa aaatagaggt tgtgaattca
     8581 gctaatattc aggtcaaaaa gctgatttca caacctcttc tttaaacggt gttctcctgt
     8641 caactcctcg gtcacaagtc acaatattca acaaacatga gaagctgttt tttgaaattg
     8701 ctccttgtga ctcggagctg aacggtaggc tcacaacctc tttcttaaac ggtgttcaaa
     8761 agctttcctc tgcggtaata agtccagcca aacgaaaaat aggaggagct atttttgttt
     8821 ggctgttctt attacttgat gcagaacagc tttttcacaa ccttttaaaa ttacatattt
     8881 ttccatagaa cttgcatttg atccaagatt tcgatgacat cttttgtcaa gaagagagag
     8941 cgattgggca agtcaccttc aatcatgtgt accatattct ttatttcgta attcattgct
     9001 tcagatgtat tcccgctttc gatgaattcc tttgtaccat ctgtaaataa gatctccgca
     9061 cgatcagcac gcgggtaatc agcaatcgtc acataaccgt tctcaaaggc aacgataccg
     9121 acttttggca tttttgcctg aaaagtcaag ctgacagcag caagttcatt ttctttgtta
     9181 cgcaagatag tgacggattg ttcgtccacg cctgtttcaa aaggaaccat tgtagaggca
     9241 actacttcag gttgagagct caaaaagaaa cgagcgaaag atacagcata cgtaccgata
     9301 tccaacagag ctcctccagc taattctgga ttgaagaacc ggtttttagg gtctggttct
     9361 ttgtaactgc cgaaaggtgc ttggatcatt tttaaagccc ccagttttcc tgtatccatt
     9421 attgagcgta actgctgata aagtggcatg ttgaagattg tcatcgcttc tgctaagatc
     9481 aaattctttt cttctgctaa gcgcatagcg tcagccagtt cttttttatt catcgtgatg
     9541 gctttttcac atagaacgtg tttgcctgct tccaatgctg ccaagatatg gtcgatatgc
     9601 tgtctatttg gtacagcaat gtacacgatg tcgatttcag catcgtctaa catttcttga
     9661 tagcttccgt aagcttttgg aatattgtaa cggtgagcga agttttccgc tttttctgaa
     9721 gttctggaag caactgctgt tagttcgctc gtctcttgat tgaagctttc ggcaaactca
     9781 tgtgcgatgg taccaagacc aacgattccc cattgatatt ttttcatctg aaaacgctcc
     9841 ttcatagttg gattgagttt cttgtctgtc tattattata acaaaaatca cgaagaataa
     9901 agaggagagg acttgaaaca ttaaaaaaaa tgaaagcagc gatttaagag gtgctttttg
     9961 atcaaaactt aagtagaata gttaaaagga ggggataaac gtgcatcaag tatatattga
    10021 tcttattatg agtgtattat tagaacagtt tgaaactgaa aaaaattttt tagatgaata
    10081 cttgctgatc agtccagaac aatggcaaaa atggaaagat ggcgagaaaa atctgccatc
    10141 tgaagcgatg cagaaagtaa aaaatctttt cactgattat gaatggatgc taacgcaaaa
    10201 gattttgcgt caaacgatta tttttcccga aaaacgaaac gttgccgttg cagagtacaa
    10261 gcaaatcaaa acacgcatcg cgcaaaaatg gcttagcagc aaagcagcaa aagtcgagct
    10321 gatccctttg aaaaataatc agcaggctgg tagatacctt gacttgaaag taagtattca
    10381 atatgatgaa tggggctatg atgatatttt gaattttcgt ctgccagcag atgttcaaaa
    10441 acagatcgaa ggagaaaaaa tcgaacttct cgattgggtg aatgaaaatc tggaagaaac
    10501 ttacgttcat gaggcgaatg gggaacgtga agaacaacga aaggattttt caagtgaata
    10561 aatttttagg atttggagca gttgtgatta tgatcgcagc aatgggctat gttattttta
    10621 gcgatgatga ttcgcatgtt caagccggca atcaagcagt cgaacgaaca agcaccaaaa
    10681 caactgctag taaagaaaag aacagtcaaa caaccagcag caaggtaaag gacaaagaaa
    10741 caaaagatcg tacgtccaaa caattgccag atgttaaaac agacgattgg aagctagttt
    10801 tggttggtcc agatcacaaa ttagaaaaag aaattgatga gtctactcaa ttggcatcat
    10861 taagtaacgg atacttgatc gacaaacgca ttgctccaga atatgaagca tttgccaagg
    10921 ctgctgaagc agctggattt ccattagtga tggtctcagc ttaccggtct gtatcatccc
    10981 aacaacaagt tttcgaacaa aatgtccaag atgtgatgag tcgtcaaggt gtatcagaag
    11041 aggaggcgac aaagattact aaagaaacga ttacggagcc aggatacagc gaacaccaca
    11101 caggtctggc agtcgatgtc gtcgatcaaa actggtacaa cagttatcct tctactgtat
    11161 tggatgcaag ttacggagat cagcctggag ctaaatggat cgcagataat gctgcgcagt
    11221 atggatttat cgttcgttat ccaaaaggac acgaagatat aactaagatc acatatgaac
    11281 cttggcactt acgttatgtc ggtaaagaaa acgctgagta tatcaccaaa caccaattga
    11341 cgctagaaga atatctgaat gaattaaatg aaaagtagga attgatcatg gcgaaaaaaa
    11401 agtggaaaaa gcaaaagcgc aagaatatcc gttggccagc agtggttgcc ggtgtagcag
    11461 tgatactctt agcctttgtt ttttccttga agaatctgtc ttctcctttt actgataatc
    11521 agcaagaaaa tgaagcgcaa tcccatcagc aatttattga acggcttgtt ccgcatgccc
    11581 aagaactaca agatggttat ggagtgcttc ctagtatcat tttaggacaa gctatattag
    11641 aatcaaattg gggaaaaagt accttggcta gcaaatacaa taatcttttc gggatcaagg
    11701 cttatgggga tcagaagaaa gtaagcctgg aaacaaagga atttgttaac gaagaatgga
    11761 taactatcca aggagacttc aaagtgtatg attcatggga acagtccatg gatgaccata
    11821 cacaactgtt cgtccaagga gtagattgga atcctgcgct gtatgaaaaa gtgattacgg
    11881 ccaccaacta tcaggaagcc gcacaagcat tgcaagatgc aggttatgcg acagaccccg
    11941 gttatgcgca aaaaatcatc caggtcatcg agacgtatca attgaatcag tatgaccgga
    12001 gatagcaaag gagaagtggc tgatggcaga acaatttacg ccagaaatca tgacggaatt
    12061 gagaaaagat atcgtaagag taccggaaat cattttagaa gcaagcggga tcaagatatt
    12121 tggaaaattg atccgttcga tcatctttac tacggatatt gcgatcatac gtaatacaaa
    12181 tgcgaatgca gtaattgctg tgtatccttt tacacctcat ccagcgatta ctaaaagcat
    12241 tattgaggca gctgacattc cagttttctc cggtgtaggt ggtggtttga cacaaggtgc
    12301 tcgttcttcc tacatgagca tgttcgcaga agcgcaaggt tcgttagggg tcgttttgaa
    12361 cggtcctacg cctttagaga cagtaaaaga agtttgtcga gtagttgata ttccagttat
    12421 cagtacggta acgtcgaaat atacagaaat agatgaaaag ttagcagctg gtatttcgat
    12481 tatcaatatc agtgcaggta aagaaactgc agaagcagta cgccattttc gtaaaaaata
    12541 tcctgaactt cctatcattg cgacaggtgg tccaacggaa gaaagtattg ctgagaccat
    12601 tcaagcagga gcaaatgcca tcacatatac gccaccaagt agcggagagc tttttagtcg
    12661 taaaatggaa aaatatcggg aaactgagaa aaagaatcat gaataacagg tgagaatcaa
    12721 aagacgagga agagcagata gtggctcttc ctcgtctttt gtactccagt actatttgtt
    12781 cagaattcaa aaaaaagaga aatttttttg tttttttacc tgtaatgtac ataagaagag
    12841 ttaaaacctt ttgttattat tagcattcta acaaaaaaat gtcttgattt attgaagcgc
    12901 tttctataga atgaaaaacg aataaaaact aaaaaaggca cccgacaatt tagtagcagc
    12961 taaattgtcg gggcttgaaa agtcagcggg aactgaccaa tcaagttgcc atagtcaatg
    13021 cttgtgcatc gacgtgtata ttgtacctaa tttcatacag aattactagt tatttttctg
    13081 ttttttaggg aagagcgcac acgaagaagc atgcttattt agctatgtca aggatattgg
    13141 gcagttgtct gatatccttt tttctttgcg taattatcct cccatcattg aagcaagaag
    13201 tgtttttagc gatgaaagaa gaatcagcac tatctaattt ttcttgaaag tattgagttg
    13261 ttcatcctag gcagaacgat aaggcaagtc ttggttttta ttccccttga aaaagatcgt
    13321 ttaataaata tgttctgtat aaaaatacag tatattgaaa aataacaatg gaagtagagg
    13381 tgcagagaat gaagaaaaga atgagaatca tcttagcttt aagtgttatg ttattcttct
    13441 tgacggcttg tgggcaagaa gatgacaaaa aaatcacgct gaacatggct tggtggggtt
    13501 cgcaagtccg acacgatgca acagtaaaag taatcgagct ttatgaagaa aaaaatcccc
    13561 atgtgacgat cgattatgaa ttttatgatt atgaaggata ttacacaaaa ttaggtacac
    13621 tagcagcatc agatgatatc tgggacattt tccaactgaa caatcaattt cctcaataca
    13681 tcaatcagat tgaaccatta gatgaatatg tggagcaagg gataattgat atttctaatg
    13741 cagatgaatc ctatttagca acgacgactt acgacgggca attagttgga ctgtctaacg
    13801 gggtgaattc gtttgcgatt gcttacaata aaaatatatt tgaacaatta aatatccctg
    13861 aaccagcttc aaattggaca tgggatgaat ttgaacaaat cagctatcaa atcactgaag
    13921 aattaggaat ctatgctatt tcaagattcg aagattttat agctggctgt attattcaaa
    13981 taccacaagt tgagaaagga ttgaattttt ataatcggga agatctatct aacagcttgg
    14041 gatttgaaaa tccagactat ctaacagatt ttttccagct gcgtaaaaat ctggtagaca
    14101 aaggggccca tccagaacca ggtggtggag ctgcaagtac gacggatctg tcttttcagg
    14161 cggttaggga tagagaagcg gcaatggtgt ttctatcaag taaccagttg acagaaatat
    14221 tgtcaggggc tcctgaagat atggagattc agttgattaa tccacctcgc agaaaagcag
    14281 atggagaatc tggtatccct ttacgttctt cgcaaatgct gagtatggca aaaagctcag
    14341 cccataaaga agaagctgct aagtttattg atttctttca aaacagtgaa gaagccaatg
    14401 aaattttaaa aggagaacgt ggtgtaccaa tcatgtcgaa tattcgagaa cagctcgctc
    14461 gtgcagatga tgcaactttg caagcgacat ttgattattt agaaatgatt tccgatatgg
    14521 acaacggcga agtgaatgtc tttgatagtg ctttgaatcc ggaaattcaa gaccaataca
    14581 atcttttagt tgaaaaagtc atttatgaag aagtaacagc agaaggaagg catgaaaaaa
    14641 gaaaaactaa aacggttctt ggatcaagac aatgtggtcg gttacgtatt tgcttctcca
    14701 ttcatcctag gatttctatt gataacagtg ataccgatga tcttgtcact ttattattcg
    14761 ttcaccgatt atcgtttggg aaatccagtc tcatggatcg gcttggataa ttatataaag
    14821 atgctgagtg atgcccgttt tttgaactcc atccgagtca cttttctcta tgtatttgca
    14881 tctgtacctg tgaaactgat ctttggtcta ttgattgctt atcttttaac gaggaatatc
    14941 aaaggaacga ctttctttcg ttctttgtat tatgtgccta gtttgatcgg tggcagtatt
    15001 tctatcgctc tagtttggcg ggagttgttt tctaaaaacg ggttagtgaa tattttcctg
    15061 gaacgattag gcgcacctgc gctttcctgg tttagcgatc aaaaactggt catgatccct
    15121 cttgttttga tgtctgcttg gcaattcggt tcggcaatga tcatctttgc tgcaggtatc
    15181 aaagagatcc caggctcgta ttatgaagcg gcagagatcg atggagcgaa caaatggcag
    15241 acgttttaca agatcacgtt acctcttctt tcgccgatca ttttgtataa cttggttatg
    15301 cagacaatcg cggcgtttat gacttttacg caggcattta tcattaccca aggtggtccg
    15361 aatgatgcca cgaactttta tgctctttat gtgtataacc aagctttttc ttttaattct
    15421 atggggtatg ctagtgctat ttcgtggatc atgctgttga ttatgggagc tgtcaccttt
    15481 ctgattttca aatcatcaaa aaaatgggtc gttgtgtgaa tgagaggagg aagaaatgaa
    15541 taatacagta acgaaatggt ttcaatacgg ttttattttg ctattaggtc tagtcatgat
    15601 ttatccggtc ttatggatga tcgcaggttc ttttaaaacg gatcaagaga ttatgagtgg
    15661 ttcgctgaat ttgattccag agacttttca atgggaaaat tacgcaaaag gatgggcggg
    15721 gtttgctgga atttcatttt ttactttctt taggaattcg tttgtcatct ctgcagtttc
    15781 gacgataggg acagttctga gctcgacttg tattgcttac gcattatcga gaatcgattt
    15841 caaaggaaaa agattctggt tcgtcgtcat gttagtaacg atgatgattc ctgcacaagt
    15901 gattttgatt ccgcagttta ttatttacaa tcggctgaat ttggttggga cgtatgtgcc
    15961 gctgatttta ccacattttt ttggacaagc atttttcatt taccagatca tgcaattcat
    16021 ggtcagcatc cccaaagagt tagatgaatc agcgattatt gacggatgca gcaagtatag
    16081 tgtctttacc aaaattattt ttcctttgct gaaaccttcg atcatcacga cgatcatcat
    16141 ccagttttat tggaaatggg acgacttcat gggaccattg atttatttaa acaagccgag
    16201 atcttacacg gtatcgattg ccatcaaatt atttgcagac gctggatcta gtacgactta
    16261 cgcgagtatg tttgcgatgt ctacgttgtc gctgctaccg gtttttctga ttttcttatt
    16321 cttcaataaa tatcttgtcc aggggatcag tacaagcggt ctgaagggct gaatatgaaa
    16381 aagccaatag aagagatgtt tctttacaag ctcaactact atgtctgtcg gattttttta
    16441 accagtctta tcagtaatgc tcttttcgct ttagttctag gatgtttgtt cactttagga
    16501 ttagtgctgt ctgctcgtgt tgtctatctg atttattttt tgatgatgta tccttcttat
    16561 tatctatctg tccattactt ggcagaaaaa aaggtatgtt ggaggaaagg gaaaatcaaa
    16621 cggatgtgtt tcatcattac gttgtttcag ctgtttgtca ttatttttgc agttgattgg
    16681 tatttattag tgggaaagat tggagcttgg tatctgattc cttttctatt gtgtctgttg
    16741 ctttttgtat cccatagttt ttttagtacg ctaattgata tctgcaaaga agataaagca
    16801 gatagtcttg tttatttgat caaaaggaat atctggctgt tgtcaaaaaa cgtcaagggg
    16861 tttctgtggt atttatgcct tttgtatgga ctgttctggt gtatacagcc tgatttttta
    16921 ctcactgcat tactgtatcc agggctgcca tttgtcacag tattttgctt aaaagaaaag
    16981 gagaatgact ttaaatgaag aaaaaatatg tacaagtcgg acttggtggg agagcgcgtt
    17041 ttttttatga ggcaattgcc tctacgtatc atcaaacttg tgaactagtc ggtttttgtg
    17101 atgtatctca aacaaggatg gattatgcaa atcggatact tgtgaacaag tatggacaga
    17161 aaaaagtaaa gacatgtatg ccatcacaat ttgagcaact actggatgac gtacggccgg
    17221 ctgaagtcat tattacgtca gtagatcgaa cgcatcatga ttatatcatt cgtgcaatga
    17281 aaaaaggata tgatgttatt tgcgaaaaac ctctgactat cgatgaggaa aaagctcaag
    17341 aaataataac ggtccaaaaa cagacgggaa gaagcctgcg agtcactttt aattatcgtt
    17401 atgcaccgta tcacacgaag ataaaggaac tgatagaaag tgatgtgatt ggtgatgtgt
    17461 tttctatcca cttcgaatgg ctgttaaata cgcaacacgg ggcagattat ttcagaagat
    17521 ggcatcgaaa taagcacaat agcggcgggc ttcttgttca taaatctacc catcattttg
    17581 atctagtgaa tttttggttg aacagccgtc cccaaaaggt atttgcattc ggtggattaa
    17641 acttttatgg gatgcataat gcggagaaac gcggagtaga aatattttac agtcgatgct
    17701 atggaagcga actagctaaa gctgatccat ttgctataga cctgtcagcc aatgaggaat
    17761 tgcgagaaat gtatttagaa gcagaaaaag acagtggata tattcgtgac cgcagtgtat
    17821 tttccgatga tatctctatt gaagatacac taggcgtaac ggtcaaatat gaaactggtg
    17881 cgattttgac gtattcgcta aacgcgtata tgccgtggga aggattcaat gtttctttca
    17941 atggcagcaa aggacggatc gaaatgaaag tgatcgaaaa gtcttacatc aatgcaggtg
    18001 gagataaaaa ggatgaagga gcagccgttc ttcagtccat ccatgtctat ccgatgttcg
    18061 gtgagccata tgaggtaatc gtcgaacaag aagcgggcgg acatggcgga ggagatccgc
    18121 gtttattaga tgatctattt ggtgaagaaa aagaagaaga tccgtggaat cgggcagcaa
    18181 ctcacgtaga tggtattttg tcgattttga caggaatcgc agcaaatcat tctattgctt
    18241 caggaaaagc agtagtgatt gacgagcttg tcagcttaaa agaataagaa tagatttacg
    18301 gaaattactt cccgtagttt ataaaatctt gccttattgc cgataggctg tcttttgaac
    18361 actatttatt cggaaaaagg ggctatgaca tgctttttgt catgccttct ttttatgtgg
    18421 gctgcttttt tagataggag aaaataaaaa taactattta aacccgaaca aaaaggaata
    18481 atgcattaaa aatcattaga ttttacaaaa aaataagtaa accacgttgt cagtttcaga
    18541 aagtagtgtt agactgtttt caatatttga aacaacattt tttattcaaa tatggcaaac
    18601 tgccttcacg tgcaaaacag ttgcaagaaa atgaatttac actattatta gttttacata
    18661 cctacttata tatcgtcata atatggatga cagtttctag ccagtaccgt aaatgctgga
    18721 ctataagtaa aagattggct atttcaccct tcttttctta tgataaagtg gagggtgttt
    18781 ttctttaaca gaaacatagt aggtttttgc acaaaagcgg ttatttcggt attaataaat
    18841 gataaaggag caaataaagt gaaagaatta gttgaacgta ttcaaaaaga cggacgtata
    18901 ttaggcgagg gcgtattaaa agtcgacagc ttcgtcactc accaagtaga ccctgtattg
    18961 atggaacaaa tgggaaaacg gtttgcagaa gtatttaaag aacaaggaat cacgaaagtt
    19021 gtgaccattg aagcttctgg gatcgcacca gctttatttg ctgcaagaga attagatgtt
    19081 ccaatgatct tcgctagaaa agcaaaaagt ttgacgatgg atgaagaatt attgacagct
    19141 tctgtttatt cttttacgaa acaagtgaca agtacgattt ctatttctcg taaattttta
    19201 tcttcagaag acaaagtatt gattatcgac gatttcttgg caaacggaca agcggcaaaa
    19261 ggattgatcg aattatgcca gcaagctgga gcgcaagtag aaggaatcgg gatcgtcatc
    19321 gaaaagtctt tccaagatgg acgtcagtta cttgaagaaa tgggattgag agtcgtttct
    19381 cttgcgcgta ttgcttcctt gacgaatgga caagttgagt ttcttgagga ggacgcataa
    19441 tcgtggaaaa agaaacacaa aatggaaaag ctgcggtttt agggctgcag catctgctag
    19501 cgatgtatgc aggagcagta gcggtgcctc ttttgatcgg tacaggacta ggctttaatc
    19561 aagaacagat gacttatttg atttcaattg atatctttat gtgtggatta gcgacattat
    19621 tgcaattgac agttaaccga tttttcggaa ttggcttgcc tgtcgtttta ggatgtgcga
    19681 tccaagcagt agctccactg atcttgatcg gaaccaatga aggagtagga gctatctacg
    19741 gatctattat cgcttctggg atctttgttg tgttagtttc tggtgtcttt tctaaaatta
    19801 aaaaactatt ccctcctata gtaacaggaa cggttatcac cgttattggt ttgactttga
    19861 ttcctgtagc aatcgagaaa atgggtggag gtaatgcaac agcagctgat tttggtgaca
    19921 aaacaaatct gctacttgca tttgtgacga ttctcctaat tgtaggcgtg caaatgctgg
    19981 caaaagggtt cattcgttca atcgcggtat tgattggttt agtcggagga agcatcttag
    20041 ctgctttcat gggaatgatt gatctgagcg cgattggaga tgcgccgttg ttccatgttc
    20101 cacaaccctt ttattttggt aaaccaactt tcgacgtatg gtcgatttta ctaatgatca
    20161 tcatttccat ggtcagcatg gtagaatcaa caggtgtata ttttgcactt ggtgacatta
    20221 ctgggaaaaa tgttggagaa gaagaattga aaaagggata tcgtgcagaa ggacttgccg
    20281 ttatcttagg cggtattttc aatacattcc cttatactgg cttttctcaa aatgtcggat
    20341 tggttcaact ttcgggaatc aaaacaagaa aacccatcta tttctctgca ttcttcttga
    20401 ttgttttagg attattccct aaaatcggtg cgttagcaca aatcatccct gagccggtac
    20461 ttggtggagg aatgttagtc atgttcggga tggtggcagt ccaaggaatc cgtatgctgt
    20521 tggaagttga tttcagcaat gataaaaatc tattgattgt tgctgtttct gttggatttg
    20581 ggctgggttt caatgttacg ccgacattat tcagtcaaat gccgcaaaca ttgcaaatgt
    20641 tcactggaaa tggcatcgta atgagtagca ttacggctat catattgaat ttgatcttta
    20701 atggattgaa aaaagatgaa gaaatattgg aagaagcctt caagaagtaa tagagcaaaa
    20761 gaaaggttgt gaaatcagcg ttttgacctg attgttagag aaacaaacgg aaaaatagtt
    20821 cctcgtattt tgccgtttgt ttgagctaat accgagggtc agctgattga acaccgttta
    20881 tccataaaaa ggaatgtgac ctaacttttg tcacattcct ttttttcatc tttaataaac
    20941 acgaacaata aaatgttttt ctttgttaac attcgaaatt tctttgacat caaaaaaagt
    21001 gattgttaaa atatgaatgt agaaaaactg ggaggaaaaa caatgacagc aaaaatctct
    21061 gtgattatgg ggagtacttc agattgggaa acaatgaaag aagcatgtca aatattggat
    21121 gaattcggtg tgtcttatga aaaaaaggtc gtttctgctc atcgaacccc agatttgatg
    21181 tttgaatatg cacaaaacgc tcgaaaaaaa ggaatcaaaa tcattattgc aggagcaggg
    21241 ggagccgcgc atctgccggg tatggtcgca tcaaaaacaa ctttgccggt tatcggtgta
    21301 ccggtccaat cacgaacatt aaatggtttg gattcgcttc tttcgattgt ccaaatgcct
    21361 ggaggagtac ctgtagctac tacagcaatc ggaaaagcgg gagcaattaa tgctggacta
    21421 cttgcgatac agatgctttc catgtatgat ttagaattgg aggctaaatt ggctgaaaga
    21481 cgttcaatgt tggctaaaac tgttatagaa agtagtgatc aacttggtta aacctttgtt
    21541 gcctggttca acgatcggga tcgtcggtgg cggtcaatta ggtcgtatga tggcgattag
    21601 tgcaaaagaa atgggcttta aagtaggtgt gctggatcct gtgagcgatt gtccagccgc
    21661 acaagttgca gattggcaca ttgaaagtgt ctatgatgac acctttgcgc tgaaagagct
    21721 tgccagaaga acagatgtca ttacctatga attcgaaaac gtcagtgtag aggcgttaaa
    21781 tgcgatttta cccatgtcat ttattcctca agggacagac ttgcttgcga ttacgcaaga
    21841 ccgcctgtta gaaaaatctt ttttggaaac gaataacatc gtgattgctc cttatgcaac
    21901 gatcgtcagt ccgacggata tccaagatgc gattgacggg atcggctatc cttgtgtttt
    21961 gaaaaccact cgaggcggct atgatggcaa ggggcagtat gttttgaaaa gtcgggcgga
    22021 tctagctcct gccatggacc ttttaagaga aggaacctgt gttttagaag catggatccc
    22081 gtttgaaaaa gagatttcga tcatggtggc aggaaatggt caaggagatt tcacgacttt
    22141 tccggtcgtg gaaaacatcc accacaacaa tattcttcat gaaacgattg ctccagccgc
    22201 gattgatcag gatgtgatcg aggaggctga gcgtattgcc cgtgtgattg cagaagcagt
    22261 gagcctttca ggtgttttag gggtagaaat gtttttaaca aaaacaggcg ggctatatgt
    22321 gaatgaatta gctcctcgtc ctcacaattc tggacattat tctattgaag cttgttctat
    22381 gagtcagttc gatgcccata ttcgaggtat ttgtggttgg ccattaggag aaaaagtacg
    22441 tttgataagc aatgcagtaa tggtcaatgt tttgggagaa caactgctgg aaagctatcg
    22501 cttgattgag aataagccag actggcaatt ccatttctat ggaaaaaaac aagcaaaaaa
    22561 aggtcgcaag atgggacata tcactatctt gacagaagac atccagcaaa cgctggaaga
    22621 aatcaaacaa acgaatattt gggattagag gagaaaaaac atgttagaac gttatacacg
    22681 ccctgaaatg gggaatatct ggacggaaga gaacaaatac cgtgcgtggc tcgaagtcga
    22741 gattttagca gatgaagctt gggcagaact tggtgaaatc ccgcaagaag atgtgaaaaa
    22801 aattcgtgag catgcatcat ttgatatcga tcggatcttg gaaatcgaaa aattaacacg
    22861 ccatgatgtc gttgcgttta cacgagcggt ttctgaaaca ttgggagaag aaagaaaatg
    22921 ggtccattat ggtctaacaa gtacggatgt cgtagatact gcttatggct atctcatcaa
    22981 gcaagcaaat gatattttac gggaagactt gaaacgtttt acggaaatca ttggcgaaaa
    23041 agcaaaagaa cacaaatata cagtgatgat gggtcggaca cacggtgtcc atgctgaacc
    23101 aactactttt ggattgaaac ttgccacatg gtattcagaa atgaaacgga acatcgaacg
    23161 atttgaacat gcggcaaaag gagtagaagc aggaaaaatc tcaggagcag tgggaacgtt
    23221 cgcaaatatc ccgccattta ttgaagagta tgtatgtaaa caattgggga tccgtccaca
    23281 agaaatctct actcaagttc tccctcggga tctacatgct gaatatttct cagcgatggc
    23341 gcttattgct acaagtattg aacgttttgc aacagagata cgcggattgc aaaaatccga
    23401 aacacgtgaa gtagaagaat tttttgcgaa aggtcaaaaa ggttcttctg ctatgccgca
    23461 caaaaggaat ccgatcggtt cagaaaatat ggctggtcta gccagagtgg tgcgcggaca
    23521 catggtcact gcctatgaaa atgtcacatt atggcatgaa agagatattt ctcattcttc
    23581 agcagaacga atcatcttgc ctgataccac gattttattg aattacatgt taaatcgttt
    23641 tggcaatatc gttaaaaatc taacggtctt tcctgaaaac atgaagcgga atatgtctgc
    23701 aacatttggt ttgatttata gccagcgcgt tatgctgaaa ttgatcgata agggaatgac
    23761 aagagaagaa gcttatgatc ttgttcagcc aaaaactgcg tacgcttggg acaaccaagt
    23821 gcagttccgc ccgctgttag aagaagatcc aaccatcaca tcggtattat ctaaagaaga
    23881 aatagacgat gcctttgatt atcatcatca tttgaaaaac gttgacgtga tattcaaacg
    23941 agtaggtttg gaataaagaa gagtgagttg caaaattcaa ttaaaataga atagcaaaaa
    24001 taaccaaaaa agaagcagat ccgttagcgg ttctgcttct tttagtttat tatattttct
    24061 tatcaataat aggaacctga ttttaaaagc ggctacctct ttaccgtgcc tctttacata
    24121 caaaaaatag aggaattaag cgtaaatggc ttggctatta taactaaaat gctggttatg
    24181 gagatgttgc agcaagtttt cgagaaaagc gtattccatc tcttgtccat tgaataagat
    24241 ctggatacga taatgatttt tgaaacgctt ctgttcatga atctgactga ttgaaagttc
    24301 gcctttgtgt tctgtctgat aaagaaaagt ccttttacgg cattcagcac agcgcttggt
    24361 tgctcggcgg atttttcgga atgtataaaa actagtatct ttattgcata aataaacgtc
    24421 tttgatcgac agcaaatcat tatatagaca ggaacgttct tcagctgtta attctgataa
    24481 aaaaagctga ccttgtttta ttgcatcttc catcggcaag tgcctcctcg aacccattat
    24541 ccaaattttt agttaattct aatataccat cattaagtat aaatgtaaac gcttgttgat
    24601 ggtataagat tttcgtttct gataaaacac gaacattaaa agaatctaat gccttaaatg
    24661 tacatatttt tgttgattta taaaaagatg attgctaaaa tttaaagtga aaaaacgaac
    24721 aaaaaacgat gtatttgctt ttaatgtaaa taaaaaggag agacgtacat ggagaagaaa
    24781 gaacttttgt atgaaggaaa agccaaaaaa atatatctta cttctgatga gaacttgatt
    24841 tggatagaat atttagatca agcaactgcg ttgaatggcg cgaggaaaga ccagataagt
    24901 ggaaaaggag aattgaataa tcaaatcaca agtgcaattt tccgttatct gactcaaaga
    24961 ggaataaaaa atcattttat tgaacaattg tctaaaaccg aacaattagc aaaaaaagta
    25021 caaattatcc ctctggaagt ggttatccga aatattgcag caggaagttt ctcaaaacgt
    25081 ctaggtgttc ccgaaggtaa agaattcatc aaaccaatca ttgaattcta ctataaggat
    25141 gaccagttgg atgatccatt tatcaatgaa gagcatatct atttcttaca acttgcaaaa
    25201 ccggaagaaa tcagtgagct gaaagcccaa gcgttggcta ttaatgaaat tttgaccgaa
    25261 ttatttgcta aggtcggttt ggttttagta gatttcaagc tagaatttgg ccggtcgaat
    25321 ggacaaattt tgcttgcgga tgaagtaaca ccggatactt gccgattatg ggatgaacgt
    25381 acgaatgaac atatggacaa agacgtttac cgcagagatc taggtgattt gatcccagtc
    25441 tatcaagaag tatataaccg tctatcggaa ctaactgaag aaaaggagcg agtatgatgt
    25501 atttcgtaaa agtatatgta acctataaag attcagtgtt agatcctcaa ggggaggcag
    25561 taaaaaaagc agtccatcgt atgggatatg aaacaatcga agatatccga ataggaaaat
    25621 actttgagat acaacttgct gatgcagaag aaccaatcga tacagtgatt gaaacgattt
    25681 gtgacaagct gcttgcaaat gtaaatatgg aaacataccg atatgaaatc aaaaaaatgg
    25741 aggaagtata acatgaaatt tgcggtcatc gtttttccag gttcaaattg tgacgcagat
    25801 atgttatggg cagttcgcga gatcatgggc gctgatgccg aatttgtacg gcatgatgag
    25861 tcctctttag aaggatttga tggggtttta ctgccgggag gattctccta tggtgactat
    25921 ttgagatgcg gtgcgattgc tcggttttct acaatcatgg aagaagtgat tcgttttgct
    25981 gaagaaggga aaccagtatt cggtacatgc aatggattcc agatattgac agaagcagga
    26041 cttttgccag gtgtactgag acgaaatgag tctttgcatt ttatttgcaa aactgtacca
    26101 ttgaaagtca accccaagac ggcctttact tcagagtatg aagccgacga aataatctat
    26161 cttccagttg ctcatgggga aggaaattat ttctgtgacg aagaaacact tcaagaacta
    26221 aaaaaaaata atcagattgc ctttacttat aatgacaaca tcaacggcag cgtggaaaac
    26281 attgcaggga ttacgaacaa agcagggaat gtgttaggaa tgatgcctca tccagaacgt
    26341 gcagtagaag acttgctagg atcagaagat gggaaacggt tctttgcttc cattttaaaa
    26401 aacttcggaa aggtgaccgt atagatgaaa acattgaaag aaccgacagc tgaagagatc
    26461 aagaaaaaaa gaatctatgc cgaatgggga ttgactgatg aagaatatcg aatgatcgaa
    26521 gaagatatct taggaagaat gcctaattat acggaaactg gactgttttc tgttatgtgg
    26581 agcgaacatt gttcctataa aaattctaaa ccggttctac gcaaatttcc tactagtgga
    26641 cctcatgtgt tgcaaggccc tggagaaggc gcaggcatcg tcgacatcgg tgatggacta
    26701 gcagttgtct tcaaagcaga aagtcacaat catccttcag ccgtggagcc ttatgaagga
    26761 gctgcgacag gagttggcgg aatcatcaga gatattttca gcatgggggc tcgtccaatt
    26821 gcgttgctag attcattgag gttcggcgaa ttagataatc caagaacgaa atatttacta
    26881 gaagaagtag ttgcaggtat cggcggatac ggcaattgta tcggcatacc gactgtaggc
    26941 ggcgaaattg catttgatcc ttgttatgaa ggaaacccat tagtcaatgc aatgtgcgta
    27001 ggtttgattg aacacaaaca tatccagaaa gggcaagccg ctggcgtcgg taattcgatc
    27061 atgtatgtag gtgcaaaaac aggtcgcgac ggtatccacg gagcaacttt tgcctcagaa
    27121 gaatttaatc aagaagaaga acagcaacgt tctgctgtac aagtaggcga tccttttatg
    27181 gaaaagctgc tgcttgaagc gtgtttggaa ttaatcttgc atcattcgga ttctctaata
    27241 ggtatacagg atatgggagc agcaggactt gtttcatcta gtgcggaaat ggcttcaaaa
    27301 gctggctcag gacttatatt gacattagat gaagtcccac aaagagaaac tgggatgaca
    27361 ccttatgaaa tgatgttatc ggaatcacaa gaacggatgt tgatttgtgt taaatcagga
    27421 gaggaagaaa aaatccaaga actatttcag aagtatgatc tagatgcagt cactatcggt
    27481 aaagtaacag acgacgggca atatcggcta tatcatcaag gagaagaagt agcaaatctg
    27541 ccagtcgatg cattagctga agatgcacca gtttatcata aagaaatgaa agaaccagct
    27601 cgaatcgtag aatttcagca gttagctcct tatcagccag ttatcgaaga acctggacaa
    27661 gtattgcttg atctgctgca gcaaccgacg attgcttcaa aacgttcaat ctacgaaact
    27721 tatgattcac aagtacaaac caatacagtt gtccgtccag gaagcgatgc tgctgtgatg
    27781 agggttagag ggacgaataa agcgttagct atgaccacag actgcaacgc gcgctatctg
    27841 tatttggatc caaaaatcgg aggacaaata gcagtggcag aagctgctag aaatattatt
    27901 gctagcggtg gtaagccctt ggcgatcacc gactgcctga actatggttc tcctgataaa
    27961 ccagaagttt tttgggaatt atcgacttca gctgacggaa ttgctgctgc gtgtgaaacg
    28021 ttaggaactc ctgttatttc aggaaatgtc tctttatata atgaaacgga tggtcaagca
    28081 atctatccaa caccaatgat tggtatggtc ggattgattg aggatcataa acatatcact
    28141 actcaggaat tcaaaaaatc aggggatcta atctatatat taggtaagac gtttgcagat
    28201 tttgatggca gtgaattaca aaagatgcag cttggaagaa tcgaaggtgt gatccgaaac
    28261 tttgatctat ccattgaaaa gagaaaccaa gagttagtat tgacagccat ccaaaacgga
    28321 ttgattgaaa gtgctcatga ctgttcagaa ggtggcttgg caattgcact ggcagaatca
    28381 gctttcaaac atcagctagg gatatctgta cagtttgaat tgtcctctgc tcaattattt
    28441 gcagaaactc aatcaagatt tgtgttgaca gtcgcaccgg aaaataagac acgattcgaa
    28501 gaaatgatgg gagatgctgc tgttttagca ggtaaggtga cggatgaagc gataatcgaa
    28561 atttcagcaa ctgacggtca gatcaaaata gaaactgcag tagcaagaaa gtgctgggag
    28621 gatgcaatcg tatgtcttat gaagtgaaga gtttaaatga agaatgcggg atcttcggtg
    28681 tttggggtca tccagacgcc gctagtgtgg catacttcgg gctccatagt cttcagcatc
    28741 gaggacaaga aggtgcaggt atcgtgtcca atgatcgagg aaagcttaag gggcatcgag
    28801 atttggggct gctttctgag gtattcaaag atcaaagaaa attgcagcag ttaacaggag
    28861 aagctgcaat cggacatgta cgttatgcaa cagcaggcaa tggaagtgtg gacaatattc
    28921 agccttttct attcaaattt catgatcagt cgatcggctt agcgcataat ggtaacttga
    28981 tcaatgctcg ttcccttaga aaagagttag aggaaaatgg ggcgatcttc cattctaact
    29041 ccgatacgga gatattgatg catttgatcc gacgaagtga agaaacagat tttttaaata
    29101 aattaaaaag tgctttgaat gaagtcaaag gtggctttgc ctatttatta atgacagaaa
    29161 cggcaatgat tgccgctttg gacccaaatg gttttcgtcc attagcaatc ggacaaatga
    29221 aaaacggagc atacgtgatt gcctcggaaa cttgtgcatt agaagttatt ggggcacgtt
    29281 ttataagaga tgtccagcct ggtgagatag tgatcatcaa tgacgaagga atccagattg
    29341 attgctttac gaaggacacg cagctttcta tttgttcgat ggaatatatc tattttgcca
    29401 gaccagattc aaacatcgca gggatcaatg tgcatactgc acggaaaaat atgggacgga
    29461 atttagcgaa agaatcgcca gtaaaagcag atatggtcgt tggcgtacct aattcttctt
    29521 tgtcagccgc aagcggttat gcggaagcta gtggaatccc ttatgaaatc ggattagtaa
    29581 aaaatcaata cattgcccga acatttatcc agccaacgcc tgagctaaga gagcaaggtg
    29641 ttcgaatgaa attatctgct gtacgaggag tagtggaagg taaaaaggtg attatggtcg
    29701 atgattcaat tgttcggggt acgacaagca gacggatcgt ccatcttttg aaagaagcag
    29761 gggcaaaaga agtccatgtg aggattgctt ctcctccttt gaaatatcca tgtttttacg
    29821 ggattgatat tcaaacgcgt aaagaactca ttgcggcaaa tcacacgatt gatgaaatca
    29881 aagaatgtat cggtgcagat tctttaggtt ttttaagtga agagggtctg atcgaatcga
    29941 tcggtctgga aaaagatgct ccgtattcag gattgtgtat ggcttatttc aacggagatt
    30001 atccgacgcc gttatatgac tatgaagaaa aatatcagga atcactaaaa gaaaaagtat
    30061 cgttctttta aaagcagacc aagaagggag tatatgaatt caatggcaaa tgcatacgca
    30121 aaagctggcg tagatgtcga agctggatat gaagtagtag aacggatcaa gaagcatgtc
    30181 aaaaaaacgg aacgtttagg tatgatgggc gctataggag gattcggcgg ttgttttgat
    30241 ttatcggtct ttcaattaaa agagccagtc cttgtctcgg ggaccgatgg cgtggggacg
    30301 aaattagcag tggcaatcga agcagacaaa cacgatacaa tcgggatcga ctgcgtagcg
    30361 atgtgtgtca atgatatctt agcacaaggc gcagagccgt tatatttctt agactactta
    30421 gcaacgggga aaaatgatcc gttacgtttg gaaaatgtag taaaaggtgt agcagatgga
    30481 tgtgttcaag gaaatatggc gctgattggc ggagaaacag ctgaaatgcc tggtatgtac
    30541 tcaggtaatg attatgatct agccggattt gctgtaggga tagctgaaaa atctcaatta
    30601 ttgacaggag aaacgatcaa cccaggagat gtgttgcttg gaatagcatc tagcgggatt
    30661 cattcaaatg ggtactctct tgttcgcaaa attttctttg agcagcagca gttttcttta
    30721 tcatcaaaac tgccagaatt gaaaaaaaca cttggcgaag aattgcttga gccaacgaaa
    30781 atctatgttt cttccttgct tccgctgatc aaagaaaaga aaataaaagg agctgcacat
    30841 atcactggtg gagggtttgt agaaaatatc ccgcgaatgt taccagaatc tatggctgca
    30901 caaatcaaac ttggctcatg gccgattctt ccaatattcg gctgtttaca aaaatatggt
    30961 gaaattcctg agatggagat gtatgaaatc ttcaatatgg gcatcggtat ggtcctggca
    31021 gtcgaagaat cgaaagtaaa agaaatagta cacgaaattg agcaagctgg cgaaaaagtt
    31081 tatgaaatcg ggaaagtgat cgccaaagaa aaacatcgtg tggtgtttat aggagaagaa
    31141 cgatgagaat cgctgttttt gcttctggaa acggaagcaa ctttcaagcg ctagccgatt
    31201 atctatcgaa aaaaggattg gaatcttcga ttgactggtt gttttgtgat cagcctgaag
    31261 catatgtttt gaaacgtgca actgcgttga gtgtaccggc agattgtttt tcacctaaag
    31321 aatttgattc aaaaaaagag tatgaagaag caattttgca taaattgaaa gaaaaaaaga
    31381 tcgatttgat cgtattagct ggatatatgc gaatcattgg accggtattg ctgaaaaatt
    31441 atgataaaag aatcatcaat atccatccgt ctcttttacc tgccttccct ggtttgcatg
    31501 gcatacgtga tgcctttgaa gctggagtaa aagaaacggg tgtcacaatt cattatatcg
    31561 accaaggtgt agatactgga ccaatcatcc ggcaggaaaa agtgagaatt gaacaggagg
    31621 acacgttcga ttctttagaa gaaaaaatcc atagagtaga gcatcgaatc tatccagaag
    31681 tgattagtga aattattgaa aatggaggct atctgaaatg acaagagcat tgatcagtgt
    31741 atcagataaa actgggattg tagcattcgc ccaaggtttg attgaacaag gagtagaaat
    31801 catttcaaca ggaggaacaa agcatgcgtt ggataaagct gggatcaaaa cgctttcgat
    31861 cgaagaagtg acagggtttc ctgaaatgat ggatggccgt gtcaaaacac ttcatcctaa
    31921 aatccatggt ggtcttttag gaagacgcga tctccctagc catatggagg caatggaaac
    31981 ggaaaatatc caaccaatcg actttgtttg tgtcaattta tatcctttca aagaaacgat
    32041 tatgaaacca gatgtcgaaa tagcagaagc tattgaaaac attgatatcg gtggtcctag
    32101 tatgctccgt agtgcagcaa aaaaccatga atttgtcacc tctgttgttg atccatctga
    32161 ctatgaagaa gtactttcag aattacgtca gtatgggcat acttcacttt ctttcagaca
    32221 aaaactagcg gcaaaagtct ttcgtcatac tgcggcatat gatgcactga tcgctcaata
    32281 cttaacggat tgggtggaag aaaaagaacc ggaaaaattg acacttacgt atgaacgaaa
    32341 acaaacgctt cgttacggtg aaaatagcca tcaagccgct gccttttatc agtccgctct
    32401 tctggaaaaa tattcgatcg cagcagcaaa acaactccat gggaaagaac tttcttataa
    32461 taatatccga gatgcagatg ctgcgctacg gatcatgaag gaatatgacg agccaacggt
    32521 agtcgccttg aaacacatga atccttgtgg tattgggaca gcatctacga ttttagaagc
    32581 gtgggaagca gcgtatgaag cagatcctgt ttcaatcttt ggtgggatca tcgttttgaa
    32641 tcgagaagta gatctgccaa cagcagaaca gatgcataag ctgtttctag aaatcatcat
    32701 tgccccttct tatacacaag aagctttgga attactgcaa aaaaagaaaa acatccgatt
    32761 actgacagta gactttgcgc aggaagaaaa tcaaaatcga gaagaaacgg tctcagtctt
    32821 aggtggactt cttgtacaag atcaagatct ggcaatagaa aatgaagaag agtggaaagt
    32881 cgtgacaaaa cgtaagccga ctccagcaga agcaaaagca ttagcgtttg cttggagagc
    32941 tgtcaaacat gtgaaatcta atgcgatcgt actggcaaac gaacgtcaga cagtaggaat
    33001 cggagccggg cagatgaatc gagtcggttc agtaaaaatt gcgattgaac aagcgcaaga
    33061 gaaaatggaa ggtgcagttt tagctagcga tgcttatttt ccaatggatg attcggttga
    33121 atatgcagcc aaacatggta taaaagcaat cattcagcca ggaggaagca tcaaagacca
    33181 agattctatt gatatggcga ataaatacga tctagcaatg gtctttactg atgttcggca
    33241 ttttagacat tagagaggag tcggataaat gaacattttg gtgatcggtg caggtggtcg
    33301 tgaacatgca ataagtaaaa aacttttagc cagtccgaag gtggatacgg tgtattgtgc
    33361 gccaggaaat ccggggatga caaaagatgg tatcaattgt gtcgctatca gtgagcgaga
    33421 tcattcagcg ttaatccatt ttgttaagaa agaggctatt gattggagta tcgttggtcc
    33481 tgaagttcct ttgttaaatg ggatcgtcga tgattttatg gcggaaggat tgaaaatctt
    33541 cggtccgaca aaagcagcag ctctgatcga aggttcgaaa agttttgcta aacaattaat
    33601 ggatacttat caaatcccga cagctaaatc cagaagtttt gaatgttacg aaaaagcagc
    33661 agcgtatatc aaagaaaatg gtgcgccaat cgtagtgaaa gcggacggct tagcagctgg
    33721 gaaaggtgta gtcgtggcta ccacagtgaa ggaagcgctg gatgcggcag aacaaatgtt
    33781 gaaccagcat cgatttgggg atagtagcaa aaaaatcgtc gtagaagagt ttttggcagg
    33841 agaagaattc tcccttttgg cgtttgtccg taataccaag gtgtatccga tggtcatttc
    33901 gcaagatcat aagcgggcat ttgataacga taaagggcca aatacaggcg gaatgggtgc
    33961 ttattcacca gtaccgcaaa tttcaaaaaa gatgattgaa aaagctgtgg aagaagtttt
    34021 aaaaccagcg gctttgggaa tgaaagaact aggacgctct ttcacgggtg ttttatatgc
    34081 gggtttgatt gctacgaagg aaggaccaaa agtgatcgaa ttcaatgcgc gtttcggtga
    34141 tcctgaaacg caagtcgttc tttctcggtt gaagagtgac tttgcgcaag taatcgatga
    34201 tttattagag aatcgtacag tagagttaaa gtggcagcaa gaaggataca atgtaggcgt
    34261 tgtggttgca gctgccggtt accccgaaga atatgagaca ggaatggttc taccagattt
    34321 tctggaagaa gagctttctg tttactatgc tggagtttcc tcaaataacg gcaaactagt
    34381 tggagcagga ggacgtcttt atttagtaga agcatacggt gagacgatca aagaagcaca
    34441 gaaaaaaatt tacaccgcgt taatggataa aacgacagat ggaacatttt atcgtacaga
    34501 tattggagaa aaagctgcca atagcgaact gatcgtgaag taaatcagct gcttccattt
    34561 ataaaaaata caacaaacat aatcggaagc gccgatttta gtgaagcctt ttcataaaga
    34621 gtgtggtaca cttttgttgt gtatataaat aaggaggagt tcagatgaaa aaaattcaat
    34681 taggtacttc tgatcagaaa gcagctgcag tgatcttagg ctgtatgcgt atcaacaatg
    34741 cggaagatcc agtaaaagtg attgagacag cttatgaaaa cggcattgat ttttttgacc
    34801 atgcagatat ttatggaaac ggtgaatgtg aagaaatctt tgcagatgct ctagcaaaaa
    34861 catcaatcaa acgagaagat ctttttattc aaacaaaatg tggaatcgtt ccaggtgtga
    34921 tgtttgattt ttctaaaaaa catatcattg aatctgtaga aaaaagtttg acccgtctga
    34981 aaatggatta tgtggatgct ttacttcttc atcgtccaga tacattggta gaaccagagg
    35041 aagtagctga agcgtttgat gaattggaac ggtcaggaaa agttcgttat tttggcgtca
    35101 gcaatcaaaa accaatgcaa atcgaattgc tgaaaaaata tgttcgtcaa ccattagtag
    35161 ctaatcagct gcaatttggc ttgaaacata ctggaatgat cgatcaaggg attcatgtca
    35221 atatgacaga tgaagcaagt atcgatcatg acggtagtgt acttgattat tcccgattgc
    35281 atgatatgac gatccaagca tggtcaccat accagtatgg attttttgaa ggtgtattta
    35341 tcgggaacga aaaattccct gaattaaacc aaacattaga agaactagca gctaaatatc
    35401 aaacgacacc aactggcttg gcttctgctt ggattttacg ccatccagcc aatatgcaag
    35461 tgatcgccgg tacgatgaac gtagggcgaa tcaaggaaat cgctcaagct tcagaaatta
    35521 atatgtctcg ggaagattgg tacgaattgt accgtgctgc tggaaacatt ttaccttaaa
    35581 gattgtcata agcaggtcaa aagggctata aattaaaaag acagaataac ttgaaagctg
    35641 ttttcagctt cgagatattc tgtctttttt tattgcaaat ggaaagagta tcattgtacg
    35701 gatttcgttt attagttatt tttgttttta aggaaaagct gtgtccgata ttcttttgga
    35761 gaaatccctt gttttttctt aaataaataa ctgaaatagt tagggtcatt ataaccaatt
    35821 tcaaaagcaa tatctgaaat acgatagtct gattgctgca ataatttttt tgctaattcg
    35881 attctttgtt ctgtcagata atcgatgaat gtttggttaa acgattgaga aaaaatcgta
    35941 ctgaaatgag agggacttaa tgcaacttgt tgagcgaccc aagttaagga catatctgga
    36001 tcggtaaaat tttctttgat aaaatccaat gcatggttca aaattggctg atatttgttc
    36061 cttgaaggct gtgagggcag agcagttaag aaagaaatga acatataaac tgtcgaacgg
    36121 taaagcgagt cgtcggcgct gattgctaag cgttgactgg tggatgtgca atgaaccatt
    36181 tcttgcggga ttttcttttc tttcaggcag acatgctgga gtagcttagt caagacaaat
    36241 aaacgatata atcgtgttcg agttggtgtg ccttgtggtt tcgtcaagtg ttcgatgtac
    36301 ttagatactt cgtttttcga taatgaagac aaatcaagtg agaggtcaaa gtaaaagaca
    36361 gaatcaactg gaagtttcgc ttcttgaatc aaatcctcat agctgataat ttgttcttta
    36421 agggaactgg cggtttcaag tatcatctgc gtgatctcaa aagatgcagg taattcgctg
    36481 attctttctg cgacaggacc aatggcgaca gaaaaattat ttttttgtcc cttttctaat
    36541 tcatggacca acgtttgggc tacctgataa ctggtctcga tcacattttc ttcaagagaa
    36601 tcaactatta aaaatttgat gaatcgagag gaaacagcag agaagatcac ttgttgattc
    36661 tccctaaata aaaacttcaa ataatcactg agatgagcgt aatcttcaaa gtctgtactg
    36721 acttggtttg ctgctaataa tacggtcatt tttttccctg ctaaagagcg attgaaagat
    36781 tcagcaagtt tcaatgtttc actgatcgta agtgagcctt tgaacaaacc gttcaagaaa
    36841 tgattttttt tcaattcgaa aacaagatca gaagaagtgt gcgtttcttt ttgctgcaaa
    36901 tctaaagcag ttactgcttt ttctattgct tgtttcaatt cgctactttt aatcggttta
    36961 agcagataat catctgcctg tacctggatt gctcctttca tataatcgaa atcgtcgaat
    37021 ccactaataa atatgagacg agtccaagga agtagtttcc ttgcttcttt tgaaaaagtc
    37081 aacccgtcca tgaaaggcat ttggatatca gtcagaatga tgtcgggctg taagtcgata
    37141 attgaagaca gtgccatctc accatctcca gcttcgccga cataagtgat tggataataa
    37201 gaaccaagga cagttatttg ttcccgcaag ttattgcgaa tgaggtattc gtcttcgatg
    37261 ataaaaactt tatacatgtt tgtttcctcc aattgttttg ggagatagag ggatcatcag
    37321 tgtgacagtt gttccttctc ggtatttgct gtcaatcgtg ataggttttt gatcatttcc
    37381 gtaataaaga aggagacgct tgtttacgtt gtaaagtccg tagccgatac tgaaatctga
    37441 atcgatccct ttggctaact ctttacgtat ttctgccagt ttttctgcag ggatgccaat
    37501 cccgttgtct ataactttga attggatcat ctcatcaatt ttgcatgcgg taatacgaat
    37561 aaatcctttg tgacggacgt acttcgttcc atggtataca gcattttcaa tcaatggctg
    37621 caagatcatt tttaaaatag aaacaggaaa aagtgtttct ggtatatcga tcttatagtc
    37681 aagcatatgg ccgtatctta tttttaagat gactagataa tcattgatat ggctaatttc
    37741 tttttcaaga ggaatccagt cttgtccttt gcttaaagac gtacgaaaaa agccggaaag
    37801 tgcatgggtc atagtcagag cttcttcaaa gcagtcttgt tcgatcaaag aaataatggc
    37861 atccaaactg ttataaataa aatggggagt aatctgagct tgcaatacac gaacttcact
    37921 ttgtgctaaa tggtattgtt tcaaagcgtt ctcccgtaaa gactggttta gatcttccgc
    37981 cattttattc atgctagcgg taagtgtttg taattccagt gtttccggca gggttaaacg
    38041 aaagccaagg ttccctgaag aaagttgttc tgacatctcg atcagctgat tgatcggttc
    38101 ttggaccttt ctctctaaaa agcgacgatt ctttttcgca tagaaaaaaa tggtactaag
    38161 aactccaagt tccactattg ttaatagaat gagcgagcgg acaagtgtcg tatttttttt
    38221 gcttgctaag tcgatctcca cacgaacaaa atcttgtaag atatctgaca gcagttgagt
    38281 gacagaatct acctgattga tgatctcttc attggtatcc ataaaatctt ttgccaagat
    38341 attattgatc agctcgtttt gataatcttc cagtgtatcg atcgtacgta gcgatacatc
    38401 taacgtacta atttctttaa gtgtatttgt attatttttg atctctcgaa tatcattcct
    38461 aagttcttct aggatatttt cctcattgaa ctcatctacg gttacttggc cagaaatcaa
    38521 gtcccacata ctttccaaaa ctttttgatc aaccttcaaa gcaattgcat ctgctttttg
    38581 gatatttgcg atcgtctgtt gataaatcaa aagctgttgc gaatataaca gtatgctcag
    38641 tagcaaaggg ataacagcaa atagaagcat ggctttgttt gcttgggcaa aaatctgttt
    38701 tatgcttaaa ttttttgtca tgatacttct ccagtcaata atttctggtt gggatgttag
    38761 ctatattctg ctgggaaaag acagtctcgg aaataaaaat atctttaggt aatgtttttc
    38821 ctgtttgaat atttccaaaa taacgagtaa cggtattggc tacataccag ccgacatcag
    38881 gtttacactc gactacacaa ttgacgattc ctgagcgtag cttctcgatc atatcttctt
    38941 gtccatcaat agttacaatg atcaaatctt ctccaggttt gattgatgtt tcttttatcg
    39001 cagctaaagc accgtgagtc atttcatcac tatggctgta taatacatcg atttcggata
    39061 aatcatttgt ttctaaatat cgtttcatct ggtcttttcc tttcatccga ataaaatcac
    39121 cgacaattcg gtgaacaata gtcatcggag gtttatggtc gatggttcca tcttttttta
    39181 gggttggttt acgattgatt gattccaaaa atccttcgga gcgtagcttg gtcggtgtgg
    39241 agttctctga accagcaagt tctaggattc tgatttcctg tttctttgaa tttgtgaagt
    39301 gattgctgac aaataaaccc gcccgatttc cttctgcttt gaaactgggg ccaatgtggg
    39361 tgacgaaaag ctcgggatca ttcgtttgaa tatgtctgtc gacaagtatg acagggatcc
    39421 ctgctcgctt tgcttctttt aatacagagt cccacccgga ttcttgcaaa ggagtaaaaa
    39481 tgatcatatc cactttatag gcaatgaacg tccgtatatc ttggatttgc cgttcctgat
    39541 tcatgtagcc atttcggtag agaacttggt aatcttcttt ttcaagcgca gtgataattg
    39601 attcagtatg ctttttccgc cagctactct ccgttcctga ttgcgaaaaa ccaacgacta
    39661 gtgggtcagg tgcttccttt gtattaggtg tacaccccat catttggtaa gcgattacaa
    39721 caagggctaa aaaaaataaa acacgtttca ctttaaatca ctccatctgg atttgttagt
    39781 actatcttat cagataaaag ggaaaatcaa agtcaaaagg aatcaatcga aaaaaatcca
    39841 agaaacttaa aaaaatccaa gacaaaaaaa gtacacctaa aaaatttaaa gtaaatccca
    39901 taaaaaaatc aatgttaacg ctttctttat tagctatact cggtgtatca attgaaatgg
    39961 gggataaaaa atgaaaaaca aatttagaaa gattgttggg actatcgcta ttttgaccgc
    40021 aagcattcta ttaagtgcgt gtggcaattc aggatctgca gatgatagta cggggtatgt
    40081 aggaatcgct atgccgacca aatcagcaga aagatggatc gctgatggga acaacatggt
    40141 ctcggaatta gaaaaactgg gttataaaac agatcttcaa tatggagaag ataaagtaga
    40201 aaatcaagta gcacaaatag aaaatatgat tacaaaaggg gtagatacac ttgtgattgc
    40261 ttctatcgat ggttcggctt taactgatgt gttagctaaa gcaaaagaag cagatatcaa
    40321 ggtgatcgcc tatgatcgtt tactgatgaa ttcagaaaat gtcgattatt acgcgacttt
    40381 tgataatttt ggagtaggtg tatcgcaagc agcttatatt gaagagcact taggattgaa
    40441 agaaggaaaa ggaccattca cgattgaatt atttggtggc tcgcctgacg acaacaacgc
    40501 actaatcaat tacaacggag tgatgtctgt tttacaacca tatatggaca acggtcagtt
    40561 ggtcgtgcca tctggacaga cgagtttcag tcaaatcgct acgcttcgct gggatggttc
    40621 tacggcgcaa gcaaggatgg ataatctgtt aagcgcaaat tatactgacc aaacattgga
    40681 tgctgttttg tcgccatatg atccaataag tttaggaatc atttcgtcat taaaaggcgt
    40741 ggggtatggg agtgaaagta aacctttacc tgtgattact ggacaagatg cgacagttgc
    40801 tggcgttaaa tcgatcattg ccggggaaca aacacaaacg atctttaaag acacacgtat
    40861 cttagcaaaa aatacgattg aaatgatcaa agcaatcagt gatggagaag aggttccggt
    40921 caatgatact gaaacttatg ataatggggt gaaaactgtc cctacgtatt tagcaaatac
    40981 agtatctgtc gataaagata attatcaagc agagctgatc gacaccgatt attataaaga
    41041 atccgatctg aaaaattaag aggaaccaaa caaggaacta aagagatcta agtcagagag
    41101 aggataagaa ttatggcgga ttgtatttta aaaatggcag atatcaccaa aaaattttct
    41161 ggaatccgag cgttagattg cgtgaatctg atgattgaac gtggggaaat ccatgcattg
    41221 tgcggagaaa acggagcggg aaaatcaaca ttgatgaatg tcttgagcgg cttgtatcca
    41281 tatggctcct atgacgggga aatcttttat gatggaaagc tgtgtcaatt taaagatttg
    41341 aaagatagtg aagaaaaagg catcgtcatc attcatcaag aattagcact aagtccttat
    41401 ctttcaataa aagagaatat ttttctcgga aatgaacaag cagaaagagg cgtgattgat
    41461 tgggaactga cagaaaagaa aacatatgaa ctgctgaata aagtcggttt gtctatcgac
    41521 ccgaatcttc tagtatccca gattggcgta gggcaacagc aactagttga aatagctaaa
    41581 gcattatcaa aatccgtccg tctgttgatt ttggatgagc ctaccgctgc attgaatgaa
    41641 gaagaaagcg cgaatttgct gagattgatc cgagagttga aagagcaagg tgtaacctcc
    41701 atcattattt cacataagct gaatgaaatc gtggatatcg cagatcgaat caccatttta
    41761 cgggatggac agacgattga gacactagaa aatgaaatga tcagcgaaga gcatattatt
    41821 cgcgggatgg tgggcagaga tttagttgat cgttatcctg aaagaaaacc ggatattgga
    41881 gaagtttatt ttaaagtaga agactggacg gtctatcacc ctcttgatca cgagcgaatc
    41941 ataaacgacc acatcaactt gcatttgaaa aaaggagaga ttgtaggaat agctggactg
    42001 atgggagcgg gcagaactga attcgctatg agtctatttg gacattctta tggaagcaag
    42061 atatccggac gtatattaaa ggaaggggca gagatatcag ttaagaccgt tccttcagcc
    42121 attgcaaacg gactcgctta tgtctcggag gaccgaaaat ctttaggact gaatctgctg
    42181 atggatattc gggaaaacgt gacgctgagc agtttggata aaataagtca aaaaggagta
    42241 ttagataaag aaaaagaagt aactgaggca gaaaatttcc ggaaaaagat gcgtataaaa
    42301 acaaactcca tccatcaaat tgtcagtagt cttagtggtg ggaatcaaca aaaagtcgtt
    42361 ttatccaagt ggctgatgac acaacccgat attctttttt tggatgagcc aacccgtggg
    42421 atagatgttg gggcaaaata tgagatctac acgattatcg aagaaatggc agcaaatggc
    42481 aaagctgtct gtcttatctc ttctgaactt cctgaaatat tagggatgtg tgatcgaatc
    42541 tatacgatga acgaaggacg tattacagga gaagtctcgc gagaagatgc agatcaggaa
    42601 gtcttgatga aattaatgac gaaagaagag gtgggttaag ttggaacagg caaaaaatct
    42661 tacggataaa aaagagaaaa aactaaattt gaaaagtaga gcagctgatc tttttggtcg
    42721 ctacagcatg gtgatcattt tagcgggact acttattgcg tttcaattga tgacggatgg
    42781 tattttctgg cgtccattga atataactaa ccttgttctg caaaatagcc atatattagt
    42841 actagctgca gggatgttat tagttgtgct cttaggtcat gtggatttgt cggtagggtc
    42901 agtgatggcg tttgttggtg caattgcggg catgatgatg gtcaatcgcc aaatgaaccc
    42961 gtggatgaca gttatcgcat gtcttgcagt aggcgggctg atcggcgcat ggcaaggttt
    43021 ttgggtagct tatgcaggga taccggcgtt tatcgtgact ttagcaggat tgctgatgtt
    43081 tcgtggactg acacaagttg ttctaggagg ccaatctcta gcaccttttc cagctgtttt
    43141 ccaaaaaatg tctacgggtt atttaccaga cttaagcgaa ggaagcagcc ttcacttctt
    43201 gacattgatt ttaggaatga tcatcacgat cgtgttagtt gttggtcaat ggcgaacaag
    43261 aaatcgtcgg aaaaatcatt tatttgaagt ggaatctcca acagctttct tggtaaaggc
    43321 ggtatttaca gcagtcgttg tactaggaat aacttatatc tttgcctctt atcaagggta
    43381 tccgattatt ttgatcattc ttggtttgat cgttgcagca tatgggtttt tgacaaataa
    43441 aacagtagca ggtcgacaga tttatgcgac gggcggaaat cgaaaagcgg ctgaactatc
    43501 agggatcaaa acaaagcgaa tcacgttctg ggtattcgtc aacatgggag tcatggcagc
    43561 tttagctgga ttagttttag ctgctcgatt gaatgcggca acacctcaag caggtacatc
    43621 gatggaactg gatgcaatgg cggcagtata ttttggtggt gcttcgactt ccggcggcat
    43681 tggaacaatc atgggtgcga ttgtcggtgg cttggtcatg ggtgtattga acaatggcat
    43741 gtcgatttta ggcgtcgggg tagactggca gcaggcaatc aaaggattga tcttgctttt
    43801 agccgttgtg ctagacttgt ataataagaa aaaacgaaca acgtaggaga acgccggtta
    43861 ttcagaaaaa agactgtgac aagacatttg tcacagtctt tttttcgcta aaagcgaaca
    43921 ttcattgttt cgtttgtata gactaaaaat aatatatgtt tttttaccat tcgtataaat
    43981 tctctttttg ctcttgattt ttttgcttaa ttgagttaaa ttacataggg cgctaaaaaa
    44041 agccttgatt aaattaataa tgttcgtgtt ttgctggatg tgaacatcag gaggataatg
    44101 taatgaaagt atttgattac gaagatattc aattaattcc taataaatgt atcgtaaata
    44161 gccgttctga atgtgataca acggtcacat taggtaaaca tacattcaaa atgccagtag
    44221 tgcctgccaa catgcagacg attattgatg aaacaatcgc agaattctta gcggaaaatg
    44281 gttatttcta tatcatgcat cggtttgatg aagcagctcg aattccgttt atcaaaaaaa
    44341 tgaaaaagcg tggattgatt acctcgatca gtgttggcgt caagaaagag gaatattcct
    44401 ttatagagaa gttggcagaa gaatcgctaa atcctgatta tatcactatc gacatcgcgc
    44461 atggacatgc taactcagtt attgatatga ttcagcatat taaaaaatat ttgcctgaaa
    44521 cttttgtgat tgccggtaat gttggtacac cggaagccgt ccgtgaattg gaaaatgcgg
    44581 gcgcagatgc aacaaaagtc gggattggtc caggaaaagt atgtattaca aaaatcaaga
    44641 caggctttgg gactggaggc tggcaactgg cagctcttag atggtgtgcg aaagcagctc
    44701 gcaagccgat catcgctgac ggtgggatca gaacacatgg cgatattgca aaatctgtcc
    44761 gttttggtgc gacaatggtc atgattggtt cattgtttgc cggtcacgag gaatctcctg
    44821 gcgaaacaaa agtcgaaaat ggaatcgtgt ataaagaata ctttggttct gcttcagaat
    44881 tccaaaaagg cgaaaaacga aatgttgaag gaaagaaaat ctggatccaa cacaaaggat
    44941 ctttgaaaga tacactagtg gaaatgcaac aagaccttca atcatctatt tcttatgctg
    45001 gaggaagaga tctggaagcc attcgaaaag ttgactatgt catcgtgaaa aattcgattt
    45061 ttaatggaga cacaatctaa aaaataaata aaatattagg atgttgtgaa aaagttgtcc
    45121 tgcat
//
LOCUS       DARDHA010000007        44917 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000007 DARDHA010000000
VERSION     DARDHA010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.007"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            212..604
                     /locus_tag="SDA10_000311"
     CDS             212..604
                     /locus_tag="SDA10_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG4239243.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="SDA10_000312"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="SDA10_000312"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEG4239244.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="SDA10_000313"
     CDS             3165..4775
                     /locus_tag="SDA10_000313"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEG4239245.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="SDA10_000314"
     CDS             5067..5936
                     /locus_tag="SDA10_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEG4239246.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="SDA10_000315"
     CDS             6101..7372
                     /locus_tag="SDA10_000315"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEG4239247.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="SDA10_000316"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="SDA10_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEG4239248.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="SDA10_000317"
     CDS             8763..9026
                     /locus_tag="SDA10_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEG4239249.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="SDA10_000318"
     CDS             9471..9920
                     /locus_tag="SDA10_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4239250.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="SDA10_000319"
     CDS             9977..11395
                     /locus_tag="SDA10_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4239251.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="SDA10_000320"
     CDS             11395..12255
                     /locus_tag="SDA10_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEG4239252.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="SDA10_000321"
     CDS             12429..13202
                     /locus_tag="SDA10_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG4239253.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="SDA10_000322"
     CDS             13349..13714
                     /locus_tag="SDA10_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HEG4239254.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="SDA10_000323"
     CDS             complement(13862..15070)
                     /locus_tag="SDA10_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4239255.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="SDA10_000324"
     CDS             complement(15089..15367)
                     /locus_tag="SDA10_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239256.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="SDA10_000325"
     CDS             15521..16216
                     /locus_tag="SDA10_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG4239257.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="SDA10_000326"
     CDS             complement(16658..17455)
                     /locus_tag="SDA10_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEG4239258.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="SDA10_000327"
     CDS             complement(17452..18636)
                     /locus_tag="SDA10_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEG4239259.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="SDA10_000328"
     CDS             complement(18661..19908)
                     /locus_tag="SDA10_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEG4239260.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="SDA10_000329"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="SDA10_000329"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEG4239261.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="SDA10_000330"
     CDS             21888..22202
                     /locus_tag="SDA10_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEG4239262.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="SDA10_000331"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="SDA10_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEG4239263.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="SDA10_000332"
     CDS             22887..23531
                     /locus_tag="SDA10_000332"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEG4239264.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="SDA10_000333"
     CDS             23546..23875
                     /locus_tag="SDA10_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEG4239265.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="SDA10_000334"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="SDA10_000334"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEG4239266.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="SDA10_000335"
     CDS             24863..25687
                     /locus_tag="SDA10_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEG4239267.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="SDA10_000336"
     CDS             25680..26027
                     /locus_tag="SDA10_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEG4239268.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="SDA10_000337"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="SDA10_000337"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEG4239269.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="SDA10_000338"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="SDA10_000338"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEG4239270.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="SDA10_000339"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="SDA10_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEG4239271.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="SDA10_000340"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="SDA10_000340"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEG4239272.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="SDA10_000341"
     CDS             31657..32451
                     /locus_tag="SDA10_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4239273.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="SDA10_000342"
     CDS             32441..34483
                     /locus_tag="SDA10_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4239274.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="SDA10_000343"
     CDS             34764..34913
                     /locus_tag="SDA10_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEG4239275.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="SDA10_000344"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="SDA10_000344"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEG4239276.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="SDA10_000345"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="SDA10_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEG4239277.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="SDA10_000346"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="SDA10_000346"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEG4239278.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="SDA10_000347"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="SDA10_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEG4239279.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="SDA10_000348"
     CDS             39330..40412
                     /locus_tag="SDA10_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEG4239280.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="SDA10_000349"
     CDS             40412..41215
                     /locus_tag="SDA10_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEG4239281.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="SDA10_000350"
     CDS             41285..42025
                     /locus_tag="SDA10_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG4239282.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="SDA10_000351"
     CDS             42036..43040
                     /locus_tag="SDA10_000351"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEG4239283.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44848)
                     /locus_tag="SDA10_000352"
     CDS             complement(43085..44848)
                     /locus_tag="SDA10_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239284.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14441 a         7833 c         9346 g        13297 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    44881 gtaaataaaa agattctttt ttattatata tgttatt
//
LOCUS       DARDHA010000008        44766 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000008 DARDHA010000000
VERSION     DARDHA010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.008"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..2377)
                     /gene="mprF"
                     /locus_tag="SDA10_000353"
     CDS             complement(<1..2377)
                     /gene="mprF"
                     /locus_tag="SDA10_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEG4239285.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVY"
     gene            2518..3324
                     /gene="proC"
                     /locus_tag="SDA10_000354"
     CDS             2518..3324
                     /gene="proC"
                     /locus_tag="SDA10_000354"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEG4239286.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(3403..4959)
                     /gene="rny"
                     /locus_tag="SDA10_000355"
     CDS             complement(3403..4959)
                     /gene="rny"
                     /locus_tag="SDA10_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEG4239287.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(5370..6419)
                     /gene="recA"
                     /locus_tag="SDA10_000356"
     CDS             complement(5370..6419)
                     /gene="recA"
                     /locus_tag="SDA10_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEG4239288.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(6528..7769)
                     /locus_tag="SDA10_000357"
     CDS             complement(6528..7769)
                     /locus_tag="SDA10_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEG4239289.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(8009..8587)
                     /gene="pgsA"
                     /locus_tag="SDA10_000358"
     CDS             complement(8009..8587)
                     /gene="pgsA"
                     /locus_tag="SDA10_000358"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEG4239290.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(8796..9719)
                     /locus_tag="SDA10_000359"
     CDS             complement(8796..9719)
                     /locus_tag="SDA10_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4239291.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(10458..11744)
                     /locus_tag="SDA10_000360"
     CDS             complement(10458..11744)
                     /locus_tag="SDA10_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEG4239292.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(11737..13005)
                     /locus_tag="SDA10_000361"
     CDS             complement(11737..13005)
                     /locus_tag="SDA10_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEG4239293.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            13257..13718
                     /gene="mscL"
                     /locus_tag="SDA10_000362"
     CDS             13257..13718
                     /gene="mscL"
                     /locus_tag="SDA10_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEG4239294.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(13778..14224)
                     /locus_tag="SDA10_000363"
     CDS             complement(13778..14224)
                     /locus_tag="SDA10_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4239295.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(14317..14562)
                     /locus_tag="SDA10_000364"
     CDS             complement(14317..14562)
                     /locus_tag="SDA10_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEG4239296.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(14677..15255)
                     /locus_tag="SDA10_000365"
     CDS             complement(14677..15255)
                     /locus_tag="SDA10_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG4239297.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(15332..15862)
                     /locus_tag="SDA10_000366"
     CDS             complement(15332..15862)
                     /locus_tag="SDA10_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEG4239298.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(15866..16726)
                     /gene="rlmB"
                     /locus_tag="SDA10_000367"
     CDS             complement(15866..16726)
                     /gene="rlmB"
                     /locus_tag="SDA10_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEG4239299.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(16719..17114)
                     /locus_tag="SDA10_000368"
     CDS             complement(16719..17114)
                     /locus_tag="SDA10_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEG4239300.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(17111..18523)
                     /locus_tag="SDA10_000369"
     CDS             complement(17111..18523)
                     /locus_tag="SDA10_000369"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEG4239301.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(18520..19056)
                     /gene="cysE"
                     /locus_tag="SDA10_000370"
     CDS             complement(18520..19056)
                     /gene="cysE"
                     /locus_tag="SDA10_000370"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEG4239302.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(19142..19381)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(19378..20838)
                     /locus_tag="SDA10_000371"
     CDS             complement(19378..20838)
                     /locus_tag="SDA10_000371"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEG4239303.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(21008..22165)
                     /locus_tag="SDA10_000372"
     CDS             complement(21008..22165)
                     /locus_tag="SDA10_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEG4239304.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(22199..23569)
                     /gene="radA"
                     /locus_tag="SDA10_000373"
     CDS             complement(22199..23569)
                     /gene="radA"
                     /locus_tag="SDA10_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEG4239305.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(23632..24159)
                     /locus_tag="SDA10_000374"
     CDS             complement(23632..24159)
                     /locus_tag="SDA10_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEG4239306.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            24389..25195
                     /gene="proB"
                     /locus_tag="SDA10_000375"
     CDS             24389..25195
                     /gene="proB"
                     /locus_tag="SDA10_000375"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEG4239307.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            25192..26439
                     /locus_tag="SDA10_000376"
     CDS             25192..26439
                     /locus_tag="SDA10_000376"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEG4239308.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            26515..26859
                     /locus_tag="SDA10_000377"
     CDS             26515..26859
                     /locus_tag="SDA10_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239309.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(26976..27302)
                     /locus_tag="SDA10_000378"
     CDS             complement(26976..27302)
                     /locus_tag="SDA10_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239310.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(27917..29119)
                     /locus_tag="SDA10_000379"
     CDS             complement(27917..29119)
                     /locus_tag="SDA10_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4239311.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            29236..29751
                     /locus_tag="SDA10_000380"
     CDS             29236..29751
                     /locus_tag="SDA10_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG4239312.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(29950..30396)
                     /locus_tag="SDA10_000381"
     CDS             complement(29950..30396)
                     /locus_tag="SDA10_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEG4239313.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(30443..31921)
                     /gene="msr(C)"
                     /locus_tag="SDA10_000382"
     CDS             complement(30443..31921)
                     /gene="msr(C)"
                     /locus_tag="SDA10_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEG4239314.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(32275..32904)
                     /locus_tag="SDA10_000383"
     CDS             complement(32275..32904)
                     /locus_tag="SDA10_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG4239315.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(32888..33352)
                     /locus_tag="SDA10_000384"
     CDS             complement(32888..33352)
                     /locus_tag="SDA10_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG4239316.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(33839..35107)
                     /locus_tag="SDA10_000385"
     CDS             complement(33839..35107)
                     /locus_tag="SDA10_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEG4239317.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(35338..36075)
                     /locus_tag="SDA10_000386"
     CDS             complement(35338..36075)
                     /locus_tag="SDA10_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG4239318.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(36142..36711)
                     /locus_tag="SDA10_000387"
     CDS             complement(36142..36711)
                     /locus_tag="SDA10_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEG4239319.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            36839..37711
                     /locus_tag="SDA10_000388"
     CDS             36839..37711
                     /locus_tag="SDA10_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG4239320.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            38053..38925
                     /locus_tag="SDA10_000389"
     CDS             38053..38925
                     /locus_tag="SDA10_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG4239321.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            38922..40880
                     /locus_tag="SDA10_000390"
     CDS             38922..40880
                     /locus_tag="SDA10_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4239322.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(41212..42063)
                     /locus_tag="SDA10_000391"
     CDS             complement(41212..42063)
                     /locus_tag="SDA10_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG4239323.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            42186..42653
                     /locus_tag="SDA10_000392"
     CDS             42186..42653
                     /locus_tag="SDA10_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEG4239324.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(42916..43398)
                     /locus_tag="SDA10_000393"
     CDS             complement(42916..43398)
                     /locus_tag="SDA10_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEG4239325.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(43498..44361)
                     /locus_tag="SDA10_000394"
     CDS             complement(43498..44361)
                     /locus_tag="SDA10_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEG4239326.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        12785 a         9544 c         8047 g        14390 t
ORIGIN      
        1 tatatacgat atgcatgact ttttcttgaa agaaagaaaa atctgtttct ccaacattag
       61 ctaatggagc taagcctaga ttggcttccg agtagcccat tttctcgtat acaataaaca
      121 gattggctaa cagatagtca gctaattcct cagattcttt aggtaagacc cgcaacaaat
      181 catatcccgc ctgcttcttc gtcatcggct gagcggtgat gaagccaacg actttttgat
      241 ctttacggac aataccgaca ccgctagata atacatatga tttatcaaat cgtcccacag
      301 agaagtattt ttctcgtgca ccttgaagcc atttttgtga aacttcattt acagcttgat
      361 gtaattcttc cggcagttga tcgtaccaag taaaatggta tccttctttc atcaaacgtt
      421 gatattcaag ttgctgactc agccgagaca agtcttctgt tgctagatca acaatccctg
      481 cttcacctac tttcgtgaag tgaaaaccca attcatgaag catcactacg tagctttctg
      541 atatcttata aaacgcaagc tgatagccta aacaatctgc ttgtttcata aactgattcg
      601 ttgctgcccg catcatttta ggattgccaa ctggatctcc taaaacaaag catttgtttc
      661 ctttgatctg ataaccaaaa acgacttgat cttggtcgtc ttcttggtaa taaaaataac
      721 tgtaacctga caagcgaagc tgatggcttg tagctgtgcc tccgtatgtt tccaataaac
      781 ggtcaaaacg gtctttttcg tactcactgc caagcttcgt agtatcttca gctaaatatt
      841 gataaagcgc cactaaagct agcaatgaga tgcccaaacc aataagccct gagaaccaca
      901 catcatctga aggaaacaaa ataaaatgat ttcctgttat tcgaccgact aaaaagcgtc
      961 cgctgtgata gcctgcaatt gcgtaaacga taaacaaaaa gccgaacaag ctagcatcaa
     1021 agattatagc tccccaagaa taaacgaatt ttgttcgata aaattctttt ctggataacc
     1081 acacagctaa cagaatcaag acatatacca atatcaactg ccaagataac gtacgtgtga
     1141 ttgtatttaa aataccaaaa gatagtaaag caatcgtagg cagaaatgcc tttttgactt
     1201 ttactgaaag tgctctagca aggccgagta ataaaaagcc aaccaataga ttcactgtct
     1261 gatctaaaaa attgaatgag aacggcaata atacttgaaa tagccgactg attgcagaca
     1321 gattagtgat tgttgataga agtaccatca tgatccccgc aaaatagaca gcacttacga
     1381 gcacgatatg cgcaaccttt tggaaaaaca gtcgcggaat gttatctaaa aatcgattga
     1441 ttttgactcc tgtatgtgtg atgaacaata gaagtcccgt cataaaaggt agaacataat
     1501 aaaacaaccg ataatataat agccaaacta ctgctacatc ttgccgaaca gacaattgac
     1561 tcattcctaa gatcatcaaa acgtcaaaag tccccattcc acctgggacc atcgtcagca
     1621 ttcctatcaa tgtagcaatc atgaacatag gatagatcga aagcatcgaa atatctattc
     1681 tcatcaactt gccgatgatc aaaaataccg caagggctcc cgtccattgt ccaaaggatg
     1741 ccagtagcag accaagcaag ccttttggat aaaactcgaa gaacaatgtc tttctcttta
     1801 gataagcaaa aaagaaaatg actggtgcta aaaaactacc gccgaaaagc cagatccaat
     1861 agcttcgaaa aatactttct gactgaataa aaaagacatc aataaatgta agaaacgacc
     1921 atacagacaa accagtcagc ataaatagtg cgacttttga tacagtggct aaaaccattt
     1981 ttctatttgt ctgctttcca taaaaactag cccgcagact tgctcctacc acaccaccaa
     2041 aaccggcgag attgttgatc gtgtttgtta cccacgcagc aagaagaaac tcttttcttt
     2101 ccatttttgg ttttccttgt ttttctaaga ctctgatcgt cacccaatca tacaacaaca
     2161 tcggagtgac acccagcagt ccagccagaa tcatgagcag gatcgtcatg cggctttgtt
     2221 gtcccatcct ttgccaaata tcttgccaac tcatgccatt agcaatattt gcgacttgat
     2281 ttgccacaaa gactaacacg gagccgaaaa aaatcagttt taaaatcaga ctatgttcct
     2341 ttatccattg cagcatcaag tatcgtttat ttttcacatt ttcccattcc taacttgtat
     2401 acttttgtgt cataccccaa tgttatctta tttattttaa tttttccact ttagacctgt
     2461 tctttgcttg cgatgataga aaaagaaaga tatcctaatg ctaaaggaga ggataacatg
     2521 aaagtaggat ttatcggcac agggaatatg gcacaagcta ttattgatgg tttattaaaa
     2581 aaaggaacag tctcgaaaga agaggtgttc gtttccagtg gacattttga aaatgcacaa
     2641 aaatttgctg ctgaaactgg cgtccatgca tgcaaaagta atcaggaagt tgcatcacaa
     2701 tcagatatcg ttcttttagc cgtgaaacca aaaatcattg aaaaagtatt ggaagaacta
     2761 agagaatata gccagaaagt cttattcatt tctattgctt caggaaaatc attaatagat
     2821 ttagaaacag cgcttgaaga tcctcaggcc tccatcgtac gtgtgatgcc gaatgtcaat
     2881 gtcagcgtag gagcaggcgt ttctgcgatt tgcaaaaatg aagctgccac ggaggaacag
     2941 cttcagcaag ctacgatgat ttttgaagca atcggcagcg tctatccttt agcagaatca
     3001 gatttcagta cattcattgg ccttgctggc agctcacccg cattcgttta catgctgatc
     3061 gatgcgatgg gacggactgg tgtactgaat ggtttgtcca aacagacagc cactgagatt
     3121 gctgcaaaag caattatggg ttcttgtcaa aaattccttt caagtgataa aaatccatgg
     3181 gaacttgtag accaagtttc ttctcctggc ggtacaactg ttgcaggtgt agttgcctta
     3241 gaagaagcag gattcatccc agcaatcatc aaaggaatca atacgacgat cgagaaagat
     3301 cttgagatgt tgaataaaaa ataatgatag caatgttttt tcgtgcctta caaaaaaaga
     3361 agagctgtgg cttttgccgc ggctcttctt ttgtattttt tattatttag cataatcagt
     3421 tgcacgagtt tcccgaataa cgattacttt gatgtgtcca ggataatcca gttcttcttc
     3481 gattcgttta cgaatatcac gtactaaacg aacagcgtct aaatccgaga tttcttctgg
     3541 cttgaccatt acccgaactt cgcgtccagc ttgaactgcg taacttgatt ctacaccggc
     3601 gaagctgttc gaaatatttt ccagacttct taaacgacgg atgtagtttt ctaatgattc
     3661 acttcgcgca ccaggtcttg ctgctgagag cgcatctgct gcagctacaa ggacagagat
     3721 aacagaagta gcttccacat ctccatgatg agaagcgatc gcgttgatta ctactgggtt
     3781 ttctttgtac ttggcagcta attccgcacc gatctcaacg tgagatcctt caatctcatg
     3841 gtcaagtgct ttcccaatat catggagcaa cccggcacgt tttgctaatt ggatatcttc
     3901 gccaagctct gctgctaaga taccagctaa ttttgcaacc tcgaccgaat gcttcaagac
     3961 gttttgtcca taactagttc tgaatcgcag acgtccgatg atcttgatta gatctggatg
     4021 aagtgtatga gcaccgactt caaacgctgc ctcttccccg tattcgcgaa tacgttcatc
     4081 catttcttta cgagatttct cgaccatttc ttcgatacgc gctggatgga ttcgtccatc
     4141 ttgtatcagt ttttccaagg tcatccgtgc gatttctctt cggattggat cgaatccgga
     4201 aagaacaact gcttccggtg tatcatcgat gattaaatcg ataccagtca gcgtctcaag
     4261 cgttcgaata ttacgacctt ctcgtccaat gatacgacct ttcatttcat cattcggaag
     4321 ggtaacgact gaaacagtcg tttctgaaac ttgatctgct gcgcagcgtt gaatcgctaa
     4381 tgataataag tttttcgctt tgcgatctgc ttcttctttg gcacgctgtt cagattcttt
     4441 taccattaga gttaactcat gattcagctc ttcttcagtc gatttcatga tgacatctcg
     4501 tgcgtcctct tttgataaac cagcaatttt ttctagttct ttttgttgat cttcaatcat
     4561 tttttcaacg tctttttctc gctcttcaat taattgctgt tttgaactaa gtgcttcttc
     4621 tttactttca agtgagttct cgcgtttttc taacgagtca tctttacgat tcagcgtttg
     4681 ttcgcgttga atcaaacgat tctcttgaga ttttaattct aacttacttt ctctcagctc
     4741 actttcaact tcagaacggt atttctgatt ttcttcctta gcttctaaca atgcttcttt
     4801 ttttagagtt tctgcttctt tttttgcact atcgatgatt ccagcagctg atgattgtgc
     4861 accagctatt tctttctcgt gacgtgactt tgcgaccata aaccctaaac caagaccgac
     4921 aattaaaccg atgatagcga ggagaatgtt gaataccata aaatccacct ccatactatc
     4981 tttttcattt ttatcatcta tacagcttct atgataaatt attctaaact cgtcaatatg
     5041 catcttcgca tatatgtgta caaaaaacat gcacaacttt attctaatgt ttgtaccccg
     5101 tagtgtcaac tttaaataaa caaagaaacg gctttctact tattcattaa acgatggttt
     5161 ttaaaataaa tgacactagt ttcatatagg taaaccttta aattgagaaa cttttctatt
     5221 aaattattct ttgtagatga acagatattt ttattttatg tacccactcg tataattaaa
     5281 taactcacga tttctttatt caataaaaaa acggcaggtc aaggagagta cctactcttc
     5341 agaccagccg tttttgttta tcataatttt tattcttcta ctaaaggcaa ttcttcttgt
     5401 cctttttcat cttctgggac agcgacttct tcaccaatac cataagctgc tctaactaga
     5461 gcagaaactt ctgccatcat ttcaggatga ttagccatat agattttagc gttttcccgt
     5521 ccttgaccaa tccgatcttc tttataagaa taccaagcac cgcttttatc aacaatgtct
     5581 ttttctactg ccatgtctag aagttctcct tcttgtgaga tccctaaacc atacatgaca
     5641 tctacttcag cgattttgaa aggaggtgct actttgtttt ttacgacttt gatctttgta
     5701 cggttaccaa caatatctgt accttgtttc aattgttctg cacgacgtac ttccaaacgg
     5761 atcgttgcgt agaatttcaa tgcgcgtcct ccaggagtga tttctggatt cccaaacatc
     5821 acgcctactt tttcacggat ttggttgatg aaaatagcga ttgtttttgt tttgttgatc
     5881 gaaccagaga gtttacgcaa tgcttgagac atcaaacgtg cttgtaaccc gacatgtgag
     5941 tcacccattt cgccgtcgat ttctgctcgt ggaactaaag cagcaactga atcgactact
     6001 acgatatcta cagccccact tgatactaaa gcatcagcga tctctagacc ttgttctcct
     6061 gtgtcaggct gtgaaagaag tagttcatcg atattcacac ctaatttttg tgcatattgc
     6121 ggatctaacg catgctcagc atcaatgaaa gcggccgttc cgccattttt ttgtacttct
     6181 gcaatagcgt gtagtgcaac tgttgtttta cctgaacttt caggtccata tacttcgatg
     6241 atacgtccac gggggtaacc gccgacacct aaagcgacat ctaacgcaag agagccactt
     6301 ggaattgtag agatttgttg gtcgattttt tcgcccaatt tcataataga gcctttacca
     6361 tagctctttt caattttttt cagtgcagca tctaaggctg ctttacgatc atctgccaat
     6421 acgataatcc tccttatatt tacaaatcag agcaacattt cattatcctt atcatatctt
     6481 ttttagtgaa aaaaagcaag aaaaaaccga acaaatattc gcatttatca ttttcttaaa
     6541 agctgttttc tcaaaagatc aaatccttgc ataaccgcat tatatctgat agcagcacga
     6601 tctctggcaa agtgatattt ttccgaaacc acgccgtctt tagatgaaat agcgatccat
     6661 actgttcctg cagattgacc ttctaacgtg tccggtccag ccactccggt gaaagctaca
     6721 ctgtaatctg ttcttgctat ctcacgtgct tgaatggcca tctgctccac acattctttg
     6781 ctgactgttc catattctgc taatagctcc ggatcaatct ccagaaaacc agcttttgtt
     6841 tggagcgagt aggtaacgaa tccgcctgga aatacttcag atacaccagc aatgtcgcct
     6901 aatgcggctt ggaaggcgcc ggctgtcaaa ctttccgctg ctgttacagt ctgcttgttt
     6961 tctttcagta gttccactac gacttttgct agcgaattat catcgccgta tccataaaaa
     7021 tattccccta ctcgttcttg gattttttct tctaatgcta aaagagcttg attccctgcc
     7081 tgaacatcat ttgttttcac tgtcagtcgt aatgtcactt catttggttt ggcatatggt
     7141 gcgatcgttg gattgatctg tgtctcaatc agatctttta gctcagtgac cagtcgcgat
     7201 tcaccaatcc cataaaagcg caaaacttta gaaattagtt tttcttcaga aggaaaacgt
     7261 tcctctagta aaggacgtac ttgttccaca aacattggtt tcaactcatt aggcggaccg
     7321 ggaagaagga tatacgcatg tgtatctgtt tgataaaaag tccctaaagc aagccctgta
     7381 cgatttggta gaggaacccc gccttcgata atctgggact gttgcagatt gtttttggtc
     7441 atttttcgat gggttgtttc aaaatatgcc aataattttt tgtatccttc cgtattttgt
     7501 atgagagact taccgaggtg agccgcagtt gcttcttttg tcaggtcatc ttctgtcggt
     7561 cctaatccgc cacataagag tatgagttca cttcttgttt cagctagagc aatcaactct
     7621 tctaaacgct gctgattatc tcctacaact gtttgataat aaacttcgat acctagatca
     7681 gctagctgtt ctgacaagaa agtggcattc gtattcacga cttgtcctag taaaagttct
     7741 gttccgacag ctattatttc tgctttcatt acttcccgct cccttatata gtatacgctg
     7801 tatttttata tttttttatt taaaaagagg gcaaggagct gtgacaaaaa tcttgtcaca
     7861 gctccttgtt ccaaataaac agtgttctcc tgccaaaacc tcggtcataa gtcacaatat
     7921 acataaaaca tgaaaagctg ttttttgaaa ttatcccttg tgactcagag ttagacggca
     7981 ggctcacaac ctctccacta aacgatactt acattgatcc tttgaacaca ccgctgtttt
     8041 tgatgaagta atccacacct gaatagatag taaagatcag acaagcataa agcatgattt
     8101 gatctactgg aataccgatc aggctgaatg ggatattatt gataaacagc aagataatag
     8161 caaccatttg tgtagccgtc ttgacttttc ccggccaagc tgcagccatg acttctccat
     8221 gttcgacaag tagtaaacgc aagcctgtga cggctaattc acgacagaca atgatcgcta
     8281 ctacccaacc aggggccttt ccttggtcaa ccaacatgat aaaagcagtc ataactagca
     8341 ttttatctgc caacgggtcc gcaaacttgc caaaattagt gacaaggcct cgcgcacggg
     8401 caatttttcc atctaaccag tctgtgatac tagctaaagc aaaaataact gcacctacta
     8461 gctgtgtgac agccagtgtg gtgtctccta cgttaaattg tccccaatct aaaggtaaac
     8521 ctacaaccaa aatgaaaatc gggatcatac aaattctaag tacagttaat ttatttggta
     8581 aattcacact atcgactcct ttcttcccta ttctacgtga ctatcagcga agtttccacc
     8641 gatattattt acttttccaa atgaatttat tcaaaggagt atttttattg aaattgatct
     8701 ttttgaatta acgaagaagg accgcgacac agatttgtca cgatccttct tttttatacc
     8761 tacaagtaaa cagatgcgta aactaattta tacgatcatt ctgcgtattg aagagtaagg
     8821 ttcacatttt ttggactctg attgttttcc cctggattaa ctggtacgga ttctccattc
     8881 gctttgatcg ttgcattgct tgctgcacca atcgtgatgg tcgcatttgt tgctgtttcc
     8941 ggtaacacgg tactctgtgt ttcatttgct gccagtgtcc cttgataaac ataggcattg
     9001 tctactagca cacctaacca tacacgattg acagcagtaa attcaagggt tacaggcttg
     9061 gtagcgtctg tgatatttac tgcgatggca ctgccagaat cttctcctgt agctattgcc
     9121 attttctttt cttcacttga ttttgttgtt gattctactg tagaagactc tttggtctgc
     9181 gtgctttgct ctgaggaaga ggactcctcg actgatcctt ctactttaag cgaagaagct
     9241 gtgtctccga ttattggatc agcttgacga tcttgccaag tcatatagcc cacgacaacg
     9301 acaatcgcca aagcaaccaa tcctaataaa atgacaggaa gacttgtcca aaagcgactt
     9361 ggatgcgatt cttcctgatg cattgctttt ctagaagtac ttactgtttc aggctgcgga
     9421 cgttcaggga ccgatcctgt caatttgctt ttcccatcaa aaacatcgac taaatggtct
     9481 ccatcttctc ctacagcttc agcgtattgc cgaataaatg cccgcacgta aaatttacca
     9541 ggcaattggt caaaatcatc attgtcgatt gcttccaaat aacgtttttg aatttttgta
     9601 atctgctgta attcatctaa agatattttt ttattcaggc gtgcttgccg taatttttct
     9661 ccgatggttt caaaagccac tcgttcatct tctccagtct gtttgattct ccgaagcatc
     9721 tgctgttgca acgaaagacg ttatgtttgg gtgctgtgac aaaagtcatg tcacagctcc
     9781 ttttttccga ataaacattg ttctcctgcc aactcctcgg tcacaagtca caatatccaa
     9841 taaatataag aagctgtttt ttgaacttgt tccttgtgac tcggagttaa acggcaggct
     9901 cacgacctct ttcttaaacg gtgttcaatc agctgttcct cggtattagc tcaaaaaccg
     9961 gcaaaatata aggagatatt tttccgtatg tttctctaat actcaggtca aaacgctgat
    10021 ttcacaacct cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagtccaacc
    10081 aagttaaaaa cattaagaag ctgtttttac ttggctgttc ttattacttg atgcagggca
    10141 gctttttcac aacctcttgg tacacggtgt tccagaagta gcttcttcgg caataagccg
    10201 aaatatcaca aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga
    10261 ccacttctgt cacaacctct ttgattactt cgtttgattt tattttaata taagttgatt
    10321 aaaagagagc atgatttgtt ttattttctc actaattttt ccaacacaac taagaatacc
    10381 ataaaagctg attttggagg aggttcctac tacaagtcta aaacgaaatt taagactttt
    10441 cctcataaaa tactggttta ttttggtctc atgtaaaact cgctgaaggc ctcttcactt
    10501 ataaatgctt ccccagctgc cagtacgtct ttcatttgga tcgaatcgat gatttcagga
    10561 agatcaaata aagttcgatc gccaaataaa ctttgcgtaa attgattggc aatatattcg
    10621 atcgaattta atgattggaa atattgtcca agcattttct tcttcagcaa atcgagattt
    10681 gtttctgaca cttccggatc atcagcgaat ccaagaatga tttctttgac tttttctgct
    10741 gctttctctg gttcgtccgt atctccgctg aagtccgcaa agtgaaattc acgatccaag
    10801 ctgaattcaa aaccaaagct gtcatcgata attccttgat tgtacatcgc tagatagttt
    10861 cgagaagtat tgcctaaaat catttggaac aacagattga ttgctgtttt atagcgtaac
    10921 agttctgttc cttcttgcgg caatgtgtct aatcctttaa taccaagtac aaatttatct
    10981 cgtgtaatgg ctgcttccaa tgcactttgt ttgatgattt cttttgtgtt ttcagggaaa
    11041 taacgtacaa tttcttgctt tggagggaaa ttttttgctt cttggttttc ccgaataagt
    11101 ttcatcaatt tctcaggctc catcttacca actacaaata aaaccatatt gcttggctga
    11161 taaaacgtcc gatagcacgt atacaagtct tgagctgtga ttttgtcgat actttcaacc
    11221 gtgcccgcaa tatcgatatg caatgggtgt gtaggataca agttatttag tatgccaaaa
    11281 aacatccgcc agttagggtc gtcttcatac atttggattt cctgaccgat gattcctttt
    11341 tccttgttta cagtttcttc tgtgaagtaa ggtgcttgta caaaatcgat cagagtcgtc
    11401 agattctttt ctacctgatc cgtcgtagaa aacagatagc tggttttcgt aaaacttgtg
    11461 aaagcattgg cggaagctcc ttgtttccca aataattgaa agacatcacc atcttctttt
    11521 tcaaacaact tatgttctaa aaaatgggca attccatctg gaactttcac cttttctttt
    11581 tcaccatagg gaataaattc gttatcgatc gaaccataat tcgtactgaa caaaccatat
    11641 gtcttgtgat agtcattttt aggcaacaaa tacacggtca aaccgtttgg cagtacttca
    11701 tgataaagtg tttcattgat ttgctcatat tctgttttat tcatttgcat ttcctcctgc
    11761 taaaaagaag attgcctgca attcgatttg ctcagctact tgttgtatct ctttgatcgt
    11821 tacgctctcc atacgtttca accattcttc atcagataat tttgtttccg gcagccagct
    11881 gtccaaaaaa gaagcttcga ttgccgcttg cggagagtct agagaaagca gatattggtt
    11941 tctcagcatt gcttttgtct gagacagttc caactctgtt atttcaccat ttcgcaaact
    12001 ttctaattgc tcgtgaatca gatgaagtac ttgatttcgg ttcttttcat cgataccggt
    12061 ttggacactc atgaatcctc ggaacgtatc cacgctacta gaggcataat aagctagact
    12121 ttctttctct ctgacattca tgaaaagttt agagtgagga aaaccgccaa ataaaccgtt
    12181 aaagaccatt agcgcaaaac gttctggttc atcgtaatac acatttgtct gataagctag
    12241 gttcagcttt gcttgaacga ttggctcacg tacttgtcgt tccttcacga tattcacttg
    12301 aggttgggta taaaacagat ctggataaac tgtttcccgc gactcaaatg gtaattgccc
    12361 aactgcttca gctgctttgt ccggatcaac atcgccgaca acaaaaatgt cgatttgatc
    12421 atttgccatc atttcttgat agtaggacgc tagcgaacta gcagtgatct tgtccaaatc
    12481 ttcgactacg cccaaccctg ggatcttttg ttccgggctg ttttcaaaat aaagttcttg
    12541 caaggcatag gaagcaaaag tttgtttatc ttccttcaaa ctttccaaat atagacgcag
    12601 attgttcttt tccaagtcaa acgtcaactg atcaaactga tgatttttaa tatttggata
    12661 aaataaaacc tcttttaaaa aatctaccgc ttgagaaaaa aggtttggat cattgacata
    12721 ttcaccattc acaaaactga tccctgcgtt tagccaatgt aaatttcctt tttttctaac
    12781 tgataatcca aagcttgcgc cataaagctc tgcaagttgc gcacttaact ttgtctgatc
    12841 cgaataattc aaactatttg tttccaacat gctgctgaga agtgaacgtt tcgtgatgac
    12901 ttcttgttgc agacgcgcag taaaacggat aaaaatccgg atcgttttgt attgttttgt
    12961 tggtaagaca tgaagattca cgccttctgc caattgatac accatattta tatgctcctt
    13021 ttcacagttg attgcccgct tactctttta atgggtcaac ctgccttttt tacttattcc
    13081 tctgcttgaa atttataagt tcaggcaatt cgccgttttt tctaaatgat gttacacttt
    13141 atagtatagc atagatgttt tttatcaaaa gaaacagacg gctaaaagcg tatattgttt
    13201 ttttattttt tatttttcga tatactaaag acaaataaaa gaagggaggg tcattcatga
    13261 tcaaagaatt caaagagttc atcatgcgtg gagatgtctt ggatcttgct gtcggcgttg
    13321 ttattggtag tgcgtttaca agcatcgtaa accaagtggt gcaaggcttg atcacaccac
    13381 taataggttg gtttgtcgca atgatcacag gtacttctga tttagaagga gcactttcaa
    13441 ttcttgactg gtcgccgaca aaaggcgtca catttgcgtt tggtaacgtt attagtgcta
    13501 tcattacatt tctgatcact ggttttgtct tattcttagt tgtcaaagcg gcaaaccgtg
    13561 cgaagaattt acgctccaaa gaagagcaag tggaagtgga agaaactcct actgcagaag
    13621 attatcttag tgacatacgc gatcttcttg ccaaacaagt ggcagaagac aatacacatc
    13681 cagtagagca aacaaatctt tccgatactt ctaaataata aagaatctaa aaaaaaagaa
    13741 agccgatttt cagtgtcggc tttctttttt tagattctta acttgctact tgttcctttt
    13801 tcatgtcgat atgtggaata tcatcttcca aatacacatc tgagattgcc ttgaatccaa
    13861 acgactcata aaaagcttgt aggtattctt gtgcgccgat cacgattggt ttttctggaa
    13921 aatttttctg gatcatgtta atcgtttctt gaacaatctt tcttcctaaa ccatctttac
    13981 ggttgtcctg atgaacaagg acacgtccaa aatggatact ctccgcttct tcatatactc
    14041 gcgtgtaggc aaggatcttc ccatcagtat cttgaaaaaa cgtatgccaa gcttctccat
    14101 caatgtcatc aacttcttga taaggacact gttgttccac aacaaaaact gcgattcgtt
    14161 ctttgacgat cgcgtaaaac tctgctgttg acaattcttc aaatttcttt acctgatatt
    14221 ccactaagca aacgccccct gcttttttat tcttagcata ccgattcgca cacaaaaaag
    14281 caaacgaacc ttacgaatct caggctcgtt tgctttttaa actgcttcag ttaaaaattc
    14341 gatctcaact gtccgagtaa gtacatctga atagctgtaa gatactcgtt caaaagaatt
    14401 ttcttcagga tccaaatcaa caacgaatac tgaaggatag gtttctgtta aaacgccgcg
    14461 gcgttctgtt tgacgctttc ttcctgtctg agcaaccaac atgattgggc tgccgatacg
    14521 accttctaaa tctttcttga ttgaagctaa agttgttggc atacagttca cctctactat
    14581 caactatacc acatattttt aaaattttca agtttaccat aaaaaaaaaa gaaatgcaat
    14641 aaaaataaat aagaagctca ttaagatttt tctaaattat tcctcattat aaaatttcaa
    14701 gtgttttttt attttgagtt ttactctccc gtaagcgctc attacttgct tgtgatccat
    14761 tgctaaacat tcagccatgt ctacaaaatc aacgccttgc atataataat gaaaaatttt
    14821 agcttctatt tttgaaaact ggatttcgca attttcgatt gcttctttta ataaaagatg
    14881 ctgaattgct aaaggttctt gcaatgtgat ctctgctaaa aagtctggtc cttctgtctc
    14941 tattttttgg tcgattgaga cttcatcttt ttgatcacga cgcttcagcg cgttctgctt
    15001 cctcaacaga gaatagattt gattgcttat aacgctttta taataacttc caaagctaac
    15061 acctttgctt ttttcgtaag ttctcaatgc ctgatataaa gcgatccttg cttcttgttc
    15121 ccaatcctca gaaacataat ttcttaaata ataggtgttt ttcaaacgta gtaaaagtgg
    15181 aaaatatttt tgatacaggg cagcaaatgc ttcttcgtca ccattacgtg cagcgcgcaa
    15241 taactcctca gacataaaat aattcctcca taaattcgtt tatggaggaa tcttatcatc
    15301 aatgattcat cgaactgatt agaacgatat gttatttatt ttttctcatt aaatcgtcca
    15361 atttttggga caattttttt aactgctctt cattccatgg agaattacgt cggaaatcaa
    15421 taaaatgcat atctttattt tcttgggcta ttgttttttc cgtttttttt atcgtcttat
    15481 ataattcata agcagaagtt cgtaatgctc cttgggaaaa gatcatccac tgttctgcta
    15541 aatcgcttgt cgccactgtc acttgtgtca aacggtcatt cagctgacca gcgattctct
    15601 cgatataact gtcagccgtt tcgtcttctt tcgtaaaaac aacatccaac tggtatttct
    15661 tataattttg ttggatgccc ggaactagtt gggcatcgaa gacaacgatg acttccattc
    15721 cttcatattt cgcataattt gataaacgat gtaaaagagc ttctcttgca tccgccattt
    15781 tgtcctgctt tttaagctgg acaagttccg gccacgaacc aatcatgttg tagccgtcta
    15841 caatcaacag ctgttttttc atggtctact ccccctgacg ttgggaaaaa gcctgataca
    15901 taagcaaacc agcagcaaca ctggcattta agctctggac gtgcccggtc atcgggatcg
    15961 tcagcaattc atccatttct tttttcaatc ctggactcat tcctttgcct tcattaccaa
    16021 tgactaaagc aattgatcct ttggcattcc atttttggaa gtttgtgccg gtcatatctg
    16081 tgccaaatac ccaaaatcct gtttctttga gctgttgcac agcttgtacc aaattcgtca
    16141 cgcgtgcaac agggacatgt tcgactgccc cagtggaagt ctttgttacg ataggtgtga
    16201 ttccaactgc tcggtgtttt ggtatgatca caccatcgac gccacttgcg tctgctgtcc
    16261 gtaaaatcga cccaaaattg tgtggatctt cgatgctatc caaaatcaaa aagaaggggt
    16321 cttctttttt ctcttttgtt cgttcaatca gttggtcaag ggataaatat tcataaggtg
    16381 taatcgcaag aacgactcct tgatgcacgc cgtgatcgct caaagtttct aatttttgtt
    16441 taggaaccca tttgactggc acagcttgtt cacgtgcgat ttctttcagt tcttctactt
    16501 tttctcctcg tgaatcttct tgaagaaaga gtttattccc tcgcccttgt tgtagcgctt
    16561 ctactgcggc atggtgccca aagacgaaat tatcttctaa tgcttcttca ttttgatcag
    16621 aaactgactg atttcgtcca gtttgctttt tatttgaata tctattttgt tttttgtctt
    16681 caaatccacg tttcgattga tgtttgtttg ttctttcttt acccattttt ttcaccaact
    16741 ttctgaatac accagcggat caattcttcc atccgctcgg tttgttttgt caaatgcaga
    16801 tagcccatga gcgcttcgaa ccctgttgcg acccgatacg tcgtaacatc agcatttttt
    16861 gcgctcgtat ggcttttagc atttcttcct ctgcgataat aaagttcttc ttcttctgtc
    16921 aaaagatgtt cttccagcat ttgctgaatc aaaaaggctt gtgcattcgc agaaacataa
    16981 ttcgttgccg cacgatgcag cttattgggt tttgtttctc cttgatcaat caggtggtca
    17041 cgaacataga tttcgtaaat cgcatctcca acataagcca aggctaaacc gttcaattgt
    17101 gtgtaatctc tcatgcttct cttctccatc gggttccttg cggtgtatct tctaataaga
    17161 tccctttttc tttcaacaaa tcacgaattt cgtcgcttcg gacaaaatca cgattgcttc
    17221 ttgcttgatt tcgttcttcg atcaattgtt caatctcttc atctaataac tcttctgctg
    17281 taaataaaat accgaagatc gaaagccatt gcgagaataa agcttctcct tgtgccaaga
    17341 cagcggcaga aactttcggc tggtctagat aagtgttcag ccacttagca agctcataga
    17401 ccactgtgat cccatttgct gcattaaaat catcgtccat ttcttcgatg aatcgctgtt
    17461 ctaaattttc taacaaagcg attttatccg catcatctgc caaattctct gaggcagttt
    17521 cttggcggaa tttcaaattg tcaaacgtat tcttcagtcg ttgtaaattg gtggctgctt
    17581 ctttcatcgt cgtttcactg tagcggatcg gacgacggta ttgtgtggtc gccatgaaga
    17641 aacgcaagac ttgtggatca acgtttttga tcatttcatg tacagtgatg aaattcccta
    17701 gagatttact cattttttca tcgtcttcgc caatcgttac atagccattg tgcatccagt
    17761 aattagcgaa ttttttccct gttttcgctt cactttgtgc gatttcattt tcatgatgag
    17821 gaaattctaa atcttgcccg ccaccatgga tatcgatcgt ttctcctaaa tgttttgttg
    17881 ccataacgga acattcaata tgccaaccgg gacgtccttt gccccatggt gaatcccaag
    17941 aaatctcgcc aggttttgct tgtttccata aagcgaaatc taatgggtct tctttttgct
    18001 gctgttcttc ccctgtacgc tgacttgctc cgacttccag ttcatcgatg gattggtcac
    18061 ttaatttccc ataattttca aatttacggg tacggtaata cacatcacct gaagattcat
    18121 aagcatagcc tttttcaacc aacacttgaa taaatgctaa aatatctggc atatgatcca
    18181 tgactcttgg atgcagtgta gcaggttcga cattcaacgc tttcgtatct tcttcaaatg
    18241 ctcggatgaa acgatcagca acttctggtg ctgtaatacc taattcgttt gctgtacgga
    18301 tgattttatc gtctacatcc gtgaaattcg atacgaaatc gactttatag cctcgatatt
    18361 caaaatagcg tcgaattgta tcgaaagcaa tggtgctacg agcatttccg atatggatat
    18421 aattatacac agtcggtccg cagacataca taccgacatg tcccttttgt atagggatga
    18481 attcttcttt ttttctggtc aatgtattgt agatttttat cattctgatc tcctcacttt
    18541 ttctcctttg attcgaacga cttttgctgg tactcctacc gctgtagcgt cgtctggaac
    18601 atctttcaac acaacggcac ctgcaccgat tttggcgttt tttccgatcg ttaccggtcc
    18661 taatatctgc gcattcgcag agagcatggc tccttgttta acagttgggt gtcttttacc
    18721 cgtttctttt cctgtgccgc ctaatgttac gccatggaat aaaatcacat cgtcctcgat
    18781 ttctgcggtt tcgccaatga cgattcccat tccatggtcg atgaacacgc cagttccaat
    18841 cgttgctcct ggatggatct caacaccagt taggaaacgc cagaattgag cattgatctt
    18901 cgccaataaa aagagccggt gctgatataa aaaatgagag aaacgatgcc aaaacaaggc
    18961 atgaaaacca gggtatgtca aaacgatctc agctgtcgaa cgtgcagccg gatcgtttct
    19021 ctttgctgca tcaatcgctc gggtaaacca actcatgact ttccactcct ttcgtaagaa
    19081 ttttatgtag gaaatcatct tcttataaag aaaagaagcg ccttttaaat aagtttcctt
    19141 atcaaaagac gctttccgcg tggttccact tttcttcata atcaatgatt atctctgaca
    19201 gctaacgcct gttatacgtt cttggctact ttgtttccta taaaacagtt tttcctgttt
    19261 tctgtgtttc gccaagacca ctcccagagg catttcggca accattcatt gataccttgc
    19321 accacccggt atctctcttg taatgatcag ctgcttactt ttttctgttc atcgtgttta
    19381 gttcagtact tgatttaaat gtgctaatga tttttcacga ccaagcaatt cgatcgtttc
    19441 aggcaattct ggaccatgca tttgtcctga tacagctaca cggattggca tgaaaagatt
    19501 ttttcctttt acgccagttt ctttttgaac agctttgatg gcagctttga cagttggagc
    19561 atctactact tccatttgtt ctagctgtgc tttgaatgcg tggagaacag taggaactgt
    19621 ttcaccagca agtacttctt ttgctgcttc attcaataca ggatgttcat tgaaaaatag
    19681 cgtagacaga tcaacgattt ctgcagcata gctcatttga ggttgataca ggctaacgat
    19741 cttcttcacc cattcgattg tcttttcatc tgggttttct gctacacgtc catcagcgat
    19801 tagataaggc agacacatgt cagtaaatgc atctagatcc attgctttga tgtaatggtt
    19861 gtttacccat tgcagttttt tcgcatcgaa agctgcgggt gatttactta ggcgttttgg
    19921 atcgaacatt ttgatcaatt cttggtgttc gaagatttcg tcttcgccca caggtgacca
    19981 gcccaataat gcaataaagt tgaacattgc atctggtaga tagcctaatt cgcgatattg
    20041 ttcgataaat tgcaagattg attcgtcacg tttgcttagt tttttacctg tttcactgtt
    20101 gatgatcaat gtcatatgtc cgaaggttgg tggtgtccag ccaaatgctt catagatcat
    20161 cagttgtttt ggtgtattgg caatatggtc atctccacgt aaaacgtgag tgattttcat
    20221 caaatgatca tcgaccgcaa cagcaaaatt ataagttggc ataccatcgc gtttttggat
    20281 gacaaagtcg ccaccgatat tatcggattc gaacgaaata gcgcctttca ccatatctgt
    20341 aaatgcgtat tctgtattgc gaggcacacg gaaacgtaca acagattcta gaccttgtgc
    20401 ttctttttct gcttgttctt caggtgtcaa gttcgcacat gttcctgcgt aatgaggcat
    20461 ttctcctctt gcgcgttgtg cttcccgttc tgcttctaat tcttcttctg tgcaatagca
    20521 tttgtatgct cgattgctag caagcagctg atcaatcagc ggttgataga tttctttacg
    20581 ttcagattga cggtaagggc cgtattctcc aggattttca ggagattcat cccaattcat
    20641 tcccaaccaa gcaaggtttt ccaactggct tttttcgcca tcttcgatat tacgtttttg
    20701 gtcagtatcc tcaatacgaa taataaagtc tccatcattg tggcgggcaa acaaataatt
    20761 gaataatgca gttcttgcat ttccaatatg caggtgccca gttggacttg gtgcatagcg
    20821 tacgcgtact ttcgtcattt tcttcctctt ctttcaaact agactttgac taacagcaaa
    20881 aattccgtca gttacggctt ctcaaatatc atttatttaa agccactcag cacaaatttt
    20941 accttttcta aggtaaatag taaagccaac tggttgaatt tccttaccag ttggctgaat
    21001 ttctttttta ttttgattgt ttagtctttt tctctgtctc ttttttattg tcgattcctc
    21061 gtcctgcatg tgcaggttta gcaaagatca tgcgtccagc agctgtttga agggcacttg
    21121 ttacaacgac ttcgatacgt tcattcatgt aatgctgtcc atcttctacg acaaccatcg
    21181 tgccgtcatc aaggtaggct actccttgtt ggcgttctgt cccggctttt actactagaa
    21241 cattcatcgt ttcaccaggg atgacgactg gttttacggc atttgccaac gcattgatat
    21301 taagaacagg tacattttgg aattcagaga ctttattcaa gttatagtca ttggttacaa
    21361 tgacaccatc taaaagctta gccaatttga tcaatttgct gtctacttca ctgatatctt
    21421 caaagtctcc atcgtacatt tcgacagata taccctcttc tttttgcaag gcatttagga
    21481 tatctaatcc gcgacgacca cgcacacgtt tcaaactatc ccctgagtcc gcgatgtatt
    21541 gtagttcgta aagaacaaaa ttagggatca tcaaggttcc ttctaagaat ccagtctttg
    21601 taatatcata aatccgaccg tcaatgatca cgcttgtatc caagatttta tatttatgga
    21661 aattttctcc tgattttcgt tctaacagtt gactctcaac ttgactagct gtttcttgct
    21721 cgattttttt gcttcgtgaa ccaaaaactt tcttccattc ttcgattcgt gtcgttccca
    21781 ttctcaatcc cagatagcct aagatgatca tcagtaaaat cggcaataca ctattgacga
    21841 atggaatttc catattgtaa agaggggtcg agatcacaac acctaaagtc aatccgatga
    21901 tcgcaccgat cgcaccgaag agaagataag tcaagctgac ctcacttagt ttttgttcta
    21961 cttgtttgat aatagctgag atatgtttgg ccaacagcaa agataataaa aagaaaataa
    22021 gtgcgcctaa caggctgttg gtaaatgtat tattcagcca tccgtttgac tgttgaccga
    22081 ttgcctgcca agctgagggt aaaaatgaaa tacccaagct tgcgccgaca atgatcatca
    22141 gcaaagtaat gacacgtttt tgcatcgttt ttcctccgtt tcgacaatca tttattagtt
    22201 agcggaacac acgcttcaac gtttcagcca aagttgctac tccaactatc tcgattcctt
    22261 ttggcgcttc ccagttacct aagttgtttt taggtaggta tacttttgtg aagccgagtt
    22321 tttggacttc tttgactcgt tgttcaatat gactgacacg tctgatttct ccagtcagtc
    22381 caatttcacc gatgaaacat tccgttggct gtgttccttt ttctttatag cttgaagcaa
    22441 tactgacagc gatagctaaa tcaatcgctg gttcatttaa ttttacccca cctgcagctt
    22501 ttaagtaagc atcttgattc tgaagaagca acccagctct tttttccagc actgccatga
    22561 tcagagatac ccgattgaaa tcaagacctg ttgttgttcg tttggcattt ccgaacatag
    22621 taggtgtgac taatgcttgg atctctacca aaattggacg tgtcccttcc attgccacaa
    22681 cgatagcaga gccagttgca ccgtctaggc gttcttctaa gaaaatctgt gaaggattag
    22741 cgacttcttc caatccatgc tcatgcattt caaaaatccc aatctcattc gttgagccaa
    22801 atcggttttt tactgctcgc aaaatccgaa aactatgatg ttgttcacct tcaaaatata
    22861 acaccgtatc caccatatgt tctaacattc ttgggccggc gatcgagcct tctttggtaa
    22921 catgaccgac gataaaaatg gctatcccat ttgttttagc ggttttgagt agttctgcag
    22981 tggtttctct tacttggctg acacttcctg ccacacttgt gatgtctggc tgcgtcatcg
    23041 tttggatgga atcaatgata acataatcag gggagatatg ttcgatggca cgactgattt
    23101 cgttcatatc tgtttcagcg tatagataaa attctgtgtt gatacttccg aggcgttccg
    23161 cgcgtaattt tatctgctct gcgctttctt cgccggatac atataagact tttccgccta
    23221 tggctgctaa ttgctgagaa acttgtaaca gcaaagtgga tttcccgata cctggatcgc
    23281 caccaatcaa gaccatagac cctggcacca cacctccgcc taacacacgg ttcagttctt
    23341 ctaattttgt tttgatccga ggttcttttt tagggacgac gtctgcgatt tttgttggtt
    23401 ttgctttttc tcctgtcaaa cttgtccgag ttctgcggtc cgttgtatct tgttcgattt
    23461 cttcgaccat cgtattccat tttccgcaat ttgggcagcg tcccaagaac ttaggcgaca
    23521 catacccgca attttggcaa acaaattgta cttttgattt ttttgccatt gtaccgcctc
    23581 cttttatcct aatcatgaga cattaaatca agcttaagta tctttaacgt tttatttact
    23641 ggaagaacca aatccgcctt gacgttgagc agtcgtttga tcttgatctg cttttaaaaa
    23701 cggcaaaaag atcccttggc cgatacgctc gccttttttg atcactacat ccttgaaacc
    23761 aaagttggtg aattggaaca tgatatggcc ttcatttgcc tcattattgt aataatcact
    23821 gtcaatcaca cctactccgt ttgtcaacac taaaaaccgc ttcaacggat tgcttgaacg
    23881 gttagcaagc tgcaaaaatt catcttctcc catgtaagct ttgattccag taggcacgag
    23941 ggttggtttc aattgttttt cattttctga tacatctttt ttcaatccat ctgcgactgt
    24001 ttccaacccc tttttccagc ctgctttcca gatacttgga atcacgacct cttctgctgc
    24061 ttcaaaatca tagccagcag catgatgtgt agcgcgctgc ggtagattga tattttgatc
    24121 tttatattta gaaataactt caaatcctct ttttctcacc ggttttcctc cctaaataaa
    24181 ctactcctat tctaacatat ccgaggctgt aaaagacttt ctttacaaat tcttcaagaa
    24241 attctgctat cttaaagtaa accattaatt cgttcttttt ccaaacgact gcaagttgta
    24301 caaaaaaact attttttaca tgactttact cgtcaaaaca agtagaatag aacgaaatta
    24361 atgagtggat atttgattga ggtgtcacat gcgtaaagaa attaaaaatg caaaacgaat
    24421 tattgtcaaa gtaggaacaa gtacattgat ttatcccaat ggaaatatca atcttgcggc
    24481 aatcgatcag ttagcttttg tcctttctga tcttagaaat cggggaaagg aagtgatctt
    24541 agtctcatca ggagctgtag gcgttggtat gcataaatta catttgtcag aacgtccgac
    24601 tttgattcct gaacagcaag ctgcggcggc tgttggtcaa gccgaactga tgaatatcta
    24661 taatcaaaga ttcagtgtat ataatcaaca gacggcacag atcctcgtga caagagacgt
    24721 ggtagattat cctgaaagtc gtaaaaatgt gaggaataca ttggaacagc ttttgattat
    24781 ggacacgatc cctatcgtga acgaaaacga tactgtagca gtggatgaat tagatcatct
    24841 gacaaaattt ggcgataatg atcagctgtc agccatcatg gcaaaacttg tcgcggcaga
    24901 ccttttgatc gtcctttctg atatcgacgg gttttattca gataatccga ctacaaatcc
    24961 cgaggctcag atgtatcgct atatcgataa aatcgatgaa aagattctgg cacaagcagg
    25021 tggtgctggt agcacatttg gaacaggcgg tatgcagagt aaattaaaag ccgcttctag
    25081 gatatttgaa gcaaacagcg caatgatttt agcaaatgga aaaaatccgg caattatttt
    25141 tgatattctt gcaggaaaag aagtaggtac tttatttaag gaggacttga aatgactgac
    25201 ttgatgcaat taggacaaca ggcaaaagat agcgcgcaac aactagcact tatgcctaca
    25261 aaccgcaaaa atgagctact tgttcaaatg gcacaaacca tcaaaaagaa tcaacaagcg
    25321 atcattgaag caaatcataa agacttagaa aaagcagtcg aaaacaatat cagtgaaacg
    25381 atgcaagacc gcttgcagct gacacctgag cgaatcaatg ccatggctac agagatcgaa
    25441 aaaattgctt cgttagacga tcctattgga aaagtagatg agatgtggac gaacaccgac
    25501 ggattgagaa tcggcaaaaa gcgcgtgcca ctaggcgtga tcggcatcat ttacgaatct
    25561 cgtccaaatg tcacgacaga tgcagctagt ctagcattca aatcagggaa tgccgttatc
    25621 cttcgcggtg gcaaagaagc attcttctcg aatcaattat tagtccaatt attgcagcaa
    25681 gtactggtca gtgctggcga atctccttat gccatccagt ttgtcgatga tacttctcat
    25741 gaaactgcag gacagttgat gcgattgaca gaatacttag atgtgctgat tcctcgaggc
    25801 ggagcaaaat taatccaacg agtcaaagaa caggcaacgg tacctatcat tgaaacaggt
    25861 acaggaaata atcacgtata tatcgataaa gaagcacagt tatctatggc agtgaacatc
    25921 atcgtcaatg caaaagcttc ccgtccatct gtctgtaacg cggcagaaac attgctgatc
    25981 cattctgaga tcgcaccttt tttcttgcca gctattgaaa aagaactggt agaacacggg
    26041 gtctctttac gtgcggatgc tcgtgcgtta gaatatctag agacggctgc tttagcagaa
    26101 gacacagatt gggatactga atatctagat tatatccttg ctgtcaaagt ggtcgattct
    26161 ttggatgaag caattgcaca tatcaatcgg cataatacta aacattcaga aacgatcgtg
    26221 acagatagtt atgcggctag tcaacgtttc ttaaatgaag tggacgcggc ggctgtctat
    26281 gtcaatgcct ccactcgttt tacagacgga tcggtatttg gttttggtgc agagattggt
    26341 atctctactc aaaagttgca tgcccgtggt ccaatggggt taaatgaatt aacttccaca
    26401 aaatatatca tttatggcga tggacaaatc cgcgaataat gtttcgtgtg aaacattatt
    26461 cccgcctttt gccttttttc tgtaaaggtt ctatccaact agaaaggtgt caatatgttt
    26521 gaaattttaa tgattttttt tatttacctt atttctctga atatcgctgc ttttctggga
    26581 gtcggtattt tatccctttt ctttcaattc aaaaaacgaa gcatagggag tcaacgggaa
    26641 aaatggtcgc agtattttga caaaatcggg caaaaagggc tggttacacg cttgcacatt
    26701 agttacatgg ttgcattatg cttattagcg acaatcaatt actattcttt tttcgatcat
    26761 tcaatcgctt atacgattac gctactgatt gctgggattt ttcacttatc ttataaatat
    26821 cagctgaata aaaaccactt gaatcggact ttccgctaaa aaatatccgt acgataagta
    26881 gagtgccttg aagatcggca aaaaactgcc ggacttttta gaggctgtga caaagatttt
    26941 gtcacagcct ctttttccat ataaacggcg ttcgcctacc agctgcctgg taaatctgtt
    27001 tcctcttcgt taagaagcga aaatccgtca aatgtttcgc catcataata acgaagcagc
    27061 tcatccatgg acaagttcat cgttttttct ttttcttctt ccgctgattc agccagtagc
    27121 tgttcccgat acacctgata cataccagct attttttgct gcatcgcttt tctagtttca
    27181 ccttccgcat tgattattgg caattctttg atatagccac gataaatcaa ccgatcatct
    27241 gaaaattcaa tgggtaattc aacgagtgtc aacccgactt cctgaaaata ttcttccagc
    27301 atctattatc cccccattct ttttcttaaa tgaattttag cttacctact gaaaattttc
    27361 cagtttcatt attttgagaa aaaaagggaa tgtgacaaaa atcgtgtcac atcccctttt
    27421 tccgaataaa tggtgttctt ctgccaactc ctcaatcata agtcacaata ttcaacaaac
    27481 atgagaaact gttttttgaa attgttcctt gtgattcaga gttaaacgac agtctcacaa
    27541 cctcttggta aacggcgttc aatcagctga ccctcggtat tagctcaaaa aacggtaaaa
    27601 tatgagaagc tatttttccg ttttgtttct ctaatactta ggataaaacg ctgattttac
    27661 aacctctgtt aaacggtgtt caatcagctg tccttcggta ttagctcaaa aaacggcaaa
    27721 atatgaggaa ctatttttcc gtttgtttct ctaatactca ggacaaaacg ctgattttac
    27781 aacctcctga taaacggtgt tcaaaagctt gcatctgcgg taataagccc aaccaaacga
    27841 aaaatatgag aagctatttt tgtttggctg ttcttatgac ttgatgcagg acagcttttt
    27901 cacaacctct gatttcttat tcttcttcat ttgtagcaaa ataattacgg tgataaatga
    27961 tacaacatac actgataaga acaatggcac ctgcaaaata aaacgcatag ctttcaccaa
    28021 tatgttcacc aatcacacct gcaataaatg gtgctaaagc tccaccgaaa aagcggacaa
    28081 aactatagct ggacgaagca acgtttcgtt gcagacctgg tatttccata gcaatcgtcg
    28141 tcaacagtgt attgataatt ccttggaaaa atcctgctaa cacaatgctg atcggtacaa
    28201 ggactggcat agaagttcca gctcctaata acagcaaaca aataatgaat aagacaaatg
    28261 ctgctaaaat tgtccgataa gtcgtaaact tccgctcaag agccggtgca acgaaaatcg
    28321 aagcaatagc cagaagcaca ccccagccaa aaaatacaaa accgacttct aattcagaat
    28381 agccaatcaa taaaaatggc gcatacgcaa gtaaagtaaa gaatccgaaa ttatataaca
    28441 atgcgattag tccaactgaa cgaagtttat gattgcttaa cgctttgaca ccttcccaaa
    28501 ggcctacttt tctctttggt ttctcagttt caggtaaaag taccgctagg gctataaaac
    28561 cgcaaaacat caaaaatgct accccaaaaa atggataacg ccatgaacca ctgcccaaga
    28621 ccccaccgac tagagggcca acagccattc cgcagccaag tgctgcttca tacatcatga
    28681 ttgctttttc tgtgttacca actaaaatac tgacgatcgt tgctaaagca gtagagacaa
    28741 ataaagcatt cccgacaccc cagccggcac gtaacccaat cagcattccg atattcccag
    28801 ataatccgcc aagaccagcg aatacaacga tgataaacaa tcctgtcatc aatgtttttt
    28861 tcaaaccgat ccgactagat aagaaacctg taaatagcat caccacacca gtgactaaca
    28921 tgtaactagt aaataacagg gtcgtttcag ctggcgtagc gtttaactgt gcagcaatcg
    28981 tttttaagat tgggtctact agacccactc ccataaaagc gatcatacag gtagctgcta
    29041 caactagcgc tgaacttttc ccctttgttt cttcatttgt ttgttcttgt atatctattt
    29101 gattctccaa tatttccatc cctttctctc tattagcatc atacatgatt tattttacat
    29161 gtcaatatgc acatgtaaaa cgtggaaact ttactgtgat ataataaaga aaataagacg
    29221 agaggtagga tttttatgaa tactttagcc tatatattat tatgcgtgct tttatccaaa
    29281 ccacgttctg ggtatgaatt gaagcagctg atcacaattt tttgggaagc ccatcacagt
    29341 cagatctata caacgcttgc caagttagac cagcagggtt atgtgaccgt tttggacaaa
    29401 gacgagcact ctcaaaaaaa gatctaccac ttgactgatg aaggacgtgc tcttgtagag
    29461 gaatggatca aagaggaaac tcccgctcct acgcaaaaag atgaatttct agcaaaaatc
    29521 tatgcttttt cgacgctcga taaaacaaca gctactggac tgctattcac tagagaacaa
    29581 cagctgaaca aaatcatgca aaaaaatcaa aaaaaattga atgacctctc gctagacaag
    29641 aaacaagaat ttggacgtta tgttgtgatc cagcgcagaa ttatgctttg tcagcaggaa
    29701 ctccaatggt gtcagcaagt aagagaacaa atgcagcagt tttttgagta agggataaac
    29761 agaacctttc tagaaatatc tattgaagag caaattatct tcaataaaag agcaatacag
    29821 ttggaaaaga aaaagcgatc cgaacgacag gccaaaacct atcgttcgga tcactatttt
    29881 tctagaactg cattcaagag actatgacaa aaagcatgcc aaggcctttt ttcaaataac
    29941 agtgttccat cattcattac tttgaattgc ttttagcaca cttcttgaaa gcgggccaac
    30001 gatgatcaac tgcaacggta aagcggcaat cacattcatt ttccaagcat tcatataagc
    30061 agagaataat tgatcaggta ttccttgttc catgatcaca ccgaataaag acatacatgt
    30121 caccattcct aaaaccatca agcatgaaat agttaagatc atttggacct tgctttctgg
    30181 atttactggt agtttaaagg caattttttt cgccagcaca ccgatgatga agacatccaa
    30241 aatgaaagcc accacgaatc caggcactag accaatcaac aaacttgata aatgaagatc
    30301 ttgatgcagc cataaattat acgcgctcat ccccaagacc atcaaaaaac acatcattgt
    30361 tgtaaaaatt attccttctt ttttatttgt aggcaatctg taccactcct ttaaatttgt
    30421 cacgaaaatc atcgtagcac atttaaaaat ttctcgtaag tacttttttt attttctgcc
    30481 cttttatttc ataaacttca tcagccgtac gctcgacaaa gtaagaatca tgagaagtaa
    30541 acaaaatcgt tcccggatat atttgcatta gcttctctaa agcttcgatc gttgccatat
    30601 caataaaatt agtcggttca tctaacagca acacattact tggcttagta aaaagcaaag
    30661 caatcgtcag acgggtcgct tctcccccac ttaatgtaca taacggtttc gtcaaagttt
    30721 cctgagcaaa ccctaagtga ttcagcaaac tacggacaag cgattctgaa aactctgttt
    30781 gtctcatgac aaaacgaatg atggattctt cagatgtcat ttgataaccc ttttgctgat
    30841 atacttgaaa gcttactttc ggagagagaa ggattccttc tccttgtttg cggatatgtt
    30901 ctaataaggt tgtcttacct gaaccatttt caccgacaat cgcgattttt ttacctaaag
    30961 aaaactgaaa atcaccattt accagtaatg ttctcccact tctttccaat tggacatttt
    31021 gtcccatgat gggatagcgg ctgtggagtt caagagattt tggtactgga aaacggattt
    31081 gtttcatctg ctgcggcttg gttgtttctc ctattttttg gagtcttctc tctaatgttt
    31141 tcgcattctt ttggatggct ttttgtacac tgtctttttg tttagaggaa gacaaccgat
    31201 ccagtctgat ttgttgcttt ttctttttac tggacacttt ttccaacttt tccgcttgtt
    31261 ttttcttttc ttggatcgat ttttccaatc gttttttctc tttcatgatc gactctgctt
    31321 ctcgtagctg agtctttttc tccaattcct tttgagtgag ataggcacta taattccccg
    31381 aaaactctcg gatttctccg tcttttactt cccagatttt cgatgctagc tcatctagaa
    31441 aatagcgatc atgactaaca aaaaccagcg taccataata ataacgaagc tcttcaatca
    31501 gtcgatcaat actttttcta tctagatggg ttgttggctc gtctagaagc atcccttgtt
    31561 cataagaaga aagaagacgt atcaactcga tctttcgttc ttcccctccg ctccatttcc
    31621 gctctgaagt ttcttccagt cttaactgga aatgactggc taattccaaa tttaggtcct
    31681 ttgcttcagc gagataggtt aattgaggca ggtagttgaa ggtgatctct gtttggattt
    31741 tcccatgatc gggaaaaagt tcaccggcta ttagtttgag caaagtcgat ttaccgacac
    31801 cattttctcc tatgatccct atacgatctt gctgatagac acgaagagaa tcaatagata
    31861 gttctagctg gttgccaaag ctgacttgta gatttgttat atttactgct aaattttcca
    31921 tatgttttac actcctattt catgtaaaac aacaattatc gatactaaaa aagagcagaa
    31981 aaatctgttt gcaaaaaaac tgcatcacat cttttcctac tctgaacgaa ataactatga
    32041 ctaaatcacc aaatctgtca tagctttcac ctattctact gtcgttcgaa aaaatggaca
    32101 tactccatcc atgaaattgt tgtttataca tgtcttttga atttatttag ttgttattca
    32161 aaagttcgaa acgtaatttc atcgatgcag tcatttttcc gacctccttt tttattaggt
    32221 taccacacac gattcaaaaa aagcaatagc tactgaatag taactgaaaa ccttttattc
    32281 gaacatagtg atcttttctg gtgctaaacg aacagactcg taaccttctt ctaccgcttt
    32341 tcctattgat aaaataataa ctggaacata acggtcttta tccaaaccaa atgcctctgc
    32401 caactgatct gcttcgaatc caccaattgg atttgtttca tagccatgat cacgcgcaac
    32461 taacatgaat tgcatagccg ctaagctaga atcgactttg actacatcgt tcatttcttc
    32521 tcttgaaaaa ctcttgtaat aaggaatgat cgcagctaat tgttgatctc tgacttcttt
    32581 tggcatcttc cctgattcgt aagcttggtt gtagatttct tcgccatatt cgtaacattg
    32641 catatcgcca aaaatcatga ccattgcaga agaagtatca ttttgtctcg tattgaaacg
    32701 gatcaaagga cgcaagattt cttttgcttc ctcgctttca gcgaccacaa agcgccatgg
    32761 tttcatattg acagaagaag gtgcagtcgt cgcttctttg atcatttcca gcatttcttc
    32821 gtgtgggatt ttgtatgttt catcatatac gcgaacggat cttctaccag tcataattgt
    32881 tgaaaaatca tttttttgtt ccattattat taacctcttt cgtttctgtt gttatattgc
    32941 aaagttgggt attcaattta tctaaaagtg ttagaaattg tttcatttct acatcgctga
    33001 aagccgggga tagcacggaa cctgtatctt tatggtccat ttcacaatgc ttgattttct
    33061 tctgggcttt ttctgttagc gctacataca cttcccggtt gttctgagga tttctctgac
    33121 gcgaaacata gccatttgct tccaatacct tcagatgacg agtaactgct ccttgatcaa
    33181 tctgcaactt ttcttgcaaa ctgctttgta gcaatggatt ttcttctttc agtctcatca
    33241 tgatctcgta tctagtaaga cttataccga tattttcttc aaagatctgg acgatttttt
    33301 gatcggctaa tttgatttga tataaaaggt gactaatctc ttctaaagac ataatttcct
    33361 cctttctaat aattgactcg tcaacgattg acttgtcaat aatagaataa aaaaagaaaa
    33421 aagtcaaaga atcagctgtg taataaaaaa aacgctctca atagaaaagt atagagaaaa
    33481 gaaatttttt gtagaatgaa cggaaaaaat gataaagaaa gcacaacgct aaaaccaaga
    33541 ttattgattc ttatcaaact atagaagata gctcttctgc agtagataat tttacaccgc
    33601 caaaaatttc cgaaatcgcc ttttgcaaga atcccgtcga atattagaaa ttggatcagt
    33661 atgcttaaac agtaataaaa aagttatgac aagatgttgt cacaaccttt tttgagtaaa
    33721 cggtgttcaa aaacttgcat ctgcggtaat aagttcaacc aaacaaaaaa catgaaaatc
    33781 tgtttttgtt tggctgttct tactacttga tgcagggcag ctttttcact acctcttttt
    33841 aataaccata aaatcctatt ccttcatatc tccagcccat cggtgttagc caatctcttt
    33901 ccttagcact caaggtatag tgatgggtac cagttttttc atttggatta aatagtcgat
    33961 aaattggctt ggaaccacca gaataaaaac ttagaccttc ataacgccaa ccgatttttg
    34021 ttaaagagtt gatctcagat tgtgccattg tgtaatgatg gtctccactg taaggattgt
    34081 ataaacggta gacaggttgt ccgctgtttg gtgcttgcca agcaatccct tcatattccc
    34141 agcctacagt tggcagataa tttttttctc cggcattcaa tgtatataaa tgttctcctg
    34201 tatgacggtt atacagacga tacatcggag aagttgaagt tggtgctgta tcatactgtg
    34261 tcagattata ggtttcgatt acattgttca atgtagaacc ataattaggc gcagtggcat
    34321 aacgacctgt tagccaagcc gtagcatctt tgtaagagtt cgtattgctt ttccatgcac
    34381 cggaatagta gtaaacgcct ggctgaaaag aagtcgtctt caataccgtt acgttatctt
    34441 gcaatgattc tttgtacgaa ggatattttc taaaagcggc attcaccgta atccattgcc
    34501 cattcacata ttccgaagta ggcatcgtaa cagattggcc attatagctt cctttaatcc
    34561 caaatagatt gtaattaggg gcagaagcca aagtactgtt cccccagcca ctttcaacga
    34621 tggcttgagc aatcatgact gatgcatata agtcattggc acttgccaaa ctttgtgcag
    34681 ttgaggcaat actatcaatg aacgcttgtt gagaattagc agatgagcga gcataaaaag
    34741 catagccagt atagttttcg aattcagaca aatctgcttg atgggtatgt gtcaatgttt
    34801 gcccatctgt caattggttc gtgatttctt ctgcattctc tggattgttc tgagcttctg
    34861 ctgatgtact gtcttcggtt tgtgtttgtt ctgttgtttc agttgttggt tcttcggttg
    34921 aaattgtatt ttctgagaca gtagctgact gcaaagtact ttctgtatcg ttttcaattc
    34981 ctgtttctga agaactcgat gtctgagttg cgacagtttc tgtcgattct tcagctagca
    35041 ctgttgtgtt ggcaactgct agatttccta aaagaataac gctaaataat aaattctttg
    35101 ctttcatctt ttttctcctt tgaattatga aacttttcct aaataacagt gtaactaaaa
    35161 aagtaaaagc aagtcaatgt ccatatttaa ataacatttg tttcttttta ataaatttgc
    35221 tttttctgca taaaaagacg taaatgaaga atattcttct ccattcacgc cttaaattga
    35281 tggtcgtttg aataactatt taacaagtaa ttctctctgg atattatccg ttatggatta
    35341 tacttcctgt gtcgtcggac ggataatcat gttgctgaca gacatccggt ctggcgtttc
    35401 tatcgcaaat gctacagcag aagctatatc ttcagatgtt aatccccatt ctttttgcgc
    35461 ttcatgaagt tcaagagcag cctttttgtc gctgatggtt tgatataatt cagtttgaac
    35521 agctcctgga gagatgatcg tagatttgat gttattttgc cgttgctctt gacgtaatcc
    35581 ttccatgatt gcacggacag caaattttgt tccgcaataa acggcggaat caggataaac
    35641 aacatgtccc gccacagaat cagttgaaat gatatgccca gatttttgtt tttccatgat
    35701 tggaagtacg gcggaaattc catttaacac acccatgatg ttgatatcaa gcatattttg
    35761 ccattcatca cggtgacctt caactaatgg agcggttggc atgattcctg cattattgta
    35821 caatacatcg attcgaccga acttttccat cgctaaatcg ataactgctt ggacttgtgc
    35881 aaaatctctt acatctgctg tttgaatata tagctcagct tctggtaaag attcttttat
    35941 tgcttttagg cgatcttctc ggcgtgcagc tataactagt ttagcccctt tttctgctaa
    36001 tagcttcgtt gttgcttcac cgattccgct tgaagcgccc atgatgacaa tcactttttc
    36061 tgataaatca ttcattctat tctcctcctg ttttgaaaaa tctcttgctt atactcacta
    36121 tctcttattt catcttactg ttcaaataat ccgttcgaca ggtttgacga ttttggcagg
    36181 gattcctgca acgatggtat tgtctgggac atctttcgta acagttgcat tcgctgcaac
    36241 aatggcattc tcgccaatcg ttacgcctgg cagaatagtt gctcctgctc cgatccaagc
    36301 atttttcttg atgtggattg gctttactga caagcctctt cgttcttttg gatcggttag
    36361 atggttcacc gtgatgaggt tgacacgagg accaatcaat actgaatctt ctaaacagat
    36421 gccacctaag tcaacgaaca tcacttgacg attgataaag acatttttcc ctatactaat
    36481 atgtttgccg aaatctgtat aaaaaggcag cagaacagtc accgtatcat ctactttgtc
    36541 actaatgata ttttctagcc gtttgcggat atctgctggt tcatggtacc cagtattcag
    36601 cgctgctaaa ggctcttcgt tttccgcttg gatctgatgg atcacttcaa agagcggatc
    36661 cgttactcga atctctttat tgacgatttg tttttcgatg aggcttgtca ttttgctccc
    36721 tccttttatc cttactttat aacggacggc ttatgatgtc taatacttat aaaacatact
    36781 taactattcc ttttaactat acttagtggt atgctttttt aaaaacagga gggaaaaaat
    36841 ggatattcgt atactgacgt attttttagc agtagctaga gaaaaaaata tcagccgagc
    36901 agcaaaatct ctgcatatca cgcaacctac tctctcaaaa caactgaaag atttagaaga
    36961 agaacttggt gtgacacttt tcactcgagg ctcccgagag atacagttga cggattctgg
    37021 tcgatatctt tattcaaaag gaaaagaaat cttgtcgttg gtagaaaaaa cggcgaacaa
    37081 tttaacaaat gacgcaatca tctctggaga aatctacatt ggcggaggcg aaactaaagc
    37141 aatctctttc atttctgata gccttcattc gttgatcaag gcgcatcctg atatccagtt
    37201 tcatttatat agcgggaatg cagatgatat catagacaaa ctggatagcg gattacttga
    37261 ttttggactc gtaatcgaac cgacaaacaa acaaaaatac cagtcatttc gactgccagc
    37321 ccaagatcgt tggggacttt tgatacatcg gaatcatcct ttagcaaagc aaacagtcat
    37381 ccatccgcag gaactgaaaa ctgttccttt gatcatttcc cggcaaacgt cagtggacag
    37441 tcaactagct gaatggctag gtttcagcat agatcagtta tcgatcgtcg gaacatataa
    37501 cttattgtac aatgcttctt tgatggtcga taacgggact tttggtgcat tagctctaga
    37561 tgggatcatc aatacttatg ggacggaaaa agtattcatc ccactcgatc cgccattaca
    37621 agcttcactg aatctcattt ggaaaaaaga ccagcctctt tctcctgctg ctcgtaaatt
    37681 tttagatgtc ctaacggaga aatacagtta agtagagttt ttttccttcg ctttcagctt
    37741 tttaacaacg aataagttaa gcaaaagatc caatgattaa ataacataag tttcttaatt
    37801 tgttccttcc ttttatttct gataattcca gtaaattact ataattataa aaaaagaatt
    37861 gacaaaacat aactgtctat ataaaattga ttacgttaac aataattcgt ttcatgtgac
    37921 tagatcatac taggcatgaa aaatctctga ggaacatggt ttccctagga ggtttttcgt
    37981 gtctattttt gtttggccgc aatcaactgg tctagcaaac gtgaaaacca aatcttcaaa
    38041 aagggtgcgc gcatgaaaat caaaaagatc tttaatcaaa atgcagtatt ggtagatgac
    38101 ggtggacagg aaaaagtagc tattggaaaa gggattggat ttgacagaaa aagaaatgat
    38161 ttgatttttg atcgtgatgt cgaacggata tttgtcatgg aaccagaagg tcaaatcaag
    38221 ttgcaaaatt tacttaatca gattgatgaa cgctttttat ttgcagcaga aacgatcatt
    38281 gatcatgcgg aaactgtttt gatggaaaag ttgaatgaac atgtattgat cgctttgacc
    38341 gaccacattg ctttttctgc ggagaacatt aataacggta tcgtcatccg caacaaactt
    38401 ttaagagaaa tcgaagtgct ttacagtgat gaattcagta ttgctcaatg ggcagtagag
    38461 tatctgacaa aagagcttgg gattccatat acttatgacg aagcaggata tatcgcaatc
    38521 catattcaca gcggacgaag cggtcggaac aataaccatc gcagtatccg tgaagtgacg
    38581 attgtatctg acatcatcca tttagtggaa tcagaattgg aaacagatat ccattcagaa
    38641 ccattttcac tgaattattc aagactggtg aatcatttac gactgcttct acaacgaacc
    38701 catgcgcaac aatatgctgt tttagacacg gaaatcgtag aaatggtcaa acgaaaatac
    38761 ccagaaagct ataagatttc taaaaaaatc cgtgtgttgc tgaccaaaga ataccagctc
    38821 gcgatcacga aagaagaact gggttacttg gccatccata tcgagcgttt aagaagcgca
    38881 atcgtacaaa caaatgttaa caatcatgag gaggaaaaaa attgaaagca tatctacaac
    38941 gaatgggacg ttcccttcaa cttccagttg cggtcttacc tgctgctgct ttactagtag
    39001 ggatcggaaa ctggtgggca tcttatagta acgacattgt cgctcatttt ttacaagctg
    39061 gcggtaacgc agttttaggt caattacctt tgctgtttgc cgttgggtta gcactaggga
    39121 tgtcagtgga caaatcaggt gctgctgcct tggcaggttt ggtcgcattt gaagttccag
    39181 taaatgtttt aaaaactgat tcagtagcta ctttattgaa tatcaaacca gaatcagttg
    39241 atccatcttt tgctcagatc agtaacgtat ttattggtat tattgccgga ttgattgcgg
    39301 cagctcttta taatcgtttc cacgaagcaa aattaccgat ggctttatct ttctttagcg
    39361 gcaagcgtct agtgccaatc ttggctgcct ttgtcatgtt gctgatcagt gcagtgctac
    39421 tgctcgtatg gcctccggtt tactctgctt tagtttcttt tgctaaattc attttgagtt
    39481 taggctgggg tggtgcaggt ctttatggct tcttcaaccg tttactgatc cctacaggat
    39541 tgcatcaagc aatgaactcc gtgttctggt ttgacgtagc aggaatcaat gatattggta
    39601 aatttttggc ttctgaagga acaaaaggcg taacaggaat gtatcaagct ggatttttcc
    39661 caatcatgat gttcgggctt ccagcaggag catatgctat ttacagaaat gcacgtccag
    39721 aaaacaaagc aaaaacagct tccttgatgt tagcagcagc ttttgcttca ttctttactg
    39781 gtgtaacaga accacttgaa ttctcattta tgttcgttgc ttggccatta tatgtcttgc
    39841 atgcaatttt cactgggatt tctttagcag tgagtgcatt cttccattgg acagcaggat
    39901 ttgcctttag tgccggattt gtcgattact tcctatcttt gaaaaatccg attgcgaatc
    39961 agccattgat gttgatcgtc caaggattag taacagcagt gatttactac tttggcttta
    40021 actttgctat caaaaaattc catctgatga ctcctggccg cgaggaagca gatctttcag
    40081 atgacgatac tgcaacgact aattcagaca ataaatatgc ggcacaagca agcaaaatct
    40141 acgctgcatt aggcggagcc gacaatgtaa cttcgatcga taactgtact acacgccttc
    40201 gtttgcaagt caaagataca ggcctgatcg atcaaaataa aatcaaagcc actggcgtac
    40261 ctggtatgaa gatcatcgac ggaaaaaatg cgcaagtcat cgtaggtaca gaagtacaat
    40321 tcgttgcaga tgagatggca aaactacatg gtggtgctgc aacacgacct gcacaaacaa
    40381 acaccgtggt taaaacagaa acatttgccg gtggaacgat cgaacaagat atttacgcta
    40441 tcgcaaatgg gaaactgatt ccaatcactg aagtttcaga tgatgtattc gcggaaaaaa
    40501 tgatgggtga cggctatgct gtattaccag aaaatggaga aatcttctct ccaatcgctg
    40561 gtacgatcac aaacattttc ccaacgaaac atgctgtcgg aatccaaaca gatgctggta
    40621 tcgaagtctt gcttcatatg ggcatcaaca ctgttgactt aaaaggcgaa ccattcactt
    40681 tatatgtaaa agaaggtcaa aaagtcgcaa gaggtcaatt gattgcttta gttgatcttg
    40741 ccgctattca atctgcaggc aaaaacacgg atatggtcgt tgtattcaca aatggcgaca
    40801 aagtccaaag cttagaaatc gaacccgcac gtgatgtaaa agcaaatgat aaaatcggct
    40861 ctgtttccaa caaagcctga gccatcgtct tccccagact tttttatcga taaacaaaca
    40921 ggagctgaga caaaaatctt gtctcagctc ctttttccga ataaaaacgg cgttctcctg
    40981 ccaactccgc ggtaataagt ccagccaaac gtaacatatg aagagctatt ttttatttga
    41041 ctcttcttat tacttgatgc agaacagctt ttttacaacc tctgataaac ggtgttcaaa
    41101 aaccagttta tcggtatgaa gtcaaattta tgcaaaaatg tggaaagcca ttttcacaaa
    41161 atttttcttc atactcaaaa ctaatcggct ttttcacaac ctctttatac attattcctg
    41221 atccaatatt tcttttaatg ctgcttcaag agaagttaat ggttttccca atactttttc
    41281 taaatcatca gaggaaatag ctaactgatc ctttttaata tcatgttgga tcgctaaaaa
    41341 catgtcagct gcttcttgag ggaatccggc ttcttttaga ttttcgacaa acgctgcatc
    41401 acttgcttca ataatttcta actttttacc tgtcgcacgc tctaatgcct tggctaattc
    41461 ttcatagtca gttagattgc cggataattc taagatttct ggagagtcac tttctactag
    41521 tacttttgct gcagcttctg catattcacg tttcaatgcc caacctgttt ttccatttcc
    41581 tgcagcataa acgaattgac cagtttttaa agcctgccca atgataggta attcattctc
    41641 tacataccaa ttattccgta aaaatgtatg ggaaatacct gaatccttaa tcattttttc
    41701 agtgaattgg tgatctggcg ctaatacact gatcgagtga tcggcatctg caaaacttgt
    41761 ataagcgata taagataccc cagctgcttg tgctgccttc actacattct tatgttcggc
    41821 ttgacgattt cctggaacgc cagagacaaa aagcaatcga tctattcctt tcaacgcttc
    41881 tttcattgag tctaaatcat cataatcacc aatacgtata ttaaatccag cttctttcag
    41941 tgcttctcct ttttcttcac tacgtactag tacatagatt tcattttgtg gaaccatctt
    42001 ttttaagaat tccagcgcat aaccaccaaa acctccagtt gctcctgtta ctaaataatg
    42061 catatttgcc actccctatt ctttttattt gttaagattt acagctgtac atttataaat
    42121 tacaccttta tcataaagag tgtggtaaga taagtcaagc aaaaaaaatg aaaggatgac
    42181 aactgatgaa gtattctctg caatttagtg atgcaatcca tgtacttgcc tatattgaaa
    42241 tgtttcaaga tacccgatca ctttctagtg agatgattgc cagtagtatc gaaacaaatg
    42301 cagcaaatgt acgtaaaatc atgagtcagc tcaaaaaatc cggtttgatc tttacaaaaa
    42361 ccggaaaacc gcagccttcc cttgccaaaa gtccagatca gatttcttta ctagatgtct
    42421 atcgcagcat cgaaggtaat acacagctta tccaagtcga tccaaaaaca aatccaaatt
    42481 gcgtcgttgg tgcgaatatc caagaagctt tagaagaaag ctacgctcgg ctgcagaaaa
    42541 aggcagaaga agaaatggct aagatcacac ttgatagtct gatacataaa atagccgttt
    42601 tggaaaaaga aaaaagacca gaaaacacag gcctcgtaga gagattttta taattctctc
    42661 tactgtgcct gcatttttct gatcggaatc gtctacataa attcttttat tctccggggg
    42721 acaaaagcct tgtcatgccc ctttttcctc tctaatgctc aggtcaaagc gctgaagtca
    42781 tgacgtcttg ataaacggtg ttcaagtagc tgaccctcgg cattagttct gaaaaatgca
    42841 aaatatggaa agctatttcc catttttcct ctctaatgct caggtcaaag cgctactttc
    42901 acgacctcta tattatcatg gtttattgac taccggtatc cagatttctc ccatatattt
    42961 cccgttggta actcccattt cgactacagc atttggaccc ccaacatagg cgtaatcttc
    43021 tacttctctt aaaacatgcc caaaggccgt attcgtcaaa tcatttctta attcttcagg
    43081 cgattctgct aaagcgatta caacaagata cgttcctttt gggaatggga tgattcgggt
    43141 agtgtttgga acatcctgac tcgtttgaac accgacatag tacatcattt tcccgttata
    43201 gggttcgttg actgtaaaga aataatgatt ttcagcagct tgtttcaatg catctattgt
    43261 tccattaccg ataacttttt gctcaaatgc ttgtttctct gcagccaaac cgacaaaatc
    43321 cgtattgcta ctcttcaatt ccacaccaat acccgtcacc gtaaaatctg gtttttcttc
    43381 gatcctatac tttgccattt ctcccaccct ttctattata ttaagttcat cttacataaa
    43441 attaaaggga aatgacacga ttacgctctt ttttctaggg atatttttat tttttaacta
    43501 tttttttgct ttcactgcaa tccaaatctc actgtaaaca ttactgaaat cctctcccca
    43561 ttttgtaaaa gagatctcgg gagcttcgac taactcataa tctgaagaag gaagccattc
    43621 tgcatagatt ttcccccaag tatcttgtaa agtttgggga aatggccctt tattaggaaa
    43681 aatggcccaa gtgtgttctt ctatagcaag catttctaaa tcagcgtacg gattttcttt
    43741 tgtcgtagca aagccaatca tgtgcatcaa actgcccttt tcttccaagc gggctccatc
    43801 aaatccataa gaaacgttga ttacttgatt tggatataaa tctcctaact tatgcatctc
    43861 ttccctttga gtctccgtga tcgtctgcgc taacttttgt atttcttgat tgactccttc
    43921 aaatttgaca ggaacttttt tcgccacacc tgccacataa aactgttctt tcttttcgat
    43981 acgaaattcc atcgattttc ctcctctgac atcaataaaa aatgtcaagc gtggaaaaga
    44041 tttttgtatg ccgtatctgc tgatttctga aggcagatat cctgaccaat cacgaaaagc
    44101 tcttgaaaaa ccttcgactg tttcatatcc atatctaaac gccgtttctg tcactcttga
    44161 tccatctagg agatcgcgat tagccaaagc caatctacgg tttttgatat agtccattag
    44221 cgacatccct gcaataaacg taaagatgcg ttttaattga tattcagaga ttcctgtatt
    44281 ttcggctatt attttatagg aaagtccttc tgtcaaatgt tcttcgatgt aatcgatcaa
    44341 ttggttcaga tctttaagca tatttatctt cctccttgct tgccttttta tcatattatc
    44401 aaagaaagtc aattacttga tgaatcagga accaatttag tcagatgtag aaaatgttgt
    44461 atggatctaa agaaaattct caagcagcgt ttcgtaacgg ataattggta ttatctgcct
    44521 atctatgttt tacctttaga aaaataaatt ttggggaaga cacattctaa gtaaacaaag
    44581 cggtaaaaat atcttttatt cccttagtta atccgcttac tacctactgt acaaagcaaa
    44641 ttgttgttca aactgctgat cagaacgctg cttggatttt tctttgcgat acatcatacc
    44701 tcttttatca attggaacaa ttatttattt ctaacaaaat cacttcattt ttgtcagata
    44761 aataaa
//
LOCUS       DARDHA010000009        43782 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000009 DARDHA010000000
VERSION     DARDHA010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.009"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(137..481)
                     /locus_tag="SDA10_000395"
     CDS             complement(137..481)
                     /locus_tag="SDA10_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEG4239327.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(474..908)
                     /locus_tag="SDA10_000396"
     CDS             complement(474..908)
                     /locus_tag="SDA10_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEG4239328.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            1046..1783
                     /locus_tag="SDA10_000397"
     CDS             1046..1783
                     /locus_tag="SDA10_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4239329.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            1776..2987
                     /locus_tag="SDA10_000398"
     CDS             1776..2987
                     /locus_tag="SDA10_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4239330.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            3191..3973
                     /locus_tag="SDA10_000399"
     CDS             3191..3973
                     /locus_tag="SDA10_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEG4239331.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            4043..4705
                     /gene="trmB"
                     /locus_tag="SDA10_000400"
     CDS             4043..4705
                     /gene="trmB"
                     /locus_tag="SDA10_000400"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEG4239332.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(4803..6557)
                     /gene="ade"
                     /locus_tag="SDA10_000401"
     CDS             complement(4803..6557)
                     /gene="ade"
                     /locus_tag="SDA10_000401"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEG4239333.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            6689..7618
                     /gene="tadA"
                     /locus_tag="SDA10_000402"
     CDS             6689..7618
                     /gene="tadA"
                     /locus_tag="SDA10_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEG4239334.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            7575..8624
                     /locus_tag="SDA10_000403"
     CDS             7575..8624
                     /locus_tag="SDA10_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HEG4239335.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            8621..8899
                     /locus_tag="SDA10_000404"
     CDS             8621..8899
                     /locus_tag="SDA10_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEG4239336.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            8874..9344
                     /locus_tag="SDA10_000405"
     CDS             8874..9344
                     /locus_tag="SDA10_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEG4239337.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            9361..9663
                     /locus_tag="SDA10_000406"
     CDS             9361..9663
                     /locus_tag="SDA10_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239338.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            9680..10051
                     /locus_tag="SDA10_000407"
     CDS             9680..10051
                     /locus_tag="SDA10_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HEG4239339.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            10023..10382
                     /locus_tag="SDA10_000408"
     CDS             10023..10382
                     /locus_tag="SDA10_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239340.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            10554..11561
                     /locus_tag="SDA10_000409"
     CDS             10554..11561
                     /locus_tag="SDA10_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG4239341.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            11586..12770
                     /locus_tag="SDA10_000410"
     CDS             11586..12770
                     /locus_tag="SDA10_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEG4239342.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(13207..14790)
                     /locus_tag="SDA10_000411"
     CDS             complement(13207..14790)
                     /locus_tag="SDA10_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG4239343.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15368..15511)
                     /locus_tag="SDA10_000412"
     CDS             complement(15368..15511)
                     /locus_tag="SDA10_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEG4239344.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(15733..16212)
                     /locus_tag="SDA10_000413"
     CDS             complement(15733..16212)
                     /locus_tag="SDA10_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG4239345.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(16285..16569)
                     /locus_tag="SDA10_000414"
     CDS             complement(16285..16569)
                     /locus_tag="SDA10_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239346.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(16647..16841)
                     /gene="ssrS"
                     /locus_tag="SDA10_000415"
     ncRNA           complement(16647..16841)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="SDA10_000415"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(16913..18199)
                     /locus_tag="SDA10_000416"
     CDS             complement(16913..18199)
                     /locus_tag="SDA10_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEG4239347.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            18337..19008
                     /locus_tag="SDA10_000417"
     CDS             18337..19008
                     /locus_tag="SDA10_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEG4239348.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            18974..19462
                     /locus_tag="SDA10_000418"
     CDS             18974..19462
                     /locus_tag="SDA10_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEG4239349.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            19475..20422
                     /gene="prmA"
                     /locus_tag="SDA10_000419"
     CDS             19475..20422
                     /gene="prmA"
                     /locus_tag="SDA10_000419"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEG4239350.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            20423..21187
                     /locus_tag="SDA10_000420"
     CDS             20423..21187
                     /locus_tag="SDA10_000420"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEG4239351.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            21420..23633
                     /locus_tag="SDA10_000421"
     CDS             21420..23633
                     /locus_tag="SDA10_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEG4239352.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            23644..24090
                     /locus_tag="SDA10_000422"
     CDS             23644..24090
                     /locus_tag="SDA10_000422"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEG4239353.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            24261..25520
                     /locus_tag="SDA10_000423"
     CDS             24261..25520
                     /locus_tag="SDA10_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEG4239354.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(25609..26556)
                     /locus_tag="SDA10_000424"
     CDS             complement(25609..26556)
                     /locus_tag="SDA10_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG4239355.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            26950..28242
                     /locus_tag="SDA10_000425"
     CDS             26950..28242
                     /locus_tag="SDA10_000425"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEG4239356.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            28257..30026
                     /gene="aspS"
                     /locus_tag="SDA10_000426"
     CDS             28257..30026
                     /gene="aspS"
                     /locus_tag="SDA10_000426"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEG4239357.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            30135..31010
                     /locus_tag="SDA10_000427"
     CDS             30135..31010
                     /locus_tag="SDA10_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG4239358.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            31276..33159
                     /locus_tag="SDA10_000428"
     CDS             31276..33159
                     /locus_tag="SDA10_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEG4239359.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(33234..33518)
                     /locus_tag="SDA10_000429"
     CDS             complement(33234..33518)
                     /locus_tag="SDA10_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEG4239360.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(33508..34242)
                     /locus_tag="SDA10_000430"
     CDS             complement(33508..34242)
                     /locus_tag="SDA10_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEG4239361.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            34371..34994
                     /locus_tag="SDA10_000431"
     CDS             34371..34994
                     /locus_tag="SDA10_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEG4239362.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            35186..36307
                     /locus_tag="SDA10_000432"
     CDS             35186..36307
                     /locus_tag="SDA10_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEG4239363.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            36314..39442
                     /locus_tag="SDA10_000433"
     CDS             36314..39442
                     /locus_tag="SDA10_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEG4239364.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            39461..42655
                     /locus_tag="SDA10_000434"
     CDS             39461..42655
                     /locus_tag="SDA10_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEG4239365.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(42801..43508)
                     /locus_tag="SDA10_000435"
     CDS             complement(42801..43508)
                     /locus_tag="SDA10_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG4239366.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14126 a         7773 c         9236 g        12647 t
ORIGIN      
        1 actataccat atcccatttc ctttaaaaac gttgtcgtgg tagtttcaaa caaaattcaa
       61 aatttcatca cattcctagg aaatacgcaa acttttctca cttatatagt taaatctgtt
      121 gagaaaataa tcattattat atttgttcag gatactctgg cagatcagct tttagttttt
      181 ctacttgttc attgatttgt gtgatacgtg gttcggcttg gaatttaaaa tcttcgatat
      241 cttttttgat tgcttcttgg aaagcaggaa tcacagattc agctgtttct tttgtttcga
      301 tcaatgcgct tttaaaatga ttcaggctgt tatttaaatc atttgtcaga gttgttgctt
      361 cttccaattc ttcgatcagt ttctttcttg tttcattccc gctcctgggc gccaataaca
      421 gacctccgac agtgccagca gcagctccaa ataacagtcc tttgaaaaag tttttaatca
      481 tcgcaggaca cctgctttac gattgtttct tgaatagctt ttagttcttc cgatccataa
      541 ctatcttgat gattgacaaa gtgaatagaa aaatcatcct ctttgctgta gcgagggact
      601 aaatggatat gtgaatgaaa aacagattga taggctagtt cacggttgtt attcaagata
      661 ttaagacctt tcatttctgg aaaagctttt tccaatgctt gagcaatctt aggcacacgc
      721 gaaaaaacct ctcctgcaag ttcgggatca tattcaaaaa tatctgctac atgacgttta
      781 ggcacaacta atgtgtgtcc tttcgttgct tgagagatgt ctaaaaaagc atagaccttg
      841 tcgtcttcat atattttata gcttgggatt tcttgattga tgattttgca aaaaatacag
      901 ttttccatat aaaacatcct ttctataaag aataaatact tcagcttaac tttaccataa
      961 gttaaagcta ttgtaaggaa aaaaacaaaa taccgtcatt ttcttcgtgg tataataaat
     1021 aaaaataaat ggcggaggaa aaagaatgag tttaattatc gatcatgtca caggggggta
     1081 cggacatctc cctgtgttaa aagatatcag tttcgatgta cagcctggag agatagtagg
     1141 gttgattgga ctgaacggag cagggaaaag tacgaccatc aagcacatta tcggtctgct
     1201 ggaagcttca aaaggaacga tagaaatcaa tcatctatcc ttaaagaaaa atacggaaga
     1261 atatcgcaaa caaattgggt atattccaga gactccttct ttatatgaag aattgacatt
     1321 gagagaacat atcgaagtca ctgcgatggc ctacgatata cctatggata tagcaatgaa
     1381 aagagcagaa aagttgttgg agacttttcg gttatctaat cgtcttgact ggttcccagc
     1441 taatttttca aagggaatga aacagaaagt gatgatctta tgcgcctttt tgatcgagcc
     1501 ttctttatat atcatcgacg aaccattttt aggcttggat ccactagcta tcaatgcatt
     1561 gcttgaattg atggtagaaa tgaaaaaaga aggagccggt attttgatgt ctacgcatat
     1621 tttagctact gcagaaaaat attgtgatcg ttttgtggtc ttacataacg gagagatacg
     1681 agcagacggt tctcttgaga tgttgagaaa agaatttcag ctgccggatt cttctttgga
     1741 cgaaatctat attgctttaa caaaggagca ggcacatgag tagttttttt caaaaacgtt
     1801 tagcaagaca tcaaaagaaa atgatgaaat atatgcgtta tgtattgaac gatcattttg
     1861 ttttagtttg tcttttcttg attggcggtg tgggattata ttattctaat tggttgaaaa
     1921 ccctgcagcc accgtttgca attggagggc tcataatctc tgtattttgg atgctctgtt
     1981 tattcgtggg taagatggct acttttgcag aggcagcaga tattgttttt ttacttccta
     2041 aagaaaaaga aatgcaaacc tatttggaac gagcttttcg ctattcttgc atttttccgt
     2101 tgatcttttt gatattggca tgcggattgg ccatgccatt agtggtggtt agtacggagc
     2161 aatccttttc gatgtttttc gtctatctga tcatattatg gagtttgaaa ttcagtcatc
     2221 tgcaaatcaa gagagcagaa ttgtttcgac tagatcaaca agttattcgt tattggaaat
     2281 taggctggtt tttttcttct ctgatctttt tgtttctatc attttattgg ctaatatggg
     2341 caggaatagc aggtgcatta gtacaggcaa gtctatatta ctggtttttg tggaagcaac
     2401 aagaaaaagg attggattgg gagaaaatgg tgcatgtaga agaacatagg ttgcatcaga
     2461 tctatcaatt tatcaattta ttcacagatg ttcctgaaat tacggcaaag atcaaaagaa
     2521 gacgctattt ggatcctata ttgactcata ttaaaagaga atcaaaaaac acttatcttt
     2581 acttgtttgc aagaagattg gcaagaggtt ctgattttag tggtctttat ttccgattag
     2641 taattatagg tgggttattg attgccggag tagtcgactt ttattttagt ctgattatcg
     2701 gtttcttatt tttatatttg atcggtttcc aactgttacc tttagcgaac cagttccgct
     2761 atatggttct cgttcagctt tatccaatcc ataagaatca gaagaagcag gcgattcagt
     2821 atctactcac atgggtatta attatcgcgt cggttatttt tggtttgatt gctgcagtcg
     2881 tattagatgg gcagcagcga tgggttccgc tagcggctta tttgattgtt actatgctct
     2941 ttgttaaact atatgtacca actcgtttga agaagatgtc agactgaaaa caaagctgtg
     3001 tagggtaaaa atgagaatga aaaatagagc gttgacagta agaatgctgt tcgttctatt
     3061 ttttttgaga aaagctggtt ttttgaatgg caattaaatg aaaaagaacg tatatgtgct
     3121 tgacgtgtag tgttcaggcg actagaataa taagtactgc aaaacaaaga gtaagaggac
     3181 gggagaatgc atgatggagt ttcagttaga ccaagagtgg cggttacgtc ccatcaaagg
     3241 cgacacagga aaaacataca taggcatgcg tgatgaggac aaagtcttta ttaaacgaaa
     3301 cacgactccg atgttggctg ctctttcaag ggaaggaatt acaccaaaac ttgtttggac
     3361 aaaacgaacg gggaatggcg atactttgac agcacaagag tggctggaag gtcgcttgct
     3421 gactcctaat gagattggtc aaagaaacga cgtgattgat gttttgtacc atttacatca
     3481 ttcaaattct cttaaaacca tgctgacccg tatcggtggt cgtgtcatga cgccggagaa
     3541 gatgctgcaa aaatatgaaa aagaattacc ggaagaactc cagaaaaatc aatttctgca
     3601 aattgtccat cgttttcttc gtgagcagat cccgtcaata gataaggaaa acttatcagt
     3661 agtacacgga gatgtaaacc atcgtaattg gttagtatgt aaaaattatt tatatctagt
     3721 cgattgggat tcaatcatgt ttgcagaccc tgcggtggat attgcaacga ttttaggaaa
     3781 ttatgtgcca ctgtctagct ggaatcagtg gctgatcagt tatggcatac gcccgactga
     3841 cgaaatgctt gaaaaatttc attggtatgc ggccatgaat ttgctccaag aaatcaccag
     3901 atattgcaaa cgtgaagatc atcgacggat gaacgaagaa attttacaat taaaacgaat
     3961 tttcagtggc tgaggtttgg cccaaactct gcggagattt ggccagacct ttttaattag
     4021 aggaaaggaa tggagataaa taatgcgtgt ccgaaatcgt ccgggagcag cggaaatgtt
     4081 agcggctcac ccgaattttg taattagtga tccaacattg tggaagggaa aatggaatga
     4141 attgtttgaa aacgatcatc caatccacat tgagattggt atggggaaag gccagttcat
     4201 cactggaatg gcaaaagccc atcctgaaat caattatatt ggagtagaaa tgcaggtcag
     4261 tgttgtttcg attgcactag acaagttgat cgaacaaccg cttcctaacc tgaaacttct
     4321 acatgtggat ggatcagcgt tgacagagta tttcgctgac agcgaagtag atcaaatcta
     4381 tctgaatttt tccgatccat ggccaaaaaa acgtcatgaa aaacggcgat tgacatataa
     4441 gacttttctt gctgtagatg aacagattct tcgtccaaat ggagaaatcc atttcaaaac
     4501 agataatcaa ggattgtttg agtattctct ggcaagtttt tctcaatatg gcatgatcct
     4561 caaacaggta tggttagatt tacatcaaag tcaatttgaa ggcaatatta tgacggagta
     4621 tgaagaaaaa ttttcttcaa aaggtcaacg tatttatcga gtagaagctc aatttcaaga
     4681 taagaccaaa aagaaaagtc tgtgaaacta actttctgct ttcataaaaa cattaatatc
     4741 cataaataaa tctcagaaaa tgaacattac agatgtaatg tattttctgg gatttatttg
     4801 atttaaaatg tatttatttc atcggttttt ccacaaggtg gaatcaattg gaagcgagaa
     4861 aatgaaaata atcctttgtc ggtgatcttc aattcgggta tgacagataa tgttaagaaa
     4921 gaaagtgtca aaaaaggatc aaaggtttta gctgctccta gttgatgtgc tttagttatt
     4981 aattcattca actgttcata gacgatcgaa aaaggttcct gtgtcatgat tccgcctata
     5041 ggaagagaaa cacaagctat ttcctgctgt ttgttgacaa cggtcatccc gccacctttt
     5101 tgaatcaaaa gattagcagc atacagcata tcctcgtcgt ttgtcccaac gacaactaga
     5161 ttatgcgagt catgggcaat cgttgtcgct atcgctcctt gtttcagctg aaatcctttc
     5221 actatgccta atccaatgtt tcccgtttga tggtgacgct cgatcaccgc aatctttaat
     5281 tggtcttttt cgattgaagg aatgaatcgt tcgccttgtc ggtccacttt ttctaccaaa
     5341 tctttagtca ctagactatt cgggataatt tcgatcacat gggcttgtga agaagaaaga
     5401 agcagctcta acgaatgtgg atttaatggt tgagcgttta gttttggcag acaacacgta
     5461 tatgaatttg aatgatcagc aaataaatgg tcttggagtt gcccagattg aacaactaat
     5521 gttcccttat agaaaacttc atgaattggc agcttggtaa gatcgtctgt taagaagaaa
     5581 tcagcttgat acccaggggc aatcgcacct agtgttttca aatcaaaaca ttctgctgca
     5641 ttcaatgttg ccatctgaat ggcttgtaat ggatcgattc caagtgcaat agcttgacgg
     5701 acgatatgat cgattgatcc ttcttttact aaatcattga tcagtttatc atcggtgaca
     5761 aagaagcatc ttcttgaatt ttcaggtgtg atcgccggta gtagcgatgc cagatctttt
     5821 gcaacggtcc cttctcgaac cattaaatac ataccaagat ccaaacgttc tttagcttct
     5881 tttgcagttg tagcttcatg atccgtccga atacctgctg caggataaac atttaactct
     5941 tccatgccga taccagctgc atgcccgtca atttttttct tatgctggtg catcaattgg
     6001 atcttttcta ggatgtctgt ttcattattt gctactgctt gatagttcat gacttcagct
     6061 aagcctaaaa cttcgggttt ttgtaaaaaa ggggctaatt tttttgcatt taaatttgca
     6121 ccattggtct caaaaggggt tacaggtaca caagaaggta gcataaaaaa gatattcatg
     6181 agtgtttgtc ttgcatcttc tatcataaac tcaagacctg tactcccagc aatgtttcct
     6241 atttcatggg gatctgtcac tacagtagta actccatgaa gcaaggatac ttttgaaaat
     6301 tcgcttggtg ataaaagtga gctttcgata tgcacatggc catcgatgaa tcctggcact
     6361 agatattgcc cattagcatt atgtacgctt tttccttcgt attcaccgat tcctacgatc
     6421 attccttcgc agattgctac atctttttcc atgatttctt tcgtaaagac gttgacgatc
     6481 ttagcatgct tgatcactag gtcagcaggc ttttttttac ttgctacttc gatatatttc
     6541 cttaatgttg tttccaatgg agtgcctccc aagataaatt tttataagta taacataaat
     6601 tcggcttttg ccaatatttt tttgtaaatt cattttattt gaaacaaaaa gatcaaaaag
     6661 acgtatgttt tttgtggagg ggagaataat ggaaataaaa cgattatcgg atcaaatgat
     6721 aagtgaaagt tttgccaaac atgcccaaga cgtatatata ttgccagctt cagaagtatt
     6781 ttacatctat tttcgctatg gaaaaaaacg aacattctgg aagaaagtct cttcggcaga
     6841 tggacagcaa ctgatctcaa gatttaagta tttgggacag atggatatcg gagaaaaaag
     6901 aaaagctcag ttaggtgcat ttacttattc tttagaaaaa caggaagttc gtctgcggtt
     6961 gtcttctgta ggagattacc ttcaacgaga gagtttagtg attcggctat tatacggtac
     7021 ttttgaaaca ccccatcgtt gctttagttc tcgagatttt gcaactattt tacagaaaac
     7081 cgtcagacgt ggattatacc ttttcagcgg gacagtcgga tcaggcaaga cttctctaat
     7141 gtatcggttg gctttggaag aaaaattgca agtgattact atcgaagatc ctgtagagat
     7201 agaagaaccg ttttttctgc agctacagat caatccgaaa atagcacaga cttatgatac
     7261 acttttgaaa ctcgcattac ggcatcgtcc agacttgcta atcattggcg agataagaga
     7321 tgaatcaact gcacatgctg cggtaagagc agcactaaca gggcaccggg tttttgcaac
     7381 cgtacatgca cgcaatcttg aaggtacatt ggtcagaatc catgaactga caaagaaaaa
     7441 agaagagcta tctgaatgtt tagctggtgt catttatcaa gaacttttgg aagatgccaa
     7501 tcaccatcct tctgttttat ggagttatca atttatggag gaagaatcag taaaaaaatc
     7561 atgggaggaa agttatgaag aagctcagga aaatatttgg cagtcagaac tattctaaag
     7621 gattaacaag aagccagcaa aaccagtttg cacaaaccat gggagaactg cttttatctg
     7681 gttttagtct gcaagaatcc ctttatttat tactcacgat ccgttcgttt ccacctgtta
     7741 ttttggcaaa tatccaacat cagctaaagg aagggcaagc gtttagtgag gtactttcgc
     7801 aaataggata tggagcagag caactggtac agatcgagtt agctgaacgt catgggaatc
     7861 tgccgacaac tttgttggaa attgccaaac agtttcggct tgttgaagga tttcgcaatg
     7921 atttgaaaaa gctgcttgca tatccgatgc tattgctcac ctttttgcta ggtatcttgt
     7981 ttagtatgcg gttgttcgtt ttgcctcagc ttattggttc aggaatggtc atgcaagagc
     8041 attggggtat tcagctgata caagtctttc attggtatgt tctactattc atcctagcgg
     8101 ttactctatt ggctttacta gtaaggtatc gtctgatgaa gttgtctcct gttgatcaga
     8161 gtgaatggat aagcagacaa ttattgattg gaccgatata ttccttgtat caatcggctt
     8221 atttgtcact agagattgga aagctttttc atgaaggttt cgaattaagg caaatcatag
     8281 tctgcttaaa ggaaacgaaa caagaaagtc tgattcagca tcttgcctct aagttgattc
     8341 aaggattgga ggcaggtatg tcattggctg agcagttcca gaattatcgt tttctatcag
     8401 aagaattcag taaaattgtt ttacagggag aggcaaaggg aagtttaggt aaagaactga
     8461 ttttttacag cgaatggaca agaaaacagc ttttcaaacg tcttcatcac tggatgcatt
     8521 ttattcagcc aatcattttt ttgggcgtag cggctttgat tttattagtt tatgccgctg
     8581 ttttattacc tgtttacgga agcatagagg aggttttgcc atgaaaaaga gaaaaatatc
     8641 ttattatagc ggttttacat tgatcgaaat gctaatcgtc ttactgatca tcagtgtatt
     8701 agtgttgttg ttcgtcccta atttgtcgcg ttataggaat catgtagacc aagaaagtcg
     8761 tgaagcgatc atccagttgg tagacacaca aaaagagctc tatgcgcttc aaaataatgg
     8821 aagagttcct actgtggagg aattgttgaa tgaaggctat atcaaacgag aacatgcaga
     8881 aatctatcag cggccttgaa ggtaaggttt tagcatcagg atatacgttg tttgaatcac
     8941 tcttactgat tttattactt acaacgatcc tttcttttcc agttctcgca ttttctgcat
     9001 ggaagaatga attggcagtc tatcaatttt tcggccaatt tgaaaaacga atctattcta
     9061 cgcaaaaaat cgctattgtg aatcagatcc aaacaggctt ttattggaat agtgaagaga
     9121 atcaaatcat ttttcatgtt ccgaatccag ataaaataaa ttgggaagta ttagatattc
     9181 ctgatgagat taccttgaaa cgccatgctt ctattacttt tgctgctaga accgggaatg
     9241 aaagttcact aaaagcttat caattttatt gggaagaaaa agagcggacg attacgtatc
     9301 aattccagat agggagtggt cgctatacaa aaagaattga ataaaacagg atttgttttg
     9361 ttggagtccc ttacagcgct tatgatcgct tctgtctgtg tctacttagt gagctttggc
     9421 cagctgtttt tgcttcaggc agaaaagaaa agtggggagg aactgctatt tattcgaatg
     9481 atttatgaag atatcaagac gcagcggatg tatcacaaaa gaccagagcg aagggaagaa
     9541 atgatcccat acgatatcca aattgtcgat aataatggag taatcgaaaa agtgtttgtc
     9601 aatatggaga aagaagcgat cagtattgaa aaaacaagta attccagcat ttacgctgat
     9661 tgaatgtcta attggactga tgatattgag cagtctattg ctcacattca atcttttgat
     9721 tcagcaaacc atccaagtaa gtgaattttt gcagcgtaaa gatcaaaaag agtggctaat
     9781 atttttagca caattagaag aagaactcaa gagcagtcac aatgtctctg tgaaaaatca
     9841 gcaactgcac tacacgatcg aggataaaca gtatatcatc gaaagatatc aggcaatgat
     9901 acgtaaaaga agaacttccg gcggacatca acctatgtta acaagtatca ctgagcttca
     9961 attattggaa aaagaacaca caatttcttt ttatgtacac tttgaaaatg gagaagatgg
    10021 atatgccata tggacaaaaa acgatcagta gaaaatgtta taagggctct attttatttt
    10081 ctgtcttgct tttatttctt ttatgtacgt ttttattgat ggcagcatta gaaagctacc
    10141 gtttgtcaat ggatcttaat atacgaatca aaaattatta tatagcaaaa ataatgaagc
    10201 agatggcttt atcggaacag tctacgcttg ccgaaagttc ggaaggagtt ttttactaca
    10261 caactggatc tgtaacatat cagtgggtac aacagtccct attcttagag gtggaagtct
    10321 caccgtttat ctttcggttc acagaagaag taaaaaatga tacagatact agtacagaat
    10381 gatggaatgt tcgtgaaaac gccctctttt ctgttataac actgttttta ataaaaaaat
    10441 gtttgccgtt tcacaaataa aaaacagtga aaacgattga aattttgtca attgttcgat
    10501 agaattaaac aggtgaaaaa attgttataa tatatctaga aatgaggtgc accatgttac
    10561 cagaaaaaat ggaacaaggg tttaatcaaa acctggaagc tattcagctt ttacagaatg
    10621 cattgggaac ttccttttta gaagcctatg tcgaaaatgc agaaaattta atagatgatt
    10681 atcaagtacg tgtggtggat ggagtgccga caaaagagac aacgcaacgg atcacagctc
    10741 tttatgaaga gttgaagaaa ctttctttcg agccagaaga atggcgcaga ctgtctcaat
    10801 tacttttatt gaagggcagc cagacagaac atttgcaggc gaaccatcaa ctcactcctg
    10861 atagtatcgg ttttttattt gtttttctta ttgaacaact atacacaaat aaaaaagcac
    10921 cggttaagat tctggatatt gcggcaggaa tgggcaactt gttattaaca gttcttttaa
    10981 acctatcgaa tgcggggtac caaacagaag gaattggtgt ggatattgat gatacattgt
    11041 tggctgtcgc agcttctaca agtgatttga cacaagcaaa tgttcaatac tttcatcaag
    11101 acgggttgca ggaattatta atagatcctg tcgattttgc aatcagcgat ttgccaatcg
    11161 gttattatcc gaatgacgaa aaagcaaaag agtttttaac aagtaccgaa gaaggccata
    11221 gttatgcaca tcatttattg ctagagcaat caatgaaata tgtgaagccg gatggttttg
    11281 gtttgttttt gatgccgagt ggatttttag aaacggatca aagtgaagaa atcaaaaaat
    11341 ggtttaaaga agaaggttat cttcaaggaa tgattcaatt gccagatgaa ttgttccgta
    11401 ataaacaatc gcaaaaaagt attttgatct tacagaaaaa aggaccacaa gcacaacaag
    11461 taaaagaagt cttgcttgtg aaacttgctt cattaaaaga accagagaag gtgacggaat
    11521 ttttcaacga gtttaaaaac tggaaatcat ctgctctgta atcattcaac gagaaggaga
    11581 gtcatatgtc taaaacaatt gcaatcaatg ctgggagttc aagtttgaaa tggcaattat
    11641 atcaaatgcc agaagaaacc gtagttgcga aagggatcgt cgaacgtatc ggtttacaag
    11701 attctatttt taccatcaag tacggcgata atcaaaaatt cgaacaaatc atggatatcg
    11761 aaaaccacga agtagcagta aaaatgctgt tagatcagtt gattgaactg caaattttaa
    11821 gctcttatga tgaaatcacc ggagtagggc accgtgttgt tcacggagga gaaacttatg
    11881 gggattcagt agtgatcgat gagaaagtaa tggaccgcat tgctgaatta gcagaatttg
    11941 ctcctttgca taatcctgca aacttaatgg ggatcaaagc gtttaaaaag attttacctg
    12001 acattttgag tgtggctgta ttcgatacat ctttccactc aacaatgcca aagcaaaatt
    12061 atttatatag cttaccggtg tcttattatg aagatttcaa agtaagaaaa tatggcttcc
    12121 atggcacaag tcatcgttat gtgtcggaac gtgcggcaaa attattgaac aaaccgatcg
    12181 aagaactaaa aatcatcact tgccacttag gaaatggtgt atcgatcaca gcagtcgatg
    12241 gaggtaaatc catggatact tctatgggat tcacaccatt agctggggtg acaatgggta
    12301 cacgttcagg tgatatcgat ccagctgttc tcccatattt aatggaaaaa ttagagattg
    12361 atattgatga aatgatcaat gttttgaaca agaaatctgg tctgctaggc ctaacaggat
    12421 tgtctagtga tatgcgtgat cttgaaaaga actatgaaaa agaacatatt cgtttagctt
    12481 atgacatttt cgtggaccgc atccgaaaat acatcggcgg ttatgtgaca acgatgaatg
    12541 gtgttgatgc aattgtcttt actgctggta tcggcgagaa tgacggacat gtacgtagtg
    12601 aaatcatcaa aggtatgtct tggtttggtt gtgaaatcga tccagcgttg aatgaattgc
    12661 gtggtgaaga agtagatatc tctacaaaag aatcaaaagt aagagtatta gtgatcccaa
    12721 cagatgagga actaatgatt gcccgtgatg tggagcgtct gcgcggctaa gccaaagcat
    12781 tcgttaggat aaaagatgaa acgcatgcaa gagggaattc ttgcatgtgt tttttttgcg
    12841 gagaagaccg caaaatggta atttaacttc aatttccctt cttttttaca ggatcttctt
    12901 agggaatttt gcttggaaaa gtggtcaaaa gggaaaagac tggcatttta tttattgatt
    12961 ttcaggaaaa tcccaatatt ttattttcat tcatctttat aagggacatt acatatgtaa
    13021 aaagaaaaaa catttttaga tcagcctcgt gggctgtcga ctttattctt cgctttatgt
    13081 ttagctagaa aaccgatccg aaacattcat tgatcgaata ataaaaactt gattttccac
    13141 ctgtactttg cctgaatcgt gaaagcacag gtatgaaaat caagtttttt gttgcgaaaa
    13201 aacgtcctag tcaatcagtt ggccgagcgg ctggcggctg atctgctcct gatcaataac
    13261 gagtgcttct tggaagtgtt gctgcaaaaa gtagcggtca tggctgactg ctaggagcgt
    13321 tccttgatag tcttgcagca tttcttcaat ttcttctcga gcatagctgt cgaggtgatt
    13381 ggttggttca tccaaaatca ggacgttgat tttttctaaa aagagtttca gcaacgccaa
    13441 acggattttt tcaccaccgg ataaatcttt gatccgttta cccacatcgc tttgataaaa
    13501 gccacttttg gctaattgcc gtcgagcgac ttcttcgttt ccagtcaatg tgatggcgta
    13561 agccagcaaa cggctatcgg gatcggcaaa ctgcagctgc tgcggcaaat aaccgatttg
    13621 caagctacta cctaatttga tggtgccacc gtaaggcttt tcctcaccga gtaataattt
    13681 gagtaaggtt gattttcctg caccattgtc gccgatgatg gctaaatgat ctccacgata
    13741 gactgcaaaa ctgctgtctt tgaatagtac ttgatcagcg tactgctggt gcaagttttg
    13801 aatgatgaaa acttctttgc cagattttcc accattagac aagctttgca gccgattttt
    13861 cgggggaggt ggcggtttga cgagtgtcag tttcgccagg cggcgttcca gttctttggc
    13921 ttttttgaag aaggactcat tgtctccttc atgtgcccac tgacgaaaac gacgaatcat
    13981 gactttcaac cgctggactt ctttttgctg cagttcgtaa ttttttgtca gctcagccaa
    14041 gcgagcttct ttcaagactc gaaaacggct gtagtttccg ggatactcaa tcaatgcacc
    14101 ttcttccagt tcccatgttt ttgtggtgac ttgatcgaga aattgccgat catgagaaac
    14161 gaccagaacc gatttttttg tattctgtaa atagttttcc aaccagcgaa tactgttaag
    14221 atctaaatga ttggtcggtt catcgagtaa aagtaaatcg gcatcggctg ctaaaatttt
    14281 ggctaaagcg actttaactc gttcgcctcc gctaagttct gttaaccgtg cttcctgaca
    14341 gtgtggaatc cctaagcctt gcaagatcgc tgcaatccgg tcttctaata cgtaaccgcc
    14401 tagttcttca aagcgatgct gcagctcacc gtaacgggcc agacacttct ctaagtttgt
    14461 atctggttct gtcatcaatg cttcgtagcg actcatttgc tgtttgaggt cctgtatctc
    14521 agcggcagaa tggagcaagt agtggttgac ggtcgcttcg gaaactgtaa gttcttgttc
    14581 gaccgtcccg atctttaagc cttttttgcg actgataaca ccgctgtcga caccttctct
    14641 gccggtcaag atttgtaaaa aggttgattt gccagttccg ttgatatcga ctaaggcaat
    14701 tttttcacca gcttcaattt gacaggataa atcctcaaat aatggattcc cgccgattgt
    14761 ttttttaata gaaattgctt gtatgatcat gatctctctc cttgagacca ctcaaaaagg
    14821 ccatggttat ccctggcctg tgttatttaa actcagatta tcatgagaaa aataccttga
    14881 gatggccatt gattttacgt gaattcaact gttttggaca aacttgtccc gtcaacagca
    14941 actgtaaaac caattgcatg gctaaatgcg atatggtatc gttggtaatt ttcattactt
    15001 gttattttag tcatgcgcat catagtattt tccctccttc gtgtagttgt cttttccagt
    15061 gtacctgaaa gtgtcagaaa gttcaactag cgctgacatt tacttaatat ttatcgatgt
    15121 gtaattctgt cagagagctt tttttatctg actgagggta atgtctcgtg gaccacatca
    15181 ttgtattctg agctaaacca gcgttcaggc gaaatcatat ggtgtttgat aaaaaacttt
    15241 gtctacatga cttccttttg ccatttgctt aataccgttg cattaaacag ctcttctctt
    15301 tggaaatctc accaacatcg cgttcaggag tatcgttgtg gttgcaatgg caagcggtag
    15361 cctttcatca attcttgaat gatttaccga atgtagcact tgccttctcg cggtacccga
    15421 acccgaaata aaattttagt ttggcgataa aatcggtatc cgccatcgat acttcatatt
    15481 ttttcgtatc agattcatcg gaaacttcca cattgacgaa ccaataagct ttggcatgct
    15541 taggtcgttt taaaccacac cagggcgact aatcgccctg gtgttcactt tttttatttt
    15601 acttgaactc attgactgtt ttccctttat tttaaacatt ataaagaccg atgagtgctt
    15661 gccgcgtctt ttgtttttat cacggttttt tgtgcaaaaa aacctgccga tagttggcag
    15721 gtcgtctacg tgttaagctt tggtgtggtc tttagctggc aattggtttt ctaaatcagt
    15781 tcggacaaca agcacatcgc aagctgcgtt tctaataaca tattcagaaa cagaaccaat
    15841 aaataaacgt tctacagcat tcaatcctgt ggctcctaac ataatcaaat ctacttgatt
    15901 atcttctggg atttctctgg caataatttg ttttggtgag ccatattcta cgacagaggt
    15961 tacattattc aagccagctt tcttcgcatt gctttcataa tctgccaatg tttgctttgc
    16021 catttcagta gcttgttctg caagcattcc atcaaaggat gaaacggttt ggaaagaacg
    16081 tgtgtcaatg acatgtgcta atagaagttc tccgttattt cggatagcta cattgaccgc
    16141 ctttttaaaa gctaattcag cttcatcaga accatctacg gcaaccatga tttttttgta
    16201 ttgttgtaac ataagcattc actcctcaag tcaatcgtat tgtactcttt tattgtagaa
    16261 cactttaggg aaaaagagaa ttatttactg ctttggttca gcaatttttt ttgcaaatcg
    16321 gatactgaag gtggcagaaa caataattac gatcagtaaa aagaccaagg caataaagcg
    16381 ttgattgaaa aaagctgtta aaatacccat tacgcctaaa acagcatatg cagcaccgta
    16441 aaattgtagg aatccttgat ttttatcatt tttgttgatc aatacaaaaa agatatcctg
    16501 tttattaaat aaataatagc taacgaataa taatactaaa gtggcgataa tcattaaaat
    16561 cctaatcatg aacaaatcct ccaaaataag caacaaatag aaaaagcaat ttgccaagag
    16621 caaattgctt tattctttaa aagatggtaa tctccgttaa tgccgttgtt tgtcctgtag
    16681 tattgatccc gcgaattcaa gcaggtgggt cccatggtcg tagcttcgaa ctaccaaatg
    16741 ataaggcatg ttaccagcag tgacacaaga cgggtcccaa gatatcaata aaaatgttcg
    16801 gtcaacacac aaagaggaca attcgaacat cacagatttc ccacctctat agtagcatat
    16861 gaatttaaaa agagcaatga gagagctcgc agaaacagaa ccgttttctt gtctagtttt
    16921 ttggaggatg ctttttccac tcttttatac ggtaatactg ttgacccaga gcctgttcat
    16981 atttccctgt atcttttggc tgataatact gagcattctt cagtttatct ggtaaatatt
    17041 gctgattgac ccaagcctga tcaaaatgat gaggatactg ataacccacg ccacggttga
    17101 gtgatttcgc acctttgtaa tggctatctc tcaaatgatc aggaacatct ccagctttgc
    17161 cctctcgaat atcagcaagt gctgcatcta atgccatgta ggcagaattg gattttggcg
    17221 acaagcaaag atcgacgacg acgtctgcta gtgggatacg tgcctcaggc aatcccaatt
    17281 tttcagcagc taatacagcg ttcactgtac gagccgcagc agcaggatta cctaaaccta
    17341 tatcttcata accgataacc atcagacgcc ggcaaatact tgccagatca cctgcctcaa
    17401 ctaaacgggc gagataatga agtgcagcat ccacatcact tccacgaatc gatttttgaa
    17461 aagccgaaat aacgtcataa tgagcatctc catttttgtc atgggtaaga gcttttcttt
    17521 ggacacattc ttcaatgatc gacagtgtaa gatgaatcct tccttcttta tctgaaggag
    17581 tcgatagtgt agccagttcc agcccattca atgcactccg caaatcgccg ttcgttgcac
    17641 gcgaaaggtg aagaagagca tcttcgtcta actggattgc ttgttgtcca aggccgcgtt
    17701 ctttatccct taaagcatgt tcgactgcca actggatgtc ttgttctgtt aatggtttga
    17761 cttcaaagat ctgtgtacgg ctgcggatcg ctggattgat ggtaatataa ggattctcag
    17821 tagtcgctcc gatcaagatg attcggccac tttctaaatg aggaagcaga aaatcctgtt
    17881 ttgttttatc taatcggtgt acttcatcca ataagaggat cactgtacca ctcatttttg
    17941 cttcttccgc tacaacttgt agatcctttt ttgaatctgt agcggcgttt agcatgcgaa
    18001 atgcataatt agtagagccg gcgatcgcac ttgcgatact cgtttttcca gtaccaggag
    18061 gtccatatag aatcatggaa gagagcatgc gagcatcgac catgcggcgt atgatttttc
    18121 cttccccgac tagatgctgc tgaccaacga cttcatcgat cgttcgaggt ctcatgcgat
    18181 aagccaatgg ctgttgcata ggattttcct ccttcttgtt tttacgtccc ttaattatat
    18241 catatttagc aagatgccta gaaagaacag ccttctgttg ttttgttttc atagcttatc
    18301 caactaatat tgtgtatgat aaagtaggtg agaaaaatga cggataaaca aaagatagat
    18361 gaattttttt ggaataaaac gacgccagaa gtggcacggg atttattggg gatgtatttg
    18421 gagcatgaca caccagaagg aagattagct ggatatattg tagacgcgga agcataccta
    18481 ggtccagaag acgaggccgc ccatagctat ggtttaagaa gaacaccgcg tgtgcgggca
    18541 atgtatgaaa aaccaggtac gatctacctt tacacgatgc atactcatcg gattttgaac
    18601 atcattactc agccagaagg gattcctcaa ggagtaatga tacgtgcaat cgaacccgcc
    18661 gcaatgattg atcaaatgag taaaaatcgt ggcggaaaaa ctggtccaga tatcagcaac
    18721 ggtccgggaa aattagtgga agcattagca attcctcaag aactttatgg tcagtctata
    18781 gcagatagtt cgttacgctt agtttttgag aaaaaaaaga caccaaaaaa gatcaatgct
    18841 ttgcctagaa tcggtatccc aaacaaaggt gtatggacag aaaagccgtt gcgttttgtt
    18901 gtgtcgggaa atccttatat ctctttacaa agaaaaaacc aaatcgagaa agattggggt
    18961 tggaggaaag aaaatgaaaa agaaggatct attaacatat ttagatgaaa taattgaaaa
    19021 acaagtgaca gactatgatg tagctttaga ttgggataca aaaaatcata cgatcgagat
    19081 cgttgttcgc ttgtttgcag aaaataaagc acaatttgag attgatgatg cagaagggat
    19141 cgtttcgcaa gaaccaatta tcgaatttga agacggcgta cttcttttta atcctcagaa
    19201 atctgttttt gatgaacgca attatctagc agtaatccct tatgaaggaa aaaaagggtt
    19261 ggcaaaatca gtagctgatt ctttagtgga atatctaaat gaagtattag ctcaagggca
    19321 aagcgatttg cttgattttt tggatgaaga cgatgacgaa gctgtattcg agcttcactg
    19381 ggataatcaa caactagcag aattagtaga agtaaaacag caaaatgaag cagatactta
    19441 tttaccgtac ccaagttatt aaggagtgac agatatgaaa tggaatgaag taaaagtaga
    19501 aactgaaagt gaagcagtcg aagcagttgc taatattttg atggaggcag gggcgagtgg
    19561 tgtcgcgatt gaagattctt tagatgttga acattttcag tcggatcaat atggtgagat
    19621 tttagataaa aatgatttta catcgctgaa agaaggcgca ctagtcatgg cttattttcc
    19681 ggaaacgact ttcttaccgg aaatcttgcc ttttatcaaa gatcgtatca cacaattacc
    19741 tgagtttggt ctagctattg gaaaaaataa agtaacagtt agtgaagtag aggaaagcaa
    19801 ctgggcaaca gcctggaaga aatactatca tccggtgcgt attacgcgaa tgctgacggt
    19861 tgcgccaagc tgggaaaact accagacaga agatcctcta gaaaaaatta tcacactaga
    19921 tcctggtatg gcatttggca caggcactca tcccacgact tccttgacat tgacagcact
    19981 agaaacagtc ttgcgtggtg gtgaaacatt gctagatgtg ggaactgggt cgggcgtgct
    20041 aagtattgca gcaaaatatc ttggtgcaaa agatgtctat gcctttgatt tagacgaagt
    20101 agccgttcgt tcggcgaaag aaaatatgga tatgaatgaa atagccaaag atgtccatgt
    20161 atcagctaac gatcttttga caggaatcga gattgaaagt gatgtaatcg ttgcgaacat
    20221 tttagcagat atcatcttat tgatgatccc agatgcttgg cgtttactga agcaaacagg
    20281 cacgctgatc gtttcaggaa tcattgaaga gaagaagcaa atggttctgg atgccatgtt
    20341 agaacaaggg tttatcgtgg atcaaatttt ccagcaaaaa gactggtatg caattatttt
    20401 gaaaaaacca gaggtggaat agatgcagcg gtatttttta aaagaatcat atgaatcaaa
    20461 agactatttt gttttagaag gggaaaacta tcaccacatt gtacgggtta tgaggatgga
    20521 acccgaggat gaagtttttt tggcattcaa tgatcaaata gccattgttg cagaaatcac
    20581 ggagattact gaagaggctg tttatctgaa agaaattaaa aaagaagaaa atcaaaagga
    20641 actgccaagt tcgattacca tagcgagtgg ctatcctaaa ggggaaaagc tggattggat
    20701 cgttcagaaa tccactgaat taggagcgca agcttttctt ggtttcccgg catcagcctc
    20761 tgtcgttaaa tgggatgcaa aaaaacgtat gaaacgacaa caacggcttg aaaagattgc
    20821 tcaagaggca gcggaacaat cccatcggca gatcgtgcca tcagtagagt tattggaaac
    20881 gaaacaagat ttatttgagc ggctagatga ttatgacgtg gtcttggttg cttatgagga
    20941 atcagcaaag caaggcgaaa aatcaaatgc agccaaaatt cttagtgaaa caaaaccaga
    21001 tagtcgaatc ttagctgtct ttggaccaga aggtggatta tcccgcgatg aagtcgatta
    21061 ttttgtagaa cacaaaggga tactttgtgg attaggacca agaatccttc gtacagaaac
    21121 agcacctctt tatttattaa gtgctgctag ctatcaatgg gaattaatga atccttcttt
    21181 cagctaacgt tcttcattaa taaaagagta tcgacagaaa gaattgaaaa aatagtcaac
    21241 aatttgtata atagaaacaa ttatttatac aatggatacg aaaaatcgtt tgaaaaagct
    21301 tttaaaaagc gaacatttcc gtaaaaaaac gtataactgc acggattttg ggacaattat
    21361 caaacagata tttgtcccga ttcccgtttt accgaaaaaa gaaaggggga tcgattcctg
    21421 tgacaaaaga agaaatttta acgggacccg gtgtcataaa gctcgttagt caatacatgg
    21481 gtcccgaaca tgtagcattt gttgagaaag catgcgaata cgcgactgct gcacatgacg
    21541 gacagtttag aaaatcaggc gaaccctaca ttattcatcc tatccaagtc gcaggtatat
    21601 tagcagattt aaaaatggat ccccatacag tggctacagg cttcttacat gatgttgttg
    21661 aagatacaga aatcacttta gaagatctga gagaagaatt tggcgatgac gttgctatgt
    21721 tagtggacgg cgtaaccaaa ttagggaaaa taaaatataa atcccacgaa gagcagctag
    21781 cagaaaacca ccgaaagatg ctgctagcaa tggctcaaga tttacgagtt atcatggtca
    21841 aattagctga tagactacat aacatgcgta cgttgaagca cctgcgagaa gataagcaaa
    21901 gaaggatcgc tcaggaaact ttagaaatct atgcgcctct tgcacatcgt ctagggatca
    21961 gccggatcaa atgggaatta gaagatacag cacttcgtta tctaaatcca aaacagtatt
    22021 accgtatcgt ccacttgatg cagacgaaga gagaagaacg tgaaaaatac gtaagtggta
    22081 ctgttgaaga tattcgaata gcgacagaag agctggggat ttttgcagaa atctatggac
    22141 ggccaaaaca catttattca atctatcgta aaatgaaaga tcagaaaaag cagttcaacg
    22201 aaatttatga cttgctagct attcgagtga tcgtagattc aatcaaggat tgttatgctg
    22261 tattaggagc aatccacaca aaatggaaac caatgcccgg cagatttaag gattatattg
    22321 ccatgccaaa agctaatatg taccaatctt tgcatactac cgtgatcggt ccggcaggaa
    22381 atccggtaga aattcaaatc cgaacacagg aaatgcatga aatcgctgaa ttcggggttg
    22441 ctgcacactg ggcctataaa gaaggaaaaa atgaaaaagt agaaccagat ggtatgacga
    22501 aacaattaag ctggttccat gagatactcg aacttcaaga cgaaagctat gatgcttctg
    22561 aatttatgga aggcgtaaaa ggagatatct ttagtgataa agtctacgtc ttcacaccaa
    22621 aaggagacgt tactgagtta ccaaaaggat ccggaccatt ggactttgca tacagtatcc
    22681 atacagatat cggtaacaaa accactggtg caaaagtaaa tggcaaaatg gtgcagcttg
    22741 attacaaatt gaaaaacgga gatatcattg agatcatgac ttctccaaat tcatttggcc
    22801 caagtcgcga ctggttgaaa ttagttgcta ctagcaaagc aagaaataag atcaaacgtt
    22861 tcttcaaagc ccaagatcga gaagaaaatg tgatcaaagg ccacgaatcc gtggtcaaat
    22921 gtattacaga tctaggattt acgcctaaag atattttgac gaagaacaaa ctgcaagaag
    22981 cactcgatcg ttttaattat caaacagaag atgatctcta tgcagctgta gggtatggag
    23041 aagttagccc cttgacgatg gccaatcgtc tgactgaaaa agaacgtaaa gaacaaaaaa
    23101 tcgagcagca aaagcaagaa gcagaagaaa tcatgaatca gccgaaaaaa gaacctgaca
    23161 aaatgaaagt acgtcatgaa ggtggcgttg tcattcaagg ggtagaaaac ttactaattc
    23221 gtatcagtcg ctgctgtaat ccgattcctg gtgatgatat cgttggttat atcactaaag
    23281 gcagagggat atccattcat cgtcgagatt gtccgaatgt tcagcctgac aaaccaaatg
    23341 tagcagaacg tttgattgaa gtcgaatggg aagatacatc gaatacacga aaagagtatg
    23401 atgcagattt ggaaatttac ggctataatc gttcaggctt attgaatgat gtacttcaaa
    23461 cagtcaatgc gctaacgaaa aatctcaaca gcgttgaagc acggacgaat aaagataaaa
    23521 tggcgacgat ccatttgacg gttggtatcc agaatttatc ccacctaaag agtatcgtgg
    23581 ataaaatcaa agcagtacct gatgtctaca gtgtacgccg gacgaatgga taggaggaca
    23641 gatatgagag cagttgttca acgggtaagt aaagctgctg tgtcagtcga tcagcaagaa
    23701 attggaaaaa ttggctacgg cctacttgtt ttattaggca ttcatgaaac cgatacgcaa
    23761 aaagatgtgg actatctggt aaaaaaaatt gcacaaatgc gtatttttga agatgaacaa
    23821 ggaaaaatga atattagtgt agaagatgtg ggaggacaaa ttttaagtat ctctcaattc
    23881 acactgtttg cggatacgaa aaaaggaaat cgaccaagct ttgtggaagc cgcacgacca
    23941 gaaacagcta ttcctttata cgaatcattc aatgaaggaa tcagaagtcg cggcatcaca
    24001 gtcgctaccg gacaattcgg agcagatatg tcggtttctc tcgtcaatga tggacctgta
    24061 acgatcatta ttgattctaa aaacaaataa agtaatctgg ttttcactag taaaaactat
    24121 gaaacacgtc gcttaagtta gactgataaa ctctaactta agtggctttt ttgtctgcaa
    24181 aaagcgcttc ttttgtcagc agtcggaaaa aaagttaagc ttaaattata caagttattt
    24241 tttataggag gttgctgaag atgaaagaag taccagtagt agatatcaaa atcacagatt
    24301 taaaaaaatt gtatcaaaaa ggatacaaca tgttggagga gttacgccat gaagcggacg
    24361 ctcccgtcgt caaagcaaag attttcaata aagaagctat cacgatctac ggatcatcag
    24421 cagcaaaagt attttatgat ccaaggaatt tcaaacgaaa aggtgctatg ccaaaattag
    24481 tattgaagac actttttggt caaggtggag tacagacttt ggatggagct gctcatcacc
    24541 atcggaaaaa tatttttatg gatctaatga caccggaaag aatggaagat tatcatcgta
    24601 tcttagataa gaatctgaca caagcacttg aagcacagca tggtcaattt gaactatttg
    24661 atttaagtaa gatggttttc tttacatcta tttgcgagtg ggcaggtatc aatttatcag
    24721 cgatttctaa agatgaggtc gaaaaattag cagaatatca aatctctatg atcagcggaa
    24781 cgtttacttc tccgattgat cacataaaag gtgtcgagaa tcgcaaaaaa tcagaaaaat
    24841 gggcgcaagg cttgattgaa gaagctcgac aaaatcctgt ggctggaaaa gaaaatgtgg
    24901 cattatatgc ttttgccaat gccacagatt tagatggtca gcttttacct ctggaagtgg
    24961 cagctgttga attattgaac atcatccgtc caacggttgc tctaactgtg tgggcggcat
    25021 tgatgggaca tgcacttttc agtagaccag atttgtatca gcaattaaaa aatgactttt
    25081 cgacgttaca agatccgttt attcaagaaa tgagacgcta ttatcctttc ttcccaatgc
    25141 tacctgcgat ttcgctgaaa gaagtagaag tagatggtta tcgtatacca gaaggcagtt
    25201 gggttatttt agatttatat ggaacagacc atgatgaacg aacagttgaa gcgccagatt
    25261 cctttatgat caaacggtat gtagggaaag caaaagatat ttcctataaa gaagaatatg
    25321 agatgattgc acaaggcggg ggaaattttc gacaaatgca tcgctgtgca ggagaatgga
    25381 tcactcttca tagtctacgt gtgttttccg atcagctagt aaataagttt gagttctctg
    25441 tacctgaaca agattggacg attccattca atcagtttcc gacttatccg aatagccgag
    25501 cgctacttta caagaattag attagcataa acttatcgaa aaaaaggaag atcaaacagt
    25561 gctgatcttc cttttttcat attactataa attgataagg tcaattgatc aaaatccacg
    25621 aaatgcttca tcaatggttt gataatcttc attggataaa tgcaaatcta aagcttggac
    25681 attgtcagat acttgctctg gacgcttagc tccaggtata actacagaca ctcgtggatt
    25741 cttcatgtac caagctaaaa caagttgcgc aggagtggct tggtatcttt tagcaatggg
    25801 gcgaagtaca tcgacagcgg ttaaaatttc tctgaaacgt tctccttgga aatctggatt
    25861 tctttttcga ggatctcctt caccaaattg tttttcctca ccaagttcat attttcctgt
    25921 cagcaaaccg gaagcaagag gaaaataggg aacaaaactg attttgtttt tttcaagata
    25981 agggaataat tctttttcag cctgccgatg gatcaaactg tatttatctt ctactacatc
    26041 gacatatcca tctgcattcg cttctttcaa ttgttctaac gtgaagtttg acacaccgac
    26101 tgctcggatt tttcctgctt cttttaattc gtgcaatgta gcaactgatt cattttttgg
    26161 tgtgctttca tccggaaagt ggatataaaa aatgtcgatg taatcagttt gcagacgttt
    26221 tagtgcatct tccaccgatt gtttgagaaa ctcaggagaa ttatcaaatg ttcgcccttt
    26281 atttggtaca tgagcagctt ttgtagcgat gacgacacgt gagcgatcat attctttttc
    26341 cttcagtacc tcgccaatca gttcttcgga acggccgttt ccgtaagcat aagcggtatc
    26401 gatcaaattg atcccgctat tcaaagctgt acgaactact tgttttcctg tctcatcttc
    26461 caaaccgtca aaaagattat gtcctccaac ggcatttgct cctaatccaa gctgttctgc
    26521 gtagacttgt gaatgtccga ttcttatttc tgccatagct gattcctcct ttttgtctag
    26581 tctaaaacaa aataccaaaa agctgaacta aaacgttttt gtgaggggaa atagtcagat
    26641 atctcttgac tttgagactg gtttttagta ggttataagt aatatcgaat gtcaatgaaa
    26701 gagcgagtaa gtttttgaaa ctttacaaga gaggatcatc gaggctgaaa atgatctaaa
    26761 gtttcattag cctaaagaca ctctggagat ggacgagtga aaagccgtcc cgcgtacgag
    26821 cgttattcgt aagaggaaag agaaatcttt caaaattagg tggtaccgtg tatgaatttc
    26881 attcaccctt gttcatgatg aacaagggtg atttttttat ttacatagct aagaaaggga
    26941 gagaaaacta tgagtttcca aagaccaaaa ggaacaaatg atattttgcc aggcgaatca
    27001 gaaaaatggc agtttgtcga agaaacagca cgtttattat tcagagatta tcagtataat
    27061 gaaatgcgta caccgatttt tgaacactat gaagtgatat cccgaagtgt aggggataca
    27121 acggatatcg tttcaaaaga gatgtatgat ttttatgaca aaggggaacg ccatgtgacg
    27181 cttcgaccag aaggtacagc accaatcgtt cgttcttttg ttgagcataa attgttcggc
    27241 ccagagtatg cgaaaccgtt caaaactttt tatatgggac caatgtttcg ctacgaacgc
    27301 cctcaaaaag gacggttacg tcaattccat caaatcggag tagaagcatt tggatcagaa
    27361 aaccctgcaa ctgatgtgga gagcatggtg atggcacttg atttcttcaa acagttgggg
    27421 atcacgcaaa tccggctagt catcaattca ttaggtgata aagaaacgcg tcaagcctat
    27481 cgtcaagcat tgatcgacta tcttttacct tttgaatcag agctgagtga agactctaaa
    27541 cgccgccttc atgaaaatcc attacgcgta ttggacagta aagacaaacg agatcaaaaa
    27601 tttgtggcag aagctccatc tatcttagat tatctaagcg agcctgctca aaaacatttt
    27661 gataccgttg tttccatgct ggatgcattg gctgttcctt atgaaatcga cagtaatatg
    27721 gttcgcggac tagattatta cacacatacg atttttgaaa tcatgagtga cgcaccaaaa
    27781 atgggggcac aagcaacgat ttgcgcaggc ggacgttatg acaatctagt ggaagaacta
    27841 ggtggtcctt caacacctgg cttcggattt gcaatgggta tcgagcgatt actcttgact
    27901 atggaggcag aggaagtagt tatccctgca tttaatgagt tggatgctta cgtggtcgca
    27961 ctcggagacg aaacaaatat cgaagcattg aaagtggtcc aagcgattcg aaactttggt
    28021 ttttcagctg accgcgactt catgaaccgt aaagcaaaag cgcaatttaa gacagcagat
    28081 aaagcgaatg ccaaattagt attggtgatc ggtgaagatg agcttgctag tcaaacgatt
    28141 aatgtcaaag caatggcaag ccgaaaagaa aaaagttttc cattagaaga gatatatgaa
    28201 cattttgatg aagtctatga tgaaatgact ttattcgagt aggaggaaaa aaataaatgg
    28261 cacaaagaac gatctattgc ggattagttt ccgaacaaca attagaacaa acagttacat
    28321 taaaaggatg ggtacaaaaa agacgagacc ttgggggagt aatctttatc gacctacgtg
    28381 atcgtgaagg aatcgttcaa atcgtcttta atcctgaaat caacaaagaa gcatgggaaa
    28441 tcgcggataa atgtcgcagt gaatacgtga tcgaagtgac tggggaagtt agaagacgcg
    28501 gagaaaatgc agtaaatcct aaaatggcca ctggtgaatt cgaagtgatt gcaagtgaaa
    28561 tcgtgatttt gaatcaagca aaaaccccgc catttcaaat tgaagatgat caagcaatca
    28621 gtgacgaaat acgtttgaaa tatcgttatt tggatctgcg tcgtccaaaa atgactaaaa
    28681 acatcaaatt aagaaatgcg acaacaaaag cgattcgtca ttatttagat gataatgact
    28741 ttctcgatat cgaaacacct tacctaggga aatctacacc agaaggcgca agagactacc
    28801 tagttccttc acgtgtccat gctggacatt tctatgcgtt gccgcaatct cctcaattgt
    28861 ttaaacaatt gctgatgaat gctggattcg accgttatta tcaaatcgtt cgttgtttcc
    28921 gcgatgaaga tttacgtgga gaccgtcagc ctgaatttac gcaagtagat attgaaacaa
    28981 cgttcttaac agcagaagaa atccaaactt acacagaggg attgattgcg aaggtaatga
    29041 aagaagttcg cggtatcgaa gtcacattgc catttcctcg tatgacctat gatgaagcaa
    29101 tggctcgcta cggcagcgac aaaccggata cacgttttga tatggaattg atcgatctaa
    29161 gcgatacagt caaagaagta gaattcaaag tcttccaaat ggctcttgaa aacggcggtg
    29221 tcgtgaaagc attgaatgct aaaggcgctg cagatcgcta ctcacgaaaa gacatggatc
    29281 agttaggtca atatgtagga caattcggag ctaaggggct tgcatggctg aaagtagaag
    29341 aagacggatt gaaaggtcca attgctaaat tcatgggaga agcaacagaa gcaatcatta
    29401 aagcaacaga tgcaaaacca ggtgacttgc tgatgtttgg cgcagataaa agtgaaatcg
    29461 tcgctgctgc attaggtgct atccggacac gtttaggaaa agaattagga ctaattgatg
    29521 aatcgaaatt caatttctta tgggtaactg attggccaca atttgaattt tcagaagaag
    29581 aaggtcgata tgtctctgct caccatccat tcacgatgcc aaaagaagaa gatatcccgt
    29641 tattggctga agaccctgca aaagtctatg cagaagctta tgatatcgtc ttgaacggtt
    29701 atgaattagg cggaggttct atccgtattc atactcgtga gcttcaagaa aaagtactag
    29761 aaactctagg ctttacaaaa gaacaaatgg aagaacaatt tggtttcttg ttaacagctt
    29821 tagattacgg attccctcca catggcggta ttgcattagg attggaccgt tttgcaatgt
    29881 tattagccgg agaagataat attcgtgaag tcattgcctt tcctaaaaac ggaaaagcag
    29941 cagatgtgat gagtgatgca ccaagtatgg tttcgccttt acaattgttc gaattgaata
    30001 ttgatgtaac gaatatagaa gaatagccga taaaaaggta aaaaggattt gtcgaatacg
    30061 cttcttttga caaccttgat ttaattgaaa aacactttat aatgaagcca taacatataa
    30121 agcggggctt ttccatgaaa aaaacattcg aaaattgggc ttatcatgat tacacgacca
    30181 agctttcggt atctatcgtt tatgcgttga tcgcatcagt cgcattgaat ttcttttatc
    30241 agccaggaca tatttattca agtggtatta ctggtctagc tcagattttg acgactttgt
    30301 caaaatcact gataggattc gaagtaccgg tatcaacaac tttgtattta ctgaatatac
    30361 ctttgtttgt attggcgtgg ttaaaaatcg ggaagaagtt tacaattttc acttttatca
    30421 cagtcacttt gacctccctt tgtatccata ttttaccgga aacagtccta tctaccgatc
    30481 caatcatctg tgcgttgttt gggggggctg tgatgggact aggtattggg ctagcgttga
    30541 gaaacggcct atcatcgggc ggtctagata tcgtgagtat cacgattcgt aaaaaaacag
    30601 gaaagtcgat tggttctatt tcgatctact tcaatgcatt gatcgttttt gttgccggaa
    30661 ttctatttgg ctggcaatat atgttttaca gtacgttgtc tattttcgtc agtggaaaag
    30721 taactgatgt cgtttatact aaacaaaaga agatgcaggt aatgatcgta acgaaacatc
    30781 cagaaaaagt agtaaatgaa atccaaaact gtatgcgtag agggatcacg atcatcaatg
    30841 aagcagaagg tgcttataag catgataagc agacagtatt gatgacgatc gttacacgat
    30901 acgaactgcc tctacttaga gaagcaatga aagcttctga tccacaagct tttgtaagta
    30961 ttgcagaaaa cgtccagata cttggtcgtt tctatgagga tggcatttag atcgagtttg
    31021 acaaaaattc atgaaatact gctgtagaca gacgttcgtt tgtctgcagt ttttttctac
    31081 catagtagaa tcagcaaaga aaaactgctt ttttatttga aattttctta aagttgaaaa
    31141 aaagcatgtt tattttttta aatttatggg atgatagaaa aaaatagtag agaaaggaca
    31201 cgattctact tgctattttc acttaatacc cgtatattac gaatgaaaaa agtttatgaa
    31261 aagttggaat attgaatgga aaaacaaaaa aggttaaaaa acagccgcta catcactggg
    31321 tttgatgggc tccgttcatt agctgtggtg ggtgttatct tctatcacct tctgccaact
    31381 agtatgaaag gcggctattt gggcgttccg atttttttcg tcgtatctgg ttatttgatc
    31441 acggatctgc tgcggcagga atgggaacaa aatggaaaaa tcaatatttg gcaattttat
    31501 gtcagaagaa tgaagagact ctatccggca cttgttttta tgcttgtgac agcttcggct
    31561 tatatcactt tgtttcagcg agggctgtta aacaatcttc gaggagttgt ggtcagcagc
    31621 ttactgtatg tcaacaactg gtggcaaatc aataacggtt tatcttattt cgatcgattt
    31681 gctaatgagt caccgtttac gcatatctgg tctttagcag tagaaggtca aaactatttg
    31741 gtttggccga tcttgtttgt attgttgatg gtatttgttc gtaaaagaaa atggatcttt
    31801 tatactgtgt taggtacatc cttcctctcc gccatcttga tgatgatctt gtatacacca
    31861 ggaggcgatc caacccgtgt ttattacggt accgacactc gtctgttttc tatatggttg
    31921 ggaagtgcgc ttgcgtttgt ttggccaagt acgcgtttaa agaaaaacat cccgaaacaa
    31981 gcgaaaaata ttctgaatat cgctggagga atctcgcttt tggcactgat tctttgcttc
    32041 ttctttttag atgatcatta caatttcatc tattacggag gaatgctcct agtgagtctg
    32101 ttatgcgtag tgctagttgc agtgacagca caccccggag caagtttgaa tcgatggctg
    32161 acaaatccag tatttacttg gattggcaaa cgaagctatg gcatttatct gtatcagttt
    32221 ccagttatga ttttttatga agcaaaggtg actgatattg ccgatcatgt tttgttgcat
    32281 actttagtgg aaatcacatt gattctaatc atcagtgaac tttcttatcg atatgtggag
    32341 cgtccgctag cacgttttga ttatggtcgt acattccaag tgatgaaagg atggttcagc
    32401 aaaccgatca tcagtaagag caaaccatgg gttgttccag gcacattact tgtagcaatt
    32461 gcactcgttg gtttcgttac ggcaccgaaa aacgctgtaa cagccgatca aaaacaactg
    32521 caggcaaaaa tcgctgaaag taaaaaatta gcagaagaaa ccagaaacaa ttcagaaaac
    32581 accactagtt cggttgacca agcgatcctt gataagtacg agttgtccaa ggcacaaggg
    32641 aataaggcac aaaaattaga actaacggca tttggtgatt cagttatgtt ggatgctgca
    32701 acggatctac aagaacttta tccaaaagta gtggtagatg gagatgtcgg acgacaatta
    32761 tatgccagtg taccgtacat tgaaaaatta aaggaacaag atttgttgaa agacactgtt
    32821 ctgattggac taggaacgaa tggctcattc tcggaagctc agtttgatag tgtgatggat
    32881 gcaatcggtg atcgaaaagt atactgggtc aatgtccgag taccgacgaa gcgctggcaa
    32941 aatgacgtga atgcgatgct tgaacagatg acgaaaaaat acgacaatat gacgctgatt
    33001 gattggtaca gctacagtaa tgaccatagt gactggtttt atgatgatca agtccatcca
    33061 aatcctgaag ggatgacaca ttacattcac ttagtcagcg aagccttgct aggaggaagt
    33121 tcaagtacga acagcagtga gacaaacgtg acagaatgat agctacaaaa aacttcaggc
    33181 aaaaacattt gtttttgcct gaagtttttt tatatttacc gtgtttttaa cattcatata
    33241 gattcaggaa gcaaaacggt tgagtgtgtc tgtaaataac gttctttttg ttttcgggtg
    33301 agttttttga atgcagcttc tgcttttgtt gcttcgcttc ttgaagaaaa tacttctgca
    33361 tggatcatct taacaggtct tcttttttga agacgagtat atttagcacc agtaccattg
    33421 ttatgttcct ctaatcgtct agaaagatcg gttgtatatc caccataaaa tgtctgatct
    33481 ttacagaata atacgtaaaa ataatgttca tttgccatag agcatctcac gaatctctgc
    33541 agtgtattct ccatccatat tgtaagtgat caatggtgga gcgactttga atccgtctaa
    33601 tttgccttct ttgattcctt caactaacaa aatatttgcc tcttttccag gttttgggta
    33661 aacaaattgg atccttttag gtgcaattcg atagtcttcc atgacctgaa tgatttctaa
    33721 gaaccgatca ggacgatgga ccatagtaaa gcgtccattt gtttttagta gcttacttga
    33781 aatatcgatc acttcttcta acgtcgtatg aatctcatga cgggcgattg ccaggtgttt
    33841 attagggttc gttttatttg ttggcagccc tttgaaataa ggtggattac agacgactaa
    33901 gtcacaagaa ttatgtcgaa cgacagataa gctgtctttc agatcgatgg tgtggacagt
    33961 catttgttca tttaaatggt taagcgcgat actacgtctt gccatgtctg ctagacgagg
    34021 ttgaagttcg atagcgtcga tagccgcctg ggttcgttta cttaaaaaaa gtccaactgc
    34081 gccatttcct gcacaaaggt caacaatctt ccctttttta ggtacacggg aaaagtgagc
    34141 aagcagcaca gcatctaaag aaaaagaaaa cactttggaa ctttggataa tcttgatatc
    34201 ttctgcgtat aattggtcga ttcgttcgtc ttcgtgaagc atggattccc tccaaaatga
    34261 aaaaaatagt cgtctgtatt atactatttt tattaaaaag aaccaaggtg gcttgtcaaa
    34321 cctctatttt ttatgcatac tatgaccata agaagggaaa ggaagagact atgttttatc
    34381 gatttatgcg cggggtagtt cgagtactat tagccttagt taatggaaat gcccgctatg
    34441 agaataaaga agttttaccg caagatgaga attacatact agttgctccc catcgtactt
    34501 ggtgggaccc attatatctg gcggtagctg cagcacccaa ggaattcagc tttatggcaa
    34561 aagaagaact gttcaaaaat ccagttctgc gatttatatt aactcgatcc aatgcttttc
    34621 cagttaaaag agaaaatcca ggaccaagcg tcattaaaac acctgtaaag attttgaaat
    34681 cgacaaattt gagtttgatc atgtttccca gtgggacaag acatgctaca gaacttaaag
    34741 gcggaatggc attgatttcc agaatgtcta aagcgaaaat cgtgccagct gtatatcaag
    34801 gtccgttgac acttggggat ctgttcaagc gtcagagagt gacagtgcgt ttcggcgaac
    34861 caatcgactt atcagatatc aagaaaacgg ataaagaagg tatggcggag atcgaacgac
    34921 gaacacaagc agcttttgac aaattggaca aagaagtgaa ccctgatttc aaatatgaag
    34981 tcaaaaaaaa ataaaatgat aagtttatgc gtgggcggtc ttttgaatct actgtcctgt
    35041 tcagttcaga ctttttcaat gaaaacgagg gagagacgtc aagtctgccc tctttttgcg
    35101 ttccaatatg taaaatagaa gacaggtgtc atgtaatgga tcaaagtgtt ataataatta
    35161 gaaggacatt ttttgaggtg attgaatgcg ttttttacat acagccgatt ggcatatcgg
    35221 aaaaaaacta caaggttatg acttgattga agaccagcgt catgtactat cttctatact
    35281 agatatcgcg aaagaagaaa aagtagaggc aatcgtgatt gccggagact tatatgatag
    35341 gagtgtccca gctgttgatg ccgtagaatt atacaatgaa ttgatggtcg attggaattt
    35401 aaaggaaaag atcccgattt ttgctatatc aggaaatcac gatagcagta cgagactttc
    35461 agcgggtacg ccatggttca gttattcgaa ctattttttg cgaacacagc tagcagaagc
    35521 atttgaacca gtcgagttta aaaacgtaca atttttcttt cttccttact ttgaaccaat
    35581 tgcagcgaga ttatattttg aagatgacaa tatccgaacg atcaagcaag cggtagccaa
    35641 ggtgatagat aaaatgcagg agctatttga ttcagaaaaa aaacaagtat tgattagcca
    35701 tttttttgta atgggaagtg taaaatctga ttcggaaacg aaaatcgaag taggcgggtt
    35761 ggattcgatc cctggtgaac tacttacacc gtttgattat gtagcactag gacatttgca
    35821 taatcaagct gctttgagac aggataatgc tcgctatagc ggttccccat tgaaattctc
    35881 tttatctgag atcaaccaaa aaaaaggcgt atggatcgtt gacctgacaa caggcctgga
    35941 gttgaatttt agggagatcg aacccctgca tgatattgaa gaaataacag cgagtttcaa
    36001 agaattgctg gatcctgcct tttatcagtc cgtcaatcgg gagaattaca tctatatcta
    36061 tctcactgat agaacgatca tcccgaatat gatgaaccag cttcggcaaa tatatcctcg
    36121 tattttgggg attgaacggg caaacggtcg agcagaacaa acaaagcgca aacgaaaagt
    36181 actagaaatg aaagatcctc aaaaattagc agaagcattt tttagtgaaa tgacggaaga
    36241 gcagctaact gcaaaccaag aaaaatggct cggagaaacg ttgcaagaaa tcatggaaat
    36301 ggaatgatca agaatgaaac cgaaaaaatt acaacttaaa aattttggtc cttttatcaa
    36361 tgagaccgta gatttttcac gtttgacaga agcaccgctt tttttgatca gcgggaagac
    36421 aggagctggc aaaacgacga tttttgatgg gatcaccttc gcactttttg gtgaaacttc
    36481 cggaagactt cgctctggaa aagagatgcg ttctttgttt gctacaccag acgaagaaac
    36541 aagcgtgact ttttcttttg aacatcagca gatgagctat gaaatcgaac gaaaaccaga
    36601 acaagtttta gcaaaacgaa aaggaaacgg aacaaaaaaa caggcagcca aagtaacgtt
    36661 gaccattttt gatgggaaag gaaaagaaat aaaacaaatc acaaaacgta cagaagcaga
    36721 tcagttgatc agagaactga tgaatctcga tgcgaaacag ttttctcaaa tcgttttact
    36781 accccaagga gaattccgta attttttggt ttcatcaagc agtgataaag agactgtttt
    36841 acgtcattta ttcggtactc aatttttcca acagttcaat gagcgattaa aagaaaaagc
    36901 aaaacaacag caacaaaaat tggatcattt agagcaagag ctgcatcttc tgcaaaaaag
    36961 gtttgtttta gtcaaagaag aggaaccagc agcagggttt gaagccgttt tagcccaatg
    37021 gacaaaccat caggcaatac tcgaaaaaca aatcacggaa gaaaaagaag ctctaacgga
    37081 acttcaggaa aaacaaaagc aactagaaaa ttcctactat cgttttgaaa gcttgaaaaa
    37141 aagttatgaa gaaaaaacga tattaataaa caagcaaaag gaattagctg ctcaaaaaga
    37201 cgaaatagag gaaaagaaac gatggattgg gtattatgaa tttacgcaac gattagtcga
    37261 accaatccag cgcctacaag aaataaaagt tgaaaaaata gaactgtcac aaaaaagtag
    37321 tgagcaaaca aagaaactag cgatgactac gcagaattat gaagagtggc aaaaaaatga
    37381 agcaaaccgc gtacgccgac aaacaaaaat caaagaagct gctcaaaaac tgcaaaacaa
    37441 acagatgatg gtgccaattg tcgaatcctt acgtagtaaa aaacaagacg cagaacaatt
    37501 aacaatttca ttggataaag aagaattgaa tgccgagaag ctgtatcaaa atctcgctat
    37561 ctatcaggaa agaactcgaa ttcttcaaga aaatttactg gatcaggaaa aactgcaaga
    37621 agatcgtctt gcttttggac agttaaaata tctagatgaa aagcagcaag aaatagaaaa
    37681 acaaaaagca aaagacactg ggtcgctcgc acagataaga gaagaactca aaaaagcaac
    37741 tgaagaaata acaaaaaata gtcagctatt gaaagaacag acagaacaag cagaacagct
    37801 gaaaagccaa tgggcaaagc agcaaattgc tcgtcttcag ctttttttgc gtccagggga
    37861 gccatgtctt gtttgtggat caacggaaca ccccatacaa caattaaaac acgaacagcc
    37921 aaataaggaa gatatcttaa aagtagaaca acaactggca gaagctgagt ctgctgtaga
    37981 aagaactcag caaatcattg ccaaaagtga agcacatgaa aataatcttc aacaacaatt
    38041 atcagattta gaacaaaaaa tcatggcaac gaatatacaa ttggctgaac aagaagaaca
    38101 cttgcaacag gctttgaaaa aggcatttcc tgagataaca gaacaaaaaa caaaaacaac
    38161 tcttgtagta atcgaacaat ctttagatag acgaaaaagc aagatggcca atgaaagaat
    38221 agagctggaa gaaataagac aacaagcaga aaagctcgaa caacaattag ctgtctacaa
    38281 acagcagcag acagaaaaaa ataatcagct ccagcaactg caaggagaaa tcaaaagttt
    38341 agaacaacaa ttgcaagata cggatagtaa ctgtttagaa gaggagatac gacgacttga
    38401 aaaacagtta gaagaagact cgaatcaact agctgttgag cagaaagcag gagtgcaatt
    38461 gttgcaagat cttcatacgc ttcggacaca gcttgatctt caacatgaac aaatagaaaa
    38521 aatagatgaa aaaatagaaa aggaacaaga aagaatagct aaaaagttag aaagccagtc
    38581 gtatttttca acaattgaag aaatctgtgc atttgcacaa cagcaagcac aatataaaga
    38641 aaaccaacgt acagttactt cttatcatga agagcaatta gtgacagctg accggctgcg
    38701 tcaattagaa aatatccagc taactgagac attcccagat acagaagaga tcagccaaca
    38761 gttagaagaa ataagacgta tgatcgaaat aaagcaagaa aagatatacg gtttacaaga
    38821 acaaaaaatt agtaatcaaa aaatctacga tgaatgtctt tcgatttatc acacaagcca
    38881 gacgaaatta gatgagctta cgcaattaca gcagctttcg caaacattca acggagaaaa
    38941 tcctaagaag actagtttgg aacggtatgt gcttcaggtt tatcttcaag aagtgttaca
    39001 agtagcaaat gatcatctcc aacgtttaac gaaaaatcgt tatcaatttg agctagcgga
    39061 taccattggc agttatcgag ggaaaaccgg attagaaatc aatatctatg atgatcaggc
    39121 aggtatgtct cgaagtgcgc ataccctgtc tggcggtgag agttttattg cagcactttc
    39181 tttagcatta gctcttgccg atgtgatcca gacacaagct ggtggtgtag caatcgatgc
    39241 attgtttatt gatgaaggat ttgggtcatt agatgaagaa gctttggaaa tggcaatgga
    39301 agctttagaa acgatcgaaa gtgaaggaag aatgatcggc atcattagtc atgtaagaga
    39361 attgaaagaa cgagtattac aacaaataag aattgataca gaaggtagcg gacaaagcaa
    39421 aatacgctat caacttggat aaaaagggag attggtaact atgaaatgga gtattccaga
    39481 aaaagtcatt gcacgtggac gtcaatactt gcaagaaggc cgagtgttat cggttgtccc
    39541 tgaccaagaa gaaaacgtgt ggcatgcaga agtcttgggc agtgaattgt atatggttga
    39601 tttggatggt acagcaaaag agatagatta ctgccaatgt ccctattggg aggaacataa
    39661 atattgtaag catacagttg ctgtggaact ttatttacgg tcgaaaaatc tatctcgtat
    39721 catgaaaaaa gaccagccaa tgctagaacc ggtaaaagca acaagtgagg gagaaatcat
    39781 tgcaaaaggt ttttctcgtc taaaaacaca aaacaatcct caagcggtgc aacctttgat
    39841 tattgaatac caagtagaaa cgattgagac taatcagtat cacccagagc tttctatttt
    39901 ggcactttac ttgcgtatcg gttcattggc atcaccgaaa aaaacatata tcgtaaaaaa
    39961 catctatgat tttttacaag cttacacaga aaaagaaact tacactgtta ataaacagta
    40021 ccagtttcgt ctagataaac gggcgtttca aaaagaagat caaaaaatac tcacgcatct
    40081 tgcaggaagt gctcagacgc atcaattatt aggtacgaac ggcttacaag tcaaaggaaa
    40141 actagataaa agatatctgc ttctcccagt agaacaagcc cgcttcctgt tagagcagat
    40201 gaatcagact acacgtttgc atctgcagat aaatgaacaa aacgatcaag gaatacaatt
    40261 ttctgatcaa aacaaacctt tatcattcaa agtggaaaaa tttggagaag attatttgct
    40321 gcaagcattg aatgattttg attttttctt tatgcattat cagtggggag ttatccaagg
    40381 gacattttat tctctgacga aatatcaaca aacggtttat caaacttgga aacaattgat
    40441 tcgccgattg gagaaaccag aagtagtgtt caagaaaaaa gaattaccgt ctttgtttaa
    40501 agagattatc cctctgctgt cagagatcgg cgacgtagct attgcccctg cagtagaaga
    40561 ggaagtagca gatatccccg tcgaatttat tttctttttc cgaaaagcaa aaggaaaaat
    40621 ccaagctcga gtcgatttcg tctatggtga agtcatctat tcgactgacc cgaagcatga
    40681 ggtctcgaca gaacaggctt ttcaggtgtt acgtgacaca gtagaagaga aacgagtact
    40741 tgatctattc agaatgtatc gctaccagaa aaatgaaact ggttatgaac gtccattgcc
    40801 aagcggggaa gaactatatg cctttttccg cacagaacta tctgtcttcc gtcagtttgg
    40861 tgaagtcaga cttggaagaa aattgcgaga gttgtattta gatgcgcata aacatcaacc
    40921 gaaaatcgaa gtaaccgaga ccggttcatg gctcgatatt cgattcgatg tgacagggat
    40981 agaggagcaa gaaatcgatc atgtcctgca aagcttgctg agaaatgatg ctttttatac
    41041 attagaaaat ggtcaggttc tttcttttga ttcagaagaa tttcaacaaa caagccaagt
    41101 gttgcaacaa ttacgggaat cgatacgtac agaagaagga acgatccatg tacctaaaaa
    41161 tcaaggattg atcattcaaa atcaattgga aaaaagcaat gctactttta gtgaatcgtt
    41221 ccaaacgatg gtacaagatc tcattcatcc agaaagatac caggcacagc ttcccaaagg
    41281 attgaatgca acgatgagag attatcaaaa gcaaggtttt cgatggttga agatgttagg
    41341 tcattatcag tttggcggta ttttagccga tgaaatggga cttgggaaaa cactacagac
    41401 aattgccttt ttactttcgg aaaaagaaga acggaaaagc ttctctgctt tgatcgtagc
    41461 gcctgcgagc ttaatttaca actggcaggc agaggtgaga aaattcgccc cttctttaag
    41521 tatccaagta atcaatggga ataaaaaaga acgtgaagaa ttactggcta aagacacaga
    41581 tatccgagtg acttcttatg ccagcttaag acaggatcta gctaattatc agtcgcaaaa
    41641 aattgattat ttgattcttg atgaggcaca aatggtcaaa aatagcagta cgaaaactgc
    41701 acaagcattg agagaattag ctgtacctca gcggtttgca ctgagtggga caccaatcga
    41761 gaataattta gaagaattgt ggtcattgtt tgcaacgatt atgccaggat ttttcccgac
    41821 taaaacaaaa ttcagggaac ttgccgcaga agaaattgcg caaatgattc gaccgtttat
    41881 tttacggcga gataaacaaa ctgtgttgaa agacttgccg gaaaaaacag agatgaatct
    41941 atattctgca cttacagaag aacaaaaaac tgtttattta gcttacttgc gtcaaatgcg
    42001 tgaagaaatc acatcaatgg attctgaagc attcaagaaa aaccggatcg ggatacttgc
    42061 tggtctgaca agactgcgac aaatttgctg cgatcctcgt ctgtttattg aagattacca
    42121 aggcggttcc ggcaagctcg aacaagtaaa agatctactg gtagctgcca aagagaacaa
    42181 acgaagagtt ttgctgtttt ctcaatttac agggatgctg actatcttgc aagaagagtt
    42241 agccgagcta gggatctcga cattttattt aagaggcagt acgaaaccac aagatcggtt
    42301 aagtatggta gacgcattta atgctggcga aaaagatgtt tttctgattt ccttaaaggc
    42361 tgggggaact ggattgaatc tcacgggagc agatacggtc atcttgtatg acctttggtg
    42421 gaatcctgcc attgaagaac aagctgctgg acgtgcacat cggatcggac aaaaaaatgt
    42481 ggtagaagtg tggcgtatga tctctgaagg aacgattgag gaacgcatgg attcactcca
    42541 gcaagaaaaa cgcgaactgt tccaaaaagt gatccaaggt aatgaagaac agttgacgaa
    42601 actgactgaa gaagacatcc gcatgatttt aagtgtagga gaaatcgagg agtaagagac
    42661 tcgaaaaagc gttcagtaat gggtaagaaa gcctttctaa agaagcgatt gtttatttga
    42721 tttaaaaaat agcaaggagg ctgggattcc attttatccc agcttccttg ctatcatttt
    42781 tttatttcta tttttaaagg ttatacaaag attgtcttca ttgtctctaa cagctgatca
    42841 aaagaagaaa tctcgatatc gcaaattgga gtctgttcaa ttttgcgttc tcgatggttg
    42901 aaccaaagcg cttgccagtt agcacttttt gcacctagaa catcattgtc gtaattatcg
    42961 ccaacgtata aggtctcttc tggaagcata tgaaattctt tctcagccag ctgaaaaatc
    43021 tctttttctg gtttttggaa acctgttgct tgagaaatga gcatattgcg ggaaggaatc
    43081 caattgttca attgaagttg cttcagtttt tttgtctgat ggtcagtcgg cccatttgtg
    43141 ataattccta atgggacatt cttttccttc aagaaatcga gggttttttt gacctcctca
    43201 tgcaagcaga tattatcaag ttcttcctcg taaatttttt gaaataagag gccatcttct
    43261 tccgtaatat gagggtagtc taaatctttt agtgattggc tgatccgatg tgctcgcata
    43321 tattctagtg tccaatctcc tgccattact tttgggaagt tttcatcact atgatggcgg
    43381 aaccggatat atagtggatg catatctgca tctgaaacaa gcggaacgac tcttttgacc
    43441 gcgtttcgaa atggctgctg ctgatcatac atcgtatcat caacatcaaa cacaactgat
    43501 ttaatcaaaa ttttacacgc ctttcggttt tcattgattt tttcacaaat ccttgactat
    43561 tgtaacagaa aaaattgtct aaaaaagctg ataaataaag gtttttctta ctatatttct
    43621 atttccttga cagaataagg tttttatatt tcagtttttt tcactaaaaa tcgtgaaaca
    43681 cgtcaataag tttgatgaac aaattaatca ttatggtaga agtcgattga tttattttaa
    43741 tgcaatctct gtggaatttt cgaaagattt atttttttaa ta
//
LOCUS       DARDHA010000010        40933 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000010 DARDHA010000000
VERSION     DARDHA010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.010"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(37..1188)
                     /locus_tag="SDA10_000436"
     CDS             complement(37..1188)
                     /locus_tag="SDA10_000436"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEG4239367.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1201..1638)
                     /locus_tag="SDA10_000437"
     CDS             complement(1201..1638)
                     /locus_tag="SDA10_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4239368.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1653..3419)
                     /locus_tag="SDA10_000438"
     CDS             complement(1653..3419)
                     /locus_tag="SDA10_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEG4239369.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3683..4360
                     /locus_tag="SDA10_000439"
     CDS             3683..4360
                     /locus_tag="SDA10_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4239370.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4363..5490
                     /locus_tag="SDA10_000440"
     CDS             4363..5490
                     /locus_tag="SDA10_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4239371.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5690..6130)
                     /locus_tag="SDA10_000441"
     CDS             complement(5690..6130)
                     /locus_tag="SDA10_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4239372.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6141..8174)
                     /locus_tag="SDA10_000442"
     CDS             complement(6141..8174)
                     /locus_tag="SDA10_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG4239373.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8176..9879)
                     /locus_tag="SDA10_000443"
     CDS             complement(8176..9879)
                     /locus_tag="SDA10_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4239374.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9973..10923)
                     /gene="manA"
                     /locus_tag="SDA10_000444"
     CDS             complement(9973..10923)
                     /gene="manA"
                     /locus_tag="SDA10_000444"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEG4239375.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10962..11987)
                     /locus_tag="SDA10_000445"
     CDS             complement(10962..11987)
                     /locus_tag="SDA10_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEG4239376.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12196..13065)
                     /locus_tag="SDA10_000446"
     CDS             complement(12196..13065)
                     /locus_tag="SDA10_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HEG4239377.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13524..15416)
                     /locus_tag="SDA10_000447"
     CDS             complement(13524..15416)
                     /locus_tag="SDA10_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4239378.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15413..17140)
                     /locus_tag="SDA10_000448"
     CDS             complement(15413..17140)
                     /locus_tag="SDA10_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4239379.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17156..17806)
                     /locus_tag="SDA10_000449"
     CDS             complement(17156..17806)
                     /locus_tag="SDA10_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEG4239380.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18010..18306
                     /locus_tag="SDA10_000450"
     CDS             18010..18306
                     /locus_tag="SDA10_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG4239381.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18478..19098
                     /locus_tag="SDA10_000451"
     CDS             18478..19098
                     /locus_tag="SDA10_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEG4239382.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19190..20356)
                     /locus_tag="SDA10_000452"
     CDS             complement(19190..20356)
                     /locus_tag="SDA10_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG4239383.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20368..20721)
                     /locus_tag="SDA10_000453"
     CDS             complement(20368..20721)
                     /locus_tag="SDA10_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEG4239384.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20849..21871
                     /locus_tag="SDA10_000454"
     CDS             20849..21871
                     /locus_tag="SDA10_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEG4239385.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21890..22051
                     /locus_tag="SDA10_000455"
     CDS             21890..22051
                     /locus_tag="SDA10_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239386.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22310..22744)
                     /locus_tag="SDA10_000456"
     CDS             complement(22310..22744)
                     /locus_tag="SDA10_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239387.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22854..24713)
                     /locus_tag="SDA10_000457"
     CDS             complement(22854..24713)
                     /locus_tag="SDA10_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG4239388.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24831..25991)
                     /locus_tag="SDA10_000458"
     CDS             complement(24831..25991)
                     /locus_tag="SDA10_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HEG4239389.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26298..27722)
                     /gene="araA"
                     /locus_tag="SDA10_000459"
     CDS             complement(26298..27722)
                     /gene="araA"
                     /locus_tag="SDA10_000459"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEG4239390.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27738..28436)
                     /locus_tag="SDA10_000460"
     CDS             complement(27738..28436)
                     /locus_tag="SDA10_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEG4239391.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28393..30054)
                     /locus_tag="SDA10_000461"
     CDS             complement(28393..30054)
                     /locus_tag="SDA10_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEG4239392.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30272..31672
                     /locus_tag="SDA10_000462"
     CDS             30272..31672
                     /locus_tag="SDA10_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEG4239393.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31924..33003)
                     /locus_tag="SDA10_000463"
     CDS             complement(31924..33003)
                     /locus_tag="SDA10_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4239394.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33089..34048)
                     /locus_tag="SDA10_000464"
     CDS             complement(33089..34048)
                     /locus_tag="SDA10_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEG4239395.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34060..35571)
                     /locus_tag="SDA10_000465"
     CDS             complement(34060..35571)
                     /locus_tag="SDA10_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEG4239396.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35593..36156)
                     /locus_tag="SDA10_000466"
     CDS             complement(35593..36156)
                     /locus_tag="SDA10_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEG4239397.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36162..37025)
                     /locus_tag="SDA10_000467"
     CDS             complement(36162..37025)
                     /locus_tag="SDA10_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4239398.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37039..37935)
                     /locus_tag="SDA10_000468"
     CDS             complement(37039..37935)
                     /locus_tag="SDA10_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4239399.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38110..39399)
                     /locus_tag="SDA10_000469"
     CDS             complement(38110..39399)
                     /locus_tag="SDA10_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4239400.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39371..40888)
                     /locus_tag="SDA10_000470"
     CDS             complement(39371..40888)
                     /locus_tag="SDA10_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEG4239401.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
BASE COUNT        11795 a         8582 c         7091 g        13465 t
ORIGIN      
        1 aaaatttcgt cacattcccc tttttcgtat cctattttaa gcgacttgct tggcataacg
       61 gttcacgttt tgcttgatat tcccgattgt tttcggatca ttgattcctg tgacttcttg
      121 aatcaattgt tccaagtttt catggatctt catttcatgc aattgacggc tttgttcatc
      181 ttcaggatca tagtaattaa aaataatccc cattgttgcc gtcaaatgtg ggcaagtcaa
      241 gttatgttct tgtagttctc tcactggacg tgtaaatctt tcttgataac ctaacttacg
      301 taatggtgtc cgagcgacac gtgtgatcgc atcagagata tttttgtttt ggaagcgatg
      361 aatgattttt tcaatatacg catgatgctg ctctgcatca aacccccatt tggcaatcag
      421 caaacttcct gtttcctgca aaactgattt taattgtgcc acaactaacg catcttgcat
      481 tgcttcatca atggtctggt aacctaacaa ggcacctgta taagcgacag tcgcatgacc
      541 agtattgaca ctaaatagtt tgcgttcgat atatggttct agatcatcta catataaaac
      601 cccttttaat tggatttttt tattttttct ttggctatcg tcaatgaccc attctttgaa
      661 aggttccact tggacgaata aaggatcttc atggtgttgt aatgggacga ttcgatcgac
      721 tgctgcattt ggaaagccga tatatttgtc agtaaatgat gtttccggaa gatatttata
      781 cacttctttt tctagaaatg ctgaaccgcc aatcatattt tcacaggcaa tgatatctaa
      841 agatgttgtg cttccttcct tttctctttc ttggatacct tttgcaatca actcagcaat
      901 aaaaggtaag atattcgggc caatggcggt agtaactaaa tctgcttcag cgatttcttg
      961 gattactttt tctggatttg tttgattgtt gattcctttg acatttttta cagtgatttt
     1021 ttcttttgat tcatctgcta attcgatttc gtattctttt ctttcattta aagcatcaat
     1081 gatcgtgtga ttcacatcta cgaaagtaat ctcaaatcca ttagctgcca aaacctctcc
     1141 aataaatcct cgaccgatat ttcctgcacc aaaatgtact gctctcattt ttctctctcc
     1201 ttaagcaatt gctagatttt ccgttacttc ttctttgctg agcgcgtctg ctagctggac
     1261 aacattgtcc atatcactgc aataaagtgc gatttgctgt acaagttgca aatgctcatc
     1321 tccgactcct gcaataccga acaaaacagt cgcaatcttt tcttcttctt cggtaccaaa
     1381 atctacacca tctggtactt gaacgacaca gatgccggat tttttcacat atttttttgc
     1441 ttcatctgtt ccatgaggaa tcgcgataaa attccccata taaacagata acattttgtc
     1501 tctatctacc atggcatcga tatattcctc atcaacgcac cctgcttcta caagcatctg
     1561 accgcaatag cggatcgctt cttctttggt tttaaaagat cgattgagct cgatcatgtc
     1621 gaatggcaaa ctttgcatgc tgttttcctc ccttaagctg tctgtttcaa atattgtgca
     1681 atcgattcgt atttttgcgg attgaccatg ctgttgactg ataaatgaat ggattgaggt
     1741 gcagccatct gtactttttc cgctaaactt tctttggcta cgatcagtga atcaggttca
     1801 tcttttaaat cttggatatt gacttttctt aacgggatag ttaaatggtt cttgtctaat
     1861 atatctttga gcacggtcat ccccattgtt gctgatccgg aacgatcatc gtggacaagt
     1921 agaatctctt tgatttgttt gatgtttgct gaaagaaact cttctgctgg ctcattctca
     1981 atttcagaaa ctgatgtttt ctgcttgctg cttaattctt gaatgatttc atcatatcga
     2041 ggtgaattca aaaagttttc tactgagaca aaacgcgcgc tcggtgcttt ttgttttgct
     2101 ctttcttgta gttcgacctg agtaacgatc aatgtattcg agtcatctgt cagttggctg
     2161 attgcttgat tggtcaccgt ttgaggcaag tctgcttcct gtactttttt tctcaagatc
     2221 gaagcgccca tcgcacttga tcccattcct gcatcacaag caaaaatgat tttttgtacg
     2281 ccagagaatg tagctccttg aggaatacct tgggcacctt tggaagcttg cttagctgcc
     2341 tttgtctctt gaacagattt ttcgaagtct tcatttgtat ctttcgcatc cgctttcaaa
     2401 attactgcag aaataccaaa cgaaacagct gcggcaacaa tcacgcccaa aataacagga
     2461 aggtaagaat tgaacggtgt catcgctaga atcgcaatga tcgaccctgg agaagctggc
     2521 gctcttagtc cagcatccat caattggaaa atgaaacttc ctgacattcc tccgcctata
     2581 actgataaga ataataacgg tttcatcata acgtaaggaa aatagatttc atggatcccg
     2641 cctaaaaagt gaatgatcat ggctcctggt gctgaagatt ttgctgctcc tttgccaaac
     2701 aaagtaaaag caagtaatac gccaagtcct ggaccaggat ttgcttcaag caagaataga
     2761 atagattttc cagcctctgc tacttgttca gttcccaatg gtgtcaaaat cccatgattg
     2821 attgcattat tcaaaaataa gatttttgct ggttcaataa aaatacttgt caatggaatc
     2881 aaatgggcag ataaaatgat ctctacacct tttgccattg cttgagtcaa tgcatcgaca
     2941 actggaccaa tcgcagaaaa acctaataac gctaatccaa aaccgataag acctgctgaa
     3001 aaattattga ctagcatttc aaaaccggat ttgatccgtt tttggaatag ctgatcaaat
     3061 ttcttgatgg catatccacc aagaggtccc atgatcatcg cgcctagcat cattggaata
     3121 tctgttccga caatcacacc catcgttgca attgccccaa caactgatcc gcggtcacca
     3181 gcaattactt ttccgccagt ataaccgatc aataaaggaa tcagataagt tagcattggg
     3241 cttaccatgg tagccaaagc ctcatttggt aaataaccat ctgggataaa tagagccgtt
     3301 aatacacccc aggcaatcag tgctccaata tttggcatga ccatgcttga taaagtactg
     3361 cctaattttt gtaccctcgc ttttagcgaa atgttgtttt tgggaatcgt ccgttccatt
     3421 ttgtctctcc ctctctcctt ttgatactct aattgtatcc gcttactaaa aagagaaata
     3481 gtcgtacttt ggcacgaaac tttgtgccaa aggtttgagt atgtcagcaa aataaaaaga
     3541 tttcggtatc agaaaacttc aattttaaat aaaactgtta ttttaatttt tttataatat
     3601 gcaaacaata acttttgtta gcatttaatt tagatgtttt ccattctatt atgttattaa
     3661 attttatatt ggaggcctat ctatgacatt actgatcaaa aacatgaaca tgaaacgtgg
     3721 taaaaaacgt gtgattcgac aattgaatct tttagtagaa tcaggagaag tagctgcttt
     3781 gattgccccg aacggatttg gcaaaacaac tttgctgaat ggcattgccc aactgctgcc
     3841 aacaacttat gatcaattgt cccttcacaa catctctcct actgcttata cagattatcg
     3901 ccggtctttc ttttttctag aatcaacgaa tcagttatcc gactatctat cggctgttga
     3961 ttatttgact ttgatcaaat actattggca gagcaaagta gaatgccaaa ctgttttcca
     4021 acgattgaag atgactgagt accttacttt gccgattgga aaaatgtctc taggaatgaa
     4081 acaacatgtc ttgcttgctg cttacttgat cagtgattca tctgtcatgt tattcgatga
     4141 accgttgaac ggattagacc caggaagtat tgatatcttg aatagcctca tcagacaatg
     4201 gcagaaagag gggaagacga ttcttctttc ttcccacgct atcgcgaata tccaatcgat
     4261 ttgttctaaa gcatgttttc taaaaaatgg agaaatcgtc acagaatcct caaatatgga
     4321 agagatttta gctatttatc gtaaattata tgaggtgtaa ctatgtcttt acttacattt
     4381 gaacgcaatc tcttactaaa aaacaaagta aactttttat ttcctctatt gttggtcgtg
     4441 ctctttgcat tcccactttt ttttgatcat aaacttgctt atacagaatt cgatgaactg
     4501 aatcataact atgaagagat gcaacgtttg attgaaacgt tgaaagaaga tgagaatgag
     4561 aaagaatttg tggaaagttt agagaaaagc aacaaactga tcgaagcaat cttacatgca
     4621 aaaaacacag ggaatgtcca gcaaatggtc gaagcaactt atcattatga aaaagatata
     4681 ctggatcgac tgatttctgg tcaaagacaa ggaattccaa tcattgaaca acaaaaacgt
     4741 gtcgagttgc tccgttatat gaaagaacat cagatccaaa gatattctat ttttgacttg
     4801 ccagcgcatc tccctttagc aaattactac gaaaatattt tttcaggaat gatttctagt
     4861 tttttgattt tatgtataac cgccttgttt ctttcttcca tcatctctta tgaaaaaaga
     4921 aagcaggtaa tctctctagt caatctatta ccggatagta tggtaaagaa acatagcatc
     4981 cgttttacca tttattatgg tgcagctatg ctaagtctcg tgatgccatt tttgatcgtc
     5041 tctatactag taataatcaa aaatggtttg ggagacttcc gttatcctgt aggaacgatc
     5101 attggtcagg agatacgaat ccttccgatg tatgagtatc ttttccagag ctttctgttc
     5161 ctgttgttgt gggtgctctt tttaagtaca atcagttttc ttctatctgc tttatttgaa
     5221 cattctctag tcaatctttt aggtacactt ctttgtcttt tcttagcaga gtatcgcttg
     5281 ttttcttcga ttggatggat agaatcaatc agtcattact tgccaacttc ttatgttgat
     5341 tttcaaaatg tgatcatagg aggagatatt ttctcgccac tagcttcaga acaggttact
     5401 ttcatgaatg gtatcttgac acttggtata tggagtatcg ttttattatt cataggtatg
     5461 ggcactatct atataaaaaa gagctattaa aacacaaaaa taataggggc ggtgacaaaa
     5521 accttgtcac cgtcccctat ttcaaataaa cggttttcta gaataaatag ttttcaaaag
     5581 cttgcatccg cagtaataag aacctcccaa caaaaatagc tcctcttatt tttatcttgg
     5641 ttgaagttat tacttgatgc aaggcagctt tttttactgt gaacattggt tatatatgtc
     5701 catgttcaaa aatcatttgg acttcttcaa tggttttcgc atcactgagc gccattactt
     5761 ggtcgatgtc tgaacagaaa aaggctaaat cttggagcac cgctaactga tgttcttttt
     5821 ttaaagccac tacaaataag agtgtcgcga tttttggttc ctcttttgtt ccaaagtcca
     5881 caccatcagg cacttggact aaatagattc cttcttctac tacttcttca tctttgtcag
     5941 tcccatgtgg caaggctacg aaattcccga tatagacact cgctttttct tgtctttcga
     6001 tcattgattg gatatatgcc ggtttgattc ccccttgttg ggtcatccac tggccgatct
     6061 cggtcaacgc ttctttccaa ttttgatagg attgatcgag caatattttt tctgctggta
     6121 gctgtatcat cttttcacct tcatttcttt atttcttcaa tcaggatttt cgctaacaac
     6181 tgataaacga tttctttatt tcctgagcgg aagatctcgg tgttcaagtc attcatgatg
     6241 attgctccac tgatctttcc taaaagaatc ccgtcttttt cttggattgg cgaaggcgcc
     6301 agcataacta acatacggga caaatcgact ggctgtctat ccatcccttc aatggtcaac
     6361 ggatgtttca aattgaaaat acaaaagatc ggtttagaaa tcaatgagct ggatgtatgg
     6421 aataacccca tatttgtatg aggaataccg ataggtgctt gttcatatcg cttaagcagc
     6481 cggcgatgaa ccacctcttc atccgttact agtccttcag gaaaataagg aaataagctg
     6541 cgaatcgttt cggaaatcgt ctcaggatta tccagttctt ggataaaaaa acgaccgagt
     6601 aattgattga tttgatccat gaaatgcatt gtttcctcat aagtatcttc tatcatccac
     6661 tcgttattgc tgttatacgt aattttttgc ctttttttct tctcaatatg cgaaaaagct
     6721 tcttttagct gcttgacttc atcttctagc agtagcgggg agactaaaat atattttcca
     6781 ccgtatcctg gtaaaataga tgtagaaata acaacatcat attcttcttg taaatcgatt
     6841 ttttttaaat cagcaatccg atagaaatga atttgttgga tgaagggaaa ataccttttc
     6901 aatcgtagtt ccaacatgct agtagaagct aatccacttg gcgaaaatcc tgcaatactg
     6961 atattgcttt ctttcggact tcgttccaat gaattcgcaa aatgaaggac catataagcg
     7021 atctcttctt cggataattt tttttcttga aaaatccgct ctactgcttt acggattgca
     7081 ttcgcaactt cctcgtattg aaccatgatt ttttctaaaa tcggattggt cagtgtctct
     7141 tcttgtaaaa cggctcttga aaacacacca gataaatgag tcaacagcat tttgtacaaa
     7201 gtttcatctt cataaaaatg gacattcgtc tcttcactta cttcttgaat cagttgtttt
     7261 actaaataag caagctgtgt atcgaacgtc tcttcaaaga aattctgatc aaatgagttc
     7321 gtgaagtcat ttaataatcc tgcaaaaaag acgatctcat ttactggata aagttgtttt
     7381 gtctcagaag cgatccgtga aaacaatcgt ttagctgtct gtagtaattc tttgtttgcc
     7441 agactattat atgtctcatt tgtcagtaaa tgatttttag tcacacgctc catcgtcaat
     7501 gtcagcatca aaaccaaatg ttcaagcttt ctatcactta gattggtctt tttttggatc
     7561 aattcttccc cgattacttt gcgtgcaaga gatagatgtt gcggatcaac gaatcgaaaa
     7621 aaataattca gtgatgcatc gaaagaaata ctatgaaaaa gttcatattc attgatttct
     7681 aattctaata cattcgctgt cagaagccgg cggtattttt ccggtccttc gatctcgtat
     7741 ccctggttcc gaatgatttc caaaggcagt tggcgaatac tttcttccaa ctgtttgata
     7801 tctgcataaa aagtggtatt actgatgcga tatgattcca tgaaatgagc aagactcaac
     7861 ggctctgtcg tcagtaacaa ctcggctaaa atcgcgtgtt gtcgtttagc agtggataag
     7921 tcattacttt tcccatctgt attttctttt aattgctcta atacttcttc tgatgcctca
     7981 atgacaaatg agcctctagc tgcttttttc aaagaagcat ttaccgattt caatgagact
     8041 tcaagattat ctaattcgcg gtaaatagtc cgtttactga ccttcaataa attcatcatt
     8101 ttttggatag atacaggtga cgggctactg agaaattctg ctaataattg tttttctctg
     8161 ggcgataaat acatcttata tttctccctt cacgagtttt tcgtattcat tcgtagctac
     8221 ccaatgttca actagaacta gccgagggat cttttcttct agctgttcag ttacagctag
     8281 ttctctttgt gcgatataaa tcgtttcttc ttggattttg attttttcta ttgattcttt
     8341 tgagataaca aactgttttt tcgctttttt tgctaattga cttaaaatcg tcatgcccat
     8401 agtttgtgag ccgcgtctgt tgtttttata caaaaataca atattagcag gcgacttttt
     8461 ttgttctgtg atatctgcta tctctttttt ttcttcccgc tttggaaatt ggatcgcttc
     8521 ttttattcgt gacagcaaga tatctgtttc caaaaagtga tccagtacat agatttgact
     8581 ttgaggtact ttttctcttg caagacccgc tagttcatgt tgcacgataa ccagctgatt
     8641 tacttggtta ttcaattcat agatagagct gtatccgaca gaaagatcga ttttttctgc
     8701 tgctaatttc tttttcagca gggcggctcc catagcactt gatcccatac ctgagtcgca
     8761 agcaaaaatg acggaatgaa tgatagatgg ttccaagatt tctccccttt ctttgaccgt
     8821 tttgttctct ttttctttat ttggtaattt caatagtatc gctgcacaaa tgaaggtcac
     8881 aaatgtactg attgctatcc ctatacttac cccagctagt tgtggataag gcgcattggc
     8941 aaaaatcacg agtagtgacc caggcgatac aggtgcagct aatcccgtat ctaaccattg
     9001 aaatatcaac gtgccgcttg ccccaccagc cattacagct aaaaatagat acggtcgcag
     9061 caagacaaat ggaaaataga tttcgtgaat cccgccaatc gcatggatca tcaatgctcc
     9121 actcgcattt actttttcct gtttttctgc aaaacaaagg atagaaagta agatccctaa
     9181 accaggtcca ggatttgcct ccatcaaaaa taaggaagag tacccgactg tttgtgagaa
     9241 ttcgattcct aaaggtgtca atacaccatg attgattgca ttattgaaaa atagtacttt
     9301 tagaggttct aagatcagac tgacaagtgg taacagattc attgtgacta gccaagacac
     9361 aacggaggcg agcaacaagt gaacactatt taaaactggt tcaataaaaa cgattccaat
     9421 tatacaaata acgctaccag tcagtcctac tagtaggttg ctgacaagca tttcatatcc
     9481 agccttgaat ttcacgtgga atctagaaac aaattgttca tatacgatgc cggcaagcgg
     9541 cccgataatc atcgctccta cgatttgtgg agcattggaa cgagcgatca accctaaaga
     9601 agcaattgca ccggcgactg cccctttttg tccccctacc gatttcccgc ctgaataact
     9661 gatcaagatg ggtaaaagca gctggatcat ccaatggcat acctcttcta atgtggtgtg
     9721 caaatcgcca gacacacctt ttgttgctaa tgacaagatt ccccaagcga taaacagact
     9781 tagatttggc atgatcattt catttaagtg atgtccagct tttctcagtt gtttcataga
     9841 cacgcgactg ctgttgtttg ctttccattt gtattccatc tttttcacca tacttctatt
     9901 tattgatgga cggattttat gtagaaaaag cagcaaacga aaaaccgtct gctgctttca
     9961 gctatctggg aactatttgc ctggttcagt cgcaatgatt gctagttcac cttggaccgc
    10021 ccactcagaa acgccatttg gaataataca gcttgttcct ttttctaatg gatatgtttt
    10081 tccgtcaacg acaaggtctc ctgctccttc gattaccgtc atcaatgtat aaggggcttg
    10141 ttttttgaaa gatgtgattc ctttaatatc ccactcatat acattgaaaa actcagtttc
    10201 taaataagtg acgatcgatg aattcccttt tcggacttct ttgatttgta attctggtgt
    10261 tttcgcaggg acagttgtta catcgatcga ttgttggata tgcagctctc ttgtttgtcc
    10321 ttgcgcatct tttctgtcgt agtcatacac gcggtaagtc gtgtcagaac tttgctgtgt
    10381 ttctaaaatc atgattcctt taccaatagc atggatcgtt ccgcttggta cataaaagaa
    10441 atcacctttt ttaactggta ctttcttcaa aaggtcatcc cagcgcccat ctttgatcat
    10501 ctcagctagt tcttctcttg tctttgcatg atgaccatag atgatttctg caccaggttc
    10561 agcatcgatg atataccagc attcagtttt tcctaattca ccttcatgtt tcatcccata
    10621 ggcatcatct ggatgtactt gtacagatag atcatcttct gcgtctaaga tcttgatcaa
    10681 taatggaaac actggttctg aaggatggcc aaacaattcc ttgtgttctg accatagttc
    10741 atctaatctt ttccctttga attcgccatt ttctactaca ctgacaccat gaggatgcgc
    10801 actgatcgcc caatcttctc cgattttatc gttagggata tcaaaaccaa agactgaacg
    10861 taaacgactt cctccccaaa ttttttcttg aaaaactggt tttaaaaaca taggttcctg
    10921 cataattcaa actcctctca tttttcatag ctattatagc attatttcgg taataccgct
    10981 ttctctttca aaaagttttc aacaatttca tctcgacata tgagatactc cgcacgatga
    11041 tcgacaggat gtacgcgatt tgacaaaaat atcaacgctt cttgtgtttt tatatcaatc
    11101 aagaaaaacg tacctgtata accagtatga aaaagaatcg catgaccgtc ttctacatcc
    11161 ttttttaagt cccatcctaa agaacgatag ccattttttt tcggtgtttg atcttgcagt
    11221 aaagaatcaa tcgtctcttc ttctaaaaaa cgaccattac ggatttttcc cttgttcaaa
    11281 tacatttgga caaaagtaat gctgtctttc aaatcaataa agagacccgc gttacctgca
    11341 tgttcagcca aaactcttgc tttcggatca tgagtttgtc cacgcaaaac ctcacccgaa
    11401 ggcagttgtt ctgtgggaac gatttttttg gaaggaaaag gaagaaataa gctgttcatc
    11461 attcctaatg gttccaatac ttgttcttga aagatttcca ctgcagtttc ttggtaaatt
    11521 tcctctagca tcaacccaag aagaatcgta ccggcatctg tatattttac ttcttttcct
    11581 agttgctcac ctggttctag cttcaaataa gcggcgatca attcttcctg agatagctta
    11641 tctctgtttg gtatccatgt tttgatatca gaagtatgtg tcagtaaatg acgcaaagtg
    11701 attgtctgat tttcaaattc aggaagatat ttatgaagcg gttgatcgag ttctatcttt
    11761 ccatcttcta atagacgtaa aactaatgaa gtggttgcaa cgactttcgt cagtgaggct
    11821 gcatcaaaca acagatcttc tgtcatcagc tgcttttcag gaacgacttg ggcaaagccg
    11881 gctgtaaatg tttctacttg attttctttt atgaaggcga ctgctacacc tggataaaat
    11941 ccttcttcca tctttttcaa aataagctgt ttcgtgtgtg gatacatctt tttctcctgc
    12001 ttttctatca gattcttaca gtccaagctt aaagcttaat ctatgaggaa tcaacctttt
    12061 ttacacaaaa aaactgcgcg aaaaaccgcg cagtttcccc cggtatacta ttttctcttt
    12121 ctatctctag aataagcgat agtatgaccg ttcataaaat gacgagacat gcgacaatcg
    12181 ttaagaaaga aaaagttaag cgtcgcatcg aatgtcagtt ttattctttt cttttggctc
    12241 actgacgaac caccattcga ttcctatagc atcatagatt gtgagtactg tgtgcttttt
    12301 gtctaagaag aaatcaactg atagttgctg caagtgagca atcagtcctt ccatgctttc
    12361 agtatcgatc atgaatttca gaaaatcaag tccaatcact tcatctgtag gcaaatcaat
    12421 tgcgcctccg ctagcttctg tcaaaccaac atggaaatct ccttcattca atacatggat
    12481 gccctcttct tcattatgta ccttcaaacc aagtacatga ttaagaaatt ggcgttcttt
    12541 tgttaagtta gtaacattta aatggatttt ttcaaaataa gaacctgttg atagtttact
    12601 gaatttttct tcagattttc ttaataatgc ttcttgatcc attgtttctg gaatttctct
    12661 ttggtcgttc ttattctctt catggtaaat ttccaattca ttctcttctg ggtctacgag
    12721 caaaatcccc atacgtccat gatcttctaa agcttctttg attggataat tcattttacg
    12781 gatacgacaa aggatatctg ccatttcttc tgtgctaggg ataatcaatg aaaaacggtt
    12841 cagctttttg atcaccccca tgtgatcatt cgctctagga ctttcttcca gcagcaacat
    12901 ttcacttcca gcttcttctg ttcctaaaat ggctaattcg ttttcttctc ttttcaaatc
    12961 aaagccaaca acgttcttgt aaaaatcaat cattttatct cggtctttca cccgaatggc
    13021 tacacttttt aaataggtgg cagcccccaa ttgaaaatct ttcatatttc ttgctcccct
    13081 ctacgtcaag ctttatttta acgatggatt cttcttcttt caagcaataa ctttgatttt
    13141 gttaaaaaaa atgtgagaaa attcgcattt tgcttgtttt ttgtaaatta atgagtaaaa
    13201 actcttataa aaagaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa
    13261 caaaaatagc tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
    13321 gaacaccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat tagagataca
    13381 aaacggaaaa atagcttctc atattttgcc gttttttgag ctaataccga aggacagctg
    13441 attgaacacc gtttattcgg aaaaagggac tgtgacacga cttttgtcac agcccctaaa
    13501 aaaaataata aatttgtact attctatgaa gcttcttctt cagaaaactg actgttataa
    13561 agatctgcat agaatccgtc tttttccatc aatgtatcgt ggtttcctgt ttctacgatc
    13621 gaaccatgat tcatcacaat aatgttgtct gcatcgcgta ttgtagacaa gcggtgagca
    13681 acaacgaaac ttgtccggtt ttccaatagg cggttcatcg ctttttgaat cagtatttct
    13741 gttcgtgtat ctacactaga tgttgcttca tcaaggatca ggacatctgg atttgctaag
    13801 aatgcccgcg caattgtgat tagctgacgc tgcccttgcg aaatattgct tgcatcctcg
    13861 tttaatacag tgttatatcc ttctggcaat ttacgaacaa aatcgtctac gtgagcagct
    13921 ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt tcccatattt gatattgtca
    13981 tagatacttc ctgtaaacag ccaagtatct tgcagtacca ttgaaaactg tgcgcgcaat
    14041 tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac gaatactacc tccagaaaca
    14101 tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc ctgcaccagt cggacctaca
    14161 atagcgacca tctcaccagg tttgacatcc aagttaaaat cagtcattag caagttatct
    14221 ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt atggtgtatc tttttcttct
    14281 ggaaggccag atttttcatt ggacatctct tcttcatcca acacttcaaa tacccgttcg
    14341 gctgaagcaa ctgttgattg gatagtgttc atcaaacttg caagttgagt gattggttga
    14401 gagaactgtg ttgtatattg caagaacgct tgaacatccc ctaatgtcat gttcccattt
    14461 gcaactttta ctccgcctag tacagcgacg aacacatagc ctagattttt aacgaaattc
    14521 atcaacggca tgatgattgc cgaaataaat tgagctttcc aaccagcggc atacagattc
    14581 tgattttctt tttcaaatac ttcctggtct ttttcagtat gattgaatgt tttaacaatg
    14641 gtatgaccac catatgtttc ttctacttgg ttattcaata aacctaaact tttttgctga
    14701 tctgcgaaaa acacttgtga tttcggcgct acgatcatca cgatgatcaa gcttaaaggg
    14761 atcatcaata acgcgactaa cgtcaattgc cagctgattg aaagcatcat cacgatgacc
    14821 ccaatcaatg tgactgcact agttacaaat tgagtcaaat tttgctgcag cgtgcttgcg
    14881 atgttatcca tatcattgat tgctcgagac atgatatccc catttgtatg tgtatcataa
    14941 taggaaatcg gtacacgatt cattttggct tccaaatcat gacgtaattc ataaactgtt
    15001 cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt tgaatacagc agaaatcaaa
    15061 tacatcacga ttactgtcgc aatgatctgc gcgattttat caaagtctat cggaaatttt
    15121 gttacttgtt ggccttgttg catctgttga gcgccagcca ttacaccttt gaatatttct
    15181 gtggttgctt tccccaagac ctttggtgtt tggatttgga agatcacggc tgctattgct
    15241 agtacaaata cagcgatgat cgcaaccata cgtttagaca tatagccaaa caatcggcgt
    15301 accgttcccc agaaattttt aggcttttca ccttttacac cgagatttct gccaggtcca
    15361 ccatgtcctg gaccgcctgc tggtccatga gagggacgtg tgtttgtttt actcatgcta
    15421 agtcctcctc tcttagttgt gaatgaacga tttcttgata agtttcgttt gttgctaata
    15481 gttcttcatg tgttcctttg ccaaccagtt ttccttcatc tagcacaagg atcatatctg
    15541 catccattac tgtacttact cgttgagcaa ctaaaacggt gatactttct ttcatattcg
    15601 ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc caacgctgag aaggaatcat
    15661 caaacacata gatatctgct gttttgacta atgcccgtgc aattgccaga cgctgtcttt
    15721 gtccaccaga gaagtttcct cctccttgtt ccacatggct atctaagcca tcttccatct
    15781 ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt ccaaatcatt tcatctgttg
    15841 catccggctt accatactgc atattgtctc ggatcgttcc agagaacaga actgcttttt
    15901 gaggaacaaa gcccattaat tctcgcaaat tatattgagt catgtcacta atgtccgttc
    15961 catttatttg gatcgaacca gattcgatgt catataatcg agggatcaaa ttgactaagg
    16021 tcgttttacc tgaaccagtc ccccctatga ttgcgacgat ctcgcccgac ttggcttgga
    16081 aatcaatatc ttccaatgca agtttttctg ctccatgata tcgataattg acatgattga
    16141 aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt aggattttca gggtcatgga
    16201 taccgatttt ttcatccaac acttcattaa tacggtcagc tgaagcttgt gcacgaggca
    16261 ccatgataaa aatagcggaa agcatcataa agctgatcaa gatctgcatc gcataagtca
    16321 taaatgcaat cagattaccg acttccaaag tcatatctgc tatgtagtgt ccaccaaacc
    16381 aagtgattgc tacgtttgta ccgctcatga tcaatgtcat cattggcatc atcaatgcga
    16441 cgatcgtatt aactttgatt gccgtatttg tataatcttt attcgctaaa tcaaaacgat
    16501 tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct gacccctgtc aatccttctc
    16561 gaaataccag gtttaaacgg tcagttttct tttgcatact tttgaataat ggaacagcaa
    16621 agtacatgat tccgccgatc aaaataatca ttactgggat gacaactaag aagatttttg
    16681 tcaactgatg atctttttga taagcaagta tgctcgcgcc aatcaacgtg attggtgcat
    16741 tgatcatcat acgtaaaaac atttgtgtaa ccatttggat ttgagtcaca tcattggtcg
    16801 ttcttgtgat caaagaggcc gttccatatt tatcaaacgc atcttttgtc agactctctg
    16861 attttttata gacatccgta cgcaattgct tacccagttt ctgtgattca cgagtagcaa
    16921 aaaatgtatt tgcgatagca gctaagatac tgatcaaaga aaagccgatc atcacaaaac
    16981 ctgtatgcca aatataatcg acatctcctt gcgctacccc tttatcaata atattggagg
    17041 taagtgtcgg caaatataaa tctgccagta cttggatcac catgaaaact gcagctgcaa
    17101 ttgctgaagt caaggagatc cgttttacga tttttagcat ctagttccct tctttctatt
    17161 tatatactcc gtttttcagc caagaaagtt tcaaattgaa ttcactgatt gcttgctcta
    17221 ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg cgcaattgtt gtaaatacag
    17281 tattcatcac taattggatc agtaattcca attcatgatc attttttact ttcagcttcg
    17341 tccgatcgat caattcaagc aactcttttg catcttgttt gtgttcttta tgtggatcgt
    17401 gaggcgtttt ttggcgactc gtatgaggag cgacttttct tgaggcacgg taatccatgt
    17461 tcataaacaa gttacgataa aaagaagcat tctcgccttc caaaatctca taaatcattt
    17521 tagaaaaata ggattcgaat cctttgaata gatcaccatc ggattctttc attagttgta
    17581 acatatcttt agagctgtca cgtctcattg tctcaaagta ataaaaataa agatcctctt
    17641 tatcttcaaa atactgatag aaactgcctc tagggatatc cgctaatttg acgatattag
    17701 caatcgaagc ttcttttaag ggtactctag aaaactcgat tctcgcagcc tcgattagtc
    17761 ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt cggcatatgg atcctccttt
    17821 ctttcaagat gacacattgt catatgacgc tgtgtcatta taacacctcc tacctgtttt
    17881 gcaagacatt tgcatagttt ttttcattat catccgatac attaaaaaag tgataagagg
    17941 aatccttcca tttgaattaa tgagagttgc ccattaaaat agccacatac taaacaaagg
    18001 atgagataaa tggcccaatc aattagtgtc caagacttca ataaattgcc tctcgatgct
    18061 gacagcatcg ttttagatac aagaaatacc ttagattaca caaaaaacca cttaagccac
    18121 tcgctttcta tccccgctgc tatcttgcca agacaattaa aaaatctaga tccaaagaaa
    18181 ctttattata ttctaagcta tacaggcagg cgatcagaag cattagccga tcaacttact
    18241 gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg aagctcttca gtcgagtgtt
    18301 gcttgaatat tgcttgttag attcaaaaga ggctgtgaca aaaaatcgtg tcacagcctc
    18361 ttttataccc tgtctttttt tagttattta ttcttcaacc ttatcaatat gacttgatag
    18421 ccttttcttt ttctcatagt tataacaatc atagcaataa aaggagaagg taagcatatg
    18481 gcatctgttt tagtagtcaa aggtcatccc ctaactgcag aagaatcgcg cacagttaag
    18541 gcattgacgt cttttttaac aagttataaa gaaaatcatc cagatgatga agtgactgtt
    18601 ttggaacttt atcgcgatga tattccggaa atcgatgaag aattattatc aggttgggaa
    18661 gcattacgtg ctggtgcaga attcacttcc ttatcagaaa gtcaacaaca aaaaattgct
    18721 cgtttcaatg aattgactga tcagttcctt gctgctgata aaatcgttat cgcaaacgcc
    18781 ttatggaatc taaatatccc aacaaaatta aaagcttggt tcgataccgt caatgtagct
    18841 ggaaaaacgt tccgctatac agaaaacggg ccagaaggat tagtgacagg taaaaaagca
    18901 ctgcacatcc aatctaatgg cggtgtatac aacggacaag actttgcctc tcaatatgta
    18961 aaaggtattt taaactttgt aggcatcgat caagtagatc agctattcat cgaaggaatc
    19021 gattatgacc ctgaccgtgc agatgagttg atgcaaaatg cattagataa agctgctgct
    19081 ttaggtaaat cattttaaaa attgagataa atgcatacaa aagagacaga ggaaaaaatc
    19141 ttcaaagcag catttgtacc ttggatattt tcccatctgt ctctttttgt tattctttct
    19201 gctttttcaa cggattttct tcataaagag aagagattgt tgtgtaccat tcaaaaacat
    19261 gagtaatcac tactttagct gctgcataaa caggaatccc caagatcaca ccgatgattc
    19321 cgaacaattt cccagaagtc agcagaacta ataaaatcgt tactggatgg attgctaact
    19381 gattccctag tactaatgga gagatcaagc gtccttctat tgtctgctct actgcaaaaa
    19441 cgatcagtac tttgatgatc atgaatggac ctgctacaat ggctaaaaag atagccggaa
    19501 tcatcgctaa aaatgatcct aagtaaggga tcaggttcaa aaaacccgca ataatcccca
    19561 acgtgacagc atactctaaa ccaatgaccg caaaaccaat cataaacatg attgccacag
    19621 caaaagccac agttagttgt cctcttatat aagaagaaac ttgactattc atttcactca
    19681 gcacattcaa cgtcggtttt tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta
    19741 attgtttacc gtctttcaat agataaaata agataaacgg catcgtcagc agagccacta
    19801 aaatagtcgc taccgcgccg acaaaacttc caagactctg aactgtactc ttagatatat
    19861 cttggatcat atcgcctgcc gaattcatga acttatcacc tgctgtttcc aactgttcgc
    19921 gaaattggtt gaacagcggg tcccgcaaaa tctcggttaa tttattatca atcgtatcta
    19981 catattgagg gaagttactg atgaagctca ctgtttgttc ctgaatctta gggatgatca
    20041 ctacgctccc ccatgcgatt agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa
    20101 gccttggtac ttttcgcttt tcaaagtaat caactaccgg attcatcaag taatacaaga
    20161 ttcctgctaa aatgataggc agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca
    20221 aatatgaaac ttttgtaaat aaaaagacga tcagcaatat taacaataca acaagtaagg
    20281 ctgtgactac ttgattattt aaaaaccagc gccaaaacca cgataccggt ctgttcttct
    20341 tattttttcc ttccataatt cctcctgtta gttataaaaa atttggcttc ccaaaccaat
    20401 agcaggcagt ctatgcggat gaagatagat tccatttgca atttgtcctt cttctggaca
    20461 agcatcaaaa atcaatgata cgtgagctac tgtctctaaa ttcccattgg ccatcggacc
    20521 gacataatcg atcgtatata cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga
    20581 aaaaggttcg tcttttgata cttcttgaat caccgaatat tttcgtaagg cagctgttgc
    20641 tgttttatcg aacaaaatca gcatgggttc ttccttgttt attgcttctg tcccaatttc
    20701 tgtaatcttt gcctgaatca ttgcactctc tcctttaatt gctttcaaaa acagtataac
    20761 acattctcat tctttagccg tgtggaataa ttatgtgata gaataaaaac gaaaactatt
    20821 tcattacaat taggaggaat cagctcatat gatcgaaaag ataattttag gaacctatac
    20881 gcgtcgcgaa agtaaaggaa tctatacgat cgaccttgat acagaaaaag aagaattagc
    20941 caacctttca ttagctgttg aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg
    21001 caatctttac acagttacta gtgtggatgg attaggtggt gccggtgcgt acgataagga
    21061 ttttcatttt ttgaatgcgg taacagagtc tggcgctcct ttgtgttatg tagcggttga
    21121 tgaagcacgt caattactat acggggcgaa ctatcataaa ggtgaagtaa atgtctacca
    21181 tatattagga gatggcgaac tgaaagcagc agattcaata ttccatcaag aagcgacagg
    21241 tcctcataaa aaccaagatc atgcgcatgt ccactatacg gatctgactc ctgaccaacg
    21301 attagtcgta tgtgatcttg gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg
    21361 caaattgaat cttgtaacta ccttcactgc agagccagga acaggccctc gccatcttgt
    21421 ctttcatcca aatggacaat tcgcttattt atttggtgaa ttagacagca cagtccgcgt
    21481 gctttcttat aataaagaaa caggtgcttt tgaagaatta cagaaaatat cgaccttgcc
    21541 agaagaattc gacggtgaaa atggaggggc agctatccgc atttcaaatg atggcaagtt
    21601 cctttatgct tctaaccgag gacataactc gattgctgta ttcgcagttt ttgaagatgg
    21661 ttcccacatt caaaacaagc aaatcatctc aacagaaggg gattttcccc gcgatttcgc
    21721 actagatcca agcaataatt ttgtcgtgtg tgccaatcag aacaccgata atctgacatt
    21781 atatcgccgt catcctgaaa caggattact ggaattactg aaaaaagatg tctatgtacc
    21841 agaatgtgtc tgtgtttatt ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca
    21901 agacgtatta gacagactac gggaagaact aaatattcct ttctttgatg ggaaaatcga
    21961 agataaagag tatagcgaag aggaatatca gaaactcaaa gccgatttac agaactactt
    22021 tgaacaatat gtacagaatg tagaaaacta atcagctttc catactatct gaaaagaaca
    22081 tcaggacaag acttttccgt ctatcctgat gttcttttct acaaataaaa aaccgccgag
    22141 atggttagac tcgacggaaa aggagtattt actcgatttt gagtaagtac aaggttgttg
    22201 ttggttatag gtatgtcatt atcataaagg attttgatca ctctatcata aaggtaatat
    22261 tacatttata ttagataact ctagttttta ttgttatcaa agatttcttt tattttttca
    22321 acaacaaatt agtcattttt tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt
    22381 ttgcttcaga tgtattgaat agatcttcat ctacccctga ttcaagatcg atactttgga
    22441 tcatttcttc acagcctgct acatatgaaa gataagcttt ttcaaaattt ttatgaatgc
    22501 ccattgcttt tgctggtgga cgcaaagtac tgactttctt aagcattgcc cgatattttt
    22561 ttgttccttc ttcaaataac aggatgatct cttcaagagt cgatttgtcc agttcacttg
    22621 tttttccttc atcaattgcg tttctgatca cttcatattt cggatgcatt ccttctccga
    22681 ttgcttctgt ttccttgacg atttgatcga tggactgcac ataatagcca atattcggta
    22741 acatgttcgt tttcctactt tctccattgt tgttattatt tattctactt gtttttatcc
    22801 tcaaatgaca agtacttgaa aaaatttaat ggtttctata cagctcgcca ttcttattcg
    22861 tgctcaaaga aaccatctgc taaactatat tgtttccctg cgacaatgtc atattggatc
    22921 aattgatatt cgtcgtaaat ctcttgttgc gagcgattga attgcgcttc tttataccac
    22981 tgtccgtttt gatagtacag ttctcgttca aacgccggaa gttcctgttc taatgataaa
    23041 agcagctgat aaaagccatt tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa
    23101 taaaacggac tggtgattgc atcgtgagtc gtttgctttt ctaattctcc tttactgtcg
    23161 aacataagaa attgtgtttc atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct
    23221 gaatagatcc ctggaagatg atccccccaa aaaacaacta aagtccttct agaaagattc
    23281 ttcaattctt ccgtgaactg cttcagtgcc gtgctgctat aggaaatatc ttgcagataa
    23341 ttctctaagt caagtgtccc acttccatct gaagtttttg caggatagtc tagattatca
    23401 tactttccat cgtagggcat atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt
    23461 ttttcttcct tgagaagtgt cattacttct ttgtaagctg actcatcaga aatatatgga
    23521 ttattctcta tcgtatccgt ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct
    23581 aatgtctgat aaacatcctc tcttttatac atcgaagtat tgtatggatg gatcgctgtc
    23641 gtctggtatg attgtgcgtt taacgctgaa acgatagaag gaagctgatc catttttggt
    23701 acaagcatcg tgtaaggggt ggtgagttgc gcattaaata acgccataga aaaaccagtc
    23761 aatgcttcaa attcgatatt tgctgttcca ccgccatagt tttgagaaag catattgcca
    23821 ctatatgttt gatcagctac ttcataataa tctgccaatg gctcaccaga tacttctacg
    23881 ccgtttaatc gactaggatc tgagaaactt tcactcatca cgaacacgat gttcggcgac
    23941 tcttcctcta cagttttatt tttttcatcg gctagcttct ggtatttttc agtgatttct
    24001 ttgatctttt ctttcgaata tccttcaggt tcatccattg gttctacctt cagattgtac
    24061 aaaaacccac cgataaaacc agtgttatag tagttcattt tctggctgta aggtatccat
    24121 agtgccgttt tattatatgc ttttcgcaac aaattatcgg gattgttgaa attactgata
    24181 taccctagca gtccaattgc cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt
    24241 ccttttttca ggcttcgaaa catgtaccag caaaacaatc ctatcacggt tcctgcggca
    24301 gctaagatca ctgtaaacag catagtgccg gtcatatctt tcagcaatcc gatttcagtg
    24361 atcatcttca aatcgtctgg ataaatcggt tcttgccgat aactcatttt catataattt
    24421 gcaaaaccta atacaccgat ggctacgaca tacgtaaggg aacctaaagg gacagatcct
    24481 gctagtgcaa tcagaaaaat aagaataatc agcaagacga gacaggctaa gaagaatttt
    24541 tccgtatgcc aagaaaaagc aaatttcaac gcaaggtcta cggatagatc attttgacac
    24601 cactgcaaaa ataaattact aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt
    24661 ccttttaaaa tctttttctg tttggtcgaa agtctgctta tatatctttt caaatgcttg
    24721 ctccttcatt tctatcaata aaagaatgtt gcttctctta ttttagttgt acgaaattcg
    24781 aaagtaaaga acggaaagaa gaattaatga aatccaaata ttctctttct ttatggcctt
    24841 tctggtttct ttaataagcg gacctttcgt actccagaaa ttttttctag ctgttgtttc
    24901 acttgattga gctgttcaaa atcagtactg tctatgtcaa tcaatatgta gacatgctca
    24961 ttcttatggt tacgcgatat atctgcgatc gctagctcgt tctcattcag cagagaaaat
    25021 atctctgcta atatattggt tttcttttga taaaatatag tgaatcgaaa cggcgcttgg
    25081 aaaaacattc gtgcggatgg ataattgacc gcttctctaa ccgtaccgaa caaaagataa
    25141 tcttttaagg cttcaactgc caaacgtccg ctgtctgcca aagcttcctg agtcgatccc
    25201 cctagatgag gcaacataag tatacggtca ttttctaaga actcttcttc tggaaaatca
    25261 gtcaagtatt tagccagatg ttgtttttcc aatgcttgca taacagcata tttatcaaca
    25321 atttcacttc ttcctacatt gataagtaca gcatcttttt tcattttttc tatattcgcc
    25381 tgatcgatca atccttttgt atcttctgtc aacggcagca tgactacgat gtaatccgag
    25441 gtagaaagaa ctgtttctaa atctgcttgc cgaacataat ccaactgggc atcgcggata
    25501 gaataaccca agacatccat ccctaagcta tagcaactta gcgctacttt tgtgcctatc
    25561 gctccaagac caagaagacc gatggttttg ccttgcaatt ctcgtcccac ataagcactt
    25621 cttttgtttt ctgcctgttc taaaatatta ggacctgtta gtgtttgtac catacgactt
    25681 gcttcgatta tcggacggga agataagagc aaacagcaca aaacaagctc ttttactgca
    25741 ttagcattta cacctggtgt attcatgacg atcgtcccgt tttcagaagc tttttcaacg
    25801 ttgatcgtgt tcaccccaac accagctctt gaaattgcta gcaaatctgg tgtaagccat
    25861 tcatctttca ctttcgtact acgaataact attccttcgg ctggcaaatc atctattatt
    25921 ttgaaatatt ccccttgtaa cttttctatc tcttgttctg gaatagcatc aatcaaatga
    25981 acgtgataca taaaaccccc tccttactaa caagtatctc gaaatttctg caattagaca
    26041 actgtttacc atttgacttc ctagaagcaa aaagagcagt tagagcagag taggctgagt
    26101 gataaaaata aggctatgac aagttactgc cataagcctt attttcgaga gcaaaatatt
    26161 gagggtgaga tatcttgata aatggtgttc cgttagctga cattgataat aagactaaat
    26221 ttcaaaaaag ttgaaaagca aatttcgtca atctcttctt atttcttaaa actaagtggc
    26281 tttttcacaa cctcttttta tctaataacc actgtttcca actcaactaa ttttgcccac
    26341 gtttcaattt gatctgttgt tagatttagc gagacaacgg tatgatgacc gccacctgcc
    26401 tgaatccatg aatggacacc ttcatggaaa ttcggtttag ttttccataa cacacgtgca
    26461 acaggtaaat taggtgcttc agtcgttggt tcaaaggctt ctacttcatt gatcagcagt
    26521 ttataatgcg taccgaaatc tgccatcgat acgactaccc cttctcctgc tttcccatca
    26581 aatacaagac gcgccggatc ttctctatct cccattgata aaggagaaac aacgattctt
    26641 ggtttcgtcg cagcaagcgt cggatctact tccatcatat gggattccaa gattgcttct
    26701 tggccactag ctagctcgta agtataatct tccatgaatc ctgtatcttt ccctcgagcc
    26761 atgattttca gcaaccgatc aatcgctgca gttttccagt cgccttcccc agcaaaaccg
    26821 tatccttctg ccatcaaacg ttgtacagct agtccaggta gttgtttcat tccatacagg
    26881 tcttcaaaat tagtcgtaaa agcgttatag cctccttctt ctaagaaacg gcgaataccg
    26941 atttcctgct gagcttgtac ttttacatgt tcctcccatg tcttttcttc ataatcacca
    27001 tagtcgaagg tatacaattc cttgtactct tcaaataatt gatcgacttc ttcgtctgat
    27061 acgcgatcga ttacttgtac aagatctccg ataccaaaat aatcaacagt ccaaccaaat
    27121 tgaatctgtg cttccacttt gtctccttct gtgacaccca cgtttctcat gttgtcacca
    27181 aaacgagcca cttttattcc aaaactttca ttataggcta ctgcaacatc catccaatca
    27241 gcaatctctt tttggatctc aggtcgtttc cagtagccaa cgacgatttt attttgttta
    27301 tttaatcgag cattgatgaa cccatattcg cggtcaccat gagcggattg atttaaattc
    27361 atgaaatcca tatcgatcgt tttccaagga atgctttcgt tatattgcgt agctaaatga
    27421 agcaatggtt tttgtagaag ctttgtcccg cggatccaca tttttgctgg tgagaaagta
    27481 tgcatccaag tgatcacacc tgctacttct tctcgataat tgacctcttt catgattttt
    27541 gtgatcgtat cagcagtaac tgctaattct tgaaggcgga ttggataagg caattggcca
    27601 ttttcattca gttcatcgac catttcttgt gcatgtttct ttacttctcg cagtgcttct
    27661 tccccatata aatgctgtga tcctacaaca aaccaaaatt ctttttctcc aatattcaac
    27721 ataattagcc actcctttta tttgttttgt ccgtaatacg cattttttcc gtgttttcgt
    27781 aaataatgtt tatctaaaat ccgttgaggc agctcttctg aaaatgaatt gagctgtctg
    27841 gtaaataaat tcatcttgca tacttcatct agtacgactg cgttcattac cgcactttgc
    27901 gcatctttcc cccaagtaaa tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag
    27961 ggatcaattt tgcgttcttt gaatgtttct atgataactt ttcctgtttc atattcgtac
    28021 ccgctgtcga tttcctgttg tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta
    28081 tctgcatgcg tcgtccccat cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc
    28141 catgtcgagt gagtatggac gattcctccg atttgaggaa atgaacgata taaaacagca
    28201 tgagtaggcg tatccgaaga gggattgagt ttaccttcaa tgatatgatt gtccaagtcc
    28261 acaacgacca tgtcgtctgc tgtcatctct tcataactga ctccgctagg tttgataacg
    28321 aaatatcgtg tttcaggatc aaaagcgctc acatttcccc atgtatattt gacgagtccg
    28381 tgtttaggca agtcaagatt tgcttggtat acttcttctt tcagttgttc cagcattttc
    28441 tatcactcct ttttcttgca attgatgaat cgctgttttt tcaatgtcga tcccctcacg
    28501 gtaacgtctc ataaatatct gatagcctgc tatttcttct gctgaaggtg tgatggtgat
    28561 tccttggtct tgtccaaaca cgtcttctgc caagaagttt tctaaagact tcccatgtcg
    28621 atccatcata taggctgcta ataacgcgat tccccacgct ccaccttctc cggctgtgtc
    28681 catcactgta actggtgcct ccatagcaga tgctagaatc cgctgtccaa cttctggtgt
    28741 tttaaaaatc cccccatgcc ccactaaacg atcaatttgg acattctcag atttcaatat
    28801 atccattccg attctcattg caccaaaagc actggctaaa tggatccgca tgaaattcgc
    28861 taaatcaaaa cggctacctg gtgtacgtac aaataatggc cttccttcat ttactcctgt
    28921 aatgttttca ccggaaaaat aaccataaga aagtaatccc ccgcaatccg tatcgccttc
    28981 tagcgctttg ttgaataata ccgtgaataa tttatctctg cttatctcca tacccaaaga
    29041 attggtaaat tcttcaaaaa tactgaccca agcattgata tccgaagagc agttattcgt
    29101 atgaaccatg cctactagtt ctcctgaagg tgtagtgacc atgtcgattt ccggatggac
    29161 attttctagt tctttttcta atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt
    29221 ccctgtccgt ttggctacac tgttcgtagc gaccataccg gttcctgcat ccccttctgg
    29281 cggacaaacc gggattcctg gtctcaaatt tcccgtaggg tcgatcagat aagcccctga
    29341 ttcgcttaac ttgccagctt cttctcctgc tactagaact ttaggcaaaa tgtgatccaa
    29401 gtcaatcgct acgtgcgctt tttcgattaa ctgaccaaaa atatttttca tttcttgatg
    29461 gtaggttttc gttgaaggat cgataggaaa catgcctgat gcatctccta cgcctagtac
    29521 tttctcacca gtaagctgcc aatggacata acctgcaagc gttgtcaaaa aattaatttc
    29581 tttcacatgt tcttctttat tcaaaataga ttgatacaaa tgagcgatac tccatctctg
    29641 cggaatattg tattgaaatt tttccgttaa tattcgttct gcttcttcag tgattgtatt
    29701 tcgccaagta cgaaatggta ctagtaactg ttccttgcta tcaaatgcta gataaccgtg
    29761 catcattgca ctgaacccaa tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa
    29821 tacttctgct gccatttttc ggtaactgac gcgcagtcct ttccaaatat cttctaatga
    29881 ataggtccag ataccatttt ctagtttgtt ttcccactca taactaccag cggcaatagg
    29941 agtaaaagat gaatcaatca atacagcttt gatcctagtt gatccaaact caatccctaa
    30001 agatgtacga ccatctatga catctaacat actttgcgca tttacttcct tcataatgat
    30061 ttaactccct ccttctcgtc aaacctgatc aaaccgtctt tttagtagcg cttacaatga
    30121 ttattgtata cttttgtacg tacatgtcaa gcggttattt acaagaatac aataaaaaac
    30181 acttgttttg tacgtacata agttataaaa tgaaattgct tacaaaatct gatcaaccgc
    30241 cttctcgaaa ttctagaagg agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc
    30301 gtttatttat ttttttggcg catttggcgg cattctattt ggatatgata ttggtgttat
    30361 gaccggcgcc ttaccttttt tacagcatga ttggggtttg gcaggtaaag cttctttgat
    30421 aggatggatc acgtcttcgg tcatgttagg tgcgatcctt ggcggctctt tatctggtct
    30481 tctttctgat aagcttggac gacgaaaaat gattcttctt tctgcactta tttttatggc
    30541 tggttctgtc ctttctgcat ctgcccctca caatggttct tattttctga ttgcagcaag
    30601 gattcttcta ggtttagctg ttggcgctgc atctgcccta gttcctgctt atatgtctga
    30661 aatggctcct gctagactaa ggggaagatt gtcagggatc aatcaagtga tgattgcttc
    30721 tggcatgctc ctttcctatg ttgcagatta tttgctaaaa gtgttgcctg aaacgatggc
    30781 ttggcgcgtg atgttagggc tcgctgctgt ccccgcgctg attttatttt tcggtgtttt
    30841 agctctacca gagtcaccgc gttttcttat gcaatctggc cgcttggaag aggcaaaaag
    30901 agtattgaac tatattcgga caccaaaaga agctgaacag gaattcgaac aaatccagct
    30961 gaatgtcaaa caagaaaaga caaccggaac ttcttggcat actttgtttt tagaaaaata
    31021 tcgttcctta gtcattgccg gtattggtgt agcagtattc cagcaatttc aaggagctaa
    31081 tgctattttt tactatattc cgctgatcgt agaaaaagcg actgggcatg cagcgagcga
    31141 tgcattgatg tggccgatca tccaaggaat cattttagtt gctggttctt tgctcttttt
    31201 ggttattgca gacaagttca atcggcgaac tttattgaaa ataggcggta gcgtgatggg
    31261 actttcattc atcctaccag ctgttttagg aactgtactg gatgctcata caaattcgtt
    31321 gttgattctc ttgtttttat gtatttatgt cgcattttat tcttgcacat gggctccact
    31381 gacatgggta atcataggcg aaatattccc actagctgtc cgcgggcgag catctggcct
    31441 agcctcttct tttaattgga ttggttcttt cctagttggc ttgctttttc ctgtgatgac
    31501 tgcttctatg tctcaagaaa tcgttttcgg catttttggg atcatctgct ttttaggggt
    31561 tttatttatt caagagatcg taccagaaac tcgtgggaaa tctttagagg aaatcgaaca
    31621 aagtgcatct aaaaaaactt atcctaaacg tatttctatc catcattctt aaagaggttg
    31681 tgacagaagt ggtcagcttc aagaaataag aaggaaccgc ttagtttcca gtcacaagga
    31741 acaatttcaa aaaacagctt ctcatgtttg atgaatattg tgacttgtga ccagttggta
    31801 ggagaacacc gtttagtgga gaggtggtga aagaaacgtt cagccttgag cgatgacaaa
    31861 agctctcaca aacattgcga cgagtaaccg taggagcaat aagaggcggt aaaaacccgt
    31921 ctcttatttt tcgttttctt tcactgtttc ccgttcaact agtacaggtt catagagaat
    31981 atttggctgt gcctgtcctt tttcaatcgt tgtgacgatc caccgtgcgg catcttgtcc
    32041 caacttttct tttggatgcg tcaatgttgt caatttttta ttccctaaat ggcttaaaga
    32101 agaatcgtct gttcctacga tcgataggtc atctggaata tgatagccat tttctgtcaa
    32161 cttatctagc attaaactag ctatttgatc gttatagcaa atgatgccat ctaaagcttg
    32221 ttcttctttc aaccgttgta ctgttttttc agcgattgtt tctcgtgttt ccgtggtgaa
    32281 agtaatgata tcttccgtca aaaaacgcac attatactgt tcacacgcgc tgataaaacc
    32341 tttcatcctg tattttcctt gaagatcgtc gatttttgtg atgaacaaca gacgctgatg
    32401 tccttttttt atcaaatatt ctgctgccat aaaaccgact ttcacatcat ctaaacaaat
    32461 ataagaggca tttaactctt cataagcagc attgatcatc accatcggaa tgccttgttc
    32521 tctcaaagca acataaagtg ctaagtttgg gttatactga ttacttttcg taggctcaac
    32581 gatcaaacca tctaccccat aagcaatcat tttcttcaaa cactctttct ccaattcgtg
    32641 atcgttattc gtactggcta aaagcaatga atacccagat tctcgtaatt cattttcgat
    32701 tcctcggata atcgacggaa aaatataatc agagagatat gtggtgatga caccgatcgt
    32761 ttttgttctg ttcaatggtg ttttttcttt cttatagctg tcatctacat atgtgcccga
    32821 ccctttttct ttacgcagat agccctcatt taccaacaca gcgattgctt gccgaatcgt
    32881 atgtctgctg acgtcatata tttcttgcag cttgaattct gagggaatgg ttgtatttac
    32941 ttcgtattcc tcacgaagga tcttttgttt tatctcttct gcaatatctt gatattttgc
    33001 catttgcctt gccccttttt atttgtacgt acatatatca taacgagtct gcaaggttta
    33061 ttcaagcaaa aatcaaggtt tcttgtgctc ataaggcata cggaacaggt tcaccaaatt
    33121 ctggaaatcc ttgctcattc cacgtaaagc attgcgcgtt cgcgtgccga ttaggattgt
    33181 ctaacggatc ccctttttgg ttcttctctg gtcgagcatg atagatcaaa atatcaaagc
    33241 acccgtcttc tgaaaccgta aacgagttgt gaccaggacc aaattgctta tttttttcag
    33301 aagaaacgaa tactggctcc gataatttat gccatgattc ttttaataaa agattcttgt
    33361 gctcatcagc ccacagcagg cccatggcat agttttcatc tgttgcacta ccagaatacg
    33421 taatgaatat tttcccattg cgagtaagga ctgctggacc ttcatttact gaaaaacccc
    33481 gtttttccca ttcatactca ggtatagaaa ggaggatctg cttccccttt aacgtccaag
    33541 gattatccat ttcggataga taaagattag aattcccagt aatttcagga tcttgttggg
    33601 cccacacata atatagcttt ccttgagaag agaaagttgt tccgtctaaa ctgaaagttt
    33661 cccttttcgt ttttatttgt cccttttcta tccaatctgt attcattgga tctatatgct
    33721 gattttcaag tacgaacatg cggtgatgat gagattcatc acgtatagtt ttatgattgc
    33781 ttgcagcaaa gtaaatatac cattttccat tgatataatg gacttcaggt gcccagatca
    33841 attcactcat cggtccttct tcatgagcct gccatacaat caggcttgca gcatgttcta
    33901 attcattcag tgactttgcc cttcttaatt caatcacttg gtagcctgga accgatcctg
    33961 taaaataata ataaccatct gtatgtttat atacccaagg atctgcccgt tgccgaacga
    34021 taggattatt ataaagtgtc ttcatcataa caaatttcct cacattttct ttattctaat
    34081 tacattccaa gagtattttt ctatccctac atttaattcg tcatgttttt ctattgaaaa
    34141 atagtttatt tcaggtttta ttttttctgg ttcttcacgt gtatttttgt ccaacagatc
    34201 gctactggtt aatgtgtagt gctctccttg atctagcaaa ttgaaatcat gttcaatcgt
    34261 tatcttttgt tgttcatttg tgcgattgat caagaaaata gtcagctgat tgccgttatc
    34321 tacgatcaca ctgtccacat aaggaacatt ttttatcttt tcacaatcat aaacagggca
    34381 aatgatttgt tgtttcaata cttgaccttt agcatatttc gataagtgtt gcatcacata
    34441 ataagttgga ttcctccatg cttgtccttt agcatctgtc agtattaaag gtattgtgtt
    34501 tactaacaat gattgacaac taatctttac gcgatctgcg tgtcgtaaaa tagccagccc
    34561 taatgaacca aataataatg tatcttccat agtaaagtaa caccaatcga tgggactacc
    34621 gacttcaaaa tccctatgtc ttttttccgg ttgggcatgt acattccatt catcaaacgc
    34681 tagatacatt gttttctcac ttctcaaata tccttttaca taatcacaag tagcaacgat
    34741 ttcttcaatt tgacgatcga atcgataagc ttttgcaagg tatgtcgctg tatcatcacg
    34801 ctcattttta ggaggtttag tcaagtcttc ttcatcatac ttatcaatat agttatgcaa
    34861 agctaaataa tctacattct cataagcttc ttctaatgcg atacgatccc attcagggta
    34921 cgatggtaaa cgtggcgtag agcttccaac taagattgtt tcaattgaat catccactaa
    34981 cttcatgact tttgaggctt catttgctaa cctaccatat tcataagcac tcttagcccc
    35041 cacttgccat ggaccgtcta gctcattccc taaacaccat aatttaacgc caaaaggctt
    35101 tttttgccca ttttttattc ttaaatcact ccagaaggtt ccttttggaa aattacaata
    35161 ttctactaaa ttttttgctt catcaattcc ttttgttcct aaattcaccg tgaatatcgg
    35221 tttcgcttgt acatgactga tccatttaaa aaattcgtgg agtccaaaag aatttggttc
    35281 tatacctcgc catgctaaat caattttttt tggtcgttgt tctttcctac ctacagtatc
    35341 cacccaatca taaccagatg taaagtttcc tccaggatag cgaatgacac ctaattctaa
    35401 ttcctttaca taagagagga catcttgtct aaacccgtca ccatcagctg tttcatggcc
    35461 cggttcataa atccctgtat agacgacact tcccatatgc tcaacaaatg agccaaacag
    35521 ccgagaactg atctctccta ccgtaaaatc ctctctgatt tttactttca cttcttcgtc
    35581 caacagcttt ccctaccttt catttcctat agtcaagata atagatacaa gtttccatac
    35641 gccaccagca aagataaaaa taaaaggagg gaaaagaaat atgagcagta caaatcctaa
    35701 taacaatacg caacaaagaa tatttctcaa aaaatcaaaa aatactttcc atgcactttg
    35761 ttcaaaaatt tgtaaaaatg tgatatcttt gttttcagaa atcacatata tccaggataa
    35821 aagaaacatg gaataaataa gtaaaactac atatccacta attgaaagta cgattgtctg
    35881 ttcttctaat aattgattaa caagaaaata aataatgatc gttaacagac taaggaacaa
    35941 gttcttacca aaatgttgct ttattccttg aaagaaaaaa taaatgatat ttcctgatcc
    36001 ctcacctttc cattgcgcca ttacataaaa aacagcacat gtagcaggca cgatagtaat
    36061 aacaggcaaa cacacgatta cccagagaat acctaataag atgtaatcag aaaccacaat
    36121 caactgtcca taaaatttag tctcaactat tttcatcttc ctcactcttt cattccagac
    36181 atagctgttc cttgaacaaa atatttctgt gcaaaaatat aaaccactag taatggcagc
    36241 attgaaataa ccgttccagc cattacaggt ccataaaaag aaatgttctg ccctgtaaat
    36301 aaagctaacc ctgctggtaa agttctcatt tttgaactac ttgtcaatat caatggataa
    36361 agcaagtcat tccaactatt catcagcgtg aaaatcccta atgataaaag agtaggtttg
    36421 ctcaatggca acatgatttt caaaaaaata ttaaactcac ttaatccatc aatgcgtgct
    36481 gcctcttcca aagaatctgg taaagtaata aaaaaggaac gcatcatgaa gataccaaat
    36541 gcagttgtca tacgaggaat gatcaaacct gcataagtat caagcatgcc aagcaaattg
    36601 acttcaataa atagaggaat catgaagact tggaatggga tcatcattgt cagcaaaacg
    36661 aaatagaaca aaactgattt tcctttgaaa cgcatacgag caaatgcata ccccgcaaga
    36721 gaatcaaaga atacagaagt gatcactgtc cctccagcaa aaatcactgt atttttgatg
    36781 taatctaata aagggatcgt tgtccaaacc ttaatatagt tatctagggt atagctatct
    36841 gcaaatattc taggcggaaa tgagataatg tctttttcgt gtttaaaaga agaaataatc
    36901 agccacaaaa aaggaaacgc aataaccaac aatataacta gcatactaat cgttcgaatg
    36961 atttttttac cactaactgc ttttcgacta ttcacagtaa ctgtaatcga gctttctggt
    37021 gccatacagg tttccctcct atttcatttg tttttctttc cgtgacatgt acacattagt
    37081 ggagatagct atagttgcaa caatgataaa caaaaccgaa gacagtgctg atgcatagcc
    37141 caattcataa ggtgctgtaa atcctcgact gtaaatatat tgaacagctg tttctgtctt
    37201 aaattgtggg ccaccagcgg ttgcaacata tacttggtca aacacttgca tcgaaccaat
    37261 cagattcgtt aaaatacaaa aacctaagga gggaacgatc tgaggtagcg taatgttaaa
    37321 aaactgctgt cgatttgttg caccatccat ttgagcagat tcatacaaag agggagagat
    37381 tccctgaata gcagtcaaga gaatcgtcat agtaacacca aaattcttcc aaacagtcat
    37441 gatggctacc gtcggcatag caagggttgg atcgcgaaaa aattgaggga tcggcaagcc
    37501 gacttgtgaa aacaagtaag gaatcgcccc tacattcgga tcaagcaaca tagaaaaaat
    37561 aatcgcaatt gacgtcaatg aacaaataac tggaatgtaa aatgtcgaac gacaaaaacg
    37621 attgaatctg gtatttttag ataatgctac tgctgctaat aaaccaacga taatttgtgt
    37681 tggcgtttcg agtacggtga aatataaaga atgaatcaac gaattaactg ctcgttcatc
    37741 actaaatata cgtgtgaaat tttcaaaact tacaaaattt cttgatgtaa agaaaatatt
    37801 caaatctgta aaactaatag caaatgttcc taaaagcggt atgacagtaa ataataataa
    37861 aactaaaaaa gatggagtta aaaacaaata cgcagcacga tcaggacgat tccaatattt
    37921 ggtgaatccg ttcatatagt ccacctcatc tttcattttg ataaaatata caatcttggt
    37981 ttaaacgaat ctgaataaga tatccgaaca atcgctgtga tttttgcaat aacacctatc
    38041 tgcaaatact aggtcgatca ttcagatatc tgtatcttgc caacagtcta tcctgtataa
    38101 agttctgtac tatttttgca gtaattgatt gatggcatca tctgcatcgt tcatgagtgt
    38161 ctcaggatct tctcctgcta ataatttttc aatcagtgga ttgatgatat cgttattgat
    38221 cgcagagatt tgggtaaaac ctggtaaata tggctctgca tagtccattt gtttcgataa
    38281 ttcagataca ataggatcat tttttacttc ttcatctttt gctactgaat ccaagtaggc
    38341 aggaaaacca ttttcgatac tccatttttt accaatttca gttgtattcc aatatttcaa
    38401 aaactcatag acagcttctt ttttattatt atctgtactt gccggaatgg catatcctac
    38461 gccatctaaa atagctgttt ttttgtctga atcactccct tgtggtaagg tagtcacatt
    38521 ataatcaatc tcgtttgctt tcaatccgtt atttaaccat ggcccattga tttcaatggc
    38581 tagttgacca gcattcatta aattgtccat ttcggcgcca gaaatcgatt ctggaccagt
    38641 cttgtctaca tggatcattt tttggatcgc ttttaacgtt ttaaggttct caggagacgc
    38701 aaattcagac ttagtataat catcgttcac taatttaccg ccatttgcta acatccagtt
    38761 atataacggt gcagttccat ccttattgaa gacaaaacct gatacatttt tactactatc
    38821 agttaatttt ggtgccattt ctgtcaattc ttcccaagta gtaggtggtt gttcagtatc
    38881 taatccagct tgtttaaaca aatcattatt ccaatacata tacatgcctt gtacctgcat
    38941 ggggacaaaa tactgtttac cttctacttc acctaattca atagccgttt ctttaaaatc
    39001 agatttatct actccatcat attcccagaa atcgtctaat ggttgtaccg ctccgttttc
    39061 tacatactgt gcaaaatctg gataatttaa tgcaataaag tcaggtgctt ttttagaaga
    39121 aatcgctgta ggaagttttt cattcaaatt ggcccaagtc atgatatcca tttcaatttt
    39181 gatgttttta tcatttgttt tattaaattc attaacgatt tcttgcaata tttcaccatc
    39241 agatgcagta aacccattcc aaaaagtcaa agtcacttct ttatcagaat cagattctgc
    39301 tgaacttccg ccaccgcaag ctcctaaaat acctgttgca gctaccaaca aagagccaat
    39361 taaccatttt ttacctttca ttttttgttc ctccttcatc tgataaacga catctgacca
    39421 cattccaaga tagcggtttc aataaacaat tgattttccc atcttcagct tttacatttg
    39481 ttgaatgact tggttttact tgttcacttt taggaagatt agttgctttt gtatcaaatc
    39541 cactcatttc agaaaaatca atcactccct ctaatgataa ttcagacaag tgaacagtta
    39601 gattcatttc ttcttctcca cggttaactg caaatatgac aagttctttg tcatttatta
    39661 cggctatact ttctaaatac ggcacttctt caaaatcttc cgtcgcatag tttgctgaac
    39721 gaatttgtgg gcttaacacc tctccttgtc catacacagc agcttgcata aatggataat
    39781 agatcgtctg tctccaagct tctccgtttg tttctgtcct gattggtgca ataacgttta
    39841 ccaattgagc aagacaagca atctttactc tatctgcatg tttcaataaa gtgattaata
    39901 gacagcctaa tagtaatgca tcttcaaaat tatagttatc ttctagtaaa ggaggagcaa
    39961 tctgccaagg cgctgcttgc tcatcttgtt ccgctgaatg ataccaaata ttccattcat
    40021 caaaggacag gttgatctgc ttcttactat gtttcttcgc cttgactgta tcgcacatcg
    40081 ctgtcacttc agaaatgaac cgatccatgt ctaaggaacg tgcaagataa tgagaaagat
    40141 catttttttg gttgccataa tattgatgca gagacaaata atctatttga tcgtacgctt
    40201 gatcaagaac tgttaactcc caatcaccaa atgtaggcat ttgccgattc gaacttccgc
    40261 ataaaaccac ctcaattgaa tcatctacga gtttcatcgc cttagccgtt tcctctgcta
    40321 accgtccata ttcttccgct gttttatgtc caatctgcca aggaccgtcc atttcattcc
    40381 ctaaacacca tgtcttaatt ccaaaagggc tctcttgacc attcgaacgc cttaaatcac
    40441 tccaataagt tcctttagga aaattacaat attcgactaa attacgagct gcatctactc
    40501 cacgagtacc cagattgaca gccatattga tttcggcccc cacatgctga caccagttat
    40561 aaaattcatg aatcccaact tcattcgttt caattgtccg ccaagctaaa tccaaccttc
    40621 taggtctatt tgctatgggc ccaatactgt cttcccaacg aaatcctgac acaaaatttc
    40681 caccaggata tctaatcatg ggaatgttca attcttttac caagtgaatg acgtcttggc
    40741 gaaatccact tttattagct gatggatgat ctggctgata aatcccttca tatactgctc
    40801 gtcccatatg ttcaataaat gaactgtaca accgcggatc aactttgcct acacgcttga
    40861 cactactaat caacattgtg gcttccataa ctgacctcct ttaatttata catgaataat
    40921 acacctcgtt gaa
//
LOCUS       DARDHA010000011        37213 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000011 DARDHA010000000
VERSION     DARDHA010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.011"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            208..678
                     /locus_tag="SDA10_000471"
     CDS             208..678
                     /locus_tag="SDA10_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HEG4239402.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            1307..2410
                     /locus_tag="SDA10_000472"
     CDS             1307..2410
                     /locus_tag="SDA10_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEG4239403.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            2694..3374
                     /locus_tag="SDA10_000473"
     CDS             2694..3374
                     /locus_tag="SDA10_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG4239404.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            3464..4498
                     /locus_tag="SDA10_000474"
     CDS             3464..4498
                     /locus_tag="SDA10_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEG4239405.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            4512..4847
                     /locus_tag="SDA10_000475"
     CDS             4512..4847
                     /locus_tag="SDA10_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239406.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            4831..7311
                     /locus_tag="SDA10_000476"
     CDS             4831..7311
                     /locus_tag="SDA10_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239407.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            7367..8926
                     /locus_tag="SDA10_000477"
     CDS             7367..8926
                     /locus_tag="SDA10_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4239408.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            8923..10287
                     /locus_tag="SDA10_000478"
     CDS             8923..10287
                     /locus_tag="SDA10_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG4239409.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            10458..11528
                     /locus_tag="SDA10_000479"
     CDS             10458..11528
                     /locus_tag="SDA10_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4239410.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            11543..12211
                     /locus_tag="SDA10_000480"
     CDS             11543..12211
                     /locus_tag="SDA10_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4239411.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            12231..12809
                     /locus_tag="SDA10_000481"
     CDS             12231..12809
                     /locus_tag="SDA10_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4239412.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            13212..14123
                     /locus_tag="SDA10_000482"
     CDS             13212..14123
                     /locus_tag="SDA10_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG4239413.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(14379..15296)
                     /locus_tag="SDA10_000483"
     CDS             complement(14379..15296)
                     /locus_tag="SDA10_000483"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEG4239414.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            15498..15935
                     /gene="zapA"
                     /locus_tag="SDA10_000484"
     CDS             15498..15935
                     /gene="zapA"
                     /locus_tag="SDA10_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEG4239415.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            15993..16540
                     /locus_tag="SDA10_000485"
                     /pseudo
     CDS             15993..16540
                     /locus_tag="SDA10_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            16655..19015
                     /locus_tag="SDA10_000486"
     CDS             16655..19015
                     /locus_tag="SDA10_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEG4239416.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            19102..19416
                     /gene="trxA"
                     /locus_tag="SDA10_000487"
     CDS             19102..19416
                     /gene="trxA"
                     /locus_tag="SDA10_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEG4239417.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            19598..21376
                     /gene="uvrC"
                     /locus_tag="SDA10_000488"
     CDS             19598..21376
                     /gene="uvrC"
                     /locus_tag="SDA10_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEG4239418.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            21521..22420
                     /locus_tag="SDA10_000489"
     CDS             21521..22420
                     /locus_tag="SDA10_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4239419.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            22413..23675
                     /locus_tag="SDA10_000490"
     CDS             22413..23675
                     /locus_tag="SDA10_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4239420.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            23838..25736
                     /locus_tag="SDA10_000491"
     CDS             23838..25736
                     /locus_tag="SDA10_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEG4239421.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(26035..26754)
                     /locus_tag="SDA10_000492"
     CDS             complement(26035..26754)
                     /locus_tag="SDA10_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEG4239422.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            27091..27423
                     /locus_tag="SDA10_000493"
     CDS             27091..27423
                     /locus_tag="SDA10_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239423.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            27410..29413
                     /locus_tag="SDA10_000494"
     CDS             27410..29413
                     /locus_tag="SDA10_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEG4239424.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            29432..29902
                     /locus_tag="SDA10_000495"
     CDS             29432..29902
                     /locus_tag="SDA10_000495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEG4239425.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            29923..30507
                     /locus_tag="SDA10_000496"
     CDS             29923..30507
                     /locus_tag="SDA10_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEG4239426.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            30522..31508
                     /locus_tag="SDA10_000497"
     CDS             30522..31508
                     /locus_tag="SDA10_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEG4239427.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            31498..31809
                     /locus_tag="SDA10_000498"
     CDS             31498..31809
                     /locus_tag="SDA10_000498"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEG4239428.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            31831..33612
                     /locus_tag="SDA10_000499"
     CDS             31831..33612
                     /locus_tag="SDA10_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEG4239429.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            33605..34981
                     /locus_tag="SDA10_000500"
     CDS             33605..34981
                     /locus_tag="SDA10_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEG4239430.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            34988..35623
                     /locus_tag="SDA10_000501"
     CDS             34988..35623
                     /locus_tag="SDA10_000501"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEG4239431.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            35653..37008
                     /locus_tag="SDA10_000502"
     CDS             35653..37008
                     /locus_tag="SDA10_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HEG4239432.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
BASE COUNT        12331 a         6371 c         7992 g        10519 t
ORIGIN      
        1 agtatttgtt ctttttatat cgacaatcct tgcttgaata ctatgcgtat ctcttatatg
       61 ctagagaaag aaaagtagaa gaataaggga tagatggaat ggataaatag gatttctgag
      121 gaaaaaagca catgatagcg ttgataggag tcacctatgt caggtgataa aaatacataa
      181 gtagaaaatt aagaggagga agagaaaatg aaagaagttc aattgattag aaaaagcgaa
      241 ctatcagaag gcggttgcaa tgcatgtggc gtagtagaag cgacaagcta tacattaaaa
      301 ttagattcca atcaagcaat aatctctgaa ctgacagtag gcggtcttgt ggacagtctg
      361 gcgcttgctg aaggttttac tggagaagat atttatgaga tgttttcaga agtccgtcag
      421 ttaaaaaaag gagaaaaatg catagaggtt catcatgaga gcccgaatgt gaggttcaaa
      481 cgtggtgata atgagatgat cttcaagaat catgtgtctg atcacactga actatatgaa
      541 atcgtgaatc aaatactgtc tgggattttt gaattaggac cttatgagtt caaggaagaa
      601 aacggtaatc caaaattaaa tgaagaatgg caagaaacga ttgaaacaca aagaaacaat
      661 ccacacttat ttcaatagtc ttccacagta tttcgtaaaa atgttgatga aaaagaaaca
      721 ccgtgaatca gaggtcgtga aaaacctgcc ctgcatcaag taataagaac agccaaacaa
      781 aaatagctcc tcctattttt cgtttggttg ggcttattac cgcagatgca agtttttgaa
      841 caccgtttaa ggaagagact gtgaagtcaa cgttctgacc tgagtattag agagacaaat
      901 ggcaaaatag ttcctcatat tttgccattt tttgagctaa taccgagggt cagctgattg
      961 aacaccgttt tatcgagagg ttgtgaaaaa gtcgcttagt ttcgagtagt aagaaaaatt
     1021 ttgtgaaaat ggctttccac atttttgcat aaatttgact taataccgag aaaatggctt
     1081 ttgaacaccg tttattcaaa aaaagggact ttgacactac ttttgtcaaa gtcccatcgg
     1141 ttttttatct atttttatta caatcatttc ttccagagta gtgacatctg gaatggctga
     1201 aatgtaaaca gattatttgc ctgtatcaat gtcgaagagt aatataaaaa gtgtaaacgc
     1261 tatcatattg ttcatcattc aaattttata gagagaaggc attgagatgg aaaaaacata
     1321 tcaagcaggt acaaatgaag gaatagtaga ttttatcaat atggaagatt tagaaatcgc
     1381 ggcttcacaa gtgattccag ctggtggtta tggttacatc agcagtggtg ctggcgactt
     1441 atttacttat caagaaaatg aacgggcatt caatcatcgt ttgattattc ctcatgtatt
     1501 aagagatgta gaattacccg atacaactac acattttgac gaagaaatgc tgactgcacc
     1561 aatcatcatg gcaccagttg ctgctcatgg attagctcat gtgaaggcgg agaaagcttc
     1621 tgcaaaaggt gtagcagatt ttgggacgat ctatacagca agttcttacg cttcttgtac
     1681 tttagaagag ataagagaag caggcgggga gaaagcaccg caatggtttc aattttatat
     1741 gagcaaagat aatggaatca atttggatat tcttgaagtg gccaagcgta atggagcaaa
     1801 agcaatcgta ttgacagcgg atgcaacagt tggcggaaac cgtgaaacag accgcagaaa
     1861 cgggtttacc ttcccgctgc caatgccgat cgttcaggct tatcaatcag gagtaggcca
     1921 aacgatggat gctgtatata aatcttccaa acaaaaactt agtccaaaag atgtagaatt
     1981 tatcgctgct cattctgatt tgccagtcta cgtgaaaggt gttcaatcag aagaagatgt
     2041 ctatcgatca ttagaatctg gtgctggtgg aatttgggta tctaaccatg gtggccgtca
     2101 attagatggg gggcctgctg cgtttgattc gttgcaatat gtggcagaag cagtagataa
     2161 acgtgtacca atcgtgtttg acagcggtgt acgacgtgga caacatgttt tcaaagcaat
     2221 cgcatcaggt gccgatcttg ttgctattgg cagacccgtt atttatggat tgtctttagg
     2281 aggaagtaca ggagtccatc aagtgtttga tttctttaaa acagaattgg aaatggtcat
     2341 gcaattagct ggaacacaaa cagtagaaga tatcaaaaaa ataaaattgc gtgagaatcg
     2401 ttttatttaa gaaataaatg acgaaaaaaa taggagtgcg gcagatgatg ccgtactcct
     2461 atttttatag ttcaaggatg tcactaatag aataatttga aacgaaaaaa taaaattgaa
     2521 atattttttc atacttaata aaaaaggaag aatattcatg taaacgattt tattgttttt
     2581 ttctctttta tacaaaaagg gtcataattt caagtaagcg gatgaattca tgaggatttt
     2641 ttgcatactg tctctactat acttaggtca ctaagaaaaa ggagtgattt attatgaaaa
     2701 agaacatgat tgggacatta ttactttcaa cacttcttct aggcggcttg gcaacaccag
     2761 cttttgctga gggtcaagcg acttcaaaag gagatattac ctttactgaa ccaacaaata
     2821 ccgttgagcc tcttaaccct actgatcctt caaaaccagt agagccagca gatccggaaa
     2881 atccagcaac tggccagaca ggttctttaa cattggatgt agttcctgaa ttgccatttg
     2941 gtacacacga gattgaaagc ggaacaaaaa cgtatcaagt agacgcttca aaaaacgata
     3001 caccttatct ccaagtatca gatcgtcgag gtgtaggtgc agatggccaa gcacaagggt
     3061 ggaacgtgac agtttcggtt tcagattttg taaacggttc acaagttttg caaggcgctg
     3121 aattagattt tggaacatcc actgttaaaa gtacgtcaga taacgaatcc actggaccaa
     3181 ctagtcaaac tgtaactggg ttgagtaaag caagtgcagc gacaccaata tttacagcag
     3241 caaaagatca aggtctggga acatggcttt ctgtttatga tccagcaaat atcacactga
     3301 aagtgccgaa agcagcagct ggaacattca cggctgattt gacttggaac ctcgtggctg
     3361 gcccagtagc atagagtatg aaataaattc aacaaactgc ggtgaaaata ctataggtga
     3421 aggatttttt accgcagttg ttgttgaaag gatgtggtgg acaatgaatc aaagtaaacg
     3481 actgtttgtt tcgttttttt ctattttaag tctatttttc atttttccgt caatcagcca
     3541 agcaaaggat tcagcaggag actttggaat caaaccggta tttccagaga accaaataga
     3601 taaagcaatc ggttattttg atttattagt ggcaccggaa caaaatcaga cactagaagt
     3661 aattatcagc aattcttcag atgaagaacg aacatttgag gtctctgtca atccggcggt
     3721 aacgagtgat ggtggaacga tcgattatag tcaaaaaaac ccaacgttag atgagacatt
     3781 gccgtttgat gtccgtgacg ttttgctcat tgcgaaaaag gaaatcaatg tatcagcaca
     3841 tgcagaaaca actataccta tagaagtgaa aatcccagca aaaagcttta agggacgtgt
     3901 attagcgggt attcatgttt cacctaaaga agaagctgaa acagaaaatg ctacagaagg
     3961 agcacaaatc aaaaatcgta ttgcttataa tctcgcagtc gttttacaag aaagtcaaga
     4021 gacgatagaa ccggatctga aacttttatc aggagatttg gacgaggtca acgcaaagcc
     4081 aactgttcag cttcgtttcc aaaatcctca gcctaggatt atcagcaatt tgatatttac
     4141 gagcaagatc ttttatgaaa atcagcttta tattgaaaat acgtctaatg aatttttagt
     4201 tgcaccaaat tcgaattttc acttgaatct tgatttagct ggtgataaag caaaagccgg
     4261 cgattaccgc gcagaaatca tagcaaaatc aggcgacagt aatgaatggc gatttacaca
     4321 aaactttacg atcaaaaaag aaaaagcaca aaaagtaaat gaaaattccg tttttgcagt
     4381 acaagaacaa tctttcccat ggctttatgc aggattggtt gctgcagttc tagtgttatg
     4441 gttgattttg ctgtttttgt gggtccacaa gaaaaagaaa aaaggagaaa caacatgatg
     4501 aggtgatttc gttgaaaaca tcgatcttat tgggagggat tattgcaagt ttgctgttct
     4561 tcagctggca agagccagtc caagcagata ccaaaagttc aggtacgatt acattttatg
     4621 atctaagtcc agttccgcat ccaagtggtt cagtcaatgg cgcatcaaaa cagcagacac
     4681 agctaccgat ttataaagaa tctaatcaac atggcgtatt cccggagacg aatgaagaaa
     4741 agatccgttt tctttatttg atcattggta tagaattatg gcttcttgcg tggttgttgt
     4801 tatggagaag aaaggaaaag gaggatgaac atgttgctac aaaataaatt ttcatttttt
     4861 cgctccttct tttttacttc gctttttgtt ttaagtatat ttatcttttc accttcggca
     4921 gaagctgttg gagcaacgcc tcctgccatt tcttatgatc catacacgta tgaaaatatc
     4981 gtacctccaa atggtggacg tagtgcagtc aatggcggga agcttgtcca actaactggg
     5041 gctaaccaat tgcctaaatt gactgctact tatttgaacg atggttcaaa tgatccttgg
     5101 aaaggtggca cggtccgttt tcattgggaa agccatgtga aactaacaaa tgtacttgat
     5161 ttggtgaata tcactgcaca aggatttgca tttgtggcga tcatcaattt gccggaacat
     5221 gtatcaagca gtgatgtatt aaaagcaatt gactggaaca acgcctactt gtatattgcc
     5281 ggtgccccca tcaaggttac tagcggtagt ttattatcag caggaagcca taccttgcgt
     5341 tttgggatgg ggtcgtggga tacaaatact ctattggcag atattattgc tattattacg
     5401 cgaggcgaat tcaacgtgga cgatatcccg cttcaatttg atgttacagc taatgtcgcg
     5461 aagatgacag aaaacggaga taaatatgat acgagtccaa acaaaatttt gaccaagggt
     5521 aaattacaac cttctatgtc taaaaaggcc gttttttctg ttgattttta tgattcgaat
     5581 aatattatcc aagatcgtat gttcaattcg attttagctc cagctgcagg tagattttat
     5641 cctaaattaa ctggcggaaa cttgaatcca ggagcatatg cattttctgc aagtgcagag
     5701 ataaatacgt ggaatagtta catctcaaca agtgattcaa caggaaaata taatacattt
     5761 gctgatacca cagaatacgt ggatggaaat aaccagaacc ttccaggctc taccaatagt
     5821 cggacattga cgatggggct gactgaagga cgttttgatc agttgccatt tagtcgcttt
     5881 gatcgcgtag tcaactattt tacaggaaaa aacgttacac aaggagcaat actgactcat
     5941 actccagcaa gcacaagtgg attgaatcag acagccaata tcgtgtacag tgggaaagat
     6001 gcgcaaggcg gattgctttc tcctgttgct ttaaaggtat tggagcagta caagctagac
     6061 ggaacggttt taccaacaaa cttaacagcc gatagtcagt ggggaaagct cctttcttct
     6121 gcatcgtacc aagtcacggc caattggtca gcaactgaac taaaaaaggg ttcgatccat
     6181 tacaggcttt attcgaaagg aacccaaaaa ttagcgacag gatggacaga tcaacttttc
     6241 caaacgatcc aatcaaataa cgggggtgta aatacagcta cttctacact acctgcattg
     6301 gcttcagggc aatattattt tgattatcgc ttggtggacg atgtacttac taacatgtat
     6361 ccgtcttttg cgtacaaatg gcagtctgaa caagctggaa tcaccactct tcctcaaata
     6421 acagtggcta attttccatc tgtgagtgca gatagtcagc taaaaaacct tagcagacaa
     6481 cctgagacag gagatcccct gtctgctcta tcaggagata cgattcaaga aaatgtgaca
     6541 ttcacgttaa caaaatctgg ggatgcgata accgacaaaa aaatcacgat ctcacttcct
     6601 gaaaatacta cttatatcaa tggttcctta aaattaaatg gtacaactat cgcagatacc
     6661 ggcattcaac aaggagtttc tgtcccagca gatctattga gcaaaatagg agataccatt
     6721 catcttactt acaactatca attaaatact gtagatactt ctgtacaatc cgtcagcata
     6781 ctgacaaagg cagctgtact ttccagcaat attacattag cagatggtgc taagcttcct
     6841 aacccagttg tccaaacatc tgctaaaaca attcttgtcc ccaaacaaga attaacatta
     6901 gttaatgtac cagatgattt tacatttggt aacgatctcc ccaaaccgtt gaagacaagt
     6961 tattatgaag caaaaggcga tttttctttt gatgtgcggg atactcggct tccaagcacg
     7021 tctccttggc aactgacagg gacattgact tctttattta agaatgatca aggtcaagaa
     7081 ttaagcggaa caaaattata cttcaatcat tctggatcaa aacaattgat tcagcaaggt
     7141 caaaacacgt tgatttatga gagtgatgga acagctaaag gagaggttct cgttgacttt
     7201 cccgataccg atggtttact cttagaggta aatagcagta cgaatgctca acctggcgca
     7261 acgtatcaag gcatggtcac atgggaactg acagctgggc caaccagcta agtgattaga
     7321 atttgtatgg atctgtgata actgtcacag atcctctttt tcaaggatga gaaggaaact
     7381 tctgctgtta aaatcagaaa tgcttggagg agtggacttg gaacttttca gtttgcttga
     7441 aaaaaatgaa caacggcaaa tcaaactttt ccaactttta ttagaggaaa aaggagagat
     7501 ttccgttaat aacgtactta cccgcttatc agttgatcgg gctaccttga aagaagatct
     7561 gttttattta caagctaact tggaaaatat caaacaattg gaaattgtta tccaagaagg
     7621 gaatatttta ttaatcagac atggaaatat agcagcagtc gatgtttatt atcattgcta
     7681 cttgcgctat tctacaaaat atcagatact tctttatctg ttggaaaatg gctcatttga
     7741 tcggcagaaa cttgtcatgg aacttaatat cagttcggct actttgtctc gcagagtgaa
     7801 ggaattaaat gattctttaa atgagtttca tctgaaaata aaaagcgggc ggctgaccgg
     7861 tccagaaagt caaatttgtt atttttattt ccaattattt tggtttggac gtccttatct
     7921 tgaaaacaaa aaagaataca gtgatttatc aggaaacact tttttagatc tgctgcaaaa
     7981 agagtttagt tttactatta cagaagaagg acgattgaaa tttgaaatat ggctttccat
     8041 tatgaagaag cgaatgcgct gtacgaaaaa atccaaacca gattatagca atcttcctgt
     8101 tggattgttt caaacgacac aatttttatt ggacctccat acatttttga cccgttactt
     8161 ctttcaatat gcctttcctt ggaatcaaaa tgaaacgttg gttttttatc ttttcatgat
     8221 cagtaacttt acattagata cacataactc gcatgtccag gagtttttga taagcgaaaa
     8281 atacaaagac gacgtagtag ctcaattgaa tcgaacgttt aaacaatcga tagaagaata
     8341 tttctctttt tatgtttttc cagaagagat ttcgcaaaag attgcgttga cagtgatgca
     8401 aaatcacgct cattttgcct attttagagg atggatgaat atttttggcc aagaaggatt
     8461 aaaacagcgg ttaactgaag tagaagataa acaattcctg gttttatgtg aaagattggt
     8521 cagacaaaca tttgctattg ttgattttac tgaagtagac gaggaaaatg cccgctttga
     8581 attatttgct cgctatgcat ctattttgcg gacgctgatc aagcagttag aattacgtct
     8641 gcaaattgct tatgatcttc cttatgaaag gctaatggcg gatatcgtga aagaaatcat
     8701 ccaggaacaa ttgctgaata cgatcaaata tgatttagtc gaatacgaga aagaaaagca
     8761 gtatgatatc attttgacta gccaattgaa agagtacccg tcacagaagt ccgctaaagt
     8821 ttttgtattc actagcaacg aatataatta tgatttcccc tatttgaacc aatttttaaa
     8881 agaatgttat ttagaaaaat tgaatttaag gatgaataaa acatgaaaat cgtgatcatt
     8941 ggtgcttccc atgcaggcat cacagctgcc ctaaatctaa gaaaacttca accgaaagca
     9001 gaagttttac tgatcgatga agatcataaa gatggactag gctatgtttc aaatggaatc
     9061 aacctttatt taaaaggaaa gatacgtagt ttagctgaag ttgcccataa catgcggact
     9121 cttcaagaaa gtggggcaaa actgatcacc gaatggagag taactgaact tgatccagac
     9181 aaacatcagc tcatcctcgc atccaaagaa ggaaaaaacg agaccataac ctatgataaa
     9241 ttaatcgtag caacaggttc ctctcctgtc accttgtata aacaaattga agctgaaaat
     9301 gtctacacct ataaaaatct agtgcaatct aagcaagtac ttgccgcttt aaaagaagca
     9361 aaagaagtag tgatcttcgg tgctggttat attggattgg aattagcaga tgctttgaga
     9421 aataaaggat acattattca tctagttgat tatatgccga atgttttatc aagatatttc
     9481 gataaggata tgatcaacag ttttcaaaat cagctgcaaa ctaagcaaat caatttttat
     9541 ccaaatgaat tcttgatcga ttggaaaaaa tcagaagaga aagtagtatc cgtacaactt
     9601 ctatctcaag caataaaggc agatatggtc attttttctg cccaaactcg tcctaatacg
     9661 acattgttaa aagaaaaagt ggctttgtat gaggatgaga ctgtgatagt caatgaatat
     9721 ctgcagacaa gtgatccgga catctatgcg attggagata tcgtcccagt cagttttgac
     9781 aaaaataaac ggcatctctt tttaccttta gttactcgag ctgtacatat ggcaagagca
     9841 gtagcgctaa ctttgagtgg tcagccaacc gcttacgacc tcagacaaaa gataacagca
     9901 gcagtcatta tagactactt tttaggaact gtagggctga cagaagatga agcgccattc
     9961 ttggaacaaa ataccggttc gtgttcagga gaatttgatc tatttccaca atacgatgaa
    10021 gaaaacaaaa cagtaaatgc aaagctgatc taccatccgg atacattaga aataattggc
    10081 ggacagctga taagccaaga attcttatta tcagatttga acctgcttgc ggatatcgtg
    10141 aaacacaaaa ctacgattcc acagctagca gttgaggatt ttggatttct gtctgaacac
    10201 acctctcgtt ttcactacct caatgagtta gcttttaaag tactagctaa gagagcaaat
    10261 agcaatagag tagataaaag actgtgagaa atatcacggt cttttattgt gtggggatat
    10321 aataaatatt aatcaatcga gaataaaaaa ttcatataat gaaatagcgg aagaattttt
    10381 tgagcataat attcgccaaa aatttatcaa gtgataaaat tgttgtataa aatgattatt
    10441 tcaggaggag gagttaaatg tttttagcat taaatgaaat tatgcattca aagttacgtt
    10501 atgcgttagt tgcaggggtg atgtttttga tcgcctacct ggtatttttt ttgactgggt
    10561 tagcttatgg cttagctcaa gataatcgaa cagcagtcga caagtgggag gcagattcga
    10621 ttgtgctttc gaaagatgca aattcaaatc tagggatgtc catgattact aagaagatag
    10681 cagaagaagt cgaaggtggg aaagtggcgt atcttgccca gacacctgga gtcgtgacta
    10741 gtaaagacag tacagaagaa ggaaaaatca atgttagctt cttcggtatc gataaaaatc
    10801 aattcatcat gcccaatctt gttgaaggaa aagcttttga taacgatgat gaggcagtag
    10861 gcgatattag tttgaaagaa gaatatggtc tagccgtagg agatacagtt aagttatctg
    10921 gtagtgacaa gacgtttaaa ctaactggct ttacggatca tgccaaattc aatgtctcac
    10981 cagttctcta tacaaccatc aatgcttacc aagagatccg cttcgaaaaa gaagatacaa
    11041 gtgaaaatgc acggattaac gcaatagttg tcagaggtaa aatcagtaat ttacctgaag
    11101 atttagaaca gatcaaaatt tctaaattca tcaatgaatt acctggatat aatgcgcaag
    11161 tattgacttt tggatttatg atcggatttt tgatcgttat tgcggctatt gtgatcggga
    11221 ttttcattta tgtactgacg atgcaaaaga ttaatatctt tggtgtaatg aaagcacaag
    11281 ggatcacagg aggatttatt gcgcgttctg tagtggcaca aacattcatt ttatcatttg
    11341 ttggtattct tcttggtcta ttaggaacag tgggaacatc ccttgttcta cctgatgcgg
    11401 ttccattcca aagcaattgg ctgttttttg gcgtcattag cttgttgatg ctggtagtag
    11461 ctgtgttagg cgcactattt tctgttcgga caatcgtaaa aatcgatccg ttgaaggcaa
    11521 ttggataaga aggaggaaga gcatgaaagc aatagaattt aaagaagtag aaaaaagttt
    11581 ccaagatggc gaccaagtag ttaaagcatt gaaagaaaca accatgagta tcgataaagg
    11641 ggaatttgtt gcaatcatcg gtccttctgg ttcaggtaaa agtacttttt tgactttggc
    11701 aggcggattg cagacgccta gcagtggctc tgtatttatc aatgatcaag aattcagtaa
    11761 tcaaaaagaa agtgaacgcg ttaaactacg ttttaaagaa attggcttta ttttgcaagc
    11821 ttccaatctg gttccttttc tgacagtaaa aaagcagtta ttattagtag ataagatcaa
    11881 acatgaacat cgtcaaaaag aagcaaaaga attatttgag cagcttggcg tggataagtt
    11941 gatcaacaag tatccagaag aactatctgg tggagaacgg caaagggtcg caatcgctcg
    12001 tgcattgtac aatgatccgt ccattattct agcagatgaa ccaactgcta gtttggattc
    12061 agaaaaggca cgagaagtag tcgatatctt ggcaaaagaa tcaaaagaca aaaataaagc
    12121 aacgatcatg gttactcacg atacacggct gattgacaaa tgcgatagaa tatttatgat
    12181 ggaagatggt gtattgaaag aaaaagaata accaaaaagc aggtggaaaa atggtccatc
    12241 gatacaacaa agatacacag caaaaaattt tgaaagaagc gactgacttg tttatgtcca
    12301 agggatatct cggcacttca acaagagaga tcgcacaaaa agttgggatc acccaaccca
    12361 atctctatca ttatttcggt gataaagaaa aactatatac ggcagtttta gaaaaccact
    12421 taaaagatgt tggaaaagct ttgagagaaa tcgttcaaac aagcgaaacg ggatttcaag
    12481 taaccttgac aaaaatggcg cagtttttga ttgagacaca tctggtcgat ttgtttctga
    12541 tgcttcacga tcttgaaagc aatctttcaa aggaaacaag agatcatctt ttctttctat
    12601 ggaagaaaaa ttatcgtgaa ccttttgaag aaatcttttc agcaaaccaa tctgtgctaa
    12661 gaaatgggat ttcaaaagaa atcgcagcaa ggcatttttt cttagtgtta gcaccttaca
    12721 tcactcggtc gaaaagacct gtggaagaac cgctaaatgt ggcgcaattg atcgatttat
    12781 atttgaatgg tgtattagga gaagcgtaaa gctgttatat agggagaaaa atagttaggt
    12841 gaagaagcaa gatatgacga agatcgttat atcttgtttt ttttatatag gtagtttttc
    12901 ataaatacaa atttgttatt ctctcatgat tatcagggta tatttttatt tattatgtcg
    12961 gtgttccagg aagtgtttta atttaataac gaagaaatta attgtttgta gaatgaagga
    13021 aactatttat tgggtagatg aatcaccacg aatagtcaaa aggagatttc aggatatgcc
    13081 aaaaatgaaa aaaatcattt tagcagtaat gttattttct acggttatat cttttacagc
    13141 cccttttttg cgtacttaag ctgattttaa gttttgaccc ttagtctgga tctacgaggt
    13201 gagagtcaag aatgaaaaaa ataattggag cttttgtatt tgcaatcatt atcattatta
    13261 tctgttttca aatagatgtt tttggtcaaa aaaagatagt tggacaaacg gtcagcagta
    13321 cgaacagtac ggttacaaag acaaaggaat catttgatga tacagaacaa gagacattaa
    13381 tcaaaatcgg aaaaaatgac cgtacactat atacagatga ctcgctaact accccacttg
    13441 caactataaa cggcggagag ctggcggagt ttctttctga aacaaaaaat gcttatcaga
    13501 tcaagacaaa tgatggctat actggatatt tagccctcga agatggaacc aaagtcacaa
    13561 agaatataca aacaaaaccg gaagagctat ctgatgcagt gattgtatta gatcctgggc
    13621 atggcggaaa tgatactggg gcattaagca atgatgaaca aacagaagaa aaagatataa
    13681 cattaagtac agccataaaa gtaaaaaagg cgctagagga tgctggagca acggtttatt
    13741 tgacccatac tacagatgaa ttagtacagc ttggagatat atgcgactac agtgaagaaa
    13801 aaaaggcaga tgtctttatc agtttacacg cagactctac ggaatatgcg aatgaagcaa
    13861 ctggaatcac gacatattat tactatggac aagaggaaac attagcacag acaattgctg
    13921 actccttcac tgatctgcct ctggattcaa gaggaatctc cactgggaac tatcaagttc
    13981 ttcgagaaaa tcttcaacct tctattttaa tcgagatggg ttatatgaat aatgatagtg
    14041 atttaaagga attagtaaca gacagttatc agcagcagat tgctgcaagt ttgacacaag
    14101 cattgaccac ttattttcag taataaataa gcccagccaa acaaaaatag ctcctcatat
    14161 ttttcgtttg gccgggctta ttatcgcaga tgcaagcttt tgaacaccgt ttaaggaaga
    14221 ggttgtgagc ctgccgttta actccgcgtc acaaggagca atttcaaaaa acagcttctc
    14281 atgtttgatg actattgtga cttgtgaccg aggagttggc atgagaacac cgtttattcg
    14341 gaacaaggga ctgtgacatg acttttgtca cagttccatt atttttttag cagcttcaaa
    14401 gctttttctg tattggcaaa atgaagtttg gcatattttt caagcatagg catgccgcct
    14461 ttctccaata gttttgccgc tacttggtca gatttcgttc cagctcctga tggaagggtg
    14521 attccagctt cagctgatgc tttgtgtaac tcttccaaaa agctggcacg actgatgata
    14581 gaagcagcag caacagctaa atgatattgt tctcctttag taacgaagta cagtttttct
    14641 tgtaattgat ttttttctaa acgcgcatat tttttaaaat ttgcttccgg cgtaaactga
    14701 tcgatcaaaa tcgcttctgg tttttctggc tcgatttttt ttagcagcaa atggatcgct
    14761 tgattgtgca aagcgacttt catccgaacg gcattgtatt ttggttggat ctcattgtat
    14821 ttttctggag ataagacaag cagtttatag gggatggttt tttttaagat gtcagcaagc
    14881 tggatgattt ggctatcttt taactctttt gaatctctaa caccaagttt acgtaattct
    14941 gcaagctgat cacgtttcac ataagcagca catactgtta gcggaccaaa atagctgcca
    15001 tttccgacct catcactacc taaaacggat aattgactga tattttttgg caaagaagag
    15061 gctgtttttg ctggcttggc ttgtttatcc tgtgcctccc cccatttatc agcttctatt
    15121 gctgcattcg taccttgaaa catcactttt ccggatgtat aagcggtaat ggtcgtcccg
    15181 tctttttttg cagcaaagct agtataagga atctgtttat ttaacaaata agagcgatag
    15241 tatgctttca tttgactaag ttggtcgttt gttgctttga taatgacgtt gctcataaaa
    15301 ttctcctcgt ttttatacat aaaactatac cgcaactcac gacttttcgt tttctaagag
    15361 attgcgagac atagcatttt tttaaagttt atcatataat agttaagtat gtgaaaaata
    15421 tttgtggaga acggtgaaag aaccaacttc atggtacact aaataaaact ttcatcaaat
    15481 aggaggaaat gattcgtatg gcgcacgaaa aaacacgcta caaagcagtg attgccaacc
    15541 aaacatatac tattatcgga cgggaaacca agcatcatat ggatatcgta acgaagctga
    15601 tcaatgaaca gttagcagaa ttgaaacaat tatcaccaca aatggataat gaacaagcag
    15661 caatcttgat ggctgtcaat gcgctttcgg atcaattgaa gaaacaagaa agaatcttgg
    15721 agctggaaga agaaacagca gaattaaaga aaaaaatgat caaatttaca gagcttgaaa
    15781 atcgtgtgaa acgcattgaa gcaatcgaga acgaagctcg ggaagtattg aaagaaaatg
    15841 gtcaagcaga tcataccatt aaaaatcatg tggaagcaca acagatttta aacgagaaac
    15901 gaaaaggaca aattaaacaa aaatcttcta attaagatcc ttttatcaaa ataagaaaaa
    15961 tttaaaaaag ttagaagaga aaggaacgta gcatgctgag tttgctgatt ttatttttat
    16021 tgctaattgc gtttttttct ggagcacgtc gcgggtttgc tttgcaaatc atattcgcga
    16081 ttggatatgt attgtcattt attgctgccc agcattttta caaaccatta gcaagtcatt
    16141 tggaattata cattccatac ccagctgtca ccccaacatc aaagttagca ttttttgatc
    16201 aggtatttgc ttttcaccta gatgaagctt tttatgcagg aactgctttt ctgattattt
    16261 tgctaattgg ctggctgctt acccgatttg tgggggtttt tgtccatgga ttgacgtatg
    16321 tgccgatctt gagacaggta gactggattg caggaggcat cctgtcacta atcatggctt
    16381 acgttactat atttttgatt ttgcagttac ttgcctttgt tccattagtg tggtccaaaa
    16441 tcaattcggc gagcatagtt tcgctcgttt tatcgttgag agaacgccat ttcttaccaa
    16501 caagatgtat gagctttggg taacgaatat tttgaattaa ggaaaatagt gctggaaaat
    16561 atttcgtttt ttgtacagag tagtttattt aagaaggctg cgtagcgtat gtccggcaca
    16621 gctctttttt catagtgaaa gataaggtga taaaatgaat aaccgaatcc ttgaaacatt
    16681 ggaatttgaa aaagtcaaac agatggtcag acaatttgta gtgactgcac aaggaaaaga
    16741 agaactggca gaattggtac cagtgagtga gaaacaaacg atcacaaact ggcttcaaga
    16801 aactgaggat ggattgaaag tgcaacgtct gcgaggcggg atccctatac ctaaacttga
    16861 aaatatccgt ccccatatga aacggatcga gatcggagca gacttgaatg gagtggagct
    16921 tgcacaagtt tccagagtat tgtctacgac aagcgaattg aatcgtttca ttgatgatct
    16981 ttctgacagt gaaatcgaat ttgcacgttt gtatatgtgg gcagatcagt tagtcacgat
    17041 ccctgttttg agccgccgtt taaaggaagc aatcgatgaa gacggacgcg tgactgatga
    17101 cgcttctcct gagttaaaga gcataagaca gaacattcgg cgaagtgaac aagctgttcg
    17161 tgaacagtta gatgggatcg tccgtgggaa aaatgcaaag tatttaagtg atgcgatcat
    17221 caccatgcga aatgaccgct acgttatccc agtcaaacaa gaataccgcg gtgtatttgg
    17281 cggtgtagtc cacgatcaaa gtgcttcagg acagacttta tttattgaac caaaacaagt
    17341 ggtggatttg aataatcgtt tgcgtcaata ccaaatcgct gaacgaaatg agattcaacg
    17401 tattttaagt gaactttctg ctgaacttgt tccccatcgt caagaaatca tccacaatgc
    17461 ttatgtgatt ggtaaaatgg atctgatgaa tgcaaaagca cgatttggta aagaagtaaa
    17521 agccatcgtg ccgggaatca gtgaagacaa tcatgtcgtc ttgaaacaag caagacatcc
    17581 actgatcgat caggaaaaag tagtttctaa tgacatcaca atcggaaaag actatcaagc
    17641 catcgtcatt acaggaccta acacaggtgg gaaaacgatc acgttgaaga ctttaggtct
    17701 gttacaactg atggggcaag caggattacc gattcctgca ggtgaagaaa gtcaaatcgg
    17761 tatttttgag gaagtatttg cggatatagg agacgaacag tcgattgagc aaagtctatc
    17821 gaccttttct tctcatatga cgaacactgt agatatttta tcaaaagtca atgaaaaaag
    17881 tctggtatta tttgacgagc tcggtgcagg aacagatccg caagaaggtg ctgctctagc
    17941 aatcgcaatc ttagatgatc ttggtaaaaa gtctgcttat gtgatggcaa caacgcatta
    18001 tcctgaattg aaagtttatg gatacaatcg ggcaaatacg attaatgcca gcatggagtt
    18061 cgatgtggat acactaagtc caacgtatcg tttgctgatt ggcgttcctg gacgaagcaa
    18121 tgcgtttgaa atctctagcc gattaggtct tgatacagaa gttattgatg aagcgaagca
    18181 gttgatgaat gatgagagcc aagatttaaa cgaaatgatc acagatttag agaatcgccg
    18241 gaaaatggca gagaccgaat atctggaaat gcgtcatttt gtttctgagg cacaagaact
    18301 tcacgatgat ttgaaagaag cttacagcta tttctttgaa gaacgtgaaa aagaaatgga
    18361 aaaagcaaag aaaaaagcaa acgaagtcgt gtcagaggcg gaagaaaaag cagaaaaaat
    18421 cattgctgat attcgtaaaa tgcaacagca gattgggcaa ggaaatgtga aagagcatca
    18481 gttgatcgat gcaaagaccc aattagcaaa tctacatcaa gaagaaactt tgaagaaaaa
    18541 taaagtattg aagaaggcga aagaacaaaa aacgttgaaa ccgggagacg aagtgttggt
    18601 tacgacttac ggccaaagag ggacgctttt acgtaaaaac gggaatcagt ggcaagtaga
    18661 aatcggtata ttaaaaatga atgtgtccga agacgagctg acgcctgttg ctcctcaaaa
    18721 agagccgaca caacgagtta tccatgctgt acgatctgaa tctagtagtc acgtgccgaa
    18781 tcaattggac cttcgaggaa aacgatacga agaagcgcta agtgaggtag atcaatattt
    18841 ggattctgcc attctagctg gttatcctca agtgactatt gtccacggaa aaggaacagg
    18901 cgcacttcgc aagggaatta ctgattatct taaaaaccat cggagcgtca aaagctttga
    18961 gtttgcaccg gcaaaccaag gcggaaatgg tgcgacaatt gttaaattta agtaaagctt
    19021 actaaaggta agcagatggt tcgctatcca agtatagaaa tgctatacta atcatagatt
    19081 ttaaaaatgg aggtctttga tatgtcacaa gtaattacag ataagacatt taatgaagaa
    19141 acagataaag gacttgtatt gattgatttt tgggcaactt ggtgtggtcc atgtcgtatg
    19201 caagcaccga ttttggatca gttggaacaa gaatatgatg aagaagaatt cagaattgct
    19261 aaaatggatg tggatgaaaa tccagaaact cctcaacaat tcggtatcat gagcattcca
    19321 acattaatgt tgaaaaaaga tggtcaagta gtagaaaaag ctgttggtgt tcattcaaaa
    19381 gaacaactac gtcaaatgat cgatcaatat ttataaaaac ttacagcaaa aataagctga
    19441 gggtgggact gaatccagtt ccgtcctcgg tttttttgcg gttgagttaa ggttattcaa
    19501 attaatattt tttgagtata attacgttgt caggctggat tgtttttttg acagacatgc
    19561 tataataact ttaattgaat atgaagggga gtaccagatg aacgaacgaa tcaaaaacaa
    19621 attagcttta cttcctgatc agcccggctg ttacttgatg aaagataaaa atggaacgat
    19681 catttatgtc ggcaaagcca aaatattaaa aaatcgtgtc cgctcatatt ttactggcag
    19741 ccataatacg aaaacggaac ggcttgttag tgaaatcgaa gactttgaat acatcgttac
    19801 agaatcgaat atcgaagcat tgctcttaga gatcaatctg ataaagaaaa atgatcctaa
    19861 atacaacatt atgttaaagg atgataaaac ctatcctttc cttaagataa cgaatgaaaa
    19921 atatccccgt ttggtgatta ctcgtaaagt actaaaagac aaagcactgt attttggacc
    19981 atatcccgat gtcggtgcgg cgaatgagac aaagaagatc ctcgatcgat tatttcctct
    20041 gagaaaatgc aaacctagtc agacgaagga gccttgtctt tactatcatt tgggacaatg
    20101 tctgtgtcct tattatttcg acgtagatcc gaaagtctat acgaaaatcg ttgatgaagt
    20161 gaaaaagttt ttaaacggag gacacgaaaa aatcgagtca gagatccatc agaaaatgga
    20221 aaaagctgct gaaaacatgg agtttgaaaa agctgcagaa taccgagacc agatccgtgc
    20281 cattgagaca atcatgactc gccaaaaaat gacaaatact gatttactgg atcgagatgt
    20341 atttggttat gcgattgaca aaggatggat gtgtgtccaa gtgtttttcg taagacaagg
    20401 aaagctaatt gaacgagatg tttcaatgtt tcctttctat aacgaagcag aagaagattt
    20461 cttgacttac ctcggtcagt tttatcagga aaatgagcat ttcattccta aagaagtctt
    20521 gattcctgat acgatcgaca agcccagtgt agaagctttg ttagctacga aagtgctgca
    20581 accaaaacga ggagaaaaga aaaaattagt tgaattggcg aataagaatg cctcagttgc
    20641 tttgaatgaa cggtttgatc tgattgccag aaaacaggaa cggacgatcg gagcagttga
    20701 acgtttagga aacgcgatga atatcccgac cccaatacgt atcgagtctt ttgataactc
    20761 taatatcatg gggacgaatc ctgtggctgc gatggtcgta ttcattgatg gaaaaccggc
    20821 aagaaaagaa tatcggaaat ataaaataaa aacagtcaac ggaccggatg attatgcatc
    20881 aatgagagaa gtcatctatc gcagatattc acgagtgatt cgcgaagggt tgcctttacc
    20941 tgatttgatc ttgatcgatg gaggaaaagg acaagtagat gtggcaaaag atgtcttaga
    21001 aaatcaatta ggaatcgata ttcctattgc tggcatggca aaaaacgata aacataaaac
    21061 aaatgagttg ctattcggta tagatttggc tgtagtaccg cttgagcgaa attcaccaga
    21121 gtttttctta ctccagcgaa tccaagatga agtccatcga ttcgcaatca ctttccatcg
    21181 gcagctacgc agtaaaaaca gttttgcttc ccgattagat gaaattgaaa ggttaggacc
    21241 aaaacgaaaa aaaatgctgc ttaaagagtt caaatcgttg aaaaatatca cagcagccac
    21301 gacagaagaa ttacaagaaa tcggactgcc gaaaaatgtc gcacaaaacg tatttgataa
    21361 attacatcag gaatgaggaa agggcacttt acaaagaaag taaagtgtcc tatcatttta
    21421 tgtacaacaa aaagagcgtt tacgttttgt tggtaaataa taaatcgctt acgtctggtg
    21481 tctaaggata tatgtgattt cttgaagaag gagtgaagaa atgttagaag tcaaggatct
    21541 tgttaaaact tttgggaact acacagctgt cgatcatgtc tctttccaaa tccccgatgg
    21601 aaaaatcatg gggctaattg gccaaaacgg ggctgggaag acaacgactt ttcgtttgat
    21661 cttggatttt ttgacacaag accaaggaga agtgttatgg aatggccatc atcttgggga
    21721 gaaagattat gatgtcatcg gttatcttcc ggaggaacgg ggactttatc cgaaagtaac
    21781 gattcaagac caactgcttt attttgcaga acttagaggg aaatcaagaa aagagattga
    21841 accaaaaatc gatttttgga tggaaaaatt tcaggtaaaa gggaaaaaga cggataaagt
    21901 aaaatctttg tctaaaggaa atcagcaaaa agtccagctg atcgccacgt taatccatga
    21961 acctaaattg atcattttgg atgagccatt cagcgggtta gatccggtaa atgctgaatt
    22021 gctaaaagat ggaatattgg aattgaaaaa acatggttca tgtgtcattt tttctagcca
    22081 caatatggat aatgtagaaa aaatctgcga tcatttgatc atgttgcgaa acggaaaaat
    22141 ggttttgaat ggaaaagtac atgagattcg tgaatcattt ggccgtacga agttattctt
    22201 ggaatctggt ttaagccaac aggaagtaga agaaatcgaa ggcgtgaaaa aagtgatcct
    22261 tcatgaagac aatacgttag agattacctt agaagatcct gaagctggga aagaaatctt
    22321 tactcgtgcg actcagttcg gctacattcc tatgtttaac cagcaaccgc caactttgga
    22381 agagattttc aaattgaaag caggtgaaat cgatgcgtaa gttttggatt attaccaaag
    22441 atgtctattt aaaaaatgtt aagtcgattt ctttcttgat catgatatta gtccctttta
    22501 tcatgctggg tatcatttat gtagcaggaa actttgctca acaaaatagt gatgtcaata
    22561 aaatcggcgt attggctgat aatgagcaaa tcagtcagca aatgagccaa gtcaaaacag
    22621 atgattttga gttcaaagca gttgattctg aagaaaaagc acaaaaagag ctttcggaag
    22681 aaaaaatcga tgcgtattta gtagtctcaa ctgagaatga taagataaat gggatgcttt
    22741 atagcgaaaa ttcattagga caaagtacac aactactgat acaacaacaa ctgactggct
    22801 tgcaatcgat gcttcgagca agtcaattag gcatttcacc agaagaagta gctagcttga
    22861 gccaacaagc aagtttgact cgacaaaaag tcaattttga ttcaaaagga aaaatgacag
    22921 ttggagaaga taactcgaca gtccaatatg tcgtaagtta tgtagcaacg attgtcttgt
    22981 tcattattat tttgacgtat gcgcagatca ttgcacaaga gatcgcttct gaaaaaggca
    23041 cgcggatcat ggaagtgatc ctgtccagta cgactgcaca aaagcatttt tacggaaaac
    23101 taacaggtgt tttacttgtt gcattaacgc aaatggcatt atacggtatt atttttggta
    23161 taggatacag tcaattcaaa gatatggaaa tggtcaaaaa tatattagac ggtatctcat
    23221 tggataactt gattggacca ttcttgtggt attctcttct attcatgatc tttggcattt
    23281 tgattttttc tgttttagca gctttatgtg gctctttggt caacaaagca gaagatacag
    23341 caaaagcaat tttgccagtg acttatctat ctctaggcgg atatatgctt ggattgatac
    23401 taggagcttc agatccaaac aatatcgtta ttcggatcac ttcgtatatt ccattcttat
    23461 cttcatatat tatgccggtt cgcttagcaa atgagacagt cggtatgggt ggagcattga
    23521 tttccttgtt ggtacttgta gccgtaacat tcttattgat gatcttgtct gcaaacatgt
    23581 acaaatcgaa tgtactcgtg tatagcgaag gcggattatg gagttcattg aaacaatcga
    23641 tttcaattat gagaaatgaa cgaaaaaaag cataaaatat agaaaaatat agaaggaagc
    23701 tggacattta ttgttcccag cttctttttt gtatagacct atttctattt ttttattata
    23761 ttggatatat atattcattg aaaatagagc ataacaactt tataatgttg agtataccaa
    23821 tttttggagg cgttttaatg gaccgctatt tagatttact ttcagagaag ttccctacaa
    23881 cggaagatgt catcacggaa atcatcaatc tcgaagcaat tttgcagctt ccaaaaggaa
    23941 cagagttatt tttgagcgat atacatggcg aatttcctgc ttttgatcat attctgcgta
    24001 tcggttcagg aaatcttaaa gaaaagatca aagacctttt ctctgaacaa atgacggaaa
    24061 cggagatcag ccaattcact atttttacgg cttatccaga atatgtgttg acgactgatt
    24121 ggtacaaaaa acaagataaa tcccagctga tccatcgttt gatcgatctt ttacggttta
    24181 cgacagtcaa atacactcga tcaaaagttc gaaaaggatt gccgaaagaa tatagctata
    24241 tcattgagga actcttgtat atcgatgatc gtatcaacgg gaaaaaagac tatgtaaaaa
    24301 aaatcattga acaacttgtc ttgatgaagg aagaagaaaa atttttaaca aaacttgcgc
    24361 agacgatcca aaaatctgtc atcgatcatt tgcatatcgt gggtgatatt tttgatcgag
    24421 gaactcaagc agccaaagta atggatcgaa tcatggattt ttcctctgta gatatccagt
    24481 gggggaatca tgatattttg tggattggtg cgtattgcgg ctctgaggca tgtttattga
    24541 cgcttttacg gattgccgca cgctacaact atttatttga attagagaaa gaatatggat
    24601 taaatttacg tccattattt tcttttgccc atcaaaccta tcagaagaat ccagtattta
    24661 cccctaaaaa caggaaagat ctatctgaaa gagagcaaga agaattagaa aagatccatc
    24721 aagcactttc tatcctccaa ttcaaatcag aacatcagtt gttgaatcga cgacctgaat
    24781 ttaaaatgga cgatcgtaaa ttgttggaaa aaatcaacta tgaggaatca acgatcgttt
    24841 tagaaggtca gctctatgag ttgaaaggga cttgttttca aacgatccag ccagatgatc
    24901 ctaaaaaatt cttggaagag gaacaaaaag tgatcgatag tttgatgtat tcattccagc
    24961 actcgttaag aatgcaaaaa cacatgacgt ttttgatcga aaaaggggga atgtatctca
    25021 cgaacaatca gcatgtactg ttccatggct gtatacctgt agatgaaaag gggcagctct
    25081 tagcatttga attagaccaa tcttatcgcg gaaaagaact gctgggattt tttgaaaaac
    25141 agataactga aagcgcaaaa gatttgactg caaatgaaga tttggcgacc gatttgattt
    25201 ggtatgcgtg gagcgggcca ttttcgcctt tattcggcaa gagtaaaatg gcaacttttg
    25261 agcgctattt tctcacagag cctcatacgc acgtcgaaaa aagcaatccc tattatcact
    25321 tgcgagatga agagtggttc agtgaaaaaa ttttggcaga attcgatgca aaagaagaag
    25381 gttcagcaat catcaatgga cacacgccag taaaagtaaa aaaaggagag tcaccgatca
    25441 aagcaaacgg aaaagcattt gttattgacg gaggtctatc caaagcttat caaaaaacaa
    25501 ccgggattgc tggctactca ttactatgca attcttacgg tttccaaatc gtgacacatt
    25561 atccgttctt gcctattgaa caacaatttg caaaaaaatc tgatcaaacg aatgtaaaaa
    25621 aagtgatcga gcagcagctc ccacgcaaac ttaatcggaa tacgacaaaa ggaatggaat
    25681 tacagcaaca gattactcaa ctccaaaggc tgttggatta tcgaaaggac gattgagaaa
    25741 aatgctagcc aacattgctc aatagactaa aaagcgactg caacaaaagg cgtgtcgcag
    25801 tctccttcgg aattagctca aaaaacggta aaatattaga agctattttt ctgtttattt
    25861 ctctaatact caggtcaggg cgctgatttc acgacctctt ccttaaacgg tgttctcctg
    25921 ccaactcctc ggtcacaagt cacaatatcc aacaaacatg agaagctgtt ttttgaaatt
    25981 gctccttgtg actcagagtt gaacggcagg ctcacgacct aatcagaaaa acgattaaaa
    26041 ttcattcaca aagaaatcga ataaacgctg ttcagatgaa tctacacgat gagaaagttc
    26101 cggatgccat tggacaccaa ggattttgtg catattatct ttactttgga ctgcttcaat
    26161 gagtccatcg ttggaaaagg cgatggcttt taacaaagga gaaagatctt tgactgcctg
    26221 atgatgatag gaattcacaa agtatgaatc agggagaagg tcacttaata aactgtccga
    26281 aacgattttg acttcgtgag ttgcaaactg cggggcagtg ggctgttgtt cgtgttttac
    26341 ggaccatttt ggatataatg aaagatcttg ataaagtgtt ccctccaaca cgacattcaa
    26401 tagttgaagt cctcggcaga ctgcaaaaat cggttttctt tgtttcaatg cttccaagat
    26461 caatgcctgt tcgaattggt ctcgttgaat atttgtttcc gtcagttttg gatgagggtc
    26521 ttccatgtaa agttgcggac taacatcctg accgcctgca agaatgagtt tgtcgatttt
    26581 ctcaattagt tgaggcgctg aatctggaga catgactggc agaataagag gtaatccttg
    26641 agcatctaaa actgcatcca caaatccctg cggtgtatag cttacttgat ttccttggaa
    26701 agtatctgtc gctcgaatca gttgattccc ggcaatgccg atgattggtt tcatttttat
    26761 tccccctaaa gtagactaat tgtaacaagg atttaaaaaa atttaaacta tgtaagactt
    26821 gcccctatac ttatctgttc ttgctgtgtt actatctgta aagagaattt tcgtgtgaaa
    26881 ttcatgacga tagttgaaaa tattgcttaa tttgttataa taaaaagtaa gcagaaataa
    26941 aaaggttggg cgtaagcttc aaggatctag ttcttcaagg ggtgagaagg acattttatt
    27001 gaaggggcct gcctatacta agttatcttt agaggtacat actttcaatg aaaaaagaaa
    27061 gcatgtacct cttttttaaa tcgaaaggaa gtgaagcaag tgttagaagc tttggaacaa
    27121 atccgaaaag cagaagaaga aaatgagaag cgtgctcgaa ccctacggca gcagttgaag
    27181 acctatgcag aagaaaaaga gcaagaattg gagataatcc aaacgaacag tcggcaacag
    27241 ctgaaacagt tggtagaaga aaaagaacaa ttcgaaaagc aagaattaga aaaagaaaaa
    27301 atgaaattga aagcagaagc agacaaggtg caagcaactt tgaaagacca atatcaaaag
    27361 catcagagtc aagcaatcgg tgcaatcatt gaaagggtga aagaaactta tggccgtcac
    27421 taagatggaa aaagtgaccc tcatttcaga taaaagaaat caggagagga ttttacaagc
    27481 agtacaagga atccaaaatg ttgaaatccg ggatctgtat caaaatagaa caaacaacca
    27541 gtgggtagaa aaatattttc cagataccac gattattgat aaagaagcaa aattaagcgc
    27601 tttaggcaat cggctgaaag acatccaaga agctatccaa ttcatacaac accacggcga
    27661 tagccgtcaa aagaaactcc atttgaaacg tcaagaactt tccttacaag acttggaagc
    27721 aaattattcc gaagaaaatt tcatgaataa attagaagaa gtccttgatt taaaagaaaa
    27781 gtgggaacag ctaactgaaa aacgtgatcg tttagaagaa gaggaagaat ggcttcttca
    27841 ttggcaacag ctggatattg ctccaagtat gtatgcaagt aagacgacca tttttgagat
    27901 ggggactgtg agtgcagcaa attttgaaag tttcaaggaa gaactaagta agcttgatga
    27961 cgtttatatg gaagaaatag attatacgcc aaaacaagtt tatctttcgt atgttgcttt
    28021 aaaaaaatca gttgctgcta tagaagaaat tgctgggaaa tacgggttca ataaagaaac
    28081 gtatccttat gaacaaaatc ctaaagaaaa attgcaagaa accaaacagc aattatccat
    28141 tgttcacgag cagcaaaaaa agataacgac agcgttaggt cagtgcagcg gctatatcca
    28201 agaatttgaa tgggtagaag aagtcacact tgccgttgca gaacgggaaa aagtaaaaga
    28261 acggtttatc catgccactt atctgatcgt tttgcaaggt tgggtagatg cagaagaaaa
    28321 acagaatttg atgcatgtgt taaacgaaac tgtatcagaa gaagatatat acgttagttt
    28381 cgaaacacca aactcagaag agattcagca ggaagtccct actaaattga aaaaccatcc
    28441 gcttatcgaa ccttttgaat tgctgactga aatgtacagt ctgccaaaat atgaagaagt
    28501 agatccaacg ccttggatga caccatttta tcttgtcttc ttcgggatga tggtcgcaga
    28561 tgttggttac ggattgctga tgctcattgg gacaatacta gctcaaaaac tatttgtttt
    28621 accccgcggg atgaaacgtt tcgtaaaatt ctttgagatt ctctcggtac catcgatcat
    28681 ctggggattg atctacagtt catttttcgg acaggcatta ccaaaagaac tatttggtat
    28741 ccgcttgcct ttcccaatcc tatctacgac ggatgacgtc aatacgattt tgattttatc
    28801 cgtgattttc ggtctgatac agatcttggt tggattattc gtggcagcaa aggaaaatat
    28861 caggaggaaa gattacttga gtgctgtcag tgatggtttt gcctggcaag ggatcctgat
    28921 tgggatcgtt attgcattga ttggtgtcat gttgataaaa aatacagcat ttgtttatct
    28981 tggcgcgggg ctatcgatca ttgctgcttt atgtatctta atcgtgccga ttatccaatc
    29041 gccgtctaaa gtcaagggtg ctgccaaagg agcgtataat ctttacggat tgactggtta
    29101 tatcggtgat ttagtcagtt atacacgttt gatggcacta ggtatttcag gcggaagtat
    29161 tggcgcggca ttcaatatgc tagttgcttt catgccgcca gccgctcgat tcagtgtagg
    29221 gatccttttg atcatcgctt tacacgcact gaatatgttt ttgacactgt taagtgccta
    29281 tgtccatggt gcacgattgc agtatgtcga attttttgga aaattttata caggaggcgg
    29341 acgtgccttt gacccgctga aaacggcgga aaaatacgtc aacatcaatc ataagaaaaa
    29401 aaacaataaa taattggagg atttttatac aatgatggat tacttaatta gtcaaaatgg
    29461 tggaatggta tttgcagtat tagcgatggc aacagcgacg attttttcag ggattggttc
    29521 agctaaaggt gttggaatga caggtgaggc agcagcagcg ttaacaacaa gtcaacccga
    29581 aaaattcgga caggctttga ttctgcaatt gcttccaggt acgcaagggt tgtatggatt
    29641 cgttatcgcc tttttgattt ttatcaactt aggaaatgat atgtcagttg tacaaggatt
    29701 gaactttcta ggcgcttctc tgccgattgc ttttacaggt ttgttttcag gtatcgccca
    29761 agggaaagtt gcagcagcag gtatccaaat tttagcgaaa aaacctgaac atgctacaaa
    29821 aggaatcatt tttgctgcga tggttgaaac atatgcgatt ttaggtttcg ttatttcctt
    29881 cctattagta ttgaatgcct aagcataaag gaggaaaagc agatgaacgc cattgaaaaa
    29941 atcatctctc agatgaatga agcagcagag caagaaagag cggcgttaga gcaagaagag
    30001 cgaatgaaga tcgatcaaaa ctttgaacaa aaacgtacgc aaatcgaaac agaacatcaa
    30061 aaacaaaaag aaaaacaaat cgaattactt gaaaaaaaat accgtcagct taggaatcgc
    30121 caacaagtag aagtacgaca agaaaatttg aatgcgaagc aggaattttt acgacgattg
    30181 tttgcagatg caatagctga aatggagaat tgggatgaat ctgaacaaat ccagtttatc
    30241 aaaaattcat tgtattcatt acctttgaca ggcaaagtaa cattcattgc tggagaaaaa
    30301 tcagcagctt atctttctca gacattgctg gatgaatgga acaatgaact tccctttatg
    30361 atggttttaa gtgatgaaac tgttgctgat caagcgggtt ttctgataaa cgatcaagga
    30421 gttcagtata attttctttt cagcagcctt gtacaagata tccaaggaac gatgagtttt
    30481 gagattgcca atcaattatt cgaataagga gggatcagtg tatggaatac cacgaactaa
    30541 atcccttgat tcgcggaaga gagcttgaac ttttgtccaa tgagacattt gaacggatga
    30601 ttcaaacaaa ttctgtcgaa gcacttggtg aaatattgaa atccacgatt tacagtccat
    30661 atatttatga cggatatgaa tatgacttcg aacagaatct tttaaaggag cagagccgtc
    30721 tatttcgctg gttaaaggaa tcagcaccgg agcctgaaat cgtttggatc tatacgatgc
    30781 gctatacttt ccataatctg aaggttttga caaaagccga gatgacaaat caaaatctgg
    30841 accatttgta tatcgacgat ggattttatt cattagaaac cttgaaggat gccattcata
    30901 cgcaagtttc aaatgaactc cctgaaagca ttatggacag tatacgagaa gtacgtgatt
    30961 attttgaaga gtctaccatt cttcagggaa ttgacgtgat ttatgatcga cagtttttga
    31021 cagagcagcg acggcttgcc gataagctag gatatcaaga attgctgaca gaagtcattg
    31081 cttttattga cttgaccaat atcacaacga tggctcgagg aatattgcag aaaagaacat
    31141 atgggttcat gacgacggta ctttccagct cgggaagtat cgaaaaagaa gtatttttga
    31201 actttgtaga taaagatatg gaagattaca cgaattttct attagagaca gattatgctg
    31261 attttctgcg ccctgtcatc cgtgagggaa atcttgatct cgtaaagctt gaacagttga
    31321 aagatgatta tctaagttca ttgtatcaag aagcacaaac ccaggctttc ggaccactgc
    31381 cactactagc ctttttgaac gcaaaagagg tagaaagcaa aaaccttcgt ttactggtta
    31441 tcggcaaaag aagccatttc gaaccagaaa caatcagaaa gcgggtgaga caagtctatg
    31501 acgcataaga tcggtgtagt tggcgacaaa gattctgttt tgccattcaa attgtttggc
    31561 tttgatgttc gttatggtac aaccaagcaa accgttcgtc aggcaattga agaaatggca
    31621 aagaatgagt atggcgtgat ttatgtcaca gagcaatgtg cagcattgag cccagaaacg
    31681 atcgaacgtt acaaggaaca aatgatacct gctatcgtat tgattcccaa tcatcaagga
    31741 tcattaggga tagggctgga agaaattcaa aaaaacgtag aaaaagcagt aggtcaaaat
    31801 atcttatagt atgaaaagga gatagaaatt ttgcaaattg gaaaaatcat aaaagtctcc
    31861 ggacctcttg taatggctga gaacatgtct gatgccagta tccaagacat gtgtctagtg
    31921 ggagatctag gagttatcgg agaaattatc gagatgcgtg gcgatgtggc gtctattcaa
    31981 gtatatgaag agacttctgg aattggtcca ggcgaaccgg ttcgctctac tggtgaagct
    32041 ttatctgttg aattaggacc agggatcatt tcacaaatgt ttgacggaat ccaacggcca
    32101 ttagatacct ttatggaaat cacacaaagt aatttcttag ggcgcggagt tcaattgccc
    32161 gcattggacc atgagaaaaa atggtggttc gaaccaacag tagaagccgg agaaacggtg
    32221 agtgctggag atgttatcgg gatcgtagaa gaaacaaaag ttataaaaca taaaatcatg
    32281 gtgccaaatg ggataaaagg aacgatcaaa acaatcgaaa aaggttcatt cactattgat
    32341 gaaacagtat gtgtcattga aaccgaaaat ggtgacaaaa atttgacgat gcttcaaaaa
    32401 tggccagtaa gacgtagtcg gccaatcaaa gaaaaattaa acccggatgc acccatgatc
    32461 actggacagc gagtgattga tacattcttc ccagtgacaa aaggaggagc agcagctgta
    32521 cctggtcctt ttggtgcagg gaaaacagtc gttcagcatc aaattgcaaa atgggccgat
    32581 gtggacatgg ttgtctatgt gggatgtggc gagcgtggaa atgagatgac ggatgtttta
    32641 aatgaattcc ctgaattgat tgaccctaat acaggagaat cgttgatgga acgtacggta
    32701 ttgattgcta atacatcaaa catgccagta gcagctcgag aagcttctat ctatacggga
    32761 atcacaattg cggaatattt ccgtgacatg ggttatgacg ttgctatcat ggcagactcc
    32821 acttcacgat gggcagaagc acttcgtgag atgagcggac gtttggaaga aatgcctggt
    32881 gatgaaggtt atccagctta tcttggttca cggcttgcgg aatactatga acgatctgga
    32941 agagtcattg cattaggatc agaaggtcgc gaaggaagta taaccgcaat cagtgctgta
    33001 tccccgtctg gaggggatat ttcggaacca gtcacacaaa atacattgcg ggtcgtaaaa
    33061 gtattctggg gtcttgattc cagtttagcc caaaaaagac attttccttc gatcgattgg
    33121 atacaaagct attctcttta ttcaacagaa gtagggaagt atatggatca tatccttcaa
    33181 gctgactggg catccatggt caccgagggc atgcgtattt tgcaggaaga acagcagctg
    33241 aatgaaatcg tgcgtttggt agggattgat tccctttcag ataatgaccg tctgacatta
    33301 gaagtggcaa aatccattcg tgaagattat ttgcagcaaa atgcttttga tgatgtcgat
    33361 acattcactt caagagagaa acaattcaaa atgttgaaga tgatcttgac ctttggtgaa
    33421 gaaggacgcc gtgcgttgtc gttaggtgct tatttaagtg agatcatgga cggtaccgta
    33481 tcgattcgtg aaaggatcgg acgaagcaaa tatatcccgg aaacagaatt agaaaaacta
    33541 gatcaaatta ttggtgaaat caagcaaacg atgcaaacga tcgtttcaga aggaggcatg
    33601 agcaatgatt aaagaatatc gtacgatcgg tgaagtcgtc ggtccattga tggcggttga
    33661 aaaagttgct ggtgtcaaat atgaagaatt aatcgaagtc cgtatgcaaa atggcgagat
    33721 tcgtcgtgga caagtattgg aagtccaaga agataaagca atggtccaaa tattcgaagg
    33781 aacaagtggt atcaatttac gtgactcttc tgtacgattt ttaggccatc ctttagaact
    33841 aggtgtatcg gaagacatga tcggtcgagt attcgatggt ttgggacgac cgaaagataa
    33901 cggaccagag atcctgcctg aaaagcattt ggatattaac ggagaagtaa tcaatccagt
    33961 agctcgggat tatccagatg agtttatcca aacaggtatc tctgcaatcg atcatttgaa
    34021 tacattagtt cgtgggcaaa aattaccagt attttctggc tctggtcttc ctcataaaga
    34081 attagctgct cagatcgcgc gacaagcaac ggtactcgat tcatcagatg atttcgccgt
    34141 agtctttgct gctattggaa tcacttttga agaagctgaa tttttcatgg aagattttcg
    34201 tcaaacgggc gcaatcgacc gttctgtcat gtttatgaat ttagcaaacg accctgcaat
    34261 cgaacgtatc gctactcctc gtatggcttt gactgctgca gaatatcttg cttatgaaaa
    34321 gggaatgcat gttttggtta tcatgacaga catgaccaac tatgcagagg cattgcgaga
    34381 gatttctgca gcacggcgag aagttccagg aagaagagga tatcctgggt atctttacac
    34441 gaacttagct actttattcg aacgagcagg aagaatccgc ggactaaaag gatcggttac
    34501 tcaaattccg attctaacaa tgccagaaga cgataaaact catccaatcc ctgacttgac
    34561 tggttatatt acagaaggac agatcatctt gactagagag ctttacaaga gtgggattca
    34621 gccaccaatt gatgtactcc cgtctctttc gcggttaaaa gataagggaa ctggacccgg
    34681 aaagacaaga gaagaccatg cagctacgat gaatcagctt tttgcagcct atgcacaagg
    34741 aaaacaggca aaagaattag ccgtagtttt aggtgaatct gctttatctg atgtagacaa
    34801 gatctatgcg aagtttgctg agcggtttga aaaagaatat gtcaatcaag gattctacac
    34861 gaaccgttcg attactgaaa cacttgatct agggtgggaa ctgctatcga tgttgccaag
    34921 aaccgaatta aaacgaatca aagacgacat gctggacaaa tatctgccag aagggaagtg
    34981 agttctgatg gcacgattga atgtgaatcc gacccgcatg gaattgactc gattgaaaaa
    35041 acagttgaca actgccacaa gagggcataa attattaaaa gataagcaag atgaactgat
    35101 gcgtcaattc atcctgttga ttcgaaagaa caaccagctg cgtcaagaaa tggaagagtc
    35161 tatgacagaa gcaatgtctg attttgtatt ggcaaatgct tcgatcacgg aacctttcat
    35221 cgaagaatta tttgttttgc cggcggaaaa cgttgatttg tctatcgtag acaaaaatat
    35281 catgagtgta aaagtacctg tgatgaattt tgaatatgat gaacagctga cagacacacc
    35341 tttggaatac gggtatttga attccagtgc cgaactggat cggtcaatcg atcggtttac
    35401 taccctgctt ccaaaacttt tggaactgac agaaatcgaa aaaacttgtc aactgatggc
    35461 gagtgaaatc gaaaagacac gtcggcgagt caatgcatta gaatatatga cgatccctca
    35521 attagaagaa acgatctatt atattcgtat gaagctggaa gaaaacgagc gcgcagaagt
    35581 aacaaggttg atcaaagtaa aaaatatggg taccactgac taagtggtat ctttttttaa
    35641 aagaggaaaa cgatgattaa acaaagcaaa caaataaaga aacgtttgcc ggctgttcag
    35701 cttctcgcat taggattttt tctcctgatt ttagttggcg gcggcctact taccctacca
    35761 ttttttagta atagtgggca tgggacaaat tttatcgatg cgcttttcac tgcaacttct
    35821 gctgtctgtg taacgggatt gacaacattg aatacagcag agcattggaa cagtgctggt
    35881 caatttttga tcatgctttt gattgagatc ggcggtcttg gttttatgat ggttccgatt
    35941 ctatgctttg cattgataaa gaagaaagtc agtctcagca cacgtattat attaaaagaa
    36001 gcgctgaatc tagaagaagt atcaggtgtg atgaagctca tgctttacat cctaaaattg
    36061 gcctttgtta tccaattgat tggtacggtt gctttatctt ttgtatttgt gcctgaattt
    36121 ggctggttga aaggtatctg gtacagtttg ttccatgcaa tctctagttt ttgtaatgca
    36181 gggtttgatt tgttaggaga cagtttagcc aatcaccagc aaaatgttta tttgatcatg
    36241 gtggtatctg ctttgatcat ttctggtggg ttaggcttca tcgtatggcg tgatttactg
    36301 gaatatcatc agaaaaagaa gatgacgatt cattcaaaaa tcgcattaac cgttacaggt
    36361 acccttttat taggcggatt cgttgtgttc tttttcacag agcgcaatgg ggcaacgcta
    36421 gtggaaggga atatgtttca acgattggca aacacctttt ttatgagtgt gacacctaga
    36481 acggctggat attattcagt tgattacctc catatgagcc atgcaggtct gattttaacg
    36541 atgtttttga tgtacatcgg cggaacatct gggtcgacgg ccggcggatt gaaaacaact
    36601 acgttaggtg tgttgctgat ccagatgcgt tcgatgttga aaggacgtac gagagcggaa
    36661 gcatttggcc gtaccatccg acaaggcgca gtgttgcggg cacttacttt attttttgtt
    36721 acgttgacgt tgtgtgtagt cgccatcatg attttatcgg tgactgaaac gatacctaaa
    36781 acttctggca tcgaatatat tgcttttgag gtcttctctg cgtttggtac agtaggtctt
    36841 acgatgggat tgacgccaga cttgactgca atagggaaat tgatcattat agcgcttatg
    36901 tacattggac gagttggtgt attgactgtg gtcttctctc tgatgacaaa agcacaaaga
    36961 acacaatcaa aatacaaata tccagaagaa agtgtcatga ttggataatt tcatcttctg
    37021 acagaaacct agtgatttag taaaataaga aaataaaata atttttcttg cttcttggat
    37081 actttaagtg caaaatgttc gtttattttt tgaggcacta cccgttgttg aacctcgggt
    37141 agtgcctctt ttttattgaa aggaatcaat aagatttgat tcctttattt atcaataggt
    37201 tataattcat att
//
LOCUS       DARDHA010000012        32892 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000012 DARDHA010000000
VERSION     DARDHA010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.012"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            352..1041
                     /locus_tag="SDA10_000503"
     CDS             352..1041
                     /locus_tag="SDA10_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4239433.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1028..2236
                     /locus_tag="SDA10_000504"
     CDS             1028..2236
                     /locus_tag="SDA10_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG4239434.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2530..3801
                     /gene="serS"
                     /locus_tag="SDA10_000505"
     CDS             2530..3801
                     /gene="serS"
                     /locus_tag="SDA10_000505"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEG4239435.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="SDA10_000506"
     CDS             complement(4350..5834)
                     /gene="guaB"
                     /locus_tag="SDA10_000506"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEG4239436.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6339..7034)
                     /locus_tag="SDA10_000507"
     CDS             complement(6339..7034)
                     /locus_tag="SDA10_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEG4239437.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="SDA10_000508"
     CDS             complement(7124..8224)
                     /gene="ychF"
                     /locus_tag="SDA10_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEG4239438.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8233..8424)
                     /locus_tag="SDA10_000509"
     CDS             complement(8233..8424)
                     /locus_tag="SDA10_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEG4239439.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8445..9335)
                     /locus_tag="SDA10_000510"
     CDS             complement(8445..9335)
                     /locus_tag="SDA10_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEG4239440.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9322..10089)
                     /locus_tag="SDA10_000511"
     CDS             complement(9322..10089)
                     /locus_tag="SDA10_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG4239441.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="SDA10_000512"
     CDS             complement(10101..10814)
                     /gene="rsmG"
                     /locus_tag="SDA10_000512"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEG4239442.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="SDA10_000513"
     CDS             complement(11046..12947)
                     /gene="mnmG"
                     /locus_tag="SDA10_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEG4239443.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="SDA10_000514"
     CDS             complement(12964..14361)
                     /gene="mnmE"
                     /locus_tag="SDA10_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEG4239444.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14660..15043
                     /locus_tag="SDA10_000515"
     CDS             14660..15043
                     /locus_tag="SDA10_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239445.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15211..15969)
                     /locus_tag="SDA10_000516"
     CDS             complement(15211..15969)
                     /locus_tag="SDA10_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEG4239446.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(15987..16805)
                     /locus_tag="SDA10_000517"
     CDS             complement(15987..16805)
                     /locus_tag="SDA10_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEG4239447.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="SDA10_000518"
     CDS             complement(16802..17158)
                     /gene="rnpA"
                     /locus_tag="SDA10_000518"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEG4239448.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="SDA10_000519"
     CDS             complement(17276..17410)
                     /gene="rpmH"
                     /locus_tag="SDA10_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEG4239449.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17933..19267
                     /gene="dnaA"
                     /locus_tag="SDA10_000520"
     CDS             17933..19267
                     /gene="dnaA"
                     /locus_tag="SDA10_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEG4239450.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19465..20595
                     /gene="dnaN"
                     /locus_tag="SDA10_000521"
     CDS             19465..20595
                     /gene="dnaN"
                     /locus_tag="SDA10_000521"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEG4239451.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20824..21066
                     /gene="yaaA"
                     /locus_tag="SDA10_000522"
     CDS             20824..21066
                     /gene="yaaA"
                     /locus_tag="SDA10_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEG4239452.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21053..22177
                     /gene="recF"
                     /locus_tag="SDA10_000523"
     CDS             21053..22177
                     /gene="recF"
                     /locus_tag="SDA10_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEG4239453.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22174..24120
                     /gene="gyrB"
                     /locus_tag="SDA10_000524"
     CDS             22174..24120
                     /gene="gyrB"
                     /locus_tag="SDA10_000524"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEG4239454.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24140..26611
                     /gene="gyrA"
                     /locus_tag="SDA10_000525"
     CDS             24140..26611
                     /gene="gyrA"
                     /locus_tag="SDA10_000525"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEG4239455.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26847..27146
                     /locus_tag="SDA10_000526"
     CDS             26847..27146
                     /locus_tag="SDA10_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEG4239456.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27196..27708
                     /gene="ssb"
                     /locus_tag="SDA10_000527"
     CDS             27196..27708
                     /gene="ssb"
                     /locus_tag="SDA10_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG4239457.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27734..27970
                     /locus_tag="SDA10_000528"
     CDS             27734..27970
                     /locus_tag="SDA10_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEG4239458.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28113..30086
                     /locus_tag="SDA10_000529"
     CDS             28113..30086
                     /locus_tag="SDA10_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEG4239459.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30092..30544
                     /gene="rplI"
                     /locus_tag="SDA10_000530"
     CDS             30092..30544
                     /gene="rplI"
                     /locus_tag="SDA10_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEG4239460.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30818..32185
                     /gene="dnaB"
                     /locus_tag="SDA10_000531"
     CDS             30818..32185
                     /gene="dnaB"
                     /locus_tag="SDA10_000531"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEG4239461.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
BASE COUNT        10005 a         6426 c         6475 g         9986 t
ORIGIN      
        1 tttccaacat gtcaacaact tattttttat tatttaataa gttattggtg ttcttttgaa
       61 caaataataa tatatcaaca tcacatgtga aagtcaataa gaaaatgctt atttatcaac
      121 atttctagct tacatgatca ttggttagtt ctggtccttt ttcgttcgtc tttataactc
      181 cgagagacgt aaaatccttt tgttttcagt ttaatgttca tgttcttgtc ttattcacac
      241 caactagatg tctcaccttc taaaattttc cttaacaaag tgctgctttt atctatttct
      301 gctttttttt gctagaataa tagtaacaat atgactctgg aggatttatt tatgaaaatt
      361 ttgattgctg acgatgataa agaaatcgtt gaattattaa gtatctatgt tcacaatgag
      421 gggtatgaag cagtaaaggc ttatgatggg aaagaagcat tatctaaaat cagaacaatc
      481 cctgatattg aattacttat tttagatatc atgatgcctg agatggatgg gatgcaagta
      541 gtgaaagagc tacgaaaaga atcccaaata ccaatcatta tgctgacagc aaaaactact
      601 gatatggata agatcaaagg actggtcgct ggtgcagatg attatgtgac caagccattt
      661 aatccgttag aagtaatggc tagagtaaag tctttattac ggcgtacaaa aatgcagatt
      721 gccccagatc agcctgatgt attagaagta aatgcattaa tcatcaataa agactctcat
      781 gaagttaaaa caatcgatgg gaaagaaatc cagttaacag ctttggaatt cggtatcctc
      841 tacttgcttg ctagtcatcc taatcgagta tttagcgcgg atgaaatttt tgagcgtgtt
      901 tggaaacaag aaagtattgt ctctgctaaa actgtcatgg ttcacgttag tcatttaaga
      961 gataagattg aagaagcaac tggtggtgaa aaagtcattc aaaccgtttg gggagtgggc
     1021 tataaaattg acgcaagata gtaaacgagc aactgaaaaa cgccgtcgga tcaatttgac
     1081 ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc acgattatcc ttttgcttct
     1141 attaaatgtt tctattctgg ttgttatcag ttcggtgatc aacagttcac cttctttgac
     1201 gaatgcgatt tgggattcaa agaatatttt tgcagaacga ttgaacactg atttgttctg
     1261 gaacggccgg aattttatta ttcccttttt ctttctatta gatattggtg ttttgtattg
     1321 gcgcttgatt cgaagatatc gccaaatgca attgcggcat atcatcagcg aacttcacta
     1381 catcgcaaac ggaaattatg atcatcgtat cccttttgaa ctgagtggtg acttaagccg
     1441 agtggtaaca agtatcaatg gattggtgga tagtaccgta gcagcgattg aagacgaacg
     1501 taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc agtcatgata tccgtacccc
     1561 actcacgtcc atcattggat acctaggact gatagaagat ggccagtatc actcagaaga
     1621 agatctttta aagtatacgc atactgctta tatcaaagca aagcagatga aatctttagt
     1681 ggacgactta tttgaataca ctaaggtacg ccagcctgct gtcccggtga atttttctgc
     1741 ctttgacatg atccagttga tcgaacagtt agcagctgat tttgagttgg aagcttctaa
     1801 aaaaaatatc caaatacttg ttcaatcaaa agtagactcc cttatcatgg atggcgatac
     1861 cgaaaaactt gtacgtgtct tcaataatct tttgacgaat gcgttgaaat acggaaaagg
     1921 agcaacgaag atcgtaatcg aagtagaacg tatcggttca gaagttgttg caacagtcaa
     1981 aaataacgga gcaatgatcc cacaacaggc aatcgataat ctattcgacc gtttttatcg
     2041 tgtagaagaa tctcgttcac aagcaactgg aggaactgga cttggtttag caatcgctca
     2101 aagcattgtg gctttgcacg gcggatacat ttatgcaaaa tcagataagc aatggacttc
     2161 tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta cctattgata cacaggaagt
     2221 tattgacgaa agctgaaata ttcgctattc ttattacaat tattagaata aatgaaaaac
     2281 acagaaccca gtacacttta ttctttttca gagagctgat ggcaggtgta aatcagcaaa
     2341 ggataatttg gaagttccag ttcgtttacg tgccctttcg aaataagaaa ggtcggttca
     2401 acagccgtta tgtgttcaac aagtgcagct gacgctgaac ttgggtggta ccgcgaattt
     2461 cagactgatt cgtcccaaga ataatgggat ggatcagtct ttttttattt tcaaaaagga
     2521 ggaaatcata tgttagatgt aaaaatgatg cgacaaaata ttgaagaagt acaagaaaaa
     2581 ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat tcatggaact agatgaaaat
     2641 cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat atcgaaatga tgtttctgct
     2701 gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg ctaaaatcgc tgaaatgaaa
     2761 gaagtcggtg gcaacatcaa agcattggat actgaaattg ctgaagtgga tgaaaaattg
     2821 aaacaaattg caactacttt gccaaacctt ccccatgatt ctgtgccagt tggaacagat
     2881 gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac gtacatttgc ttttgaacca
     2941 aaaccacact gggaaattgc agaaaatctt gatattttag attttgaacg tggcgcaaaa
     3001 gtagccggta gccgttttgt ctattacaaa ggattaggtg ctcgattaga acgtgctgtc
     3061 tacaacttca tgcttgatca gcatatttac gaacacggct atactgaaat gatcactcct
     3121 tatatcgtca acagtaaagc gatgtttggt acgggacaat ttccaaaatt caaagaagat
     3181 gttttccaat tggagaatac tgatctaaca ttaatcccaa cagctgaagt accattaact
     3241 aactattata atgatgaaat tttagatggt gcagatttac ctatttattt cactgcacta
     3301 agccctgctt ttcgttctga agccggaagt gctggtcggg atacacgagg actgattcgt
     3361 cttcaccaat tcaataaagt agaaatggtc aaattttcgg atgtagagca ttcttacgaa
     3421 gaattggaaa aaatgacagc tgatgcagaa gatatcttga aaaaattgca tttgccttat
     3481 cgtgtgatgg ccttatcaac tggagacatg ggcttttctg cagctaaaac ttatgactta
     3541 gaagtatgga tacctgcaca agatacttat cgtgaaatca gttcttgttc taactgtgaa
     3601 gatttccaag cacgccgcgc aatgatccgt tatcgcgata atgatggaaa aatccactat
     3661 actcatactc tgaatggttc aggacttgca gttggccgta cagtagctgc cattttggaa
     3721 aactaccaaa atgaagatgg ttctgtcaca gttccagaag cgctcgttcc ttatatgggg
     3781 aatctgaaag ttattaaata aagaggctgt gaaaaagccg cttagtttcg agtattaaga
     3841 aaaattttgt gaaaatggct ttccacattt ttgcataaat tggacttaat accgagaaac
     3901 tggcttttga acaccattta tcgagaggtt gtgaaatcag cgaattaacc tgagtattag
     3961 agaaaaaaat ggaaaaatag tccctcatat tttaccgttt gttgaactaa taccgagggt
     4021 cagctgattg aacaccgttt agtggagagg tcgtaagcct accccccagc tcctagttac
     4081 aaggacaaat tcaaaaaaca gctcttcata ttttatgaat gttgtacttg tgattcggag
     4141 gtggcaaggg aacactgttt attcaaaaag aggctgggac agaagcgttt aactccaaga
     4201 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttctt cttatttccg
     4261 aaggagttgc ttctacttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct
     4321 ttacttttgt cccacgctct ttaatgtttt taagattcaa ctgaatagtt tggtgcttct
     4381 tttgtgattt gtacatcatg agggtgtgat tcttttaatc cactgccgct catttggata
     4441 aattgcgctt catcacgtaa ttgctgtaga ttggctgccc caacataacc cattcctgaa
     4501 cgcaaaccgc cgatcatttg gaaaataata tctgcaacac ttcctttata agcaacccga
     4561 ccttcgattc cttctggtac taatttattt gcttcattga cactaccttg gaagtaacgg
     4621 tcttttgaac ctttttccat cgcgcctaat gagcccatac ctcggtatgt tttgaatcga
     4681 cgtccttgga agatttcgaa ttcacctggt gattcatctg tccctgccaa catacttcct
     4741 agcatcactg catgtccgcc ggcagctaat gctttgacga tgtcaccaga atatttgatc
     4801 cctccgtctg caataatcgc tttgccatat tgacgagcaa ctgatgctgc atcataaata
     4861 gctgtgagct gaggtacacc tacaccggct accacacgtg tcgtacaaat cgatcctggt
     4921 ccgataccaa cttttacgac atctacacct gcatcatata aagcttttgt cgcttcagcc
     4981 gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag ttgaacggat ttcttgaatt
     5041 tttcggatca cacctgcact atgtccatga gctgtatcga tgatgattgc atctgcccct
     5101 gcttctaata aagcattcgc acgctcaaat gtatcgcttg ttacaccaac ggctgctgct
     5161 acaagcaatc gaccatgttc atcttttgct gcatttggaa attcgatcac tttctcgatg
     5221 tctttgatcg tgatcaatcc gcttaaacga ccttcgttat ctacgattgg taatttttcg
     5281 attttatgtt tttgcaaaat cttttctgca tcttttagag aggtacctac tggggcagtc
     5341 actaagttgt cttttgtcat cacatcagca atcggcatgt gataatctgt tacaaaacgc
     5401 atgtcgcggt ttgtaattat ccctaccaat tttctgtttt caagtgtttc aacgattggc
     5461 acaccactga tacgatattt gctcatcaat tcttctgcgt ctgctacaag atgctgtggc
     5521 gttaagaaga acggatcaat gattacgcca ctttcagaac gtttgacttt tcttacttca
     5581 tccgcttgct ggctgatcgt catgttttta tggatcacac ctagtccgcc ttgtcttgcc
     5641 atagcaatgg ccatcttgct gtcggtaaca gtatccatac ttgcactgat gattggaata
     5701 ttcaacttaa tatttttagc taattgtacg ctcatatcca cttcgtttgg taacacgtga
     5761 ctttctgctg gtatcaataa gacatcatca aaagtaaatc cttttttcgt gaactttgtt
     5821 tcccagttgg acatttttag taccactcct ctaatttagt ttatctatta aaataattga
     5881 ttttttcatt taagtcaatg atctacttgg aatcaagtta gctttctcat agaataccaa
     5941 tataaagcgt ttacatctgt gtatattaaa agagattgta aaaaagctgt cctgcatcaa
     6001 gtaataagaa cagccaatca aaaacagctt ctcatgtttt tgaattggtt gggtttatta
     6061 ccgcagatgc aaacttttga acaccgttta gtggagaggc tgtgaaaaag ctgcttagtt
     6121 tcgagtagta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aattggactt
     6181 aataccgaga aactggcttt tgaacaccgt ttatcgtaaa aagggactgt gacaagactt
     6241 ttgtcacagt cccttactat tatttaacta tggataaaca aaatgaggtt ctttactacc
     6301 cctagatatc tagcaaaata gctgatcttt ttgctagttt atcttcttgg gacggataaa
     6361 cttccaataa tcccgtattt tttcttcaag tagtaacgga ttgctaaagc agttgccccg
     6421 ataatgataa ctactactgg atctaatact ggattgataa ctggtggcaa tagtgttgtc
     6481 gcagaaaaca gtacgaccca aagaagtgtc gttccaccta ggataagcaa tgtcttaccc
     6541 attcctggtt tcttgcttcg atctgctccg ggatattcat attgatagat gtatttgtac
     6601 atcatataaa agacccagcc accgattgca gaacctagaa cgagtgtaga aaagccataa
     6661 gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa tcatgatact taatagacca
     6721 agcaacagca acgtattatc taaccacatc aataaagggg tggatgaatt ttttacttct
     6781 gctggcttat tgatgattgc ttccgttctt tcagagactg tcccaaacaa ttgacgggct
     6841 gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa tgtcatgaag ggcttttact
     6901 ttttcttctt cagacaaatt cgctgcttct aaagattttt tcaaatcaaa aatgtactgt
     6961 tgatttcttt tggttaattt tgtttctaaa ctattgtttt cttttacgat tgctcgtaat
     7021 tgttctggtt ccattatcta atagtcctcc ttagccgttc cttttctcaa cggtcgtcac
     7081 aaaagacatt ctaccatatt ctacagctag tttgatagcc cttttacaca ttaaagcgga
     7141 ataacattac gtcgccgtct tgtacgacat aatcttttcc ttctaatcga acacgtccag
     7201 cttcttttgc tgcatgcatg tttccgtaat gatccaagtc ttcaaaagag acagtttctg
     7261 cacggataaa tccgcgttcg aaatctgaat ggatgattcc tgctgcttgt ggtgctttga
     7321 ttcctttttt gaatgtccaa gcacgtactt cttgttcccc agcagtaaag taagtagcta
     7381 agcctaacaa atcataagca gcacggatca actgatccaa acctgattct tcgattccta
     7441 aagcttctaa aaattccgct ttgtcttctt catctaattc tgcgatttct tcttctgcac
     7501 gagcactgac cacgatcact tctgcatttt cttctgcagc aaagttgcgc acttcttgta
     7561 cataatgatt cgcgtctgca tctgctacgt catcttctga gacattggct acataaagaa
     7621 ttggttttgt cgtcaataaa aatagtgatt taacgatttt ttgttcttct tcagtaaatt
     7681 cgatcgttcg ggcagatttt ccttcttcca gtacaggttt gattttatcc agtacagcta
     7741 gttctgccac tgcatcttta tctttggttt tagcaacttt tgctacacga gtatagcgtt
     7801 tgttgataga ttctaagtct gctaacacta gttccaagtt gatcgtatca atatcttcta
     7861 gtggatcaac acgtccttct acgtgagtaa tattatcatc atcaaaacag cgaacaacgt
     7921 gacaaatagc gtctacttgg cggatatggc ttaagaattg atttcccaaa ccttctcctt
     7981 tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc aaaagtcgtc ggaacagttt
     8041 ttttcggatg tacaagttct gttaagcgtt gcaaacgcca gtcaggtact tctaccatcc
     8101 ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc ttctgctcct gcttttgtga
     8161 tcgcattaaa caaagttgat tttcctacgt tgggcaaccc aacaattcca gcagtcaatg
     8221 ccataaattt attcactctt tctctgcatt tttttcaatt acttttttta atttcttttc
     8281 aaaatctcga cgggtcatca tgacgatatg tccgcaattc atacatctga ttttgatatc
     8341 tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa gcgtgaggtt ttttcatttc
     8401 aacgatatcg tgcaagtcgt acataaaaat agcctccttt ttgtttattc ttcatcaaat
     8461 tgaatattca agatatcaag aattcgagtc aaatcttttt gagaaagata ttcaatctcg
     8521 atcttacctt tcccattttt ttcttggata gctacacttg taccaaattt atccatcaaa
     8581 cgatcttcgc tttctcggat ataatatggt ttttcctttg ctagccttgg gatttttttc
     8641 ttttcttttc catcattttg atttaactca gaaacaagct gttccagttg gcggacagtt
     8701 aaattttcag ttactgcacg attggctaat ttcaagatca atgttttatt ttttaaacct
     8761 aacagcgtac gggcttgtcc catagataaa cgctgatctt gtaccatttc cttgacaaga
     8821 tccggtaaag aaagcaagcg caaataattg gcgatataag gtctgctctt tcctaaacga
     8881 ctagctactt ctgcttgtgt tagtttcagg tttttcatca gcatctcata tgcttctgct
     8941 tcttctaacg gattcagatc ttctcgctgc aagttttcca gcactgctac ttgcatcatc
     9001 gcttcttcgt caaagtctct aacgatagca gggatcgttt ctttttcagc tagtttggac
     9061 gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt ttactgcgga acggcgtacg
     9121 atgatgggtt gaaagacacc tgattgttca atagaattag ctagttcctg caaagatact
     9181 tcttcaaatg ttttacgggg ctgatacgga tttggtcgca gctcattcaa tggtagctgg
     9241 acaacggtat catcttttac atccaccgtc tctaagtttt ctaaatcttg aaacagtgca
     9301 tcgattcctc ggcctaatcc tttacttttc ttcacgactt accacttcct ttgccaatgc
     9361 ttggtagact tcagctcctc gagaacgtgg atcgtagtcg atgatcggtt ttccatgact
     9421 aggtgcttct gataaacgaa tatttcttgg aatgatcgtt tcgtaaactt tttcgcggaa
     9481 gtatttacgt acttcttcta cgacttcatt tcccagattt gttcgtgcat catacattgt
     9541 cagtaagact ccctcgatct ctaattctgg attaaagtgt ttttggacta aacgaaccgt
     9601 attcagtaat tggcttaatc cttccaatgc gtaatactca cactgaacag gtatcaaaat
     9661 cgaatcactt gctgtaaatg aattgattgt cagatggcca agagaaggcg ggcaatcgat
     9721 caaaatataa tcatactgac tgcttacttc tgctaaagat ccttttaatc ttgattctct
     9781 tgccatcatt gacgtcaatt cgatctctgc tcctgctaat tggagagtcg caggcacaat
     9841 actcaaattt tcgtgttcag tgatcaaagt tgcctcatca attggcaatt cattgactag
     9901 aacatcatag atgtctcttg tcacatcagg ttttctgatc ccgacaccac tggtagcatt
     9961 tccttgtgca tccatatcca ctagcaatac ttttttgcca aggctagcaa gacaagcacc
    10021 tagattcacc gtcgtcgttg ttttgccgac gccacctttt tggttcgcaa cagatattat
    10081 ttgtgccatc tcttctcctc ctatttgatt ggctgcttat taggcagtcc gggctttctt
    10141 gggtatttct ttggggttgg tttctttttt tgaataacga tgatatgccg ttgatcagag
    10201 acattcggca aggtaaccgc ctcttctttg aggaatttac cacctaataa agcaatcgct
    10261 tgttttgctt ctgtcaattc ctcttcactt tttgctgctt tcaatgcata aaaatagcct
    10321 tcttgtttag ccaagggcaa acacaattcc gctaacacat tcaaacgagc aaccgcacga
    10381 gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt gcccaaatgt ttctgcccgg
    10441 tcatgatata aggaagcatc gattcctaat aatcgggtta actcagataa gaaggttatt
    10501 cgtttattca gagaatcaac gatcgttatc tgcagttctg gaaaggcaat ttttaaaggt
    10561 atgctaggaa accctgcccc cgaaccaaca tcgcataaag ataaaggttg cgtaaagtct
    10621 acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca gatatacgtc tttttcatcc
    10681 gtaatagctg taaggttcat cttttcgttc cattcgacta atgtccgata ataaatagcg
    10741 aattgattca tttgatgatc ggtaagctct atcccttttt ctgccaagga ttggcgaaat
    10801 tcttctggta tcataaatat tcctttcatt gtgaaacagc aattgtttca cagattctct
    10861 ccctacttta acgcaaaatc tctagacatg caatccttcc atatggattc ttaaaaattt
    10921 gaaaagtaag aaaagccgat agactttttc tttctagtat gactcataaa atatacacag
    10981 aagagagaac tagctttgag caatttctgc aaaaactagt tctctcttct atttattatt
    11041 acgtattacg ctggttcact gactttcgca atcttccctt gttcgatata gaccattagg
    11101 atacttaggt ctgcagggtt tacgccactg attcggctag cttgggcaat tgtttctggt
    11161 tggattttct gcaatttttg ttttgcttct gttgctaatc cgttgattgc tgcgtagtcg
    11221 atgttttctg gaatcttttt cgcttccatg cgttttaatt tttctacttt ttccatggct
    11281 tttttgatgt agccttcata tttgatttgg atttctactt gttcgatttc ttttcgtgtc
    11341 aattcttcat tttctggaat aaaccgtaga agttctgcat aagtaatctc aggacgtctt
    11401 aaaaagtcgc ttgctagtac accatctttt aatggtgccg cattaatcgt tgctaaaaag
    11461 tcttggactt cttctgttgg tttgattcgt acattcatca aacgtttgat ttcttgttca
    11521 actgcttctt ttttcttcaa ataattcttg tattgatctt cttttactaa gcctacacgg
    11581 tgacccattt ctgttaatcg caaatctgca ttatcatgac gtaaaagcag acggtattca
    11641 gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt ttgttaccaa atcatcgatc
    11701 atgacaccga tgtaaccgtc acttcgtttg atcactaaag gttcttttcc ctgaactttc
    11761 aaagctgcat tgataccggc aatcagccct tgtccagccg cttcttcata accgcttgta
    11821 ccatttgttt gtccagctgt ataaagattt tcaacgactt tagtttctaa tgtcggacgg
    11881 agctggtgtg gctctactac gtcatattcg atagcatatc ctgtacgcat catttctact
    11941 ttttctaatc cagcaataga atgaagcatg ttgacttgaa catcctctgg taaagaagta
    12001 gataaacctt gaacatagac ttcttccgta tttaagcctt ctggttccaa aaataattga
    12061 tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga tggatggaca atagcgagcg
    12121 cctacccctt caacgatccc tgtaaacatt ggggcacgat gtaagttgtc tcgaataatt
    12181 tggtgagtcg tctcacctgt ataggtcagc cagcaagatt cctgatctag tttataggca
    12241 ctatctggtg tgctgaagct aaaatgattc ggctctttgt caccaggctg ctcttctgta
    12301 acagaatagt cgatcgtact tgattttact cgaggcggtg ttcctgtttt gaaccgttcg
    12361 atagcaaagc caagttcttt tagatgattc gctagtccaa tagaaggttg agagttattc
    12421 ggacctgatg aatatttcaa ttcaccgata atgatttctc cgcgaagtgc tgttccggct
    12481 gtaataacaa ctgctttggc ccggtagctc gctcctgtgg acgtcactac tccatgacaa
    12541 acaccatctt cgacgatcag ttcttcgacg attccttgtc ttagcgtcaa atttggttct
    12601 ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt tgtctgcttg tgctcgcaat
    12661 gctcggacag ctgggccttt tcctgtgttc aacatacgca tttggatata ggttttatcg
    12721 atattcttac ccatttctcc gcctaacgca tcgatttctc gaacgacaac gcctttagct
    12781 ggtccgccta cagaaggatt acatggcatg aatgctacca tgtccaaatt tattgtcagt
    12841 aataatgttt ttgatcccat acgtgcagca gcaagtgcag cttctgaacc ggcgtgtcct
    12901 gcgccaacaa cgatcacgtc atacgcatct gcttcataat gttccaatcg tttgttcctc
    12961 cttttatttt cctaaacaaa attgactgaa taattgagtg attagctcat cttgtacact
    13021 gtcgccgaca acttctccta gatagtccca gcaacgcgtc atatctattt gaacgagatc
    13081 cacaggcatt cctgaatcaa tacctgcaat gacttcttca agtgagaggg aagctttttc
    13141 taacaacgcg atatggcggg tattagaaac ataagtagcg tctctttctc ctgtttctcc
    13201 gctgaagaat aaatcagaaa tagctgattc caaacgatct agtccgtcgt ttttagctac
    13261 ggaaattgaa aaaactggtt cattttcgat caatttcttc aatttttctt gttccagctg
    13321 tgctggcaaa tctgttttat ttagaagaat gatccgtttc aatccacttg tcgcttccaa
    13381 taattgttca tcttctgctg tcagtggttc gctttggttt agaacaagta agatcaagtc
    13441 tgcttctgct aatgcttttc ggcttctttc gaccccgatc cgttccacca catcttctgt
    13501 ttcccgaatt ccagctgtat cgattagctt gagcggtacc ccccgaacat tgacatactc
    13561 ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg attgcttttt cttctcttaa
    13621 caaatggttt aacaaacttg atttgccgac atttggccgg ccgatgattg ccgtacttaa
    13681 cccttcccgc agaaccttcc cttgcttgga tgtagcaagt agcgcttgga ttctttgttg
    13741 gatcatctgc gctttttcaa gcaataactt cgtagtcaat tcttcgacat catcatattc
    13801 tggataatca atatttactt ctacttgtgc taatgtctct aaaatctctt gtctcaatga
    13861 gcggatcaaa gcagacagat tgccgtctaa ctgattaaga gctagtccca ttgctttgtc
    13921 tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt gaaaggtcaa cacgaccatt
    13981 caagaatgct cgttttgtga attcgcctgg ctcagctaat cttgcacctt ctcttaacaa
    14041 tagctgcaaa atttgattga ctacaacgat cccaccgtga caattgattt ctactacatc
    14101 ttcccgcgta aatgtctttg gtgctctcat cacggagacc atgacttcat cgacaagctg
    14161 attgctcttg ggatcaacga tatgtccata atggatcgta tgacttggta cttctgataa
    14221 tcgtttgttt ccagattgat aaactttatc tgctagttgc acagcttgat cgccacttaa
    14281 acgaacgata ctgattgcac cttctccagg tggggtagaa attgccgcaa tcgtatcaaa
    14341 ttctaaagta atttcagcca ttttttcact tcctttttca ttttgcgcac aaaaaaagtg
    14401 cccactccac cctttatgca caataaacgg tgaacttcgc actttgactg cttgttcttg
    14461 attaaattct ggtcatagat tatcacactt gctataagga atcaagtgtt taaaacaatt
    14521 agatgaagtt gagcctgttc tgcttccatt accttatatt taattgtcct tggatgtctc
    14581 tttgcgttct ttggtttatt cgaagagaag atactgtata aacaaggctt tatctttctt
    14641 aaaaaaaacg gtacactaaa tggtagcgct atctaaacta tacacttttg aaaggagaaa
    14701 atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa agtgaccctc ggatcaacga
    14761 tgtcgtttta tctacaggga atatcaacca gaaatatatc gtccgcgata tcgtttttgt
    14821 tgtggaatgc ttagaagctg atttgtttga cccatctttt cccaaagatg accttttctt
    14881 aacaacgaag aggaaattaa agacgaaggt acttgaatat ggtgcgaatg cagtaatcaa
    14941 ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag tattttctgg aactttttgc
    15001 ttatggaaca gtgatccaat ttatccaaac aacgattggt taaatgaaac agacaaaaaa
    15061 agcggatcaa aactgaagac agtttcgatc cgcttttttc tatcaaagtt agtgactact
    15121 tcatcgtata ctctgataag aatgatttct agtaaaatat acttggtttg aaaaactttg
    15181 tcaacgtgat ttattcaaaa agtaatggaa tcaataatat tttttagcag gttctactac
    15241 taaataacga taaggttcct caccttccga atgtgttttt acatgttcat ctttgcttaa
    15301 tgcagcatgg atttgtttac gttcaaatgc aggcattggt tctaagaaaa caggctgccc
    15361 tgtccgtttt actttttcag ccgtgcgatc agctaaacgc tgaatgatct cttggcgctt
    15421 ttcacggtaa ttgcctacat tgactactac agacagctta tttcttgcta aacgatggat
    15481 gaagacttgt gccagatatt gcaatgcatt cagtgttttt ccatgtttcc caatcagtat
    15541 gccttgcttt tgtgtttcta aatggaaaac aaccatatta tttgattctc tttccatttt
    15601 gacaagagct ggagcatcaa gctgttttga aatttcagta agataaactg ccaattccgt
    15661 taaagctgct tcatcaccta gctcacttac ttcttctgtt ttttcttcaa gaatcgtttc
    15721 gttgactacc tcgatctcag ctgttggttc ttcttccaaa atttcttcta cagtctgttc
    15781 tactgtttca gaaacttttt cgctgatact tggctggatc gaaagccgtg cattctttcg
    15841 acccattcct aggaaacctt tttttccttc ttccagtatt tcgatttcga cttggtcttt
    15901 ggccaaaccc aaatcagcca gacctagttg gacagcttcg tctattgttg cagcttcata
    15961 aatcggcatt agcattacct cccctattat ttccgttttt tcttaggatt cattgctttc
    16021 ttaagagcac gttctctttc gcggattctc cgtgcttctt cttcacgctc ttttcgaatt
    16081 ttgaaagggt tgttgatcag cagcgtttgt acaacttgga aggcattgga aactacccag
    16141 tataatgaaa gaccgctggc taaattgaca cccatcacta aaatcattac aggcatcacg
    16201 aaattcatga tcttcattga tgcattcgat tcaatttgac tcatgctaga taaatacgta
    16261 cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa atgttggatc cgcgttccct
    16321 aagttcaacc ataagaaatg cccttgtttc aactctggta cacgtgaaat agattgccat
    16381 aacgccatca agattggcat ttgtactaat aaaggaagac aaccagcata tggattgacg
    16441 ccattttctg catataaacg ctgctgctct tctcttaact tactttgtgt ttccggatct
    16501 cttgaactat attgttgctg cagtgctttt aatttcggtt gtaattcttg cgttttacgc
    16561 atgctctttg tttggaaatg catcaacggc aataggatca cgcggatgat cagtgtaaat
    16621 aaaatgatcc ctatccctgc attaccaaat gacaacgcct tgatcgcctc agcaaagtaa
    16681 taaacaatat aacgatccca aattcctgta ctctgtgcgc tgacttcccc agttccgcaa
    16741 ccggataaga cgatgaccaa actaacgata ccggccatca aaagtaaacg tttgtatttc
    16801 ttcacttact cttttcctct ctgtcgatta tttttgcaag ttttaagacg tggactaaat
    16861 tggagcgaac ctcatcataa gtcaacccct tcacacttgg tctagcaatc acaataaaat
    16921 ccaactccgg atcaatttct tcttttatag aataaactgc tgctcggatt tttcgtttga
    16981 cttcattgcg gtttaccgcg ttgccgacct tttttcctac agataaacca actcgaaaat
    17041 gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt gcggttagca aaagaatctc
    17101 ccgtatggaa cacggcttga aactctcgtt ctttttttac tcgatacgct tttctcatta
    17161 gttttctttc cttttctatc tctcacttac gctcataatc ataccataat tacgattgtc
    17221 ctttcatcat ttccctaaaa aagagtataa aaaaaaccac tgaaacccca gtggcctaag
    17281 ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa cacacgacgt ccatttttag
    17341 tactcatacg tttacggaat ccgtgaactt tttgacgttt acgtttattt ggttggtatg
    17401 ttcttttcat atattccacc tccaaaatgt tcttatctat tttataaaca cagacatact
    17461 ttgatagtat aacgacaaag tagtgatttt gtcaatataa aatagaagat cgattgttaa
    17521 aacttatcca caagattatt tttttctatg ggaatcctat gtggaaaaag ttatgcacag
    17581 ggattatcca cagataataa aaggctggtt ttacacagaa aacacacgtg taaaaaaagt
    17641 aatccacaat caggcgattt cctgtgtatt ccgtctccta actattggta tatctgtatt
    17701 ttttatccac agacgcaaga ggaaatctat tggtttttac gagttttcca cctgtgcatt
    17761 agtggtgtgg aaaacttttt ttgacaactg tgtttttctc ttttttcttt ttgtcgattg
    17821 tgtggataat tgtaattaaa aaattttttt cttcggtttc tgtggaaaac tattgaaaaa
    17881 aatgctaaat tagtactagt tattcacttt ttaatgaagg aggaggcctt atatggtatc
    17941 cctcgatgct ttatggaatg aattaaaagc aacataccaa aaggatttat cgcctgctag
    18001 ctataataca tggatcgaaa cagcgaaccc tcgaaccctt gatcaaaacc agctcgttgt
    18061 tgaggttcct agcaagattc ataaagaata ttgggaaaaa aacctggcga caaaaatcgt
    18121 tgaagtcggt tatatgttat caggaaatga gattatccct cgctttatta ctggcgaaga
    18181 agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa gttgtggcac ctagtccgct
    18241 aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg tttgtcattg gtaaaggaaa
    18301 ccagatggct catgcagcag cgctcgttgt agcagaagat ccaggttcta tttataaccc
    18361 gctcttcttt tatggaggcg tgggacttgg taagactcac ttgatgcatg caattggtca
    18421 tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat gtaagcagtg aaacttttgc
    18481 taatgatttc atcaattcga tccaaaacaa aacagcagaa gagttccgtc aagaatatcg
    18541 aaatgttgat ttactgctag tggatgacat tcaatttttt gcagaaaaag aagctacgca
    18601 agaagagttt ttccatactt tcaatgcctt gtacaatgaa gggaaacaaa tcgtactaac
    18661 cagtgatcgg ttgccaaacg agattccgaa actgcaagaa cgccttgttt ctcgctttgc
    18721 atgggggctg tctgttgata ttacaccgcc ggatcttgaa acaagaacag cgattttacg
    18781 taaaaaagcg gcggctgaac gtctagagat cccagatgac acactaagct acatcgcagg
    18841 acaaattgat tctaacattc gtgaattaga aggtgcgctt gttcgagtgc aagcttttgc
    18901 tgcgatgaat agtgaagata tttcgactag tctagctgca gacgcactga aaacgttaaa
    18961 atcaggaaaa ggacatcctc aattgtcaat cctgcagatc caagaagaag tggcaaagta
    19021 ttatcacatc caattgaaag atctcaaagg aaagaaacgc gtcaaatcta ttgttgttcc
    19081 tagacaaatc gctatgtatt tggcacgtga gctcacagat aattctttac cgaaaatcgg
    19141 agctgaattc ggtggcaagg atcatacaac ggttatccat gctcatgaaa aaatccagca
    19201 gctgatggat tctagtgtca gtatgcaaaa tgaagtatca gaaatcaaaa actttttact
    19261 aaattaattt gtcgattcta tcaactgaaa atagcatgtg gataaaagaa aaaaacgttc
    19321 aaaagttata cacacttttt ccacagctgt tttttcgttt ctctcttacg ttttttgagt
    19381 tttccacaga atcaacatgc cctattactt ttattattct ttttaactaa taataaataa
    19441 acaaacattc aaaggagata catcatgaaa gttactttaa accgagctag ctttatgcag
    19501 gaattgcaaa ctgttcaacg agctatttca agcaaaacca cgatccctat tttgacaggt
    19561 gtaaaaatca cactgacaca agaaggtttg actttgacgg ggagcaacgc tgatatatca
    19621 attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata tgcaaatcga atctactggt
    19681 tccattgttt tacaagcacg tttctttagc gaaatcattc ggagacttcc tgaagaaaca
    19741 tttactttag aagttttaga aaataaacaa gtagcgatca cttctggaaa agcgaatttt
    19801 atcgtaaatg gattagatgc agataactat cctcatcttc ctgttgtcga aagccataac
    19861 cagatgaaat tacctgtaca cgtattgact aaactaatca acgaaacagt ttttgctgtc
    19921 tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt tcattttatc tgataattct
    19981 ttattagctg tagctactga ttctcaccgt ctaagtcaac gcgtgattcc agtagaacaa
    20041 gcggctgatc attttgatat tgttattcct ggaaaaagtt tgatcgaatt atctcgctca
    20101 ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg aaaaccaagt gttgttcaaa
    20161 acagaaacga tgtatttcta ttcccgtttg ctagaaggaa actatccaga taccaatcgt
    20221 ttgattcctt caagttttaa tacagaggtt gaattttctg ttcctagctt tttagcggcg
    20281 atcgaacggg cttctttact ttctcatgaa ggccgtaaca acatcgttcg tttatctatt
    20341 cgtccagatg cggttgtttt atacggaaat tcgccagaaa tcgggaaagt agaagaaagc
    20401 ttgagttata cagcaagcag tggcgatcca ttagatattt ctttcaatcc ggattatatg
    20461 aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag tgaaatttat ctctgctatt
    20521 cgtccattta cattggaacc aacggaagat ggcgttcaat ttattcagct gattactcct
    20581 gtacgtacaa attaacaatg attttcaatg ttcttgaaaa tttaattgaa aagaaataga
    20641 aaaaataaaa ttttaaacag aaggccctaa aatctctttt aaggctttct gtttattttt
    20701 gtttaaatca ccttttttag attaaatcgt cttatatgga aaaatatatg gataaatttg
    20761 ttttattgcg aaaaaaaagg tataatagag tatactttta ataatgaaaa tgagggatgg
    20821 gcattgaaac aacaagtcat tctaaatacg gaattcatga cgttgggcca aatgttgaaa
    20881 gaagtcacag ttatcggtag tggcggacaa gcaaaatggt atttggcaga aaacactgtg
    20941 ttagtcgatg gcgaaccaga aaatcgtcgt ggacgcaaac tttatcccgg catgatggtt
    21001 gaagttccag aagtaggaac tttttttatg gtaaaaaaag gaaacgacga taatgaggct
    21061 gaataagctg tatttaaaaa attatcgaaa ttacgaagag ctgaacatcg aattttccaa
    21121 gaacctcgtc atctttttag gagaaaatgc gcaaggaaaa acgaatcttt tggaaagtat
    21181 ctacgtatta gctatgacgc gaagtcatcg gacaagtagt gagcaggaat tgattggctg
    21241 gactgacgat caggcgatga ttcaaggaga gatcacaaaa ggttcatcca ctcttccatt
    21301 agaaatctta ttatctaaaa aaggccgaaa aaccaagatc aaccatatcg aacagaaaaa
    21361 gttaagtact tacgtgggac aaatgaatgt cattttattt gcaccagaag atctttcttt
    21421 agtaaaaggc agcccgcaga ccaggcggaa gtttttagat atggaattag gccagatcaa
    21481 tccaatctat ctttatgatc ttgtccaata ccaagcaatt ttgaaacaaa gaaaccagta
    21541 cttgaagcaa ttgaatgaaa aaaaacaaac tgatttgctt tatttggata ttttgtccga
    21601 acaactagca gcgtttggca gcaaagtatt gaaagcaaga gcacagttcg tcaagcgatt
    21661 ggagttttgg gctaattctt tgcatcagca aatcacccat caaaaagagc agctggaaat
    21721 cgagtatctc accgcagtcg attctttaga aacacatacg caagagcaaa tccaagaaca
    21781 atttctcgca cttttgaacc aaaataaaaa gaaagacctt tttcggggaa caacgacagt
    21841 tggtcctcat cgtgacgatc tttccttttt tattaatcag aaaaatgtgc agacttatgg
    21901 atcgcaagga cagcaaagaa caactgcact aagtgtcaaa ttagcagaaa tcgatttgat
    21961 taaagaagag acaggggaat atccaatcct tttgttagat gatgtgatga gcgagctgga
    22021 tgataatcgt caactccatc tgctagaaac aatcgaagga aaagttcaaa cgttcttaac
    22081 aacaacgaca cttgaccatg taaaaaataa aatgactgtt gaacctgaga ttttttatgt
    22141 tcaacaaggg aaactaaaag gagaagaaac tgaatgacag aagaaagaag tttagtagaa
    22201 cgcgctaaag agtatgatgc cagtcagatt caagtacttg aaggacttga agccgttcgt
    22261 aaacgacctg gcatgtatat cggttcgact agttctgagg gtctgcatca ccttgtttgg
    22321 gaaattgtcg ataattcgat cgatgaagta ttagcaggtt ttgcaacgaa aatccatgtg
    22381 attattgaaa aagacaacag tatcaccgtt attgacgacg gtcgggggat acctgtagat
    22441 atccaggcaa aaactggacg tcctgcagtt gaaacggtct ttactgtttt acatgctgga
    22501 ggaaaattcg gcggtggtgg gtacaaagtt tctggtggac ttcacggagt aggatcttct
    22561 gttgttaacg cgctttctac acagcttgat gtcaaagtat acaaagaagg aaatgtgtac
    22621 tatcaagaat atcgtcgtgg agctgtagtg gacgacttaa agattatcga acaaacggat
    22681 cgccatggaa caacggttca ctttacgcca gaccctgaaa tttttacaga gacaactgag
    22741 tttgattttt caaaattggc tacccggatc cgtgaacttg ctttcttgaa ccgcgggatg
    22801 cgtatatcga ttgaagacaa acgagaagaa gaaccagtca tcaatgaata ccattatgac
    22861 ggcgggatta aaagttatgt cgaatacttg aatgcaaaca aaaccgtgat ttttccagaa
    22921 cctgtatatt tggaaggtga acagcaagat atcgcagttg aagtctctat gcaatataca
    22981 gacggctacc attccaatat catgagtttt gccaacaata tccatacgta tgaaggcgga
    23041 acacatgaat caggattcaa gactgcttta actcgtgtga taaatgatta tgcccgaaaa
    23101 caaaagctga tgaaagaaaa tgacgacaac ttgaccggtg aagacgtgcg agaaggattg
    23161 acagctgtta tttctatcaa gcatcctgac cctcaattcg aaggacaaac aaaaacaaaa
    23221 ttgggcaatt ccgaagtacg gacagtcact gatcggttat ttagcgaaca tttcatgaaa
    23281 ttcttgctgg aaaacccaag tgtgggccgt caaattgttg aaaaaggatt actagcttcc
    23341 agagctcgtc tagcagcgaa acgtgcgcgt gaagtaacga gacgtaaagg tgctttagag
    23401 attagtaatc tgccaggaaa attagcggac tgctctagca acgatcctga aaaatgcgaa
    23461 ttgttcatcg tcgaaggaga ttcggccggc ggatcagcta agcaaggtcg taatcgagag
    23521 tttcaagcca tcttgcctat tcgtgggaaa atcttgaatg ttgaaaaagc aagcatggac
    23581 aagatcttgg caaatgaaga gatccggtct ttatttactg ctatgggtac agggtttgga
    23641 gaagattttg atgtatcgaa agctcgctac cacaaattag ttatcatgac tgatgccgat
    23701 gtcgacggcg cgcatatccg tacattgctg ctgactcttt tttatcgtta catgcgtcct
    23761 attgttgaag ctggatacgt ctatattgct cagccacctt tatacggggt aaaacaaggg
    23821 aagaatatta cgtatgtaca accaggaaaa aatgcagaag aagagttgaa acaagtcgtt
    23881 gcttcacttc ctgcctcacc aaaaccaagc gttcagcgtt ataaaggact tggagaaatg
    23941 gatgatcatc aactatggga aacaactatg gacccaagca atcggatgat ggcccgcgtc
    24001 agtgtggacg atgctattgc agcagatcag atatttgaaa tgttgatggg ggatcgagta
    24061 gaaccccgtc gtgcattcat cgaggaaaat gcacactatg tgaagaactt agatatttaa
    24121 tcatgaagga ggaaaaagaa tgagtgaaga aatcagagaa aacatccatg atgtcaatct
    24181 aaccagcgaa atgaaagact ccttcatcga ttatgcgatg agtgttattg tcgcacgtgc
    24241 acttcctgat gtcagagatg gtctgaaacc cgttcatcgc cgtattttat acggcatgaa
    24301 cgaattaggt gtaacaccgg ataaagccca taaaaaatct gccagaatcg tcggagatgt
    24361 catgggtaaa taccatcctc atggagacta tgcgatctat gagtcaatgg tacggatggc
    24421 acagccattc agctaccgtt atatgctagt ggatggtcat ggaaactttg gttctgttga
    24481 cggtgacggt gccgccgcta tgcgttatac cgaagcacgg atgagcaaaa tcgctacaga
    24541 aatgctgcgt gatatcaata agaacactgt tgatttccaa agcaactatg atgatacaga
    24601 aaaagaacca gttgtactac ctgctcgctt cccgaatctt ttagtgaacg gaacaacggg
    24661 gatcgccgta ggtatggcca cgaatatccc acctcataac ttgtctgagg tagtagcggc
    24721 tatcgatttg ctgatggata atcctgatgt gacgaccaat gaattgatgg aagtattacc
    24781 tggaccagat tttccaacag gtggtttggt tatgggaaaa tcaggaatcc gaagagcgta
    24841 tgaaacaggt aagggttcta ttaccgtacg tgcaaaagtt gagattactg agatgccaaa
    24901 tggtaaagaa cggatcatcg tgacagaatt gccttatatg gtcaataaag caaaattgat
    24961 cgaacggatt tctgaactgc acagagacaa gcgtatcgaa gggatcacag atttgcggga
    25021 cgaatcttcc cgtgaaggta tgcggatcgt cattgacatc cgtcgtgacg ccagcgcatc
    25081 tgttatcttg aataacttat ataaattgac atccttgcaa aattcatttg gattcaatat
    25141 gttggcgatc gaaaaaggtg tgccgaaagt tttgagcttg aaacaaattc ttgaaaacta
    25201 tatcgaacat cagcgcgaag tcatcacaag acggacagtc tttgaaaaag aaaaagcaga
    25261 agcaagggca cacattttag aaggtttgcg tattgctttg gatcatatcg atgaaatcat
    25321 tgcgattatc cgcgggtcta aatcagatga cgaagcaaaa gcgacgatga tcgaacggtt
    25381 cgatttatcc gatcgccagt ctcaagctat tttagatatg cgtttacgcc gattgaccgg
    25441 tttggaacgg gaaaaaatcg aaaatgaata ccaagaactc ttaaaactga ttgaagattt
    25501 aactgacatt ctggcaagac cagaacgtgt tacagaaatc atcaagaatg aattagcaga
    25561 cttgaatcaa cgatttggtg ataaacgacg cacagaatta ctagtcggtg aggtcttaag
    25621 tctggaagat gaagatttaa tcgaagaaga agaaatcgtt attacattga caaacaatgg
    25681 ttacatcaaa cgtttggcaa ataatgaatt cagagctcaa cgaaggggtg gacgtggtgt
    25741 ccaaggaatg ggtatccaca atgacgactt cgtcaaaaat ctggtttctt gttctaccca
    25801 tgatacattg ttattcttta cgaataacgg aaaagtgtat cgggcaaaag gatacgaaat
    25861 tccagaatac gggcgcacag ccaaaggaat accaattatc aatttactag gtatcgattc
    25921 gagtgaaagc atccaggcaa tcattgcggt gacgggagaa gcagaagaag gccactatct
    25981 cttctttacg acgcgtcaag gtacagtaaa acgtacttct gtcactgctt ttgctaacat
    26041 ccgaagtaat ggtctgatcg caattggttt gaaagaagac gatgagctcg tcaatgtgct
    26101 actgacagat ggtcaatcta atatcattat tggaacacat aacggctact ctgtcacttt
    26161 caaagaagaa gcagtccgcg atatgggacg tacagcttcc ggggttagag gaatacgcct
    26221 gcgtgaggaa gattatgtcg ttggggcagc cttaatgaag gaagatcaag aagtattagt
    26281 gatcacagaa aaaggttacg gaaaacggac aaaagtatcg gaatatccag tgaaaggccg
    26341 cggaggtaaa gggatcaaaa cggcgaacat cactgaaaaa aatggtccac tagctggatt
    26401 gaccactgtt tctggagatg aagacatcat gctgattaca gataaaggtg tcattatccg
    26461 tttcaacgta tcgactgttt ctcaaactgg acgttcaaca ttaggcgtac gtttgatgaa
    26521 gatggaagct gatacgaaag ttgtcacgat ggcagcagtc gaaccagaag ctgcagaaca
    26581 aacagttgaa gaacaaggat cagaagaata gccatcaaga ctaattgtgt aagctagcaa
    26641 gaatgaccga ttaaaaaatc tggttattcc tgctggcttt tttgattagt tgaatttctc
    26701 ttaaaatatt cgaaactgtt cattctgtat tgcttttgac caaaatagag tgtataatca
    26761 gagcttgtga gtagtctaat taattagata actctccttg ctcttaggaa cctaagagcc
    26821 gaaagtccac agggaggtga atatcaatgg aaaacacgaa atatgaaatc atgtacatta
    26881 ttcgtccaaa cattgatgaa gaagcaaaaa ctgctttaat cgaacgtttc gatgcaatct
    26941 tgaaagataa cggagcagaa gttatcgaat caaaagattg ggaaaaacgc cgtttcgctt
    27001 atgaaatgaa cggttaccgt gaaggtatct atcacatcgt taaagtatct tctccatcat
    27061 cagcaaatgc tgtcaatgaa tttgaccgtc ttgctaaaat caacgacgat atcattcgtc
    27121 acatgattgt taaagaagaa gcttaattgt tgtttcatgt gaaacaatag aaaacgatga
    27181 gaggagatga ctattttgat taataatgtt gtattagttg gacgtctgac gaaagatcca
    27241 gatttgcgtt acacagcaag tggtacggca gtcgcaacat ttactttggc tgtgaatcgt
    27301 aattttacaa accaaagcgg caatcgcgag gctgatttta tcaactgtgt gatttggcgc
    27361 aaatcagcag aaactttagc aaactatgct cgtaaaggaa cacttttagg tgttactgga
    27421 cgtatccaaa ctcgttctta tgataatcag caaggacaac gtgtatacgt gacagaagta
    27481 gtagctgaca acttccaatt gctggaaagt cgttctgcat ctgaaaatcg ccaacaaagc
    27541 ggcggattcc aaagttctgg tcaatcagct ggcggatttg gcggaaacaa taactctaat
    27601 caaacatctc aatcatccaa cggtatgcct gatttcgacc gtgatacttc tgacccattc
    27661 ggctcaagtt caacgatcga catttcagac gatgatttac cattctaaac gaatttgata
    27721 ggagggaaat agaatggcac aacaaagaag aggcggacgt aaacgtcgta aagtagacta
    27781 cattgctgct aaccatattg aatatatcga ttataaagat atcgaattac taaaaagatt
    27841 tatttcagaa cgcggtaaaa tcttgccacg tcgtgtaact ggtacaggcg ctaaaaacca
    27901 acgtaaaatt actgttgcaa tcaaacgcgc tcgtatcatg ggcttgctac ctttcgttag
    27961 cgaagactaa taaatcctag gaattgaatg actgatcctt ttgatcagct attcaattcc
    28021 tttttttctg taaaaataac ctctattcgt ttagtcgctc gaatgacgaa agttatggta
    28081 aaatcaaatt agtcatatat gggaggaaaa ttatggaaaa taaaaacaat catttatccc
    28141 cgaaacctct cttccttatc ctgtttatat taatcgaagt attggtagtc ttaattttac
    28201 catttcgttt tgttagcgct tcggtcattc tattgatgaa tttatggggt gttcgctatt
    28261 ggttgaaaat gaaacgttgg cttattttaa ataaacgtga aaaaatccag caggcttcga
    28321 ctgtagctga aaccaatcta gcttatgtct cagaagtcat gcctgtagga gtgattgctt
    28381 atcagccatc aagccaaaaa atcgaatggc tcaatccatt agcattgaaa atcaaagatc
    28441 aaagtggatt aacagaggaa gaattgctag atactttttt tactgcaaaa gaaaaaaaga
    28501 aaaaccaagt ctatctagca gataaaacat ttcagtttga tatggataca gtaaagggtg
    28561 ttgtttattt taatgatatt acccaaaacc agttgttaaa actccatcag acgaacagtc
    28621 agccggtgat tggtatttta tctattgata actatgcaga tgccattgat aaattggatg
    28681 acaaagagat ttcttatttg aactctttcg tcactacact agtttctgac tggatgaatg
    28741 aataccatgt ctttttcaaa agaatcaacg cagaacggtt tttcttcgta gcacagatga
    28801 tcgatctgaa aaagatgatg gataaaaaat ttgatttgct ggatcgcttt cgagcagaag
    28861 ctgaaaaaca gagctcgcct atcaccctta gtatggggat tgcttatggc tcaagaaccc
    28921 tcgatgaaat cggtggagaa gctcagaata atttagatgt tgctttagtc cgtggaggcg
    28981 accaagttgt cgtgaaagag gtaaaagaag gagcaaaacc tgtttattat ggggggaaat
    29041 ctgctaagaa tgtcaaacgc acgcgtgttc gttctcgagc gatgagtacc gctttgcgtc
    29101 ggatttttga tgaaacaggc gatgtgttta tcatggggca caagtttccg gatatggatg
    29161 ctttaggctc tgcttttggt gtcagttgtc tggcacgctt caaccagaaa aaagcttaca
    29221 tcgttatcaa cgagaacgag atcattccag atgtagaacg gtgtcttgaa gaaatccata
    29281 agcatcctga attagaaatg caactaatct ctccagaacg tgccatagag ctgaaaaaag
    29341 aagaggacct cctcgtgatg gtggattacc ataaaccttc tctatcgatc tctcagggcc
    29401 tatatgagca atttgataag attgtgatca ttgatcacca tcgtcgtggg gaagagtttc
    29461 caagtaaacc tctgctgact tatatcgaat cttcagcttc ttcagcttca gagctagttt
    29521 ctgagctgat ccaataccaa agcagcaaaa caaatcgctt agatcgtttt gaggccacat
    29581 tattgcttgc agggatggtt gtagatacaa atagtttcaa tgtccgtaca actgcacgaa
    29641 cttttgacgt tgccagctat ttaaggtctt gtggagcaga cgcatcctta gtacaatatt
    29701 tattgagctc ggatttagcg acatacttgg agatcagcaa tttgattgct ggaagcgact
    29761 atgtgacttc tgatattgtt gtggcggcag ggacagacga aaaagagtat aatagtgtta
    29821 tagcagcaaa aacagcagat acattgcttt cgatgatcaa tatcaatgca gctttcgtca
    29881 ttacaaagcg gacagacggc ttgattggga tcagtgccag aagtaccggc acgatcaacg
    29941 tccaaatcat catggaatcc ttaggtggcg gaggacactt cacaaacgca gctactcaac
    30001 tggaaaatac gacagttgag gaaacaaaac agaagctatt tgctgcaatc aaagaaaata
    30061 tgaatcaaat atatgataag gagtaatcca aatgaaagta atttttttac aagatgtaaa
    30121 aggaaaaggt aaaaaagggg acatcaaaga agttccagca ggttatgcac aaaattatct
    30181 aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt atcagtcagt tagcaggaca
    30241 aaagaaagca caagaaaaac acgaagctga aatcttggaa gaagctaaaa agttaaaaga
    30301 attcttggaa aaagaagata ctgtagtcga attgaaggcg aaagcagggg aagacagccg
    30361 tttgtttggt tcgatccctt caaaacaaat tgctgaaggg ttaaacaaac aatacaaaat
    30421 caaattagat aaacgaaaaa tcgaattaga aaatccgatc cgttcactag gctacacaaa
    30481 cgtacctgtt aagcttcatc atgaggtgac ggctacgatc aaagttcatg tcgtacaaga
    30541 ataacaaatc ttgatagcga tgttcagctg gtaagtataa ccagaaggaa aaaagacaaa
    30601 ccgaaattta tctgagaaaa cagctgcagc aatgaaagca aaagctgcaa atcgatttca
    30661 gattgatttc ggtttctttt ttgggaacag caaaatagca taaagaaaac gagtataata
    30721 aaagagaact atttttttat tacactttcg gtacgaatga tgacttttct ctcattttct
    30781 tgtaaaatga agcaattgaa gacaaggagc gatacgtatg aatgaagtgt ggcaggatcg
    30841 aatcccaccg caaaatattg aagctgaaca agcggtttta ggctctgttt ttcttgacgc
    30901 agaaacgatc attgatgcca tggagtacat agagccaaaa gacttttatc gtcgtggcca
    30961 tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt aatgaagcga ttgacgtggt
    31021 tactgtcaaa gaccggctgg aacaagccaa tttgttagaa gatgccggtg gtttaagtta
    31081 tctctcagac ttggcattag cagttccgac agcagcaaat atcgtctact atgcgaaaat
    31141 cgttgaagaa aaatcattgc tgcgtacatt gatccagaca gcaacaggaa tcgttacgaa
    31201 aggattcgag caaggagaag aagttcaaac aattttagat gatgccgaac gcagtatctt
    31261 ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct attgctgaag tactgaactc
    31321 cacgattgaa aatatcgatc gactagctca aaacaatgaa gaaatcacag gtctaccaac
    31381 tggctatcaa ggtctggata aaatgacagc aggtttccag cccgaagaac taatcattct
    31441 tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg aatattgctc aaaatgtagg
    31501 aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa atgggggcag aatctcttgt
    31561 aaatcggatg ctttgtgcag aaggttcgat tgaggccagc catttacgta caggtcagct
    31621 ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg agtctttctc gagcaaatat
    31681 ttttatcgat gatacaccag ggatcaaaat ctctgaaatc cgtgcgaaat gtcggaaact
    31741 agcgcaagaa aaaggcaatc taggcttgat attgatcgat tatttgcagt tgattgaagg
    31801 taacggtaaa gagaaccgtc aacaagaagt atcagatatc tctcgccaac tgaaaaaact
    31861 ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag ttatctcgtg gagtagaaca
    31921 gcgacaagac aaacgaccag tgctaagtga tattcgtgaa tccggatcaa ttgaacaaga
    31981 tgcggacatc gtagccttct tgtatcgaga tgattattat gaaagagaag gcggagatga
    32041 cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa gtcatcatcg agaagaaccg
    32101 aagcggtgca cgaggaacag ttgaactact gtttatcaaa gaatacaata agttttcgtc
    32161 gctttcgcca agagaagaat tttaaagaag aggttgtgag ccttgagcaa tcccgagagc
    32221 tctcgtaaga agatgcaagg agtaaccgca ggaacgacta gagtacttaa tttaaacaga
    32281 tctccaaagg gattgtgaca aaagtcttgt cacagtccct ttttccgaat aaacggtgtt
    32341 caatcagctg acccttggta ttagctcagc aaacgtcaaa atatgaggaa ctattttgac
    32401 gtttgtttct ctaatactca ggtcaaaacg ctgatcttac gacctctttt ccttaaacgg
    32461 tgttctcctg ccaactcctc ggtcacaagt cacaatatca tcaaacatga gaagcagttt
    32521 tttgaaattg ctccttgtga ctcagagtta aacggcaggc tcacaacctc tttcttaaac
    32581 ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca aacgaaaaat atgagaagct
    32641 atttttgttt ggctgttctt attacttgat gcaggacagc tttttcacaa cctctgattc
    32701 acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa atttcacaaa aatttgagaa
    32761 gcaattttcg tacattcctt cttatttctt gaagctgacc acttctgtta caacctcttt
    32821 tttaataacc attctaaaaa gataaagttc gttttttaag cgtttttata aaataaaaat
    32881 aaaaatcatt cg
//
LOCUS       DARDHA010000013        32886 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000013 DARDHA010000000
VERSION     DARDHA010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.013"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            59..2335
                     /locus_tag="SDA10_000532"
     CDS             59..2335
                     /locus_tag="SDA10_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4239462.1"
                     /translation="MDGVSVAFRQKEFVAILGPSGSGKTTMLNVIGGLDNYDSGDMVI
                     NGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIENVELGMTLSGVSKDEKRKRA
                     EDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDPDILLCDEPTGALDTETSIQI
                     MELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKILTDSHPHIERPKDDQFNLRR
                     TKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIAIVLSLSSGFQKQIDNTQAET
                     MAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAKVSEEDKAQHTNKIDQTYVDY
                     VTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQNPDTESLSLSSTMSAMTGVG
                     VSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIVDGNNNTNINALKNLGFDVKE
                     DEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYDAMYQNASDELKISGILRVKS
                     SSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSGVNVLTTEKVDENAKQTLLSY
                     LGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDEDKIIYTDLAGTMTELTGGLMD
                     AITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGVLKALGARKKDITRVFDAETC
                     ILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLNPVHAIILVIVSTILTMLGGH
                     LPARMAAKKDAAIALRAE"
     gene            complement(2450..2761)
                     /locus_tag="SDA10_000533"
     CDS             complement(2450..2761)
                     /locus_tag="SDA10_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239463.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            2942..4417
                     /locus_tag="SDA10_000534"
     CDS             2942..4417
                     /locus_tag="SDA10_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEG4239464.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            4464..5048
                     /locus_tag="SDA10_000535"
     CDS             4464..5048
                     /locus_tag="SDA10_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEG4239465.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            5050..5469
                     /locus_tag="SDA10_000536"
     CDS             5050..5469
                     /locus_tag="SDA10_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HEG4239466.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(5750..6034)
                     /locus_tag="SDA10_000537"
     CDS             complement(5750..6034)
                     /locus_tag="SDA10_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEG4239467.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(6386..7096)
                     /locus_tag="SDA10_000538"
     CDS             complement(6386..7096)
                     /locus_tag="SDA10_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEG4239468.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(7093..8565)
                     /locus_tag="SDA10_000539"
     CDS             complement(7093..8565)
                     /locus_tag="SDA10_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG4239469.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            8703..9176
                     /locus_tag="SDA10_000540"
     CDS             8703..9176
                     /locus_tag="SDA10_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG4239470.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(9329..9880)
                     /locus_tag="SDA10_000541"
     CDS             complement(9329..9880)
                     /locus_tag="SDA10_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG4239471.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            9985..10515
                     /locus_tag="SDA10_000542"
     CDS             9985..10515
                     /locus_tag="SDA10_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HEG4239472.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(10998..11441)
                     /locus_tag="SDA10_000543"
     CDS             complement(10998..11441)
                     /locus_tag="SDA10_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4239473.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(11457..12617)
                     /locus_tag="SDA10_000544"
     CDS             complement(11457..12617)
                     /locus_tag="SDA10_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG4239474.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            13124..14824
                     /locus_tag="SDA10_000545"
     CDS             13124..14824
                     /locus_tag="SDA10_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239475.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(14926..15759)
                     /locus_tag="SDA10_000546"
     CDS             complement(14926..15759)
                     /locus_tag="SDA10_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEG4239476.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(16063..18228)
                     /locus_tag="SDA10_000547"
     CDS             complement(16063..18228)
                     /locus_tag="SDA10_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4239477.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            18488..20782
                     /locus_tag="SDA10_000548"
     CDS             18488..20782
                     /locus_tag="SDA10_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEG4239478.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            20775..21440
                     /gene="pgmB"
                     /locus_tag="SDA10_000549"
     CDS             20775..21440
                     /gene="pgmB"
                     /locus_tag="SDA10_000549"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEG4239479.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            21433..22440
                     /locus_tag="SDA10_000550"
     CDS             21433..22440
                     /locus_tag="SDA10_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG4239480.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            22462..23481
                     /locus_tag="SDA10_000551"
     CDS             22462..23481
                     /locus_tag="SDA10_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4239481.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(23762..25129)
                     /gene="nhaC"
                     /locus_tag="SDA10_000552"
     CDS             complement(23762..25129)
                     /gene="nhaC"
                     /locus_tag="SDA10_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEG4239482.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(25239..26660)
                     /locus_tag="SDA10_000553"
     CDS             complement(25239..26660)
                     /locus_tag="SDA10_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4239483.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(26903..28780)
                     /gene="tdc"
                     /locus_tag="SDA10_000554"
     CDS             complement(26903..28780)
                     /gene="tdc"
                     /locus_tag="SDA10_000554"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEG4239484.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(29071..30327)
                     /locus_tag="SDA10_000555"
     CDS             complement(29071..30327)
                     /locus_tag="SDA10_000555"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEG4239485.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(30439..30644)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            30907..32208
                     /locus_tag="SDA10_000556"
     CDS             30907..32208
                     /locus_tag="SDA10_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEG4239486.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(32277..32876)
                     /locus_tag="SDA10_000557"
     CDS             complement(32277..32876)
                     /locus_tag="SDA10_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4239487.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT        10099 a         6730 c         5972 g        10085 t
ORIGIN      
        1 gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca ccaaagcatt
       61 ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag gtcctagtgg
      121 ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg attccggtga
      181 tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg atgcctaccg
      241 aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc taggcattat
      301 tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa aacgaaaacg
      361 ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa agccaaatca
      421 gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca atgacccaga
      481 tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta tccagatcat
      541 ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac ataatcctga
      601 actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga tcttaacaga
      661 ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca gaacaaagat
      721 gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa aaggacgtac
      781 tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg ttctgtccct
      841 ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg cgaaatttcc
      901 gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat tgggtgcaag
      961 caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag aagataaagc
     1021 acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca tcgatcctaa
     1081 cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac gtgatgtaaa
     1141 tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat ctctttcttt
     1201 atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta cacagcttga
     1261 cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa gttatccagc
     1321 ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata tcaacgcatt
     1381 gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg atatcgttgg
     1441 tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa ctgggaattt
     1501 tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat tgaagatctc
     1561 ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag gaatcgctta
     1621 tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa tcgtcaaagc
     1681 tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa atgccaaaca
     1741 aacattgctt agttatttag gtggagacag cttgccttca agtatcatga tttatccgaa
     1801 caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata aaggcaaatc
     1861 agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac tgactggcgg
     1921 attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt tagtaacaag
     1981 tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa aagaaatcgg
     2041 tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg acgcagaaac
     2101 gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc tagctacgtt
     2161 ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc aactaaatcc
     2221 tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg gcggtcatct
     2281 tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag aataaggttg
     2341 tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa cagcaagaaa
     2401 cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt tataattctg
     2461 gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct tttccattta
     2521 tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata ttacgagaaa
     2581 gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag gcatcggtta
     2641 tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat gtaactttca
     2701 aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc atttttttca
     2761 ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata tcatagttta
     2821 ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa caagttgaat
     2881 tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg aggaatctct
     2941 catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa cacttccagg
     3001 tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg gtgtttaccg
     3061 ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag ctatgacttt
     3121 caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg ctagtcgtaa
     3181 acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct tgaaagacgc
     3241 tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag cacaagaagt
     3301 cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg cacctacaaa
     3361 tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat cagatccaga
     3421 aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag atacgatcgg
     3481 tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat tcgaagcctt
     3541 gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg ccattggcac
     3601 gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg caggagcttt
     3661 gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg gtattgctcg
     3721 tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag aagatatcaa
     3781 cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg ctaatccagc
     3841 tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa tcatgcaaac
     3901 aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag aagcaagtat
     3961 tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca atcctcctgc
     4021 aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag cggatgaagg
     4081 tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga agacagtcat
     4141 tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga ctttcaacac
     4201 tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc atccattgga
     4261 cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga tgagtgcaaa
     4321 aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac gtgcaaaatt
     4381 ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca actgttttga
     4441 catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa aaaatcgtga
     4501 ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag aaactaaaag
     4561 aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag aacttaaagc
     4621 tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat tattcaaaca
     4681 aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg atcatcgtac
     4741 cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac ctgattgcac
     4801 gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct cgtgaaacgc
     4861 cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt gtgcaaatca
     4921 taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt gttaatcacc
     4981 atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa cgttggcagg
     5041 gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt cacgatccaa
     5101 gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg agatacacca
     5161 catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt acgtttccct
     5221 agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc tgtgatcatc
     5281 aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga ccatatctcc
     5341 aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa gattttagat
     5401 tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga tggtgaaaaa
     5461 acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag agaaaaaaat
     5521 ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac agctgattga
     5581 acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa gaaaaatttt
     5641 gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa actggctttt
     5701 gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt tatttttgct
     5761 tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga atatcttttc
     5821 gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata ttcacgatac
     5881 tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca gcaccgaatt
     5941 cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact tcggaagcat
     6001 agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt tttacatcta
     6061 gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag ttcctttttt
     6121 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt caacaaacat
     6181 gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacaacc
     6241 tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat cagatgaaaa
     6301 atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca gctttttcac
     6361 aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact aattttgcga
     6421 atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc tctgctttgc
     6481 tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa gcttttgctt
     6541 cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct gcttggtccg
     6601 tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg tcatattcgt
     6661 gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat tcccaataag
     6721 tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt gtttcgttat
     6781 ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga tacacttttt
     6841 cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa tcagcactgg
     6901 aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga ttggacaaat
     6961 tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa gataaaaagg
     7021 tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag ctgacaatca
     7081 aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt ttttcttcaa
     7141 taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa acgcggcatg
     7201 gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg tattatccaa
     7261 tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga tcaaaatagc
     7321 tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc catgactgat
     7381 caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga ccatggagat
     7441 cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg tttttgtcaa
     7501 gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg ctaaacgttt
     7561 cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca tgatcaatgc
     7621 acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat tttgaatata
     7681 caatggtacg accatttctg cccctaccat cgccatattt gttacaccag ctaaaacagc
     7741 tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat gttccaaatg
     7801 taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga aaccaattac
     7861 tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta aactaccgaa
     7921 tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca atggaataac
     7981 acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca gtaccatacc
     8041 aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag ggcctaaagc
     8101 aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt tttctggcgg
     8161 gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc cgcaagcttg
     8221 gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag taatcgttcc
     8281 gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt tattgatcag
     8341 ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg ttaaccattg
     8401 cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg tcgtcaataa
     8461 ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta aaagtgtccg
     8521 attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc tccttctttc
     8581 tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt gtcaacctac
     8641 ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa ggagtgacaa
     8701 aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt gttggcatca
     8761 gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg gaacaaaagg
     8821 gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt ttacctacag
     8881 tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg tcaaaagctg
     8941 cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt ttttcaaaag
     9001 caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc ggtaacttca
     9061 aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa ttaacttaca
     9121 agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa ctctaaacgc
     9181 cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat acttgaaaaa
     9241 aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca tatagaaagg
     9301 aattctccat cactgttcgg atattttttt atttttttcg gttactttct tctaatacat
     9361 ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa atcaggtagt
     9421 gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt cgctctaata
     9481 gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct actaatgccg
     9541 gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga ttaaccggta
     9601 gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct tctgttaatt
     9661 cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga atgattcctt
     9721 gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc caaaattctc
     9781 ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc ttacttaata
     9841 accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc tccttgacaa
     9901 atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta ttacatgttt
     9961 taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga ttttattgga
    10021 actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc caaaaatgac
    10081 catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt aaaagaccaa
    10141 gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac agagccaaca
    10201 gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg aatgatcttt
    10261 ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa cgaacatatc
    10321 gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact tgttcctgaa
    10381 tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt tattagcgaa
    10441 gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga tgaaaattat
    10501 aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg acctgagtat
    10561 tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc taataccgag
    10621 ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat gctttgagta
    10681 ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg cttattaccg
    10741 agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt ttgacctgag
    10801 tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg agctaatacc
    10861 gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata ttcacacagt
    10921 taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct gggagcatta
    10981 tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac aagttcctcc
    11041 tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga aagatagaaa
    11101 tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt ggtttctttt
    11161 atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt cacaaagatt
    11221 cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc tatttctgct
    11281 aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt ctcccaataa
    11341 gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga tagaacagat
    11401 tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct ccttacttat
    11461 tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt tcgcccctca
    11521 aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc aactttagga
    11581 tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt gactttccca
    11641 tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt tttcttatgt
    11701 ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat aataaatgaa
    11761 aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat aaacactaac
    11821 agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat ggttctttcc
    11881 tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg atgtcctgtc
    11941 actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt aactaagtct
    12001 tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg tatattttta
    12061 acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc gttttctaaa
    12121 cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga gatcactgaa
    12181 tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc tccattttca
    12241 aggatcctac cattgagccg atcataaagt gaaatattta attggatctt aggtatattg
    12301 atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt tctgttcttt
    12361 tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg ttttttctcc
    12421 tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac ttgttcgata
    12481 cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa agcgatcagt
    12541 aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg agactgtttt
    12601 tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt cttttattat
    12661 atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa gctgtcctgc
    12721 atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg ggttgggctt
    12781 attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa aactgcgtta
    12841 tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct aggctagtga
    12901 gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg aaaaagctac
    12961 tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac aaaacttatg
    13021 tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc ataaatattt
    13081 tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga atttatctaa
    13141 agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa tttttttcat
    13201 tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg gtgctgatgc
    13261 cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg gggatttcca
    13321 ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg ccgtttacgg
    13381 cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct ggtttcatta
    13441 tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc tgattttact
    13501 gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat tcatgagtac
    13561 gtattctatt caatactggt ctatcaacca aggattcact agctggggga cttgttttta
    13621 tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc ctgttatcaa
    13681 agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga tcatgttagc
    13741 tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa aaggaaaagc
    13801 actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa atatttgggc
    13861 aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta acagagaaat
    13921 gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta aggcctttcc
    13981 tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga acaccttatt
    14041 ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta gcttgattcc
    14101 atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt ttccatttcg
    14161 atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac tgaaccagtg
    14221 gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt tttcagcaca
    14281 gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat ttgtcagtcg
    14341 acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca gcaaagatat
    14401 gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa tctataagga
    14461 gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg aaacaacttt
    14521 tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg gggacaaaac
    14581 tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg gaaaaacatt
    14641 gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa aaggaatcaa
    14701 cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt ctcttacttt
    14761 gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc ttttacgtgc
    14821 ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat aatcgtttgg
    14881 agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt cgttcacatc
    14941 taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc catcaaaaat
    15001 ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta tacgcaattt
    15061 ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt ttcctttttc
    15121 attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag tataataacc
    15181 ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc gagtttccaa
    15241 aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat ctgtccacca
    15301 agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt tcccaatcaa
    15361 gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa ggacctgact
    15421 ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat aaccgatgtg
    15481 gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct gaactaaacg
    15541 gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga acttcattag
    15601 cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta aagcaatcac
    15661 ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct ctggttctgg
    15721 ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc ttgtcttcat
    15781 ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa atagtgttct
    15841 cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt ttggctgttt
    15901 ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta ttcaaactgc
    15961 tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt tgtttggctg
    16021 ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt taatggagcc
    16081 gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt ctactttatc
    16141 gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca catcaagatc
    16201 catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag caatctcaaa
    16261 agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt cgataccatc
    16321 tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt ttccagcgat
    16381 cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca tcattttttg
    16441 tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag ctgcggaaac
    16501 tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc ctgtagaaac
    16561 catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat ctgatttcaa
    16621 gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga tcaatcccat
    16681 cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat ttacacttac
    16741 gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc ccagcaaatg
    16801 aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata caacggtttc
    16861 ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga attttttgat
    16921 caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca acacgaagtt
    16981 gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca gcaattcgat
    17041 attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg cagccccttg
    17101 gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga actccaatgg
    17161 ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg atttatatct
    17221 tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca tcaagatacc
    17281 tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat gcaacacaaa
    17341 ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg cccaagccaa
    17401 ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata aaatctcata
    17461 cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca atccaaatgg
    17521 caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg cgctttcttg
    17581 tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg gccacaccac
    17641 agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa caaagcgctt
    17701 tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact tattataagc
    17761 cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca aagctggtgc
    17821 ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca gagtgtgggt
    17881 aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc cagtgatccg
    17941 attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt cttttgccca
    18001 acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg cccaaccaat
    18061 attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga tcaagggaat
    18121 tgatttccct atactgatca tcaacccagc tgccggcata actgctacca caaccattaa
    18181 agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc cgtttagctc
    18241 ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt tgtaaatgct
    18301 ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg tgttcccttc
    18361 tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata ttttcttttg
    18421 tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc tcattaggag
    18481 gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa tcagcacgac
    18541 acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg gaaacggtta
    18601 tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc aaggtactta
    18661 tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa atggttatcc
    18721 agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata tttcgatcaa
    18781 cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg aattagatat
    18841 gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata aagtacgcat
    18901 ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct atttaactat
    18961 agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg gaaatgttca
    19021 aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg gaactataga
    19081 agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg aacaatttac
    19141 tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg actatcacga
    19201 agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg agacatggtc
    19261 agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc agcaagcaga
    19321 aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa aagaagagca
    19381 aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag gagacgaaga
    19441 agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact acggcgaaga
    19501 tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg gagccactta
    19561 ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac ctgaagtaac
    19621 taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca acgctcagca
    19681 acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg aatgccataa
    19741 cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct atgcaatcta
    19801 caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac tggaagtttt
    19861 agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag caaataaata
    19921 catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata acaattggta
    19981 tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt taacttatca
    20041 aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag atatcgcaga
    20101 gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg acacattctt
    20161 agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc tgaatcagca
    20221 ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg tactacaggg
    20281 gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact ttgatttcta
    20341 tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga ttttagcagc
    20401 agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc gcttggattt
    20461 ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga ctggtagctg
    20521 gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt tgagattcgc
    20581 accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc gcggacgttt
    20641 attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag gttttcctct
    20701 agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa taacgctaag
    20761 aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc attaccgata
    20821 ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc attacgatcg
    20881 accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc tctttgcttt
    20941 tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa ttagcgaaaa
    21001 gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat gtcttccccg
    21061 gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct cttgcttctg
    21121 caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac tttgatgcta
    21181 ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc ctccttgcag
    21241 caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg caagccggta
    21301 tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct gaagaccttg
    21361 gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac ttgaaagaaa
    21421 agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga gactgaaata
    21481 aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta cggtgcccgc
    21541 ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt attgtcttac
    21601 gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt cggacctgta
    21661 gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa ctgcggatca
    21721 caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt agaagttttc
    21781 aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc tacctatcca
    21841 ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga gatgacaacc
    21901 tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta cttcaacctt
    21961 tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt agatggtgtg
    22021 cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga aaaactgcca
    22081 atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc agatggacta
    22141 gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca attattcgag
    22201 aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt attattttct
    22261 acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc aaactacgga
    22321 ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt tggatcgatc
    22381 aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc tgcacaataa
    22441 caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa aaaagccggt
    22501 gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc cgagacaaca
    22561 aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat gactgcccgc
    22621 aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct tgattcaaaa
    22681 gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga agaagcgcgg
    22741 aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct gttagagaac
    22801 gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta ttcagatagt
    22861 caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt gatcggacag
    22921 ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct tctaggtaaa
    22981 caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt gacaaacacg
    23041 actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat gatgatggct
    23101 ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct gtcctttgag
    23161 aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat cttcgccctt
    23221 cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact ctccgtcatt
    23281 agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag tatcgatatc
    23341 gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat ccatgaagaa
    23401 ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga gacagtcgtt
    23461 cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat ttatcctatg
    23521 gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct gtaaaaaaga
    23581 gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga
    23641 agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa agcaattttc
    23701 ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt ttttccttcc
    23761 ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta aagacaggtg
    23821 caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc atcgttccca
    23881 taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca cctgaatccg
    23941 ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa cgcttgaatg
    24001 tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg attccgatac
    24061 agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct tttaattctt
    24121 gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc aatgcaagta
    24181 aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc agttcatcta
    24241 gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct gcttttccga
    24301 ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat cccgctagta
    24361 atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact agtggaatca
    24421 atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca ttgatactag
    24481 ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac gaaaggacga
    24541 atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta actcctccaa
    24601 tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct aaaaaggcac
    24661 cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga ccgatcccca
    24721 aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc actccgacaa
    24781 ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt ttaaatccat
    24841 aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag acaccaatca
    24901 aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc ccgtcatgga
    24961 tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg aaagctgcaa
    25021 ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg agtgccaaca
    25081 acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc gtcgatctcc
    25141 tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga cctcctacta
    25201 aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc taaacttttt
    25261 tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg aataacgaac
    25321 atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt acctaatgta
    25381 gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca agctaatccg
    25441 tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt acgcatttta
    25501 agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt agcatttgta
    25561 agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc aaataatgaa
    25621 ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt ttctttatgg
    25681 aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt ttttgatggt
    25741 ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa gctgaagata
    25801 tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg ctgcgtgata
    25861 tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc attgatgaca
    25921 ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt ttttgttgcg
    25981 tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat aaatgtgata
    26041 ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt tgaccatgaa
    26101 aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa aacacctaaa
    26161 acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt cccgccgact
    26221 tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac taaaatacaa
    26281 cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt tcctaacaat
    26341 ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca taatagccat
    26401 gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg aggaccgcct
    26461 tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc ggcaaaaaac
    26521 aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa cgtaggaata
    26581 ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag acccatttta
    26641 cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa aataacggtg
    26701 tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag gggattcttt
    26761 gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga tcgaagagct
    26821 taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt attcaagctg
    26881 ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg tctgcgtaga
    26941 ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct tcaaagtgtt
    27001 cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact tttccggcac
    27061 ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg ctgttgccat
    27121 agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa atgcttcctt
    27181 tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca actaagtcat
    27241 cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa gttaatggat
    27301 gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag ttgtagaaac
    27361 gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca ttcaaaggta
    27421 agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa ccttccaaga
    27481 tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat gtcgcaaagt
    27541 aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca gaatatggaa
    27601 catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt gctttatatg
    27661 cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg tatttgtaat
    27721 gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag aaaatcgcac
    27781 ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg tctttttcta
    27841 atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct tctgttgatc
    27901 ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca cgaacgattt
    27961 tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga atcacttggt
    28021 ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta gtttgtggaa
    28081 ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga gctttgattt
    28141 cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt gacaagttca
    28201 ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc attgcaagcg
    28261 gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa gaaccatcag
    28321 caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc attcctactt
    28381 cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta ccattccata
    28441 gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc atatgacccc
    28501 agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct gaaatttctg
    28561 atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt ttttcttctg
    28621 gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa tgttcatcca
    28681 ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc ccaatgaata
    28741 gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc tcctaaaaaa
    28801 atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta aactaagttt
    28861 tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt cacacttttg
    28921 aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa gattgtatta
    28981 acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt aatagtattt
    29041 ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca aaaagtaatt
    29101 tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg gatttatgac
    29161 aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg aaacatcttc
    29221 acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca cgatattttc
    29281 aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga tttcttccgc
    29341 atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg cttctttcaa
    29401 gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt gtgcttcacg
    29461 tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt gagacaagaa
    29521 agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc agaattggta
    29581 gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg cagtttttcc
    29641 gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg cttctacttc
    29701 tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc attggtctgc
    29761 tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt cgatagattg
    29821 cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc tggcaacgat
    29881 atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca aaaattcaag
    29941 taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg catctttacc
    30001 aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt gttccatcgt
    30061 ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg ttccgccgcc
    30121 aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca taaatggaat
    30181 caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa gtgaaatgct
    30241 cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa tggcgtcgcg
    30301 ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt tttatgaaag
    30361 caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg aaataaaaaa
    30421 agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat ttgtatgtct
    30481 agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac tgccaaagac
    30541 tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc agctttctga
    30601 aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt aatatgtgtc
    30661 tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt taaattcctc
    30721 attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt attgttaaaa
    30781 gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta ctttgcctct
    30841 tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa gcaagggacg
    30901 aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt aactatcgtt
    30961 attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga accaatcatt
    31021 cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca acaactagtt
    31081 actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc gttattggaa
    31141 aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact aaaaaaggag
    31201 aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt tttccacagc
    31261 ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata caaagattat
    31321 atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa tgattttgtt
    31381 ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc ctttaacggg
    31441 cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt cagctattat
    31501 gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa caatgaaatc
    31561 aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat ggttcctctg
    31621 atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa aggtaccttg
    31681 gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca atatggagac
    31741 aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca gttgaagaaa
    31801 gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca agcttcacta
    31861 gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat cctaggaaaa
    31921 acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc ttattcagca
    31981 gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg taatgtcgat
    32041 gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc tcggatcaat
    32101 atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct tgatccattc
    32161 atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt ttacgtcatt
    32221 tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt tatgctttat
    32281 ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt atatggaagc
    32341 agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa atagaggatc
    32401 aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg atggaaaaga
    32461 tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga aaattctttt
    32521 ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt cactcgattg
    32581 agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg aagattttcc
    32641 gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa tacttcataa
    32701 atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac ctgagcgttc
    32761 tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat caagtcaaaa
    32821 gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc gttcatttgt
    32881 agagcc
//
LOCUS       DARDHA010000014        31583 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000014 DARDHA010000000
VERSION     DARDHA010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.014"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..584)
                     /locus_tag="SDA10_000558"
     CDS             complement(<1..584)
                     /locus_tag="SDA10_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG4239488.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQ"
     gene            complement(660..806)
                     /locus_tag="SDA10_000559"
     CDS             complement(660..806)
                     /locus_tag="SDA10_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HEG4239489.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(910..1221)
                     /locus_tag="SDA10_000560"
     CDS             complement(910..1221)
                     /locus_tag="SDA10_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG4239490.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(1223..1420)
                     /locus_tag="SDA10_000561"
     CDS             complement(1223..1420)
                     /locus_tag="SDA10_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HEG4239491.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1814..3541)
                     /locus_tag="SDA10_000562"
     CDS             complement(1814..3541)
                     /locus_tag="SDA10_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEG4239492.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(3534..4727)
                     /locus_tag="SDA10_000563"
     CDS             complement(3534..4727)
                     /locus_tag="SDA10_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG4239493.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4914..5558)
                     /locus_tag="SDA10_000564"
     CDS             complement(4914..5558)
                     /locus_tag="SDA10_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4239494.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(5652..5786)
                     /locus_tag="SDA10_000565"
     CDS             complement(5652..5786)
                     /locus_tag="SDA10_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEG4239495.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5788..7920)
                     /gene="feoB"
                     /locus_tag="SDA10_000566"
     CDS             complement(5788..7920)
                     /gene="feoB"
                     /locus_tag="SDA10_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEG4239496.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7917..8390)
                     /locus_tag="SDA10_000567"
     CDS             complement(7917..8390)
                     /locus_tag="SDA10_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEG4239497.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8791..9015
                     /gene="nrdH"
                     /locus_tag="SDA10_000568"
     CDS             8791..9015
                     /gene="nrdH"
                     /locus_tag="SDA10_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEG4239498.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            9174..11333
                     /gene="nrdE"
                     /locus_tag="SDA10_000569"
     CDS             9174..11333
                     /gene="nrdE"
                     /locus_tag="SDA10_000569"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEG4239499.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            11383..12348
                     /gene="nrdF"
                     /locus_tag="SDA10_000570"
     CDS             11383..12348
                     /gene="nrdF"
                     /locus_tag="SDA10_000570"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEG4239500.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(12437..13576)
                     /locus_tag="SDA10_000571"
     CDS             complement(12437..13576)
                     /locus_tag="SDA10_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG4239501.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13885..14598)
                     /locus_tag="SDA10_000572"
     CDS             complement(13885..14598)
                     /locus_tag="SDA10_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEG4239502.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14852..15553)
                     /gene="nagB"
                     /locus_tag="SDA10_000573"
     CDS             complement(14852..15553)
                     /gene="nagB"
                     /locus_tag="SDA10_000573"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEG4239503.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15787..17319
                     /locus_tag="SDA10_000574"
     CDS             15787..17319
                     /locus_tag="SDA10_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG4239504.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(17510..18712)
                     /locus_tag="SDA10_000575"
     CDS             complement(17510..18712)
                     /locus_tag="SDA10_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4239505.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(18794..19483)
                     /locus_tag="SDA10_000576"
     CDS             complement(18794..19483)
                     /locus_tag="SDA10_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG4239506.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(19589..19957)
                     /gene="rplL"
                     /locus_tag="SDA10_000577"
     CDS             complement(19589..19957)
                     /gene="rplL"
                     /locus_tag="SDA10_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEG4239507.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(20010..20513)
                     /locus_tag="SDA10_000578"
     CDS             complement(20010..20513)
                     /locus_tag="SDA10_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEG4239508.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(20717..20844)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(20923..21612)
                     /gene="rplA"
                     /locus_tag="SDA10_000579"
     CDS             complement(20923..21612)
                     /gene="rplA"
                     /locus_tag="SDA10_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEG4239509.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(21716..22138)
                     /gene="rplK"
                     /locus_tag="SDA10_000580"
     CDS             complement(21716..22138)
                     /gene="rplK"
                     /locus_tag="SDA10_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEG4239510.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            22481..23149
                     /gene="sdaAB"
                     /locus_tag="SDA10_000581"
     CDS             22481..23149
                     /gene="sdaAB"
                     /locus_tag="SDA10_000581"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEG4239511.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            23162..24034
                     /gene="sdaAA"
                     /locus_tag="SDA10_000582"
     CDS             23162..24034
                     /gene="sdaAA"
                     /locus_tag="SDA10_000582"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEG4239512.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(24125..25144)
                     /locus_tag="SDA10_000583"
     CDS             complement(24125..25144)
                     /locus_tag="SDA10_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEG4239513.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(25317..26222)
                     /locus_tag="SDA10_000584"
     CDS             complement(25317..26222)
                     /locus_tag="SDA10_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG4239514.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(26281..26826)
                     /gene="nusG"
                     /locus_tag="SDA10_000585"
     CDS             complement(26281..26826)
                     /gene="nusG"
                     /locus_tag="SDA10_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEG4239515.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(26927..27097)
                     /gene="secE"
                     /locus_tag="SDA10_000586"
     CDS             complement(26927..27097)
                     /gene="secE"
                     /locus_tag="SDA10_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEG4239516.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(27121..27273)
                     /gene="rpmG"
                     /locus_tag="SDA10_000587"
     CDS             complement(27121..27273)
                     /gene="rpmG"
                     /locus_tag="SDA10_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG4239517.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            27435..28079
                     /locus_tag="SDA10_000588"
     CDS             27435..28079
                     /locus_tag="SDA10_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG4239518.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(28135..28638)
                     /locus_tag="SDA10_000589"
     CDS             complement(28135..28638)
                     /locus_tag="SDA10_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEG4239519.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(28651..28697)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            29235..30200
                     /locus_tag="SDA10_000590"
     CDS             29235..30200
                     /locus_tag="SDA10_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEG4239520.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(30299..31471)
                     /locus_tag="SDA10_000591"
     CDS             complement(30299..31471)
                     /locus_tag="SDA10_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4239521.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(31458..>31583)
                     /locus_tag="SDA10_000592"
     CDS             complement(31458..>31583)
                     /locus_tag="SDA10_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4239522.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         9726 a         6437 c         5517 g         9903 t
ORIGIN      
        1 tattgttctc gttgcctaat agttattcta ataaataaaa atagaacaac aaacatttga
       61 atgattaaaa atattaagac accaaacaca aaatgatcaa atacttgata ttgatgagca
      121 acataagaaa caaataaacc aattgtaagt aaataaatca tgaatatagc agtaagttta
      181 gaactggact gttcaacaat cctatttata gctaattttc tatataacca aataaacatc
      241 agcaaaaaca aagaatcaag cattatctgg aatactacat aaccataccc ttttatccct
      301 tcaaatgaaa acatatacgt cataaccatg gaagtaataa tagatctgaa atcatcaata
      361 agcatacata atagtaattt atttaaaagc actatattag ttttcttctt ttcctttaat
      421 ataaaataac ttccgattat aggtatagct gcggaaacat cagtaaataa ttcaacaaaa
      481 aaagtgagtg gtatcaataa acatacaaaa agatattttc ttttgttaag atcagtatta
      541 gtcatataaa ataagagaaa aaagaaaaag tttcccacga acattcttaa tagataagct
      601 tttgtatcga tagtaatcaa gagatcccct cctgatttta ggatatcata aaaaaaattt
      661 tagttacact ttttaaataa tgaaaaaaca cattccacta aatttgatgc cggttttcct
      721 tgaggttttg ttccagcacc tcctttaatt tttttaagct cttgatctga acattgttta
      781 gcatttagtc tatttttttc ttccattacg attcccccta caaactataa atacttttat
      841 gtcactgatt caatatacct atttttctaa tcaaaaaatt ttgagacata tttaatatta
      901 taagtatcat taaaattgag atttatctcc ataatctgct cgatataaac catttattcc
      961 agcagttctt ccaatttcac tcaaatttct tattaattcc tcttgatatt cagtgaatct
     1021 tattttatta gtataagcag taaaatatag ataattcacc aatctattta ttaaaggacc
     1081 gggatctaat tcatattttt gtttttctaa ttttaaatat accttttgta aaacctcaag
     1141 aatatcagaa tatttaggat ctttacttat agatatatca ttatataatt catgaacaat
     1201 ttgcttagca ttttttttca ttttagcact tccctggaat tgctccacct aaaaaaccac
     1261 ctatagacat tcctgcaata caagtagttg ccttggccca atcgaccgta catttatttt
     1321 tagtgcaata cactccattt ccataatatt ttccactatg agtggtaccc ccatagataa
     1381 gttgtgtctc tttaatagac aaaattttta aatgtttcat aatctaatca cccctaaatt
     1441 aatgttttta gactattatt ctaacattca ttaaatttca aaaaacaatt ccttcttgaa
     1501 acgtagcaaa ataattcctg aataagaatt taatcacttt taagtatata aactatgtat
     1561 attctttaat attttttgag aatgcacttc gtacataata aactttaatc acattagtct
     1621 ttcataccta cgatcgatct ctttgactcg cacccataaa ttcataatag tttgtcggcg
     1681 tctttggacg tttcgaagat gagctacgca ttctcttttt ttatagacaa aaaagataga
     1741 gacgaacatg gagcttccaa atttggatat ttgatccaat tttggaagct ccatttattt
     1801 cgtgtctgtc tttttatttt ttccagcttt cgactaattc ttggttctct ttgatccagt
     1861 cttttgctgc ttgctgtgga tcttttccat tgttgatttc caacataact ttttccatat
     1921 ctttttctga ccaatggaaa ttgtctaata ctttgtaagc ttctggctgg tcttctttca
     1981 atccttttcg aaccatcgta tggatctgct cttcgcttcc cattccgttt tcaggatctt
     2041 ctaagtattt cagatcatat ttggcaaaca tccaatgagg tgtccatcct gtaactacaa
     2101 ttggttcgtg ttttttgatc gcttgaccta gtgcaacggt cattgctcct gaagaagatg
     2161 tctctacttt ccagtctttc aagttgtcat atttttggat cgtgttttca gcagccgtaa
     2221 ctacaccagc tcctggctcg attcctatga ttttcttacc tgcctgatcg gttaactcat
     2281 cgatcgaatt tacatccatg taggctggca ctgctaaacc aacttttgct cctgtcaaat
     2341 tggcacctag gtcttcgact tgatctttat attgtgcata ttgtgaacca tgagttttag
     2401 gtagccaagc tgaaaccatg gcatctgact cgccatttga aacagatttc cacatgattg
     2461 aattatctaa tggcgtcatc gttacatcat agcccatttg tttcaatact tcaccgacta
     2521 catttgttga agcaacttct gtatcccatt caacataaga taagttgata cgttttgttt
     2581 ctttggcaga tgaaaaactc cctataccaa tcgcacctaa gatcactact actacagcac
     2641 caatgatcag tccttgacgt tttttacttt tctgtgttgt tgctcctgca gcttttttat
     2701 tgtttggctg gttcaaatgc tgtgtaaaac ggtcaatgat gattgctaag ataactaatg
     2761 caaccccgtt gacaaatcca ttcccgactt gtgctctttg caatgctgat aatacgccgc
     2821 gtcctaagcc gggtgcaccg atcatagagg caataacgac cattgaaagt gacagcattg
     2881 ttgtttgatt gatccctgcc atgatcgtac ttttagctaa aggcaattct agtttgaata
     2941 gtttttgacg acttgtgctc ccgaatgaat cagaagcttc tactaattct ttaggaactt
     3001 gtcgaatccc taaattcgta aaacgaacgg ttggtggcag agcaaagatg actgaggcaa
     3061 atacccctgg aaccatcccg ataccgaaaa atgcaactgc tggaatcaaa taaacgaatc
     3121 ctggcattgt ctgcataaag tcaaggatcg gtttgataat actttgggct ttactgcttt
     3181 ttgccatcaa gatccctaat ggtaccccaa tgatgatcga gatcacacta gataaaagaa
     3241 caagtgtgac agtattcatc aaatcattcc atagaccttg gttataaata aaccatagac
     3301 cgattaatgt gaacaacgta agtcctggac gtttcttaga aataaagaaa gctgctaccg
     3361 tcaataacaa gataaacaaa ggagctggaa tcactaacag taaatttgta atccccgaca
     3421 tcactgcctg tccaatcgtt tgtaaaaatg aaaataggcc agaaaaagtt gtcgtaaacc
     3481 attctgtgat attttctacc cagtttgcca ctggtaattg atagttcaat agattattca
     3541 tgttcactca cctcgcttgt ttctgctaat gcttcaatca cactacctcg gatgacgaca
     3601 ccttttaatt ttccatcctc tacaactgca agtggagtcg gtgagtcata aataatattg
     3661 aaaatatcac ttaccaccat gtctttccca atcaccgtca cattttcatc taggaagtcg
     3721 atcaatggac gtttttgctt cctagcttct aacgcttgat ctgcagtgat gatccctttc
     3781 aggtgtctct ttctgtcaac agccatcagc atgctgactt cttcattacg catccttgtc
     3841 aatgcaacgt ttggtccatc agattcgata ttcgtcgtca atgcaggtac catgatgttt
     3901 tgagccgtaa gcactttgga acgatcgact tcttccacaa attcacgtac ataatcattt
     3961 gctggattgg tcaatatttc ttcacctgtt ccgatctgca tgatttcgcc gtctttcatc
     4021 aaggcgattc tgtccccaat ccgtaaagct tcattcaaat catgggtaat gaaaataatc
     4081 gttttttgaa cattggcttg caggtccagt aattcgtctt gcatttctcg ccggatcagc
     4141 ggatcaagag ccgagaacgc ttcgtccatc agcaagatct caggatcatt tgctaaagct
     4201 ctggctaggc caacccgttg ttgcatccca ccagataact gactaggata ttggtcttta
     4261 aatgaaagca aactagaatt ttctaatgct ttttctgctt tttcttgtcg ttcttcttta
     4321 ggtacaccac ggacttctaa cccgtattct gtattttcca agatcgttcg atgtggaaac
     4381 agaccgaagt tttggaaaac catattgatt ttatgacgac ggacttctcg caattcttct
     4441 ttgcttaact tcgatacatc ttctccatca atatagatac tgcccgaagt tggctcaatc
     4501 agacgattca atagacggat caatgtggat ttcccactcc cggaaagccc catgatcacg
     4561 aagatttctc cttcttttac gtcaaaattc acgtcataca caccaaccgt tgcaccggtt
     4621 ttctttaaaa tctccgtttt cggctggtga tcattcatca tatctaaagc ggcttgtgtc
     4681 tttttaccaa atatctttgt taaatgtttt acttctactt ttaccaaaca aattctcctt
     4741 ttttatgtaa attcattttt ctttctgaaa aaagtgacca cactatgtta acatcatgta
     4801 gtcactttgt caaaaatgaa agaaatttgc ttgtccaaac tgaaaaaaac ttggctaaat
     4861 ctttgtaaat aaaaggttta gccaagttaa tattttttta atatttcaca caatcattct
     4921 gcaagctgat gctccgggta aaaaaagttt gtcatacgct gtagtgtata ttcatttcca
     4981 ataaaatgaa cgatgtcccc tgccgtaaat ttagcatagg ggccaggtga aacaagcagt
     5041 tcttcattat gagaaattgc gataactgtg gcgccagtcg cctgccagat attcaactcg
     5101 ctaattgaac ggtccagatg cattgctttt tctgataaag ataattcaaa cggaagtaaa
     5161 ggattcatct tatgtattcg cttcgtttga ttgaccagct gatccaataa ttgagaaaaa
     5221 tgattcagct catcttgttg cttctccaca cttgccatga tttctgcttt tgtatcttgg
     5281 atggattgta catcttgata ttgcaatagg aaatcttttg ctttttcctt cgaagcaaca
     5341 aatgccccac ttccatgccg tacttccatg attccaagat ctaccagcac attgatagcc
     5401 ttgcgggtag tctcaggaga agcattgtac gtactggcaa gtgttgagcg ggcatggatc
     5461 ttctcaccca ctttatatcg gttttcaacg atacgttcgg ctaaatcaac agcaatctgc
     5521 tggtagcgcg ggcggacgat ttttttcttt ccagacattt tctttcctct ttcttttttt
     5581 actttccctc atcttactat gctctagcaa aataagcaaa aaaggggaga atagcctctt
     5641 gcaacgactg atcatcgctt gatctgttcc ttttttactg ggcaagctgt ttgacagtca
     5701 tcacaattct taccggattt taaacgagta tagacaattg cgcctgcact tccaaagatt
     5761 aacacactta ataagaatgt cgccatccta ttcctctcct tctaatgaca atatagtata
     5821 aaactggttc ttggattttg gctgacggaa caaaacataa acgagtccta tcatcagtaa
     5881 aatagcaaga tacgtcccta acgctacctg tccaccctcc aataagtgtc ccaactgata
     5941 aatcacaaaa ctaactaaat aagctaaacc gcattgatag cctatggcga tccaagtcca
     6001 tttcttggca ttcatttccc gatggatagc cccaattgcc gcaaaacaag gcgcacaaag
     6061 taaattgaag gctaacaatg aataagctgc tgcgggagta tatgtgtgtt gtagcacttg
     6121 ccagatttct ctgccatttt cggatacttc cgcatgacca aacaaaatac caaatgtgcc
     6181 aataatattt tctttagcga ccaatcctgt aatcgctgcg actgttcctt gccagtttcc
     6241 ccaaccaagc ggctgaaaaa caactgccag gattcgcccg aaaaaggcta agatactttg
     6301 gtcttcagaa acaggttgag cataaaaatt ataagaagag ctgaaccaga tcacgatact
     6361 agaaacaaag ataatcgttc ctgctttttt aacaaaagac ttgctgcggt cataggtttg
     6421 tcttaggacc gctgaccatt gaggaaaatg atatgcgggc aattccatga taaaaggagc
     6481 tgtatctcca gcaaacaaac gtgttttttt caagctgatt ccagagaaaa taattgcagt
     6541 cataccaaga aaataggcag aaggagctac ccaagattga tgaggaaaaa aggcccctgc
     6601 tactaaggca ataatcggca actttgcaga acaaggcatg aaggttgtga ccatgatcgt
     6661 caaacgacga tcttgctcgt tttcaatcgt gcgactggcc atgacgcctg gcactccaca
     6721 tcctgtagca atcagcatag gaatgaagga ttttcctgaa agaccgaatt tcctgaaaag
     6781 tcgatccatg acaaaagcga tcctcgccat atatccgcag tcttccagaa agctcaagca
     6841 taaaaacaat ataatcaatt gcggtagaaa tcctaacact gctcccacgc ctgcgatgat
     6901 gccgttcaag atcagctcct gcatccatcc cgctacttgc cattcagcta acaatctgcc
     6961 aacatgttct ggtaaccatg taccaaacaa tgtgtcattg atccaatctg tccccattgt
     7021 tcctactgtt tgaatagcca gataatagat cagccacatc acaaatgcga agatcggcaa
     7081 ggctaaccaa cgattcgttg caatctgatc gattttgtca ctcatcgata attggaaaga
     7141 atcatttgac acaacacaga gtgtacatag ttgggtaata aattcataac gttcattgac
     7201 cagaatcgtc tcactatcgt cgtccaatag tttctctgtt aactggatca gttgctcgat
     7261 ctctttttgc tgagaaactg ctatctcgta atcctctctt accttctgat ctcgttcaaa
     7321 atatttcatt gcataccagc gtttcttgcc gctttgttct cctagtacat cttcaatttc
     7381 atgtaaagct gcttctaaac gtggatcata tgtacaggga acaggaatag aaggccgctt
     7441 ttttgcagac tcctgaatct tttcgattag tttttcgatt ccttgctttt tcaatacgct
     7501 gattggtaaa actggtacac caagaccata ggaaagcttt tctagattga ttttttttcc
     7561 ttcttttttg atgacatcca tcatattcag accaatgatc ataggaatgt ctaaatccaa
     7621 taactgcgta gttaaataaa gatttcgttc taaattggta gcatcaataa tgttgatgat
     7681 cagttcaggc tgttcctgaa ccaaataatc tcttgcaatc acttcttctg gtgtataagg
     7741 agacaaagaa tagatgccgg gtaggtcctg aatatgaatg ttcggatttt ttttcaacga
     7801 tccttctttt ttttctactg ttacgcccgg ccaattccct acatattgat tcgaaccagt
     7861 caagagattg aacaagctag ttttcccgct gtttggattt ccgactaacg cgatattcat
     7921 tctttttcct ccctttgtat caagattagt tcggcctctg actttcgtaa cgtcagttca
     7981 taccctcgca ctgtcagttc gattggatct cccagagggg ctagttttcg gataactatt
     8041 tttacatttc gcgtcaatcc catatccatt aaccgtcgct tcactgcgcc ttgtccgtgg
     8101 attgctacga cgatcccagt ctctcctgct tttaattgat caagagaagt ccagctttca
     8161 gaggaagcag tctttttatt gacaaagatt tgacctagta tttcccgact tactgccact
     8221 cgatgacttt ttaccagtaa aatggcattg ttgcctgata aattgacgac gacgatttta
     8281 cttccggcaa cgatcccgat attttgcaaa tggcgttttg ttttttcttc tgtttcgatt
     8341 cgttcgactt gataaacgaa attctgcttt gcctgcgcta aggtctgcat attgattcct
     8401 ctcttgtgaa ataaatcatg tattaaaatc attcattata tgttctaatt ttcttacgtg
     8461 ttacgcaaat aaatgagaat cattctcaca tagaatactc tcggtaattg agaatgtcaa
     8521 ttatacttgt gatattatga gcgaatcttt agtattgata gataaaaatg agaaaaaaat
     8581 atttcttcat agttttccac aattttttct ctattaaatt tgctattttc ccacacgata
     8641 cttgttttca gctctttttt caaaaacaca aaacttggta ttccacagtt tccacaacac
     8701 aatatgtagt gtttgttgtt tgactttttt actaagagtt ttatactatc tattatagat
     8761 aataagaatg tgttagagga ggcactcacc atgaacgtaa aaatcttttc taaaaataat
     8821 tgtatgcaat gtaagatggt caaacggttt ttaacagaaa atcaaattgc ttttgaagaa
     8881 gtcaatatcg atgaacagcc tgaagcagta gactggttga aagcacaagg attccaaagt
     8941 gttcctgtca ttacttctga tgcagaaaca gttatcggat tccgtcctga tcagttgcgt
     9001 aaacttgcca gctaacatga ctaaaagaat caaaacgtga tacatggatt tgaagggctt
     9061 gagacagcac cagtttttgt cgcaaacttc caaatccttt tttaaagctt aaaaacaatg
     9121 caatgatttg caatcaagca tcatcaaata cgaaatgaag gttgggcgta cgtatgagtc
     9181 ttaaaaattt acaagatgtc agttatttca agctgaataa tgaaatcaat cggccagtca
     9241 atggtcaaat ccctttgaat aaggataaag aagctttagt tgcctttttt gaggaaaatg
     9301 tcaaaccaaa tacgatgact tttctatcta ttatggaaaa aatcaattac ttgatcgatc
     9361 aagaatatat agaagcagaa ttcattcaat tgtattcacc atcatttatt gaaaagctat
     9421 atcaattttt ggacgagcaa catttttctt tcaaatcttt tatggctgct tacaaatttt
     9481 attctcaata tgcgttgaaa accaacgacg gttccgctta tctggaaact tatgaagatc
     9541 gagtagcctt caatgcgctt tactttgcga acggggatga agaattagca cttgctcttg
     9601 cagacgaaat gatccatcaa cgatatcagc cagctacacc ttctttcttg aatgctggac
     9661 gtaaacgtcg gggcgaattg gtgtcttgtt tcttgactca agtcactgac gatatgaact
     9721 ctatcgggcg ctcaatcaac tctgctttgc aattgtcccg tattggtggc ggcgtaggga
     9781 ttactttatc aaatcttcgt gaagccggcg ctcctatcaa aggatatgag ggtgctgcaa
     9841 gtggtgtcgt tccagtcatg aaattattcg aagatagttt cagctattct aatcaattag
     9901 gtcagcgtca aggagctggc gtggtttatt tgaacgtttt ccatccagac attattgctt
     9961 tcctttcagc aaaaaaagaa aatgcagatg aaaagattcg cgtaaaaact ctttctcttg
    10021 gcgtgatcgt acctgataaa ttttatgaat tgacacgtaa aaacgaagat atgtatttat
    10081 ttagtccata tagtgtcgaa aaagaatatg gtgtcccatt ctcttatgta aatattacag
    10141 ctgagtatga tcatctagtt gcaaatccaa acattcgcaa acaaaaaatc aaagcgcgtg
    10201 atcttgaaaa tgaaatctca aaattacaac aagaatctgg ctatccttac gtaatcaata
    10261 ttgatacagc gaaccgcgag aatccaatcg atggcaagat catcatgagt aatttatgtt
    10321 cagagatttt acaagttcaa acgccttctg tgattaacgg caaacaggaa tatgaagtat
    10381 taggaacgga tatctcatgt aacttaggct cgacaaatat cgtgaatttg atgcaaagcc
    10441 ctaactttgg tcaatctgtg cgtacgatga ctcgtgcatt gacatttatt acagatgcat
    10501 ctgatatcga tgtcgtacca ccgatccaaa acggaaataa gctgaatcat acgattggat
    10561 taggtgccat gggcttacac acttatcttg caaaagaaca aatggaatat ggctcagagg
    10621 attcattaga ctttacaaat atttacttca tgctgttgaa ttattggaca cttgttgaaa
    10681 gcaacaatat cgctcgtgaa agaaaacaag ttttccataa ttttgaaaaa tcagcttatg
    10741 ctgacggcac ttatttcgaa aaatacgtaa ctggagaatt ccagccaaaa tcagaaaaag
    10801 tcaaagcatt gtttgaaggc atttttatcc caactgctga agattggaat gctttgaaac
    10861 aagcagtaat gaaagatgga ctgtatcatc aaaatcgctt ggcagtagca ccaaatggct
    10921 caatttcata tatcaacgat acgagtgcaa gtattcatcc tatcactcgc atgatcgaag
    10981 aacgccaaga gaagaaaatc ggtaaaattt attacccagc gccttatcta tccaatgaga
    11041 cgattccgta ttacacttct gcttatgata tggacatgcg taaagtaatc gatgtttatg
    11101 caatggcaca acaacacgtc gatcaaggca tgagtttgac tttattcatg cgttctgaaa
    11161 tcccagaagg catttatgaa tggaagacaa caacgaagca aacgacacgt gatttgaata
    11221 ttttaagaca ttacgctttc cacaaaggaa tcaaatccat ttattacgta cgtaccttta
    11281 ctgatgatgc agaagaaatc ggaagcaatc aatgtgaaag ctgcgtgatc taatatcata
    11341 gatagattcg cttattcata ttttaattaa aggagcattc tcatgtcaga aacatattat
    11401 gaagcaataa actggaatga aattgaagat atcattgata aatccacttg ggagaagctg
    11461 acagagcaat tttggctaga tacacgtatc ccactttcaa atgacctaga tgattggcgt
    11521 acattgtctc aattggaaaa agatacagta ggacatgtat ttggcggttt gacactgcta
    11581 gatactgtcc aatctgaaag tggcatggat cagttgcgta aagatgtccg cactccccat
    11641 gaagaagccg ttttgaacaa tattcaattc atggaatccg ttcatgccaa aagttattct
    11701 tctattttta gtacactgaa tacgaaaaaa gaaatcgaag aaatttttga ctggacaaat
    11761 acgaatcctt atctgcaaaa aaaagctgag cgtatcaatg aaatctacaa acacggtaca
    11821 ccgctagaaa agaaaattgc cagcgtcttt ttagaaacat tcttgttcta ttcaggtttt
    11881 tatactcctc tttattattt aggaaacaac aaattggcta acgtagcaga gatcatcaaa
    11941 ctaatcatcc gagatgaatc tgtacatgga acctacatcg gttacaaatt ccaattagga
    12001 ttcaacgagt tacctgaagc agaacaagaa acgttaaaag actggatgta caatcttctt
    12061 tatgagcttt acgaaaacga agaacgttat acagaagaat tatacgatcc aatcggctgg
    12121 acagaagaag tcaagacatt cctacgctat aatgccaaca aagcattgat gaacctaggg
    12181 atggatccac tctttccaga tactgccaat gatgtcaacc caatcgtaat gaacgggatt
    12241 tcaacaggca caagcaacca tgacttcttc tcacaagtcg gaaacggcta tttgttaggt
    12301 catgtagaag cgatgaaaga cgacgattat ttgattggat tggaataagg atttatttta
    12361 attcaaaaat ttttcgaaaa ataaaaagag tttagcgtac agatatttta aatgtacact
    12421 gaactctttt ttatgcttat tcacttgttt cttccagcac ttttggactg tctaactttt
    12481 ccgaaacatg ttccattctt ctcaataaat agattttccg gatatttaat attatcgtct
    12541 taatcactgc atagacaggt atgcaaatca gcatgcctaa tattcctgcg acacttcctg
    12601 ctccaatcaa taccaagatg atcgtcaaag gatggatatt cattgatttc ccaaataaca
    12661 acggcttgat caaatttcca tctaattgtt ggatcactaa aatcgacaac gccatataca
    12721 aagcttgaaa tggagagata aacaaaccga caatgattgc tggtaccgct ccaataaaag
    12781 ggccgacata cgggataata ttcgtgattc cgcaaatgac cgcaagcaac aagctataag
    12841 gctgatcgaa tattctcatt gccaaaaagc tcattacacc aataatcaaa gcatctaaga
    12901 tcgtactgct gatataggca gataatgttt cgtttaattc ttttaccgtt tggcgtaact
    12961 ctttacgaat cgctttcgga aagaagtgag acaacgcatc aataaatttg tgcccatcct
    13021 tgaacataaa tagcaaaata aatggaacgg taaataacaa aataaaaaat tgcatcataa
    13081 atgaaatgat ttttgagaca cttgcagata aactgactaa tatgatattg aacaaattac
    13141 taagagataa atttgctgat gctaattgtt cttccaaatt gaaattttta aactcttccc
    13201 gctgcgccaa ttcatttaac catttgcttg tttcatctgc atacattggc aagctctggg
    13261 tcaattgaat tgtctgttcc attaactgag gcactacatt catgacagtt aaaacaacta
    13321 aaatgattac gaccaaaaac gtcaataaaa aaccgaacac tctcgggatc cgatgctttt
    13381 cgagaaaaac aacgattgga tcgaacatat aatagataaa accagcaaca agcaatggta
    13441 aaaaaactgc cgaaagcatt ttcaatattg gactaaaaat attcggcatt tgtaaaataa
    13501 agtacacgcc gatgatcgtg agtactagct ccactgtcca gaaaaataat tttgattgtt
    13561 taaaacgtga ttgcatacat ctcctccaag gtcctttgat acttctattc tatcatttaa
    13621 caaagagtta cgcgctgaga ttttcatttg cttaaaaaaa acaaaaaaaa taaacatatt
    13681 ttaatgagta gacgagtact taaacaaaaa cacggtttgg agtagtgaca aaaattatac
    13741 tgcagtccct ttttccaaat aaacggtgtt caaaagcttg catcttcgta ataagcccag
    13801 ccaaacgaaa aatataagga actattttta tttggctggg cttattactt gatgtaggac
    13861 atctatttta caacctcaga tcatttattg cgcaatactg actaaatgca actttgtgat
    13921 atttttgtgt acggacaact cctcgatcac atcggcataa gtgagccctt ttggaagatc
    13981 aatcgtatag atatttttgt acactcggta gtcatcaacg aattttacgt caaaattcac
    14041 atcttcgatt acgatatttt tctcttcaaa ataatcggta agaaaatctt tggtttcttt
    14101 tctatggata aattggattt ctaacttttt agtcgtaggt acatggatca cgcgtttcac
    14161 tactgtgagt gcaatagcaa tcccgataaa actgatgatc gcaatggaat agtaacccat
    14221 gccgatggcg atccctaatc cagcaattgc ccataaagaa gcggcagtcg taagtcccgt
    14281 caccgattgt ttcgtgacga tgatcgttcc cgcgcctaag aaaccgacac cactcaccac
    14341 ttgagcaatc agccttgctt catctgaacg aatcgttcca gcaagctgtg gatactctaa
    14401 agcatctctt aatgcaccac tagcgatctc aacttggatc aatgcaatga tacatgctcc
    14461 catacaaact aaaatatgcg tacgcatacc tgctggacga tttttatatt gtcgttcaaa
    14521 accgattgct ccgccaaata gcatagcgat ccctaaacgt aaaataatct ctggaattgt
    14581 caataccata tcattcatct aaacaccctc cagtccaata tatcatgtct gaaaggagca
    14641 gacaaaaaaa gtgcaatttt tacctcttac gaaaaaagga ctgtgacaaa atttttgtca
    14701 tagtcctttt tatgaataaa cggtgttcaa tcagctgacc ctcggtatta ctcaacaaac
    14761 ggcaaaatat ggggagctat ttttcctttg tcttctctaa tactcaggtc aaaacgctga
    14821 tttcacaacc tctttagcct actattgaaa attacaactt acttgctgct tcttcatcga
    14881 tgatcacaac aacattgtcg tgattttgca atacacttgc tggcaaatga ttcgttacag
    14941 gtccttcaat catccctttg attgcatcag ctttgctttc gccaaatgcc atcaagacga
    15001 tttctttact tttcataatt gaaccgattc ccattgaaac agcttttgtt ggtacatctt
    15061 ctgctttatc gaagaatcgt ttgtttgcat tgattgttga ttctgttaag tcaactactg
    15121 aagtcgttcc gtcaaatgaa gcaccaggtt cattgaaacc gatatgtcca ttttgcccga
    15181 ttcctaagat ttggatatcg attggatgtg catcgatgat ttgatcatac tttttgcatg
    15241 ctgcttccag atcttctgct tttccatctg gtacaaacgt ttctttgaat ggttttttat
    15301 caaataattg gacatccatg aaatgacggt agctttgttc atctgaaccg cctagtccaa
    15361 cgtactcatc taagtttaca gaagtcattt cactgaaatc caaatcgctt gaagtcattt
    15421 ctttatataa agtaactggt gtgcttcctg ttgccaatcc taatactttt gcacctttgt
    15481 tcataccttc tttgatgatt tcaaatgctt tttttccgcc ttcttcagcg ttttttacac
    15541 gaatgatttc catagtgatt gctcctttat attttggtat agcccaatta tagcattctt
    15601 tttttctttt tacaataatt agtatagacc aaattgcatt tttttcattt tcttgacaat
    15661 aaataggaat ccgtgttagg tttcataaga tggttcaatc aaacaatcca tgttgcatga
    15721 aaaccaagca agtgattcac tgtggacgct ttatgtctgc agttttttta tgaaaaagga
    15781 gttattatga gtatactgac attaaacaac attacccagc aatttggcga caaaatcctc
    15841 tatgaaggtg tcgatctgca attaaatgct ggcgaacatc ttgggctgat tggacaaaac
    15901 ggagccggta aaagtacatt gattaaaatc atcactggtg agatcttacc agatgacgga
    15961 cagatccatt ggcaaaaaaa tattcacaaa ggttacctcg atcaatatgt ggaagtcgat
    16021 gaaacattaa caatcgaaga atttctaaaa actgcgtacg caaaagaata cgaaaaggaa
    16081 gaaaaaattg cttttcttta ccaaacatat agcgagacct tcgatgattc cttgctggaa
    16141 aaagctggga aactccaaac ggaattagac caaggagtgt tttatcaaat cgacacctta
    16201 gttgctgaga tgagcagcgg gttggggatc gatgtcttag gtttagattc actgttgaaa
    16261 aatctaagtg gtggacaacg ttcaaaggtg attttagcaa aactactttt agaaaaccca
    16321 gatgttctga tcttagatga accaaccaat cacttagatg atcagcatat ccaatggctg
    16381 acgcagtttc ttcaggattt tgacggtgcg tatatcgtga tttcccatga ccaagaattt
    16441 cttgatcaga tcaccaccca tatcgcagac atcgagtttg gaaagattac taagtatact
    16501 ggccatctaa aacaagcatt gaaacaaaag gaacaaaatc gcgaaagcta tttgcgccaa
    16561 tatcatgctc aacaaaaaca aatcgaaaag accgaagctt atattcgaaa atacaaagcc
    16621 ggttcccgat caacgatggc aaaaagccga caaaaacaat tagataaaat cgaacggctg
    16681 acgcctccag cttcagcaag taaagctgtc tttgattttc cattctcgcc tattgtcacg
    16741 acattagcag tggaaacaac tgatttagtc atcggatatg atcgaccgct acttgcaccc
    16801 atcaatttga ccatccgctt tggtgaaaat attgccattc gaggcttcaa cgggattgga
    16861 aaatctactt tattaaaaac actgatcggt gagattgaaa aaataagtgg cgatttccac
    16921 ttcccagata acacaaaaat caattacttt tcacaagatc tgtactggga aaatccatta
    16981 gaaacaccac tacaatattt aagcagtcaa tttccaaaag cgactatcaa agaattacgt
    17041 cgccaactag ctaaagcagg gttagttaat caattagcaa gtgaacctct cactacatta
    17101 agtggtgggg aacaaactaa ggtgaaactt gcacagttga caatgaacca aggtaattta
    17161 ctgattctcg atgaaccgac gaaccatatc gatcaagaaa cgaaagaaag cttacaagac
    17221 gggatacaga aatttccagg aacagtaatc atcgtctctc atgaacaaga attttatcaa
    17281 gatcttgtgc aacgagtcat tgagattgaa ccaaaataaa cgtttgattg aatagttgaa
    17341 aaaaaggagc tgtgacaaaa gttttgtcac aactccttct tctgaataaa tggtggccaa
    17401 ttagctgacc ttcggtatta gctcaaaaaa cggcaaaata tgaggagcta tttttatttg
    17461 gctattctta ttacttgatg cagaacagct tttcacaacc tcttttcgtc tattttttat
    17521 tttcgagatt tctctttcct ttaaaaacaa ctgtttgaaa gaagaacgca catccttgaa
    17581 aaacaagccc gctaaccgtt acacctaacc agccatactg ctgccacatt gtcgtcccta
    17641 gcaatgaacc agccgcacct ccaataaagt acataaacat aaatacagta ttatttcggt
    17701 tgcttgctgc ttcacctagc ccttgtactc gtgtttgatt ggcgacttga ccaaattgat
    17761 tcccgacgtc taataaaata atagctaaaa gcaaaatggc caaatggctt ccgccaacca
    17821 ataaagcaat aaagcttatt gtttgcataa caagtcccat atacacgatt tttctttcag
    17881 aataagaatc tgccagtcgt cccacgatag gtgcagcaac tgctccagac aaaccaaaaa
    17941 tcgctaatat ccctgcttcg aatgttcccc aattgtaggc gggactgctg atataaaaaa
    18001 tcaacgtaga ccaaaagatc gaaaatgttc caaacatgaa aaatccactg actgccgctt
    18061 ctcttaatag acgttgagaa gcaactaaat gcggcaaact tttaagtgaa tcaaggtatg
    18121 aaacagctgt actactttgc tggacagaac gcggcatttt ccagtaaagc agaagtgtaa
    18181 gaattccgac tgcacataaa gcaaataaat agatcatccg ccaagaaaaa agactggcaa
    18241 caataccaga taccgtccga gaaagcaaaa tccccacgag taaaccgcta agcaatgtac
    18301 ccatgacctt ccctcttgct tttggtcccg ctagtaccgc accgtaagga atgatgattt
    18361 gcggcacgat cgaaagtaat cctattaaaa aagaagcgca tgcaaataac gtaaacgaag
    18421 gcgcaaaaaa agcggccaac agagagacgg cagataacgc tgccattcga atgatcaatt
    18481 ttggacgatc caccacgtcc cctaaaggaa ctaaaaacag taaccctaac gcatagccta
    18541 attgggtcaa catcgtcaat atcccgactg cacttgtgct aacagataaa ctagctgcta
    18601 cttttgttcc aatcggctgg atgtaataca tattggcaac aacaacgcca caagtgattg
    18661 ataatagtaa aataagcgaa gttgttaaag gttttttttc attactcttc attgatttcc
    18721 cccatttcct atcttagttt acaacttatg gaaaagattg gaaatagaaa aatacaacta
    18781 ttcgccacac ttattatttg tgcttttgtt ttccttgttt tttcttgcga tctttataaa
    18841 tgaagacaac taaccctact agcacagtaa cacttgcaac caacgtgact gcagttttgt
    18901 tatatccgtg aaacagagct tctccaccac acgcatacaa cacagacgaa gggatgactc
    18961 caatcacgat cgacaatatg atttgctgta gaggcaattt catcgcatct gcagcaaaat
    19021 tgaccataga agatggaata accggtatca tatagcctaa cattacacct atttttggat
    19081 gtttcatttg tcggattgct tgcacccaat gattcgtttc ggttttctta tctaaaaatt
    19141 ttaagcgatg cataaggaaa attgcagtga tgttaccaag agcattaccc aaaatgttga
    19201 tgatcgcacc tgtcagtggt ccaaagctca aaccagcaat gactccaact actgaagtag
    19261 gaatacctgg aacagcacac atcaccgctg tcaatacgat caagagtatc gatgcaaaag
    19321 tcccatgcga acgaatatgt gcgagtagct ctgccttact cgctctcgga tttaaaaata
    19381 attggatgtc tgtctgatat tctaaataca accgatagcc cactaccagt atcagaatga
    19441 ttcccaacga gatcaatatc aatcggatta ttttcttttt catatttcct cctgaaaaaa
    19501 atctatattc attaaataat agtttaacat aaaaacaaaa aagcaccgaa atccaacata
    19561 ggatctcagt gcttttaaat agtaaaaatt atttaacagt tacagatgcg ccaacttctt
    19621 caagtttagc tttcaattct tcagcttctt ctttagatac gccttcttta actggtgcag
    19681 gagcgccatc aactacagct ttagcttctt tcaagcctaa accagttgct tcacgaactg
    19741 ctttgataac tttaactttt tggtctccag ctgcagttaa ttctacagtg aattctgttt
    19801 gttcttcagc agcagcagcg ccaccagcag cagctacagc tacaggagca gcagcagata
    19861 cgccaaattc ttcttcaata gctttaacta ggtcgttaag ttctaggata gttgcttctt
    19921 ttaactcagc aacgatgttt tcaatgttca atgccattat aatttcctcc gtttaattgt
    19981 tcttttttat ttaatgcttt atgcggcaat taagctgcat ctccatcttc tttgtctgcc
    20041 actgctttga cagcgtaagc cacgttgcgg actggcgctt gtagtacaga tagcaacata
    20101 gatagaagtc cttcgcgatt tggcaatttt gccaatgctg tgatttcttc caatgaagag
    20161 actttacctt cgatgatacc gcctttgatt tctaatgctt ttgcttcttt agcaaattcg
    20221 tccatgattt tcgctggtgc gactacatct tcgttactga atgcaacagc tgtaggtcca
    20281 gtgaatactt cgtccatgcc ttcaaggcca gcttttttag ctgcacgtga aagaatagaa
    20341 tttttgataa ctttcatttc tacgttagca tcacgaagtt gtttacgtaa acgtgttact
    20401 tcatcaactg ttaatccgcg gtagtcaacg actactactg atgcagctgc tgtaaatttt
    20461 tcgcttactt catcaacgat tgctgctttt tttgcgataa ttgcttcact catttttgag
    20521 tttcacctcc tgaattttga tggaagagtt ttttgcactg aaaaggcaag tcacttttat
    20581 agttactttg ctcaaccagt attattcatc acctattcac ttcgttcata gtgattctac
    20641 tcgctttgct cgaaaggaaa ctcctttcgc tttgctcaac cagtattgtt catcatctct
    20701 tcacttcgtt catagtgatt cacaataaaa aactctatgc caccgcagac atagagggac
    20761 tattgaatat attcaataaa cgtcctcggt aggaaattaa ggcttgatgc cacctactgt
    20821 cttcggtaca agttaattat taacctcaac tagcttagca tgctaaacta gttgagtcaa
    20881 gaattatttt tcagttggat caatcaacat tctaatattt aattagaaag aagcttggtc
    20941 aacatggata ccaggtccaa atgttgttgt gatcgttaca tttttcatgt attgaccttt
    21001 agctgctgat ggtttagctt ttagcaatac atcatggatt gctgcaaagt tttcaactaa
    21061 tttttcatcg tcaaatgaaa cttttccgat tggtacatgg atatttccag ctttgtccac
    21121 acggtaagtt actttaccag ctttcacttc gtttactgct tttgttacgt ccattgtaac
    21181 tgtaccagtt ttagggtttg gcattaagcc ttttggtcct aatactcgtc ctagacgacc
    21241 tacagtagcc atcatatcag gtgttgcaac aactacatca aagtcaaacc atccgttttg
    21301 gattttttga accatgtcgt cgtcacctac gaaatctgca ccagctgctt cagcttcttt
    21361 tgctttgtct cctttagcaa aaactaaaac agtttgtgtt ttaccagttc cgtttggtaa
    21421 tacaacagca ccacggattt gttggtctgc ttttttaggg tctacgttca aacggtatgc
    21481 aacttcaact gttgcatcaa attttgcaat gtttgtttct tttgctaaag ctactgcttc
    21541 ggcaacagga taaactttac ttgaatcaac tttttttaat gcatcttgca tttttttgct
    21601 ctttttagcc attttggttc ctccttgatt gtggttataa cggttgtacc tcccacgtgt
    21661 tctatatctt tcaatataga gccaactgag aagctgctgt cttagggtgt atagattatt
    21721 ctacagtgat ccccatgctt cgtgcagttc cttcaaccat gcgcattgct gcttcaacgt
    21781 tagctgcgtt taggtcttcc atttttaatt cagcgatttc tcttacttga tcgctagaaa
    21841 ctttcgcaac tttatttttg tttggttcgc ctgaaccttt ttcgatttta gctgcttttt
    21901 tcaatagaac agcagctggc ggtgtttttg taacgaatgt gaatgaacgg tcttcgtata
    21961 cagaaataac aaccggaatg atcaaacctg cttgatcagc tgtacgagcg ttgaattctt
    22021 ttgtgaatcc catgatattg atacccgctt gacctagtgc aggacctact ggtggagctg
    22081 gagttgcttt acctgcagga atttgcaatt taacgatttt ttctactttc tttgccacga
    22141 gacatacctc cttaagtccg tgatgtggtt aattggagat gcatatttct cctcccacgt
    22201 tttatctgat ctgaaaaaat ccagacaaat aatgtgcaaa acaatgcaca ctggtacagt
    22261 atagcaaatt tatttgtttt ttcaagattt ttttctgcta tttttacatt ttgcttgaat
    22321 aaacgctaaa tttattctaa aaaaacaaca atttttcgtt ctcatttctt cttttatgtt
    22381 acacttgcgc tagcatggtt caacagatga aagcaaacaa taacacggtt ttttagaccc
    22441 ttgtacaaag attcctacat aacaaaatga ggtggagaaa atgaaagatt taaggtatag
    22501 aagtgtcttt gatattattg gacctgtgat gattggtcca agtagctctc acactgccgg
    22561 agctgctaga atcgggaaaa tcatacgtag tatatttggc gaacagcctg attctgtaga
    22621 tatttatcta tatgaatcat ttgcaaaaac ctatcggggg cacggaacag atattgcatt
    22681 agttggcggc ctgttaggga tggaacccga tgatgaacgt ttagctgatt ctttgaaatt
    22741 ggcatatgaa gccggtatgg aagtcttgtt tgttcctaag agtgaaaaag ccgaacatcc
    22801 aaactctgta aagatgcttc taaaaaaagg aacacgaaag ctttcagtca ctggcatttc
    22861 gatcggcggt ggcaatatcc agatttctga attgaatgga ttcaaaattt ctttaagcat
    22921 gggtactcct accttgatcg ttgtccacca agatgttcca ggtatgattg ctaaagtgac
    22981 tagtatactt tctgagacca atacgaatat cggcactatg acagtgacac gggaatcaaa
    23041 aggtgaaaat gccattatga taattgaagt agatgatcct caagtagaag aaacggtaaa
    23101 aagactcaaa cagctgccaa atatcgacag cgtaaattat ttcgattaag gagctaaaaa
    23161 aatgttttta tcgattgaag aattagtaga tcaagcaaaa gcttatccaa gtgtagcaga
    23221 attgatgatt gcagttgaaa tggaaatgag tggccgttcc cgtagtcaca tcatcgagat
    23281 catggaaaaa aacctctcag taatggaaca atcaatcaac gagggaaaag aaggtgttac
    23341 ctccgtcaca ggaattaccg gtggtgatgc aaaaaaatta gatgattatt taaatgaggc
    23401 agacttttta agtggagaaa caatcttaaa agctgtacgc aatgctgtag ctgttaatga
    23461 agtcaatgca aaaatggggt tgatttgtgc tacaccgact gccggaagcg cgggtgttgt
    23521 tcctggtgtg ctgatggctg tccgagatcg gcttgatttg acacatgaac agcaattgga
    23581 attcttattt accgctggcg ctttcgggtt agtcatcgca aacaacgctt ctatcagtgg
    23641 tgcagaaggg ggatgtcaag cagaagttgg ttctgcaagt gcaatggcag cagcagcttt
    23701 ggtttgtgca aaaaatggga ctcctcatca agcagcacaa gcagtagcca ttacattgaa
    23761 aaatatgatg ggactgattt gcgatcccgt tgcaggctta gttgaagtcc cgtgtgtcaa
    23821 acgtaatgct ttaggctctt ctcaagcttt tatctctgct gatatggcat tagcgggtat
    23881 cgaaagtgtc attccgccgg atgaagtcgt tgctgcaatg taccaagtcg gacgtcaaat
    23941 gccgtcgatt ttcaaagaaa cagcagaagg tggcttagct gttacgccaa ctgctaaacg
    24001 acttgcggaa gagattttaa ataagagggc gtgataaagc tccttaattt cgagtattaa
    24061 gagattagtt atcttcataa caaaaaacga agagaggact aattgccctt ctcttcgttt
    24121 tttattaatc catcaatttc gtatttttta cttcatcgat cgtttttgtt cctgcaagtt
    24181 gcatcgtgat cgacaattct ttgttcaggt gttcaaaaac agatgttact ccttctgccc
    24241 cacctagatt caacccataa atcactggtc ggccaatcgc aactaagtca gcaccacttg
    24301 ccagagcttt gaagacatgc tctcctcgac ggacaccaga gtcgaaaatc actggtacac
    24361 gtttgttgac tacttcggca atttttggca atacttcaaa ggacgcagga cctccatcta
    24421 gttgacgtcc accatgattg gatacccaga taccatctgc ccctgcagag atagcgatta
    24481 ctgcatcttc tggcgattgt attcctttta cgataactgg aagatgggtg atttctttga
    24541 ttgttttgat gtcatctggt gtcaagcctt gttttgcagc tgcataaatt tctgcgattc
    24601 cttttccttc gccattacca cttgcacttt gttcgctata cgcagctaag ttaggcattg
    24661 gtaatggaaa ttggaattga ttgatgacgt cttcttcacg gtaaccaccg agcgttgaat
    24721 ctgccgttaa aatgatagct ttagcccctg cttctactgc tttgttcaaa atgaactcat
    24781 tgaatctgtc gtctttactc atatataatt ggaaaaactg cggtgcgcct ggcgctgcat
    24841 cggctgcgtc tttgattgtc gtatttgcat aagtgctgat ggaaaagatt gatccagcag
    24901 cagctactcc tttagctgta tcagcttccc ctttttcatg tgccaatcct tgggccgctg
    24961 atggcgcttg gataatcggt gtatctaatt caattccgaa aacagatgta tgcaaatcgg
    25021 ctgagtcgat tccgcgtaat atccgtggca tgattttctt tgtattaaaa gaagtcgtat
    25081 tttctttcat cgtccactca tcttcagaac caccgcgaat atagccaaaa gctcctgctt
    25141 ccatgcgctc ttttacacga ccttctagtt aggcaatgtt tacaatatcg attggatgtt
    25201 cttcattact tgtatgataa gtcatagtag atccttcttt catcttcttg attacatata
    25261 aatcatacta aacttacaaa aagtaagcaa acatttactc tcagatactt ttatcgctat
    25321 tctttccaaa taaatcgact tacaagctga tctacttctt ggttttcatg aagcatacta
    25381 tgagaggcat tttcacctgt cactactttt tcctctattt catttccgcg ttgcttcaac
    25441 aacgcataga ccgctaatgc actgtttaac ggtactgttc catcactcaa gtctgtttca
    25501 tctagttgac cagcaatcaa gaaaaatcga gtggttatag gaatattacc gatcatttgc
    25561 tggtaatctt gatagcggtt agaaacgaca gcaggcccct ttgctaactc atctgtcaga
    25621 gactgcgcac tatcatcgac aaaatcatta aaaggcgcgc caattgctgt taatgtctga
    25681 acttgtggca atgacgtatc ctgtccatac gttcctaaat agcgtagtgc actaactcct
    25741 cccatggaat gccccactat gttgacttta tctatgccgt agttttcttt cagatacaaa
    25801 agacaagttt tgatccattc tgcttgattc cactcgttat ttttattatc ttcaaacaat
    25861 acttggacca tcggattgtc tgcttttcca gacaaatctc ctgttgcttg tatttgtcca
    25921 tctgttccta ctgttaaaac caattctttt actgcttgct gatttttttc caaacgccgc
    25981 aacatccctt taaaggaatt ttctgtcccg ctgtagccgt ggaaaaaaag tgttggaata
    26041 gtggtttttt ctatcacttt actggattct gccgtttttt ctttatcaga aacttcagta
    26101 gtatttatct tcgtcgtctg gcaaccactt aacgcaaaca aactgataag gactaaagct
    26161 gctgcagttg cacgtttgca taaatttttg attggttctg tcatacacct cttcctttcc
    26221 atatagaaaa aagaaagact tgaaaggcca atcatatgat cgacatttca agtctcttta
    26281 ttaatcgatt ttatctactt gatcgaaatc aagttctgta ctggtttcac gtccaaacat
    26341 atcgatgttg actttcaatt tttctttttc tccgtcaact tctgttactt gtccttctaa
    26401 tccagcaaat gcgccttcga tgattttcac gacttcgcca acttctactt ctagatcatt
    26461 ttgtcgcagg ctcataccga tagaacgcaa gatatgattg acttcttctg gaagtaatgg
    26521 tgctggtttg cttccggctc cgtgtgaacc aacgaatcct gtcacaccag gtgtgttgcg
    26581 gacaacatac catgaagcgt ctgtcatgat catttcaaca agtacgtatc ctgggaatgt
    26641 tttatgaacg atttcttttt ctttcccatt tttgacttca gtttcttttt cttcaggaac
    26701 aacgacacgg aaaataaagt cggccatttt catactttgc gcacgagatt cgatatttgc
    26761 ttttactttg ttttcatatc ctgaatatgt atgcaagaca taccagtttt tttcaaaaga
    26821 ttccattgaa ggctcctcat ttcattgtaa aataaaaaaa cctcagtaca cccgaagttt
    26881 ttctttcgct tcattatagc actaaaagta cgttgtggct agcgagttat tttaaaatcc
    26941 agccaaataa gctttggatg gctgtatcca ttacgtaaaa catcaatccg aaaataatag
    27001 aagtttcgat gactactaac gtatctttac ggagttgttt tctgcttggc caagttactt
    27061 gtttgatttc atcttttacg ctttttagaa atttcatgag acaacctccc ttgctactgt
    27121 ttatttcgtt tctttatgta atgtatattg attacaatat ttacaaaact tattgatttc
    27181 caaacgttcg ccgctttttc cttcactgac tgattttgtg tagttgcgag acccacaaac
    27241 ggaacaagcg agtgctgctt ttttctttgc catttacttc tgcccccttg ctgagaatca
    27301 gacttatact tcataacagt atcacgtaac tttttacttg tcaatttgtg aaataaacta
    27361 tctattgttt tggataaaat tatgatatga ttaatttgaa attaagctaa aatgagacaa
    27421 ttggaggtat agcgatgcta ttaaaaacac ttgtatataa gaaaaaagat ctgacaaccg
    27481 ttacagagac tagtacatta gaagaagcat taaaaatttt agaaacttcc ggataccgtt
    27541 gtgtgccgat cttagattct tcaggtaata ttttccgtgg aaacatttac aaaatgcata
    27601 tctatcgtca taaagcaaat ggcggtgata tgacgcttcc tgtcactcat cttttgaaaa
    27661 acgctacaaa gttcatttac ctggatactt ctttcttcaa agtattcttt acgatcaaag
    27721 agcttcctta tattgctgtc cttgacagtg aaaatcattt ttatggtatt ttgacacata
    27781 gtacgttgct taatatgctc tctcaatctt ggagcgtaga acaaggaagc tatgtactga
    27841 caattgcttc cacaggaaag caaggagatt tggctgctat tgcaaaaatc atttcaaaac
    27901 acagcagcat tgccagctgt attactcttg atatcgaaaa agacgaatac attcgacgta
    27961 cattgatcac gctaccggct aacactgaag cgaaagtatg tgaaacgatc gtcaaccact
    28021 tagagaaaaa gaattataaa gtggtagaaa ttgaaaattt gcaaaaccaa gaagcataat
    28081 caaaaagcga aaaaagaaaa cagctaatct gctttctttt ttcgcttttt ataattagac
    28141 acgcttttca aaatcaagcg cgcgatctaa ataatacatc aatggtgctg aaacagccat
    28201 gacgataaac tcacttaatg caagcgtgcc ataggttgtc caaaatgctt ggccacctgt
    28261 tggaactaac atataagcaa tcagtcccat gctgatggta aagaagatcg tgttcaatac
    28321 taaacgcatc tttacatttt tcactttgtt ttgcaataaa gctgtcaggc ctaaagaaag
    28381 caatgtttgg ccgccaccaa aaaccacatc catgatacca aaaccaaaga aggcattata
    28441 gacgatgacc ccgcctaaaa ctccccaaag caatttacgg ttaaagacaa ctaaatgatt
    28501 caaactttct gaaatacgaa actggatcgg ccctgaggat acaggcgcaa ctaaaatagt
    28561 taacgctaga taaagagcca tgatgatgcc atttacaacg attacggata accgttgtct
    28621 gcttttcgtt ttttgcattt tctctcctct tttctcctag tttttttaac gcgggatggt
    28681 tgatgaaccg cggttatgtg aaacaaacaa taattataac agacttttca tttatttcaa
    28741 tccgtcttat ctgcttttaa aaaagcaaaa gcttaaacaa tgtaaacgtt tgctaaaaat
    28801 cgtgggatat ggtataaagt aagtgtaagg ggtcataaga attcccagta aagcagacga
    28861 agtaggatct gcaagaacag aattgttgtg aaacttaaat aaataaaaaa ggatgtggga
    28921 atttgagcaa cctcttacta gagaacggtc tagcaagcag ctgaagatct aagtggacat
    28981 gccgaacctt ggaacttctc cttacgtaag taatggatca tgcgcttgct aggctttttt
    29041 gtgtttttcg gtcttcggac ctttttcatt ttcctagact tctgctataa taaccaagaa
    29101 ttaattttgt aacagatttt tttgaaaaaa caagtttctt tcattcaaaa aatagaaaaa
    29161 gcttgctaat tattggttaa ccagcaaaca ggagtattaa aatatcttgt tttattgaat
    29221 agcaggagga aatcatgaaa ttccattatc atatattgat caatccatcg gcaggcagcg
    29281 gaaatggcta taaagtagca gaaagaattt taccggtatt aaaaaataag catattgact
    29341 atacacttca ttatagtgaa tacaaaggac acgatgctga gattgctgaa actttggcaa
    29401 aagaaacatt gattccttgg atagaagaag aacagaaaac aatcgatact ttcccccttt
    29461 tgatcatcgt aggaggtgac ggcaccttac atcaggtact tgatactttc tatcagatgg
    29521 aagtagaatt cccagttgct tatattccag caggttctgg agatgatttt gctcgagggg
    29581 caggccttcc aaaaaatccc aaaaaaggac ttcagctgat tttagcagct caatcaccag
    29641 aaaaagtcca cataatggct tatgaagaaa aaataagtga aaaaaaagga atagctgtta
    29701 ataatttcgg tataggatta gatgcagcta tcgtccatgc gacaaatcat tcttccacaa
    29761 aaaagcgatt gaacaaatac aacttaggat cattttcgta tttattctct attctccgtg
    29821 ctttattcac tcaaaaagga tttccaattc tggtagatgc aggaggaaaa caattaagtt
    29881 tttcaaacgc atttttatgt acagcaacga agcatccttt ttttggcgga ggaatcgcga
    29941 ttgttccaac tgctgatgcg tctaaaccag tcattgattt tgtcgtggtc gaacgaatca
    30001 atttctttaa aatcttgtgg ctcattcttt tacttataca gaaaaaacaa ttgaaatcga
    30061 aatatttcca tcactacaca acaaacagat tacgtatcgt ttcaacaaca ccgcaacatg
    30121 gtcaagaaga tggagaagaa atggaaaaac gaccttttga tatcacaata accacaacta
    30181 aacagttgtt ttggtgttaa taaaagaggt tttgtcagaa gctacatctg gaacaccgtt
    30241 tattcggaaa aaaggactgt gacacgactt ttgtcacagt ccttttttta ctatgcagtt
    30301 aattctttgc ccaattgggt tgccatcgat agtttctttc tttacccaaa ataaattgga
    30361 tcattgaaaa caccgcagca aaagcaacta agacacaacc attgaaccaa aaagattctg
    30421 caaatgtttg attgtaactg agtgtttcac tgatcaagtt attattttga acaaaccaat
    30481 atgttggtgt gaatgccgct attttgctaa ccatttctgg caagtattct gctggtacaa
    30541 acaccccacc gataaagcaa ctgcctgtga taaaaatatc tttgatccca ccgctggttt
    30601 ctgagttttt taccaatgaa gtgatacaag ccgaaaagct aatgatcggc aataaaaaca
    30661 agaaagtatt taaaatatga taccataccg ttatatccca cgaggtgtgg atcactaaca
    30721 gaaccattag aagtaagaag ccactgaaca acagcagcga gtaactaatc aaactaaagg
    30781 ttactttgcg attcaagttt cgtttggtaa tgggcgcact ctggtttcgt ttactgatct
    30841 ctttgcgatt aaatgctaga ttgatgacgc tgaaaccgct aaaaatggat agaaacaacc
    30901 cataagacaa gaaattaaag gttgtgcccg tcaacagctt tttcatccgc tgactatatc
    30961 ccgcacttag ctcaacttct ccagtaaccg agagtgccat ttgtgtctgt tccatagctg
    31021 catcgattgt tttatcttct tcttgaaaca gaatcagtgt ttgcaaaaag gtgttcactt
    31081 gctgtgtaac aactgtttta gaaaaggcat ctgggcgact ctgtatcttg atcgtcggca
    31141 tctttcctac ttcgacttgt tcggtaaagt ctgcaggcag atgtaagata atgtttacac
    31201 gattaaaata aagagcatca tcgatttctt tttgtgaggt tccatccaat gtaacgactg
    31261 tttccttgtc attcaaatat ttacgaaggc catcgactac aggcgactct tcttcactaa
    31321 tcaaggcgat tttcgcatct tgcagagagg tattttcaac atcaatattt tgcgcaaaaa
    31381 agaatgtggc aacaacagtg ataattacac ctatcagtaa attcgattta tttgctttaa
    31441 gcaatgtcca aatcgtttta taaagcgtca taacgcaccc ctctttccat ccaagcattt
    31501 cctaacgcaa aaaggaaaat aaatccgatc aaccatagaa gattggtata aaaactagcc
    31561 aaatcagtgt agaaataaag ttt
//
LOCUS       DARDHA010000015        30674 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000015 DARDHA010000000
VERSION     DARDHA010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.015"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(92..472)
                     /gene="yajC"
                     /locus_tag="SDA10_000593"
     CDS             complement(92..472)
                     /gene="yajC"
                     /locus_tag="SDA10_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEG4239523.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(569..1714)
                     /gene="tgt"
                     /locus_tag="SDA10_000594"
     CDS             complement(569..1714)
                     /gene="tgt"
                     /locus_tag="SDA10_000594"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEG4239524.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1932..2630
                     /locus_tag="SDA10_000595"
     CDS             1932..2630
                     /locus_tag="SDA10_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEG4239525.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2798..5437)
                     /locus_tag="SDA10_000596"
     CDS             complement(2798..5437)
                     /locus_tag="SDA10_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG4239526.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6056..7087)
                     /gene="queA"
                     /locus_tag="SDA10_000597"
     CDS             complement(6056..7087)
                     /gene="queA"
                     /locus_tag="SDA10_000597"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEG4239527.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7280..8455
                     /locus_tag="SDA10_000598"
     CDS             7280..8455
                     /locus_tag="SDA10_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG4239528.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8459..9100
                     /locus_tag="SDA10_000599"
     CDS             8459..9100
                     /locus_tag="SDA10_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4239529.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9097..10014
                     /locus_tag="SDA10_000600"
     CDS             9097..10014
                     /locus_tag="SDA10_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG4239530.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10011..10682
                     /locus_tag="SDA10_000601"
     CDS             10011..10682
                     /locus_tag="SDA10_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4239531.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11223..11711)
                     /locus_tag="SDA10_000602"
     CDS             complement(11223..11711)
                     /locus_tag="SDA10_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HEG4239532.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11912..12178)
                     /locus_tag="SDA10_000603"
     CDS             complement(11912..12178)
                     /locus_tag="SDA10_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG4239533.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12178..12441)
                     /locus_tag="SDA10_000604"
     CDS             complement(12178..12441)
                     /locus_tag="SDA10_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG4239534.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12917..13102
                     /locus_tag="SDA10_000605"
     CDS             12917..13102
                     /locus_tag="SDA10_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG4239535.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13244..15067)
                     /locus_tag="SDA10_000606"
     CDS             complement(13244..15067)
                     /locus_tag="SDA10_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEG4239536.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15665..18370)
                     /locus_tag="SDA10_000607"
     CDS             complement(15665..18370)
                     /locus_tag="SDA10_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEG4239537.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18676..19623)
                     /locus_tag="SDA10_000608"
     CDS             complement(18676..19623)
                     /locus_tag="SDA10_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEG4239538.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19813..20082
                     /gene="rpsN"
                     /locus_tag="SDA10_000609"
     CDS             19813..20082
                     /gene="rpsN"
                     /locus_tag="SDA10_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEG4239539.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20258..20620)
                     /locus_tag="SDA10_000610"
     CDS             complement(20258..20620)
                     /locus_tag="SDA10_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEG4239540.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20631..21752)
                     /locus_tag="SDA10_000611"
     CDS             complement(20631..21752)
                     /locus_tag="SDA10_000611"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEG4239541.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21768..22118)
                     /locus_tag="SDA10_000612"
     CDS             complement(21768..22118)
                     /locus_tag="SDA10_000612"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEG4239542.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22248..23759)
                     /locus_tag="SDA10_000613"
     CDS             complement(22248..23759)
                     /locus_tag="SDA10_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG4239543.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24081..25433)
                     /locus_tag="SDA10_000614"
     CDS             complement(24081..25433)
                     /locus_tag="SDA10_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEG4239544.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25586..26662)
                     /locus_tag="SDA10_000615"
     CDS             complement(25586..26662)
                     /locus_tag="SDA10_000615"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEG4239545.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26858..28603
                     /locus_tag="SDA10_000616"
     CDS             26858..28603
                     /locus_tag="SDA10_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEG4239546.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28883..29320)
                     /locus_tag="SDA10_000617"
     CDS             complement(28883..29320)
                     /locus_tag="SDA10_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEG4239547.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29304..>30674)
                     /locus_tag="SDA10_000618"
     CDS             complement(29304..>30674)
                     /locus_tag="SDA10_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239548.1"
                     /translation="FEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTIL
                     PFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSSV
                     LLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAAS
                     FTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLLF
                     DERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFYV
                     GILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIPN
                     GFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMTL
                     LFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKAK
                     KNEAIH"
BASE COUNT         8714 a         6539 c         5726 g         9695 t
ORIGIN      
        1 atatcccatg ctcttatctc tttctttttt gaataaaaaa tgaggtagga catctttctg
       61 ccctacctcg tctttaaata agctttgata cttatttttc ttccttctct tctgtctctt
      121 ccagcgtttc tgttgtttca gtttttactt caactgtctc agctggttct tctgttgttt
      181 caacagtagt cgttgcatca tttgttacga cagtccctgg tttgaccgtt ttgattgctg
      241 cacgatcgta ctcaaggaag atcccttcgc agtctaaaac aactgtccgt ttgtcattgt
      301 cgatttctga aacaactcca tgtaatccgc cgatcgttac gactgaatca cctggtttca
      361 tgctatctaa caagttttga cgttcttgct gttgtttctt ttgcgaacgt gacataaaaa
      421 accacattgc taatagcgcg ataaacatca caagcattgg taatgttccc atttttttca
      481 cctcactaat tctgtatcta caaaaaacac tttagcagaa aaatgctgat aacactagtt
      541 ttttacttaa tttttaaaag attttccttt agaagctttt tgcgttttct ttattgaacc
      601 cgtattcttc aaaaaaggct tctctaaatt caagcaaatt atcatccatg attgcttgac
      661 gtacttgttt cattagattc aacaagaagt ataaattgtg gtaagaagtc aaacgaatcc
      721 caaatgtttc atcacatttg atcaaatggc ggatataggc acgtgtatag tttttacatg
      781 tataacaatc acatttctca tcaattggac ggaaatcatg ggcatatttc gcatttttca
      841 ctactagacg gccttgagaa gtcatacatg tgccatttcg agcgattcga gtaggcaaga
      901 cacagtcaaa catgtctact ccacggataa caccatcgat caatgaatca gcagcaccga
      961 cacccatcaa gtaacgtggt ttgttttcgg ggatcaacgg tgtagtgaat tctaatacac
     1021 gattcatttc tgatttaggt tccccgacag ataacccgcc gattgagtaa ccagggaaat
     1081 ccatcccgac tagatctttc gcgctttgac gacgaagatc ttcaaaccct gctccttgga
     1141 tgattccaaa taatccttga cggtctggat tagcatgagc tttcaatcct cgttctgccc
     1201 atcgagacgt acgttctact gattttttca cgtagtcgta actttcatcg aatggtggac
     1261 attcgtcaaa actcatcatg atatcagagc ccaatttatt ttggatgttg attgcttttt
     1321 ctggtgataa aaacatacgc gaaccgttca agtgattttt gaaatgcacg ccttcttctt
     1381 cgatattccg catatcactt aatgaaaaaa cttgaaagcc gcctgaatct gttaaaatcg
     1441 gttgatccca gttcataaat ttatgcagtc cgccagcttc ttctactaag tcttcaccag
     1501 ggcgtagcca taaatgatat gtattactta aaataacgcc agcacccatt tctttcaact
     1561 cttctgggga cattgttttt actgtagcta atgttcctac tggcatgaac attggtgtgg
     1621 ggaaagttcc gtgtggcgtg atcaattccc ctaaacgcgc gccagtatgt ttttctttct
     1681 tgattaaacg atagcgaatg gcaggttgcg tcattcattt ccctactttc tatataaaat
     1741 atcttgacaa ctcccaccat cataatcgtt tcaagtgctt tttgcaagtt ccctcggctt
     1801 ccttctactt tatttaatat tctttttttt atcctttttt tatgaataac aaaaaaaaga
     1861 aaaacattat gacagaaata ttttttgata catggtatat tggtgttatt aaagaaaagg
     1921 agggaaaaat catgcaatct tctgcagaat taaaacgcca agcaaaagag agtctaaaag
     1981 gacgttgggg acaagctgtt ttactaaatc taatccctac attgattggt attgctacta
     2041 ttcttcttat tatgattcct attatcttgt ttgccaccac catatatgat ggatcagcga
     2101 gcgtctcaga agcgacaact tcaggaggcg gtagttcagg aggcggtggc ggaattattt
     2161 ccagcatcat tagcgctttg ttcatgagcg gtatttcttg gacttatctt gatattttac
     2221 gtggacaaaa agatcagatc gaaccattca aagatgcatt tcgtggattc aaaggtttgt
     2281 ttatcggcgg agtgatcagc ttagctgtgc tgatctcgat ctttacaaca ttatggggtt
     2341 tgctattagt tattcctgga atcattaaaa gctattctta ttctcaaagc tattttattt
     2401 actacgatat cgtccaagaa acaggacaaa cacctaaagt actggatacg atcactgcaa
     2461 gtcgaaaatt gatggatggt tataaaggac aacttttctg gttagatctt tcatttatcg
     2521 gctggcatat cttagcatta gcaactgctg gtattggtta tctatggctc agcccataca
     2581 tttctgcaac aaaagcagca ttttatgaac aattgccaaa agatatttaa aagagagtgt
     2641 gacttgtgac cgagggggtg gcaggagaac accgtttatc gagaggcagt aaaagaaaca
     2701 ttcagcccat tagcaaaaag gaggtgtgac aacattcatg tcacacctcc tttttgtata
     2761 gttccagtaa aaagaaaatc aacttagaaa cagttaatca ttcctcttct tctgcttccg
     2821 gtaatcgatg gaacttgatc agttcattca ccgcgattgg tacgatagat aaaatcccta
     2881 ccaagaacca gtgattcaaa ctgatgtgta ctaggccgaa tagttcttgc gtaaatggca
     2941 gcaaagcaac agccgttgta atagctaatg ccagtaacgc catttctacc aaagctttat
     3001 tggtcgctaa attgactttg aagatcgatt ggctgctgcg tacattgaac acatgcagaa
     3061 tcgatgaata agctaagatc aagaatgcaa cagtttgacc gacatgctga cttgcgtcta
     3121 cccatgggct gatagtatcg acataactac ctaaataata accaaacaag gttacgatgg
     3181 tgaagacccc tgcatttatg gcaatttttt gcaataaacc tcgagcaaag atcccttcat
     3241 ttttcggaat gggtggctga tccatgatgc cgtgttccgc tggctctctt cctaaagcaa
     3301 atcctggaag tccatcagca actacattga caaacagtag ttgcacagct gtaaatggtg
     3361 ctccccatcc taacagcatc gcaatcaaaa caataaagat ctccgaaata ttacagctta
     3421 gcaaaaagtt gatagcttta cgaatattct gataaaccga acgtccttga ctgacagcgt
     3481 ctacgatcgt cgcaaagttg tcatccgtca aaatcatatc tgcggcactt tgcgccacat
     3541 ctgttcctgt gatccccatg gcacacccaa cgttacttgc tttcaacgct ggtgcatcat
     3601 tcacaccatc cccagtcatc gctacgactg cacccgtacg ttgccaagat ttgacgatcc
     3661 ggatcttgtc ttctggcgtt acccgagcat agacagagag agctttgaca cgcgcatcta
     3721 attcttcgtc tgataattga tgcagttctg atcctgaaag tgcttctgac ggatcattca
     3781 agatacctaa ttctttcgct atggcactgg cagtcaccac atgatctcca gtgatcatga
     3841 ctgttttgat gccagccttt ttcgctcgct tgattgctcc tttactttct ggacgtggtg
     3901 gatcgatcat tccgatcaga ccaatcaaac gtaagtcttt ttccagtgct tcagaagaaa
     3961 tatcttgcgg ttcttcgtca taagtagcat aaccaacagc aatcactctc agtgcccgtt
     4021 taccaaagcg atcattaacg atcgccgctt gttctacatc gcctgaacta aagcgcggag
     4081 caagaacatc gaatgctcct ttggtgattg agatgtattt ttcgcccatt tggtgaactg
     4141 ttgtcatcat ttttcgttca gaatcaaaag gaagctcgtt tactcgtgga tatttttctt
     4201 ctaattctgc ctttgtatga taattctctt caacagccct tacgatggct acttctgttg
     4261 gattaccttt gataacaagg tcgtcatctt cttgctcgac cgtcacatct gtacatagtg
     4321 cagccatttt caatatttcc atcgcgctgt tcgtcattga atcttcgata ttcgttactt
     4381 catcttcttt gctccatacc cggcggacgc gcatttcatt ttgtgtcagc gttcctgttt
     4441 tgtctgagca gataacatta gcggttccta gcgtttcaac tgctggcaac tgtcggatga
     4501 tcgcatgttt cctcgccatc ttctgcacac cgtatgcaag tgtcaacgta acaataacag
     4561 tcaaggtttc cggtacagct gcaacagcca aggaaatcga agtcatgaac atctcaagga
     4621 gtggttctcc ttgcagttca ccaatcacaa atactaaagc cgcagccgct aaagcgatca
     4681 tactgattcg tttgcctagc tgattcaatc ggacctgtaa aggtgttttt tgcatagtat
     4741 tttcgttcaa aagatccgcg atcttaccca tttctgtagc catgccaatg gcagtgacga
     4801 tcgctttccc acgacctgca gcaaccgtac atcctttaaa taccatatta agttggtcac
     4861 ctaatgactc atcttcttca gcaataaatg ctgcatcttt ttcaactgct tcactttctc
     4921 cagtcaacgc cgattcttct acacgtaatt gtgatgcttc gatcaaacgg gcatcggcag
     4981 aaatagcatc accggactct aaaacaagaa tatcacccac taccagttgc tctgactcaa
     5041 cagtggtcac tttgccgtct cgtatgacag ttgccgtttg tttattcata tcttgcaagg
     5101 attccagtgc tttttctgcg tttccttctt ggacaatcgc caataccgag ttgatcacaa
     5161 cgatagcgat gatcagcagt ccttcaaata aatccccatg ttctgtagca aatgcggtat
     5221 aaaaggaaat caccgctgct acaagaagga tgatcgttgt aaaatcggac aagcttcgaa
     5281 agaatttttt gatcatcgat tctttcggcg cctcatcgaa tttattcaat ccctgctttt
     5341 ttcgccgttc atccacttct tgtgaagaca acccagtatc tggatctgct tctgttacct
     5401 tctttacttc ttctattgtc tgcttgtatt cttccatcct attcctcctt taaaatcttc
     5461 gtacaaaaaa aggtacttat tttactataa catccttttt ttatttactt caaataaagg
     5521 gcttatcgat tatttgttaa gaaagcgtaa aatttaaagt gcttatattt gcaaacgcta
     5581 ccaaaggatg ctattatata gatgtaagga aggaggatac catcaaatag acagaagaat
     5641 ttctcgatag aacagtgtaa gcaaacaaag ttgttagaag tacaggtttc acccagacat
     5701 gcgtgtagag tacaaaccta taaatagtga atgcgtatgg atgtttggat cacgaagtat
     5761 ccgaaacaag aagtaatgcg atacgtaaca attgaataac gtaaacgtct gaaaattgaa
     5821 taggaacaag agatgtttca aatgttgaag atgtaacgaa aacaaaattt ttgtttaaca
     5881 tgaagaaatt caacaccgtg ctttgcatgt atgatggaaa gttacaatca tttctgttta
     5941 tttgaatcct tccgaggtac aagaaagccg agcttatcga gagctcggct ttttctttgt
     6001 cataaaatct ttgtaaagct tttttgttat tcaaaactag cggcttttca taaccttatt
     6061 tcacgaacat cgcatctcca aaactaaaga aacgatagcg ctcttcaatc gcatgctggt
     6121 aagcagataa agtcaattct cttcctgcaa aggcactaac taacatgact aaagtcgatt
     6181 ttggcaaatg gaaattagtt gagaaagctt ctaccacttt gaattcatag ccaggcgtaa
     6241 tgaaaatatc agtccatccg ctgtctgctt tgatttctcc attaaatttc gttccaatcg
     6301 tttctaatgt tcgaattgaa gttgttccga cagccacgat tttcccgcca tttttacgta
     6361 cttggttcaa tcgctcagcc gcttcttccg tcaaacggta gaattcacta tgcatatggt
     6421 gttcttcaat tttatctacg ctgacaggtc ggaatgtacc taaccctaca tgtagtgtca
     6481 gatagactaa ttcaacaccc tttgcttcga tctcatcaag taattctttc gtaaaatgca
     6541 atccagctgt tggcgctgca gcagaaccat tttctttcgc atagacagtt tgatagcgat
     6601 ctgggtcttc cagacgttct ttgatatatg gaggcaatgg catttctccc aatgattcaa
     6661 gaatctctag aaaaattcct tcatactcaa agtcaatgat ccgtccgccg tgttccaatt
     6721 cttctttcac tactgcttgc aatctgccat caccaaaaga gattcttgtc ccaactttgg
     6781 cacgtttcgc aggtttgatc aatgtctccc acgtatctcc ttgtgtattc gttagaagaa
     6841 ggacttctaa atgcgcacca gtatctggtt tttctccata taaacgagca ggcagaactc
     6901 gtgtattatt cataactagc gcatcaccag gctgtaactc ttcaatgatt tcatggaaat
     6961 gtttatcttc catctctcca gttttccgat ccaaaacaag taaacgagaa tgatcgcggt
     7021 cttttaatgg tgtttgtgcg attaattctt ccggcaagtc aaaatcaaaa tcttctgttg
     7081 ttaacatatc gtcactcact ttctatcatg tgcttcatca ttctatcatg tttttcgttc
     7141 tgttggcaaa atcagcactt tttcacttct attttttgtt ttcctaaata aaaaaactac
     7201 cctatttgta ttttttcatt gttttaggca tactagtaat agatagttct tcattgaaaa
     7261 tagaaaaaga ggtggcacca tgattgaatt tcaaaatgtg tcaaaaattt ataaaggtgg
     7321 aaaagtcgct gtagaggacg tcaatctttc atttgaaaag ggtgagttca tttgttttat
     7381 cggtacgagc ggaagcggaa aaacgacgac gatgcggatg atcaaccgaa tgactgatcc
     7441 aacacaaggg aaaattttga ttgatggaaa atccatccaa gaaattgatc cagtggaatt
     7501 gcgacgtcag atcggctatg ttatccaaag tattggattg atgcctcaca tgacgatccg
     7561 tgaaaatatc actcttgtcc aaaaattgtt gaaagtcagt caagaagaac ggaataaaac
     7621 agctgaaaaa atgattgatc ttgtggaatt gccccgtgag atgttagatc gctacccaca
     7681 tgaattatct ggtggtcagc agcagcgtat tggagtcgtt cgtgcccttg cagcgaatca
     7741 agatatcatc ttgatggacg agccattcgg tgcgcttgat ccaatcactc gtgattcctt
     7801 gcaagacctt gtcaaagatt tacaagagcg tttgggaaaa acgatcgtat ttgtaaccca
     7861 tgacatggat gaagcgatta aactagcaag ccgtatcgcc atcatgagtg aaggccgtgt
     7921 gatccaattt gatacaccag aaaatatttt acgtcatcct gcaaacgatt tcgtagaaga
     7981 gttgattgga gaagatcgcc tactacaagc caagccaaat gcgacaagag tcggcgaagt
     8041 tatgctaaat acagcaatca cgattacacc ggaaaaatca ttacaagaag caatcaaact
     8101 gatgagggaa aaacgtgtag atacgttgtt agttaccgat aattcgaatg tattaaaagg
     8161 atttatcgat gtagaaacgt tgaataaaaa acgaggaaaa gtatccagtg tcggcgacat
     8221 tttgaataaa catgtttttt acgtaaaaga atccgcctat cttcgtgata ccttgcaacg
     8281 cattttaaaa cgcgggctga aatacgtgcc ggtcgttgat gaacagaaca aagtcgtagg
     8341 tatcctgact cgtgcatcat tagtggatat cgtctacgat gtgatttggg gagaagatcc
     8401 cgcaactact gatgttgcag agtcagctac tcctgaagaa tctaaggagg tctgacctat
     8461 gcagcagttt tttcatcagc acggttcaga aattctctca aaaggttttg aacatcttta
     8521 tatttcagga cttgctttga tcattggtat catcattgct gtaccgcttg gtgtcttact
     8581 gacacgtttt ccaaaagctg ccacgattgt gattggctta accagtgcct tgcagacaat
     8641 cccttctctt gctttattgg cattgatgat cccgctattt ggcgtaggta aattacctgc
     8701 gattatcgca ctctttattt attcattatt gccgatttta cgtaatacgt acattgggat
     8761 gaaaaatgtc gattggaatt atcgggatgt ggcaaaaggc atggggatga ctgagttcca
     8821 atccattttt tccgttgaat tgcccattgc tatgccaacg atcatggctg gtattcgttt
     8881 agctgccgtt tacgtgattg catgggctac acttgcctct tatatcggcg ctggtggttt
     8941 aggcgatttg atttttagtg gactgaataa ttaccaacca gatctcattt tggcaggtac
     9001 gatccctgta acaattttag cactattggc tgattggctt ctaggtctct tggaacaccg
     9061 gctgacaccg atcgcactta gagaggagaa tgaagaatga aacgtatact aaaaatgacc
     9121 atggttcttt tctctcttct tatacttgcg agttgttcct tccctggtct tgccagcaat
     9181 acagatgaag acaccatttc gatcacaggt ggaatcactt cagaagcaca gatcttagca
     9241 agtctagttg ccggaatggt tgaacattat acagataaaa ataccgcaat catcaacaat
     9301 ctggctacta caactatcaa ccatcaagcg atgatgaacg gagatgcttc aatttcagcg
     9361 gctcgctaca caggaacaga tttaacgact acattgaacc ttcctcctga aaaagatccg
     9421 aaaaaagcgt ttgctaccgt gaaagatgaa tttgagaaaa gatacggtca aacgtggttc
     9481 ccgtcctata gttttgaaaa tacgtatgtc tttcttgttc gaaaagacac cgcacaaaaa
     9541 taccatctat ctaaagtcag cgatctgaaa aatgtagcgg atgaattagt agctggtgta
     9601 gatacttctt ggatcaatcg taaaggagac ggttatgacg gcttccaaga aacttatggc
     9661 ttctccttca attcgatatt acctatgcag attggacttg tttatgacgc agtcgaagca
     9721 ggaaaaatgg atattgttct tggctactct acggatggac ggatcgccag ttatgatcta
     9781 gtcatgttgg aagatgatcg tcaattcttc ccaccatatg atgctgctcc agttgtagac
     9841 aacaagatat tagctgatac gcctcattta aaagaagcct taaataagtt aggtcatacc
     9901 atcagtacgg aaaaaatgca ggaattgaac tacgaagcag ataataatct agtggaacca
     9961 tcagtagtag cacaacgttt cttacaagaa aaccactatt ttgaagggaa gtgaagcaat
    10021 tggataaaat gaatgtcttt caacaatttt tctattattt ccaggaaaat ggctcctata
    10081 tctttgcaca atttatccgt cacttcctga tttctatcta tggtgtcctc tttgcagcag
    10141 ttgtcgggat tccggttggg atcatgattt ctaggagacg taaattagca aactgggtga
    10201 ttcgtttggc gaatatcatc cagacgatcc cttctctagc catgatctcc attttaatca
    10261 ttggattagg cctgggagtc aatgtcgtga ttgttactgt atttttgtat tccctcttac
    10321 cgatcatcaa gaatacgtat accgggatga tccaagtaga caaaaacatc cttgatgtcg
    10381 gcaaaggcat ggggatgact gctagacaac gactattcat ggtagagtta ccactctctg
    10441 tttctgttat catggcaggc ataagaaacg cgttagttgt tgccatcggg ataaccgcga
    10501 tcggtgcatt cgtcggtgct ggtggtcttg gagatatcat tatccgagga accaatgcaa
    10561 cagagggcac atcgattatt ttagcaggtg cattgccaac tgcattaatg gcgatcatca
    10621 cggactggct tttaggtatt ttggaaagac ggctagatcc agccagccgt acatcacgct
    10681 gatgttattg taaaaaacaa aagcttctgc gaccatttgt tggtcgcaaa ggctttttta
    10741 gttctgtcat ttttaaggca ataatccact ctgattcttc cctccttatg ggtaaagagt
    10801 tgcttttctt agctatttat ccattccgta tactccaact tcatactgcc aacgggttca
    10861 tatctgcatc caccgactaa gtttttttct tatccgctaa ggcattttgc caatccattt
    10921 tcccatcact cgtcatggaa ttcataccac atgatctcta aatactcgtc tgcttgttct
    10981 ttgaattggg catcaattct tacatccttt ggcgtggctt tcatttaagc cttttccatc
    11041 agtcctcgat tgttcgatgc cttcagctgc atcatcaaaa ctggtgactg ccgtatcggt
    11101 aacttcgatc atcttttata caatcgggaa cttgccatcc tcatccttgg ctaaaaacat
    11161 atctagccat tccagcttct cgccatggga ggtttgcggt tcctaaaacc ggtacacgat
    11221 tcttatggac tgcatccgtc gcaactagac ttggcgatac gatgatcctt caccagaaga
    11281 gcacccccgt aaaccaattg atcgatatat tgccaataag aaaagacatt ggcgtaaaag
    11341 atatcgatcc ctcgtgatgc attgccgttg atactgctat tcatggccga aagcgccacg
    11401 actttaactt cttccgtttg agtgacattc atgttcggca atgtcgcttt ggatacccgc
    11461 tctgctaaag gaacgacact ggtattgtat ttcgtatctg tatcttttcc aaaagaccat
    11521 gccaacgaat cctctgggaa ccaataagac gactccggtt gattgtccat tgcttttttc
    11581 aacatacttt tggctgtttc tcgataaacg acatttccaa ttgtcttcgc ctcactttgg
    11641 cgataatcga tggtcgttgc cactgccaac actaacaacg atgctccaat tttactgatt
    11701 ttccttgtca cacttcttcc tccttgtttt acataaaccc gtcaacattc cattcctaaa
    11761 ctcaaacaat gacccttcaa aggtggagat ataggaacaa attaaagaaa tcatgcaata
    11821 aatccaagaa ggttggtttt agaacaaaaa aagagtctcc tttttgggga gactctttgt
    11881 taactatcta aaaaggttgg ctcttctcac ttcactcata atgtgtccag gcagaataaa
    11941 tttctacaac tttattttcc tcatcaactt tgtaaaccac tcgatgctgt ctattcagcc
    12001 tgcgtgagta tctgccttca tgtgtaggtc tcagcttttc aaaagactgc gtgggcatat
    12061 atggatcttc cttcaatgtc tgaatcactt cttcaaattg tttctttaaa ttcgtttgct
    12121 tgatttttct taaatccact ttcgcagagt ttttgattgc gactgtatag ttactcatca
    12181 aagagcttcc caatcaatat catcgatatt cgtaaagcta ctgtcgtctt tttcgcggtc
    12241 tcgtacttta tctaaagtac ctgtttgctc aagaaaaagg gtttcttgaa tcgctctcca
    12301 gtcttccagc ccaatcaata cagcattgtt tccactacga tcactaataa tctcgatttc
    12361 agtatgattt tcatttacct ctttcaataa ctgataaaaa tttttacgag cagtgctagg
    12421 attcatcgtt gtattagcca ttgtctctcc tcctttttct gatataatta tagcgtactt
    12481 gattaagtac gtcaaaaaaa tacaataaag cacttaaatt cagcaaactt ttcctctttt
    12541 ttacatagag aaaaagaccc ctttttaggg aggatctttg tccacgctca cttcttactc
    12601 ctttacgact agcggtagcc aaacgtaaag taattggtaa caacgtatca tctttagcaa
    12661 aggtcgaatc caaagcctgt gagtctatgc aacaacacca atacacgaag gttcctcgtt
    12721 gcagtgtgac ttgattgata tcttgaataa cctgaggata cgcacgaaag atttgtaacg
    12781 gttattcaaa actgattttg gccgtcaagt cttctactgc ctcaaaaatg attgcctctt
    12841 tcgtctcctg tcattcatgt tcactatcga tttgatactt gcttagaccg ttgggtttgt
    12901 gaatcaccaa ttaaaaatgg cactgagtta tggtaacaaa aaatggtcac ttgacgaggc
    12961 tgagattcct acttatgaag acacatggca cttaagagtc gaggtaaagg aagaagctga
    13021 aaatatagga ggctgtacat tatttggcaa aggattcgga aattatgatc aagacatcaa
    13081 aatcaaaatg ttttacagct aaataggaag aataataaca tttcatacgc tgttcccatc
    13141 tttaatggct tatagagggc acctttagct gagaataaga caaaacagac cctctagctt
    13201 caattaagag ctagagggtc tgtttttgat tttaaaactt ggtttatttc ttcaaatggt
    13261 agctataggt gctgacaacg atattttcac gccaagacaa tcgcaatctt aaccgcaaac
    13321 ttgtttggtt atggagaata ttctgccagc gtctgtttgg tacaaattga ggaatcaaca
    13381 cagtagttgt atagttttgt ttcgctgcat ttttacttac gatatctaca taacggataa
    13441 ttggattttc aatcgagcga taagaagagt ggacgattga aaaacggaca tctgggaaat
    13501 gctttttaaa ttctgcttcg atttcttttt ctttttcgac atcttcgtct agcgaaacat
    13561 gcatcgcaac gacataatca ccgatcgaac gtgcgtaatt caacgcaccg atatttaccc
    13621 gtgtcacatt cccgactaat acaagaacgg tattcccgtc ataatcatgt agttttgcat
    13681 cattttctaa acgaagctgt tctgctactt ttttataatg atcatggatc ttataaaaaa
    13741 tgaacataac gattggcata atgatgaaga aaggccaaat gtcccctaac cgatagacaa
    13801 acaaaatcgc aatgattgca tatgagatga acgcacccgt aatattggca atcgatttgc
    13861 ttaaccatct ttttccttct tttttccatt tgacgaccat ccctgtttga gataaagcaa
    13921 atggaatgaa cacgccgata gagtataacg gaatcaagcg ttctgttgag ccatggaaaa
    13981 taaacagcaa tacgatcgaa cctagcgcta gtgtgataat cccattggaa tagcctagtc
    14041 gatccccacg atcttgatac atgtgaggca tgaatttatc ttttgccaga ttgtatgcaa
    14101 gaactggaaa agcagaaaaa ccggtgttag cagcaactgc taaaatcagc gctgtagcaa
    14161 attgaaggac gtaatacaaa ataccatgtc caaagacagc tttaccaatt tgagacaaaa
    14221 cagtgacttc tttttcagga acgatcccat accaatagtt gataaatgtg atccccacga
    14281 agaagaaacc taagatcaat gccatcatcg tcaatgtagc agcggcattt tttgctcttg
    14341 gcttcttaaa gaatggtaca gcattactaa tcgcctccac cccagttaaa gaggaagaac
    14401 cagaagaaaa tgctcgtaaa atcaaagcga tagaaacacc cggcacagcc gctcctggaa
    14461 gtgcagtagc ttgcaatggc acagcacctg tcacgatatt atataaacca acaacgatca
    14521 gtaacgagat cacaaggata aatgtgtaca ccggaaataa taggaacgat gcagattccc
    14581 tcagcccacg aagattcagc atcatcagta gcaaaacgat tgtcacagaa atagcgactt
    14641 gatgaccgta gagtgctggc accgctgatg tgatcgcttc agcacctgct gagacagata
    14701 ctgctactgt caacatgtag tcgatcaata aagagccacc ggaaatcaag ccagcattct
    14761 ttccaagatt ctcactgctg acgacatatg ctcccccgcc atgaggataa gcatggatga
    14821 tttgacgata tgatagcgtt aatgaaatca ataaaataat aacaaaagcg gcaatgggta
    14881 gcgaatacca gatggctgca gcagacaaag cgactaaaac gacaacgatt tgttccgtac
    14941 catatgcaat agatgataac gcatcagacg acaataatgc caaagcagca aatcgggtta
    15001 atttgtgctc atcattttca gcagatttta aaggcttacc tactaacaac ctttttaagt
    15061 aatccaattt ctaaactcct ttacacacaa ttttacttca agttttatag tcgaaaattt
    15121 atagtaaaca agatgttata gtacgctcac tgaccttaaa agtcaataaa ctttaagaaa
    15181 ataattggaa aaaaacagaa aaatatcaag cgctttttca aacgctttaa tacagcaaaa
    15241 atctaacatt tttttatggg ctttttcttt gaattctata ataaaaggat agaacttgac
    15301 ttagaaaatc aagttctatc ctttttgata tcttaacttt tggaagagag atgtaaggct
    15361 gctttctacc catcattctt actactcgaa gcatagcgca gttgtcacaa cctcttgata
    15421 aacggtgttt aaaagcttgc atctgcggta ataagcccaa ccaaattaaa aacataagaa
    15481 gctgttttta tttggtcgtt cttattactt gatgcagagc agctttttta caacctcttg
    15541 gtacacggtg ttcaatcagc tgaccctcgg tattagctca aaaaaacggc aaaatatgag
    15601 gaactatttt tccatttttt tctctaatac tcaggtcaaa acactgattt cacaacctct
    15661 ttttttaatg aaaaatatgg ctctcatgcg ccgcttcttt gaatttcgtc attttttctt
    15721 cgatgaacgg atagattgcc gttcctacga ttccaaagac aacaaacaaa ccgatcatga
    15781 tccagattgc tttcgtagca tttggccaat aaattcctcc tgcagattcc cgtaacaagt
    15841 ttaccgcatg agtaaatggc aagaacggat tgatcatttg gaagaatttc cctgagactt
    15901 ggatcggata gttcccgcct ccgccagaga tcgacagtac taagatgata atagcgatcc
    15961 ctttaccgac attatcgaat aatgcagcca agacatagac gatcatcatg aacgctaagg
    16021 cgatcaagac agcaaatgac acgctgtaga ccggattttg gacatctact cctagtaaga
    16081 aaatattccc taatgtaacg atcaagcttt gcgcaatcgc cattgtcaaa aatgtcagca
    16141 tacgagcaac gaattgttct cgtttcgtaa aattgtttcg gtctttcttt cctaaatagt
    16201 attctgtcgt agcaacactt gaaagaagaa cagcgccgac ccacagacat agtgcagtat
    16261 aaaacggggt gctagctgaa ccgttatttg cgattggata aagcgcattg gtttgtagct
    16321 caacaggttg tgtaaagaag tcactttctt ttgttgcgtc tagtttcaat aatttgatga
    16381 cttcgcctag atcgacttct tgttcgcctt ttcggatcgc gtctgctgct ttttggatac
    16441 cagctttgat atttggccag tcattgttga tcaagtccgc agcagcgttc acagctgttt
    16501 cgacttctgg cattttttca tttactgttg tcaatgtacc ggtgatttca ttttttagtg
    16561 ctggccaatc attttggata aaatcagcgg ccaatcctaa tttttgctga atgatcggta
    16621 attcattgtt gtataggtct gccccttcat tgatgccatt gacgatggta tccatatttc
    16681 cattcagcat tgtattggca tcatggattt cttgtccaat tgcaggcatt tctttttgat
    16741 atttttctaa aattgccact gcattcgtta cagttgcttt cgttgagctg agaagcgaat
    16801 caaagtcgat ctggtccgct ttgctcagtg cgccttgtgc agtcccaatc gtatcgatga
    16861 cttggtctag tagagaagtg acagtggaac tgatttgacc tgtattgata ccagcaatga
    16921 tgccattgat attattaaca acggcatcaa tttgatctag caattcatca atagccttcg
    16981 tgtcttcatt ttgaacagct gtgttcaaag cagataggcg gttttgcaga ttgcttagca
    17041 agggttttaa ttgatttagc tgatcaataa ttggctgtaa tttcgtattt ccagcacttg
    17101 tttgcaattt agtcaggaac tcgaccagtt tatcgattgc attttgttgt tgagatattc
    17161 cgtcaataaa gccgttcaat acattgctga tgtttttctt ttcatcatca gtcagcttac
    17221 catcttcaat agctgttctt acagtatcta ttaacgaacc tgtcgttgtt gccacttgtc
    17281 caattgactt caatgtaaca tctacactgt ttgtgatgct tggtatcgca tctttcagct
    17341 gagaggcacc tgattttgtt tggtttgcta gatcacttgc ctgattttct aaatttttca
    17401 catctggcaa gatatcttgt acttgttgga tgatcgtcaa gccttgtttt gcttcatcga
    17461 ttccttggtt cattgtctct tggatcgaag caaaatcgcc gtcgatctct gctagttgct
    17521 taccggcatt ttggatctct gggatttttt cttgcaaatc aaggatgatt tttgcttgat
    17581 ctttcaactc aggcattttg tcattcaatg caacgacttt ttcacccatc tcatctactt
    17641 tagggatgta atccacgaat tcatttgctt tattcagttt ttccttgatt tcaggtagtt
    17701 gactttgtaa ttcgaggact tgttttgtgt agccgtcgat cgtgtccaaa ttttcatctg
    17761 ttgataaaat catatcttta actttattga tacttactag gttcgtatcg atgtcgtaac
    17821 ctatctcatt gaatactttc aataaagtcg aactggctgt tttgataaat tcatttgtga
    17881 tttgcgattg gatcgaactt gctcctttat cggtgatttt cggtgcaatc gcattgattt
    17941 tttcatttac cgtatattcg atttttggtt tcttgatatc accactagta aaactcagca
    18001 gatcttctga gaaatccttt ggcaagtaaa tacctgcgta atactttccg gaacggactc
    18061 cgtcttctaa ttgttctttt gaatcaacga attgccatcc taattgttta tttttatgaa
    18121 gcgattcgat gacttgctcc ccaatggcca cttctttacc ttgaaactct gctggcttgt
    18181 ctgcactgta aacagcaatt ggcagctctc cagtatttcc atatggatcc cataatgctt
    18241 tgatgttaaa ccacgcatat agcgaaggga tgatcatgat cgcgatgatc agaagtgttg
    18301 caatcgggtt tttgaaaatc cgtttccagt caagcaaata taatcgaagc gtatttttga
    18361 ttgactgcat agttctcccc cttcttcttt tttcagctga attactttac catgtatttc
    18421 atactttttt caatcggctt ttttcaaaaa agtcgaattt ttggacatat cacggcaatt
    18481 gtctcattgt aatctcttac atattgagaa ataattaagg attacagcct ttactttatc
    18541 atgaaataaa agggaaagat aattttatgc caaagttttt atctattata cgtatacgca
    18601 aaaaagctaa aacatttttt gtctttgccc ctacaaaaat gtttcagctt ttttcgtttt
    18661 ttattcctgt atttcttatt tttcgaatga caggatgatt ttcccagcag gaggatgtcc
    18721 ttcaactagc tgatgtgctt ggatgacatg cttaagtgaa gcagacagat tcattgaaat
    18781 aaaaacatga aaggctcctt tttggtaggc tttgattatg ccagcaaagg cttctgcatc
    18841 tagatattcc tttcttggaa caaacgattc ataaaagcct tccttttttt gccgcaaatc
    18901 tagatctggg aggtcattta atgcaacata cctgccgcct ggtttcatga tctggatgcc
    18961 ggtttctcct ttgatacttc ctttcgtcgc atcgattaca aggtctgcct gatcagcaaa
    19021 ttgcagacct ggatttgttt tatcataagc agcaaatgct gaagctccta atttttttac
    19081 cttttcctca tttttactgg atgcactggt taagattcgg attccctttt catggagaag
    19141 ctggatcaga cttgacccga ccgcacctga tgctcctagt atcattacag tatctgtcgg
    19201 ctggatttcc gtcaaatggt tgattagatt ataagccgta attcctggcg tgaccattcc
    19261 ggctgcttct tcccaactca ttttatctgg aatttttgct acttttgctg atggcaacac
    19321 gacttcttct ccgtaagtcc cgccgactgc atggacagct actcgatcac ctacatgaac
    19381 agttgtgaca tctgaaccaa tctctgtcac aataccagcg ccatcattac ctggtacata
    19441 agggaatttc aatgttcgta tctctttcat ttctcctaaa cgcaaggcaa catcataagg
    19501 attcacactg aatgctttta tttctacacg aacatgtcca gaaccgactt ctctgggatg
    19561 agcatcgatt tcttcaaaaa catcttgtgc ttctccgtaa ccgttaattg caaatcttcg
    19621 catggtcacc atctcccttt ttctttaagt ttacgtttat gcctttattt tgacaaaaaa
    19681 aatgtctttt cgttttggcg catgattcaa aacaaaatag ttgtcttttt tcgcgtaacg
    19741 tgttaaatta tttatctaaa agcgtaaaga ttacgattta cgctaataaa aaataattgg
    19801 aggaatttgt tgatggctaa aaaatcaaag attgccaaag caaaaaaaca gcaagaaacg
    19861 attgaacgct atgctgcttt acgccaggaa ttaaaagcgc aaaaagatta cgaaggcttg
    19921 cgtaaacttc ctttagatgc tcgtccagaa cgattgaaaa atcgtgattt aatcgatgga
    19981 cgccctcgcg gatacatgcg taaattcggg atgtcccgta taaaattccg tgaattagca
    20041 cataaaggtt tgattccagg agtaaaaaaa gccagctggt aatccaagtc tggctgtaat
    20101 gtaaaggagg agggcgcatg gaccactcct tggatcatca aaaaagatac gttcgcttct
    20161 tctagcagaa atgctggtac tagaagcaga cgtatctttt tttacgcaca gctggtcctt
    20221 ttttatatgt aaaggctgca tggttacttc gctatattta tttagtaaca ggagtcagtg
    20281 cttcgatacc cacactaatt ttcaatgctc ggtcaacttc tgccataatg ggtcgatcca
    20341 aatggcagac cttttctttc aatcttgatt tatcgatcgt tctgatctgc tccaacagga
    20401 taacggagtc tctttctatt cctgttccag tagctttgat cccaatgtga gtcggaagct
    20461 taggtttagc catttttgcc gttatggccg caacaatgat cgtcgggcta aaatggttac
    20521 ctagattatt ttgaataacc aaaactgggc gtgttccgcc ttgctccgat cccacgactg
    20581 gagaaagatc agcaaaataa atatctcctc tttttaccat aggatcctcc ttattcttga
    20641 tattctcttg gtatccgctg tcctaatccg caagccactt catagtggat cgtacctaat
    20701 tgatctgcta tatcctgcat agtgatttct ttgtctgcat ttttgccgat aagcgtcact
    20761 tttgttccga caggaaattc ttgaggcaat cggatcatca gttgatccat acatacacgc
    20821 ccaatcgttt cacagtagtt cccttctacc aatagagaaa aaccctgcat ctttcgtggc
    20881 cagccatctg cataaccgat cggtatcgtt ccgatccact ctgcttcagg agtgatatat
    20941 gtttctccat agccgattcc ttcacctgct gggagttttt tgacttgaat caattcagaa
    21001 accagctcaa gtgctggctg caatggataa acttccggta gtgcgtgccc agagggatta
    21061 agtccgtaca ttgctactcc ataacgaatc atattgccga ttgtttcatc atgccataaa
    21121 gccgttgcac tattgctgac atgtacatat cgaggaagtt cgggaagttt tttcagcact
    21181 tctataaagc gttctttttg cagattccaa tatgtatcat ctgcctgatc tgctgtagaa
    21241 aaatgggtaa ataaaccttc ccattctaat acacgacttt ctttgatgaa tgcagctgcc
    21301 tcttttactt cttcaggtgt acaaaatccg atacgaccca tcccagtgtc tgcttttaga
    21361 tgaatcgaaa gaggcgtctc tagcttactt ttctctaaaa cagcagctgc tgcttgcaac
    21421 cattcccgtg tacctgcagt tactgataaa tcatgagcaa cagctacagt cagtgcgtcg
    21481 taaggaacca tattcaagat aaggatgggc tgagtaaatc cagcttctct caactcgact
    21541 ccttcatcta aatttgaaac acaaaagccc gaagctccac ctgcaactgc tgcctcagct
    21601 gtttcaatag ctccatgacc gtatccgttc gcttttacta ctgcaaacaa ttcttttcct
    21661 tgcggaagtc ttgcaacttc atttgctaca ttttcagtta tagctttttt atgtatcact
    21721 gctttcgttg ggcggtgcca agcgacaacc atcgtttttc cttctttcta gtcttccaaa
    21781 ataatttgtg caaaagctgt ttcatttgta tgagtaatac tgacccatac attgccttca
    21841 tgaggtgaac gcgtcacaac aggctgacca ttttcatttt ttaaaatctc gacatcttga
    21901 aaggtaactt ttccgatccc agttccccaa gctttagaga aagcctcttt gcaagcgtaa
    21961 cgcccgccta aaaattctac ttggcgatga aaaggcaagg actggaatag tttcatttca
    22021 tcagaggtca agattcgtgc aatgaatttt ggtttttctt cgatcagccg agtgatccta
    22081 ggaagttcga ctgcatcgat cccaattcct tttatcatat gcttttctct tttcaataaa
    22141 tttagaacac tctcattcta ccaaattata accattttgc cacaaataag tccacaaata
    22201 acaaaaatga aagtttctct taaaaaagag aaactttcat tttatgttta atcgttattt
    22261 gaacggatta caaatccgcg ttttttatca ttgtgacgtt taccgccatc tttttggctg
    22321 ttattatttt tgctataacc gccttttgat ttgtttccac gataattacc gccgtcacga
    22381 cgattacggt tgtttccgcc accacgtttg ccatttttat tgtatccttt tttctgcatt
    22441 ggcaatgggc gttctggtgt gatcttcact ggcactgcat cagctggatc ttttgctgtc
    22501 gtttttagca aaagtgcaac taaatcttgt gcagaatatt cttctaataa tttatctgct
    22561 gtttgcaggt atttatctag accattttct gcaagttttg tttcgatttg ttcaactgca
    22621 gcacctaatt gacctttgaa tgcttctttt tcggttggtg gacgtaaagt cgtcatccgt
    22681 tttttcgtca agttttcaat cacatgcaag tagtccattt catttggtgt aacgaaagtc
    22741 acagacatac cgccttttcc ggcacggcct gtacgtccga tacggtgaac gtagctttct
    22801 ggatcttgag ggatatcata gttgtacaca tgagtgacac ctgaaatatc taatccacgt
    22861 gcagcaacgt ctgtcgctac aaggatatct aaatgaccgc tcttaaatga acgcaatacg
    22921 ctcattcgtt tttgttgaga caaatcgcca tggattcctt ctgcacggta accgcgtgct
    22981 tctaatccgc gtgccaattc gtctacacgg cgttttgtac gtccgaagac gattgttaat
    23041 tcaggtgttt gaacatcaaa caagcgagtc atgatatcga atttttcgta ttcttttgcg
    23101 cggacatagt attgatcgat caagtcagct gtcatttctt tcgctttgat cttcacatga
    23161 tgtggatttt tcatgaattt aacgccaata ttcttgatag ctggcggcat cgttgctgag
    23221 aaaagtaatg tttgtctttg atcaggaact tgtgaaatga ttttttcgat atcttccaag
    23281 aagcccatat tcaacatttc atctgcttca tccaaaacta atgtttgaac ggttccaagt
    23341 ttcaatgtgt ggcggttaat gtggtcaagc atacgtcctg gtgtacccac aacgatatgt
    23401 ggacgatctt tcaagccacg gatctgacgg ccaatatctg ctccgccgta aacggcttgt
    23461 acacgtactt ttttgtcgcg ccctaaacga tataattctt cttgtgtttg gatagctaat
    23521 tcacgtgttg gtgcgataac taaaccttgt aattcgtgac gatctggatc gattttttct
    23581 aacattggta atccaaatgc agctgttttc cctgtacctg tctgtgcttg tccaataacg
    23641 tcttttccag ctaatgctag tggaatcgtt tctgcttgga taggcgttgc ttcttcaaaa
    23701 ccagcgcgtt cgactgattt caacaattct ggtgataatt ctagttcttt aaatttcaaa
    23761 tgaatcctcc tatatggttg ttaactgagg atgtagcttt tttgctaccc cgtcgatttg
    23821 ttcggtcaaa gtgtattgtt tctttttgac agtggccacg cacgttttta tccattctta
    23881 gcccctgtgg caccaccgaa aaatcacttt gcgaagttta ggcattagaa aagccaaacg
    23941 ggtcgcgttc agctttatca ttattatgac atctggcttc aacaagttgt ttctatggca
    24001 aaaccagtcc aatttctctt catctttcgg caataggtta ttctaacata gtttttcgtt
    24061 ttctgcaagt ttacagattt ttatttcatt tgctgaagtt gttctaccac ttcaaataag
    24121 cccatgctgt tgctgccttt tagcataata ctatctgttg cttcaagatt ttgtttgata
    24181 gcatcaatca atttattctt ttctttttca aaatagaaca caggtagttg ggggtatttt
    24241 tggttcaatt cttcttttag tgcatgcatt tgttccccat aaaggaaaat catggcgaat
    24301 ttctcaggat cgatatgttc actcatttgc gcatgcatat ttaaagactc tttccctaat
    24361 tccagcatat ctgctaacac agcgattctg cgtccttttg ttggcagatt tttaaaagta
    24421 tctaatacca gtcccatggc tgttggattt gcattgtaga catcgctcaa aatagctgca
    24481 ccatttcctg ccgttagcca ttcggttctg ttttgagtga tagaaacatg ggcgagccct
    24541 tggcgtatct caggtatgga cagtccaaag aagcgcccga tcgtatacgc aatcaaagca
    24601 ttcgttacat tataacttcc aggcagcggg atcgtaaatg tttctccttc tactacaaaa
    24661 tcggtttgtg cttttccttt tgctgtcact tccgcatgaa catcgccatt tccaataccg
    24721 aatgttgtga ctgtttgtac taatgttgcc gttaaaggtg tcagcaacgg ttcatctgcg
    24781 ggcacaacga gtaatccgtc tgatacaagt ccgtcagtga tttccatttt ggctttagcg
    24841 attccttctc gcgacccaag attttctatg tgtgcttcac cgatcattgt gattgctgca
    24901 gcttttggtt gagccataag tgaaagttcc gtgatttcat tggcgtgatc catgcccatt
    24961 tccaaaacga tcttttctgt atcttggggc atgtgcaaaa tcgtgtaagg aagaccaata
    25021 ttattgttgt aatttccttg cgtcttatat gttttgtatt tttcagacaa gacagacgcg
    25081 gttagatctt tcgtggtggt tttaccattg cttcctgtaa ttgcaacgac ttctgcgcct
    25141 actttttcca agtaataggt tgccaattgc tgcatggctt tcaaaggatc tgctacagga
    25201 ataactggaa aattttttgg agcttgttca acaggcaagc tccaaaatga agcagatgct
    25261 ccgtttgtga tcgcttgctg gataaattca tgtccatctc gcgtaccagc aagaggaacg
    25321 aataaacttc cttcggttat cttccggctg tcaaattcga cattggtaat ctttgtcagt
    25381 gcagagcatt cgctgcccac ggcttgtgca atttcttgta ttgttagatt cattgagatc
    25441 tctcctaaaa aaggttgtga aaaaagcgtt tggcctaaac ggcgggttcg catgactctc
    25501 acttccaagt aaatttctct attcgtattt agaagctaga atgaacgcgg ttacgcaatc
    25561 actccagcct cttctttatt tctctttatt catttttttc aaaaaaagat tgacgctgat
    25621 ggtatcgatt cattcctaac tggatcagtt cttcaatcaa atcaccgtat ttcaagccca
    25681 tattttccca taagagtggg tacatactga actccgtaaa tcctggcata gagtttaatt
    25741 cattcaggaa taattcattt ttatttgtca aaaagaaatc gcaccggctc aatccgcttc
    25801 cacctaacat cgtgtaagct aacttcgcgt actcttgcgc tttttgataa acttcttctg
    25861 gcacttcggc tggaatctgc atttcgattt tattattgat atattttgct tcataatcat
    25921 agaatgctac gtcttttacg acttcaccag gcaaagtcgt ccgaacgtct tcatttccta
    25981 atacagcaac ttcgatttcg cgcgcttcaa ttccttgttc aacgattgct cgagaatcat
    26041 actgataggc tgttgctaaa gcattttgca gctcttctcg gttttctgct tttgtaatgc
    26101 cgacactaga acccatattc gccggtttga caaacatcgg ataaagcaaa gaaccttcac
    26161 attgatcaaa tactttttta ggattttctt tccattgatt cttaagtact ggtacataag
    26221 gaacttgcgg cacaccagca gcttgtaaaa tatacttggt catgatttta tccatggcac
    26281 atgcactggt caatacgcct gcgccgacat aaggcatatt caatgtctct aagaagcctt
    26341 ggatcgttcc atcttccccg tttggcccat gtaaaactgg aaaaacgatg gctccttctt
    26401 ctttgatttc gcccggattg atcacttttc ctgtaaagcc ttcctctgtc tgtccacttg
    26461 ggtcccatga aagatgcaag acatctttgc tggcaggttt ttctgttagt aatggacctt
    26521 tgacccattg tccttctttt gtaataaata cgagttgaac ttggtaataa ttataataaa
    26581 tggcatttaa aactgaaaat gcggaaagaa tggacacttc atgctctgcg ctgcgtccgc
    26641 catatagtaa agtaatcttc aaaagattgt cctcctgtat tctaaatcgt tcagtgattt
    26701 actctcatat tttagcacaa aacaaaaata aacgaataac ttatttataa aaaaacgagc
    26761 cagcttctaa aagaacgagc gtcatttatt ttttgtcagc tagattattt gtcccgtttt
    26821 ttataaaaag ttatgataaa gaaaaggagg gatgatgatg gcgcaaacaa atccacgcag
    26881 ccgcttggca gaaatcattc gagtgttcat caattacaat gtagtgccaa actttgtaca
    26941 gcaaaaaaat cccgaacagg taaaaaaagc ctttgaagaa ctaggcccca ccttcatcaa
    27001 gataggacag atgctttctg ttcgagaaga tttgctttct tctgccttca ctcaaacatt
    27061 caaaacatta caagatagtg tacctagcga cacattttca acggtcaaaa agacgatcga
    27121 aacagaactt tctctttcat tatcagatat attcgatgat ttttcaaaat cgccttttgc
    27181 ctctgcttct atggggcagg ctcatcgtgc caagctgaag aacggtgacg cggtagtcgt
    27241 taagatccaa caccctaaca tcgccgaaga gatccgactt gatcttcaac tatttgaacg
    27301 agcgatccct ttgatcaaat acattcctga aactagcgta gttgatctaa aaggggtact
    27361 ccaagaagta aaacgttcct tgatcaatga aatggacttc ttaaaagaaa gtcaaaatgg
    27421 cgaacaattc tatcaaaaga acaatggctg gaaagagatc cgcagcccca agatttatga
    27481 cgctttttgt tcgaaaaaag tgatcgtgat ggaagaaatg tctggtaaaa acttgaatca
    27541 tttaatgaac gccgaaaaca aaactgaaac ttttatcact ggaattcaaa acaagcagct
    27601 gaaacaagaa gttgcaaagc ttttagttga gaactttatg aaacaggtgt ttgatgatgg
    27661 ttttttccat gctgatcctc atccagggaa tctgctattc catgtactaa caaaagaaga
    27721 acagacacaa gctagccgaa aaactgaaac agtccatgaa aaagagttcg gttcctttgc
    27781 ttttcgagcc tccacttctg cagaagaccc tgttgcgcca tataccatca actacatcga
    27841 ttttggtatg atggggcatc tttctgcagg acttaggcaa aaactcacac aagccgtttt
    27901 agcgctgtat acgaaagatg cttatcgaat cgaaaaagcg gtccttcgac tttgtcagca
    27961 agaaggctcg tttgatgaaa gtcggttcca ccaagaactg acaagttttc ttgaacagta
    28021 ttatgattct cctattgatg aaatcaactt gcaagaagta ttcacacatg tcgtgacgat
    28081 ttgtcatcaa aataatctgc agttcgatcg tgatatcacg cttttgctga aagcatttgg
    28141 gacattagaa ggggtgatcc gcgtcctcga tcctgaagtt tccctcatgg aagtcgcttc
    28201 gccttttgct caacattatt ttctcactca tctggatgta gaagatacat tgaaacaaag
    28261 cggattagac ttattggaag ggatgaaggc tgcgcctaag atccctcaac agctccacca
    28321 tctattagaa atgtggactt cgggacaagg aaaagtcaat ttggaattga aaaagcaaga
    28381 caaattattg agcaggatcg aaagtatgat caatcggctt gttttcggta tgattttagc
    28441 tgcattgatc gtagggtcct ccctgcttgt ccaagcagca cctgtagaaa acgcagaggt
    28501 cgtttcctta ttagggatat ttacgtatgc tattgccgca ttcgtcatta tttttttggc
    28561 aatcgatgca ttgatccaaa tgtataagaa gaggaaaaag tagaaaaagg ttgtgacaga
    28621 aagaatcagc ttcaagaaat aagaaggggt tttacataaa ttgtttctca aatttttgtg
    28681 aaaattcggc ttatttttcg aagaggctac ttctgaaaca ccgagaataa aaaatgtgaa
    28741 aaagctgttc tgcacccagt aataagaaca atcaaaaagg gaacatggaa cacatttatc
    28801 ctgtttccct gttccctttt tgatgtattc gagcataaat aaaatacctt cgttacctac
    28861 tatcttctgt acttggtaaa ccttagtcaa ttgccttttt taacgcttga cgaaaagaca
    28921 tatgatcttt ctccgtaaag ggttttggac cctttgtcct tatgccagct tttcttaact
    28981 ttccactatg ataacgttgt tctagaagcg tgccgatatt cgcttcgtca aattcttttc
    29041 cagagtgatg gaacactcgc acacccttcg gcaatacaag tgaagctaaa ccataatctt
    29101 gcgtgatcaa aaagtcattc ttcttaacca taccgacgat tttataatcc gcactatcag
    29161 ctccgcggtc tacgtagacg atttggacat gattagcgta ttctttggtc gtataatggt
    29221 ccacactggt cacgataacc acgtcaagac cataggattc tcctaatttt atgacatctt
    29281 cttttactgg agaaccatct ccatcaatga atagcctcat tctttttcgc cttcttccat
    29341 aaatgataaa cagttactaa tccgctcagt aaaagaccaa cattgatcca ttttgccaag
    29401 ataagcagtt gtacactttc tatcggcact gattcatggt tcagcgtgat caataataga
    29461 tggattggct ctcccatttc ttttgctaaa acaaacaaaa gcgtcatcaa taaaaactgg
    29521 tggatcacac tccctttatt ttgagtagta tacttgacaa cgatccggcg atccattgga
    29581 atagctgtat cagtttgata atattcctta tttagccagc tgcctgttac tacgtgccag
    29641 aaactaagca gaaagacacc tatcgcaaaa ccatttggaa tcaagaggac caataaagaa
    29701 gaactcagtc caagcaattg catagccaat ggagaccatg ttaaacgttt caaaatccat
    29761 ggtccgacta tttttgctgc aatcaagcct gctgcataag gcaaatacat aaaaatcgaa
    29821 agtttttcta atccgtacag tatgccaaca taaaaactgc taaataaaaa aaggaaattc
    29881 cctagcattc catttagaaa agtaaacaga tttagccagc ttggcaactt ccattccttc
    29941 ttctggtgtc ctaaaacaaa cacgaacaag atgaacagca gcaagaaacc ataaatagca
    30001 agatcaaaaa gcctttcatc gaataacaat cgggcatttc tcaaaagaaa caataagcca
    30061 aagaatatca aaaataaaac aagctctcgc gtagctacca gatttctttt gatgtctttg
    30121 aaatccagtt cgtaacgcgg ataatgacta accgtatggt aggccatgac gtaaaaaagt
    30181 gtataaatag taaagcttgc tgcaatctga gtcggtgcag gcaactcaat cgcttgatac
    30241 aggagaaata gccagatagc tgcaaataag tactttttcc catccatatt gataaaacct
    30301 tgcgtctttt cgtgaaaatt gaccgtggta ttagcaggta caagccatgc tgcactcagc
    30361 cctagcaaaa cacttgaaaa ataaaataaa ggaaaataaa atacgcctaa tattccaagc
    30421 aatgaaccag caccaccaat cagcagagcg atcatcagca acgtgtaact atctagacca
    30481 aatcgtagac tgttgactaa aaatagtcca gtcatccgaa aggcataaaa cagcgtaaat
    30541 ggcaaaatcg tcagccaatt atgcgagtct gttagtccca aaaataaaag ataaggaata
    30601 aaaataaccg tgttacttaa gataaagggc gttgtttgtg taaaattacg cccaaatcgt
    30661 ttgagttttt cgaa
//
LOCUS       DARDHA010000016        29998 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000016 DARDHA010000000
VERSION     DARDHA010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.016"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..144
                     /locus_tag="SDA10_000619"
     CDS             <1..144
                     /locus_tag="SDA10_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239549.1"
                     /translation="YVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVL
                     TEL"
     gene            286..1149
                     /locus_tag="SDA10_000620"
     CDS             286..1149
                     /locus_tag="SDA10_000620"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEG4239550.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            1394..2332
                     /locus_tag="SDA10_000621"
     CDS             1394..2332
                     /locus_tag="SDA10_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEG4239551.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            2355..3035
                     /locus_tag="SDA10_000622"
     CDS             2355..3035
                     /locus_tag="SDA10_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG4239552.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            3008..3832
                     /locus_tag="SDA10_000623"
     CDS             3008..3832
                     /locus_tag="SDA10_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG4239553.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            3999..4817
                     /gene="purR"
                     /locus_tag="SDA10_000624"
     CDS             3999..4817
                     /gene="purR"
                     /locus_tag="SDA10_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEG4239554.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            5320..6693
                     /gene="glmU"
                     /locus_tag="SDA10_000625"
     CDS             5320..6693
                     /gene="glmU"
                     /locus_tag="SDA10_000625"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEG4239555.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            6787..7758
                     /locus_tag="SDA10_000626"
     CDS             6787..7758
                     /locus_tag="SDA10_000626"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEG4239556.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      7764..7866
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7932..8501
                     /gene="thiT"
                     /locus_tag="SDA10_000627"
     CDS             7932..8501
                     /gene="thiT"
                     /locus_tag="SDA10_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEG4239557.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(8582..9355)
                     /locus_tag="SDA10_000628"
     CDS             complement(8582..9355)
                     /locus_tag="SDA10_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239558.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            9622..11322
                     /locus_tag="SDA10_000629"
     CDS             9622..11322
                     /locus_tag="SDA10_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEG4239559.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            11292..11510
                     /locus_tag="SDA10_000630"
     CDS             11292..11510
                     /locus_tag="SDA10_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEG4239560.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            11530..12420
                     /locus_tag="SDA10_000631"
     CDS             11530..12420
                     /locus_tag="SDA10_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEG4239561.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            12438..12722
                     /locus_tag="SDA10_000632"
     CDS             12438..12722
                     /locus_tag="SDA10_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239562.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            13209..13823
                     /gene="gmk"
                     /locus_tag="SDA10_000633"
     CDS             13209..13823
                     /gene="gmk"
                     /locus_tag="SDA10_000633"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEG4239563.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            13828..14133
                     /locus_tag="SDA10_000634"
     CDS             13828..14133
                     /locus_tag="SDA10_000634"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG4239564.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            14386..16794
                     /gene="priA"
                     /locus_tag="SDA10_000635"
     CDS             14386..16794
                     /gene="priA"
                     /locus_tag="SDA10_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEG4239565.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            16809..17300
                     /gene="def"
                     /locus_tag="SDA10_000636"
     CDS             16809..17300
                     /gene="def"
                     /locus_tag="SDA10_000636"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEG4239566.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            17293..18231
                     /locus_tag="SDA10_000637"
     CDS             17293..18231
                     /locus_tag="SDA10_000637"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEG4239567.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            18231..19589
                     /gene="rsmB"
                     /locus_tag="SDA10_000638"
     CDS             18231..19589
                     /gene="rsmB"
                     /locus_tag="SDA10_000638"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEG4239568.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            19602..20342
                     /locus_tag="SDA10_000639"
     CDS             19602..20342
                     /locus_tag="SDA10_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEG4239569.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            20339..22408
                     /gene="pknB"
                     /locus_tag="SDA10_000640"
     CDS             20339..22408
                     /gene="pknB"
                     /locus_tag="SDA10_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEG4239570.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            22573..23472
                     /gene="rsgA"
                     /locus_tag="SDA10_000641"
     CDS             22573..23472
                     /gene="rsgA"
                     /locus_tag="SDA10_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEG4239571.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            23485..24135
                     /locus_tag="SDA10_000642"
     CDS             23485..24135
                     /locus_tag="SDA10_000642"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEG4239572.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            24136..24774
                     /locus_tag="SDA10_000643"
     CDS             24136..24774
                     /locus_tag="SDA10_000643"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEG4239573.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            24929..25783
                     /gene="mreC"
                     /locus_tag="SDA10_000644"
     CDS             24929..25783
                     /gene="mreC"
                     /locus_tag="SDA10_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEG4239574.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            25787..26296
                     /gene="mreD"
                     /locus_tag="SDA10_000645"
     CDS             25787..26296
                     /gene="mreD"
                     /locus_tag="SDA10_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEG4239575.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            26551..28125
                     /locus_tag="SDA10_000646"
     CDS             26551..28125
                     /locus_tag="SDA10_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEG4239576.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(28193..29992)
                     /locus_tag="SDA10_000647"
     CDS             complement(28193..29992)
                     /locus_tag="SDA10_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEG4239577.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         9944 a         5400 c         6430 g         8224 t
ORIGIN      
        1 tatgtcgatc attgggaagg acgttatttt gcttatagga aaggaagtaa tttcggattt
       61 gctaccatgc agctactcag tctgatagga aaagaaaaag gaaatgttcc gagtttggca
      121 gaagaagtgc tcacagaatt ataagaatca ctagaacttg tttggattat gaagagaaaa
      181 ttctgtgaaa gctgttgaaa cgatgacaaa atcaaccact aggcagacat aagctatact
      241 tttatttttc cctatgatag aatacttgcg acttagactt ttcgaatggg tgagaggatg
      301 gaattgattg aaaaagcgcc agccaagata aatttaggac tggatgtatt atatagacgg
      361 caagatgggt atcatgagtt ggaaatggtg atgtcgagcg tagacttggc agatcatcta
      421 ttttttgaag aaataacaga agacaaaatc attgtagaaa caaataaagc atttttaccg
      481 gtagatgaac ggaacaatgt ttatcaagct gctttattgc taaaaaataa atacaatatc
      541 aaaaccggtg tcaagatctt tgttaagaag aatattccag tagcagctgg attaggcgga
      601 ggaagtacag actgtgcagc tgctctaaga gggatcaacc aattatggaa tctgggctgt
      661 tcgttagaag aactggcaga aatcggtttg gaagtcggta cagatgttcc ttactgtgtc
      721 tacggaagta cggcatttgt cggtggcaaa ggggaagaaa tccaagtatt accttctatg
      781 ccacagtgct gggttgtgtt ggtcaaacca agaatgagtg tctctacacg tacagtgttt
      841 agagaagtag atacgcaagc catccaacat caagacatcc acggacttgc tgaagccatc
      901 cgagagcaag attatcagca gatgatcaaa aaaatgggaa acagtttaga agatataacg
      961 ggaaagcggc atccagtcat ccatcaaatc aaagagcgta tgatcaagta cggagcagat
     1021 gctgcattga tgagtggaag cggaccaacc gtttttgcct tgtgtcaaca ttattcaaga
     1081 gcgcagagag tatataatgg gttaaaaggt ttttgtgagg aagtttattt agtacgaacc
     1141 ctgcaataaa atataagaag taaaataggt gatgaaaaac tgaggaatcc taaaatggta
     1201 ttcttcagtt ttttattgta gtgaaatggc tagatatgag cagcaactca tccattttac
     1261 gatttaatca agaatataca tgcttaaatc agaaattgat ggatttctat tgactgtgtt
     1321 tttttatttt gatacattag ttcacgttaa taaatcgtaa aattccgatt tgttcattga
     1381 aaggagcata taaatgaaaa aatatctttt gacagctacg gtaatgatag gtgctttact
     1441 gtttgcagca tgtggaaata cgaacaagga agcagacaaa aaagaggatt tgacgatcgt
     1501 tacgactttt tatccgatgt atgattttac aaaagagatc gtaggggatg aaggaaacgt
     1561 gaaacttttg atccctgctg gaacggagcc gcatgatttc gaaccaagtg caaaagaaag
     1621 agcagaaatt tccgatgcag atgttttcgt gtataatagt tcagacatgg aattttttgt
     1681 ggattcgtta aaggattcag tggatagcaa acagaccttg atgatcgaag cagctaaagg
     1741 aatcgaccgt ttagaatcgc aagaagcaga cgaacatgaa gaatcagaag aagggcatgg
     1801 tcattcacat gaatacgatc cacatgtttg gctcgatccg gtattagcta taaaagaagt
     1861 cagaacgatt gctgaagaat taggcgaaaa atatccagat aaaaaggaaa cttttacgaa
     1921 gaatgccgat gcttatatca aaaagcttga agcactggat caaaaatact ccgatgagct
     1981 aaaagatgca acaaaccgca catttgtcac tcagcatgct gcttttgctt atttagcgaa
     2041 tcaatacgac ttaaaacaag tagcgatttc gggcgtttca ccagatcagg aacccacacc
     2101 aagtcgctta gctgagctaa aagaatttgt gaagaaaaac aacatcaaag tgatttattt
     2161 tgaagaaaat gcctcttcga aggtagctga gacattgtcg aatgaaacag gagtgaagct
     2221 agaagtgttg aatcctttag aaagcttgac taatgaacaa atcaaagcag gcgaaaatta
     2281 tatttctgtc atggaaaaaa atctgaaagc tttaaaagaa agtatcaatt aagttttaga
     2341 atggaggttg taaggtgcgt tatatcgaaa tatctgattt gaccttttac tatgatgatg
     2401 aacctgtact agaagatgtc tcttatcatg tagatgcggg tgaatttgtc attttaactg
     2461 gagaaaatgg agcagccaag tcaaccttgg tgaaagcatc gttaggatta ctgaaacctt
     2521 caaaaggaat gataaagatt gctgagaaaa atcaagaagg aaaaaaatta agtatcggtt
     2581 atattcctca acaagttgca tctttcaatg caggttttcc tagtacggtg atcgaattag
     2641 tccgttcagg aagatatcca agaggccgct ggttcaagcg tctctctgaa aaagatcatc
     2701 aacatgtaca gcgtgcttta gaagctgtag ggatgtggga tatgcgaaat cggcgaatag
     2761 gtgaattgtc tggcggacaa aagcagcgaa tcagtttagc gcgtattttt gctactgacc
     2821 ctgacttatt tgttcttgat gaaccaacaa caggtatgga tgaagaatcc agaaatgaat
     2881 tttatcgtct tctccgacat agtgcgcatg tccatgggaa atcagttcta atgatcaccc
     2941 atgatcatga agatatcaaa cagtatgcgg acagacagat ccgactggtg agaaaggagg
     3001 attctcaatg gcgttgcttt catatgaatt catgagaaga gcgtttcttg ccgctatctt
     3061 cattgccgga atcgccccga tgctaggtgt gtttttagtg atcagaagac aatcgctgat
     3121 ggctgatacg ctttctcatg tctctttagc aggggtagcg ttaggattct ttttgaacct
     3181 taatcctaca cttacgacca tgctagtcgt tgtattagca gctgtgatat tggagtatct
     3241 gcgaacgatc taccgaacct attcagaaat ctccattgcc attttaatgg ctggtggttt
     3301 ggcgctagcg ttagtattga tgaatttgag cggagggaat tcagcaacaa gtatccaatc
     3361 ctatctgttt ggttcgatcg tgaccattac atgggagcaa gtcatctttt taggaatctt
     3421 attcgttgcc atcggtatat tgttcttttt attcaaacgg ccgatgtatg ttctgacttt
     3481 tgatgaagat accgcccacg tggatgggct acccgttcat ttgatgtcga tgatgttcaa
     3541 tgtgattact ggtgtggcta ttgctgtgat gatcccgatt gccggtgcac ttttgatatc
     3601 cgcaatcatg gttcttcccg cagcaatcgg tatgcgatta ggcaaaagct tcaatgtcgt
     3661 gattgctatc agtgtttgtg taggatttct agggatgctg tcaggactaa ctagctcgtt
     3721 ttatctagat acacctccag gagcaacaat cacattagtc tttattgctt tgttcctgat
     3781 tgtaaatatc atcaaacgaa tattggttgc cttcagaaga aaaaataact aaattataaa
     3841 gaaaagaagc cttataaaaa cgaaaaattt tttataggct tctttttatt ttaataaata
     3901 ccgaactatt ttcttatttt ttcataaaat gctcgaattt ttgaagaaaa acttatataa
     3961 tgtaaaacga taaaatttct taaaaggagt tggaataggt gaaagtacgt cgcagtgaac
     4021 gtctagttga catgacacag tatttattga atcacccgca tgaattgatt tccttaacaa
     4081 gctttgccga acgttatgaa tctgctaaat cttctatcag tgaagatttg ggaatcatca
     4141 agaaaacatt taaagaacga gggtatggaa ccttggaaac aatcccgggt gctgcaggtg
     4201 gtgtattgtt catccctgaa atttcatatg aagaggcgaa aaaatatatt gaatctctag
     4261 cagaacgtct atcagaacaa gaccgtctgc ttccaggagg atatgtttat ctctctgatt
     4321 tgttaggaga acctgaactg ctgcgtcaag ttggaagaat cattgcttct aaataccttg
     4381 gtgaaaaaat cgatgctgta atgactgtag ccacaaaagg cgtacctatt gcccaagctg
     4441 tgtcctatta tctgaatgcg ccattcgtta ttgtacgccg ggactcaaaa ataacagaag
     4501 gatcaacagt aagtgtcaac tatgtgtctg gatcttctga aagaattgaa aagatggaac
     4561 tgtctaagag aagtttgaaa cgtggttcac gtgtccttgt agtggatgat ttcatgaaag
     4621 gcggcggaac agtcaacgga atgaagagct tgattgaaga attcgaatca gaacttgttg
     4681 gcatcactgt atttgccgaa tcaaaattca acggtcatcg cgccatcgat gattatacgt
     4741 cactgcttta tgtcaaagat gtagatactc aaacgaaaaa tattacggtt gtgccaggaa
     4801 attatttttc agaataagag gttgtgaaaa agccgcttag tttcgagtag taagaaaaat
     4861 tttgtgaaaa tggctttcca cattttttca taaatttggc ttaataccga gaaactggct
     4921 tttgaacacc gtttatctag aggttgtgaa atcagcgtcc tgtcctgagt attagctcaa
     4981 taaacggaaa aattgctcct catattttgc cgtttattga gctaataccg aaggtcagct
     5041 gattgaaaaa cgtttaatga agaggttgta agcctaccgt ttaactctaa gtcacaagaa
     5101 acgatttcaa aaaacagctt ttcatgtttt atgagtagtg tgacttgtga ccgaggagtt
     5161 ggcaggagag caccggttac caaatagata ggcccgttat ggtctatcta tttttttatc
     5221 cagaaaacgc tgggataaaa gaaaatcact tgttttgttg aaaaaatggg tatactggga
     5281 taaactgaaa tcagcaaact atatttagga gtgacgcagt tggacgcacg ttatgcaatt
     5341 attttggcgg cagggaaagg tacccgcatg aagtcaaaat tatataaagt tttacatccc
     5401 gtttctggta aacctatggt agaacatatc attaatcggg tcagtgaaac gaaaccagat
     5461 gaagtgatta cgatcgttgg acacggagca gaacaagtta aagcacaact aggagaacgc
     5521 agtaaatatg cgttacaagc agaacaattg ggaacaggac atgctgtttt gcaagctgct
     5581 tctttcttgg aaggaaaaaa aggaacaacg ttagttatca gtggcgatac accattattg
     5641 actactgaaa cgctgaacaa tctttttgaa tatcaccaag gaaaaaatgc aagtgcaacg
     5701 atcttgacgg cacaagcaga aaacccaaca ggatatggac gaatcatccg tgaccatatt
     5761 gggatcgtag aaaaaatcgt ggaacaaaaa gatgcaacac cagaagaagc tcttgtacaa
     5821 gaaatcaata caggaactta ctgttttgac aacgaagctt tgttcgatgc gttaagcaaa
     5881 gttggaacaa acaatgctca aggtgaatac tatctaacag atatcatcga aatcttaaaa
     5941 gaagaaggcc atacagttgc tgcttatcaa acggatgatt ttgaagaatc aatgggagtc
     6001 aatgatcgga tcgcgttagc taaagcaaat gagatcatga gaaagcgaat taaccagatg
     6061 catatggtca atggcgtaag ctttgtcgat tcagcaacga catacatcga tgcaggcgta
     6121 gaaatcggtc cagacacatt gatcgaagca ggtgtacaaa tccaaggaaa tacagtcatt
     6181 ggttctgatt gtgtcatcgg gtcacattca aaaatcgtag acagtaggat tgaggatcac
     6241 gtcgtcattg aaaactcagt cattgaaagc agccatgtca agaaacatgc agatgtggga
     6301 ccttatgcac atttacgacc aaaagctgaa attggcgaaa acgtccatat cggaaacttt
     6361 gtggaagtga aaaatgctca aatcggcaaa ggaactaaag tcggtcactt gacttatgtt
     6421 ggtgatgcta cgctgggaga agaaatcaat gtcggatgtg gtgttgtctt cgtcaactat
     6481 gatggaaaga acaaacatca tacgactgtc ggcgaccaca gctttattgg ttctagcacg
     6541 aatatcatcg gacctgtcga ggtagctaaa aacagttcga ttgctgccgg gtcaacaatt
     6601 acagacaata ttccagaata tgcgctggca attgcgagag caagacaagt caataaagaa
     6661 ggatatgcaa aaaaactccc ttatctaaac taagactttt gcaaaatcac ttgatttatt
     6721 taaaaaatat gactactatt tattaggtag aattctagag ttgtgaaaaa gaaagtggag
     6781 gtccccatgt caaaacatta ctttgaccca agattaaaaa tttttgcgtt aaattccaat
     6841 cgcccattag cagagaaaat tgcagcagca gtcggcgtcg agttaggaaa atcatctgtt
     6901 actcaattta gtgatggaga aattcaagta aacattgaag aaagtattcg tggatcgcat
     6961 gtctatgtta ttcaatcaac gagcagccca gtcaatgata acttgatgga gttgctgatc
     7021 atgattgacg cactgaaacg agcaagtgca aaaacaatca acgtagtcat gccttattat
     7081 ggctatgcgc gtcaagaccg taaagcacgt gcacgggaac caatcacagc gaaattagta
     7141 gcaaacatga ttcaaaaagc tggtgcaaca agaatgctga ctttggacct tcatgcagtc
     7201 caaatccaag gtttctttga tattccagtc gatcatttga tgggtgcgcc acttattgcc
     7261 aattatttct tagaaaaagg aatcaaaggc gatgatgttg tcgttgtttc accagaccat
     7321 ggtggtgtca cacgggcacg taaattagcg gaatttttga aatctccgat tgcgatcata
     7381 gacaaacgcc gtccaaaagc aaatgttgca gaagtaatga atatcattgg acaagtcaaa
     7441 ggcaaaactt gtgtactgat cgatgatatg atcgatacag caggtacgat cacattggct
     7501 gccaatgctt tgaaagaagc tggtgcaact agtgtgtatg cttcatgtac acatccagta
     7561 ttatccggcc ctgctttaca aagaatagaa gattcagcta tcgagcgtct agttgttaca
     7621 gactcgatct atcttccaga agatcgtaaa atcgacaaaa tcgatgaagt aagtgttggc
     7681 ggactaatgg gagatgcaat caaacgtatc catgaaaaca aaccagttag tccattgttt
     7741 gaaacaaaaa ataaataata aataaactca caaaggggag tccaattggg ctgagattga
     7801 atctatttct aaacccttcg tacctgtatc ggttatgcga gcgtaggaat tgtgaataaa
     7861 cagctgtttg actattttcg aaagctgctt cctcctttgt gactttttta ttcaaaggag
     7921 gaagtttttt tatgggaaaa aatagagttt gggtagaagg aacgattgtt gcagcgttag
     7981 cgatggtgtt gtcgttgatt ccgatccaga tcggcagcag tttttctatt tcattagggc
     8041 agattccatt gactcttttt gcgttaagaa gagggacaaa ggcagggatg ctggcaggtt
     8101 tcatttgggg catcctacat tttccgttgg gacaagttta ttatttaagt gtcgttcaag
     8161 tgttgattga gtacccgatt gcctatactt ttgcaggcgc agcagggctc atggcttcaa
     8221 atgtccaaaa aggctatgta gacgataata cgaaacaaat tagaaaaagc atcgtgtttg
     8281 gtgcattgat tggcgcactt tccagatatt tttggcattt tattgcagga gtggttttct
     8341 ggggagcgta tgcactatgg gggatgaatc cttggctctt ttctttcgta atgaatggtg
     8401 ccagcgggtt gagtacagct attgtaacga tcatcgtttt attagggatc caacaaacga
     8461 ctccttcgat ttttgtgcca gataaacgac aggagctgta aataaaaaag aagcaagaaa
     8521 cttgagacag aatcatgtcc tagtttcttg tttccttttt attttttgat aagaaataga
     8581 tctacttcaa tggcagataa gaaagatcgg catcttctac caattgccag tgtcgatcgg
     8641 tgacagagta gattaaagta gcgatgattt cctcttctcg ataccgggtg ccttttaatt
     8701 tccaaatata atcttcaatc acatagattg ggatacgata tttaccatcg atcaaaccaa
     8761 cttgatcaaa agaaaaaatg atcccttcct gcatcaaatt acgcatttct ttcaataatt
     8821 ccacactgat tttttcaaca ggtgtttctt ttttgaaaaa agattttttt acattgatct
     8881 tatctgacaa gatcgaactt aatttactag caaaaatcct ataaaattca tctaaatagc
     8941 gataataaat ccgattcagc tgatcattaa gatcgaagta tacaaaatta ttctgtagtt
     9001 tataaaaaaa cggcgaatga agatgtgttt tcatatgacc aaaatataat aattctgaaa
     9061 tttctaaagg tgtcaattct ttcagcatcg ttaaatcagt aaagtcgatc cacttcaatt
     9121 catcgaatcc acgcctgtta cgagaagagg aaaagaaacg ctgaatattt tctgtaccac
     9181 ggattacttt cagccctgta tgggattcat attctccagc agaagcagat ggatcaagca
     9241 agagcaagtt ttgaggaaaa tgcacgatac tgtttgcaaa gtcagtagca gtcagccctt
     9301 ttgttaaaac agcattactt gtggtatcaa tatgtacata aactgactca gtcatcatgt
     9361 tcctcctctt atttcctctt aatctctttt attttacaac atttgcatta aaaaagcttg
     9421 tttcttttat tttaaaattc cattacaaaa tcacgttttc attgaaaaca ctctcatttt
     9481 caattaattt ttgtgatttt tgtatttttt tatttaaagg aggttttatg aattgttttc
     9541 ttatggaatc tacaaagtcg aatacgagac tcatgacctt tttatgacgt actggtatac
     9601 ttgaagaaaa aggagagcgc gatggagata aaaaaagttt acttttcaca gaaaatgtcg
     9661 ctgtattcgc ctatccagca agcaaaacaa ccccttccat tttttcaaag tcatcaagaa
     9721 agtagagacg ctttttggcg ttctgcaaaa aagcaatgtg cttttaaagc agtatcgaaa
     9781 aaatataccg tctattttac tattgctaaa agtagaggag atgttatttt taataatctg
     9841 ttgattgaag gtgaaacttt tgaatggaaa aaattttttc ggtatatgga agcttgcgct
     9901 cgttttttta ttaaagaaaa ctgttgtttt ttatttcaat ctcctctatc agaagaatgg
     9961 ttgcgtattt ttcataaaaa cggctatcaa ggaaccacac aattaaataa aaagctggtc
    10021 tatcatacag cacttgtttt aggtggagga ggagcgcacg gtgcttatca gattggtgtg
    10081 tggcaggcgc tgaaggaaca tgatatcaac tttgagatta tcacaggtac ctcggtaggt
    10141 gcgttgaacg gcgcattgat tttacaagga gatatgaaaa aagctgtgaa tttatggaaa
    10201 aagctgtcga caagacaagt gcttgcgtta ccagaaatgg ctgcagttga agaccttcgt
    10261 gaacgcttct atcgagaaag acgccaaatg acgaaaacag cactaattga aggcggggtt
    10321 tcttctgctc cattggaaaa gatggtcaaa gacaatttgg atttgtcctt actcaaacat
    10381 aatcccaaaa tacgtttata tacggtgtct actaaactgc cagaactagc ggaagtagtg
    10441 actgatatcc aacagacaaa aacagaagaa ataccagatt ggatactcgc ctctgcttca
    10501 ttttatcccg ctatggctta tcgaaagatc ggaaaaaata aatatgcaga cggtgggtat
    10561 cgcaacaaaa tacctattga tattgcgatt aacaaaggag caacagaggc atttgtcgta
    10621 gatgtgcaag ggccaggccc agcgaaaaaa attcgtatgc cagacatttt tatccattgg
    10681 aaatgtcaaa cattgtggac actaggcagc tttctgcttt ttgatagcca aaggaaccaa
    10741 ttaaatttac aattaggcta tctggaaatg aaaaaacgat taggctatta ctatgggaac
    10801 tggtatacat ttcattctgt taaacctgca agaacatact ggcgaggatt tttgtcttac
    10861 ttaacaaaag aaattcagtt agaattatct tttttcaaaa gcataaaatt ttggcaaaaa
    10921 ctgcgtaatt tatataagaa cagggtcgta ccagagacat gcggattggc gatgctagaa
    10981 cttttagcaa aaaaacattt tttactgccc aatgaaatct atcaagtaga catgatgatt
    11041 caaatgatct gccaacaaga tataaaaagt atgccggatg atttattggc acaaattggt
    11101 caattaagta ctgaagaatg gcgaaggtat cgaaaatacc agcaaaaaat acaaaatgag
    11161 cgcacgaaaa ctgtttattt tgattatctg ctacaaggaa aacgcaagga tcgtttgcag
    11221 catgaattgc ttaagcaacc agttgacaca ttactgatcc tatatttata ttatctcaag
    11281 gaggaacaac catggcacaa gaattttcat atgaaatcgt agaagaaatc gctatattgt
    11341 cagaaaacaa caaggggtgg cgtaaagaac tgaacctagt cagctggaat ggtcgcccgc
    11401 caaaattcga tcttcgagat tgggcgccag accatgaaaa aatggggaaa ggcctgacat
    11461 tgacgaacga agagtttgaa caactgcaaa aagcaataga aaatatgtaa tgaggaagga
    11521 ccctgtcgaa tgaaaagtat gactggtttt ggcaaagcca tccgcgaaac aaaagcgtat
    11581 cagttagata ttgaaataaa aagtgtcaac cagcgttttc tagatgttca aatacgaagt
    11641 cctaaaatcc tgaactttgt tgaaaacgat tttcgtcagc ttattaaaca gcagttaagc
    11701 cgcggtcgaa tcgaagtatt tgtgaatcta gcttatattg gcaaaaacga aaaacagatc
    11761 gtcatcaact gggaattgat tgattcactt atcactcaat tgacagacgg aattcaacaa
    11821 cgatacggta gtcaaaatca agtgtcaatt agtcggatgg cggaacgctt cgtgctggac
    11881 gaaaacttcg taacggtaga agaaaaaccg gaagagagca tggaagaatt ggcacagtta
    11941 gctctagata cattgaaaag cgcacttgat tctattgagg cgagtcgaga aaaagaaggt
    12001 caagctttag ctttagtgtt gaaaaagaat actgaagaat tcaaagaagt actaaaaaaa
    12061 ctcgatagtt ttgtagaaat ctatgaaaag gaatttcaaa taaggtatca aaaaaagctg
    12121 gaggaatttc ttggagcgaa agtcgatgag caacggttgt tgacggagct agccatcttg
    12181 ttggaacgcg gagatattca cgaggaactg gatcgtctgg cgatccatgt acaaaaacta
    12241 gatgaactta tcgaagcaca atgtcctgta ggtcgggaat tagatttcct gatccaagaa
    12301 atgaatcggg aaatcaacac gatcggttcc aaatcaaatg caatcgaaat taaaaatcaa
    12361 gtcgtccagt ctaagacgat cttagaaaaa atcagagaac aagtacaaaa tattgaatga
    12421 cgaaagaggt agagataatg aaatattatg acgtaacgtt ccacgaatta agtggaaaaa
    12481 ctgtggttaa acgggatatc ccttctgaaa aaaacggctt tgacgtatgg aaagatgcct
    12541 gtgcagacta taatgagaac gaactgttta ttttgatcaa cgacggtgct tacgtgacga
    12601 tgaatcggaa atttattgtt cgaattgaca cagaagaggt agaagatccg acagaaaaag
    12661 cacgttctcg aaaagatgag atcatgggtg tagtgaatac tttgtccaat atgggctttt
    12721 agcaaggatg ctgaacaaag aaatacaata aataaacaga attggatttc ttcgcttagc
    12781 ttctttgctc taggaataca tttaaaggtt gtgacaaaat tcgtgtcaca atcttttttt
    12841 attttgaata agtgatgttc aaaaaccatt ttctcggtat taagccaaat ttatgcaaaa
    12901 atgtgagaaa ctattttcac aaaatttttc ttcatactcg aaactaatcg gcttcttcac
    12961 gacctcttat acacggcgtt caaaagcttg catctgcgat aataacccca accaaacgaa
    13021 aaacatgaga agctgtttat gtttggctgt tcttattact tgatgcagga cagctttttt
    13081 acgacctctt ggtacagggt agctttttta ccaatttcta gctataatta aagataacct
    13141 tgaaagttgc agaaaaaaag tgcaaaataa acgataagta caaaaagact agttgaaagg
    13201 acgtcgtcat gtcagagcgt ggattattaa tcgtgttgtc tggtccatcc ggtgtaggaa
    13261 aaggcacagt acgaaaagca atttttgaga gtgaggataa tgattttcag tattcgatct
    13321 caatgactac ccgtaaaatg cgtgaagggg aggtcgaagg cgttgattat ttcttccgaa
    13381 gcaaagaaga gtttgaatca atgatcgaag ctggagagat gctggaatac gcagaatatg
    13441 tcgggaatta ttatggaact cctttgtcat atgtcaacaa gacattggat gaaggaaaag
    13501 atgttttctt ggagattgaa gtccaaggag caaaacaagt caaagaaaaa gtgcccgatg
    13561 gtgtattcat ctttttgact ccgccagatt tagcggaatt aaaatcacgg atcgtaggcc
    13621 gaggaacaga tgcagatgaa gtaatcgaaa aacgaatgaa agttgcaaaa gaagaaatcg
    13681 aaatgatggc tctttacgac tatgctgttg taaatgatga agtgccgcta gcagttgaac
    13741 gaatcaaaaa cattattgcc agtgaacatt ttcgagtaga tcgcgtaatc ggtaaatata
    13801 ttaaaatgtt aaaggagatg tagtcttatg atgttaaaac cttcaattga ctcactatta
    13861 gatcgtgtaa attcaaaata ttctctagtg attttagcaa gtaaacgcgc gcatgaattg
    13921 gatgcaagtg cacagcctac tctagattct tttgattcag taaaaagcgt cggtcaagct
    13981 ttagaagaaa tcgatgcagg aaatgtagtc aatgatccac atcctgaatt aaaacgtgaa
    14041 cgtttgaaga tggaagaaga ggaacgtcaa gcacaaaaag agcgtgaaca acacgaattg
    14101 gaatcacgta tccgtgaaga gcaaaaaatg taataaagaa aaatagagcg aacatctaaa
    14161 aagaaaagca aacccagctg tttccttttc aaacattcgc cacttattgt ctggtagaaa
    14221 aggctgtgtc tgaacttttt gacacagttc ttttttttct tatctgctgt taattttttg
    14281 ttaggatttg gcagaagata agacaaatcc atcgatctct agtaaaatag gcaaggaact
    14341 ttttgtcgct tgataagaaa agacaaaaaa aggaggaaac gaaccatgac attaacagct
    14401 gaagtcattg tggacgtacc tactatgcag accgatcagc cattcactta tttggttccc
    14461 tctgaaatgg aaacagcttt gcaagtcggg atgcgagtag aagtcccttt tggtaatggc
    14521 aatcgtcatg tccaaggatt tgttatggct atccaaaaat cagaagaatc agctaatcca
    14581 tctttgaagg cagttattcg tttgctagac cttgctccgg tggtcaatga agaattactt
    14641 tcattagctg attatatgaa aaaaattact tacgcattca aaatcacttg tctacaaacg
    14701 atgcttccga gtgtgatgaa agcagaatat gataaattga tctatccttt ggcagacaca
    14761 ccagaagttc aggtattgtt tgatcagcga gaagtgattt cctggaagga agcagaagaa
    14821 gaaggcagcc tttctcaatt gattcgctgg cgccaagagc aattagtaga tatcaaatat
    14881 gaagtccata cacgaaataa agtaaaaacg attcgtttgg tccgttcttt gttaactgaa
    14941 aagcaaatcg aagaagaatg ggcaaaatta cggcaaaatg ccaagaaaca aaaagaactt
    15001 cttttgtgtt tatctgaact gagccaagaa gaaccaattg cttatttcaa aaataaagga
    15061 atcagtactg ctgtcttgaa tcaaggaaaa gaaaagggct ggctggagtt tatagaatca
    15121 gaacgttatc gcgaccctta taaagaccga gtatttgacc aaacgacggc attggaactg
    15181 aatgcagaac aaaaaaatgc tgtcgaacaa atcatcacag caggtcagca gcaaaaagat
    15241 caggtctttt tgctggaggg aatcactggc agcgggaaaa cagaagtata tcttcaggca
    15301 atcgctgatg ttttgtcaga aaataaaacg gctattatgt tagtgcctga aatcgcattg
    15361 actccgcaaa tggtcgagcg attcaaagga cgcttcggag aatcagttgc ggtcttacat
    15421 agcggacttt cccaaggtga aaaatatgat gaatggcgca agattgagcg ggaagaagcg
    15481 caagtagtag taggcgcacg atctgctatt tttgccccgc tgaaaaatat cgggctgatc
    15541 attatcgatg aagaacatga atcaagttac aaacaagatg agacaccgcg ttatcatgcg
    15601 agagatctag caatttggcg cagtaaatat catcattgcc caatagttct aggaagcgcg
    15661 acgccctctt tagaatcaag agcacgagct caaaaaggcg tttatcagtt attgcagtta
    15721 aaccaccgag ccaaagcagc tgcacagctt ccagctattg agatcgtaga tatgcgtgaa
    15781 gagtttcaga atcatcggac ctctactttt tctgctaatt tgcaggaaaa aatacaaaat
    15841 cgattagaca aaaaagaaca aactgtccta ctgctcaatc gtcgaggata ttcgtctttt
    15901 gtgatgtgta gggactgtgg atttgttctt ccatgtccta attgcgatat ctctcttaca
    15961 ctgcatatgg atacacgttc aatgcgctgc cattattgcg gacatgaaga acctattccc
    16021 aatcgctgtc ctaattgtgg cggcaataaa atccgctact atggaacagg tactcaaaaa
    16081 gtagaagagg aactccgaga actttatccg caagctagga ttttgcgtat ggatgtagat
    16141 accactcgaa gaaaaggggc acacgaacag attttacaaa aattcggtgc aaaagaagca
    16201 gatatattat tagggacaca aatgattgct aaaggtttgg actttccaga agtgacatta
    16261 gttggggtgt tgaatgctga tacctccctg aatttaccgg attttcgttc tagtgagcat
    16321 acattccaat tgcttaccca agtttcagga cgagcagggc gagcggaaaa agcaggcgaa
    16381 gtcgtgatcc aaacattcaa tcctcaacat tacgccatag aattggctaa aaaacaaaat
    16441 tatgagcagt tttatcagca ggaaatgcat gtacggcatc gtggaggcta tcctccttat
    16501 tactttactg tgaaaataac cgccagccat cctgaagaac aagtagcggc aaagaaaatc
    16561 tttcagattg ccaatcagct aaaagaagtg ttaagccctc aaagcttgct attagggcca
    16621 acgcccagca tgattttgag agtgaaaaac cgatattatt atcaactaat catcaaatat
    16681 aaacatgaac cgaatcttcc tcatgttttg gatgaaattt tgaatggaag ccagaaagaa
    16741 cagcgacaag gtctttttgt ggcaattgac aacgaaccat taaactttat ttaagaaagg
    16801 gccaaattat gcgctatcct atattgatcc accctaatga taaattaaaa agaactgctc
    16861 agccaatcga tgtaatcacg gacgagacaa tcgctttatt agacaattta tatgagacaa
    16921 tgattgcgaa tgacggaata gggatcgctg ctcctcaagt aggacaaaac aagagaattg
    16981 ctgtgatcga agtagatgaa ggcgagaaat ttgaactgat taacccagaa atcatcgaag
    17041 caaaaggtga aagccttgac gtagaaggtt gtctcagtat tccgcatgtc tatggaacgg
    17101 tcaagcgagc agatgaagtg accgtgcgtt attatgatcg agacggagaa gagatagaag
    17161 tgacggcttt tggctattta gcccgtgcct tccagcatga aatcgatcac ttagacggta
    17221 tcttgttcat tgagaaaatg atacagcaga tcccggaaga agaacttgaa gaatatatgg
    17281 aggaacatct tgatgactaa gctagtattt atgggcacac ctgctttttc tgtgccgatt
    17341 cttgaagggc tgatagaatc aggttacgag atccaggcag tggtaacaca gcccgaccgt
    17401 ccagttgggc ggaaacgagt aattacgccg acaccggtaa aaaaagcggc tctgaagcac
    17461 ggtatccgtg tgctgcagcc agagaaaatc tccggttcgc cagaaatgga agaaatcatt
    17521 gaattggtgc ctgatgtgat cgtcactgcg gcatttggcc aattcttgcc tgaaaagtta
    17581 ttgcaagtac caaaattagg ggcaatcaat gtccatgctt ctttacttcc taaatatcgg
    17641 ggaggtgcgc ctgttcatta tgccatcatg aatggggaga aagaaactgg tgtaacgatc
    17701 atggaaatga tcaaaaaaat ggatgcaggc ggaatctatg cgcaagaaag cataccaatc
    17761 actaaacaag atgatgtagg gacgatgttt gaaaagctta gccttttagg aagaaagcta
    17821 ttgctagaaa cattgcccaa tattttggat ggacaaaaac cagtacctca agacgagtca
    17881 gaagcaactt tttcgccaaa catcacacgg gaacaagaag caatcgattg gcataaaact
    17941 gcagaagaaa ttgacaacca agtacgcgga atgcgtcctt ggccaattgc attcacgacc
    18001 tatgagggca tacgctggaa attattatcg gttgaagtat tggcagaaac tacagacaaa
    18061 gcacctggta cgatcatccg gaaagacaaa aaaaatctat ggattgcttg cggtaaacag
    18121 accgtcttag ccatcaatga attacagcca gctggtaaag gaaaacaggc cgtacatgat
    18181 ttccttaacg gaagcggtca gcaagttcag attggtcagc aggtgaaata atgaaaaaaa
    18241 tccctaaaaa aatgaaaaaa tcagtacgat atatggcatt actgacatta gagcgagtga
    18301 acaagggtgg cgcctactcg aatcttttgt tgaatgagat gatcaaacaa agcgagctaa
    18361 atcaaaaaga tgtgggttta tttacagaat tgatctatgg tacgattagc catcagcgac
    18421 tattggaatt ttacgttgca ccattgatcg ctaaagcaaa aaaagtggat gactgggtca
    18481 agatgcttct ttatctttct gtataccaat tagaattttt agataaagtc cctgcacatg
    18541 ctattttaaa cgaggcggtc gaaatcgcaa aagtaaaagg aaatcctggg acagggaaat
    18601 ttgtaaatgg tgtcttgagg aattatcagc gtcaaggagc accagatctg caaggcattg
    18661 ctgatcctat cgaaagactt tctgtcaaaa tcagcctgcc tctatggatg acgaaacgac
    18721 tggtggatca aatcggatac gaagaaactg aaaagctcgg tttatcgcta tatcagccta
    18781 gccatgctag tgcccgagta gatacacgcc gactttccag agatcaggcc atcactgttt
    18841 tgcaagaaga agagatcaca gctgaaaaaa gccaagtcag tccatacggt atagtagcga
    18901 aaaaagggca tttagcttct agctcattgt tccacgatgg cgtcatgacg atccaagatg
    18961 aaagttcgat gcttgtagcg ccgtctatgc aaatcgagcc tgaacatcat gtcttagatg
    19021 cctgtgcagc acccggaggt aagacgacgc atattgcttc ttttttagat ccgtcatgcg
    19081 gtggagaagt ggtcgcttta gatgtacacg aacacaaagt caaactcatt gaagataatg
    19141 cagcacgtct aggtgtctct tctgctgtct acgctcaaaa gatggatgct agagaagtca
    19201 gtaaacagtt cgaagcagag cagtttgatc ggattttagt agacgcgcct tgttcaggtc
    19261 ttgggctgct tcggcgaaaa ccagatattc gttataaaaa acagccggat gaattagcga
    19321 atttaccaaa aatccaattg gaaattttag aaagctgcgc accaacttta aagtcttccg
    19381 gtattctgac ttatagcact tgtacgatct taaaagaaga aaaccaagac gttgtttctg
    19441 ctttcttgga aagacaccct gattttgaga ggattgatgt tttgaccaat cctatgctac
    19501 aatcatcaat cgaagagaaa atgttgactt tgtatccgca tcagttttac acagatggat
    19561 ttttcatttg ttgtttgcgc aaaaagtaat gaggtgaaaa aatgcagatt gaatatcaat
    19621 cagatgtagg aagacgacgt aacacaaatc aagactatgc cagtgtattt accagccgtt
    19681 caggtctcaa gcttgctgta ttagcagacg ggatgggtgg tcatcgtgcc ggagacattg
    19741 ccagtcagat ggcggtaacg aatctaggcg aagcttggga aaaacaagaa gctgttgacg
    19801 atgaaaaaat cgcccaatgg ttcattcaat cgattcaaga agaaaatatg ctgatttatc
    19861 agcgtggaca agaacagccg gaatataacg gtatgggaac aacaatcgta gcggccgctc
    19921 tgtcagatga ctggttcacg attgccaatg ttggggatag cagagcttat ttgattcgtg
    19981 aagataaaat catgcaattg acggaggatc attctttagt caatgaacta gtcaaatcag
    20041 gagaaatcag cgaagaaatg gcagtgaacc atccaaggaa aaatatttta acacgttctg
    20101 tcggcatgcc tggaacagtc gaagtagatg tttctactca tctttggcaa ctaaaagatc
    20161 gactgctgtt atgttcagat ggcttgacga acatgctgtc tgaagaagaa ataggaacga
    20221 tcatcaatca agaaggccca ttgtctcata aagtaactga gctgattgat cgtgccaatg
    20281 aagcaggcgg agcggataat atcaccgttc ttcttatcga atataaggag gacagtgtat
    20341 gatcgagttg ggcaaaaaat taaatggccg ttatcaaatc actggaaata tcggtagcgg
    20401 tggtatggca aatgtatttt tggcgcacga tttaatcttg gatcgtgatg tagcagttaa
    20461 agtcttacga tttgactttc aaaatgacca gactgcgatt cgacgatttc agagagaagc
    20521 tttagcagca acagaactcg ttcatccaaa cattgttagt gtttacgatg ttggtgaaga
    20581 agacaacatg cagtatctgg tgatggaata cgtcaaagga atggacttga aacgatatat
    20641 ccaaacacat tatcctgttc cttatgaaac agcagtaaat attatgcagc agatcctttc
    20701 tgctatttct ttagctcata accatcaaat catccaccga gatttgaaac cccaaaacgt
    20761 actgatcgat aatgaaggtg ttgtaaaaat cacggatttc gggattgcta tcgctttatc
    20821 tgaaacatcg atcacgcaga cgaatacgat gctaggatct gttcattatc tgtcccctga
    20881 gcaggccaga ggaagtatgg caacgaagca gtcagatatt tatgctttag ggatcatcct
    20941 ttatgagatg ttgacgggta gcgtaccgtt tgatggtgaa tctgctgtaa caattgcttt
    21001 aaaacatttt caagatgatc ttccatctat aaaagcactt gatccaaatg tgccacaagc
    21061 actggaaaac gtgattttac gggcaacggc taaagaacct gcagaccgtt ataagtcagc
    21121 cgaggaaatg tctgatgatc tttctacagt attatcacca gctcgtgcaa acgaagaaaa
    21181 atggcagcct catgtgatgg ataatgaaac aaaagttatc acacccctta cggaagatgc
    21241 accaatgcct gattcattca aatctatgcc tctgccaaaa gacaagccag aaaacgaaac
    21301 cttaccagag gaggaagaag ttctgcctga tcctaaaaag aaaggacaga aaaaatggtg
    21361 gctgatccta ttagctattc ttgctgtttt aggaatcgga acagctgtat tctttgcttc
    21421 tggcgggcga ggagaagttg ttgttcctga tgtttctgat ctttctgaag cttctgctag
    21481 agaaacactc gcaaaagcga aattagaagt agcaacaaaa acagaagaaa ttgcagatga
    21541 caaaatcgaa gaaggtaatg tagtgaagac agacccggca gcgggaacga cagtcaagca
    21601 aaaacgcgaa gtcactctgt atattagtag cggcacgaaa aaaatcaaat tagatgatta
    21661 cagtggtaaa agttatgaag atgccgaagc aagtttgaaa gaattagggt tttctgctga
    21721 cttgattcaa aaagaagagg aattcagttc agaggtagaa agcggaatga tcatttcgca
    21781 atctccatca gaagatacag aagtcgatcc taaaacagac acgattactt tcacagtcag
    21841 caaaggacca gaaacagtga ctttgagtag tcatgtagga gaaaactacg aaactgttcg
    21901 taattggctt gttacacaag gattcaatat caataatatt agtgctactt atgactatag
    21961 cgacactgta ccatcaggtt cgatcatcag tcaggatcca gcctcgggtg aagttgtagc
    22021 agaagaaact tacatcaatt ttgtcgtaag ccaaggaaca gagcctaaat tgaatgatat
    22081 ctccggctat acaaaaagcg aagcacaaag ttatttagct agtgtcggtg cagaatatat
    22141 cgggcatgag acttccgaat attcaaacac ggtggataaa gataaagtga ttcgtacagt
    22201 accaggggca ggaacgacaa ttaccaaagg aatggttgtg aacgtaattt actctaaagg
    22261 accagatcca gcgtcttcta gttctgaaaa agactcttca gacagttcta gcagctcaag
    22321 tagctccagt agcacaagca actcttcaaa agaagaatct tccagctcca caagttcatc
    22381 agctagcacc tcagaaagtt caaaataagt gctgaataaa atatatcaga agggaatgcg
    22441 acattgattt gttgcattct cttttttgtg tttctatctc ttttgatcaa ttttcaagcc
    22501 atatactttg agaagtttgc atggaataac acctactttc ctcttcgtat ggtaaaataa
    22561 acaaaaacgg aggtggcact tatcaaagga caaatcagaa aagcaatcag cggtttttac
    22621 tatgtatacg caaacggaga gacctatcaa acaagaggaa gaggaaattt ccggaatcgg
    22681 aaaatcacgc cgcttgtcgg agatcaggtg atctttgaga gtgaaaatca aacagacggc
    22741 tatcttctgg aaatcttgcc gagaaaaaat caattagttc gcccaccagt cacaaatgtt
    22801 gatcttggaa tcattgtcac aagtatcgta gagcctgcat tttcttataa tctacttgac
    22861 cgctttctag ttaccttgga atacgagtcg atcgaaccga ttattttttt gacaaagacc
    22921 gacttgtcag acgacccaga aaaaatcgaa gagatcaagc aaacctatac taaaataggc
    22981 tatccggtga ttgtacccaa tcatacgggt gatacgacag aactgatccg ttacttccca
    23041 gaacggctat cggtctttat gggtcagtct ggagcaggaa aatcgacgct actgaatcaa
    23101 atatcaccgg aactgaattt agcaacagga gagatttctg atgcacttgg aagaggacgg
    23161 catacaacac gtcatgtgga actgattcct atctatggcg ggttagtagc agatacacca
    23221 ggatttagct cgattgactt tttgacaatt gaagcaggag aactgccgag acaatttcct
    23281 gaatttgtag aagcttctgt ccattgcaaa tttagagagt gcatgcatgc taaagaacct
    23341 ggctgcgaag taaaacatag agtagaaaca ggtggtatcg cacagacacg ttacgacaat
    23401 tatttgcaat tcttacaaga aatcgaaaaa agaaaaccaa tgtatgcaaa aaaagaaggt
    23461 aaacgaaaat aggaggaaac aagaatgaag atagcaccat ccattttgag tgcagatttt
    23521 gccaatttac aaagagacat tgagttagtg gaaaaaggag gcgcagatta catccacgta
    23581 gatgtaatgg atggccgatt tgtcccaaat atcacattag ggccaaatat tgttcaggca
    23641 atccgtcctg ttactaaatt accgttagac gtccatctga tgatcgttga tccagaaaac
    23701 tacattccag catttgcaaa agctggagca gatatcatta ctgtccacgt cgaagcaact
    23761 cctcatattc atcgggcact tcaaatgaca aaaagcttag gagttaaagc tggagtagtt
    23821 atcaatccag gaacaccagt ctccatgatc aaacatgttt tgcctttggc tgatcaagta
    23881 ctagtcatga ctgtcaatcc tgggttcggc ggacaaagct ttattgaaga aacagtagaa
    23941 aaaattacgg aactcgctga tctacgtaga gagaacaatt ggacttatgc aatcgaagta
    24001 gatggcggaa tcgttccaga aacagcaaaa atctgtaaag atgcaggggc ggatgtcttc
    24061 gttgcaggat catatatcta tggagccgaa aatccagtcg gacaaatcga acgattaaaa
    24121 gaggcattag actaaatgaa aattttatta gctgctggag gtaatccgca aaactggccg
    24181 cagtctctgc tgtccaaata cgatgcctat gtcgggatag accgtggtgc gctgtacctg
    24241 cttcaagcag gctacccagt agatctggca gtgggtgatt ttgattcatt gtcgaacgaa
    24301 gagcagaagg aagtatttgc aaaagccaaa gagtataccc gttcccctgc agaaaaagac
    24361 gatacagata cacagttagg tatcttaaaa acatttgaac gttacccgca agcggaagtc
    24421 actttgattg gagctacggg cggaagattg gatcatcttt tagccaatct ctggttaggt
    24481 atggaacctc gcttcaaacc ctttgcccat cagcttatta tcagagattg tcaaaattat
    24541 ttgtcttatt atttgcctgg agagcatata gtaagacaga taccagatat gaagtattta
    24601 gcttattgct gcttgactcc cgtctcgaat cttacattaa aaggcagcaa gtatgtattg
    24661 gaaaataaag aagtagaata tccgacatct tatgcaagta acgaatttct ttcagatacc
    24721 gcagaatttt ctttttcaga tggtatgatt gcagttattc aaagcaaaga ttgatccata
    24781 aacaaataaa aaaatttgtc tttcaggcta aaaatgacag aatctattat ttgctaaaaa
    24841 aaagcttaag aggagcaact taccttttgt ttcacggcat ttagtgataa aattgaacac
    24901 aagagtctaa ttaattcgag gtgtctttgt gaagaagttc aatcctaata aaaatattat
    24961 tattaccttg gttattgtta tcatcgtggt gacgatacta agtgtgaccg ctgcgcagcg
    25021 agcgaatgaa ggaaagccca atttcttcca atccatcgtt aatgacagtg ttgcaatggt
    25081 tgataaaaca atctctgccc cagtacggtg ggtacgaaat ggagtagatt ctgtccataa
    25141 tcttttttca acttatgccg aaaatgagcg gctaaaagaa aaaatcgata gctatgatga
    25201 cgtttcacag caaaacaaga attacgagag agaaatcgaa gcgttgaaaa aagaacttga
    25261 gttgaatcag acgctgacta gttacgaaaa agtaacggca aatgtcatta ctcgttcgcc
    25321 tgacagttgg caggacatgc tgatcgtaga caagggttct aaagacggta tcgaagcaaa
    25381 tatggcagtc atgtcgcaaa aaggattgat tggccgagtg atcgaggtca atacggcttc
    25441 gtctaaaatc gaattactgt catcctctaa tgaaagctcc aatcattttc cagtacgggt
    25501 atcttcggct aatggcgaag cgtttggttt gcttaaaaac tatgatgaaa agctccatgc
    25561 cttagtggtg acccaattaa ctggtgatac ggatatcaaa gaaggggatg ttgtccagac
    25621 atccggtctt ggagggaatt ctccagctaa cttgccgatc ggtacggtta ttaaaacgaa
    25681 accagatagt tatgggctgg atcgggaagt ttatgtgaaa ccttatgcag aaatgtatga
    25741 cgtgtcagtt gtgacgattg tccaacggct agcggaggat tagtccatgt tgaagaaaga
    25801 aacaatgaag tactatctgc caatcgtttt gttctttttg atgttgatag atggtcattt
    25861 aacaagaatg ctaggggagt ggtcgaaagg cacctatatg tcaaatgccc actttctgat
    25921 attggcatta ttatgttgca gtatggcgtt tgaaaaacgt tatttactga ttaccacgat
    25981 tgttctcggg gctatctatg acgcttacta tattggcgtt atcggtatct atgcagtagc
    26041 tctcccttta attgtatggt tgatgtatgt aatgaaagac gttatccatg tcaacatctt
    26101 tactgaattt ttcagtatga tcatctttgt cacgggttat gaattgttta cgatggtggt
    26161 ccagttgatt tttaaattag cagtagtaaa taacacgtat tttattacaa ggtttttagg
    26221 acctacactg ctgttgaaca tgattatatt tgtattattc atttttccct ttaagaaatt
    26281 attcagtgat gaataatttc tttttttatt tttaggtcga aagcgaaaaa gaatcgttgt
    26341 tatcttaaga aatgctagac ttaacgcggc tttttcatga tcattttctg aaacgaacag
    26401 ttttgtcatt tttaaaagtt gttttgaaac atttttgtaa cataactatt atatcgttga
    26461 cacactgcta tggtacaatt tttatatcgt taagaaagac ttaaatatat ttagatattt
    26521 attaaattaa gccagtcgga ggaatgaaga gtgaaaaaga gtttaatatc agcagtaatg
    26581 gtatgttcaa tgaccttgac tgccgtagca tcaccgatcg ctgccgcggc ggacgatttt
    26641 gattctcaga tacaacagca agatcaaaag attgcagact tgaaaaatca acaagcagat
    26701 gcacaatcac aaatcgatgc cttagaaagc caagtttctg aaatcaacac acaagcacaa
    26761 gacctattag caaaacaaga tacattgcgt caagaatctg cacaattagt aaaagacatt
    26821 gcagatttac aagaacgtat tgaaaaacgt gaagatacaa tccaaaaaca agcacgcgaa
    26881 gcacaagtaa gcaacacaag ctcaaactac atcgatgctg tattgaatgc tgattcatta
    26941 gcagatgcta ttggacgcgt tcaagcaatg acaacgatgg taaaagcaaa caacgacttg
    27001 atggaacaac aaaaacaaga taaaaaagct gtagaagaca aaaaagctga aaacgatgct
    27061 aaactaaaag aattagctga aaaccaagct gctttggaat ctcaaaaagg cgatctttta
    27121 tctaaacaag ctgatttgaa cgtactgaaa acttcattag ctgctgaaca agcaactgct
    27181 gaagacaaga aagctgactt gaaccgtcaa aaagcagaag ctgaagcaga gcaagctcgt
    27241 attcgtgaac aacaacgtct agcagaacaa gctcgtcaac aagctgcaca agaaaaagct
    27301 gaaaaagaag cacgcgaaca agcagaggct gaagcacaag ctactcaagc ttcatctact
    27361 gcacaaagct cagcaacaga agaatcatca gctacacaat cttcaatgac tgaagaatca
    27421 tcatcagcta cacaatcttc agcaacagaa gaatcaacaa cacctgaatc ttcaacagaa
    27481 gaatcaacag cgcctgaatc ttcagcaaca gaagaatcaa caacagcgcc tgaatcttca
    27541 gcaacagaag aatcaacaac agtgcctgaa tcttcagcaa cagaagaatc aacaacagtg
    27601 cctgaatctt caacaacaga agaatcaaca acacctgcgc ctacaacacc atcaacagat
    27661 caaagtgttg atacaggaaa cggcacagga agtagtactc cggctccaac gccaacacca
    27721 acacctgaac aaccaaaacc tgtaacacct gctccagctc cttctggaag tgtgaatggt
    27781 gcagcaatcg tagcagaagc ttacaaatat attggtactc catatgtttg gggcggtaaa
    27841 gatccaagtg gctttgactg ctcagggttc acacgctatg tttacatgca agtaactggt
    27901 cgtgacatcg gtggttggac agttcctcaa gaatcagctg gaacaaaaat ttcagtttct
    27961 caagcaaaag ctggagactt gttattctgg gggtcacaag gcggaactta ccacgtagcg
    28021 attgctttag gcggaggaca atatatccat gctcctcaac caggcgaaag cgtaaaagtt
    28081 ggttcagttc aatggtttgc ccctgacttt gctgtcagca tgtaagaatg attaatcaaa
    28141 aagagttagc ccattctgga ctaactcttt ttttattgtg ataaaataga aatcagataa
    28201 attcttctgt caattccatg aacgtttgga tatcttcagt caccatgtcg atacctgctt
    28261 gccaaaaatc tggttgggta agatcgacat tcaaatgttt cttcgcaaga tcttccgtcg
    28321 tcattgaggc agtatcgcgt agtaaatgga tatattcttc ttcaaaattt gctccctttt
    28381 ggttggcgta agcatagatg cccaaactaa agaggtagcc aaatgtgtat gggaagttat
    28441 aaaacggtac gtcatcaata aagaaatgca attttgcagc ccagaaatat ggatggtaag
    28501 tagctaaagc tccttgatag ccttcttttt gcgcctcaac catcatctct gtgatcttat
    28561 cttctgagac taatccttct tgccgtgctt catagaatcg attttcaaaa atgaatcggg
    28621 aatggatatt catgaacata gctaaagcat tttgcagttt tgtatctagc aaattgattt
    28681 tttcttcttt tgtttttgcg acttttaatg tagcatcggc tacgatcagt tctgcgaatg
    28741 tacttgctgt ttctgcgaca ttcatggcat attcgcgatt taaagaaggt aaatcccaca
    28801 tcgtgctgct atggaaagca tgtcctagtt catgagcaag tgtcgcaacc tcattaacag
    28861 aattactgta ggtcataaag atccttgatt cttttgtttc tggtaactca gtacaataac
    28921 caccaggacg tttcccagga cgatcttctg cttcgatcca gctcttttca aaagcagatt
    28981 gggcaaattt agccattttt ggactgaatt tgttaaagtt ttcgataata aatgctgctg
    29041 cttcgtcaaa actaaaggtt ttttctttca aatcgcctaa aataataggt gcatcttgat
    29101 cttgccattc cattttttct ttaccgaaaa gattagcttt tctagtcaag taattaacaa
    29161 ggggttgttt gtttttctgg attgtatccc acatgacaga caatgtttct ttcttcaacc
    29221 gattgtactc taatggtttt tgtagaaaat ctgtgacacc atgaagttca tagtcagaaa
    29281 gtctaaaacc atctaaatga ttcaaagtat ccgtgaagag gggtgccttt tctttccagg
    29341 cttcttccca tgctgcgaaa agtgtttctc tgacttttgg atctggatcg cccatcatct
    29401 tattgaaggc ttgtccagca gataattcga ctacttgtcc atcttgttcg aagggaatcg
    29461 aaatgctggc tacaatcgta tcataatgac tgctccaagc atttagacca tctaaagaaa
    29521 gcgtatttat aatgttttct tcagcttctg ataataattg tgagccatca cgacgaatct
    29581 cgtttaaacg aaaagcaatt gtttcaaacg aagattgaga aagcatctga ttccaatcat
    29641 tgtcagatat ttctgcaaat ttctttgaca atacagtttc ggctgattgc agacgaggca
    29701 gcattgcgta aagcttgcct gataagacct ttgcatctga atcattgaca ttggcagaaa
    29761 gcaatgcagt aatatagctg ctgcattggg taaaaccatt cgtaatcgtt tcttgcaatt
    29821 gaagaatagc gtttaactgc tctgcattgt cagaactagg tgaccatttt gttacgcggt
    29881 gataatactc gttcatttgg tcttccaatt gcttcatccg ttggttcaaa gcatcagaat
    29941 gactgcctcc ggaaaaaata gaatctagat tccaatttaa tgaataactc atgaaaaa
//
LOCUS       DARDHA010000017        29166 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000017 DARDHA010000000
VERSION     DARDHA010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.017"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            25..1134
                     /gene="pdhA"
                     /locus_tag="SDA10_000648"
     CDS             25..1134
                     /gene="pdhA"
                     /locus_tag="SDA10_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEG4239578.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            1137..2114
                     /locus_tag="SDA10_000649"
     CDS             1137..2114
                     /locus_tag="SDA10_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEG4239579.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            2139..3782
                     /locus_tag="SDA10_000650"
     CDS             2139..3782
                     /locus_tag="SDA10_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEG4239580.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            3790..5196
                     /gene="lpdA"
                     /locus_tag="SDA10_000651"
     CDS             3790..5196
                     /gene="lpdA"
                     /locus_tag="SDA10_000651"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEG4239581.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            5358..5633
                     /locus_tag="SDA10_000652"
     CDS             5358..5633
                     /locus_tag="SDA10_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEG4239582.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            5639..6406
                     /locus_tag="SDA10_000653"
     CDS             5639..6406
                     /locus_tag="SDA10_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEG4239583.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            6574..8403
                     /gene="typA"
                     /locus_tag="SDA10_000654"
     CDS             6574..8403
                     /gene="typA"
                     /locus_tag="SDA10_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEG4239584.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            8664..10436
                     /gene="recQ"
                     /locus_tag="SDA10_000655"
     CDS             8664..10436
                     /gene="recQ"
                     /locus_tag="SDA10_000655"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEG4239585.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            10550..10852
                     /locus_tag="SDA10_000656"
     CDS             10550..10852
                     /locus_tag="SDA10_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HEG4239586.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            10855..12018
                     /locus_tag="SDA10_000657"
     CDS             10855..12018
                     /locus_tag="SDA10_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEG4239587.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            12044..15472
                     /locus_tag="SDA10_000658"
     CDS             12044..15472
                     /locus_tag="SDA10_000658"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEG4239588.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            15493..16617
                     /locus_tag="SDA10_000659"
     CDS             15493..16617
                     /locus_tag="SDA10_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239589.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            16607..17044
                     /locus_tag="SDA10_000660"
     CDS             16607..17044
                     /locus_tag="SDA10_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG4239590.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            17028..17366
                     /locus_tag="SDA10_000661"
     CDS             17028..17366
                     /locus_tag="SDA10_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEG4239591.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            17485..18045
                     /gene="rsmD"
                     /locus_tag="SDA10_000662"
     CDS             17485..18045
                     /gene="rsmD"
                     /locus_tag="SDA10_000662"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEG4239592.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            18042..18533
                     /gene="coaD"
                     /locus_tag="SDA10_000663"
     CDS             18042..18533
                     /gene="coaD"
                     /locus_tag="SDA10_000663"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEG4239593.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            18523..19566
                     /locus_tag="SDA10_000664"
     CDS             18523..19566
                     /locus_tag="SDA10_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEG4239594.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            19725..20453
                     /locus_tag="SDA10_000665"
     CDS             19725..20453
                     /locus_tag="SDA10_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEG4239595.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            20569..20814
                     /locus_tag="SDA10_000666"
     CDS             20569..20814
                     /locus_tag="SDA10_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG4239596.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            20811..21029
                     /locus_tag="SDA10_000667"
     CDS             20811..21029
                     /locus_tag="SDA10_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEG4239597.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            21165..21704
                     /locus_tag="SDA10_000668"
     CDS             21165..21704
                     /locus_tag="SDA10_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HEG4239598.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            21697..23970
                     /locus_tag="SDA10_000669"
     CDS             21697..23970
                     /locus_tag="SDA10_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEG4239599.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            24008..25054
                     /gene="holA"
                     /locus_tag="SDA10_000670"
     CDS             24008..25054
                     /gene="holA"
                     /locus_tag="SDA10_000670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEG4239600.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(25143..25397)
                     /locus_tag="SDA10_000671"
     CDS             complement(25143..25397)
                     /locus_tag="SDA10_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEG4239601.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(25797..26066)
                     /locus_tag="SDA10_000672"
     CDS             complement(25797..26066)
                     /locus_tag="SDA10_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239602.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(26536..26991)
                     /locus_tag="SDA10_000673"
     CDS             complement(26536..26991)
                     /locus_tag="SDA10_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEG4239603.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(27129..29057)
                     /locus_tag="SDA10_000674"
     CDS             complement(27129..29057)
                     /locus_tag="SDA10_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEG4239604.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT         9709 a         5113 c         6198 g         8146 t
ORIGIN      
        1 agatgagaga ggaatgtgtg acaaatggca aacaaaaaag caccgatcga cttccaagca
       61 ctgctagatg aagtcaatgc agatttccca gtatatcaag cacttgatca agatggaaac
      121 gtagttaaca aagatttagt acctgattta tccgatgatg aactcgttga attaatgact
      181 catatggtat ggtctcgtgt attagaccaa cgttcaacag cgctgaaccg ccaaggacgc
      241 ctaggctttt ttgcaccaac tgctggacaa gaggcaagtc agctggctag tgcttacgca
      301 tttgataaag aagatgttct tcttccaggt tatcgggatg tacctcaact agtaaaacat
      361 ggtttacctt tatctcaagc ttttttatgg tctcgaggac atgctgcagg taattcttat
      421 ccggaagaat tgaaagctct accaccacaa attatcatcg gtgcacaata tgtacaagca
      481 gccggtgttg cattaggttt gaagaaacga aacaagaaaa atgtcgtctt cacttatact
      541 ggtgatggcg gttcatcaca aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac
      601 catgcaaatg ccgtatttta tatccaaaac aacgggtatg cgatctctac acctcgtgaa
      661 aaacaaactg cagcaaaaac acttgcacaa aaagctgtcg cagctgggat tcctggtatt
      721 caagtcgatg gaatggatcc gcttgcagta tatactgtat ctaaaatggc acgggaatgg
      781 gcgatcagtg gaaatggacc agtcctaatc gaaacactga cttatcgtta tggaccacat
      841 acactatctg gtgacgatcc aacccgctac cgtacaaaag acacagatga cgattggcag
      901 aaaaaagatc cattgatccg cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa
      961 gaaaaagaag aacaagtcat cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa
     1021 gccgacaaag taccaaaaca aaaggtttca gatttcttga aaaacatgtt cgaagtatct
     1081 ccacaatcaa tcaaagaaca aattgcaatt tatgaagcga aggagtcgaa ataatcatgg
     1141 cacaaaaaac aatgatccaa gcaatcacag atgccttagc acttgaacta gaaaatgacg
     1201 agaatgtcgt tgtttttggt gaagatgttg ggaaaaacgg aggagttttc cgggcaactg
     1261 aaggattgca ggaaaagttc ggagaagacc gagtattcga tactccttta gctgaatctg
     1321 gaatcgcagg attgagtttc ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc
     1381 aattctttgg ttttattttc gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa
     1441 gatatcgaat gagcggcact agaaatctac ccatcacaat tcgttcgcca tttggtggcg
     1501 gtgttcatac accagaattg cattcggata atttagaagg tttgatcgct cagtctcctg
     1561 gtattcgagt agtgattcct tccaaccctt atgatgcaaa aggactattg atttcttcta
     1621 ttagaagtaa cgatccggtt gttttcctag aacacatgaa actatatcgc tcattccgag
     1681 aagaagtgcc ggatgaagct tacgaagttc ctttggataa agcagcagtt actcgtgagg
     1741 gaacagatgt atctattatc acatacggag caatggtgcg tgaagcaatc aaagcagcag
     1801 acaatcttgc aaaagaaaat atatctgttg aaatcattga tcttcgtaca gttgcgccat
     1861 tagatgtaga aacgatcatt caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag
     1921 aagcacaacg acaagcgggc gttgccgcgc aagtagtttc tgagatctca gaacgcgcta
     1981 tcctttcatt agaagcccca atcggacgag tttctgcacc tgatacagtc ttcccatttg
     2041 gacaagcaga aaatgtctgg ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa
     2101 tcgcggaatt ttaagaaaag aagggacgaa acaaagtcat ggcttatcaa tttaaattgc
     2161 cggatatcgg tgaaggaatc gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag
     2221 atacgatcaa tgaagatgat acattactag aagttcaaaa tgacaaatca gtagaagaaa
     2281 tcccgtcacc tgttacagga acggttaaaa atgtgattgt tccagaaggt accgtagcaa
     2341 atgttgggga tgtattagta gaaatcgatg caccaggaca cgaagacaat gaaggggaca
     2401 gtggtgtagc cgcagaatcc caaactccag ctaaaccggc tgctgaaccc actgttgata
     2461 cagaatcagc tggatcgtca agcgaaggag tcttccaatt taagttaccg gatatcggtg
     2521 aaggaatcgc agaaggtgaa atcgtcaaat ggttcgtcaa accaggagat acgatcaatg
     2581 aagatgatac attactagaa gttcaaaatg acaaatcagt agaagaaatt ccgtcacctg
     2641 tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat gttggggatg
     2701 tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt gcgccatcag
     2761 ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt gaagctgccg
     2821 atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt gaaaaagatg
     2881 tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa gaggacatcg
     2941 aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg ccagaagcgg
     3001 cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca gcaaaaccat
     3061 tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca cgtaaagcta
     3121 tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg catgatgaag
     3181 tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct gctgcaaatg
     3241 gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact gttaagaaat
     3301 acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat aaacattact
     3361 acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat gtgaaagatg
     3421 cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca aaattagctc
     3481 atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc agcaatatcg
     3541 gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta gctattttag
     3601 gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc gttgtcggtc
     3661 gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca actgcgcaac
     3721 aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg atggaaggat
     3781 gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt aattggtgcg
     3841 ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa agtagcaatc
     3901 attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc ttcaaaagca
     3961 ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt tggagtaact
     4021 agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa caaagttgtc
     4081 catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga aacaatcgaa
     4141 ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga ttcagctcaa
     4201 acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga gattccagga
     4261 tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa agaagtacct
     4321 aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg agcgtacgca
     4381 aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc aacttacgaa
     4441 aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac agtcgtaacg
     4501 aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa atatgcagtg
     4561 gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg tcgtcgtccg
     4621 aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg aggattgatc
     4681 acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg tgatatcgtt
     4741 ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc tgaagcaatc
     4801 tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt cactgatcct
     4861 gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat cgaagcaaaa
     4921 gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa aacagaagga
     4981 ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca aatcggtgga
     5041 gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg aatgaatgct
     5101 gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt catggatgct
     5161 tcggaattag cattaggttt accaatccat atttgatttg aataatacag gcgaaacgca
     5221 actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt atgtaaactt
     5281 aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa gaggaatatg
     5341 aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat tggacaacag
     5401 acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat gaaaaaggga
     5461 taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc aaatccattg
     5521 gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct ttatatcgta
     5581 cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg tgagataaat
     5641 ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga aaatcaaatc
     5701 ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag taacaaatgt
     5761 cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc cagaggcgaa
     5821 aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa gagtctttat
     5881 catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt tttgtattat
     5941 gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg tgatgagaaa
     6001 tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc cattgcctag
     6061 accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc ttttcggtca
     6121 taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta tgtacggatg
     6181 cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata tttcaaacct
     6241 tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta tggtaacaag
     6301 cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag cagggacttc
     6361 ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa aaggtagaaa
     6421 tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa atgaaaacat
     6481 tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa tttgccctgt
     6541 cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga tatccgtaac
     6601 gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga actattaaaa
     6661 caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga ctcaaatgcg
     6721 ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga ctataaaggt
     6781 attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga agtagaacgg
     6841 atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg aacaatgcct
     6901 caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat cgttgtcgta
     6961 aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt tcttgaacta
     7021 ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta tgcatctgca
     7081 ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac catggcacct
     7141 atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga cgaaccatta
     7201 caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg aatcggacgt
     7261 gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt agacggaaca
     7321 gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg tctagagatt
     7381 caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat ctttgtcggt
     7441 gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat tgacgaacca
     7501 acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga aggtaaattc
     7561 gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga tgtatcctta
     7621 cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg agaattgcat
     7681 ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt atctcgtcca
     7741 gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg cgtgcaaatc
     7801 gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg taaaggtgaa
     7861 atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt aacacctgcc
     7921 cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta cggaatcatg
     7981 aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg acgtcaccaa
     8041 ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat gagtatcgaa
     8101 gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat gattatcggg
     8161 gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca aatgacaaac
     8221 gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca attgacgttg
     8281 gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc agaaagcatt
     8341 cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa aaagaaaaaa
     8401 taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct ggtttctttt
     8461 tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa ttcagaaaat
     8521 acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt tttgtttcat
     8581 aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat cgaagtaaaa
     8641 tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt ggttatgatg
     8701 agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa aatgtattag
     8761 gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct ttactattag
     8821 atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag gtggacgcgg
     8881 cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt tacgaaacag
     8941 ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc gctcctgaac
     9001 gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt atgatcgcta
     9061 ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt tatttacaga
     9121 tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg acagctacag
     9181 cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa aacaaccata
     9241 ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat caaaaaaaag
     9301 aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc atttatgcag
     9361 caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat ttttccgtgg
     9421 gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa gcctttttat
     9481 acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata aataaaagca
     9541 atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac tatcaggaag
     9601 ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc tctcctcaag
     9661 atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg caaaagcaaa
     9721 aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc tgtcttcagc
     9781 aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt ggtaactgtt
     9841 tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt tcttgcttaa
     9901 aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca ggatcgaaag
     9961 aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg atgaaaaacc
    10021 aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc tatcttctga
    10081 ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa gtattgaaag
    10141 gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg gatgaagaaa
    10201 ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca gaagcaggcg
    10261 ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga atacgtccat
    10321 caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt gataaatatg
    10381 gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat aagtagaaag
    10441 aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt gctccttatg
    10501 aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga tggattcttt
    10561 gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca aacgattaat
    10621 ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag aagtggtaga
    10681 tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag gagtaatcgg
    10741 aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc agttatatag
    10801 tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat agacttgcct
    10861 aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta tttaatttta
    10921 tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt agaaaacgga
    10981 caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag cttgattgct
    11041 atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt gatcatggca
    11101 gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa ggagatcaat
    11161 ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga atacctaaaa
    11221 atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag tgtacaaaaa
    11281 gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac tgcattagtc
    11341 gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt ggttctgctt
    11401 ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg cttttgtcta
    11461 agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc cggacgttta
    11521 cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga attaaataaa
    11581 gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt tggcagggga
    11641 ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga ttttatttat
    11701 gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc gttgctattc
    11761 tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt caattcgctt
    11821 ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct tggaggaatt
    11881 actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg aggctccagt
    11941 ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga tgaaaaacgc
    12001 aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa aagttttagt
    12061 tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat taggaattaa
    12121 aactgttgga atctatgcag aagaagatga atattccgta caccgtttca aagcggatga
    12181 agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata ttgaagggat
    12241 catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg gtcttttgtc
    12301 ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg ttggacccaa
    12361 actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag ctatcgaagc
    12421 ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg atgccttgga
    12481 gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg gcggcggacg
    12541 cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac gtgcgaaaag
    12601 cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca tcgctaatcc
    12661 taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc atctttttga
    12721 aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc cttgtgtctc
    12781 tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga tgaaatatgt
    12841 cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt tttactttat
    12901 cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta cagatattga
    12961 tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag aaatcggatt
    13021 gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa tcactacaga
    13081 agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc gttcacctgg
    13141 tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag tgacaccgta
    13201 ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa cagccattca
    13261 gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa acattccatt
    13321 tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa caaccttcat
    13381 tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa acaaaacgat
    13441 gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg gcgaaaaacc
    13501 attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt acgtaacggc
    13561 taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca aaggacagga
    13621 aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc tagcaactcg
    13681 tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc ttcctgaact
    13741 cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct ttttgaatga
    13801 agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt tgctgcaaat
    13861 gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg tattggtgga
    13921 atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg atagtttgaa
    13981 ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta aaatcgcaga
    14041 ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat acaatgttca
    14101 gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca ttgcaatcaa
    14161 agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg aattgaaaga
    14221 aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg gaatcatcac
    14281 ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga gtgcaatgag
    14341 tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca atggtccacg
    14401 gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg aagatgtgcg
    14461 catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag tatatatgca
    14521 tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg tcggattagg
    14581 tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt ttggcgatat
    14641 cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta tggtccaaaa
    14701 tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc ctgaatctgt
    14761 tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta aaaaattgca
    14821 aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt tggctgaatc
    14881 agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg aaccaaaaca
    14941 agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc aaaaagcata
    15001 taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag gtatacgttt
    15061 gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc gtttagatga
    15121 aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga atggacaaag
    15181 acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc gattgaaagc
    15241 tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt tggatgtatt
    15301 agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg aagcgatgaa
    15361 aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat atgtgtcttc
    15421 aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat aataagcgaa
    15481 aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt agttattgtg
    15541 gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga aaactctgat
    15601 caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc aactggtttt
    15661 tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca agcctatgat
    15721 cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc tagtcatctc
    15781 acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact gcaaacaggg
    15841 cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac gaaaatcact
    15901 actatctata ctaatttcac ttttgagtac catgatacag aagttggaat cgaactaatg
    15961 gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt tgctatttta
    16021 ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa agatagtctt
    16081 ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca agcagataaa
    16141 tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac tttattaaat
    16201 atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt tcaaaaccac
    16261 agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc agaggaacgt
    16321 atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt ctcactgacg
    16381 aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag tgggctattt
    16441 attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga tccttacaac
    16501 catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc taaagaaaat
    16561 atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga tcgttaacga
    16621 agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa aaaagagtaa
    16681 aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa aagtttgcca
    16741 gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg catatggtga
    16801 gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa gacagcttga
    16861 tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa gtctgttgga
    16921 tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag atgcaggtag
    16981 cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg caattagaca
    17041 atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac agcttaaaac
    17101 aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga atgaaatatg
    17161 tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc cgtaaattac
    17221 attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat tttgccgaaa
    17281 aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag ctaaatacag
    17341 aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa ataaaaaata
    17401 acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat actagtacca
    17461 gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg cgggcgccgt
    17521 ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa agaatccata
    17581 ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt tgcaggaagc
    17641 ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg cgtagacaaa
    17701 aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga accagagaaa
    17761 tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc ggagaaacaa
    17821 caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat cgatcaaatc
    17881 cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg cgaaacggat
    17941 aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa acagatttat
    18001 ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt gcattattcc
    18061 caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt gcggctaaat
    18121 tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt ttgtttcccc
    18181 cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat gtaaaagtca
    18241 tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca gatgcgttga
    18301 tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct cagatgaatc
    18361 atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa tatagtcacg
    18421 tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc agctacttac
    18481 cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc taaacaatgg
    18541 ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt agtagttccc
    18601 ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact aattacggtc
    18661 aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg tatcaggcaa
    18721 gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt gatcagtgaa
    18781 gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca gtattatatg
    18841 gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa gccttataaa
    18901 atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt ctttggcaag
    18961 ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag tacagaagca
    19021 tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag ttatttgcat
    19081 aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga taaaaaggca
    19141 ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga tgttcgtatt
    19201 aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga aatctatgaa
    19261 caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg aacgatcaac
    19321 agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag tgcggataaa
    19381 gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt taagaaagaa
    19441 tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa attgaattcc
    19501 tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct agagaaaatg
    19561 aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa cccctctttt
    19621 tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt aattagatgt
    19681 tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa ggagtaatga
    19741 atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc agcaagaaaa
    19801 tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc ttttgtatcg
    19861 caagtggttt tttattatca aatttgctat tttatagaga aacagaagag actgctgcaa
    19921 aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa ggaaacgatg
    19981 tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt gtgaaggagc
    20041 ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag cgtgcaggtg
    20101 gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata gaagatcagc
    20161 aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat cagttggcat
    20221 cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat atcaatacag
    20281 ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg caagaaatca
    20341 ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag gagatttctg
    20401 gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa tagaatgatt
    20461 caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat aatccaattc
    20521 taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt gattgttcgc
    20581 aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat atctgggatt
    20641 catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga acgctttgcc
    20701 aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac gaatgatcta
    20761 gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg atgatagttc
    20821 agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca gcagtcgatg
    20881 agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct gttctcacag
    20941 aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc ttgaacaaat
    21001 ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg gcagtgaagg
    21061 gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt gtttaagcaa
    21121 tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta gaaaaatctt
    21181 caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat gaacgcattc
    21241 cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt acgtgtacac
    21301 gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc ggctataatg
    21361 gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg gatggacact
    21421 gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa cttggtatcc
    21481 cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt acaaaggctt
    21541 tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat gatccttatg
    21601 cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta gactcacagc
    21661 atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt agaaaggcat
    21721 gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt gttgtattac
    21781 actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt ttcgctaaaa
    21841 gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc catttcttat
    21901 aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat tactgtattt
    21961 acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt atcagagaat
    22021 aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa aacatggctt
    22081 aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc tccagcaaaa
    22141 aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag aatttctgga
    22201 cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg aaactggctt
    22261 cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca aaaaacaagt
    22321 gtatatatta attctttatt atggggctat aaagatgcag gattcagaga accagaagaa
    22381 atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca catccaattt
    22441 tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca cgtcagtatt
    22501 attccgttga gtctactaac tatctgttat gtttctattg caggaagttc tatttctgtt
    22561 ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa taagcattat
    22621 tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga tcctcgattt
    22681 ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat cctgatttct
    22741 tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat gatcacgatg
    22801 ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt tacattactg
    22861 attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac actcttcaat
    22921 cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt atttgagcga
    22981 ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct tgtgctatct
    23041 gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca acctgtgaaa
    23101 cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt tttacgcttt
    23161 tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt gcaaagtcct
    23221 tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga aagagaaaag
    23281 tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata tctaaaaggt
    23341 caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga tcatgtggga
    23401 gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg ttggggcgct
    23461 ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg tactgagatc
    23521 attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt attgactcct
    23581 tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga atataacaat
    23641 aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt gcttttgatt
    23701 tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg tacgtcaagt
    23761 aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg cggcgtaaat
    23821 aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa tcagataaaa
    23881 acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt ttttcatcct
    23941 catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg taggataatt
    24001 tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac gtaaagaaaa
    24061 acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc aagttcggac
    24121 agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt taagttttga
    24181 catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc cgtttttcgg
    24241 agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa aagtaaacaa
    24301 tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat tagaatcgac
    24361 tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag taacgaaaca
    24421 attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag aagtacgtca
    24481 atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg caattgattt
    24541 attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac agaaattggt
    24601 cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat tagttcctaa
    24661 aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga agacggaaca
    24721 agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca aaattaatgc
    24781 gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa agattggtta
    24841 tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa aacttgccat
    24901 gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc aattagttga
    24961 gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc aactattcgt
    25021 tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc aaagcatgcg
    25081 ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg aagccgattc
    25141 ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt gtttttgtgg
    25201 attagacctt ttgattcagc catatcaata gctttaacag cttctttata taacgcatct
    25261 acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt acgcatagcg
    25321 cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg tttgattgca
    25381 gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc gtgttaccgc
    25441 ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg aacatgacaa
    25501 gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg atagtacaag
    25561 ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca aactagattt
    25621 ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt attatataac
    25681 aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt tgttctttgt
    25741 ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag taaaggctat
    25801 tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat tttttttatg
    25861 accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt atttaggggc
    25921 aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat cacacggtcc
    25981 tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac gtacgtatca
    26041 attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag taacctgaaa
    26101 acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat tctttctttt
    26161 gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa aaagagcatc
    26221 gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt gattaaaaaa
    26281 agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca aaaagcagta
    26341 tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag aatcaaaagt
    26401 accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc gaacattgta
    26461 tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa tctcctaaaa
    26521 tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt aagtgatacc
    26581 agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta aaatcgtgcc
    26641 gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac ccttggaaga
    26701 taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg atttaaaggc
    26761 ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa tagccgctgt
    26821 gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa aggaacggga
    26881 ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac tttgattttt
    26941 agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca tgaaatatat
    27001 tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa gtaggaattc
    27061 atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat cataaaaaaa
    27121 cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga cttacatttt
    27181 tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt cagaataacc
    27241 aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa ttgcaggaag
    27301 ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg tttgatgatc
    27361 cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc gcttttcatt
    27421 aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc gacacctaaa
    27481 gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc atttacttgg
    27541 taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca gcttgcatag
    27601 taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg gtcgccggtt
    27661 atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg ggtatttgct
    27721 tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga aagataaatc
    27781 acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt gctgattatc
    27841 atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa ataggtagtt
    27901 tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc aggtattgtg
    27961 caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg ttccatagca
    28021 acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt tacaactggt
    28081 tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc agaaagcttt
    28141 tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga aagagcagct
    28201 agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac taataatacc
    28261 attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg gagaaggatc
    28321 attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg ttcgataaat
    28381 gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat cttagcaaag
    28441 acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg attcagcgtt
    28501 cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc gcttaatgca
    28561 gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac agctgctcct
    28621 ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc ttgcatttgt
    28681 ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc agtaatttct
    28741 ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa gatgaatgtc
    28801 aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc taaagccatc
    28861 aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc cgatgtttgt
    28921 ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt aagagagaat
    28981 cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc tgcaaaagct
    29041 tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag cataataaaa
    29101 atgtaattga taataataac agctaaatga gaacgataat ggttatcatt tggtgattaa
    29161 gcttga
//
LOCUS       DARDHA010000018        27668 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000018 DARDHA010000000
VERSION     DARDHA010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.018"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(220..1164)
                     /locus_tag="SDA10_000675"
     CDS             complement(220..1164)
                     /locus_tag="SDA10_000675"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEG4239605.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1460..2404)
                     /locus_tag="SDA10_000676"
     CDS             complement(1460..2404)
                     /locus_tag="SDA10_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4239606.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2625..3839
                     /locus_tag="SDA10_000677"
     CDS             2625..3839
                     /locus_tag="SDA10_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4239607.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3861..5876
                     /locus_tag="SDA10_000678"
     CDS             3861..5876
                     /locus_tag="SDA10_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEG4239608.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5959..7332)
                     /locus_tag="SDA10_000679"
     CDS             complement(5959..7332)
                     /locus_tag="SDA10_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEG4239609.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7358..7642)
                     /locus_tag="SDA10_000680"
     CDS             complement(7358..7642)
                     /locus_tag="SDA10_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4239610.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7644..9659)
                     /locus_tag="SDA10_000681"
     CDS             complement(7644..9659)
                     /locus_tag="SDA10_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG4239611.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9838..10140)
                     /locus_tag="SDA10_000682"
     CDS             complement(9838..10140)
                     /locus_tag="SDA10_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HEG4239612.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEVALAGTNSGETEDKEESLAGTN
                     SGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10229..11260)
                     /locus_tag="SDA10_000683"
     CDS             complement(10229..11260)
                     /locus_tag="SDA10_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG4239613.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11467..12342
                     /locus_tag="SDA10_000684"
     CDS             11467..12342
                     /locus_tag="SDA10_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG4239614.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12494..13336)
                     /locus_tag="SDA10_000685"
     CDS             complement(12494..13336)
                     /locus_tag="SDA10_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG4239615.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13428..15305)
                     /locus_tag="SDA10_000686"
     CDS             complement(13428..15305)
                     /locus_tag="SDA10_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG4239616.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15302..16723)
                     /locus_tag="SDA10_000687"
     CDS             complement(15302..16723)
                     /locus_tag="SDA10_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG4239617.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16726..20115)
                     /locus_tag="SDA10_000688"
     CDS             complement(16726..20115)
                     /locus_tag="SDA10_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEG4239618.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20372..21763
                     /locus_tag="SDA10_000689"
     CDS             20372..21763
                     /locus_tag="SDA10_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4239619.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21836..22483)
                     /locus_tag="SDA10_000690"
     CDS             complement(21836..22483)
                     /locus_tag="SDA10_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEG4239620.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22491..23177)
                     /locus_tag="SDA10_000691"
     CDS             complement(22491..23177)
                     /locus_tag="SDA10_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4239621.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23183..24256)
                     /locus_tag="SDA10_000692"
     CDS             complement(23183..24256)
                     /locus_tag="SDA10_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4239622.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24478..25152
                     /locus_tag="SDA10_000693"
     CDS             24478..25152
                     /locus_tag="SDA10_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4239623.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25157..26497
                     /locus_tag="SDA10_000694"
     CDS             25157..26497
                     /locus_tag="SDA10_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG4239624.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26646..27632)
                     /locus_tag="SDA10_000695"
     CDS             complement(26646..27632)
                     /locus_tag="SDA10_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEG4239625.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8061 a         5237 c         5027 g         9343 t
ORIGIN      
        1 ggatttttta attaaccaca gataaacatt gataacgatt cgcttattgt aatcaacaga
       61 ttaaatttca tatgttcata tgtacggata cattttcgga ccgattaatt ttaaccattc
      121 tgtttatttg cttacctatt agatatcaat aaaaaactgc ttaacagcta tttcagctgt
      181 taagcagttt taggattttt agttcatatt cattgtttct tataatacag tgttcaatac
      241 ttctcttaaa gcacttgttg atttgctgaa cattgctttt tcttcttcgt tgattgataa
      301 ttcaacaact tcacgaacac cattttcgtc aacgattgat ggcacacctg taaagatatc
      361 tttttcgtcg tattcaccag ttaaataagc agatacaggc aatacagctt gttcgttatt
      421 caagatagct tttacgatac gtgcagtact cataccaata ccatagtaag ttgctttttt
      481 acgatcaatg atctcataag cagcattacg gactttatca gcaatcacat ctaattcatc
      541 ttgggcaata cgatggtctt tttctacaat ttcaaacact ggtttaccac cgacagttgt
      601 atgtgaccat gcagcaactt ctgaatcacc atgttcacct aagatatagc cgtggacgct
      661 gcgtgggtca acttttaatt tcaatgcgat ttctttacgg aaacgagttg tatccaatgt
      721 tgttccagta ccgataacac gtgatgttgg cagaccagat tcgttccaag cgatatatgt
      781 caaaatatct actgggtttg aagcaacaac gataataccg tcgaatcctg atcccatgat
      841 ttcttttacg atttggcgca tgattgacgc atttgtttta actaaatcta aacgagattg
      901 accaggtttt tggttgatac cagctgtgat aacaacgata tcagcatctt tacattcttc
      961 atagccacct gaccatacag ccacattttc atcgccccaa gccataccat ctaaaaggtc
     1021 caatgcttcc ccttctgctt tttcttgatt cacatcgatc aatactaatt cattagagat
     1081 tccttggttg atcattgcat aagcgatact tgttccaaca aaacctgtac cgacgattac
     1141 tactttacga cttgtttttt tcatgaataa acagctcctt ttcataacta tttgttactt
     1201 tcatgaaaag tatagtcttg tttattcatt ttatcaacat gttaattata atagttgtta
     1261 ttataatttt tgatggaaac gaattcctga atacgcataa cagataaatg acaaatgttt
     1321 acacctatat cccctgataa tacaaccaaa agtttagaac tgaaaataaa tccaaaaaaa
     1381 ttcgctgcaa cgatataata agacagcaat cgcacctaaa acaattttcc ttcatatgct
     1441 ttagtgaaag attttcttct taatgtttat ttctccattc attgggagta catgttgaat
     1501 acttcttgaa taatttgaat aaagaagtcg tttgtttata tccgatacga ttagcgattt
     1561 cttccaacga aaaactcgtt tcttgaatca gttccaacgc aaagtcgaat tttttcttta
     1621 gaaccagttc ttgaaaggaa gcatcaaaat acattttcaa tttattacta agatagaccg
     1681 gattgtagcc aaaatgagct gctgtttctt ttaacgtaca agaagtaaaa tgcttatcga
     1741 tataatctaa tactctaagc atctcatttt ctgcgcatcc atctgctgat gaaaagcttt
     1801 gttgttctaa taaatgagtc aattcgatca gtaatagtga tgtatagaga tttaacgaac
     1861 ggatcttatg actgtatgta tcaaaataaa tcaacagcat attttcaatc aaatgcgtca
     1921 aacgacttgt ttttccgccg cgaaaatgaa tgaattgtgt atgttcccct ttttcttttg
     1981 atgcatttat caaaaaagaa gaaagtagat tatttgtgct tgccaaatga ttcaatatct
     2041 ctgtagataa ggtttcttct tttaataaga tgttgattaa aatatcctct tgccctgtgt
     2101 attcgattct ttgagtagct tctttatcaa tgatcaccac gtcactttct tccatctcta
     2161 tcctttctcc attaataatt tgtaaacatg taccgctata catataattg atttcaacaa
     2221 aatcatgtgt atgctcggga acttccgcat atcgacggtg tttactgata tgaatatttc
     2281 cctgttgaaa tagatcccct cggtggattt ttggcggtgt ctgaagatgg taagctaagt
     2341 ttaaatcatt gatattcatt ccttgttcac gctgcagagt ttcgattgat tcttcgttac
     2401 gcaaaaaaaa tgtctaattc agctagttcc aattgactcc ctcctttttg taagcggttt
     2461 ctttcgctta ttagttcacg ctgtactgct attatatgca aagctatttt tggtttcata
     2521 aagactttct atggttggga catgaacttc ctgtgattcc gctattgaga gaataaccac
     2581 ttcatactat atttttgata agcgcttaca aaaatgaaag gaacatgctt atgaataaaa
     2641 agatgatgaa attttctctg ctgtcagtct ctcttatttt gacttctgct ggtgcagtca
     2701 cgtctgcaat tcccagtatg tcagctactt atgccacgcg ttctttatct gctgttgaat
     2761 tattaacgac tgctccctct tttatggtca ctatttttgt tttgattagt agttttattg
     2821 ctaggaaaat cggacaaaaa caaactgtga tcatcggatt agtgattgca tcgatttgtg
     2881 gtatcctccc cctcttctca caaagttaca gtattgtctt agcttcaaga gctggactag
     2941 gtattggctt tggattgatc aattctttgg cagtcagcat gattgctgcc ttttatcacg
     3001 gagatgaacg agcctctatg attggttttc aaagtgcatt tcaaggattt ggtgcagctt
     3061 taatgacatt tgttgcagga cagctagtac agttcggttg gcaatatacc tttttagtct
     3121 acgccatcag cttacctatt cttgtcttgt ttgttttgtt tgttcctaca cctgaaacaa
     3181 ctgaacttca atcgcatggc acagccccaa caagacaagt aatcaaaacg aatcctaaaa
     3241 tgattagtta tggtattttt ctcttgctgg tcatcgtggt atataatgct gtatcgatta
     3301 aattagccac agtcctgacg acttattctc taggaaacga aactaatgca gcgaatatct
     3361 taacaatcat gcaattggca agtatggctg ccggagtttg ttttggctgg cttcagacaa
     3421 aagcacatcg ctatatgctt acactttctt tactactgat gggaatcggc tttgtattga
     3481 ttgccactgt gataaacctg tatgtgattg ggtttggtgc gatcctaaca ggaatttctt
     3541 tttcgttatt cattccttac ttattcaacc aagtaagtat ccaagcgccc aagtacgcgg
     3601 agagtttcaa tacttcaatc ttattggttg gcgctaattt aggtactttc attgcaccat
     3661 atggtttgct ctttctctct tttgcttctc ttgggagtaa aacacttgct gttttcgtca
     3721 atggcagtat cttgctgttg attagcgcgc ttattagcct tctagtagta cacaggacga
     3781 atagaaagac agtgataaga gtagatgaag aaataaaaga acaattgaca acaaaataaa
     3841 tttgatggag gtcgtgtaaa atgacaaatg aaaaacataa agatagatgg ctttggtatc
     3901 ctggagactt tgagatccgc catggactgc tacaaaattt ccaacgggaa gaacgaggtt
     3961 ttgactggcc agcgtactgg tacatggatg attgccatcg gaacgtcaag tttaagagat
     4021 attactttct tgatcaacct tccatgttta aagtgacgat ccaaggagtt gggtatgtag
     4081 aaattaatgg acaaaaacat ccatgtggaa aatggctgac ttgtccagct ggtaaagcaa
     4141 aaattcgtat ctttgtagga catacatcag ggctaccagc gatgtttatc gagggagacg
     4201 aagtaaaatc agatattggc tggacagcaa gtaattttat tgaagaatat ccagctggct
     4261 ggagtccgct ttatgtcgat atcgcaaaag atccaaatca aatctattat caaaaagaat
     4321 gcgtccatcc agtcactgaa acggagattg gcggaggaat cttattcgat tttgggcgag
     4381 cagtgaatgg ctgtgtagaa ctaatatctc tggatgacca tatcgaatct atcactctat
     4441 gttatggaga gtccgatgta gaagctatgg atatcgatta ttgctactat aagcaaatag
     4501 gaatgacaag aaaaatgaaa gctcgtaaac gcgcttttcg ctatttgttt ataccagagg
     4561 tttctactgg aatggttcaa cttcaagttt ttcatgaatt tttatctctt tcgaaacaag
     4621 ctgctttttc aagcgattct tctttactga atcagatctg gtcagtatca gaagaagcct
     4681 tcaagttatg tagtgatttg ttttttatcg acggtatcaa acgagatcgc tggatttggt
     4741 ctggagatgc gtatcaatca ttcatgatca atcaatatct tttttttgac gaggaaatca
     4801 ataaacgaac gatattagca ttaagaggac aaaatgaaat caagcagcat atgaatacta
     4861 tcgttgacta ttcgatcttg tggttgattg gtatcgaaaa ccactatatg atgacgaaag
     4921 actatgaatt tttacgcatc gtctacccta agatgcaatc attaatgaga tacttgatgg
     4981 agcaaacgaa tgaattaggg tttatttatg ggagagcaaa agattggata tttattgatt
     5041 ggtctgaaat ggataaagaa ggaacgcttg ctgcagaaca agtcttatta ctgaaagcct
     5101 atcattccat gatcacttgc ggagatatct tagctgaaac atatggagag aacgaaaatt
     5161 atcatactca tctctatcaa gaaaaatatc aactattgaa acaaaaccta tttgactact
     5221 tttgggatga agaacttggc gcttttatcg attgctatga atctgggaga agaaatgtga
     5281 cgcgtcatgc gaatattttc gctgttttat tcgattttgc taacaaagac caacagcaat
     5341 cgatcttaaa gaatgtctta ttgaatgaca ctatcacaca gattactact ccatatttca
     5401 aattctatga acaagatgct ttatgcaaac tcggacagac gaatatcgtc taccaaacga
     5461 ttcttgatta ttggggaggg atgttatctc atgatgcggt aactttctgg gaagaatacg
     5521 accctactca aacaggaaat gaaaagtaca gtatgtatgg agatccttat ggaaaaagct
     5581 tatgccatgc atggggagca agcccaatct atcttttagg acgctatttt atcggtttgt
     5641 atccaacaaa gccaggttat gaagaattta tgatacaacc taatctagat tcatttgaaa
     5701 cactgaattg tacgctacct atcaaagatg gaaagatttc gcttgaaaaa acaaaagata
     5761 agatcgtgat tacttctgat agaacaggcg gaacagtgaa aatgaatggt aaaagttatc
     5821 ctcttctagc aaaacagcca cttgtcttaa caagctattc aaaaataaat gcataaaaca
     5881 aaaaactgct tgcgacatat cagaagactt taaatcttct tctgatattg tccaagcaat
     5941 ttttattttg aagtttgctt aattctgttc tttagaaaca atgtttgttc tttttccggc
     6001 aaaaagaaag ctagataata cgacaagtaa aaagaaagcg atgattccaa ctgtcaaggc
     6061 tatttgaccg tattctccta gtttcccaag tacaagtcct ggtactagat aatcagtatc
     6121 ggcaaatgta gaagaagcga ttttaagatc tcctaatact ggaaggataa ataatggcag
     6181 ccaactaatc aataagctat tgaaagcaga acctaatacc gcccctcttc taccgccagt
     6241 cgagttgccg aagacaccag aagcagctcc gcagaagaaa tgtcctacta ccccaggaat
     6301 gataactgta gtttgcaagc ctatcatagc aaacatggat agtgttccta caaagaagct
     6361 aacaaaaaag ccaattaata cagcattagg tgcatatgga aaaacgatag gaacatctaa
     6421 tgctggttta gaatcgggta cgagtttttt agcaatccct tggaatgctg gaacgatttc
     6481 tgccaacacc attcgaaccc cttgaaggac gataacaaac ccagctgtga atgttccagc
     6541 ttggatcagt gcaaagatta cagcgttgtc tccattgctt agttctcttt caacaaatcc
     6601 aggaccagct aacaaagcaa gtgcgacata tacgatcgac atcaagagca tgatcgatac
     6661 agtagaatct cgcaaaaagc tgaaactttt aggaatcttt atgtcctctg tagaacggtc
     6721 tttgtttcca aaaaactttc cgatccatcc actagaagca taaccgatat ttccggtatg
     6781 ccctaacgca acttgattgt ttccagtgat gttcttcata aacggttgac aaagagcagg
     6841 cgtaatagtt aatagtgttc cttcgaagac cccgcctaat aagatcatcc acaaatttgc
     6901 agtaaacccc gcgcttacta aaatgactgc tgttagacaa gaaacataaa gcattgcttg
     6961 acctgtaaga aaaatgaatt tatatcttgt gaatctagca agtaaaatat tcacgatcat
     7021 ccctactagc atgatcaaag cagtaggtgt accgtattgt tgcaaagcaa tcgccactac
     7081 tgcttcattg ctaggaacta ctccttgagt atgcagagct gtttggaaca tcgaagcaaa
     7141 aggggttagt gaattactga ttaaaccagc accgccgatc agtactagga atccgacaaa
     7201 tgttttgata gttccttgga tcacttctga aaatttcttc ttctgaagaa cgagaccaag
     7261 cagtgcaatc aaagaaacga ggatggaggg ctgactgacg attgacacaa acaaattcaa
     7321 caatttatcc attgatagtt cctcccttga atcattttta ttcttttgac aagatcttcg
     7381 ttactttttc ttttacttct tcgctgtcaa tgatcgattg caaaggtacg agcttttcat
     7441 caggtataga agttagcgta tctttcaaag aatgctcaat gaagaaataa tcagccatat
     7501 cgctagatac acttccaaca tccgtatgac ttacttcaaa ctcgtccaca tctgctccaa
     7561 gatctgcaag tgcttgtttg atgttcatct ctaccataaa acttgttcct aatccacttt
     7621 gacatactgc aaaaattttc atatcattcg tctccttttc ttatgatcgc taatatttct
     7681 tcttttgttg atgcgtgtaa taaatcttga agattttgtt tattagaaag tagctttgtt
     7741 aaatttgcca aagcccgaag atgtgcctca ttatcgatag cagctaaaca gatgaacaaa
     7801 ctaatgggat gggctggatt atcaagcaga tccacaggtt gttgaagttt cagcaaagac
     7861 atccctactt cgtttacgcc atcttctgga cgagcatgag gtaatgcgat gtgatcgcca
     7921 atatggataa acgcaccata ctgctccacg cgctcgatca tagctttgat ataactttct
     7981 tctattttat tttctgataa tagcggctgt gcggcaaatg tgattgcttc ttgccagttt
     8041 tttggttgat ccgataattg gatcatttct tctgttaata attcagatag cataggacgt
     8101 tgatcctcct tcttttctaa aagtttgttc attttacgat ttaatacttg atagagcttt
     8161 tcttcgtcta cgccttcttt taattcgata taaggcagta gcgccgacaa caattcttgg
     8221 gcagaaggca ttgaaaatcc tggtatcagc caatctcttt gtacttgatt gatcagctga
     8281 tttttttcca agtcagtcat gattggatgc agaacataga gagacttttt cttatcagta
     8341 agtgtgagag gtacagtaga aaaaataata tcgtaagaag tttcgtcaac ctcgtgtaag
     8401 cggtcagctg aattagtctc tgtaaaaaag atcatcggga ataattgctg aagttcactt
     8461 tttaagatca atgaagagct aatgccactt ggacagacga tcacggctct tatttttcta
     8521 tttctttctt ctgcgtccgc ttttctgatc tctccgccaa atagtatagt gaaaaaacca
     8581 atctcttctt cagggatgct cttccctatt cgtctttcta acggaagtaa tgcttttctt
     8641 gtcatttcgt agatggaagc atattgatgc ttaatttgat cgatcatcac attaggtaaa
     8701 taaaaagaat aggaaatacg aaaataggca gggaccaagt gataaaagac attcatcaat
     8761 aattcacgaa acgaagcaaa ttctacggcc atcaggaatt ccatgcgatg gatgacttct
     8821 gaagaacatt ccagcaaaaa atcaagtgca ggctcctgga catctccttc aacgatcgat
     8881 gctaagcaaa gagagaaaaa tataatctcc gaattttgat gttcggaata gccaaggcca
     8941 gtcaatactt gtcgactaat gtcagtgact gcaagagaag ccaacagttc agcatctttt
     9001 accagttcca tggatgtttt tccaattcga atggttgtca aactcaaata ataagctaat
     9061 tcatccattc gatttggtgc gaatcgaaga tctgatagtg aaatcgtttt atctagcaaa
     9121 tctctaactt tggcgtaata atcaatggaa agggttgcgg ttaaatacgt gagcccaaat
     9181 cttccagcaa gtgactgcat caaacgagca gtgcgatttt ttgcttctct tcttaatatt
     9241 gcttctgacc ctagcagcct aaaaccagtt ttcctagtat acgacagctg acaatcttcc
     9301 acttctaatt catttcttat ctttttgata tctgacagga ctgtcccttt agaaacctga
     9361 agaaaatctt gaaaatgaaa aacagaaaca gacgttgcag tcgaataaac catcaaataa
     9421 agaagatctc ttcgctcttg ttcggtatac acagcttcaa atcgagtaat atccaaaaat
     9481 ttttctgccc agtcgattgc tcggaatttc tttatacgga tacgggtatc atccacatat
     9541 tcggcaaaag gagttaattg tttgatcgcc tcgcttgtat ttacttcgat gatctctgct
     9601 gctttttcta gactgatttc tcttggtgtt ttcaggagat aactaatttt gtagttcaca
     9661 gcgctgttca cctcctactt atattctagt atggtagcgc ttttaaatcg aggggtgttt
     9721 ggtacaactt acagttggat tttttttacg actgtaccga acgatttcta tcgaatggat
     9781 acagaaaaaa agcaggtgac caaatgtctc ctgcttttat gaattgattt cgtccgatca
     9841 atgatgatca tgtttatttt ttgaggtcag ttcttcaaat ttttctttga catcttcaac
     9901 cgctttttcg atatcttttt caaagtgatt gggctcatta ggctttgttt cctcttctac
     9961 agaatttgaa ccagataaag ctgtttcttt attttctgtt tcaccagagt ttgtgccagc
    10021 taatgattct tctttgtctt ctgtttcacc agaattagtt cctgctaaag ccacttcttc
    10081 tgttttaggg gccattgaac ctcttaatcg tttgtgtttt tcaccgtcat aatttgtcat
    10141 gttggttcct cctagtagtt ctatactttt tcatcgtaca ataagacagt ttatgcgtca
    10201 aaaaacaaac ccgattctta caaatatgtc atatttttcc ttctgattct tgaatgcttt
    10261 tgattccttc ttccaaaatt ttcatttgta agattgtttc ttcatctttg atgacttttt
    10321 ctgcgccatt aactaatgtc tcgtaaatcc cttcgtagac acggctgtaa tcaccaacct
    10381 cggaaattac tttttcttca tgataactcc cttgttcatc tatataggtc aatacaccat
    10441 aatgttcggg caaatcgata ccaaagtctt catgatcagg catgtaaaac attttcagat
    10501 gttcttcttg tcgatctttt gtttgtttca cgaacatccc tttttttccg taaacgacga
    10561 aacttggacg ttctttgatg cggaaataac tggattttat cgaaattttc atatttccat
    10621 agaaaaaatc cagatcgaag taatcattca ttcgtccttt ccctaaaagt tgacgtacat
    10681 catagctgat atcatctggt tcgccgaaat aagaaagaac ttgatctact gtatgacaag
    10741 catgtccata taaatagctg ttcgcagtag aaaattcatg cgtattttct ggtacttccg
    10801 gtctgaaata atcataatgc atttctactt ctaaaatatc ccctaatttt ccgctttcga
    10861 ttactttttg gactgtcaag aagtcagaat cgaaccgtct gttttggtaa cattggataa
    10921 atagaccttt ttcttttgct aatgcaaata actcttctgc ttcctttgac gtttctgcaa
    10981 aaggtttctc tactagtaca ttcttaccat gttcgagtgc catttttcca tattcataat
    11041 gaaattgact aggtgtagtg atgacaacta attggatctc aggatcatcc caaagatcag
    11101 acagctgatc agtatattcg atgtcttcga atgttttcca cactggttca gatggggaaa
    11161 aaattttttt gatattgatt ttgttcgtaa gttttcttga aaaaggcaaa tgatagcgat
    11221 ttgtactctt tccattgcca agataagcga ttgtaagcat gagtggtgcc tccttttatt
    11281 gatgttttca gtatatccta gaaaagcgca gaaagtattt ataatagcta ttctggtaaa
    11341 aaagttcaga aaaacacttg ttatcattcg gtttggtgaa attaatgttc aaagtaagga
    11401 taaaaatcaa aaagatagta gcggaaccaa gtgtttatca gtaagataga atttggaggt
    11461 cagatcatgc ttattgaaga aaaactaaca aaacaagagt tgtttaccac gacagagaaa
    11521 cggattgtcg attacatccg taggaatatc gaagctgcag tctacatgac gattgaagaa
    11581 ttagcaaaag ctacgtatac ttcccattca gctatcattc gtttatgcaa aaaaatggga
    11641 ttcagcggat ttaaagaatt ccgtttcgag ctggggcggg aagttcatca attgattgct
    11701 cagttcaatc aaacgattga tcctaatttt cctttttcaa gtgaagatag cactcagcaa
    11761 attgcaaaga aaatggcgga tttatcgatc cagtcaataa aaaaagcaca attgcagatt
    11821 gaacatgaag atttagatca aatcggtaaa gtattgaaca aggcacagcg aatctttctt
    11881 tttgcaaaag gtgattctca aatcacggca aggaaattcc agaataaaat ggtcaagttg
    11941 aataagtttt tgatcatggc tgaagaatac agtgattctt cttggaatgc agccaatttg
    12001 actagcgagg attgcgccat ttttattagc tacagcggtc gcatccatca ttatgaacga
    12061 ataatgacct atttgaagca tgtagaggcc cctaccttgc tgattaccgg aaatcagcat
    12121 tcagaaatgg ccaaacaggc tgatatgtgt cttgttatat cgcaggagga atatgatttt
    12181 gctaaagtag cgactttctc ctctcaaatt gccttcgatt acgtattaaa tacgttattc
    12241 tcagtgatat attctcaaaa ttttgaagag aatatccgca acttgaaaaa caaacaagaa
    12301 ttgatgcaaa aaggccttct taaagaatat cggaaaactt aagaaaagat cctcatttag
    12361 taataagaaa gatagtgttt gataggctct ctttgatatc atcataaaaa caggctgaag
    12421 ttttcttttt caaacctcag cctgtttgtt tacggaacga ttcgaataga tatattatag
    12481 aaatgaggaa tagctaactt ctgtttctta ttttgaacga ttgacgaaaa agatatataa
    12541 gaaggaggaa aatcagtata catgcagcta acaatccata caggaaaaat cgtttgttct
    12601 cggatacttt ctgtacttct ttttgaactt cttttgcatg ataaggaacc cggtgccctc
    12661 tgacaagtaa gcgatgactg ttgaccatat aaggtgtaca agttaggagt gtcacgaaat
    12721 cctgcccttt ttcgattctc agatcttttg tatcagtcgg ttctaccgtc ttgatctgat
    12781 cgacttgata agcgagtgtc cgtccattga tttcgatata gaaagaatca cctaaagtca
    12841 gtttcggtaa gtctgtgaac aactttgctt gaggcagtcc tcgatggctg gaaataacgg
    12901 catgtgtatt ttctcccccc gtaggataag acgttccttc caataatgag gcaccttttt
    12961 ctagcagcac agtattcgtt tcatcaaaaa ttggcaaatg gacattgatc tcagggatcg
    13021 tcaaaatacc gatcgtatgt ttctcaaaat aggttctatc ctttttcgca ggttcttttt
    13081 tcttagtaaa gggatctgca cctggatttc caccttcttt tgctaattgc tggtttttct
    13141 ttgtcatgtt ttcttggatt ttagccatga cttcttcatt ttctttgacc gcttgttgct
    13201 gataatgaga gatgatctgc tgatccagat aattattcag cgcgtcgcta acaaatggat
    13261 aagctagtat ccctattcct gagagaagca agaaaagcat cagcagatct agaagtttcc
    13321 ttttctttgg tttcatttgt gttctccttg tgatgactgt ttgataataa aagaaaaagg
    13381 agcatttgcg taatccgctg ctcctttttc caattcttgc aaaaagacta aatctgactg
    13441 tgcttgcgtc tagcaaagta cagtccagca atcagtagaa ggactactcc tgcaccgata
    13501 tacacataga ttcccttacc gcctgttgaa ggaagtgtac ctttgtgttt atttggtatt
    13561 ttttcaggag aaatcaactg tcctgctgtt acataagatt gttcatcgat agtaaatgct
    13621 acacggtttg ttaatggaac atatttttct ggcgctttcg tttcttccag atagtacgtg
    13681 ccatatttta gacctgtcac atcgattaaa ccgttacttg tggttgtaaa aaccgttgct
    13741 gattctttcg ccgataccca gctgacggct tttgtggatg ggtcgatcga taaatatttc
    13801 gctgtgtcac tatcttgatc acgaacgacg aattctgctc cagcaagtgt ttggtctgat
    13861 gtaacgtcac catctacttt aacaaatcgt ttgcccccag taacgacatc gactgacggt
    13921 ggtgtttgat catttgtatg cccgttatcg acatttgctt ggttagaaaa tcctttggtt
    13981 ggatctgctt tttcgttcaa atgcatatag taaacgaatt tcaatgtacc gccaggagtt
    14041 aatgaaggaa tataattcgg atcaactgaa accgtgaatc cgtctgtttg ctcagtgaca
    14101 gaataattta ctgggtcgat ttctttattt ccatcatata aggcataagc atacgttcct
    14161 gaagaatcat tatcaaatgt taaagcagcg tcatgagtat cgataagttt gaatgttgtg
    14221 tacttgtttt gcgttccttc tttatcagcg atacctaatg ggatattgac agaaatctca
    14281 tattgaattt tttcaccgat tgcgacatct tttccattca attgaggtgt tgttttatca
    14341 actttagacg tatcattttt gatggtcttt tctactggtg tttggctatt tgccaggatc
    14401 tcaaaaggcg tgattgtttg cttttcaatc atttccgcat tatcaggagc ttttacttct
    14461 tctaaattat attttccaac ttctaaatga ttaacgatca actggccttt ttcaatagat
    14521 gcctcggcaa agtcattgtt gccgatgtca taagaatgac cagtaatgaa atgtttggct
    14581 ttggtttgat cagttgtcca agtgtacaat ccatctgtga cactttggat gtattttttg
    14641 acgcttggtg ttccttcttc tttagaaata ataaattctg ctccatttag tgcttcgttt
    14701 tcggcagtac cgatttttgt aactttcaac gttccatcat taccgactgt atttttaggg
    14761 tagagatgga tagtatctag ttcttctgtc ccgtatttat aagagccatc tgcttgtttg
    14821 atcatctcat atacagggaa agctaaaacc atgtttgcgg cagctgacac tccgtctttt
    14881 ggttcttctt tgatcgtgta gactgcatct ttcccatttt gttttttagg taaagataaa
    14941 gtgacatttc catcagcatc tgtcgttcct gaagcaactg gtgtaccagg agtcaaagac
    15001 tggactgctt gttttgctgc atccacggac gctcctttat ctcgttgcgc ataaaattct
    15061 tgagtgacgt tataaactga aaatgaaata tcggctaatc cttggtattg atcgaattcg
    15121 ctcatttctt tcccgctgtt ttggattaaa ggatcgggta aatcagtcat tttcttttta
    15181 tgaaggatga cttgagctgc tgtctcctct gcaaacactt tttttgcgcc gaagctgttt
    15241 gtgagtaatg gtaaaataag gaaaaggact cctaacataa cgtttatttt tttatgattt
    15301 ttcatttttt cactcctgtt ctttttttga taaacaacca gattgcaaag cttgcgaata
    15361 ccataccaac aacgcctaaa agtgtatttt tcgtttcgtt tgtttttgga agaaatggac
    15421 ggctattttc ttttttattg actactttca atatactgtc cgcctcttta tcctcagaaa
    15481 gaaaagttga aatggtaaac ggaatttctg tttcacttaa aatataacca gaaggtgctt
    15541 cgatctcttt taatcgataa cttcctgttt tcaacccgga aattgaaaaa gcgccatttt
    15601 tatcggaaat caggtgaagc gcatctgatt cgttctttgt ccacttgtat ccgttggctg
    15661 tttgctggag gtaatttcct gctttgtttt taataaggaa tactgcgcca gctaatcgtt
    15721 tatctggttt atttgtctcg attttgacaa attgcttact ccctgtatat acagagactt
    15781 tctttgtact gacaccaaaa ccaaaatcca gtgttccgtt gttgttgatt tccttgttcg
    15841 cctgtgctgt actgcttagc tgcatctgat aagaaatggt cagttttttt ccagcatatt
    15901 tttccaacga agggatcgaa aataaaacat caaatccgtg aggtttcttg tgtatgacaa
    15961 agccttctgt cagcttttct ccattcgatg aaatcgttaa actattaggt aaaaacgtta
    16021 atgcttcatc cgcactatct gacaattcga acttttgata gtcaaggata tttacaggaa
    16081 ttgtcgtatg caaggaataa gtgatttttt caccaatcgt aaaatcattt ctaggctctt
    16141 ctaatacctt ctcaaaaggt ggatcagggt agtcgttttc ttcattttta ggatacaaat
    16201 ggatagttga aagtttttga ttgttttgtc catgtacagg aagaacaact accatattct
    16261 ctgccttttc tttgacgact tctggtactt tggattcaat gaaaagatac gctttatctc
    16321 ttttttcttg atctttagcg gccagagaaa aagaagcgat accatcttcg ttgttaagag
    16381 ttgtagttgt tgcttctgcg gtaaacatac cggaagacgc accgtttttt gcgatctctg
    16441 cttgtgcttc ttctaccgtt ttgccctttt cccgtagatg gtaaaaagaa tctgtgacat
    16501 cataaacttg gaatgtgaca ccatttaatc ctcgatacgt ttgtaataaa gctttttctt
    16561 gtccgtcatt tggatgattt ttcggcagtt gcccgtttgg gaaaagcaat ttgtgtaaag
    16621 taatctgaac catttctgtt tctgtttctg ttgctacctg agtaaaagct ggtttaaata
    16681 gtaacaagaa gagacacata ctaagccaac caagtttttt catatttagc tgccccctct
    16741 ttcttttttc aaaaaaagat accaaataga aaacgcacaa ccaatcatcc ctactagata
    16801 gattcctaaa cggccaattc ctcccgtttc aggtaatggt acttttgcct gattcgtgat
    16861 gtctaaagaa atctggttgt ttttggctcc tggtgacaga acagattcat gatcttgtcc
    16921 atccacttga attgacccat cttcgtgtat aactatagta actggctctt ttagaccttg
    16981 gtatccttct ggtgtaaaag tttcggtcag cgtataagtt ccaggtttta aattttcgaa
    17041 tagaaaggta tctgtttctt gtccatcttt tggcgattct aagtccattt ctggtccctg
    17101 cagtctgaat tttgctcctt ttaatgtctg tccgttgtct tcttttttgt gtaccgttag
    17161 atcaaacgct tttagtcgat tttgatgagt gagcgtccat acattatttg tcatctcggt
    17221 attttttccc gtgtaaatga tttttccata tttatcgatt tggaattcat aatttcccgg
    17281 cgaagtatca tatccggcag gaccagctgt ttcaacgact cgatatttac caggtgtgag
    17341 tgaatcaaaa ctggcaagac ctttttcatt cgttgtttgg ctgtcaattg gctgataagt
    17401 accatttgct tcttttttct gtagggaaaa tgctgctcct ttaagattta tctctttgtc
    17461 cgtcccttgc atcgcatact tacgtactgc tacaggaact tcaacaaaag gattttcaat
    17521 agtcaactgg atcgtatcat cggaagtagt gactgttttt ccatcaatgg ttaccgtacc
    17581 atcagaagcg atcttgattt cccaagtagt ctttttgctt aatccatgcc cttctggagc
    17641 ttttgtttct gtcagcgtat aggtcatttc tttttgcaat ttgacatttt ctggaagaga
    17701 ataggttccg tcgccatgat ctgttagtaa agtatcaata gaatctcctg ttaatttgaa
    17761 aacagcgcca ataagatcct tttcacctgt agaggaattt ttcgttattt tcaagtttaa
    17821 cggtgtgaat tgtttagtat ttttccatgt cattgcatct atttgttgag aatcgtatcc
    17881 tggtacaggt aattctttga ttgtttggta actgaatgct tgaccttgat tattgtattg
    17941 aggtagtgat aatatctctt gataactttc tccgctattt gcagataatt tgtcaatgtc
    18001 tgcacgttcc cacgtatttg ttgtatcttt agctggttta atgattcgaa tatatccgtt
    18061 tttccaagct gcagcatttg tcgtatgttc ccgttgaatc tcaaaagtaa cttgatctgg
    18121 acgatcagct agattgttgt caaattcttc ccataacttt ttgatgtgaa gactgactcc
    18181 gggagcttta gcagatggta taccgaattc agctaattca ttggtatcaa tgtttggctg
    18241 gaaagttgtc ctgccgttca tttgatacca gaaatttgga tggaagtcct cattttctgt
    18301 ttggattctc acttggtaat ggatttggat ttcttggtct tttcctaaat agatctgatt
    18361 gctcttgatg gtatttcctt ctatggaaat agatggagtg accgttgtag gacttgtccc
    18421 cacactcttg actgaaagtg taccaggctg ataaatgaat ggttctgcga ttggatcatt
    18481 tatttgtcca ttgcttacag ttgctgagat ctgtacagct tttttggcta ggtattcaga
    18541 gatatctgtt gcatgatcag ctgattcata gtaaagatcg cctttttcat ctgatgaaac
    18601 catttgtcgc atacgagact ctacttctgc ttttgagaga ccagctgccg gatcgctttg
    18661 aagttggatg ccaagaccat gtatttcgat tcctcgttct ttgagtgcca tcgcttctcc
    18721 gatggttgcg ataaacgtac tgtcgattct tctggataga ttgttttggt caggtgcatc
    18781 atagattctt gagattagag aagtatttcc cggccgatct tgcgtattag aaaactgagt
    18841 tccgtaataa ttgctgcttg attgtgcgtg tacccgctgt actttatagg aaaatgttgg
    18901 tacaccatcc gtcagcaaaa cgatcacttt tttatgtcca tttggaacgg atagcatgct
    18961 tcctgcatct cttagtgctt tttgtgtaaa agtaccacca tttgtccgtg aaggtgtaat
    19021 ggattttact tgatttttca ctgaatcaaa tgaacccatc tgtactgcac cgttactgta
    19081 gctatatcct tcgcttgagt agccgacata tcccatattg attttgtctg tgataccgct
    19141 atctgctaaa gtatcgacaa aacgatcgac accaatcttt acttcaccga tccgattatt
    19201 gtcgttcata cttcctgacc agtctacgac caatacgaga tcaagaggcg tgatatcttt
    19261 ttgtacattt ccacgagcat tcaaataaac atcaaacaat ccgggtgtac ttgtttcctt
    19321 tgcatacttt cgtatcgcat agtctgcttg attaccggtt cctccatatt cgatatacga
    19381 atgggtccga tcattaggat ctccattcca agaatttata ccatcccatt gatttgttcc
    19441 gtttttattg ccttgatgat caagaacatt tgtatttcct gtaggttgcc aactggcttt
    19501 aggataagtg ccctgatcat ctgttgtata ttctgtagga atcgtttgat tagatggcac
    19561 actcacagct ggcaaggata atgtaggcaa tgtaaatggg ctgacagaat tttcgttttc
    19621 ttttgatgat tcttcttcta tttcgtctgg ctttggtact gacacatctg tatctttctt
    19681 attttctgtc tcatcaataa tgtcatattc tttattatcg acgatcagct tgtttgcctg
    19741 gctgtggtaa agtgctggat cgattggaaa actcagatcg atagtttgtt tagtgtttga
    19801 tgagatttcc aacgtaattt tcccgctatt tacctgataa gcatagccat ctccttcttt
    19861 tctttccaca tcacttaagt aattttcgac tagaccaatt tctttttcta tcttttcagc
    19921 actggtgtta ttcgcattta gcttgatgtt cagtgctttt tttccagact gatccgaaat
    19981 gacttcagct gaaattgttt ctaaaactgt tggatcatct gtagtgtctg ctattgcaga
    20041 taatggcaca aagtttgata aactcaatat tatcagcatg aaaataactt tcagtttctt
    20101 ccctgtggtc gtcatttttc ccctcctttt tctttttttc tgttttgacg tgaatagtga
    20161 aaattattaa aataaacagg cgttacataa ttttatttat tttaatatga taattattat
    20221 acgaagggcg ctaaagggaa tcacaccaat tttcatacca attggtttca tttatgaagt
    20281 tgattccttc gattaatgag cgtttcattg aaagaaaagg gttgagggag aaaaacatat
    20341 gatcaggttg ttggaaggaa actatagaaa gatggaaaaa atcattcgaa ttctgtccat
    20401 ttctgacaag tggatgacta aaaaagaact tgccaaacga attggtagtt cagaatctac
    20461 atttgtaagg tatatcgaag agatcaaaat ccgctgggga acggatgtaa cgattcaatc
    20521 ttcacaaaaa ttggggtatc gtcttgaaca tttgaatgta ccagcttatt taaatgttct
    20581 gatggaaatg tcgcaaagtt ctacaaacag tcgtttatta aatgagatta ttgtcaatcc
    20641 gggtaaaacg attgagtatt attgtgaaaa aatttccatc agccggtctt cttttacacg
    20701 taagttgaaa caatgcaatc aaattttaga aagctattta ttaaaagtga tcgttgacca
    20761 aggttttcag ttaactagcc ttaaaagtga gctccagttg cgtatctttg ttaccttttt
    20821 ctttttaagt tactacggaa gacgcacgtt gccatttcca atcgaaaaaa agaagatcaa
    20881 agaaattatg gtcaagaata attgtcagat gaatttgttt caggaagaaa gcccatatga
    20941 gcaatctttc tttattatgt attttgttgt gcatctcatg agagaatccc aaggaactcc
    21001 atttgaatgg aaagctggag actcttatgt acagttgata aatatcgata aaaaagactt
    21061 cttaatcttg aaaaatctct taggaaaatt gaagtacagc agctatataa aagtagtgaa
    21121 ctattttgtc aatacgattt tttcagaatt ttcggagaaa gaaaaaaatg aaatcagtga
    21181 gcagatacga aaaaatatgt cctactgttc gttttcatcc gctgattgca aagaaaaaaa
    21241 cgaagaatat gttgttcaat tattaacgca aatttatttt ttctcccgta ttttcccata
    21301 cgatacgact catttgacga tccgtgcaac ttactttgca cagcagatgc aacaccgcca
    21361 gatacgtcta tatgctggat ttcgtgcaga acttcaattt tattccactc tactgaatca
    21421 gaatctgatt tattatttat ctagctatat cttttggatg cttatcggta atcctgaaat
    21481 tcaccattat acttctgata aaaaaatact gattatcagc gacttaagtc ttcgacatag
    21541 tcaatatatt gaagaatata tcagtgatat tcttgctgtg cataaaattc actctgagac
    21601 aactgctatt actgaagatc agctgtcgaa atacaatctt ctcgagtatg atctgatcgt
    21661 tacgaatcaa cccattttga atgcccatgt tcctcatata ttgattgatg atagtgtgtc
    21721 attcgcaaat gaagaagaac tcatccgctt attcgagtta tgaataatca aaaaacagct
    21781 atgtataaac caccctgata aaaatttcag gacaaataca catagctgtt tctttttatt
    21841 gtattccttc agcaatccaa cgagatttat agcgaaagtc ttttaacaac tcttcttttg
    21901 tccaattatt tgcaatgaga tccgtcaaag tgttcattac gagggtcatg gaaaagtata
    21961 ggaaactaac tgcttcttcc gacgattgaa tggagaaatg attgattctt aataattcca
    22021 gccatttttc gttcattgga gaatcagccg atagttctct acgtttggtt gtctgctgat
    22081 catttcgctg ggttaacaag tataaccttt gccaatagat actttcgtga tctaagtcgc
    22141 agcaccactc taaatatttt tcgatcccca aaaagaaatg ttgtttgttt tcgctaatga
    22201 attttaagat atcttgatgg actatgtgag aactgctttt gatcgtatac aaataagcat
    22261 cttccaaatc ttgaaaatat ttataaaaag caccgcgcga catccccatc ttgtcaacga
    22321 tttctgagac tttgacttgg ctgatgtgct gtgagcaaaa tatctccagt aataggtcat
    22381 cgattctctg tttctttgcg ttcgataaat tgaaaaaagt ttcttttggc atccacatct
    22441 ctcctttatc tctgattata cgtggatagc ccttcattgt catgaaggat tcattccttc
    22501 tttttctctt cttctgtcaa gtacccatct tccattcgat aaacgcgatc actccattga
    22561 atcatacgga catcatgagt aaccataata gtcgcttttt gtttctcatg agcttcttta
    22621 actagtaatt tgacgacttc atatgcatga tctgtatcca aactagctgt tggttcgtct
    22681 gctaagatca agcttggctc attatataaa gcgcgagcaa ttgccactct ctgtctttct
    22741 ccaccagaaa ggtctttagg ataactattt ttaagtgact ggatatctaa agattgcagc
    22801 aaggaatcga tttttgttga atcttctttt tttgaagtaa cttggtcaac tagttcaaat
    22861 tgttccttta ccgttaaaaa aggaatcaaa ttagagcttt ggagaataaa tcctatttct
    22921 ttaaagcgta aatctgctcg ttttttttct ggaagctcag ttagtgagtg accgttgatc
    22981 actatttctc cggaagtcgg tgtttgcaat cctcctgaaa tggtaaggaa agtactttta
    23041 cctgaaccag aaggaccaat gatgctgacg aattctcctt cctttacata aaaatcaatt
    23101 gctttcaaag ctgagacctt tgtatgacca gaaccaaatg ttttggtaac ttttttcatc
    23161 gttaatattt ttttcatttt tattaacctc ctattgctgt aattgggtct actttggtta
    23221 tcgtacgtat cgaaaacaat ccgcctagca ttgcgacaat gatcaaaatc ccactatata
    23281 gcaaccattg tgaccaaaaa acagcaaaag gcatcgcgct tggtaaaatc agactagtca
    23341 aataagcaaa caaaagagca agcagaacgc ctaccacccc aacaataaag gactgtgcta
    23401 ctaaagattt agcgatgaag aaatttttga tcccttgtgc tttcattact ccaaaaatag
    23461 cagtcttctg aagtgtcatc acgtacatga aaataccgac cacggcagca accactaaga
    23521 acaaaaagta tatcatcgca tttaaggtca tattttgggc agaatagcca ggaatacttt
    23581 caatgaatgt cccaatagtt aataactgta gacccttcga tgctttttct ttcgaaattt
    23641 tagctgaatc ctttattacg atcccattga tcggctgttc ttcttcagaa gcaaacggct
    23701 gttgtccata cttcaaagca gtccacgtat ctagggacgc atagatgacg ggagaaactg
    23761 tgtaataagt ttcaggaaag ataccgacaa tcgtcaaagg atcgtcgtaa ctgccaattt
    23821 tgatcgtatc tccaatttta tagcctgaat cagccagatt ttgagagatg atgatttcat
    23881 tctttgtttt aaattcatgc cctttcgtga gttttggtaa taaaaatgct tctttcgttg
    23941 tgccgaaaac tgtaacattt tctttgtttt ttcctttgat tgctccgctg tacaatccga
    24001 ttggtgcttt ctctcctcct tcaatgtatt tcagatctcc tcttgttaat tgtgaagctg
    24061 caaatacttg atttgcatcc tccgacaaaa caatctcttg tgcgtcccaa tcatcgattg
    24121 cttttttaaa ctcttctgaa agtccattcg ctagtcctga caacatgaat acgacatacg
    24181 caatcaagaa cataataccg acgatcaagc catatcgtaa tttagaatag cgaatttctt
    24241 tgatagctag aaacatttgt ttttcctccc tatgatagat tgatagtgac aaaatgtcac
    24301 ttgttgaata gtgtaactct aaagtgacaa tttgtcactt gtgttgtttt tttcttatca
    24361 ataaattgtc taagacatct ttcgataaaa ccttaacatg agtcataaaa aatttattta
    24421 agccttattt atgctttcca tgataaacta aagtgaccaa atcaatgagg tgggaaaatg
    24481 attaaactat tagtagtaga agacgataaa gtattatcca ataatataaa agaaatactc
    24541 gctgcaatcg gcgagataac acaagtttat gatggtagtg aaggactgtt cgagttggaa
    24601 agtggagttt atgatcttgc aatcttggat ttgatgatgc cggaaatgaa tggatatgaa
    24661 gtgttagcaa aaatacggaa agaaaaaata cagactccag tattgatttt gactgcaaaa
    24721 gatagtttgg aagataagat tgaaggattt gaacgaggtg cagatgatta tctaacaaaa
    24781 cctttctatc gagaagaact gatcatgcgc gtgaaagcat tattgaaacg ctcgctaggt
    24841 ctctttgatg aaaatgttct gaagtataag gatatcagtt gtgatttaaa aagcaatgaa
    24901 gtcttgtata aagaacaagt tttgcccata caaggaaaag agtttgaatt gctcgtttat
    24961 tttttacaaa ataagggaat cattttgaca aaagagcaga tatttgaccg gatctgggga
    25021 tttgattctg atacgacgat tacagttatt gaagtttata tgagcaactt acgaaaacat
    25081 ctgcgaagta ctggattgga taaagaaatc aaaaccttac gtaatgtcgg ttatatactg
    25141 caaagggtgt gatgcaatga atggagatat tacacgaaag caacgtagtc gcttttttgc
    25201 gacaaatgta gcagcatttg cttttatctt ttttcttttg ggattcatta ctttacagat
    25261 attaaaccaa tctgcataca gagaaacaga tctgtcatta gagaaaataa attttgattc
    25321 tcgaacagta caattagaga tacaaagata tcagcaaggc gatccctttt taaacccgac
    25381 tcaaacggaa caaaacagaa caaacatgga acagccatta atggaaccat caaatgaccg
    25441 ttttaacact caagtgatct tgtggtctaa aaatggtgag atattgaata aagcagctat
    25501 tggtgggcgt ttcaaccaaa tcaaagaatt aaaaatggat acgaaaaatc tgaataccat
    25561 ccaaaatatt gaattaaatg aagacagtgg ctcatctgcc ctctctttcc gttcgattac
    25621 gaaaaaagcg gagacgaatc aagatggcgt tgcctacatc caaattttag aaaatacaaa
    25681 tcaaatcaag aattcattag cgacttttcg cacgattatg attttgtgta tgattgtttt
    25741 ttggattctc tctattggta tcagttatta cttgtctagt ctaagcatgc gccctattat
    25801 tctttcttgg agaagacaaa aagaatttgt tgagaatgct tctcatgaat tgcgcacacc
    25861 gttaacgatt attcaaaata gtttggaaca tctttttacc aagcctaatc atactattat
    25921 tgaagaatcc gaaagcatcg ctcaagcatt gtcagagacg aggcgcctca ctggattgac
    25981 ttcggatttg ctgacaatcg ctcggaatga ttctgatcag cagctgcttt caaagcaaat
    26041 gatcaataca caagaatata tcgaacaact tgttaaacca tttcaagaga tggccatcat
    26101 agatggaaaa gagtttattc tccataacga tgcaacatta gaagtaccaa ttgatgaaaa
    26161 aaaaatccat caagttttgg ttattttgct ggataatgca ttaaaatata cgaatccaga
    26221 agataagatc attttggatt catctgttaa ccataaagaa tggctgattc acgtaaagaa
    26281 tactggccct tctatcagtg aagaagatca agcaaggatt tttgatcgat tttatcggga
    26341 agatcaatct cgggcgaaag agactggcgg atacgggctt ggcttggcaa ttgcaaaaca
    26401 ggtaatcgaa cagcatcatg gacagatttc tgtatccaat tatcttccac aaggagtcga
    26461 ttttaaagtc caattgccgt tgaagcctaa aaaataatgc ttatccctaa aaaagggctg
    26521 tgacaaaaat cttgtcacag cccttttttg ttgcgaataa acggtgttcc agaagtagct
    26581 tcttcggaaa taagccgaaa tttctttaag ctgaccactt ctgtcacaac ctctttagct
    26641 tacgcttacg attttttatt gcggtcttca atgtgtacat caccgcttgt gtcaatatcc
    26701 aattcttctc ggctaacatc ttctgtcact ttttcaacat cttcatgttc ttctttatga
    26761 atcccaactt cttcacgaat aactgtatgt ttgcttacat ctacttgctc ttctttgatt
    26821 gggatagtaa tcgtgtcatc ttccaatttt tcgtctgttc cctgtgaaga ttgatctgtt
    26881 actggtttgc gttcgatgac gatttcttca cgtttgaccg gtacttctac cgtttcagta
    26941 tcgttgacca catgtttgtg gatgtcgact tctccagttg tcacatcatg tgtatttaca
    27001 tctaattttt cttcttttag ttgaattgta tcatcatcta gatttggttt gcgatcgatg
    27061 tcttcagtct tgcttgtacc cattgtatca gtctcgccag gtacgtccat cggatcagtt
    27121 cctggtgtca tattcaatgg atccgttgta ttttgaaagc ctgtcgtgcc acctacgtcc
    27181 attggatctg ttccagtgcc agtttctaat ccagtcccca ttgtgcttgc tgggctaaca
    27241 gtatcttgtg tatccattgt atcttctgtt gtttctggct gacgatagcc ttttactgtg
    27301 atgacaaatt ttccatcaga aatatctcct ctatattgat ataaaggatc attttctggt
    27361 gtagccgtct cagaatcata ggtatcaaaa gaaaaagtat cttttatttt ttcccataaa
    27421 gaacggtctt cttcaccatc catccgattt tcatctgttt caacatctat accagacaat
    27481 ccgtcaaatc ctagtctaga tgctgcttca tcagtagtat aaataacgat atcatctcgt
    27541 tcataccctt cgttacgcaa ccgttcaatt actgataatg cttcttccct cgtactatag
    27601 cttccgatga ccgttaaatt tttctcttcc atgtaaattc ctcctagcaa atagatttat
    27661 gattggta
//
LOCUS       DARDHA010000019        27471 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000019 DARDHA010000000
VERSION     DARDHA010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.019"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(54..620)
                     /locus_tag="SDA10_000696"
     CDS             complement(54..620)
                     /locus_tag="SDA10_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG4239626.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(648..2150)
                     /gene="eat(A)"
                     /locus_tag="SDA10_000697"
     CDS             complement(648..2150)
                     /gene="eat(A)"
                     /locus_tag="SDA10_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEG4239627.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(2408..3529)
                     /locus_tag="SDA10_000698"
     CDS             complement(2408..3529)
                     /locus_tag="SDA10_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG4239628.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3655..4596
                     /locus_tag="SDA10_000699"
     CDS             3655..4596
                     /locus_tag="SDA10_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG4239629.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(4746..5642)
                     /locus_tag="SDA10_000700"
     CDS             complement(4746..5642)
                     /locus_tag="SDA10_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEG4239630.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            5911..7251
                     /locus_tag="SDA10_000701"
     CDS             5911..7251
                     /locus_tag="SDA10_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4239631.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            7469..7912
                     /locus_tag="SDA10_000702"
     CDS             7469..7912
                     /locus_tag="SDA10_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEG4239632.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(8416..9834)
                     /gene="nhaC"
                     /locus_tag="SDA10_000703"
     CDS             complement(8416..9834)
                     /gene="nhaC"
                     /locus_tag="SDA10_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEG4239633.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(10237..11460)
                     /locus_tag="SDA10_000704"
     CDS             complement(10237..11460)
                     /locus_tag="SDA10_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEG4239634.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(11491..12330)
                     /locus_tag="SDA10_000705"
     CDS             complement(11491..12330)
                     /locus_tag="SDA10_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEG4239635.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(12347..13027)
                     /locus_tag="SDA10_000706"
     CDS             complement(12347..13027)
                     /locus_tag="SDA10_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4239636.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(13029..14102)
                     /locus_tag="SDA10_000707"
     CDS             complement(13029..14102)
                     /locus_tag="SDA10_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4239637.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(14158..14396)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(14439..14672)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14749..15420)
                     /locus_tag="SDA10_000708"
     CDS             complement(14749..15420)
                     /locus_tag="SDA10_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG4239638.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(15507..18185)
                     /gene="mgtA"
                     /locus_tag="SDA10_000709"
     CDS             complement(15507..18185)
                     /gene="mgtA"
                     /locus_tag="SDA10_000709"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEG4239639.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            18844..19281
                     /gene="ndk"
                     /locus_tag="SDA10_000710"
     CDS             18844..19281
                     /gene="ndk"
                     /locus_tag="SDA10_000710"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEG4239640.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(19303..20169)
                     /locus_tag="SDA10_000711"
     CDS             complement(19303..20169)
                     /locus_tag="SDA10_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG4239641.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(20329..22422)
                     /locus_tag="SDA10_000712"
     CDS             complement(20329..22422)
                     /locus_tag="SDA10_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG4239642.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(23171..23794)
                     /locus_tag="SDA10_000713"
     CDS             complement(23171..23794)
                     /locus_tag="SDA10_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239643.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(23953..25350)
                     /locus_tag="SDA10_000714"
     CDS             complement(23953..25350)
                     /locus_tag="SDA10_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG4239644.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(25382..26353)
                     /locus_tag="SDA10_000715"
     CDS             complement(25382..26353)
                     /locus_tag="SDA10_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4239645.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            26727..27152
                     /locus_tag="SDA10_000716"
     CDS             26727..27152
                     /locus_tag="SDA10_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239646.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT         7883 a         5702 c         4789 g         9097 t
ORIGIN      
        1 ctaagcaaaa gcagtgcctt gaaatatttt atttaaataa cacaaaaaca taattactca
       61 taagaaacca attctgtatt atctaccatg aatgtaaaga acccctcatt aggcatcgga
      121 tgaaaataag cattgatcac tcttatcaag gcattatggc aaactaacag atagacattt
      181 tcttcgtctt cgatgcattc tttcaataaa ggaacgatcc gtgcatacac atctaagact
      241 gactccccat ttgggaatcg tacagcgaac gctaaacgtg ctttttgaaa gttctcatct
      301 ttgcttggca gtccatcata atccccaaaa tccatttcta ccaatcgttc atcaacggtc
      361 agcggcaagc tgagacgatc agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg
      421 gaatggatga ttttcgtgat tggaacaggc aaatctacta ccttttctgc tagtttctgt
      481 gcctgcatct gtcctttttc agtcagcggc acatctgccc gtccgcaaac taatccttgt
      541 gcgttccaag ttgtttctcc gtgtctcgtt acataaagca tgtgattctc tccttcgttg
      601 gcaaagttcc ccttaaccat accttgttgt tcttgagatt acaagggtta gattctctca
      661 agagaaataa tctcagtaga tactttatcc aggaaggttt gatcatgttc cactagtaac
      721 atggcaggtt tcacgttcaa gatcagttgt tctaattgtt cttgattgaa gacatccaaa
      781 taattcaatg gttcatccca tgtatatagt tcagctggct gtgataaaga tttagccaat
      841 tccacttttt tccgttggcc catactcatc tgctcgatct tgttatgaaa aacatctctt
      901 tccatcccaa gctttcggag gttgttcaaa aatgcttggt agtctacttg gtttttctct
      961 gcaaattccg ccaacgttcc tcgattgtct tcataatttt ggcttgcata actaatgctc
     1021 agatgttttg gctggtattt ttctcctata accttgccgt tgaatgcccc cagaagataa
     1081 tggataatgg atgactttcc tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc
     1141 tcgattgtaa aatgaattgg ctcgaataac agattttcat accctaattg aagatcttct
     1201 acgcttaaaa gtcgcttatg gtgagacgct tggctattca tcgtcaaaga atcgatatat
     1261 tcgatatctt ttaatagttt ttctttctcg ctgatctggg tctccatccg attcacgatt
     1321 gctttggatc gtttcatcgt ccgtgcagca tcagcaccca ctgctccttt attcactcgt
     1381 ctagattcag tatcgatgaa tccgacttgt ttctttgttt tatctccttc tcgggaacga
     1441 gaccattcgg ctttttcagc tgctgttttc tttagcctac tgacttcttt tttcaatttt
     1501 tcattttgag ccatttcaaa ttcatcacga agttttttct gttcttcata gatagagaaa
     1561 ttcccttgat aaagttccag ttgacttttt tcgattgcta aaacatggtc cactacttca
     1621 tcgataaatc cccggtcatg gctgaccacg atgaagcctt gttttttctt tttcaaataa
     1681 gccgctactt gttttctacc agagatatcc aaatgattcg ttggttcatc gattaacggg
     1741 aaatgagtgt catccacaaa taaaagtgcc agtaggactt ttgtcttctc tccccccgat
     1801 agtgtgctga attctctcca taagatttca ggatctgttt gcatcaattg gagctctctt
     1861 tcgatttccc atatctcaaa atccgtaata tcatttaaca cgtaataggt taaacgttct
     1921 ttatcttttg tctgttgcgg gaaataggca aattcttgct gatggattac ttgcccttga
     1981 taaggtagtt tgttttgtag aatattcagt aaagttgtct ttcctcgacc gtttcgtccg
     2041 ataagtccta gtttccattg tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg
     2101 ccttggctgt cgtagccgaa tgtcagattt tttatttcga ttttagacat tttccatcac
     2161 ctcgtaaaaa aagaccagtc ctattttcag aactggctaa agaggatgca agggttggat
     2221 acacacaaaa agacccatac atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc
     2281 ttccttgtct ccacaaggta cagtctgaat gcagctttca tcaatcgact taatttttca
     2341 atgcacggat ccctcacttt gtcctatatt tgaaattacg atagcataaa gaaaatgatt
     2401 ttgtcaacta cttcgggagg atggtgaaaa atctgctaag tgtctgtcct aagatttttg
     2461 ttttttctaa tgtttgcctg tgttccattg cttcttctaa acctattgga cccaaatgaa
     2521 tacttaagac aattgggaga aaatcagaaa gtccttctag ctcttggtct ctacttccgc
     2581 aaatagcgat tattggtttt cgatattttt ttgcaagcac tgctacaccg tatggaactt
     2641 ttccgtggat cgtttgctga tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt
     2701 cgatcttttt ttccaatcca gtgatttggc tgactaaatc gaaaccagaa accatcttcg
     2761 ctcccaacaa aaaaagggct ccaccaatac cgcctgctgc tccacttcct ggaatatcat
     2821 ctaaatccac ctcccagaaa tcttttattt gctgagcaat ctttcctgct tgtttatcta
     2881 agaaagctag cgtcgtatct attcctcctt tttgtcttcc aaattgccaa gcagctcctt
     2941 gttgacctgt atatggattg gtaacatctg cagcgataat caattttact ttagaaacaa
     3001 gaacgtttct tcgatcaaaa tgaagttttt tagtagataa taaaggattt ccttctgtgt
     3061 acccttctaa agaagcaccc aaggattgga gtaatcctag accaccatcg ctacacccgc
     3121 ttcctcctaa agtgacaata atttgttcta cttcttgaag cagtgcatct ttaataagtt
     3181 ctcctaaggc ataactggaa gcaagtcgga tagtttgatc cgttggttta tcaagtagat
     3241 ctaaccccaa tgcttgtgca gattcgatga ctgcggtgtt ttttccttgc caagttgtca
     3301 ataaatagct gatttttcgc tgacggaata agagatccat cccatcaatg gtacgccatg
     3361 ttcccctgag cgcgtaatag attgcttctg cagtattttc tccgccatct gcaatcggca
     3421 cattgatttt tcttccttta cgatcttccc agccatttag aactgcctga tttagttcaa
     3481 atgaagaagc acttccttta aatgaatcaa ttgccgccaa aatatccatc tgtatccctc
     3541 actttcttct taagtaagac actttttttc atttttttca aacaaaacaa tgctattttc
     3601 ttccattttt ctatatacta aaagggaact cattgaataa aggagagaaa aaacatgaaa
     3661 atcgcaatag ctggggccgg agcaatgggc agccgaatcg gattgatgct acatcaaagc
     3721 ggaaatgagg tcctcttgat cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat
     3781 gggttgattg ctgactttaa cggtaaagaa gtggtcgcaa aactgccaat ctattcacca
     3841 gaagaaatca tcgaaagcaa cgaacacgtg gacttgatcg tagcattaac aaaagccaat
     3901 caattagatg atatgttttg ttcgatccaa tccattatta ctgacaatac ttatgttttg
     3961 tgtttactaa atggattagg tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat
     4021 atcttatttg ggatcacgat gtggacagct ggtctagctg gtcccggaaa agtcacattg
     4081 ttaggtgatg gagaaatcga actagagaac ttggaaccag aaggcgaagc atttacaaaa
     4141 aaagttgtag aagtctttca agaagcaaat ttaaatccga tctatagcca taacgttcgt
     4201 tattccattt ggcgaaaagc ttgtgtaaat ggaacattga atggtttatg cacgattttg
     4261 gattgtaata ttgctgaact aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc
     4321 gtatctgaat ttgcttctat cgcagcaaaa gaaggaatca tactagatca agaagaagtg
     4381 taccaacata ttgcttctac ttatgatcct gacaacatcg ggttgcatta cccatctatg
     4441 taccaagacc tgatcaaaaa ccatcgtttg actgaaatcg actacatcaa tggtgccatt
     4501 tggcgaaaag gacaaaaata tgatattgct acaccatatt gtgccttttt gacccaatta
     4561 gtccatgcaa aagaaggaat ccttggcgca gaataaaagg ctctctgatg agaatcagct
     4621 gaatcagcag ctggttctct ttttttaagc ccaataaaag atcaaaaaga aaacacaact
     4681 ctcatgatgc cataagaatt gtgcttgttt gtttcagtac ataatctctt caaaatgtag
     4741 gttttttatt tgttttcaac agaggagagg tcatattcgt attgctcata aatagtggaa
     4801 tcgttagtga taaatcgttt tgcccaattt tctgcatcat aaaattccaa tagctctgat
     4861 aggattggct taaatggtgt atgcagaaat tccggtaatt catctaaatc acgttctcga
     4921 agcaaaaaaa tattttcagg acgataaaaa tgataatgag caatcgcttg attatccgtc
     4981 actagtttct tttgaaaata catggattca aatgtccgca aagtcactcc ttgctgaccc
     5041 tcccgcaaca gttccagaat cccgcgcgat tcccctgttt ttttcaggta ctcttcataa
     5101 gtaagatatc caggttgttc attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca
     5161 tctcttttta taaattcctg cttatagctt tctgctcgtt cttttctccc attattcgtt
     5221 gcaccaaaaa atagatccgt ctttggttcg ctagaagaaa aattcatatg tttcgaataa
     5281 aatgaactat tatgtttcag cccaagttct tttgcttcca aaaaatcaaa atgataaatc
     5341 tcatcgattg ccggttcatc ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag
     5401 tgattccaaa aaaataacac aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc
     5461 tccatatcaa atccataatc taaaaaaacg atcagctgat aatttgccaa ctgttgtcta
     5521 gctgatttaa tgaattccga ctgtagtaaa tcttttccgg accaaacatc taaagagcgg
     5581 atggaaggca aaaaataaag tgatttattt tttgcaacta gcagtacgtc atttttgttc
     5641 atcttttccg cctcactttc tctgtcactt tttcctatga tagcctgttt aaatagtaaa
     5701 aagccagtaa aacgtttcct atatttgtat gaaaatttaa catttgtatg aaaatatgat
     5761 gaaataaatc attcttctat taaaattcgc ctaaaagttt tctgaatagt tgtaattgtc
     5821 tgaaaatttg ttatcattag tcattgttaa atattgctat ttattcttaa tataaatttt
     5881 gattgtgaat ttaaaagaaa gaggtcattt atggaagata aaaaattgaa aaaacaactt
     5941 agctccagac atatcaccat gttagcttta ggtggagcaa tcggtgcagg tctatttaaa
     6001 ggaagtggcg aagcgattgg gatcgccggc ccttctgtat tgattgcttt tttgattggt
     6061 gggctgattt tatatatcgt gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac
     6121 acgcatcacg gattatctgg tttgatacgc ccttatctag gtgctcattc tgctgatttt
     6181 actgattggg tctactggtc tatgtggatt atcaatatca tcgctgaagc tgttgcagct
     6241 gcttcctttt tgcagttgtg gttcccgcac attccagcct gggtatttgt ttttatgttg
     6301 gctgttttaa ctacgatcat caatctttat tcggtacggt tattcgcaga aacagaatat
     6361 tggctggctt ttgctaaaat cagtgtcatt atcttactga tcatttttgc tacttatctt
     6421 gtcggtcagc aaatgttggg cacaggcgtt ttccctaccc ttcagcaatt gactgaccat
     6481 ggtgggttta ctcctcatgg tatgaaggga atcatcagtt ctttgcttgt agttatctat
     6541 tcttacggcg gttctgaact gattgctatc acggtaagcg aagcagatga tcctaaacgt
     6601 gcgattccta aagcaatcaa aggtgtcatg ggaagaatca tttcctttta catcattcct
     6661 ttgtttttgc ttttgattct ttttccttgg aatactttag caggcacgaa tgtcagtcct
     6721 tttgtgatgg tatttgaaaa aatgaatatc ccatttgcag cggatatcgt caattttgtg
     6781 attgtccttg ctttgttctc atctatcaat tcgggtgtgt atgcatcttc aagaattttg
     6841 ttcttccgtt tgaaggatcg aaaaggttcc tctagaaaac tagctgtttt aaacaagcat
     6901 caagtaccgc aacgtgctgt tttattttgt accagtgttc tttatcttgg tgtagctctt
     6961 tcttattttg tcggtgacaa attatttggc tatctggcag gttccctttc gtatacagta
     7021 ttattgatct ggatcattat cagtgcggca tcctttgtac tggcactaag aaaaggaaca
     7081 ttatgggata aaggaatcag cttttttgca ttagctgtct taggattgat tttccttggt
     7141 atcctgttca cgaactccat tggggtaact gtattgactg gcctgcttta cctgttcatt
     7201 tatttcagtt atcaaaaaaa gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag
     7261 aaaattttat tttatttatg tggaatagat ggagctattg aagaaaggag ttgtgacaaa
     7321 agtcttgttg caactccttt ttccgaataa acagtctcat gacctcttgg taaacggtgt
     7381 tcaaaaaccc tctgtgcaaa ataattgatt gttctccatg ctcatgacat gatagaagaa
     7441 aggaatgaaa aaaaggagtt tgttttaaat gaaaaagtat tggaagtttt tgttgatgat
     7501 cggtgtttca acggtagtta tgtttggtat gatgtacttg aatgtctatg cgctcgatca
     7561 tatcttcttc agtcgtacac gagtattcat ggcgttgatg atgggtgcag tcatggcagt
     7621 catcatgctg ttgttcatgt ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt
     7681 agccgctagt cttcttgttt ttgccggttc cttgtttatg gtacgaagcc aaactactgt
     7741 caacgacaca gcttggatga aggccatgat tcctcatcat tctatcgcta tcctggcaag
     7801 taaacgagct gatttaaaag atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc
     7861 acaagaagaa gagatcgaca aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc
     7921 cgtccaaata aaaaaagagg atgttccaga agttatcagc ttcaagaaat aagaagaagc
     7981 tacttctgga acgccgtgaa taaggggttg tgaaaaagct gccctgcatc aagtaataag
     8041 aacagccaaa caaaaatagc ttctcctatt tttcgtttgg ttaggcttat taccgcagat
     8101 gcaagctttt gaacaccgtt tattaagagg ttgtaaaatc agcgttctga cctgagtatt
     8161 agagagacaa atggaaaaat agttcctcat attttgccat tttttgagct aataccgagg
     8221 gtcagctgat tgaacaccgt ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat
     8281 taagaaaaat tttgtgaaat ggctttccac atttttgtat aaatttggct taataccgag
     8341 aaactggctt ttgaataccg tttattcgta aaagggattg tgacaaaagt cttgtcacaa
     8401 tcccttttta tgtttttatt gttctaattg cgctgtcttg cttactgctt cttgcggttt
     8461 cacaaatttt tttgagattc cccaacctgt gaaggcactg accatcgaaa acacaggaga
     8521 aagcaaactg aaaaatacga atggcaagta agtgagtgta ggaataccaa atgtacttgc
     8581 tacgaaactt ccggcaattc cccaaggcac taagtagttg attactgttc caccatcttc
     8641 tagcgttcta cctaatacag ttggatcaaa tcctgctttt tcataagttt ctttaaatgc
     8701 attaccaggc agaataacag ataggaattg ttcaccaata aaaatattta ctccgatact
     8761 tgtcatcaat gttgtaaaaa tcaaatttgg tgtgttcgtc atctttcttg atactcgttc
     8821 catcacggtt gagatcaagc caaattcgat caacaatcca cctaatgaca aagttagaat
     8881 aatcaatgac acagtaggca tcatactttc gattcctccg cggctaagca gtgaatcgat
     8941 ttgttggttt ccagtatttg aaacaaaccc tgattcgatg accccagcca acttcgttaa
     9001 ggaagtttgt gggttttgaa taaaaatcat tcctactgct acagcgatat tcaacaaaat
     9061 cgttaaaatt gccggcacct ttttccatgc acacagtaac attaaaaata aaggaatcag
     9121 tgaccagatt gaaatagtaa agtttgcttc tagaacatca gtcacttcac gaatagatga
     9181 aagacttgcg gctttacttg tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa
     9241 tgccgggaca gttgaccaca tcatatgctt gatgtgtttg aacaaatcgg catcaacaat
     9301 agctgctgct agatttgttg attcggataa tggagacatc ttatctccaa aaacggcacc
     9361 tgaaacaata gctccaacaa ccagcgctgg atttaagcct agtgttgtcc caataccgaa
     9421 aaatgcaatc ccaatcgttg acataacagt aaaggcactc cctacagcgc tacctacgat
     9481 tgcacacaca acaaatacag aagggacaaa ccactggaca ctgatcaatt tgaacccaat
     9541 gaccattaga gttggaatga tccctgcttg tatccataca gcaatcaatg aaccgactaa
     9601 aataaaaata aagattggga tgattcctgg tttgatgcca ttgatgattc cttcgtgcag
     9661 ccaatctgtc gggtatcttt ttatcactgc atacaacatg accattccga tcgctgctaa
     9721 gatcgtgata ttaggtgaga cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat
     9781 aaccattaat acaagtgttg cttctaaaaa acttggtgtt cctttttttc ccatgataat
     9841 ctctcctttt ttagttgttc ttatgttcta aaaaagttaa gatatcggat tactccgccg
     9901 caattcatcc gcgattcctc ctttataaaa ataaaaagac ctgttttaaa ctaaggacga
     9961 atgactccgc ggtaccacct taattaaaaa cagatctgtt tttcactttc aaacttttat
    10021 tcaaatgtat ggactttcca tgtaattcgt caattaggct tgatcggttc acagcgacca
    10081 ccgactttct gaacaaaaca cctaaccgct actgcgtgaa aagttgactt gtacgaagtt
    10141 taccttccat aagatcattt gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat
    10201 tatttttata actttcacac acaagtttat tttttactag aaagtagcgc cctcttgttt
    10261 ttttcttgct actatttctt gatgttcttg cttgattctt tctaatagct ttggctcttt
    10321 caacagatca agagctgtat tggcgagtag ttctgctgca atcgcaatag aagcaaggcc
    10381 tttgtcactt ttagcagcag ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga
    10441 gattgctacc gtaggctgga ttgttggtat gacttggctg acatttccta catcagaaga
    10501 accgagactt gttctttgtt ccgtttctat ttcttcatct ggaacacctg cttcttttgc
    10561 atgctggaag aataagtcat caaaagaagg agtaacgatg acatcatcaa ctgcattttg
    10621 gaacaatcca aactcaaaag tcgtgcccgt acttatcgca gcacctcgta cgatattttc
    10681 cactttttca tatactttat ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc
    10741 agcagcgtaa tctggaatga cgttggcagc tactcctccg tctgtaatga tcccatggat
    10801 attgacatct tttggcaatt gcaagcgtag agcgttgatt ccattaaaca cttggatcaa
    10861 tgcttccaaa gcatttactc ctttttcagg tgctgctgct gcatgggaag cgacaccgta
    10921 aaatttgatg tcgactggat cattcgctaa agattctgtc gttttcccat aacgaaaagc
    10981 tggatggacg cataacgcta catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc
    11041 tttagctgat ccgttctcgc ccccttcttc ccctggggtg ccatatacac ggatttctcc
    11101 accagtttca tcaatgaatt gcttcactat gctggcggct aatacggagt aagtaccaaa
    11161 caaattatgc ccgcatgcat gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc
    11221 aattgtggga ccgggtttat tggaaatata acgtgcatca aaggctgttc ggtgtccagc
    11281 tacttctttt tctacccaaa acctttcttt ttctaattgt ttaatcaacg ctgctgagga
    11341 ataaaactca tagttactta cttcaggatg ttcgtggata tctagagcaa gtgcttgata
    11401 ttctggtaaa cgatattgga ttagatcctt caattggttt gttgagattg ttgttgtcat
    11461 aaaaaattcc tcctatgcta gtcatttttc ttattttctc ccgaatgttt cccatgcagg
    11521 cacgcttgat ccttttgatg ttttctcgat tactttgact gtgtcttctg tttgataagc
    11581 gtctactatt ttttgatacg ttttattgtt ttcatcttct tttcttgcaa cgatgatatt
    11641 cacataagga cgtgcatggt catccactgg ttctaagaag atggcatcag aagcaggatt
    11701 aaatccagca tctacagcta caccgctatt gatcactgaa atgtctacat ctgacaaagc
    11761 acgtgctgtt tgggaagcat ctaattcaga aatgtctaga ttttttctat tctcggtaat
    11821 atcagaaaca gtgggcgctt gtccttttgc cttatccact ttgatcaatc cagcagtttg
    11881 caataacaga agagctctgc cgccatttgt aacatcatta gggattgcta cttttgctcc
    11941 gtctttgata tcagctacat ccttcaaagt gttagaataa attcccagcg gtgcattgac
    12001 agtatttccg atcgatacta aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa
    12061 ttgatgttgg aaagaattca aatcaatcga tccatctgct aaagccacat ttggttgatt
    12121 gtaatctgta aatttcacgt attccaaatt aatgttctcc tttttcaaac ggtctttcac
    12181 gttgtcccat acatctgtat cttctccaat aatccctagt tttacgatag tttctttttt
    12241 gtcagttgta tctgcttttc cttggctaca ccctgccaac caaataattg ctacaaatgt
    12301 aatgactaca aaacgaataa tttttttcat tttttctcct ccactttcaa tgtgtaattt
    12361 ttttaatcag ttgattactg ataaattgac ttaaaaaaac taagattaga atcaataatg
    12421 tagccacaat cgttacatct gtttggaatc gattataccc tctcgatata gcaagattcc
    12481 ctaatccgcc tgcacctact gctccagcca tggtagtcag tccgatcacg ttgatgatag
    12541 tcaacgccga gacccgaatg ataccaggca aaccttctaa caaatacaca cgtaaaatga
    12601 taccgattgg actggttccc atcgcttctg ccgcttcgat caccccagga tcaacttcta
    12661 gcagtgcgtt ctcgatttga cgagcaaaaa acggaacaac tccaaaaatc aatggcacta
    12721 aagctgcttt cgcaccaatc gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag
    12781 aaagcaagat aatgaaaggg atggaacgaa tgatattgat cagcttatca agtatttcaa
    12841 atagcagacg gttagctagt aatccattag ggcgtgtcac tactaacgtc acacctaaga
    12901 tgattccaat catcccgcca atgaatgctg tccaaaaaga catatatagt gtttgcttcg
    12961 tcgcttcgac aaattcatcc ggaatcaaat aaacattggg caaatatgtt ttaaagaaat
    13021 cagtcatttt atcttcctcc ttcgatcact ttgatacgac tatctaaatc gtttttttgt
    13081 aaaatattta tttttacacc agatttgatc aaataatcca atgctttttt ccgctgtttg
    13141 gtttctccag ctaaagtaac gatcaaactc cctaacggcg tttcttgtaa taactctaca
    13201 ttgccatata aaatattcgc tgttacttga tatttgctgt acagatgagc gattagaggt
    13261 tcgttcgtct gatttccaat ataacttaat tccaccagcc atttattggc gatctgatcg
    13321 gcaaaagcag aatgggcaat gatcgtttct aatgcttggt caagatgagt agctgttcga
    13381 ataaaatctt tggtcaattc ttcttcagga ttgctgaaga tctggatgct gctacctttt
    13441 tcaacgattc ttccatcttc cataaccgct accttgttgc agatttcttt gacaacttgc
    13501 atctcatgag tgatcaggac gattgttaaa cctaactggc gattcaattt ttttaacaat
    13561 gcaaggatct gaagggtcgt tttcggatca agtgcactgg ttgcttcatc acataataat
    13621 accttaggtt cattcgccaa agctcgtgcg attgctactc gttgtttttg tccccccgac
    13681 agttgtttag gataagcaga tgctttttct tccaaaccga caagttccag tagttcattc
    13741 actttttgac gacgttcctg tttcgatttt ccagaatact ttaaagaaaa atcaacgttg
    13801 tcaaaaatag ttcggctttc cattagattg aaatgctgaa agatcatacc gatggccttt
    13861 ctttcttttc gcaattcttt gggagagagc tgactcagat tcgtctgatt gacaatgact
    13921 tctcctgctg ttggtttctg cagcaaattc atcacacgaa ctaacgtact tttccctgca
    13981 cctgagtaac cgacgattcc ataaacatcg cctttttcaa tcgttaaatc tacagaatct
    14041 actgcctgta tctctttttc tttttgtttg aaacatacag aaacatctcg caattcaatc
    14101 attaaaattc ctccttatct aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt
    14161 gaggacgctt ttttgcgtgt taccacctca tgttcaagct gtgttcacac acaactcctc
    14221 ttcaagtacg atcatactct agcaagatat cggtcgcagc cgtcatcgcc ttacatctat
    14281 gctcgggatg aattgctcga tggccatttt ccaatagtcg ttccttgctt cttctcagct
    14341 accgaagctc tctgtcaaag gttcttctat cgtacttacc atttcaatgc aaataatatt
    14401 caaaaaaaaa gtccttttac taacataaac tatgtcagta aaaggacgac gtatcgcggt
    14461 accacctttt ttcggaagtt tccttccctc aagcccaaca tctcaaaaag agagattggc
    14521 gttcgtttta acggtcgaac agttccgaat ttgcctactt atcgacaaat tattctccaa
    14581 ggccattttc agcttttctt acttgactct tctcaccagc cagagcctct ctgcaaagtt
    14641 tccaaactta ctttcctttt caacgaaatc gatattttgt tgttgggatt aatctacatg
    14701 aggaaaactg aaaagtcaag tacgaaattc aaattatttt ttctatattt atttttctgt
    14761 ttttttattt aacatttcaa taacagttcg aagatctttt ccttccgcaa gaagtttcaa
    14821 tttttccttt tcttcgacgg tataatttcg gacaaaactg gaatcaggta tcaaaacaac
    14881 gcctaatcct gctcgtgaag cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc
    14941 tgaactagct tctaatcctg ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt
    15001 gttctccgac acttcgtctc cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg
    15061 atccaagata tctgttgctc ttttcgtaac agtggtcgta gcaatgccta gtttcatatg
    15121 acgttctttt aagtctgcca atatttcttt tgcatatggt tttaagtgga tggctccgct
    15181 tgtcagctgt tgataaaaat attcttcacg cttcgctcgg acttcctgta ctttttcagg
    15241 gctacctgct gctttcacta aaatatcaaa actttgtttc cagttttttc cgccccaagc
    15301 gtcaacgatt tcttttggga tatggatatc aaattgctga aatccccaaa gccagccatc
    15361 acgatacgtt ttttctgtgt caactaacaa accatctaaa tcaaatattg cacctttcat
    15421 ctatacatta atcctttcca aaacaataca catagtttag cacgactggt ccaaaaagaa
    15481 cagaagcaaa gaaccagcct tctcgtttat aagagttctt ggaatcgacg gatatagatt
    15541 ttcttcacag ctgtcacaag cgccagataa gcgatgactg tagcaaataa ccataaccag
    15601 taattgccag gaagtggcat caagcctaat tcagcaccaa aacttgtaaa aggaaggaca
    15661 gaaccgacaa taattcctaa tgtagtgata acaaacatgg caaatgttgc gttgctttgg
    15721 ataaatggta ctttcggtgt acgcagtgca tggagcacta aggtttgtgt ccatagtgat
    15781 tcgacaaacc atcctgcatg gaataatgcg atgaaaatca ctcgctgtga actatccaat
    15841 gtatggaaac ttccgcccac tacagcagga caaataacaa aatacatcaa caagtaagta
    15901 gtaatatcaa atatcgaact tgttggacca aagtaaacca tgaattcccc aatactagat
    15961 gcattccatt tttttggctc gtccaaatat tctttatcca tccgatccca tggcaaggaa
    16021 atgcaggaaa catcatagat cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa
    16081 aatggcagga acgtacttgc aatcagcacg gaaaacatat tgccaaaatt agaacttgct
    16141 gtagccttga tatatttcat gatattccca aaagtttctc gtccagaaag aaggcctctt
    16201 tccaaaatcg tcaaatcttt ttctaaaagg atcacgtctg cagattcttt cgcaatatcc
    16261 acagctgtat ctacagaaat accgatatca gccactttca ttgcaggagc gtcattgata
    16321 ccatctccta aaaatccgac agtatgacca tttttacgta aagctgtcgt caaacgtgct
    16381 ttttgctgag gattcaattt tacgaatact tcatggtctt cagctacttg tgctaattca
    16441 tgatcactca tttccgagat tttctcaccg gtaaccaatt cattgatctc tagacctact
    16501 tgacggcaca ctgaacgtgt aactaattcg ttgtccccag tcagaacttt tacagctaca
    16561 ccatgttctt tcagagcttt caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa
    16621 gcaagataac caatcaaaac catgtctgac tcatctttga ctgaaaattc tccaacgacg
    16681 cttggattgg tcttttgtgc gacaccaatc actcgtaagc catcttcatt aagtgactgt
    16741 acttttgcaa gtacggcttc ttttcgttct tttgttaatg gtgtaacagt accgccaaga
    16801 tcaataaatt tagaaatgtt cagcatttct tcaatcgcgc cttttgtgat catttgtgtt
    16861 ttaccagctg gatcagcaac cacaacactc atacgacgac gttcaaaatc aaaaggaatt
    16921 tcatccactt tttgataatt gatcttatct gtatctaaag tttctgttgc ttcatcaatg
    16981 atagccacat ctaacaaatt tttcagccct gtttgatagt agctatttag atacgcatgg
    17041 cgcaaaacac gatcatcttc atttccttca caatccatat gatattcaag aatgatttta
    17101 tcttgtgtca atgtccccgt tttatccgta cataacacat cgattgcacc aaagttctgg
    17161 atggcattca aatttttgat cactgttcct tttttggcca tcacacttgc tcctttgacc
    17221 aaattcgtcg taacgatcat tggaagcatt tcaggtgtta gaccaactgc tacagatagt
    17281 ccaaaaagaa aagcttctag ccaatcccct tttgtaagcc cattgatgac gataactgct
    17341 ggagccatca atgacataaa tttgatcagt agcatggatg ttttatggat gcctaattca
    17401 aagcttgatt gaattggttt ttcagatagc gtttctgcta catgaccaaa tagtgtttca
    17461 tttccaacag aaacaatgat tccttccgca cttccactga tgacattact tcccataaag
    17521 acaagatttt cgtaacttgt ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt
    17581 tctactggat agctttcccc tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac
    17641 aataaacgca tatccgctgg tatcatatcc cctgctgaaa gccgaacaag atctccacag
    17701 acgatttctt ccataggaat ctctgtgtac tttccattac gttttacagc tgccgttact
    17761 ttcacaagtg atttcagttt ttcagccgct tgattggaac gtacggactg aatcaacgtc
    17821 attgtcccgc tgacgataac catagtgaag ataatggcta caccgaaaag atctttatct
    17881 ccaggagctg ctaatacata ttccgtgatg aatgaaataa ttcctaatgc gatcaacact
    17941 aatgtaaatg gcgtgatgta tgctttcaat atttctacaa tgaatggtgt ttttttgcca
    18001 tatgaaatcg cattttcacc gtaattttct cttgaacgtt cgatttgttc ttctgtcaat
    18061 ccatttgctg atgcatggaa tagttcaaat aattcttgtt tcgtttttcc agcaaaagct
    18121 gcatagattt tttgtgtatc tttctttttt tccatctcaa tctctccttt gtgtccaaaa
    18181 gacagctgtt ctcgctgctt ttggtcttta gctattcatc ggatcgatag agaaaaaact
    18241 gaaaggggct gagaacagat agataatagc cctggccttt cctctagatc cttgtttttc
    18301 gaatgactgt catccatttg ttctcatctc ctttactaag taattttaat tcatgacttc
    18361 caatcatttt tcgaatagtt atttttccaa ttagaccacc tcctttttat ttttcaccga
    18421 tggggatttg cggcaacaaa aaaacctctc gccaaatact cagcgaaagg tttgaaaaag
    18481 catattaaaa aaagcttctc agatctcatc gttgagtttt gacactgtac aacgtaaaaa
    18541 gcagatacct gcttttagct accttatgaa aagccttatc tcgacaattc ctgttctacc
    18601 cattggcgtc tctcgacgtt tttgggcagt agcctatgtt tgtataggag cctcacctaa
    18661 caaatcttat tcggttttcc ttcgttaata taacaactaa aaaagaggat gtcaaatcaa
    18721 ttttccatgt ttttcatact ttttttaaaa tacacacgtt atattcctgt ctcacgcggt
    18781 tttatttgtc tcattttcca cctcccgcta taatagaaat atagtaggaa acgaggtgct
    18841 gttatggaaa gaacacttgt tattatcaag ccagacggcg tgagacgcca tttagtcgga
    18901 agcatcatcc aacgttttga agcaaaggga ctggccattg ctgaaatgaa gtttgaaact
    18961 atgactcctg aattagcaaa agaacattat cagcacttga ctgagcgcag tttttttgat
    19021 gaactgatcg attacatgac ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa
    19081 gtgatcgata tcgtccgtaa gatggttgga gcaacaaaag ctgccgatgc tgttccaggt
    19141 acgatccgag gagactacgc gctgccgggg actgaaaaca tcattcacgc ttctgattcc
    19201 cgtgatgctg cggtaaaaga gatcgcccgc tttttcccgc cattcgatat aaacaaaccg
    19261 tctgaaaaag cggcgaagtg atacaaattc taaactgctg gtctagttgc tgggttcagc
    19321 aatggccagc agttttttct ctaaggacct ccgttgattc tttcctaaca tatattcgag
    19381 cactgtgaag aattcttggt agtctttcgt cttttcttcc cctaaagcag cagaaatcaa
    19441 tctttgataa taatctgcca tcaatgtttc atagatcact gtacttcttt ctttatgtgc
    19501 taagctccgt ttcatatctt cattcacact acgtgccttt ttcaaccgac cattttctat
    19561 ataaaacgaa gtcaggttca ttaaaaaagt aaagagatac tgctcatgat tgttcaactg
    19621 tgctaatcgt cgcattcttt ttttgatttt caaataattg ttctcaatga accgatcact
    19681 gaatatccaa ggaaaatcag ctagaataga tagttcaaat tgaccatagt ctggtatctg
    19741 catcaagtat tcttcaatga ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga
    19801 tacttctgtt ttttcgacca tgtgaagatt caaaaccaaa taatacagat cttctgtttt
    19861 cttaaaatgc ctacggttat tgctgatctc tttataagga tcaaagctgt cttcttgtac
    19921 ccgatctagc attcgtcttt ttttctttaa ctttattggt tcatccagct ggtttcgcat
    19981 ccattcatat tctgcaagtg tcatgttcat tcgctctaaa aatgatctca atgttacaaa
    20041 tgacattttt ttcactgaat ttgtctcaat cccgactaga gaagtcttaa aacagattcc
    20101 ttccgctaat tgggactgtg tcaacttctt gttgactcga agtttcttga tcaattcacc
    20161 atcatacata atagttctcc tttttaattt aaatttttcc acattcaata aatagatacg
    20221 ttaaaaagga aaaatacacg aaaaaaataa aaaaatgttt ttttcataaa aaataccaac
    20281 cttcaagcat tagaatgttg tctaactact gaaggttggt ttgattgttt attgattatt
    20341 tttttgctgt tcaacgtctg ttcttgttac tggatataag ttggttgttg ttttattacc
    20401 attttttgaa aagacacttg tcgaatgttc gcctttttgc ttttcgattg cttcattacg
    20461 ttccgcttca tttttatagt catatttttt cggatcaatc gtctcaaatc cgtttggcac
    20521 ataaaaccgc aagagatttt cctgattcag cttatctgaa agacttaacg cagtcttgac
    20581 tttttcctga tctttggaga tttcctcatc gattcctgct gctttcgtat cgatgatttc
    20641 tcctgtctga ttattgtaag cttttcctcc taggacggtg tatttgggtg tcacaaagtt
    20701 cccatttcga aaagcaacta cttgatcatg ctgagaagat aataagtctg taccaaaatg
    20761 aagataatct ttatcatcga ttcccaatag atgtagtaat gtcggcaaaa catcgatttc
    20821 tccaccgtat tcatgattga ctgtcccttt agtataacca ggcatatgga tcatcaatgg
    20881 cactcgttgc atctgtgcgt tgtcaaattc atcccatgta tctggatctt taccaagagc
    20941 gcttgctaaa tctttattat ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa
    21001 gattgaattt tggtaaatac cagatttttt caaatacgta aaaaattgtt ccaatgattg
    21061 atccagataa tgtgccgttc gaacataatt atctactgtg ctattgccag tattcaaaga
    21121 tggaaagtcg aaattcttgt catccgtata ataaggcgtg tggttggtta cggaaagaaa
    21181 tttcgtgtaa aacggctgtt gtagatgctc aagatattgg gcagattctc tgaataaatc
    21241 tttatctaat atcccataac ctaacgtttc gtcggattca tcaaaataag atcgatcaaa
    21301 gaaattgtca taacctaagt ttttatatac atgatctcta ttccaaaaac tagctacatt
    21361 cccatggaaa acagcgcttg tatagccttg cttttgtcca aggatcgctg gtgcggcttg
    21421 aaagacattg tctgacccaa gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc
    21481 agtttccaac atattttcag catcacttgt ctttccctgt cctacttcat gaaagaaatt
    21541 gtcaaagctg atcgttgctt gattctggaa aatactattt aaaaacggtg tgacttcttg
    21601 tccgtctacc ttcatattaa tcaaaaactg ctggaaactt tctaaatgga gaacgatcac
    21661 atttttccct ttagcgatcc caaatgtttc gggatttggc tctgcataat gtttctttgt
    21721 ataatctaaa acttcatcaa tcccattact gcttgcatgc gcgcgaacac tgctagtcat
    21781 tcctgttttg attccgtcat aaatagtata cgcatcgatt cctagatatt tgacaatata
    21841 tgaacggtca aatgttcgct gcagcaattg tggacggttg gcttcgctca gcgctagatt
    21901 tcctgaaaaa ataagccctg aaagacaggt aacagcgata gccatcggtt tgccgacagg
    21961 gttgcttttg ataggtattt tcttgacctt caaccaaacc agtagcccaa tgaacacagc
    22021 gatatccagc cagtagatga catcatgggg cttcatcaaa gaaaaggagc ttcctgacaa
    22081 tccttgcgac actttcgaaa agcctaatac agatttgact gtaatgaaat ctgtgaattc
    22141 ccgataaaaa ataatattca agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag
    22201 aatagctaac gctggctttg gctttttaat atacaaagaa aggccaaaaa acaataaggt
    22261 agtcgcaatt gggttgatcc acaagataaa gtactggatc gtaccttcca cacctaatga
    22321 aaaatctgca tagtaggcaa tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac
    22381 gattgctaaa cgattttggc acaatttttt aagttgtgtc acgtatatca cttctctcta
    22441 ttcacatttt ttcttatttt gctcctgaaa aatgaaaatt tcctaaaaaa gttgttatct
    22501 taagattttt ttattctttc taaagattat tccgtccact tttcacagca taaaaccgct
    22561 gcgatgtaag cgattaaact aatgaagaga taaaagtctt gtggtgaccg cttcactcca
    22621 cggtattcaa aagccagttt ctcggtattc gtacaaaaaa agaggctgtg acaaaattct
    22681 tgtcgcagct ccttatttct tgatgcagga cagctttttc acaacttctt ccttaaacgg
    22741 tgttctcctg ccaactcctc ggccacaagt cacaatattt ataaaacatg agaagctgtt
    22801 ttttgaaatt gttccttgtg gctcagagtt aaacggcagg ctcaagacct cttgatacac
    22861 ggtgttcaat cagctgaccc tcggtattag attatgtcag gacgctaatt ttacaacctc
    22921 ttggtaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata
    22981 taaggagcta ttttttgaaa ttgctccttg tggctcagag ttaaacggca ggctcaagac
    23041 ctcttgatac acggtgttca atcagctgac cttcggtatt agctcaaaaa acggcaaaat
    23101 atgaggaact attttgccgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa
    23161 cctctgtttt tcattgtcca taggattgat attgtccttg tgatgactca ttcgtaataa
    23221 ttgaggcttg attttcttca cttcccgtta tcgtttgtcc actgccagaa tctgaaggac
    23281 ttgttcctgt cgactcgttt ttttgagaag agacagttgt tttcttgact tgttcatttg
    23341 tctttttcca ttccgctaaa cgatcggcga ccactttttt gtctactttt tcagaataag
    23401 cggtgatcac ttttccattt ttcaaacggg ctactttgtc gccatcatga gtcaaaagca
    23461 aaacggtcaa atgcttgtct ttaaattgat attgtctgag agaaatcgca ctaagtacgt
    23521 catcgatatt aataaattga ttttcttctt ttccttttgg caaataaatg tccaattcgc
    23581 ctttttcgta attggcagtt attttatact ccgcccacca ataagttgag aattcttttt
    23641 tgaaattatc tactgattct ttgcttgcta cgattttttc tgtttctttc tgagcaagat
    23701 ctgctgtcat ttttcctgaa aatgctgcgg ataaaataag cagtaagaaa aaaccgaata
    23761 accaaaagag aagaaacggt ctttttttcc tcatgctttc ctccactcct atttaatttt
    23821 gactattcta ttatatacaa aaaatgaaat agaaagacaa aaaagttgaa aaagatcgta
    23881 aataattgaa cttctttttc acaaaatggt aagaatggag aatttcacgc atttgctctc
    23941 aactattctt tcttaagcct caaatccgtt tttatctgat acgtctttga accaatgacc
    24001 cgattttttg attgttcttt ttccatcttg tgctaagtct actgaaataa agccatatcg
    24061 attcttatat gcattggtcc acgaccagtt atccatacat gtccacatat gatagcctaa
    24121 acaattggca ccttcttgaa tggcttgatg gacatatttc agatgagatt ggacaaattc
    24181 gatccgataa tcatcgtgga tcatgccatc ttctttgata aatcgtgctt cgttttctac
    24241 acccataccg ttttcagaaa taaagcagtc gatatttcca taatgatctc gtacattgat
    24301 caaaatatca tagattcctt gttcatagat ctcccatccg cgataaggat tcattttacg
    24361 accaggcatt tcataattgt cgaaataatc ctcaggcatt ggaccgtttt tagtatcgat
    24421 tggtgtttct tttgctttta cccggcgtgg ctggtaataa ttgataccta aaagatctac
    24481 tgtattttct ttgataaccg tcaaatcctc tgcatggatc acaggcatga gatcaagttc
    24541 ttttactatc tcaatcaatt cacttggaaa ctcgcctttt acagctggat caaggaatga
    24601 acgattgaag aacgcatctg ctatttgcgc tgctttaaca tcttcagggt ttgattcgtc
    24661 tcttggatag cttggtgtca gattcaagat gataccaata acaccacctt gctccatttc
    24721 atggtaagat ttgatggcta acgctgaagc caacgcttca tgatagccaa cttggatcgc
    24781 ttttttcata tctgactctt ctggataatg ccatcgatat aaataacctg cttcgactgg
    24841 aacgattggt tcattatgag taaaccattt ttttactttc gaaccaaaca attcaaaaca
    24901 acttttggca tatttttcat aggcttcaac cgtttctcgg ttcaaccatc cgcctttttc
    24961 ttgcagatac atcggcatat cgaaatgata cagattcaca aagggttcga taccgttttc
    25021 aatcaactca tcaaaataac gatgatagaa acgaacggct tcttcgttga ttgttccaga
    25081 ttcatcaggt atcaaacggc tccattggat agatgttcga aatgaattat gcccagtctg
    25141 tttcatcaac tgtacatcct cttgatactt cgtataaact tgcgaggttt tttcaggtcc
    25201 tacttgatca aagaactttt gcggttcttt tttgtaccaa taatcaaaaa tgttctctcc
    25261 ttttccatcg cctgaaatcc ttccttctgt ttgtgggcca cttgctgcag aaccccacca
    25321 aaaatttgct ggaaattgat attttttcat caaagcacct ctttttcttt tttagttcgt
    25381 tttagttgat tccatgtatg atattttcca tcacaatcac tttttcattt tcttcctctt
    25441 cttttttgat tcgttttaac agctgttctg ctgctcgttc cccgatttta tctgttggtg
    25501 gcgtgatcgt gattttcgga aggatactca gatcgttttt atctaaataa tcatagtgac
    25561 tgaacgcaat atccttcccg attctgacac cttcttgatt gaaacattcg atggcaccaa
    25621 tcgacatatt gtaattgctg acaaaaatag cagttgcccc tttttctaat aatgcttttg
    25681 ccccgtaata gccgcttttt ctagaatagt ctcctgtaaa gacctctaat tggatcgatg
    25741 gatcattttg gaccgcttct ttggctcctg ctaaacgttc tcttgctacg tagtctgttt
    25801 gagtggcagc aataaatccg atttttttat gtccttgttc accatgatga gcgatcactt
    25861 ttgctacact ttctcgatca ttcactaata ttgtatcaat acctgctggt ccttctggac
    25921 aattcaatgc gataactgga atactcattt ttgtgagcat ttcaaatcca gaccactctt
    25981 cttcggataa gaagacaatc agcccatcga tcacatgctt catcaggtat tcaatttttt
    26041 gttcaatttt ttctggctga tcggcaaacc cgcttaataa taagctatac ccattttctt
    26101 cagctactct ttcgatccct gagacaactt ccgacccaaa acggctggaa atcccgttta
    26161 tcaaaagtcc aatagagaag cttcgattat tcttcaaccc tttagcaatg atgttttctt
    26221 tatagtccaa cgctgaaatc gcttctgcta tttttttcat gttcttttcc ttcagttgct
    26281 gaccattcaa gtaacgggaa actgttccca ctgaaacacc agccatctta gccacatcac
    26341 ggatcgatgc catacattct cactcctgcc agttacattt gatttctctt tcttgttgtt
    26401 cgattatgat gaccgcattc tttgcatagt gttgcttgtc atcaatatcc atataactaa
    26461 agtcttcagt agagcaactt cctctcacta ttaattatac ttaaacaagt atttgaaccg
    26521 gttcattttt aatatagcat ttttttaaaa gattgcaaca cttttttaaa acgctttctg
    26581 aaagtggcgg attttttgcc tttttaaaat tttattctaa aatttactct atttgtgggt
    26641 gttttgtgcg tttttctctt ccatccgttt gaagttttac attccaatgg tataatttct
    26701 ttatcctatc aaatggggga agtattttga agaaattaat cgtactattt attttgtctg
    26761 cgggactttt gacattgact ggctgtgatt tgtttggcgg ctctgaccaa tcctcggacg
    26821 aaacaaccga ttcttctgtc ttaaaagcgg aaaaagcaga taccaccaac agtgtatata
    26881 cgctaaaagt acgtaaagat atggatttat ccgcgggaca gttgacattt acagataaaa
    26941 aaatggaatg gacacgcacg tatcagacac aaggaactga ttcttctgaa tcatctgaag
    27001 aaaaaacagt gctgacagat atcaagatca ctaccaaaga tgacgaatat attatttcag
    27061 gaaaagaaaa taaaaaagaa acaacagtca tatttacaaa aatcggtaat taccgtttga
    27121 aggatgagga tggcaacatc tacagtctat agtgccgtct gttggatctt cttttgtttc
    27181 tccccttctt ttctgctata gtaaagaagc aagatgattg taaaggagac gaaatggatg
    27241 atcgatagtc ctgattattg tacatgtaat aacaacaaaa atatccatac agaatttaat
    27301 gaccaaggaa gcttgagcat atgcgacaac tgtcacaaac tagtcaaaga ttcatttaaa
    27361 acatttgaat cttatcctgt tcaaaattat gtaaaaagta gaagaagata aacaaatcca
    27421 agcaaaactc actatggctg atttttatga acttcaaact cagcttcttc c
//
LOCUS       DARDHA010000020        24766 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000020 DARDHA010000000
VERSION     DARDHA010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.020"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            287..835
                     /gene="aac(6')"
                     /locus_tag="SDA10_000717"
     CDS             287..835
                     /gene="aac(6')"
                     /locus_tag="SDA10_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEG4239647.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1381..3042
                     /locus_tag="SDA10_000718"
     CDS             1381..3042
                     /locus_tag="SDA10_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG4239648.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(3112..3459)
                     /locus_tag="SDA10_000719"
     CDS             complement(3112..3459)
                     /locus_tag="SDA10_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HEG4239649.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            3543..4484
                     /locus_tag="SDA10_000720"
     CDS             3543..4484
                     /locus_tag="SDA10_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4239650.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            4485..5528
                     /locus_tag="SDA10_000721"
     CDS             4485..5528
                     /locus_tag="SDA10_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4239651.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            5534..6604
                     /locus_tag="SDA10_000722"
     CDS             5534..6604
                     /locus_tag="SDA10_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4239652.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            6594..7547
                     /locus_tag="SDA10_000723"
     CDS             6594..7547
                     /locus_tag="SDA10_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4239653.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            7559..8569
                     /locus_tag="SDA10_000724"
     CDS             7559..8569
                     /locus_tag="SDA10_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4239654.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(8854..9675)
                     /locus_tag="SDA10_000725"
     CDS             complement(8854..9675)
                     /locus_tag="SDA10_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG4239655.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(9772..10050)
                     /locus_tag="SDA10_000726"
     CDS             complement(9772..10050)
                     /locus_tag="SDA10_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239656.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            10318..12327
                     /gene="metG"
                     /locus_tag="SDA10_000727"
     CDS             10318..12327
                     /gene="metG"
                     /locus_tag="SDA10_000727"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEG4239657.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(12806..13393)
                     /locus_tag="SDA10_000728"
     CDS             complement(12806..13393)
                     /locus_tag="SDA10_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239658.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            13568..14890
                     /locus_tag="SDA10_000729"
     CDS             13568..14890
                     /locus_tag="SDA10_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4239659.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            15045..15821
                     /locus_tag="SDA10_000730"
     CDS             15045..15821
                     /locus_tag="SDA10_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEG4239660.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            15814..16383
                     /gene="rnmV"
                     /locus_tag="SDA10_000731"
     CDS             15814..16383
                     /gene="rnmV"
                     /locus_tag="SDA10_000731"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEG4239661.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            16376..17260
                     /gene="rsmA"
                     /locus_tag="SDA10_000732"
     CDS             16376..17260
                     /gene="rsmA"
                     /locus_tag="SDA10_000732"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEG4239662.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            17711..18340
                     /locus_tag="SDA10_000733"
     CDS             17711..18340
                     /locus_tag="SDA10_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239663.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(18464..19573)
                     /gene="ugpC"
                     /locus_tag="SDA10_000734"
     CDS             complement(18464..19573)
                     /gene="ugpC"
                     /locus_tag="SDA10_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEG4239664.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            19782..20204
                     /gene="mgsA"
                     /locus_tag="SDA10_000735"
     CDS             19782..20204
                     /gene="mgsA"
                     /locus_tag="SDA10_000735"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEG4239665.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            20527..21063
                     /locus_tag="SDA10_000736"
     CDS             20527..21063
                     /locus_tag="SDA10_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEG4239666.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(21103..22866)
                     /locus_tag="SDA10_000737"
     CDS             complement(21103..22866)
                     /locus_tag="SDA10_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4239667.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(22869..24584)
                     /locus_tag="SDA10_000738"
     CDS             complement(22869..24584)
                     /locus_tag="SDA10_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4239668.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         8018 a         4482 c         5027 g         7239 t
ORIGIN      
        1 gattattata tgaaaacagg ttttatttgt caattcataa ttggtttttt ttaaagcaaa
       61 agataatcta tatcatgatg aacaaacaga gttttttcct ccaattgatg gctttcctga
      121 tctttcctat tttgaataaa gtgtttctgt ataatttttt tattttataa gtaagataaa
      181 taatgaaaga atagagatac tgattggtag ctaaagattt ttgagcgaga acatttcgtt
      241 ttttcaaaag gactgttaga ataagcaaga acaaggggga agacgtatga taatcagtga
      301 atttgaccgt aataatccag tattgaaaga tcagctttct gatttactga gactgacttg
      361 gccggaagaa tatggagaca gctcggcaga agaagtagaa gaaatgatga atccagaacg
      421 aatcgcggta gcagcggtag accaagatga gttagtagga tttattggtg caatccctca
      481 atacggtatc acaggttggg aattgcatcc attagttgta gaaagctccc gacgaaagaa
      541 ccaaataggt actcgattag tcaattactt agaaaaagaa gtagcttcca gaggaggaat
      601 cacgatttat ttaggtacgg atgatttaga ccatggaaca acgttaagtc aaacggacct
      661 gtatgagcat acatttgata aagtggcttc catccagaac cttcgtgaac atccgtatga
      721 attctatgaa aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa atggctggga
      781 caaaccggat atttggatgg caaaaacgat tattcctcga ccaaattctc aataataaaa
      841 agaggttgtg acagaagtaa tcagcttcaa gaaataagaa ggaatttacg aaaattactt
      901 cttgaatttt tgtgaaattc caacttattt ccgaagaagc tacttttgga acaccgtgta
      961 ccagaggttg tgaaaaagtc gcttagtttc gagtagtaag aaaaattttg tgaaaatggc
     1021 tttctacatt tttgcataaa tttggcttaa taccgagaaa ctggcttttg aacaccgttt
     1081 attcagataa gggactgtga caagactttt gtcacagttt ctttttgttt attctttttt
     1141 atctttgtat ggcaataata gacaatataa cgaaaattat ttaatggctt ttggaagacc
     1201 atggaaaacc ttgtacaaag ctattttttc tgaaaaattc cttgaaacct cacgaaaaaa
     1261 tcattatttt atgaaaaaga caaaaaaaag tgttgatttt ttaataatta ttcattaaac
     1321 tcttaaacaa cttcagaaaa tattaattat tctgaaaatt ccaataaagg ggtacgtaaa
     1381 atgaaaaaga aatttacatt tggtttggtt gcggtatgcg gccttgtact tgctggatgt
     1441 tatggcggca gttcagccac aagcagtagt tcagcaaaaa gcggttcagc agatggagga
     1501 ggcgtattta acttagtcgt gcctcaagaa atgcctacag ctgacttatc agttgcaaca
     1561 gatacaatca gttttacagc attgaataac gtttatgaag gaatttaccg tcttgatgaa
     1621 gacagcaaac cacagccggc aggagctagc gagttggcag aagtcagtga agacggatta
     1681 acttacaaaa tcaaattacg agaagacgca aaatggtcaa atggtgatcc tgttacagca
     1741 gcggattacg tttatggctg gcaacgaaca gtagacccag ctacagcttc tgaatatgct
     1801 tacctgtatg cgccagttga aaatgcagaa gacattacag ctggaaagaa agataaatca
     1861 gaactaggga tcaaagcagt tggtgactat gaattagaaa tcaaattgac gaaacaaaca
     1921 ccatatttcc aatatctgct tgctttccca tcatttttcc cacaaaaaca atccgtagtg
     1981 gaagaaaatg gggatgccta tgcatctgca agtgataaag ctgtttataa tggaccattc
     2041 acattagaag attttgatgg accaggaaca gatacagaat ggacttataa gaaaaatgat
     2101 cattactggg acaaagatac agtaaaactt tctgaaatca aagtcagtgt agtaaaagaa
     2161 tcttctactg cattgaactt attcaaagat ggccaagcag atgacgtgat cttgtctggt
     2221 gagttagcac agcaaaacgc aaacgatcca gcttatactt ctgttaaaga agcgcggaca
     2281 agctatatcg aattcaatca acgtgaaaag aattcaccat tcaacaatgt gaacctaaga
     2341 aaagctattt cttattctat cgatcgtgaa gctttagtaa aacaagtact aggtgacgga
     2401 tcagttgtct ctacaggatt gatcccatca gatatggtaa aaagtccaac aacgaacgaa
     2461 gactttgcaa aagaagctgg aaaattagtt tcttatgaca aagataaagc aaaagaatat
     2521 tgggaaaaag cgaaaaaaga attgggcgtt gattctttag aatttgattt gatggcttct
     2581 gatgatgatt catcgaaaaa agtaatcgaa tatgttcaaa actcaatcca agaaaacttg
     2641 gatggcgtaa cagtcaaacc aacacctgtt ccattctctg tacgtcttga ccgctcaact
     2701 tcgggtgatt ttgacgcagt actaggcgga tgggctgctg actatgcaga cccaagcagc
     2761 tttactgatt tgtttgtcac aggtaattca tacaaccgtg gtcgctggag caatgcagaa
     2821 tatgacgaag cagttaaagc agcttcatca acagacgctg gcgatgaaca agcccgttgg
     2881 gatgatttcc aaaaagcaat caagatcatc tctgatgata tgggtgtcgt tccggtgtac
     2941 caaaaagcag aaggtcacct agtgaatgaa aaaatcaaag gtatcgttca ccatgcggca
     3001 ggcgcttcat gggattacaa atggacatac gttgaagaat aatcgttaat accgtgagca
     3061 tgagaaaaaa cgaggctgcg gcaaatgtcg caacctcgtt ctcatattca cttatcctaa
     3121 caaagaaaaa agcatgaaaa tagcagaaag agcaattagt attccggcaa tgatcagcca
     3181 aaagctgtac ataccgtagc tggcagacga gagcgaggaa aattctggtt ttttctttcg
     3241 gttccttgtt cgtgcaagat ggaccgatcg ctgaaaatga tcgaagaaac ccgaagaaaa
     3301 gacccataaa aaaccgccga taatcagaaa cggcaatgac cataagaata gatcattaga
     3361 taattgaatg agtgtaaagg aatggttgat gagatttttt attaaaataa ccagaatcag
     3421 tagaaaagct actgcgtagg ggatagattt ttttttcatt tgattgctcc tttatttttg
     3481 gttcattatc taataatgta gcgaaacatc tgtcagaagt caaaagctag ggggaaacat
     3541 tagtgaatag ttatatcaaa tatgtattaa aacgtgtctt tttcatgatc atcacgcttt
     3601 ggttaattgc aacaatcacc tttttcttga tgcaattgct gccgggcacg ccatatacga
     3661 atcaggaacg tttgagtcct gaaacaatcg agatgctaaa taaacaagtc ggtttggata
     3721 aaccagttat cgtacaatat gggatttatc tttctaattt gcttcaagga gattttggaa
     3781 tttccttcca atttaaaaac cagccagtcg cacatttgtt agctggaaga atcggaccat
     3841 ccttgcagtt agggttacag gcaattatct ttggaacagt cctagggacg attttaggaa
     3901 cgatctctgc catgaagcaa aatacatggg cagatacatc ttctacactc gtagcgatat
     3961 taggtcgttc cattccaaac tttgtatttg ctgttttatt acaatatatc tttgcaatca
     4021 aattacaagt tttgccaatc gcaaaatggg atggctttgt gtacacaatc ttaccaacaa
     4081 ttgcgttggc catgtcgccg cttgctgact ctgctcggtt tatccgtacg gaaatggtgg
     4141 aagtactgca tagtgattat gtggaattag caaaagcaaa aggattgagc cgttgggaaa
     4201 tcgcctttaa acatggtctt cgaaatagtt tgattccatt gatgacttta ttaggaccgt
     4261 tagctgtagc attgatgacc ggatcactgg tggtagaaaa tatctttgct attcctggta
     4321 tcggggaaca attcgttaag tcaatcacaa caaatgatta tcctacgatc atggctgtaa
     4381 cgatccttta ctcatttatg ctgatttttg ttattttgat cgtggatcta ttgtacggtc
     4441 tagttgatcc aagaattcgt gtttcagaag ggagccgagg ataaatggaa atgataccaa
     4501 agcgttatga aacagttgca gatatccctg cggatgaatt tcttccatta caaaaaaata
     4561 ccgaagaaga acgtgaacag attactgcac cttctttgaa ttttatccaa gattcatggc
     4621 gccgtttaaa gaaaaacaaa gctgccgtta tttcaatggt tttactattg gtcatcattt
     4681 tgatctctgt gatcacaatc tttgtttctc ctcatgatcc aactgcccaa aacgtggctt
     4741 atatcaattt gcctcctcgt attccgggaa tcaatatcga tggattgaat ggaaaagcaa
     4801 tggttggtgg tgaacttgtt gataaatatg ctcaagcaaa cgtcccttca aacgtaaatt
     4861 tctttttagg aacagacggt ttgggacgtg atgtattaag ccgcttattt atgggaatga
     4921 gaatctcgtt gctgatcgca tttattgctg cattactaga tgtaacgatt ggtgtagctt
     4981 acgggttggt ttctggtttg ttaggcggac gtgtcgataa cgtgatgcaa cgttttttgg
     5041 aaatcttatc aggaattcca aacctcgttg tcatgatttt gatgttggtc gtttttgaac
     5101 caggaatttt ttcaatcgtg gctgctatgg ccatcacaaa ctggatacca atggcacgta
     5161 tcgttcgtgc acaaacaatg aaattaaaag atcaagaata tgttttagct ggattgacat
     5221 taggtgaatc aagaatgaaa attgcattca aacatatttt gccgaatatc tctagtgtca
     5281 ttattattca aatgatgttt agtattccat ctgctatttt cttcgaagcc tttttaagct
     5341 ttatcggttt aggtcttacg cctccgtcag cttcattagg aacgatgttg agcgaagggt
     5401 acaagacatt cctgtatctg ccttatctat tatggattcc ggcagtaact ttatcggtta
     5461 tcatgattgg ctttaactta cttgctgatg gattgcgtga tgcctttgat cctaaaatga
     5521 aagagtgaag acgatgacga aaattttaga agtgaaagac ttagaaatct cttttgatac
     5581 gtatgctgga aaggtacgag cgattcgcgg tgtcaatttt catttgaata aaggagaaac
     5641 attggcgatc gttggggaat ctggaagtgg aaaatcagtg acgaccagaa gcatcatgcg
     5701 cttgctgtca agcaacgcca atatagatgc tggtcaaatc ctctttaaag gtaacgatat
     5761 cgttggaaaa tcagaaagag agatgcaaaa aatccgtggt aaagaaatag cgatgatttt
     5821 ccaagatcca atgacctctt tagatccgac gatgcccatt gggaagcaag tcgctgagtc
     5881 ccttagaaag cataataaag tttcaaaaaa agagggacaa aaagctgcat tagaattgct
     5941 taaattagtt ggaatacccg aagctgagaa acgtatcaac agttatccgc atcagttctc
     6001 aggaggtcag cgtcaacgga tcgtgatcgc gattgcactg gtatgttatc cggaaatcct
     6061 gattgctgat gaacctacaa cagcgcttga tgtgactatt caagcacaga ttttggagct
     6121 gctgaaagaa attcagacaa aaatcgatac atcaattatc tttatcactc atgaccttgg
     6181 tgtagtagcg aatgtcgctg atcgtgtcgc tgtcatgtat ggaggaagaa tcgttgaagt
     6241 tggaacatcg gaagaaattt tctacaaccc gcaacatccc tatacttggg gactgcttgg
     6301 ttcaatgccg acattagata gtgcgaacga tcgattgtat gctattcctg gttctccacc
     6361 ggatttattg aacccgccaa aaggagacgc attctatcca agaaatgaat tcgcgatgaa
     6421 aatcgatgca gaacaagaac caccattttt tgaactatca aaaacacaca aggcagcaac
     6481 ttggctactt gctccgcagg cacccaaagt gacaccgcca gaagaaatcc aacgtcgctg
     6541 ggctatcttt aaagagaaac aaaaacatac ttacggtcaa ggaggaattg ctgatgccaa
     6601 ataaactact tgaagttaaa ggattgaaac agtatttcaa tgtaggaaaa aagaatgaag
     6661 tacatgctgt aaatgacatc agctttcaca tttatgaagg agaaacattc ggtttagttg
     6721 gagaatctgg aagcggaaaa tccactactg gccgtacgat cattcgcttg aatgaaccta
     6781 ctgatggtga gatattattc gacggtcaag atataacgaa gatcaaagat aaaaaaggtt
     6841 tgacgaaatt ccggcatgat gttcagatga tctttcaaga tccatacgct tcgttgaatc
     6901 ctcgtatgaa agtcagagat atcattgccg aaggaattga tgttaatgga ctagctaaat
     6961 catctgaaga gcgtgcaaaa aaagtcgatg atctattgag aactgttgga ttgaatccaa
     7021 gccacggtac tcgttatcct catgaatttt caggtgggca acgccagcgt attggtattg
     7081 cacgagcatt ggcagtaaaa ccgcgtttta tcatttgtga tgaaccaatt tctgcactag
     7141 atgtgtcgat ccaagcccaa gtggtcaatt tgttgcaaga tctccaaaaa gaacatcaat
     7201 tgacctatct atttattgca catgatttgt cgatggttaa acatattagt gaccgaatcg
     7261 gtgtaatgca taacggcttg ttattagaga tgggaacaag tgatgaaatc tataatcacg
     7321 gtgttcatcc ttatacagaa agtttgttat ctgctattcc tctaccagat ccagaccatg
     7381 agagaaaacg cagaagaatc aaatatcaac ctgaaccaga tgacggacaa gtacgtcaac
     7441 ttcgtgaaat cgcaccagaa cattttgtct atgcaactga gcaggaagtc ccatattacg
     7501 caaaaaaact aaaacgacaa aaggaatctc ttctggttgc taactaggag ggacatagat
     7561 gctaaaagct tttaaattcc gtatgtatcc gacagaagaa caaaaacagc aattgattcg
     7621 tacttttgga tgtgctcgat ttacctataa ccatttgctg aaaaagcggc agaaaagttg
     7681 gcaacaaaca ggagtagcca atttttcgct cacacccgct acattaaaaa aagaataccc
     7741 attcttaaaa gaagtagaca gtcttgcttt agcaaatgca cagcttaatt tggatcgggc
     7801 ttttcgcaat tatttcaagg gacgtgccag cttcccaaag cttaaaacga aaaaaagtat
     7861 gtggcaatct tatacgacca acaaccaaac ccgtacgatc tatctggaaa atggctactt
     7921 aaagttacca aaacaaaaag aactaatcaa gataaatcag catcggccag ttgaaggatc
     7981 gattcgctcc gctacgattt ctgctcgata taatgaagaa ttttatgtcg cactactttg
     8041 tgatgtttcg ccagtaaaaa aagaatcttt agctaaatgg atcgggatcg cctatcatcc
     8101 gaagacattg atccaaacat ctcgacctct agaagtaact ttaccaaaat ttcaccagac
     8161 agaagaaaag ctgcaacatg cacagcggaa attgaatgtg aaagtccgct ccgctcacca
     8221 tagaaagatc aggttagatc aggcaagcaa ttatcagaaa caaaagagaa aagtaatgga
     8281 tctttatctg aaacaaaaga accagcgaga agattatctt gaacaattat ctgggaaatt
     8341 ggtcaaacaa tatgactact tatttgtaga atcttttcca aaagaagagg cacatgctga
     8401 tttttctatt cacgactggc ataaattaat aacaaaattg cgttataagt cccaatggta
     8461 taataaaaaa tttcttttga tcaataccga cggagcagaa gaaagcaatt ccgtaagaaa
     8521 gagccaagta gtagaaaaaa tgggcagaca ttcggttatc aagggataaa aaatgaatga
     8581 aactgatagg agcgttaggg ttagccttgt taataaaaag tcttaatgac ttttgtttct
     8641 aggaattttt cttttgaaga aaagctcact aattatgaca aaccaataaa acaatagcag
     8701 aaaaagttcg tacgagcttt ttctgctatt gttaattttt tattcagaaa gtttacgaat
     8761 acatcaaaat tctgaaacaa aaaacgtaac tcacaaagag ttgtttttgc gttttttctt
     8821 gaatgattag gtctgaatct tttgctcaca accttattca tggtgccccg gcttgagctg
     8881 atagattttt gaaggacggc cgattcctac aggacgttca ccgatctcat cgaaatattg
     8941 gagcatagct ttcttaaaat tggaatgatc gatcgtttta taatcgatcc ctaagaattt
     9001 agcaaatact tttcgagctt ccgtaatcgt gaaatcttta cctaaaactt gtagaacttg
     9061 cggctcgtgt tccattttat tgactacacg gttgaatgct ttcaagatga tttgactatg
     9121 gtcaaaagcc aaagtatctt tgccagaaga ctctccagtg actaaatcaa gcgagatagc
     9181 cacttctccg ctagataaat ggatcttgtt actatgacgt tctagtgtga accagcgtac
     9241 ttccttggca tcatctccgg cgattagcgg ttcttctcca ataaaggcta agtagctgac
     9301 tgtgacgacc cagcctcgcg gatctcgatt aggcgtactg aatgtatgca gttgctcaat
     9361 attttctttt gaaataacca caccagtttc ttcctttgtt tctcttaaga cgctttctcc
     9421 agttgactca tccttttgga caaagccacc aggcaatgcc catgaatttc gataaggatg
     9481 cccttttctt tggatcaaca aaactttcag ctgatcttct tctttgttat agcacatcag
     9541 tacgatatcg acagtcaaag agggcttgtc atattctgga cgttcctgtt tttgatacca
     9601 gttaagaaat tcttcttcac ttgctttatg ttcataatat aatttttctt cttctttaga
     9661 agcaaaacgg ttcatctgac cacctccatt gacagtaaaa gtacttttta ccttattgta
     9721 gcagactctg aaaaaaaacc aaggatttac ttgaaaaatc taggttatca tttaaatatg
     9781 aaaaataaat ggaagtatga gattgaataa aaagaaaagc agataaaacg gcaatacttt
     9841 ataccgctct tcacgaatgg caaagaagcc acaaataacg atatatacgt caatgataaa
     9901 acgcaaaatc caagctgctg tcataaaact gttcaaatgc ccatggaggt taaaataacc
     9961 atacatacga atataaaaat agctgaatat taaatgtgct aatgaaagaa aagcaatact
    10021 aagtgtaaaa aaaagatgtt tcttctgcat gattgttcca gtcccttact aagatagata
    10081 gtctttatta taccacgtgt gaggggagaa ataaaaagcc gttttttagt agatatgaaa
    10141 caaaacaaaa acccgcattt attcaaccta ttcatccttt taaaggatag attgaacgta
    10201 gatagaagtc gtttatccag tctttttcat tttgaaaaaa agctaacagc atgctttttt
    10261 tatggtatac tagcaaggaa acgaattcgc atcatgcgat aaaggaggaa gagccccgtg
    10321 gcagaaaagg aaacttttta tatcactacg ccaatctatt atccaagcgg caaacttcat
    10381 atcggcaact cgtatactac gattgcatgt gacgcaattg ctcgttataa acggttgatg
    10441 ggctttgatg tgttttatct cactggagtg gatgaacatg gtcaaaaaat cgaaacaaaa
    10501 gcagaagaac taggtgtaaa accgcaagaa tatgtagata aaatggcggc tgatgtcaag
    10561 aaattatgga aaacgcttga tatcagttat gataaattca ttcgcacgac agatgattac
    10621 cataaagcag ctgttcaaaa aatatttgat cgtttattag aacagggaga tatttactta
    10681 ggtgaatatg aaggctggta ctctgtttct gacgaagaat tctttacaga aacacaactt
    10741 gcagaagtgt acaaagatga cgaaggtaac gtgatcggtg gtaaagcgcc aagtggacac
    10801 gaagtagaat tggtaaaaga agaatcatac ttcttccgca tgagcaaata cgcagaccgt
    10861 ctattggaat actatgaaga acatcccgaa ttcatccagc cagaatctcg taaaaacgaa
    10921 atgatcaaca actttatcaa accaggatta gaggatcttg cagtttcacg aacaactttt
    10981 acttggggag taccagtcaa gagtgatccg aaacatgtgg tgtatgtatg gatcgatgca
    11041 ttatccaatt atattactgc actaggatat ggctccgaag atgaaagtct attcgaaaaa
    11101 tactggcctg cagatgttca gatggtcggt aaagaaatcg ttcgctttca tacgatctat
    11161 tggccaatca tgctgatggc ccttgatttg cctctaccta aaaaagtatt tgggcatggc
    11221 tggctattga tgaaagacgg aaaaatgtct aaatcaaaag gaaatgtcgt ttatcctgaa
    11281 atgcttgtgg aacgttatgg tttagatgca ctccgttatt atttgcttcg tgctgttccg
    11341 tttgggtcag atggtgtctt tacgccagaa gattttgttt cccgtgtcaa ttatgatctg
    11401 gcaaatgatc tagggaactt attgaatcga acgattgcta tgatcaacaa atattgtgat
    11461 ggactcgttc ctgactatgc ctcacttgtc actccatttg atagtgaact gtctacgacg
    11521 gctgctaacg ttgtcggacg ttaccatgat gcaatggaaa aaatggaatt caatacagca
    11581 ttggctgaaa tctgggtgct gatttctcgt gcaaacaaat acattgatga aacagaacca
    11641 tgggtattag caaaagacga agaaaagaaa gctgaattga atagtgtcat gatccattta
    11701 gctgaatctt tgcgtatcgc agcgatatta cttcagccaa tcatgacaga aacgccagag
    11761 aagatcttca atcaactagg attggactct gaaacaatga atcttgaagg attacacttt
    11821 ggcgaatttc cttcaggaac aaaagtagtt gcaaaaggta caccaatctt cccacgttta
    11881 gatatggaag aagaagttgc ttttattcaa gaaaaaatgt ctgaaggtac tcaaacaaac
    11941 gaagatacag tcaaatggga tccagaagaa acagaacttg tttcaacgaa agaaaaacaa
    12001 atcaaatttg atgtatttga aaaagtagaa ctaaaagtag cagaagtcat caactgtcaa
    12061 aaagtagaag gggcagataa gttgctgcaa ttccgactag atgctggaga cagccaagac
    12121 cgtcaaatct tgtcaggaat cgctgaattt tatcctgatc caagcgaatt gatcgggaaa
    12181 aaagtcgtca ttgtagcaaa tctgaaacca cgaaagatgc gtggacaaat cagtcaagga
    12241 atgatccttt ctgcagaagc accagatggc tctttacaag taatcgaagc accaaaatcc
    12301 atgccaaatg gctcagaaat cgcatagaaa ttcaaagggg gtatgacact agttgtgtca
    12361 tacccccttt ttccgaaatt tgagaagcaa ttttcgtaaa ttccttctta ttacttgaag
    12421 ccaatcatat aatacagaaa cagagaacta tccaaaataa atcctagaaa aagaagtacg
    12481 agcttcgctg atttttgtta atttagaata atactaatag gaatcgtaaa aacagaaaaa
    12541 aagcataatg ataaagggtc gggcagattt taattgattc atctggtact cttttttatt
    12601 gtccttacat tcataaagtt tgaatgcaat gactttatta catctatatt tatatctaac
    12661 agtagcgttg atgtgttaat tataagttct atcactttat gttcaattag tttgtgctct
    12721 tatatgtgtg attgaaaata tagataaaaa aacaatcatc ggctttgact tcgatgattg
    12781 ttttctttat tttgtaaaaa tcctatcact tgattttatg gtaacctttt atttttgtct
    12841 tcaaacggct tggtaagaga gcagtaagca gtgcaaatgg gatcgaaaag acataccaga
    12901 aaatcgtacg tccgatacca taatgttgat agagctttgt caacatcacg tgtaacaaga
    12961 tcaaccctaa aagcaaggag ccagctataa accaatgatt atcccaatgc atgacttcac
    13021 taagtgccat cactccaaaa ggatagaaat aaaaatagat cccgactaag gtcatccgcc
    13081 agttctttgt aaatgttccg atattcaata aaaagaaaat cgctgaaagc aaaataccga
    13141 taggcggaaa aagcccaacc aaaaaggaat acaacatccc ccacaagatc atcacaggac
    13201 ctttgatcaa tgcaacgatt gcgatgaaca aagtcagcaa tattagtttt tgtgataacg
    13261 ttgcaacagt cattaatcca aataaaatta atgtgaacca atgccaatgg gactgctgtt
    13321 gttctttacg ggaaaaatcg acccggttgg ataaataatc tgctaatttc ggataagaac
    13381 gcttaaaatc cataaactgt tcctccttcg tggaaaaggt aggtttagta tacgaaaaat
    13441 aaaaaagaaa tacatccgtc ttaagaggga gtttttcact gaaagtggag aaaaaagaat
    13501 ggtaaattaa aatattctca gttatactga aaattatacc aaatttcaaa gaagaaagag
    13561 gatgggaatg aaaggcaaac taaaacgaga aatcaatctg tttggcgcat tagctaccgt
    13621 tatgggaacc gtgatcggtg caggcgtctt cttcaaaact gctgctgtta ctgctagtac
    13681 tcagtctaca agtttgacat tgctagcttg gcttttgggt ggtttcttaa cgatctgtgc
    13741 tggattgaca gtggctgaat tagctacagc aatcccggaa actggtggag ctgtaaaata
    13801 tattgaagct gcgtatggaa aattgcccag ctttttatta ggatgggcgc aaagcttgat
    13861 ttactttcct gctaatattg ctgcattatc gattatcttt gcgacacaat tgacgaactt
    13921 attacaatta tctaccgatt atcttttgtt gattgcagtg atcaccgctg tttctgtgac
    13981 agggttgaat ttgttaggga caaaagtcgg tgcttctgta caatcagtga cgttgattgt
    14041 taaattaata ccaattgctg tgattgtcat atggggatta ttgaccccag gacaaggaac
    14101 tgtccagtta tttccaatcg aagctggaaa agatgttact tttgtcgaag gattgagcag
    14161 tgcgttgtta gcaacgttgt ttgcttatga cggctggtta ggtgtcggtg caatggcagg
    14221 ggaaatgaaa cggccggaaa aagatttgcc taaagcaatt attcttggtt tgagttttgt
    14281 aacagtcgtt tatctattga tcaattttgt ttttttgaaa actttgccga ttgatcatct
    14341 tgccggtaat ttgaatgcag cttctgaagc atcagacgtc atttttggag gaatcggtgg
    14401 aaagctggtc actattggga tcttgatctc ggtatacggt gcattgaacg gttatacact
    14461 aactgggatc cgcgtgcctt atgcgatggc tttagaagac gagctacctt tcagtaaaca
    14521 gctgacaaag ctttcaaaga agtttacggt tccttatgtt cctgcggttt tccagttagc
    14581 agtagcttgt attatgatga gccttggttc tttcgacttt ttaacagaca tgttgatttt
    14641 tgtgatgtgg ctattcagtt tactgatttt tatcggtgtc tttattttaa gaaaaaaagc
    14701 gccggaattg ccacgtccat ataaagtacc actttatcca atcgttccga ttattgcgat
    14761 tctcggtgct attttcattt taggtatgac gatgatgacg caaacgaaat tagcattgat
    14821 cggtataggt gttaccttga taggtatccc agtctattat caaaaaaaga aaaaacgctc
    14881 agaagagtaa aatatttcaa caacagacag aaaaagtacg aactaggtcc tctagatctt
    14941 cgtgcttttt ctatttcaat aaaacaagtt cttctatttt gatttttgct acttctgcaa
    15001 gtagtgctta tgtgctaaag taagaatgaa aaggagagat aaacatgata tttgattctc
    15061 atacccattt aaatgctgaa cagttcaatg aagatattcc agaaacgatt cagcgagcac
    15121 aagaaatggg ggtaacgaaa atggcggtcg tgggcttcga tacgccaacg attgaaaagt
    15181 ctttgcaatt aagccatgac tatccaaata tatacagtat tatcggctgg catccgacag
    15241 aggctggcag ttatacgaaa gaaatcgaaa ataaattaca agaacaattg actttaccaa
    15301 aggttgtagc attaggggaa attggcttgg attatcattg gatggaagac ccgaaagaag
    15361 tccaagataa agtatttcgc agacagattg ctatcgcaaa agaaatgaac ctaccaatca
    15421 gtattcatac tagagaagca cttgaagaca cgtatcaaat cctaaaagaa gaagatgttc
    15481 gagatatcgg tggaatcatg catagtttca gtggtgatta tgaatgggca aaacgatttc
    15541 ttgacttagg gatgcatatt tcatttagcg gtgtcgtcac tttcaaaaaa gcattagacg
    15601 tccaagaggc tgcagcaaat gtaccaatcg aacgattact tgtggaaaca gatgcccctt
    15661 atctggcacc agttcctttt agaggaaagc gaaatgaacc aggctatact cgttatacag
    15721 tagaaaagat tgctgaactt cggaatcttc cgacagaaga agtggctgcc caaacatgga
    15781 caaatgcgca ccgattattt ggtctaagcg aacatggata agatgaagat ccaagagatt
    15841 atagtcgttg aaggaaaaga cgatacaaga aggctgcggg aagtagttga tgcagatacg
    15901 atagaaacaa tagggtctgc aatcaatgaa gagatcctga tgcaaattga acatgcccaa
    15961 gagactcgtg gagtgatcgt ttttaccgat cctgattttt caggagaaaa aataagaaaa
    16021 atcattatgg aagctgttcc tcaggcaaaa catgcttttt tgccgcgcac acaagctaaa
    16081 ccaaagaaaa agggaagcct tggtgtcgag catgcaagtg atgctgctat tttagaagca
    16141 ttgaaaaagg tcgtgacgcc agacatttcc caaacggctg tttctgaaat cactagacaa
    16201 gaccttttag actatggttt aatagctggg gcacaagcaa aagaaaaacg agaaaaacta
    16261 ggtgacgagt tgcgcatcgg ttacacgaac ggcaaacaat tggaaaaacg tttgaagatg
    16321 tttcgtatta cgccgaaaga actcgcccaa gcaatgaaaa tagtggagga acaacatgag
    16381 tgaatatcgt gaaattgcga caccctctag aacaaaagag attttattga aacatggttt
    16441 ttcatttaaa aaaagtttag gacaaaattt tctgactgaa ccaaatattt taaggaaaat
    16501 cgtagaaact gcgcaaatag atgaacatac gaacgtgatc gaagttggac caggaatcgg
    16561 tgcattgacg gaacaattag ccaagcatgc aaaacaagtc gtagcatttg agatcgatga
    16621 tcgtttgatc cctgtattgg ctgatacgat gcaaccctat gacaatgtga ctattgtaca
    16681 tcaagatatt ttaaagaccg atttgtcaac agctgttaga gaaacatttc atgaggagct
    16741 tccgttaaaa gtcgtagcga atttacctta ctatattacg acaccgatca tgatgcattt
    16801 tcttgaatca gatttggttg tggatgaact agtcgtcatg atgcaaaaag aagtagctga
    16861 tcgtatctct gctgaaccag gaacgaaagc ttatggttca ttatcgattg ctgttcaata
    16921 ttatatggaa gccagtttag cattcatcgt accaaaaaca gtatttgtcc ctcagccaaa
    16981 tgtagattca gcgattttga aactgacaag aagagatata ccggcagtcg aagtgacaga
    17041 cgaaaaagca tttttccgat tgacaaaagc ggcatttcag caaagaagaa aaacgctttg
    17101 gaataacttg cagcattcgt atggcaaaga tgaccagaca aaagcatggt tagcaaaaag
    17161 cttggaaaca gctggaatcg atccaaaacg tcgtggggag actttgtcat tacaagaatt
    17221 cgccgcatta agcaatgcga tgtcagaaaa tatgcagtaa aaaaagaggt tgtgacagaa
    17281 gttgtgaaaa agctgtcctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
    17341 tgtttttcgt ttgtttgggc ttgttaccgc agatgcaagc ttttgaacac cgtttaagaa
    17401 agaggttgtg ggcctgccgt ttaactctaa gtcacaagga acaatttcaa aaaacagctt
    17461 ctcatgtttt tgagttagtt ggtcttatta cttgatgcaa gcttttgacc actattatgc
    17521 cgaacaagga gatgtgacac aaattttgtc acatctccta tttttgtttc ttgatcaaaa
    17581 aattcttata cttgatactc acactcattt tgtatggatt tagaaaagaa tatgtgtctt
    17641 gacagttatt agtaagtgag ttatactaag tcgacattta tgtgtcaaga catattagga
    17701 gagagaaaaa atgagaaaaa attcagttcg aaaactaacg atttctgctt tattgattgg
    17761 gatgggggtc attatcccaa tggtcatgcc taaaatcatg attccaccag cttcctttac
    17821 tttggcgtct catgtgccat tatttattgc gatgtttttt acaccaggag ttgcagtggc
    17881 tgttgcgcta ggtacgacat ttggtttctt tttaacaacg ccggttatca tcgcattgcg
    17941 agcattgtca catcttgtgt ttgcgttgat tggggcttgg tacttgcaaa agcatccgag
    18001 tatcgtttta aaaaatggac agtttacttt tgctaattgg cgtttccaag ggttcaattt
    18061 tgtgatcggt gtgattcatt ctttagcaga aatgctagta gtcagcatct tttactttat
    18121 tggtaatatg cctgaaacat attattctca aggatatttt tatacagtat tcatcttgat
    18181 ggggattggt gggcttatcc acagtttagt tgattataat attgcttatt ttatagcagg
    18241 aacactcagc aaatattttg atatccccgt atttacggca gcaaaaaaag aaaatcggaa
    18301 agagtcgatt caagggtacg cgaagaaaac gacatattag acatgatctc tcaaaaaaaa
    18361 aactctcatc tagtaatgga taataaatcc tctaagatga gagttttttg tcctaaaaag
    18421 agctgcacca acatcttctg gcacagctct aacagggggc aatttatgga agtttgatga
    18481 cttgttctgt ttctttatca aagaagtggc ctttgctgat gttgaaggca aggtcgacta
    18541 tttcgcctgg tttatggaaa tctcttgcat ctacttttga gatgaactca gtagaaccag
    18601 tttttgtgta aagcatcgtt tcagctccaa gtaattcgga aacgacaact tctgatttca
    18661 ctacggcttc aggtgaagct tcaagggcta cttgttcact gtggatgtct tctggacgaa
    18721 taccgaagat aacttctttt ccttcataac ctttttcaac taatactttg ttttttcctt
    18781 caggaatacg aagcttaagc ccatgtccat ctgtgataac accatttttc aatgtcacat
    18841 taaagaagtt catggcagga gatccaataa agccggcaac gaacatattg acgggtgtat
    18901 cataaacttc tttcggtgta ccgatttgtt ggataaatcc gtctttcatg ataacgattc
    18961 ggtcagccat agtcattgct tctgtctgat cgtgagtaac atagatggtc gttgtttcaa
    19021 gacgctggtg aagtttggca atctcagcac gcatggctac acgtaattta gcatctaaat
    19081 ttgataatgg ctcatccatt aagaagactt ttgcatcccg aacgattgcc cgtcccaatg
    19141 ccacacgttg gcgttgtcca ccagataaag cagctggttt tcgtttcaag tattctgtca
    19201 gacctaagat ctctgctgca ttgtccacac gttttttgat gtctgcttta tcatatttac
    19261 gtaatttcaa gccaaaagcc atattatcaa aaacagtcat atgaggatac aatgcgtagt
    19321 tttggaaaac catggcaata tcacggtctt taggtgccac gtcattcatg acttttccgc
    19381 cgatatttag ttctccttca gagatatctt ccagtcccgc gatcattcgc aaagtagttg
    19441 actttccaca accggaagga ccaacaaaaa cgataaattc acggtctttg attgatagat
    19501 taaaatcagt tacagaataa ttttctgcat tgtcatattt tttatatacg tttttcagag
    19561 ccatttctac cataatacgt tccacacttt cttttttagt tttattaact atttaaagta
    19621 taaatgaaag cgttaactaa cttcaatgtc atgttgaaca agaatttttt cttttttttg
    19681 tcaatgtgct agttttaagc tttaataaat atccataaga aataaaaact gatacactgg
    19741 agtaaataag tatgttaaca atatcaaaag gagtgtctct tatgaaaatc gctttgatcg
    19801 ctcatgaccg aaaaaaacct atgatggtga aattagcaac agcgtataaa acaatattgg
    19861 aaaaacatga actttttgct accgggacga ctgggcagag gatcacggaa gcaaccggct
    19921 tgccgatcca tcggtttaaa tccggaccgt taggcgggga tcagcaaatt ggagccatga
    19981 tttcggagga tcagttagat ttggtgattt ttcttcgaga cccgctggct gcacagcccc
    20041 atgaaccaga tgtaacagca cttatccgtt tatctgatgt ctatgagatt ccgttagcta
    20101 cgaatatcgg aacagctgag gtgctactaa gaggactgga agccggcttc ctagattttc
    20161 gaagaatcgt ccatgaaata gacaagcatc cactttcatt ttgatggata agcgtatgga
    20221 aaaataacgt ttaatgttcg cctatatgta gctataaaag ctaaacaaac tgataatatg
    20281 cgatataata cgaacgtatt ggataatata aacaataata ttagtttttc gtatttgcag
    20341 taaatgtgaa aatatgctat agtttctgtg ggaaaagatc ccaaaaataa tacagagtag
    20401 aaaataaagc gttaagtgct aagaggatgg gatgttgcct cttggacgaa ggacgtaatg
    20461 ttcgcggttt tattttcgca ttcgctgcat gatacgatgt agcagctctt aaaggagatg
    20521 ctaaaaatga agaaacaccg gttagacgca cgtatgattg ctattatggg cttattgatt
    20581 gcattgatgg tcactttgtc ccgactggta gcaatcgaga ccccatttat taaaatcagt
    20641 gtcacattta ttccacaagt gatcatgggc attctttttg gtcctttttg gtcagggata
    20701 ggcgcagttt tagctgattt agtaggaatg gccctcttct cgaaatctgc attctttatt
    20761 ggatttacat tgaatgcatt tattgaagga gcaatctacg gattcttttt ctatcgaaaa
    20821 gagatcactt ggaaaaatgc cattctggct acgcttagtg tcacgctgat catcaactta
    20881 tttttgacac cattatggct tgcgttgatg taccatgtgc cattgttcag ctgggtcgtt
    20941 tgggcgcccc gcctgttaaa aacagtaata tggttaccga ttcaatcaat tgccatttat
    21001 tacgtaggac gtagtattcc ttacaaaaaa attttaagaa gtttagcgat ccacgctaaa
    21061 taaaaaacaa gcagtcttcc catcccagaa gactgcttgt ttttattgtg ctgtgaattg
    21121 tgcttgatag agccgtcggt aataaccatc tttttgaagc agctcatcat gggtgcccat
    21181 ttcgacaata gctccctgat ccatcactaa tatctggtcc gcatttttga ttgtggacag
    21241 gcgatgggca atcacaaaac tggtttttcc ttccatcatt gctaagaagg cttgttggat
    21301 ttttttctct gtcaatgtat cgactgagct ggtagcttca tctaagatca acataggggg
    21361 attactgatc atcgttcgag caatcgtcaa cagttggcgt tgtccatctg atatctttaa
    21421 cccttgaccg ccaatcttag tatctaattt ttccggcaaa cgttctacaa attcatacat
    21481 ataagaggct tttagggcat cattgatttc tgcgtcactt gcttccggat tcccaaaacg
    21541 aagattttct cgcaatgtac tgtcaaatag ccaagtatct tgtaacacca ttccaaaact
    21601 tttacgtaat gtatcacgtt ggatagagcg gatgtcatgg tcatcgactg taattgttcc
    21661 ttttgttgtg tcataaaaac gcatcagcaa attaacaagt gtggattttc cggcccctgt
    21721 ttttcctact atagcaatgg tttgacctgg ataagcagta aaattgaaat cttcgatcaa
    21781 acgctgcttg gggtcataag aaaaagaaac atgatcaaaa gagacttttc ctttgatatt
    21841 tttgggttcc aaggcatagg catctggcaa atcaggcttt tcttctggct gatcgatcaa
    21901 atcaaatgct cgttctagac cagaaaaagc ggtttgcagt tgtgtcgtga ttccagaaag
    21961 ttcgataaat ggtttagaaa actgacttga gtagatcgta aagctggaaa tcacaccaac
    22021 agtgattgac gaaccactct tcaatgccaa taggccgcca actaaaccaa tcgataaata
    22081 agcaaggtga tctacaaaac gagaagacgg gtttgtcaag gaagaagaaa attgtgcccg
    22141 ttgtcctttt tcatatagat cagcatttag cagttcaaat tgctgttgac tagcttcttc
    22201 ttgctgaaaa gctttcacga ttttctggtt accgattcgt tcggtcacaa agcccgagat
    22261 atccccaata atcctttgct gtctatcgaa atttgtttgt gaagcacgag caacgatcca
    22321 actcacaagg aaaataattg gtgtagaaca aagaacgacc aatgtcagaa gtggacttag
    22381 acgcatcata aaaataaacg aaagaatgat gattgccact ccagaaaata attgattgaa
    22441 ggccgcagaa attgcaatgg aaatattgtc catatcattt gtaaatcgac tgacgatatt
    22501 tccgtgggag ttttgatcat aataacgaag tggcaatcga ttcagatgag caaatgcatc
    22561 ttttcttaat tttgcaacgg ataagtaagc taaccgattg cttaacactt ggatgagcca
    22621 ttgactgact acgttgatta aaacgatacc accgaataga ataagtaaat ggaacagtcg
    22681 attgaagcgg acctgtcctt ctccaatcat ttgatcgata ctttgaccaa tttgtaacgt
    22741 catcacaact gttgcaatcc cattgatgat tcctagtatc acagctagcc aaatgtcttt
    22801 tgcataggca gtaaggtaag gaatgaatcg gcggagcgtc tgcaaggata atttttttac
    22861 tttcataact attcttcctc ctgtgatgcg acgatttcct gatattctgc tgattgagca
    22921 agtaattctt catgggtacc ctgagcagca atcttacctt gatccatcac taagatatga
    22981 tgtgcttctt gaatagagcg gacacgttgg gagataataa tcactgtact gccaagataa
    23041 gtctgtagcg ccttacgcaa atccaagtct gtttgataat ccaacgcact taaagagtca
    23101 tctaaaatca gtaatgctgg cttggcaatc aaagctcttg caatcgtcag tcgctgtttt
    23161 tgtccgccag agaaattttt cccattttcc atgacttttg tatctaaacc atttggaagc
    23221 tgacggacaa attcttctgc ctgagcgatt ttcagagctt cccaacattc tttttctgtg
    23281 gcgtctttct taccccattg aaggttttca cgaatagtcc cggtaaatag tacagaagtt
    23341 tggggaacaa gagcaatttg ctgacggaga tgatccagct gccaagaacg aacatccatt
    23401 ccgtccatta taactgtacc ggaactgaca tcatagaaac gcggaatcaa ttgtgtcaaa
    23461 gtcgttttcc cgcttccggt tggaccaatg attccaagga ctgtatttcg cttcagctga
    23521 aatgtaatat tttggagaga aagaccagaa tcaggagtat aacggaagtc aacatggtca
    23581 aaagcgataa ttgcttcaga agaaaaatcg ggagtagtac tgacctcagc ttcaagaatc
    23641 gaattttctg tgtctagcac ctctttcacc cgatttcctg aagcttcggc acgtgtgaag
    23701 atcacgacga ggtttgaaac aacgatcagt gccaaaagca tttgattcat ataattgatc
    23761 aaagcaagaa cttgtccttg ttgcaagcta ccaatattta ctttgatccc gccaactgtc
    23821 agcaaacaga tgatcccgac attcatgatc aaggttgtag caggtgataa tagggcagag
    23881 atattggaaa cacgtaaata attgttcgct agatcatttg tgcttttatc gaacttctct
    23941 gtttcttttt ttgtctgcgc aaatgcgcgg atgacacgaa caccgctaag attctgacta
    24001 acgtgttcat tgagctgatc tagtttttct tgcacttttt ggtagagagg aacggttttt
    24061 ttgatgataa aaaataaaat gattgagaaa ataggcaata atgctaagaa aaacagacct
    24121 acttcccagt cgatcacaaa agccatgacc actgacccga tacttaaaaa cggtgctctg
    24181 atcaataaac gaatgaccat tgctaaagct agctgaagct gattgatgtc gtttgtcatt
    24241 cgggtgatca aggtatctgt tccaaaatga ttcaactcgt tgtgagagaa agtattgatt
    24301 tttttgatta gctgattacg taactctgta ccaaatcctt gtgaagctac tgaagcatag
    24361 tattggcaaa tgatagcaca aatcagacca atcacagaca tagcgaccat ccataagccc
    24421 atttcaatga tttttcctcg gtcattcatt tttagtccat tatcgatcaa ggaagccatc
    24481 attaaaggca ggacaagttc gaagacagct tcaagaaatt tgaagaacgg gcccaaaatg
    24541 atttgcttac gatagtcttt tgcataacgt aataactgaa tcatatgatt cctcctatta
    24601 tttatttgtc cacaattgaa aaaacaagta ctaccacatt gtagcttagg aaatttagaa
    24661 tgaaaagaga acaagccttt tcataaatga aagttttatt atgaagaagc tttagaaatt
    24721 gaatagacta ttttttttaa aaaaaagaaa atgagaaaac atgcca
//
LOCUS       DARDHA010000021        24516 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000021 DARDHA010000000
VERSION     DARDHA010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.021"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(127..975)
                     /locus_tag="SDA10_000739"
     CDS             complement(127..975)
                     /locus_tag="SDA10_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG4239669.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1199..3139)
                     /gene="thrS"
                     /locus_tag="SDA10_000740"
     CDS             complement(1199..3139)
                     /gene="thrS"
                     /locus_tag="SDA10_000740"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEG4239670.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3476..3655)
                     /locus_tag="SDA10_000741"
     CDS             complement(3476..3655)
                     /locus_tag="SDA10_000741"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEG4239671.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3702..5525)
                     /gene="cadA"
                     /locus_tag="SDA10_000742"
     CDS             complement(3702..5525)
                     /gene="cadA"
                     /locus_tag="SDA10_000742"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEG4239672.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5803..8082
                     /locus_tag="SDA10_000743"
     CDS             5803..8082
                     /locus_tag="SDA10_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4239673.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8175..8822)
                     /locus_tag="SDA10_000744"
     CDS             complement(8175..8822)
                     /locus_tag="SDA10_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239674.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(9001..9720)
                     /locus_tag="SDA10_000745"
     CDS             complement(9001..9720)
                     /locus_tag="SDA10_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4239675.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9851..11002)
                     /locus_tag="SDA10_000746"
     CDS             complement(9851..11002)
                     /locus_tag="SDA10_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEG4239676.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11219..11959)
                     /locus_tag="SDA10_000747"
     CDS             complement(11219..11959)
                     /locus_tag="SDA10_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG4239677.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11963..12286)
                     /gene="rsfS"
                     /locus_tag="SDA10_000748"
     CDS             complement(11963..12286)
                     /gene="rsfS"
                     /locus_tag="SDA10_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEG4239678.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12343..12936)
                     /gene="yqeK"
                     /locus_tag="SDA10_000749"
     CDS             complement(12343..12936)
                     /gene="yqeK"
                     /locus_tag="SDA10_000749"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEG4239679.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12920..13570)
                     /locus_tag="SDA10_000750"
     CDS             complement(12920..13570)
                     /locus_tag="SDA10_000750"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEG4239680.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13580..13891)
                     /gene="yhbY"
                     /locus_tag="SDA10_000751"
     CDS             complement(13580..13891)
                     /gene="yhbY"
                     /locus_tag="SDA10_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEG4239681.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13907..15016)
                     /gene="yqeH"
                     /locus_tag="SDA10_000752"
     CDS             complement(13907..15016)
                     /gene="yqeH"
                     /locus_tag="SDA10_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEG4239682.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(15013..15540)
                     /locus_tag="SDA10_000753"
     CDS             complement(15013..15540)
                     /locus_tag="SDA10_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEG4239683.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15720..16505)
                     /locus_tag="SDA10_000754"
     CDS             complement(15720..16505)
                     /locus_tag="SDA10_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEG4239684.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16498..17367)
                     /locus_tag="SDA10_000755"
     CDS             complement(16498..17367)
                     /locus_tag="SDA10_000755"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEG4239685.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17368..18747)
                     /locus_tag="SDA10_000756"
     CDS             complement(17368..18747)
                     /locus_tag="SDA10_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEG4239686.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18755..19174)
                     /gene="fabZ"
                     /locus_tag="SDA10_000757"
     CDS             complement(18755..19174)
                     /gene="fabZ"
                     /locus_tag="SDA10_000757"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEG4239687.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19199..19675)
                     /gene="accB"
                     /locus_tag="SDA10_000758"
     CDS             complement(19199..19675)
                     /gene="accB"
                     /locus_tag="SDA10_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEG4239688.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19678..20913)
                     /gene="fabF"
                     /locus_tag="SDA10_000759"
     CDS             complement(19678..20913)
                     /gene="fabF"
                     /locus_tag="SDA10_000759"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEG4239689.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20932..21669)
                     /gene="fabG"
                     /locus_tag="SDA10_000760"
     CDS             complement(20932..21669)
                     /gene="fabG"
                     /locus_tag="SDA10_000760"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEG4239690.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21681..22598)
                     /gene="fabD"
                     /locus_tag="SDA10_000761"
     CDS             complement(21681..22598)
                     /gene="fabD"
                     /locus_tag="SDA10_000761"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEG4239691.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22705..22929)
                     /locus_tag="SDA10_000762"
     CDS             complement(22705..22929)
                     /locus_tag="SDA10_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEG4239692.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22982..23944)
                     /locus_tag="SDA10_000763"
     CDS             complement(22982..23944)
                     /locus_tag="SDA10_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEG4239693.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23954..24394)
                     /locus_tag="SDA10_000764"
     CDS             complement(23954..24394)
                     /locus_tag="SDA10_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG4239694.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6716 a         5442 c         4263 g         8095 t
ORIGIN      
        1 ttaaaaaata aacttatttt tgaaaattat gttttcttaa gtcgtgaaac actttgatgg
       61 atcatttatt tgtggaaaac tacgttttgt tattctaaaa aacttatata aaaaatagta
      121 aggtttttat ttcgaatcca taaaatattc ttggattgct tctcgtgttt ttttcacttt
      181 ttcgctaagc ttttcgtttt tagccatata gaaataaaaa atgatatcga tcgtagcaaa
      241 ttgggccaaa agtgaattag ttgccgcact tcgattcgtg atttcttttg gtctagatgt
      301 ttgtacgagt acatcggcta tcttgctaag cgggttattt ccaaattgcg tcaacgcaat
      361 gatttcgata ttcatgcttt tcgccagctc ggctaatgcg actacatctg cacttctacc
      421 tgagtttgaa acaagccaaa gcactttctt tttctcattg ctgacaagtt gcgggagcaa
      481 gacgtggata tctttttcaa aaatgattgg cttaccgaca cgagaccatt tttgcagaat
      541 atcttctact actaaagaag aggcgccaac cccatataaa aaaacagtct ctgccgattc
      601 caaacactct acgacttttt ggatcgtttc ttcttctaga atatctactg tttcttttat
      661 cgctacttgt gcattgttac caagtttgtt tttgatagag taaaaggact catttgcttc
      721 tatatcacta aaaccttcat cgattccagt ctgtagttct gttgaaatcg tgattttgaa
      781 gtccgtcaga ctttgaaaac cgattttttt ggaaaaacgt acaactgtcg gcgcactcaa
      841 cccggaagct tctgctaatt cattagctgt cattgtaggg accttctcaa tattaccgat
      901 aatgtagttg gccaactttt tttctgctga agaataatct tccatcatac tttcaatacg
      961 actaattacg ctcataagtt cctccaccct tttaatgata gtctatgtaa gtataccata
     1021 tcagaccctt attcacggtc gtatactgga taaaaaacaa aaaaacaatc cgcccaacag
     1081 aatatcttat cccatcaatc ccacagattc tattgagcat ctgtgtcttt gaggaatacc
     1141 tgttatctat tgttcggatt gctttctatc tactactttt ttacaggaaa tttacctatt
     1201 agttttctct gctgtaattt ttgacttctg cttccatagc atcaataaag atattcaaat
     1261 cttctacagc tgtttcttta cttccataac gacgtacgtt cactgttgca tcttccaatt
     1321 ccttgtctcc aacaactaat tggtaaggga tcttttgtgt ttgagaggca cgtatcttat
     1381 agcccatttt ttcattacga tcatcaactt ctacacgcaa ccccttcatt tgcaaacgtt
     1441 ctttgatttc ataagcataa tcgccatgtg catcaacaga aacagggatg attgttgctt
     1501 gtataggtgc caaccatgtt ggaaaagcgc ctttgtatac ttctgttaaa taagcgacaa
     1561 aacgttccat tgtagaaaca attccgcggt ggataactac tggacgatgc gtattttccc
     1621 cgtcttctcc aacatatgtc aaatcaaagc gctctggcaa taagaagtct aattgtatag
     1681 ttgataatgt ttcttctgta ccaagagctg ttttgacttg aacatctagt tttggtccgt
     1741 aaaaggctgc ctcgccttct gcttcaaagt aatctagttc taattcatcc atagcggctt
     1801 tcagcattgc ttgggctttt tcccacattg cgtcatcatc aaaatattta tctgtattgt
     1861 ttggatcgcg atagctcaag cggaaacgat aatctgtaat attgaagtca gcatatactg
     1921 ccaccatcaa ttcaagcgta cgtttaaatt catctttgat ttgatcagga cgaacaaatg
     1981 tatgaccatc attcaacgtc atttctcgta cacgttgtaa acctgacaaa gcacctgatt
     2041 tttcatagcg atgcatcatt cctaattcag cgatacgaat tggtaattca cgataagaat
     2101 ggatcgtgtt tttgtagacc atcatgtgat gagggcagtt cattgggcga agaacaagca
     2161 tttcgccatc tcccatatcc attggcggga acatgtcttc ttgataatga tcccagtgtc
     2221 cagatgtttt gtacaactca acatctccca ttattggtgt atatacatgt tggtaaccta
     2281 gactaacttc tttgtctacg atataacgtt cgatcgtgcg tcggattgtt gccccttttg
     2341 gaagccagaa tggcaaacca gaacctactt ctggggaaac catgaacaag tcaagttctt
     2401 ttcctagttt acgatggtct ctttcttttg cttcttcacg gagacggata tattctttca
     2461 aagctttctt gtcaaaaaag gctgtcccgt atacacgttg catcatttga ttgtttgagt
     2521 ttccacgcca ataagcacct gccactgata ataatttaaa tacttgtata cggccagtgg
     2581 atggaacatg tggtccacga cacaaatcga tgaattcgcc ttgttgataa gcagtaatca
     2641 cttcttcttc tggcaattca ctaatcagtt ctactttgta cggatcactt gcaaaaattt
     2701 ctaatgcttc ctgttttgaa agaacacggc gttcgatcgg cagattttct ttgacgattt
     2761 tcatcatttc tgcttcgatt gccggcaaat cttctgcagt tacttggttt tgtccattat
     2821 ctgtatcgta gtaaaaccct gaatcgatag ctggtccaac gccaaagtgg atatttggat
     2881 agaggcgacg catggcttgg gccatcaaat gagctgctga atgacggact aagccaagtg
     2941 catcttcatg gtctggtgtg atgatctcaa tacttgcatc ttcctcgatt ggacgagtca
     3001 aatcaacgag ttttccgttc actttaccag ctaaagcttt ttttgctaag ctttttgaaa
     3061 tgctttcagc aattgccaat gttgttgttc cagactcaaa ttctttgact gcaccatctg
     3121 gaaaagtaat ttttatcata atagtttcct ccttgaaatg ttttgattga tcagatcatc
     3181 aagctgaaaa acaaaaaaat cctagcatct cttgaaaaga gatactagga cgaaaagttc
     3241 gtggttccac ctaatgttca aacgcaaatg cgttcctcaa gcgttgtaac ggtccgaccg
     3301 cccttgcttc aacaagggtt tcgaaagtgg tcatcctttg gttttctcct ggaaaagttt
     3361 cagcctagct tttcctctct tatcgaagac tctcaaattc agttggcttt ctcatcaatc
     3421 aattatgtgt ttatttaacc actaataaat agaaaaatca agtgggaaac taaacttatt
     3481 ttttccattc cccatttaca ccgtatgttc cttttttcat ctcattgatg atgacatgga
     3541 tgtgttcttt tggtgcctgc gtgtttttat gaacagcttc cgtgatatct ttcatcatat
     3601 tttctaattg ttccggactg cgaccttcta ctaattcgac atgtacgaat ggcataatgc
     3661 tctctcctct ttctgatcgt tcttttttca actcgattct atcatactat gtttgtcttg
     3721 ggtactgaat caggaaaaat ggctggatct ttttttgctc tcaaggcact caaaatagag
     3781 accgtatcca cgacttcttg caaaacagct ccgaataatg cggggattag tcccgtactt
     3841 gcaattaaca tcaaaaaaat acaaataaag atgccaatca gtacagactg tttcgctatt
     3901 ctcatcgtat ctcttgaaat cactactgct tttgctacac gcaagagatc atctttcaag
     3961 atgaccgcat ctgcactttc actagcagct gtagaaccat gagcccccat tgcaataccg
     4021 atatctgcag cagcaagtgc tggtgcgtca ttcacgccgt ctcctaccat cgcaacaggt
     4081 ctttgatctt tagggatact ttctaaaatg gtcagtttat cttgaggcaa gctacgggca
     4141 tgtacagaat ctaattggac tttttctgca atcgtttgag caatggcaga tccatccccg
     4201 gtgatcatca tcaaattttt cacatcaagt tttcttagct ccttgattgt ctttgctgct
     4261 tctgggcgaa tctgatccga aaataggatc gttcctgcaa aagatcgatc aattgttaca
     4321 tagattgctg tattggacgt ttctatttct tcagctgaaa caaaagaggc acgtccaatc
     4381 tttacttcat gattattgac tactgcttgg attccttgac cggtcacttc tttcagttga
     4441 ctaacaggat acagttggct gtttttcacg ttgttcacta acgaacgagc taagatatgg
     4501 ctagattcct gttctgcact agctgcatac agaagtagct cttctttttt tattccattg
     4561 actggctgaa tcgcctctac ttccaatatc cctttggtaa gcgttcctgt tttatcaaaa
     4621 gcaatcgttt ttgtacgtgc gagcttttca atcgttgttc cagtttttac aacgatcccg
     4681 tttttacttg aacggctcat tcctgcaact aaagcaatgg gcgcagctag aatcagtgga
     4741 caaggtgatg ccactaccag tacctcagca aatcgtaaag ggtcttttga gaaccaccaa
     4801 gaaataccac cgatcaaata agcaatcaac gtgaaaggga ctgcataacg atcagctaaa
     4861 cggacaaatc gggcaggctt ttcttttgat tcttcgacta gtttaataat catttgatac
     4921 tggctgtctg cggcacgttt ttcagcgatc atttggatcg atccgtctcc atttaaagac
     4981 cctgacataa tctgatctcc aggtttcttg gtttccgggc gggactcacc tgtcaaagaa
     5041 gattcatcga ccgaagattc tcctttggtc actcgaccat ctgcgggtac aagttcgcca
     5101 ggcttgacta gtaactggtc atcaatattt atttcatcga ttactatatc ctgcaatttt
     5161 ccttctttga atcgatgggc aattcttggg gtgttatcta acaaagactg cagttcttta
     5221 cccgctcgtt tattcgcgta atcctctaga ctatctccgc ccgtaagcat gatcaatatc
     5281 atcaaactag cccagtattc tttgacggct aatgttgcaa ggattgcggt aatcgctaat
     5341 acatctacgc catatcttcc tgaacgtaac gtcttgatca tttccaaaaa catcaagaag
     5401 gcgagaatcc ctccagtaaa tgcgacaatc gcaaatgcaa gggccggctg gtgaaaaaca
     5461 aattcactta ataaagctat cccaccaata agcaaagtaa gtaaaaattt tttgaaatgt
     5521 gtcattgttt tttctcctct tttatgtgac ttagagaggt tagtaataaa tttccagctg
     5581 cctctttcat cttaagcata gtccttgact agcacttttt aaaacaatag gccttaattg
     5641 aaaacaacta tcactatcag cgtttccaaa attcatcagt tctacaaaca aaaatattca
     5701 gcaaggatta ctttttcgat tcatcagtta aaaagtacct tttttcgaaa aactatgcta
     5761 tacttttaat tcgttattta cggcggaagg agcaaacatg cgatgaatga gaaacaacta
     5821 gaacaaacgc acgtggatga aacgatcgaa ctaatccaga tcgaacaaaa tatgctggat
     5881 acaaaacaac aagaattgac tcaggaaatg aatcattcga caaaagacac agctaatcac
     5941 aagattcgag ctggttccaa tgaagctttt tatgaatctg ctgttgaata tcgccaacat
     6001 gagcaggaat tgcttttgaa ataccatact cttgaagcac agcaaaaacg cctgcaaacg
     6061 ttgtctgtca tgaaagatag tccttatttt gcccgtatcg actttaaaga agaccgtgaa
     6121 acagaaacat tatatctagg tatcgcttct ttaagagatc tggcagaaga aacaatcgtt
     6181 attgactggc gcgcaccaat tgccaatctt tattatgaag gtgagattgg tcccgcattt
     6241 tatgaaactg aaacagatag aatagatgtc gatctgcagt tgaaacgaca atttaaaatc
     6301 caagaaggac gtattttatc tatggtagat acgtcggaag taatcaatga tgactttttg
     6361 ctagagatat tggatgaatc atcaagcaac catatgaaaa acatcgtctc tacgatacaa
     6421 aaagcacaaa atgcaatcat tcgggacaca acaagtaaag taatgctgat tgaaggaatc
     6481 gccggaagtg gaaagacatc cgctttgctt caacgtattg cttttctgtt gtatcataat
     6541 cgcaagtggc tcgaggctga aaacgttctt ctattttcac ccaaccattt gttttctgat
     6601 tacatctcga ctgtattgcc ttcattaggt gaaagcggcg tgcctacaca gacattcaaa
     6661 aattatttgg tgcagctgct gccagagttt tctttactag atgaacagca acaagaaagt
     6721 ggttttttgc taggggaaaa agatccgata caagtgatga aaagcggatt gaccttagtc
     6781 gatcagattg atcgctatat ccaaggcatc acaaactacg gtcccttgtt ccgtgatatg
     6841 aaaataaatg gccgtacgat cctatcaaag gaatcgattc gccaatggta taaagaaaca
     6901 aatgaacaat tacctttaca tcatagactt tctctattac aaacgaagct cctgaaaaaa
     6961 ttaggcggtc tgcaaaaaga cgaaacacgt cagcaatggg tcaaagatct ggcggaagaa
     7021 cagctccaag aactatacgc aacagatcca aatcttgaat atacagaaca aaaggagcgt
     7081 tcattacgta aaaaactggc caaccaaatc gtacgtaaaa gattccgtcg aatccatcga
     7141 gggatcatga attatcaatt tgtcaatata acgaaacaat atctccactt tctacaaact
     7201 gtgccacttt ctcttctaac acgacataaa atcagcgaga ccaggtggca ggaacatttg
     7261 acaactgttc gtacacactt gcgtcaaaga gaattgcgtc aagaggatgc tgtactattc
     7321 tttttactga tgcgccgaac atatccagtg acggttacac acaaagctcg ctatatcttt
     7381 attgatgaaa tgcaggattt cgcaccatca caagtggcat tgctgcaagc cttgttccca
     7441 aaagcaaatt tgactttctg tggagatcta aatcaaaatg tctttggaaa tgaaacgatc
     7501 gttggatcat tggaagacct tttcgtggac cgtgaagtca ctcgtttcca attaacgaca
     7561 agctatcgct ccacaaaaga aatcactgat tttgctaatc attttattgt gaaagaacat
     7621 caagtagaaa caacagcaag aaaaggagac cttcctttgc tcatccaagg aacaacagat
     7681 gatatcaaat tgaattggtt agcagatacc attcgaacga ctcagggaaa agcccggtat
     7741 tggcgtacag caatcatagc taaaacacaa gaagaatgcg aagctcttta tgctcaactg
     7801 ccagacgagt tgaaacaaca agtccagctg attttaaatg agagtgactt tatgaagcgt
     7861 tccattattt tacttccagc ctttttagca aaaggtctgg agtttgaccg agttttcttg
     7921 tgggatgtag atgcagaaca tttttcttct cagcaagaca aattgatcct ttacaccatg
     7981 tgtacacgtg cgatgcatga attgaccctt tttacctcca ataatgaaag tcctttgtta
     8041 aacaatatgg atatttcatc ttatgagaaa aaagtactgt aagtaaaaag acaactgcta
     8101 tcattaatgt tagcagttgt ctttttattt acaataataa ttgcagaaaa agtaattcaa
     8161 tcatttaagg tcatttattc tatatataaa tccaaatctg tttcaggata ttttaatttg
     8221 acaagcgaaa taatcatttt cagttcttcc atgttttctg gatattctgg gtcaaatacc
     8281 ttgctatgac ggccaatccc gccattgtcg atcaatcggt aaattcgatt ccattcaatg
     8341 aattggtcaa ttgattggaa attgctgatt ggatagccgc cacaatccaa aatctctgat
     8401 acacgaaatg ctaggtatgc actatcgatc actttatttt ttttcgtgtc tatcttctca
     8461 tttctttcga taagaatttt ttcaggacga tgtttcaagc tccactctaa tagtagaaag
     8521 aaagcctcca tccgatactc ttcttcaaat aaccgaaagg ctgaatcaga acgacgaaaa
     8581 agcagcggaa tcagataatt ttgtctataa atcagcattg gtgcgcggtc caccatacac
     8641 tctaaataac ggttcagata ccctgtaccg acatagtttc ctaatcgata gccttcaacg
     8701 ccgaatcttg cgtatttttg cgctaatttt gaacaatcag ttacgttcac agctaactct
     8761 tctactttgc ggtaaccgct taagatacgt tcaaaaggtt ctaaagtgat ttttgcttcc
     8821 atattcttcc ccgtccatct tccacatact tcatatcatt tcacatgata cttatgctct
     8881 catcatacca caggttgaat tagaaaagaa aataaatcac acaaaaaagc aagacaatct
     8941 gttagtgaga ttgtcttgct tttcgaaaaa gactgaatac ttttcgttaa aactggttgc
     9001 ttagtcttct aatgccacat tatggaacac atgctgtaca tcttctagat cttccagtgc
     9061 atcaatcatt ttttcaaatg tttcttgatc ttctggagat agtgttacct cgttttgagg
     9121 gatcatttgg atctcagcga ttgaaaaatc agtgattcct ttttctttta atgcttcttg
     9181 tatactatgg aaatccgcta cttcaccgta aacgatgatt tgtccttctt cttccattac
     9241 atcgcgaaca tcgatgtctt tttccattag gtattcaagg atctcgtcag cgtcttctcc
     9301 tgcaaaagca aagattgctg tattgtcaaa catgtaagaa accgctccgg aaacacccat
     9361 gttcccgcca tttttcccaa atgctgcccg tacgtctgct gctgttcgat tcacattgtt
     9421 cgtcaatgta tcaacaatca ccattgagcc gcttggtcca aatccttcat agcgcaattc
     9481 agagtatgtt tcatctcctg caccttttgc tttttcgatt gctcggtcaa taatatgttt
     9541 tggtacatta taagttttag cgcgatcaat gacgaaacgt agtttttggt ttgcatgcgg
     9601 atcaggatca cctgattttg ctgccgcgta gatttcaatc ccaaattttg cataaatccg
     9661 gctattatta gcatctttag cagttttctt tgctacgata tttgcccatt tacgtcccat
     9721 attttcactt cgctttctga tttgatagtt tcataacgca aaaacgtcct gttccattat
     9781 acttgtgaat cacgattctg tgaagctttt tttctttctt ccttcattct tctcattttt
     9841 agtcaattat ctatactcgg attgggattt gcccgaaatt ctgttccgga atattgcctc
     9901 ccaattgata gatttgatca cttctaacta acagttccgc ttcttgtgcc tctttttttc
     9961 cgattcggga ataaatagaa atctcttttt tttcttttaa gaatcttctt ccagcatcgg
    10021 taaatccaag tagtcgaaga taatttttat tttgttggac aggaatctct tgctttttga
    10081 tattcagcaa gacataacaa gataatcgtt ggatccgtgt ccatgtatat ctttttgttt
    10141 ttacctttga cacaaaatcc tcaaaactat cagcagttgc agctgctgtt ttcatgcgcg
    10201 attctattcc ttcggtcatc tgatagatat ctgtcagttc cagcaaagta gaagataaca
    10261 accgataacg caaatacggc caataagatt cccaagtctg cagcgaacaa gttgaaagat
    10321 cttcttctgt ctgaggcggc acaagtccag ctattctttt tccttcttct aacgcatggc
    10381 gaatggctgt tgcactggct atcctctgat ctgtgatgat tttttcatga tagcctgcac
    10441 ttttacgtcg gatcggatgt aattccatag ggtggacata cttggcattt tcctttgcat
    10501 aacttaatcc caagatatga tttggcgaag aaaaatcaag cgtaatatcg ggatacagac
    10561 tgcgaaaaac ctcagtcatt ttttgcggat aactatacgt ttgatcagac agttcttgaa
    10621 atgcctcttc tatcctatct tgattctctt ggacaaagcg tccgaaactg ctgtaatcaa
    10681 acgaagtctc ttcttctgtt ccaaaacaca aactgtcaca gtccagtgtt tgcaagattt
    10741 ttactgcacc tttagcaaaa tagtcagctg actgaaccgc ccattcgacg ggaagctcca
    10801 ctactaaatc cacaccattt tgtaaagctt cattcgcgcg atgccattta tcgatgatag
    10861 ccggttcgcc tctttgcaaa aaattcccgc tcattacagc aatgacgacc tctgctcctg
    10921 ttatttttcg tgccatttcc acatgatagc gatgtccatt gtgaaaagga ttgtactcta
    10981 cgataatccc gcagcttttc atgcgcatcc tccttcatag ttatcgataa aaaagaaata
    11041 gagacaagta aaaaaaggca aaaatcggaa aaaatacccg aactatataa actattctcg
    11101 caaacacaat acttctgcga cgaaaagctg cactgcaaga gcaatgccta tatcgttcgg
    11161 atatataatt tatgttgaag atttattctt ttcttgttaa ctcgagcagg caaaaccgct
    11221 agtctttgta tgctacaaag aaccagcgct ttgatttctc tgatgctgct tcgtctgtaa
    11281 aatcagaaaa tgcttcgact ttcgaaaagc cgctgttttc cagcattctt aaataacttt
    11341 ccaatggata ggtacgctct tgatgtagtt catcttcacg aataaatcga ccagaccctt
    11401 cttgttcttc aacgaaaaag gtaagaaaat gttcgatgct gtgtttctcg cttcctggat
    11461 aactatccca caaaaaagca aaattatccc cttgataatg atagctatat tctggaaaaa
    11521 cctcatctac ttgataggtg gagtgtacgt caaataaaaa tcggccattt tcttctaacg
    11581 cttgatagac ttcatcaaac acttgctgta cctcttgtct atttttcata tagcacagcg
    11641 aatcagaaaa acaggtaatt gcctgatatt gtccgatttc tgataaatcg agcatattgc
    11701 cttccacaaa ttggatcgat gcgtcttctt catatgctcg ctggctggca atcatcaaca
    11761 tctcttctga aaggtctaac cctgtcactt tatatccatc ttttgcaaag gcaaccgcta
    11821 atgctccagt accgcatgcc agttccaaaa tattttttgt gtcttcttgt aaatgacgtt
    11881 tcgaaaaagc taaccattgc tgataaagtg tttcgtccat cacttcatcg taaatgaaag
    11941 caaatgtttc ataagccata tgttaatcca accattcact aagatcgact agcggagcat
    12001 ctgaccatag tttttccaga ttatagaaat ttcgttctgg cgcatggaaa acatgtacga
    12061 tcacatctcc aagatcgatc aatacccatt ttccgccttc ttttccttca atacgtttta
    12121 cttcatattt gttttcttct tctttatcaa tgatttcttc tgtaattgcg ttgatttgac
    12181 gttcgctatt tgctgaacag atcatgaaat aatctgccaa caaggaaaca tttcgaacgt
    12241 ctaaagcgat aatgtcttct gcccgttttg aatctgctgc ttcaatcgca atttttaatt
    12301 gttctttact atctatgatg atttcctcct aatatgagca gcttattttt ttgctaccca
    12361 gtaattatat gtttcgattg tttttggata aatctgttgt tcttgcgcaa gcaaatgggc
    12421 caatgtatgt tttgtttcaa atgctacagc ttcgtctaga tcagcccatg caatggctcg
    12481 tgcatcttgt accccaggaa aatctcgacc tggttcaata tagtctgcta cataaatgat
    12541 tttgtcaagg agactcatct tagcagcccc agtcgtatgc acccggattg catgaagaat
    12601 ctctgcatcg gtgattccta attcccgctc tacaaacgaa gcgccaacaa gaccatgcca
    12661 gattgcatta ttataattca ataactccgg atcaaaaccg tctcttacga ttaccatttt
    12721 gaactcctca tctggtcgtt cttttgcata atcatgtgtc aatgcagcaa tactagcttt
    12781 ttcaggagaa gcaccgtatt ttttggcaag agccacggct gtttcttcta ctccaagcac
    12841 atgcttgaag cgttgttcgc tcattctcat ctgtattttt tgcatcaggt tttctctaga
    12901 ataagcagta tattcattac tatagtccat attcatacaa cccttcattt tgaatataat
    12961 caattacttt atctggaaca aggtagcgga tagaacagcc ttctttgatc ttttgacgta
    13021 ttttcgtgga actgatgtcg atctcaggta catctaccca aattacagga tatggagtat
    13081 ccgttgtgta tcctggtcga cgtataccca caaagttgac catagaagtc agctcatcga
    13141 ttttatacca ttttggcaaa tattctacca tatccccacc gataataaag taataatctg
    13201 tatccggatt gttttgcgtc aattccttca tagtatcata ggtgtaactt ttcccaccac
    13261 gagctaattc aatcgtttca atctgcaaaa aaggattgtc ttccaccgct aattccaaca
    13321 tgttgagtcg atgttttgca tcaatcgtct gtttctcatc aacatgaggt ggctgataag
    13381 aaggcatcaa aaatacgcgg tcaagaccta aattccggcc agcttgttca gccatcacta
    13441 aatgtgcaag gtgaacggga ttgaatgttc cgcccaagat ccctacttgc tttttcttct
    13501 ctaagaattg aggcatttct tcaggaaata attgtgaacg aacaaatgtc ttagcttgcg
    13561 tattcatcat ggtcactcct tatattgctt ttacttcttt tgatagcctt tgatactttt
    13621 cttttgtgga aggtttgtat aatacaataa cacgtccaat gatttggacg atttcgcaat
    13681 ctatttcttt ttctaatact tgagctgctt cttctgcaat ctcatctgta ttttgaagta
    13741 acgatacttt gatcagttct cttttttcta aagcttcttc aatttgaacg atcattgctg
    13801 aattgatccc accttttccg atttgaaaaa tcggctgaag atgatgcgct ttgctacgta
    13861 aaaaacgctt ttgttttcct cttaaactca taattttcct tcttccttaa atcaatgctt
    13921 tgcgtttcaa tacatctaca ccctttggcg cccagcccgc aattactact ggcgctgtta
    13981 cagtgatcca gcctagaccg gcaaaaacga tgtctgtttt ttctttcaca gaaaactcaa
    14041 aacgaacaag ctccggaaat gcctcgactt catcaggacg aggaggctgt aataatccac
    14101 ctacatgttt ttgataaaat tcgtctgctt tttccagttt cgttcgatga atatttacat
    14161 catttgacac ataagcaata aatgaagcac gattgccgct gataaaatca aatcttgcaa
    14221 gtccgcccaa gaaaagtgtt tgcccttcat tcagctgata aactttaggt ttgatttctt
    14281 ttaacggtgc aatgatccgc aaatcttttt tacctaagta gtgcgccatt tgatggcgat
    14341 ggataattcc cggtgtatca atcaaaaaat gcccgtcatc taaaggaatc tcgattttat
    14401 ccaaggtcgt tcctggaaac tgcgaagttg tgattacatc ttgtactccc gctgtatttt
    14461 tgatgatttg attgataagc gtcgattttc cgacatttgt tactccgacg atatagacat
    14521 cccgtccatc gcggtattct tcgatttttt ctaatagttc cgtcatctca tgagatttct
    14581 tagcactagt taataataca tctactggac gaattcctgc ttcgtaagca cgttccttca
    14641 tccattgtgt taatttagtt tttttcaatg atttaggtaa tatatccact ttgttcccaa
    14701 ctaaaagtac tggattgtca ccgacaaaac ggtgtagtcc tgggattagt gaaccattga
    14761 agtcaaaaat atccaccaca ttgacgatca atgcatcttt ttgccctaat tcattcagta
    14821 agcgcaaaaa gtcgtcatcc gtcaatgaga catcttgggt ctcattataa tggcgtaatc
    14881 tgaaacaacg ctgacaatag acttccccgg aagctaaacc tttttccaaa gccgtttgcg
    14941 gcgtgtatcc taattcaccg ggtttgtttg tttgtataac ggcaccacag ccgatacatt
    15001 gtaattcttc tgtcattcga ttccacctcg ccagcccata tcagggtgtt ttgctaatag
    15061 ataggccata attctttttt cccaaaaacg attaaattgt gtcttccaac tatctgttgc
    15121 gataatgggt tttacaagga tgctacgtat ccctgcacga ttggctccac gtatatcagt
    15181 cataatctga tcgcctacca ttactatctc atcatctgct aaatccaatt gtttctttgc
    15241 tatgttgatc cctcttgcta aaggtttcat tgctcgtgca acatacaaaa gatcaaattt
    15301 ttcaatcgct cgcgcgacac gttttgaatt attgtttgaa acaacaacaa cagggattcc
    15361 tgcatttttc atttctaaaa tccaagtaag caactcttct gttccatctg gattattcca
    15421 tgcaatcaac gtattgtcta aatcggtcaa tactgctttg atgcctagat tctttaattg
    15481 ggcaggtgta atctgataga tggcttctac catccatgtt ggtttgtaat ttgaaaacat
    15541 gctgtctcct catctgcaaa aaatgaagga gcacataaag tactcctcaa ccccgtatta
    15601 tactgcatat cagcaaaaaa aactacctct atactcgttc gtctttcaga aaaagcattt
    15661 ctttacaaaa tgaccaaaaa taaagattga tcacagaaaa atttagaaac aaaaaacaac
    15721 taatattggc ggaacctctt gtatcgtttt tccagtaatt tatgctgatc taatcgaaca
    15781 agttccgtca atctttcaat caacgctttt ttcataatgt gtgcgacttt atatcgtggc
    15841 aactgctcgc cgttgaacgt ttcaggaata actcgatcaa cgattcctaa ctcgcgtagt
    15901 tctggcgcag taattttcat caactcagct gcttctgcgg cgcgactacc gtctttccac
    15961 aaaatcgacg caaatccttc tggagaaagc acagcataaa tcgtttcttc cagcatccag
    16021 acttcatccg ctaaacctag agcaagggca ccgccacttc caccttcacc aatgatgatg
    16081 gcgatgatcg gtacctttaa atcagacatt tcgagcagat ttctggcaat agcctctcct
    16141 tcgccgcgct cttctgctcc tacaccgcaa tatgcacctg ctgtattgat caaggtaatc
    16201 accggtctat tgaacttttc cgcttgtttc atcagtcgta aagcttttcg atagccttca
    16261 ggatggggag aaccaaaatt tctgcggata ttttcctgca gatcccgtcc tttttgaata
    16321 ccgatcactg ttaccggttt gccatctaat cgagcgattc caccaacaat cgcttcgtca
    16381 tctgcaaata aacgatcacc atggcattcc tcgaaagaat cgaagatctc ttcaaaaaaa
    16441 tccaagcttg tccatcgttc tttagctctt gctaactgaa cgacatcatg tgctgtctta
    16501 tccacgatct atccatcctt tctgtgtatg caattcaaga agcgtatgga ttttctgttt
    16561 caattccatg cggggaacaa tctgatcgac aaaaccatga gataataaga attcagcctt
    16621 ttggaaatct tctggaagtt cctgcttgat cgtctgttcg atgacgcgtc ttccagcaaa
    16681 accgatcaag ctttggggtt ctgctagaat aatatcgcct tccattgcaa aactggcagt
    16741 tactcctccg gtcgtcgggt ccgttaaaac agttagataa aaaagacctt cattgctatg
    16801 ccgtttgaca gcagcagaga tttttgccat ctgcattaga gaaaagattc cttcttgcat
    16861 ccgtgcgcca cctgaagctg tgaacaagac gactggaaga ctttcctttg ttgctcgttc
    16921 gaataatctg gtgatctttt ctccgacaat cgttcccata cttcccatga taaaattagg
    16981 atccatcact ccaataatga agggaatccc ctgaatggtt gctttaccag tgaggactgc
    17041 ttcatgaagt cctgttttat cttgcatttt tttaattttt tccgtatatt cgggaaatgc
    17101 tagcggatct ttggttacaa gatccgtatc ccattcttca aaactttttt catcaataat
    17161 caaagctaga cgctgccatg caccgattcg aaaagcgtat ccgcaatgag ggcatacttt
    17221 ttcttcaccg atgtctttcg tataaaggat atgtttgcaa tttggacact ttgcccacat
    17281 gttatcagga acagatggcg agttagcagg tttgctctct ctattaggat tgatccgaat
    17341 atatttcttt ttcttaaaca gagccatcta tgcgcctcct atctccgttt ctggtgtcca
    17401 attcggtaaa aattcttctt gtaaaaatgc tgtgctgtaa tcacctgcta tgactgcagg
    17461 atgactaatc aaatccatct gaaattctgc gttcgtgatg attccttctg tgataaattc
    17521 acttaaggct cgctgcatct tcattaatgc ttcaaaacgc gtatttccat ggacgattat
    17581 ttttgcaatc attgaatcat aatatggcgg gatcgtatat cccgggtaaa ctgcactgtc
    17641 tactcgcagt cccatcccac ctgctggaag cagtaagttt tggatcttcc ctggagaagg
    17701 agcaaaatgg aaagcaggat tttctgcatt aatccggcat tcgattgcgt gtccttggaa
    17761 tactacttct tcttgacaca cattcaaagg ctcaccggct gctatctcca cctgtttttt
    17821 tactaaatcg attcctgtga ccatttctgt tactggatgt tccacttgga ttcgagtatt
    17881 catttccatg aaataaaaat cgccttcttc atccatcaga aactcgatcg ttccggcatt
    17941 ttcataattg actgctttag ctgctcgaac agcagcgtct cctagtgctt ctctttttgt
    18001 ttgtgagatc actacagaag gagattcttc cagtactttt tgattgttgc gttgtaaaga
    18061 acaatctcgc tcgcctaaat gaatgacatg accaaatcga tcacctaaga tctgaacttc
    18121 tatatggcga gcaggataaa tgattttttc caaatacata tcatcatttc caaatgccgc
    18181 tgctgcttct tgctgagctg aagaaaaatg ctgtgggagc tcttcttttg attgaacttt
    18241 ccgaatgcct ttcccgccac cacctgcagc tgctttcagc atcactggat aaccaattct
    18301 gtctgctatt tccaatgctt cttctactgt agataataca ccagtacttc ctggaatgac
    18361 tgggacattt gcttcttgca tcaattgacg cgcattgatc ttatttccca ttgcatcgat
    18421 cgttgcactt ttaggaccaa taaatgtgat attgcactct tcacacatgg aggcaaatcg
    18481 gctattttca gataaaaaac caaatcctgg atgaattgct tcggcttttg tcacgattgc
    18541 agcgctcagt acttcctgaa cattcagata tgaatcaacg gcttttgccg ggccaatgca
    18601 gattgcttca tctgctaact gtgtatgaag tgcttcggca tctgcttctg aatatatcgc
    18661 tacggtttgg atccctagtt cgcggcatgc acgaatgata cgtacagcga tttcaccgcg
    18721 gttagctatc aatactttag aaaacatatt tcacctatcc aatcataaag gttaattccg
    18781 cttcggctac ttttttaccg tctactttag cgattccttt gcctattccg gctgaagctt
    18841 tcactttcaa tatctcaact tcaaggtata aggtatctcc cggtgtaact ttcttacgga
    18901 attttgcttt gtccaatccg ccgaaataag ccgtctttcc cttgaattgt tcaagagaaa
    18961 gtaatgcaac tgctcctgct tgagccattg cttcaacaat caatacccct ggcataacag
    19021 gttcttctgg aaaatgtcct tgaaaaaagg gctcattgat cgttacattt ttcttagcaa
    19081 cgatccgttc tccttcaacc atttcttcca ctcgatcaat caaaagcatt ggataacgat
    19141 gtggaatgat ttcttttatt tcttgtatgt tcattgtttt ccctctttcc tgctttgctt
    19201 atttttgctg aatcctaaag agtggctgtc catattcgac aactgactcg ttttctacaa
    19261 ggatttctgt gatcactccg tcggcctcac tagttatctc attcatcagt ttcatcgctt
    19321 caataatgca gataacttct cctttggcaa cttgatcacc tacttttttg aaagctggtt
    19381 tgtcgggcgc tggttgcaga taaacgattc ccacgattgg cgaaacaacc tcgattccat
    19441 caaatactgc cgattctttt tctttcatta tttctggagc agttgttgct tgttcaggta
    19501 tgacattgac tggctccgaa actggcaacg tttcgtttgt ttgaaccata ttcatgcctc
    19561 gacttgtttt atttttattc atatacaatt caaattgacc ttcacgcaaa tcgaattccg
    19621 ttagcgagga ttgatcaaat tgactgacta attctctgat ttcattgata tccaaaatta
    19681 ttcctcccag cgtttcatgc acagaaccgc attatgtccg ccaaacccta atgaattact
    19741 cattgcataa gtaaaacttg caggtttgct ttcgtttttg acaactgtca agtcaatctc
    19801 aggatcttgt ttttctacat tgatcgtagg tgggataaat tgatgttcta atgccagcaa
    19861 agttgccaag gcttcgatcc cgccggctgc tcccaataaa tgtccagtca tcgatttcgt
    19921 gctgctgacg taagtatctc tgaaattttc acctaatgcg atttggattg cttttgcttc
    19981 tgcctggtca ttggctggtg tacttgttcc gtgagcattg atatatccga cttgttccgg
    20041 ctgtacacca gcttcttcca tcgcaagctt cattgcttta gatgcaccag aaccatctgg
    20101 agtaggagct gtcatatggt aagcgtcaca gtttgcaccg tatccaacga tttctccata
    20161 gattttggca ccgcgagctt gtgcatgttc taaagattca agaactagaa cgcctgctcc
    20221 ttcacccatc acaaaaccat tccgattttc gtcaaacgga atcgaaccac gattaggatc
    20281 agttgattct gagagtgctg ttaaagctgc aaatccagca atccccatct cacaaatcgt
    20341 tgcttctgtt cctccagcaa gaatgacatc agaatagcca tgtttgatgt tacggaatgc
    20401 ttccccaatt gaattcgttc cgctagcaca agctgttacg atagctgtac aaataccttt
    20461 tgcacctaca cgcaaagcaa tatttccagc tgccatgttg gcaatcgcca ttggtacgaa
    20521 cattggagaa actcgttttg cacctttttc atgtagacga ataacttggt tttcgattgt
    20581 tggaagacca ccgatcccag atccgatgat gacgccaaac cggtcttgat tcattttttc
    20641 tttatctaaa ccgctcatct ccattgcttc taaagcagcg tagattccat agatcgaaaa
    20701 catatccatt cgtttgctat cttttttaat aaaatattta tcgtaaggga aatctttgac
    20761 ttcacccgct aaggtaatcc ctgtttctga agcatcaaat ttagtgatcg gtgcgatgcc
    20821 attcgtcccc tcttttaagc tattcaaaaa gctgtctgcg tcatttccaa taggtgaagt
    20881 gactccatag ccagtaatta ccactcgatt catttgtttg ctctcctttt cttatccgtg
    20941 catgaccaat ccgccatcga cattgatgac ttgtccggtg atatatggat tttgtgctaa
    21001 gaaaactgct gtttgtgcaa tatcttctac ttgtccgaaa cgctgcatcg gaatttgctt
    21061 ttccgcttgt tccttgactt tgtctgctaa aacttccgtc atatcagttg tgatgaatcc
    21121 tggcgcaatg gcattacatg taatgcctct agttgcagct tctcgtgcca cagattttgt
    21181 taatcctact acgccagctt tactagctgc ataatttgct tgaccaatat ttccaatcaa
    21241 cccagataca ctagagagat taatgatggc cccttctcgc tgcttcatca tttttttcaa
    21301 tacgtgctgc gtcatattga aggttccaat caaattgata tccagacatt ttttgaaatc
    21361 ttctgcatcc attcgcatca caagtttgtc atttgtgatt ccggcattgt ttactaacac
    21421 atggatcgat ccaagctttt cttctgcttc tttgatcatt tgtcccgctt tctcataatc
    21481 agagatgtca ccggaaactc ctacgcattt tacaccgaat gcttcgattt cttctatctt
    21541 ttcttttgga atctctccgc gaccattcaa aatgatattt gctccagctt ttgcaaaagc
    21601 taaggccatc gctttgccga ttcctcttgt actacctgtg ataaatacat tttttccttt
    21661 tacatccata acgttcctcc ttatagtatt gctattgttt catcaaatgt ctgcttattc
    21721 tcaacacgca gaactggtat tgttttatcg atttttttca taaatccgct taatactttt
    21781 ccggggccga cttcgatgac ttgttcgatg ccgattgttt ttaacttatg gatactctca
    21841 tagaaacgaa caggttgcat gacttgctgt tcaagtagtc ccgcgattgt ttctttcttc
    21901 attatttccg ttgttgtgtt gcttatgata ggaaaagaag gttcagagaa ttgaatctga
    21961 cttaggtcct tcgccagttt ttttgctgcc ggttctaaaa tagctgtatg aaaaggcccg
    22021 ctgacattta gagggatcat tcttttgaac cctttttctt ttaatagtaa aacagcttcg
    22081 tctactgctt tttcttcgcc accaatcacg atctgctgag gtgtattata atttgcagga
    22141 gatacgatgc cgtacttgct agcttcatga cagctttcct cgatcgtttc aatcggcgca
    22201 ttcatcactg caaccatttt cccgcttcca gctggggcag cttctgtcat ataagcgcct
    22261 ctttttgcta ctaacgcaac tgcttcagtg aaggatagcg cattactagc tactaatgcc
    22321 gaatattctc ctaaactcag tcctaaagct gcatctggga tgattccatg ctccttcaat
    22381 aagcgataat acgcgatgct gactgttaga atagctggct gtgtatattg cgtctgatcc
    22441 aaacgttcat tttctgtaaa gcaaagttca gccatatcgt agccgagaat ctcacttgct
    22501 tcatcaaatg tctgctttac aacggcttct tcatataaat cttttcccat accttgatat
    22561 tgtgcacctt ggccactaaa taagaatgct gttttcatgt ttttctccaa tatatccaga
    22621 atatatgttt gattcttcct attcaaaaag gccgagacat tcctatttca ggaaatgtcc
    22681 cagcccccaa cctaatttta atgattatgc agtttgtttt tctacgtaag tcactaaatc
    22741 ttgaaccgtt ttgattccgt cttctgtttc gattttcacg tcaaattcat cttcaatttc
    22801 attgatgatt tggaataaat ccaagctgtc agcttctaag tcttcttgga tgtcagtagt
    22861 caatttgatt tcttcagttt cttttcctaa ttcttctgcg atgatttctt gaattttttc
    22921 gaataccata ataatatatt cctccgatat tgtttgcttt ttatttttat aatttaacag
    22981 tctacaactc catgagaata cttccccatg ttaaacctcc gccatagcct gtaaatatta
    23041 ctttctgctg ttttcctaag gtgatctttc cactagaaac tgcttcatcc aataagatag
    23101 ggatgcttgc agcagatgta ttcccatatt tatccatatt ggtcaggaac ttttcttgcg
    23161 gcatctttac ttttcgggca attttctcaa taattctgag gtttgcctga tgcaaaagca
    23221 gataatcaac tggtttgtct ttcatgactt cttggatatt gtttgcaacg tctcgtacgg
    23281 caaaatcaaa aatatctctg ccagtcattt gcaaataata cgaaccttct gaatcttctg
    23341 aatagaacgg atttttattt tgctggtagc cagcggtcaa tgagctactt ctggtaccgt
    23401 cagctgctaa tttttctttc agcaattttt tctgtggatt cgcttctaag agaatcccac
    23461 ctgctccatc gccaaacaac acagcagtcg tacgatcaga ccaatcgatt agcttagata
    23521 gtgtttcacc accaatcacc agtcccttag ttgagccgca gcggatcatt ttttcagcca
    23581 tgcttaacgc ataaacaaat cctgagcacg cagcactgat atcaaaagcg atagcatgag
    23641 ttgctcccaa tttcccttgt acttgtgctg ctacagatgg tgtcgtgtaa tcgggcgaca
    23701 ttgttgctac taaaataaaa tccaaatctt tagggtttag atttttctgt tttatcaatt
    23761 tttccgccac taaaatgcat aaatcagaag tctcctgatt tgttacacat cttcgttcac
    23821 gaatacccgt ccttgacgaa atccattcat cagaggtatc catcatcttg gaaagatcat
    23881 tattagtaat aatcttttca ggaaggacgc tggctgttgc agtaatcatt ccaaattgtt
    23941 ccattaaata tcctcactta tagtcttgca agaagtcgtg caagtttttc agtgcatcca
    24001 gcaaggcatg ctgttcctcc gtagccattc cgtctaagat gttcttgacc atttcacgat
    24061 gaaaatgctg atggacccga aatagtaact ttcctttttt cgttagtccc aactttacaa
    24121 cgcggcggtc atcttcactg cggatacgtt cgacatagcc tttttttact aacttgttaa
    24181 tagcagtggt caaggtccca acagtaattg atagttcttt ggctacttct gaagttgttt
    24241 ttttcttgta catcccgatt gcctcgatcg tgtgcatctc tgtgattgac agatctttga
    24301 attgagattt tttcagctca gactcttcga ttgtcaaaat atcattgaag acactaacaa
    24361 gataatcatt tactgttttt aaatctggtt ccatgttcca ccgtccacta attagtttga
    24421 ttatcaaatc ttttgatgtt caaactatat cttaactttt ttcaaaatgc aaccttaaaa
    24481 gatcattttt ttactatatg agcttttttt aagatt
//
LOCUS       DARDHA010000022        23884 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000022 DARDHA010000000
VERSION     DARDHA010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.022"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(54..788)
                     /locus_tag="SDA10_000765"
     CDS             complement(54..788)
                     /locus_tag="SDA10_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEG4239695.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="SDA10_000766"
     CDS             1115..1525
                     /locus_tag="SDA10_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEG4239696.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="SDA10_000767"
     CDS             1518..2219
                     /locus_tag="SDA10_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEG4239697.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="SDA10_000768"
     CDS             2219..3805
                     /locus_tag="SDA10_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4239698.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="SDA10_000769"
     CDS             3824..4045
                     /locus_tag="SDA10_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEG4239699.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="SDA10_000770"
     CDS             4042..4803
                     /locus_tag="SDA10_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEG4239700.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="SDA10_000771"
     CDS             5183..5692
                     /locus_tag="SDA10_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEG4239701.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="SDA10_000772"
     CDS             complement(5767..6306)
                     /locus_tag="SDA10_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEG4239702.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="SDA10_000773"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="SDA10_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEG4239703.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="SDA10_000774"
     CDS             7319..8170
                     /locus_tag="SDA10_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEG4239704.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="SDA10_000775"
     CDS             8198..8833
                     /locus_tag="SDA10_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEG4239705.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="SDA10_000776"
     CDS             8853..9626
                     /locus_tag="SDA10_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG4239706.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="SDA10_000777"
     CDS             10034..10831
                     /locus_tag="SDA10_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEG4239707.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="SDA10_000778"
     CDS             10809..11222
                     /locus_tag="SDA10_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG4239708.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="SDA10_000779"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="SDA10_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEG4239709.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="SDA10_000780"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="SDA10_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEG4239710.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /gene="maf"
                     /locus_tag="SDA10_000781"
     CDS             15999..16559
                     /gene="maf"
                     /locus_tag="SDA10_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEG4239711.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="SDA10_000782"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="SDA10_000782"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEG4239712.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="SDA10_000783"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="SDA10_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEG4239713.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="SDA10_000784"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="SDA10_000784"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEG4239714.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="SDA10_000785"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="SDA10_000785"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEG4239715.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="SDA10_000786"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="SDA10_000786"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEG4239716.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="SDA10_000787"
     CDS             21906..22484
                     /locus_tag="SDA10_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4239717.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="SDA10_000788"
     CDS             22536..23018
                     /locus_tag="SDA10_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEG4239718.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="SDA10_000789"
     CDS             23135..23509
                     /locus_tag="SDA10_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239719.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7809 a         4289 c         5123 g         6663 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgag
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcacccggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatagaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaata atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtttt tgagagtata aaatattttg tgtaaatgaa aaaatccata caaaaaagga
    23881 agtc
//
LOCUS       DARDHA010000023        23311 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000023 DARDHA010000000
VERSION     DARDHA010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.023"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(169..1320)
                     /gene="queG"
                     /locus_tag="SDA10_000790"
     CDS             complement(169..1320)
                     /gene="queG"
                     /locus_tag="SDA10_000790"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEG4239720.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(1339..2091)
                     /locus_tag="SDA10_000791"
     CDS             complement(1339..2091)
                     /locus_tag="SDA10_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239721.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(2232..3182)
                     /locus_tag="SDA10_000792"
     CDS             complement(2232..3182)
                     /locus_tag="SDA10_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEG4239722.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(3183..4022)
                     /locus_tag="SDA10_000793"
     CDS             complement(3183..4022)
                     /locus_tag="SDA10_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG4239723.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(4019..4765)
                     /locus_tag="SDA10_000794"
     CDS             complement(4019..4765)
                     /locus_tag="SDA10_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG4239724.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            5003..6373
                     /locus_tag="SDA10_000795"
     CDS             5003..6373
                     /locus_tag="SDA10_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4239725.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(6428..7324)
                     /locus_tag="SDA10_000796"
     CDS             complement(6428..7324)
                     /locus_tag="SDA10_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG4239726.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(7438..7608)
                     /locus_tag="SDA10_000797"
     CDS             complement(7438..7608)
                     /locus_tag="SDA10_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239727.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(7605..8282)
                     /locus_tag="SDA10_000798"
     CDS             complement(7605..8282)
                     /locus_tag="SDA10_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG4239728.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(8373..9278)
                     /locus_tag="SDA10_000799"
     CDS             complement(8373..9278)
                     /locus_tag="SDA10_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEG4239729.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            9453..10907
                     /locus_tag="SDA10_000800"
     CDS             9453..10907
                     /locus_tag="SDA10_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4239730.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            10923..11954
                     /locus_tag="SDA10_000801"
     CDS             10923..11954
                     /locus_tag="SDA10_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG4239731.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            11961..13148
                     /locus_tag="SDA10_000802"
     CDS             11961..13148
                     /locus_tag="SDA10_000802"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEG4239732.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            13304..14686
                     /locus_tag="SDA10_000803"
     CDS             13304..14686
                     /locus_tag="SDA10_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEG4239733.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            14658..15044
                     /locus_tag="SDA10_000804"
     CDS             14658..15044
                     /locus_tag="SDA10_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239734.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(15244..15912)
                     /locus_tag="SDA10_000805"
     CDS             complement(15244..15912)
                     /locus_tag="SDA10_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEG4239735.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(16101..17527)
                     /locus_tag="SDA10_000806"
     CDS             complement(join(16101..16954,16954..17527))
                     /locus_tag="SDA10_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG4239736.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERERIEELEKRVRSLQIENA
                     FLKELRKLRKQEAQQRRMKQSHESSQASEDRFKLVELLETLKFPKAIFMYWQKRLDRK
                     NPDQEIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRK
                     SRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLD
                     PFMDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYR
                     NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYY
                     NNERIKQKLNWQSLVQFRKTTVTVV"
     gene            17770..18225
                     /gene="tnpA"
                     /locus_tag="SDA10_000807"
     CDS             17770..18225
                     /gene="tnpA"
                     /locus_tag="SDA10_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEG4239737.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            18490..18588
                     /locus_tag="SDA10_000808"
                     /pseudo
     CDS             18490..18588
                     /locus_tag="SDA10_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(18811..19041)
                     /locus_tag="SDA10_000809"
     CDS             complement(18811..19041)
                     /locus_tag="SDA10_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HEG4239738.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(19271..20089)
                     /locus_tag="SDA10_000810"
     CDS             complement(19271..20089)
                     /locus_tag="SDA10_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG4239739.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            20250..20939
                     /locus_tag="SDA10_000811"
     CDS             20250..20939
                     /locus_tag="SDA10_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEG4239740.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            20953..22455
                     /locus_tag="SDA10_000812"
     CDS             20953..22455
                     /locus_tag="SDA10_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4239741.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            22468..22944
                     /locus_tag="SDA10_000813"
     CDS             22468..22944
                     /locus_tag="SDA10_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4239742.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(23041..23151)
                     /locus_tag="SDA10_000814"
                     /pseudo
     CDS             complement(23041..23151)
                     /locus_tag="SDA10_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         6973 a         4376 c         4312 g         7650 t
ORIGIN      
        1 aatgaaaaac gcagattctg taacattcac tgcaataatc cataaaaaaa agcaaaaaaa
       61 tttttgattt ttatcaaatt ctcttaaaaa aatgtaaaaa atacaaaaaa accagtaatt
      121 gttacaacaa ttactggaca aaaactttga ataatgttaa ctattttttt attttaactt
      181 tctccgcaat aatttctggg catgttccaa ttcgtttata acgacttctt ctgtttcttt
      241 agctaatatt tcatctaata catcaagcca tttttccggt tcttttgttc ctagacgtcc
      301 aatagcccaa gctgctgttc ctctgatgac agggcggaca tctgttaaac attctgaaat
      361 ctcaggcaat gccgttcgat cacctagatt agctaaagca atcaatgcgt ttctctgcaa
      421 aggtttcttc ccacgccatg aaccagctaa atgcccaaat tgctgtttga agtctttatt
      481 agacatcgaa agcattggct ttaattttgg atatacttcg tctacttttg gttccatctc
      541 ttcatgaaaa tgaaaatctt ttccttgatt gtaagggcag actaactggc agatatcaca
      601 tccataaatg acatttcgca tttttttacg atattcttcc ggcatcatcc cttttgtctg
      661 agtttgataa gacaaacatc gctgggcatt cattcttccg tcaccaagca atgcttttgt
      721 cggacaagca gtcacgcagc gcatacagtc accgcaacca aattctcctg gtttatcagg
      781 ctcaaataca atattagtaa cgatctcacc aagatagaca aatgaaccga actcttcagt
      841 gatcaacaat ccgtttcgtc caataaatcc taaaccagct cgctgtgcta cagcgacatc
      901 gatcagttct ccagtatcta cttgcggagc tagacgccat tcctcttctt tttgccattt
      961 ttctgctctt gtttggatga attcgatcaa tcggttcaaa cggtctctga gtatatcatg
     1021 gtaatcgatc ccccaagaag cacgagcaaa ctgcccgcgt ttttcatccc taggcacttc
     1081 ttcatggatc ctagtaggat acgcaagtgc aatggaaatg atcgatttcg gctgatcaaa
     1141 tgtcatttct ggataaagac gctcatcgat atttggatgt tcaaagccag aagtatggcc
     1201 agctgctttt tgttcttcca acgattcttt taatgaatca aaaggttccg ccgtggtgaa
     1261 cccgattttg tcaattccta atcgtttgct ttcttgtatg atttcttctt ttaatgtcat
     1321 ggtcttcctc gcttgctgtt aacggctgta taacccataa tatagttttt cttttttcag
     1381 ctggacaaac tggatctgct gatcggcaat cttcttttct tccatccatt tttttcttgc
     1441 agcaaatact tcccaataat ttgttgaaat taattttctt ttgttatcta atgcattaaa
     1501 aaaattcaat gaaactggtt ttgtacttaa cagttcatgg agccttacct cttcccatgg
     1561 cgaaaactca gacagatcgc gggaatacaa tacttcttca tagagatttt tttgctgtgt
     1621 gtacgtgaat gtttgcggga ttttcccagg cctagcaatt tctactagac gattataaaa
     1681 catattcatt aatttgctgt tggcaataaa ttgactttta taataggccg actggatctt
     1741 tttgattact ccttctgctt tgatgactga aaataatgca ggccatgctt tgacggttat
     1801 ttcgtcgttt tgtttaacta atacgccagg tgtgaagccg actggagaaa ggaacacaaa
     1861 ataaccgata ccggtatttt catctaaagg ctgaaattca tcaaacaacc atttgcctat
     1921 gtacagctct tcatctggtt ctgttgtctc attttgattt gctatgaata atttagctaa
     1981 ttctgcttca agaaattgaa ggtcttgatc cttgtcggta ttttcctgct ggctgatcaa
     2041 tcgaaagact tcttctttaa tcgtatattt atctttacgt gtaaaattca tagccacacc
     2101 ttccttcctt actctaacta taacggaatt tcttcaaaaa aggtatctgt acgacgaaaa
     2161 acagcataat tttttttcat atctcttata aaaaatgcaa ggtctgctcc tcaagctaga
     2221 ccttgcactt ttcagctttc ttttgtttgt gacaaacctt catggatcgt ttcaatattc
     2281 catttcatca tctgatagta agaatctcct tgttctcctt cttttgctat ggaatcagtg
     2341 aacagtttat catagatttt caaacccgtt tcttttgcta cccgttccat gcttcttgag
     2401 tctacactgg tttccacgaa taaaaccggt acttcttttg ctcttatctg atcaatgatc
     2461 gctttcattt gatcaggcgt accttgactt tctgtattga tctcccatat ataagctgct
     2521 ggcaaatcat atgctcgtga aaaatacttg aatgcccctt cacttgttac taataatttt
     2581 ttgttctctt cgatagaagc aaaactttcc ttcgcttgtg tatctaattc ttttagtttt
     2641 tccacatatg cttctgcatt ttttttatag agtgtcgcat tttctgcatc tttttcagag
     2701 aatatacgtg cgatttcctc cacatatttt attccgttag ataggtctag ccaagcatgg
     2761 ggatctgctt ttgtatgttc ttcaccgcta gttaaataaa gaggttctac atttttgcta
     2821 actgcaaaat aatcttttcc ttcttctttt ttagccgttt ccatcaagtt atcgaaccag
     2881 ctgttacctg tttcaagatt caaaccgttg tataaaataa catctgcatc gcttgccttt
     2941 ttgatgtctt ctggtagtac ttcatattca tgcggatctg ttccgactgg tacgatactg
     3001 tggatatcta tttgatctgt tccgacctct tttgccatgt ccgccaagat cgaattggta
     3061 gccacgactg acaacttttc tttacttcct tcatccgctt ggtcagaagc ctgctttccg
     3121 caaccggcta ataccaagaa actaactgcc aagattaaaa agagtgattt ttttgttttc
     3181 atctatctgc ttcctttctg aaataaaatt ccttttgttg gagaaaataa aaaagcaagg
     3241 ataaaaaatg cggtacatgc taacacgatc gtagctcctg atggccaatt gtaactataa
     3301 ctgaaaaaca caccgacaac tgcacttagc ataccgattc ctgcagctgt aatgatcatt
     3361 ttcagtaaat ggttcgttag caagtaagcc gtggcggctg gtgtgatcaa catggcaatc
     3421 accaagattg ttcctactgt ctgtaaactg actacagcca ctaatgtcaa aaagaacatc
     3481 aaaagataat ggatcagcga tgtgttcagc ccataagctt ttgccattgt cggatcgaat
     3541 gatgtgatct tcaattcttt ataaaagaaa aagacaaata tcaagacgat tccactaacg
     3601 agtgctgtca gcaacaaatc gctttcccta acagctaaga cattcccaaa taaaatatga
     3661 tacaaatctg tcgcactctg ggcatatgaa atcaagataa tccctaatgc aaaaaaggag
     3721 ctaaagacaa ttccaatcgc tgtatcattt ttcaatcggc tccgctgagt cacgaaacca
     3781 atcactgctg ctgttagcat cccaaaagca gtggctccaa agatgtaact aaaaccaaac
     3841 atataagaaa tggctacacc tggaagaact gcgtgagaga tagcgtctcc catcaaagac
     3901 atccctcgta aaataataaa agaaccgatg actccagaag caagtcctac aagcatggaa
     3961 gtgaccaaag cgtattgcag gaattgataa ttcattaatc catcaataaa ggattggatc
     4021 attttttctc tcctcctttt actaaatgtc tgatgatctc accataggcc aattgaaggt
     4081 tcttcgatgt aaagacttct tgtactggac cggaagcgat cagctgttta tttaaaagaa
     4141 taacctcatc gaaatattct tctactttat gaagatcatg atggacgatc aaaatcgttt
     4201 ttcctgaatt cttcaattcc tgcaagatgt caaagatctt tcgttcactt aacgcatcaa
     4261 tccctacgaa tggttcatct aaaaagatcc attctgctcc ttgggctaga gctcttgcaa
     4321 taaaaactct ctgaagctgt ccacccgata gttcgctgat ctgtcttttg gcatattctt
     4381 ccatccctac ttttttcaaa gcttgtcttg cacgttcttt ttcttttttc ccaggtcttt
     4441 gtccaattcg taaagatgga tatgttccta aaagtacaac gcctaataca tcaattggaa
     4501 aggaaagatc caattcactt cgctgttcca cgtaggctat ttttttacgg actgagttta
     4561 ccggctgtcc agaaaaggtg actttaccag tagtagctgg tatcaactct agcaaagatt
     4621 tcataaatgt tgattttcct gcaccgtttg gtccgataat accggtaatt ttttgtggat
     4681 ttatcgtcaa gttgatagtt ttcaaaacag ttttcccttg ataagcggct gaaaggtctt
     4741 gaattacaat ggccgtacgt tccaaatctt acactccttt ttaggtttac ctaacttttt
     4801 atttaaatat agtttaacta aaatggaaag cattttcaag tagaatgatg aaaaagtttt
     4861 tttcgaattt tcctcgcatt ttatgaaaat tttctgtaga atcacatcaa gagttcaata
     4921 aggctaacct aataatgaaa aacatgtgtt gaaacgtttg attatttttt cttgttgact
     4981 caacaacgaa aggagttttt gtatggaaga agaaaagtta actcgaagtc ttagtgcccg
     5041 tcatatccaa atgatcgcac taggtggaac gatcggtgtc ggtctgttta tgggtgcctc
     5101 ctcaaccata cgttggacag ggccctcagt gatgggagcc tatgcgattg caggattctt
     5161 tttgtatttg attatgcgtg ctttaggcga aatgctttac gttgatcctt cgactggttc
     5221 ttttgcaaat tacgcgagcg agtacatcca tcctgtggct ggctacttga ccgcttggag
     5281 taatattttt caatatattg tcgtaggaat aagtgaagtg attgctgtag gatcttatat
     5341 gaattattgg tggcctgatt tgccggcaat tattcccgga atcattgtcg tcttgtttct
     5401 tatgctggca aatcttattt ctgtcaaggc gtttggcgaa ttagaatttt ggttttcgat
     5461 catcaaagtc ataacgatca ttttgatgat cattgcaggt cttggggtaa tcttatttgg
     5521 atttggtaat catggtcagg cagtagggat ttcaaatctt tggaaaaatg gtggattctt
     5581 cactggaggc gtcaaaggat ttttctttgc attatcgatc gttgttgctt cctatcaagg
     5641 aatcgaactg atcggaatga ctgctggtga agcagagaat cctaaaaaga cgatcattga
     5701 agctgtccag tccactatcg gtcgaatctt gattttttat attggtgcta tttttatcat
     5761 tgtgagtatc tatccgtgga atcaattgag tgagattggt tcaccttttg tacaaacttt
     5821 ttctaaagtc ggcattacat ttgctgcagg actgattaat tttgtcgtga ttacagcggc
     5881 attatcaggt tgcaattcag ggattttcag cgcaagtcgt atggtctata cacttgccat
     5941 aaatggaaag atgtctaaaa aatttctgaa actatctcgt cacggtgcgc ctttttatcc
     6001 tgtcgtagca atctcaggtg gaatcttaat tggtctgatc ttaaattatc ttctaccaat
     6061 gttcatcaac cagtcagaaa atattttcgt tttcgtatac agctcaagta ttttgcctgg
     6121 tatggttcct tgggtagtga ttttgatcag cgagattcgc ttcaaaaaga agcacgcaga
     6181 taaaatgcat gatcatcctt ttaaaatgcc actatctcct tatacgaatt atgtgacact
     6241 tgctttttta gctatcgtat tgattttcat gtttatcaat cctgaaacga gaatctcatt
     6301 gattattgga ctcgttttct tgctttacat gacgatccat tactttgtac gcgaaaaaag
     6361 aaccggaaaa taaaaaaaga acgaagcttc accttagtgg aagcttcgtt cttttttatt
     6421 gcttttatca attcaatttt agcgtctcac cgggagtcag gaaataattc tccatgctca
     6481 tcccattcag cgacaagatc gtttctactg gtacgccagt tcgttcggct atttgatttg
     6541 gaccttctcc ttccaaaact acggttgtcg cagcctgtga agtcgcgctg ttggtattag
     6601 tttcagagga atctgttgtg tcttgtgttg ttcgagcttg catttcagaa gcttctaccg
     6661 actctgctgt ctcagaatca gaagatgttg tgtcttgtgt gcttgttgac tctgcttgaa
     6721 gagaggcgct agtctgcaaa taaatatcgg aaatcttagc gacatgattc gtataattag
     6781 ccgataattg attgatcaaa gtatctaagt ctacaccgtc tttattttct gctgcctgat
     6841 acgctgcacg taattttctg atcccttcgt cggagattgc ttgaagctca cgtgcttttt
     6901 ggttagcaac tgctgataaa ccagctattc cctcttgatt tccctgaatt tctgtctggt
     6961 attcttcaat caatgcaggc gtcttttctt cgatcttttt cgaataatca tttacgattg
     7021 cttgaagttg ctctggcaaa ccatttgctg ttttttcttc agaagtttgt gtctcttttg
     7081 ttttagttga tgactcactt gtttgtttcg tctctgtttt ctttgtcgtt gtttccgtta
     7141 tggtcgtact tttcgcttct gacgaatttt ttccatgaaa atatagaccc gcaactcctc
     7201 ctgctatgat caaaaacacg gcaactgctg ccccaatcat gatcatccgc ttacgagctg
     7261 cctgtttttg ctgtcttctt cgggtcatcc gaacattttc tccccactca ttttcatcat
     7321 acattttttt caccaccaat ttttatgtat aatagcgtta tcaaacgctt catattctta
     7381 ttgtagcaaa taattgtcgc tgtgcacatt tttatctttg ttatttataa gaaaatttca
     7441 ccagttgaga tgattccaag attgtccgtc gatttctcct tgcagctgca ttgttcgttc
     7501 gtctatctca gccatcagtt tctttatttc atataagagc gcacggtctt gatattctga
     7561 tttttcgctt aattcatcta attgttggat cagccaatca tttgtcataa tgaagttcct
     7621 ttcatttctt gcagtgtaaa ggtcaagtct tcgatctgct cttttgcctc agtcagtgct
     7681 tctacgatat atgcttgttt ttcagattct tcttcctgca ttttctgatt ttcctgtact
     7741 ctttgaataa accagtcaat ccgctgagtc agcgatggtt tgccttccaa tgagagatac
     7801 tgctgataag caattagctt ctcccaacgt ttttcctggg ataaatacat aaattgtgct
     7861 aaaacgaatg gcggaagata ggtcatatgg aatttatttg cgattcgctg atacggcctc
     7921 aaaaattcgg aaatcgtata ttcttctttt cctccagctt ggtattcttt ttccgccaca
     7981 cctgttgtca atacgattcc tagttctttc ccttttaata catgtgctgc tcgttccaag
     8041 acattatctt gccaaatttt taaatgagca ggagaactat accagtaaaa cggaaactgg
     8101 aaaatgatcc gatcatgctg ggccaagagc tgtagttcag tctctatatc tatttttccg
     8161 tctggataac ttgattccaa atgatgccaa gtagctggta aaccagcaga agcttctttg
     8221 aaaaattgct gatttaaaga ttcttcgatc gttggatggc taatgatgat aagtgtacgc
     8281 atcccttttc ctccttttta gtcttcgctt tacagtgtag cataaccgcc acaagtttct
     8341 tttgccgatg cttgtcattt taaccagact aatcaggttg tccaagaaac tgcggtttgt
     8401 ttttccaact gttttttcaa ggtatccaaa gcaatattct taaaattttc gtttttcata
     8461 cgtttacgat agttcgtagc cgtttctccg gtactctttt tgaacattcg gctaaatacg
     8521 actagactat tgaaccctac ttgataggca atactttcca cggattcatc cgtcagagaa
     8581 agaagaaacg cccctcgatt gatccgtacc ccgatcaaat attccttgat actggtaccc
     8641 atatgatttt tgaatactcg cgacaaatgg ctccgattga ccgctaaatg ctgggcgatt
     8701 tcctcaacag acaatttttc agcataatgt gcctcgatgt atttgactgt ttcaacagct
     8761 aaagaggaat gaaccttttg tacaccaccg ctgacctttc cgccatcttt taaaaggact
     8821 gataaaaaac ggtaggttaa tgctgttaac tgtaattcat tttccaaggt ttcttccgta
     8881 tctttcatgc attctaaaat aatgtcgata tatttttgta tgtcagaaga gaccatcgtg
     8941 tattggtctt ctctgaaaag cgactgccga atgatttctt ctgcagcact cccaccaaat
     9001 gaaagccagc aatacatcca cggatcttct ccatctgata agtaaaacgt cgagtctcct
     9061 ggacgaatca agaacagatc accttttttc aattggaatt gttgattttt cacatgataa
     9121 gatcctttgc cttccatcac gacatgcagg atgtaatcat tgcgaatagc aggaccaaaa
     9181 gaatgaaacg gcatcgtttt agagaaacca caaaaatcaa aatacaattc tttttctact
     9241 tgttgatgtt ccttttttaa aaaaatcgat tgctccacgt ctatccctcc ttactccttt
     9301 agtataatgc aacaaaaagt taaataaaag tcacagaaat atattaaaaa ggaagcgatt
     9361 tcttgttaag tttaagtcat taaaagatct ttgttattcg ttgtaaccgg tttacttagt
     9421 attatatatt gaacaggaga ggaatacaga ttatgaaaag caaaatgatc caacgtgtgt
     9481 cctacgcttt aggcgctttt ggacacgaca cattctatgt tacattaagc acttatttta
     9541 tggtattcgt gacaagccag atgttcgcag gaacagataa ggcaacgaat gcaaaaatga
     9601 ttgctacagt tacaagctta gttgtaggta tccgcttagt tgaaattatt tttgatcctt
     9661 tgattggtgg catcatcgat aatactcgta caaaatacgg taaattcaaa ccatggttag
     9721 ttatcggcgg cgttgttagt tccgtactct tggtagcaat ctttaccaat ttctttggct
     9781 tagctactca taatacgact ttatttatca tattatttgc aatcgttttc atcttattgg
     9841 atagttttta ttcatttaag gatatcgctt tttggtcaat gattccagct ttgagtacgg
     9901 aaacaaagga acgtgaaaaa tttgctacgt ttgctcgttt cggctcttct ctaggtgcaa
     9961 atggtactac tctagtcgtt gtccctatcg ttactttctt tacctatctt gttactcata
    10021 aacatattga aggcgcttca ggctggttct ggttcggtgt gattgctgct gttatttcag
    10081 gcggtactgc gattatcact gccttgggca caaaagaagt cgatactgcg atccgtcagc
    10141 aaaatgaaaa agctgggatc aaagatgtgt tcaaagcagt tgctcaaaat gatcagttga
    10201 tgtggcttgc ccttgcttat atcgcttatg cgattgccaa tgtagcaaca actgccgtat
    10261 tattctactt cttcaaatat gtacttggcc aaccaactga attttggatc gttggtctag
    10321 ttgctgcctt acttggttta gtagctgttc cactattccc aatgcttaca cgcgtcatct
    10381 ctcgtcgcta cgtttacctt ggagggatcg caatgatgat ttcttcttac tttttcttta
    10441 cgatcgcagg ttcaagtttg ccaatcgtta tcatcgggct agtattgttc tacttccctc
    10501 aacaattgat tttcttatcc gctttgatga cgatcacaga ctctgttgaa tatggtcaat
    10561 ggaaaaatgg tatccgtaac gaagctgtta ctctatcaat tcgcccttta ctagataaaa
    10621 tcgctggtgc tttgtctaac gggatcgtag ggtttgtcgc tatcgcttct gggatgactg
    10681 gtaatgctac tgctgctgat atcactgctc atggcgtaac gactttcaaa gcttatgcat
    10741 tctatgctcc tgctgctttg atgattttct ccgctattat ttttgcttgg aagattacat
    10801 tgacagagaa aaaacatgca caaatcgttg aggaattgga aaacaaattg gctccagctg
    10861 atacaatgga agatgaactt cacgatgcag tagttccttc ccattaagaa aggatcttga
    10921 aaatgaagat tacagaaaaa gattttggcc aaggttatca cttgataacc ttggagaaca
    10981 aaaacaagct agcactttct atctctgatt taggtgctag aatcgtcagt ttaaaatcaa
    11041 atgatcgaga attagttttg ggatttgaca ccgctgaaga atatatcgaa aaagatccgt
    11101 atatcggtgc ttctatcggt cgcaccgcag ggagaatcga aaatggccgc ttttctctca
    11161 atggaaaaac ttaccaatta gccacagatc caaaaactgg acataatctg catggtggtg
    11221 ctcctggatt tgaattaaaa aaatggtctt atgtcatttt gaatggcgaa aatgaagctt
    11281 ctgtcatttt tactactaca agtcccgacg gagaacatgg ttttccaggt acaatggatg
    11341 tggaaatccg ctatactttg acaaaagata atatctggcg agtgacctct cgcggcacca
    11401 gtgaccaaga tactttattc aatccgacaa atcatgttta cttcaacttg acgggagatg
    11461 cttctcagtc aatcgatcaa catgagcttt ggctcaatag cgaagcttat gctcctttgc
    11521 gaacagatag tattcctata ggggtaaaag aaaatgctgc aggaagtgct ttcgattttc
    11581 aaatacctaa aaaattagcg tccgtttttg ctagtgattt ggatcaaaaa aatcttgttg
    11641 acggaatcga tcatccattt ttcttaaaag aaaccggctt gggtaaagaa gccgctcgct
    11701 tgactagccc cgataaaaga atccaagtag gtattgctac agatgcttct agtgtggtta
    11761 ttttcactgc aaatttcggc acagaaacac cagaaatgcg caaccgaaaa ctggcacatc
    11821 acggtggcat cacatttgag actcaaacag ctccaggtgc agagcgcttc tcagcatttg
    11881 gttcgattca tttaaaagct ggatcagtat ttgaaacagt gacagaattc aaaatcaaaa
    11941 caagaaagga ataactcatc atggaaatca aaagaagctt aaacacaaaa tttgccgaaa
    12001 cattcggacc taaaaaaact acacaatact tctcaccagg acgcatcaat ttgattggtg
    12061 aacacacaga ttacaatgga ggccatgtct tccccgcttc catcacttat ggaacgcaag
    12121 gtgtagcagc acctcgagac gataataaaa tattagttta ttccacaaac tttgacgata
    12181 tcggtgtcat tagttttact ttagatgagc tagaatatga taaaaaagca aactgggcaa
    12241 attacgtaaa aggaatggtc cttaaattaa aagaagctgg acactcaatc gatcatgggt
    12301 ttgagttgtt agtggaagga acaatcccta atggtgcagg actttccagt agcgcttcat
    12361 tagaattatt agtaggtgtt gtattagaag atctgtttga tttaaaaaca gatcgcttgg
    12421 aactagtcca aacaggtaaa aaagtagaaa atgaattcat cggtgtcaat tcaggtatca
    12481 tggatcagtt tgccatcggc ttcggtgagc tagatcatgc aatcttatta gatacaaata
    12541 cgctgaaata cgaaatggtt ccagtgaaac tagatggtta tgcaatcgtc atcatgaata
    12601 cgaataagcg gcgggaattg gcagattcaa aatacaatga acgccgtagc gaatgcgaag
    12661 aagccttggc acgtttgcaa aaaactttgc ctatctcttc tttaggagac ttagatgaag
    12721 aagaatttga atcaactatt gaccaaatcg gcgatgatac attgatacgc agagctcgcc
    12781 atgccgtttc tgaaaatcag cggactttga aggcaaaagc tgaattagaa gctgggaact
    12841 tagaagcatt tggtcaactg ttaaatgatt ctcaccactc tcttcgctat gattatgaag
    12901 taactgggat cgaattagat acactagtag atgctgctca aaaacaagaa ggtgtcttag
    12961 gtgcacgaat gacaggagct ggatttggtg gctgtgcgat tgctcttgtc aaagaagaaa
    13021 atatacctgc cttcaaaaac aatgtctatg atgaatacat gaaagttgtc ggctatgctc
    13081 cggaatttta tgttgcacac attggaaatg ggacaacaaa gatcgatcca gaagctgttc
    13141 atgtttgata aaaaaatcaa cgatctcagc taaaaacaaa tagataaaaa taacctctca
    13201 gcaatttcca aatgtgagcc ttttgctcgt atttgaaaat tgctgttttt tattgacacg
    13261 gaaagagaac gtgtgttcta attgtgttga agaggtgatt gttatgtggg cgaatcaaaa
    13321 tccggtattt gtttatgaaa aagaaccttc aagagatatt ttatgtattg actgcaaatc
    13381 tttttacgcc tccgtagagt gcgttgaaaa ggatcttgat ccccttacaa ctaaattagt
    13441 agtcatgtcc tatccgtccg attcgacaga aacacggggt agcggattaa tcttggcttc
    13501 cagtcctgca gcaaaaaaag cttacggcat caccaatatc agccgtgcca gagatcttcc
    13561 cttcccctat ccaagtgatt tatatattgt tgccccaagg atggcctatt atatggaaaa
    13621 gaacagagaa atcaattcta tctataaaaa atatgcagac gaagcgaacc atcatgtcta
    13681 ttccgtcgat gaaagcttcc ttgatgtaac aaatagcatg gcactctttg gtaaaacaaa
    13741 tgcgtacgac atggctaaac tgatccataa agatgtgttt gagcagaccg gaatctacac
    13801 gactatagga attggcgata acccgctact tgctaaacta gccttagaca atggctcaaa
    13861 agacggaaag caaatgtttg ctgaatggcg ttatgaagat gtaccagaaa agttatggaa
    13921 aatctcagat atcactgact tttggggaat cggcaaacga acagcaaaac gtctgaaaca
    13981 aatgggtata cattctgttt atgatttagc tcacgcggat tattatcact tgaaaagcaa
    14041 gctaggggtc atcggtactc agctttatgc ccatgcttgg ggcatcgatc gaagtttttt
    14101 gggacaagaa tatacaccaa aatcaaaaag tatcggaaat agtcaaatcc tagatagaga
    14161 ttatacaaga cgagaagaaa tcgagatcgt tatcaaagaa atggccgacc aagtcggcac
    14221 tcgcttgaga cgatctgcag caaaaacaca agtagtcagc ttatgggtag gatactcttt
    14281 aggctatctt gatccaaatg gcaagaccgg gtttcaccag caaatgaaaa tcgaagcgac
    14341 aaatgctagc agagatctag cagatgcttt attgaccatt ttccaccagc attaccacta
    14401 ccaggatatt cggcgtgtag gcgtgaactg ttccgacctc gtatacgcga caggtttaca
    14461 gcttaacctt tttgaagatc ctgaaaagca gataaatgaa gcgaaagttg attttgttgt
    14521 cgatacgatt agaaaaaaat acggctttca atcaatcatc catgcgcata gtctcttgca
    14581 aggaggacgc gccatcgcac gtagttctct cgttggcggg catgcaggtg gtatgagtgg
    14641 attagaaggt ggaaaagatg agacggacag caaaaaaatt cattgactat aacgagtatc
    14701 gagatcggcc atttggatta aaatggggga ctgcttttgc gatggaagaa ctagtcaacg
    14761 gtatcaaaaa aaatgaagaa gaagcacgca aacagcctct tcctaaacaa caaatgacat
    14821 tgcaagaaat cgatagtata ctgacagaag cctttttatc tcgtaaagca gtaatcattc
    14881 agctcaattt aaaagatgaa tttggaagac tcttggatga ggtcgaaggg cacttcattg
    14941 gtgaagccta tgaagattac tttatgattg accatcagcc gatattgtgg gaagacgtac
    15001 ggtatattga gttaaaggaa acaaaaaaat ggtttgtgac atgagaggtt gtgacagaag
    15061 tgatcaactt caaaaaataa gaaaggattt ttcgaaaatt gcttcttaaa tttttgtgga
    15121 attttggctt atttttcgaa gaagcaactt ctggaacaac gtatattaga tatcagaggt
    15181 tgtgaaaaag tcgcttagtt ttgagtaaca agccaaggcg ctgatttcac aacctctgtc
    15241 tatctatcca tctattcggt aagattcatg catgacgtct tctttattta gtatgtagag
    15301 ccctttttct tctgaccatt caacataaat cagctgtttt acatctgcat agccttcttt
    15361 cttcaagttc tccataagcc atgcttcagt caattgattg gactgaagca ctttatgctg
    15421 aatttgccct tcatctacaa gcaggatcga taaagctttg tcttcatctg attttttcaa
    15481 tacactcatt tgaccagcat tctctaggat aacatgtttg gcattttcaa gagaaaagca
    15541 ttcttgctgt cttaagattg ctcgcaactg ctccatctcg atatggttat ttgtcaactc
    15601 tgtcatgttg atgacgccgt ctttgattag aacagatggc tcgcctttga cccaacgatt
    15661 gaccttttca tttttttgga cgatacgttc gataccataa atcattactg cccaaagcgc
    15721 aatggcaaat aggacatgcc ctacatggac attgtcatca taggttgatt cttctaagat
    15781 cccacctaaa acaagcgtat agactaaatc aaacggtgtg atttctgaca tggatttctt
    15841 cccaagcaaa cgtaccacta ataataaacc gatcaacccg aacaccagtt tcattgtaat
    15901 tgaaaaaatc attttttctc cccctaacta tctgttaatt aaattatata tgctttctca
    15961 cgaacacgca tggaatatac aaggaagaaa tgtatccttt tactaaaaaa aggactgtga
    16021 cactgtagtg acccccaaaa gttagacttt tttggagtgg agaaatccac tccttttttt
    16081 tatgctctaa aatgatattt ttacacaacc gttacggttg ttttacgaaa ttggacgaga
    16141 ctttgccagt ttaatttctg ttttatccgt tcattattgt agtaatagat atagctatcg
    16201 atggctgatt ttaattcctc gaaacagtta taaactttcc cgtgaaaaat ctcttgtttt
    16261 aataagccaa aaaaattctc cataagtgaa ttatctaagc agttcccttt ccgagacatg
    16321 ctttgaaaga ttttgtactc cttcaatttg tttctataag ctttcatttg atacgcccaa
    16381 ccttgatctg agtgaaacgt acgacgatag ggacaatcgt tagtggtctg gatagcttcc
    16441 tccaaacctt ccattaccgc taatgcattg ggcttttcag agatacgata ggataaaatc
    16501 tcactattga acatatccat aaaaggatct agatacaatt tcttttgacg gataatgcct
    16561 ttggcatcca cttcatggta tttaaattca gttgtatccg tcgtgatttt ctgatggcag
    16621 atactggtgt agaaacgacg atggatccga tttttggcta ctgctccgac ttgtcctcga
    16681 taagaattgt agcgccgtga tttacgtgtg tatgtcgtta cttttattcc tagcttcttg
    16741 atcaggcgct gaactttctt tttattgata gagaaaccgc ggtttcttag ttcattggtc
    16801 atccgtaaac agccataatc cttgtgcttc tcccgaactt taagcatttc cgcttcaatc
    16861 tcttgatctg gatttttacg gtccaatcgt ttttgccagt acataaagat tgccttaggg
    16921 aactttaaag tttctagaag ctcaactaat ttaaacggtc ctcggaggct tgcgatgatt
    16981 cgtgcgattg cttcattcgt cgttgttggg cttcctgttt tcgcagcttc ctcaattctt
    17041 ttaaaaaggc attttcaatt tgaagggaac ggactcgttt ctccaactcc tcgatgcgtt
    17101 cacgttcttt agacgttgct ttagttgtag attttttctt atttttgggt tcatttttct
    17161 tagacaagat aggtggacat cctttcgttt ttgagagtcc atcgatccca ccctcacgga
    17221 attgacgcat ccaactagcg atcaaattag ggttgttcat gttaagtgtg ttggcaactt
    17281 cacgatagga cagctcactt gttagatata actctatcgc atccagctta aattgaacag
    17341 aatatttttt cttttgtcgc tttcttacta gaccctcttc accaaattct tgataggcat
    17401 tgatccagcg cttcacttgt gatggagcct taatggaata tttctttgcc agataatcac
    17461 ttccgccttt accatccaag taatcatgaa cgatctttaa tttaagttca aaagtatact
    17521 tagccataaa aaataccgac ctccaaaagt tagatttttt ggtctaactt ttgggggtcg
    17581 gtacaaaact taaaagtttt agaccctttt attttcttaa tgacttcagt cagttttgtg
    17641 cgtaaagcgt gcgaaacaag aaaaccgcgc gtttctacgc gtggaagaag tatctttagc
    17701 gataaaaact cctttcgata tactagtgag tggctaccaa ccgctcatca gatcgaaagg
    17761 agttttttca tgtcaaatga cgataaaagt ttagcatata ctcgttggaa ttgtaagtat
    17821 catattattt ttacaccaaa atatcaacga aaagtaatct atgggcaatt acgaaaagat
    17881 cttggaaaaa tacttaggaa gttgtgcgaa atgaaagatg tggaaatcat agaagcacat
    17941 gcaatgctag atcatattca catgcttgtt cgagtaccac caaaaatatg tatatccagt
    18001 tttatggggt atctgaaagg tcgtagtgcg gtactaatcc acgagcagca cgcaaatctc
    18061 aaatataaat acggaaacaa atccttttgg tcgaagagat actatgtgag tacagtcgga
    18121 ttgaatcaaa agacaaatga aaaatacatc cgtgaacaag aagctgatga tcgcattcga
    18181 tatattataa gtaaaagaga gtacgtagaa ccatttaaaa aataaagaga agtaagtgag
    18241 tgaagatgag cagttggcgg tcttttaaaa atcccctttt aaggggcagc gagtgtcgac
    18301 cccttctagg gggattaaaa atacacccgt cctcacgggt gtatgggtta ctgatagact
    18361 tgattttaac tggtgtcaga aacttatgtc cagaaagggt gatgaaattt tggcaattta
    18421 cggctattca agaattagca caagcaacca agattacaaa acacaaattc aaacattaga
    18481 aaatgctggc gcagaaataa tcttcctaga aaatcactgg attatcgaac acctttggaa
    18541 gtatttttga gttacgtaag tattgatgat ctgtctaact taatttgaca attaagagta
    18601 tatttacttc agatggtaag gcaacaaaat aaaatacaat aggcagatga actaataaat
    18661 aaaatctgta ttaaatacag ataaattaat ataaaaatca aaaggatcat aaacttttaa
    18721 caagtgatga aatgggtgga aattgattct tatagacagt ttgtctcata acattgaagt
    18781 attttgcaat tttagcattg aaacactttt ttaatcttca acgttgttct tataaacaag
    18841 atgattggga atcttttgaa ttaacactgt atagcttaga acacccttta tagaaaacat
    18901 tcgcttagca atcttaataa ttccttgtgc ccgatgtcgt ttagattcag taggttcctg
    18961 ctcatcgatc atggaaaaaa tttgaaggat cagatcctta ataaatttat ttaagagagg
    19021 atgatccatt gcttcatcca tactcgataa actagtgata atgagctaca cgtttttctt
    19081 tcaagttgat caatagcttc cacgataaaa atatctttag ataaaatcaa gaaccgtagg
    19141 aactacaggg atatttggtc aataagagcc accgctatta gtaaaaaata agctatcaag
    19201 tatgctctca tcccaagaat tgcctacttc aatcgttaaa aaccgttagg tttagctaag
    19261 tgggggtagt tcactcaata cttttatcaa taatagagcg caaaactttt tctcttaatt
    19321 caactgaatc aatattattg ttttgttcat aaccatgaac taatagatca caaatataaa
    19381 gttgtgatat ttttccagct aaagatccac cattaagaaa ttcttcaatg gctgtttgta
    19441 aaactaagtc tgcagacttt cctataggtg aatgaatata gttagttata gcaataattt
    19501 ttgcgccatt ttttttagct attttcaatg aatcataagt atctttcgtt ttcccagata
    19561 aagaaaaaat gattactaaa tcattaactg ttaataaaga agcgacttgg gcttgaaaat
    19621 gagggtctag tacagctttt gcttgaactc ctacgcgaag aaacatattt tctaaatcaa
    19681 gactagtatt tccactagat cccactccaa atatatatat gttttttgat tgagtgatca
    19741 ttttaatggc ttcattcaat ttttcttcgt ctaaaagcct aatagtggaa tagagagctt
    19801 ctgttaaact attagcaatt tcatcatagt actttacact agtcggatgt tctttttttt
    19861 gactaaagtc ttcctttgca atttcaattt ttaaatcaga aaaaccagaa aaaccaattt
    19921 tttgacaaaa gcgaataata gtagcatcgc caacttttgt gtttttctta atatcactca
    19981 tcgtactata aataatagtt ttcccagaat ttaaaataaa atcagcgact tttttttcag
    20041 acttcgttaa aaatggataa ttttctttaa ttaaatagat ataactcata caaactcctc
    20101 tacaatttca acttttctcc attatactcc aaaataataa aaaaaactcc taaatactcc
    20161 atttttatct ttacttttat gaaagcggat gctatattgt ttttggagtt gttatccaaa
    20221 aataataaaa aattggagtg tttaggataa tggataaaaa gaggttttta gatcttattt
    20281 caaaaggttt gattgtttcc tgtcaagcat tacctggtga accattttac aaagaggaag
    20341 aaagtttaat gccttatttt gctttggctg ctcaaagggc tggagcagtt ggtatacgtg
    20401 caaattctgt tcgagatatt attgaaatta gaaagtgtgt atcattacca atgattggaa
    20461 ttattaaaag agattatcca ccagaatctc cattcattac tgccactatg agagaagtag
    20521 atgaattagt agaaaccggt gttgaggtaa tcgctcttga ttgcactctt agaaatcgac
    20581 atgatggact atctataaat tcttttatta ctaaaataaa agaaaaatat cctcatcagt
    20641 tatttatggc agatatttct acatttgaag aaggcaaaaa tgcatataaa gcaggaatag
    20701 attttgtagg aactacttta agtggatata ctgaggaaag taaaaaacaa gatgggccag
    20761 atatagattt gattaaaaaa ttggtagaag aagggatttg tgtaattgct gagggaagga
    20821 ttcatactcc agaacaagca aaagagataa aaaaaatagg agttgcaggt attgttgtgg
    20881 gtggtgctat tactcgacca caggaaattg catcaagatt tgttaaagca ttaagttagg
    20941 gggagaaata aaatgtttaa aaaattttca caattaggac gcgctttcat gttaccaatt
    21001 gctatattac cagtagccgg attactattg ggactgggag gagcgttaac aaatgagagt
    21061 gctataaatg cttatccatt tttagatcaa ccgtggttgc atacaatttt aagtattatg
    21121 agttatgctg gaaatgcagt ttttgcaaac ctagcactaa tttttgcaat tggtattgct
    21181 gtaggacttg caaatggaga taaaggaaca gctggtttag ctggtggagt agcttatcta
    21241 gtatatactg caactattag tgggtttctt gaactttttt cagcaaaaga tgcaacacta
    21301 gatacaggtg tagtgggatc aattgttgtt ggtggtactg ttgcattttt acacaatcgt
    21361 tatagaaaaa ttgaattacc acaattctta ggtttttttg gaggatcaag atttattccg
    21421 attgtttcta gtttagctgc aatcattatt ggttctttct tttatttaat ttggccacca
    21481 attcaaaatg gtttagtagt tgctggtgaa caaattgccc aaatgggtag tttaggaact
    21541 tttttatatg gttttttatt gcgtttaaca ggtgctgttg gactacacca caccatttac
    21601 cctttgttct ggtatacatc tttaggtggt accgaaactg tagctggatc aacaattgct
    21661 ggagcccaaa atattttctt tgcccaacta gctgatccaa accacactgg tctgtttact
    21721 tatggtacta gattttttgc aggaagattt gcaacaatga tgtttggatt accagcagca
    21781 tgttatgcaa tgtatcgtgc aattcctaag aaaaatcgta aaaagaatgg tgggctttat
    21841 tttagcgggg cccttacatc atttttaact ggaattactg aaccagttga atatatgttt
    21901 ttatttgttg ctccttggct ttatgtgatt catgcattct tagatggatt atctttttat
    21961 tttgctgata tcttaaatat tcgtgtaggg aattcctttt caggtggttt gatcgacttt
    22021 ttattattcg gtgtacttca agggaatgac aaaactaatt ggttaaaatt aattcctttt
    22081 ggtattgcat gggcatttat atatttcttt gtttttacat tctttatcaa aaaatttaag
    22141 gtagctattc ctggaatgga agaagaatca gaagttcatt ctgaaaaagt aataaacaaa
    22201 aatacatcat tacatgaaga gtctcttatc attattgaag ctcttggtgg aaaagaaaat
    22261 attgagtctg ttgaagcttg tgcaactcgt ttgcgtgttg ctgttacgaa tggggagatt
    22321 attcaaaaac aaacaataaa agacactggt gcaactgctg tcttcgaagt gaagggtggc
    22381 atccaagctg tttttggagg gaaagctgac ttattaagtc aagaaatcaa tcaaatatta
    22441 ggaaaagata attaggaggt ttttaatatg tttgaaattt tcaaaaagaa aaaacaagaa
    22501 actttttatg caccttgttc cggaaaaatc atttccatca cagaagtaaa tgatcctgtc
    22561 ttttcacaaa aattactagg tgatggtttt gccgtcatac ctgatggttc aaaaatttat
    22621 gctccattgg ctggtcgagt aacaaatata tttcctacta aacatgctat taatataaaa
    22681 agcaactcag gcattgatta tttgattcat attggaattg atacagttga attacaagga
    22741 gagccttttt cgatttgtgt tcaagaaaat gatgaggtta ctcctgaaac ctcgttggtt
    22801 gatgtggatt ttgacaaaat aataaaatct ggtaaagata cttctgttat agttgttttt
    22861 ccggagaaaa atcagttcaa tgatttaata atagataatg gtcgaattgc tcatgggaat
    22921 tcatgtggaa aaatcacttt ttaaaaaagt gtatataaaa agctataaaa taagctaaat
    22981 aggatatttg tcaaatgaag ttggtgaaca gaaagaactg ttttctttct gttcattttt
    23041 ttattttatt agtaatgatt ccccatgcca aatcatgatc cgtaatttaa agttataaaa
    23101 gcttcgaaat ccattggcat ttcgttggaa taccttgata tgattattca aatataaaat
    23161 atagagagaa gagagagagg gaagatattt ataaagggca aagttatgtt tactcaactt
    23221 aggaaataga aatagatcaa tttatattct cttgtcaatc gaatatgaac cttagcacta
    23281 tataccaaat atagtaaaat gctgaaagat g
//
LOCUS       DARDHA010000024        23251 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000024 DARDHA010000000
VERSION     DARDHA010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.024"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            68..751
                     /locus_tag="SDA10_000815"
     CDS             68..751
                     /locus_tag="SDA10_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEG4239743.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="SDA10_000816"
     CDS             753..1589
                     /locus_tag="SDA10_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG4239744.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="SDA10_000817"
     CDS             1600..3354
                     /locus_tag="SDA10_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4239745.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="SDA10_000818"
     CDS             complement(3630..3971)
                     /locus_tag="SDA10_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG4239746.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="SDA10_000819"
     CDS             complement(4039..6210)
                     /locus_tag="SDA10_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEG4239747.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6379..7278
                     /locus_tag="SDA10_000820"
     CDS             6379..7278
                     /locus_tag="SDA10_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG4239748.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="SDA10_000821"
     CDS             complement(7349..8206)
                     /locus_tag="SDA10_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG4239749.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="SDA10_000822"
     CDS             complement(8191..10893)
                     /locus_tag="SDA10_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEG4239750.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="SDA10_000823"
     CDS             complement(10906..12195)
                     /locus_tag="SDA10_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEG4239751.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12345..13391
                     /locus_tag="SDA10_000824"
     CDS             12345..13391
                     /locus_tag="SDA10_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4239752.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13393..15546
                     /locus_tag="SDA10_000825"
     CDS             13393..15546
                     /locus_tag="SDA10_000825"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEG4239753.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(16019..17470)
                     /locus_tag="SDA10_000826"
     CDS             complement(16019..17470)
                     /locus_tag="SDA10_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4239754.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(17467..19191)
                     /locus_tag="SDA10_000827"
     CDS             complement(17467..19191)
                     /locus_tag="SDA10_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG4239755.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(19184..19798)
                     /locus_tag="SDA10_000828"
     CDS             complement(19184..19798)
                     /locus_tag="SDA10_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG4239756.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(20143..21600)
                     /locus_tag="SDA10_000829"
     CDS             complement(20143..21600)
                     /locus_tag="SDA10_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4239757.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(21641..22585)
                     /locus_tag="SDA10_000830"
     CDS             complement(21641..22585)
                     /locus_tag="SDA10_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4239758.1"
                     /translation="MHYSKEEKNEKKKVERVQVRSFNKTANFFSNIMIALFAISCILP
                     FIFVIAISFTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGT
                     LINSTATSLYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWA
                     LILPMAVSPFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATIS
                     LFAALGYWNDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVP
                     GESARMAIVVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(22572..>23251)
                     /locus_tag="SDA10_000831"
     CDS             complement(22572..>23251)
                     /locus_tag="SDA10_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4239759.1"
                     /translation="NVAFILLNLLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLSWV
                     IISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSIIY
                     FASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGLFY
                     NIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARRF
                     DSDSALF"
BASE COUNT         6668 a         4784 c         4157 g         7642 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa agattatact
    12241 tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact ttttcatcca
    12301 ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgact caaccattgt
    12361 accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta cctgtagaca
    12421 gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga atcacttcca
    12481 aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc gggaaaggca
    12541 gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa acacgacgga
    12601 ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc aagggattga
    12661 accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac tttctacaaa
    12721 ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat gctcttgata
    12781 cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct gtcgtcatat
    12841 tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat ttccaaggcg
    12901 gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct tatttgtttg
    12961 gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt gaagcattgg
    13021 acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca ttagatgaat
    13081 tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac gatgttgtcg
    13141 ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa gatttttcaa
    13201 ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg acaaccatcg
    13261 cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact tggttagaga
    13321 caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga caatcaacaa
    13381 aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc gaatcagcca
    13441 aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat gaattatttt
    13501 gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga ccgcgttttt
    13561 caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag tcatatgctg
    13621 tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca aagttcttac
    13681 cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt acgttatggc
    13741 atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga ttacagcaaa
    13801 aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt tatagatcct
    13861 tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga tttttctttt
    13921 tattttatat tgagatttga atatccttta acaattgatg agttagctgt ttccaacaaa
    13981 aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat ccgatttggg
    14041 acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca aaaccaaagc
    14101 aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga tcgtatacag
    14161 ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata tcgggtgttt
    14221 ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca ctatgatacg
    14281 actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg ggatacgtat
    14341 aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga aatgctggaa
    14401 ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc tcctgatgaa
    14461 cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc tgtaaaagat
    14521 atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc tacggttcaa
    14581 agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata tggctatgtt
    14641 cctaatcact accacgaatc agtcaatcac acattagact atgcttatag cgatttttgt
    14701 attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata tcagcagcaa
    14761 tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc aaaagataaa
    14821 gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta tgcagaagga
    14881 agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat cggaaaatat
    14941 ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc cccaacgttt
    15001 gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc gatagaattt
    15061 ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt cagttatata
    15121 ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac cttcgacgat
    15181 tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg gtatatcttc
    15241 aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat cggtatgcca
    15301 ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat tgaagcaacg
    15361 cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacag agaatatacg
    15421 aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta ccaattaggg
    15481 atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt atcaaactat
    15541 aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa
    15601 aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa gcttttgaac
    15661 accgtttatt gagaggttgt gaaatcagcg ttctgacctg agtattagag agacaaatgg
    15721 caaaatagtt cctcatattt tgccattttt tgagctaata ccgagggtca gctgattgaa
    15781 caccgtttag tggagaggtc gtgaaaaagc tgtttagttt cgagtattaa gaaaaatttt
    15841 gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgagaa actggctttt
    15901 gaactccgtt tatcgagagg tcatgaaaaa gctgccttgc ctcaagtaat aagaacagcc
    15961 caaaattaga tttctagaca attttttata agttatttca ttacattggc tttttctttt
    16021 atgattctcc actgttttct cggtactctt taggggatct gccgacgatt ttcttgaata
    16081 atttagagaa atacgtggtg ttgttgtagc caatctcact tgctatctca ttgatgtttt
    16141 gattactgtg caaaagcagt tgtttcgctt tttccatacg taaatggttc aaataagctg
    16201 aaaaactttt tttcgtttct tttttgaaca gctgtcctag atacatgaca tttaaatgca
    16261 ggttttctga cacttctttg agtgtaagtg gttcttgata atgctggtgc aggattacga
    16321 ttactttttc cacattgtca ctgtactttt tctgtctctg atgctttttg atgaatgaca
    16381 gaagtagctg ctgaagttct tgcaccgttt ttgcctgatg gattttttcg attccttgta
    16441 aatattcctc atcttccaga tcaatcaatt cccgttgaat ctccataaac agtaaaaaac
    16501 tggaatatcg tatatcttcc ggcatcatgg ctgctaactg aaactgttca aaaaaatcag
    16561 tgatcatttt ttgagcgact tccaattgtt tattttgcag cacgcgtctg aaacttgaaa
    16621 aatcgatggt ttgatctttt gtttctgctt gtactgcgta aaaaacatgc tcgtctttat
    16681 ctccataaaa ttgatgcaac tgtagattgt ctttcgcttg ttggaatgtt tcagggattt
    16741 tgtccaaaga atctgtctct tccccgatac tgatcagcca ttcctgatca ggaaagtttt
    16801 cttgaacaaa acaacagaag ccatcaactt catctgcctc caacaacaac gtgatcaata
    16861 cacggtctcc gtaatttcgt tgatagtaaa agatttgttg ccgttcttgc aaccatctat
    16921 tgataagagt atcatgagtt cttgccagtt gaatcaatac tatcctatgc tgcttctcac
    16981 ttatttgtcc tattatttct tcttcactta tttcatccaa ttcttcattc aaccattgac
    17041 tgaataacag ttcttgatag atttcttgtt gatttttttg ttcattttgc tgatctaacc
    17101 gtgttttgat tttcttgagg ctttcaacca attcgaactt attcacgggt ttcatcaaat
    17161 aattcacggc gcctaattgc atacctcctt tcaaataatc gaattcttgg tatcctgata
    17221 aaatcataaa ttcaaaatta tgacgttctt tttgagccgc ttcaataaat tccagtccat
    17281 ttatttctgg cattgtcaca tccgttacta ctaaatcaat ctgtcgctgt tcaagtacag
    17341 ctagtccttc tttggcattc tctgctgttg ctactatttg aaagcctaaa ctttgccaat
    17401 caattatctt ttttaaccca ttcaaaatca tgtattcatc atctaccaat aacacttgat
    17461 acacgctcat ccctccttga atgtaataga aacggtcagt ccacctgtct cattattctc
    17521 aagcgacata taataactgc tgccgaagct ggcacgtaaa cgttcattca cgttttgcag
    17581 accgatagaa ccgtgaagtt ccactttagg atgagacagt ttttcttcaa tctgttttag
    17641 ccgtttttca gtcatccctt ttccattatc tttgatgatg atccttaccc gtttgccttc
    17701 ctgcaagact ttgacactta gcgcattatc gaatcgagta aaatcaatac cgtgtttaaa
    17761 ataattttca actaatggct ggatcacaaa tttaggtact tcgatctttt ccaaatcagg
    17821 atcgatcata aaatgatagg ctactcgatt aggataacgc atttgataca aatagacata
    17881 tttttcgcaa aagtccagtt cttcttttaa agtgatcgtt ttctcttgat tcgtattgtt
    17941 tcgaagcaaa gcggaaaacg catacacaac atctgctaat tcttcacttc cttcgctgat
    18001 tgcatacatt cggatatatt ctaatgtatt atataaaaag tgtggattga tctgtgcttg
    18061 aagcgcccgc atatgtgcgt cttgctgttt gatttctaat ttgtagatgt cttctacaaa
    18121 ctgattgatg ctatccaaca tttcgttgat accgattgaa agttccctca gttcatattc
    18181 cgtgtttgtt atgtcgatcc gcgtctctaa attccctgct gctgtttgat ccatggcctg
    18241 catgacgata gatacttgct gtgaatatcg tttgaatgtt ttataaagaa agataagcag
    18301 tatattgtct aaaataatga taccaatgat cagcggggca agattttttc ccgtttcgat
    18361 ttttacactt tttttatcta gtacagctaa tatacgataa tcatcagaca gttcttggtg
    18421 ttgaacaaaa ttgttttttt ccagatcgtc aatcggcaat tgtgaatttt tcttcagact
    18481 ctctgtgatg atcatttctt gtctttcaaa cgtttcttta gctattttat tatcatggaa
    18541 tgtgtatagt cgatttgtcg ttccagaaat catgtataaa gagagtccat tgaatgacgt
    18601 tagattttct aaaatcatat ctagttcttt cttttgaaaa ccaataaaaa tacttcctga
    18661 ctcagctcct ccttgtgtga taggcaactt gatataaaag gcgtctagta attttggtgt
    18721 cccctttttt atttccccac ctttgttttc tctagaagat tcataatact ccggtagttg
    18781 attcaacacg atatatatcg cacttagttc ttcataattt gcgtagagat ctttgatttg
    18841 atttgggaaa gaaactaatt catcggtact gttttgctgt tcgtatacat acatcagata
    18901 ctgatctatg ggttttgtta agtaggtcgt cacattttcg atcttttcgg aattccctac
    18961 aagctggttt ttgatgatcc tactcaaggt tgttttatca ttcagcattc tgcttgtgct
    19021 ttgaacggca tcacttgtca tgttttctgc ctgtttttga ttttgttccc aagtatggat
    19081 actaaaaata acggtagcag tcatcgttac accgatcatg ataaccgcgt atttccgcaa
    19141 cagacgattt agtaaggttc cttttgaaaa tagaatccga aacttattca taactttcca
    19201 actgtttatc taattcttca cgccaatgtg ttgtcatcgt accattccaa accgtcaaaa
    19261 gaccaatgac accaaacagg atcaatcctt tgaattgcca agtcacgcct aaaattatac
    19321 cgctaccaat aattattttt agaaaagacg aaaagctgga aaaaaccgaa ataaaactta
    19381 gctttagcag attaggaaca gagatcgtat attcactatc taaaatcata ctgtattgat
    19441 aagtagcaaa taggcagagc aaggcaaaaa gtaaaataaa atcaatagct aaaaaaatga
    19501 gccctttgat ttgaacagat aaatataaat tgtaactcaa taaagcagta ccagataaaa
    19561 ataaactaaa tagcagattg cctcttaaaa aatttctttt atatatattc cagcctcttt
    19621 ttattgtgat ttctttatac tcgaatccgt acaaataaaa tagttcattg actgttttcc
    19681 atgccgcacc taatccaaag atgatcccgc cggcaaatgt atatatccag aaatacaagg
    19741 ttagttttat aatgacccag atccggataa ataactcttc caaaaccttc cctaacatat
    19801 cttctcctcc aaacattaag tttatacttt ctcattatac aaaaaaaggt tgggcaatca
    19861 gcaccttgac ctgattatta gagaaacaaa cggaaaaata gttcctcata ttttgtcgtt
    19921 ttgtgagcga ataccgaggg ttagctgatt gaacaccgtt taccaagagt ttgtgaaata
    19981 gccgcttagt ttcgagtagt aagaaaaatt ttgtgaatat ggctttccac atttttacat
    20041 atatttagct taataccgag aaactggctt ttgaacaccg tttattcgga acaagaggtt
    20101 gtgacacgtc ttttgtcaca acccctttgt ctttattcat tattattctt gtcggaactc
    20161 atcatattgt ttttgcattt ctttcaatac cttatcatag ccagccttgt ccaaagcgtc
    20221 tttcaatttc ggtaatgttt catctggatc aacagtccct gtattcaatc catctaaata
    20281 ttgattcatg acattagaaa tattacttaa ttctgttttg acactgtcag tgttgaagct
    20341 gaatcctaaa atcggtgaag tttctgcatc agcaatcgat tgatcacgtt tggcaatcat
    20401 gtcatccgta atagactctt gtgtatatag aatcttgttg tttcctgtat tccaagcaga
    20461 catatgtgta tttggctgat aaccatctag caatttgatt ttatcatctg aaccatcgac
    20521 tttttcccat gcttctccct cgatccccca aacaagccca ttcaataatt ctggatcact
    20581 gttaagcagc cctagaagtt ctactgattt ttctttattt tttgaagtat ttgatacgac
    20641 aaaatttgcc atttgtgctt gagctgtaga ttttagtgga acagtaattg cttttgagac
    20701 aagttcttgt tttgctgcat ttgtcaaaat tgtatcgcca taatcatacg gtccttgtgt
    20761 ttctccacgc attagccaag tatttccttc tagtggataa tccttattgc tcgttgcagc
    20821 gtcacttgga atgagtcctt gtttgtacca gctatgcatt gtacgtaaca gtccgatata
    20881 cgtttcatta tcgtattggt tgatgatttt cgaatcatct ccatccagat caatagcaaa
    20941 tggtaacgta ttccctaaag gataatcaaa gttcccttgg actttgaagc cttgtccaat
    21001 ggcaaaagct gccgtgtttg gttctttttc atggaattcc ttcaagactt tttctgcatc
    21061 agcgtaagat tggattccat caattgaaag atcgtattta tctagtaaag ctttgttaaa
    21121 agtaagtact tgttgagcaa agacatttcc attgactggg aaagcatata attttccatt
    21181 gaccagattt cctttaatat atgcttcatc taaatcatta taagcttttt cagcgtactt
    21241 aggcgctaat tcagtcaaat cagcaaaggc tcctttttgg gcattggaaa catagttgtt
    21301 cgcaaaggcg atatcataat tttccccgga agaaatgatg acgttcattt tcttttcgta
    21361 atcgccccag ccaatgtaat tgatattgac ttttacaccg atctcttttt cgattcgttt
    21421 gtttgctact tccatcaatt gatcgaaatt atctggttta tctccgattt gatacatctg
    21481 caaagtgtcc gaattgctag cttctgcctg gtctttagaa ccagaacctc cgcatgctgc
    21541 taatgttccc aacaacaacg ctgaaacacc tgtaaaggct agtttcttcc ataacttcat
    21601 ttttcttcct cctaatttta tgatttatat ttttgtttct ttattctttt actccgccga
    21661 ttgttagacc tttgacaaaa tgcttctgga aaaatggata gctgatcgca ataggcaacg
    21721 tcgagatgac tacgatcgcc attctggcag attctcctgg aactgaggct aatccgcctg
    21781 tcaattggct tcctgctcct gaattttgtg tcagatattg aatattactt tggattttca
    21841 tcaaaagata ctgtagtgga actagcttat catcttggat gtatagtaaa gcattgaacc
    21901 agtcattcca gtaacccaat gcggcaaata aactgattgt cgcaatacct ggaacagcta
    21961 acggtaaaac gatctgaaca aatattcgca gttcgctagc tccatcgatc cgtgccgatt
    22021 caataatcga atcagagacc gaacgtttaa agaatgtgcg catcacaata atattgaagg
    22081 gactgacagc cattggcaag atcaatgccc aaatcgtatc ttttaactga agaagatttg
    22141 tcataacaag ataattggcc accatccctg gtgagaacaa catcgtaatc aaacaaaaaa
    22201 cggtaaaaaa acgacggaat ggaaagtttg accgtgaaat cgcataagca tataaagaag
    22261 tagccgtgct attgatcagt gtacctaaaa cagtaacaag tacagtgacg aaaagcgctt
    22321 ggaaaatttt atcctgcatc tggtcgaaca agtacgtata accaaatata ctgaactttt
    22381 ctggccaaaa actgtacccg tgagtcgaca atatggactc atcggtaaaa gagatggcga
    22441 taacaaatat aaatggcaaa atacaggaaa tagcaaagag tgcaatcatt atattagaga
    22501 aaaaattagc cgtcttattg aaagagcgga cttggactct ctccactttc tttttttcat
    22561 ttttttcctc cttagaataa tgcagaatcg ctatcaaaac gtcgggcgat cgtatttgtg
    22621 atcatcaaca agacaaagcc gacaactgat tggtaaagtc ctgcagcagc cgtcatacca
    22681 aaatctcctg tggatgtcag gccattgtag atatacgtat ccaaaacttg agtcacatca
    22741 tataatgcac cggaattcct aggaatgtta taaaacaggc caaaatcagc tcggaaaatg
    22801 tttcctacgg ctaaaatcgt taaaatcgtc attagtggaa taagttgtgg tatcgtcaca
    22861 tgtttgatct gctgccattt gctagctcca tcaaccatag cagcttcata ataagtcgga
    22921 tcgattccca taacagatgc aaagtaaata atgctgttat aaccaatccc tttccatgta
    22981 ccaatgaaca ccaagataaa cggccaatat ttcggttcat tataccaatt gacagcttct
    23041 cctccatgtc cagtgatcca ctgattgaat attcctttgt caggacttaa aaaagcgtaa
    23101 acaaagtaac tgataatgac ccaagataga aaataaggta gtaaggacat cgtttgataa
    23161 actttaacga gtcgtttatt tctcaactcg ctcataatga ttgcaaatac gactgagatc
    23221 agcagattca aaaggataaa tgctacatta t
//
LOCUS       DARDHA010000025        23104 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000025 DARDHA010000000
VERSION     DARDHA010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.025"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            363..731
                     /locus_tag="SDA10_000832"
     CDS             363..731
                     /locus_tag="SDA10_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEG4239760.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="SDA10_000833"
     CDS             911..1852
                     /locus_tag="SDA10_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEG4239761.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="SDA10_000834"
     CDS             1921..2652
                     /locus_tag="SDA10_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEG4239762.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="SDA10_000835"
     CDS             2796..4976
                     /locus_tag="SDA10_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEG4239763.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="SDA10_000836"
     CDS             4996..5478
                     /locus_tag="SDA10_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEG4239764.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="SDA10_000837"
     CDS             5494..6255
                     /locus_tag="SDA10_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEG4239765.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /locus_tag="SDA10_000838"
     CDS             6386..7720
                     /locus_tag="SDA10_000838"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEG4239766.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="SDA10_000839"
     CDS             8027..8512
                     /locus_tag="SDA10_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HEG4239767.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="SDA10_000840"
     CDS             8505..9188
                     /locus_tag="SDA10_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEG4239768.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="SDA10_000841"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="SDA10_000841"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEG4239769.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="SDA10_000842"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="SDA10_000842"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEG4239770.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12804..13433
                     /locus_tag="SDA10_000843"
     CDS             12804..13433
                     /locus_tag="SDA10_000843"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEG4239771.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="SDA10_000844"
     CDS             13485..13817
                     /locus_tag="SDA10_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEG4239772.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14129..14620
                     /gene="nrdR"
                     /locus_tag="SDA10_000845"
     CDS             14129..14620
                     /gene="nrdR"
                     /locus_tag="SDA10_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEG4239773.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14643..16016
                     /locus_tag="SDA10_000846"
     CDS             14643..16016
                     /locus_tag="SDA10_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEG4239774.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16016..16942
                     /gene="dnaI"
                     /locus_tag="SDA10_000847"
     CDS             16016..16942
                     /gene="dnaI"
                     /locus_tag="SDA10_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEG4239775.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17071..17511
                     /locus_tag="SDA10_000848"
     CDS             17071..17511
                     /locus_tag="SDA10_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEG4239776.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17655..18356
                     /locus_tag="SDA10_000849"
     CDS             17655..18356
                     /locus_tag="SDA10_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEG4239777.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18353..19474
                     /locus_tag="SDA10_000850"
     CDS             18353..19474
                     /locus_tag="SDA10_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEG4239778.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19489..20721
                     /gene="pepT"
                     /locus_tag="SDA10_000851"
     CDS             19489..20721
                     /gene="pepT"
                     /locus_tag="SDA10_000851"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEG4239779.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20853..21761
                     /locus_tag="SDA10_000852"
     CDS             20853..21761
                     /locus_tag="SDA10_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEG4239780.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21796..22068
                     /locus_tag="SDA10_000853"
     CDS             21796..22068
                     /locus_tag="SDA10_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEG4239781.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            22079..>23104
                     /locus_tag="SDA10_000854"
     CDS             22079..>23104
                     /locus_tag="SDA10_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEG4239782.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKE"
BASE COUNT         7777 a         4029 c         5069 g         6229 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggcaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataatattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaatacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13861 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13921 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13981 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14041 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14101 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14161 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14221 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14281 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14341 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14401 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14461 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14521 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14581 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14641 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14701 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14761 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14821 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14881 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14941 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15001 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15061 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15121 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15181 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15241 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15301 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15361 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15421 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15481 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15541 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15601 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15661 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15721 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15781 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15841 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15901 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15961 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16021 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16081 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16141 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16201 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16261 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16321 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16381 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16441 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16501 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16561 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16621 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16681 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16741 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16801 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16861 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16921 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16981 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17041 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17101 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17161 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17221 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    17281 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17341 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17401 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17461 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17521 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17581 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17641 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17701 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17761 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17821 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17881 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17941 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    18001 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    18061 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    18121 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    18181 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    18241 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    18301 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18361 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18421 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18481 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18541 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18601 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18661 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18721 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18781 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18841 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18901 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18961 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    19021 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    19081 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    19141 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    19201 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    19261 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    19321 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19381 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19441 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19501 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19561 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19621 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19681 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19741 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19801 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19861 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19921 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19981 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    20041 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    20101 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    20161 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    20221 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    20281 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    20341 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20401 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20461 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20521 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20581 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20641 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20701 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20761 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20821 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20881 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20941 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    21001 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    21061 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    21121 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    21181 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    21241 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    21301 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21361 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21421 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21481 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21541 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21601 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21661 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21721 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21781 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21841 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21901 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21961 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    22021 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    22081 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    22141 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    22201 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    22261 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    22321 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22381 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22441 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22501 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22561 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22621 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22681 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22741 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22801 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22861 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22921 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22981 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    23041 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    23101 agag
//
LOCUS       DARDHA010000026        23063 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000026 DARDHA010000000
VERSION     DARDHA010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.026"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(109..564)
                     /locus_tag="SDA10_000855"
     CDS             complement(109..564)
                     /locus_tag="SDA10_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239783.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="SDA10_000856"
     CDS             complement(718..1926)
                     /locus_tag="SDA10_000856"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEG4239784.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="SDA10_000857"
     CDS             complement(2038..2370)
                     /locus_tag="SDA10_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEG4239785.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="SDA10_000858"
     CDS             2518..3393
                     /locus_tag="SDA10_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG4239786.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="SDA10_000859"
     CDS             complement(3475..4056)
                     /locus_tag="SDA10_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEG4239787.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="SDA10_000860"
     CDS             complement(4062..5321)
                     /locus_tag="SDA10_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEG4239788.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="SDA10_000861"
     CDS             complement(5323..6369)
                     /locus_tag="SDA10_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239789.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="SDA10_000862"
     CDS             complement(6604..6879)
                     /locus_tag="SDA10_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEG4239790.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /gene="der"
                     /locus_tag="SDA10_000863"
     CDS             complement(7122..8432)
                     /gene="der"
                     /locus_tag="SDA10_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEG4239791.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="SDA10_000864"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="SDA10_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEG4239792.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /locus_tag="SDA10_000865"
     CDS             complement(9978..10658)
                     /locus_tag="SDA10_000865"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEG4239793.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="SDA10_000866"
     CDS             complement(10707..11342)
                     /locus_tag="SDA10_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG4239794.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="SDA10_000867"
     CDS             complement(11408..12811)
                     /locus_tag="SDA10_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEG4239795.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="SDA10_000868"
     CDS             complement(12798..13829)
                     /locus_tag="SDA10_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4239796.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="SDA10_000869"
     CDS             13860..14081
                     /locus_tag="SDA10_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEG4239797.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="SDA10_000870"
     CDS             complement(14246..14983)
                     /locus_tag="SDA10_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEG4239798.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="SDA10_000871"
     CDS             complement(15136..16476)
                     /locus_tag="SDA10_000871"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HEG4239799.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="SDA10_000872"
     CDS             complement(16713..18236)
                     /locus_tag="SDA10_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEG4239800.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18614..19225)
                     /locus_tag="SDA10_000873"
     CDS             complement(18614..19225)
                     /locus_tag="SDA10_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG4239801.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19348..19473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19555..20271)
                     /locus_tag="SDA10_000874"
     CDS             complement(19555..20271)
                     /locus_tag="SDA10_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEG4239802.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="SDA10_000875"
     CDS             complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="SDA10_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEG4239803.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20830..21627)
                     /locus_tag="SDA10_000876"
     CDS             complement(20830..21627)
                     /locus_tag="SDA10_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEG4239804.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21597..21968)
                     /locus_tag="SDA10_000877"
     CDS             complement(21597..21968)
                     /locus_tag="SDA10_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEG4239805.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="SDA10_000878"
     CDS             complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="SDA10_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEG4239806.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6466 a         4926 c         3910 g         7761 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccc ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    18301 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    18361 tttccctatt ccctcctctt ttttcagttt tttaaagctt tcttcttttt cattgtagcg
    18421 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    18481 caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa
    18541 cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg
    18601 atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac
    18661 ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt
    18721 tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca
    18781 tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg
    18841 cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc
    18901 ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg
    18961 ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca
    19021 gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca
    19081 tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga
    19141 tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca
    19201 ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa
    19261 ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag
    19321 ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt
    19381 atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg
    19441 gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca
    19501 ttatacatat ctattcaaaa aacgcaacat ctaacaatta taaaaagtaa gtgttcattt
    19561 tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg
    19621 taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc
    19681 aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac
    19741 gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt
    19801 tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc
    19861 ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc
    19921 aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac
    19981 aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc
    20041 atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc
    20101 ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa
    20161 ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt
    20221 tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat
    20281 tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt
    20341 tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag
    20401 tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca
    20461 atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc
    20521 tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg
    20581 gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct
    20641 tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag
    20701 ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga
    20761 tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg
    20821 gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat
    20881 tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa
    20941 agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt
    21001 acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt
    21061 tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat
    21121 tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc
    21181 agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc
    21241 tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt
    21301 gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtgc cgaattcttg
    21361 gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc
    21421 tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt
    21481 gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag
    21541 cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac
    21601 tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca
    21661 cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc
    21721 tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc
    21781 cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt
    21841 tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc
    21901 agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt
    21961 gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc
    22021 gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct
    22081 aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta
    22141 tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc
    22201 agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc
    22261 atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat
    22321 tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt
    22381 accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc
    22441 atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc
    22501 gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc
    22561 tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc
    22621 cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct
    22681 tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc
    22741 catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag
    22801 ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg
    22861 atttgctctt tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt
    22921 ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact
    22981 ggtttctttt ttcggctcta taatttatgg gactgatgaa tcagaattga ttcttcagcc
    23041 ccattttcat tttaggtatt aaa
//
LOCUS       DARDHA010000027        22685 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000027 DARDHA010000000
VERSION     DARDHA010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.027"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            133..3660
                     /locus_tag="SDA10_000879"
     CDS             133..3660
                     /locus_tag="SDA10_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEG4239807.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="SDA10_000880"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="SDA10_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEG4239808.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="SDA10_000881"
     CDS             7448..7789
                     /locus_tag="SDA10_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4239809.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="SDA10_000882"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="SDA10_000882"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEG4239810.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="SDA10_000883"
     CDS             9146..11566
                     /locus_tag="SDA10_000883"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEG4239811.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="SDA10_000884"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="SDA10_000884"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEG4239812.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="SDA10_000885"
     CDS             13000..13737
                     /locus_tag="SDA10_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG4239813.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="SDA10_000886"
     CDS             13751..14566
                     /locus_tag="SDA10_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEG4239814.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="SDA10_000887"
     CDS             14579..15226
                     /locus_tag="SDA10_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG4239815.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="SDA10_000888"
     CDS             15242..15898
                     /locus_tag="SDA10_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG4239816.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="SDA10_000889"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="SDA10_000889"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEG4239817.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="SDA10_000890"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="SDA10_000890"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEG4239818.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="SDA10_000891"
     CDS             18240..18758
                     /locus_tag="SDA10_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG4239819.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="SDA10_000892"
     CDS             18865..19365
                     /locus_tag="SDA10_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG4239820.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="SDA10_000893"
     CDS             complement(19645..20565)
                     /locus_tag="SDA10_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEG4239821.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="SDA10_000894"
     CDS             20730..21743
                     /locus_tag="SDA10_000894"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEG4239822.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="SDA10_000895"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="SDA10_000895"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEG4239823.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         7560 a         3953 c         4828 g         6344 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttga
//
LOCUS       DARDHA010000028        21988 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000028 DARDHA010000000
VERSION     DARDHA010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.028"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..395
                     /locus_tag="SDA10_000896"
     CDS             <1..395
                     /locus_tag="SDA10_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239824.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="SDA10_000897"
     CDS             398..1147
                     /locus_tag="SDA10_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4239825.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="SDA10_000898"
     CDS             1159..1428
                     /locus_tag="SDA10_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239826.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="SDA10_000899"
     CDS             1433..1597
                     /locus_tag="SDA10_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239827.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="SDA10_000900"
     CDS             1590..1892
                     /locus_tag="SDA10_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239828.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="SDA10_000901"
     CDS             1889..2050
                     /locus_tag="SDA10_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG4239829.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="SDA10_000902"
     CDS             2047..2352
                     /locus_tag="SDA10_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239830.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="SDA10_000903"
     CDS             2352..2708
                     /locus_tag="SDA10_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HEG4239831.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2695..2913
                     /locus_tag="SDA10_000904"
     CDS             2695..2913
                     /locus_tag="SDA10_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239832.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="SDA10_000905"
     CDS             2910..3329
                     /locus_tag="SDA10_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HEG4239833.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="SDA10_000906"
     CDS             3326..3883
                     /locus_tag="SDA10_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEG4239834.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="SDA10_000907"
     CDS             3880..4176
                     /locus_tag="SDA10_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239835.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="SDA10_000908"
     CDS             4253..4666
                     /locus_tag="SDA10_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEG4239836.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="SDA10_000909"
     CDS             5012..5386
                     /locus_tag="SDA10_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239837.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="SDA10_000910"
     CDS             5423..5590
                     /locus_tag="SDA10_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239838.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="SDA10_000911"
     CDS             5616..5960
                     /locus_tag="SDA10_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG4239839.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="SDA10_000912"
     CDS             5965..6246
                     /locus_tag="SDA10_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239840.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="SDA10_000913"
     CDS             6349..6663
                     /locus_tag="SDA10_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEG4239841.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="SDA10_000914"
     CDS             6641..8335
                     /locus_tag="SDA10_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG4239842.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="SDA10_000915"
     CDS             8355..9533
                     /locus_tag="SDA10_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG4239843.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="SDA10_000916"
     CDS             9496..10182
                     /locus_tag="SDA10_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEG4239844.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="SDA10_000917"
     CDS             10182..11342
                     /locus_tag="SDA10_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG4239845.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="SDA10_000918"
     CDS             11352..12227
                     /locus_tag="SDA10_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239846.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="SDA10_000919"
     CDS             12224..12535
                     /locus_tag="SDA10_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239847.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="SDA10_000920"
     CDS             12525..12878
                     /locus_tag="SDA10_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEG4239848.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="SDA10_000921"
     CDS             12868..13269
                     /locus_tag="SDA10_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239849.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="SDA10_000922"
     CDS             13262..13666
                     /locus_tag="SDA10_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239850.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="SDA10_000923"
     CDS             13678..14286
                     /locus_tag="SDA10_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG4239851.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="SDA10_000924"
     CDS             14306..14668
                     /locus_tag="SDA10_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239852.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="SDA10_000925"
     CDS             14671..14853
                     /locus_tag="SDA10_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239853.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="SDA10_000926"
     CDS             14870..18289
                     /locus_tag="SDA10_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEG4239854.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="SDA10_000927"
     CDS             18340..19077
                     /locus_tag="SDA10_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG4239855.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="SDA10_000928"
     CDS             19087..21378
                     /locus_tag="SDA10_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG4239856.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="SDA10_000929"
     CDS             21402..>21988
                     /locus_tag="SDA10_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239857.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3245 c         4705 g         6105 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatttg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DARDHA010000029        21960 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000029 DARDHA010000000
VERSION     DARDHA010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.029"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1305)
                     /locus_tag="SDA10_000930"
     CDS             complement(<1..1305)
                     /locus_tag="SDA10_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEG4239858.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDN"
     gene            complement(1317..2735)
                     /locus_tag="SDA10_000931"
     CDS             complement(1317..2735)
                     /locus_tag="SDA10_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4239859.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(2975..4543)
                     /locus_tag="SDA10_000932"
     CDS             complement(2975..4543)
                     /locus_tag="SDA10_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEG4239860.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4547..4828)
                     /locus_tag="SDA10_000933"
     CDS             complement(4547..4828)
                     /locus_tag="SDA10_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEG4239861.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4825..5748)
                     /locus_tag="SDA10_000934"
     CDS             complement(4825..5748)
                     /locus_tag="SDA10_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HEG4239862.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5745..6623)
                     /locus_tag="SDA10_000935"
     CDS             complement(5745..6623)
                     /locus_tag="SDA10_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEG4239863.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6610..7947)
                     /locus_tag="SDA10_000936"
     CDS             complement(6610..7947)
                     /locus_tag="SDA10_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEG4239864.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(7950..9053)
                     /locus_tag="SDA10_000937"
     CDS             complement(7950..9053)
                     /locus_tag="SDA10_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG4239865.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9127..10635)
                     /locus_tag="SDA10_000938"
     CDS             complement(9127..10635)
                     /locus_tag="SDA10_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG4239866.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10601..11293)
                     /locus_tag="SDA10_000939"
     CDS             complement(10601..11293)
                     /locus_tag="SDA10_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG4239867.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11315..12289)
                     /locus_tag="SDA10_000940"
     CDS             complement(11315..12289)
                     /locus_tag="SDA10_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4239868.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12589..13461
                     /locus_tag="SDA10_000941"
     CDS             12589..13461
                     /locus_tag="SDA10_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4239869.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14043..16241)
                     /locus_tag="SDA10_000942"
     CDS             complement(14043..16241)
                     /locus_tag="SDA10_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4239870.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16473..17465
                     /locus_tag="SDA10_000943"
     CDS             16473..17465
                     /locus_tag="SDA10_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4239871.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17525..18946
                     /locus_tag="SDA10_000944"
     CDS             17525..18946
                     /locus_tag="SDA10_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEG4239872.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            18939..20438
                     /locus_tag="SDA10_000945"
     CDS             18939..20438
                     /locus_tag="SDA10_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEG4239873.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20477..21871
                     /gene="uxaC"
                     /locus_tag="SDA10_000946"
     CDS             20477..21871
                     /gene="uxaC"
                     /locus_tag="SDA10_000946"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEG4239874.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6639 a         4486 c         3817 g         7018 t
ORIGIN      
        1 attgtcaaaa gtattgtttg cagctgtgtt gccagtttct atagataaat cacagctttt
       61 gaaacataag ttttgtactg cttcgattcg cccgaattct tttgcagtta tttggcaatg
      121 agaaaagcag acatcttcta taggcttttc aggaaatgct actaaatcaa aagctcggga
      181 aggaagtgag taacctggag aaaggcatgc ctgtacgttt ttgatgtgta tgtctttgac
      241 tttcgtcatt tgctttgatt caggtatttc ttcagccact gcttgccaac tttctggcat
      301 ttgagcaagg tcattgctat tttttctatt gtattcatta tgccagtcca ttatccaaga
      361 aaaaggaaat tgtacatcaa tcatattcag attttctacc ctgatatttt cgatcactcc
      421 ccctctttct tttgaagatt tcattcgaat accacagtct gactgaaaaa aattgatatc
      481 gtgaatatat acgtcactaa ctccagcact aacttcgcta ccaatagtta caccgtatcc
      541 tgaacggatt ttacatctag caacctcgat tcttgatgat tttcgattga ctcttgcacc
      601 attgccatct ctgccagact taatagcaat acaatcatca ccacaggcca aatcacattc
      661 atagacacgc acatttgttg aagaatcaat atcaatccca tctgtacttg gtccatcatt
      721 gttgcggatg attacttttt ctactaatac atcgttagaa taagtgattt gcaagttcca
      781 aaaacctgat ttctgaaaaa tcaaatcacg aacaacaaca tgttctgcat gatacagtaa
      841 acaagcgcgt ggacgtttaa tagcataatc cgcaatccaa cgaagtccct tttgatcgta
      901 ttcagctctt gtcccgcttt tttgatcttt tccccaatac agctcccacc aatggtctcc
      961 ttgaccgtcg attattcctt ttccttctat aagaatatct ttcgccgaga gaacattcaa
     1021 aatcgctgct ggccatttca tttctacgcc ggctactcgt gtatcgattt ctggaaatgc
     1081 ttcgatatct gttctcccct tgaggactgc accttcttca aagataagat gactcttatc
     1141 ttttaaaaaa agggctccga ctaaataaat gccttttgga ataaatacag gtaagtgttc
     1201 tttcgcacct tgatcgattg ctttttgcag tattattgta tcatcttgtt gagtgtcgtt
     1261 tgttttaaag ttcaaagcat tgataatccc tttttttgtc cgcaatacgt ttctccctat
     1321 ctttcattga tttttttcaa ttcttcacca ataaatacag tttttgaacg attcagtggg
     1381 taacctacta gcaaaatgat tgcggataca aataagcaga atgctggtaa caagtttgcc
     1441 atcgtataaa tcctttcttg tactaaaact gattggatgg ctccacctgt tgtagacgat
     1501 tggtaaccaa tgagtgccag cataaatccg cctaccgctc ctgcacatgc ttgtcctact
     1561 tttctcgcaa aagaatttac tccatatact gttccatctt ctcttaagcc tgtcttatat
     1621 tgatgataat caatcacgtc agtaatgaat gcccagacca tcaaattgaa taaaccagat
     1681 cctaaagtag cgataaataa aagaatcaga tatacccatg catttgttat atgaaggaaa
     1741 tacatcagca agtacataca tgaagaagca agtaacgcaa atacgcttgc ttccttttta
     1801 ccaaataatt ttgtcagctt tgtagcaaat ggtgctaaaa ctactactgt tccgtaagta
     1861 aataaaagag caacagacat tgcttgttta ttatggaagt aatcattgaa taaataagtc
     1921 atattcgtcc cagctaaaat ctgattgatc acaatgaaaa tgtcaacaat gactaaaata
     1981 atcagtgctc gattagctaa caaacctttt accaactgag aagcagatac tttttcgttt
     2041 ttgacaactc ttactctttc agtagtcaat ttacaagtta acatatatgc gacaaatgct
     2101 attgcagcac atccgattga aatccaaaag aaatgattcc ctgaaacgac ttgttgtcca
     2161 ctcttgttat gggtatacat taaaatcgga atgaagaatc ctgtagttga tccgccaatc
     2221 gcagaaccaa tgcttcggaa cgtggacaac gaggtgcggt cattaggatt tgagctaatt
     2281 gctgctgcca ttgctccata cgggatattg attgttgaga gaaaaatccc ataaacaatg
     2341 tatgaaacaa aaatataagc aatcttcacc gtcattggaa gatgttggac gaatggcaaa
     2401 aacaaaatga cagtcactaa gcaaaatgga tacttcattt ttctgatcca aggaataaat
     2461 cggccagttt ctgttaactc tgagacatca cataatcttc caaccatcac atcagcaaac
     2521 gcatccaaaa ttctcgaaat caagaataac gtacctacga ttgcccctga aacgccaaga
     2581 acattcgtat aataaatcat caagaaacta ttgactaaac ctaaaataaa acagttacct
     2641 agatcaccga acatgtagcc tatcttatcg gaaatcccaa attccttttt ttcttttttt
     2701 actgtatttg ctgtaacagc attttgtgaa agcatttgaa ttcctcctac ctcttctagt
     2761 aaacgttttc aaaattataa ctcttttttt cttgttttta tattcacaat gttggctttt
     2821 tattgcatta tcttgacaga taaaaaacaa aaaataatct aaaaacattg atgcataaga
     2881 ttgataactt tttttacata acaatatata caaacaaaaa aattcactaa ctagtgaaaa
     2941 taagttagtg aacctcattt attaaagaat taaattacag atgatctttt ccatcatact
     3001 tgaaaaactc aaaatcagca taacttcctc tttttttatt tagatcatgc actgctatcc
     3061 cgataaaatt tccagtaaac cctcctgaga gtatacacat gtttttttgc ggacctactt
     3121 tcttccattc ttcagttgat acgtctttca aatgaaactg tgctgtctct ttctttacag
     3181 tgactttcaa aaaagtcttc ttttctgtga gtggaatcgt ccaatcttct atttgtgcta
     3241 ctccgttttc tgattctatc atacgtaggc attttccctt gtcctcattc caagtaacat
     3301 aacaataaat ataattggat tcatttaagt acagtaccag tccagccatc tgattaaaat
     3361 tattaggcat gtattcaaat gctgtctccg catcaaaagt aaaatccgtt tgccgaatag
     3421 ctaacaaatg atgttcgaag gtagattgaa gagattcacc ggaaatcaga cgtaaaaaac
     3481 ctggtctaca ttttgtatca caccaatcag catttggcaa aatgcgtctg gcattccagt
     3541 tcttctttag ctctccttca aatgtatcca taaactgatg atctttttct tgttgtacct
     3601 cttcatttgt ctcaatcatt atttcttcca tcggagttgt ctggctatct ttaagtctta
     3661 accaatcgtc ggacgtccaa tacacttctt gaatagcggt ttctcttccc aaaatagccg
     3721 caccgtttaa tggtctggta caaagatatg tcatgtacca tcttcctgac ggggtttgta
     3781 ctaagctccc atgtcctgta cattgcaatg tagaagcagg tttgtcacta gctgtcagca
     3841 taggaaaacc aggatcaagc tcgtaaggac cagtaatatt ctttgctcgg caaactgtaa
     3901 ctgaatgtcc tcttcctgta ccgccttctg ccgtcatcaa ataataatac ccgttacgaa
     3961 aatacaaatg tggtgcctcc gttttcgcta attctgtacc tgcaaatatt ttatagatgg
     4021 gaccatcaag cttcttcttc tctgcatcat attcttgtaa tacgataccg ctggatttat
     4081 tcggtgtgtc cagacgataa tcccatagtt cattgacaag ccattttttt cccgtttcaa
     4141 gatcatggaa caaagaagga tcaaagccgt tactatttaa ataaattggt tcggaccaag
     4201 gacctgtgat ctcagatgca gtgatcaaat aattgtggac atctttaaac ggacggcctg
     4261 cacttttgac atctgtgtaa acaagataaa acaatccatc agcataactg atctgtggcg
     4321 cccatatact tccatttgta ggatttcctt gcaaagatac ttgagtggtc aaaatatccg
     4381 ttgtatgtga ccagttgact aaatttttcg actcatatac tcggattcct ggaagccatt
     4441 caaaagagga gacaataatg taataccgct ctttcactct taatatatta ggatctggat
     4501 tgaatcctct taaaatagga tttttcactt tgatagtctg catgattcag gctccttttc
     4561 tcattcttac tcgatataaa ctgaatgccc cagctgctaa aaaagcaagt gaaataaaca
     4621 acacaacggc aaacagacca gtcatgtcct gtaataatcc aaatgtattt tctaaagaca
     4681 aagttgaatg atctacagca agcgactgat aacttttttt aaactctgac gcagacatat
     4741 tacttaaagt aactgctaac ccgccaaaga aaatcaaaga tagactgcca ccgaccattc
     4801 ctagcaatag ttcccatttt cttttcattt ctgaaccccc tgcttttcta aatgtatcca
     4861 ttcaggtctt ttgatcgatg tttcataatt gttattacgt tcagagaatt tgacagtctg
     4921 ttcatttttc ttgtcatccc aattatccca accagttggg tgaacatgcc cacctatctt
     4981 acacgcttca aataagacat ttgcatacgg acgccacggt cttcctaaat agacgtttct
     5041 agtatcaggt tctgcagtca aggtacagct ttcaaaacga taccctgttt tttgattttt
     5101 cggcgtagat gctgctgtga tgtatccagg atttttactg gctaagctat ggatatgaca
     5161 ctgatgaaaa acggcatctg ccccaccaaa tatgaaatcc accgttcctt caatccagca
     5221 atattcatag gtctgacgac aatactcagg attggcagaa gccggtgtac aaaaaggagt
     5281 accatctttt tgcaaatcag gcaacggacc cgtgcataag gtatcctgat aaccagatag
     5341 tcgacagttt tttaaagata cgcgatctcc tgaattgaat aaagcaacag cctgtcctac
     5401 cttttctcct ggacctgctg tattttcgat gtgaagattt tctaatacga cattcgatcc
     5461 ttctaagaaa aaagtagcac ttcggaaagt ccctaactgg ctaccgtcaa gatactcctg
     5521 ttttgcaaaa cgattaccgg agataactac tgttcctgct cccacaagtt taaaattgga
     5581 taatctgctt tctatatatt catcatatct tccttctttt actatcaaga cctttggatg
     5641 gctatccggc aggcctgcca aataatcgat tccttcttgg atagtttcaa aatccgtcgg
     5701 ggtatctata gcattctttc caattatcag tttgtctttc atcctcataa atgctccctt
     5761 atgaattgtt tccaaatatt tctcatttct tgtgtttcca tatgaccgcc tgttaaagaa
     5821 aaagaagaaa agttttctgg actacccatt gattgatact ttttgtccag cccttttctc
     5881 agcttcagta cgccttcgtc caagcacatc gtatcgtttt ttccaactaa gctaagacga
     5941 gggcgcggag cgattttttc ttggattgct aacgttgtat aagctgtcaa tagatttggc
     6001 acatagtaat aaaacccatg atgatctagg cggcgatgta gcaaaagtgt ttcgatgtcc
     6061 acctgtgccg ctagatcaat acaaactttg acacgagaat ccaatgctgt cacccaccag
     6121 cttaataagc ctcccataga aagaccaatc gttgcgagcc gttgagtatc tgtgtcttcc
     6181 cttgtctcca aataatcgat caagctgatc acatcataaa tacgcattcc ccataacgtt
     6241 tttcctgtta ataaaaattg tttgaacaac tcgctttcag aaatacctcc gcgttcttca
     6301 aatccccaag catcaatcga ccaaacagca tatcccattc ctgtcagtac ttctgcaaaa
     6361 gatggagaag ctaagtaagg agcaccatgg atcaattcac ttttcccgac tgaaaaatct
     6421 ccgccgtgag aatgcaggta aaggaccgtc ggaaacggtc cttctttttc tggataagca
     6481 tagagggctg gtactgtttc caattcattt aagtacaatt gatagtattc aagcataaaa
     6541 ccgttttgtt tttctcggta taatcgtttt ccactagggg tatgtctgct agatggagca
     6601 cctaaaaaat cattcaactg cataagcaaa gtttttacga acaaccagtt catccatatt
     6661 ttctgattga tttgattgga atttctcaat actgatttct ttagtatttt ctaatagaaa
     6721 tgctggtccc tcgtgatttt caatcgacat ctggttaaag gaaaccttac ccgcaaagcc
     6781 aatataaaat ccttgtctgc tcatttcctc tattcctgtc atcatagcag gaacaccggg
     6841 aactgcatct tgggccatcg agacatcgat attgtcaaat gtgatttctt gtatgaattg
     6901 ttccgctaat ccataaataa aacctgcaga agcatggaca ttccttgccg tgatattcga
     6961 aaaatggatc cttctgaatg ctgatgtccg ttcatctatc ggataagcct ttttttccca
     7021 tacatacggt tctttccctc gcggaccaca gaaataataa agattcagta taaatggaca
     7081 catcacatta tccatgacta tattggatac tcgaatatct tcaacgatcc cgcctcgacc
     7141 tcgtctagat ttcaatcgta ttcctcgatc agtttcttgg aagatacaat ttgaaatggt
     7201 aatattacga atactaccac tcatttcact tcctaatacg acaccgccat gtccatggac
     7261 catcgtgcaa tttgtgatcg tgatattttc gcaagcgatc ctttcgtacg tatcttcagt
     7321 ccctgccttg attgcaatac agtcatctcc cacgtcaata tggcaattac taatacgcac
     7381 atttttgcat gattctggat caattccgtc tgtatttggt gaatccgctg ggttcaagat
     7441 cgtcaagtta tcaaacgttg cattactgca aagaatcggg ttgatcgtcc aacttggtga
     7501 ctggatcaat ttaatatctt tcacagtaat tcgatgacag ttatggaaac tcatcaattt
     7561 aggacgagga taagcgagat tatctggttc atttctaaaa gtatgccacc atttttttcc
     7621 atttccatct aatgtaccga aaccagtgac agagatgttc tctacatttt gagcatagat
     7681 acaggaagca taaacttttc gatggactcc ttcccaacgt gaatgaacaa caggataatc
     7741 ttttggatcg tcagagaatt tcagaaccgc tcctgcgctt aaatgaagtt cgacattgct
     7801 ttttaaaaat aaagctcctg ttagaaattc acctgctgga acaactacgg tcccgcctcc
     7861 gtcacttgct gcagcatcaa tggcttgttg tattgcttca gtattcaatt catcaattga
     7921 tgcgccaaat gtcaaaatat catacatatt tataatgcct ccacttcaat caatgcttgc
     7981 aagaatgcac caacgccttt ttgatcatta caaataattg gttcactgat gtagtaagca
     8041 tatgaaccat ctcgctgatc tgctccgcct aaccctgcta cttgacagtt tttattcaag
     8101 ttcagccagc cttcttttgt ttccagaaca aattcatcaa gtaatccttg atgtgatttc
     8161 ttcacttgtg gaagccattc ttctttgttc agcagtccca aacgtattcc tttagccatt
     8221 gcgtaaacaa acatactact gccggaagct tccagataat ttcctttccg ttttgtttct
     8281 gttgttactt gataccatgt cccgctgttt tcttcttgat atggaagtaa tgcagccagt
     8341 gtcttttcta gaatttcttt cagcaaagaa caatctgttt cttcatgaag taattcatag
     8401 gtatctgctg cagccatcaa ataccagcca atcgatcggc tccaaaaatg atgggataga
     8461 cctgtttttg gatcagccca tggttggacc ttcttttcat cccatgcatg aatcagcaaa
     8521 tttgtttttg gatcgaatag atgtttataa ctgatttcaa actgtctcaa aacatcttgg
     8581 taatcttttt cttcactaaa attctggata aattcagcat aaaaaggaga acccatatat
     8641 aagccatcca accaaatttg ataaggataa atttctttat gccaaaaagc tccttcggat
     8701 gtgcgtggat ggtttgccaa ctgctgggaa aggagttctg ctgctttttt atatttttct
     8761 tcgcctgttt ctttgtacaa taaaaagaaa agctttcctg tatttacatg gtcgatattg
     8821 tattcttcaa cgctgtagcc acggatcgtt ccatcttctt gcacgaaata gtcgatattg
     8881 tcttttatga aatcaaaata aattttttct tgggttcgtt cccaaagtaa ttggaaccct
     8941 ttaagtacga cgccatagtc ataagaccat tttcctttat accacttttc ttcatataag
     9001 cgaggtgttc tttccatgat actttttgct aatttctttg accagttttc cattatttgc
     9061 tctcctttaa aaattaaaat aggatttggg acaaatacca agaagtattt gtcccaatcg
     9121 tctatattac tgtgccgctg ctttgtatgc ttcctcgtac tcttgtgtga ttcgatcgcc
     9181 tccttgtgct ttccagtctt ctacagcttt tttgaatcca tcttcgtcaa gctgtcccat
     9241 gatgtattgc accgtagcat caatgacgat tttttcaatt tctgatccta cttcattgtt
     9301 cgtcggtgaa tccaatggca cagaaggatc cattacagca aattcttcat tttctttgat
     9361 caattgatta gagaattctt tttcagggtc tgtatctttg aaagagaacg tcacttcact
     9421 aggacgagag cttgataacg gttgcacctc ttgttgccaa agatccatat cgataaattc
     9481 gatagaacca tcatcattca attcataatg cgttccttcg ataccgccag tcatcaattt
     9541 atacacatct tcttctaaaa gatcatcaac aaattgaagt aatttcttca attgtgcttc
     9601 gtcttttact tgagatttgg gaaatgctag caagccgcca ataccatttc cttctgacca
     9661 aacatgataa ccgccgcctt caccatctga gatcttgttt acaggcacta attccatatc
     9721 atcttgaata ccttgaccca tcgtcttcag attcttgata tccaccattc cagtgtagat
     9781 tccagttctt ccttgtgcaa attgttcttg ctgatcagtc ttttgggtaa ctgcaaagtc
     9841 ttgtgccaaa tagccattct caaatagatc tcttgaaaaa ttcaaagctt tcatatactc
     9901 ttctgtatca aattctggag tgaatttgcc attatcgtct acagcccagc catttggtgc
     9961 tccaaaataa gaagtgaaaa gtttaaagct ggtataacga atatcactag gcgcacgatc
    10021 gccaaaacca actgtgtcgt ccacaccatt tccatcagga tcgttttctg taaattgttt
    10081 agctacttca taaagctcat ccatggtagt tggaacatct agtcctagat tatccaacca
    10141 gtcttttcga atgactaagc cagcacgagc gtagtctttt tgtatcggta cgccgtacaa
    10201 ggcaccgtcg atactcgcag cttcgattct atcttcagaa atttgactta aatttttata
    10261 gtcttttaaa tattctgata catcccagaa tacgcctgat tttaacgcat tgcggacaga
    10321 agagtttttc aacatcgcaa tttgaagtgt aacgatatct cctagttcat cagaagccaa
    10381 tgcagttgtt aatctttctt cttttgaagc atcaggaatc cagttgaatg tcagttccgt
    10441 atcggttttt tcttcgatca agttcacaat cgtgtctgta ggcggtgaag catgatgcaa
    10501 aatatttaac caagtgattt tagcttttcc atcttctgtc tctgaagaac tgttacttga
    10561 actgctgccg catgcggcaa gtgctagaat tcctgcaaaa ctaagaccta attttacgag
    10621 cgttgctttt ttcataaatt ttctccttca ctgttttaat aatatttttg ttttcttctg
    10681 ttttttctgt atatgagttc ttccattcca aatggcaaaa tatagaattt gctgtccacg
    10741 tgactgcgga aaagaagaga ctgccaccga taaaggttaa aacacctggc attgtttgga
    10801 tgatcatcag ataaatccct accatactta atatcaacac taatgagtaa tgaagatgtg
    10861 agaatccgaa taaaaatgcc attttcattt tgaaaaaata tcctttccaa tcataatgag
    10921 ccatgattgg gaaaatatag agtgctgaga gcacatatat gaacgcagca atatacaaag
    10981 cgatcctaag ataccaattg tgtatgaaag aaagatccac aagtaaaaca atcccgatgg
    11041 cgccatatat ccagctcatc agcaaggttt cacgatagtt ttctttaaaa tatgtgaagt
    11101 acgttttcgt caaaggaaac gtttcttttg agcgcagcca ttttctaatc actgcaacca
    11161 tcgcatacga tgcaggaccg aaagtaaaaa ctcccaaacc tgcgatgact aaaccgcccc
    11221 acagaaaatt caaataaacc aactgtaaaa tacgatttag ccttccgttt attttatcta
    11281 atgactgcag catatctttc cctctctttc tatcttagta aacgccgtct tctcctagct
    11341 ttttggcaat cttattagct aaaattacca taaacaatcc tacgacccct ttaaacaatc
    11401 ccattgctgt actgaaactt aactgtccat tcttcaatcc agctgtataa atataggtgt
    11461 cgaaaatttc tgcaacgctt cgattcaatg aattcaataa caagtacata tgctcgaacc
    11521 ctaagtctaa tgtctgaccg atttttagaa tcagcaaggt aatgattact ggacgaattg
    11581 ccggcaatgt aacatgccat gttttgcgta agcgtcctgc tccatccatt tctgcagctt
    11641 catacaactg tgtatcgact gcagttatcg ctgaaaggta gatgatcgta gaccagccca
    11701 actctttcca gatcacttgt ccgatatata ctgtgcgtgt ccattcaggg gatgttagaa
    11761 aactgatttt ttcaaatccc attgttgcaa ggaattcatt caatacccct ccgtcaacat
    11821 tcaaaaatac ataagtaatc gaaacaatga ttacccaaga catgaagtga gggatgtaaa
    11881 tgatcgtttg aatcccgttt ttcaatcgtt tgttcgttac ttcatttaat agtaaagaca
    11941 aaatgattgg catcggaaaa aacacgaaga tatttaatcc gaacaagatc aacgtatttc
    12001 ttaataatgt aaagaatgtc ggttctgtaa acaaacgaat gaaatgtttg aaaccgaccc
    12061 aaggacttcc cattattcct aaaaatggtt gataatcttg aaaagcaata acgagtcccc
    12121 ccataggaag atacctaaaa atcaagaaat aaaggactcc cggcagaatc atcacatata
    12181 aaaatttatt cgttttgatt cctgcaatta ttttttttcg tcttctgagc ttttcttctt
    12241 ttttcgatag cgtcagttga aatggttctt gtttctttac actttccata agcccctcct
    12301 tagttttgtg aatgtttgct gcttatgact tcatgataga taactatctg aattacgtat
    12361 ataatcattt ttatcgtaag taaaaacgct tacatagcaa tgatttttgt gctatacaaa
    12421 acgataaagt gtattttttc acaaataaac tacgcatagc aaaccttcta tttttttcta
    12481 tctgacactt agcataggaa atgattatat acgtaatctt tcaccgatga taaattgaac
    12541 atatagaaac agaaacattt aatttatttt gaggaggagc ctatctatat gaaaaagaaa
    12601 gtctctatag gagaaagaag ttttacactt ttcaacaata cgtttctaat tttgctagct
    12661 ttgatctgta tcgttccttt tttaaatatc atcgctacat cttttgcttc tacacaagaa
    12721 gtcgtcgcga aaaagtttat cctttttcct actacctttt ctttagacgc gtatcgctac
    12781 attctttcta cgccgaccat ttttagagca cttgctgttt cgattggtgt tacaggtgtc
    12841 ggaacgatcg tcagtatgtg tgcaacttca ctgatggcct acggtctttc cagaaagtat
    12901 ttgtttggtc gaggtttcgt caactttctt gttgtatttt ctatgttgtt cagcgggggg
    12961 atgattccaa cgttcttagt tgttcgttct ctagggctgg tcaattctta ctgggccatg
    13021 atcctgcctg tggctgttaa tgcaatgaat atgatcatta tgagaaactt tttccaagcc
    13081 ttgccggata gtttggaaga atcagcaaaa atggatggtt gcacagattt tggtgtattt
    13141 ttcaaaatta tgctgccatt agctttaccg tctattgcta ctatctcgct tttttatgct
    13201 gttacttact ggaacactta tatgactgct attttgtata tcaacgattc atctaaatgg
    13261 ccgatacaga ttcttttacg ccaaatcgtc attgtatcaa gtgggatgca agcagaaagt
    13321 agtgccgttg atgtgattcc accagcacaa acagttaaaa tggctgttat cgttattgca
    13381 acagtgccta tgctgattgt ttatcctttt gttcagaaat attttgtcaa aggagcactc
    13441 gtaggttctg tcaaagggtg attatgtact gaacgcaaaa tggtagttgt gtcagaagtg
    13501 gtcagcttca agaaataaga aggcatttac gaaaattgct tcttaaattt ttgtgaaatt
    13561 tcggcttatt tttcgaagaa tctactcctg gaacaccgtg tatcagaggt tgtgaaagag
    13621 acgttcagcc ttgagcgatg ataaaaactc tcacaaacat tgcgacgagt aacccacagg
    13681 agcaataaga agcgattttg aaaaatagct tcccatattt cacaaaatta tgccttattg
    13741 tcgatagcct gtctcttgaa cgccatttat tcgtaaaaaa gaactgtgac aagacttttg
    13801 tcacagttct ttttgcgtgc ttagccttga aatttaacta tccataaata caaatactag
    13861 ggttattcta acttctttta ttgcttgaat cagtccacaa gaaaaacagc ctcttgatta
    13921 aacggcgttt atcttgttga ttctgcggca ataaaatcag ccagcagcaa aacatgaagt
    13981 gctgtttttt gctgtcctct tttattgctt gaatcagatc acaagaaaaa cagcctcttg
    14041 attcacaaat atttttttcg atattctccc ggcgtaatcg attccttctt tttaaagaaa
    14101 cgaataaaat tttgcggatt tcgatattgc aatctttctg caatctcttt tacggagagg
    14161 ttagtctctt tcaaccattt ttttgctacc tctaaacgat gattttgaac aaaatcacca
    14221 aatttttctc ctgtttcctt tttaaataca ttacttaaat agttagggtt ataatgcaac
    14281 ctatcagcga tcacatctaa agacagatca cgatcatatt ctgtgtagac gatatgcata
    14341 atattctccg acaaattttt aaattcatga tcagtgattg cttgagagca ttctgtgatt
    14401 ggcagaacta attgttctaa aacaagtttt tctagttctt tgggatgata attgttcagt
    14461 gcttttacat aaatttgttt catgttatca aagttttttg aatctactcc caataactgt
    14521 cctaattgaa cgagttcatt gatcaagcgg accgaaacta cttcccgact caaaggattt
    14581 ttattttgtg caaacaatgc atcaatcaat tcatgtacta aattcttcac ttcctgattt
    14641 ccagaacgaa tggcttcaaa caatcgattt tgttcttcta tcggatatct aataagtgtt
    14701 ttttcatgag cagcaggaac gatttcctga taaaaaataa ttgaacttgg accagtattt
    14761 acacgataat acagggcttc ttttgcccga tcaaccgatt gcttgctttc attcagcgtt
    14821 tcgaaccggt tactgatgcc tatactgata gttaaattca agtattcttt tacacgtttt
    14881 tgaatgagtc ggcaatactc catgactttt ttgcttgctt ctgcatcatc tttatccatt
    14941 cgataaatag tggcttgcat ttcttcattc aaaacgatcg gcagcattcg attttccttt
    15001 ggcacaattt ccgtgatcat gttgttgatc gctaaaagta gcaaatcacg ctccccaccg
    15061 tgactatctt ctaacagatc gatttgaacc aaagctgtat aatagcagtc attttgagaa
    15121 ctataaccaa attgctgcaa ccgctgatct agttctcgcc tttctactcg atttctaaat
    15181 aaactaagta caaatagcgt ttccagctgt ggcttttggg taactaagtg tgcacttaac
    15241 gcttcttttt ctcctatgat ccgactgact gattgagcga ccaaatctag ttctttccca
    15301 ctgaatcctt ttgatttaag attcagacgt tcctgtattt gagaaatcgg acgggcaaat
    15361 ctgtccgaaa ataagtagga aaaaagacca atgagtccta ccagtgttaa agacactgca
    15421 actagcccga ttctcagatt gcggatcatt gcgccaattg cactagagtc aacttctaat
    15481 ctgtaaatcc aattattata atctgatttc agtactatat acgttttatc tttttctctt
    15541 atcactttca gctgattgta atctagatcc acgtccgtta atacagttgg ttgctcttta
    15601 ttttttactg tctgagtagt aaaaaggttg ccaacttcgt tttcaataga aagtatgtct
    15661 tctccacttt caccaacaac ttgttctatt gtatgtttct ctatcgtagc aattcccaat
    15721 gcatattttt ctttttcaaa cattggcaaa gaaatcacca tttcgattcc tttgtttttc
    15781 ttttgccaaa ataaattatt tttgttattt atgtacttct gttgatactt ttcaacttcc
    15841 tcttcggtca gttgactcaa cgatccattg attactcccc atttaccagc caaactgact
    15901 aaatcataat tattatcatc cataacaatc gtttctataa aatttagttc tttcctgatt
    15961 tcagaataag tggagtaatc tgtatatgtc agcggatgct tgaatatgtc cgagaaattt
    16021 ttggtcgaac catacgtaga aaacgaatat tcaatcgttc taattttcca ttcaacgtta
    16081 gttttaactt gcctgagcaa attctttttg tctgttaaat aactcgattc gatcgattca
    16141 taggtattct ggtaaatgaa taaactaaat cctatgacaa gtacggctcc taataaaaac
    16201 atcggtaaaa atattttata aaatactgta cttttcttca ttttcttcct ctcctccttt
    16261 taaaataaaa ggctttcatt tttctgtaaa attgatactc atctaattaa atgagcattc
    16321 cccgctttca agaatagcat aattaaaaat aaaaatccaa gtaattttta acgtgtgcaa
    16381 tcttttcata tcaaagatct attgacttat cgtagttatc gtaatatgat gaatgcgtta
    16441 acaaaaagtg aaaaagtaag gaaaatgatg gaatggtaac gatcaaagat atcgctaaag
    16501 cagctggcgt ttctcatacg acagtttctc gtgctttgaa taacagcagt ttaataaaaa
    16561 atgaaacaaa acaaaaaata cgacaactag ctaaggaaat gaattattcg ccaaactata
    16621 gtgcaaaagg tttagtgaat caaaaaaaat atctaatcgg actatttttc tcaagtttgc
    16681 ataaaggaac ttcatcaaat ttcctagcag attccattgc aggcatccat tcagtattag
    16741 acacaaatta cagtttatct gttgaaagta tcgatgttgt tgatttaaca gaaatcaacc
    16801 tgcaacgcta cgatggactg atcgttatga gtcaaagtga tgatgatcag cctttcatcg
    16861 actatctcaa agaaaaagaa tttcctttcg ttgttgtcaa tcgtcacatc aatgatccag
    16921 cggttatcaa cgtcactgcc gatgatgcgg ccggtgttat gcaggcagta gattacggta
    16981 ttggatgcgg acatacgaag atcgcttata ttggcggaaa gaaaaatttt cgttcgacaa
    17041 aagaacggga aaaaggggta ttgaaaagtc taaaaaaagc gagcatccct gtcaatctag
    17101 cctatttttt aacaggagat tacagcttag aaagcggcct ccaaaacatg gaatacctat
    17161 tatcactttc tgacctgcct actctggttt tttgcggcaa cgacgacata gcgattggtg
    17221 cattgagagc tgtacattta gctggcctcc gtgttcccca gaacatttct ctgatcgggt
    17281 tcgatgattc tccagtcgtc tcctatctca atccgccgct taccaccgta cgtaagcctt
    17341 tgaaagaaat gtgtcaagaa ggaacaaagc ttttactaga tttgatcgaa ggcaggacga
    17401 tcgaacaatc taaaattgag ctctccacct tgttgatcct tcgtgagtcg atcaattact
    17461 tgtaaactac aagtaatttt atataaaaat gttaacgtgt gcataacaca gaaaggaagt
    17521 ttatatgaaa aaactgacaa aagaattagt gaaaaaaaac gaagcacctg taaaagttct
    17581 tcagtttggt gaaggcaatt ttatgcgtgg atttattgat tggcagattc agcagctgaa
    17641 taaccaacaa ttgtttaaag gtaatgttgc aatcattcaa cctttagaac aaggtcttgg
    17701 aaatatgatg aaccagcaag ataatttata tacggtcatt ttacaaggac tcagcaatgg
    17761 cgaaatcgtt gatacttccg agattatcac cgtagtagaa caaatgatca atccttatga
    17821 tgaatgggat gcttatctat ctctagcaga aaatgacgac ttggcctata tcgtctccaa
    17881 tacaacagag gcaggaatcc aattcgtgga gactgataat ttaacagata ctcccccaca
    17941 gagcttcccg ggcaaactga cagccttttt ataccgacgt ttcaaattaa acaaagctgg
    18001 ctttaccatc attccttgcg aactgattga cagaaacggc gaacgtctaa aagaaatcat
    18061 tttacgctac gcagaaaaat gggatttaga acctgctttt gttgagtggt tagaaaaaga
    18121 aaatgttttc tgttgtagtt tagttgatcg catcgtccca ggttatcctc gcgaagatgc
    18181 aggtgttttc aatcaacggc ataactatga agatcaattg atggtcaaag cagaaccatt
    18241 catgctttgg gtgatcgaag ggcctaaaga attggagaat caactaccat tcaaacaggc
    18301 tggcttgaat gtcatcatca cagatgatat gactccttat cgtgaacgta aggtgcattt
    18361 gcttaatgga ccgcatacag cgatggtccc tcttgctttg ttggcggaat tggaaactgt
    18421 agaagaagta atgaaagatc ctttattctc cgcatatgtt gatcagttat tcaatctaga
    18481 attgattcct atgctttctt tacctaaaga tgaattagcc atttatgctg accaaatcaa
    18541 agaacgtttc ttgaatcctt ttgcccatca caaattagaa gcgatttcat taaatagtgt
    18601 ttcaaagttc agcacccgtc tgcttccagt gttcaagaag tatatcgaag aacaaaatca
    18661 ggtaccaccg ttaatcactg tttcactagc tgcgctactt cttatgtatc gaggcgatca
    18721 agtaaagcca catgatgatg aaaagacgat aagcgaattt actgatgctt ggtctgataa
    18781 tggaacagct attcctcgat tattgcaaaa cgctgcgctt tggggagaag atttgtctca
    18841 aataccgaat gtgacagata cagttcaaga aatagctgca aagatcgatc aaaaaggtgt
    18901 ccggtcagta ttacaagaaa tgaaaggaac gatggtccat gcttgaaacc ctgcaaccaa
    18961 caatgaaatt aaatcgacga gacagtgtgg tcgtcgcatt aacccctatt ccaaaacaaa
    19021 cacaactgac gattgatcag caaacgatca ccactcttga agatatcccg caaggtcata
    19081 agattgcatt gggtgatttg aaagctggag acaacgtgat caaatatggc tatccaatcg
    19141 gtcatgtgac gacagacgta actgccggtc aatggctgca cactcataat gtaaaaacca
    19201 atctttctgg tgaattggat tatcgatatg aaaaagatgt ccatccaagt cattaccttt
    19261 ttgagaaccg aacatttcaa ggttacttgc gaaaaaatgg aaaagtaggg attcgtaatg
    19321 atttgttttt agtacctacc gttgggtgtg tgaatggcat tgctgaactg atcgtcaaac
    19381 agttcaagga aaaacatcca gatctgggac agtttgacca tattacgatt ttaaagcatc
    19441 cttatggctg ttcgcagctt ggaaaagatc atcaaaatac tcgagaaatt ttagcggacg
    19501 cagtaaatca tcccaatgcc ggaggagttt tagtatttgg attaggttgt gagaacaata
    19561 cagttcctga attcaaaaaa attttaggtg aatatgatga agaacgagtg aaatttctag
    19621 tggctcaaga tgtttacgat gagatcgaac aaggtgtggc tctattagaa gagcttttaa
    19681 cagcggcgga aaatgaccag cgcatatcag tcccgctctc taagttgaat gtcggtttga
    19741 aatgcggcgg ttctgatggt ctatcgggaa taactgctaa tcctttactt ggtgcatttt
    19801 ctgactactt gattgctcaa ggaggcagca cgatcttaac agaagtgcca gaaatgtttg
    19861 gcgcagaaca agtattgatg gcacgagcgg aaaatgaaga agtttttgac tcaatcgttg
    19921 acttgatcaa tgacttcaaa cagtatttct tgtcttacgg ggaaccagtt tacgaaaacc
    19981 catctcctgg taataaagca ggtgggatca ctacattaga agataagtct ttaggctgca
    20041 cgcaaaaatc aggaagctct cctgtcgttg atgtgctaca atatggcgaa aaaatcagaa
    20101 aaccagggct tagtttattg caagctcccg ggaatgatct tgtagctgcc tctgctcttg
    20161 cttcatccga ttgccagttg gtcttgttta ccacaggaag agggactccg tttggctcat
    20221 atgtcccaac ggtcaaggta tctacgaata caactctttt tgaacgcaaa ggtcattgga
    20281 tggattttaa tgcaggcgtc ttattagaac aaccaatgga agtaatctta gaatcgtttg
    20341 ttcaaaaaat catcgctgta gctagtggag aagaaacgaa aaatgaacaa aatgacgttc
    20401 gagaaattgc catttttaaa aacggagtaa cactttgatt ttcataaaac agtataaaaa
    20461 gaaagaagga agatttatgt ttttatctga tgatttttta ttaaaagacg attgggcgaa
    20521 aaagctttac cattcctacg caaaaaacat gcccattatt gattatcact gtcacttaga
    20581 tccaaaagaa atttacgaaa acaacaattt cagcaatctg acagaagctt ggttaggagg
    20641 cgatcattac aaatggcgct tgatgcgagc ttgcggcgtg cctgaagaaa aaatcacagg
    20701 aaatgcttct gattttgaaa aatttttagc ttggtgtcaa accgtaccaa aaattatcgg
    20761 aaatccgctt tacagttggt cacatcttga attgaagcgt ttcttcggta ttgacttgtt
    20821 gataaatgaa gaaaatgcag agaaaatttg gacacaagca aatgaaattt tggcagctcc
    20881 tgattttcgc cgtcgggaaa tcgcaaaaaa atcgaatgta gaagtgattt gtacaacaga
    20941 tgatcccgca gacgacttgc actatcatca gttgcttgct catgaagaac cagatttgaa
    21001 ggttctacct tcattccgtc cagataaagc actcaatatt gaaaaagaag gatttaatgc
    21061 atgggtcaca ctactggaag aagcagctga ccaaacgatc accacctatc aagaactgat
    21121 agaagtctta gggcagcgga tagaatactt ccatcaacat ggatgtcgtg tttccgacca
    21181 cgctttagac atcttacgct ataaagaagc ggatgaaaca gtattagaag agatttttca
    21241 aaaagcacga gctaacaaac tattatcttc agatgaaatc gatgcttatc ttacagaaac
    21301 actgatacat ttgacccact tctatcattc tcataattgg acgatgcagc tgcacatcca
    21361 tgcttatcgt aactgtaata cacaaatgtt ggaaaaatta gggcccgata ccggctatga
    21421 tggaatgaat gatctttctt tgactattcc tctgcaaaaa ttattgaacc gtgcagaaga
    21481 aacggatcag ttgccaaaga cgatccttta ttcattgaat ccgaatgact atcctgcgat
    21541 tttagctttg atgggctgtt tccaaaaaga acagaatgga aaactgcagt taggttctgg
    21601 atggtggtac aacgatactc gagctggtat gcgcgatcaa ttgacacaat ttgctgatgg
    21661 tagtgctttg gggaattttg tcggcatgct gacagattca agaagtttcc tgtcctacac
    21721 tagacatgaa tatttccgtc gtgttctttg tgaattttta ggcgaaatgg tcatgcgagg
    21781 agaagcacct gaagatgaac aacttttagg aagtctagtt caagcgatta gttatacaaa
    21841 tgctaaaaat tactttggct tttttgccta attgattaat agtaacagta acgaatcaaa
    21901 aacaggatag tgaaataacg gaatccgttt atccacgttt tagagaagaa gacggaccaa
//
LOCUS       DARDHA010000030        21909 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000030 DARDHA010000000
VERSION     DARDHA010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.030"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="SDA10_000947"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="SDA10_000947"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEG4239875.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="SDA10_000948"
     CDS             complement(1172..1867)
                     /locus_tag="SDA10_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEG4239876.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="SDA10_000949"
     CDS             complement(1880..2596)
                     /locus_tag="SDA10_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEG4239877.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="SDA10_000950"
     CDS             2926..3534
                     /locus_tag="SDA10_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEG4239878.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="SDA10_000951"
     CDS             3859..4182
                     /locus_tag="SDA10_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEG4239879.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="SDA10_000952"
     CDS             4364..5734
                     /locus_tag="SDA10_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEG4239880.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="SDA10_000953"
     CDS             complement(5864..7165)
                     /locus_tag="SDA10_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEG4239881.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="SDA10_000954"
     CDS             complement(7519..9477)
                     /locus_tag="SDA10_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEG4239882.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="SDA10_000955"
     CDS             complement(9654..9920)
                     /locus_tag="SDA10_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG4239883.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="SDA10_000956"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="SDA10_000956"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEG4239884.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="SDA10_000957"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="SDA10_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEG4239885.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="SDA10_000958"
     CDS             13264..13827
                     /locus_tag="SDA10_000958"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEG4239886.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="SDA10_000959"
     CDS             complement(14080..14601)
                     /locus_tag="SDA10_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEG4239887.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="SDA10_000960"
     CDS             complement(14604..15446)
                     /locus_tag="SDA10_000960"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEG4239888.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="SDA10_000961"
     CDS             complement(15439..16164)
                     /locus_tag="SDA10_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEG4239889.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="SDA10_000962"
     CDS             16340..16636
                     /locus_tag="SDA10_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239890.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="SDA10_000963"
     CDS             17204..18796
                     /locus_tag="SDA10_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEG4239891.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="SDA10_000964"
     CDS             18809..19549
                     /locus_tag="SDA10_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG4239892.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="SDA10_000965"
     CDS             19717..21066
                     /locus_tag="SDA10_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4239893.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="SDA10_000966"
     CDS             complement(21194..21784)
                     /locus_tag="SDA10_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEG4239894.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6292 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaa
//
LOCUS       DARDHA010000031        21764 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000031 DARDHA010000000
VERSION     DARDHA010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.031"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(206..1105)
                     /locus_tag="SDA10_000967"
     CDS             complement(206..1105)
                     /locus_tag="SDA10_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEG4239895.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1115..1471)
                     /locus_tag="SDA10_000968"
     CDS             complement(1115..1471)
                     /locus_tag="SDA10_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG4239896.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1826..2122
                     /locus_tag="SDA10_000969"
     CDS             1826..2122
                     /locus_tag="SDA10_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HEG4239897.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2645..3598)
                     /locus_tag="SDA10_000970"
     CDS             complement(2645..3598)
                     /locus_tag="SDA10_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG4239898.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3621..4376)
                     /locus_tag="SDA10_000971"
     CDS             complement(3621..4376)
                     /locus_tag="SDA10_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4239899.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4373..5332)
                     /locus_tag="SDA10_000972"
     CDS             complement(4373..5332)
                     /locus_tag="SDA10_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEG4239900.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5325..6275)
                     /locus_tag="SDA10_000973"
     CDS             complement(5325..6275)
                     /locus_tag="SDA10_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEG4239901.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6425..6877
                     /locus_tag="SDA10_000974"
     CDS             6425..6877
                     /locus_tag="SDA10_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEG4239902.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(6966..9233)
                     /gene="gshAB"
                     /locus_tag="SDA10_000975"
     CDS             complement(6966..9233)
                     /gene="gshAB"
                     /locus_tag="SDA10_000975"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEG4239903.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9420..10418)
                     /gene="ftsY"
                     /locus_tag="SDA10_000976"
     CDS             complement(9420..10418)
                     /gene="ftsY"
                     /locus_tag="SDA10_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEG4239904.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10429..11238)
                     /locus_tag="SDA10_000977"
     CDS             complement(10429..11238)
                     /locus_tag="SDA10_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG4239905.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11247..14828)
                     /gene="smc"
                     /locus_tag="SDA10_000978"
     CDS             complement(11247..14828)
                     /gene="smc"
                     /locus_tag="SDA10_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEG4239906.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14848..15534)
                     /locus_tag="SDA10_000979"
     CDS             complement(14848..15534)
                     /locus_tag="SDA10_000979"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEG4239907.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(15882..17663)
                     /locus_tag="SDA10_000980"
     CDS             complement(15882..17663)
                     /locus_tag="SDA10_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG4239908.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17689..18603)
                     /locus_tag="SDA10_000981"
     CDS             complement(17689..18603)
                     /locus_tag="SDA10_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4239909.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18630..19592)
                     /locus_tag="SDA10_000982"
     CDS             complement(18630..19592)
                     /locus_tag="SDA10_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4239910.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19592..20536)
                     /locus_tag="SDA10_000983"
     CDS             complement(19592..20536)
                     /locus_tag="SDA10_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4239911.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20537..21541)
                     /locus_tag="SDA10_000984"
     CDS             complement(20537..21541)
                     /locus_tag="SDA10_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4239912.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         6096 a         4637 c         3836 g         7195 t
ORIGIN      
        1 ttatattttt ttttgtgatt atcgttcaaa aaaagaatac acgcttgcat ctagtactct
       61 gcgagttaca aatcatccga tcaaataaac attcgctgat tattttccgc aaaaaaagca
      121 gcatatccac gataaatgga tctactgctc ttacttatcc ttatgtttta caaggtattt
      181 tcttttacac attactaatg ctgtctcaat acggaacgat tgtcatcgct tgttcaaatt
      241 cctcttgcaa atgttgtgtt tgataggcct gaaaagctgg gtgatgggca cgcaagagga
      301 cacgtaattt cataggatca tagctgtcta gcaactgttc tttcaagaca aaatctcggg
      361 tcagctgcca ttcataataa taaccaaaag gtctgcagct gtaaatcgtg tgaccgatgg
      421 atttatcttc aaagcgacga tgcgtatggc caaaaactac ttgtttgata tttgaataac
      481 ggtcaagtaa ttctccgaat gattctgatc ctaaaaatgc atttaatttg ttccaacgct
      541 cataaggagc agattgatac atgataaatg cccgtttagg aacgaaatga gtggataaaa
      601 tcacctcaag tttttttgct tccagctggt ctagcaaatt ttttgcagct gcaagcattt
      661 ctgcattgac ctcgagatct gttttcccac gctggatgaa ccgatcatac caatacaaac
      721 gcttcattcg tacgattgcc tcgtaatctt tcaattcgga aaactgatag tcgtaccagc
      781 cattcatccc taaaagaacg gtctgctcat ttaaagcaat ataccgttca tttaaaaaat
      841 gtgggtctgg aaaatggtcc atcatctgtt cattgggcac atctgccaac tcgtgattgc
      901 cgaaatgaaa tgtcgtagat aatcctttat tgacgaaaaa ttcgacaaca gctaggcaac
      961 ggtctacttt attagccgta tctccggcta gatgaagccg agtgatcttg tgcttttgca
     1021 aaacttgcca aagctgcatc agctctgatt cgccgaattt attgatgtcc acatgtaggt
     1081 cgctgatgat cgccaatttt cccattttat ccctctagtc ttttcgaaca acttgcaagt
     1141 tgccttttct caagccgctt ggtcctttta attgtaaact attataagtt ggttctaaag
     1201 caagtgtgga tggatcatcc atgtaaagta ttgcactgtc tttgattttg attggtccaa
     1261 ctggtgtatc agccactaca gaataaagac ttagatctgt ttgtgtatct tttttgacaa
     1321 tgatcaatcg aaatgccgta tccaattggc aactgacttg gctttctgca aatggaccgt
     1381 caccgttttc gatatctaat aaaagttgat cgtctgcttc taatttattc ttcaatactt
     1441 cttgtgcttc ttttgtcact tctaatttca tctgtatcac ctcatgtcat agaataatca
     1501 ctttattcat cagatgcaac taatttgtct ggttctgact gttctcaatt tattggaaag
     1561 taagtcattc gtttattatg atttctctta tcacaaaaga gacaactttc ccttttcttc
     1621 gttcagtttc ttgaacggaa aaagacccaa aaagcaacct gttatttgtt taaccttatt
     1681 cctttcttaa ataacaaaaa atactataaa aaataaaaaa cctttaacaa ctattggaaa
     1741 aagaattact ttttgttcac ggattattaa caactcttcg gtagagtaaa cttatcaaat
     1801 aaaagacttc tttggaggaa tcactatgaa ggaatatata aaaaacatct attttatcga
     1861 agaaacacaa aatatcgagg gttcttatat tgaagtaaaa acactctttg tcaatgaaga
     1921 taaaaccaaa gcattggata tttataaaaa attagcttca aaaaaaacga atagttttgg
     1981 attgatatta agcgagtata aaatcaaagc agaagagagt tatttctatc aattattaaa
     2041 acggtggagt aagcttcccg ccgattttta tcgaaaaatg cagatcatca actatcaacc
     2101 gcttgctgag acacatgcct aaatagaggt tgtgaaatca gtgctttgag ctaataccga
     2161 aggtcagctg attgaacatt gtttagaaga gattgtgaca actgcgattt gctttgagta
     2221 ataagaacgc tggacagaaa atagcttctc atattttcta gtctagtgga cttattgccg
     2281 agaagcagca gatggaacat cgtttaaaga agagatcggt acaaaataaa aatatctaaa
     2341 tttaagtttt atctaagttt cgctttgtac ctttgattca aatcaaagga ggcgatcttg
     2401 atgctgaaaa aatggatcac actatttttt gccactcccc attctagaat caacccgtat
     2461 gatgtagata atgaattcac gttgaaaaaa gatttctcaa ataaataaaa accatccgta
     2521 aaatcatcaa tacgatttta cggatggttt ttgataactt ctttgaggat aggctcttgc
     2581 tatttaagaa tgtaagaaac attattgtta catagtatga gagtttaatc ctctgttatt
     2641 tatgctattt aaatgcttga ttcacatctt cgatcatcag tttcattgat tctaaaccgc
     2701 ccccgcttaa ataccagaca tctggttcaa gggagatgat ctgctgattt ttaccagcat
     2761 tcgtttgtgc taccagttca ttggcagaaa tatcatcttt gctgtcgtct ccaccaatag
     2821 cttttgttct gtctactaca aataagatat ccggattttt ttccagcaca tattcataag
     2881 aaacactttg accgtgagtg gatgcttcga tttgatcatc tgcttgttca aagccaaata
     2941 aatcatggat gaaaccgaat ctagaaccag atccgtaggc tgataattgc ccttcattca
     3001 caagtgtgac tagtgctttt ttgtcagtag cttctgcttt ctttttcgtc tgatcgattg
     3061 cactagataa ttcctctaat tgagttttag cttcttcttg cttaccaaag atctccccta
     3121 aagcagtgac attttgctgg aaagattccc atggattttt cgcatctaaa gaaaggaaaa
     3181 tggtcggagc aatttgactt agatcttttt gaaaatctct ttgtcggcca gaaatgatga
     3241 ttagatctgg ctgaagctga ttaatttttt ctaagtctgg ttcttttatt ccacctgctg
     3301 attctacttt tttataggat gaaagatact caggaaggct gcttgtagct gctccaacta
     3361 ccttatctcc aacaccaaga gcatccatcg tatctaaaga accattgtcg aatacgacga
     3421 ctttttctgg attatttggc acctcgattt ggtctccatt actatctgtt atcgtcaatg
     3481 tagtttcagt ttgtgtgctg cttttttttg tgtctgcctg actggtgttg ttgctatttc
     3541 cacatgctgc taaaccaaat gatgctagaa tcaagatacc tactactaat tttttcatct
     3601 cttctctcct aatgttagtt ttagaaataa agacagaatc tgcggccttc aatctcacaa
     3661 atccgtatgt ccatttcgta taattcatcc aatacatctt tttttatcat ttcttgggtc
     3721 gttccttgcg taaataactg tccatctttc attgcaacaa tttcgtctgc ataacttgca
     3781 gcaaagttga tatcatgaag aacaataaca attgttttcc cgatttcttg tactaaattt
     3841 tttaaaatct gcatgatttg aaccgcaaaa ttcatatcta gattgttcaa aggttcatcc
     3901 agtaaaatat aatccgtgtc ttgcgctagc accattgcaa tatacgcacg ttggagttga
     3961 ccgcctgaaa gagtgtcaat cgattgatcc gctatttctt ctaacccaag gtgttccagt
     4021 gattcctgaa cgatttcctc gtcttttgtc gtcaattttc ctttcgtata aggaaatcgt
     4081 ccaaattcta ctaattcttt cacggtgatt tggacattta tttcattcga ttgtcgtagg
     4141 atcgataatt ttttggctaa ttcattggat ttccaagcct ttacttcgtt atggtctaaa
     4201 tagatttcac ctgtatcttt tggtatcaaa cgactgatca tcgacaacac cgttgacttt
     4261 ccagcaccat ttggaccgat aaaagcagtc aatttctttt caggaatcgt taactgtaca
     4321 tctttaacca ctgattttcc atggtacagt ttattgacct tttgtaaatc aatcatagga
     4381 tactcttcct ttcgcgaatc aattttgtga caaaataaat tcctccgaca aattcaacga
     4441 cgacactcac tgttgtattc cagccgaaaa tctgctctac tgcgagctgt cctcctgtca
     4501 gaaacagcat agctaccaga aagcttccga taaatagctg accatgacga taagtagaaa
     4561 agacacgata actgatattt gcgacaataa accctaaaaa agtaactgga cccacaagag
     4621 ccgttgatgt ccctgtcaaa agactgacac aacataaaat caaaaattca aaacgatctg
     4681 tgtctatccc gaggtttact gcttgttcat ttccgagatg gaaaacgtct agcacatgtc
     4741 gatatcgaaa taacaggtac cagcagccga taatgatcaa agaagcaggc aaaagatact
     4801 gactctggat attgccaaag cttgcaaata atctgccttg aagcaaatca tattcattcg
     4861 gatccatgat gacttgtaaa aaggtactga cactaccaaa aagcgtacct aaaatcatcc
     4921 ctgacatcag taataaaaaa agattcgctc tcattttttt caaaagaaag aaaaataaca
     4981 atagactcag cccgaccatc agcccaacgt tcaataagaa aaaaactgta cctgtctgct
     5041 gaagcatcgt cactcctccg ataaagaaga aaaggaccgt ttggacaagg acatataacg
     5101 actcaagtcc caatactgaa ggagtcaaaa actggttatg cgtaattgtc tgaaagctga
     5161 ttgtggcact gctgacagct agcgagacaa ggataaatgc aaaaattttc ttactcctca
     5221 atgcacatgc aaaatgccag tttccatatg tatggatcgt caagtacatt cctgtaacaa
     5281 ataaaacaag aacaagcata ccaataaaaa tagatggctt ttttttaagc atatcgtcgt
     5341 ccccttatca gaagataaag gaagatagcc ccaccgatga ttccaacgat cacactgact
     5401 ggtatttcat aaggaaaaat caacacgcga gcgacaagat cgcaaaatac taagaacaaa
     5461 ctacctgcta aagcagttac gccaattgtt ttgtgcactt gatctccata tattctagag
     5521 acaagattcg gaatgatcac tccgataaaa ggaatgcttc ctaccatgat caacatgact
     5581 gcactagcca atgatactaa gccaatgcct aaaaactgca tcttctggta atctattccc
     5641 agatttactg caaacgattc gcccagacca atgatcgtaa atcggtaagc aaatagccaa
     5701 accaagagca acaatggaac aacagcatac aataactcat aatttcctcg tatcacagtc
     5761 gcaaaattcc cttgaagcca tgaagtcata ttttgtacca actggaattg ataagcaaaa
     5821 aaagtagcaa ctgaaccaat gatattccct aacatgactc caatcaacgg aagcatcaat
     5881 tgatttttct ggaggaacaa tcgcgtaagg gataaaaaaa gcaatgttcc tgcaaaagca
     5941 aagcaaaatg ctgtcaagga tcggacaatc aaggatgcac caggtaggaa aatcatagcc
     6001 actaaaatac ctaaacgcgc actatccatc gtgcccgcag tcgttggtga aacaaattta
     6061 ttttgggtca aatgttgcat gatcaaccca caaatactac tggtcgctcc cacaaggatc
     6121 aaacaaatcg ttcgtggcaa acgagtcact agcaagattt gtaagctttg cccatctaac
     6181 tggagcagtt ctgacaattg tacattcgat actccgatga acaatgaaat actacttaaa
     6241 ataactaata atgctactag tcgtccgtag cgcatccaat ccacctttct aattgagaat
     6301 gattttcatt cttattttaa gtgaatcgtt gaacaaaagc aaccgctttt tcatatgttt
     6361 taggtattat tgggaatatg ctataatcgt aagaaagaca aagagaagaa ggagggtatt
     6421 ttttatggca caagatgatg taaaaagcca tttggataag ggactttatg gtacaccttt
     6481 agtgaaccct gaagaacagc ataaatattt agggacattc agggaacgct gctatcttag
     6541 tatgactgtt gctgaaatga tccaaccaaa aaacaaagaa aactttttaa aagaactcaa
     6601 aaaacatcca gatgcttcca tactcttgaa tggggctcta cccctttctg tccaaaacca
     6661 atatatccag ctagcaacga aaaacaatag ccgtttcact gtagtcaacg attacgtcac
     6721 tgcaaatcct gaggaattcg gattgctctt aacagcaaaa gaagcagtaa atgaacctgt
     6781 gattgatatc gaaaaaaaat acccagaaga aaactcttcg ccagctgaaa aagaaaaacc
     6841 aaaaaagaaa aaaagcttct gggatcgttt attttaaaac gaaaaaacac ataaattttt
     6901 acatgcaaaa aaagcttttg atcagattga catcaaatag gacaatctgt caaaagcttt
     6961 ttcttttaaa atacttcagg atataacaat ttcaatacat tcatcgtcaa tcttctgcct
     7021 tttcctgcaa aaggataaac atgcatatgc atggctggat tgaaatttgc ttcgatgatc
     7081 ccatatgctt ttttatcagt tgtaggatcg atttctttat ctggtataat caaatcgata
     7141 ccactaatct tagctcctag tgcttctacc gcagaaacgg cgattttttt gtaagcttca
     7201 gaaaattcat cagtcatatc gatcgaatca ccacctgtac tgatatttga gttctccctt
     7261 agataaacaa tttgattggc ttggggaact gattcgatcg tcaacccttg ttctttcaac
     7321 atcaattgtt ctaattcacc taattggatc aattctaaag gtgcacgatg attcgttcca
     7381 cgcaaagggt cactattttt ttcttcaaca agttctttca ctgttcggat actgtcacca
     7441 ataacattag cagggactcg caacatgatg gcttggactt ctccgtcgat cacaaagaaa
     7501 cgatattccg ttcccggcat aaattcttct actaaaaccg aactgtcttc acgaaaagca
     7561 atggctaaac ctgcttgata atcttccaga ctagctcctt ccttgaaaat cgtgatacct
     7621 aacccgtaat tggttgattt aggtttgatc acaaatgcct gctcggcaaa tctcggataa
     7681 gcttttaccg cttcttccat ggatgaaaat tctgcgcctc ctggtacccg gaacccagct
     7741 tcttttaaga cttttttcgt tactgttttg ttttccatga taagtgggac gatatagctg
     7801 tccttacttg tcatgtttgc atttttcaca tattctacat ggtcttggaa ttgcagacgc
     7861 aaaaattgat ccgactcatc taatactttg acttggacac ctttttggat ggcatcgaac
     7921 ataaatatct gagtagaaag ttccatttcg cgaaaaccgg ccaactgata gggacgttca
     7981 tggctttctt tataatattg atgtcccaat gccacagcaa cttcttgctg actcgatagc
     8041 tgtgcttctt gatagagttt cgctgctaac gtttgttcag gatgatccat ttgagtcaat
     8101 gcttcctcta ctaaataaag agaatccaac caatccaatt caaccagcat ttgcttcatt
     8161 ccttcaagaa tttctctacc ttcttgatga aattctgttt gatctgatgg ttgttctagt
     8221 gctactttgt tatttcgagt cgtaccttct gctacccatt ggtcacagtc ttctttttct
     8281 tctgtccata acatgtacat caggaataat tgtaagaacc gaacttgctc tggattgata
     8341 cccaatcttg cataaggatt caaatcgata ttccgcaatt caatataacg aacacctgtt
     8401 gttgctaaat ctgcgacttt ctttccgcct cggaaacgaa gcggtgtata aaattctttc
     8461 tcctccgaaa gtttcccttc ttcgatcatt cgctcgatat ctgctaaata ctgtttcata
     8521 gaagcatagg aaactttcac atttggatga ttcgtatagc ccagcgcact gttgcgaata
     8581 ctgcgaacag gttcttgcgg atgtgattca tctaaaaagt agttcttctc actcattggt
     8641 gaagcaccga ataaataagt gatcatccag cgatagcgca taaagtttct cgctgttttc
     8701 aaataaagtt ctgttttgaa ttcggaaaaa tcttgaaatt cttcctgatg gctgaacaat
     8761 gttcttagca actcatcgcc aaattcaaaa ttgaaatgaa taccgcttac catctgtttt
     8821 cgcttcccgt attcttttgc taaataacgt cgatacaaga catcttcgaa attttttaat
     8881 tttgcaataa tgatctcttc gtctttttcc ggtaaggcag gtggcatact taatggccag
     8941 atcatctctt ctttcggtat agaacgagca gtcacatcat aaacagcagc gagatactga
     9001 aataattcgg gaatagaatc ggtaacaggg gtgatcatct ctgtttgtgt ttcgctaaaa
     9061 tctgtttgga tataggggtg ataggatcga tcgccaaaga ttgctggatg atcggttttt
     9121 gctagatttc ctgcaagatc aacccgctgt ccttcccgtt ctataccaaa tcgagcttgg
     9181 tcgataaaag ggcgcgcatt gacatgcaat aataattgct taaaattcat cattaagtga
     9241 cctccaattc atatgaatct aattataatc aaaaagtcta agaaaaagaa cgaaattgtt
     9301 tgagaaccgc ttacaaataa gtataaaaat gaaggcatca aaaacacagc tatgttaaaa
     9361 acaatcattc aaacaaggag ctgcaacttt tgtcacagct cctgcttttt agacatgggt
     9421 tattcttctt ttagtaatcc tttgaacaat ccaacgacaa aatcatttgg atcaaacggc
     9481 tccaagtcat cgattccttc accaagaccg accaatttca ctggtaaatg caattcatta
     9541 cggatagcaa gtacgatacc gccctttgct gtaccatcta attttgtcaa aacaagaccg
     9601 gtcacatctg ttgtttcttt gaattgtttt gcttgaacca tcgcgttttg accagtggtt
     9661 gcatctaata caagaagtgt ttcttggggt gcttctggta attcccgctg gattactcgt
     9721 tttatttttt ctaattcgtt catcaagttc actttatttt gcagtcggcc tgcagtatca
     9781 actagtaaaa catctgcctg ctctgtcttt gctctttcca aagcatcgaa tacgacagct
     9841 gctggatcgc ctcctgcatt gccacgaaca acttcaacac cagcgcgttc gccccagaca
     9901 actaactggt cgattgctcc agcgcggaag gtatctgctg ctgccatcaa tactttttta
     9961 ccttcgtttt tgaactgatg ggcaagttta ccaatacttg ttgttttccc tacaccatta
    10021 acaccgacaa acaaaataac tgttagtccg tcttcttgga tatttagttc atttacttca
    10081 ttgattcctt ccgcttcata gagatccaca agtttctcga tgatcgcatt ttggacttct
    10141 gccgattttt tcgcatttct gagtttcact tcttgacgta atgcttcggt aattcgtatc
    10201 gctgtttcaa aacctacatc tgcaccgata agtgtttctt ctaattcttc aaaaaagtct
    10261 tcatcgactg ttcgaaaatt ggcaaacaac tcattcatcc gttcaccaaa tgttttacgc
    10321 gtcttttcta aacccttgtc gtacttctcc tgtacttcaa cttcttctgt tttctcttcg
    10381 ccagtaaaag cacgcttgat tttatcaaaa aatcccattt gactccccct actctaatac
    10441 aaatttttgg atcactttgg ctacgccatt ttcttcattg ctggctgtca cgaaatctgc
    10501 tgcttctttg acgaaaggca cagcattctc catcgctacg cctagtccag catattggat
    10561 catcggtaaa tcattttcct catcaccgat cgccatgatt tcttcttgtt tcaaaccaag
    10621 atcttttgcc aataaagaaa taccataagc ttttgtaaca ccttttggca taaattccaa
    10681 aagcatactt cttgttttga tgatctcata catttcatgg aattctgctg gtatttcttt
    10741 gattttagga tctaattctt cttggtcata agcgattact gccttattaa gcacaaattc
    10801 atctgtaata tcgcttatat ccgctggttg gaattgcagc agcttattca acacattgta
    10861 caaggattgc ttccttgggg cactaggcag ctgtaaaacc acattgtcag acaaaacatc
    10921 tagtggcaga tccaattgct gagccagttt gtataagcgt tggatatctg atacttccat
    10981 cactttcttt tccatgattt caccagtatc atttttttgt accaaaccgc cgttgaaagt
    11041 gatgctgtag tcaccttcat ctaccagtcc taattcttgc agataatgcg ccatcgcagc
    11101 taaggggcgt cctgtacata gaacgatctt gaccccttgc tgttttgctt gtgctaatgc
    11161 ttgtttattc tcatctgaaa tcagcttttg ttcatttaat aatgtaccgt ccaaatcgat
    11221 tgctactaat ttgatcattt tcgttcctac actttcgttt cgttgatttt cgcctcttca
    11281 ttgatatctt ccatccgtac agaaacgatt tttgatacac cggattcctg catcgtcact
    11341 ccgtacagta catctgctgc gaccatcgtt cctttgcggt gtgtgactac gataaattgt
    11401 gtgtcatctt ggaaatcgct caagtaacga ccaaaacgtt tcacgttggc ttcatctaac
    11461 gctgcttcta cctcatctaa gatacaaaac ggaacaggac agacacggat aatggaaaag
    11521 agcaacgcga tggctgtaag tgctcgttct cctccagaaa gcaaacttag actttgcagt
    11581 tttttgccag gaggttgtac ttcaatctca atacctgttt tcagcaaatc agttggttcc
    11641 gtcagtacta attcagcttt accgccgcca aacatatttg gaaagacaat tttgaattct
    11701 tgacggatag cttcaaatac ttctttgaat cttgtccgca cttcatcatc catctcatcc
    11761 atcgtttcaa acaattggtt tttcgctgct aacagatcat ctcgctgtgt tgctaagaat
    11821 gtatgtcttt cactgacttg ttcgtattgt tcgattgaat ttaagtttac tggtcccagt
    11881 ttttcaatct gttcttttaa ggatgaaacc tttgttctac tgtcttcgat atcggttgtt
    11941 tcttgatagt cttgggaagc tttctcaaat gtcagctgat attctgactg cagataggtc
    12001 aaatgattat ctaaaatcat ttcagctcgg tctttttgga cttctaattt ggattgttcc
    12061 gacagtcgtg ctttttgctc acgattacgc tctgccaaga ctgcttcaag cttatcgatc
    12121 tccttttgcc ttctatctcg ctgctctttg gcttttgcca actcggcttt taatgtttct
    12181 cgctgagcag ataattcgtt aatttgtttt tccaaacttt cttctgtgac ttcatgatca
    12241 gaaaagtctg ctgtcaatgt cgccaactgc ttttctatcg cttcttgacg ttcggttgcc
    12301 tctgcttttt ggactcgggc accgcggagc tggatctgca gatgattaaa ctgctctttc
    12361 aagacagcaa gatctgcttg ttcttgtgct ttttgacttt gtacttgtgc tcttctggct
    12421 tccatctggt cgctttcttg acttaacgat ttgatttctt cgtcgatttt ttgtcgttgc
    12481 gattcaagat ctgtttgttt gtctttcaat tcgttcgttt gcttttcgta atcttcgata
    12541 aactgctgca gttcacgtgt ctcaaaagtt gagatttgct tttctttttc aaaacgttca
    12601 agttcattta tgatgttttg tagctgattt gtcgcttctt gttcttcaaa acgtaattgt
    12661 tcgcctcgtg tacgcaaaac ttcttgttct tcggctaaac gagctgtttc ttgttgcagc
    12721 tcctgaactt ttttttcttg tgcttggagt tgtttatctg cttcttcaaa ttcactagtc
    12781 aactgtttca attcttgatt ttgtacaaac aaactgcctt ggtttcctcg tttattcgct
    12841 ccacctgtca tggatcctcc ggcattcatc acatcacctt ctaaggaaac cactcgatat
    12901 tgatagcgga tcgtttgcgc gattgcattg gcactagtta agtctttggc taaaaggatc
    12961 gttcctagca ggttttgaac cacagtttgg atttgatcgg gataactgac ttgttcactg
    13021 gcaatcccta taaacccttc tacagctgct gcttgtgtca atatatgagc cggtaattgt
    13081 cttggtttga ttgtcgtcag tggaaggaat gttgctcttc ctcctctttg ctgtttcaaa
    13141 tacgtgatgg cttgccgagc atctttttca ttttcaacga tgacatgctg ggctgcccca
    13201 cctaaagctg tttcgatagc taacgtgaaa tctgcaggaa catcgatcag ttcagccact
    13261 gctccgacaa tacccgaaag ctgctgttta tgctgcaaga ccaatcgaac cccttggtaa
    13321 aaaccaaaat agttttcttg gatttcttga aggcttttct tacgagcacg tagctgctgg
    13381 acttgattca ttaattgata cattttaggc tgttcttttg ctaatgcttc ttggaacttt
    13441 ttcccgtttt gttggatcat ttctaacttc tcttttgttt cagttaaaga tgattttagc
    13501 tgcgcctgct tttgtgtcaa ttcttctttt tgtaagatca atcggtctac tgatgcttct
    13561 acttccgatt gcttggctaa cgtctgcttg ctttttgctg tttcttgtat atattgacgt
    13621 tctagatact tcagttcgtt tcctacagct gcttgttctt gcatcaaatc aacatattga
    13681 tctcttaatt ctgccaataa ttctttcgtt gatttttggt atttttctaa ttcttgctgt
    13741 gtttttatga gatttgcttc agctttttgg atctctgttt ctttttctgc tgctgctttc
    13801 atcaagcttt cttgaagctt ttcaaaatgt ttaacctttt tttgtgcttc tgctaatgaa
    13861 gtttgatatt cttgcgagct tttttgtgta tgctttgtcc gctcctgcaa aacatctttt
    13921 tgtccttctg tctgcttcag tttttccgaa agatctaata atacttgctg atttttttcg
    13981 atcagacgat cggcttgcgc attttcttta cgctgcttcg ccaaaatgga ttcttgttct
    14041 tggatcgact cagatagttt gcctaattct agattgaatt tcgcaagctg tgcttgtttg
    14101 ttgtcccaat ctttttttgc tgtcttgatt tcagcaacca tcaaagagac atcggtttga
    14161 gtcagtgttt cttttagccg taaaaactcc tttgctgcct cactctgggc ggccaaagga
    14221 gttaactgtt cttccaattc gtggatgatg tcttggacgc ggctgaggtt gtcttctgtt
    14281 tcaaaaagct tctgttctgc tttctttttc cgctgcttgt atttcaagac accagctgct
    14341 tcttcaaaga tcccacgacg gtcttccggt ttactactga aaatcgcttc tacttttcct
    14401 tgagaaatga ttgagaacga ttcttttcct aatccagaat ctaagaacaa ttcttggata
    14461 tcttttaatc tgcagctttg tttattgatg aaaaactcgc tttccccagt ccgtcgataa
    14521 cggcgggtta cactgatctc attgtactcc aatggcagat aatgatcact attgtctaaa
    14581 atcactgtga cttctgcaat attcagctgc ttacgggtat cggaaccggc aaagatgata
    14641 tccggcattt ttccgcctcg cagactttta gcagattgtt ctcctaatac ccagcggata
    14701 gcctctgtaa tattgctttt tccgctgcca tttggtccaa ccactgctgt gacgctattt
    14761 tcaaaatcaa tgactgtctt gtctgcaaat gatttaaaac cggctatttc aattcgtttc
    14821 aaatacaaag ctttccctca cttactttta cagtgctgcc aatgcatttt ccgccgcagc
    14881 ttgttctgct aattttttcg atttcccttg tccagtgcca atcaattgat catctacata
    14941 aacttcgatc caaaatactc gttcatgagc tggaccttct tcattgatca atcggtattc
    15001 gattgataca tcacctgatt tttgcaagac ttcttgtagt tttgttttat gatccatctc
    15061 atgtgaaaaa gcacctgctt tgactttagg aaaaatcact ttttcgatga atgcatgggt
    15121 cgtgtcgaat ccctggtcaa gatataacgc gcctaaaaaa gcttcgaata ggtcgcaaag
    15181 caatgctggt cgcgtacgtc ctcctgaatt ctcttctccc ttgcccaaca aaacatattg
    15241 atcaaagtga cattctttgg caaatttcga caagctgtct tcgcgaacga tcgcagctcg
    15301 tgtttttgtc aattttcctt ctggcaaatg aggatataaa cggaataaat actgagaaac
    15361 catcagttct aaaacggcat ctcctaaaaa ttcaagtcgc tcattatccg ataattgcag
    15421 attgcgatgc tcattcacat aggatgaatg ggtaaaagct tgttcaagca aatttagatc
    15481 gtgaaaaacg atgttatagt tttcttttag ttcttttgtc agctggttgt ccattttcca
    15541 cattcctttt atggatattt tttcgatact tccttattat actggttttt tgtcaaattt
    15601 gaaatggttc ggtcaaaagg taagtaaagc tttaataatc ctagtgattg acgagaagtt
    15661 gtgaaaaatt cgtttagttt cgagtatgaa gaaaaaattt tgtgaaaatg gctttccata
    15721 tttttgcata aatttgactt aataccgaga aactggactt tgaacaccgt ttattggaga
    15781 agtcatgaaa tcagcgcctt gacctgaata ttagagaaat aaacagaaaa atatcaaaaa
    15841 accgaaaaag gacatcgccc tttttcggtc atttttagtg attatgaacg tgaatctgct
    15901 gtaacagaaa catttgccca tgggtttggt acatcataag accaatccca gtctttgaca
    15961 cggtcgttga ctggcaatac ttcattacgg aacaacgttg gaatcgcaaa tgcttcttca
    16021 aatgcatatt gttgccattt ttgatatgct tctttttggt actcttcatc aaatgctttg
    16081 cttgaactga tatcgttgat caattttgta ttttcttctg attcataacg agtgtagttg
    16141 aacgcagcat ttggtcccca tagtccactt ggatttggat cagttcctgt attccaagca
    16201 gcttggtaaa catcaatttc aggatcatcg ttttctaatt tatcatagaa agaattgaat
    16261 tcgatcagac gacctgttgt gtattggaca tccaaaccaa tttctttcca ttgttggata
    16321 tagtaatctg ccaatggttg cgctgtttcc ccaccggaca tcgaagcaaa gttgattgtt
    16381 agtttatttc catctttgtc ttctcttaaa ccatcgtcat ccacatcttt gtagccagca
    16441 tcatctaata gttttttcgc tttgtctaga tcttgtgtga atcctttcat ctcatcatca
    16501 tggtatgact ggaatactgg aggaatcaat gaagtcgcat tgcttctcaa acctgcatag
    16561 aaacgttcac ctactgcatt attatctaat gcatagccca ttgcttgacg taatgattta
    16621 tcagccattt ttgaatctgg attgtattcc acactctttg tatctgcatt ccatttacct
    16681 aacttgaatc ctagataagt ataagaagtt tgttcacggc ctaagtttgt atagccagca
    16741 atttctttat aggtatcgta agtatctgtt ggcatttgga aaatcatatc atataattta
    16801 gagttcaatg cttcagttgc acttgccatc ggtacagatt tcatcgtgat ctttttcaat
    16861 ttaggcgtac cattccagta gtgctcgttt ggcaagtatt ctactgattc tcctgcaacg
    16921 atattactca tgtaataagg gccaaaagtc actggatttt tacgtacttg atcgcttgat
    16981 tccatatctt tgattgctac accttctaac gtatgttttg gtgctgcata tgaccagata
    17041 ccaccacccg attgaagcat gcttgggttt acttctttat attcgatttc aactgttttg
    17101 tcgtctattt ttttgattcc ggagattgtg tcggcacttc catcatgata ttcttccata
    17161 cctacgatgt tacggaaagt gtcgtcataa cggataccag tgtaatcttt attcccaatg
    17221 atttcgtaag ggaaaatcac atcgtccgcc gttacatctt caccatctga ccatttgacg
    17281 ttgtctttta ctttgattgt cgcttttttc gtatcttcat ctagatcaag cgttgcagct
    17341 ccgtcgtctg tgattctgaa atcgtcatcg tttgcaaata gcggttcatg agaaggagcc
    17401 ataaaagctg catcatatga atcttggtaa aactcccact ggaacaatcc tttaaactga
    17461 ctgtccgttg cgaccgcagc ttctaattcg ccgtcttcga ttgcatcatc attatttttt
    17521 gttgcaattg gaaaagttaa ttcctctttg ttattgccgt tgcctccaga gttgtttcca
    17581 gaggtatctg tacctccgcc cccgcatgct gcaagaacga cagcagacat cagcgctgca
    17641 aatccaaaaa tcgttttctt cattcctatt tcccccttcg cttctatact agcctaaccg
    17701 ttggcgcgca tctgcagaac gcttgaatgc ttgtccaaca taattgatac tcaacatcaa
    17761 aactaaaata aggattgatg cgggtaacca gatccattgc cgattcacta agacttctcc
    17821 gttacttgca taaccgatca atgtacctaa actaggtaca tttgccggca agccgaaacc
    17881 taagaaagtc aaagttgtct cgataccgat gtttgccgca aagttcatcg tcaaattagt
    17941 aatgattaat gaacttagat tcggcatgat ttcgcggaac atgattttaa aatcattggt
    18001 tcccattgtt tttgaggcac tgacataatc tcttcttgct tcagataatg tcttgcttcg
    18061 gaataatcgg gctttagcta cccaataaaa tgcactcatg atgaatataa aggaccagac
    18121 attgtatctg gaaataatcg atacgaagac gataatgatc agcatgatcg gtagaatcat
    18181 gataaaatca acgatccgca tcaagatgtt gtcaatgatc cctccgtagt agccagagat
    18241 gatccctaat ccaaccccga tgatcgaagt aaggatcgtg atcgcaaatc cgatcgtaac
    18301 agagtttcta gcacctatga tcaattgtcc taatacatca cgtccgcctt catctgctcc
    18361 cagaatgaac ttttgtcctt gtgtcgaaac tgttcctggt gcagcgtatt tgtcgaagat
    18421 actaacatac atcactttgt cttgatcggt cagcatcgca ccgataaaga tcactaataa
    18481 taaaaccact agcaaaccta atgaaaaaat agcaatttta tcttttttaa attctcgagc
    18541 aatcattcgt atccccatag gaggaatact ttcttgaact gctgtattgt ttttttcact
    18601 catcgatcca ttctcctttc tgattggttt tattgaatcc gaatacgcgg gtcaacgata
    18661 ctcatgatga tatctgaaag taatgtcccc actagcgtcg cgattcctaa aatcaatact
    18721 aaggatgtga tcacggtata gtcccgttgg ccgatggaat caataaatag ttttccgact
    18781 cccggataac caaaaatctt ttcgataact acagaaccag caatcaacga agtgatttca
    18841 taccccattt gagaagccat cggcaacgct gcatttcgga aaatatgtcg actgaatact
    18901 ttattcgtcg gtaccccttt ggaacgggct gttctgacaa aatcgagcga tttcgaatcg
    18961 attacttcac ttcgcagata ttggattgtg acggcagtcg ccaaaaatgc ttgggtaatt
    19021 gatggtaaca gaatatattt caaacggtct atccattgag tgataaatcc accttgcaaa
    19081 cctagtgttt gtgatccact tgttgggaac cagcctaaac gataaccaaa aacaaatagc
    19141 atgatcaagc cgaagataaa taaaggaaca gcaaaactaa agaagttgta gattacgaca
    19201 atcttatcca agattgaatt ttgataccgg ccagcaagca tccccagagg cacagcaatc
    19261 aaataagtaa ggacaactgt caagatcgat agatataaag tgttgatgat ccgttcgccg
    19321 atcaagtcag caacagagcg tttgtataag aaactttgac caaaatcgcc ttgcaaagca
    19381 tttccgatcc aacggaagta ctggacatac catgggtcat tcaagccagc tgcttgtcgc
    19441 atctgttcga tcactgccgg atcctgattt ggattgatca aaccggtaaa tggatccccc
    19501 ggcatcgctt gggctaataa gaagatcaga acacttaaaa taataacttg cgggatcatc
    19561 aataaaacac gtcgcagaat cgttttccac attagcttgc acctccatct ttcaacgcca
    19621 ctttgtgtgt agttgtgatc gtacgcaaat cgtagactcg accagtcgca tcatagtatt
    19681 ctttttggtt ttggatatac tcttgttcta ctcttcgtcg attttctttg tggctttccc
    19741 tattttctac atcgatttgc ggaatagctg aaagcagacg tttcgtatag atatgtcgcg
    19801 ggtcattata aatgtcttct cttgtaccga tttctacaaa tcgacctttg tacataattg
    19861 cgatattgtc gcacatgtgt tttactacac ctaaatcatg tgaaatgaat aaatagctga
    19921 ggccgtattc ttgctggatt cgtttcatga aattcaatac ttgcgcttga acagatagat
    19981 ccagagcaga tacaggttca tcggctacga tcagttttgg attggtagca acagcacggg
    20041 ctactcctag acgttgacgt tgtccgcctg aaaattcatg aggatattta tatagcgcat
    20101 cttccggcat cccaacgata tctagcagtc cttttacctt tttcttttct tcttgatcgc
    20161 ttaaccgttc aaaatttcgg atgggttcag caatgatatc caacactcgc tttttaggat
    20221 tcaaactgga cattgaatct tggaagatca tctgcacatc cttgttataa cccatttttt
    20281 tgcggttgct tcgcttcgtt acatcttttc cttcatacat gatttgtccg gctgttacct
    20341 tttctaatcc cacaacggct tttcccgtag tagattttcc agagccagac tctcccacta
    20401 aaccatatgt ctttcctttt tcgataataa aatccacacc atctacagca tatacatgat
    20461 ccgttactcg attgaagaat ccactgcgaa tagggtaatg aactttcagg tctttgattg
    20521 tgattaattc tgtcatctac acctctcctt tttcgtctct aaagtggaaa tgcttatagc
    20581 aggagcaacg taccaagtgg ttcggtgcga cttcatgaag gataggctct tcttcatgat
    20641 cgcttgctgg tatccatgga atacgtggtg caaaccgaca acctttacga ggcatgtttt
    20701 tcagagaagg aacgacacct tcgatcacat ggagttctgc atcatgattt ccttcttgtg
    20761 gtatagagtt cagtaaagag cgtgtataag gatgcttagg atgatcaaac agttctttta
    20821 cacctgctac ttcaacgaac tgtccgccat acatcactgc cactcgatca gccatttctg
    20881 ctacgactcc taagtcatga gtgatcaaga tgatccctgt ttttgtttct tcctgaaggt
    20941 ctttcagtaa atcaagaatc tgtgcttgga tggttacatc tagggctgtc gtcggttcat
    21001 ctgcaatgac gattggaggt ttgcaggcga ttgcgattgc gatgatgaca cgttgtctca
    21061 taccgcctga tagttcatgg ggatattggc gtccaactct cgcaggatta ggaatcccta
    21121 cttgttctag caattctagt gcacgagctt gacgctgttc tgcattcatc tttgtgtgat
    21181 aggaaagtcc ttctttgatc tggtcttcga ttctcatgag aggatttaat gcagacaacg
    21241 gatcttggaa aatcattccg atatcatttc cgcggatttt attatagagc gtttcgttca
    21301 ggtcgattaa attcagatcg ttatataaaa tctcacctgt aatttttgtg ttattaggat
    21361 caagtaatcc cataatcgta gtagccaagg tacttttccc gcacccagat tccccaacga
    21421 ttgctaaaat ctcgtttttg tctaaagtaa atgaaacgtc atctactgca tcgtaatagt
    21481 catctttgag tcggaatcct gtatgtaggt tctgaacttc taataattgg ttatcggaca
    21541 atcgaatacc cctctctgct tcctaaatgg aagtttttct taataaaaaa ggacattaca
    21601 cagaataaat cgttttataa caaaggttat tcttatcttt gtgtaatgaa ttattaataa
    21661 ttatatagaa aaccgcttta ctttgcaaca taatttctgt ttatcgccag aattatcaga
    21721 aaaatgactt ttacgtgtca aaaagattaa aaaaagaagg ataa
//
LOCUS       DARDHA010000032        21402 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000032 DARDHA010000000
VERSION     DARDHA010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.032"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..736
                     /locus_tag="SDA10_000985"
     CDS             <1..736
                     /locus_tag="SDA10_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4239913.1"
                     /translation="FIRKALQEAVITELISTNVADKVRKPKKKQFVSQVYNQQEILKL
                     LDILSEEKLYLVVLLTAFYGLRRSEVLGLKWSAIDFLNKKIMINHVIIENPENMSQLI
                     KKDQTKNSSSYRTLPLVETIERALVNQAKWQQDNRILLGDDYILEDNEYVFTMEDGRL
                     MKPQYVTHRLSKLIKKNGLKKIRFHDLRHSNASIMLDSGQNMKSIQEWLGHASYSTTA
                     NLYTHLTAGVKDRAAEALENAFGFKE"
     gene            1310..1879
                     /locus_tag="SDA10_000986"
     CDS             1310..1879
                     /locus_tag="SDA10_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEG4239914.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1866..2726
                     /locus_tag="SDA10_000987"
     CDS             1866..2726
                     /locus_tag="SDA10_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG4239915.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2916..3620
                     /locus_tag="SDA10_000988"
     CDS             2916..3620
                     /locus_tag="SDA10_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4239916.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3625..5460
                     /gene="walK"
                     /locus_tag="SDA10_000989"
     CDS             3625..5460
                     /gene="walK"
                     /locus_tag="SDA10_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEG4239917.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5457..6773
                     /locus_tag="SDA10_000990"
     CDS             5457..6773
                     /locus_tag="SDA10_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239918.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6774..7643
                     /locus_tag="SDA10_000991"
     CDS             6774..7643
                     /locus_tag="SDA10_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEG4239919.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7698..8507
                     /locus_tag="SDA10_000992"
     CDS             7698..8507
                     /locus_tag="SDA10_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG4239920.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8610..9239)
                     /locus_tag="SDA10_000993"
     CDS             complement(8610..9239)
                     /locus_tag="SDA10_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEG4239921.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9409..10701
                     /locus_tag="SDA10_000994"
     CDS             9409..10701
                     /locus_tag="SDA10_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4239922.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10777..11439)
                     /locus_tag="SDA10_000995"
     CDS             complement(10777..11439)
                     /locus_tag="SDA10_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG4239923.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11664..11948
                     /gene="groES"
                     /locus_tag="SDA10_000996"
     CDS             11664..11948
                     /gene="groES"
                     /locus_tag="SDA10_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEG4239924.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11999..13624
                     /gene="groL"
                     /locus_tag="SDA10_000997"
     CDS             11999..13624
                     /gene="groL"
                     /locus_tag="SDA10_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEG4239925.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(13713..14861)
                     /locus_tag="SDA10_000998"
     CDS             complement(13713..14861)
                     /locus_tag="SDA10_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4239926.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(14963..15547)
                     /locus_tag="SDA10_000999"
     CDS             complement(14963..15547)
                     /locus_tag="SDA10_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEG4239927.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(15674..16105)
                     /locus_tag="SDA10_001000"
     CDS             complement(15674..16105)
                     /locus_tag="SDA10_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEG4239928.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(16123..16443)
                     /locus_tag="SDA10_001001"
     CDS             complement(16123..16443)
                     /locus_tag="SDA10_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4239929.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            16743..16883
                     /locus_tag="SDA10_001002"
     CDS             16743..16883
                     /locus_tag="SDA10_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239930.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            17181..17438
                     /locus_tag="SDA10_001003"
     CDS             17181..17438
                     /locus_tag="SDA10_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HEG4239931.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(17409..17717)
                     /locus_tag="SDA10_001004"
     CDS             complement(17409..17717)
                     /locus_tag="SDA10_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239932.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            17795..18541
                     /locus_tag="SDA10_001005"
     CDS             17795..18541
                     /locus_tag="SDA10_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEG4239933.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            18556..18759
                     /locus_tag="SDA10_001006"
     CDS             18556..18759
                     /locus_tag="SDA10_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4239934.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            18864..19163
                     /locus_tag="SDA10_001007"
     CDS             18864..19163
                     /locus_tag="SDA10_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239935.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            19163..19345
                     /locus_tag="SDA10_001008"
     CDS             19163..19345
                     /locus_tag="SDA10_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239936.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(19351..19827)
                     /locus_tag="SDA10_001009"
     CDS             complement(19351..19827)
                     /locus_tag="SDA10_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239937.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            19880..20605
                     /locus_tag="SDA10_001010"
     CDS             19880..20605
                     /locus_tag="SDA10_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEG4239938.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            20779..21120
                     /locus_tag="SDA10_001011"
     CDS             20779..21120
                     /locus_tag="SDA10_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239939.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            21113..>21402
                     /locus_tag="SDA10_001012"
     CDS             21113..>21402
                     /locus_tag="SDA10_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEG4239940.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         7240 a         3585 c         4303 g         6274 t
ORIGIN      
        1 atttatacgc aaagctcttc aagaggctgt tattacagaa ttgataagta caaatgtagc
       61 agataaagta agaaagccaa aaaagaaaca atttgtttct caagtttata atcaacaaga
      121 gatcttgaaa ttacttgaca ttttgtcaga agaaaaactc tatttggtag ttcttttaac
      181 agcattttat ggattgcgaa gaagtgaagt tttaggatta aaatggtctg ctattgattt
      241 tttaaacaaa aaaatcatga taaaccatgt aatcatagaa aatccagaga atatgtcaca
      301 gttaataaag aaagatcaaa caaagaactc ttccagctat cgtacattac ctttggttga
      361 aacgattgaa cgagcattag taaatcaagc taaatggcaa caagataatc gtattctttt
      421 aggagatgat tatattttag aagataatga gtacgtattt actatggaag atggacggtt
      481 gatgaagcct caatatgtaa cgcatcgttt gagtaagttg attaagaaaa atggattgaa
      541 aaaaattcgc tttcatgact tgcgtcattc taatgcttca ataatgttag atagtggcca
      601 gaatatgaaa tccattcaag aatggctggg acatgcatcg tattcgacaa ctgctaattt
      661 atacacgcat ttaaccgcag gagtaaaaga tcgtgcagca gaagcattag aaaacgcatt
      721 tgggtttaaa gaataaaaaa acaaaaaaca atatctttgg ggtatcgctt cttgttttta
      781 gaattttttt gttggcaatt tgttggcagt atgttggcaa tgaaaaaatt cacaaactaa
      841 aaacctattt ataaagcttt ataaaacttt tttcacaaat agagcatttg gttaagagtc
      901 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      961 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
     1021 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
     1081 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
     1141 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
     1201 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
     1261 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
     1321 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
     1381 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
     1441 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
     1501 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
     1561 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
     1621 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
     1681 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
     1741 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1801 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1861 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gttccagaag
     1921 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1981 tatatacaga taaaattgac attacccctg aagagatcta tcaacgtttg ccagaggaaa
     2041 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     2101 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     2161 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     2221 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     2281 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     2341 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     2401 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     2461 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     2521 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     2581 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     2641 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     2701 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     2761 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2821 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2881 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2941 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgacgttt
     3001 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     3061 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     3121 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     3181 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     3241 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     3301 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     3361 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     3421 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     3481 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     3541 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     3601 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     3661 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     3721 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3781 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3841 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3901 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3961 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     4021 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     4081 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     4141 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     4201 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     4261 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     4321 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     4381 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     4441 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     4501 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     4561 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     4621 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     4681 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     4741 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4801 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4861 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4921 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4981 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     5041 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     5101 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     5161 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt gattttaagt
     5221 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     5281 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     5341 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     5401 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     5461 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     5521 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     5581 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     5641 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     5701 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     5761 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5821 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5881 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5941 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     6001 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     6061 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aagaagtata
     6121 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     6181 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     6241 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     6301 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     6361 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     6421 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     6481 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     6541 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     6601 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     6661 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     6721 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6781 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6841 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6901 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6961 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     7021 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     7081 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     7141 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     7201 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     7261 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     7321 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     7381 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     7441 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     7501 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     7561 aagtggtcca aattgagagc gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     7621 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     7681 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     7741 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7801 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7861 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7921 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7981 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     8041 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     8101 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     8161 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     8221 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     8281 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     8341 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     8401 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     8461 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     8521 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     8581 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     8641 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     8701 gtactgtcgc tagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     8761 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8821 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8881 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8941 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     9001 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     9061 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     9121 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     9181 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     9241 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     9301 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     9361 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     9421 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     9481 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     9541 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     9601 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     9661 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     9721 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9781 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9841 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9901 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9961 gcggtttttc aacatttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
    10021 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
    10081 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
    10141 gtcttgcaag cgaattcatt agcaaatgca gaaatcttag cctcaggaca agagttgacg
    10201 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
    10261 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
    10321 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
    10381 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
    10441 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
    10501 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
    10561 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
    10621 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
    10681 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
    10741 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10801 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10861 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10921 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10981 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    11041 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    11101 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    11161 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    11221 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    11281 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    11341 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    11401 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    11461 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    11521 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    11581 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    11641 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    11701 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    11761 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11821 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11881 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11941 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    12001 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    12061 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    12121 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    12181 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    12241 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    12301 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    12361 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    12421 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    12481 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    12541 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    12601 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    12661 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    12721 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12781 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12841 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12901 cgatctaggt ttagaattga aagatgcaac aatcgaaaac ttaggaaatg cttctaaagt
    12961 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    13021 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    13081 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    13141 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    13201 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    13261 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    13321 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    13381 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    13441 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    13501 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    13561 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    13621 gtagtaatta agaaagaacc ctattttaac agggttcttt cttctttttt tgttttattt
    13681 taagcaaaag gggcaaagaa ggggcaaaaa tatcaaaggc tatttagttt atcaattact
    13741 tgttgtttgg ctctttttgt tacatggtta taaatagaca gagttgtgtt tgcatcagaa
    13801 tgccctacac gctccataat ggctttaaga ggtacgccta attcagataa tagtgaaacg
    13861 tggctatgtc tgaatatatg tgaggataga cttttttcta attccaactc ttcctctact
    13921 ttatggagta tagcgttaaa tgagtgcagc gcaagcggag tgccacttgt agatataaat
    13981 atatattgat cgggatctgt gggtctacct gcaagaatat tgtcagctat cacactttca
    14041 atcaattctt ttgcacgatt gggtaattgc acttcacgtt gcgaataagt attttttgga
    14101 gttgttttta tagcattatc cattttcaca gatgtgtaat ctaaggtccc attaatggaa
    14161 attttcccat cctcatagtc cttcatttgc aaagctagca attccccata tctcaaacca
    14221 gttaaatata gaaactcagc tattatgccg tgtagttttc tgcgaggatt ggagtatagc
    14281 tgtttcagta tttgatcaat ttcttctttc tccagatatt ttttattcat agaaagcctt
    14341 cttttttctt cttccacttt tttaggatgg attttaactg ctagcgcagg gtttctttga
    14401 atgtattttc tatcgattgc atagtttagc ataacagata gagttgtttt tgtttgtttt
    14461 gtgtagttca gtgagaggtc accaaacgta tacatatctt caattatctt attaatgagt
    14521 gtctcatcaa tgtttctaac gatcgtatca tcgcttatgt gcttagaaac atgtttcatc
    14581 atcattggaa ccttcaaata gctagtacgt ttaacatgct gcttataata ttcataccat
    14641 tctttataca gctcaccaaa agtgatatct gatttattgt aatcttcgag tgcttcttta
    14701 atttttttat ctagaatttt ctgagctttt ttccacgctt gtggtgaatt acttgtaagt
    14761 gttgtagatt tttttcgtgt tttttctgta taaggatcta catatctttc aataaacttg
    14821 aattggccat ctttggtctg ttcaacccac acttttaaca tctcctatca tttgctataa
    14881 taggcataac aaatagacct atataggttt gttttctaaa agcacgctct tactctggac
    14941 ggtggggcgt gttttttatt tattatacag ttgctttaaa aatagctgct ttttcaaatg
    15001 atactaaagg agagaagtgg atttctattt ctcccttttg gtttaacgtg aaatgagcag
    15061 ttacatccat ttgttttcct ggagcaacag atcccattgt attttcgttg gcatatgtct
    15121 ctgatttttt gtcatctggt ccatatactt ccacatctgt acctacaggg atatctgaat
    15181 cgccatcatt ttttacggta taagtaattt ttactacttg tgcaggttgg ttttcttcaa
    15241 attgatttct ttcatcagtt agttctacac tatttagtgt atattcagca tcaccaactt
    15301 ttacagtgtc accaatctta tagaaggtat cgcttttttc ttctttagaa gatgaagcag
    15361 aggttgaaga tttagttact ttttcgccac cattatcact tgctttatta ctatctgatc
    15421 caccatttaa agcagaacca ataattataa ttaaaattac tgctaatacc caaaaccata
    15481 cttttttgta aaaaggttta ctaactttat acatttttcc gtcttgaccc ataacttttt
    15541 ttgccattta aatattcctc acttcttgtt ataatatatt tgcgatctca gaaatgaggt
    15601 atgagtccgt gtgccagcac gggctttttt aatgttttgc tgaaatcggt ttttttaata
    15661 attcttgatg tattcataca tattgccttg cgtaagaata ttttttctta aaatagcatt
    15721 ggcagacagc ataacaagtg tatctgagct tattctgaat agaataatac tccataaatt
    15781 tttcgagatt aaactgagat tcatcagtca gttcgttctc aatatagata tttaataaaa
    15841 ttagaatagc tattttatct gcttctgttt caaattttga gtgaaaagtt gtagaagtat
    15901 cgtacaaaac tgaatattca aaatgagaag cggtgaaatg cgcaagttca tgagataaat
    15961 gaaaggcttc tgcagtttcg ccatatagat tttcattcaa aaaaatgatt ctaggcttcg
    16021 gataatagaa gccaggctct ttcatttcca tgtaaactac tttcaaattg tattcgctca
    16081 acatttcttt caatttcaaa tacatacaac ccatcactcc aactattcat tttcctctaa
    16141 agctttagca attgcaatcg ctttacgcat tgtctcctta gatatttctt ttccatcaaa
    16201 agaaaaaaca gtatcgtttt ctgataaatc cacatgttta ggggtttctc tttcttctct
    16261 acctagaagg tagtctacag agactccaaa gtaatcagct aaccgagcta actcaataga
    16321 attaggagtt tgtcttttcc aacttcctaa atatccattc gaatacccga aattcatttc
    16381 gagctgtcta atggataatc ctttttcttt tgctaactcc tttattattt cgtaagtatt
    16441 cattgataaa tcaacctttc tgaatgctta caaaaaaagt ttagataaat aatgtaaaaa
    16501 tagttgactt atatacatta ttaatctata ctatgttttg taaacaagtt taacaactaa
    16561 aaagacaaca aaaaatacta ttgattaata aatgccaacc gccaagaaag ctttttaaat
    16621 caaagtttat atgtcttatt taactatgca tttagtatag attattaatc taataaagtc
    16681 aacgcagttt tttaaaaagt tgttaaattt gtttacgaat ataaaagaaa ggagagaaga
    16741 atatggaaaa aacagtctcg gcaaaaatcg aagatttgaa attagacata ctgaaacaag
    16801 caaaagtggc gatggaacac gcggtagata aagaagactc tgccatggtt gcagccatag
    16861 cagagatttt agctcacgtt tagtcatcgt ttttacggtc ttctggaagc tgaccataga
    16921 taacagcata atgaatatct aaataagctt gaacaatttc ttttggcgaa attgattcgc
    16981 ctttagtaga tacttgtgat tcgtgattta gccatgcaac aactaaatcc gcagcgattt
    17041 caaacggcgt tacactttgt gtcagttgcc ataaagaatt tcacaatatg tatggtcgtg
    17101 gtaataatac caaagagcaa tttgaggaat ttatggaaat taaaaaatac aaaaaatcat
    17161 ttattaaagg aggctaaaca atgaacaagg aaatgagcca aacaatcaag gtacaaaaaa
    17221 tgatagatga tctaacacat ggaattgata gccaagccga taaaatcatc aaggaattgc
    17281 aaggacaaaa agttaaggat gcaaaaatgc ttctcaagac tatcaacttt gaaatgaatc
    17341 caacaaaaag aaagctcgcc gatgtgttgg aagaaaagtt ggcttccgct atcaacgagc
    17401 aagaattact atttgaaacc gatactttta acagctgatg ttttatagat gacagtttca
    17461 tccgaatcta tatcatcgcc gagagaaaag aatgttgctt ttgacaaaaa gccgataaga
    17521 cctacaagcg gtcttgatgt tgcaagagta gataccccaa tatcaccttg caaggtattc
    17581 acgtaaattt gagaagaata gaacattcca tctttttctt cgatatttga cgcatcatcg
    17641 tatccaacga ccaaagtatc ggcaaatatt ttgatttttt ctccatcaag catagtaatt
    17701 acaaattctg gtttcataaa ttttcacctc cttaacaatt atttcagcct gtcacactga
    17761 taaggaaatt ataccaaaga aaggaatgag aaatatgaat acaccacaaa tttttaattt
    17821 cgagcaaaac gaagttcgaa ctattttagt aaatgatgaa ccatattttg taggaaaaga
    17881 tgtagcagac gttctaggat attcgaatcc gcaaaaagct attcgagatc atgtggactt
    17941 ggaggataag acgcagaacg attcgttcac cgtcaatgga acagcagttg ttttgattaa
    18001 cgagtcaggc ctttacagtt taatcttaaa atcaaaactt ccctctgcca aaaaatttaa
    18061 acgttgggta acgagtgaag tccttccaac aattagaaaa acaggtagct attcaaacgt
    18121 acctcaaagt tttgcacaag cattgcgttt agcagcggat ttagaagaaa agaaccaatt
    18181 actcgaacaa caaattgccg agtacgaacc aaagattagc tacttagata cgattctttc
    18241 atcgacagat acggtagcta cttctcaaat tgcagctgat tacggaatgt cggcaattgc
    18301 tctaaacaaa ttgctaaacg agttaggtgt tcagcataaa gttagcggac aatggatact
    18361 ttaccgaaaa catatgaacc aaggatacac aaaatcgcac acaagtgaga taccgaaagc
    18421 cgatggcggc actaaagttg taatgaatac caaatggaca cagaaagggc gagtgtttat
    18481 ttacaactta ttaatcgcag agggctatta ccctcaaatg gatttagagg aaattggtta
    18541 gaaaggagtt ttagtatgac tgacattgca gaaatcactc aacgagatag agaaaaaatc
    18601 aaagaatatg tcgaaagttc aaagttctta acttacacca tgcttgctga aagatttgga
    18661 attagcaaaa gctacttatc tttaatttta aacggtaaaa agacttctgc agaagcaaac
    18721 agaattatag attcgattat cactatgtac gaattgtaga gggaggtaaa gtcgttggaa
    18781 gataaaatca ttcttacaaa atcagagctt caagaaatgt taaacatcga gtatggaacg
    18841 ataattttta ggagggataa taagtgaaaa aaccaacact ttcagagctg atagaagctg
    18901 ctgagaaggc agtaaaacca gacgactggt accgacaaag tttaatcttg gagaagttcc
    18961 acggcatgtc aaaaactact ttagttgaat actgcaagga aatggaaaca attcctgaat
    19021 ttgcagaagg aatacttcgt ccaggtcact caaccacatt tattcattac catactttta
    19081 tttggttttt aaagtggaaa gacgcaaaca aatatcgtgt aaaaagttta tcgccttcag
    19141 atgttttgaa ggaggcaagt taatgggcaa attcaacaga gcactagtat tcagcgcacc
    19201 gctaatcatc tacgctttag gcctctgggg aagcagacaa gcgttgatag ggacaatcgt
    19261 ttacatggtt tggattttta tggggcttga tgaagctgag cacagagcga aaaaagccag
    19321 tcgggaggga ctgactaaaa tatgattttt ttatttttgt tcctttgggc tgctattgcg
    19381 tacttcatcc agctccttgt tgtcaaagtt gctcttattt cttttaggaa gaacaaaaga
    19441 aaatagcgct ataaatgaaa gataagtagc cgctacatta aactgttctt ttgaaaaagt
    19501 tacagtcatc ggaatgataa ttgatattga ggaagtaatc ttaattaaaa atatagcaat
    19561 attttcaaca tcgcgttggt ttttatttaa cattgtccaa catgttttag caacaataaa
    19621 aataattaca catgatatta gcacacagac tgaaaaaagt tttagaaatg cgatgtagtc
    19681 agtgttattg aactccttca aaatactatt tttattcaat gtaataaaag gaattttttt
    19741 aattgaagac aggaaagata aggtaataaa cagaattata tatatggtta aagtcgcagt
    19801 aagaatgtaa ttgagctttt tcctcataga atcacctcac tttctaacga aaattatacc
    19861 aaaaaggaga gaagaaataa tgcaagaatt agtaattttg aaaaataaag aagctgtaac
    19921 tacgagctta caagtggcag aaagtttcaa aaagaaacat aagcatgtac tagaagcaat
    19981 tgaatcaata aaaagatcgg tcgaaaattc ggccaatgtt gaagatgggt ccaattttgg
    20041 acagatgttt gtggaaggga acgagccaga ctcatacgga agaagtcgga gagtttattt
    20101 catgaataga gacggatttt ccttgctagc tatgggattc actggaagta aagcaataaa
    20161 tttcaaacta aaatttattg aagctttcaa cgaaatggaa gatgttattc ggaagaatac
    20221 tgttcctcaa acaattgaag acatgatgat ctatcaatta gaagaaatga aagatgttaa
    20281 aaaagatgtt tccatgctta aagatactat gcgaattagc ggacaacaag agtttgaaat
    20341 taagcaaaaa ggaaatatga aagttatgga agttctaggg ggaaaagaaa gccgagctta
    20401 tgaagaaatc agcaaaaaag tattctcaaa attttggtct gaatttaaac gtaccttttc
    20461 aatcccaaga tatggcgagt tacctcgtaa gagattcgat gatgctgttt catttattga
    20521 aatgtggtta ccagaaactg cgatccgcat ggaaatcgat caactgaaca gacaacaaag
    20581 acttttcggt gatgaaaatg aatagagctg aagcgctaag aatagggacg gtaattgcta
    20641 atcgctggtg gagacacaat aaaccaaaca tcctaagcca acaacatatc gaaaagcaaa
    20701 aagcatggca acaaataaaa aagtgactca gccggcaagc atagagtcac aaaacatatc
    20761 taaggagaat tttagcatat gaataaagaa ctttccactt tagatcaata tttgactgat
    20821 tctgaatggg gcaagtcgaa tatcaaggaa acaaataatc gaaaaatcag acgaaatctt
    20881 ttgacgaacg aagaactatc atgtgatcaa gatgacttgg gaaattttgt gagtatttgg
    20941 gatcatgttt accttatcca tctatcaaaa cattcaaata aacctgaata catctatgtc
    21001 atcgaagatg gcttgattga tgcgctagaa gagtacaaca gagataactt gattgatatc
    21061 tcttattacg gatcaggtaa gaaatacatt gctgaaatgg aggcagaatt tgatgagtga
    21121 aatcaaaggg acaacgaact ttgaaaaact ttttagtcgt aagttaaata aaattctcaa
    21181 gaaaaaagga aattttgatt atttatcttg ggctcacgcg tgggagatta tgaaaaagaa
    21241 tgatccacag gcaacggtaa ctattaatga gtataaacac tacagggttg tttctggaac
    21301 tcatcaagac tttcttgttg aggaatataa accttttctt atggatgaaa cagggactta
    21361 tgtatctgtc tcagtaacgg ttaaaggaca tacggaaaca ga
//
LOCUS       DARDHA010000033        20766 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000033 DARDHA010000000
VERSION     DARDHA010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.033"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            12..1133
                     /locus_tag="SDA10_001013"
     CDS             12..1133
                     /locus_tag="SDA10_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEG4239941.1"
                     /translation="MVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRINKVPMPS
                     AGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPKQKSIGI
                     LIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDGLDGLAA
                     GISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLGDTGALF
                     LGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTADKKHLH
                     HRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAILLFELIG
                     LINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            1126..1926
                     /locus_tag="SDA10_001014"
     CDS             1126..1926
                     /locus_tag="SDA10_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG4239942.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            1926..2753
                     /locus_tag="SDA10_001015"
     CDS             1926..2753
                     /locus_tag="SDA10_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG4239943.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            2731..3465
                     /locus_tag="SDA10_001016"
     CDS             2731..3465
                     /locus_tag="SDA10_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG4239944.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            3565..4431
                     /gene="rfbA"
                     /locus_tag="SDA10_001017"
     CDS             3565..4431
                     /gene="rfbA"
                     /locus_tag="SDA10_001017"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEG4239945.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            4445..5017
                     /gene="rfbC"
                     /locus_tag="SDA10_001018"
     CDS             4445..5017
                     /gene="rfbC"
                     /locus_tag="SDA10_001018"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEG4239946.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            5039..6067
                     /gene="rfbB"
                     /locus_tag="SDA10_001019"
     CDS             5039..6067
                     /gene="rfbB"
                     /locus_tag="SDA10_001019"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEG4239947.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            6165..7016
                     /gene="rfbD"
                     /locus_tag="SDA10_001020"
     CDS             6165..7016
                     /gene="rfbD"
                     /locus_tag="SDA10_001020"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEG4239948.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            7051..9084
                     /locus_tag="SDA10_001021"
     CDS             7051..9084
                     /locus_tag="SDA10_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239949.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            9128..10408
                     /locus_tag="SDA10_001022"
     CDS             9128..10408
                     /locus_tag="SDA10_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HEG4239950.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            10618..11424
                     /locus_tag="SDA10_001023"
     CDS             10618..11424
                     /locus_tag="SDA10_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4239951.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            11436..12656
                     /locus_tag="SDA10_001024"
     CDS             11436..12656
                     /locus_tag="SDA10_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4239952.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            12646..14232
                     /locus_tag="SDA10_001025"
     CDS             12646..14232
                     /locus_tag="SDA10_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG4239953.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            14271..16409
                     /locus_tag="SDA10_001026"
     CDS             14271..16409
                     /locus_tag="SDA10_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG4239954.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(16777..17760)
                     /locus_tag="SDA10_001027"
     CDS             complement(16777..17760)
                     /locus_tag="SDA10_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEG4239955.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            18138..19529
                     /locus_tag="SDA10_001028"
     CDS             18138..19529
                     /locus_tag="SDA10_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEG4239956.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            19545..20585
                     /locus_tag="SDA10_001029"
     CDS             19545..20585
                     /locus_tag="SDA10_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG4239957.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            20604..>20766
                     /locus_tag="SDA10_001030"
     CDS             20604..>20766
                     /locus_tag="SDA10_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEG4239958.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIER"
BASE COUNT         6648 a         3444 c         4277 g         6397 t
ORIGIN      
        1 tttcttttag tatggttata agattttttc tgacaatttt attatcactg atattaacac
       61 ctgtattcaa aatcatttca gtacaaacag gtatggtcga caaaccaaat gaaagaagaa
      121 taaataaagt tccaatgcct tcagcgggcg gactgcctat ctttatttca tttgttatat
      181 cgactttatt cttattccgt aaaattatcc ctcaatcgta tattgtaccg atcttgattg
      241 cttctttagt aatcatagtc acaggagtgc tcgatgataa atatgagctg acacctaaac
      301 aaaaaagtat agggatcttg attgcttctc ttattattta ttttgtagca gatatacgta
      361 ttgattcctt cacgcttcct tttattggat atatccaact aggctggctt agttttcccg
      421 taacgatttt ttggatcttc ggtatcacga atgcggtcaa tctgattgac ggtttggatg
      481 ggctggcggc agggatttca ttgatcggtt taattacgat aggaatcatt ggatattttt
      541 tcctccatgc ttctacagtg tatattccag ttgttatttt ctgtcttgta gcaagtatta
      601 tcggtttttt tccttacaat ttttatccgg caaagattta tttaggcgat acaggagctc
      661 tattcctagg gtttatgatg gccgtccttt cactacaagg gctgaagaac gtgacttttg
      721 tttcatcgat ttctttgctg atcgtcatgg gagtgccggt aacagacacg ttttttgcta
      781 tcattaggag aaaagcaaac cgtgtttctt tttctacagc agataaaaaa catcttcatc
      841 accgactaat cagtttaggc tttactcata aaggtgctgt cctgacgatt tatgcgatgg
      901 ctttgatgtt ttctttcaca gctatggtca tgaattatac tggaagaatc ggaacgatcg
      961 tattgattat tgccatgttg tttgctgcaa ttttattatt tgaattgatt ggtctgatca
     1021 atgagaaaca tcagtttatt ttaaaaacat tgcgattttt agggaataaa gagtaccgta
     1081 cgcaagtaaa acagaaatat ttgaagaaat ggaaccgtca tatagatgaa taacaaatta
     1141 aaccagccga tccttattag tattgtgacg gcaaacagtg ataaaatttt tcggacacta
     1201 gataatttta tttcaactgt aaaagatgaa acagcagtaa aaatccgaat ttttgacaac
     1261 aattcacttc ccgagtacaa aaaaagacta acagagtata ggaaatatcc ctacatcgat
     1321 atctattttc atgatgaaaa ttccggattt ggttatggac ataatcacaa tctgatagct
     1381 agcgattctc gctatggcat tatttgtaac ccagatattt tagtggatga agagacagtt
     1441 acagcattgg tcttgttatt gaaagaacat ccggaatgtg cgatgttagc acctaagatt
     1501 cttaatgaag atggaacaac tcagcacctc atccgtgagc agctggctgt ttttgattac
     1561 gttttgcgct ttgtgccttt caaatgggtc aaaaaaaagt ttgacaaacg actggctgtg
     1621 tttgaatgcc gaggattgcc tactgatgct aatagttatg tacggatgat ctcaggtagt
     1681 tttatggtag ctgatattga taaatttaaa gagataaagg ggtttgatga gcgtttcttc
     1741 atgtactttg aagacaacga cttgtgtttg agttttgaaa aagcaggtta caagttattg
     1801 tacacaccct tttatgcagt tatccatttt tacgggaagg gtgcgcatcg aaatgcaaaa
     1861 ctgtttcgcg tgtttttgac ttctatgatg aaatttttca ataagtgggg gtggcgtttt
     1921 ttctgatgaa accagaaata ttagtagtcg ttgtgcttta tcagcaacta ttcagtcaat
     1981 ctccagctta tggaccatta agcaaagcac ttagcgaaaa gcaaattcag cttattattt
     2041 acgataatag tcctcaaagg cagcaaaatg agctgtttga aaaagaaaat atcgtttatc
     2101 atcatgatcc gcaaaatcct ggtttagcgg tggcttataa ttttgcgttg tctcaagcag
     2161 atgaaggaac acgttgtctc gtcactctgg atcaggatac gaggctagtc gacgattatt
     2221 ttgaactatt gcgtaaagta acctttacag atgaatgtgt tgctgcagtt ccgatgattt
     2281 tttctgggag ccgacaagtt tctcccctat attcagatcg ttatatcaat cgtacagctc
     2341 aagcagtaga agttgaaggc ccaacagctg aacgaatcat ggcaatcaat tcaggtgttg
     2401 cctggtctgt caaatttcta aaaaagattg gcgggtttaa tcctgctttt tctttagatt
     2461 ttttggatca ttggctgttt tggaaagtgg atcagttgga aaaaactgtg gagatccttc
     2521 ctgtacgaat ggaacatgat ctttcggttt tagactatga aaaagtttca agtaatcgat
     2581 accgttctat tttacaagca gaaaatcgat tttatcatga atatgatcaa aggcatttat
     2641 ccagtcaccg acgacaatta ttgttaagaa cagctaaaca gttcttgacc gtcaaaaacc
     2701 ggcatatctg gcggatgact ttgcgctcat atgttaagaa ttggaaggtg tgaattcgtg
     2761 cgttcggttt gtatagctac ctttaatgga ggcagatatt taagagagca gttagatagt
     2821 attttatctc agctttcagc agaagatgaa gtagtcattt ctgatgatgg gtcagtagat
     2881 gatacatggg atatattaac tgaatacgca gaaaaagata agcgaatcca gcttttttcg
     2941 gggcctcgtc aaggattgat cgctaatttt ggttatgcga tccagcagac gaaaggagat
     3001 ctaatctttt tggcagacca agatgatgtt tggttggaga caaaagtgaa aaagatcact
     3061 gactattttg agcagaatcc ggatcagcta gtagtagttt ctgatttaat cattgtggac
     3121 agcgagctgc aagagatcca tgattcgtat ttttcgtttc gaaattccaa agaaggatgg
     3181 cttaataatg tgataagaag tcactatatt ggtgctggta tggctttccg gtcttcttta
     3241 aaacccgata ttttgccgat cccgtcagaa gtgccgatgc atgatatgtg gattggtttg
     3301 ctgggaggaa agaagactgg gttcctaaga gaaccgttaa ctctgtaccg aagacatgat
     3361 ttaaacgcga gtgagattga aacacattct aaattacggc aaaaaatttc ttggcgtgtt
     3421 catctgatta cggcgttgat caaacgaaaa tggtttaaac gttgataaaa gatgtttaaa
     3481 tccttaatat ttccatgaca aatataacaa ttgatgagat aataacaaag tgaaatttga
     3541 aatcaaaaag gagagcagat atccatgaag ggaatcattc ttgctggggg cagcggaaca
     3601 cgtctgtacc ctttaacaaa ggcaacatcg aaacaattga tgccgattta tgacaaacca
     3661 atgatttatt atccaatgtc tactctgatg ttggcgggaa tcaaagaaat tctgattatt
     3721 tcaacgccac aagatacacc acgtttcaag gaattatttg gaaacggaaa tgatttagga
     3781 ctgcagatcg aatatgcagt acaagaaagt ccagatggat tggcacaagc gtttatcatt
     3841 ggagaagaat ttattggaga tgatagtgtc tgcttggtat taggggacaa tatttactat
     3901 ggtggcggtc tgtctaaaat gttgcaacga gcagcttcaa aagatacagg cgcgacagta
     3961 tttggctatc atgtcaatga tccagaacgc tttggtgtag tagaatttga tgagaatatg
     4021 caagctttgt cgattgaaga aaagcctgct cagccaaaat caaattacgc tgtaactggc
     4081 ctttattttt atgacaatga ggtaatttct atcgctaaag gtatcaaacc ttcagaacgt
     4141 ggagaactag agattacgga tgtcaataaa gcatatttgg ataaaggaaa actatccgta
     4201 gaacttatgg gacgcggatt tgcttggctt gatacaggaa cacatgaatc attgcttgaa
     4261 gcatctacct ttatcgaaac aattgaaaaa cgtcagaact taaaagtggc ttgtttggaa
     4321 gaaattgctt accgtatggg ctacatcgat aaggaacaat tgctggccct tgcacagcca
     4381 ttgaaaaaaa atcaatacgg tcaatattta ttgaatttgg ccgctgaata aggagtcctt
     4441 acagatgaaa gtaacagata caaaattaac agatgtcaaa atcgtagaaa tggacgtctt
     4501 tggcgatcat cgcggtttct tcacagaaag ttattcaaaa gcaaaatttg cagaacatgg
     4561 attgaaatat gactttgttc aagacaacca ttcactatca gctgaagctg gtgtccttcg
     4621 ggggcttcat ttccaaaaag gagaagctgc acagacaaaa ttgattcggg tagtcactgg
     4681 agcagtttta gatgtagtag tggatatccg caaaggtagc ccgacttatg gtcaatggga
     4741 cggatttatc ttatctgaac ataatcatcg acagttgctt gtacctaaag gatatgccca
     4801 tggattcgtt actttgacac caaatgtgaa cttcctttat aagtgtgata attactataa
     4861 tgcagaagca gatgggggca tcgcatttga tgatccagat ttagcgattg attggccaat
     4921 tgatccatca agagcgatta tgtcggaaaa agatcaccgt catccgacat taaaagaatt
     4981 tgaagcagaa aatccatttg tttacggaga aatctaagaa ttaaggagaa attttcttat
     5041 gaaaaacatc attgttaccg gaggtgccgg attcatcggc tctaatttcg ttcattacgt
     5101 ggtaaacaat catccagaag tgcatgtcac tgttttggat aaactgactt atgcaggaaa
     5161 caaagagaac ttggccggac ttcctagcga tcgcgtagaa ttggtcgtag gagatgttgc
     5221 agatgcagaa ttagtggacc gtctagtaaa agaagcggac gcagttgttc attacgcggc
     5281 agaatcccat aacgataatt cattaaaaga tcctttccca tttgtacaaa cgaatatcat
     5341 tggtacgtat acacttttag aagcttgccg caagtatgat gtacgctatc atcacgtatc
     5401 tactgacgag gtttacggcg atcttccatt acgtgaagac ctgccaggac atggggaagg
     5461 cgaaggagaa aaattcactg cggaaacacc atataatcct tcaagtccat attcttctac
     5521 aaaagctggt tcagatctgt tagtaaaagc atgggtgcgt tcttttggct tacgtgcaac
     5581 catttcaaac tgttcaaaca actatggtcc ttatcagcat attgaaaaat ttattcctcg
     5641 ccaagtaacg agtatcttaa gcggtattcg tccaaaatta tatggcgaag gaaagaatgt
     5701 tcgtgactgg atccatacga atgatcattc ttcagcagtg tggctgattt tgacgaaagg
     5761 cagaattggc gaaacctatc tgatcggtgc agacggtgaa gaagataata agaccgtgat
     5821 ggaattgatc ttagaaatga tgggccaacc aaaagacgca tacgaccatg taaacgaccg
     5881 cgcagggcac gatttgcgtt atgcaatcga ttcgacaaaa ttgcgagaag agctaggttg
     5941 gcagcctgag tttacgaact tccgtgatgg gttggctgac acgatcaaat ggtacgaaga
     6001 acatgaagac tggtggaaaa aagaaaaaga ggccgttgaa gcagcatatg ccaaaaatgg
     6061 acaataatac agaggaaatt tcactgtatt aatggaaaaa cacatgtgag gcaggcgctc
     6121 gcctgtctct tttttaaaat aaaaatcaag gagcggataa ccatatgttt ttattaactg
     6181 gaggaaatgg acaattaggt acagaattgc gccatttatt agatgaaaaa aatctgccat
     6241 atgtttcaac agatgcaaaa gacttggata tcacagatgc tgagaaaaca atggcttata
     6301 ttacagaatt aaaacccgat gttattttcc attgcgcagc ttacacagca gtagataaag
     6361 cagaagaaga agcaaaagaa ttagatgaga aaatcaatgt cgatggcaca agaaatgtag
     6421 cacttgcagc aaaagctgtg aatgcacaat tcgtctatat cagtacagac tatgttttcg
     6481 atggaaaaaa caaaaatgta gaatacgaag tagacgacca aacgaatcca ctaaatgaat
     6541 atggtcgtac gaaattatta ggcgaaaaag cagtagaaga gattttagaa gactattata
     6601 tcatacgaac ttcttgggtt ttcgggatct atggacacaa tttcgtcttc acgatgcaac
     6661 gcctagcgga aacccgcgac aaactgactg tagtaaatga ccagtttggg cgaccaactt
     6721 ggacacgtac tttggcagaa tttatggtat atgtcatcga agaaaaagca ccatttggta
     6781 tttatcattt gtcgaacgaa aatagctgtt cttggtatga gttcgcaaaa gaaatattga
     6841 aagatacaga tgtagaagtt gcacctgtta cgtcagaaga atttccgcaa aaagcaacac
     6901 gtccgcagta ttcagtcatg agcctgaaaa agacagaagc gttaggtttt gtcataccaa
     6961 cttggcaaga agcacttgcg caaatgctgg aagctgccaa aaaaatgaat aagtaatttc
     7021 ctttttatca aatagaattt ggagcatgat atggaaaaac aaacaagaag tgcacgacga
     7081 aaagaaaagc caaaagcgaa tcaggcagat tcgaatttgg tgcttgacct gtttctaaaa
     7141 ctccgctatc ttattggcgt tttagtcatc gtggtcatcg ttacttttaa tttaaacgga
     7201 agttcacttg gtgtttggga taaatatgtg tcgcaaagag acgatgggaa aaaaactgat
     7261 gtgatttttg gacagaatcg ggaagtgcgg tcagacgaat ggctggtgca gacacctttt
     7321 tacctctcac aagctgaaga ggacttttcg ttggtgaacg aggattattc attaaacgga
     7381 caaaacatga tcattgcgta taattctcct gtaaaggata ttacggttat cggaaaacca
     7441 ttcaactggg gattcttttt cctcggaaga gatcgaggac tttcctttta ttgggcgatg
     7501 aaattagtcg tcatggtctt gcttggattc gaagtactga tgatattaac gaagaggaaa
     7561 aaagctctat cgctaatcgg tgcatttgcg ctgacatttt ctccagcagt ccagtggtgg
     7621 ttcatgcaac atgtgggcga tttgattttc tttactttgg gactgatgat tgctttttat
     7681 cattatttct acaatcatga taaaaaatgg ctacgaacgt tgatgatgct tttggtggtt
     7741 attttcggta ttgggtttat tttagtgatt tacccagccc atcaagtgat gctggcgtac
     7801 ttgctgattt tttattttgt tggcctgctt atttactata gcaagaaagt ttcatgggat
     7861 ctgttggatg tagtgttgat tggaggggca gttttattta ttggcggagt catggttcat
     7921 ttctggctaa cgtcaaaaga cgcattgtta gcttctttga ataccttata tccaggaaaa
     7981 cgggtaagta ctggaggaaa gtggacgatt ggcgaatttt tttctttcct tacaaattgg
     8041 aaaattcctt ttgaagatat caactattcg aataactctg aagtggccct gttctatcac
     8101 ttttttccaa ctgtattcct agcaagtcca tttgtgttat ttggcaaaaa agatagcgaa
     8161 caaaagttac tgggacgtat tttgatgatt ttttgtctgt tttctatctt ttggattacc
     8221 gttgggttgc caaaatggct agcagaaatc actctgctgt cttatgttcc tccattaaga
     8281 gccatcctta cttttagttt tgcggcttgt ttattatcga tctggtttat cgcttatatt
     8341 tggagagaag atgttattcc tatttggtat aaattcattt tgttggcagt aaatgcatgg
     8401 tcttatttct atgcgattac gcattcgaaa atggataatt atttttcgga ttttgaaacc
     8461 atcgcagttg ctgtgttggg aacagtgatt ttagcttttg cattatttgg ctggagacgc
     8521 ttgttttacc tcgcttttgt cgccttagtt atcgtatctg gctttttcgt caatccagtc
     8581 gtagaaggta caggagcgat ttttgaaaaa acactggcaa aagaaatcca aaaaatcgat
     8641 caacaagatc ctggtaaagt ttggttgtct gaagatgaat tatataactt caccccaaca
     8701 ttgggagtga aatccttcaa tacagtacgc ttttatccag atatagctgc atggaagaaa
     8761 atcgatccga aagaagaata cgagaagtat tataatcggt atgctcatac aagagctttt
     8821 atcgctaagg aagatacaaa gttcatttta gatcgtccag atatgttttc ggtcacgttg
     8881 aattttgaag acgcagaaaa attagggatc gactatgtga tcagtaaacg accattaact
     8941 gattacaacc aattatatga agcgcagttc acgcagctat atggaccgga taaagacggt
     9001 tatatgattt ttaaagtaga atatccggat tatccagatg taccacaaac acaaactcag
     9061 gaaaatcagc tagtcgtaca gtaataggct agctgagaat aatcctgaaa tattagtaga
     9121 aaggagagtg aagataattc aagcaatcgt acaaaaattt gtcaacaaaa tgagtgtcgg
     9181 tttcttgctt ttgttgctga cgctagtctt ttttgtttgg acagcagggg taggttttcc
     9241 agcaactttg tttatatata acaatagttc attacttatc gcttttagtg tggctatttt
     9301 gctcatttta aatgtttata aagcaaaagg cgtcgatttt ctttatctag gtgttgcgct
     9361 tgcccttttc attttctttt cttactttcg ggaattaaga aatgagtctt tatttggaga
     9421 cttactgttt cctttggtta ttttattttg tttagtaatt aaatggatcg agtggacaaa
     9481 atgggatcgc ggtatttatc tgattgtctt tacggtcttc ctcggaatca cgttatatcg
     9541 tgtatttaca gagatccaag taccagaagg acaaagcatc tgggctccag gcaattcgct
     9601 ttctgatatt tggatcaata cgaatacaat cggaagttct ttgatgactc ttgctttatt
     9661 gatcagtgga tttgccagtt cttttgaacg atggtatgtt cgaatcatcg gtgggatagc
     9721 agtcgttgca gcctttttag gtatttgggt ctgccaatct agaggtgcat tagtcgcgtt
     9781 gattgttttt gcgatattgg atcttttacc taaatcgttg atgcgtgtca ttcgtgcgcc
     9841 atttattgcc tacacggtta cgattctttt agctttgccg atttcttatc tggcggctgt
     9901 ctctgaaaaa gtcaatttgt ttaccgatcg agaagatatt tggcataaat tctatcagac
     9961 gctaggagaa aaatctgaac aagttttact aggaatgaaa acatttatct tccagcgagg
    10021 aaaccaattt ttaggaaacc acaatagtta taattctatt ttgaatatct atggattgat
    10081 aggatttggg attgctgctt tgctgcttat cctttttatt ggtcgcttaa cgttgaaagc
    10141 agatttatcc aacgggcaga tgacttttat ctgggccttt tttgcggtga tgatgcagtc
    10201 atttatggag gatacattga cttcataccc atgggttccg attatttata tcctgttggc
    10261 tatggccgtt catcgatatg atgaaccaga agttgcggag gaattctatg ttatggaaga
    10321 ggcatatgac gatatgactt atgatgatac atcttatgag gaacaaccag atagcgctca
    10381 ttcttccagg atccaacgct atcattgata ggcacaaaat acgagagagg ctgtgacaac
    10441 cgtcgcagcc ttttttggtt tattgcaatt gtctagcatc ttctgtaaaa tagaaacgtt
    10501 agagaaaatc ttgtatcata gaaaattaag aaagaaataa gaacgcagct cggtaccaaa
    10561 aatatggtaa aatggcactt gactgtaatt cactgaaaat cgcttggagg aacacaaatg
    10621 tttaaagatt tgtatttatt tatgaaggac atttatcaga acagaaaatt attgctacag
    10681 ttttcgatca atgacttcaa atcaagatat gcaggatcgt ttttaggaat cttatgggcg
    10741 tttatcaatc cgctatttac tgtattgatt tattggctgg tatttggtgt tggactgaaa
    10801 gcacaaatga tcgatgcaaa atacccattt atcgtatatt tagttaccgg gatgattccc
    10861 tggttcttct tctcagatgc atttgcatca actacggttg tttttcggga atatacttat
    10921 ttagtaaaga aagtcgtatt caatattcgt atcttgccca cgacgaaaat cctatccaat
    10981 ctgtacaccc atttattctt cattctgatt ggcatagctg tcgcgcttat aaatggtata
    11041 tatccttcag caatgtcttt acagctgatt tattatttat tttgcatgtg tgctttcttg
    11101 acaggattga cttggctgac ggcatcgatc caaccatttt tacctgatat catgcaatta
    11161 attacgatcg tgctgcagtt gatcatgtgg acattaccga tattatggag ccctaatcag
    11221 tttgattcag gtcttgtaag catattgaaa ttaaatccgc tgtactatgt tgttcaaggt
    11281 tatcgtgagt catttttaag tgaagcttgg ttctgggagc atggaacata tactttgtat
    11341 ttctgggttt tcacgttagt aatgttcgta atcggctctg cagtgttccg tagattgaaa
    11401 ccacattttt cagatgtgct gtaaggagaa tagctatgac agaatatgca attagactaa
    11461 aagatatcac aaaatcttat aatatgtacg caaaaccttc ggatcgattt cgtgaagccc
    11521 tcaatccttt taagaaatcc tatcatgatg tattttatgc gttgaaaaac gtgaatgtag
    11581 aaataaaaaa aggtgagatg atcggtttcg tgggagaaaa cggatcggga aaatctacaa
    11641 tgttgaaaat cattacggga gtactgacac caacatccgg cacgatggaa atcgaaggaa
    11701 aaatctcagc tttgctagaa ctaggatcag gattcaatcc tgagtattct ggctatgaaa
    11761 atatctattt aaatggaatg gttctaggat tttcaagaga agaagtcgat gaaatggtcg
    11821 atgatatcat tgaatttgct gacatcggag accatattta tcaacctgta aaaacttatt
    11881 caagcgggat gttcgttcgt ctagcttttg ctgtagcaat caatgtgaat cccgatattt
    11941 tgattgtgga tgaagcattg gcagtaggcg atcttgaatt tcaactaaaa tgtatggaaa
    12001 aatttacaga aatcaagaac tcgggaaaga cgattttatt cgtttctcat gatatcagtt
    12061 cgattcgtcg tttttgtgat cgttcctatt ggttgaaaaa cggagaagtc gtcgaatatg
    12121 gcgatacgat ggaagtaacg acgaattatg aaaacttttt aaagaaaaaa tcagttaaaa
    12181 cagtggatcg gaataaaaac caagtagaac ggttcgccgc tccagatatc gtcgacgtga
    12241 cgaaagcgga attgttggat aaagacaaaa atccggtgga tatgctggaa caaggagaaa
    12301 aactatacgt taaagtaact tatgaagtaa aagatgatac aatcaaaaat ccggttttag
    12361 gaattgcgct acgaacagtc gacaattttt atgtatgcgg acttaatact ctactagatg
    12421 gtatcaagat tccttggaaa aaaggagaga atatctttta tctggagtat gaaaagttag
    12481 gtttgttagc tggtgaatat tattttgacg ttgcggtctt tgaagaaaat gcaacggttc
    12541 ctttggtata taagacaaag tacatgaact tgtttgtcag cggttcttat atcggagaag
    12601 gcatcgtggt cttagaccat aaatgggaag aagggactca tagcaatgaa atatgatttt
    12661 gaaatggacc tagatgaaca aagcagtgtg ggaaaaattg ccgcacagat caaaccagga
    12721 agtaaagttt tagagtttgg tcccggaaac ggccgattaa cgaaacattt aatcggagca
    12781 aaacaatgtg aagtgagcat tgttgagtta gataaggaat tgttcgattt tgtcagcgaa
    12841 tttgctcaag atggatttta tggtgatatc gaaagttttg agtgggcaaa ctattatgct
    12901 ggacagacat ttgattacgt cttgtttgcg gatgttttag aacatttggt agatcctgga
    12961 aaaacattga agaaagtcag agaatttttg aatgaagaag gagagatcct gattacattc
    13021 cctaacttgg cgcataattc tgtcatgatc gatttgttca ataaccaatt gccgtgggct
    13081 tcttatggtc tcctagatga aacccataat tctttttata ctcatgatgg cttccaaaaa
    13141 gtttttgaaa aagcgggatt attcattaat attgaagatt atttgtactt agctgtaggt
    13201 gatacagaat taaaatcaac ttatgaagaa ttgccggaag ctgtacgcta tgatttcaaa
    13261 atgcgaccat ttggtgaagt ctaccaatac tttttctcat tgatgaaaca tccagtagca
    13321 caaagttcca ttgcagagcc acaaaattct aattatgtaa aagtgttaga agtaacccag
    13381 caaacgaaac aagatgaaac aatccaacaa ataccattca ataatttcac aggggaaaat
    13441 gagacactaa gtttccccgt atcagaaaca acagaaagaa tggtgtttcg cttcgcgcaa
    13501 caacctagct ttatcgaatt cagcgcagaa gctgccggag aaaaactaac atttatcgat
    13561 tcgaatgctg tagtcaaaac agtaaatgat tgttatttgt ttgatgggaa agaattacca
    13621 gagttcgttc taacggatat ttctgggaaa gaagtgacta tccattgtca ctatcgcttt
    13681 atcggggaat tgacaccaac aatgaaagaa ttgttggaaa cgattcgtcc catggctgaa
    13741 gtcacaaagc agctttctga gaaaaatgac gaattagaaa gagaaaatca tcatcttctt
    13801 gaggaaaata caagactaga ccatacgtta aaaacaacca caaatcgcta ttgcacattg
    13861 ttggaaagtg atgaatggac aataaaacat agactaggac gacgtaagaa agaaacttcg
    13921 aaaaaaatcc aagaaaaaga actttctata tgtattgacg agaaaatctg ggatgcagaa
    13981 acaaaaatat taaaaatcat cggatgggga atcgctaatt cggatcgcca gcccttgtcc
    14041 tataagttat cagctgacca atcgccattt ttcgaagcaa ttcctgtttc acgagaagaa
    14101 gtcaatcaag cagaacagct cgcaaaagga acagaagcgg gatttgagtt acgaatcctt
    14161 tgcgagcaag aacgttcatt cttagtggaa gcagtagctc aaaatggcca atcatggatc
    14221 attgaaatgt aaataatgat taacttataa agataaggag taaacgaatc gtgcaaaaaa
    14281 aagaaattgc cgtatttatc gatcaaatca ctcgtgaacg ggccactggc gatttgctta
    14341 ttgtgggctg ggcaattgat gaagtgacca aagaaatccc tacgataaag gtagaaaaag
    14401 aaaatgtgat tgctgaagca acacatgtcg taagattgga tatcaatcat ttatataatc
    14461 ttgatgttaa gacccaaagc ggtttcaagg tacgtttatc agggaaaatg agaggaaaag
    14521 ccatcttaga cttccaaaca gcgagacatc aaaatggcat agctgtgaaa ttaaacggta
    14581 aataccctta tgatgacggc attgaatctt catgggaaag aaaaaaaaga ttactgaaaa
    14641 aagggataaa ttatgcaagg actcatggcg taaaaaaagc gatccgtcgg gtcaaattgg
    14701 aattgaagcc aggatcgatc gactatgcag agtggatcag tcgtcatgag aaaccagatt
    14761 tgaaagagca aaggaaagaa gcacaaggat ttgcgtaccg tccattgatt tccatactta
    14821 tgcctgtcta taacgttgaa atcaaatggc tcgaaaaatg cattgattcg gtactggatc
    14881 aaacgtatga tcattgggaa ctatgtatca gtgatgatgc ttctactgat ccagctatcc
    14941 gaaagtgtct ggaatcctat caagcaaaag atgatagaat caaagtcgtt ttccgaaaag
    15001 aaaatggaca catcagtctg gcaactaatt ctgcattgga aatagcagaa ggagaattta
    15061 tagcactctt agataatgat gatgaattac cgccatttgc attatatgaa gtggcaaaag
    15121 tgctaaacgt acatccagaa ctggatgtga tctacagtga cgaagataaa atcgatgcag
    15181 atggcaatcg ctttgatccg catttcaaag cagactggtc gcctgataca ttgatgggaa
    15241 ataattatat ctctcactta ggcgtatatc gaacaagcat tgttaaagaa cttggcggtt
    15301 tccgtaaagg ctatgaaggt tctcaagatt atgatttggt ccttcgtgtg acagaacaaa
    15361 tccctgcaga tcatatttac cacattgacc gagtcttgta tcattggcgg acgatcccag
    15421 gatcaactgc aagtaatggc gaagccaaaa gttatatcta tgattccgga gtgaaagcat
    15481 tgacagatgc cctgtctaga agaaacatca aaggaagcgt acgtcctggc agaatttctg
    15541 gattttatga gattgcttat gacgtcttgc aagaggattt ggtcagtgtg atcattccaa
    15601 cgaagaatgg ctatgaagat ctaaaaactt gtgtggattc gatcatcgaa aaaacgtctt
    15661 attcaaatta tgaaatcatt attgcggata acggaagtac agatccaaaa atgcaagaat
    15721 tatttgcaga atacaagcat cagctgaaag accgctttat cgtagaattg attgacattc
    15781 cattcaatta ttctcgaatt aacaatttag cagcagaaaa agcaaacggc aaatactttt
    15841 tattcttgaa taacgataca gaagtgattg agccagattg gatgacggtg atggtttctt
    15901 atgcacagtt tgatcgaatc ggctgtgtag gagcaaaact gttttatcca gatgatacaa
    15961 cgcagcatgc aggagtatta ttaggaatcg gtggtgttgc agggcatgca ttgaataact
    16021 atgatcgaac acattgtggt tatttcggcc gattggtcat tgacgtcaat tacttagcag
    16081 tcactgcagc ttgtatgatg gtcaaggcag ctgacttcaa tgctgttaac gggtttgatg
    16141 aaacattaga agtagcattc aacgatgttg acttgtgctt gaaagtttat gaactgggtc
    16201 gttacaatgt ctatgcacat caagcagaat tgtaccattt cgaatctaaa tcaagaggtt
    16261 atgaagatac tccagaaaaa caaagacgtt ttgctggtga aatcaaaaaa atgcaagata
    16321 aatggccagc atatattgcg catgatccat tttacaatga taatttaaca aaagacggaa
    16381 tcggcgactt ttcgttgaga ccagattaaa ctataataaa aagctgttat gttgtgattc
    16441 gctcgcacat aacggctttt tatagttttt tgtcaaaaaa aggcagaagg tgccgactca
    16501 aaaaaataag tcgaggttgg gacagaagtg tttaactccg agaaataaga agaaattcac
    16561 gaaaattgct tttcaaattt ttgtgaattt cggcttattt aagaagaagt tgcttctgct
    16621 cccaccgttt attcgttttt aaagaatgag acaaaagtgt ttcttacttt tgtcccacgc
    16681 tcttattcat gctcagagcg ccaatagata caaaatcata gccaaagttt cacgaaccct
    16741 agctgtcaaa gaatcgatat tttattttaa tataggctat tttaaaataa atggtttaag
    16801 gtattcctct acaaaagcca ctctatcaat tatcatgggt ttaaaataag aagtgatgga
    16861 gataaccaga ttgttcttag cgtcaatata aagaacattt ccactattac caattgctga
    16921 atatatttcg ttctcagaat ctaataccca ccataaatag ccatatctca tattttgtaa
    16981 gtcttctaaa gcactggttc taggtttgaa catctcgttt atccacatgg gtggtacgat
    17041 ttgttttcct ttatacatcc ctttatttag gcataataca ccaatttttg ccagatcact
    17101 tgccgataaa cacaaaccat atcctgccgt cccgatattt agtggatcac aaaaccagac
    17161 attggtttta ggagacttat ccattgtaaa ctgcttatgt tcttcagctg tttcagcata
    17221 ataattgaca tggtttggta tccctatagg atcaaataga tacttattgg caaaatcaac
    17281 agttgttagc ttacttgtct gatacaacaa gccagttaat atgtgtaaac agacagtttg
    17341 atagttgaat tcgccggtta taccttttct tcctcccaat aaatctagtg atgtttttgt
    17401 ccaatcttca cttgaacaga cttttgtcca tggatcgcct ttacatttgt agggtgcttt
    17461 cattgttagc aagtgcttga ttgtaatagc gtaaatagtt ttctccccgc gtttcacttt
    17521 atactctgga aaataatcta atactttatc atcgatactt ttgattaatc cttgctctaa
    17581 agcaatgcca atcagcaaag atacaatact tttcgttgct gacatgatat gacaagtatc
    17641 gtctttttta taaccattcc attcatctgt ataaacttct accccatcct tatatgcgac
    17701 aatttgacat atatttggtt gtcgaaaacc aataaaatca tgcaactctt gtttattcat
    17761 aagatattac ctcactttct tctataagca taatgtcatt aaaaatcgat ttcaattttt
    17821 tcgattcttc acgtaataca ggtattctta tggactagac agcgctttgg gaagatggat
    17881 tctctgaaaa atataaaggt tcactttatg agtggataaa aaaagcagca aatacttcga
    17941 cttctttaat ttaccaacat tagtgaaaat cgattcatct ggagatttgt aatttagagt
    18001 ccttgtcttg attagaggtg tataaagctt tttattagac atttttaaat tccattacaa
    18061 tttgcttata aactaggttt ttaaaggtga ctatggtaag ataatagaga tattattttg
    18121 tgattaggag tgtatatatg aataaaaatg gagaatggag tgctgcacga cggatattca
    18181 ttatattaat ggatgttatt gtatacaatg cagcaatata tagtagtttc ttactgaagt
    18241 ttcgtggaga gatcccatta cgaaatttag aaactttcca acactcagca attattattt
    18301 cagtgatttt tattgcgttg aacattttgt tgggtgcata tgttttttat aatcgaatga
    18361 tcagtgacat cgtgtttgtc acagtcatcg gtcaggtatt gatgacacta gggatcatgg
    18421 tagtgacttt tgcgggccgc tggtttgctt ttccacgtct tgttatctta ctttcatttg
    18481 ttctcggtac gcttttctta agtatctatc gtattctcgt atataaactt tatttacgcg
    18541 taagtcgaga taaaaaggtc gtcattattg ggttagaaaa ggatgtcgca ccagcaatcc
    18601 aaaattttgg ttcaaagaaa aacaacaaac ataaagtcga agcagtcatt cttgataact
    18661 attatgcaaa catcaaaaat atgattaaag acatcgatat tgtctatctt gcttctcata
    18721 tcgaagaaac tgaaaaaatc aaaatctatc aattagttac taaaaatgaa aaaaaattgt
    18781 ttttgaatac aacatttgaa aacttgacat tgatcaatcc aaatattatg aatttcgaag
    18841 atgaaagtat tattgaagca tctggattta gaatccaacc tgaatatgaa ctattcaaac
    18901 gtttgtttga tatcatcgtt tcacttgttt tattagttat tgcttcacca tttatgttat
    18961 tcacagcaat tttagtcaaa gtgacatcac cgggaccaat catctataaa caagtgcgga
    19021 tcacaaaaaa tcaaaaggaa ttttcaatct ataaattccg ctcgatgacc gccacagccg
    19081 aagcaaaatc tggaccagtt ttagctaaga gcaatgatgc acgagtaacg cctgtaggta
    19141 aatttattcg agctgtccgc tttgatgaat taccacaaat cttcaatgtg ctgaaaggcg
    19201 atatgtcgat cgtaggacca cgtcctgaac gttctttctt cgtcgatcag ttcaatgcag
    19261 aaaaccctta ttactactta cggcataatg ttcgtgctgg tatcactggg tatgcacaag
    19321 tctatggaaa atatgtctct gattacaaca gtaaattacg ttttgattta ctttatatca
    19381 aaaaatattc attgatgctt gatttaaaga tcttgctgca aaccatcaag atcctatttg
    19441 ataaaatgtc ctctcaagga cttgaagaag acgaaaatgt gatctcattg gcagaaattg
    19501 cttttccgaa tgaaaaaatc tttcgctagt tgggaggcgt aaaaatgaac aatccgaaat
    19561 tcagcataat cgtaccagta tacaatgcag aagcaactat tggtcgaacg attgatattc
    19621 taaaagaatt ggattatgaa gagtacgaag tcgttttagt aaatgatgga tcaacagatc
    19681 aaacacagga aatcattcaa gaactgattg aaaatgatga acgttttgaa ttgattacta
    19741 cgaccaatca agggcccgga ttagcaagaa acgaaggaat aagacagtca aaaggagaat
    19801 atctcttatt cttcgatgca gatgacacgc cagtaaaaac gatcttgtca gattatgatc
    19861 atttgctaga aaagagtcca aaagcggatc ttgttgttag ttcgtttgtt tttcgtacaa
    19921 tggacgaggg aagaatcgtt tccgaaaaag aaaacttagt tacagaatgt cactatctaa
    19981 ctcacgaaga gtttataaaa gacatgtatc agttaatgaa tgaacagttg atgtatgttg
    20041 tatggaacaa atgctatcgt agcgatattc tgaaaaataa ccaaattcta tttaaaactt
    20101 ataatagttg tgaggatcga atatttaact tacattatta tgaatactgt catcaagtga
    20161 tcatgaaccc aaaagtagaa tatatttatg aatttgaagg tgggaagggg atcactaatc
    20221 aataccgacc taacaagttc gaaacattta aagaattcta ttcattagca aatactgtaa
    20281 cagacgatat taataaagac ggtatggcgg cgcttctttt aaaagggaca acttcagtta
    20341 ttttttctat ttatagtacc tctgcaagaa cttcaaaaga gaagaaagac gaagcaaagc
    20401 agatattgag tgatccagtg atccgagaag ccaagcaaat tgctaagacg gattcttcag
    20461 caaaaaaaat aacaaagttg ctttacaata tgccagctcc actattttta tctgctgtaa
    20521 aagttggaag cttcgttgaa gtgaaaatgc cagggttaat ggctgctttg aagcgagtat
    20581 attagttaat ggaggaaaat ataatgaaaa gaattttgca ttttcaggga cgaatgggtt
    20641 taggtggagc agagtctttc atgatgaatt tatatcgtaa aattgatcgt acaaactatc
    20701 aatttgattt tttgatttat gaagattatg cagatgttca agactatcat tcagagattg
    20761 aaagat
//
LOCUS       DARDHA010000034        20412 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000034 DARDHA010000000
VERSION     DARDHA010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.034"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            18..1097
                     /locus_tag="SDA10_001031"
     CDS             18..1097
                     /locus_tag="SDA10_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG4239959.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
     gene            complement(1294..1995)
                     /locus_tag="SDA10_001032"
                     /pseudo
     CDS             complement(1294..1995)
                     /locus_tag="SDA10_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(2009..2933)
                     /locus_tag="SDA10_001033"
                     /pseudo
     CDS             complement(2009..2933)
                     /locus_tag="SDA10_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            3050..4129
                     /locus_tag="SDA10_001034"
     CDS             3050..4129
                     /locus_tag="SDA10_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG4239960.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            4161..4754
                     /locus_tag="SDA10_001035"
     CDS             4161..4754
                     /locus_tag="SDA10_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4239961.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(4864..5091)
                     /locus_tag="SDA10_001036"
     CDS             complement(4864..5091)
                     /locus_tag="SDA10_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG4239962.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(5194..7782)
                     /locus_tag="SDA10_001037"
     CDS             complement(5194..7782)
                     /locus_tag="SDA10_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEG4239963.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(7802..8461)
                     /locus_tag="SDA10_001038"
     CDS             complement(7802..8461)
                     /locus_tag="SDA10_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG4239964.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(8510..9013)
                     /gene="trmL"
                     /locus_tag="SDA10_001039"
     CDS             complement(8510..9013)
                     /gene="trmL"
                     /locus_tag="SDA10_001039"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEG4239965.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(9234..10100)
                     /locus_tag="SDA10_001040"
     CDS             complement(9234..10100)
                     /locus_tag="SDA10_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG4239966.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            10209..10850
                     /locus_tag="SDA10_001041"
     CDS             10209..10850
                     /locus_tag="SDA10_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEG4239967.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(10912..12273)
                     /gene="mgtE"
                     /locus_tag="SDA10_001042"
     CDS             complement(10912..12273)
                     /gene="mgtE"
                     /locus_tag="SDA10_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEG4239968.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(12291..13190)
                     /locus_tag="SDA10_001043"
     CDS             complement(12291..13190)
                     /locus_tag="SDA10_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG4239969.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(13192..13989)
                     /locus_tag="SDA10_001044"
     CDS             complement(13192..13989)
                     /locus_tag="SDA10_001044"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEG4239970.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(13967..14671)
                     /locus_tag="SDA10_001045"
     CDS             complement(13967..14671)
                     /locus_tag="SDA10_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEG4239971.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            14802..15377
                     /locus_tag="SDA10_001046"
     CDS             14802..15377
                     /locus_tag="SDA10_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEG4239972.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            15495..16172
                     /locus_tag="SDA10_001047"
     CDS             15495..16172
                     /locus_tag="SDA10_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEG4239973.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(16251..18059)
                     /gene="pepF"
                     /locus_tag="SDA10_001048"
     CDS             complement(16251..18059)
                     /gene="pepF"
                     /locus_tag="SDA10_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEG4239974.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(18235..19233)
                     /locus_tag="SDA10_001049"
     CDS             complement(18235..19233)
                     /locus_tag="SDA10_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEG4239975.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(19459..20112)
                     /locus_tag="SDA10_001050"
     CDS             complement(19459..20112)
                     /locus_tag="SDA10_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEG4239976.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5907 a         4441 c         3639 g         6425 t
ORIGIN      
        1 gacaaggatg aattcacatg acgcaaaccc atgataacac agtttcacgc aagggaaaac
       61 atctttccta ctcagaacgt tgccaaatcg ctatcctgaa aaaggaaggc tattccaacc
      121 gtcaggttgc caaagtgctg ggacgtgttc cgcaaaccat aaacaatgag attgatcgcg
      181 gaaccatcac ccagttaaaa cgccaaacgc aaaatggaaa agtgtatgac acctactatt
      241 ctgtttatga cgctgatgca ggacaggcct tctatgaaaa gcaacgactg aactgtggca
      301 gacgccccaa atgggcgaac acaaatgcct tcattgattg ggcagatgac aaaatgttaa
      361 atgaaaaatg gtcgccggac gtcgtggtcg gatttgccag aaaacacgag ctgttcgatt
      421 gtgccatcat tcctggcaca accgctcttt atcaatggat tgaccgcgga atcatgcgaa
      481 caaagaacat ggacttgctt gaaaaactgt cccgtaaaat caaagcggtc cgcccaggac
      541 accgtcccaa caagcgtgtt ctcgggacgt caattgaaga acgccctaaa gaagttgaaa
      601 cacgggaaac gttcggtcat tgggaaatcg acacggtagt gggaaacaag accaagtcgg
      661 atgccgtgct gctgacgctg gctgaacggc agacacgctt tgaagtcatc ctaaagttga
      721 acgggaagga caccgaatcg gtggaccaag cggttcaaca gctaagagaa cgggctggcg
      781 ctaacttcga tcaattattt aagaccgtta catccgacaa tggctcggag ttttctgggc
      841 tacacgatgc tctgcaagat gtagctgacg tctatttcag ccacccctat gcttcttggg
      901 agcgtgggac gagcgaaaac cagcacaaat taattcgccg tttcgtcccc aagggaagcc
      961 cgattagtga ggtgagcgag aaccagattt tgcggatcca gcgttggatg aacgattacc
     1021 cgcggaaaat tttgggttat gcgacaccac atgatacatt cgtccaggca ttcaagcaag
     1081 aacggttggt ggcttgaccc attattcctt gataacgatc ctcactcggg acatggcttc
     1141 atgtctggaa agctggcctg gggccagaat cgtctgaaga ctatcatacc tcagttcatt
     1201 atcaaggcct cactgattta gaataacgag gcagttacgg tatcaaacaa atgagttacc
     1261 tgtagtggct aacttaaact tgaaatttac ggacgtaaag cttgctgcca aattagcgat
     1321 tgaaactaac gattgctttc taggaatcaa ggacaagcga ataccatttg acgattgatt
     1381 tgcttgctga tcctgtacat tgcggacccc ccacatcgtc ccgtagagaa tcgccattcc
     1441 aatcagagtg aagaaataaa aacttttgaa actcatatta cgacctgcat gaacttcttg
     1501 aaaagatact tcttgttcaa aaacagtcat gatgggcgcg agatcaacgt tctccacttc
     1561 tgccacttta ctcgaaatta taccaatacg ctgtaaaaat tgatccatga cgtttttcat
     1621 caacgtctga ctcattcctg cttgacccac atggagcgaa acagatgaac catcaatgag
     1681 ataaaaacca gccaaatcat ctgcatttag cttttcattc gcttctactt ccgatagttc
     1741 ctgtacttca aataatggtt tatcattgac ttcgacttgt tctaagactg tggtaaaatt
     1801 ttccgaaagc gctgtttgat ccgctttgat tacaattcca gtattaatcg tttcgagatt
     1861 gccaccttga tcaatctcgc caaaggccgc cataaacatc aatcctaaaa gaattggaaa
     1921 ggccaatgtc caaaacaaca tggatttatt tcgcagcagt atttttgtgc gatatttata
     1981 aaaatgccaa aacattattt cccctcctct agtctctcaa atccttgcca gtaatctcta
     2041 aaaagacatc attcaaggac gcttctttgg ttgctatttt tccgtaactg atcttttctt
     2101 cttgtaagat tgtaagaatc ggcaacaata ctgtatttag ttcagtagtt gttacttgga
     2161 gctgaccttt tttataagcg attccatcag aagctattgt tcgctgacaa cacgttttct
     2221 gggagtaatg ggatgtcgat cgtcaaacga tccgccgtcc gtaccatatc ttttagagat
     2281 tctttggttc ctttagcaat cacctgtcct tgatccatga tcacgatctg gtcgcaaaga
     2341 atttctactt cttccatgta atgtgttgta tagacaatgg ttgctccttg tcgattcagc
     2401 tccttgatac tttctaaaat tttattccga ctttgggggt ctacagctac tgtcggctca
     2461 tctaaaaaaa ttaaccgcgg cttgtgggca atcccacatg cgatgttcaa ccgacgtttc
     2521 aagccaccac taagttctcc tggacgaaac gaaacatatt tttctaatcc cactaattga
     2581 atcacctctt caatcaaggc tttccttttc tctttttcgg aaacatataa accgcaaaaa
     2641 taatcgatat tttcttgcac acttagttcg tcaaagaccg caacgtcttg aggaacgacc
     2701 ccaatcattg ccttgacagc ataattttca ggagtcattg ttgtcccgaa caatttaatg
     2761 gttcctttgt cataatttaa caaagaaagc atacagttga tggcagtcga tttcccactg
     2821 ccgtttggtc ctaacagacc taagacttct ccttcttgta cttgcagatc aaaatgattc
     2881 aatgctacaa aatctccata gcgtttgact aatttatcaa tttcgataat catcatgaaa
     2941 aactccttcc aaatttcttg ttgatctgac tataataaag tgcaagaaaa gaaactagtg
     3001 acgattggct aaaccacaca tgacatttgt cacaatgaag gagtctgcta tgcataaatc
     3061 tcggctactc aacgaaacta gctttctact ggttatctca ttgcttttgc tggtaagtca
     3121 aggattcgtt gctatgcaag ttatctattt attgatcgcc gttttattca ctggtgtcag
     3181 ttatttactg cctacaaagc caaattggat tttccaagtt attctatttc ttttatgtgc
     3241 ctttttacct gatcttgtcc tgtttctgcc agcgacgatg cgagtgatcg cccctgaaga
     3301 aagccacctt gattacttgt ttttgttagg ttttttgttg atttcgacgg ttggtaatca
     3361 tccccccatc aaggtcgcct ttgtcgtaat tttgctgttt ttttcctttt acttacgaaa
     3421 aaaagatcaa cgaacgaccg atcttgagaa aaagtacatt gtctccaaag atactcattg
     3481 ggaaaaacaa caagagttat cggaacaaaa taaagcattg gccctgtcac aagaaacaca
     3541 attagccttg caaattgtgg aagaacggaa ccgaattgcc cgagacatcc atgacaatgt
     3601 cggtcatctg ctgtcaagtg cgattttaca aattggtgcg cttgaaatga tcaatcaaga
     3661 accaaaacta aaagaaccat tagaaaattt aagtgccacc gttcacactg ggatggatcg
     3721 gattcgtcaa agtgtccatg acctccatga gacatcgatc tcttttcaac actccttaga
     3781 gtttctgatc gctgattttc cttcttctgt tacgattgaa ggaaacttgt ttgaaggatt
     3841 gaatgaaacg cagcaaaact gcttcttaat ggtcatcaaa gaagccttaa ccaacagtat
     3901 caaacatagt catgccaaaa ccgttgtttt acattttcga acgttgccgg ccttttatcg
     3961 cttgcaaatc acgaatgatg gcaccagccc acaaaaaaat tctgctcccg gtattggttt
     4021 gaaaacgatg cgtcaacgtg ttcaagaaca aaagggacaa gtccatattt tccaagaaaa
     4081 ccagcaattt cagttaacga tcattttacc aagggaggga caccaatgat aacagtagtc
     4141 attattgaga tgatccattt gtgacgaaat cgttacaaac cattttagaa agcacgaaag
     4201 agatccgtgt gttaggcatt ggtcattgtg ccaaggatgc gctagtctta tacgaaaccc
     4261 atcgtcccga tgttttgctg acggacatcc gcatgcctaa acaaacgggc atcgatgctg
     4321 ccaaagaaat tttggctaaa tttcccaaag ccatcctttt gcttttgacg acattcaaag
     4381 atgaagaata catccgcgaa gcattttcca tcggtgtcaa agggtattta atcaaacaga
     4441 acttgcaagc gattattcct tctgtcaaat ccgcttataa tggacaggcc gtatttggta
     4501 atgaaatcat cgagactttc actcaaatga tgaagaataa accgacgatt gatagtcatg
     4561 cttatgcttc ttttagtgaa cgtgagcttg caatcatcaa agaagtagct gccgggaaaa
     4621 acaataaaga aattgcagat gccttgtact tgagtgacgg cactgttcgc aattacatca
     4681 gccaattact agaaaagcta gatttacggg atcgcacgca attggctatc tatttttatc
     4741 agcatttgca ataagtctta tctaacacaa aaaaacctga atgatgctcc ttcgcatcat
     4801 tcaggttttt ttcacatctt ctattcaatt gtcccgcaaa ccatttcctc acgattgaaa
     4861 gaatcaagga ttcgcagggc tgccaggatt cgttggattg ttcggcgtgt taggcggaaa
     4921 aataggcgcc ttgtctaacg ttttttctag ttttgattct tcacgatcgt ctcgttctac
     4981 atcaggactt tcattcaatt tgaattcttt ttccatctca tctaactttt taaagtcatt
     5041 gccagattct tttgctaatt tcgctaattt atcttcggtt tctttagcca taaagtaacg
     5101 cccccctttt gatttcagct tagcatatca cactgaaaaa acaaaagaaa acgctaacca
     5161 acgatcattt ttagctattt ctcatcttta aaatcaagca ttttttgccg gtataaaatc
     5221 gtagggagaa ctgttttcca taccaatcat cggatctact aactggttat cgatcacttc
     5281 tgctgtaaga acgaaatctt tagaaacagc aggttctacc tcttctttct tttcttctcc
     5341 aacaaatgaa gaaaacagat ctggttccac tgtttccatt acttggtttt tcgtttcatt
     5401 cgtcccactg tcatcttcta caggtagctt ttcttttaca acccgtggct cttttgtata
     5461 gttcccatct tcaaatggat catttccgac caaagaatct tcataagctg ctagtttggt
     5521 ccggagagtg atattcattt tatcattatc catgatcctt gatttcaacg tagtcatccg
     5581 gcttgctgat tcattcgcta cacaagtgac ataggcttgc tcttccccta acaaaatcaa
     5641 cagttcctta ctgcgagtca ctgccgtgta gagcaaatta cgctgaagca tccgctgata
     5701 ttgatggacc atgggcaaaa taaccatctt gaattcactg ccttgtgatt tatggattga
     5761 gcaacaataa gaaagagtga ttttattcca ttcgtttctt ttataagtga cctcatttgc
     5821 atcaaactgg ataactaatt catccacttt atcttcagaa tcttttgcca agatgatacc
     5881 cgtgatttct cccatatctc cattgaatac attcaattcg ggagtattga ctaattggag
     5941 gactttgtct cctatccgat aaacggtgtc gttccaatgt acttcctttt ttccaggttt
     6001 ccctggattg aatatctcct gcatcatttt atttaacgca tcgatccctg caggtccccg
     6061 atacattggt gctaatactt ggatatcttg tgcacggaat cccttatttt tcgctttttc
     6121 cacaatttta gagatcaagg gttcaatttg ataagcatca cttcggaaat aggaacgatc
     6181 tttttgattc tttgtgaaat cttttggcag acgtccttct ttgatctcat gggcaagagg
     6241 aataatacta gaaccatcac cttgccgata aatctgattg agttccattt tgggaatttg
     6301 ttcgatttcc aaaagatcat ggagtacttg accaggaccg acagagggaa gctgatcttt
     6361 gtctcctact aagataactt gcatattcgt cggaatcgct ttgaataatg tgttagccag
     6421 ccatgtatcg accatagaca tctcatcaac aatcaaaagt ccgccttcta attctttcgt
     6481 actgactgaa ggtgtttttt cccgtccatt cagccccaga agacgatgga tcgtactggc
     6541 aggcagacct gtcgtttcgt tcattcgttt agctgctcgg ccagttggtg ctgcaagtag
     6601 aataggaaaa atttcttggg tatattttga aggatccaat tccagtccgt tcaactctgc
     6661 aaaaagattg acgattccgt tgatcacagt agttttcccc gtacctggtc ctcctgttaa
     6721 aataaataga ggggaacgga ttgcttcagc gatagcttgt tcttgcgaag gtccatactg
     6781 tatgccgaat attttttcga tacgccgaat attcttctgc acttcttttt caggataatg
     6841 gatttctttt ttacgactga gaaggcgctg gatcgaatta ccgatccccc attcagaaaa
     6901 gtacaaactg ttttcgtaaa gattagtctc ttcttgttgt atctttcctt cttcaacaag
     6961 ttggataatt acagtagcaa cctgatcagg ctggatttca attgggcgac ttgtttctaa
     7021 aatacggatc gtttgctgaa gcaatgtctc cgccggtacg tatgtatctc ctgtttgaac
     7081 tgcatgctga aaaatctgat gcaagatagc tgctcgaatt cttctcggtg aatctgctgc
     7141 aataccgatt tgttcggcta gattatctgc acgtttgaac ccgattcctt ctatgtcttc
     7201 gactagctgg tacggatttt cttcgatgat atccaaagac tcgttcttat aggcctggta
     7261 gatactgaaa gctaactgac taccgaaccc gtaacgattc agtcccatga tcacttgatc
     7321 catcccgtgg tttagccgaa tcgtctcagc aagcatttct ctttttggct tcgtcaaacc
     7381 ggagatttgt tttaataccg aaggatcagc aatgattttt tcgatagcat cttctcccag
     7441 caattctaca attttttctg ctgttttctt cccgattcct gggaattttt cgctggaaag
     7501 atacgctacg atcccatttg cagttgttgg cttttcttgc tcatatcttt cgagtttaag
     7561 ctgccttccg tatttaggat gatccactaa ttctccataa aaacgataag tctcattttc
     7621 ttgaatatcg ccgaaacttc cggtgactac aatctctttt tcacggtaat cagctgtggt
     7681 ttcactgatg tgtaccaaaa gcactttgta aaaattacta ggattttgaa aaaaaatcgc
     7741 ggcaactgta ccaacgaaat aaggcatttc ttctggctgc atcggaagcc gcctccttct
     7801 tctatagtaa aggttctggt gctgtttcag ttcctaaaaa atctgtttgg ttccatactt
     7861 tcagttcata tggcaataat ctgcaaggct cttcagtctc taaaatcgtc aaactactat
     7921 tgaacaatcc acccatttca cgcaactcac ttagctcttt tcctgtcatc cattgaatgg
     7981 ctgcagttaa agaagctcca tgtcctacga ataaaagtgg gccatcttta tgttgggcaa
     8041 ccgcatctgc tacaacagtt tcgatacgtg tgattgccga ttcaatcgtt tctccgtgaa
     8101 aaggagaagg atcatacaga tctaaatgat tacgcaaatt cggcagatta tccggatatt
     8161 tctggtacat ttcatcgatc gactgacctt ctaaagtacc cagccctagt tctcttagct
     8221 tatctgtata aaaaatctct accggacgag caagctcttt gttgatacct tctgctgtat
     8281 cacgtgctcg ctttgctgta ctggaataaa ttgctttaaa aggaacatat tttacctttt
     8341 tcccaaatgc tttgatttct tcgtaacttt gtggcaaaag cggagaatcg ccattgctcc
     8401 cttgaaaacg tctttcctga ttccattgtg tcttcccatg tcgggtaaaa tatagttcca
     8461 tacgcagcaa actcctgtct ttcttttatt ttactctata ataaactgtc taatccagct
     8521 tgtcattttc atagtgatgc gtcaattcta aattagggaa tccttgctgg cgaagtgctt
     8581 catacactat cattgcagct gtattcgata aattcaatga gcggacatgc tcatcattca
     8641 tagggatgcg taaacacttt tcttcgtttt cacgcataaa tgtttctggc aaaccggttg
     8701 tttcttttcc aaataaaaag aagtgctctt ctccatcagc aaaattttcg tcactatata
     8761 ctttatgggc aaattttgag atcaaatgta gtttacgatt acctaaaaat gttaaaaaag
     8821 cttctaaatt actgtggtaa gtaatattta catcattcca ataatccaac cctgcccgtt
     8881 ttagatgttt gtcatcagtt gaaaaaccta gtggctcgat taaatgaagc ggagtattcg
     8941 tggctgcaca cgtacgggca atatttccag tgtttgcagg gattaatggt tcaaataaaa
     9001 cgatatgatt catcaggatc attcctctca tatttatgtt cgtttctatt ttctgacaat
     9061 ttgatttttg cataaaaaaa aacgtatcaa ctcggtgtca gtcactgcga gtcgttacgt
     9121 tagtgaagct cttctcacta acttttatca gaacaggaac ctgataaaaa ccaatgaaaa
     9181 aacaacttgt tgctaatata acaccctcaa cttttttttg caacctctac tcattaaaaa
     9241 ctgctagaaa aacgttttct cttagttcct tttaataatt ctatgtcaat tgtaatcttc
     9301 ttcaaaggat tcttatataa gtctgctttg attcgctctt ctacctgcca ttctaaaggt
     9361 gacatggcca aaagatcttt ttgattttcc tctggtatct caaattcata ggttacacgc
     9421 tgacgttcag ttaatttcat tgcttcagca aacttggcta ctacacgttc attggaataa
     9481 ctctgttttt caggttgatt cggataaaaa gcctgacgta gttcctttaa ataattttct
     9541 tgtggcacca ccttcaacac ttctccatct tctgagagta cacgttggaa ttcttggtaa
     9601 tgagaaggcg aaaagatatt taaaaccgcg ttgaatgttt gatcagcaaa cggcaaattg
     9661 gtcaaatcag ctacacacca aaatgcttcg gcagattgtt ctgtagctgc gtagactcct
     9721 tcttttgaaa tatcaaatcc aacagcattt tgaatctttt tcaattttga aagttcattc
     9781 aagaagctac cttctccgca gccaacatct aatagcgaga cttcttcagg taaatgctgc
     9841 gcaatagtct caataacggg ctgatacatg ccagattgga tcattctacg ccttggaacg
     9901 aacattcctt gatcgtattc tgttttaacc gcatgggata aaaaataaag tgttcctttt
     9961 ttggaaaggt caaaacggtg attatttttg catatcaagc cattatcaga cttttttact
    10021 tcttcttggc aaaccggaca gcggaacagc tgatgatttt ctgccaaaaa ctggcgtcct
    10081 cggtcaattt tttttaacat agttccccct catttctgtt ttatcttacc gttagatgct
    10141 cactttggca actgctaaaa atatgataca atgcctagaa gaaagtgaaa gataaaggag
    10201 aagattagat gatcaaagaa ttttgcgctg aaaactacac atctattcct ctagcaattg
    10261 ctagaggagc cgatcggatc gaattgtgtg acaacttagc ggtcggtggc acaaccccga
    10321 gtactggtgt gatcgaagaa acgttaagtt atgctgggga aaaagatatc cccgtaatga
    10381 cgattattcg tcctagaggg ggcgacttcg tctataacga tatcgaattg aaaatcatgc
    10441 atactgattt gattgaagca aagaaattag ggacggatgg cgtagtattt ggttgtctta
    10501 ctccttcagg ctggctagac gaagaggcct tagaactatt gatcgaaaca gcagaagggt
    10561 tgcagatcac cttccatatg gcttttgaca gtattcctga agatcggcaa ttcgaagcaa
    10621 tcgattggct ggtagaacat ggtgttgacc gtatcttgac tcatggcggt cctgctgaca
    10681 aaactattgt agaaaatttc ccacggctta aagaattgat tgcctataca gatggccgct
    10741 taatcatttt gcctggtggt ggtgtcactg cagagaatgc agcacatgta gctaaagaat
    10801 tgaacgtaag cgaagtacac ggaaccaaaa tcgtggatct tcagttataa tcttataaac
    10861 aaaaaaacat ttgcaacagg cataatgctt attgcaaatg tttttgtttt tttacataaa
    10921 atagcccata aataaagaag caatattgaa ataaatcagt acactcgtca aatcgctcaa
    10981 agtcgtgata aagggaccgc ttgctactgc tggatcaaat cctagacgat ccattagcat
    11041 cgggattaaa ctacctgcaa gattcgccac tgtgattgca aatagcatag ccatccctat
    11101 gacaaacccc aagataaaat tatgtttcca aaaacctacg attagaaaaa tcgtgatccc
    11161 cgtaacagct cctgtgacaa cgccggtcaa gacttcagcc aaaacaagtc gaacaaatcc
    11221 atgatctttc tcatcagaaa tcgcaagtcg acgaacagcc acagctagcg actgggttcc
    11281 agcattaccg gcagttcctg taatcagcga aatgaagact gctaaaatac tcgcttcact
    11341 taccaagtct tcatagtgac tgattaaaga agcggtcgac atacccaaaa acaacagagt
    11401 gatcagccaa ggcaaacgtc gagacgctgc ctttactgga ttttcattaa tttcttccac
    11461 attgaccccg gcaagtcctg aataatcgct agcagcttca tcatcaataa cgtcgatgat
    11521 atcatcgact gtgacgattc ctaacaaatg atcatcataa tcggtaacag gaagagctaa
    11581 gaagtcataa tctctaaatg tttgtgccac atcttcttgg tcgtcgccta catgaactga
    11641 aagcactcgt tcgctcatca gatcagaaat cattgcgtcg tcatcattca cgattaggtc
    11701 gcgcagagaa ataaccccta ctagctgacc ttcttgatta acgacataga tgtaatagat
    11761 tgtttcagct acatctgctt cgttcttcag cacgtacatc gcagagcgaa cagtttgatt
    11821 ggctacgatc gaaacaaatt cagttgtcat gatcgcgcca gccgtttcat cctcgtaatg
    11881 gagaagttcc ttgatttcac tggcatcgtc tgccgacatc aaacttaaat atttagcaat
    11941 ttgcttttta tccagcgtat taagcaagtc aacagcatta tctgtataca tctcagaaag
    12001 catatcagct gcatagctag gacgcatttc atctaggtat tctttcatat gttcgttgtc
    12061 ttcttcaatc acatcgaaca tatccgctaa ttctttaggc gacagatagg aatagatcag
    12121 ctgcctgttc tcttctgtaa gagattggta gaattgcccc tgctcataga tatgcatctc
    12181 taaaaaactg tttctgaatg cctgaatatt attttccctt aaatcttgag tcaactgggc
    12241 aaatctttcc tctaattctt gattttcatc caaaccaatc tcacctcgcc ttattcttca
    12301 aaccgtctca caactgcctc catgtctgcg gctaaaggct gcttgaactc cagcaactct
    12361 tctgtaaatg gatggtaaaa acgcagatca taacaatgaa gggcttgtct ttttattccc
    12421 agatccattc gcccgccata catatcatca ccgagcaggg cacagccaat tgcggaaaaa
    12481 tgaactctta tttgatgtgt ccttcctgta tgcagctgga tatcaactaa cgcgatttct
    12541 gttagtcgct tctctatcca gtactcagtc tgtgccgaac gtccttcatc agagaccatt
    12601 cgcttcaata gcgaagaata atctcgggcg atcggcaaat cgatatgtcc atgtgccgat
    12661 aaatctgaaa gccttccact gactaaggcc tgatattttt tcacaaactt cttttcacga
    12721 agttgcatat ctaattttgc gtgcgcaaat ccatgctttg caaaaagcat caaaccagat
    12781 gtatcccgat ctagtcgcgt caccacatgg attacttggt tttcgtaacc ttgtttttta
    12841 taataagcct ttacacgatt agccattgtc ccatttggat ggtattgggc aggaatagac
    12901 gaaacgcctg ctggtttatt tactaccaga acgtgttcat cttcatacac gatatcgatc
    12961 ggtgtttcat ccaaaagtac ggtttcatgt tctccttcag caggaatcac aatggttacc
    13021 ttatcatcct tagctagaga aaacaagaca ttttctactt gatcattaac tttgatttga
    13081 ccgccttgaa attttatttt cgctaacaga ccctttgaga tccctttttc ttttaaaaaa
    13141 tattttactt gctgagaatg atctttctca taatcccatg taaactgcat atcaatcctc
    13201 actaataaag gcatctttca cacgatgcca aaaatgcata tgtcgataag aagcaaaatg
    13261 gatccgttct tctgctattc gataataaat tgagcggata tttgcttttg cgacattcaa
    13321 ttgatcaacc gttacaagat aatcttctgt attttccaat tttatttcta gccattcatc
    13381 atgggcaatc acgatcggag aacccaaagt acgaaacact cgattattca gtgaagcaat
    13441 ctctgccaac tgaaaggcat tgatactagg atggatcaca gcaccgccga cacttttatt
    13501 atatgctgtc gaaccagtag gcgtagaaat cgtcaatcca tctcctctaa aactttcaaa
    13561 cagctcgttt ttgatgtata catcggctac catcgtacga ttggcccgtt taatcgttga
    13621 ttcattcagt gctaaaaaat gctggtccgg tgtttcatcc aaataactga tccgaacatc
    13681 cagcaaggga taactaacac tttgttcccg gttcgttcgt aagctgtcta ctagttcttc
    13741 cagctcgtaa tcccgccaat ccgtgtaaaa ccctaaatga cctgtatgca cgcccaagaa
    13801 acgcacatca ttcagacaat gactgtagtg atggaacgcc gacaataatg tgccgtctcc
    13861 gccgactgag atgaccaatt ctggctcatg atcatctcgt tgaatccctg cttgttctaa
    13921 aagtgcgttc aaacgttctg tcacttctat agtctttgct tctttattat gaacgatcgc
    13981 tactttcatc ccctgcctcc tctctttcgc tttctttctt caaaagcgtt cgatagtaga
    14041 catcatctga ttttcctctt ccatgggaga ataaccgctg tgcttcttgg atttcttcac
    14101 ggatactgga catctcttca tccaactgat aggcagcttc tgccgcacgc tgaagtcgtt
    14161 cactcatttc ttctggaaaa acgccttgat atttataatt cagcgaatgc tcaatcgttg
    14221 cccagaaatt catcgcaaga gtccgaatct ggatttccgc caaaattttc ttctcaccgc
    14281 tgatcagctg tacaggatac tcgatcacca gatgataaga acggtagcca cttgcttttt
    14341 tatttgtgat gtaatctcgt tcttggagaa ctttcatgtc ttttctttta cggatgatct
    14401 caacaacttg atggatatct tctacaaact gacacatgat ccttaacccg gcaatatcag
    14461 acatttcttc ttctaaacgt cccattggaa tatgccgtaa ttgtgatttt gttacaatac
    14521 tatcgactgg cttgactctc ccagtgacaa attcaatcgg gacatgtcga ttttgttcac
    14581 ggaactgttt gcggatcccc cgcagtttca ctttcaattc tgatacagtt tgttcatatg
    14641 gtgctaaaaa tgcttcccaa tctcgttcca tagctgatac ctctacttca ttaacattca
    14701 ttgtttattc taccatatat gcaaagtttt ttcgtactct tgatttatgc attgaccaaa
    14761 aaaagaaaat taggcaaaat agacaaaaag gagtcgatca aatgacacaa atgcttgaaa
    14821 tcgaatataa atctatgctg acaaagcaag agtacgagat attcatcgct cactatcaat
    14881 taacagataa agatttccgt gtacagacta atatttattt cgataccgtt gatgaacagc
    14941 tgaagaaaca aaattgtggc ctacggatcc gtttcgttgg tcatcaagct gaatatacat
    15001 taaaaacacc tgctgaaaaa gggcgtctcg aaacaacgga tacctttata acagaagaag
    15061 cagaagcatt tatcttcgaa aaacgtctgc ctcgttcagg cgcagttttt caaaaacttt
    15121 ctgatttgaa tatcgatcct gcttcattaa tccaaaccgg aaagcttaca acaaaacggg
    15181 ctgaattccc tattgaagaa ggacttttag ctattgatga aagctgggga gacaatctac
    15241 acgattttga actggaacta gaggtagggg atgcatctgt gggaaaaatt gcttttataa
    15301 attttttaaa acgtttttct ttaccttatc gtcctgctaa gaacaagatc caacgtatgc
    15361 ttgaagctaa aaattagaaa cctttcattt tcacaaggca cttgagttca tttttttact
    15421 tttttctgtt aagttatagg atgtgaaaga atctagtcac tagttgtata gaatctattt
    15481 tgggggagat tcatatgatc gaaatctatt tatttgttaa tcctttaggg gaatactgtt
    15541 tcgaaatgga acaacagtta ttacaattta tcgaagaaga gtacgggaca ccatcaaaag
    15601 aaaagatgca atttcgcttt cttcctttgg tcaatttgca gacaattggg gatgtcatgc
    15661 aacaaaaagg aatttctcag aacaatcttg aggaacgtaa tcacttattc tcaatgactt
    15721 attctgcagc attggactgc aaagcggcgc agtttcaagg aaagaaaaaa ggccgccgtt
    15781 ttttgatcaa attgcaagaa gcagtcggct gtaatgacat accatattct caagaacttg
    15841 ctgaatccat atttgaagaa atcggcggcg atttgaatat gtttaaagaa gatcgtcagt
    15901 ctgacttcgt taaagaagta ttcttttctg atcaaaaaat tgctcgggaa atgggcatca
    15961 caaaacaccc ttctgcagtc gtttacaatt atgcctgcga ccgtgatttt ggtgttttag
    16021 tagaagtaaa aggtacaagt agcctttgga gaatcacaga gctgtgtaaa acaacagata
    16081 actcatatca agtttttaat ggtgaacatt tacatacaaa tgattctcag ttccattcaa
    16141 gaactagcca tttacacttg ctttctaatt aaaaaaatac gtcgactccc ttacaagaga
    16201 gccgacgtat ttttttcgtt tctattcgag aaaatcaaaa atagaaagcc ttataaacga
    16261 tcgatcaatt cttctaattc atttagacgt tgttcaaaca tcgacattgc atcttcaata
    16321 tatgcagcct gtgtcatatc aactcctgct tttttcatta cttccacagg ataatcgctg
    16381 ttacctgctt ttaggtaagc caagtagttc tctagtgcct ctggttcctg gtttagtatt
    16441 ttcttcgcca gtgctgaagc agcagaaaag ccagtcgagt attggaaaac atagtaatta
    16501 taataaaaat gcggtatccg tgaccattcg aatttaattt ctgggtcttc ttcgactgct
    16561 ggaccataat attttgcatt caatttacca taactatcac ttaggtattc actagtcaat
    16621 ggcacacctt tttcatcttc cgtatgcatg aaatgctcga attcagcgaa ttgtgtctgg
    16681 cggaaaactg ttcctttaaa cccatccaag taatggttga gtacataagc ccgtacacga
    16741 gggtcttttt ctgtttccaa taaatactcc gttaggatat tttcattcgt tgtcgaagca
    16801 atttctgcta aaaagatgga gtagtcgccg tacacataag gctggttcga acgagtgaaa
    16861 taactatgaa cactatgtcc catttcgtgg acaagcgtaa atagctgatc cagcgtatca
    16921 tgccaattca ataaaatata tggatttgtg tcatagcttc ccgaagaata agcaccgctt
    16981 cgtttccctt tattttcgac aacatcgatc caacgttcag agaatgcttt ttctacgatg
    17041 gccatgtatt cttcacccat tggtttcagt gcttctaaag ctttttcttt tgcttcttcg
    17101 tacgtaaagg taattggagc ttcccctaag accggtgtat aaagatcata catgtgcagt
    17161 ttttccactt ctaataaacg tttccgtaat tccatgtatc gatgtaacaa aggcaaatgc
    17221 ttgtttacca cgtctaccaa agtatcgtat acactttcag gaatatgatt attgctcaaa
    17281 gacgcttctc tggcagagct gtaattacgg actttcgctt taaaattatg tccttttata
    17341 tgtgtgccta aagtagaagc aaatgtattt ctaaattgtt cgtaaacact gtacaatccc
    17401 ttaaatgctg cttcgcgcac ccttcgatcc gtgctttcta gcaactgacc atacacgcca
    17461 tgagataatt ggactatttc accattttct ccttcaatcg ttggaaaaac tagatctgca
    17521 ttattcaaaa cagcaaatgt atcacttgaa gcatcaaaga tttcacctgc tccagcaaga
    17581 agagattcct gctcagcaga taagacatgg gctcgattat ctactatttg ctggatataa
    17641 tggcgataga cttccaattt tggttcttct ttgaaatatt gccaaatctg gtcatctgac
    17701 aattgcaata tttctggttc aaaccaggaa acggcttcac taactttcga aaacagactg
    17761 cttgctcttg cataaagcgc ttggtaatct gtatttccag tgtcttgatc gtttttaagg
    17821 tgcgcatata cataaatgac ttcagtttgg cgataaaccc tcaatacgaa ttcaatcgca
    17881 tttaaaaatt gagaagcgcc ttgatcaagt gttcctttgt gtttttcaga ttgttttaac
    17941 tcttctgata attccaaata tttctcatca aactcttggt cgcttgaaaa gatcttggtc
    18001 aagtcccaag ttaaattttc aggcaattct tctcgttttg gcaactgctt tacttccatg
    18061 aaatccactc cttttcttcc cttagctcta tctatcaaaa tagatcatag tctaacatcc
    18121 acatgtcaca gcatctaact tttctactca ttcatgtgtc tgcttccatc aattttagca
    18181 taactaaatc ttgttttaca tagaaaaccg attcctagcc tatttttata cttactattt
    18241 tttcaaagaa gacaagtgta tgcattcgtt aaaaaactgc tgatagacca tctcttcatc
    18301 aatcatagga aaaagccatt cccttttatg ttcttttatt tggcaaagcc atgagttgaa
    18361 atcaggaact ttctggtttt ccaaggcttc ttcgtacaac attcgataaa gaaaaacctg
    18421 ttcttgtaaa taaaagaaaa accgactttt ttgatagatc cacgatgata gatgcaaaag
    18481 atgtttatga cgcaagtagc attgttcttg tatggatact gtattctttt gcttgtgaat
    18541 aagttggcgt gtcagccatt tcttcccatc ttcactcact ttttctattt ttttcatttg
    18601 gttcatttta atttcgttac tagtgaatat ttctttaagg tccctactag aaaaagtcca
    18661 gcagctactg caaaaggaaa tatgccctga tacagtctct gtaatatgat gatacaaata
    18721 aagcttttgt ttcttgagat cagctttcca gcaatgcact cctctttttc tattatagta
    18781 gcagcaactt ttttctatca atgagaattg cgattgtcca agaaaagaat taccaagtat
    18841 ccaccatact tggtaactgt tattcaaata attttgggtt ctttcaataa atctctggtg
    18901 agacagtcta gaacattgga tttctattgc tagattcgcc aacaaaatat ccggcctttg
    18961 ccgcaacctc ggcaaatagg cttccaaaaa tactggttct actgactgtc ccagccaatc
    19021 catgaagcat tcctttaact gtaagtgctc ttttgattct ctttcactaa agaccgaaca
    19081 gttatctgat ttttgatggg caaaatgagc acattttttc tttccctttt tcaaaatcac
    19141 tctttttcga cagccacgac aataatattt ctttcctttt tctgcatgtt tagcaaaaca
    19201 gcacttatta tattcattca ttgcaagcag catttttctc cctctgaact tatttacgtc
    19261 taagaattta tttacacgaa aaaagagggc gtgaaaaagc tattctgcat caagtaataa
    19321 gaacagctaa acaaaaatag ttcccaatat ttttcgtttg gttgggcttg ttaccgcaga
    19381 tgcaagcttt tgaacacatt tatacgaaaa aaagactgcg acacaatttt tgtcacagtc
    19441 tcttactata atgctgattt atacaaaata aaatttcgtt cgctcgatag cattacgcgc
    19501 catcagacat tgtccatatt cattcaatgt ttctggtgta cgatcgctct tttcagcaaa
    19561 ctccaaaatt cgagcaatct gattctctac atccgcttgg ttgagatttt ccatccagaa
    19621 gtgaacgact aggtaatagc gctcttcgaa taaatacaaa tcagtccaag cagactctag
    19681 ttcgacatct gttgcaagtt ggatcacttg ttcaaaatct tcaaaagcaa aagttgcttg
    19741 tctttcctgt tcttctaggg cgcttccaga taattcttcc tccggatcgc tgccaataat
    19801 ctgctgacga agaaagtcac tcacatcttc tgaatcaaca gtagaatctt ctgattgaga
    19861 aatttcttca tccggaagat ttttactgat aaacagttct aatccgtctc cttttggcaa
    19921 aacttggaaa gtgacagctt cgcttcctcg gaactcctct tcgatatcaa cttcttccaa
    19981 aatactataa aagaaatttt cgatttcttt gtgattgcca agtaaatcca aaaatgaaac
    20041 gccccgttcc tctaaatctt catgaccaat tagtacacgg atcgtatttt cattaatatg
    20101 ttccatttcc attttgctac acctcacttt gccacaatgt ttaatataga ttcattgtag
    20161 cgaaatcagc ttaaatgtaa agaaaaaaaa tctcgctgtt aagcgaattt aataaaacca
    20221 gcggattttt caacctttca agctatctta tttaactata acatttttgg cagacgaata
    20281 ctatatagcc gacttttcag ctaattttcc ctctctattt ttcgtgcaat catcatcttc
    20341 tactaccttt ttatacatct aatcaagaat actttttgta aaaacaaaaa aacttggaaa
    20401 aactatcttt gt
//
LOCUS       DARDHA010000035        19861 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000035 DARDHA010000000
VERSION     DARDHA010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.035"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            195..452
                     /locus_tag="SDA10_001051"
     CDS             195..452
                     /locus_tag="SDA10_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239977.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            565..1176
                     /locus_tag="SDA10_001052"
     CDS             565..1176
                     /locus_tag="SDA10_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239978.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            1355..1912
                     /locus_tag="SDA10_001053"
     CDS             1355..1912
                     /locus_tag="SDA10_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239979.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(2611..3132)
                     /locus_tag="SDA10_001054"
     CDS             complement(2611..3132)
                     /locus_tag="SDA10_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEG4239980.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            3331..7023
                     /gene="nifJ"
                     /locus_tag="SDA10_001055"
     CDS             3331..7023
                     /gene="nifJ"
                     /locus_tag="SDA10_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEG4239981.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            7212..8099
                     /locus_tag="SDA10_001056"
     CDS             7212..8099
                     /locus_tag="SDA10_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEG4239982.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            8096..9265
                     /locus_tag="SDA10_001057"
     CDS             8096..9265
                     /locus_tag="SDA10_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239983.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            9289..10644
                     /locus_tag="SDA10_001058"
     CDS             9289..10644
                     /locus_tag="SDA10_001058"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEG4239984.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(10716..12521)
                     /gene="glmS"
                     /locus_tag="SDA10_001059"
     CDS             complement(10716..12521)
                     /gene="glmS"
                     /locus_tag="SDA10_001059"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEG4239985.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            12930..13220
                     /locus_tag="SDA10_001060"
     CDS             12930..13220
                     /locus_tag="SDA10_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239986.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            13233..14258
                     /gene="sppA"
                     /locus_tag="SDA10_001061"
     CDS             13233..14258
                     /gene="sppA"
                     /locus_tag="SDA10_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEG4239987.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            14261..14920
                     /locus_tag="SDA10_001062"
     CDS             14261..14920
                     /locus_tag="SDA10_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG4239988.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            15077..16009
                     /locus_tag="SDA10_001063"
     CDS             15077..16009
                     /locus_tag="SDA10_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239989.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            16163..17452
                     /locus_tag="SDA10_001064"
     CDS             16163..17452
                     /locus_tag="SDA10_001064"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEG4239990.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            17699..18949
                     /gene="clpX"
                     /locus_tag="SDA10_001065"
     CDS             17699..18949
                     /gene="clpX"
                     /locus_tag="SDA10_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEG4239991.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            19032..19619
                     /locus_tag="SDA10_001066"
     CDS             19032..19619
                     /locus_tag="SDA10_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEG4239992.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(19693..19848)
                     /locus_tag="SDA10_001067"
     CDS             complement(19693..19848)
                     /locus_tag="SDA10_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239993.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
BASE COUNT         6552 a         3443 c         4328 g         5538 t
ORIGIN      
        1 gtttattgat aataatgtat gagatacagt ataatgattt aaaaggtaac gtgttgtgac
       61 cgaattaaat gaattaatgc ctatttttta tattccttag tggacaaaaa tggagacagt
      121 gaagttagga gcattgtatc ctttgttgcg tagttcaaaa aagtaagcga tttctataat
      181 ggtcagggta aacggtgagg gctgtccacg taaatcctac tattgaaatc acaatgatcg
      241 gacattcaaa aggtggtgct gaagcagcaa attcagtagc tataaatacg aatgcgatta
      301 tatttaatcc agcgacagta aatttatctg cttatgaatt agactcaaac acatatacag
      361 caaacatggt agcttatgtt gtcgagggag atgcattaaa tgttgttttt ggaggagtat
      421 acacctattg gagaattaat catgttagat agacaatatg atgcacctta gtggagaatg
      481 atatctcgca taggtaactc agtaaaaaat caaggtatgg aagcaattat ctctgcattg
      541 gaagatcgat atagataggc ggatatgaat agtaataggg ggaataaaat ggaatacaat
      601 ggatcaatga gaattgtaca aattatattt tggattacat cgggaattgt gatcatagga
      661 ggagtttttt tgatgttgcc aagcttaata tttcctttct ttgcattaat ctcacctaaa
      721 atacccgaac cggaaatcac ctatggagaa tttccattta ggattgaata cgaactagat
      781 ggacagatta acataattga agatactgtc atagctgagt ttaacgggtg tgagttcagc
      841 gcaggttcta tgaaaagaga acgaagatgg agaagtagat tagctagtgg tagagaagat
      901 ctaccttttg gggacgatct aggaatctat tttagtcgag gaagtgcgca atattatatg
      961 ggggaaaatg tacaatcaat gtcattaaaa ccacatatag cccttagaaa tctcgaggaa
     1021 ggttttaaaa gagaggtaga ctttatatta ggagaaagtg gatacataag aactgtactt
     1081 aatttcggag aagcgcaaga agttttgaca caatacggaa ttaccttaat caactgggaa
     1141 atatctgaac cgattgttaa caattttggt gattagaaaa caattacgat aaatagaaac
     1201 aagccaaatc atcagcaagt aaaagctgat gatttggcct ttcttatacg agtagtagga
     1261 attcatagcg aatattatta cgaccagtag atattttgat attaaaggag tattacgaac
     1321 taatatgtga gaatgtagag gtgatgtgcg aataatgaga aatttatcaa atagaaacaa
     1381 aatattaatc attattgttg ttatagctgt gtttcatctg ggaacgaatg cggttttaag
     1441 tagaataata ttgggtccta agccaccaag accagaaatt acccgagggg agtttgattt
     1501 tagattagaa tatgaagtag atggggaaag gattgtgata gaagatacaa ttgttgcatt
     1561 atttgatgga ttttctgcag atgcaggtag catggcatgg tatcgcacgt ggcgtctaca
     1621 tttagcaagt gatagacgat cacgaaatat cttattagat gaattagagg atggtcgaag
     1681 aatatattat gtcccagaaa gtgctaacta ttctatggga gatgtacaga aaaaaagaga
     1741 gcctaatcct aattggtatc catttaatgg cgtaacaata gaatatccac gcaataaaac
     1801 acctgaaata ggtgcaaaat ttataagtgg tttagaagat ctgtacgata ggtttggaat
     1861 ccgtcttatt agttgggaac acgatccacc aatagaaaat cgctttgaat aatcgaaatc
     1921 tactaatcca tttcatcagc cagtgaatgt tgtaatggaa caatttggtt tagtgttcaa
     1981 aaccattgaa atactaggct gtgatcagca aaaatgaatc tgaataaaaa agagagcaac
     2041 tattagctga gaggagttaa tagttgttag ctttatacga atcaaagtgg caaatacaga
     2101 tgcgtatgca tttgtgtttg ccatcttgcg tttggtagac gagataggta taagctaaag
     2161 aaacaacttg tttaaaataa acgacctagc tcaacaaatg accatctaat caactaacag
     2221 catacataaa agcaactatt tttgaagtag aattgaaatc gggataactt aaaaagtgat
     2281 tgggatacta gtgagaatca ccgtacataa atattactcg ttaagggaaa ttgagaaatt
     2341 ttgtatgaaa atttaagaat tagttagtat gcgcaagtca aaaatatgaa taaattattt
     2401 ggtggagata atttgcttag cttgtcataa gaaatgagat aatctggcag atgcaagctt
     2461 ttggacacca tttattcgga ataaggaggc tgggacagaa gcgtttaact ccaagaaata
     2521 agaaggagtt gcttctgctt ccgccgttta tacgtgttta gagcgtgaga caaaagtgtt
     2581 ctttactttt gtcccacgct ccttattttt ttattttgtt tggatcaatt cttttaattc
     2641 ctctattgaa atagattcat caaagatttc tttacccaag atgacagttg gaacaaatcg
     2701 aatatgtgct tgctcagctt cgttgatgac agcttggctc agctgttcgt ctttttgttc
     2761 tgtcagcttt agtttatcta ctgcaaaatc cgctacttct tggagagaaa gatgtttcca
     2821 ctcgtcttga gtatcgaata tttgtttgat ctctttgatc gctttttggc catcgctgat
     2881 tgtgagatac cggtgcatca cgttccctcg ctgtaagctt tctttttctt tatcgaacaa
     2941 tttgatcact cgctgcaatt gcccgctttg gactgcttct tctaatagct cataagattc
     3001 ttcaaaccat tgtcggcaat acggacaagc taagttgatg aattcgacca tttttttagg
     3061 agcattcgat gaaccataaa ggattccttt ttgtgtattt gttttcgttg catcaatcac
     3121 tgatatatcc atgttaaaac acctctttcg cttatagttt atacatgtct gacaaaaaaa
     3181 ggcaacgatt tagactgttt gtctaaaaat tagatgacca attcaaatag atacatgtaa
     3241 aatattttta taaataaaag aaaaaaatgg catgttttac gttataatgg aacagataat
     3301 aagagagaat atgaacaagg atgatgaatc atgcgaaaaa tgaaaacaat ggatggcaat
     3361 acggcagccg catatatttc ttatgcattt accgagattg ctgcaatata tccaataaca
     3421 cccagttcga ccatggctga attagtagat caatggtcag cagcaggaaa agaaaacgta
     3481 ttcggtcaac cagtaaaagt agtagaaatg caatccgaag caggagcagc tggtgtggtc
     3541 catggatcat tgaaaactgg tgcactgaca actacatata ccgcttcaca agggctgctt
     3601 ttgatgatac cgaatatgta caagattgct ggggaactac tgccaagtgt tttccacgtt
     3661 gctagtcggg cagtgacgac aaatgcgttg aatattttcg gagatcacgg agacgtgatg
     3721 gctgcaagac aaacaggatt tgctatgtta gctgaaagca gtgtacaaga agtgatggat
     3781 ttagcacctg ttgcccatct tgcttctatt gaaggagaag tccctttttt gaatttcttt
     3841 gatggattcc gtacgagtca cgagattcaa aaaatcgaag ttttggaata tgaagaatta
     3901 gctcctctga tcaatcagga gaaattagct tcatttcgta aacgagggat gaatcccaat
     3961 catccgactg ttagtggtac gaaccagaat ccagacatcc attttcaaca acgggaaacg
     4021 atcaacccct attatgataa attacctgaa atcgttcaaa aatatatgaa agagatcaac
     4081 cgattacgag gaacttctta tgacttggtg acttattacg gagcagacga tgcggaagaa
     4141 gtcattgttt ctatgggatc agttgcacag acgattgaac aaacgattga ttacttgaat
     4201 gcaaacggac ggaaaacagg ttttttgaat atccatcttt atcgtccttt tccagtggaa
     4261 acattcgtgc aaaagttgcc tcatacagta aaaacaattg ctgttcttga tcgaagtaaa
     4321 gaaccagggg caggtggaga acctctctta ttagacgttc aaagtgcgtt atatgattct
     4381 gaacttcgcc cggctgttat tggtggtcgc tacggattgg ggtctaaaga tgtgactccc
     4441 gaccaaatct cagcagtttt tgatgaattg aaaaaagacc caagcatacg caaaaaacgg
     4501 tttaccatag ggatcgtgga tgatgtgact tatcaatctt tggaaccaaa ggaaagtctg
     4561 gatttgacag aaccgcaaac attccaggcc aagttttggg gatttggatc tgacggaaca
     4621 gttggagcaa ataaatcagc tatcaagatt attggagatc acaccgataa atatgcgcaa
     4681 ggctattttt attatgattc gaaaaaatct ggcggtctga ctgtctctca tcttcgtttt
     4741 ggagatacgc cgatacgttc agcgtatctt gtcgagcatg cggaccttgt tgcctgtcac
     4801 acaccagctt acctgcatag ctatgacttg gtcaaaggat tgaagccagg tggcattttt
     4861 ttattgaata ctctttggag cgacgaacaa ttagaaacgc atctgccctt aaaattaaaa
     4921 cgttacttgg ctgaaaataa cattagattc tatacgatca atgccatgag attggcccaa
     4981 gaagtcggtt tgggacgccg gatcaatacg gcaatggaaa cggctttttt taaattagct
     5041 gacatcattc cttttgatga ggtgcttccg ctgctaaaag aagaagccct gaaaagctat
     5101 ggacataaat caatggctat cgtagaaaaa aacatacaag caatcgatcg gacgaccgaa
     5161 ctgcttcatc aagtcgcgat cccagaccat tggagaacgt tggagatgcc tgaaaaaatc
     5221 aataaaagga acactggtta tatacaagaa atacttgaac cagtgaatgc acaggaagga
     5281 aatcaactct ctgttggtac acttgtagca aatggaatga caactggcga aatgccttta
     5341 ggaacggctt ctatggaaaa gcggggcgtc gctttagaag tacctgagtg gattagtgat
     5401 cgctgtacga tgtgtaatga atgtgccttt gtttgtcctc atgctgccat ccgtccattt
     5461 ttagcagatg aagaggaaat ggaagaagcg ccagaaggat ttatcgtgag agaaatgcga
     5521 ggcgcagacg gcgtaaaata ccgtatccaa gtttcagtgg aagactgtac aggttgtggt
     5581 ctgtgtgtag aggcttgtcc cgcaaaagaa aaagctttag tgatgaagcc ttatgaagaa
     5641 cagaaagaac aagctgtgaa ttgggctttt gcaatgacat taagacaaaa agcaaatcca
     5701 gtcaaaggaa agaatactgt cttgtctaca caattcaatc aaccgcttct tgaattctcg
     5761 ggcgcttgct caggctgtgg cgaaacacct tatgtaaaat tattgacaca aatgttcggt
     5821 gatcgaatga tgatcgcgaa tgcgacaggc tgttcttcta tttggggcgg tgcttctcct
     5881 gcttctcctt atacgaccaa tgaatgtggt caagggcctg catggtcaaa ttctttattg
     5941 gaagataatg ctgaatttgg ttatggtatg cttctagcgg cacagactcg tcgcgaggct
     6001 ttggcaagtt caatcaaaga agtattgcca aaagtatcag aagaactacg agaattgatg
     6061 gaagactggt tagtacacat gtttgagagt gaaggaacgc agcaaagagc agcaaaactg
     6121 caagctgcga tcgaaagcga acgatcaatt ttccctgaac tggaggatat atacaagaga
     6181 aaggatctat tcgtaaaaaa cagccaatgg atgattggtg gagacggatg ggcttacgac
     6241 attggttttg gcgggatcga tcatgtactt gccagcggag cagacgtgaa tatccttgtt
     6301 ttggataacg aagtctattc caatactggc ggtcaaacct caaaagcaac accagcttcg
     6361 gcaatcgcta aattttcggc tagtggaaag tatgcttcaa agaaagatct aggaatgatg
     6421 gcgatgacat atggaaatgt ttatgtggcg caaattgctt caggtgccaa tcaaatgcaa
     6481 acgatcaagg catttgaaga agcagaaaag tatcctggtc cttctctgat tattgcctat
     6541 acgccttgta tcacacatgg cttgcttggc ggaatgaaag aatcgcttaa agaagcaaaa
     6601 gaagctgttt cttctggtta ttggtcatta tatcgatata accctacaat ggtcgaaaaa
     6661 aacaagaacc caatgacttt ggactacaaa aaacctgatt tctctgctat gcctgatttc
     6721 atgaggaaac aagttcgctt ttcctcgcta gataacgcaa atccaaatct tgcaaaagct
     6781 ttatttgaaa agactgtcca cgacgctaaa acacgttttt acaactatgc tgcactgacg
     6841 ggagaattag aaaaaatcaa gaaacgactt gaaccagagg aatctatcga tgtacccgtt
     6901 aagactagaa aagaaaaaac gacggacctt gaagcagaag aaagaagagc tgctagaaga
     6961 gctgccagag cagaacgacg taaaaaaatg gaacaagcta caaaagaaaa aaaggaaaaa
     7021 taacagaaaa aatataatga ataattctct gaagtttaaa aatatttatt gcagagaaaa
     7081 ataaaacgca tacttgcaat tttcttgaaa attatgcgtt tttctcaaga aaaatacgag
     7141 catatcagcc tctaaaatta aaacatatga tatactagag aggaaatctc aaaagagaag
     7201 agaggctttt tatgtcattt caaatcgggg aactttttaa tttggactat tggcagcaaa
     7261 tgttttccgg tgacttgttc tctctgaatt ttttggtgaa catacttgat attctggttg
     7321 tctggtatct tgtatacaaa ctgatccaac tgctgcgagg cacaaaagcg attcagcttc
     7381 tgaaaggtgt agctattttt attgttatcc gaatcgtagc ggaaatcatt ggtctgcaca
     7441 ccttgtcttg gctgatgaac caagtcatta cgtacggggt gatagctgct atcgtgattt
     7501 ttcagccgga aatccggaga ggactggaac atcttggacg cagttcgctt ttcaaacaat
     7561 caaaaagcga acagcaggaa gatgagaaga tgatcttgtc ttttgacaaa gccatccaat
     7621 atatgtccaa gcgaaaaatc ggtgcgttga tcactatcga acgtcatact ggattagatg
     7681 agtatattga gacagggatt gctttagatg cagatatcac tggggagctg ctgatcaata
     7741 tctttattcc taatacaccg ctgcatgacg gtgcagttat tgtaaaagaa ggaaagatcg
     7801 cagtagcaag cgcctatctt cctctatcag aaagtatgtt gatccctaaa gaattcggga
     7861 ctcgacaccg ggcagctgtc ggtataagcg aagtcagtga cgcgattacc atcgttgtct
     7921 ctgaagaaac cggggacgtc agcatcacgt tagataatga actgatggct ggcttatccc
     7981 agcaagaata tttggcaatc ttgcgtagag aactaattgc tgaagagaaa acaaaaaaca
     8041 agaaaaacgt gattcaatct ttcctagacg gtatgactaa gggaggggat cgcaaatgat
     8101 ctcaaaagag aaacggacaa gcattcttta cagttttatt gcattgctgt tcagtattgc
     8161 gctgtacttc aatgcaaatg gccagtccgt tccaaatacg ctttctggaa atgaagcgta
     8221 taatcaaaca gttacggggg tgccgatcaa gatttcttat gattcaaagc gctactatat
     8281 ccatggatat gaaaatgcag tgactgtcaa actttccagt gcaaaccgag ttcagttgaa
     8341 tgcagaggca aacgaagata cacggatgtt ccgcgtcaca gcagatatca ctaagctggg
     8401 cgaaggaaca catgaagttc cgctaaaaat tcaaaatttg agcagtgctg tcacagcaaa
     8461 gatcgaacca tcgactatta cagtaacggt tgagaaaaag gtaataaaag atttcgatgt
     8521 ggaaacgttg attccttcaa cgttcacacc atctggatat gaacttgatg atacttccgt
     8581 tagtcctaaa aaagtctcta tcactacagg tgacaaaaca ttggcagaaa tcaaacgagt
     8641 agtagctgat gtggacgctt cgatggtcac agatgatggg attaatagtg aggtcccaat
     8701 acaggcgctg aatgcagcag gggaagtatt aagtatcgtc tctgatccag tgcaagtaac
     8761 agtgagagct gattcgatca agccaagcaa gtcggtacga ctttatggga tacagcaagg
     8821 aacaccagct gccggggtag aaagctatga tttcagtttt tcaaaactgg aagcagaagt
     8881 aagcggctct agcgatttgc ttgcatcgat cggtgacagt attgctgtac cgatcaatgt
     8941 ctcaggtatc aatcatcgaa caactcgtaa aattgaaata ccagtagaag aggggttaag
     9001 tatcaagccc gaatcagtta gtgtggagat tacacccgtg ttgcaaacgt caagcagtag
     9061 ctctgcttcg acgagcagtc ggacacaaga aaatacaagt acgagtagaa gtatttcatc
     9121 tgctgattca ggaacggaac catcgacaac aaccatttct tccagttcgg aatctgggac
     9181 aagcagctcg accacaagta cagaagaaac atcagaaaca agcgaatcaa cacaaatgag
     9241 cagaacaaat tcctcagaaa attaagttaa aggagtaatt ttacaactat gggtaaatat
     9301 tttggaacag acggcgtacg cggtgaagcc aacaaggaac ttacgccaga acttgcattt
     9361 aagctgggac ggtgcggagg ttatgtccta agccagcatg aagaagaagg aaaacgccca
     9421 cgcgtgttag tagggcggga tacaagaatc tcaggtcaaa tgttagaaga agcgctgatt
     9481 tcaggacttt tatcggtggg tatcgaagtg ttccagctag gtgtgatctc tacaccaggt
     9541 gttgcttatc tgacacgttt gcaaaaagca agcgcagggg tcatgatttc tgcttctcat
     9601 aatccagcac aagataatgg aatcaaattt ttcggtgcag atggttttaa actagtagat
     9661 gaccaagagc tagaaattga agcattatta gatgctgaag tggatgaatt gcctcgtcca
     9721 tcagctgaag gattgggaac tgtagaagaa tttccagaag gcttattgaa atattctcaa
     9781 ttccttgtac aaacgatcaa cggtgacctt tccggtttga ccatttgtgt cgatgcagca
     9841 aacggagcaa cttcaacaag tgttaatcgt ttgtttgccg atcttgaaac agatttttat
     9901 acaatgggca caaatccaga cgggttaaat atcaatgacg gcgtaggctc tacccatcca
     9961 gaacgtttag ctgagatggt cgttgaaaaa ggagcagatg ctggactagc tttcgatggc
    10021 gatggcgatc gtattattgc ggtggatgaa cttggaaaca ttgtcgacgg tgataaaatc
    10081 atgtttatct gtgctaaata cttggcagaa aaaaatcgat tgaaaaaaga tacgatcgtt
    10141 acgactgtca tgagtaactt aggattccac aaagcggtgg aaggaatcgg tttgaaagat
    10201 gtcgttacac aagttggtga tcgttacgtt gtagaagaaa tgcgcaaaaa cgattataac
    10261 tttggcggcg agcagtctgg gcacatgatt ttcttagatt ttaacacaac tggagatggg
    10321 atgctttcag gaattcagtt actgaatatc atgaaacaga caggtaagaa attatcagag
    10381 cttgcagcag aggtaacaat ctatccgcaa aaattagtga atatccgcgt aagtgataaa
    10441 aacggtgcga tggatgttcc ggcaatcaaa aaagtgattg aagaggcaga agctgaaatg
    10501 ggatcagaag gaagaatcct agttcgtcct tccggtacag aaccattact tcgagtgatg
    10561 gcagaagcgc ctactgatga gaaagttaat tactatgtcg ataaaatcgc ggcagtagtc
    10621 aaagaagaaa tcggactgga ttaagagata ttttcataaa aaataaaaga acaaggacca
    10681 gtgtcagatg tctgacactg gtccttgttc ttttattatt ctactgtcac tgatttcgct
    10741 aaattacgtg gtttgtcaac gtcgaatccc cgtaatagcg ttgcgtaata tgcaagtaat
    10801 tgagtcggta cgactgtggt tagagtagtt agataagggt ggacagttgg tagcaccagc
    10861 tggtcacttt ccttttctaa tccctctgag acaataacta gtgttttagc gccacggctt
    10921 tcgacttctt tcaggtttcc tcgagtatgc gcaccagtca cttcttcact gatgattccg
    10981 atcactggtg ttccttcttc gatcaaagca atcgttccat gtttcaattc gccagcggca
    11041 aaaccttctg cctggatata actgatttct ttcaatttca aagcagcttc aagagaaacg
    11101 ttgtagtcag caccacgtcc aatgtaaaag gcatttcttg tatcgctgag atatacttct
    11161 gccaactctt ctaaataatc tttttgggcg atgaccgatt ccatcgcatt agcaactaaa
    11221 ctcaattcat gagtgatatc gaatacaaga gaagtttcaa attctttttt atcaccgata
    11281 gctttggcta ataaagttag tacagctatt tgtgcagtat aagctttcgt tgatgctact
    11341 gcgatttcgg gtccagcatg caatagcagt gtaaaggatg cttcccgcga aagagtagaa
    11401 ccagcaacat ttgtaatcgt aagtgaaggg tagccgagac ggttgatttt taccagcact
    11461 tggcggctgt ctgctgtttc gccactttga ctcaagaaaa taaagaatgg tttttttgaa
    11521 agaagaggtg tgttatagcc aaattcactg gctaaatgaa cctcaacagg aatctgtgtc
    11581 aatttttcca aagtctgttt acctgctaac cccgcatggt aactagttcc gcaagcaacg
    11641 atatagattc gatcgctatc agacaatgta tcgaccagct tttgatcaag agagacacga
    11701 ttgtcttctg ttacgtattt ttgagcgata cgacgcatga taataggctg ttcgtcgatt
    11761 tctttcagca tataaaaagg ataggtacct ttctcgatat ctgaagcatc tacttgtgct
    11821 tcgaatgaag aacgttctac gatgtcacct gcaaaatttt gaataataac tttttctgat
    11881 gtgacggtaa ccatctcttc atcttcaatc tcaacgaatt ccttcgtatg agcaagcata
    11941 gccattgcat cgctggcaat cacattgaat ccatcgccga gtccgattaa tagtggactt
    12001 ttatttttag cgacgtaaat aacatcaggt gctgtccgat caatcaaagc gaaagcgtaa
    12061 gaacctttga tgacccgcaa agcttttaaa aacgcctctt gcgttgtagt atccttcgcg
    12121 aaagtttcaa ttagatgagc gataatctca gtatcggtct caccaataaa gtggtcattt
    12181 gctaaataag caattttaag ttcttcaaag ttttcgatta cgccgttgtg aacgagcacg
    12241 aagcgtccac tttgggaagt atgtggatgg gcgttttctt cagaaggttg cccgtgagtt
    12301 gcccatcgtg tatgaccaat acctatattt cctgtagtat caatatttat tttgtttttc
    12361 aaattttgaa ttctgccttg tgctttgacc agataatcag ttgtgccatc tgagacaaag
    12421 ataccagctg aatcataacc acggtattct aatttttcta gaccgtcaat caatcctggt
    12481 gttacatttt ttaatccaat cattccaaca attccgcaca taaaaaacat cctactttct
    12541 ataatgaatt cgtatgaatg cttccagctg caggaaaatc cttttgtttt atctttgcag
    12601 acattttttg tggattttct gtagagtgca gtccctaggg ccatccgccg aatctttcga
    12661 taaaccctct cctcgtcatc tgtttacaca gacctggcgc tatcctaaaa ggtataacta
    12721 tcctcctttt tgttttttat ctggaaacat ttaaaatcat aaccttttgc gaatgttctt
    12781 gtcaacaaaa aaagaataaa aaataaattg gtatatatca aattctttgt caaatgtcct
    12841 ttttatgtca aaaaataatg ggtttttatc tatcatcttt ttcaggagtt cggtacaata
    12901 gaaagtaatc ggatagagga ggagtaaaaa tggattcaaa aattgagatc atgacactgg
    12961 gaatgttaaa aaaacaattg tccaaattcg aagcatcagc tggtgtatca gatgatacga
    13021 aaatattttt agatactggc tgggatagca tccaagaaat cgcacctgat gccttggaag
    13081 ttgctcaagc tcgtgagttt acggtagaag atgaattaac aaaagagtcg ttttccgggt
    13141 atgcgcggga agaaaaagca gagcggttcg acacttctga gcagtcagaa actgtgatcg
    13201 tcataaaaaa cttgtattag aaaggagaaa gaatgaataa aagacgttgg gcagcagttc
    13261 tgattgctgc aggattattt gtggtttcgt tagtttcagc tagtttgact aagcctcagg
    13321 aaaacgatca aattgaggga ttgaacagtt ggttatatgg ggatgaagaa ctagctccaa
    13381 atgtgttgga agagggagac agtacgcaaa aaatcgtaaa attatcagta gatggaacga
    13441 ttgctgcggg agggtcttca ggtttatttg cttctgaagg ttacaatcat aaaaatttta
    13501 tggaacaact gaaggcgatt gaagaagatc caacagtcaa aggtatcttt ttagaagtaa
    13561 attctcctgg tggcggtgtc tacgaaagtg cggaaatcgc taagaaattg gatacgattc
    13621 gtaaagaaca tgatatccca atgtatgtca gcatgaaaaa catggcagct agcggtggct
    13681 actatatttc tgctcaagca gataaaatct ttgcaacaga agaaacagtg accggctcaa
    13741 ttggtgtgat catgtcagga ttgaattatt caggacttct agaaaaactg ggtgtggaag
    13801 atacaacagt taaaagcgga gcgttgaaag atatgggttc aggaacgcga ccagagacaa
    13861 aagaagaaga agcggtgctt caagcatata tcgacagcgc atatagtcgt tttgtagcaa
    13921 tcgtcagcga gggacggaat aaatctgaag aagaagtcaa aaaaattgct gatggaagga
    13981 tttatgatgg tgtccaagca aaagaagtag gtttagtcga tgaattggga tttcctgaag
    14041 atgctttaaa agctatacga aaggaacaac agcttgaaga tgcggaacta gtagaatatt
    14101 cttccacgcc atctgggttt gctagtacat ggttaggaag taaactagca gaattccagg
    14161 gattgaagcc ttctgagact agtcagatct tatccgttct tgagaatcta gggacagcgg
    14221 aatcaccaaa agcaatgtat tattacggag gtgagtaagc atgtcagagc tttcaaatga
    14281 aaataaggaa caagaaaaag ataagcaaca gcagcctgct gtaggacctg actccaactt
    14341 ttcccaaaag gtactgagct cttttgaaag agagcctttg acgcccgaag aaatcaaaaa
    14401 gaaacaacgt aaatggaaaa agtatacgga cacaagaaat gaaccgccaa aagcaaatga
    14461 ttttcctaag tatttctatg caggtttttt tatccgaatg tttgccttct tggtcgatct
    14521 tttgtgtatc ggagctatta cgcgtttaac acttggtatt gccaaaaatc taggttggct
    14581 taatttttca gattcttatc tgagtctcta cggattcaca gccttactta tttatctagc
    14641 gtattttatt ttattgacga aattcaatca tggccaaacg attgggaaaa tgatctttgg
    14701 tattcgagtc atcagctttg atgaagaaga acttagctgg tcaaccgttt tagttagaga
    14761 aggtgcttgc cggttcattc taaaatttcc tttgctgatg ttagggtatc ttccagcagt
    14821 ctttaatcag aaaaagcaac atctgggcga ttttttcact aatacaagtg ttgttacgat
    14881 caatctgatc aaagcgttca accaggaagt caacagctga tgagcaataa aaagcagtaa
    14941 cagttgaaag caccttctaa aagtcagatc taaaatctag cttttagagg gtgcttcagt
    15001 tatttttaat tgtcatgctt tttttgtttt gctactattg aacatgagct gaatatgagc
    15061 aagaggagga cactgcgtga agccgattga ttttccagaa aaatatgaga atttaatgcg
    15121 agtggcacaa caagcactag caaaccagca atatcagcag gcaaaagaat tatttcaacg
    15181 agcttacgag ttgaaagaaa gctttgaggc taatagcctg cttgtttttt gtctttacga
    15241 gttggacgag aaaaaagaag cattgaagca agcactcttg catgaaaaac agtatcttca
    15301 aaatgaggag tttgctgaat tttattttga cttgttaatc agtacacaag attttttata
    15361 tgctagaaaa ttgatagcaa gtacagattt ttatgaagct ttcgagcagc gggtcattga
    15421 aaaaatccaa tttgcagaag aattatccgg acaaatggag aggcagaaga tcaaggcact
    15481 ccacaataaa tcggaggaac ttccttcgtt ggaacctgca cgtcagcttg cgtctattga
    15541 tgagatcgaa cagcttcctt atcatgagtt tatccaaaca gcgagtaaac ttattgtatc
    15601 gccagaggtc catattttag caagggcaaa attactggag acacttaggc aattgaacga
    15661 atgccatcct gttttctatt tgacgattga agaaaagtta gtcaaagtca taccaaaaga
    15721 tctgccaaag ccgcagcagc aggcaagtta ccagcaatta tgtgtatttt cagatcgtta
    15781 cgggaatgaa gatgcgctgc ttagttccgt gttaaaagaa gaattcacgt tgcaatctgc
    15841 aattgtttat cctgtctatg atacatacat cgaagacccg aagcgctggt ttcaactgac
    15901 tgtagaggcg tgtacaggaa aaacgatgta tgaaggtaca gaagatgaaa aacaagattt
    15961 tttaaaaaaa agagaaaaaa ttttgcagca aatgatcttc ttccattaga ttttatcagt
    16021 gaaaaagata aaaatgttat gaaaaacaag gattctatcc attcaatatg tttacaattt
    16081 gaaagggtta gtgtactatt taaaggtatg catgaaacgt ttttgatgta taaaatgaat
    16141 ttctaaactc ggagggaatt aaatgactgc aacatttgaa aaaaaaggca cgaatgatgg
    16201 tgttttaaaa tttacgattg cgcaagacga aatccaaaaa ggattaacaa ctgcgtttaa
    16261 taaagtgaaa aaaagcttga acgtacctgg attccgtaaa gggaaagttt ctcgtcaagt
    16321 attcaaccgt atgtacggtg aagaagcttt atatgaagat gcattgaatg ctgtattacc
    16381 agaagcttat gaagctgctg tcaaagaagc tggcatcgat cctgtttctc aaccaaaaat
    16441 cgatgttgaa agcatggaaa aaggtcaaga ctgggtaatc actgctgaag taacagtgaa
    16501 accagaagta aaattaggac aatataaaga tctagttgtt gaaaaacaag accgtgaagt
    16561 aacagatgct gacgtggatg cacgtatcaa acgtgaacaa gaagcacaag ctgaattagt
    16621 tatcaaagaa gatgctgcag ctgaaaatgg cgatacagta gtaatcgact tcgaaggctt
    16681 tgtagatggc gaagcgtttg atggcggaaa aggcgaaaat tactcacttg aattaggttc
    16741 aggctcattc attccaggtt ttgaagatca attagttggt cataaatcag gtgaaagtct
    16801 agatgtaaat gtaactttcc cagaagatta tcaagcagaa gatcttgctg gaaaagaagc
    16861 agtcttcaaa gtgacaatcc atgaagtaaa agcaaaagaa gtgcctgaat tagatgacga
    16921 attcgcaaaa gatgttgatg acgaagtatc aacattagac gaattgaaag aaaaatatcg
    16981 caaagaatta gtagaaacaa aagaaaaagc agcggacgat gcaaaagatg aagcagcgat
    17041 ccgtatggct gttgaaaacg cagaaatcgt tgaattgcca cacgtgatgg ttcatgatga
    17101 agtacatcgt tcaatggacg aattcttgaa caacatgcaa cgtcaaggta tctctcctga
    17161 aatgtactat caattaactg gttctacaga agaagacttg cataaacaat tcgaaggtga
    17221 agcagaaatg cgcacaagaa caaatcttgt gatcgaagca attgcagctg ctgaaaatct
    17281 tcaagcaaca gaagaagaaa tcaatgcaga aatccaagaa ttatcagaaa catacaacat
    17341 gccaatcgaa caaatcaaac gcgtgttgac agaagatatg ctggaacatg atgtgacaat
    17401 gaaaaaagca atcgaattga tcacaggtac agcgacagaa gcgaaagaat agaaatcaag
    17461 taataaatca tttctgaaga agctacttct agaaaattgt ttattcgaaa taagggactg
    17521 tgacacgatt ttgtcacagt tccttatttt ttgttaagag taaaaaatgc aaaggtatag
    17581 gtcagttgat cctttgttaa acactttttc tttttactaa actatgttat gatagtaaaa
    17641 gactgtaaag tctttgatag tttcttgcat actttttgca agttaagggg tgaagaccat
    17701 gtatgacaat ccaagtagca atgagaccgt acgctgttct ttttgcggga aaacccaaga
    17761 agaagtgaaa aagatcgtag ctggtccagg ggtctacatc tgtaatgaat gtatcgacct
    17821 atgtaaagaa atcattgatg aagaatttta tgaagaagct gtacgcgaat tcactgaagt
    17881 ccctaaacca ctagaaattc ttgaagtatt taataactac gtgattggac aagatcgcgc
    17941 aaaacgctcg ttagctgttg ccgtatacaa tcactataaa cgtgtcaatc agcaagcaca
    18001 agaaaacaca gatgatgttg aactgcaaaa aagcaatatc tgcttgatcg gacctacagg
    18061 gtctggtaaa acatttttag cacaaacatt agcaaaaaca ttgaatgtac cttttgcaat
    18121 cgcagatgcg acaagtttga cagaagctgg ttatgtgggg gaagatgtag aaaacatttt
    18181 gcttaaattg cttcaatctg ctgattacaa tgtggaactt gcagaaaaag ggatcatcta
    18241 tattgatgag atcgataaaa ttgcgcgtaa aagcgaaaat gtatctatta ctcgtgacgt
    18301 ttccggcgaa ggtgtccaac aagccttgct gaaaattcta gaaggaactg ttgcaagtgt
    18361 gccacctcag ggcggacgta aacatccaca tcaagaattt attcagattg atacgacgaa
    18421 catattgttc atcgtaggtg gtgcatttga tggcatcgaa acgattgtga agaatcgtat
    18481 gggcgaaaag acgattggat ttggtacaca aaaccaaaaa ctttctgaag acgaaagtgt
    18541 catgcagcat atcattcctg aagatttact aaaattcgga ttgatccctg agtttatcgg
    18601 acgattacct gttatggcag ctttagagaa attgacaaca gatgatctgg tacggatctt
    18661 gactgagccg aaaaatgcgc tagtcaaaca atatcaaaaa cttctttctc tagataatac
    18721 cgaacttgaa ttcgaacctg aagcgttgag agccattgcg aaaaaagcaa tcgaacgcaa
    18781 taccggtgct cgaggacttc gttccatcat tgaagaaatc atgatggatg tgatgtttga
    18841 tatcccttca aatgaaaaca tcgaaaaagt gattatcaca aaagaagcag ctgaattgtc
    18901 tggtgagcca acagtaatct ataaagattc agagaaaaaa gcgggttgat gaaaagaggc
    18961 tgccgtaaaa gtcattttgg cttttgcgac agccgcttgc tttattcatc accttgttaa
    19021 ggagagagac catgaaagtc catcaagctg aaattatcat aagtgcagtg gcaccaaaac
    19081 aatatccaga tacacaattg ccagaaatag ctctggcagg acgatcaaat gtagggaaat
    19141 cttcttttat caacacattg attgatcgaa agaatttagc ccgtacttcc ggtaagccag
    19201 gaaagaccca gacattgaat ttttatttga tcgagaacgc attacatttt gtcgatgttc
    19261 cgggatatgg gtatgcaaaa gtatccaaaa ctgagcgagc aaaatgggga aagatgatcg
    19321 agacatattt aacgactaga gaccagctac gtgcggtcgt ttctttagta gatttgcgtc
    19381 ataagccttc tcaagaagat atccagatgt atcaattttt gaagtattat gatatccctg
    19441 taattgttgt tgccactaag gcagataaga tccctcgtgg taaatggaac aagcatgaga
    19501 gcgaaatcaa aaaagcgttg gattttgatc gcaatgacac gtttattctc ttttcttccg
    19561 ttacaaaaca gggaaaagaa gaagcgtggg cagcaatcga acaagcaatt gaatcataaa
    19621 aagaataaaa aagacttacc agtcaaactc tttcataaaa gtttggctgg taagtctttt
    19681 tttgagctta acttactttt gtttcttgtc ttttttcttt tcattttgtg gctgtggttt
    19741 tagaaatcga tcgatctctt cttttggtgc aactgttttt tccggatcga cagcaaatga
    19801 ttcgaaaagt ttatctaaat tatctgtacg ttcaaatttc aaccccattg taaacacctc
    19861 t
//
LOCUS       DARDHA010000036        19821 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000036 DARDHA010000000
VERSION     DARDHA010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.036"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            6..305
                     /locus_tag="SDA10_001068"
     CDS             6..305
                     /locus_tag="SDA10_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4239994.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="SDA10_001069"
     CDS             378..1739
                     /locus_tag="SDA10_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEG4239995.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="SDA10_001070"
     CDS             1761..2021
                     /locus_tag="SDA10_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4239996.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="SDA10_001071"
     CDS             2035..2787
                     /locus_tag="SDA10_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEG4239997.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="SDA10_001072"
     CDS             complement(2881..3486)
                     /locus_tag="SDA10_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG4239998.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="SDA10_001073"
     CDS             complement(3539..4513)
                     /locus_tag="SDA10_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEG4239999.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="SDA10_001074"
     CDS             complement(4891..5610)
                     /locus_tag="SDA10_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4240000.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="SDA10_001075"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="SDA10_001075"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEG4240001.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="SDA10_001076"
     CDS             7161..8558
                     /locus_tag="SDA10_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG4240002.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="SDA10_001077"
     CDS             8639..9079
                     /locus_tag="SDA10_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG4240003.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="SDA10_001078"
     CDS             9227..9553
                     /locus_tag="SDA10_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG4240004.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="SDA10_001079"
     CDS             9554..10303
                     /locus_tag="SDA10_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240005.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="SDA10_001080"
     CDS             complement(10383..10838)
                     /locus_tag="SDA10_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEG4240006.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="SDA10_001081"
     CDS             complement(11265..11540)
                     /locus_tag="SDA10_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240007.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="SDA10_001082"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="SDA10_001082"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEG4240008.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="SDA10_001083"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="SDA10_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEG4240009.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="SDA10_001084"
     CDS             14770..17436
                     /locus_tag="SDA10_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG4240010.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="SDA10_001085"
     CDS             17800..18552
                     /locus_tag="SDA10_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG4240011.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="SDA10_001086"
     CDS             complement(18574..19728)
                     /locus_tag="SDA10_001086"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEG4240012.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT         6329 a         3472 c         4060 g         5960 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa atggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat t
//
LOCUS       DARDHA010000037        19575 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000037 DARDHA010000000
VERSION     DARDHA010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.037"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..596
                     /locus_tag="SDA10_001087"
     CDS             <1..596
                     /locus_tag="SDA10_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEG4240013.1"
                     /translation="CQSNTNQGTSGNTSSSSEQRSSQVSDSSTKASTSSSSQTKSSSE
                     ASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYFYKIEGVDDQKEYEARIDA
                     QNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAAKIAVDKVGSGTATDWDLD
                     KDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            680..1624
                     /locus_tag="SDA10_001088"
     CDS             680..1624
                     /locus_tag="SDA10_001088"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEG4240014.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            1759..3210
                     /locus_tag="SDA10_001089"
     CDS             1759..3210
                     /locus_tag="SDA10_001089"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEG4240015.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            3231..4067
                     /locus_tag="SDA10_001090"
     CDS             3231..4067
                     /locus_tag="SDA10_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEG4240016.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            4067..4726
                     /locus_tag="SDA10_001091"
     CDS             4067..4726
                     /locus_tag="SDA10_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG4240017.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            4852..5541
                     /locus_tag="SDA10_001092"
     CDS             4852..5541
                     /locus_tag="SDA10_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240018.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            5547..6503
                     /locus_tag="SDA10_001093"
     CDS             5547..6503
                     /locus_tag="SDA10_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HEG4240019.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            6547..7059
                     /locus_tag="SDA10_001094"
     CDS             6547..7059
                     /locus_tag="SDA10_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4240020.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(7315..7863)
                     /locus_tag="SDA10_001095"
     CDS             complement(7315..7863)
                     /locus_tag="SDA10_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4240021.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(7952..9133)
                     /locus_tag="SDA10_001096"
     CDS             complement(7952..9133)
                     /locus_tag="SDA10_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEG4240022.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(9369..10877)
                     /locus_tag="SDA10_001097"
     CDS             complement(9369..10877)
                     /locus_tag="SDA10_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEG4240023.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            11036..11227
                     /locus_tag="SDA10_001098"
     CDS             11036..11227
                     /locus_tag="SDA10_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240024.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            11430..12347
                     /locus_tag="SDA10_001099"
     CDS             11430..12347
                     /locus_tag="SDA10_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HEG4240025.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            12340..13413
                     /locus_tag="SDA10_001100"
     CDS             12340..13413
                     /locus_tag="SDA10_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240026.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            13561..14079
                     /locus_tag="SDA10_001101"
     CDS             13561..14079
                     /locus_tag="SDA10_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240027.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            14060..14452
                     /locus_tag="SDA10_001102"
     CDS             14060..14452
                     /locus_tag="SDA10_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEG4240028.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            14528..15463
                     /locus_tag="SDA10_001103"
     CDS             14528..15463
                     /locus_tag="SDA10_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEG4240029.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            15596..16375
                     /locus_tag="SDA10_001104"
     CDS             15596..16375
                     /locus_tag="SDA10_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEG4240030.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            16368..16685
                     /locus_tag="SDA10_001105"
     CDS             16368..16685
                     /locus_tag="SDA10_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEG4240031.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            16929..18539
                     /locus_tag="SDA10_001106"
     CDS             16929..18539
                     /locus_tag="SDA10_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG4240032.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            18696..19544
                     /locus_tag="SDA10_001107"
     CDS             18696..19544
                     /locus_tag="SDA10_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG4240033.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         6324 a         3507 c         3943 g         5801 t
ORIGIN      
        1 cttgtcaatc aaataccaat caaggcacat ctgggaatac gtcttccagt agcgaacagc
       61 gctcttcaca ggtatcggac tcctcaacaa aagcaagcac atcttcgtct agccagacaa
      121 aaagttctag tgaagcaagt actcagtcag atatcaaagt gtctgcagct cgtgcgatcg
      181 aattgttcca agataaatac ccagatactg caattactag tttggaatta gatagtgatt
      241 ggggatctta cttctacaag atagaaggtg tcgatgatca aaaagaatac gaagcaagaa
      301 ttgatgctca aaatgaaaaa atggagacag agaatccaga aactctagat cgagatgaac
      361 aaaatggtca aaagaaaaaa gaagatggtc tggatgttga taaattgatc acgattgagg
      421 aagcagcaaa aatcgccgtc gacaaagtag gaagcggtac ggcaacagat tgggatttgg
      481 ataaagattt aggtactact tattgggaag tcaaagtaaa agacggcaat caaagtacat
      541 cagtcaagat caatagccaa acaggcgaag tactgtcaac tgaacaagat gattaagaat
      601 ttcttcggaa aacaagagtt gatttttctt ttagtcatga tacactagac aaaataactt
      661 gtttgaagga gcgatttact tgagtttaga aacgaccttt gccaatcata tctttgccaa
      721 tcctttgatg aatgcatctg gtgtccactg catgactacc caggaattag atgaactggc
      781 tcattcagaa gcaggagcat ttatcactaa gagctgtacg atcaatgaac gaaaaggaaa
      841 tcccgaacca cgctattttg atgttccttt agggagtatc aattctatgg gacttccaaa
      901 cttaggtttt tcttattatc tagagtatgc tcttgcgtat gaaaaagtgc aggaaaacca
      961 aaatcaaccg ttattttttt ccattgccgg gatgagtgtc caggaaaact tggaaatgct
     1021 tgagaaaata gaaaaaagtg ggtttaatgg gatcactgaa ttaaatttat cttgtcccaa
     1081 tgtgcctgga aaaccacaac tagcttacga ttttgaggcc acttatgaaa cactgaaaga
     1141 agtattcagc atcttttcta agccgctagg aatcaaattg cctccttatt ttgattttgc
     1201 tcattttgat cagatggctg atatcttgaa tcagtttcca ttgacctatg tcaatgctat
     1261 caacagtgtg ggcaatggtt tgtatatcga tacagaacaa gaagcagtag tcatcaaacc
     1321 aaaagaaggg ttcggcggca ttggcggaga gtatatcaag ccgactgctt tggcgaatgt
     1381 tcgcgctttt tatacacgtt tgaaaccaga aatccaaatc attggtacag gtgggattcg
     1441 gacaggacaa gatgcatttg aacatctgct atgtggcgca agcatgttac agattggtac
     1501 cgagcttcat aaagaagggc cggaaatttt ttctagaatc ataaaagaat taacgcagat
     1561 tatgtcagaa aaaggatata cgtcaattga cgaatttaaa ggcaaattac gcaccatttc
     1621 atagcaaaga gacgaaggtc atgattgatc ttcgtctttt tttataaaaa acactcgtct
     1681 gttcattcct gtgcaagcgt actcattgta tgttagacga acttgtggga aaataacaga
     1741 ggcaaaaagg aggataatat gaatgatgca acagaattag caaaagagct taaagaaaaa
     1801 gaatttagtt ttcaagaact agttacggat atacagaaaa aaataaagaa gcaaaatcat
     1861 gaattgaatg cgtttgtcgc gcttgaacca ttcgagagta attcccacga gctattgatt
     1921 ccccagtcag aaaaagggcc attggctggt atcccatttc ctttaaaaat gctcggtcaa
     1981 gaaaaaaagg gatggctagc tacttcagga tctcgtttat ttgaaacaca ccgagcttct
     2041 catacatcta attatgtgcg gcaagcagaa gcgataggtc tagttccttt tggacaaacc
     2101 aatgcgccag agtttggttt caaaaatatt acagacccag tcatctatgg cccagcaaga
     2161 aacccttgga accttgaatg tacaccgggc ggatcaagtg gaggagctgc tgctgcagtt
     2221 tcatcaggaa tcgttccttt agctggtgct agtgatggcg gtggttctat tagaataccc
     2281 gcttcgtttt gcggattgat tgggctgaaa ccttcgcgag ggacgatgcc agttggtcca
     2341 tatgcttgga gaggatggca aggagctgct attgatttcg gcttgacagt ttctatgaga
     2401 gacacggagg ctttatttta tgggatgaga agtatccatt ccggtgcgcc atatcaagta
     2461 tcgccagttg agtggcaaac acatccaaga aaaaaacggt tgaagatagc tttttgtgtt
     2521 gattcaccga tccactcaca gattagtgaa gaggcagtac ttgctgtaaa aaaagctgta
     2581 gatttccttg gaaaagaagg gcatgatttg acagagattc cttacccact agatggtcgt
     2641 aaactgatca cgtcttatta tcagatgaat ggcgcagaaa cagcagcaat gatccgctcg
     2701 attgaaacag gcatccagcg agctgtcaga aaagaagaac ttgaacccat ttcttggact
     2761 ttgttccaat atggtcagaa gctctctgcc gctacttaca tcgattctct gcatgtatgg
     2821 gatgaagcag caattgtcat ggaaaaccta catcaagaat acgatctatt cctttcacca
     2881 acgactgcat ttaccgcccc taaaatcagc gaagatttgc aaagtgatgc gataagagca
     2941 cagatggaac acatcagtga ttgttctgaa gtagaaggtt tagacgttgt ttcttccatg
     3001 tttgaggaga gcttgaagat cacaccttat acacagttgg ctaatctgac tggacaacca
     3061 gcaatcagct tgcctactca tttgacaaaa gaaaacatgc ctctaggaat ccaatttatg
     3121 gctgccagag gaagagaaga tttgctattt caaatcggct atctatttga agcaaatcat
     3181 cagttccatt taccaaatcc atggaaatag cagaaacaag gagaagaaga atgaagaaaa
     3241 aaagattgtt tgctctaata actagtgcct tttttatttt aagcgcttgc ggtacagctg
     3301 ccgctaatca ggaagataac agctggagta atgtagaaaa aaagaaagaa ttagtagtag
     3361 cgacttctgg cacactgttt cccgcatctt attataatga tcaaaatgaa ttagttggtt
     3421 atgatgtaga tttggcaaaa gaggtggcta aacggttaaa tgtcaaaatc tcctttaaag
     3481 aatacaacgt ggatggacaa atcacttctg tttcaaaaag agaatcagat tttgctgcga
     3541 acgattttgg tatcacaaaa gaaagagaaa aaaaattctc cctttcagag actttcaaat
     3601 attcatttga cagtatgatc gtacgaaaaa aagatgattc aggaatccac tcacttgaag
     3661 atcttaaagg gaaaaaagca gctggcgaac ccaatactag ctatatgcgt ttagcggaaa
     3721 aacacggagc taaattagtc acatatgaca atgccacgaa tgatcagtat ttaagcgatg
     3781 tagcaaatgg tcgaacagat gttattctaa atgattatta tctgcagaaa atggccgtat
     3841 ctgctttacc agatatccct gtaaaaatac tggagaacgt ttatttcaat gaaaacgaaa
     3901 gtggtttttt attgaaagaa ggaaatgatg ctctgaaaaa gaaaatcgat ggcgtattaa
     3961 aagagatgaa agaagatggg acgacaaaga aaatatctga aaaatatttt caaactgatg
     4021 tttcagtcaa accgaaagaa acgatggctg agtcggtgag tgagtgatgt actttcccct
     4081 aattgatttt tccctcatgt ggacttcact tcctttcgta ctgcaaggct tggtatacac
     4141 attagggatt ggcttcgtgt cgtttctttt aggaaatttg gtagggttgc ttttgacagt
     4201 tttaggtctt ttggattggc tgcctctgaa tgtatttatt cgtttttatt tatcgttctt
     4261 tagaggcatt cccgcactag tcctgctgtt tctgttatat ttcgggttac cttatcaatt
     4321 aagtgcctta acggcttcag tcatctgctt tacgattacc agcagtgcat ttattggtga
     4381 aatctatcga ggatcactag caggcgtttc ttcaggtcag tgggacgctg gtttagcttc
     4441 tggtttttct ttctttgggg tattgaaata tatcgttttg ccgcaagctt ttcgaatctc
     4501 agtaccggca ctcagtaacg tagctatgga tctagtgaaa ggtacttcgt tagcagcaac
     4561 aatcacagtg ccagaaatat ttcaaaaagc aaagatcata ggcggtcgga cattcgatta
     4621 tttttctctc tatattttag ttgcactgat ttattgggtc atttgtatcg ggatcgaaaa
     4681 agtgcaacag tatttagaaa aacgttctat ccatcagtca atctgaaaaa tagaaatggt
     4741 gctagcgtgg ctggcaccat ttctattttt tgtttcttgt atattctgcc tagtatttta
     4801 aaaccatttc atgtaaaatg taaatcgaaa atatgttcgg aggtagaaaa catgaagacc
     4861 ccgctaacca tcgaaataga atcctcgtta taccaatatt gcatcgagca aggcggcttg
     4921 gttgtagaag aagtcacgat gcctgatgac caaggaattg tagatacgct tagctgcttg
     4981 acaaaatttg atggaagccg agaatggcga tgctatgagc tgaaagtgac aaaagcagat
     5041 tttcgctcta cagccaaact gtcatttgtt ggacattata attattttgt tttaccaagt
     5101 aaactatatg aagaagtaaa aactgaaatc cctacaggaa taggtgtttt agtgtatcgt
     5161 ccgtacacgg ttgaagagga gatgcctgcc tctggtacat ttctgattgc gaaaaaagca
     5221 gcaaagaaag agcttgctgt accggaagaa gctttgacaa accgcttcat ggcttcttta
     5281 ttcagggaag taagaaaagc aaagcaaatg gagcgaggcg taaaatattt tccaaaagat
     5341 caattgtaca aagaattgaa aagacgttca gaaaatcaag atccattttc taacgaacga
     5401 tattatgaac ggtttttaga ggaaatagct agtcaggcaa tcaatgagtt gcaagaagaa
     5461 gttatcgcgc ttcagcatga ttatgaatat ttgaggcaac aacagcagat ccgtcggttg
     5521 cctacagagc cattggagtg aaaaatatgt actatcctta tttaagagga aaacaatttg
     5581 acctattagc attaaaagaa gcactgtcgc gaggattgct ctccaataaa atccaaccag
     5641 tgatcgaacc agtacgtgat tccgctacgc ttaaaaatgt gatcgaatta tttcaaaaaa
     5701 agcaccatcc aatcgctgtg atccaaaatc cccaagtagg acaattcaaa ttattcgatc
     5761 agcatgtgca cagttggaca gtcaaagaaa atagctctgt cgtccctgca cagatcttaa
     5821 cgccagaaaa tctcagtgaa gtactggatt cacctccagc gtttcttgtt ttcgatggac
     5881 agcactaccc aaaagataca gaggtgtgga aacagttggc aggagttgat tcgaaatttt
     5941 tgataccaga tactagtcgc tttcgcatct ggctgcccga aaacaaaatt gtgatccgtg
     6001 actcttttca aacgagaaag catgttgaaa gttatgcaga taaaacagat gactttttta
     6061 gtgatgacta tctttttttc cacgcagacg gatatattgg attttctgat ttcacgatcg
     6121 aaggtagccg ttatttcgat aaagggttcc ctagccgagc gcttgctatc catctaacgt
     6181 atattgatgc ttacggcaat atacgcgtga aacattttgt ctctgacagt aacgactctg
     6241 ctaaagacca agcattgaag tttcttgaag caggagataa aatgaaaaaa tggatcatgc
     6301 gtcaccatca tcaactcttg attacttcag gaatgattga attattggct ctttaccagc
     6361 aacaaaaatt ccctggactg ggagtgttga aaaagtggtc acttatgcat catttggaac
     6421 ttattagtca cctgctagat catccaaaag attggcttaa atattattca aaagttcagg
     6481 aactatatga agtgatccaa tgattagata atgtaaatga aaaaggaata gaaaagagga
     6541 aactggatgt atattcggca aacaaggaaa gaagaagtag aagaagtggt agaaatcatt
     6601 aatgccgcag cggatttcat tgcttcgcaa ggctctcctc aatggcaaaa cgggcagaaa
     6661 ccaacaaaag aaacgatttt gaacgatatt gaaagaaaag aaagctatgt attaatagtg
     6721 gaagatcaaa ttgctggaac agctgcatta gtctcaggcg tggatcccgt ttacacaaaa
     6781 atagatggta gttggaaaga atcaactggt ccttatttat cgattcatcg tgtcgcttta
     6841 gcagaaagta ctcgaggaaa aaaactagca aaaccgttct tagaacattt gttgacgatt
     6901 gcagccagca aaaatatcca agatatccgt atcgatactt atccagctaa tgaaccgatg
     6961 gaaaagacca tctattctgc tggattcact tatcaaggga tgattgaatt tccttttgaa
     7021 cacggcgaaa gaaaagcata tcaaataatc atcaattgac tataaaatca tctgagcaaa
     7081 gataaagaca ttaacactac tgaaaaaaac aatcatatga tagccaagag gttgtaacag
     7141 aagtggccag cttcaaaaaa caagaaagaa tttccgaaaa tgcttctcaa atttttgtgg
     7201 aattttagcg tattttacga aatagctgct ttctgaaact cccttcatcg agaggtcgtg
     7261 acatagcttt tgtcacgatc tctcggctta tcctacgagt attattcgat acatttatat
     7321 caataatcct ttttttattt tatctgcagc ttcttggaac gctccatcgt ttgacacgac
     7381 taaaagtgag gggatagctt tcaaacgttt caactgtgct aaatcttcta cataaaccgc
     7441 cagtctggct gaaatctgtt cagatgaatc tccacgtttt ttcaggcgcg attgcaatgt
     7501 ttctttagag atcgtaataa agataggacg gactaccgtc ccgaaatact catataaatg
     7561 ccaaaaacca tttgctgtca aaacggcgta acaatttcct actgtcagtt tctttttaat
     7621 ttctgcttta ctggaaccat atttatgttg agcatattgg tcccactctg caaactcatt
     7681 tcccttgatc atctgattga atgtattgtc agaaacaaaa taataatcct cttgatcttt
     7741 ttccccaggt cttggtgatc gattcgtgta agtaatgatc ttttgttccg gctgaaaaat
     7801 agcttctgcg agtgaagttt ttccagaacc agaaggaccg ataaatacat agcaataatt
     7861 cattgtcatt tctcctttga tgattgaaca agtgaaaaag ctttggaaaa tatttccaaa
     7921 gcttttaaaa gtaactttgc ttttttgtat tttataccag caagccagtc acaatagcac
     7981 ttaaaaagct gactaaagtc gctgttagca aaattttcaa tccgtgtttt gcgacaagat
     8041 ttcctttttc ttcatttaat cccttcatcg caccagaaat gatcccgata gaagagaagt
     8101 tggcaaatga aacgaggaag acagatataa ttgctgttgt acgctcagta aaatggaaat
     8161 taccatttgc taattctgtc atagcaacaa attcatttgt aaccagttta gtcgccatga
     8221 tactaccggc atccactgct tctttccatg ggattccgct gatgaaggca agaggggcaa
     8281 agatgtaacc taggatctct tggaaagtga tgccgaatat cccgtggaag atagcattga
     8341 tcatggcaat caaagcaaca aaaccgataa gcatagcagc aaccgtgatc gccacatgga
     8401 aaccatcaag aatgtattct cccaacattt ggaaaaatgt ctgttctttg ttttcttcaa
     8461 tgatcagctc atcttctttt tcttctaata catagggatt tacaattgaa gccagaatat
     8521 aaccgccaaa taagttcaaa acaagggcag tgatcacata tttagaatcg atcagtgcca
     8581 tataagatcc gacgatcgac atagaaactg tagacatagc cgatacactc atggtgaaca
     8641 aacgtttctc agataggaag ggtaattcct tttttatgga aataaaaact tccgattgtc
     8701 ctaaaatagc agaagctact ccattataag attccaattt gcccatcccg ttgattttac
     8761 ttaatcctag accaacagct ttcataaata atggaagaat cttgatatag cgtagaatac
     8821 cgataatagc agaaataaat acaattggca tcaatacgga aagaaagaaa ggtgcacttc
     8881 ctttatttgc tacaccggca aaaacaaagt caacaccttg cccagcaaaa gcaagaagat
     8941 gatcaaacat ttttgccaat acagaaacaa ccgctgtacc aaaagttgta cgtaataata
     9001 cgaaaccaag aacaagctgt aaaacgaaca tcacaattag tggtttcaat ttgattgctt
     9061 ttttgttact gctgatcaaa aaagcaagcc ctaatgtgac aaataaacca ataataccta
     9121 ataaataact caaataatca ctcctattcc aaattccgtt ttattttacc tgttttgcaa
     9181 ataaaatact aatctttagt gaaattttca ttactttttt agagtgaaac gcattcattt
     9241 tccattagaa aataaagcga acaaaataaa aggtttaaaa agtgtaacgt cttagtaaag
     9301 tctataaaaa aacgaaacta tcgattaagc taatagtttc gttttcaaca tttataagta
     9361 aactttttct attggtaata tagattttcg gaagggtaga caggtgcgca ggtaatatcg
     9421 attcctagtt ttttcaatgt ttgttcatca tttttattga ccatcacagt agagtgggct
     9481 tgtgctccct caagttcagc tagtttatca taagcaactt gagcgattgg attcgttact
     9541 gcactgattg ataaagcgat caagacttca ttgatgctta gtgcatccaa agaggaatga
     9601 agtgtctttg atttcaatcc ttgtattgtt tcaagaacta atggtgacaa taaaggaatg
     9661 tcatctgcga tatgggctaa atgttttact gcatttaaga taactgccgc tgacgcatcc
     9721 atcaatgaac ttgtacgccc ggtaatgatg acttcatctg gtaattcgat tgcaatcaca
     9781 gcagaaggat cttttgtagg actttgcgct tgaatcttat ctgcatagtc atgggcaggg
     9841 actaccacgt ttcggtcttc aggacgtaaa ccgacttctt ccataatcag tttgattcga
     9901 ttaactgctt cttcgtcgat caatcctttt ttgaagtcgc attctgtttg gaaataacga
     9961 cggatgattt cttgtttaga ggcttcttgg acaacggtat catccacgat cccaaatcct
    10021 acacgattca cacccatgtc agtaggagat tgataaacag attctttacc agtaattttt
    10081 tcaataatac gtttgattac aggaaatgtc tcaacatccc gattatagtt cacagctact
    10141 ttattgtagg catcaaagtg gaaggaatcg atcatattga catcttttag atcgactgtg
    10201 gctgcttcat aagcaatgtt cagcggatgt ttcaacggaa cattccatac tgggaaagtt
    10261 tcgaacttgg agtaaccagc tgcatgtcct ttttggcttt catgatacag ttggttcaag
    10321 caagtagcga gctttccact tcctgggcca ggagctgtca ctacaacgat tggtttagtt
    10381 gtttcaatat actcattttt tccaaaacca tcttctgaga cgattttttc tacgttgact
    10441 ggatagtctt cgatttctga gtgtttatag accttgatgt ttcgtctttc tagtttattg
    10501 ataaagactt tcgtggcagg ctgtccacta tagcgagtga taacgacact gttgattaat
    10561 aagccataac cacgtaattc atcgattaac cgcaagatat ccatatcata agtaatccca
    10621 taatcaccac ggatcttatt acgttcaata tctccagcat agacgcaaat cagtatttca
    10681 gcttgatctt tcaatttttg cagcaatttg attttcgcat cttcttcaaa tcctggaagt
    10741 acacgtttcg catgcttgtc gtcaactaac ttcccaccaa attctaaata taatttgtca
    10801 tagtggttca ctcgttccaa aatatacgct gactgctctt caatatattt ttctgaatca
    10861 aaaccaattt ttttcactag gttccctcct taagtactaa agcatttttt catttttttg
    10921 aggattcgtc aactggaaga gcgaaattcc tcaaaaaaac aaagaaaatg gtttaaacca
    10981 acttgtctag atagttattc attggtatga tagtttttga taaggaaggt gctacatgaa
    11041 aaaaagacag aaaaagaaaa acgcatataa acactatatc cgttcgattt ttaccggtta
    11101 tgaaagaatg cttgaagatc ccgaattgga acaattgacg tttacttatc taaacgaaga
    11161 aacgcagcta actcgtgatg agcatcagcg cattcatttc acaacaagag atctgcctag
    11221 taaataaaaa ataagataaa ggtcatcgga ttcgattctg tcctatcaga tggaaagact
    11281 agttgatcga aaaaaaggct gtaccaaatt ttggtgcagc ctgttttatg gagcattgcc
    11341 cgctcttttt tataacaatt ttttacagaa aatggtacta ttgaagtatt aataagtgaa
    11401 aaacagatga gtaagaggag gcaagtagta tgaaagagcc agatacaaat gcaaattatg
    11461 agccagacga actagaggag acgaacgcgg atcaggatac aggacaagaa tcgtcttctt
    11521 tttacgatat caaagaaaaa gcaagcatga atctttccca attattagaa gacttacagg
    11581 cttttgaaaa agctgtccgc gaagaagata tccctgagat ctaccgttta tacaatggcc
    11641 gattgaatga agaattaaaa agaagctcaa atgaaaatca cgagatcgat caattgctga
    11701 tgcgtaagat ccatgatcgt tttttacagg aatttccttt tatggaacaa gtagaaaaca
    11761 tctcgccaac gatgagctat tacaaaatcg gcacttatta ccatgatcgg cccacggtcg
    11821 gtattgatgc aagtctgcca gaaatttttg ttttgcctaa gatcgatgaa gagtgggaga
    11881 aatattccca accggagtca acggactatg acaaaaaaat caatgagcta gacgcaaagg
    11941 tgatcactgc tcagacagaa attgaacgtt tagatgaaca aataaaagaa atcaatcgac
    12001 aaatgactga tttgaacgac aataaaggat tcttgaatcg taaaaaaatc gaggaagaaa
    12061 ttcaagaatt agagaagaag aagcaagtac tatctaatga aaaattaggc tggcttcctt
    12121 atattgagac gcctgaaaca atccagcagc aaaaagaagc attgaagcaa gaagcaagag
    12181 cagaccagct tcgtgcagcc atcgtagaaa aagaacaaag gcaaatcaga cgctatttcg
    12241 gtagcaaaga gggattcggt caagcaatcc atgaattctt gatgaattat cttggcaatg
    12301 agaatcctaa taatcaatct aatgaaggag gttccgagta tgaatgaaca cgatgaagca
    12361 gaatggacaa aaaatgaaga acaaacagaa gatcatgaag aaacgaacac ttcattggaa
    12421 gaacaaacaa gtaatcctct ggatgacaca gaagaaatcc ctatgctaga ggaaacaaaa
    12481 atacatgaaa aagaaaaagt aactccgacg aatacgaata ctgcagaaaa cacccatgct
    12541 tacgaaataa ctgaaaatcc cgggtattta gaaggaaaag agcaagatca tcaagatatc
    12601 gtcagtttga ttgaaacagc ggaagcagaa ttagcggatt tacgtatgag aaaagatttg
    12661 atcgaatcag attttccaca attattagaa tcatatgatc gttttatatt gaatgaagaa
    12721 ccaatctcca cgatcggaaa gacaagcctg atcgaatact tcacgtatga attactgaaa
    12781 ccgttgaatg atattggtct ggaacggtca aattcagctt atgacacatg gaaaacaaga
    12841 catttttcga tcaattatac gttgaatgga caaaacgaat tagtcttttc ctttgtatta
    12901 ccgaccatcg atcaacgata ccaagtggaa tcgatgagtc tcctaaacgt atcaccagag
    12961 acaatggaaa tcaatgtcca agatgaccgg gtgctttctt tgatccgaca ctggtcagtg
    13021 gatcgagtat tctctgcagg gcagatcacg atattcaatc acaaattgaa tcaattatta
    13081 gcccatgcaa gaacacttgg ttttaccgtt aatcagacat tactagacaa tacgaagcca
    13141 cttcgtttga atcttcagag cgaatttgaa ttgacgaatg aagtattgga tgatattttt
    13201 atcgtggcaa tgaaaaatcc atcctacgat atggaaaaga tcaacgaaac tacttatcaa
    13261 gtacgattag ataaaggaca gtctttaacg ataacaacaa acgaaaaaga tcaaacagtc
    13321 ttgtcgatct catctggaga ctacccacga tctgtcattg atttctttat caattacgaa
    13381 ttcttggtcc cattaatggt gagaaagtcc tgattggata agttggatta ccagatgtca
    13441 ttcagataat tctgttccaa aggacttgcg gcagttatcc agaataattg tcacaagtct
    13501 tttctgtatg tactaaatgg acgtgaataa cttgctgatg aaggagggat ttcattagga
    13561 atgtccacaa ataaaatagc acagcttagc ttattgtctg ctgcttgtgt agcaggtagg
    13621 atcgcttttc aatttattcc gaatttccag ccaatgactg ctattttttt atttatagta
    13681 ctttacttaa gcttaaaaga tgcgttaatt gtcatgagtc tttctattgc gatcagcagt
    13741 ttttattttg gtgttggacc atgggtcatc ggccaatgga tcagctatac tgttgttctg
    13801 agtttgtttt caatcgtctg cttgagaaaa acagtgcaaa ggaatctatg gttaaaggga
    13861 gtctgttttt ttctggcggg catggtttac ggaataggta tgaccgtttt tgatacgctg
    13921 ttatatcgtt tgcctcaacc ttggatctat taccttcaag gggtgtcgtt tgatttgatg
    13981 cacagtatag gcaatgttgt gttctttctt gtttttctgc cagtcgtcaa acgtttttac
    14041 aatcattcag gaggaaaaaa tgaaaaaaat aatttatagt ttgcttgcaa tgagtgtcag
    14101 tatactcatt ttgacaagtt gcagtacaac ctatacacaa tcggcttcca accaaaagga
    14161 ggaagtaaaa acatcgcaaa aagcaacaat cacgttacaa gaaaatggtc aaaacttttc
    14221 ggaaaaagaa gtggtcttca aaaaaggcga tgatttactg agtattttga aacgaaattt
    14281 tgaagtcaaa gaagataacg gcttcatcac ttcgcttgaa gggcactcac aggatgagaa
    14341 aaatcaactc tattggatgt ttactgtgga cgggaaaagt gctactactg gtgccaaaga
    14401 gatcaaattg aatgatggac aatcggtcat tttcaatttg tctaaactat aagtttcata
    14461 aaaaactgtt aaagggcgaa tattgccata attcagtgag ttcagttgat gaaagaagga
    14521 aaataacatg gaaaatttta aaaaaagcgg aatgttttcc gtaattgctg ctctaggggc
    14581 taatattcta gttgctatca gcaaatttgt cggctatgca atcagtggca gtgccgccat
    14641 gctaaacgaa agcatccaca gtatcgttga ttgcagcaat cagattttgc tgttattcgg
    14701 tgataaacaa gccaataaag gacagagcga gttgcatcaa tttggtgaag gacgagcaaa
    14761 atactttttc agtacaatcg tcgcaatgat gcttttcttc ggcggtgggg cacttggggt
    14821 gatggaagct tttgaaaagt tgacgcatcc atcccatgaa gtgggtaatc cgatcattgt
    14881 tatagcaata ctgttatttg gtttggtaat cgaaggaagt tcgttacgcg tggcaatgaa
    14941 agaaatcaaa gaattgaata ctgaaaaact ttccacgatg cgctttttgc gcgaaagtcg
    15001 acacagtgaa atactgatta tttttacgga agacttgtgt gctgtaatcg gtttggtctt
    15061 ggctttagca ggaacaatat tgacttatgc aaccgggatt gctgcatttg atgcgatcag
    15121 cggattgctg atcggtttct tgctgatggg agccgccatt ttcctagcaa aagaatttta
    15181 tagtctgatc atcggtgaaa gcgtgacagc aagcgattta tcaaaaatca agttagcttt
    15241 tgaacgtcct gagatcagtc ggctgatcga tataaaaaca gttcatttaa gtccaacgga
    15301 gattttagtt gcagcaaaag cagatatcgt tggtaacaaa gagaatgaag cttactcggt
    15361 catcaatgat attgaaaaaa atatgcgtca gtctcttcct gacaagaaga tgtatatata
    15421 cattgaaaca gataaatatg acccaaatta ttctcgtaaa taaaacgttt attcatgaat
    15481 tgaagcaaaa atgagaaaaa tataatcttg tttttaaata aaacggctac aatttagcga
    15541 atttatgtta caatgagtcc gctgtttatt tgttgtaatt aaaggagata ttttaatgaa
    15601 caaaaagaaa agtgcgccat ggcttcaagc tttgcatgca gtgatgccac tttgtataag
    15661 ctatatacca gtaggattag cttgcggagt attgttgcaa aaagttggat ttgatcctct
    15721 cttatcagga ctgttatcga ttctggtctt ttccggtggc gcgcagtttt tagttgcatc
    15781 tatgcttacg acgcaagcat catttgcaac gacattattg atggttttct ttttagaatt
    15841 acgctataca ttattaggag caagtcttgc cggttttatg aaaaaagaaa agcggacgtt
    15901 tttggctgtc ttttctcaat cactgaatga tgaaaactat gcggtgaact acttaaagtt
    15961 ttcaactgac ccttcctgga ataaaagaaa ggctttatat gtcaattggt tctcaatgac
    16021 tgcatgggcc ggtagcaata tgatcggtaa ttttttcggc tcggtgattc gagtagatgc
    16081 tgaccttgtc cattttgctt tgacggccat gttcattttc atgtttatca tgcagatgaa
    16141 aagcgcacta ctgatcttta caggtttatt ctcaggcgta ttaggggtcg tattcatggt
    16201 attattccaa aatactttcg gattgattgc agcaacagtc attgcttcat ttgccggatt
    16261 tatgctggag aaggcattca aaaaagaaaa agctaagaaa ctaagagacc gcggtaaaga
    16321 tgtcaatgag ccgctcttca acttcccgga acaggaggcc aatcaccatg actaaactat
    16381 atttgctact tttagttgct tgtctgttca tcgtggcata tatcccgcgc ttgttcccga
    16441 tgctttattt tactcatcga aaagtgccat cctggttcag tgactggatg aaatatgtac
    16501 cggtggcatt atttgcggcg ttagctttta aagatgtttt cattactcat gaacatctgg
    16561 atattgcttg gaatatcaaa atagctgcaa tgattctagt agcaggcgtg gcttacaaaa
    16621 cacgttcgat ggcgttgtca gttttgacag gacttgcgtc tgtctttttg ctgtctatgc
    16681 tttaaactgt tttattttat taaaaacaga gattgcgaca taagttgttc aaactgatct
    16741 atccgaactg catttgcaac agtcaattct ggatgcaagt ttattcgtag ggataaaaaa
    16801 gattagtgtt ggcttatgat gcaagctctt ttttatttta ggaattagag agggatttgt
    16861 ccttctatga ttttttgcta taatagggaa gatgtttatt ttatgaaaga atagttgagg
    16921 gaatttaaat gaataaaaaa ttgatgcaag gaacattttg gctaaccttt gctaatcttc
    16981 tatgtaaagt tttaggggta gtctatttga ttccctggct aagtatgatg gggaacaatc
    17041 aagatggtat gcttgctacg acattgtata atgttggcta tcttccttat ggattgttcc
    17101 tgatgcttgg tacagtcgga tttccaaatg cgattgcaaa aaaagtagct gtagccacaa
    17161 aagaaggaga taacgctgct tgtcggctga tttttagaag tacgatcaat atcatgtttg
    17221 tcatcggtat cgcctctgct gcactgatgt atttatttgc tcctttacta gccggaatca
    17281 gtccgattgc taacgttaat aatgggatac tagctatcag aagtttatgc ccatctttga
    17341 ttgctatccc aattcttagt gcaatgagag gatatttcca aggaaaaaat gatctgcggc
    17401 cttatggaac ttctttgatt atcgaacagg ctgtgcgagt gatcgttatc ttggctggta
    17461 cttattactt gcgcgtattg actgacggaa ccatattaga agctgtattg atcagtacag
    17521 tcgcttcttt cttcggtggg ttagctgcga ttatccatat gtatatcgtc ggacgcagaa
    17581 aagacttctt cgaacttaaa gattttttga tttccagccg ttatttcgaa agagaaaacc
    17641 gatctgcctc tgtctctatc attcgggaaa cactaccgtt tatttttgtc ggctcagtca
    17701 ttactatcgt tcaaatgatc gaccaagtaa cgatgaaacc actgcttcac tttttccgtc
    17761 cagagatcgc agatcagcaa ctggaattct tattcagccg tgcttctgtc aatccaaata
    17821 aattgacatt gatcctgatc tccatggtag gaacagtagc tataagcagc ttgccaatat
    17881 taagcacgat gaagaaaaaa gatcggatac aaatcgaaaa aacagtagga gatagttttt
    17941 cgattgcgtt attgattctt cttccttcat tgactggaat gtctttatta gctggaccac
    18001 tatacacatt gttttttggc tatgatcctg aaagtgtcgg atatttccaa atggctttgc
    18061 tagcttcttt gtttttctct ttattcacga tcctatctac gatgcttcaa tctttgaatc
    18121 accatcgttt tgcgatccga ttaactgtag aagcaattat cctgaaagtc atcttccaag
    18181 taatcggatt aggattagtt ggcggatacg ggatgagctt atcaaatggc ttagcattcg
    18241 gtattgtctt tgttcgcggc tatcacttta tgtgcaaaga ataccgaata gcacctttag
    18301 caaagatcag caattttttc ttaaaaactt tccgctccac cctgattatg cttgcagtat
    18361 gttttgccgt tttctttttg ctgaatcagc gattgtcaat ggaatcaaaa agctatgcaa
    18421 tgatttattg tgtggctgta ggaagtattg gcggcttagt atttttattt tctcaattcg
    18481 gaaaaaatgg gtttcgaatg ttgaaaaatg ttaaacatca tagcagaagc aaagaatagg
    18541 ttaactaatc gttttttgct gtcacttgaa aaacggcaac tcgtatgatt acgcttgtcg
    18601 tttttctatt ttccaatttt agtttcatat ctaatcttac ttatgatata gttataaggt
    18661 ctggaaaaat tctttgaaac gaggaaatta aacacatgaa acttgcagta gttacagata
    18721 gttcggcata tctgccggaa cgtatcaaga accatcccga cttattcgtt attccaatcc
    18781 ctttgattat ggatgggaaa atttataacg aaggaatcga tatcgaagcg gatgaatatt
    18841 atggactttt aaaaaacagt aaagattttc ctactacttc acaacctgtc gtaggggaag
    18901 ttttgggttt atatgaggaa ttgaaagaga aagggtatga cacgatttta agtatccatc
    18961 tttcttcagg gatttctggc tttgtcaaca cattatttgc gatgaaagat gacatcagcg
    19021 gagtaacggt cattccttat gactctaaga tcactagtat gccgatggga cacatggtag
    19081 aagctgcctt agacttaaat gatgaaggtc agtcccttga tgaaatactt gcacatatcg
    19141 atcgaatccg agataatacc tatgcttatc tgattgtaga tgatttgaac aatttagtac
    19201 gaggaggacg tttgacaaat ggtgcagcgc tgattggcag cttattaaaa atcaaaccga
    19261 tcctgacatt ttctgaaggg aaaattgttt tattcgaaaa aatccgttca agtaaaaaag
    19321 catttgctcg agctgaagaa attattggta gaagaaataa cgaaatacaa ccgccagtca
    19381 aactttacgt gatacatgca aataatttag aagtagcgat tgcggagaaa gagaaattgc
    19441 agaaaaaata tccaaatgca gaaatcgaaa ttggtcattt cggaccagtc attggtacac
    19501 atttaggtga aaaagccatc ggcattgcca tttcagcgca ataattcctt ttattcaaaa
    19561 aacataccaa aaata
//
LOCUS       DARDHA010000038        19475 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000038 DARDHA010000000
VERSION     DARDHA010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.038"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(21..995)
                     /locus_tag="SDA10_001108"
     CDS             complement(21..995)
                     /locus_tag="SDA10_001108"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEG4240034.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="SDA10_001109"
     CDS             complement(1075..1950)
                     /locus_tag="SDA10_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HEG4240035.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="SDA10_001110"
     CDS             2080..3987
                     /locus_tag="SDA10_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HEG4240036.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="SDA10_001111"
     CDS             complement(4528..7806)
                     /locus_tag="SDA10_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEG4240037.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8077..8622)
                     /locus_tag="SDA10_001112"
     CDS             complement(8077..8622)
                     /locus_tag="SDA10_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG4240038.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8634..9326)
                     /locus_tag="SDA10_001113"
     CDS             complement(8634..9326)
                     /locus_tag="SDA10_001113"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEG4240039.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9345..9629)
                     /locus_tag="SDA10_001114"
     CDS             complement(9345..9629)
                     /locus_tag="SDA10_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240040.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9642..10205)
                     /locus_tag="SDA10_001115"
     CDS             complement(9642..10205)
                     /locus_tag="SDA10_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG4240041.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10433..11095
                     /locus_tag="SDA10_001116"
     CDS             10433..11095
                     /locus_tag="SDA10_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEG4240042.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11219..12415
                     /locus_tag="SDA10_001117"
     CDS             11219..12415
                     /locus_tag="SDA10_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEG4240043.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12516..13892)
                     /locus_tag="SDA10_001118"
     CDS             complement(12516..13892)
                     /locus_tag="SDA10_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4240044.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13929..14795)
                     /locus_tag="SDA10_001119"
     CDS             complement(13929..14795)
                     /locus_tag="SDA10_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HEG4240045.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(14884..15909)
                     /locus_tag="SDA10_001120"
     CDS             complement(14884..15909)
                     /locus_tag="SDA10_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG4240046.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15927..16601)
                     /locus_tag="SDA10_001121"
     CDS             complement(15927..16601)
                     /locus_tag="SDA10_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4240047.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16785..17678)
                     /locus_tag="SDA10_001122"
     CDS             complement(16785..17678)
                     /locus_tag="SDA10_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG4240048.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17752..19449)
                     /locus_tag="SDA10_001123"
     CDS             complement(17752..19449)
                     /locus_tag="SDA10_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG4240049.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLAKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         5683 a         4189 c         3446 g         6157 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt
     7861 tatttttact gtcaataaaa aacaaaatta taataagtgt attttataaa aagaaaaaat
     7921 ataacttaag ttgttgataa taaagagatt tattaacgta aaaagaataa gtttttttaa
     7981 aataacgtat aaaaaagagc ataaaacaaa gtcgtttgtt ttatgctctt cttttattat
     8041 tttgtgcttt ttattttatc aaatcacttt gtttttttat aaaatttctg cgcctagtgt
     8101 ggactcaaaa tgtctcaatg cccattcatg tccaacggga tcgaaacttg ccacggcatc
     8161 tttgaaaata tggagtttgt atcctagatt atacgcatcg actgctgtat gcaacacaca
     8221 gatatctgta catacgccgg tcaaataaat atctgtgatc tggcgttctc gcaaacggat
     8281 gtctagatct gttccactaa aagcggaata atgccgtttg tctatccaat aaacattttc
     8341 ctcttgctca tagtcttggt ataaactttt taaagagccg tacaattccc gtccacccgt
     8401 tcctatcaca ttatgcggag gaaataagcg gttctccgga tgaaaggaat cttttggatc
     8461 atgcgcatca atggcaaaaa cgacaaaatc cttttgatca ataaacttct ttgtatgagc
     8521 agtcagcttt ttttcaatta cttgtcctgc tgtcccagta gtcagctttc catcctctgc
     8581 aacaaaatca tatgtgtagt caatcgagat caatgcgttc atcttagtcc tccttatacg
     8641 agatgtttga caaattcgat caccatttct gctgatcgtt tgcctgcgtc ttcgataaat
     8701 tcgtcaaagc tttgtgtcgc cgagtgatct gctgtgtcgc tcattgcacg tacgatcaaa
     8761 aatgggatat tgaactgacg agctgtttgt ccaacagcgg ctccttccat ttcgcaagct
     8821 aatgcttcag ggaaattcgt taagatttct ttgattttat ctggcgagtc gacaaatgta
     8881 tcacctgtaa cgatcaatcc ttcttttgcg ttcaaattcg ttgcttttgc tgctttaacc
     8941 atttcactgc gcaaatatgt gcttgcttca taatatagag gcattcctgg taattgtcct
     9001 ggtttgtagc caaatcctgt tgcatctgca tcaaaataag ctactttgtc tgatataacg
     9061 atgtcaccta cttgtaagcc ttcaccgatt ccgccagctg aacctgtatt gatcaccatg
     9121 ttcacgccgt attgttggat caacagactt gtggtaatag atgctaagac ttttcctata
     9181 ccggaacgta cgacgatcac ttcgtggtta cctaatgaac ctgaaataaa taaagcgcct
     9241 gcgcgttccc atgataatgg ttcgcttagg ttctctctca aaatttttac ttcttcttcc
     9301 attgcgccga taataccgat tttcatcttt ttaattcctt ctttctatat aaatgcgatt
     9361 gctaaaaaaa ccacacataa cattaggata acaataataa gagatttcat caaaaaagag
     9421 ttccatttgg tcgtcttttc ccgttcgctg atccttgttt tcgtgattgg tttattcttt
     9481 ttgctttctt tacgataaaa gctggcaatc tttttttctt cttgctgata agctgtttct
     9541 gcttttcgct gtttttttaa tgactcgctt gcttgtgctt gttgtcgctt gcgaagttcg
     9601 cttcgcgtaa taagcggtcc tttggccata agccgatccc tctactttcc tttggtcaat
     9661 agttcccagt attgaatcgc ataaatcgtc tttgcatcac aaatcaactg atctttcatc
     9721 gcttgttgcg cctcttcaag agttagatga tacaactcca gtacttcgtc ttcatcttgt
     9781 gctaaaggat tctcgacctt ctctacccct tgtgcatgat aaatatgaag gacttcattt
     9841 gcaaaacctg gtgaaagata catcgaagta agatggctca gcgacttcgc acgatagcca
     9901 gtttcttctt ctaattctct agctgctgtc atttcgggat tttgtccttc tcctggatcg
     9961 atttttccag ctggaatctc caaaatcact ttttctaaag gttttcgaaa ttgtttgaca
    10021 agaagcagac gatcttgttc gtcaaaagca atgatcccga caccaccagg atgaaatacc
    10081 agttctctct tcgaagttcc tccatctggc aaacgaactt cgtctacagc aacatcaata
    10141 atttttccgc tatagatttc tttgcgactg attgtttttt cttcaaattg ctcattgttc
    10201 agcatatctt ctcctgcttt ctttgtaatc ataactgctt ttttttcatt tttcaatttt
    10261 tctcaaaaac tccttattag tttaccatag ataaacctta gattttctct aaatatagaa
    10321 aatccttctt tagtccgata ctctttctag tagtccgtat gctaaaataa gtatgtaatc
    10381 gaataacgaa aagaattcaa tactcaacga taaatgacat ggagggttta ttatgataaa
    10441 acgaaattgg ccgtggcttc tagcattggt cttgctattt ctctttgtcc gaaacaatag
    10501 tttttctttt gttctaggac ttttattgat aggtgtagtt ttagttttgt ggggactctt
    10561 tggttcgcgc aggaaaaagg gaaaacaaga agaaattcct gcactgactg acgaacttga
    10621 aactcactat gaaaaaagcg gtatgacagc gaatgaaatt tcattttttc gacaaacaat
    10681 gaatcaaaca aaaaatgaga tcaatcaatt gcaaatgaat atgcagcaga cagccaaact
    10741 taaagcaatc gatctccgcc atgatccagt caaagcagcg aaagctttat tcaaagaatt
    10801 ggtgaaagaa ccaaaccgac tgcatgaagc aagtcagttt ctttatacgc atctgccgaa
    10861 tctagttgat ttgacaaata agtataatga gatcaatgac catgagatca aaaacaaaca
    10921 gacctatgaa aaactagaag aaagcgcgca gatcatcgac cagctgtcag ctttaatcgc
    10981 acaagattat caaaaattcg ttgctgagga tctagatgat atcgacgtag agatatctgt
    11041 tgcgaaacag agcttaaagc gtgataataa atatgaacca gaacaaaagg attaactctt
    11101 ttgttcataa aagaagactc ggaacattca gatgatgtct ggctgtttcg agtacttttt
    11161 ctaaagtaga gacattcatg taagaatcga gaacagattc aacaaggagg aattttttat
    11221 ggatcataaa ccgactgaaa caaaagatgt tacaccagtt aacgatacac ttgaagattt
    11281 attgaacaac ccgttttcaa ctccaacaga tacgctgacc acttcacaac aagcggagat
    11341 tgatcagctc caagagcaac agacagctgc tcgtttaatt gacaaactac cggcggaacg
    11401 ccaagaacaa gctaaacagc tagcagcaaa aatcgatgct aatgatgcac agtctgtcat
    11461 cagctacgga tcggctgccc aagcaaaatt aagtgaattc tcacaatcga tgttgaatca
    11521 tgtgcaagca caagatattg gtcctgtagg agattcctta actgaactga tgtatcgtct
    11581 ccaagaagcc aatcctgacg aactgcgtgc cggagaaggg aacatcttcc agcgtgtctt
    11641 cgggaaggtc aaacaatcga tctatgaagt gacagcaaaa tatcaaaaga ttggtgcaca
    11701 aattgacaag atttcagtga aattagataa agaaaaagac ggtctgctaa aagataatct
    11761 gatgttagaa cagctttacc agaaaaacaa agactacttt gatgcactga atatttatat
    11821 cgctgctgga gaattgaaaa tggaagaact tcaaacaacg atcattccag aagcgatgaa
    11881 aagagcagaa gaaactggcg atcagatgga tgttcagatt gccaatgatt acacgcaatt
    11941 cctagatcgt ctggataaac gtacgcatga tcttcgctta gctagacaga tcacgatcca
    12001 gcaagcacca caaatccgtt tgatccaaaa cacaaatcag gccttggctg aaaagatcca
    12061 agcatctatc gctacagcca ttccgctgtg gaaaaaccaa gtcgtgatcg cattgacctt
    12121 gcttcgtcaa aaagatgcag taactgctca acgccaagtc tctgaaacaa caaacgactt
    12181 attgaagaaa aattcagaaa tgctgaaaat ctcagccatc gaaacagcga aagaaaatga
    12241 acgtggaatc gtggatatcg agacccttca aaccactcaa aatgatttga tcgaaacaat
    12301 ccaagagaca ttgcgtatcc aaaaagaagg aaaagaaaaa cgtcgtcatg cagaagtaga
    12361 gcttggacac atggaagaag atctgaaaca aaaactgttg gatttaaccc aatagatcac
    12421 ctttcgcttg ttactaaaac aaacgcccaa caacagttta ttacaactgt tgatgggcgt
    12481 ttttcttatc tttcttctaa cttctccatt ggagtctatt tctcattcat cttgctgtgt
    12541 ccatagccat agccaaaata aatgatcagt ccaataacca ttacgatacc gaacacgatc
    12601 catgtcacag tttgcagacg aagcatcaat acaatacaag tcaagatgga gacgattggc
    12661 aatagcggaa cgaatggcac tttgaattct ccagcttttg gtttgccaaa catttttctc
    12721 aacttcagta agcctagact aacaagcatc aaataggcga gcgccatgat attcgttagt
    12781 tccgctaata cattcaatgg gaaaaatccg gcaaagaata cagctgccac accagcggca
    12841 taagtcgctt ttttcggtgt acgatgcttt tgatcgattt ctgtcataaa cttaggaagt
    12901 aagccatcct tgctgacagc aaacagtaaa cgagcaagtg aatacatcat cgagatcgtt
    12961 accgtcagta gcgttaaaat tgcccctaca gcgatcaccg cgcccactgt tccatgtcct
    13021 acatacctca ttgcaaaagc cactgggtca ttgactccta gttcagtata cggaacgatt
    13081 cctgtcaaga tcagcgtcac gatgatgtaa aggacagtag caatcagtat cgaaccaatg
    13141 attcctcttg gcacatcttt ttgcgggttt ttgacttcct cggctgccat tgcaacggca
    13201 tcaaatccca agaaagcaaa aaacacaaga gctgcaccat ctagcacacc ggaaaaacca
    13261 aaaggcatga atggctgcca gttatcaggt ttaacgaaaa atattcctac aatgataaac
    13321 aacgcgatga taccaaattt gacatatacc atggcattat tcaaacgtaa tgctttcttg
    13381 gcttcaagcg acacgatgta agtgacgaat atcactacga gcaccgcaat caagtcaatg
    13441 tatgtaccat tttccggatt gtacccgcca gttaatgctt gtggaagttc ggcccctaaa
    13501 cttttcaaaa aaccttgcat atagccagac catccggaag caacagagga aaccgctagt
    13561 aaaaattccc cgatcaacaa ccaccctgtc agccaggcag caaattcacc aaatacaaca
    13621 tacagatagg catatggccc accaatcact ggcactcgcg aagcaaattc tgcgaaactt
    13681 agtccagaca aaatgacaac gattgcagct aagacaaatg aaagtgacag tgctggacca
    13741 gcattttgat tggccgcaac ccctgtcacc acaaatattc ccgtaccgat gatcgcacca
    13801 attccaagca tgatcaaatc agacgttttt agttcttttt tcatctcact cgtatgattg
    13861 agattgatct ctttcttacg aaacaaatcc attgaagttc ctccccattt taggttggaa
    13921 aacagcgatt aaactgtttt catataccct aaaaggatac cacctaaaat gactaaaagg
    13981 caaccaacaa taacataaat gagctctttt ttcgtttttc tttcgccaag gatgaaaatc
    14041 ccacctaaag tagagataat gatccccatt tgtgaaagtg agaagccaac agctaaacca
    14101 agtgatttga ctgttagaag catgcagatg tttcctaagc cccataataa tccacagatc
    14161 atgtttttcc acacaaactg atcgacttta actttacgga aagcaaagaa gcttgcgcct
    14221 aagatcattc cgatactttg aggtaagata attgacatcg catccaaatt cgcccagttg
    14281 acgatgatgg tatatacacc ataaccaatc gtagagaaga tcaatgctct aaaaccctta
    14341 ccaaaatcaa gcattttatt atctgtatct atttttcctt cgtcatcttg tcgggcagtc
    14401 aaatacgcac cgcttacaag aagagctaaa gcaataatac cgtatacata atctttcgtt
    14461 tgtgtccatt catggaagaa aacggctccc gcaatcgtat tcaaaattaa ttgaaaacca
    14521 gtagacaatg gtagccctac agaaacaccc atgtacttca ttccatggaa ctgtccattt
    14581 tgtccaacac tccaagctaa gccggataaa aatccgatca agaaaatcca aggggtgatt
    14641 acaggactaa caacgaaaaa gacaactaaa gaaaacagaa atgcaccgat cgtcattcct
    14701 agtgtttgtt gattggcatc gccaccaatt ttaccactta ctaaaccaat actaccccat
    14761 gccaacatag ggactaatgc tattaaaatt tccataacca atctcctttt atttcacatg
    14821 ataagaaact tatcagcgaa aacgttaaca acacaagcac tttttttcgt ttctaacgat
    14881 tttctaacgt ttttcattat aaaaatttaa gattgggaaa aataaagtca ttgttgtccc
    14941 ttttccttca gttgattcag ccgtcaaatg gatacctaat ttttcggcca gattttttgc
    15001 taaataaagt cctaatcctg ttgaatgtgt tttgctaagt cgaccatttc tcccagtaaa
    15061 tcccttatca aagatccgtt tcaagtcttc cgctgggata ccgatccctg aatctttaac
    15121 tgaaaggctg actccttgcg cttccttttc tatgctcaca atgatgtttc cgtgatccgg
    15181 cgtatatttg atggcattac taagcaattg gttgaagata aaagcgatcc atttagcatc
    15241 ggtcaaaaca gtatgatcct caccaacgat ctcgtagcga atattttttt ggataaaata
    15301 atttgcctga ctgcgtatca caggctgaat gattcctttt agtccatatt cttggatgag
    15361 gtagtcccgc gagaaactgt cgagtcttgc ataatacaaa acttgttcta cgtattcatc
    15421 gatttttgat aattcatttt ctagtaatgt atacttttga tcgtcaatat cgaactcgat
    15481 cgaacgaact agcaatttag aagcggaaag tggcactttg atttcatgga cccatgagtc
    15541 aatataatct ttttggtctt gttgattact gattgctgat tgcattactt cttggtgttc
    15601 acggatcagc tgattgatat attcttctac cagtttttct tcttctgtcc tcccaccact
    15661 caaataattt tgcaaagggg aagttttttg tatgtcggct aattttcccc accatcttct
    15721 cttgctcaag taaccaccca gcagataaaa aatcagaaag acgccctcaa tcaaagctaa
    15781 ataagcaatt gtgctcaaat caactttgat gtttggcgcc agccacatca caaagatcgt
    15841 caatgcaata aaaaacagcc atcccaagat caatgtccac tgatctttca aatatttaaa
    15901 aaaattcatc ttaaccgctc atcctttcag ggaacgatat atccctgtcc tacttttgtt
    15961 tcgatatatc cttggatacc tgcctgttcg atttttcggc gtaaccgatt gatgttgact
    16021 gtcaatgtat tgtcatctac aaatcgttcg tcatcccaca gtgctcgcag caatttttcc
    16081 cgacttaaga tctttccatt gtgcttcatc aaaataaata atagacgata ttcatttttg
    16141 ctcaaatcaa tgatctctcc gttgatttcc atacttccgt tgtcaacatt caacgtaatg
    16201 ccattgtggc tcatcatttc actagataat tctgtgtagt tgtaagaacg gcgaagtaat
    16261 gcattgattt ttgcaatcag tacatcgatt tggaaatgtt ttgtcacaaa atcatctgcc
    16321 cccatgttca tagccatgat catatccata ttcgtattcc tgctggagat aaaaatgatc
    16381 ggtactttcg atacttcacg tattttttga caccaataat agccatccaa aacgggaaga
    16441 ttaatatcta gtaatacaag ctgtggttct tctctttcaa agtctgagaa cacttgattg
    16501 aaatcggtcg ttccaaaggt ttcaaattgc catttctcaa gttcctccat gatcaattgt
    16561 cgaatgatct cttcgtcttc gactaccatg atttttgcca taaaaaatcc ctccatcttt
    16621 cttcttgcta ctacaatatc aaagaagccc ttgtgaaaca agttggctta cctggaaaat
    16681 gtcattttag caaacgttaa aaaaaactgg aaatatgcga tttaatcgcg tttgtctctt
    16741 ccaagcagct taaatttatt atgttattct taataattgg tctattatct gttatttttc
    16801 tttcgttgtt tttgtctttt aaattttcga taacaccaat aaccgaaaag cgttagcact
    16861 agcaatatac ctagaaggag tataagtaat cgataaagat ggtatttttt tgtctgttct
    16921 atctttgaag agacttcttc tgtaaaggga actcgtcttc ctgtcactag taatcggtga
    16981 gtgttcacgg tgtaaggtgt acaagtgata agtgtgacta gatcctgctt ttctctaatt
    17041 gctaatgtat ttgtttttgt tggaaggaca acaattttct caacgacttc ataagcgagc
    17101 atccgaccat acacttcaag atagaatcgg tcatcttgct ccatttcttc aagatccgta
    17161 aagagttttt tctcagcaat accgctatgg gcggtaatca cggaatgtgt actctctcct
    17221 ccagttggaa aagaggtgcc ttgcaggact gtcgctcctc tatctaacaa aagatcattc
    17281 gtttcatcaa ataaaggcaa gctaacattg attttcggaa tatagatggc tccgatcata
    17341 tgttgttcgt aatattcttt tcctggattt tggacatttc taaccgcttg ctcaaaagga
    17401 tctttgactt gtcccattcc tgggattact gatgtatgag caagcgtctg atttttcttt
    17461 tcatgcactg ctaattgttg ctcatgtttt tcttctttat cgcgttcaag ctgcttttga
    17521 taattctcga tccttctttg atccaaataa ttgctgagag catctgctaa aaatggatat
    17581 gtaaaaactg cagctccggc aagaaacatc actgccataa ataattgtaa taaaagactg
    17641 atttgggttt gaggtctatt acctattttc tttttcattt tcttcacctt cttatttaaa
    17701 aaaactgcag caacctttat aaaaccgatt gctgcagctt atttttgttg cttatacttc
    17761 agacttcatt ttagatttac ggtaccagct gtacgctcca atcatcaaac ttattccgat
    17821 tagtaagaat gccaagagcc cgttcccacc agtagcagga agtgacccgc gtttcatatt
    17881 cggtactctt tctggccctg gattttctac tcctacgatc tgaatttctt cattatagct
    17941 tccataagag atgataaatt cagtaaaggc tgcctcacct gttggaagta cataaccttc
    18001 tggcgccatg gtctctttca tttgataggt tccataatct aacccttgaa cctcaaagta
    18061 tccatttcca tctgcgacaa attctgttgc ttgtccgctt gttgcccact caattctatc
    18121 tgctcctgat tcataaacag ctaaaggcga ctccccttta taaaatattg cgtattcaga
    18181 acggtcttca ttccacaatt cgaatcttgc atctttaagc aattcactgc ttgatgcatc
    18241 atatttttca aactttcttc cacctgttgt gactttctct ggcggctcta ctccttcttc
    18301 ataatcaaat tcatcatttc gtccgatttc aataaccgct gtgttgttat ggaaatcatc
    18361 cgcttctagt tctgtggaaa ccttcaaatt atacgtgatc gttaaatctc ctccacgtgc
    18421 atctgctaat ctacctaaag tatctacttt ggctactcca ttttcttctg tcaaaatcgt
    18481 cacgacaaac ccgttgctta ctacttctac tatataatct tgtccttttg ttaaaatcgt
    18541 ctcgccggct cgcacttcta tttcctgatc ataataagtt agaccaactg taggcgtatc
    18601 tgtgattttg taatttttga taacagcgct tccgttttca aggacagaat cgatgaaata
    18661 aggaataggt gctgtgatcg tgtaagaagc cgtttttcct ctttctagat caatataaga
    18721 aatcgtttct tgttctccag cttcattaat aatcgttact tgtctaaggt cgctttcctc
    18781 tacattcatc tgtttatctg cttctctgat ttcgtttttc ggatacaaat gcacatctgt
    18841 attgtatgtg tctttccatg tagaaccatc ccacacgcca tccaccatat caggcatcat
    18901 cactggcatc ataacaacca ttggagacgc gattctggaa atatgagctg gcgaataagt
    18961 ttctaagaaa agatagactt tatctctaga agaaggtgct tcttccgtca cttggacggt
    19021 agagaatacc gcttctccag cttcattcgt tgtttgtttg tcaatacttg tcagtcgata
    19081 ttttaaaaac caactttctg tgtgccgttt ttgaatccat tcaatcgctt cggcagaatt
    19141 aagaccgtct tctcgttccg gtgttaaagg attatctcga atcaaatcgt aatatacttc
    19201 cgttacgtca aacaaatcaa aatcgactcc tggaatattt tcttcaccaa aatccgtaga
    19261 aaccaatccg ctattcggtc gttcttccgg aacagatgag aaaccttttt tgtgtaatgt
    19321 aatcgtcact tcacttggtt tttgtgcgac ttcttcggct ttggctagtt ttccgttccc
    19381 gaaaatccct attcccgcag taacgagtaa cagtacactc attcccgtga caatcatttt
    19441 tttattcatt tttttctccc cgttcttttt ttatt
//
LOCUS       DARDHA010000039        19036 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000039 DARDHA010000000
VERSION     DARDHA010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.039"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            22..576
                     /locus_tag="SDA10_001124"
     CDS             22..576
                     /locus_tag="SDA10_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG4240050.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(749..1222)
                     /locus_tag="SDA10_001125"
     CDS             complement(749..1222)
                     /locus_tag="SDA10_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG4240051.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1234..1770
                     /locus_tag="SDA10_001126"
     CDS             1234..1770
                     /locus_tag="SDA10_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG4240052.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1810..2181
                     /locus_tag="SDA10_001127"
     CDS             1810..2181
                     /locus_tag="SDA10_001127"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG4240053.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2465..3736
                     /locus_tag="SDA10_001128"
     CDS             2465..3736
                     /locus_tag="SDA10_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG4240054.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            3981..5006
                     /locus_tag="SDA10_001129"
     CDS             3981..5006
                     /locus_tag="SDA10_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4240055.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5300..6409
                     /locus_tag="SDA10_001130"
     CDS             5300..6409
                     /locus_tag="SDA10_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEG4240056.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6492..7388
                     /locus_tag="SDA10_001131"
                     /pseudo
     CDS             6492..7388
                     /locus_tag="SDA10_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7404..8228
                     /locus_tag="SDA10_001132"
     CDS             7404..8228
                     /locus_tag="SDA10_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4240057.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8260..9570
                     /locus_tag="SDA10_001133"
     CDS             8260..9570
                     /locus_tag="SDA10_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEG4240058.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9718..10977
                     /locus_tag="SDA10_001134"
     CDS             9718..10977
                     /locus_tag="SDA10_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG4240059.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            10987..11859
                     /locus_tag="SDA10_001135"
     CDS             10987..11859
                     /locus_tag="SDA10_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4240060.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11871..12701
                     /locus_tag="SDA10_001136"
     CDS             11871..12701
                     /locus_tag="SDA10_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4240061.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12741..14924
                     /locus_tag="SDA10_001137"
     CDS             12741..14924
                     /locus_tag="SDA10_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEG4240062.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            14985..16205
                     /locus_tag="SDA10_001138"
     CDS             14985..16205
                     /locus_tag="SDA10_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HEG4240063.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16202..17662
                     /gene="gtfA"
                     /locus_tag="SDA10_001139"
     CDS             16202..17662
                     /gene="gtfA"
                     /locus_tag="SDA10_001139"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEG4240064.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17739..18845
                     /locus_tag="SDA10_001140"
                     /pseudo
     CDS             17739..18845
                     /locus_tag="SDA10_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         6459 a         2906 c         3714 g         5957 t
ORIGIN      
        1 tttttaagaa gggaaacaat agtgagctat aatgtacaac cgttaaggac aaatgaagag
       61 atacaagatt ttcagttttg gctaagaaga acaagtaatc cggaaagaga tagtttttta
      121 tttttgtttg gaatcaataa tgggttacgt atgtcagata ttataggatt aaaagttgga
      181 gatatacgag gcaaaagtaa gcctattatt gttgaacata aaacaggtaa gcgaaaaccg
      241 atttttatag ataatttgag agaagaaatt ttattatata ctgaaggtaa agaagaaaat
      301 gattggttat ttccgagtcg gcaacaaggt cgacatataa caagggatcg tgtctatcag
      361 atatatgcag atatagcaga aaaattagga cgagatgata taggaacaca tacattaaga
      421 aaaacttttg ggtatcacta ctacaagaaa actagagaca ttgctacctt aatgtttatt
      481 tttaatcata gctcgcaagc aattacaaaa aggtacattg gtataactga agatgaaata
      541 ggagcatcac ttcgtggatt taaactaggt gtataattta aaaattttta taaacagtaa
      601 agacaaagat taaaagctat ttttatctag aacgatttta tttcataaag ttcgcggaat
      661 aaaagctggg gttactggta cttggtatag gttcgtgtta tataaactaa gtaattaaat
      721 aacattttta ggttggaaaa ggatgaaatt aaaaaaagag aatcaatacg gtctcaaaaa
      781 cgcaaacgct gcggaataag aaaaatactg ataactcaac gtttagcaga ctatctaata
      841 gggatagtct gcttttttcg tttttgacca tacttttgac cttctgcaaa cttccttgct
      901 tttcctctgt agaactcatg aaatctgcaa aacgaattgc cgtttcctct gtctgctttt
      961 tcgcgatatg ggtgtatata tcatccatag tggttttaat atctgtatgt ccaagtcgaa
     1021 catacacttc tttgacagac gcaccagatt caaagagcag actacaatgg gtatgacgaa
     1081 aaccatgaag tgtgatatgt tttaagttat tctctttaat gatgattttc aaattgtgat
     1141 ttacatagtc taaataaagg ggcttatttt cttctgatga aaacaaaagt tgttcaccat
     1201 cttttatagg tttgattctc atttttagat attatgcccg cacgtattca tcacatttct
     1261 attgtgaatc gctttattcg accgacattt gacttttatc ataatatact cggtttaaaa
     1321 ttactaatga aaacaattaa tcaagacgat cataccatgt atcacctgtt tttttcagat
     1381 aatcaccacc gtgtgggaac tgaactgact ttttttgaag tacaagaagg gaacaaccag
     1441 ttttttggaa ccaatacgat tgaacgaacg atactaaaag ttccttccga agcatcattg
     1501 catttttggg aaatatattt tgaaacacaa ggcgtttgcc attatggaat cgaatcattt
     1561 tctggacgac ctatcttacg ttttgaaggg ccagatgcaa cacgattagc tttagttccc
     1621 ttacgcgaat ttgaagatat tgatgattat ttcccgtatg tcacagacca gatccctaca
     1681 gagcatgcca ttttaggaat tgatgccatt caactacgcg tacaatattc cgatgcgact
     1741 gaacgagagt tattgtcggt aggctggtag cacaaagaat acgtcagttt cttttcgggt
     1801 catcaacgag tgacaatctt agaaaatcaa gacactcagt tctatcaaga agttcagatt
     1861 attcaagatc gtgagaatcc agttgccatt gaagggattg gtagcgtcca tcatgttgcg
     1921 tttggcgtag aaaataaatc tgatttacaa cgcatcgaca aacaattaca agagcgcaat
     1981 tttataaatt caggaataaa agatcgtgaa ttttttgtat cactttatta tcgtgaccca
     2041 aatcaattat tgattgaaat tgctacaggg gaaggaaacc tagatgcaaa agcgtatgaa
     2101 aatcaaagtc cacgctttga agaaattcct ttatttttac cgcagtactt aaatttcatt
     2161 cgtgaacaag tggagcaata attagctaat caaatccatg acgaagagta agaggttgac
     2221 tcataagtca aaaaataaag aaaaacgcat caaatccatt ctaaagaatg ttgatttgat
     2281 gcggttccta tttttataaa ctatcatttt cgactaataa aactacttat gagtcagcct
     2341 ctcttttttt cttgattttt atacctcttt ctaggtaaac taaacatctg ccaaaaatac
     2401 cgtgtctcat tgaaacattc tcaactattt caaaataaag gggagtacca aatgaacgag
     2461 tatattgaaa ttatccacgc aagagaaaca acttaaaaat gtcagtctca aaattcccaa
     2521 aaataaaatt agtattttta ccagcgtatc tggttcagga aaatcatcga ttgtttttga
     2581 tactattacc caagaagctg gtcgtcaact gaatgacacc tatgatagtt ttacccgttt
     2641 atttttacct aaatacagtc gtccagaggt tgatgagatt gataatcttt ctacggcgat
     2701 tgtggtcaat caaaagaaga ttggtggcag tgcgcgctca acgttaggga cgattactaa
     2761 catcaatgcg ttatttcgtg tgttgttttc atgttttgca acacctagtt taggatatgc
     2821 aaatgcttat tcatttaatg atcctagtgg aatgtgttct gtctgtcagg gaatcggtaa
     2881 gacgattgca tttttaatag gattgcttta tttttcggct attgataatg ctgattttgt
     2941 attttctgat ccatttaccg cagaagtttt gtctggaatt gaggaacagg caaagaatca
     3001 tggttatttt gtattggtgc ataatgttaa ttcagcagaa gatgttaaaa ctattcagag
     3061 aaattggcgt tttgaggggt tcattgttgt tggtgtacgt gtaaaagaat ttttggaatt
     3121 aaataacgtt tttgatggtc ctgttgttta tattgatacg catctttcaa aagagacatt
     3181 aagagaactt gaacaacaaa tgaatcgttt atttgtaaat acaaatgatt accaagctag
     3241 tacattggca gtggaacatt tgattcatta tggtcatgag aaaatagcgt ttctttcttt
     3301 tgactacatt gtaggtgaac caagtgttat tcaagaacga catcaagctt atatcgatac
     3361 attaaagaaa tatcatttaa cctttaaatc gcattggata tataacaact ctgaatttga
     3421 gcgaatttat caagaattag ctgaatttac tgcaattgtt gttactggag attatttagc
     3481 ggctaaatta gtaaaatttt taaaaaataa gaataaacga gtgatggaac aattatcgat
     3541 tattggattt gatgatatta cttttgcaga attaatggat cctgcgttga caacaatacg
     3601 actaaatcca ggtaacaagg gggcgattgc ttttcaccaa ctgattggac tggccaaaca
     3661 agaagaaata aaaaatcaag tactactatt agagggagaa ttgatttctc gagactctgt
     3721 tcacaaaatc aaataaaaaa gctctgtgaa ataagtatag ttaacaataa aatatccgaa
     3781 cgatagagag aatgaactgt gatctagcag ttcctcgtcg caaattcact agaattagca
     3841 ttatttactc taaattgttc ggatattttt tcttgttttt aattatgatt acagtgccta
     3901 tttttaacga aatttgctta ttttcaaaat gtatgattat atgttagtaa acactttcaa
     3961 atatgaaaag aggagctatt atggtaggaa ttagggatgt tgcaaaacgg gcgggtgtgt
     4021 ccattgcaac agtatcgcgc gtgctaaatg aggatccgac actttcagta accgatgaaa
     4081 ccaaacgaaa gattgcagaa tcggttaatt tttatcatta tgtcaaaaaa agtcccgttg
     4141 caaaaaattt aacaaatatt gcattaataa cgacagtttc tgaagtagat gagttggaag
     4201 atccctattt tcgaacgatt cgcagaggga ttcaagtcga agcagaacaa agcaaagtca
     4261 atttgaagaa aattattcgt ttgtcagaaa caccgttgga gcaagacgat ttgctaaaat
     4321 gtcaaggtgt gttgattatt gggcaggtgc tatcttctgt gattgaagaa gtatcagcaa
     4381 ttaattcgaa tgtcgtagtg attgatgacc ctagtattga agctgaagta gatgcagttt
     4441 atactgattt aacaaaagca acgaatcagc atttagatcg attgtataaa aaaggacacc
     4501 gaaacattgc gtttatcgga ggaggacgtg ttcgtttaga tagccaagga aataaacacg
     4561 tgtcagatga tgattatcga aaaatagcct atgagacgtg gatgaaacaa aaaggattag
     4621 aaaaatatag tcaaatttat ttagaaggct ggaccacact tgacggaatg caggcagcag
     4681 atttactttt gaaaaaacat gctaaagata agttgccgac tgctatcatg gtaggaaatg
     4741 atcctattgc tgtgggggtt tatcgttcac ttcaaaaaaa tggtctacat attccagaag
     4801 atatttcgat tgtgagtttt gataatattg aagttgctga atttttaaca cctgcattaa
     4861 atacggtgaa tgtgaataca gaagaaattg gaaaactagc agttcgcatg gttaatgaac
     4921 gaataaaaga agttagaaat gtttcgatca aagtgattgt ttctaataat attattgtta
     4981 gagaaagtga aaaaaatatc gattaaaaaa gtaaacatcc aaaaaagggt gtttactttt
     5041 ttgtttacta atatttttag taaaaaatct cgacaaaaca ttaagtaaac agttatggtg
     5101 ttgttaacaa aaagaaaacg cattcaaaac aggaggaatt aagatgaaac atttcaaaaa
     5161 aatagcttta acagggatag ctgttttaac tgggacatta ctcgcagcat gtggtggggg
     5221 agatagtaat tctaaagacg ggcaagtaac tttaagctat gcgagttgag atactgacca
     5281 agctccagga ctaagaaaaa tgttagatga atttgaaaaa caaaatcctg atatcaaagt
     5341 agaaatggag acaacgcctt gggatcaata ttgggttaaa ttagaagctg caacaacggg
     5401 aggtaatatg ccagatgtag taaccatgca ctctagcgaa tcctataaat atatgtcaca
     5461 gaatgctttg atgggacttg atgatgttat caaagaaaat gatattgaca tgggaaactt
     5521 tactgaagga attgctgatt tctatacgct tgaagacgaa ctatacgcaa tccctaaaga
     5581 tgcttctgta gtggggctat ggtacaacaa agaattattt gataatgcag gtgtcgatta
     5641 tccagatgaa acatggacat gggaaacact cagacaagct gcaattgatt taacagatga
     5701 atcaaaaggc atctatggtt ttgcatcaca tattggaaca gagactggct actggccatt
     5761 tatttatcaa aatgggggag aagtatttgc cgatgacaat acaaaatcag gtttgaatac
     5821 cccagaagca caagaagctt tagcttttta tcgagatatt gcacttgttg atcatgcttc
     5881 accaacaatt acagaattac aagatcctga taagttaagt cgttttcaat caggtagatt
     5941 agcaatgatg attgaaggaa actggtttgc accgggattt ttgaaaaatg actatctagt
     6001 tgaaaatgtt gcttctgccg tgttacctaa gggaaaagtt aatgcaacaa ttacaaatgg
     6061 acttggttgg gctgccagtg cgagtacaaa atacccagaa gaagtgaaaa aactaatggc
     6121 atttttagct agtgaggacg ccaataaaat ccaagcggaa acgggggcat caatcccagc
     6181 actaaaaggc tttgaagacg agtgggctgc ttcttcagat acaattgatt tgagtgcttt
     6241 tggaaaaatg ttggagtatg gagtccctcg tccatacaat aaagatggat tacgagctga
     6301 acaagcagaa gttcaaatta ttaaccgtat tctgtctggt gaagtaactg ttgaagatgg
     6361 aaccgctcaa attgaaaaag aagtcaatgc gatattgagc ggaaattaaa ttagttaatt
     6421 atacaatata caggcgaaag gattctattt cgtctgtata ttaatggatg aataccaaaa
     6481 agggggatat tatgaaaaat cgaatttttg gaaaatatcg taaagatcaa gtgatatggg
     6541 gctatatttt tatttttcca acggcattag gattaatttt attaaatata tggccagcaa
     6601 ttcaaacagc aattttaagt tttcaaaaaa cggtagggtt cggtgatact gaatgggtcg
     6661 gattaagaaa ttaccaaaaa atgtttgcag acggtgaagt ttttcagtct ttattaaaca
     6721 cattaattta tgcagtggtt tcgataccta gtattgttgt attatccttg ttggtggcag
     6781 ttttaatgaa tagaaaagtt aaaggattgt cgatttatcg gacaatttat tttctaccag
     6841 tcgttgctgc accatccgca gtcgcaatga tttggcgctg gatgttcaat aatgattatg
     6901 gactgattaa taacatgttg gctaaaatag gtctcgatgg accagcatgg ttaacggatc
     6961 catcgattgc gatttattct attatcattg taggagtttg gagtgctatt ggttataaca
     7021 tggtgttatt actagccggc ttataagaaa ttcctaaaga ttattatgaa gcagcggaaa
     7081 ttgatggtgc tagtccaatc cagcagtttt tcaacattac gttaccatta gttacaccaa
     7141 cattattttt tgtagttgta acaacagtta tcaatgcttt ccaagtattt gatgttattt
     7201 ttatgatgat tactcctaat agtaccgcca tgtttaaatc acaatcatta gcctatttat
     7261 tctataaaca ttcatttatt ttaaatgaca agggatatgg ttcggcaatc gttatgttcc
     7321 ttttagtgat tatattaatt attactgcaa ttcaaatgaa gttgcagaaa aagtgggtaa
     7381 actattaagg aggctatcaa attatgaaaa ataaattttt ttcaaatgcg ccaatccata
     7441 tcatgcttat tttaggtggt attgcaatgg cattaccttt tatttggatg attttaactt
     7501 cagggaaaac gttggttgaa tctacccaaa ttccacctac aattttccct gagaaatttc
     7561 agctaagtaa ctatcaacaa gtacttgatt tattaccatt catggctttt tatatcaata
     7621 catttttaat ggttgttgga cgagtgattg gctcggtttt attttcagca atggctgcat
     7681 atgcttgtgc gcgtctaagt tttccaggga aaaatatcct tttcagctta gtacttattc
     7741 aaatgatgat tcccggttcc atttttatta ctccacagta tttattagca caaaaattgg
     7801 gtattttaaa tacaattcca gcattaatat taccaggggt agttagtgcg tttggtacgt
     7861 ttttattaag acaatttttc ttaggtttac ctgatgaatt ggaggaagcg gcctatttag
     7921 aaggtgccaa tatttggacg atctttttca aaattatgtt gccgcttgct aaatcaggat
     7981 tggttgcact tggaatattc acagcgttgt ttgcatttaa agatttaatg tggccattga
     8041 ttgtcaatac ttcaattgat aagatgcctt tagctccggg attagcaaac ttacaaggac
     8101 aatatgccac gaacttccca caattaatgg caggttcgtt attagcaatt tggccaatag
     8161 ttgtgatatt catcgttttc caaagaaaat tcattgaagg aattgctaca acaggtagta
     8221 aattataaaa ctattaatta gtgattggag agtaagtaca tgacaaaaat tacatttatt
     8281 ggtgcaggaa gtactatttt tgcaaaaaac gtattaggcg atgcgatgtt aactccaagt
     8341 ctacaagatg cacatattgc attgtacgat attgatgaaa aacgactaaa agaatcaaat
     8401 ttaatgctta aaaatattaa taaaaattcg aatgaaaatc gtgctgatat tgtttcttat
     8461 tatgatcgca aagaagcgtt aagagaggct gattttgtta tcaatgctat tcaagtgggt
     8521 ggttataaac cttcaacagt gattgatttt gatattccta aaaaatatgg gttgcgacaa
     8581 actattggtg acaccatggg gattggtggg atttttagaa ccctacggac actaccagta
     8641 atgttagact ttgctaaaga aatggaagaa gtttgtccaa atgcttggtt attgaactat
     8701 acaaatccga tggcgatgtt aaccatgggt gttttaaaag cgacgagtat taagactgtt
     8761 ggtttatgtc atagcgttca agtatgcgta ccagaattgt ttgagcattt aggaatagcc
     8821 gatcaatatg acttaaatga ctttcaatgg aaaattgcag gcattaatca tatggcattc
     8881 ttgttggaaa ttacaaaaaa tggggaagat ttctatccaa caattcgtca gttagctgca
     8941 gaaaaaccaa atccacatcc agattctgta cgttttgaat tgttgaaacg ttttggttac
     9001 tatattacag aatcaagtga acacaatgca gaataccatc catactttat taaatcaaaa
     9061 tacccagaat tgattgacga attagggatt ccgttggatg aatacttacg tcgttgtgaa
     9121 caacaaatta atgattggga aagcatgcga gaggacattg taaataatgg ggagttaaca
     9181 catacgagaa gccgtgaata tgcttcatat atcatggatt ctattacgac aggaactccg
     9241 tcagtgattg caggaaatgt cttaaataaa ggcttaatca caaacttacc gacagatgtt
     9301 tgtgtggaag taccttgttt agtcgataaa aatggcgtgc aaccaaccta tgtcggtgaa
     9361 ttgccaacac aattagctgc tttaaatcgg acgaatatca atgttcaaga actgacagta
     9421 gaagccgctt taacactaga taaatcaaaa atttatcaag cagcgtattt agatccacat
     9481 ctatcatctg aattgtcttt aacagatatc aaaaatttag tggatgattt aatcgaagca
     9541 catggggact atttacctga atataattaa aaagaagctg aaagagtaag gaattttcaa
     9601 ttccttactc ttttcttagt taaatttagt aaaaatattg acaaaacact tagtaaacag
     9661 ttacattagt gttaacaaaa agaaaacgca ttcaaattgt ataaggagga gcataaaatg
     9721 aagtggtcta aaaaaattat tttagcttgt atagcactag gagcaacgat ctctttgact
     9781 gcttgtggaa aaaatcagga agatgcaagc ggtaaggtag aaatagagtt ttttaatcaa
     9841 aaaaaagaaa tgactcaaac gattcaggag attgctaaag attttgaagc aaaaaaccct
     9901 gatattcatg taaaagttgt ggatgtaccg aatgctggtg aagtaattaa aacacgtatg
     9961 cttgcaggcg atgtaccaga tgtcattaat ctttatcctc aaagtataga attgaaagaa
    10021 tgggcgaaag ctggttattt agaggactta accgaagaac cgtatttaga gaatatcaaa
    10081 aatggatatg cacaacgttt tgctattgag gatcgtgtat atagcatacc attaaccgct
    10141 aatgtctatg gtttttacta taacaaaact gcttttgagg aaatggggat tcaagcacca
    10201 gaaacttggg cagaatttga aaaaatagta gctgagatta aagatcaaaa caaagtaccg
    10261 tttgcgattg caggagcaga aggctggact ttaaatggtt atcaccaact agctatagcg
    10321 actgctgctg gtggtgaaga ggaagcgaac aatgtttggc gtttctcaga agttaatgga
    10381 attaatgctg aaagcaaaga aatgcaaaaa gattttaatc gattagattt gttaagagaa
    10441 ccaaaagctt tgcaaaacaa ctggcaaggt gccggttata acgatacagt agttactttt
    10501 acaaaaggtg atgcgttgat tatgccgaat ggttcatggg caatgccgat gattcattca
    10561 caaaatccag attttgaagt aggaactttt ccttttccag cagatgaagc aggtcaaagt
    10621 ctaacgattg gcgcaggaga cctagcctta tcagtttctg caacttctca acataaagaa
    10681 gaagccaaac gttttgtaga atatatgaca acacctgaag cgatgcagaa atattatgat
    10741 gtagatggtg caccgtgtgc tgttgagggc gttatcgaga atactcagga ttcaccatta
    10801 agtggtttaa ctgaacttgc ttttactgat cgtcatttag tttggttagc caaagattgg
    10861 aatagtgaaa atgattttta taccttaaca acaaattatt tacacaatgg caatcaaaaa
    10921 gccatgatta acgcattaaa tgctttcttt aatccaatga aagctgacgg aaggtaggaa
    10981 aataaaatga aaataaaaga aattttaaat cgttactggg caatcatttt tgtagcaatt
    11041 ccaattattt tgcagatgat ttttttctac tttccaatgg ttcaaggtgc tttttacagt
    11101 ttaactaatt ggactggtct gacttataat tttgattttg tgggtttgaa caattataag
    11161 attctttttt ctgatccgaa gtttatcaaa tcgattggct tcactttaat tgtgacgctt
    11221 tgcatgattg ctggacaaat tggttttggt attttgattg cgcgggcgtt gaacgcaaat
    11281 attaaaggga aaacattctt tagagcatgg ttcttttttc cagcggtctt atcgggcgtg
    11341 gttgtttctt tagtatttaa acaaattttt aactatggtt taccaaaaat tggtagtatg
    11401 ttaggcattg aatttttaca aaccagtctt ttaggaacaa catggggagc gatttttgca
    11461 gtaatttttg ttttactttg gcaaggaatt gccatgccag tcatcatttt cttagcgggt
    11521 tttcaaagta ttccacaaga aattattgaa gctgctagaa tggatggggc gaatgataag
    11581 caaatttttt ggaatattga aatcacctac ttattaccaa gtatttcaat ggtatttatt
    11641 atggcattaa aaggtggctt aacagcattt gatcaaattt ttgctttaac tggtggtgga
    11701 ccaagcgatg ccacaacatc tttggggtta cttgtttata attatgcttt taaaaacaat
    11761 cagtttggtt atgcgaatgc tattgcttta gtcttattta tcttaattgg gattgtttca
    11821 ctggcacaat taaaattatc gaaaaaattt gaaatctagg aggtttccca atgaaaacat
    11881 caaaagaaaa aaatatttct aaatacttgc ttttattatt agggggcttc atgattttag
    11941 ttcccttagt ttcaacgcta ttcggttcat ttaaaacaac acccgatatt atgaataatt
    12001 tctttcgttt tcctaatcca gcgactttgg aaaatttccg aagattgtta gcggatggaa
    12061 ttggtcatta cttttggaat tcggcaatca tcacaatcat tgcagtagtt gctgtgacag
    12121 caattattcc tatggctgct tattcactag caagaaaaat gactcgtggg aaagcatacg
    12181 gattgatgta tttattttta attttaggaa tttttgttcc tttccaagta attatgattc
    12241 cgattaccgt tatgatgagt aaaattggtt tagcaaatat gtggggatta atcttactct
    12301 atctcactta tgccgtacca cagacactat ttttatatgt tggttttatt aaactaaatg
    12361 tgcctatcag tctggatgag gctgctgaaa ttgatggggc tagtcacttt acaacttatt
    12421 tcaaaattgt tttcccaatg ttaaaaccaa tgcatgcgac gactattatt cttaatgctc
    12481 tatggttttg gaatgatttc atgctaccat tattaatttt aaatcgtgat tctaatatgt
    12541 ggacactacc attgtttcaa tataattata cgggacaata ttttaacgac tatggtccaa
    12601 gttttgcttc ctatacagta ggaattgtca cgattacact agtttatctt gctttccaaa
    12661 gaagtattat agctggaatg agcaatggag cagtgaaata accaataaat tattctcatt
    12721 ataaaggagt tcataaactt atgattatat ttgacaatca cacacaaaca tttcatttac
    12781 aaaataaaaa aattagctat ctattaggaa tcgaagaaaa taattattta acgcatcttt
    12841 attttggaaa aaagattacg cactattctg gtgggtatcg ctacccccga acagatcgtt
    12901 ccttctcacc caacaatgca aatgcgcaag atcgtttatt atctttagac acattaccat
    12961 tagaatttcc aggatatgga catggtgatt ttagagaacc ggcgcataat attaaattag
    13021 gaaacggtag tcgtgtaaat gattttcgtt atgatagcta cgaaattcaa aaaggaaagc
    13081 ccagcttagc gggattgcca gccttatata cggaagatga tgatgaagct gaaacgctga
    13141 ttattcaatt gaaagacgcg attagtggat tgactttaca gttgttctac acaatttatc
    13201 aaaatcacag tgtaattatt cgttctacgc aattagtaaa taatagtgat gaaacagtgg
    13261 agattaaccg cttagctagt caatcaattg attttccaaa tcgtccactc gagttaattc
    13321 atttaaatgg tacctgggca agagaacgtc aaatgactag agaaaaaatt catacaggga
    13381 taaaagtatt agatagtaaa cgcggatcaa gcagccatca acaaaatccg tttgttgctt
    13441 tagtagatgc caaaacagat gaatttcaag gagaggcata tggatttaat ttagtttata
    13501 gcggtaatca cgaaacggtg attcaaaaag atccatttga ccaaacacgg gtcattatgg
    13561 gaattaattc atttaatttt ggttggcaac taaaaactgg cgactcattt caaacgccag
    13621 aagtcgtgat ggtttatagt aatgaaggtc tgaatacgtt atcacagacg tatcatgaat
    13681 tgtataatca ccgtttaatt cgtggaaact atcgtttaaa agaacgtccc acgttaatca
    13741 acaactggga agcaacgtat tttgatttta acgaagaaaa aatcatgaat attgtcgatg
    13801 atgcgaagaa cttgggcatt gaaatgtttg ttttagatga tggttggttt ggcggtcgtg
    13861 aaaatgactt cacttcatta ggtgattggg ttgaaacaga aggcaaactt gcttgtggtt
    13921 tggaaaattt agccaagcaa gtgcatgata acgggatgaa atttgggatt tggtttgaac
    13981 cggaaatgat ttctgaagat agtgaactat ttcgagcaca tccagattgg gcattgggta
    14041 ttccaggtcg tgaccgctca attagtcgaa atcaatatgt tcttgatttt tcaagacaag
    14101 atgttcgtga taatatctat caacaaatga cggcgatttt tgatcgtgta ccgattgatt
    14161 atgtgaagtg ggatatgaat cgaaatatga cagaagtcta ttctgtcttg ttggactcaa
    14221 cgatgcaagg tgaagtggct catcgttata tgttagggtt atatgaattt ttagaaaagt
    14281 taacaacgaa ttatccaact attttatttg aaagttgttc gggtggaggc ggtcgttttg
    14341 atgcaggttt cttgtattac atgccacaaa cttggacgag cgacaatacc gatgccgtgg
    14401 ctcgtctgaa aattcaatat ggaaccagct tagtgtatcc aatttcaagt atgggtgcgc
    14461 atgtttcagc agtcccaaat catcaaacag gaagagaaac gagtttaact attcgtggca
    14521 atgctgcgat ggctggtgtt tttggttatg agctagatct aacgcaatta acttctgaag
    14581 aaaaagcaga aattaaagaa caagttgcct tttataaaaa gcatcgccaa cttttgcaat
    14641 atggaacttt ttatcgcttg gaaagtccgt ttgaaggcaa tgatactgca tggatgtttg
    14701 tctcaaaaga ccaaacagaa gccatggtct tttatttccg agtattagca gaagctgcgc
    14761 atccattagt cacattgaaa ttagcaggat tagcagaaga agcaacttat caaatggaag
    14821 atgtaacatt tgctggcgat gaattgatga atctcggttt ttatattgac tcagaactac
    14881 atggtgatta tgcgacacaa cgcttttatt tgaaacagat atagtagctt gaatttacaa
    14941 tataagtgca acaccaaaag agactcattc caaacgaaga aaatatgttt tatttacacg
    15001 catttactaa aaagcagcct gatttgaatt gggaaaatga agaagtacgt gaagaaatct
    15061 atgcaatgat taactattgg ttagataaag gactaggcgg atttagaatc gatgcgatat
    15121 taaacatcaa aaaaaaacta gagtatggca tctttgaatc tgatggagaa gatgggttag
    15181 ccttcattgg gccctggatt cttaatcagt cgggcattga agtgtggtta aaagaaatga
    15241 gagagcggac atttaaacca cataatagta tgacggtagc agaagccgat gttcctaatg
    15301 aacgtttaga tgaatatatt ggtgaagatg gctattattc gatggttttt gattttagtt
    15361 atacggatat tgatgtacct gaaactggtg aatggtttaa agatagccaa tggacatgga
    15421 cggatatgcg taccaatatt tttacgaatc aattagttac gcaagataaa ggttggggcg
    15481 ccttatattt agaaaaccac gatcaaccac gttcaattaa taaatatatt ccagaagaat
    15541 ggatcaatga ttatagtaaa aaaatgttgg cgacgctttt tatgttacta agaggtaccc
    15601 cttttattta tcaaggacaa gaactaggaa tgaccaacat agaaatggca tcattagaag
    15661 attttgatga tgtagctacg cattctcagt ataaacgggc attagaatat ggattggcac
    15721 cagagcaagc attgaaagct gtcaataaac gaagtcggga taattctcgc acacctatgc
    15781 aatggaatac gcaaaaaaat gccagattct caacttctga taatgtatgg ttaaaagtaa
    15841 atcctaatta tccagaactc aatgcagaag ctcagttaac caatcaacag tcggtattca
    15901 attattatca tcaattaatt aaattaagaa aaaatagtgt atacaaagac gtgctaatct
    15961 acggacaatt tctaccagtg aaagataaaa atgaacatat tttgctttat gaacgccaat
    16021 tagaagataa aatcgtatta gttatcttaa acgttacagc aacagaacaa gaatattatg
    16081 tagatgaaca gtaccaacaa gtattaatca ataattatga agatgtttta ttagatcaaa
    16141 gtcgaaaatt gcgtttacga ccgtttgaaa gtattgtatt agctaattat ggaggacaat
    16201 tatgaaaatt aaaaacgaag caatgctcat cacctatgca gatagtttag gaaaaaacat
    16261 gggcgaatta aaacaagtct tagacaaaca tttacaaggt gttattggtg gtgtacatct
    16321 gttgcctttt tttccatcga caggtgatcg aggctttgcg ccgagtgatt atacgacagt
    16381 tgatccagct cttggaacat gggaagatgt ggaagcatta ggtgaagatt attatttgat
    16441 gtttgatttt atgattaatc acatttctcg tgaatcaaaa tttttccaag atttcaaaaa
    16501 aaaccatgag cagtcaccat acaaagaaat gtttatccgt attcatgaat ttttcccaga
    16561 aaatcgacca acacaagaag atattgattt aatctacaag cgtaaagaca aagcgccgtt
    16621 tcaaacggta gaatttgcgg atggtacgac agaagaggtt tggaatacct ttggggaaga
    16681 acaaattgat ttagatgtga ccaaagaagt tgtgaaagaa tttattcgag aaaccatcaa
    16741 agatatggcg aaccatggct gttctttaat tcgattagat gcgtttgctt atgcgattaa
    16801 aaaattagat accaatgatt tcttcgtgga accagatatt tgggaacttt tagacgaagt
    16861 gcgtcaagaa gcagcgaaat atgacgtcga attattacca gaaatccatg agcattattc
    16921 cattcaaatg aaaattgcca atcatgatta ttatatctat gacttcgcct tgccaatggt
    16981 cactctgtat tcactttata gtggaaaatc agaacgttta gccaattggt tgaaaatgag
    17041 tccgatgaag cagtttacaa cattggatac gcatgatgga attggtgttg tggacgctcg
    17101 tgatttatta acagatgaag aattggatta cacctctgag gaattgtata aagtcggggc
    17161 gaatgtgaag aaggtctatt caagtgccaa ttacaataat ttagatattt atcaaattaa
    17221 cagcacctat tatagtgcat taggcgataa tgaccgtagc tatctgttag cacgtgcgat
    17281 tcaagtattt gcgccaggga ttccacaaat ttattacgtt gggttattag caggaaaaaa
    17341 tgatattgaa ctgttggaag caacgaaaga agggcgtaat attaatcgtc attactatga
    17401 tttagccgaa attgaagaag aagtgcaacg gccagtgatt caacaattat ttgcactttt
    17461 aaaatttaga aatgaatctg cggcgtttga tttggatgga acgattgaag tagcaacgcc
    17521 atcagaatca gaaatagtga ttacacgtaa aaataaaaac ggtagtgata gtgcaacatt
    17581 aacggctaac ttggatacga aagaatttat cattgagtct aaagatataa aaattttcac
    17641 tagtgttgca ttaaaatttt aacctattcc atagaacaaa aaatccttta tactagcact
    17701 tggatgactt atccaagacc aataaaaagg actcatacat ggataagtat aaaacaaata
    17761 cagcatttaa taaatggttt tctgccataa aattaggaaa actttctgtc aactcccaaa
    17821 aatgtatttt tgattacaat tcgtatcgta aaaaactgtc ttttgaagct gtgctcaagc
    17881 tctttctcta cgcagttaat gaagaaaaag aaagtttgcg tgatttgagt acagctctta
    17941 tcgaccgatc gctccaacta gaaaccgatg tgtcatcgat tagtcatacc caattgtctc
    18001 gagcatttaa tgcacttgaa cctaaagtgt tagaagaaat ttttcaacaa ctgttagaaa
    18061 aagttcaaca tcaaatcaaa ccaaccaagc gaaatcatct gtatctgatt gattccagta
    18121 cgttttcgct tagtttaaaa cgtcataagt gggcgacttt ccgtcaaaca aaatcaggtg
    18181 tcaagttaca cttaaactat tgttatatgg acgatactac gatgtatcca actgatttta
    18241 ccttaacaaa tgcccatgaa catgatctca atcaactacc agtattggtg aatcaaccag
    18301 aggcaactta cgtgtttgat cgtgggtatt aggactttga aaagatggat cagatgcatt
    18361 gggatggcta tttcttcgtc actcggatta aaaaaaacac caaagttcac gtgatagata
    18421 ctctggaaac atcgccagaa acagaaattc tacgagacga gctggttcgt ttaggatcta
    18481 aaacctatct aaccgccaat ttccgactag tcactgttca agataagaac ggacgtgtgt
    18541 ttcaatttat caccaatcga atggacgtct cttcaaaaga aatctctgat atgtatcacg
    18601 ctcgttggca aatcgaattg tttttcaagc acatcaaaca acacatgacc attaaaacct
    18661 tcttttctca aagtgaaaaa ggcgtgcaga atcaattgat tttaacgatg atttccgctc
    18721 tcctcacgtt tctcatcaaa ttggagacga aaacggaaaa atcagtgttc caaatcaaac
    18781 gattctttcg ttatttactg tttcagccct ttgaatgttg tctagaaaaa ttaattccca
    18841 catgagaaaa aagaaatttg gctcgttact tttggttttc agttgaataa cagataaaaa
    18901 attttctgga aaaagtcatc ctctgcgtca gcacagtttt ttttgactta ttttcagagg
    18961 aaatagtttt cagaatattc taaaatgttt tatgcaacac tagtgatatt ttttagaatt
    19021 agatttctaa tgtgca
//
LOCUS       DARDHA010000040        19015 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000040 DARDHA010000000
VERSION     DARDHA010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.040"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(112..471)
                     /gene="rplT"
                     /locus_tag="SDA10_001141"
     CDS             complement(112..471)
                     /gene="rplT"
                     /locus_tag="SDA10_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEG4240065.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(524..724)
                     /gene="rpmI"
                     /locus_tag="SDA10_001142"
     CDS             complement(524..724)
                     /gene="rpmI"
                     /locus_tag="SDA10_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEG4240066.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(796..1320)
                     /gene="infC"
                     /locus_tag="SDA10_001143"
     CDS             complement(796..1320)
                     /gene="infC"
                     /locus_tag="SDA10_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEG4240067.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1364..1489)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1640..2272
                     /locus_tag="SDA10_001144"
     CDS             1640..2272
                     /locus_tag="SDA10_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEG4240068.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2274..3179)
                     /locus_tag="SDA10_001145"
     CDS             complement(2274..3179)
                     /locus_tag="SDA10_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG4240069.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3264..3923)
                     /locus_tag="SDA10_001146"
     CDS             complement(3264..3923)
                     /locus_tag="SDA10_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG4240070.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4239..5183
                     /gene="mvk"
                     /locus_tag="SDA10_001147"
     CDS             4239..5183
                     /gene="mvk"
                     /locus_tag="SDA10_001147"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEG4240071.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5176..6153
                     /gene="mvaD"
                     /locus_tag="SDA10_001148"
     CDS             5176..6153
                     /gene="mvaD"
                     /locus_tag="SDA10_001148"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEG4240072.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6166..7251
                     /locus_tag="SDA10_001149"
     CDS             6166..7251
                     /locus_tag="SDA10_001149"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEG4240073.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7248..8312
                     /locus_tag="SDA10_001150"
     CDS             7248..8312
                     /locus_tag="SDA10_001150"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEG4240074.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8561..9364)
                     /locus_tag="SDA10_001151"
     CDS             complement(8561..9364)
                     /locus_tag="SDA10_001151"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEG4240075.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9384..10226)
                     /gene="kduI"
                     /locus_tag="SDA10_001152"
     CDS             complement(9384..10226)
                     /gene="kduI"
                     /locus_tag="SDA10_001152"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEG4240076.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10242..11249)
                     /locus_tag="SDA10_001153"
     CDS             complement(10242..11249)
                     /locus_tag="SDA10_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEG4240077.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11256..11900)
                     /locus_tag="SDA10_001154"
     CDS             complement(11256..11900)
                     /locus_tag="SDA10_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEG4240078.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12000..12758)
                     /locus_tag="SDA10_001155"
     CDS             complement(12000..12758)
                     /locus_tag="SDA10_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEG4240079.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12885..14036)
                     /gene="fucO"
                     /locus_tag="SDA10_001156"
     CDS             complement(12885..14036)
                     /gene="fucO"
                     /locus_tag="SDA10_001156"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HEG4240080.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14118..14429)
                     /gene="rhaM"
                     /locus_tag="SDA10_001157"
     CDS             complement(14118..14429)
                     /gene="rhaM"
                     /locus_tag="SDA10_001157"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEG4240081.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14431..15276)
                     /gene="rhaD"
                     /locus_tag="SDA10_001158"
     CDS             complement(14431..15276)
                     /gene="rhaD"
                     /locus_tag="SDA10_001158"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HEG4240082.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15290..16546)
                     /gene="rhaA"
                     /locus_tag="SDA10_001159"
     CDS             complement(15290..16546)
                     /gene="rhaA"
                     /locus_tag="SDA10_001159"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEG4240083.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16543..18003)
                     /gene="rhaB"
                     /locus_tag="SDA10_001160"
     CDS             complement(16543..18003)
                     /gene="rhaB"
                     /locus_tag="SDA10_001160"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEG4240084.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18156..>19015
                     /locus_tag="SDA10_001161"
     CDS             18156..>19015
                     /locus_tag="SDA10_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEG4240085.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFR"
BASE COUNT         5336 a         4157 c         3497 g         6025 t
ORIGIN      
        1 ttttagactt atacgttatt gtcctgtttc atttgatgga ggattgtaaa taaaaaaaga
       61 ggaaagaaca taacttttcg ttatcttctt tcctctttat aattgtttag cttatttagc
      121 caaagcttct tttgcttggt cagccaatgc agtaaatgct gctgcatcgt taacagccaa
      181 gtcagctaac attttacggt tgatgtcgat ttcagcaact ttcaaaccgt gcattaattt
      241 tgagtagctc aagccattca tacgagctgc tgcgttgata cgagcaatcc acaatttacg
      301 gaagtcgcgt ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat tcattacttg
      361 ttcttttgct gttttaaata aagtgtgttt tgaaccgtag taaccttttg ctaattttag
      421 cactctttta cgacgtttac gagttactgt tccaccttta acacgtgcca tgtataattc
      481 ctcctaataa aaataaatct tcagattctg ttatttgtac ttcttatttc atttttgcta
      541 gttgttggcg aatacgtttg aagtcgccac tagaaaccat gcttgcttta cgcaattgac
      601 ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg aagcgtttca
      661 atccgccttt accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt ttttgttttg
      721 gcatgactat atttcctcct caaaatcttt cgttatccga ctatcggctg tccaacagcc
      781 agttagtcat tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga acatgctgcg
      841 tccgtccatt ttcgcttttt gttccactgt tgcaatatcc gcagtttctt cagctaagcg
      901 attcaagact ttttgaccaa tttctttatg ggtaatggca cggcctttga aacggataga
      961 agctttcact ttatcgccct tttccaagaa cttacgtgca ttacgaagtt ttgtattgaa
     1021 gtcattcaag tcgatcgtag gacttaagcg aacttctttt acattgatca ctttttgttt
     1081 tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt agtccatgat
     1141 tcgcgcaact ggtggtttcg caccaggggc aactaacaca agatccaagt ttgcttgttc
     1201 tgcaatattc aatgcttctg cttttgtctt aactcctaat tgctcaccat ctgaaccgat
     1261 caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc atatcctttg ctatggtcat
     1321 tcacctccaa atattatgag agaaagaaaa gcttaccgaa taaacggcaa taaaaaaacg
     1381 agttctctta cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa ctatctagcc
     1441 cagtgacatt atcaactaag gcgagaagcg ggtgcttctg cttttatttc tcaaccatca
     1501 tagtatagca aaaactaatc tttttgtcaa tgtttttcat acagtttgtc ttccttttct
     1561 gaaaatgatt agattagcag actttctagc agtgattccc gcttctttgc tatactaaag
     1621 aaaaacgagg tgaacccaca tgaaaaaaat cgaacctatt ccaccttctc ttcctcttct
     1681 tacaaaggga gagttttatt tagaagatga ggagatcttc tctggtcttc tgatcaacgg
     1741 gggtgaccaa agctatctcg atgccaaaaa tatggtatta agagagagtc atatccaaaa
     1801 acttacgatg caaaaaacag ttttacgtca ttttgaatgc agtaatgtca tttttgaaaa
     1861 atgtgatttt tccaatctgg agtggattgg tggcagtttc caccaagtcg tgtttcatca
     1921 atgtaaatta acaggtacga attttgccga aagttatctt cgagactgta ctttcactga
     1981 ttgtattgct tcgatggctt cctttagtag tacaaacttg aaagctgtct ccttcgatca
     2041 ttgtcaatta gaagacagcg aattttatga ggtcacttgg aaaaaccttt ttttgtctga
     2101 caatcagttg accggttcga actggtttcg aacttctctg aagggattag actttacgac
     2161 taatttcttc acagcaatcg ctttatctca agattctttg gcgggactaa tcgtcaacca
     2221 agagcaagca cttgtgatcg cccaagccct tggtttgcag atcaaagact agatcaacga
     2281 atccgccgcc acagtgcaat aagcatccaa atagataaga ccaaactgat gatcaaggcg
     2341 attccccatc cgaaaacgga atgggtaaag ggtaactgga gattcattcc gaagaatcct
     2401 gtcacaatag ttggaacggt caagatcagc gaccagaccg tcaaaaattt catcgtgtca
     2461 ttcaagttgt tattcagcaa attattataa gtgccagaga gctggtctaa aatctgcgaa
     2521 gctaagttcg tcatttcaac tgcttgtcgt gcttcgatca gcgcatcgtc caactcttct
     2581 ttttcctttt ctgacaactt gcgataaata gccaatgcct tgatttgttc taaaagtact
     2641 acattttgtt ttgttcctgt aactaagtaa accaaaccga tctctaaatc tgaaagttgc
     2701 agcagattct tgttcgttgt tttttctctt aatttcagat taagccgctg acgttcacta
     2761 ttcacttcct cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt acgaaacaat
     2821 aaactgtata cactcatgtc tggcattcgt tccaaaagcg atcgaattag tcgaatcacg
     2881 tactcggtat tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat aaaagcgata
     2941 ggactagttt cataatgatt atcgactttt tgctggtgcg gaacatttga tacaagcagt
     3001 aacgcttcat caaaagaatc gtactctacg cgcgcccgtt catttttatc tagagaataa
     3061 gtcagcatct cgtcactaat gccatattct tcttttaagt ttttaacagc tagcacatcg
     3121 ttttgctgta cattgatcca aaaattacgg tcttgattaa aatagtgttt ttcccgcata
     3181 ctctatcctc ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa taaaggttta
     3241 ccctttattt gagcaactgt aaatcaaatg gctactttgg tttggcgaat ctctgctaat
     3301 ttttcttcga tcattttcaa gacttcttct tttgcttgag gatcttccac aaaattatag
     3361 cggtctccat cgatttggat ttttggactt tctttgtatt cttcatacca ttgatcgtaa
     3421 cgtctgttca attctttgta ataatcgtat agacttggat caaaggacaa ttgttcatac
     3481 gaacgaccac gtttttcgat acgctccaac atcgtatcaa aagatacacg aatatgaact
     3541 aataaatccg gatgtttttt atgggcagca tagggaagtt cttccatcat gttttccagc
     3601 agttcatcat acacacgaac ttctgtttct gttgctcttc ctagatctgc atttagatgg
     3661 aaaagcagtg aatcttcata aatcgaacgg tccaatacat tattgtcatc tttcattgct
     3721 tgtttgatgc ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa agcatactgt
     3781 tcagggtttg cataaaataa gggcaagact tcattatcat caatcgattc ataaaatgcc
     3841 tgactatcaa agtgttcagc catcatttct gttaaacttg attttccggc accaattgta
     3901 cctgctaaaa cgatcacgct catttacctc cagctcactt catcactaaa tattgtgttt
     3961 aactccaacc tttttaaata tacccccaat atctagtagt gtcaattgta tttttttcta
     4021 gtcaaagaag aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc tttagaaaac
     4081 gtattcttag ttttacttct gagatggttt tagtaaaata aaggaggaat gttttttgag
     4141 acccttagtg gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg ctttattttt
     4201 tgcttaaaaa tcacttttac gtgagaagga gttagattat ggcaaactat ggccaaggag
     4261 aatcaagcgg aaagatcata ttgatgggcg agcacgcggt tgtttatgga gaaccagcga
     4321 ttgcctttcc tttctatgca acaaaagtca ccgcattcct tgaagagctg gatgcaatgg
     4381 acgatcaact ggtttcttcc tactattcag gaaatttagc cgaagctcct catgcattaa
     4441 aaaatatcaa aaaattattc attcacttaa aaaaacagca tgacatccaa aaaaacttgc
     4501 aactgaccat tgaaagcacg attcctgctg aacgtggaat gggatcaagc gctgcagtcg
     4561 ccacagcagt cactcgtgct ttttatgatt acttagcatt tcctttgtct cgtgaaatac
     4621 tattagaaaa tgtccagctt tcggaaaaaa tcgcccacgg taatcctagt ggaatcgatg
     4681 cagccgctac tagcagcttg cagccgattt attttacaaa agggcatcct ttcgactact
     4741 tttctttgaa catcgatgct tttttgattg tcgctgatac aggaatcaaa ggacaaacaa
     4801 gagaagccgt caaagatgtc gctcacctct ttgaaaccca gcctcatgaa actggacaaa
     4861 tgatccaaaa attaggatac ttgacgaagc aagcaaaaca agcaatcatt gaaaattcac
     4921 cagaaacgtt ggcacagaca atggatgaat cacaatcact tctggaaaag ctgacaatca
     4981 gcaatgattt tcttaatcta ttgatccaaa cagcaaaaga taccggggca ttgggtgcca
     5041 aattaactgg cggtggacgc ggtggctgta tgattgcatt agcacaaaca aaaacaaaag
     5101 cccaagaaat cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg atacaaggat
     5161 taggagtaca tacctatgtt taaaggcaaa gcacgcgcat ataccaacat tgctctaatc
     5221 aaatattggg gtaagaaaaa tgaagaactc atccttccaa tgaacaacag cctttcgtta
     5281 acattggatg cattttatac agagacagaa gtcatctttt ctgatagcta tatggtagat
     5341 gaattttatc tagatggcac cttgcaagac gaaaaagcaa caaaaaaagt cagtcagttt
     5401 cttgaccttt ttcgtaaaga agccggcctc tcactaaaag cttcagtaat cagtcagaat
     5461 ttcgttccta ccgcagctgg attagcctcc tctgccagcg ggctagctgc tttagcaggc
     5521 gcttgcaata ctgctcttaa gcttggatta gatgatctct ctctttcaag atttgctcga
     5581 cgcgggtctg gttcagcttg ccgaagtatt ttcggtggtt tcgtcgaatg ggaaaaaggc
     5641 catgacgact taagttctta cgctaagcca gtcccttccg attctttcga agacgattta
     5701 gcaatggttt tcgttttgat caacgaccag aaaaaagaag tgtccagcag aaatgggatg
     5761 cgtcggacag tcgaaacatc cagtttttat caaggctggt tagattccgt tgaaggggat
     5821 ctatatcaat tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg agaaacgatg
     5881 gaaagaaacg gactaaaaat gcatggaacg actttagctg cccagcctcc attcacttac
     5941 tggtctcctg acagcttaaa agcgatggat gcggtgcgac aattgagaaa gcaaggcatt
     6001 ccatgctact ttacgatgga tgctggccct aatgtcaaag ttttagtgga aaacagccat
     6061 ttatctgagg tacaagaaac atttaccaaa ttatttagca aagaacaagt aattactgca
     6121 catgctggtc caggaattgc gattattgaa taaagggaga tttttatgat tgaagtatct
     6181 gcaccaggca aactctatat tgccggagaa tatgcagttg ttgaaacagg ccatccagca
     6241 gttatcgctg cagtcgatca attcgtgaca gtcactgtag aatctgcacg aaaagtcgga
     6301 agtatccaat ctgcacaata tagtgggatg cctgtacgtt ggacaagacg caacggagaa
     6361 ttagttttag atattcggga aaatcctttt cattatatcc ttgctgctat tcgcttgact
     6421 gaaaagtatg cacaagaaaa aaacattctt ttatcctttt atgatctgaa agtaacaagt
     6481 gagttagaca gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc cgtgaccgtt
     6541 gcaacggtca aagccttgaa tgttttttac gcgttgaatt tatctcagtt ggagattttc
     6601 aagattgcag cactagccaa tttagcagtt caagataatg gttcctgcgg cgacatcgct
     6661 gctagctgtt atggtggctg gatcgctttc tcaaccttcg accatccttg gctccaagaa
     6721 caagaaactc agcattctat cagtgagtta cttgccctgg attggccagg tctatccatt
     6781 gagccattga ttgctcctga agatttacgt ttattgattg gttggacggg tagccctgcc
     6841 tctacttctg atttggtcga tcaagttcac cgttcgagag aagataaaat ggtggcttat
     6901 cagcttttct taaaaaatag tacagaatgt gtcaacgaaa tgatcaaagg gtttaaagaa
     6961 aataatgtaa cgttgatcca acagatgatt cgaaaaaacc gacaattact gcatgattta
     7021 tctgcaatca ctggggtcgt catcgaaacg cctgctttga acaaattgtg taatttagct
     7081 gaacagtatg aaggagccgc aaaatcttct ggtgcaggtg ggggcgattg cggaatcgta
     7141 attgttgacc agaaatctgg cattcttcct ttaatgagtg catgggaaaa agcagaaatc
     7201 actccactgc cgttacatgt ctatagcgat caaagaaagg aaaaccgatg aatcgaaaag
     7261 acgaacatgt ttcactagct aaagcctttc atgataaaca aaaaaacgaa tttgacttcg
     7321 tacggatcat ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat ctaagtacac
     7381 aggcagttgg gtttacacta agcagcccat tttatatcaa tgcgatgact ggcggaagcg
     7441 aaaaaacaaa aaaaatcaac caagatcttg caatcgtcgc aagagaagca gatttgatga
     7501 ttgccactgg ttcagtcagc gctgccttga aagtcccttc tttagccgat acttatacga
     7561 ttatgcgaca agaatatcct cacgggaaaa tcattgccaa catcggtgca gggacttctg
     7621 tcgaaagagc caaagaagct atccgattgt ttcacgcaga tgccttacag attcatttga
     7681 atgcacctca agagttggtt atgcctgagg gagaccgtga ttttaccaat tggaaagtac
     7741 ttattcaaga aactcaaaca gccattgacg tccccctcat cgtaaaagaa gtcggttttg
     7801 gcatgacaag agaaacgctc aatgatctag ctgctttagg agttcataca gttgatatca
     7861 gcggtcgaag cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc aagcgggaac
     7921 tgagttattt agctgactgg gggcaatcga cagtctcttc attgcttgaa gcaaatgaag
     7981 cagacacttc tatggaaatc ctagcctccg gaggtatccg caatgcctat gacatattta
     8041 aagccctttg tctcggcgca aatgccgtgg gtacttcagg aaccgtactg acgcatctga
     8101 tgaatcacgg cgtagaagaa actattatac tgatgaagca atggcaagaa gagcttcgtc
     8161 tgctttatac tatggtcggc gcaacgaata ccgcggccct acaccaacaa tctctgatct
     8221 tttccggacc agtgaaggat tggtgcgaag caagagggat cgacttagtg aagtatggaa
     8281 gacgcacaga aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca
     8341 cgtacaaacg gcggaagtaa aagcaactcc ttcggaaata agctgaaatt cacaaaaatt
     8401 tgaaaagcaa ttttcgtgaa ttccttctta tttcttggag ttaaatgctt ctgtcccagc
     8461 ctcttttttc gaataaatga tgtttaatca gctgtccttc ggtattcgtt caatccgctg
     8521 atttcacaac ctctccacta aacagtgtac taaagctctt ttaaggttgt tttccgatat
     8581 aggcaagtat gcctccgtcc acatacaaga catggccgtt aacgaaatca gatgcgctag
     8641 aagccaagaa gacagatggt ccagccaaat cgattggctc gccccagcgt gctgctggcg
     8701 tacgtccaac gataaattga tcgaatggat gacgttcacc gttttcttgt gtttcgcgta
     8761 atggagcagt ttgcggtgta gcaatataac ctggtccaat tccattacat tggatattgt
     8821 attgaccgta ttcagaagcg atgtttttcg tcagcatttt taatccgcct tttgccgcag
     8881 cataagcact gaccgtttcg cgtcctaatt cactcatcat ggagcagata ttgatgattt
     8941 taccgccgcc tttttcgatc atatccggaa taactgcttt tgaaacgatg aatggggcat
     9001 ttaaatccac gtcgattact tggcggaagt ctgccgcatc catttcgatc atcggtgtcc
     9061 gtttgatgat ccctgcattg ttgactagaa tatcaactga acctacatct tctttgatct
     9121 gttgaaccat cgcttgaacc gcttcttcat cggtcacatc gcagacataa ccttttgcat
     9181 cgatgcctgc ttctttataa tgcgctatac cttcatcaac tgattcttga ttcaagttat
     9241 taaatacgat ttttgctcct gctgtagcta aagagcgtgc aatctcaaat ccaataccat
     9301 agacagcacc ggtgactaat gctacttttc catctaaacg aaacatatcc atactaaatt
     9361 ccatcatttt ctctccctta tgcttatttt aattgatcca tttgcaccat atccatgtct
     9421 gtataggtga tattttctcc gcacattgcc cagatgaatg agtaattgct tgtacctaca
     9481 cctgaatgga tcgaccagct tggcgaaata atggcttgtt cattgtctac aactaaatgt
     9541 tttgtttcat ctggtttgcc catcatgtgg aacacacgtg tatcttcatt ggcataatca
     9601 aagtagacat aagcttccat acgacgttca tgtgtatgac aaggcatcgt attccaagcg
     9661 cttcctggtt ctaaaatcgt ataacccatt tgtaattgac agctttcgca tacatttgga
     9721 tggatgtatt ggtagatttt acgttcattc agtgtcaggc cttcaccagt ctccattggt
     9781 tttacttggt cgatactgat ttttacattt ggatatttat gatgagctgg aacagaacta
     9841 atatagaatt tagctgggtt ttcagcatct tgagaagaaa atacaacatg tcgtgtttct
     9901 tttccgatat agtatccgtc ttgttttttc atttcttctt tttgcccatc gatctcgatg
     9961 aatcctggtc caccgatatt gatcacacct agttcacgac gctctaagaa gtaatcaaca
    10021 cctagttctt tgtctaatgt gatttctaat tctttttcag taggtgtcac tccaccaaag
    10081 atcattcgat cgttgtgcgt ataagtcaaa ctgattttcc ctggttcaaa taccttttct
    10141 actaaaaatt cttttctcaa ttgttccgtt gaataatgac ggatatcttc tggactgtgt
    10201 gtataacgtg tttccatttc ttgcattttc ttctactcct tctatcgaat gatttttcca
    10261 gagccgcatg ctaaaaattg tgcgacttct tcttgactaa attggttgca gtccccatgg
    10321 atcgtatgct tcaaggcaga agctgccgta gcaaaatcaa tgatttcttg cggagacata
    10381 ttggataaaa tcccatggag cacgccacct gcaaaagcat ctccgccacc tactcgatcc
    10441 acgattgggg tgattctatg gacttgggat tccacatatt catggttcat ccacaatgtc
    10501 cccttcaatt cattttcact tgctgaatga acggtacgct tggtcgaata aaacacctga
    10561 atattcggga acttttcctg cattttttga taataataaa ttagaggttg ttcttctgct
    10621 tctggcgctt tctcgatacc taacaaataa agagcatcca tttttccagc tgagcagata
    10681 tctactaaag gtaaaagctg atggattact tcgctggctt cttgctgtgt ccataatttt
    10741 ccacgatagt tgatatcgaa gctgatcagg cagccagctt tttttgcagc ttctatcacc
    10801 tgtttcgtca ttttttgcca ttttttagac aaagctgggg tgattcctga aacatgaaat
    10861 atatctattc cttggaataa ctcctctagc gaccattcca aatgatccat ttcagcaaaa
    10921 cttgatccgg cacgatcata gatcactgag gcggcgcgtt cgccactacc agtttccata
    10981 taataagtac ctagtcttga tccaccagat aatacctgtt tcgtatctac tccataacat
    11041 tgcaagtgct ttttgactgc ttttcccaat gcgttatcag gtacttttgt agcaaaaatc
    11101 gcttcatgtc cgtaattagc taaagaaatt gctacgtttg cttcccctcc gccataatgg
    11161 gcagcgaatc gttcacttga atcgatacgg gttccctgct ctgtggacaa tcgcaacatg
    11221 atttctccga gtgtcactac ttttcccata agcagctacc tcttgatttc tttcattttt
    11281 gctgcatact gttgcgcgac ttccgttact ttgtcaaaat ctcctgtagc tgcgggtgct
    11341 aaaaggttcc cacctactcc gactgttacg acaccagcat cgaaccactc tttcatattg
    11401 tccagactca caccgccagt tggcatgata ttgacttgag gcatcggcgc cttgaaggca
    11461 gaaatgatac ttggtccata agcgcttcca ggaaatagtt tgacgatatc taccccgctt
    11521 ttcagcgctg ttttgatctc ggtaatagtc atacatcctg gtaagtaagg aatttgatac
    11581 agattgcaca tctctgccgt ttcttgatca aaggaagggc taacaatata ctctgcgcca
    11641 gctaagatag ccaagcgagc tgtgacagcg tctaatactg tgcctgcgcc aatcactatt
    11701 tctggttggt ctttgtagaa tgacaaaagt tctttgatta cttgatctgc ttgagggact
    11761 gtaaaagtca actcaatacc agttagacca cctttgatga ttgcgtggca agcattcaaa
    11821 gcttcttctt tgctttttcc gcgtaccacc gcaatcactc cggcgttttt caatcgagat
    11881 aaaatatcaa tttttttcat cttctccacc tatttcctat ttagaaattt tattttataa
    11941 cttggaaatc tatacccata ttatacggca gaaaagaaaa attgcaaacg cttttttcat
    12001 tatttttgaa ttctttgctc gatttcgctt tttgtttgct tgagggcact gattatttgt
    12061 tcttttgtct catctgttaa acgatatttc ggcatactta cactaaatgc accaatgatt
    12121 tgttcgtttt tcacgattga ggctcctata caaaaaatat cacgttcgat ttcttcatcg
    12181 tcaaatgcga tgtgggtttc ttttaccgtt ttgatttcct ttttcaaccg taatgcattg
    12241 gtgatcgtat attctgtgta agggatcaac ggatgctttt caagataatc agtgtactgc
    12301 tcttcactga attctgctaa tacggctttg cccatcgcag aattgtataa tggtcgggtg
    12361 attccgattt ttgaagacat gcggatcgtt tgatgttttg gatctagttt gttcacgtac
    12421 aagatctcat tgttgtccaa tacccctaag tggatagtct catcgatctt ttgctgcagc
    12481 tgttctaaaa acggtaaagt caactctacc aaatcgattt gttcaatgtt tttattcgcg
    12541 tagcgaataa gctttgcacc taaacgatag tttttctctg aatttttatt tacgtagccg
    12601 atcatcaaca aagtatctag tattttcaag actgtagaag aggtcattcc cacacccttt
    12661 gcgatcgtct gcaaagagac atcttggttt tccgctaaat agtctagtat tttagaggct
    12721 ttgatcaata cagttccata aggcttttta ttttccatat aatattctcc tatttcttta
    12781 taagaaaaat tatagcggaa ataccatcaa tttaaaagag atatccagat ataaactacc
    12841 atttctcttt acaggcaatt taatgttgaa gatccgcttc gtttttacat caaactttga
    12901 tataaagcaa tgatttcttc tactgtcgtt tctcttggat ttcctggtgt gcatacatct
    12961 cgcaaagcat cttctgcaat tgctggaata tctgcttctt ttacacctaa ttcagaaatg
    13021 gtttctggga ttccaaccgc tttgctcaac cgatcgatct caccacaagc cgcttctctt
    13081 acatcttcta aagacatttc agctgctcct ttaatcccta gaacaagagc gatctcacgg
    13141 tacttttctc cagtatattc tttgttatat ttcatgacag ttggcaaaag tgctgcacac
    13201 gctacgccat gaggaatgtt gtaccatgca cttaacgggt gagccatccc gtgtaccagt
    13261 cctaaaccga catttgagaa gcccattccc gcaatatact gtcctaatgc cattttttct
    13321 cgcccatttt gatcgccatc tacagaagct tccaaagaat ggccgatgat ttcgattgct
    13381 tttaaatgca gcatatcagt catttcccaa gcgccttttg tgataaaccc ttcaatagca
    13441 tgcgtcatag catccatacc tgttgatgca gccaattttt tcggcattcc catcatcata
    13501 tcgctgtcga tgaacgcgac gattgggata tcatgaggat caacacaaac aaattttcgg
    13561 tgatttactt catctgtgat gacgtagttg atcgttactt ctgctgctgt tccagaagtc
    13621 gttggtatag ctaatatagg taaacatgga tttttcgtat cagcaacacc ttctaaacta
    13681 atgacgtctg aaaactctgg attcgtcaaa ataatcccaa tggcttttgc cgtatcgatt
    13741 ggcgaacctc caccgattgc gatcaaacag tctgcctctg cctctttaac aaaagccact
    13801 ccatctttta cattttggat cgttggatta ggtacgatcc cgtcgtataa agcataagcg
    13861 attgctgctt tatctagaag ttctgttact tttgttgcta taccgtgctc gatcaatcct
    13921 ttgtcagtaa tgacagctgc tttgccgaat cctcgctgct gaaattcagg gacgatcgac
    13981 tcaatcgctc cttttccaaa atacgatgtt tcatttaaaa tcattcgatt actcattttc
    14041 tattcctcct aatttgtatt atttttcaca ataaaaacga aagaacccca agcaaccggt
    14101 tgcccagttg cttgttgcta cagttcaaag actggcatca attcccaact gactggagag
    14161 gcatctggat ttgtctccat gatatcttcc atgtacttcc accatttttg attgatagat
    14221 gtatttgcca ttttttccca taacttttca tcttcgatca ctaagtaacc aaataacaaa
    14281 cttgtactcg gatcaagata gatcgaatag tcgatgaccc cgtgttcacg aagtgctgta
    14341 cgcatttctg gccaaagttc atcatgtctt tttttgtatt cttcttgttt ttctgggtaa
    14401 actttcatgc aaaaagcttt tttcaccatg tcagacacct gctttcgtat gaacttcctc
    14461 caaataacct gcttttggag tgacaccaaa cgcatctgct aaatgccaca ggttttcgtc
    14521 cgtgattgtt tgcaagattg gtccttgcgc tttgacgtac gtatagactt ctgctgcctt
    14581 ttctgctgtc tcgatcaaac caaagacttc atccatgtct tttcctgacc cgtagattcc
    14641 atgttgtggc cataaaacca atcgagtttc tttcattttt tcagctgttg cttccccaat
    14701 ctcatttgtt cccggaacca tccacggaat gatggaaatt ccttctggaa aaacaactaa
    14761 gcattccgta cacatttgcc atagcgtacg actaaaggaa cgttcgtcta attcatgcgt
    14821 aaaactcatc gctaatagat gactagcatg gcaatgcata acgatccgat tatctgggtc
    14881 tacttctaaa cgggcgatat gactcatgaa atgacttggc aactcgcttg taggaaggca
    14941 ttcgttttcc agcccccaca aaagttccag tgttttgcca tcttcagcaa ctcgaacgat
    15001 tccaagattc tcggcaggtt cgctcgctac atttttaaaa tatcttcctg aaccagttac
    15061 aataaaatag catcctgcta aacggctggc atcaaaaatc atcgagatgc ggcggatcac
    15121 tttttcagga tccaagaacg gtgtgatctc ttcttctttt agtaaataag aaatattccc
    15181 gccatttctt tcatcccatc ccaagcgata aagattcgac gtcgtttcga tcatttcttt
    15241 cacataaggc gcttgtaaaa tatctatttt tttcaatata gccacactcc tattttctta
    15301 attccaacac gtctttttga tattcctgga cttcttccag ccaatccaaa ccaactggaa
    15361 cttgattttt gtaacagaaa taattccata catcggcata cgggaaatct ttcaattctt
    15421 ccgtataagc cattcttgaa gtaaagtcaa aagccagctc catttctttc aatcgctcaa
    15481 ctggttccag catagctttc agcagtgctt tttgtgtatt tctcgtaccg atgacccaag
    15541 cagcgatacg gttgatcgtt gcatcaaaga aatccagacc gatatttgtt ttttccaaca
    15601 attcgttacg gactaattct ttagcgattt cctgcaattc atcatccatc aaaaccacat
    15661 ggtctgaatc ccaacgcact ggacgggaaa catgcaacat gattcctttg ctgaataaag
    15721 ctaatgaaga taatttattg gaaatcactt ctgttggatg aaaatgtcca gcatctagac
    15781 aaacaagttt gtcacgagtc aacccgtaac ccatataaaa ttcatgggaa ccgactgtat
    15841 aagcttcagc gcctaaacca aatagtttgc tttctactgc atcttgtaca taccgttcat
    15901 ctaactcttc tgtaaaaatt tcatcgagtg attccattaa acgttcccgt ggggtaaaac
    15961 gatcaatcgg attatctttc atcccatcag gtacccaaaa gttattgata cttgtttgcc
    16021 cagtttcttt cccaaagtac tccgcgattt tgcggcttct tttcccgtgt tcgatccagt
    16081 attcacgaac ttctttatct ggatgagcca atgtaaagcc gtctttcatc attggatgcg
    16141 aaaagaaagt cggattgaag tcaagaccga tttcttcttt ttttgcccac tctacccatt
    16201 tttcaaaatg tttcggctca atttcattta gatcgacaga ttcttctgta tccaaataga
    16261 tagcatgaag gttcaatttg tgtttgcccg gtatcaacga ataagccttc tccagatctt
    16321 gtctaagctc ttcaggtgtt cgtgcggctc ctggaaaatt ccctgtcgcc atgattccac
    16381 cggtcaattc gccatccggt gtcaagaaac ctttgacatc atctccttgc cagcaatgca
    16441 tagaaatttt gatgtcctgt atcttttcta gtacagcatc tgtatctaca ccgatagttg
    16501 catatttttc ttttgcttgt tcgtaatttc tttcaatcgt gttcatacga ttactccttt
    16561 gattttattc acgtaccatt ccatcgtgtt ttgcttattt tcatctgggt aaaaagtttc
    16621 acatggaaac gactgtttga ttacatttct tgcttgttct atattcgaaa actgacctgt
    16681 actgagcatt tgcatggcaa cattcccgat tgctgtggct tcactaggtc ctgcttctac
    16741 aatgattccg gcataatctg ctgtcaattg attcagtaga cgattattcg atccgccccc
    16801 tacgatgtgc agcttttcca atttcgttcc agttaagtgt tctactttga tcagctcttg
    16861 tgcgtaacaa agagcaagat tatcatacac acaacgagcg atagctcccg atgagacagg
    16921 gactggctgc tctgtttcaa aacagtattg ctgaatctct tctatcatat ttgggggatt
    16981 caaaaatcga gggtcattga tttccatcac ttgctgcagc ggtttttctt ttgcagcaag
    17041 ctctgccaat tccgcataac tgtactcgta ctcttgcatt cgggcaactt cctggatcaa
    17101 ccacatcccc atgatgtttt tcaaaaaacg caccgtgtta tgggcacccc attcattcgt
    17161 ataattttca gaaaaagcat tgcgagaaac attggcgatc tttgtttcaa tccctaaaag
    17221 cgaccatgtc ccactgctga tataacccca acgttctcct tgacctggcg ttcctaatac
    17281 agctgacgcc gtatcatgac ttgctactgt gataaaggtc acatctggca aatcatattt
    17341 tgaaaattgt tctttttgca gatctcctag tatcgttccc ggctctgtca atggtggaaa
    17401 taattccttc gagacaccga ttttttcaag aagttctatt tcccattctt ttgtccctgc
    17461 attcagcagc tgcatagtag atgcattagt tttctccgtc accatgttac cagtaaagac
    17521 ataccctaaa taatcaggta tcagtaacaa tttatgtgct tttgccaaat tttctttttc
    17581 ttcagcaaac aattggaaca aggtattgaa aggctggatc tgaatccctg tctgctcata
    17641 caacttatct agagaataga tttcagaaaa ctgttctact gctccattgg tacgcttgtc
    17701 ccgataagca atcggatcat ttatccgatc cccttgttca tcaatcagac agtaatcgac
    17761 tgcccatgta tcgatccccg caaaaaggcg atcaatccct gtctgcttta atttttcaaa
    17821 tccaatcaaa atctctttga taagataatc gatcttccag cgatcgtgac cgtctttgtt
    17881 ccgaaagccg tttttgaacc ggtgtacttc ttccaagatc aaagaacctt ttgcatccct
    17941 cgtacttttc attagtcgtc cgcttgatgc accgatatcg atggcaacaa atgttggatt
    18001 catttctcca cctcttttac tttattgttg attagcactt tattaataaa gcgttatcat
    18061 ttattgacaa tgctatcata atacagaatc ttatattttg cttcacacta ttttggtata
    18121 taattgcatt atcttgacaa gttgggagtg tttacatgga ttactgggaa aaagccaacc
    18181 actcatggtc ggaagactca aaaagagtga tcttgacgcc tacacaaaga agcaggaatt
    18241 tattttttta tatccaagaa atcggttact tcaaagcacc aatcccttac tatactgaac
    18301 gagcacattt accttcttac ttgataaagt ttactttgtc tggcaaaggt tcgcttacat
    18361 ataatgataa tcagcaacag ctaaaagctg gagacttatt ttttattgac tgccaagaat
    18421 atcagcgtta tcaaacgatc agcgaagaac catgggaaat ggattggatc catttgaatg
    18481 gtcagaacgt ccaatcattt tatcaggagt tcacaaaaga cggccataac accttccatt
    18541 caacaggaga tccatcagaa aacaagatcc accatttgat cacagaattg atccacatgc
    18601 aacaaggcac gaatgcccgt accgatttcc agtcctctgt agctatccac gagttgctga
    18661 atgaactgat tttgcaaaag tatcagctag atttcgaaga agaggatatt cccgcctacg
    18721 tttacaaact gaagcaatac ttagacgaac atttcaaaga aacgattaca cttgatcaat
    18781 tagaacaaca attcttattg aataaatatc agctgagcaa agatttttcc aaatttatcg
    18841 gttctccacc aatcgattat ttgatcagca aaaaactttc ttacgctaaa gaccttcttc
    18901 ggtatacaga atttacggtt cgaatgattt ccgaagaaat agggattgag aattttgctt
    18961 actttagccg cctgttcaaa gcaaaaacag gactatcgcc gagagacttt cgtaa
//
LOCUS       DARDHA010000041        18999 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000041 DARDHA010000000
VERSION     DARDHA010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.041"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            37..615
                     /locus_tag="SDA10_001162"
     CDS             37..615
                     /locus_tag="SDA10_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG4240086.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            615..1544
                     /locus_tag="SDA10_001163"
     CDS             615..1544
                     /locus_tag="SDA10_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4240087.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            1541..3277
                     /locus_tag="SDA10_001164"
     CDS             1541..3277
                     /locus_tag="SDA10_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG4240088.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(3340..4176)
                     /gene="pheA"
                     /locus_tag="SDA10_001165"
     CDS             complement(3340..4176)
                     /gene="pheA"
                     /locus_tag="SDA10_001165"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEG4240089.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(4173..4682)
                     /locus_tag="SDA10_001166"
     CDS             complement(4173..4682)
                     /locus_tag="SDA10_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEG4240090.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(4675..5970)
                     /gene="aroA"
                     /locus_tag="SDA10_001167"
     CDS             complement(4675..5970)
                     /gene="aroA"
                     /locus_tag="SDA10_001167"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEG4240091.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(5983..7074)
                     /locus_tag="SDA10_001168"
     CDS             complement(5983..7074)
                     /locus_tag="SDA10_001168"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEG4240092.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(7091..8257)
                     /gene="aroC"
                     /locus_tag="SDA10_001169"
     CDS             complement(7091..8257)
                     /gene="aroC"
                     /locus_tag="SDA10_001169"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEG4240093.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(8259..9323)
                     /gene="aroB"
                     /locus_tag="SDA10_001170"
     CDS             complement(8259..9323)
                     /gene="aroB"
                     /locus_tag="SDA10_001170"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEG4240094.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(9356..10378)
                     /gene="aroF"
                     /locus_tag="SDA10_001171"
     CDS             complement(9356..10378)
                     /gene="aroF"
                     /locus_tag="SDA10_001171"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEG4240095.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(11037..12011)
                     /locus_tag="SDA10_001172"
     CDS             complement(11037..12011)
                     /locus_tag="SDA10_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HEG4240096.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(11992..13875)
                     /locus_tag="SDA10_001173"
     CDS             complement(11992..13875)
                     /locus_tag="SDA10_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEG4240097.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(13893..16118)
                     /locus_tag="SDA10_001174"
     CDS             complement(13893..16118)
                     /locus_tag="SDA10_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEG4240098.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            16233..17267
                     /locus_tag="SDA10_001175"
     CDS             16233..17267
                     /locus_tag="SDA10_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4240099.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(17334..18347)
                     /locus_tag="SDA10_001176"
     CDS             complement(17334..18347)
                     /locus_tag="SDA10_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG4240100.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(18425..18613)
                     /locus_tag="SDA10_001177"
     CDS             complement(18425..18613)
                     /locus_tag="SDA10_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEG4240101.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         5331 a         4107 c         3476 g         6085 t
ORIGIN      
        1 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
       61 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
      121 tttcaattaa tagggacttt ttccagtccg atgaaagcgc ttgatttttc tttggtacat
      181 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
      241 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
      301 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
      361 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
      421 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
      481 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
      541 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
      601 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
      661 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
      721 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
      781 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
      841 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
      901 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
      961 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     1021 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     1081 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     1141 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     1201 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     1261 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     1321 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     1381 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     1441 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     1501 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     1561 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     1621 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     1681 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     1741 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     1801 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     1861 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     1921 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     1981 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     2041 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     2101 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     2161 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     2221 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     2281 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     2341 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     2401 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     2461 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     2521 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     2581 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     2641 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     2701 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     2761 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     2821 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     2881 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     2941 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     3001 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     3061 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     3121 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     3181 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     3241 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     3301 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
     3361 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
     3421 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
     3481 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
     3541 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
     3601 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
     3661 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
     3721 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
     3781 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
     3841 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt
     3901 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
     3961 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
     4021 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
     4081 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
     4141 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
     4201 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
     4261 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
     4321 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
     4381 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
     4441 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
     4501 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
     4561 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
     4621 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
     4681 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
     4741 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
     4801 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
     4861 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
     4921 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
     4981 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
     5041 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
     5101 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
     5161 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
     5221 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
     5281 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
     5341 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
     5401 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
     5461 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
     5521 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
     5581 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
     5641 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
     5701 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
     5761 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
     5821 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
     5881 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
     5941 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
     6001 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
     6061 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
     6121 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
     6181 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
     6241 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
     6301 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
     6361 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
     6421 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
     6481 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
     6541 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
     6601 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
     6661 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
     6721 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
     6781 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
     6841 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
     6901 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
     6961 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
     7021 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
     7081 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
     7141 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
     7201 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
     7261 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
     7321 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
     7381 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
     7441 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
     7501 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
     7561 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
     7621 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
     7681 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
     7741 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
     7801 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
     7861 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
     7921 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
     7981 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
     8041 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
     8101 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
     8161 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
     8221 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
     8281 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
     8341 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
     8401 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
     8461 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
     8521 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
     8581 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
     8641 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
     8701 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
     8761 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
     8821 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
     8881 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
     8941 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
     9001 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
     9061 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
     9121 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
     9181 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
     9241 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
     9301 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
     9361 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
     9421 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
     9481 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
     9541 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
     9601 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
     9661 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
     9721 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
     9781 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
     9841 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
     9901 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
     9961 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    10021 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    10081 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    10141 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    10201 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    10261 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    10321 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    10381 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    10441 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    10501 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    10561 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    10621 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    10681 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
    10741 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg
    10801 taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt tatgaaactt
    10861 tttttaattt tctgatagtt aactttgttt ttcagaaaaa taacaattgg ttttattgaa
    10921 gaaaatagtc atgaacaaaa gtatctgaga acttttttac tctcttgttc atgacatata
    10981 gaataaagca gctttgtctt gtaaactccg tttttcaaat aataatctaa agattgctac
    11041 tgaataataa agccgatttc atggtcctct tcgctgctga ttcggtaagg tgactcaaca
    11101 tccgctcccc agctattgat acccccgact cctcttactt ttgcaaaaat cgtaagaacc
    11161 gttcgtcttt ctggcggtag ttcttctata tgtgttgcct gttccagttc ttctgctttg
    11221 aatggtaaag cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct
    11281 tcttgatctt tatccgtatt atccaacgtt tgatttcttg ttagttctac ccatttcgtt
    11341 tccatatgca tgccgcattc ttggggaaca aggtatgggg aaacggggag tcctgccact
    11401 ttataaatac cttcgacacc accagccatc cgatccggat atgtttcccc tgataggcct
    11461 tcatactcaa aaaattttgc tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat
    11521 tccggtaatc ctttttgtcc ataataatgc atattgacaa gcattttacc ctctccagcg
    11581 atttggtagg aaattgtcac ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg
    11641 atttcaaccg tttcagcgta ttcattactt gaatattgat tatttactgg agcaatcggc
    11701 aaatcgatct ttttattgtc caccagtact tctatttctt tgacttttgg atacaatccg
    11761 gctcctaacc acataccgga tttaaatcca aatccgctac ctctgtcatt atcggtcgtt
    11821 gctcgccaaa aaactggaag tgtttcccgg tatagccatt cttttccgtt tttattgagc
    11881 gattccagtc cgcctcttgt ataagaaaaa atataatgcg tattccccga tttgacacct
    11941 aatgtacagt cgccgaagac gattgcaact ttttccgtat ccatatgact tttatttact
    12001 gtatttttca taataatatc ttacctcctg catggcgggt ttttcttgtc catctgcaaa
    12061 aacgatccca ttccctgaga attcatagtc acttggttta tcgtcaaagt caccgccata
    12121 gcgcatcact tctctgcctg tgattggatc agtcacttgg atcgcctgat ctttaaaatc
    12181 ccatatatag cctccttggt acatttcata acgatcaagc aacgtcatgt aagaatccat
    12241 tccacctaac gaattcccca tgtcatgcat atattcacat aagatgaacg gtttttccgc
    12301 tttattttca aggtagtcaa tgatctcttc tggtggcgca tacatgcagc tttttacatc
    12361 tgaaatcgaa tcttcgaatg ctggatttgg aaaattccct tcataatgga caagtcgatc
    12421 ggggtctact tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg cataagattc
    12481 atttccaaga gaccaaaaaa gaatacttgg atggtttttt aatgtttcga aattcgtttt
    12541 tgctcgatca agaacagccg cctgccactg aggtactgca cctggtacgt tgtaagaagg
    12601 ctcaaatgcg cccatttttt gccatgagcc atgggattcg aggttcgttt ccgccatcac
    12661 atggattcct gcttggtcgc ataaataata ccaagtgatc tggttggggt aatgagatgt
    12721 ccgtacgccg ttgatctttg cattttttat gatagccaaa tctttttcca tatcttctat
    12781 agtgatggcg cgtccttttt tcggatgcca ctcatgtcga tttaccccgt tgataatcat
    12841 ccgttttcca ttgaagtaga tgattttatc tttgatttcg attttacgaa aaccgaagga
    12901 atagtcaata tactccgtta ctcttttttc ttcgtctatc gtctccaatt caagcttata
    12961 taaattgggc tgaaaatgcc cccacagtgc cacattttca aatccatcga tgcgaatcgt
    13021 catttgctct tttaacggat attgctgttg atagatttcc ttcccatcag gatctagtat
    13081 cgctacattc agctgactgt ttttggctac ttgctcaaaa gacaaatcta cttcaagttt
    13141 gccattttga catttgtcat ctaagcgagg tttgatccaa agatcttcta aatggttttg
    13201 tcctcgagca actaagaaga catctctgaa gatcccaaaa aatcggaaaa agtcctgatc
    13261 ttccaagaat gcagctgtac ttcttttgta aacctccaca gctagaatat tttcttcttc
    13321 caaataaggt gttaaatcaa attcagacgg agtgaacgaa tcttcggcat acccgataaa
    13381 atgaccattc aaccaaataa acattgcttg ttctacacca gcaaaatgaa tcgagatttg
    13441 ttttcccttt agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc
    13501 tttagcagct gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga aagggggacg
    13561 gcgaaataca tgtccctccc acggatacat cgtattgatg taatggatct tgtcataatt
    13621 tgctaattca atatgactag gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt
    13681 tttataaaat gtttttggtc gtaccgaagg atttggcgaa taacaaaaac gccatgatcc
    13741 atttaaagac tgtttaaggg aggatgtggt ttctacttct tcccttgttg cataacaaac
    13801 atgatcgcta tgtgcatttg attggttcac tctaaaaact tgtggatcat ctaaccattt
    13861 tatatttgct ttcatacttt tttccttctc tctcataact ttttgaaatg aagcagttct
    13921 gtttggaaat caccttgaaa atcggggaag taaagtccaa ctgtcatcaa ttcggccccc
    13981 gaaaatatcc gcttcgaggt ctgttcctga taaagtgcct catcatctaa ccccatcaga
    14041 tatacttcat ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa cacaatagct
    14101 tcatcttgtt taggagacac aaacatccat gcggcaatat tttcttcaaa aggactcgcc
    14161 aaacgataat aatggccata ctggatcacc ggacgaatcg tttgatagga tataatttgg
    14221 tcttttactt gctttttttc ttcaggagac agctttgtca aatccagttc ataaccaaaa
    14281 acagcgctca tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga
    14341 tttggtacag ctgatacatg agctgtgatg gacgaaatcg gataagcaag gcttgttcca
    14401 tattggattt tcatacgctc cactgcatcc gtgttatcac ttgtccatga ctgcggcata
    14461 taataaataa acccggcatc aaaccgtcca cctccaccag aacagccttc ccataagatt
    14521 tccggataat cagttgtcaa atgttcaagt agttcataaa gcccgagcat ataacgatgc
    14581 gctacttctc cttgtctctg tgggtcaagg gtaatcgaat agacatctga aagcgaacgg
    14641 ttcatatccc atttgatgta gtcaagagga atcgtatcta aaatctttct catttgtttc
    14701 tcaatcgtct gccgaacatc taaccttgta aaatccagta catgttgtga tctggaagca
    14761 gaaggcattc tacctggctc ctgcataaga aaatcaggat gtgttcgata cagttcagaa
    14821 tcaaccgaga tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt gctatgagca
    14881 taatctgcta tttcacgtaa tccatttgtc aacttgcctt catactcgaa ccagtctcct
    14941 aatgaagaat tgtccgcatc tctttttcca aaccagccat catctaatac gaacatttcg
    15001 atgcctagct cctttgcttc atcaatgatc gccttgattt tttcactatt gaagtcaaag
    15061 taagtggctt cccagttatt gaccagaatc ggccgttcag caaattgatg acgacctctt
    15121 gcgattcttt cacgtagcaa ctcatggtgg atctgactca ttttgttcaa tccctgactt
    15181 gagtaagaaa ggatcatttc aggtgtttga aaagactggc ctgcattcaa ttcccacgaa
    15241 aaacgatggc tgttgatccc accgacgact ctcaattgat tgatctgatc tttttcaagt
    15301 tcgaaagaat ggttgccgga ataaacaaat tgaagtccga tcgcttctcc agtattttct
    15361 gtcgtatgat gatgaactaa agcaataaag ttattcatat ggtgactgct cgttcctcga
    15421 cggctttcaa atacttttgt tccagataaa acagattgcc gtgaaatctg tctttcattg
    15481 acatgtgctc ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc aaagtctagc
    15541 tggaaactag ccgctttttc tagtttgatt gtttctccgg tttcatttct tatctttaca
    15601 gatcgtgtga tcactgctct atcagtaaat atggtataga acaaatcgaa ataaatcgct
    15661 gtttctctat ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc catcacataa
    15721 gtttgaggca agccttcgat ctctggcttc ccatctgtaa tataatggct gtcatatctc
    15781 agatccagta agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc
    15841 atgctgccat gagaagagta ttcttgcggt agtgtatctt tcgacagaga ccggtcagga
    15901 tttaagggaa catttcctga aaatccacga tctcttcggg gataattttt attgtctttg
    15961 tactgcttga ctggtttgcc aaaataaata tgcgccaata cattgctttc ttctatcttt
    16021 atcagataag aaagaaatgt atttcgtaaa tggaagactc ggctcttttc atcaaataag
    16081 atgaaatttt tatctgtttt tctatgcgta atatccatca tttcactccc aacaaaacgg
    16141 aaaccgttta ctttttttta taattcctgt atactgttta ctaaacaatt ttgtcaataa
    16201 aaatttttta tttttttgag gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa
    16261 aggcaaatgt ctctcctgct acagtttccc gtgtgttgaa taacgatcag acgatccatg
    16321 tggctcagac tactcggcaa cggatagtag aggcagctgc tgcgttggat tatgacatca
    16381 gtactcgtaa atatatcaaa aagagaatgc cttcgattgg tgtgatctct acgatcggca
    16441 aagcgagcga agaacaggat atctattaca aacatttgcg agtagggatg gaagaagaag
    16501 ctcgacgtct gcatttaggg atgaatcgaa tctataatct atctgataat cctaaacaat
    16561 gggaagatct agatcagtta ggtgcggtga tcgttgttgg gactgttacg aaagaatcga
    16621 ttaccgcctt gttaaaacaa aacatgcaaa tagtcgtcgt ggataaccct gacatccagc
    16681 aagaggtcga tatggtgtat gcagatcttg aacggatgac taaaaccgta ctggaattgt
    16741 tcttagaaaa tggtcataaa agaatagcct atatcggggg atataaagtc gatatggatg
    16801 aattcggtgg gaagcagatt tctataaatg aaaagaggat gcgtgcctat cagcatttta
    16861 tgatcgatca tcacttagaa aaatatatgt cttatcatct aggtggctgg acagaaaaac
    16921 aaggtgaggt acttgcgact aatctattag aaaatgaatc ccctcttcct actgccattc
    16981 ttgtcgggag cgatccaatg gcaattggtg tctatcgggc attgaaacaa aaaaatatcc
    17041 aaattggaga agagattgtt gtagcaagct ttgataatat cgagacaatc gcaaacctct
    17101 cccctggctt gaccagtgtt caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa
    17161 tggcattgga gcgaattgac ggtatacggg acgaaccaat cattttgact tatcctgcac
    17221 gattgattgt tagggaaagt tttatcccaa gaacaaaaag gtattgaaag agaggttgtg
    17281 aaaagccgct tcgtttcttc atactcaaaa cgaaacggct ttttcacaac ctcttactta
    17341 tacttattca caaaatccaa tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa
    17401 tcaaattggt attcatgggt aatctctttt tttgtatctg tataaaacaa ggattgcacg
    17461 ggtaccttta attctgtcaa ccgattttct aatgcttgac cttgctcctg gaatgagtat
    17521 gcatttccgt cagttatata cgttggcgga aattccttag aaacatggtc agccacacta
    17581 gcttctttca attgctcact cgttttccaa tcttttgttc caatcaacga ccacgcaacg
    17641 gttttgacga aaaacttcat aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa
    17701 tcaacaggtc cccaataaga aatggttcct ttcagactat cttttggaag tagctgtttg
    17761 attttcattt gtttagcata cgcatcattc gtttgtatag ctgcatattg caatgcgatt
    17821 tgtgaacctg cactatctcc accaaaaaat aattgttcca tgtctagcaa tttgtcgtct
    17881 ttttcttcag ctaataaatc tttgatcagt tcattcactt gaagaacttg attagggtat
    17941 tctgaatcag gcgctaattc ataattcatc gcaaccactg cgatgttggc gtctgaaact
    18001 aattttgtcg caaattcttt gacacccgat ttatctccgc cgacgaatcc tccaccatgt
    18061 acccaaaata gaacaggaac aggccccttg ctgttttttg gatagtagat gtcataagta
    18121 ttctctttat acttggactc atacgtcaaa tctgtttttt tcgccacgtt tttttctgct
    18181 tgatcgaacg tttttttatc agtgatttga acagtatttg aaaacatatg gttgatcaag
    18241 aatgctccag gtctggggga aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga
    18301 atgattccga agaaccatcc gataaattta agaattttct ttttcataag gctccctcag
    18361 tagttagtat taagcattct gattatacca tagtgttatt tcctatcaag ctctcctaga
    18421 tgatttaatg attttcccca aacttcttct gcaatgcttt tgacaaaaga aattttttcc
    18481 attgttcttc aaacaaaaca ttttcttctt ctgttgaagc aaatccacat tgaggactta
    18541 agcacaaacg atccaaaggc agatatcgtg tcgcttcgta gatccggtca atcacttgct
    18601 ggtgctcttc catttcgcca tttttcgtta cagtcaaaat ctaagacaac taatttatct
    18661 tcagagactt tctctaaaag aacaaaactc ccagcacgtt cactatcgta ttctaaataa
    18721 taggcacgga cattttcttg agtaaaataa tgggtctgct acgtcgtcat cccccctgat
    18781 gccgccaaaa tcgaataata atttccgcgt caaacatgta tatttagtgt tgaatcattt
    18841 gagaatatga aaataaaaat ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa
    18901 atctgagaca gaatgacgtt cattctgacc cagatttgat tttgcacaac tcaacagtcc
    18961 ttattggcaa atcgccaaaa taatgtagac taactaaaa
//
LOCUS       DARDHA010000042        18137 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000042 DARDHA010000000
VERSION     DARDHA010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.042"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..152
                     /locus_tag="SDA10_001178"
     CDS             <1..152
                     /locus_tag="SDA10_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4240102.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="SDA10_001179"
     CDS             complement(230..763)
                     /locus_tag="SDA10_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEG4240103.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="SDA10_001180"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="SDA10_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEG4240104.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="SDA10_001181"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="SDA10_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEG4240105.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="SDA10_001182"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="SDA10_001182"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEG4240106.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="SDA10_001183"
     CDS             complement(4488..5066)
                     /locus_tag="SDA10_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEG4240107.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="SDA10_001184"
     CDS             5388..5858
                     /locus_tag="SDA10_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEG4240108.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="SDA10_001185"
     CDS             complement(5966..7192)
                     /locus_tag="SDA10_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG4240109.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="SDA10_001186"
     CDS             complement(7946..8146)
                     /locus_tag="SDA10_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG4240110.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="SDA10_001187"
     CDS             complement(8280..8888)
                     /locus_tag="SDA10_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEG4240111.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="SDA10_001188"
     CDS             complement(8973..9443)
                     /locus_tag="SDA10_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEG4240112.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="SDA10_001189"
     CDS             complement(9491..10405)
                     /locus_tag="SDA10_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG4240113.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="SDA10_001190"
     CDS             10551..11354
                     /locus_tag="SDA10_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEG4240114.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="SDA10_001191"
     CDS             11532..12479
                     /locus_tag="SDA10_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEG4240115.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="SDA10_001192"
     CDS             complement(12547..12840)
                     /locus_tag="SDA10_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HEG4240116.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="SDA10_001193"
     CDS             complement(12868..13533)
                     /locus_tag="SDA10_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEG4240117.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="SDA10_001194"
     CDS             complement(13673..14305)
                     /locus_tag="SDA10_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4240118.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="SDA10_001195"
     CDS             complement(14312..15379)
                     /locus_tag="SDA10_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG4240119.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="SDA10_001196"
     CDS             complement(15376..16107)
                     /locus_tag="SDA10_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEG4240120.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="SDA10_001197"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="SDA10_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEG4240121.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="SDA10_001198"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="SDA10_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEG4240122.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         5378 a         3682 c         2904 g         6173 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgat
//
LOCUS       DARDHA010000043        18079 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000043 DARDHA010000000
VERSION     DARDHA010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.043"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(193..513)
                     /locus_tag="SDA10_001199"
     CDS             complement(193..513)
                     /locus_tag="SDA10_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240123.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(558..701)
                     /locus_tag="SDA10_001200"
     CDS             complement(558..701)
                     /locus_tag="SDA10_001200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240124.1"
                     /translation="MKFRLISEEATSGTPFKKEVVTWSRPLFDDYDTKNIIYEQNVNN
                     GRS"
     gene            complement(1016..2134)
                     /locus_tag="SDA10_001201"
     CDS             complement(1016..2134)
                     /locus_tag="SDA10_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240125.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            2605..3912
                     /locus_tag="SDA10_001202"
     CDS             2605..3912
                     /locus_tag="SDA10_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEG4240126.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            4033..5232
                     /locus_tag="SDA10_001203"
     CDS             4033..5232
                     /locus_tag="SDA10_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEG4240127.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(5259..6527)
                     /locus_tag="SDA10_001204"
     CDS             complement(5259..6527)
                     /locus_tag="SDA10_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEG4240128.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(6697..7338)
                     /locus_tag="SDA10_001205"
     CDS             complement(6697..7338)
                     /locus_tag="SDA10_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HEG4240129.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(7747..8250)
                     /locus_tag="SDA10_001206"
     CDS             complement(7747..8250)
                     /locus_tag="SDA10_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG4240130.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(8373..9137)
                     /locus_tag="SDA10_001207"
     CDS             complement(8373..9137)
                     /locus_tag="SDA10_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEG4240131.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            9245..9520
                     /locus_tag="SDA10_001208"
     CDS             9245..9520
                     /locus_tag="SDA10_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEG4240132.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            10080..11030
                     /gene="yidC"
                     /locus_tag="SDA10_001209"
     CDS             10080..11030
                     /gene="yidC"
                     /locus_tag="SDA10_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEG4240133.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(11076..11477)
                     /locus_tag="SDA10_001210"
     CDS             complement(11076..11477)
                     /locus_tag="SDA10_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG4240134.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(11491..12300)
                     /locus_tag="SDA10_001211"
     CDS             complement(11491..12300)
                     /locus_tag="SDA10_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG4240135.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(12287..13177)
                     /locus_tag="SDA10_001212"
     CDS             complement(12287..13177)
                     /locus_tag="SDA10_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4240136.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(13190..13669)
                     /locus_tag="SDA10_001213"
                     /pseudo
     CDS             complement(13190..13669)
                     /locus_tag="SDA10_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(13845..14786)
                     /locus_tag="SDA10_001214"
     CDS             complement(13845..14786)
                     /locus_tag="SDA10_001214"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HEG4240137.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(14783..15778)
                     /gene="lacD"
                     /locus_tag="SDA10_001215"
     CDS             complement(14783..15778)
                     /gene="lacD"
                     /locus_tag="SDA10_001215"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEG4240138.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(15771..16967)
                     /locus_tag="SDA10_001216"
     CDS             complement(15771..16967)
                     /locus_tag="SDA10_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG4240139.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(16985..17713)
                     /locus_tag="SDA10_001217"
     CDS             complement(16985..17713)
                     /locus_tag="SDA10_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4240140.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
BASE COUNT         5434 a         3844 c         3085 g         5716 t
ORIGIN      
        1 gatgttcttt tttaaactaa aaaaattatt ctttctgact acttatcggt tctgtttcaa
       61 cggtcgaggc tggggaagat tctttagtag gtctgatgtt ctcatggtat ttctgatagc
      121 ctatgacagt taccagaaga acaaggcaaa ccaatacgac caacgagatt agtttttctt
      181 ttttgctcat atctatacct cataaataac aggatgatgt ccatcatatt tctgagtatg
      241 agtaaaagca aaagctgcag ctgtagaatg taagaaatgc cagcaacttt cataatcaca
      301 ggtcggacag taccaaaagt aaccaaatct tggctgcaaa gaccgttctt ctgtttcagg
      361 aatcgcagaa tcaagatctg gtgtctgtac ataaacattt ttgtacagac gtgtgcctgt
      421 aataggaaac cgctgtgttt cttcagcaaa aactggctga cttgttaaac ttagggatag
      481 agacagcaaa caccataaca gtcttttttt cataaagacc tccttatcga taagtttttc
      541 ccaattaaaa tcatatctca agaacgcccg ttattcacat tttgttcata aatgatgttt
      601 tttgtatcat aatcgtcaaa aagaggtcgt gaccacgtca caacctcttt tttaaacggt
      661 gttccagaag tagcttcttc ggaaataagc cgaaatttca caaaaatttg agaaataatt
      721 tttgtcaatc tcttcttatt tcttgaagct gaccacttct gtcacaacct cttccttaaa
      781 cggtgttcaa aagcttgcat ctgcggtaat aagcccacca aacaaaaaat atgaagagct
      841 atttttgctt gctgttctta ttacttgatg cagaacagct ttttcacaac ctctcataca
      901 cggtgtttaa tcagctgacc ttcggtatta gctcaaaaaa cggcaaaata tgtgaaacta
      961 tttttccgtt tgtttctcta atactcaggt caaaacactg atttcacaac ctcttttaat
     1021 aacgaagaat cgctgtcaga ctcttttcat ctggcgcatc tttttgatcc aaaataaaca
     1081 cttttccgcc attaatgatc acatcatctg ctaatgcatt caacacttga cgccgatcat
     1141 attctgtttc aggatcttca ccgaaaccat cagctaagtt tgacgtagcg ataaataaat
     1201 gtgacacttt tccttctttc gcagctggaa cgatatcaac taactgatcg atgaatttct
     1261 tatctaagag tttattgtat tcttttgatt cttgagctgc gagttcactt gacagcttct
     1321 tcgccccttc ttcgatatct ctaaaggata gctgcgcagg agaagcagaa atcgcaacgt
     1381 ctgcacaata atagggattt ttagcaattt ttttgaacat cgtctgattt tctggtagag
     1441 caaataaata aatcggccat tgttctggat tttcgaaaga atccttcaag tacgtatcaa
     1501 ctgcttggta atagttgacc caatcgatct ctacttcttc atcttttgga tttacgccat
     1561 ggaaagttac tccatctcgg gctccgcctc ctccattaga agagaagttc aaaccgccac
     1621 ctgtcagttc agatcccaat gcagtaacga catcaaccgg tgccccttct ggtagagata
     1681 cttcttttac ttgtttgttg ctgacttcat acaatttcat ggagtcgcgg ttcaatccca
     1741 ataaataata agaatagttg aactgcccat ttttgataat agccagtaaa taaggaagat
     1801 cacttacata gtattgatcg tctaccggga tactcaaacg atggatatag gtttggtcag
     1861 cagttataat gaccgctaca ctcgtagttg cactacgcca aaacgaagga tctcctaaca
     1921 aattttcaat tttttcttgg aacggggacc agtcatgttc ggaatatttt ttctcaaaac
     1981 gtttctttgc ttctttcgca aaatttttga atttgatctg atctttttcc acatcttgat
     2041 gggcgacatg cgtattcagc atgatcgtga cgaatggtcc tttagcgcct tctgaagtca
     2101 attgtgacaa taattcttta tccttttttg ccataaacgt acccctccta aaaaaatcat
     2161 tcctttgttt ctatctaaag tctaccacaa cactattttt ccatacaata aaacgtatct
     2221 aaaaaaataa aacggtttct acggttttaa atttagctat ttttttaagt tatcttcata
     2281 atttcctcaa ttatttagcc ccagtcttca actattcttc atgaaatgct gataaactaa
     2341 gaaaaagaat aaaagaggtg gatcttatgc atttgattat gattttcagt ttattaagta
     2401 ttatcagttt atttgctttc agcgcttccc gcttattaga tgcggaaaca cagcctattc
     2461 ctgtaaaagt acgtaaaaac gaccgctaac cttccataac gtgcgttctt atcctaaagt
     2521 tttttcagaa acattgaaac tctctcccct ctctgataaa attttaattt gtagcgaaac
     2581 aaattaatta caaggagatc ggatatgtcc ataaaaaaaa cacgtctagt cctattattg
     2641 gctagtctca ttctttgcct tgcttctttt ctaccagctg ttgctgtgca agctgaagat
     2701 actttcaaag taaatgcaaa agcagccttt gctgtagatg cgcaaagcgg taagattctt
     2761 tatgatcaag atggagaaaa aaccatgggg atcgcttcaa tcacaaaaat catcggtctt
     2821 tacatcgtcc ttgatcaagt aaaagaaggg aaactatcat gggatgataa agtttcaatt
     2881 tcagattatg cagaaaattt gagtgtcacc ccagatttat caaatgttcc tttgcataaa
     2941 gaaaacacct acaccgttaa agaattattc gactctgcaa tcatccagtc agccaacgct
     3001 tcaatggttg ccttggcaga aaaaatctct ggcagcgaag caaaattcac tgaacgaatg
     3061 aaagaacaat tgaaagattg gggtatcaaa gatgcaacga tcgttaatgc atcaggactg
     3121 aataactctt atcttggcga aaaccgacca gaaggtactg gagaaaacga cgaaaatcaa
     3181 atgtctgctc aagatgtggc tatcgttgcc cgtcatttga tcttggattt tccagaaata
     3241 ctggatgtat ctagcactac gacacaaatg tttggcgaaa acacgcaatc accagtagaa
     3301 atggtcaact ggaactggat gctcccagga ttcgtgaatt ataaagaagg cgtggacgga
     3361 ttaaaaactg gtacgactga acttgcagga gcctgtttcg tcggtacgat cactaaaaac
     3421 gatcaacgaa tcatcactgt cgtattgaat gcagacggcc atgcggaaaa cccaagtgcc
     3481 cgtttcaatg aaacagctcg cttaatggat tattgctacg acaattggtc agaaaaagag
     3541 cttggaaaag ccaatgcaag tatccctagt ctaaaaacca ttgatgtaaa agacggaaaa
     3601 gaatcaacag tgcccgttgt gcttaaaagt cctgtaaaag tatgggtacg gaacgatatg
     3661 gatactggaa aattaacaat cacaccaact cttgacaaaa aacaagtcac tgacaatgag
     3721 atccaagcac ctatggataa aggaacaaaa atcggtaccg caacgatcac cttggcagaa
     3781 gataaattag gttatcttga agacaacaac gcgccatcca cagaaatcat cacggataaa
     3841 accgtagaaa aagcaaatat tttcgttatt ggctggcgac gcgtcgcaga atttttcggt
     3901 aacttgtttt aattgaacaa aaagaaaaaa gcatctttcc atgtggaaga tgtttttttg
     3961 ctttttctag tttaaataac agcttttttc atctattttc gttatactgt ataagaaaaa
     4021 ggagttgaat ttatgaatct aaccagagat ctgatttttt tggttctcta tcttattggg
     4081 gtattctcca tcgtttattg gcgaaaacag aaaaaggatc cttccttgtt cttttacagc
     4141 agtacatgga cgtttgcggt aatcttgact tattttgtcg tgttggaaat tttttattcg
     4201 accttttatt ttttgattcc cttagtgttt ttcagtatct ttgccttttc ctatttttca
     4261 gaaaagcgaa ggctgttgaa tggtctttta ttcaatattt tcctcattag ttttggtatc
     4321 tacttgtttg tcttgctata cgaaacacaa aatatttttc ttggcggact gattgccttg
     4381 atcacgatcc cgttactgct tgtctttcta ttcggcattt atggattgat cgtttttctt
     4441 ttttggaacg gtgtcacagt tttacgtaga gagtcgcatt cattagctaa tcttttaacg
     4501 ttgatactag ccatttttct tacattgttt cttgtttttg actttttctt gttaaaatat
     4561 ttaccacaat ggataaatgc tttgtttttc tgcatcccat tgatcttgat ctatctattt
     4621 atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg ccctcgatac
     4681 aaccaagact tcatcgttgt gttaggcgcc gggttgatca atggagaaac agtttctcct
     4741 cttcttgcca agcgaatcaa taaggcgatt gctttttatt gggcacaaag ccgtgcgaca
     4801 ttaaatccgc ctatcctatt aatgtcgggt ggacagggag cagacgaaaa agtaccagaa
     4861 gcaatggcga tgaaacaata tgccatggaa caaggaattc ctgaaagaga tattcttgtc
     4921 gaaaccaatt cgacaaccac tttggagaat atgcgctatt caaaggaaat catggatcag
     4981 caaatgaaag gtccctatcg cgcgatcttc agttccaaca attaccacat tttcagagct
     5041 ggtctctatg cacgacaagc gaaactgaag gcaaatggca ttggggcaaa aacagctttc
     5101 tattacttgc ctaacgcgtt tttaagagaa tacatcgcca tcttgatgat gaacaaaaaa
     5161 agacatatga tcatttgcgg attgcttttc gcaggttccg cattcttatc actgatcact
     5221 ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt tttttctttt
     5281 attctcttga cgattggcaa gaatagtatt ccttttttga cggctacttg ctgtgcagaa
     5341 taaataccgc tgtttcctga acacataaag ctgatcaagc agatcataaa gaagtaagca
     5401 gcagcttgtg aaccaaataa ttcgattccc atcacaaaac aggcaatcgg tgtatttgtc
     5461 gcaccagaaa ataccccgat aaaacctaat ccagcaagaa aaggaacaga aacatggagc
     5521 aaaggcgcta gtactgcgcc aagcgttgca ccaatctcaa atagtggtgt cacttctccc
     5581 ccttggtacc ccgcacctaa agacaagacc gtgaagaata atttcccaat aaaatcataa
     5641 gcatgtgcct gtccattaaa tgattcctgc agtaacggaa gactcaaccc aaggtaacgt
     5701 tgtgtttgca aaatcaacgc tgcggctaca actactgctc cacctatcgt atttcgccaa
     5761 acaggatgag gaatccattg cgcataccgc gcttttaaaa agacgatcga acggctgaat
     5821 acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa cagcaacata
     5881 ctccattcag gaatcacacc catttggtaa tgtagatgag aaacaccata gctttctgtc
     5941 atgaaatttg caaaaagcgc agcaaagaag ctaggaaata ttgcttctgt tcggacttta
     6001 ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc aaatacagaa
     6061 ctaaaacccg cactgattcc actgataacc aaaatctccc gttctgcttt atccaatcgg
     6121 aataaatgcg ctatattatc agctaatgct ccccccattt gaactgctgt tccttcacga
     6181 ccaacagatc ccccaaataa atgggtagtg atggtcccaa acaaggtcaa tggaatgagc
     6241 cgcaaaggga tcttctcttc tcctccattc ccttgatcga tcactaagtt attcccacga
     6301 ctagaaagac cgccatgata cgcataaaga taagcaaaaa gcgcgccgct gactggcaat
     6361 agataaagta gccaaggatg tgctagtcgg atatctgtca cgaacgtcag gctatttaag
     6421 aaaaaagcag acaagctccc catgaatact ccgagaatcg ctgtgaccac cagccacttc
     6481 aacatgtaca tcaaaatacg cactggactc ggtaattgtt tttccataaa aaattcctcc
     6541 tcaaataaaa aaacgcctac taaagaaagg ggctggcccc agactttagt aggcgtcatt
     6601 agctatctta cgttagcatt taaaggcgaa caccatcacc tattcacttc cattaaaaga
     6661 tagcatattt ttctgtaaaa aacaataaaa ttatcgttat ttctttacac gttcagcaaa
     6721 ttcatagaaa ggagccaatt tcgttaactg ttgattacat tgatcactat catgacaaat
     6781 ataattcccc tttttcgtat acgtgccatc agaaccggct ttcgttgtag aaagaaagag
     6841 cgagacattc gatgttttct gacagatcgc acagattccc ttattaatcg ttggtgataa
     6901 agtaccgctg atccctttta attttccatc ttcgtaataa accatgaatt ttttttgcgt
     6961 ccctggatca ttccaaccga tgtacgtatg tgcttgcaag tcgatttcat tccaagcggg
     7021 ttgtttgagt tttttcactt ttcggaataa tttctcgatt tgtggtttgc tcggctgcat
     7081 aaacggctgg acgatttgct tcagctcctc caatgctcgt tctgcttgcg tttttgtcag
     7141 ttttacatct aaaactgtcg ttaaaaacgt atcgatttct gatggttcgt aacctaatac
     7201 atcgcgaatc ttttcagctg ctagagcctg aaccgtttgt accgtttttg agtcattgac
     7261 agatgagtac gcatttaata actgctctac ttctcttttt ataaaaaaat attgatatgg
     7321 tttcattgtt tgttccatga ttttaaagtc ctttcttcta accaaagtgc atactttgta
     7381 gaaaaaagtg atcttctgca aagtatgtgt tacctgaaga tcccggactc ttttaaataa
     7441 gatacgtttt gcatcttatc atcaccccct ttaatgttca ttcttctatg tacatgattc
     7501 attcgataaa taaaagtaaa ccgtatcttg agcagatagt caactgcaaa cagaaaaagg
     7561 actgtgacaa aagtcgtgtc aaagtccctt tctacaaaca aacggtgttc cagaagtagc
     7621 ttcttcaaaa aataagccgg aatttcacaa aaatttgaga agcaattttc gtaaattcct
     7681 tcttatttct tgaagctgac ccacttctgt cacaacctca atatggtcta ttattggttt
     7741 tacctgttaa atcgaggatt cttttccgaa gtaacgttgc catctcttac gcatacttgc
     7801 tgacataggc tgagcggctt ctttgactgc gcagtatttg tctacgaatg ttacgatata
     7861 tccttctttg tacttaggcg gtgcaatcgt tgcgccccac atgtgcttca aaatgatatc
     7921 tcgctcaaga tccgacaaat cggtgatctt ttctgcattc tttacagcga ttcttggatg
     7981 gatatacgca tgtgtgcctt catcaaattt cgtcgtacgc caatcatagt agaacagatc
     8041 atgcaaaagt ccagcacgcg ctgttgcttt agcattgcca ccccattttt ttgcgagcag
     8101 ataactatag taagaaacac tgatggaatg ctccaaacga gtggaatgaa catgctgtgt
     8161 gtaattcgct aattttttca ctgcttctgt ttctaaaaga tcttctacat aagacatata
     8221 ttcttcgtct tcacgccatt tttctgtcat aattatcgat ctgccacctt tttgaatttc
     8281 attttcttct gaaattgatc taagtatacc accagtgttc ggattattct agtcgccttg
     8341 gataaaatag ccaaagctta atagttcctt aatcataaag aaaaatgata aagaacaatc
     8401 cctgccgcaa ccccaacatt caacgattcc gcatttcctt tcatcgggat atatacgttc
     8461 tgatcagtca aagacaagat ttcctgactt accccctgac cttcgttccc catgattatt
     8521 gcgaagttat ctgatttttc tactttgtct aaggcgtgtg cttcttcatt taattctgtg
     8581 ccatacactg taaacccttt gtctttcatt aaaggaatca cttctgtcaa atctttttgc
     8641 aacacaggca aatgataatt gcttccctgc atgctgcgca gcacctttgt actataaata
     8701 tctgccgttc ctttgcctaa taaaacacca gccatatcaa atgcatctgc cgtacggatc
     8761 atcgtcccta catttccagg atcttgtaca ttatctaata acagccatcc tcctgaaaaa
     8821 tctggcgtgc cttcagccgt taacgccatc actgctaaga ttccttgtgg tgtaggtaga
     8881 tccgatacag catccatgac ctcatccgaa actagaaaag tagaaatctc tttgtttgcg
     8941 gccagccagt ctccccattc tttcatgcct cgctgtgtca caaaaagttc ttttatttct
     9001 gcatcagacg acgcagcttc ttcaatcaga tggaaccctt ccaaaagata ttcttccttt
     9061 tcttcccgat atttcttttt atgtagtttt tttaattctt tgattcttgg attttttgct
     9121 gattggatct ctttcacatt cctcacctcc ccatgattat atcatggaat aggtgcttga
     9181 gcttgtttca tcctaaaaaa atcggtatca ttaatttata aaataggaaa gaaaggtgac
     9241 gaacatgcgc aaagttaaaa tgaacgtcca aggacgcgtc caaggcgtag gatttcgcta
     9301 catgaccaag atggtcgctg atcaactagg cgtgactgga acggtaaaaa acgaagatga
     9361 cggctcagtc acgatcgaag caatcggcaa tgacgacatc atccagaaat ttattgaaga
     9421 agtcaagaaa tctccgagcc caagtggacg agtgcagtat gtggatgtcc aagaagaccc
     9481 gatgattgag gaacgaaaga aatttgatgt ggtgggataa ggttgtgaaa aagctggtct
     9541 gcatcaagca ttaaggacag gttgtgaaaa atagctctcc atatttttca acaggctgga
     9601 cttaatgccg tagatgcaag cttttgaaca ccgtcaataa ggttgtgaga aagtcgctta
     9661 gttttgagta gtaaggagaa ttttgtgaaa atggctttcc acatttttgc ataaaatttg
     9721 acttaatacc gagaaactga cttttgaaca ccgtcgataa ggttgttttt gagacgcttt
     9781 gcctcaagta ttaaagatag gtagtaaaaa agctgatcta taacttgcaa taaatttgct
     9841 taacccatat tttccagaca gaacaaaaaa gtttttagtt ttttaaataa gaaagaaaca
     9901 cttggaatca ctcttttctt tccaagtatt tctttcttga ttttttgtct ttttttcaaa
     9961 atttttcgct acgtaaaaga acgaatattt atatgaaatt tctatgaagt tgattgaatt
    10021 ccatgcgctt ttttagtata gttaagtttg tgtaaaaatt atagaagagg aagtattcta
    10081 tgaataaatg gaaaaactgg ttattgggat ccggacttgt cagtatcctg ctctttttat
    10141 ctggctgtgt gcggatggat tcaaacggaa accctgacac ttcgggaatc gtttatcgag
    10201 tactcgtcca tcctatgggc caagcgatta catatctagt tgagaatttc aattggtctt
    10261 atggctgggc agttattgca atgactgtga tcgtccgtat catcatcttg ccactaggta
    10321 ttagtcaatc gaaaaaaaca atgatccaat ctgaaaaaat gcaagcgtta aaaccacaag
    10381 ttgaagcagc ccaacaaaaa ttgaaagctg caacaacacg tgaagaacaa atggcagcac
    10441 aagcagaaat gcagcaagta taccgcgaaa acggattgag tatgaccggc ggaatcggct
    10501 gtctgcctct attgatccaa atgccgatct tctctgcctt gtactttacg gcacgttata
    10561 cagaaggtat tcgggaatca tctttctacg gcatcgattt aggctcacca agtatggtac
    10621 ttgttgtcat tgcaggggtc gcctatctgc ttcaaggata catctcaacg atcggtattc
    10681 ctgaagaaca gaaaaagacg atgcgtacga tgctaatcgt ctctcctgct atgatcgtct
    10741 tcatgtcgat cagtgcgcca gccggtgtta cactttactg ggtagtcggt ggtgtgttca
    10801 gctgtctgca aacattcatc acaaacgtta tgatgaaacc acgtttgaaa gcacaagtgg
    10861 cagaagagtt gaaacaaaac ccgccaaaac aagtcgtcac tccaagaaaa gacgtgacag
    10921 aatcagcaaa gccagctaac aacaaacaag caacaaaaca aatcaaacaa ccaaaaaaca
    10981 gtacaaacaa caaaggccgc aacgcaggaa agcagcagaa gcggaaataa aaacatagga
    11041 caaaaaagat gagagctaag ccctcatctt ttttgttact cttcctcttc tgctattcca
    11101 ttaattaatt cgttgacaga gacaatgctt tcacatcctg cacgaacaaa ctcgggctct
    11161 gaaccaatca tttgtgagtt gataaattca atgaccatcg gaagattcat cccagcataa
    11221 agagtaatct gttgtccttc cattattagt ttagaaatga tatttgcagg tgtaccacct
    11281 aaaagatcgc atagtaccac aaaatcattt aacgaatctg tgacagtgag aaacttttgt
    11341 ttgtatgttt cagtgccatc ttctggtaat aaaggtaacg tgtagatatc ctcttgaggc
    11401 cccatgatca tttctgtact ttttttcaat tcctcacaaa attttccgtg actaatcaat
    11461 accaattgtc tactcatttt tcgctctccc ttatttcgaa atcacaccca atgcagataa
    11521 agcaacacac acaattaata cgataaaaat cgctcgagta ggagtcatgt ttttctttcc
    11581 taataaccaa taaacaccac cgacgatccc agcaggtaaa attttaggta agatcatatc
    11641 gatatagttt tgcatattca atgtcacatc gccaatcgtt ggtgcccatg aaaatttaac
    11701 attgatcatt gtcgcaacta atgcccctac catgaatacc cctaataaag tggctgcgtc
    11761 cgttaatgca gttaagcgag attgcatagt agtaactaag gaaattcctt ctttgtatgc
    11821 aaattcaagt tgcttccaac gaaaaaccat gacaacgatc tgagcaaaaa tccatataaa
    11881 cacacctatc ggatttccat cagttgccat attcgccgcc aaagccccaa aaatcgttgg
    11941 aatcaaagca gcaaatacgg aatctccaat cgctgcaaag gaacccatca atccagcttt
    12001 cagccccgat acggtttcct ttgaagcaat tccctctttt tcttccattg caagatcaat
    12061 cccagtaatg attgtattaa aaaaattaga agtattaaaa aattgcgtat gtgttttcat
    12121 catctctttt aattctggtg tattgtctcc atatattttt cttagttgtg gcaagatagt
    12181 atataaatag ccagaaccct gcattctctc atagttccaa cctaattgaa aagtgaacaa
    12241 acttcttcga ttgatttgct taaaatcttc ttttgttaat ttgtaattag atttcgtcat
    12301 cttcgatttc cccctcaaca gaatgtgatg gctcactagc taatggttga tggtctttgt
    12361 tttgatcatt cttataactg atcattgcta gtgctagacc aatcaatgct acagcaagca
    12421 tcggcagcga attgaaagtt gccggaagat ctttaacaac gcttgccaca cttgttccta
    12481 ataattgaac attagagaat aacgttgcta ataatgccgt gatcgtaaat cctaaaataa
    12541 gataagatag atgttttttt actggtaaat agcgcagaag aatggcaaac ccaacagctg
    12601 gtaaaacagc tcctgctact gaaagtccat cgcctagcca ttttagattc ccgtttaaaa
    12661 cagaaacaac attttcaact aatccaccac caaatgctaa agctaaaaat acaggaatca
    12721 tacgagagag cgaccaaggt aaagctccca ttaagaaatt gcgttcgatt gcttgataat
    12781 tcatgtcttc tacgtgacgg tcaatacgat gggcaaagta tgtgttagcg aatctagcta
    12841 aaatatccaa ttggatcatt aagcttgcta caggcactgc aatagcagca atcgcttgtt
    12901 ctggattcat tccaagagaa atcgagaagg cagttgctaa aatcgtccca gagttggcat
    12961 ctatttttga tgcgcctcca aacgtcccca ctcctaaaac tgtcaactgc atactgccac
    13021 caataatcaa acctgcgttt aaatcaccca ttactaaacc tgcatataac cctgcaaaca
    13081 ctggcgcact cattgaagaa tagatctgta attcatctaa aatttgataa cctgcatata
    13141 atgttaataa taagatctgc caccattgaa tatccatgga tcattcctcc tatttgatta
    13201 aaagattcat aaagtcttct gctttatcgt ttggtaccat ttgagcaatt aaatgtatac
    13261 caagctgatc taatttttta aatacttcga tatctgcatc taccacattg attgattttg
    13321 taatggaccg tgtttcattc gtttgtgaca tattccccac atttacttct ttgataggaa
    13381 ctcctttttc tactaaagat aagattcgat ctggcttttt tgctacgatc aatactcgtt
    13441 gagagtcata tttgccagct aaaatatttg ttgctgcttt ttcgattggc aaaatgctta
    13501 attttacccc tgctggagtc gctaacttca atccactttt ttcaattgtg tttgtggcta
    13561 cctcatcatc cacgaccatg attcgactaa tattcagttt cgttgtccaa agatttgcta
    13621 cttatccatg tatcaaacgt ccatcgattc ttacaccaat aattgccatc atgcattctc
    13681 cttatctatt ttttctattt attgttcaat taaaactgtg acatcatcaa atatcccttc
    13741 tgtttcaaag ataacgtttt cattttttct ttcttatatt tcatttttaa gctaagaaca
    13801 aattccttcc caactaaaga aaacgctttc ttttatattt aagttcattt gattttctta
    13861 acttgaatgt gtttcatcaa catcgtaact ttcatagggt cgatccagcc agttttctct
    13921 tgttgcgcgt ttgccatacc acaagccata gaaaatgcga gaatctcttc gattgattgc
    13981 ctttttgata acgcatgggc cgatcctgca agggtcgcgt cacctgaacc aacaggatta
    14041 acaactgata ttttaggaat aatgacttcg taaaaacact gagcaacttt tgccacagca
    14101 cctctctctc ctaaagaaac aacaacccat ggcactcctt caaatattgg atgagctaat
    14161 aaggactgaa ttttttctaa ttgaatcgaa gaacagatac gttggccaat cagttctcct
    14221 aattcttcca gattgggctt gataagtagt ggcttctcct taccttccaa tacttcaagc
    14281 aaaaatttac cagaagtatc tagtaatatg tctttccctt ttttatcagc taaagcaacc
    14341 aattgcttat agtaatcaag agggcatcct tggggtaaac tacctgagat cgtcataata
    14401 tctgatattt cgaggagctg ctcgaaatga tcggtaaatg tctgttgttc tttctttgtt
    14461 atgaaggggc ctcgttccaa aagctctgtt tgtctcgaac tatgcaggat agcaatagaa
    14521 tcccttgtct ctcctttaat tggaacaaac tgatgcatga tccctaactg atctagctgg
    14581 ttctttagat attcaccgtg ctggccacct attaaaccag tagctatcac ctcttctcct
    14641 aataaattta gaaccctcgc cacattcact ccttttcctc ccgctgtttt attgactgtt
    14701 tcaactcgat tgactttgtc gaggtttaat tcttcaattg tatacgcgat atcgatggaa
    14761 ggattcaagg taactgtcgt aatcatttca tcttcacctt tttgcttttt gttttattga
    14821 accaaggggt agcggtttca tttattactt tatttaaagt ctcgatgttt tctctcccat
    14881 ttgttcttaa ccaatgaata gctccgtctt ttccattttc gacgtatgct ttgaccccat
    14941 ctttccatgt cgcacgtcca cacaatacac cattgaaggt ggaacccgat tcatgagcaa
    15001 aaatcagtgt ttgttgaaat aattctgttg aaacaccccc actcaaaaaa atgaatggta
    15061 atggagtcgc ttcactttgc tccttaaaga aggcagcagc ctcttctttg ctgtgaataa
    15121 attcacctgt ggaatagcca ttcacataat tcatattgac tggtacttct actttcaaca
    15181 catcaacttt atattttgga tcagaaaatt ctttcatcat ctcaataact ttgtggggct
    15241 tgactttcgc atactctgga gaagaactat cgcttatttg tgcatcataa gaaactaact
    15301 ccaaatagaa gggacgattt tctccttcgc actcactacc taaacgttcg atgaacacat
    15361 gcttcaaatg attgatttcg ggtgcttcat caacatcgta gtaaagtaag aattttactg
    15421 catccgcttg ctgttctttc aatctgagag aagaccaatc cgctaataaa tcaggtagac
    15481 gaccaggcgt agttgcgtca tagccggttt tctcataagc taacaataac cccgtctctt
    15541 ttgctctgac ttttgctgct ggcaatccat attccggatc caataaaata gcagaagcat
    15601 agggtgtcag ctcagctgat actagttctt tgaattcact gatttggtct tcacttggct
    15661 cttttcccaa cgatttgatc atttttttta gagcaccacg ctgatcgatc gctaatgcac
    15721 taataatccc atcttttgtt gatagtctct ccattgccct atttttttct tcattcatct
    15781 gtcatttctc cttcggtttc atacttatga ataatgacgc ccttgactac tcgattgacc
    15841 gtgcctgttg gagatggtgt atctggttta ttagctactt tgattgaagt atagagtgca
    15901 atgatttgtg cagtcataat catcgctaat gcaagatagg catcgggcaa tttactcacc
    15961 gagtcttcca atagcacagt catacctgag aaatcaatag tacccggttg cgcaatcgca
    16021 atgacattgg cagtgacccg atcttctttt atttcctcta aaatatctaa gtcataattt
    16081 cgcgtatatg gttgattgct cacaaaacct acaacgagtg tctcgctatt taaaaaagac
    16141 tttggtccat gacgaaaccc catcgaagaa tcaaaaattg tagcaacttt acctgctgtt
    16201 aattctaaaa tttttaattg tgcttctctt gtcgcacctg ccaaacttcc tgaacccaga
    16261 taagcgatcc gttcaaaaaa atcaggtaat aacttttgaa tttcttgatc tcttgaaaga
    16321 agatcttgac cgattttaac taattgttca agatatcttt cttttttctc cattgctgtt
    16381 tgatcaaaga tccataaagc actcaataac atacacgtaa aacttcctgt catagcaaaa
    16441 cctgcatcgt ttgctctctc tggcatcaat aacagataat tctgtttatc atcctgtgcg
    16501 ttttttgcca acatcccctc tttagcacaa gtaatcgtca aatgaaacaa tgtttcaact
    16561 tgttcatttg caatttgcac agccgctaca ctttctggac tgttgccact gcgagcaaat
    16621 gaaactaata aagtcggttc atttggaaac aaaaattctt ttggtgaagc tacaatatct
    16681 gtcgtgccaa aactgtggaa gataaaaaac tgattgtctc catgttcatt tagatagggt
    16741 aagagcgtat ccccaatata ttgagacgtt cctgcacccg taaaaataac acgtattctt
    16801 cttccgtttg cttctttttt tatttgatca aaaaatagag ctaaattttc ttggctttgc
    16861 atcaatctat cgattgtttc tttccagaga tctggttgtt gttttatttc ttgtgtcgtg
    16921 attgctgcac ctatttgttt taatttgttc ttcgatagct caaacatgtt gttctccctc
    16981 tcaatcattt tcggatatgc cgaattttat aatgaaactg gtccgctctt gctacactat
    17041 ctgtatattc gataatctca tttttcacgt tataggttat cctcgtcaga tgtaagacgg
    17101 gagcgccatc agcgattcgt agcacaacag catctttttt ttgcacgata cttgcataaa
    17161 actcttcatc agctaattga acttgttgtt cataatcctg tgaaaacaca tcatacaacg
    17221 gtttactttc aagtaacggc aaatcaagtc ccataaatag tttcgccggt agataagaac
    17281 gctcaaccat caatggctct tcatctgcca aacgcaatct tttaagttta aatacagttt
    17341 cacctaaatg gatgttcaaa ttcttagcaa ttctcttatt agcttccatt tgtctaaatt
    17401 ctaaaatgac cgtcttaggg acgcgcccta tttcgcgcat ttcttctgta aagctatatg
    17461 ctccagctaa gttcgatgac ttatcagaca aatcagagac ataggtacct tttccatgtc
    17521 ttcggtaaat ataaccttga ttttccaatt cctgtaaagc cagacgtacg gtggtacgac
    17581 tcaccccata gtgttgacaa agttctcttt cagataacag cttatcatgc ggagtcatct
    17641 gtgtttcgat acgatccttc aataaatcaa ctagttgata atacaatgat tttcctttat
    17701 ttgagctctc cataaaaatc acctcttctt tactggttat aaccacaaca caagaatagt
    17761 gaattattaa tcatttgtca aaggtttttt agaccaaaaa agctattata acagctcttt
    17821 aaagctttat atcttgaata gacaaagata cctcaaaaat accagttacc aataaaagaa
    17881 cagaaaaaaa gacgaaccac ataagcgatc cgtcaaaatg gtctaactta tcgagcagcg
    17941 tgatccacat taatccctat gatattccta caactatcgt tttattactt ccaatatcat
    18001 ttacaacaac taaaaatagc ttattaatga caaatgttat tattagtttt agataacttt
    18061 tgcagtttta tactttttt
//
LOCUS       DARDHA010000044        17727 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000044 DARDHA010000000
VERSION     DARDHA010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.044"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            66..1751
                     /locus_tag="SDA10_001218"
     CDS             66..1751
                     /locus_tag="SDA10_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HEG4240141.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            1765..3048
                     /locus_tag="SDA10_001219"
     CDS             1765..3048
                     /locus_tag="SDA10_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEG4240142.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            3118..3987
                     /locus_tag="SDA10_001220"
     CDS             3118..3987
                     /locus_tag="SDA10_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4240143.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            3984..4820
                     /locus_tag="SDA10_001221"
     CDS             3984..4820
                     /locus_tag="SDA10_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4240144.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            4846..5913
                     /locus_tag="SDA10_001222"
     CDS             4846..5913
                     /locus_tag="SDA10_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEG4240145.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            5910..6737
                     /locus_tag="SDA10_001223"
     CDS             5910..6737
                     /locus_tag="SDA10_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG4240146.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            6782..7807
                     /locus_tag="SDA10_001224"
     CDS             6782..7807
                     /locus_tag="SDA10_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG4240147.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            7804..9987
                     /locus_tag="SDA10_001225"
     CDS             7804..9987
                     /locus_tag="SDA10_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEG4240148.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            9987..11117
                     /locus_tag="SDA10_001226"
     CDS             9987..11117
                     /locus_tag="SDA10_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG4240149.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            11114..11791
                     /gene="pgmB"
                     /locus_tag="SDA10_001227"
     CDS             11114..11791
                     /gene="pgmB"
                     /locus_tag="SDA10_001227"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEG4240150.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            11875..12402
                     /gene="msrA"
                     /locus_tag="SDA10_001228"
     CDS             11875..12402
                     /gene="msrA"
                     /locus_tag="SDA10_001228"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG4240151.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(12603..13289)
                     /locus_tag="SDA10_001229"
     CDS             complement(12603..13289)
                     /locus_tag="SDA10_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEG4240152.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            13369..14907
                     /locus_tag="SDA10_001230"
     CDS             13369..14907
                     /locus_tag="SDA10_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEG4240153.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            15027..15494
                     /locus_tag="SDA10_001231"
     CDS             15027..15494
                     /locus_tag="SDA10_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEG4240154.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            16013..16456
                     /gene="rplM"
                     /locus_tag="SDA10_001232"
     CDS             16013..16456
                     /gene="rplM"
                     /locus_tag="SDA10_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEG4240155.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            16472..16864
                     /gene="rpsI"
                     /locus_tag="SDA10_001233"
     CDS             16472..16864
                     /gene="rpsI"
                     /locus_tag="SDA10_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEG4240156.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(16952..>17727)
                     /locus_tag="SDA10_001234"
     CDS             complement(16952..>17727)
                     /locus_tag="SDA10_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4240157.1"
                     /translation="VRSMFDIAEILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQ
                     ALSSKELTNWIEAVNDDFNNHLLTFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQT
                     VRLKHTLDKYQRKKFTKGRKDTVIQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYL
                     FTYTKPSGEVNCPVHADYLNYRINSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQ
                     ISNALTHSDISTTKIYVNTPDIVDKAVFEAFQRGLNKCD"
BASE COUNT         5765 a         3250 c         3809 g         4903 t
ORIGIN      
        1 agtaagcgtt ttccataata tgatcaaatc gttgggaacg ctcccaaaaa agaaagggga
       61 atccgatgta tacaaaactt ttacaagcga tttatcgcga ctcagatttg tctgagaaaa
      121 ccgcctctat gattgaacaa aaagtcagtg tttatcagca gaagaaaggc caggcaggca
      181 aactgcattg ggacgaagaa gatattttct tgattactta tggggatcaa ttttttgaag
      241 agcagcaacc aactttgcgt acatttaaga aattttatca aaagtatttg gcagatacat
      301 ttagagttgt acatttcttg ccatttttcc catatacatc tgatgatggt ttctccgttg
      361 taaattatca ggcaatcaac gaccgtttgg gcacatggga agatatagaa gaaatgagaa
      421 gcgaaacacg gttaatgttc gactttgtct gcaatcatat gtctgccgaa agtacttggt
      481 ttcaggaata tttaaacttg aatgatacgt acaaagattt ttttattgat ttgccactgg
      541 atacagatac aagcagcgtg actagaccaa ggaccagccc gttacttaca acgttcattg
      601 acaaaaacgg gaaagaaaaa cacgtatgga cgacttttag cgaagatcaa gtagacttga
      661 attttaaaaa tccagaagtt ttattgaaaa tgatcgatat tcttttgttt tacttagaaa
      721 aaggcgcttc atttattcgc ttggacgctg tgggattttt atggaaagaa ctaggaactt
      781 cttgtataca cctgccagaa acgcataaaa tcatccaatt attccgtgca atcgctgatg
      841 aagcagctcc aggaacggta ttgatcactg agacaaatgt gccacacaaa gataatatca
      901 gttattttgg gaatggcgag aatgaagcac agatggtcta tcagttttcg ctaccacctt
      961 tgacccttca agcgattcgt tcgggtaata caagtaagtt gaaagaatgg ttaaaaacca
     1021 tcgatttcgc atcgaaaaaa acaacttttt ttaatttttt agcctcccat gacggtatcg
     1081 gattgaatcc ggtacgaggt attctgccgg aaaaaacgat ccttgaatta gtggaacagc
     1141 tgaagaaaga gggtgcccga gtaaactaca agaaaaaccc agatggaacg accagtccat
     1201 atgagataaa cgctacgtat ctagatgcgt taagttctcc aacagatacg acggaatgga
     1261 gaagcaaacg ctttttagct gctcattcat ttttggtaac gttaccaggc gtgccggcaa
     1321 tttatattca aagtgtgctg ggaagtcgaa atgattacaa aggcgtagaa aaaacaggag
     1381 agaatcgaaa aatcaatcgg caaaaatttt tgttagcaga tattgagaaa gagttgcaga
     1441 cagatccatt aagaacagca gtatatggtg aattgaccca gatgctatct attcgaaaaa
     1501 aagaaaaagc cttccatccg aatagtttga tttacttact ggactcagca gattctttta
     1561 tcagttttat tcgcgaagcg aaggaagaga aagtcttagt tatccataat ttttccaatc
     1621 aatcagcagg ttatctttta ccaaaaggag attacctaga cctgttttct ggacaaatta
     1681 tccattgcag tcaagaacga aagttgatat tccagccgta tgaatatatg tggctgaaaa
     1741 taataaaata agaggtggaa aaaaatgaaa aaaattattg ttactacctg tctgctagct
     1801 actggatttc ttcttactgc ttgtggtgaa agtcaaagct ctaacgaatc acaacagaca
     1861 acgattcaat tcatgcactc ttctgtcgaa caggaacgat taagtgtgat caatgaactg
     1921 gttgctgatt ttgaaaaaga aaatccagac atcaaaatca agcaagtgcc ggtagaagaa
     1981 tcagcgttca atacaaaaat cgttacgtta gctcgttccg gtaaattacc agaagtaatg
     2041 gaagtcagtc aggattttgc taaagtgatg gataaagatg aactgattga tcagacggca
     2101 gtccaaaacg ttatcgagga agctggagaa gataattatt atgacggagc caaaaacctt
     2161 gttcgttcag aagatggaaa aagctatatt gctgcaccaa ttagcggctg ggttcaaggt
     2221 atttggtatg acaaagaaaa attatcagaa gctggttttt cagaaccaga aaattgggat
     2281 gatattttaa aaattgctca acatttcaca gataaagaga ataaacaata tggaattgct
     2341 atgcctactg ctgaaggaac aatgagtgag caagcgttct ctcaatttgc tttgtcaaat
     2401 ggagcaaatg ttttggatga tgaaggcgag gtaacgatcg atacggaaaa gatgagagaa
     2461 gcattgtcgt tttatcaaaa cttatcccaa tacacgatgc caggctctaa tgatgtgaca
     2521 gaaatcaaag atgctttcat gaatggaaca gcaccaatgg caatctattc aacttacatt
     2581 ttgccttctg tttatgaagc ggggaactca gaaaatatcg gttttgctat cccaacacaa
     2641 aaagatcaag ctgtctatgg cacagtatct ggattgacga tttcttctgg tctagacgag
     2701 aaacaaaaag aggctgctga agcattcaca gctttcttat ctcaaccgaa aaacatggaa
     2761 aagtgggtct tgatgtctcc tggtggtgct caaccagtga ataaacaagt agtcgaactg
     2821 gatggttaca aagaaaatga agtcatcaag tcttttggcg agctgccatc tgagattgct
     2881 agtgcttttg atgaagtaca agtctttgga ctagtcgggg aaaagaattt cacgaaaatg
     2941 ggagatatca caagctcagg tgtgatcgga aaagcagtga accaagtaac tgtcggaaac
     3001 gaagatgtcg atcaagcttt agctgacgca caaaaaaata cgaaataaca agaagttctg
     3061 ccaatggtgt tcgaaaaaag tagatagcta ttggcagatt atcaggggga ggtttcaatg
     3121 aatcgtccaa ggaaacattc tgacttaaaa ttagccatgg gacttttagc accaagcatg
     3181 cttttattag ctgttttagt gctgattcca atgctttcca atattcagat cagttttttc
     3241 gatatgccgc tgaatcctaa ctcagacgcc gtttttatcg gattaagcaa ttatcttgaa
     3301 attttaagtg atagtgaatt ttatcgttct ttaggtttaa cagttgctta cacattctta
     3361 gttgtagcag gcagtacagc tttaggatta gccgtagcta ttttgcttaa ccgcaagttc
     3421 agatttagga aaacggtgcg ttcactgatt ttattaagtt atgttacgcc atctatttct
     3481 ttgatttttg cttggaaata tatgttcaat aatagttatg gaatcgtcaa tgatctgtta
     3541 gtagataggt tgcagctttt caacgaagca ccgctgtggt tcgaccagcc agttgctagc
     3601 ttctttatgg tcgttttgtt tgctatctgg agatactatc cgtatgcgtt tatttcattt
     3661 ttggctattt tgcaaacagt cgacaaaacg ctttatgaag cagctgaaat ggacggtgca
     3721 aatgcttggc agaagttcaa gatcgtgaca ctgccggcaa tcaaaccagt actagcaact
     3781 gtgattgttt tacgatctat ctggatgttc tatatgttta cagatgtcta tctactgacg
     3841 aataaagtca atatcttagg cgtttatttg tatcagacag cctttgcgtt caatgatctt
     3901 ggaaaagcag cagctatatc gattctattg ttcatcatta ttgcagcggt cgtcttgctc
     3961 tcacgaaaga aggtggactt gaaatgaaaa ataaaaagag caggaaaatc ttattttacg
     4021 cgacgatttt gttgctatta gctgtcaatt tgtttccatt tttgatcatg ctgatgacat
     4081 cattcaagga ttcacaagaa gcaattgcaa cgacacaaac tatttttccg aaagaatgga
     4141 cttttgaaca ttatcaggat attttcaatg ctgagatttt tccattcatc atgtatttca
     4201 aaaatagttt agtgatctcc ttgatagcct ccgtaactgc agtagtgatt gggatcagcg
     4261 gggcatacgc tttgtcaaaa ctacgcttca aagctcgctc gaaaatcaat gctagctttt
     4321 acaccgttta tatgttttcc ggtattttga tcgttgttcc attatttaaa atcatttccg
     4381 gtctaggttt gtatgatacc aatacagcac tgattatcac gatggtcgtt caaacacttc
     4441 ctactgcgat attcatgcta aaaagttatt ttgacacgat tccgactgat attgaagaag
     4501 cagcaatgat cgatggtctt agccggatcc agatcattat tcggatcatt ataccattgg
     4561 ctatttctgg aatcatttct gtcttcgttt attgctttat ggtttcgtgg aatgattatt
     4621 tatttgcttc gattttcctg tcgacctccg aaaaatttac tttaccaatc ggtttgaaca
     4681 cattattcag tacaccagac tacatttggg gacgaatgat ggccgcttca ttggtgaccg
     4741 ctttaccagt catcattatg tatgcgttat ctgaacgatt tattaaaggt gatttagtcg
     4801 aaggcggcgt aaaaggataa aaagaaaaga aagaggaaag aaaagatgaa aaaattagtt
     4861 gcattagaac caagaatagc cggattcaaa gattacgaag accgtgagat cgaaaagaac
     4921 gaagtacgta tccgagtaat tttcgctact ccaaaaccag gaacagaaat tgtcgacttt
     4981 agaggattaa gtccatttat cgatgaagaa ttttcagaag aatggcgcct attcttgcca
     5041 agagaagaag gaagccaacg cggtgtggta tttggcgaat ttcaattagg aaatatgatt
     5101 gtcggtacga tcgaagaaac gggcagtttg gtgacagagt atcaaaaagg agagttagtc
     5161 tgcacttatg gacccattcg tgaaacgctg attacaaacg gtgttgataa ttataaacta
     5221 cgtaaacttg atcggttgga tcaatggaaa aatgcagtat gttacgatcc tgctcaattc
     5281 gctttaggtg gtatccggga agcacatgtt cgagcaggtg atgctgtagc aatcttcggt
     5341 ttaggcgcta tcgggcaaat tgcgattcaa ttagctaaaa aagcaggtgc tactaccatc
     5401 gttgggatcg atcccatagc gcagcgtaga gaattagcag aaaaacacgg tgccgattac
     5461 acgctagatc ctatgaaagc agatgtcggt tttcgtctaa aggaattgac agataaacgt
     5521 ggagtagatt cagttattga aacgagtggc agtgctgccg cattacaagc ttctttaata
     5581 gggatcggct atggcgggat catttcttat gttgcctttg ctaaaccttt tccagctggg
     5641 tttaatcttg gtcgcgaagc acatttcaat aatgcaaaaa tcgtctttgc tcgagcatct
     5701 agtgaaccaa acccagatta cccaagatgg aaccgtaaac gaatcgagga cacatgttgg
     5761 gaattattga tgaacggtta cttggattgt gaagaaatca ttgatccagt tgtgccattt
     5821 gcagaaagcg cagaagctta catgcgttat gtagaccagc acccagaaga aagtatcaaa
     5881 atgggcgttc aagtccgagg aggagaacaa tgaaattcgg tacgcaagat caagcatttt
     5941 tcccaagaga cattttagaa aaatttagat ttatcaaaga ctgtggattt gaagaatacg
     6001 aaatcgacgg taagttgtta atagaaaata tcgacacagt caaacaggca atcaaagaaa
     6061 cagggatccc agtagtgaca gcttgtaacg gctacgatgg gtggatcggt gactttatcg
     6121 aagaacggcg tcttaatggt gtacgtcaaa ttgctgagat tttgcgtgca ctaagcgagg
     6181 ttggaggcaa aggaatcgtt atcccagcag cttggggaat gttcacttat cgattaccgc
     6241 cgatggtttc accccgaagc aaagaaggag actataaagc agtcagcgaa tcacttcgtt
     6301 atttagataa agtagccgaa gaaacaggta cggttattta tttggagcct ttgaaccgat
     6361 accaagacca tatgatcaat ttgttagcgg atgcgcgtca ttacattgac tctaatcaat
     6421 tgaaacatgt gcagatcatc gctgattttt atcatatgaa tattgaagaa gatgatattt
     6481 caaaagcatt gactacgcaa aaagagtatg tgggtcatat ccatttagca gataatcatc
     6541 gctaccaacc aggatctggc gcaattgatt ttactcgcca ttttcaaacg ctacttcaaa
     6601 atgagtatga aggttatatg gtatatgaat gtcgtttgcg aggagaaaat gaagtgaaaa
     6661 tttatcagga atcattgcaa tacttgaaaa actgtctgga aacagcgcgt catttaaatg
     6721 atttagagaa agaatagaga aagaataaca ataagtaaat aagcaaagga gagagaaata
     6781 aatgaagaaa gtagcaatca taggcgctgg acaagtagca gaaaaagtac atgctgctta
     6841 ttatcaaaaa agatcagatg tcttagaatt ggccgctgtg gttgatccag tcagccagcg
     6901 agcagaagac ttttgccgac gtacaggatt ttctcatgcg tatacatcag ttacacaaat
     6961 gctgaatgag atttctcccg atatcgtgag tgtctgtaca cccaatcgct ttcacttcga
     7021 acatgtattg gctgccctcg aagctaatgc ttcagtgatg tgcgagaaac caccagctat
     7081 gacagctgat caagcaagaa agatggcaga gacagccgat aaaaacgggc agattttggc
     7141 ttatggattc cagcatcgtt tttctgacga agcaacaatg atcaagaatc atcttgatgt
     7201 tctcggggat atttattacg tagaagcgaa tgcgcttcgt cggtctggtg ttccaggctg
     7261 gggaacgttt atcgacaaag aacttcaagg aggcgggcct ttgatcgatt atggcattca
     7321 catgcttgat acagcgatgt atattactgg atttcctaaa gtaaaaagcg ttcgggcgta
     7381 cagcttccaa aaaattggta cgaaaaaaaa ttcagggaaa ttcgggaaat gggatccaag
     7441 caaattcaca gtagaagatg caatttttgg cacactggaa ttagaaaacg gcaagatcat
     7501 ccaactgaat acttcgtttg ctttgaatat cagagaagac aaactgaatg tggctttttg
     7561 tggcgatcag gcaggtgcta atctgtatcc aggcgaacta ttcgaagatg tagatggaaa
     7621 gctggtttcc ttacaaaaga ccacgccagt taatgaccca catgaactaa gtatggctca
     7681 ttttgtgaat cgggcgctcg gtgaccagac tcagatgatt gcagatggct ggcaaggata
     7741 tgtcatccag aatctggtgg aaaatttata tcgttcggca gaaacaggag aagttattac
     7801 cttatgaaag tagaagaaaa acagtttgat ttagagtata tcaataagta tgcgagtcag
     7861 atgaccattg gaaatggatt ttttggtatc agaggaagcc aggaggaaga ctatcggaat
     7921 caagtaaggg gaatgtttgc agctggtgta tataaccgac ctttaggagc agaatcagcc
     7981 gagttagtga atctcccaga tgtcatcaga tgggaaataa aaatcgacgg tgaacatttt
     8041 tctcttgaga cgatgaaagt tcttgcatac gagcgggcac tagatttaga aatgggagag
     8101 cttaaacgct atgcgctcat tgaaactgct caaggaaaaa aagtggaagt cattacttgt
     8161 cgtgttgctg atcaaaataa tctccatcag gcagccctga aagtggtcat caagccgttg
     8221 accgaagact gccaagtaga tgtgaaaacg ggtatcgatg gacagcaaac caattttggt
     8281 acacagcagt tggttgaaaa agaactgcga gtatttggtg acgatttgat cagtgcatcc
     8341 taccagacaa cagaaagtaa aattgatatt gggattgcag ctaaattcaa taaagcaggt
     8401 gtattccaag caaaaaatcg ccggatcacc gcttcctttt cagaaaagat cagaaaaaat
     8461 acgtccttta cgctggagaa gatcatcgtg ctctctacct ctcttcagca aacagacccg
     8521 tttaagaaaa gtattgccca agcccagaaa agcgttagtt atacagaaat tttagagcag
     8581 tcgatgtctt attggacaga tttttggcat cagcatcgga taacgatcca aggcgattct
     8641 tctttcgatc agttagcgat ggacttcgct gcttaccatt tacagattat gactccccgc
     8701 aatgatccaa gtgttagtgt aggggcgaag ggactaactg gcgaaggtta taaagggcat
     8761 gttttctggg atacggaaat ttttatcctg cctttctttt tatatcagtt tcctgaagta
     8821 gcaaagaatt tgctgctgta ccgttatgat cgattagaag gcgcacggca aaaagcaaga
     8881 gattatcact atcaaggtgc acttttccca tgggaaagcg cacacactgg tcaggaagag
     8941 acacccaaat ttgctgcatt gaatatcaaa accggaacaa gacaagaagt tgcttcagct
     9001 gccgcagaac atcatatcag tgcagacctt gcttatgccg ttcaagagta tgtccaagca
     9061 acagaggatc aatcgttttt agaaacaaag ggaaaaacgc tcattcatga aacagcagct
     9121 ttttggctga gcagagctgt aaaagtcgat gatcatttag agatacacag agtcattggt
     9181 ccagatgaat acacagaata tatcgacaat aatgcatata cgaactatct ggcgcattat
     9241 aatgtggcct tagctgttaa atatcaagct ggcgatccgc tttttttgaa agaatgtcaa
     9301 aattttttag ataagttata tttgccagtg cctaacaaag aacagctgat tccgcaagat
     9361 gacacgtatc tcagtaagcc agttattgat ttgaccactt acaaagaacg tcctggcagc
     9421 cagtctattt tacttgatta tgcccgtagc gaagtaaatg aactccaagt cagcaagcaa
     9481 gcagatcttg tcatgctgtt atacttatta cctgagtatt tttctcaacc aatcgtttct
     9541 aagaatttgc attactatga aaacaaaaca atccacgact catcgttgag taaagcgatt
     9601 cacgccatag aggcttgccg tatccaagaa atggaatggg cgtatcattt attccaagaa
     9661 gcgtgcctga ttgatttagg accagatccg cattcatcag atgacggaat tcatgctgct
     9721 tcattaggag cactatggct agcagttata tttggttttt caggtattac agtaacgaat
     9781 ggaactcttt ctatttctcc aagacttcct tcagcatggg aaaaaatttc ttttcctttt
     9841 atttggaaaa atcacaggtt gctatttgtt ttgacaaaaa aacaaatagt aataaaaaaa
     9901 gagacgaaag aaacattacc tatttttgtc aatggagacc aataccagct ggaaaaagaa
     9961 ctgatcttgg aacggagggg aaaataatgc gtattctgat tgcgccagat tctttcaaag
    10021 aaagtctgac agccttagag gcagcacaag ccattcaatc aggttttcaa gaagtttttg
    10081 ttcaggctga atatgatctg ctacctatag gcgatggagg agaaggaacg gtttcatcat
    10141 tagcagacag tttaaggttg aagcggcaaa cagcagtcgt tactaatgca tttggcaaaa
    10201 acaaagaaat ccagtacgct tcagacggtc aaactgcgat ttttgaaatg gcagaaatcg
    10261 tcggattgaa agatgtccca ctaaaccaac aacagccact taggttatcc acgcgaggag
    10321 taggagaact gatacgacaa ttgttgcaac aaggtgttcg acacatcatc atcggcgttg
    10381 gcggaagtag taccaacgat ggcggcatcg gcatggcaga aggtctaggt tatgcttttt
    10441 acgatcaaga taatcaaaaa gtagaagcac tgggagaaaa tctaggtaaa attgctacag
    10501 ttgataaaac gaacgtgcct attgagctga ttaaagccaa aatcactgtc attactgatg
    10561 ttactaatct tttatgcgga aaagaaggcg cttcttttgt tttcggacca caaaaaggct
    10621 tgaaagaaaa tgaattagca gtggctgacc aagccatgca tgatttttat atgagatttg
    10681 cccctcaagt aatcgaggca tcgggcagta gtgctggtgg tggaatgggt gcaggactgt
    10741 tagcatttgc ttcagggaaa atagtctctg gcatcgacta cgtattggat ttactggagt
    10801 tcgataaacg ggtacagcaa gcagacctcg taatcgtagg cgaaggacgt ctagatggac
    10861 aaagtctaaa aggaaaagcg ccagttggga ttgctagaag agtaccagag ggcgttcctg
    10921 tgatagctat ttgcggaagt gttggtaaag gaagcgaaaa agcagcagat gttggcattt
    10981 cggccgtttt tccgatcctt cctgcagctg tgcctttaca agaagcttta cagcaagcca
    11041 ctgataatct tcatcgcaca gctaaaaaca tcgcatcttt acttcaagtc gttcattttc
    11101 aaggaggtaa tcgatgaaaa tcaaagcagc actattcgat ttagatggcg ttcttgtaga
    11161 cacagctcgt tatcattacg aagcgtggct ggttttagct aaccagctgt ccattccatt
    11221 cactgagaaa gaaaacgagc aactgaaagg aatatccaga accgagtcac tagagcgatt
    11281 gctgtcattt ggaaaaatgg aacagaaatt ctcagaaaaa gagaaatcag cctttgctga
    11341 gcaaaaaaac aatctatatc ttcaagcgat tcaaaaaatg gatgaaacat ccgttttgcc
    11401 tggtgcaata gctgttcttg agtacttgaa aaaaacaaac atcaagatcg gattgggatc
    11461 agcaagcaaa aatgcacgcc tcattttaga aaaaacgaat ttgacaagtt attttgatgt
    11521 ccttattgac ggcacgcaag tatcaaaagc aaaacctgat ccagaagttt ttctcaaagg
    11581 agcacagcaa ttgaatgtac cgccaaatgc atgcttggtc attgaagatt cagaagctgg
    11641 ttgtcaagca gctttggctg gtaacatgca tgtattggga ataggtgaaa acataaatct
    11701 tccttctgct gaatatgtga ttcctgattt aaccgttttt gatcaagtac gttcgttttg
    11761 gcatttatca gaagccgtat cacgtatata atagtttccg ttttttctgt cacaagcaaa
    11821 acgttatcct ccttttggaa atcaaggtat gatgagaaaa aagggggatt ttatatgaca
    11881 aaaaaagcag ttttcgcagg cggatgcttt tggtgtatgg tcaaaccatt tgaagagcag
    11941 ccagggatca tttctgtgac atccggttat actggcgggc atgtaccaaa tccaacttat
    12001 gaacaagtaa caacaggcac aacagggcat acagaagcag tagagatcga atatgatcca
    12061 gaagttattt cttatgatca gttggtggaa atctattggc agcaaaccga tccgactgac
    12121 gcattaggac agttcgctga tcgcggtgat tcttatcgtc cagtgatcta ctataatgat
    12181 gaagaagaac gaaatattgc agagcgttca aaacaagcat tgcaagacag cgggcgattc
    12241 gataagccaa tcgtgacaaa aatcgaacca agaagcgaat tttatcctgc agaagattac
    12301 catcaggatt attacaagaa aaacaagctc cattacaatc tttatcgaga aggttctgga
    12361 cgtgcaggtt tccttcgtcg acattggggc gtcaagaaat aagaagccga aaaagctact
    12421 tatggaacac cgtatatcag aggttgtaaa accagcattt tgagctaata ctgagggtca
    12481 gctgattgaa caccatttag tggagaggtt gtgaacctgt cgcctagctc caagtcacaa
    12541 ggatttggta agagaacact atatatcaga ggttgtgaca agtcgtttgt cacaaccttt
    12601 tttcaatacc taagatgaag gacaatcagc aacgcactgc tcaaaaaagg aatgaaaggc
    12661 agcttttcca actttttttt atgacagata aagtaaacag aaaaaacgga tattccaaac
    12721 agactagcaa gaaacaagat ctgaagcaac tcttctagag acaagccacc actccaagaa
    12781 agcatcaaca gagtatcgcc agctccgaga taggattgac catagcgaag gcacacaccg
    12841 cctacgataa acgttaacag cacactttgc cattggaaga cgccggcctg caaccaaaaa
    12901 atccataaaa ttgcccaaga acagaatagc gtgtgagaat ggaccatgaa gtaaaataaa
    12961 tccatcaacg aaaatagcaa agccgaaagc atccatatga acgtcgaaaa atttcttgcg
    13021 gaagaaaaag taaagaacca ggcaaaaaga ccaccacata aggcttctgc caacaaatag
    13081 tagaagggca accgacacct gcaatagcgg cagcgaaatc gttggacgat gatcgagaga
    13141 atcggaatca attcatacca agaaagcgaa cgacagcatt tgacacaatg agatctagga
    13201 taaataatag agtgacctaa cggaatccgc tgggcggtta aacataaaaa tgatccgata
    13261 cagcaaccaa tgataaaata aagtaacacg ccatctcccc cttttcaaaa ccaagatacg
    13321 caagcttaaa ctaaattttt gtacatacat aaaatttgag gtgaaaaaat gaacgtttca
    13381 atcggattag atatcggaac cacacaaacc aaagcagttg ctttcacaga tgaagcccaa
    13441 gaagcagcat ccgcttattt tcgctaccct ttgatccaag aaacacaagg gatggcagaa
    13501 caagatcagg aattgatctt tcaaggtgtc tgcaccgtta taaaagaagt aattaaacag
    13561 ttgccttcag acacaatcat tcaatttgtc tccttctcct ctgcgatgca tggcctgata
    13621 ttgttggatg aagagaaaac accgttatct cgaatgatca cttgggcaga taaccgagca
    13681 gcggacgaag cagaaaaact gaaatatacc gtgcttggca aagaatttta tcggcagact
    13741 gggacgccga ttcatccaat gacaccattg aacaaaatca agtggctgca aaacagccaa
    13801 cctgaattat tcaaaaaaac tgcttatttt gtcggtatca aagactatat cttttataga
    13861 ttgtttggcg aactgatcag cgactacgga acagcttcag gaactggata ttttgatatc
    13921 catgaattcc aatgggcaga agaagtgctt gactacttga acattcgaat agagcaattg
    13981 ccgcaagtct ttccttccac ctatcaagta acaggtctac catcaaaaac agcagcagaa
    14041 ctaggtttgc caaatgatat atcatttgtt ttgggagggg cagacgggcc gctttctaat
    14101 ctagggttag gtgcttttgg tgatacgatc gccgctttga ccgtaggaac gagcggagca
    14161 cttcgtttta ttactactca gcctcagttc catccagaaa tggagacgtt ttgttatgtt
    14221 ctggatcaga aacattgggt agtgggagga gcgactagca atggtgctgg catctttgat
    14281 tgggcagggc atacattttt acaggaagtc gttcaatctg ctaaagaaaa aggagaaaat
    14341 ccatatgatg cactgctttc ggctatcgct tttgtgccag cgggtgccaa tggtctgatt
    14401 tttcatccct atttacttgg tgagcgagca ccgctttggg acgcagatgc gtttggcagt
    14461 tttatcggtt tacagcggca acatacagaa aaagaaatga tgaaggcgat tctagaaggg
    14521 atctgtctca acctttatcg gattttaaaa ggagtttgcc aggaaaatca gctcacagag
    14581 atccgtgcta caggcgggtt tgctcaatct ccgatttttc gtcagatgat ggctgatgta
    14641 ctgggttacc cgcttgtttt ccctactgtc acagaagcat ccgcattagg agctgtgatt
    14701 ttaggctgga gaagcatcgg aaagatccaa agtatcagtg aagcacagga attgatagaa
    14761 ttgaaagagc aagttggagt acaagaaact gatcataaaa aatatcagaa aatctatcca
    14821 ttatttgttt cgacacaaaa gcatttagca cagacttatc agcaatttgc tcaactgcga
    14881 gaagaattag ctgattctga aaaatgattg gctttacgga taaaaaagcg aactgaaaat
    14941 cgttctcagg taaatgattt tgttgtactt ttcattgaaa gtctactatt attttaagtg
    15001 taagaaaatg ttaggaggca agaaagatga aattccaaca aaccaaagaa gtattaaatc
    15061 aattagttgc agatctaagc caaatgtctg ttgttgttca ccaaacacat tggtacatga
    15121 gaggaccagg tttccttaca ttgcacccaa tgatggatga gttcatggat gatttgaacg
    15181 ctcagttaga tgaagtatcc gaacgcttga tcacattaga cggttcacct tattctacat
    15241 tgcgtgaatt tgcagataac acaaaaatcc ctgatgaaat tggccgctgg gatcgtacta
    15301 ttccagaacg tttggaaaca cttgttgcag gctatcgtta tctagcagat ctttaccaaa
    15361 aaggaatcga agtttctgga gaagaaggcg acgatccaac acaagatatc tttatcgaat
    15421 tcaaaacagc cacagaaaaa cgtatctgga tgatccaagc tcatttgggc aaagcgccag
    15481 agatcgacgc gtaaaaactg ttgttgaact tgaccgaaaa agaggttgtg aaatcagcgt
    15541 tttgacctga gtattagaga aacaaacgtc aaaatagttc ctcatatttt gccgtttttt
    15601 gagctaatac cgagggtcag ctgattgaac accgtttacc aagaggttgt aagcctgccg
    15661 ttcagctccg agtcacaaga aacaatttta aaaaacagct tcttatgttt tatggatatt
    15721 gtgacttgtg accgaggagt tggcaggaga acaccgttta ttcaaaataa ggggttgtga
    15781 caagactttt gtcacagcct ctttttgttt ttcattttga caggaaacga cccttttata
    15841 taaaagaaag aaaagaagta aagtttcata taaatttttc aatcaagcag aaaaacgttg
    15901 acattgacta gattagcggg taatatgtta aatggtattg tttgccccac aaagagcccc
    15961 ggaaactcga agtgtatgtc aaacatcaga acttaaattg gaggaaaata acgtgcgtac
    16021 aacatatatg gccaaaccag gcgaagtaga acgtaaatgg tatgtagtag acgcaactga
    16081 tgttccattg ggacgtcttt caacagttgt tgcatctgta ctacgtggta aaaataaacc
    16141 aactttcaca cctcatttgg atactggcga tttcgtcatc gtcatcaatg cagacaaagt
    16201 gaaattaaca ggtaaaaaag cgactgacaa aatttattac cgtcacagca actacccagg
    16261 aggattgaaa tcaatcactg ctggcgaatt gcgtgctaaa aattctcgtc gtttgatcga
    16321 aacttctgta aaaggtatgc ttcctaaaaa cactttaggc cgcaaacaat ttacaaaatt
    16381 gaacgtatac ggtggagcag aacatccaca tgcagcacaa caaccagaag ttttagacat
    16441 cacaaactta atctaatagg agggaatttc attggcacaa gttcaatata tcggcacagg
    16501 ccgtcgtaaa aacgcagttg ctcgcgtacg tctagtacca ggaactggca aaattactgt
    16561 aaataaaaaa gatgttgaag aatacattcc acatgctgac ttgcgtgaag taatcaatca
    16621 acctttcgct gttactgaaa caaaaggcgc atatgacgta tttgttaacg taaacggcgg
    16681 aggatacgct ggacaatcag gagctatccg tcatggtatc gctcgtgcat tgctagaagt
    16741 tgatcctgac ttccgtttag ctcttaaacg tgctggctta cttactcgtg acgcacgtat
    16801 ggtcgaacgt aaaaaaccag gtcttaagaa agcacgtaaa gcttcacaat tctcaaaacg
    16861 ttaatatcct ttatatatca acgtttctgg cacttttcaa ttatttggaa agtgcttctt
    16921 ttgtttttaa gaacacattt aagaacacat atcaatcaca cttattcaat cctctctgaa
    16981 aagcttcaaa aacagcctta tcaactatat ctggtgtgtt cacatatatc tttgttgtag
    17041 aaatatctga gtgtgttaaa gcatttgaaa tttgattcat atctgctcca ccttgtctag
    17101 ctatagttgc ataagtatga cggagtttgt gtggagataa atgaactaag tcaggatgcc
    17161 ttcttttaat tgaattgatt cgatagttta ggtaatcggc atgaacagga caatttactt
    17221 ctccagatgg tttagtataa gtaaagagat actgatctaa cgtttgctta atttttaatt
    17281 tcaacaattg atcagcttgt accgatttcc attcacttag caatgtcatt acaacttctg
    17341 gtacttgaat gacggtatct tttcgacctt ttgtaaactt ttttctttga tatttatcta
    17401 aagtatgttt aagacggacc gtttgttttt caaaatcaat atatttccat tgcaaagcgt
    17461 aagtctcact ttttctatca ccaagataca gggtaatcat aaaaagtgtg taatcatgaa
    17521 aagtaagtaa atgattatta aaatcatcat ttactgcttc tatccaatta gtaagttctt
    17581 ttgatgatag tgcttgcttg ccttcttgaa ttcttttttc ttctaaagca ttttttttag
    17641 gtgcagtgat actttgaata attttagtag ttcgattaaa ttcgatataa tttagtattt
    17701 ctgcaatatc gaacatggaa cgaacat
//
LOCUS       DARDHA010000045        17368 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000045 DARDHA010000000
VERSION     DARDHA010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.045"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            197..634
                     /locus_tag="SDA10_001235"
     CDS             197..634
                     /locus_tag="SDA10_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240158.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            729..1613
                     /locus_tag="SDA10_001236"
     CDS             729..1613
                     /locus_tag="SDA10_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEG4240159.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1837..5004
                     /locus_tag="SDA10_001237"
     CDS             1837..5004
                     /locus_tag="SDA10_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240160.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            5024..5569
                     /locus_tag="SDA10_001238"
     CDS             5024..5569
                     /locus_tag="SDA10_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HEG4240161.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5597..6052
                     /locus_tag="SDA10_001239"
     CDS             5597..6052
                     /locus_tag="SDA10_001239"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HEG4240162.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            6049..6807
                     /locus_tag="SDA10_001240"
     CDS             6049..6807
                     /locus_tag="SDA10_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG4240163.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6893..7633)
                     /locus_tag="SDA10_001241"
     CDS             complement(6893..7633)
                     /locus_tag="SDA10_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4240164.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7694..8947
                     /locus_tag="SDA10_001242"
     CDS             7694..8947
                     /locus_tag="SDA10_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG4240165.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9269..9496
                     /locus_tag="SDA10_001243"
     CDS             9269..9496
                     /locus_tag="SDA10_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEG4240166.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9619..9945
                     /locus_tag="SDA10_001244"
     CDS             9619..9945
                     /locus_tag="SDA10_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEG4240167.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            10013..10726
                     /locus_tag="SDA10_001245"
     CDS             10013..10726
                     /locus_tag="SDA10_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEG4240168.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            11036..11887
                     /locus_tag="SDA10_001246"
     CDS             11036..11887
                     /locus_tag="SDA10_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG4240169.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            12008..13873
                     /locus_tag="SDA10_001247"
     CDS             12008..13873
                     /locus_tag="SDA10_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4240170.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13921..15330
                     /locus_tag="SDA10_001248"
     CDS             13921..15330
                     /locus_tag="SDA10_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEG4240171.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            15438..16139
                     /locus_tag="SDA10_001249"
     CDS             15438..16139
                     /locus_tag="SDA10_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4240172.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16298..16777
                     /locus_tag="SDA10_001250"
                     /pseudo
     CDS             16298..16777
                     /locus_tag="SDA10_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         5802 a         2914 c         3719 g         4933 t
ORIGIN      
        1 gaactattta aatcaataaa actgttcatc taaatctttt tattagaact aaatcaacag
       61 tagtttaatt actacttgat ttagttcttt ttattttaaa aatttttttt acttagaatc
      121 cagcgaccct agatattttt ctgtataatc ttaaataaag aaaacaatat caaaaaatgc
      181 acgtagaagt agataaatgg agtggttttg caaatttctt ttatttctag aagttattca
      241 cgtatacgga tttgtttttc tcacaaataa aaaggcagga agtacaatgg atcatttgta
      301 tataaaatat attcatcaat tttccgtttt ggtgttaggc acactaatta tagcggggat
      361 tgtcgcattt attattcaag gccgtgacat attaacagta catcaagagt ttttcagtta
      421 tgcatttggt attcctctga tcgctataag gtctgataac aaaaagtgga agaaacgcta
      481 ttacattctc ttgctatttt tgtgttttta tctgccacct aagattttta ctttattagg
      541 tatacaatgc ctatcatttt cttttatttc ctatatcatt gttttaattt tcctcagttt
      601 catagtatgg atgttttatc ttgtcaaaaa ataaatgaat gtttcatgaa cagtacttca
      661 tcacagccta atttttttct ttatgctatc gttatattaa agaaatcaat cagaagggaa
      721 agaaagtgat gaaaaaagtt ttattagtgg taaaccccag ttcaggcggt gaacaagcaa
      781 aggaatttga gcaattagcg atagcaaaat tggaatcggt gtttgatgaa gtggtcgtct
      841 tgcatacaaa aaaagcaggg gatgcaaaaa actttactcg cgaagcggct acggaagggt
      901 atcatagtgt gtttgtcatg ggcggagatg gaacagtcaa cgaaggaatc agtggaatcg
      961 ctgaacaaga acatcggcca aatttcggat ttttcccatt gggtacggtg aatgacttgg
     1021 ctcgagcatt agggataccg ctagaacctg aggaagcgat caatcatttt tcgattgaat
     1081 cagtcaagcc attggatatt ggaaaaataa atgacgacta ttttatgaat gtcgtcgcaa
     1141 ttggctctat ccctgaatca atcaatgatg tagatgcgga gaagaagacg aaatttggaa
     1201 aactagctta tttcatgtca ggaatcaaac agttagcttc cacacaatcc tactcgtttc
     1261 acgtagaagt tgatggaaag aaggaagaaa ttgagagcag taccttgctg ataggactaa
     1321 ctaactcagt cggcggtttt gaaacattac ttccaaatgc aaaagtggat gatggtaagc
     1381 tccatttggt ttatttaaaa gattcttctt tgatcgatac gcttaaaaca ttgccagacc
     1441 tattaaaagg agtagatgaa tctacgaaaa atctagccta ccttacttgt gagcaattag
     1501 ttgtgtcatt agctgatgag gaagaattga cgacgaatgt tgatggagat gaaggggata
     1561 aattacctgt gacaatcaaa gtattgccct cacatttaca ggtttattgt taaaatggag
     1621 gttgtgaccg aggagttggc gggagaacac cgcttgttcg ggataaggga ctgtgacaag
     1681 acttttgtca cagccttttt tttgtacaaa aaagaaattt acacctaata gaaaaatttt
     1741 tccataaaaa tacatatttt aaatatgaaa gtacaattgg aaatagatta aaacgataaa
     1801 ataaaattgt caaaacgtca gagaggatgg tggagaatga atcaatgtgt aaaaaagctg
     1861 attgcgtcga ttttgtttgt cttccctctc ttgatggtga catccgtttt tgccgatgta
     1921 gagaaaacaa acgaatcaac gcaatcagag aatcaaagtt ttgacaagac tactttttca
     1981 gaagaatcga ttacttctga aactacagaa tctcaaagtg aggcttctac taataaaagg
     2041 caagaagaaa ataagacaag tgagacctca gaagtagctg aacaaacgtc tgatagcagt
     2101 gaagcggcac tggcaagagc tgtttcagcg gatgggtaca gcgaagctgc cacaacagaa
     2161 gaactggcgc agcttctcgc agacgaatca gtaactaaga tccgactgat tcagcctttg
     2221 actttagaca gggaattaga gatcaaacga gatatcgtta ttgattttgg tggattcgct
     2281 cacaatttcg gcacacacca tatttatatc aatgagatac ctcagtcgat cgagttccag
     2341 aattttaaag gaacagctgc acatccaggt ggattgacac caaatatcga tggaaatgcc
     2401 attatcattg cttatatgac cgatttttgg ggaagcagat atcattttac gggagaaata
     2461 aaatttaccg ggacacttga tttgtatgat gggtccaaat taggactgat ttacgctcct
     2521 cgtgcaactg tcacattaga tggtgtttcc ggtgtgttgg atgtccaacc agtgaaggaa
     2581 ggactgaatg ccgctccggg taaagcctat ttttgtagaa gctatcaatt gaatgtgatc
     2641 aatggtagtc aactttatgg accgtatcta gggaaattct acggttttct agattcagga
     2701 gatgagactg gtagtggaaa aacagcgcca ggaatccata tcttaagcgg ttcaaaggtc
     2761 tctcttgatt atgatcgggc agatggcgtt agcgatgaag gagaggcagt cgatacgcta
     2821 cctggtaatg tcatgtttga agtcagtggt tcgggaagcg aattttcggt aaatacaaag
     2881 atcaatatta ccaatgacaa caatcgcgga atcatccaaa tgagaggttc aggatcgaaa
     2941 gtcactgtat cgaatggtgg gaaaatcacc atcaatacag caactacggg cggatttcgc
     3001 ttgcaaggag attctagtca aatccatgta tcaagtggtg gacaaatctc ggtcaaaatg
     3061 gctggagatg acgatcaacc cgggaacaat gggatgcgtt ttgtaggaaa aggcctttct
     3121 ttgatcgtag aaggagccgg atcagttatc gacatcgata agcagagtgg gcgttccagc
     3181 gcagttcgtt ttgagaatgg tacgcaaaag ctgacagtaa ccaatggagg ctcattgagg
     3241 gtaagaaata caggtgatgg caaatcttac gtgaacagac tcaatcaagg aaatcaagcg
     3301 atccaatttt atgacgcttc tggttttcta gaaagtccag gttcagctga ttttacagtc
     3361 actggagaaa aatcaaatat cgatatacgc gcagattccg gagcaactgt agatacaact
     3421 ggagatattg atttaaattt tacggcaggt gaaaaaactt atttagtcat ggtcgggaaa
     3481 acaggggaag ataagaccgg catcatcagt ggggatgtgt tgaatgtgga cttagaatct
     3541 cctacctatt ttgatttcca aaacaaacgg attggaagcg agaccaatgg cataggcggc
     3601 tgggtattcc aaggaaatgg agattcttct ttaaagatcg cccattcaga aatcgcttta
     3661 tggcgaaaaa caggcgcaac attcgataac tttgaaaaag acccggatta tttttctgat
     3721 atgacagact tatcgatcaa tgggaccgac ttaggcaatt tcgtatcaag ccctagtgca
     3781 ggtttaacaa atgaatttgc tcagacaggt gccggaatga aaaattataa tcgaatcagt
     3841 gccaataatc agctcccgat gattgaatcc ttaagagtgc caacgaatgc agataaaaaa
     3901 atctatgggc atgtcgttgt tcccgaaggt gtcgaggcaa agccaagaga tgcttggaca
     3961 ggcgaagtag aagtgacttt acgtattact tacgcagata cgacgaaatc atctgtagaa
     4021 attactgctc tcacgcaagg acaaacagat gaaaatgaag gggcaggata taacccatgg
     4081 ggagaaggcg aacaaggcgg cttgttcgaa gttcaagtac ccaatgatga gagattaaaa
     4141 acggaggatc aagtgcaagt tgttgctgct aaaaaagtaa gaggtggcca agaagtgaca
     4201 gaactggact ctgccaaaac gacagtcgat gtgattcctc ctgaacctgc taatttaagt
     4261 accacattga tcaatgcagc ttctcgcacg atcacaggta caggtgaagc aggagccttg
     4321 gtttcattga aaaaaaatca agctgttttg tcagaaacaa ctattgatga tacaggggaa
     4381 tttgttcttt ctatcccaga aaatcaactt tttgcaggag atgtattaga aattgtatta
     4441 aacgaccaag caggagaagc tactgctaag ggtgtgcaga acaagccaag taccaacgat
     4501 gaaattggga atcacaatcc aagtgatacg ccagttgttt atcatgatgc cccgcctttt
     4561 gcacctgcta cgaagatcgt agtagaaggt ggtctctcat ttcttgcccc ttccaaactc
     4621 gattttggtc agatcaaagc aactggtttg acacaagaag catttggcca aatggatata
     4681 caggaaaaat tatggattta tgatcagcga gaaacgaaaa atccgtggat cttaacgata
     4741 aaattatctc agcctttcac aaatgatgca ggattttcta tgcaggatgt cctatatttc
     4801 aagaaaggag aggagtatca attagtgaat gaagaagaac tggaaattgt cagaggacaa
     4861 aattcaacga atgtgcaagc agattattca gaatatttaa ataacggtcg ggcgtttaaa
     4921 cttgtcttaa ccaaagaaaa tcagatagct ggtaattact cagcagagat aacatggaca
     4981 ttagcagatg cgccgacaga ataaagatca aggaggatga ttgatgaatc gaatatggaa
     5041 taaaagtcat cgaaacaact gctctttact tatgatcctt ctccttggat tcatcctttg
     5101 gcagacacct gtccatgcct ccgaattcaa ttttgcggta gattttgaat taccggctaa
     5161 tcagattgat aagaacaaaa catatataaa tgtaaggatg gagcctggga aaacacagga
     5221 aatcccctac acggtcttta accgaacaga taaatcagta acagttgaga cattgatcca
     5281 ctccgctacg acgaattcaa acggtgtgat cgaatatgga gaatcaaaaa gtaaatttga
     5341 tgaaacatta agttatgata tgaaaaactt agcaaaaaca gtgggagaaa taactgttcc
     5401 tcctcaaagt gcggtcactg agacactttc actgaaagcg ccagaaaaag agctggctgg
     5461 tgtggtcgca ggagggatca cgtttcaaga aaaaaaagag aacgtttcta acaaaagtac
     5521 agttggtgtc gtcaatcagt tttctcaagt gaaagcaatc ttgatttgaa atagcgaaaa
     5581 agagtcttcc ccagatttga aattaaaaaa agcttatgcg gatcaaagcg atggaagaaa
     5641 tgtgattaag gcgaatcttc aaaatccaaa tgcttcttat atgttcggct tgactacatc
     5701 ggttgaaatc tacaaaaaag aaacaaaaga gccttacctt tcaataaaaa aagaaaaaat
     5761 agatgtagca cccaatacaa attttgattt acttgtccca ttagaaggaa aaaaattagc
     5821 tccgggacat tatcaagcgg ttgtgaaggg tgcttggaaa gaaaaacgct ggaatctcca
     5881 taccgatttt gaaataactg atgatgcagc ggaccaattg aatgaaaagg atattgattt
     5941 aaaaaatcag ccagaaacat ttgactggcg atggctgata cttgttggag tcattcttat
     6001 tggcttattt gctggattat ttattttaaa agaaagaagg aaaaagaaat gaaaaaaaca
     6061 attgcattaa gtaccgttct agctggaagt ctattattat gtacacaaag tgtttttgct
     6121 gagaataaga gctcagccac tacagaagga acgatttctg tgaccaaacc agatgctgca
     6181 aatcctgaag acgttgttcc agttcctcct gttgatccaa atgatcctga tactcctttt
     6241 acacctgacg aaccaaatca gggaacatcc ggattactga cgattgacca agctccagat
     6301 cttgattttg gaacaatcaa gttaggtaca gcaaaaacag tatatgctgc attacaaagc
     6361 ggaacggaca gtactggagc acaaacacaa gtacagaatt tcgtacaagt aacggataaa
     6421 agcggaaatt atgccggttg gaaattatca gtcaaacgaa cagaatttat tggtacaaca
     6481 tctgatacga ataaactaac tggttcaaag gtgaaattca aaaacgccgt gattcgaaat
     6541 agcgcaacta atagcagtgg aatgccatct accgtttctg gaactggcgc tgaaggaatc
     6601 gaaatcccag cagatcaggc agtagatcta gtaactgcag gagcaaatga agggattggc
     6661 acatggattt attcattagg agagaatgtg gaacaaggga gacaatcagt ggaattattt
     6721 gttcctgtat ccaattatgc tgtagacaac tacgtttcaa cattcacatg gtcgttaaca
     6781 gatgctccta cagaagagga agcgtaaaat catatcaacg atttcaggga gtggagccga
     6841 ttcaaggccc actccctgaa atctgctatg cttacgtctc agctcttatt catcaactcg
     6901 aagcagtttt ttggaagtca tggaaacgtt cctttgtctt ttcaaagatc tttcgaccaa
     6961 cgagtaagga gtcaccgttg aaaaattcta acacataatg agcagaatca attttctgca
     7021 attgcgataa gttgatacag taagatttta aaagtaaaaa gtaagcatac ttttctaact
     7081 ctgtcttcaa ttgttttaaa gaagtattca ctaatttatc ttctgtagtt gttttgacca
     7141 taacttggtt tttcaaacct ttgatacttt caatatataa aatttcccga ggatacagga
     7201 cgagacttcc acctttttga tcaataaaga ttttatcgac atttacgatc tgctgttctt
     7261 tccaattgat ctgttctagc acttgacgaa tttgttcaga taaattcctg tcttttctga
     7321 tatatccaat cggaaggatt ttccgattga tgatctctac tgcaaaattc gtataagcgg
     7381 tgaaaaaaac aatattacta tgtgcatttt ttttacgtat ttgttcggcg acatcaatac
     7441 ctgtgagtga ttgcttcaag tcgatatcga taaagaaaac atcgtgatta tcaatagtgc
     7501 ccaactggat caactgattg atttgagaga tagaagtgag ctgcacgata ttctcgatat
     7561 gatgattgac caattctctg atgatccatt cgctgtgaaa ctggttatct tctatgataa
     7621 aatacttttt catatgcagg ctcctattaa aaaaatatgt gtacattttg aaacaaaaca
     7681 atggaggaat aagatggtcg caacagtgac actttatttg acctttactt tgattttaga
     7741 tttaggtatc tatttattct tttcgaaaca actgctagta aaaaacagct ttggttgtat
     7801 agctttgatg gtggtgacac ttttacttcc tatccctaaa gtgagctttc cttacctagt
     7861 tggcttatta ttgatgtttc ttttttattt tactacgaaa aacctgttta tcagctgttt
     7921 gatcagtaat cttttttttg ctatcactgg actcagcttt tttctggttt ttgatctgat
     7981 ggattattta ggaaagagac atcttggaac agcaagtttt tttgcactac ttatcgcatt
     8041 tattttctat ctattagtaa aaagtataga caaacagacg agatttctca acttgctttt
     8101 tacttatcca actgaacact attttggagg tatttgtatt ttattaagct ggggcgtgat
     8161 ttacttggta ttgggaagtg ttcacttccg ctcatctaat tatctgcttc tatctctttt
     8221 gtcgatagtc atcagttttt tttctttgtt tttttccatt atgttgatca gtttccaagt
     8281 aaaagaaaga caacatcagg aatcgcttca actgtatcaa ataaatgaag aatattacca
     8341 tcatctagag gaattcaaac atgattataa aagtctactg ttttcattaa aaattactct
     8401 tcaggaaaat aaaaaagaaa cgttggaatt tttagaaata ttagaagatc attctgcagc
     8461 tatttttgaa aaaccgcaaa tacaacagct aacaaatatt gtatccccag ctgtcagggg
     8521 tgtcttttta aaatttctag aaaaagcaga atcggagaaa atcccgatcc aagtagttat
     8581 tcctgtcgaa gtgaaaacaa tcccgattga tctggttgtt tttattcgct gtttatctat
     8641 ttttatttct aacgcattcg atcatcgtgt agccgatcag agtccgatta cgatcaatct
     8701 gctggaagaa aaagagggat cacgttttag tatagcgaac aaggcaaatc caacgaattt
     8761 gacgctatcg gagatggtca aaagaggaaa aacctctaaa aaaaatggcg ggttaggtct
     8821 atacagtgcg aaaaaaatgt tggatgttta cgaaaatgca gaactaaaaa tcgaattttc
     8881 aaaacagaat cactattttt ttgtagaaat gtatctggca tctagaaaga gcaaatcagc
     8941 ctactgactt actgtcaaag gataagtttg aggaaagtct acacggagat agagacaaca
     9001 acacagcata ttcaaaagaa acagaagcgt gatgaccgtt gtcacccttc tgtttctttt
     9061 gctatgtagt taaacaaagt agttgacact tccgctactt agtattagta cgaaagtata
     9121 cttcatgata ctaagtagat aagaagtgaa aagcggaacc cacgatcgtc aaaatgctaa
     9181 ctgctttgct tacaccagat aaagggaaga gcaatatcgc ttattttagg cgtggagaag
     9241 caagcaaatc cctacgatca caaaaaaaat gctgacccag cagttaagag agttagaaca
     9301 agatgaaagt atccatcgaa aagcctataa ccaagtcccg cctaaagtag agtactcgct
     9361 caccgaagaa ggaaaatcat taagagagat tctagtagcg ttgtctgttt gggaagaaga
     9421 acgtatcgaa aaacaaaaag cctcaaggga agaagacacg ctactgcata cacatggtgg
     9481 ctacctaaat tattgatttt ctatgacatt tattttaaat aaaagaaaac aggagttttt
     9541 ttaaaagcat catctatttt ttatgtaaaa aaatggtatg attaatcaag taagaagtga
     9601 ttggaagaag gcaagcaaat gaatagtatg gaaaaagtca accagtttag agatgaacgt
     9661 aattggcgac aatttcataa cgaaaaagat ttggctatct ctatttcttt ggaagcatct
     9721 gaattgttag aactttttca atggaaaacg ccggaagaag taaaagaaac acaattagaa
     9781 cgaatcaaag aagaactggc agatgtattg atctattcat acatgatggc agacaacctt
     9841 cacctggatc tagatgagat cattgaagaa aaattgatca aaaataattt gaaatatcct
     9901 gtagaaaaaa gcgcaggtgt acgaaaaaaa tacacagagt tgtagaaatg tgtcattcgg
     9961 ctgttggaaa gagcaaaaga aaaacagtaa aatgagtctg gagggagaga aaatgaaaaa
    10021 aaaaggaaaa gcaaaaagat ggctagtcaa tatcctcttg ttcctcttgt tattagtagg
    10081 actagctttg attttcaatg aacagatcaa ggattatttt gtcagagaga caggagataa
    10141 atatgctata gcgaatgtaa caaaagaaga tctgaaaaag aataacgata aagatgtcag
    10201 ttttgacttt gatgcggtag aaccaatgac gacagaaggg gtcatgaggt cgcaaatgag
    10261 aggaacagat ctaccagtga ttgctagtat cgcagtccct tcagtttcaa tcaatttacc
    10321 ggttttcaaa ggcttggata atacgtcact actttacgga gcaggaacat tatctccaga
    10381 tcaagaaatg ggaaaaggaa attatgcctt agcaagccat cgtgcaacga accctgaatt
    10441 gctgtttaca ccgctagaaa atttagagat gggtgccaag atctatttga cagatttaga
    10501 aaatgtgtat acatacaaaa cattttttaa agaaaaagtt gctcctacag atacccaatt
    10561 actgaatgaa gtcgagggaa aagaaattgt cacactaatt acttgtggcg acatggacgc
    10621 cgtcacgcga ctagttgtac aaggagaact ggaaagtgtt acatcgataa aagatgcgac
    10681 agatgatatg agatctgctt tcaatctaga gacaaaaaca ttttaggata ctgggacata
    10741 agtcgttaca gattaaaaac agccaaagga tatgaatcga taaggaaaaa ccaatttgct
    10801 gaaaaaaaga gaaacgcaaa gaggtgcatc tctgatttgg ttgttttcta gatagacttg
    10861 tgttccagtt tttttatttt ttatatgaaa aggcttgcat aaataaaaat tcgatggtat
    10921 gatgattttg aataagaaat ggattgttac tgtacggcag gcaaaaccag aattcagcta
    10981 agaaacagta ggcgaattct aggttttttt atttttcttg agagggcgaa gcaaaatgaa
    11041 aattgataaa attttgaata acaatgtcgt catttcgaaa aatggattcg gggaagaagt
    11101 cgtttgtatg ggaaaaggac tagcttttca aaaaaagatt ggggacgaga tttcgccaga
    11161 agcggtccaa aaagaatttg ttttaagaga ttcttttgct aaaaaccaat tccagcagct
    11221 gatggctgat attccggcag aggagatcga atgggtcaaa caagtagtcg aactggctga
    11281 agataaacta aaagttgaat tttcgcctaa tatttatctg acattgaccg atcatcttca
    11341 ttatgcgatc acacgagcaa aagaaaatat ccaactgcct aatccattat tatttgaaac
    11401 aaaaaaattt tatccgaaag aatatcaagc agcgaaagag gctcttttgt tcgtgaaaca
    11461 aaagacgggt gtggaacttt ctgaggatga agccggtttt attgcctttc attttgtgaa
    11521 tagccaacaa ggaaacgaag acatgcaagt gacgatgacc gctaccacaa tggtaagaga
    11581 tatactgagt atcatcagta aattcttcgg catcgtgttc gatgaagaat ctttgaatta
    11641 tcagcgtatc attactcatc tgcagttttt tgcccagcgt tatctcaaag gggaaggatc
    11701 tgacgagcaa gatgaatttc tctacgcatt agttcaagga aagtatccaa aagcgtttca
    11761 ttgtgtcgaa cggatcaatg agtatcttcg acaaacgcaa aaacaggaaa tggggatcgc
    11821 agagcagatt tatttaacga ttcatattca aagagtagta agtgagaaaa aaagcatcca
    11881 atcataagga ttgttactgt atggcaggca aaacctggat tgaaaaaata ggttcacgaa
    11941 tgtttgtgac tgtttttttc aatctgggtt tttatttttt aaataaaaaa gaaagaagga
    12001 aaaaacaatg gataatcaag cagttggtcg tcgtgtatgg gaagcggtag gaggacaaaa
    12061 gaatgtcaaa agtttagtac attgtgctac gcggttgcga ttcagattaa aagatgaatc
    12121 tttagcagat acgcaaaagt taaaagaaga cccggacgtg atccaagtcg tacaaagtgg
    12181 cggtcagtat caagtagtga ttggcagtaa tgtagcagat gtttatcaat cgatcgtgga
    12241 tgaggaaggt cttgcagttt cggaggatgg aaaagaagag tccaaaaata ttttgaatcg
    12301 tctgatcgat attatctcta gtatatttac accttttcta ggggcgatgg cagccgccgg
    12361 ggtattgaaa ggattcttat cattggcaac ggtgatgggc tggctgacag ctgatagcgg
    12421 tacttatcaa atattgtttg ctgcagcaga tggtgtcttt accttcttac ctgtcatgct
    12481 tgcttttaca gcagctaaaa agttcaaagc caaccagttt ttagcagtag caattgctat
    12541 ggcacttgtg tatccagcta tcacagcagt tgctggtgcg ggagaagcta tcagtttctt
    12601 tgggatccca gtgattcttt caccatctgg ttatacttct tctgtcatac caatcattct
    12661 agccgtatgg gttcaaagta aattagagcc attcgtaaaa aaagtgattc cgcaatttct
    12721 gcaaatgatt ttagttccgc ttgtcgtttt agtagtgatg gttccattaa catttcttgc
    12781 tttaggacca atcgggacgg ttgccggtaa tgctttaggc ggtctcttca actcgattta
    12841 tggatttagt ccaatcgtag caggtctaat catgggaagt ttatggcaag tattcgtgat
    12901 gttcggcatg cattggggat tcgtaccgat catgtttttg aatatcgaac aatatgggtt
    12961 tgatgtattg atgccaatgc ttcttccagc catcttggca caaggtgggg ctgctttagc
    13021 tgtagcattg cgaacaaaag atacgaaact tcgagcatta gggatttctt ctacagtgac
    13081 ttcgttattt ggaatcactg aaccaactgt gtatggtgtg acattacctt tgaagaaacc
    13141 attcattgcc gcttgtattt ctggaggtat tggtggagct attatcggat tttccggagt
    13201 aaaagctttc tccagcagtt tggtcagctt attgacgatt ccgactttca ttaatacggt
    13261 ggatggtgta gagtcaaatg tgacagtagc agtaatagct acaggaattg cttttgttct
    13321 tgcattcgta ggtacattga ttttaggatt tgacgaacaa acacaagaca atcaattaga
    13381 aaacaaacat gcgaatgcag gaaaaccgat tacttcagca cggcatacct taaaaagtcc
    13441 tttaacgggt aaagtactgc cattgagtga agtaccagac caagtttttt cttctggcgt
    13501 gatgggaaaa ggtatcgcta ttgatccaga agtaggagaa ctagttgcac ctgcagacgg
    13561 agagatcacg actatttttc caactggaca tgctgtaggg atcacaacga ctgacggagc
    13621 ggagattttg attcatatcg ggatggatac agtagaatta aatgggaatg gctttgagat
    13681 cttagtcaaa caaggggatc tcgtcaaagc aggagattta ttgattcgtt ttgacatcga
    13741 agcaattaga gcagctggct atagcgtaat cactccagtt gtcattacga atactgatgc
    13801 atttgccgat atacttgaat tagaccaaaa agagattatt gcaaacgaag atgttctggc
    13861 aattgtaaaa taaggaggaa atcaggatgc aatcacgttt ttcagaaaaa tttttatggg
    13921 gtggagcaac tgctgccaaa tcaattagaa ggggcttatg atcgagacgg aaaaggacta
    13981 tccgttgcag atgctatgcc tggaggtaag cagcgctttg cgattatcgg cagtgaagaa
    14041 tttgactgga cgattgatca agaaaaatat atttacccga atcatcgagg aatcgatcat
    14101 tacgatcgtt tcaaagaaga tcttgccttg tttgcggaaa tgggattcaa atgttatcgt
    14161 ttttcgattg cctggacacg aatttttcca aatggagacg aaggtactcc taacgaagcg
    14221 ggattagaat tttatgatca gctgattgat gagtgcttaa aatatgatat tgaacctgtg
    14281 atcacgattt ctcattacga aatgcctctt catttggcaa aagaatacgg tggctggaaa
    14341 aatcgaaaac tgatcgactt ctatgaacga tttgctcaga cagtattaga gagatacagc
    14401 agtaaagtca aatattggat gactttcaat gaaatcaatt cggcctttca ctttccagct
    14461 ttaagtcaag gattagtaaa aagcaatgga gcaggagagt accaaaatat tttccaagca
    14521 tggcataatc aattcgttgc aagtagcaaa gccgtaaaaa tcggccacga attgcgttcg
    14581 gatatccaaa tcggctgcat gattatctat gcaacaactt acagcatcga tgccaatcca
    14641 gtcaatcagg cagcaacaat gatccaaaac caagaattca attttttctg cacggatgtc
    14701 caagtacgtg gagaataccc tgcatacaca gcgcgtaccc ataaaaaata tggagtcgat
    14761 ccggaaaaat tagagcaaac ggaaaaagac ttccagttgc taaaagaata tccagtggac
    14821 tacatcggat ttagttatta tatgtctacg gcaatcaatg agactgatcc acaagcagca
    14881 acttcagaag ggaacttgct gggaggagta aaaaatccgt ttcttgaggc aagtgaatgg
    14941 ggctggcaga tcgatccaga agggctgaga atcgcattga atgaattata caatcgttac
    15001 cagaaaccat tattcattgt tgaaaacggt cttggagcca ttgatcatat ggaagaggat
    15061 ggcacgatta ttgatgatta tcggatcgat tatttacgtc gtcatatcga agcgatggct
    15121 aatgcagtag cagacggtgt agatttgatg ggctatacgc cttggggatg tatcgatctt
    15181 gtcagtgcat caacaggaga aatgagtaaa cgttacggct ttatctatgt ggatctagac
    15241 gatgaaggaa atgggacact gaatcgatca aagaaaaaat cttttgattg gtataaagaa
    15301 gtaattgcct caaatggcgt aaacttgtaa ttaaagcagc tgaatgaaaa agaacacact
    15361 tctcacatgt gatttgtgtt ctttttttat tttttatgtg ctattctttt agtaatagta
    15421 aagcaaaggg tgacgttatg aaaaggacac gtgtactata cttggaagtc gcagataaaa
    15481 ttaaagaaga tattttctca ggaaaatatc cagtcggttc gatgttgccg acagaaagtg
    15541 aattagaaga actgtttcaa gtcagcaaaa tcactgtccg aaaggcaatc gaactgctgg
    15601 catcagatga atatgtcgca aagaaaagtg gtcgtgggac gacggttcta agcaatcgtc
    15661 cttacaatcg attatccaaa gcggcaagct ttacgcaaat cttggagaac tcccgatatg
    15721 aagtccggaa agaaacgctg gatttgcaat tggtccatat aaagaaggaa gatcctttgt
    15781 tttccttttt cggtgagcaa gctgtttgtt ttaagcgctt atattatttg aacaatcagc
    15841 cttatattta ttttgttcat tatcttccac aagaaatgat gcaattcagc agagaagaat
    15901 ttggcaaaga atctttgtat cgtttgctga cgaaaaaagg atatactatt gactcatttt
    15961 ctgatgattt ctcggcagta tttttaacag aagaagaaca aggaattctg aaaacagacg
    16021 cacagatcgg catcaaacgg atacggaaat cgtctacgca aattggggaa atgattgaat
    16081 attcggaggc ggtctaccat acggagctgc atccttacca tattgattat gaaacataaa
    16141 taaaaaaaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag ccaaacaaaa
    16201 atagcttctc atatttttcg tttggttagg cttattaccg cagatacacg gctgtttctg
    16261 gattaccatt tattctggat aagggggtgt ggcacgattt tcagaaggaa tctatgaacc
    16321 aatcactgta ttgattttag cttcgatggt aaccccattc ggcttgacga tcgcttattt
    16381 cttagggaaa atcattcgta aaaatatact caatcgtcaa gaaatcgata cattgaagac
    16441 cgcttttcct atggggatct gtcagatcac agagggatgt ttccctatcg tattgaacga
    16501 cttggcacgc aatgtcatag caacgggagt cggtgggaca gtaggtggcg gattaagcat
    16561 gttttggggc gcagatagcc gtattccggc atcgggaatg tttgcagtcg ctacgatgac
    16621 cagaccatgg gcattcattg gtgcattgtt agctggttct tttgtcacag gaataaccat
    16681 tttattattg aaaaaaccag tagatccgaa tgcagaaatc atccaagaag aaaaagagga
    16741 agaagacata tcttgggatg atttgacaat tagttaggag aggcagaatg gattacaaga
    16801 aaaaagtaca atagatgtac cttgagacag gaatggtatg aaagaatcag agttagaaag
    16861 aatcgaatat acagattttg gtttaggagc gatcgaaact gtccagagca tcggcaccct
    16921 ccttgaaatg acgatgaagg aaaaatgaga acctttcgtt gtcgaaaggg tctggtatac
    16981 cttatgtcga aggaaaggaa acaaaaaaac tttcctgtac tattccaaaa aggaaagaaa
    17041 gttattatac agtaaaaaaa gaaattattc ttcatgctag agatcagtac acgttcgagc
    17101 ccaatatcaa acactagttc caggcaggcg caacaggtgc ggttatcact gaattttctt
    17161 ccagcagtga cgattcaagt gatattttta caaatccagc tatttatcgt taacaaaaag
    17221 atttttagga gaagctgatg aacaaaaatg atcaaatttt gatcgtttag ttcgtcggct
    17281 tttttattta gagaaacatc atttatcagt gaaacataac acgagaacaa aatgattttt
    17341 tcgttaataa aagaaacaag tatttgtt
//
LOCUS       DARDHA010000046        17340 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000046 DARDHA010000000
VERSION     DARDHA010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.046"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(28..726)
                     /locus_tag="SDA10_001251"
     CDS             complement(28..726)
                     /locus_tag="SDA10_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEG4240173.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(719..2071)
                     /locus_tag="SDA10_001252"
     CDS             complement(719..2071)
                     /locus_tag="SDA10_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEG4240174.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2294..3694)
                     /locus_tag="SDA10_001253"
     CDS             complement(2294..3694)
                     /locus_tag="SDA10_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEG4240175.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3855..4592
                     /locus_tag="SDA10_001254"
     CDS             3855..4592
                     /locus_tag="SDA10_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEG4240176.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4717..5196
                     /locus_tag="SDA10_001255"
     CDS             4717..5196
                     /locus_tag="SDA10_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HEG4240177.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5193..5960
                     /locus_tag="SDA10_001256"
     CDS             5193..5960
                     /locus_tag="SDA10_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240178.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6190..6759
                     /gene="lepB"
                     /locus_tag="SDA10_001257"
     CDS             6190..6759
                     /gene="lepB"
                     /locus_tag="SDA10_001257"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG4240179.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6863..7465)
                     /locus_tag="SDA10_001258"
     CDS             complement(6863..7465)
                     /locus_tag="SDA10_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG4240180.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7571..8040
                     /locus_tag="SDA10_001259"
                     /pseudo
     CDS             7571..8040
                     /locus_tag="SDA10_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8146..8730
                     /locus_tag="SDA10_001260"
     CDS             8146..8730
                     /locus_tag="SDA10_001260"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEG4240181.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8733..9806
                     /gene="prfA"
                     /locus_tag="SDA10_001261"
     CDS             8733..9806
                     /gene="prfA"
                     /locus_tag="SDA10_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEG4240182.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9799..10638
                     /gene="prmC"
                     /locus_tag="SDA10_001262"
     CDS             9799..10638
                     /gene="prmC"
                     /locus_tag="SDA10_001262"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEG4240183.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10631..10810
                     /locus_tag="SDA10_001263"
     CDS             10631..10810
                     /locus_tag="SDA10_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240184.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10832..11857
                     /locus_tag="SDA10_001264"
     CDS             10832..11857
                     /locus_tag="SDA10_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEG4240185.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12032..13276
                     /locus_tag="SDA10_001265"
     CDS             12032..13276
                     /locus_tag="SDA10_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEG4240186.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13429..14376
                     /locus_tag="SDA10_001266"
     CDS             13429..14376
                     /locus_tag="SDA10_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG4240187.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14510..15139
                     /gene="upp"
                     /locus_tag="SDA10_001267"
     CDS             14510..15139
                     /gene="upp"
                     /locus_tag="SDA10_001267"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEG4240188.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15278..15520)
                     /locus_tag="SDA10_001268"
     CDS             complement(15278..15520)
                     /locus_tag="SDA10_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240189.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15641..15713
                     /locus_tag="SDA10_001269"
     tRNA            15641..15713
                     /locus_tag="SDA10_001269"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15674..15676,aa:Thr,seq:cgt)
     gene            complement(15832..16470)
                     /locus_tag="SDA10_001270"
     CDS             complement(15832..16470)
                     /locus_tag="SDA10_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG4240190.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16593..17225)
                     /locus_tag="SDA10_001271"
     CDS             complement(16593..17225)
                     /locus_tag="SDA10_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEG4240191.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT         5912 a         3075 c         3437 g         4916 t
ORIGIN      
        1 tttgtcacat tccccttttt cgaataacta ggctgtttgt ccttcaagat agggcagatc
       61 caattcttta ccgtaacgtt gttctaaagc taggcggatc aaacgagctg ccaggttctc
      121 atttcttgca aggataggtc catggaaata agaaccgaag acatttcggt aaatgacacc
      181 ttcagattgg tcttcaccat tatttccttg accttgtacc actttcccca aaggtcgttc
      241 accttcacct aagaaagtcc gaccattgtg gttttcaaat ccataataag tttcattgaa
      301 ttcttcgtta tggataacga tatcaccgat aaaccggtta ttttcctgac ttaacgtgta
      361 gtgatccagt gcactgattc cttggatctt ttctccatta gctcccatgt agtagtgacc
      421 tagcaattgg tagccaccac aaatggctaa catgacgcca tcattttcga tatagttgat
      481 caaagaatct ttctttttct ggatgtcttt tgaaatgatt aattgctcaa agtcttgacc
      541 accgccaaag aaaaccaagt catatttatc agcatcaaaa ggttcatgta tactaacgat
      601 ctccgtatgg caagaaaccc ccattttttc tgctatatat ttcaacatca aaaggtttcc
      661 gttatcacca tatgtgttca tcaaatttcc ataaaggtgg gcgatattga gttcataatt
      721 agccacggtt cattcctcct ttgataaatc cttgagcagc tagttcttta cgaagctgca
      781 atacagcagt gtaagtagct aaaatataga catgctcagt tggcaattct ttgatttttt
      841 cgattacttc tggtaattct tttgtttctg tcaatttatt ttctggaatc ccggcaactt
      901 ttagacgtaa agtcatatct gtatgacggt ctcctccaga aataacagct gggatatcca
      961 tatcggcaaa gttttcaaaa ttaccatccc aaatccagct gacatcgatt ccgtctgcgt
     1021 aattggcatt taataaagcc actaaagaaa atggataagg agctagacca atcatatcga
     1081 ttacttgatt gatacctaca ggatttttga caagtactaa tgtacatttt ttcccattga
     1141 tattgatgac ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct tgtttgattt
     1201 tatcaggatc gacattgaag tactctgcca ctgcagtagc tgctaaagca ttgtagacat
     1261 tatacatacc gccaacttcg attccgtatt catgaccatc gatcacgaaa tctgctgaca
     1321 cattgtccat ccgtaccata tcagttaatc gataatccaa ttcaggacgt ttgaagtcac
     1381 aatttggaca gtaatatttc cctaagtttg cataagtgat cattttataa tgcaagatgt
     1441 gatgacaatt tggacataag acaccgtctg tattgtaatg agccatttgt tcacgatctt
     1501 cttcatggtc aaagccataa tattttctag gattgactgt ttcgatagaa ttgaagattg
     1561 gtgaatcacc gttactgata atcgttgcat ttggtgactt tgcggcaccg tctacaatca
     1621 ttttataggt cgtatagatt tctccatacc gatccatttg atcacggaaa atatttgtaa
     1681 ataagaacag ttctggtttc atatattctg tgactttgct caaacttgct tcatctattt
     1741 ctaggacagc aaactttttc tgtccttttt ttggttttgc ctgcaagaaa gtcgatacaa
     1801 ttccctgtac catattcgca ccagtagggt tggttagaac ttcatcgaat tgctggcgca
     1861 aaatattgac tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg acaacgacga
     1921 cttggtagtc ttttgccaaa ctatctagta tatgtggatc gactttcagc gctagtttac
     1981 ctggtaaact tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac gtttttccaa
     2041 ctgcaatcgc aaaatgactg cgtattccca ttatgtaacc tccaaaaacg atttgctgac
     2101 gaataaattc aactaatcat accatatgtt gaaaaaaagg ttaaattgct tttcttattc
     2161 ttaagaaaag gttacggctg aaagtgcatg aaatcggaca aaatcgcatg atttaacaaa
     2221 aaaacagctt gaaacaaaat gattgtttca agctgctgtg attttattat tccctcttac
     2281 ctatattccc attttaaccg ataataattt tctcgcttgg atattcatag cctagatctc
     2341 gtgtttcttt tggcacgaat aacatcaatg tatatagcat tccgatccgc ccaataaaca
     2401 tcagtgtagc aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc cctaaagata
     2461 aaccagttgt tccaaacgca gaagttactt cgatgatgat cgagatcagc gtttgttctt
     2521 ctgtagcaga taaaaaaacg atacaaaaga aacacatacc aagagaaagc ataaagacaa
     2581 cgactgattt tcgcacatca tcttgatcga ttctccgtcc aaaaatattg atgttatctt
     2641 cacttttgag gaaggaatat agatacaaac cgatgattgc tacagtagtt gttcgaatcc
     2701 cgccgcctac agaactagga ctgcatccga taaacatcag taaagaaaaa atgatcagtg
     2761 ttgtgatttg aaaatcaccc aaatcgtgaa tctgcagacc agcattacga gtcgtaatcg
     2821 aataaaacat cgaattgatc catttgacac tcatattgga atcttggaat aaatggtcct
     2881 tttccaatag ataaatcaag acagttcccg aaacaaacaa gataacaaaa gctaacacag
     2941 ctaatttggt aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt ttgtataata
     3001 gccattcccg gcactccatc aatacaggaa aaccaattcc tccgacaaaa atcaaaaaca
     3061 tgatcaggat caagaaaaaa tagtcatgtg caaagggctt gatggaatcc ccagtgacgt
     3121 caaacccaga gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa acggcatcac
     3181 gccaacgatc aaaatagcct ctgtaataaa aataaataga aaagaatgtc ccaaaaagta
     3241 attgaaacca gattaaaata gcaaaagtga tccgaatcag tcggacgata ccagacaaac
     3301 gcggttgatt catatccgtc atgatcagct gtcgctgttt cagtgtgatc cgtcgtttag
     3361 aaaaaatgac aaaagcagta gaaatcatca tgatccccaa accaccaact tgaaataata
     3421 tttccagcag tataataccg ttatcattga acacagaatt gatatcaaaa gtcgatagtc
     3481 cagtcacact cactgtactg attgccatga acagcatgtc cacaaaagaa acatggctat
     3541 ctggttcgcg aaaaatcggc atataaaata gaacgagtga taagaccgtc atcaatatat
     3601 aataaaaaac gatgatttgg atcgatgaga aatgattggc agcaaacttc tgtcctcgcc
     3661 gctgcagcct tcgaaagaat aaatcaacat gcacagtact atcctcctga caatagtcgt
     3721 ttctaccagt atacaagggg aattaggaat actcaaagaa attatcaaac agataacaat
     3781 acagctttcg attaaaatag aaatggctta taataaggaa aatcctagaa aaaagaagga
     3841 ggaggaataa caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa aatcaaaaaa
     3901 taaattatga tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa gaagtaaagc
     3961 ctaagatatt aatacataca tgctgtgctc cttgcagtac gcacacattg gaattcatgt
     4021 ctcaatatgc agagatcacg ttattttttt caaattcgaa tattcatcct aaaagtgagt
     4081 atttgcgaag attacatgaa caaaaacgat ttgtagacca atttaatgag aaaactgacc
     4141 atcatgttgc gctaatcgtt gatgaatacc gtccaaatga attcaccaaa atggttttaa
     4201 caaaccatct tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc ttcaatatgc
     4261 gattagatct tgttgcgcaa aaagcgcaag aactaggata cgattacttt ggaagtgccc
     4321 tgaccatctc gccaaagaaa aatgcagctt tgatcaatca gatcggaatg gatatccaaa
     4381 agatttacgg gacacaatac ttgccaagtg atttcaagaa aaacaaaggc tatgaacgct
     4441 ctttgcaaat gtgcaaagag tatgatattt atcgccaatg ttattgtggt tgcgtgttcg
     4501 ctgccaagca gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa gcttacttaa
     4561 aaacatcaac cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa cgtaagaaaa
     4621 aaataaagaa tttatggtag aaataaggta gactgtataa tttgtccgta gtaaagtaag
     4681 gaaacaagaa taaatttcaa tgaaggaaga gataagatgc gaaaagtagc acctattttg
     4741 aagccaatcg ctgtattaac aatctcgacg attgtcgcga ctactacagg tattgtgaat
     4801 agttccagct accgtgcacc aagttcaagt tctagtactg tagaagaaac aacaccaact
     4861 acttcagtct cgacatctga accaacaaca acggaaagtt tgaatgaaac cactacgaca
     4921 agtacatggg acgatacaac aactagttcg tcgtatgatg agacatatac aactgacagc
     4981 tcaagcgaaa caagtaatac ggtaccagat ggcactccat cggattcaac caatccaagt
     5041 acaagtccgg atacaagtgc tagctccgaa acgataccaa gcagtacgac tgtagatgat
     5101 aacacgcaaa atgatgatgc agcacgtaca gcacagcaaa accaagattc aggaaatacc
     5161 caaacgtata accagcaagc aggtgattct caatgacatt tttactagac cttatcaacg
     5221 gggtgcataa attccttggc tatttagaca tcagtcctaa atatcttaac cgtggatata
     5281 cgattttaag tgtgattccc acgctttatt tattgagaat tgtttatgga ctttggcaaa
     5341 accaaaacta tcttcaattt ttcttatatg gacttgcatt tctagtgctt gtttatttta
     5401 ctgttttaaa tgtattttat tactttttag ataaaaacac gaaagccgat gtcactcagc
     5461 tttttgtgaa gtatttacct gatgaagcgt tcaacatcca gacagaagaa tcgatagata
     5521 atggaaattc gatcgataaa gtgaatacac aagaggtcat ggtttcttat gacgaagatt
     5581 atcaattgaa attagccgag aatatgcggt atttgattgg aaatggtgag atcaaaacaa
     5641 atgaactcgg tcgcatggat ggatttctgg tcgatagaaa tacattgtat ccttattact
     5701 atgtcaagaa aacaggagag aaagagtatg ttctaaatat tggacgaagc tactcggaca
     5761 tggaaaaagt agggaccatt cgacttgact caacagaaga cacattggaa cctgtcggat
     5821 tgttcatcgt cggcggtgat tttgtcaaag acgggttacg ctatcatgag ccttatcgtc
     5881 tgaaattgat tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat agtcgtagcc
     5941 aaagaagaag acattcataa tgtgatgaaa cgccgtgtat cagtggttgc gataaaaacg
     6001 tttattcgga ataaggggga tgtgacacgt cttttgtcac gttccttttt tatataacga
     6061 gaaagataag acgaacacat cacttattct ttcctttcat ttataaacat tgtagaataa
     6121 agaaagtaca acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa tacaagtagg
     6181 aggtaagctg tgaaagaatt tttgaaaaat tgggggattt tgatccttgt attagcggct
     6241 attttattag ctagagtgta cgtgtttaca ccagttactg taaacgggca ttcgatggac
     6301 cctacattaa gtgatggaca acgattgatt tcatctaaga tctcaaacta tgaacgaatg
     6361 gatattatta ctacaaagga accgggggat gaagaacgga tgattgttaa acggatcatc
     6421 ggtatgcctg gagatacagt caagatggaa aatgaccagt tgaccattaa tggcaaaaag
     6481 tatgatgaac cttatctgga tgagttcaaa aaagaatttt cagaagataa gttacaagga
     6541 gaatatgctt atagctcagg attccaagca caagcagaaa gttcatccac ctttacgagt
     6601 gactttgaat atactgtgcc aaaaggaaag tatcttgttc taggcgataa ccgcttgatt
     6661 tcaaaagaca gccgaatgtt tgggttagtc gacaaagata tgatccaagg aaaagttgtt
     6721 ttccgttatt ggcctctttc agaaattaaa atcatgtgat gaaaaaataa aaagaagctg
     6781 tgacatgttt tttgtcacag cttctttttc cgaaaattcc ttcttatttc ttggagttgc
     6841 tcactttttt tgcaatctca ttttaagctt tttggctgat ggataagaaa agcgaagcgt
     6901 ttttgttcat aaatagcttt tccccacgat ggaaggtatg actagtagca gagacaaagc
     6961 ccgttttttc caatcgatta ttcaattctt cttggctaaa gccattatgg actttcggat
     7021 gactaatttg ttcattcttg tcaaagtcta cgatgatcaa ttgaccgcca ggagctaaaa
     7081 tttcataaag ttttgtaaga atattctccg tatcaggaat gtgtaaaagt acaagtgata
     7141 gaaggatgag attagcttgg acagcaggaa attcgacaga agcatcagcg tggatcgttc
     7201 ggacattttt cagatctgct gcttggatct tttcttctgc cattttcagc atagtttctg
     7261 aagcatcggc aatgatcagt tctttgaatc tttccgcaag cggtaaacta acaagcccag
     7321 ttccgcctcc gtaatccagc aggacttttt ggtggctgtc gcgcggtaga taagtagtta
     7381 gttcttcgcg aataatcttg gctaatgctt gacggtcagg ggaatcataa tggttagcga
     7441 tggaatcaaa aatatttttt gtcatcaaat agctccttac gtaaaacttt gaaattttaa
     7501 cttattatag cacgtgaaaa gaaagaaagc atgctatact caattcaaaa agggagaatc
     7561 tagaaagaat atgccgatat cgtttgtaaa agatagagag gaaaaaggaa agtgtgtccg
     7621 agaaatatta ctagacttgc ctgagtggtt tgggcttcct gaatcaacgg aaaaatatat
     7681 cgaggaatca agtaaactac ctttatggtg tgaaaaaaga aaagaagaat atttaggctt
     7741 tatcactctt tcacagacaa gtgaagatac tgccgaaatc tattctattg tatgggaata
     7801 aaaaaagact atcatcacca aggattagga acaaaacttt ttaaagaagc agaaggttac
     7861 gcagcaaaac attacaaata tttgcaagtg aagactgtag acgaggggca ctactctata
     7921 tatgatcaaa ctatttgttt ctacgaatca ttaggatttt caaggttgga agtttttcca
     7981 aatttatggg atgaatggaa cccatgttta gttcttgtca aaaaattgga acaaaagtga
     8041 gcaatagatt taatactaat ctgtgcttga tgaaaattct cactgtaaaa aatgctgaaa
     8101 atcaagtata ataccatagg aacaaactgg aaggtgaaaa agagcatggc acaactattt
     8161 tttaagtatg gcgcaatgaa tagcggcaag acgatcgaga tcctaaaagt cgcacataat
     8221 tatgaagaac aaaataagcc agttgtctta atgacaagcg gtattgatac aagagatggt
     8281 ttaggcgttg tttcaagccg aatcggactg aaacgtaatg cgatgccgat atttgaagaa
     8341 acagacgtat ttgacgtaat tcagaaaatg gaaagcaaac cctattgtgt gctgatcgat
     8401 gaagcgcaat tcttaaaaaa gaaacatgtc attgctttta cgagaattgt ggacgaattg
     8461 gatattccgg ttatggcatt tggtttgaaa aatgattttc gaaacgagtt atttgaagga
     8521 tcaaaatatt tattgcttta tgcagacaaa atcgaagaaa tgaaaacaat ctgttggttc
     8581 tgccacaaaa aagcaatcat gaatttacat tatattgatg gaaaccctgt ttatgaaggc
     8641 gatcaagtgc aaattggcgg aaatgaagct tattaccccg tttgtcgaaa acattacttt
     8701 cacccatcaa tgattacaga aggagaataa gcatgtacga tcaattacaa gcaattgaag
     8761 accgttatga agagcttgga gaacttttaa gtgacccaga agtgatttct gatacaaagc
     8821 gttttatgca gctttcaaaa gaagaagcaa acacgcgcga aacagtcgaa gtttatcgtc
     8881 gttataaaga agtagtacaa ggaatcaccg atacagaaga actgcttggt gaaaaactgg
     8941 atgatgaaat ggctgaacta gcaaaagaag aattatctga attaaaaaaa gaaaaagaag
     9001 tcttagaaga aaaaatcaag atcttgcttt taccaaaaga tccaaatgac gataaaaata
     9061 tcatcatgga aatccgtgga gcagcaggcg gagacgaagc agcattattc gctggcgatc
     9121 ttttcaatat gtaccaaaag tatgctgaag cacaaggctg gaaaacagaa gtgatggaag
     9181 ctaacgttac cggaatcggt ggatataaag aagtgatcat gatgatcacc ggagacaacg
     9241 tatattcgaa attaaaatat gaaagtggtg cgcaccgcgt ccagcgtgtt ccttcaactg
     9301 aatcgcaagg gcgtatccat acttccaccg caacagttgt cgtgatgcca gaagctgaag
     9361 aagtagagat cgatattgaa gacaaagata tccgtacaga tatctatcat gcttcaggtg
     9421 ctggcggaca gcacgtcaat aaaacagcat ctgctgtacg tttgactcac ttaccaacag
     9481 ggatcgttgt tgctatgcaa gatgaacgtt ctcaaatcaa gaaccgtgaa aaagcaatga
     9541 aaatcttgcg tgctcgtgtg tacgataaga tccagcaaga agcacaaagc gagtatgatg
     9601 ccaatcgaaa atcagcagtc ggaactgggg atcgttcaga acggatccgg acgtataatt
     9661 tcccgcaaaa tcgtgtgact gatcatcgta tcggattaac tatccaaaaa ttagatcaga
     9721 ttttagcagg gaaattagat gagatcgttg atgcattgat catgtatgat caaacatcaa
     9781 aactggaagc tatgcaaaat gggtaaaatg acctatcgag aagtcctgtc acgggcttct
     9841 tcttttttag aggatcatgg gaaagaagca tatagtatcc aatttttatt tttggaacga
     9901 aaacaatgga aaaaacttga ttggctcctt catatgaacg aagagatttc agaagaagaa
     9961 cagcggttga tcgaaaccga tctgcgactt ctgcttgcag atcatccacc tcagtatttg
    10021 ctaggatatg cagattttta tgatcatcgg ttaaaggtaa ctgaagcaac actgatccct
    10081 cgaccagaaa cagaggagtt agtcgaatgg tgtttagacg agacgcctgg tgtaccgtta
    10141 gaagtgatag atattgggac agggactgga gcaattgcta ttagtttgaa agcagcaaga
    10201 aaaaattggc atatatctgc tgtggatctt tcagaagaag cattagaagt agcgaaagaa
    10261 aatgctcaga aagaaggaac gaaaatcagt ttttatcatg gagatacatt aggaccagta
    10321 atggatcagc aatttgatgt gattatttct aatccgccgt acatcagtcg gaatgaatgg
    10381 gaactgatgg atgaaagtgt ccggtcattc gaacctaaaa tggccctttt tgctgagaac
    10441 gatggattag ctatttatga aaaaatagca gttgaagcac caagcgtact aaaatcaaat
    10501 ggaaagattt ttttagaaat cggatttcgc caaggtgaag cagtaaaaaa aatatttcag
    10561 caagcatttc cagataaaaa agtggcgata aaaaaggatc tttttggcaa tgaacgaatg
    10621 atccaagttt atggttgaag ttgtggatga aaaatttttt atttgttttt ttattataaa
    10681 aaaacactta tttgacaagg atgtaaacga ctttttggtt tatcaacaag tttatccact
    10741 attgacaaat agattatcca caaacagtgg aaaacttgag gataaactca gacaaaataa
    10801 gaatgtatga aaaaggggtt gtggataaac tgtggaaacg aaacgatatg atgaaacaga
    10861 attgcaagag gcagccgaag cactgaaaga aggagaactt gttgcttttc ctaccgaaac
    10921 agtatacgga ttgggcgcca acgctctttt accaaacgct gtcaagaaag tgttttctgt
    10981 aaaagggaga ccgcaagata atccattaat cgtccatgtc gcctcttttg aacaagtaaa
    11041 agagtatgtg gacaactttc atccggcagc agagaagatt gtgaagaact tctggcctgg
    11101 accattaact ttgatattta agatcaaaaa agacactttg ccctcagttg tgacgggagg
    11161 attatccaca gctgcatttc gtataccgga taacaaaaaa acactagaag taatcgaact
    11221 ttccggtgtt ccgcttgtcg gtccaagtgc aaatacgtcg ggaaaaccaa gtccaacgac
    11281 tgcagatcat gtatatcatg atctacaagg aaaaattacc gggattattg atgatggagc
    11341 tacgcgaatt ggtgtagaat ccactgttct tgatttaagt gatccaacag ccatgcctat
    11401 gattttacga ccaggtgcag taacgaaaga acaaatcgaa gcagtcatcg aatctcctgt
    11461 tgctattgat cagcatcttg taaaagaaaa tgaaacacca aaagcccctg ggatgaaata
    11521 caaacactac tcacccgata cacgtgttct gatggtcaga gatggcgatt ggtcaactgc
    11581 agttcaatgg gcaaaaaata agaagatccg tgcaggtgtc atagcaagcc ccgaaattgc
    11641 ggatcaagtg cggacagata ctgcagctgt ttatatgtat aacgacaact cagtagaagc
    11701 ggcggctaaa ggattgtttg caggtctaag aggattagat gaaccaacac ttggattaga
    11761 tttgattttt gtacaagtgt atccagaaac tggattgggg aatgcttaca tgaatcgctt
    11821 gaaaaaagca gcaggacaaa attattttga aaaatgatca attggttatt gtcgcattct
    11881 tttagtatga tacaatgcta tcaagtaaat cttctatcag tgaacgaaaa actaagcgta
    11941 actaatcgta atagaagaga tacgaatatt tggcaatttg tttttgatgg gaaaaataaa
    12001 attgtaggaa aataaaaagg agctgttgtt tgtggtagat tacaaaacgt ttgaccctga
    12061 tctttgggct gcgatcgcca aagaagaaga aagacaagaa cataatttgg agctgattgc
    12121 ctctgaaaac tttgtctccg aagcagtgat ggctgcacaa ggaagtatct tgacaaataa
    12181 atacgcagaa ggttatccag ggcatcgcta ttatggtggg tgtgaatttg tcgatatcgt
    12241 tgaaaattta gcgattgacc gtgcaaaaga attattcgga gcgaaatttg cgaacgtcca
    12301 gcctcactcc ggctctcaag ccaacacggc tgcttatctc gctttggtag aaccagggga
    12361 cacgatctta ggaatggatc tttccgcagg cggacatcta acacatggat caccagtcaa
    12421 cttcagtggc aaaacctatc actttgttgc ttacggtgtt gatccgacaa cagaagttat
    12481 tgattataat gtggtacgta ttttagctag aaaacatcaa ccaaaattga tcgtagcagg
    12541 tgccagtgct tacggacgaa cgatcgactt tgcgaaattc agagaaatcg cagatgaagt
    12601 aggcgcaaaa ctgatggtag atatggcaca tatcgcagga ttagtagcag caggacttca
    12661 tcctaatcca gttccttatg cagatatcac aacgactacc actcataaaa cattgcgtgg
    12721 ccctcgaggc ggcatgattt tgacaaacga tgaagcatta gcaaaaaaaa taaatagtgc
    12781 ggtatttcct ggtattcaag gcggccctct agagcatgtg attgcgggta aagcagtagc
    12841 attcaaagaa gcattagatc ctgcatttaa agaatatagt gaacaaatca tcgccaatgc
    12901 aaaagcaatg gtcaaagttt tcaatcaagc aattggaacc agagtcattt caggggcgac
    12961 agataatcat ctgatgttga tcgatgttcg cgagttaggt ataaatggaa aagaagcaga
    13021 gagcattttg gacagtgtga atatcactgt aaataaaaac tcgattcctt ttgaaacact
    13081 aagtccattc aaaacaagcg gtattcgaat cggcacgcca gcaatcacta ctcgcgggtt
    13141 caaagaagaa gatgctgtaa aagtggctga actagttgtc aaagcattac aagcaaaaga
    13201 tgataacgct caactagacg aagtcaaaac aggtgtacgc gaattaactg aaaaatttcc
    13261 gttgcataaa aaataaaaat ttaaattcca ttagtaaaca gaaaagttca atgttgggct
    13321 tttctgttta ttttttggga aaacggattc tttgacaata tcggtgatgc tctctagtat
    13381 atgaggtgta attttagtct tcacactaaa tctcagggag gaaaagtcat gactgatcgt
    13441 atctcgttag aaaaggaagc agtagcattc agtgatgcaa atgcgcctca tccgcgtatc
    13501 tatgaactag caccggaaga aggaagagtt ttactagaag aagtacaaga atcacctgtg
    13561 aacaaatatt ccgtcgatat tgaagacttg atggtggata ctggcgaatg gggaaaactc
    13621 aatattcgtc tcgtacgacc agaagggaat atggataaat taccagttat tttttatatt
    13681 catggagcag gctgggtttt tggcagtgct caaacccatg ataaattgat cagagaactt
    13741 gcggttcgta cgaattccgt cgttgtcttt ccagaatata gtcgatcacc agaagcaaaa
    13801 tatccaacag caattgaaca aaattatgct gtgttgcaac agctgaataa attagccaag
    13861 tcaaaaaatc tggatctcga gaatctaatc gttgcaggag attcagtagg tggtaacatg
    13921 gctacagtca tgacgatctt aacaaaagaa cgaaagggcc tcccaatatc caagcaattg
    13981 ctgtattatc cagtcacaaa cgctgatttt gatacagatt cctatcatga attcggagaa
    14041 aactattttt tgacaagaga aggaatgatg tggttttggg accaatatac gacgaatgaa
    14101 gaagagcgtg ctgaaatcac agcttcgcct ttgagagcaa atataaatga actaaatgat
    14161 cttccttcag caatgatttt aacgggagaa gcggatgtat taagagatga aggtgaggct
    14221 tatgcgcgca aacttcgtga tgcaggggtt cctgttacac aagtacgctt ccaaggaatc
    14281 atccatgatt ttgtcatggt aaatgctatg gatcagacaa aggcaacgcg tgcagcaatg
    14341 gcattatcta cttcttggat caagaaaaaa gaataagctt tgcttgtccg caggaaaact
    14401 tcttttcaaa aacggatttt cttaggaaaa atggcgagtt tgactagtca atcacttgat
    14461 tttgttttac aattaaaaag aaacgacaga acaaaggaga gcttaaaaaa tgggcaaatt
    14521 tcaagtgatc gatcatccat tgattcaaca caaactaact attattcggg acaaaaactg
    14581 cggaacaaag gtttttcgtg aagtagtcga tgaaatcgct atgttgatgg catatgaagt
    14641 atcacgggat atgccattgg aagacatcgt gatcgaaaca ccgatcactg aaacaacaca
    14701 aaaaacgttg tctggtaaaa aagtagcgat tattccaatc ttgcgcgcag gtattggaat
    14761 ggttgacggt atcttggaat tgattccagc tgctaaagtg ggacatatcg gtctttatcg
    14821 agatgaagaa acattggaac cacatgaata tttcgtcaaa cttccagaag acattgatgc
    14881 acgtcagttg tttgtagtag atccaatgtt ggcaacaggc ggatcagcaa tcatggcaat
    14941 cgatgcattg aaagaacgtg gcgcgtcgaa cattaaattt gtttgtttgg tagcagcacc
    15001 tgagggagta aaagctttgc aagaagcgca tcctgatatt gacatctata cagcttctct
    15061 agatgaaaaa ttgaatgaac atggttatat cgttcctgga ctaggggatg cgggtgaccg
    15121 tttgtttggt acaaaataag taaatacgaa aaagggtgtg acacaacttt tgtcacatcc
    15181 ttttttggtc atttttaacg tttgatgcag aacagctttt tcacagcttt cgtttgcata
    15241 taaaaaaacc atcccaaaaa tgcagagatg gtcaaattca aatctttttt agttctttgt
    15301 agttaacgat tactacaccc ttactttgcg tcatcaagtt cgcattttct gcagtttcat
    15361 caatctcaac aacagcggaa tttttcaatt gctttgaaat cgttccagtt tgcgacttgc
    15421 cgtgaattgt gaacattacg tgagtattga tcttgaattt ttcaccaatt tccgatggta
    15481 cgatagttgt atcaaatttt ccaaaagttg ccattttcat aaaacctgcc tttcacatca
    15541 attataccac gttttatgaa aaatatcatg aaattaaact aattttccat cggactcttt
    15601 acaatcagcc aaatagagta tataatttct tttggataag gccagcttag ctcagttggt
    15661 agagcaacgc actcgtaacg cgtaggtcgt aggttcgatc cctatagctg gcattaaaag
    15721 agaagacaga tatatcaagg atttttaatc cattgataca tctgtctttt tgtttaaagg
    15781 aaataaagta tgctttctta gcaatagagc atgtattatt tctataaaaa tttaggagaa
    15841 taaaacgact agcaatatag ctgtgaaatc aaatatcaca tgaactgccc aacttgtaat
    15901 gatcgagttg cttttaatgg cagcttggtt gaataacaat cttgccaccc cttgtatcaa
    15961 aaggatgtgt gctacagtgt gtaaaatatt tccattatca taagtactgt aatggataaa
    16021 tccaaagatg attgctgaca agaaactagc gacaggaatg ctgaacttac tagaaaaaat
    16081 agcgagaaaa tacactgaga acaattcttc tccaagtatc atgataggca aaataataaa
    16141 taataacggg ctgaaagcct catttattgg attgcctgtt aaaccccatt tcaaaatttg
    16201 ttgcaaaaat accgagactg cggcactgat tactacattg ataaaaaaat atttggctat
    16261 atttttgaca ggagcgacag gtttcgagaa catttttttc gtatcttttt taccaaaaac
    16321 aagtgtaaat gctataaatc cagtgatagt taaagaaaaa atgctaataa tggatggcgc
    16381 aaatagcaac cctaacaaac cgagtacaac gacaatagtg cccaatatcc cttttttaag
    16441 ttctattttt tttcctaaaa tgttattcat gataataact cctactattc agtttttgaa
    16501 gatagagtca gtatacatga agagttaaaa taggcaatgt ttttttatgg atatttgtat
    16561 tgctaaaaat ttatcttata tattcatttt gattatacta cttttatttg taaaaatttt
    16621 ctagcaaaac caagtgcttg caactgatta agcgtacaat tttttagatg ttccgagcgt
    16681 acttcaatca tttcaaagga gttgctactt aaatcgatat tttttaaatc agtatcaaaa
    16741 aagctagcat tttctaaatc attagtagaa aacattattt tagtgaggga acagtctaca
    16801 aaatctgcgc ttgataagac agagtgctca aaggtacttt cttttatgtt tgaaccagat
    16861 aaatttacat agtccatttt acaattctcg aataacgtgt tcccaatata actttccatc
    16921 aatttagtac ctgttaattt acaattgaca aatttcacgc gatgcaacat gctttttgtc
    16981 aatatacagc ctgaaaaatc acagttttcg aatatacagt cggtaatctc cattctttga
    17041 aaatcattgg aagcaaaaaa tgcatacttg aaaatagatt tgtaaataat tggatggcag
    17101 attatttggt tttcgatgac ctctttttgt gaaaggatat tatacagttc aaaatcttcg
    17161 ttttcataga tatgaaaaaa ggatccttta ttcaagcttg cttcattaac tttaggaaaa
    17221 atcatattta attacctcca ttttcataaa gtcatattaa caaaagctaa accaacttac
    17281 attagcagaa aatgagatag gttttcgcct ttttcgataa cgatagtgtc gctatctctt
//
LOCUS       DARDHA010000047        17274 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000047 DARDHA010000000
VERSION     DARDHA010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.047"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            164..1309
                     /locus_tag="SDA10_001272"
     CDS             164..1309
                     /locus_tag="SDA10_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG4240192.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1446..1790
                     /locus_tag="SDA10_001273"
     CDS             1446..1790
                     /locus_tag="SDA10_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEG4240193.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            2061..3347
                     /gene="efmA"
                     /locus_tag="SDA10_001274"
     CDS             2061..3347
                     /gene="efmA"
                     /locus_tag="SDA10_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HEG4240194.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3485..4084)
                     /locus_tag="SDA10_001275"
     CDS             complement(3485..4084)
                     /locus_tag="SDA10_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEG4240195.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4488..5609
                     /gene="mnmA"
                     /locus_tag="SDA10_001276"
     CDS             4488..5609
                     /gene="mnmA"
                     /locus_tag="SDA10_001276"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEG4240196.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5855..7276
                     /gene="arcD"
                     /locus_tag="SDA10_001277"
     CDS             5855..7276
                     /gene="arcD"
                     /locus_tag="SDA10_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEG4240197.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7290..8408
                     /locus_tag="SDA10_001278"
     CDS             7290..8408
                     /locus_tag="SDA10_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEG4240198.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8468..9760)
                     /locus_tag="SDA10_001279"
     CDS             complement(8468..9760)
                     /locus_tag="SDA10_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG4240199.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9892..10470
                     /locus_tag="SDA10_001280"
     CDS             9892..10470
                     /locus_tag="SDA10_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4240200.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10599..10976
                     /locus_tag="SDA10_001281"
     CDS             10599..10976
                     /locus_tag="SDA10_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEG4240201.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10909..12981
                     /locus_tag="SDA10_001282"
     CDS             10909..12981
                     /locus_tag="SDA10_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEG4240202.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(13192..14139)
                     /locus_tag="SDA10_001283"
     CDS             complement(13192..14139)
                     /locus_tag="SDA10_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG4240203.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            14385..14849
                     /locus_tag="SDA10_001284"
     CDS             14385..14849
                     /locus_tag="SDA10_001284"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEG4240204.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(14911..15954)
                     /locus_tag="SDA10_001285"
     CDS             complement(14911..15954)
                     /locus_tag="SDA10_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEG4240205.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(16331..>17274)
                     /locus_tag="SDA10_001286"
     CDS             complement(16331..>17274)
                     /locus_tag="SDA10_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEG4240206.1"
                     /translation="PIPICGLILGLASLGNLFKDYHHVALGNLTGGISMILMIFIFVK
                     LFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWLCAVIFQVI
                     LVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFFWISLICYA
                     FLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLVVALFALSQ
                     VLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYSSVLDFLKV
                     AECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         5700 a         3140 c         3623 g         4811 t
ORIGIN      
        1 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
       61 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
      121 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
      181 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
      241 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
      301 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
      361 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
      421 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
      481 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
      541 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
      601 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
      661 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
      721 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
      781 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
      841 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
      901 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
      961 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     1021 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     1081 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     1141 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttggaagcaa tgtatggaga
     1201 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     1261 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     1321 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     1381 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     1441 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     1501 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     1561 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     1621 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     1681 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     1741 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     1801 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     1861 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     1921 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     1981 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
     2041 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
     2101 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
     2161 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
     2221 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
     2281 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
     2341 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
     2401 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
     2461 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
     2521 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
     2581 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
     2641 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
     2701 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
     2761 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
     2821 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
     2881 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
     2941 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
     3001 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
     3061 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
     3121 gtgtcttagg gaacttcaac tctttgatga acctgacagg tccaattgga ttattatttg
     3181 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
     3241 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
     3301 tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt
     3361 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
     3421 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
     3481 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
     3541 ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc
     3601 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
     3661 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
     3721 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
     3781 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
     3841 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
     3901 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
     3961 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
     4021 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat
     4081 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
     4141 cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca
     4201 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
     4261 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
     4321 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
     4381 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
     4441 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
     4501 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
     4561 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
     4621 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
     4681 aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
     4741 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
     4801 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
     4861 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
     4921 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
     4981 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
     5041 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa
     5101 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
     5161 ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
     5221 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
     5281 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
     5341 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
     5401 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
     5461 atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
     5521 cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
     5581 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
     5641 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
     5701 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
     5761 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt
     5821 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag
     5881 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
     5941 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
     6001 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga
     6061 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
     6121 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
     6181 gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat
     6241 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
     6301 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
     6361 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
     6421 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
     6481 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
     6541 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
     6601 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
     6661 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
     6721 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
     6781 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
     6841 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
     6901 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
     6961 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
     7021 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat
     7081 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
     7141 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
     7201 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
     7261 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
     7321 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
     7381 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac
     7441 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
     7501 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
     7561 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
     7621 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
     7681 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
     7741 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
     7801 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
     7861 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
     7921 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
     7981 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
     8041 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
     8101 aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
     8161 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
     8221 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
     8281 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
     8341 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
     8401 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
     8461 tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt
     8521 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
     8581 tgaccaaaac aagaagagaa gtcgaaagaa agacgccacg ataaccaaat gcactggaaa
     8641 cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac
     8701 tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag
     8761 ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat
     8821 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
     8881 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
     8941 caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca
     9001 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
     9061 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
     9121 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
     9181 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
     9241 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
     9301 cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
     9361 gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
     9421 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
     9481 cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga
     9541 taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
     9601 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
     9661 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
     9721 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
     9781 aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc
     9841 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
     9901 aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
     9961 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat
    10021 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    10081 acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt
    10141 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    10201 gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga
    10261 atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa
    10321 gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt
    10381 tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg
    10441 atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag
    10501 atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    10561 aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    10621 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag
    10681 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    10741 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    10801 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt
    10861 tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta
    10921 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    10981 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    11041 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    11101 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    11161 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    11221 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    11281 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    11341 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    11401 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    11461 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    11521 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    11581 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    11641 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    11701 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    11761 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    11821 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    11881 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    11941 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    12001 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat
    12061 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    12121 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    12181 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg
    12241 agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca
    12301 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    12361 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa
    12421 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    12481 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    12541 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    12601 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    12661 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    12721 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    12781 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    12841 gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    12901 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    12961 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc
    13021 aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat
    13081 ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt
    13141 ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg
    13201 aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg
    13261 tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg
    13321 gattccacca atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc
    13381 aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa
    13441 agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat
    13501 cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt
    13561 ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg
    13621 gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc
    13681 acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt
    13741 cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca
    13801 atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg
    13861 cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag
    13921 agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga
    13981 ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata
    14041 ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt
    14101 ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt
    14161 atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt
    14221 gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt ttaccatgtt
    14281 tcctcggttt atcaaacttt taagaacatt ttttattagt tggcaagtag ttttggatag
    14341 agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa atacacacac
    14401 ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta tcttttcagc
    14461 caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa gaacagtttg
    14521 aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata aagagaggta
    14581 cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga gaaatccgaa
    14641 cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg attgggcagg
    14701 caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg
    14761 gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt
    14821 tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat tgttccatgc
    14881 gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa taaatagtaa
    14941 agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc gtgtaccttc
    15001 tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag tgatctcaaa
    15061 ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa acaagctgtt
    15121 gaccaaacaa aagatcacaa accctaatac ataccaagga agcggtaatt ttcgtttttt
    15181 ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga atttttcaaa
    15241 cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca tggcgaattc
    15301 gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag attggagtgt
    15361 accacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta atccaagaaa
    15421 agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga tctgtccttt
    15481 ttcttcctct ttcgcatgga tggaaggggc gattgaagca atagcagaag aaccacaaac
    15541 agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt ttttaccaat
    15601 caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa ttccttttac
    15661 accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa cagagaattc
    15721 taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt taaaaaaagt
    15781 atttcctaat aaaataccca ataaaatagc aatagtcgct ccacctaact gtggcagata
    15841 aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat
    15901 ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt tcatttgatc
    15961 ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact aaaccagtat
    16021 tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt ttcgaataaa
    16081 cggtgttcca gaagtagctt ctacggaaat aagccgaaat ttcacaaaaa tttgagaagc
    16141 aattttcgta aattccttct tatttcttga agctgaccac ttcttttacg acctcttggt
    16201 aaacggtgtt caaaagcttg catctgcggt aataagccca accagtacaa aaacatgaac
    16261 agctgttttt gtttggccgt tcttattact tgatgcagaa cagctttttc acaacctatt
    16321 gaatcgatta ttaagcgatt tttttagaaa caaattttcc atgtttgcta tgttctttaa
    16381 ttaagaaacg tacgtaatga gcaagaacgt agaagacaac tacacttgca aacagacatt
    16441 ccgcaacttt taagaaatcc aatacgctac tatagatacc taaactgttg aagtaatgaa
    16501 cagtagtaaa tatagctgtt gcagagataa caagcgggaa tgtaaaggct gcatagcttg
    16561 gataaaaacg aagcttgaac aaacgtggaa gcatagacag cacaacgaaa tacaatactt
    16621 gagataaagc gaacaatgct acgacaagat aaaaattagg ttcagcaaag tttttaagat
    16681 aacctgttag gcaaagagaa ccaggtgctg caacgatcgt aatcagcgga cgtgtcggat
    16741 gagccatatt tcttacgaaa aagacacgtt tgatgatgat cggcaataag aacgcatagc
    16801 agatcaatga aatccaaaag aaaatttttc caataagtgg gaagaagttc ccggcagtta
    16861 cagtgatgac cccaaatcca acgtaaatga taaaccagct tggataaatt gcttcaattg
    16921 aaagatcagc tttgataaca tggtgataat tgaaataaaa gacaaggatc acttggaaga
    16981 taacagcgca cagccaaatg aatttgacaa ttggtgcaat actttcatat gaaacaaaat
    17041 aagtacaaat aaccatgagt gacatcgtaa aagttggtga aacggaggcg atgattggat
    17101 catgcaacgt ttgtttcgtg tgttcgaata agataaacaa tttaacaaaa atgaatatca
    17161 taagaatcat agagataccg ccagttagat tgcctaaagc aacatggtga tagtctttaa
    17221 acagatttcc aagggaagct aacccaagga tcaacccgca gatcgggatc ggaa
//
LOCUS       DARDHA010000048        16854 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000048 DARDHA010000000
VERSION     DARDHA010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.048"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..1630)
                     /locus_tag="SDA10_001287"
                     /pseudo
     CDS             complement(1..1630)
                     /locus_tag="SDA10_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2185..2748)
                     /locus_tag="SDA10_001288"
     CDS             complement(2185..2748)
                     /locus_tag="SDA10_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG4240207.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(2763..3806)
                     /locus_tag="SDA10_001289"
     CDS             complement(2763..3806)
                     /locus_tag="SDA10_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240208.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(3939..4343)
                     /locus_tag="SDA10_001290"
     CDS             complement(3939..4343)
                     /locus_tag="SDA10_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG4240209.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(4576..5211)
                     /locus_tag="SDA10_001291"
     CDS             complement(4576..5211)
                     /locus_tag="SDA10_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEG4240210.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(5477..6529)
                     /locus_tag="SDA10_001292"
     CDS             complement(5477..6529)
                     /locus_tag="SDA10_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4240211.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(6542..7480)
                     /locus_tag="SDA10_001293"
     CDS             complement(6542..7480)
                     /locus_tag="SDA10_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG4240212.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(7931..8581)
                     /locus_tag="SDA10_001294"
     CDS             complement(7931..8581)
                     /locus_tag="SDA10_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240213.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(8681..9190)
                     /locus_tag="SDA10_001295"
     CDS             complement(8681..9190)
                     /locus_tag="SDA10_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HEG4240214.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(9391..10260)
                     /locus_tag="SDA10_001296"
     CDS             complement(9391..10260)
                     /locus_tag="SDA10_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG4240215.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(10280..11401)
                     /locus_tag="SDA10_001297"
     CDS             complement(10280..11401)
                     /locus_tag="SDA10_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEG4240216.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(11403..11798)
                     /locus_tag="SDA10_001298"
     CDS             complement(11403..11798)
                     /locus_tag="SDA10_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG4240217.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(11817..12623)
                     /locus_tag="SDA10_001299"
     CDS             complement(11817..12623)
                     /locus_tag="SDA10_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG4240218.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(12616..13404)
                     /locus_tag="SDA10_001300"
     CDS             complement(12616..13404)
                     /locus_tag="SDA10_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4240219.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(13418..13894)
                     /locus_tag="SDA10_001301"
     CDS             complement(13418..13894)
                     /locus_tag="SDA10_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4240220.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(13966..16623)
                     /locus_tag="SDA10_001302"
     CDS             complement(13966..16623)
                     /locus_tag="SDA10_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG4240221.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(16642..>16854)
                     /locus_tag="SDA10_001303"
     CDS             complement(16642..>16854)
                     /locus_tag="SDA10_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HEG4240222.1"
                     /translation="MLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLADQAKQA
                     TFQLNDLNKAFLASHIYGKILSAKFE"
BASE COUNT         5342 a         3440 c         2502 g         5570 t
ORIGIN      
        1 cattattttt atatgttgta acttcctgat aagaaaacta attaaaagaa agaggtaact
       61 acataaacta attaaaaaag ctaacttcat agttggagga taaatggaag aaacttcaat
      121 gattgtttct ccttgtgttc cttcaatcga cactagtcct attatcaatc tctctttttt
      181 gtccatttac ttttgcgact gttccccaat aatcaaatac aggaagcgtt attttttgtc
      241 ctttagattc atgtactgta tagactattt tcgaatccat cacttgataa ggcatagtat
      301 gccattcctt gccaattctt acttcatgat tctgcaaatg ttctttgtat tgtaatgctt
      361 ccgtgggtgc atagtcataa agtcctccgg gaaggagtgt cgtgtaacga ataagatttt
      421 cttgattcat taccaagtga tctctctgag tgacaacttt atgttcatag tttgcttttg
      481 ttgcagcaga tacagccaac agtaataaaa caattgccgt tatattaact acttttcttt
      541 gcttagctga tgtgctcttc atgaaaacga acgataaaat caacgagcca aacagtactt
      601 gaaagcctaa gattcgccaa ggaaattgaa tcatttgaat gggtgtattt tgtaataaag
      661 accatggaaa aaatttggtg gataagagcc aagagactac cgcactaagg taaatcactt
      721 tttctttgcg attaaatcct ttaaaaaaga agagtggaca gatcaaagaa ataataccaa
      781 tgattcctac cgtaaaggtt gtcaggtcat tattcatcgc accaatcaaa ctatccgaga
      841 catttaatgc ttggtgttgt aaggtcgttt tatctggctc attaatcgat tgaaccatga
      901 tttgtcggaa catcggatac caaaagtatg ctgttaaaag aatcgtcatt cctactgcct
      961 ttgacaactc cacaatcaac cggatgcttt ctttttttct tgtttcactt actacaaagc
     1021 cgcctaagac tagtaagcca acggctatta cactcataaa agtggaaaga acatgtgtat
     1081 aaatgagtaa gctcatactg actgctaatg gaattgcaca tggctttttc tcataaatca
     1141 gtgcgtaagc ataatacata actatcggta taaccgtaag tgcaatcgct tcagcaatag
     1201 cagaacgatg ataaatatca atcgtacggt acccataaaa tgtatataaa cagctaaata
     1261 taaaagccgc atcgattctt tttaaaaatt ttagtccata atgaaacatc agcatgatag
     1321 tacaaacatt caatagccaa acatataaga tataactaac gattaaatta ttacttatcc
     1381 aataaaaaat gactgccgga aaaaaggtca agtagggata aaaataattc actccgctcc
     1441 cataatgatt aaacgtcgta aaattgattg gaccagaaaa aattgaagat aaccctttga
     1501 ctctagcaaa atggaaggca atatcccccg atgtagaaat agtatgaaca tttctaaaat
     1561 aattgattga ataaagtcca gcaataattg ctaggagaat ggacaatatt atttttttct
     1621 ttacttgcat tacttaatta tatactcctt tcatggattg ctttatctag tataacttaa
     1681 attttaaata aatcgttatt tatttttact ctcacttaaa aaagcactaa aattataaga
     1741 gagggaggta atggtctgaa caccaagaat tgttccaaca tcgaattgct taactaatga
     1801 gttcaaagct taattatgtg gctcttatat ttaccgagcg tggattgcga tctctttagc
     1861 gaaacaaaca tctttcataa aaaaaacagc catatccgct ttcttggata tgactattta
     1921 ctactacttt actatctttc attcttcttg tctcacttat gataaggcgc tgcgtatcat
     1981 gagtgaatga tattaggaaa acttcataaa ataaatcttt tatacatttc cacttctaga
     2041 tggtcattca tgttagaaga atcaaactat ttttttactg tcgtaataat attaaaacac
     2101 tagaacaaat caattaaatt ccataaaata taaataatag acctagcatc atctggtata
     2161 aaatggatgc taggtccgtt atatttactc aaaattgatt ctacgaatag atcttcttat
     2221 catttctatt ttagtagtca tgcgttgttg ttcttctaaa ggtggtaatg gtataagtag
     2281 tttagataaa tttgtaccat ttacatttgg ttgcccagtt ccgtaagaaa ttttttctaa
     2341 taacttccaa tataatggcg agtctaagaa gcagtctacg tattctgata ataatttctc
     2401 aactaaccta attcgaatca agtaagaagc aaaaacagat tcctcactta tttccttaac
     2461 caagaaactt tttcccatag ttcctcctgt ccttgctatc aaaatgtcat tttcttccaa
     2521 tcttaaatca actagtttag aattactaat atcacagtaa ggcacactgg accaatttac
     2581 acgtccctct tgtatatctg tgattctcaa aaacttaaca ttgccttgtt ttttcgccga
     2641 atctgtatag ccatattgaa tcttagtaga aatacttcct aaataaaccc atagtcgaac
     2701 atcgctagat gatttttctt gtcgttcttt atgaacaaca gtgatcatct tgagttgtcc
     2761 gactattttt tagttgcagc aatctttttg ggacgcttat ttgcatgaag caacgttgct
     2821 aagtctagta ataaaacatt ggctaacaca ggattggctt tctcgcgata cgctgcggcg
     2881 ccactttcaa acaatactgc tagacgatct tctacaacaa cgatttgttc caaatacggt
     2941 ggaaatttga ttcgttttag gatccggcta ggagagtccc gctcggacac taagagttcc
     3001 gaggtataag gaccataaga acgactggct aagagataat ggccataaaa agccacacct
     3061 tgaatcttat cagatgtttt tctgctcatt gtcacggttg gttggatttt gtctttgcgg
     3121 cgaattgctg ttttcatttc agctatcgtc ggcaatagat agaaagtcaa ctgtccttca
     3181 ccttttagtg tgaagttgcc agataccagt tgatcattgt tttgagtcaa atacgatgct
     3241 tgtggaatct ctgagagatc agttgtcagc caataagcga ttggctgtcc cgtctcttca
     3301 gatgtatctg ctaagatatc gatcaaacgt aaagcggcta ctcgtccagt agcagatcct
     3361 gtgattgtca accataaaag ttcccgtttt gtatcatata ctaagccccc tacatgtggc
     3421 cgtttaggta aaatcagcag tttgatcggc tgtcctgttt ctctatccag tacatgaata
     3481 atagaatgat gctgatggtc atagcagtag gcagaaataa acacaaaatc ttgagaaaaa
     3541 gccaaccctt gcggcgtcat ccattgacaa acctcatttt cgccggtttc cggcaccacc
     3601 attcttgttt ctgttaatcc aggaatgtta tattgttttg cttcagcctt ttcccaagct
     3661 gcttgaacaa taggataccg ttttaacttt ttttcaatat cctgatgtat ccgttgctgc
     3721 ttccaatgct taaactttag gacatagtaa acaagaaggc caatgaccag caacactaaa
     3781 atcattattg ctacccgctg tgtcattttt ttgcctcctt gttctattcg atcaatcgtc
     3841 catataccgc tattaattac cgtaactaca tgtttctttt actagcgttt ctttatagga
     3901 tctttcataa tctcatttat agtaacatta agaatccctt attctactat agtcacttcc
     3961 acatcaatat tgcctcttgt tgctttagag tatgggcaaa atgcgtgggc ttcttctgcg
     4021 attttctctg cttggtcctt ctcaagtcct ggcaaagaaa cttctaaaac gaccccgatt
     4081 tggtagcccc catcttctaa ttgaaataaa gatacccgtg ctttgacttc agaatcagac
     4141 tcaagattat tttcttccag catgtgttga accgcgccgt tgaagcaaga tgcgtatcca
     4201 gccgcaaaaa gttgttccgg atttgtccct tcactatgga ttcctgggcg gtcgatcttc
     4261 atgtgcatac ttccgtttgg tgcctcaact tcgccatcac ggcctccacg attgatcatt
     4321 gctgtttcgt acattttttt cattgagatc atcctttctt tcgtcgttct atattaagtg
     4381 taacgagtat tttttaatct gacaaaggta atgcttgcat tcggcgacat cagttagaca
     4441 acatcgatga tcagtgatag aagcagttta tctatacaaa aaaagaaggg ttctatgata
     4501 tttggtgtcc ttactcgcct tgagtaacaa caccaaatat catagaacca cttaatcagc
     4561 ttcacactgc tttttctact ttcgtagttg aaaaaaactt aaacaaatca tcggcaaata
     4621 gttaaagaaa aaacttaatc gttgaaagaa gcccaattgc ttaaagaagg caaactgact
     4681 aatcgacgga atccgtgcta atccgtaaag acctgcaatt agtaaactaa taactgttaa
     4741 aacaagataa aaatggctaa gcgtaccttg accactttga cgataaagca gaacaagtaa
     4801 caatgggaaa agtagaaagc ctgcatagcc tagcccagaa cctatattat ggatccaagt
     4861 cgatagatta caagagcttt cattcgtatt gatactgaat aagccagtaa aaatacaatc
     4921 acctatccca tataaaccaa ctgttgcaac aacaagtatt gcaagtgttt ttgatgttcc
     4981 aacaaatagg tgatacaacg caggcaacga aagaacaaaa aaaagtccag atacaactga
     5041 ccagaccaag aaagcacgtc tgactggact atctacgtca ccaaacacac taattacggt
     5101 agtcatttga ttcagtttgg gataaaaaag gccaaggata tacggtgtcg ataaatcact
     5161 catcacacct aataataaaa aataaaaacc atatttttgc aaaaaaatca ttttcttccc
     5221 ctacttttta gtgattattc gatacatcca acaatcaaat tttttctcat tttaacacat
     5281 atttttattc tagttgtctt ttgaacaatc aataacataa aggtgagttt cttctacatt
     5341 ttaaaaaatc gaatggcact gaagagttca gttgtcagca tcaatctcct actcaaaaag
     5401 gcaaaaaaac gtaagtcaga tgttgttgac atctcactta cgcatccatt gattgaaaat
     5461 ttcttagtaa aatctactag cctaaaaatt tgaagacttc ttctcgatca tacagtttaa
     5521 atactttctc gcataaaaat tgagtaatta gtgccgcaaa aacagggatg actaaccaac
     5581 aaagaacaac aagaaagcca tttagccctg catctaaaga agccaacgga ccaacaagtc
     5641 cgaccacgcc gaaaccagca gaagcaggcg tacctgaaat agaaaataga gcgacaggaa
     5701 tagctgtaat cactgcacta actaaacaag gaataagaat cacaggtttt ctaaatagat
     5761 taggcatcat catcttcata gcgcctaaag caatcgcaat tgtcacacct gacttattaa
     5821 ccttccaaga gttcactact aaaacaaaag tggttgcagc tacgcccatc gcagccgcac
     5881 cagcagaaac accatctagt tggattgcca ttccgattgc tactgttgaa ataggagaga
     5941 taatcaaaaa ggcaaaggaa caaccaatca agatagacat taacagcggt tgtaaggtag
     6001 tgaagttatt aatcaatgat ccaattgcag aagtaattgc tgttacaaaa gggaataaat
     6061 aaaaaccaag taaacctgca cctacaccta caacaatcgg tgttaacaca atcgacactg
     6121 acccaaaact atttccaatc agtaaaatca gcccgacagc aatcgatgct gtcagcatcg
     6181 tattaataat atcgcctgta ccagcaccaa cgtaaacacc cgtttcaccc aaatcaggtg
     6241 tgaatttgac tacaccagaa ccaacaaaag aagctccggc tacaaccatc attctcatcg
     6301 gatcaaatcc aaattgtaag gcaatcaaac caccaatgat caaaggtgtt gctagttgaa
     6361 aaataactgc taactgtgcg atcgttaccg caaagggata cgcactaaaa tattttaaaa
     6421 tggcacctaa tactgcatta ggaatcaatc caataatgat tcctgttgct gtaccagcga
     6481 ggactttatt cagaaaaatt ttcggtgata ttttttgtga agtttccatt caatctctcc
     6541 tttattttac gttcaaacta tcttcttttg catggacaag ctcagtcaag aaatcacaat
     6601 aaggcgtcgc aacaccatat ttcttccctt ttctagaaat tgcgccattg atataatcga
     6661 tttctgttaa tcgatggttc ttaatcaagt cttgatacat tgaaggataa tgtaacccaa
     6721 ttgtttccgg gtcaaaacaa gattcacaat gtgcaatgac ttctgggaca tctagttcaa
     6781 tcttctctac tgctgctact ttggcaaact cattgacaat cgtcgccacc attttatgag
     6841 cagtcgatgt ttttcctagc tcagccatat taacgtctaa gattgtgcat agtccgttca
     6901 ttgttccgtt cacacatgct ttgcgataaa tagaatagtg aatattgtca gaaaaatgag
     6961 catttaatcc tgattcagac aatttgtctg ctaatttttt tgcagctgct tcttttccat
     7021 cacctaaatt ttgtaattct actgaaccac ttccaaataa tttgacctga ccaggacctt
     7081 caagacctgc cgtccacatg gtatttccaa tatagatatt ttccattggt acaaatttct
     7141 caataatatc ttcatgcccg ataccattta atagacataa gacctctgta tcttttttga
     7201 ttaatgattg gatatcctgc agcatttttt ccagctgcat cgctttggta aatagaataa
     7261 tcagatcaac ttgatcttct ttttctactt cggattgaag aacgattggt agttttgctt
     7321 ctacctcttt accattaaaa tttgcttgta atccatgttc tttgatttgt tggacatgtt
     7381 ctgcccagcc atcgattaat aatacttcgt tcccactttg gtgaagcatc aacccaaagc
     7441 gacttcccat tgcgccagcg cctgcaattg ctattttcat tgattcttcc tcctttttct
     7501 ttctattata ctcatttgag aatcttttca caacctcgtt ttgttattct tctcttattt
     7561 atcatgttta tatccaccat tcaagttatt ttatcagcaa atcaataaaa actggaatag
     7621 aatcccaatt tcatcgtaag agaaactaga ctagcaataa gaaaatagaa acattcatag
     7681 aaaaaatctc actcctctga ttataaaaga gggggcatta acctcgtgta tactaaaacc
     7741 ttccattcat tcgatggatg attttctttt tgtgaaaaaa tattcattgt agtacgatgt
     7801 tgcgcaacac agacggatgg tattgcttat tatttattaa aatcgaacaa tacacttatt
     7861 taaaaacttc tttcccagat tttcttccat ttttacagtt ataactagta ttatcatgaa
     7921 aaatagcctt ttattgtcta tgggaataat aatttaaaat tggtactttt ttattaatta
     7981 gttcatcgcc caataatctc tccagtttct tagcatctcc tgactcaacc ggaattatcc
     8041 aagcccagtc taaataattt tttaaaatct catcatattt tgcaaatatt tcattgccat
     8101 attccgtcct taaaattata ttttcagtgc cagacaagtg atttatattg agttgataaa
     8161 aaagatctcc atctaaaaaa atatcattag aagaagattt ccccacagta atgaccattc
     8221 catcagctct atgtgttaca tacaaataat tgtatgacct catttcacct aatgaatcaa
     8281 ttatttcttg ccctttagct ctaatatatt gatctctatt acctatctca ctcttattta
     8341 acaaaagttt tttcatgtca aatttaatcg gctgttttat ttttgtaaat tcgcttctac
     8401 tactaatcca atccgagatt aatttttcaa catgtcccag agtaaactct gatttattcg
     8461 ctaaatactt tttttgttgt attaaagaca tctttccccg aagctcaaaa ttgagcctac
     8521 atcgattctc tatctcttca aaaagcatct tatcttcatc attcttatat tcatcattca
     8581 taaaaccaat ccagctcctc gattaagata atattttaat ctcatacaaa aactagctct
     8641 catcaaaaca accattgttt tgatttctca tgaaatttct ttatactttt ctccgattat
     8701 aaattcctaa ccccttgaga aaattacgca tatatttatc tccgcaaggc ttaaaatttc
     8761 gttggccttc ttttctcaaa aatgccccga tttcgccttt tgatggatag atttctgcct
     8821 cagcaaaaac gtctaaaata tcatcagtgg tcatagacaa ggcgattttt aattttttta
     8881 gcaacacatt gttgatatat ttgatttcct tgcttttttg cggcgtgtct actatcggct
     8941 tatctttttg cggaccacgc gccaagagaa tcaggccatt taggaatgcg tcaaaaactt
     9001 ggttgttgat ggttttttca tacacattat tttctgcatt atctttgttc ttatcctgta
     9061 ctttggtcag taccgcctga gcttcttctt tagtcagtgg tacaccgcct agctcaaaag
     9121 ctttaatcaa atccgtatct ttcaaatcaa tcgcataacg caatcgcaat aacctatcat
     9181 tgttattcat ttgttcctcc gaagtattta gtctttactt tcttacgtac tttttgtctt
     9241 gctaagattt ttaggatcaa tgataaaaac cgcatcactt atgataaggc gctgggtatc
     9301 atgagtgaat gatattgctg aatacaaata gtccgaccac attaagttga tcggacttca
     9361 taagcatttt attgactaat acttttttgc ttaagaattg attctaacat cttttgctaa
     9421 agttaagcat cctatagttc ctgcattatc tgctaactta ggtgtgacaa tgtattcttc
     9481 gatatttggc gtatcgacat agccgttaat tagttctaaa aaacgtcttt gtactttctt
     9541 tttcagatgt tcttgttgca tgacaccacc accaaaaatt ataatatctg gtgaaagcat
     9601 cagggtcgta ttacaggcac attgtgcaat ataatccgcc tcgatttccc agaacggatc
     9661 ttctatagaa agatcttgtc ccttggttcc cgtacgcatc tcgatagcgg gtccagatgc
     9721 caacccttcg agacaatcat gatgaaaagg acaagtacca gagaaagtat ctcctgaatg
     9781 tctcttaact aacgtatgcc ccatttctgg atggctaaac ccttcaacaa aaatcccatc
     9841 ttgtatagct cctccaccaa ttccagttcc aacagtgtaa taaacaacac tggataaacc
     9901 ttttccatga cctgaaacat actccccgta acaagcagca ttcacatcgg ttgtccaggc
     9961 aattggaatt tcgaaatgct tattcaacgt tccaacaaaa tcaaaatttt tccaggcagt
    10021 ttttggagta gaggtgatat gtccataggt ctttgattca cgatggatat caatcggtcc
    10081 aaatgaccct actccaatgc tttctaattg ttctttatag tgattaaaga aatcaattac
    10141 caaactcatt gtttcattcg gtgtagttgt aggaaaactc actctttcta aaacctttaa
    10201 atcatcattg ccaatggcac aaacaaattt agtcccacct gcttctattg agccatacat
    10261 ttcttactcc accgtccttt tatctaggaa ttcgttcttc cctgtctaat agtttttcga
    10321 aaaactcatg tggttccttt tggtaccaat aggcaacaga agaaatatcg tcttgacgct
    10381 cataaagctt gatgtcatca tttcctaggg tttggaaagt aacacgtaaa ttttctttga
    10441 aactaatggg gtcgggtaaa tgccagcggt acaacccatg catcggtaaa gcatcatcgc
    10501 caaaaccatg aactggattt ggattaggtt ttccagcact aaaataatcc cgggtgtgat
    10561 ctcttttaga ttgaaaagga tagcccataa atagcgtatt aaaattttga gcatatgccc
    10621 tttctccaaa gttcttttta tggaaagccc aagcgccgcc aaaataatct tctgctcctg
    10681 tagatgttat tgtagggaaa tcttcatctc catctatata aaacttaaat tctccttccc
    10741 cccaccagta tctttccaat gcgcacaaag caaagtaggt tcctacataa tacccagtgc
    10801 ctttgattcc atcaataagg gtgtaatctt ttccttttgt cgtgatgggt tcacggttcc
    10861 atttagcatg aaaatacatc gcatcatccg gtacttcagt caatgcataa ttaaatgtat
    10921 aaaaaatgtg cttaataaac tcaggatgtt cattcgttaa agtaatctta gctttttttc
    10981 gaaatggcat ctcaaaatag caattcatcc ctgatgttgg attcactacg attggtaagg
    11041 aattcacttc gcatcttgtc ccaaacccat tacaaaaaaa atctcctaat ggactttcta
    11101 cagaaggagt ggattcatca tcccaataaa tacgcaaaat taaatcacgt aagacgaaac
    11161 tcccagcgat tgttttgtct gtcactgtca tccaaaaatg acgtaaaatt ccagggcctt
    11221 ccatttctgc taaagtaatt gtctctccag catttatcgg aacagaaggt gacccttttc
    11281 tcccaattcc tagatgactc gctgtcattg ccgctttccc tttttcacca tttttatttt
    11341 ctggtgaaat agttctactt tcctctccct tgtggagcca tatattattt aaacaattca
    11401 ttttattcgt cctctatcct ttcttcactt ttttgattat tcatatagat gatttgttgt
    11461 tgtgcctccg caataattgc tccaatatct aaattatcat ttcctaactg caaagcgatt
    11521 gacattgcca agggaagatt cattcctgca attacgtgca ctctgtcaga aatataggta
    11581 aaaaactttt gatttacact tcctccagcc atgtcagtta aaacaattat ttcgtctgta
    11641 gtatccacta actctttaat ttgttcatct atatcatcaa tttcttcgac atatgcacat
    11701 aagctactaa tttgattatg catacctgtt aaaaactcta gtgtctcttt aaaaccctca
    11761 gctagtattg aatggctagc aatgatcaat cttctcataa ttcaaatcct tttcctttac
    11821 gctaatattc caaatgcagc cataaccatc gcaaaaacaa taacgagtag aatcaaaatg
    11881 gtcatactga tttttttctt actgagtaga taatatgctg cagcagtaat aattactgga
    11941 attaatgttg gtaaaatttt gtccaacatt ggttggattt tcatactaac ttcaccaaaa
    12001 gtgaatttaa gtggcgttga aacactgata actgaagcaa ttaatgatcc aactactgtt
    12061 agccccaata ctgaagcagc ttcagttaaa taattaatct tttcaccgta ttcagtaaca
    12121 atcttcattc cctgcttata accaaattca aagcctctca ttcggaaaaa gaagattacc
    12181 atgattaaaa tgaaccataa aaagattcct acaggattat tttctaaagc catataagcc
    12241 gcaattgatc ccatgatggt tgggatcatc gtcatcaaaa gagagtcccc aataccagca
    12301 attggaccca tcgtaccaac tttaaagtca ttaatcgcat cttttgcttc taatccttgc
    12361 ttttcctcca ttgcaacaca agcacctaaa ataatggctg ccagccaagg ctgtgtattg
    12421 taatactgaa agtgattatt taaagcttcg atgtattcat catcatcttt gtataatttt
    12481 cttaaaatag gtgacaatgc gtaagtcaca gatgcacctt gctgtgtttg ataattaaag
    12541 gtacaaactg acatcaacca acgatagttc gctttcctaa gatccttttt cgttacttga
    12601 tatttttcgc tcattttatt catcctcccc aataaaccca acatcagaag ctcccgcaga
    12661 agcagtgatt tgtgcatctt tttcagctct atgatacaca ttaaatgcta aagcgaatcc
    12721 tatgatcgcc gctccaataa taggtacctt caaaaaagcg gctaagaaaa atccaacaag
    12781 taaataacta atgtatcgac ctgtaggcat aaagcgtaac aacatcgcaa taccaacagc
    12841 aggcaacatt tttccggcta atgttagccc atccataaac caattaggta aaacatccaa
    12901 aacgtaatta acagcagact gtcctaaagt aacggcaata aatactggaa ttgctgcaga
    12961 taaaccaaag aaaattggcc ccaaccacaa aacactattc attttcttaa attctcgttt
    13021 attcgcatat tcctgtgatt tgcgaacaac aaaaccattt aagattttga caattacatc
    13081 tagttgcacc cctaacatac ctacaggtag tcctattgct aatccggctt ctactccaag
    13141 ctggctacca aatgccgttg caataattgc gcacgttcca tagtccggta cagatgagcc
    13201 accaaaaccg gcaactccta atgacatcaa ttgaactgta ccgccaacta tcaaacctgt
    13261 ttttatatca cccatcaaaa ttcctgtgaa taatccccaa aaaactgcgt aatttacaaa
    13321 gatcatcgga atcccataat aatcaacatg tttaataaat gctaatatag tcagcataat
    13381 aatttgaaaa atagtaaaat gcattatcct tactccttta tcctaatttt gattgaattt
    13441 cctgtgcctt atccgccgga acaaattgtg taacaatttc tacaccagaa tccaacaatt
    13501 ttttatacgt gctgatattt tcatctgatg cataggctct agaagatagc tgtaatgcat
    13561 tatctttaac aacagtaccg ccaacaatta gtcggtcaat tctaataccg ttatctacta
    13621 aatgaaggat tgtttccggc tctttcacga tgacaaatac tttttcctga tcgtatttcc
    13681 cctttttaaa attcgcaatc gctgtttctt cagtgataat tgatacagcc attccagcag
    13741 gtctagctaa acgcatactt gatttttgga tgtcgttatt tgcgacagaa tcatcgatca
    13801 ccatcactct gtttgcttta gacattggtg cccattgcgt gacaataatc ccatgcaata
    13861 aacggttatc aactctcgac ataacaatac tcataaatac ttcctccttt atctttcatt
    13921 ttcttacaag caattaccat gccaaaacaa aaagaacttg ataaatcaag ctctttttac
    13981 ataatcataa acgtatttaa cctcatgaat cgggatgtcg acactataat aatgttcaac
    14041 gacactgaaa gattgcttaa aatgtgtctg aaatttttga aattcctcac tcgtttcatt
    14101 gaaataaatt tcatcttcca aagtattttg ttttactaat cgttcaatta agcaactaat
    14161 gtgaacatat aatccgaaac acgtattatt atcaaatgaa attcctagat cattttgtaa
    14221 gatgtatata gcatctgaaa cccgttctaa taatttagtc ggatttaata tggtcaattg
    14281 ctccattaaa ttcgttagac taaaattctt cagaatatta ttgttcaaaa ctttcagttc
    14341 attatcatta aagtgtctat tcaattgatt ggagaattta cttatattca ttcctaaaat
    14401 catatcttca attgaaaaga attctacatc ttctagatgt ggatttagtg ttccaacaat
    14461 aaaaagaata ttgttctctt tacgaagctc cgataaatag ttttcattca ctaaagagta
    14521 atagtcacac gtttctatga caagatcagt atcccttgga aagctatctt caagtatctt
    14581 ttttaattta aacgaagttc ctaatccgct tgcacaagaa cagacaatca tatccttctt
    14641 aatatttgat gattcataaa tataatccac attttgattt tcttttactt gttcaagaat
    14701 ctgtttaagc ggtaaatcta ataatatctg atttgcaaca tcaatagcca ttcttgttga
    14761 aacattgttt agtagtccga attttttttg agcgtctagt tccaaattta gataaatttt
    14821 ctccagtgaa cccatatcaa caagtaaaat aatttcttca tatccggtac actcttgtaa
    14881 gtatctatta attttgtctg caatctcact acttgaagtc attagtggca tatcaatggc
    14941 gtcgtatata tatctctcaa cgatcctatt agcagtactt gctatacagg ttgccgttag
    15001 atctccatga gccaaaatca gtgctaaacg tttttgctga aattctttac tacacattaa
    15061 cttaaataaa ataaccaaaa catttttttg aatttttgtt aaataaatat cccctatttg
    15121 aataaaacga ttgactaatt gaacttctcg actacaccaa ctattaatat cattgatgaa
    15181 ggaatataat ttatcgtccg atgttaattg gatttgataa atccaagaat tcaaaaagat
    15241 agacgttatt tgaagataat aatagtcaaa ttctatttga aaatctctct ctatttcctt
    15301 aacatactgt ctaaatcgaa tctcttgata ggtgggatat gttaaagctg aattattttg
    15361 gatatcaaca ttcttttgtc taaaatatct atcatatttc tcaaaaagct gctgcttatc
    15421 agtgtctttc ttccacgttt gataaagata ctctataagt tcgttgatat taaaaacttc
    15481 gtctactttt tccaaatcat ccatttgaat atattttaat tcattctgtc ttttcataac
    15541 cttcatgccg ttgaaattca cccattgtgg taaatgatac atctgaatat aaagttcttc
    15601 caaatcatct aagaaataaa aggcattggc acaggtcaat tgaatgatat ttttcaaatt
    15661 gccaacattt tcactaaagc gatagtttaa taaaaaatct aataattgtt gagatatcag
    15721 tattttcttt gctagtcttt cttcttctga tttaaaaaat atcttgatta actgttttct
    15781 ttcataaaga gaacgactgt ctaatgttgg aagtgttagc tttacaggta ttcttctcaa
    15841 caaagttgat aatagtttat cctcaggtgg ttctgatgtt gcaaatataa tccagcaact
    15901 tgatgtgtac cagttttcat tatcacccat ccgatgaaat ttcctcgtat ccataaactg
    15961 aaaaagtttc tcctgacatt cattgctcaa tgcatgaatt tcatctaaaa ataagactcc
    16021 attattagct aattctgcta aaccctttct tccatcttcg gctcctgtat atgcaccttt
    16081 tacatgccca aaaaaattgg acaagaaaag ttctggatta ttggcatatt cactacaatt
    16141 aactgaaatg aaagatgcct tctcttctat ataattattg gccaacaaat ccttatacac
    16201 ataatttgct aaatagcttt ttccagttcc agtctcgcca tgaagtaaaa tgggtaagcc
    16261 atttggcgga tagtagattg ctgctttaat ttgagcaatt acatttcgca gacttccttg
    16321 acttccgata accctttcta ttccaaccct aaaagagcta tcataccatt tttccgcttc
    16381 ctctgaagag ctaaaaattt gttgatttgg aactttctcc ggtaaagaaa agaagattac
    16441 tggacgagta ttgatttttc caaattctcc atcttttacg aattcattca aatattgaga
    16501 gacaatatta cgactaacat ttaatttttc agatattttc tgtgctgtat tgttccccca
    16561 tttattttta atctcagagt tctctatgac ataattgaca atgcgttctt ttaattcttc
    16621 catcacagca cctcctcgac actattcgaa ttttgccgac agtatttttc cataaatatg
    16681 acttgctaaa aaggctttgt ttagatcatt caattggaac gttgcttgtt ttgcttgatc
    16741 cgctaagacg ttgaaaagat actctggcga aatcgcatac cccaaaatat caacgattgt
    16801 tgcaatcaga tcttcttttg attcttcatc tgaaagtaat tcacgataca acat
//
LOCUS       DARDHA010000049        16454 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000049 DARDHA010000000
VERSION     DARDHA010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.049"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            17..1189
                     /locus_tag="SDA10_001304"
     CDS             17..1189
                     /locus_tag="SDA10_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG4240223.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            1176..1880
                     /locus_tag="SDA10_001305"
     CDS             1176..1880
                     /locus_tag="SDA10_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEG4240224.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            1885..3045
                     /locus_tag="SDA10_001306"
     CDS             1885..3045
                     /locus_tag="SDA10_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG4240225.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            3121..3399
                     /locus_tag="SDA10_001307"
     CDS             3121..3399
                     /locus_tag="SDA10_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEG4240226.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            3380..3751
                     /locus_tag="SDA10_001308"
     CDS             3380..3751
                     /locus_tag="SDA10_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEG4240227.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            3767..4171
                     /locus_tag="SDA10_001309"
     CDS             3767..4171
                     /locus_tag="SDA10_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240228.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            4168..4578
                     /locus_tag="SDA10_001310"
     CDS             4168..4578
                     /locus_tag="SDA10_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240229.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            4639..5226
                     /locus_tag="SDA10_001311"
     CDS             4639..5226
                     /locus_tag="SDA10_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG4240230.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            5299..5766
                     /locus_tag="SDA10_001312"
     CDS             5299..5766
                     /locus_tag="SDA10_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HEG4240231.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            5812..6120
                     /locus_tag="SDA10_001313"
     CDS             5812..6120
                     /locus_tag="SDA10_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240232.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            6315..13049
                     /locus_tag="SDA10_001314"
     CDS             6315..13049
                     /locus_tag="SDA10_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEG4240233.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            13053..13925
                     /locus_tag="SDA10_001315"
     CDS             13053..13925
                     /locus_tag="SDA10_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HEG4240234.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            13922..14971
                     /locus_tag="SDA10_001316"
     CDS             13922..14971
                     /locus_tag="SDA10_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240235.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            14975..15799
                     /locus_tag="SDA10_001317"
     CDS             14975..15799
                     /locus_tag="SDA10_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240236.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            15817..>16454
                     /locus_tag="SDA10_001318"
     CDS             15817..>16454
                     /locus_tag="SDA10_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEG4240237.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         5882 a         2535 c         3521 g         4516 t
ORIGIN      
        1 aaggtggtga aaacatgtga gcttatttga tatctttaag cagtcaataa gaaatgaaga
       61 gccttctgag tggataccgg attttgtata tggagaggaa gtatctgctc gggcatacct
      121 caaaattatg gcaaaaaata ctgttttaga ttttgttgca agaactatgt caacgttaga
      181 agttaagttt aaaaataaag atgggacatc agactgggat tatattctaa atgttcgacc
      241 taattctgat atgtcagcag ctacattttg gcaaaaattt ttttatcggc tcttagatga
      301 caacgaagtg ttagtaatat ttacagatga taatcaattg ctgattgctg atgattttag
      361 tcgaacggag tatgccgtgt atgatgatgt ttttacaaat gtgatggtaa aaaattttgt
      421 ctttgaacgt tcatggaaca tgtctgacgt catttatatg gaatataata acgaagaatt
      481 agaaaggttt acacgaggtt tatttgaaga ttacgccgcg ttgttcggac ggattctaga
      541 agttgcaatg cgtaacaacc aaattcgtgg gtctgtatcc attgattcaa cgggaacggc
      601 gaatgatgaa aaaggaaaag atggtaagac tagatctcaa agattacaag aatatattga
      661 taggatttgg aactctttta aaactaactc agttgcaatt gttcccaaag tgaaaggctt
      721 tgagtatgag gaatacacca ataaacaagg cgtatcgaat caatcgctag atgaactgaa
      781 taagatgaag tcgtctttaa ttgatgatgt tgccaacgcc ataggagtac ctacggcgct
      841 tatttatggt gaaaaagctg aactggattc aaatattaaa gcttttcgta aattgtgtat
      901 tgctccacta gttaaaaaac ttcaagacga attggtggca aaaataatta gtcgtcaaga
      961 gtacaaaaac ggggaacgta tagaagttaa aaaagttctc caaaaagatc tattagagta
     1021 tgctactcaa attgataaaa taacatctgg tggtgcgttt tatattgacg aagttcgtga
     1081 tgaagttgat tatgatgagt tgccaaataa tgaaggaaaa attcgtattc gtacgaaaaa
     1141 ctatgaagaa actgtgaagg gaggtgagaa cgacaatgcc gaaagctaaa aaagtaccat
     1201 ttcagtttac taacgaaatt cagaatggta agcacattct caccttgagt ggaaatgtac
     1261 agaaaaaata ttggcgtgat gatgatgtca taaacgctaa agatattcgt gactctctag
     1321 atactgtaac cgatgatatt gtaatcaagt tgaacagccc aggcggtgat gtgtttgaag
     1381 ggattgagat ttacaattat ttaaaagatc acccatcaaa tattactgta gaagttacag
     1441 ggcttgccgc aagcgcagca acgtttattg tcgctggtgc caatgatgtg attatgaatg
     1501 ttgggacttc tttgatgatt catgaagctt ctacatttgc ttgggggaat aaacaggaca
     1561 tccaaaaaac gttaaacgca ttggaaacga tcgatgattc tatcttagct atttattcag
     1621 ataagactgg ccaatctgtt gataagttgc gtgaatggat gaatgaagaa aaatggttta
     1681 cagcagatga agctgtcaaa tatggatttg ccaattctgt gaagcgtgaa gaaccacaaa
     1741 atgaacctca aaatattgca gcgatgattc aagatgctgt tgccgttgca atagcgaatt
     1801 taaaggaacc tgttactaat aaaatcgaac aagaatcaaa acaaaaatca ttaatcgcac
     1861 gattgcgaaa aggagaataa agatatgtta aaaattacag ataaaactgc agatgcaaaa
     1921 aaggcattta atgctatttc ttcgaaagaa gatgcaacac ccgaacaaat taatgctgct
     1981 ctagaagaat atgtaaccgc cattgcagaa gatgccggca aacaggtgcg tgctgaatat
     2041 gaagaattga aaaatgtgac ggacaaccgt gtattagagg ctcgtggaat tcctacacta
     2101 acagcagaag aaacaaagtt ttataatgaa gccgtaaaat ctggaggttt cgattctgac
     2161 gtagtttggc cagaaacgat tttggaacgt gtttttgaaa atcttcaaaa cgatcatcct
     2221 attttaggcc tcattaactt tacaccaact gtaggtcgtg tcaaagtaat tagagcacgt
     2281 cgtaaaggtg ttgctgtttt tggaccattg cataaagacc ttgaaggcca actagatgct
     2341 gaatttggtg caactgaatt tgttcaactt gcgttgaccg catttttctt aatttctaat
     2401 gataccttag atttaggacc acgatggatt gaccgtttta tcaatctttc tttaagtgaa
     2461 gcagtacgtg atgtttgggc taaaaaaatt attacaggtt ctggtaaaga tgagccaatt
     2521 ggattgctta aagatctaga tggtgcggtc acaagtggtg tatatccaga taaagctagc
     2581 gcagggacat taacttttgc gaaagagaag attgttcttg aacttgcagg ggtgatgaaa
     2641 aaattatcta aatacacata caaagtagat ggtgacgatg aaggtgaagt gaaataccgt
     2701 tctgttgctg gacgagtgta tctaattgtt aatcctgttg attactggga tatcgtagct
     2761 agagttacat ttgcaaattt aaacaatgtg tatgggacta cgatgccatt tatcgctgtt
     2821 gatcatatca tcgaatctgt agacgtacca gaaagtaaat gtattgcata cgttgataaa
     2881 gagtatgaag ctactcaatc aagagctgaa aaagtctatg tatataaaga aacttttgca
     2941 atgaaacgcg caactttgta tgctgtcgac atgcttggta atggctatcc aacaaataat
     3001 gatgcagcac aagtatatga cttggacttt acagcggggg agtaatgtcc cctgcaattg
     3061 gtgagataac accgacgact gatggggcag tcattaatct ttcgtaaatg gggttagagt
     3121 atgaacactg aagaatttat aaaagaatat aaggcccgat ttcgtatttt ccatacttcc
     3181 gaagatgagg atatcgggaa acagttagag agcgggtttg ccgatataaa atctctaatc
     3241 ggcagttttg atccttccac ttatgagaaa ggaaaagaat tagtttatga acgtactcgg
     3301 tatctacgga acgaatcact agagtacttt tacgacaatt ttcaaacaat gattatggat
     3361 gcatcaattg atctggcagg tgctggatat gccaattaaa tcaaattata agaagcctaa
     3421 aattgtagct ggtgatctaa atacgccagt tacttttttt gagtttcaac ctaataaggg
     3481 tccagaacct ggtgatgaag aaaaaaagga actgtatttt tgtacagcac tagtttacaa
     3541 cccatcaatg aaagatcgtg acattcttgg cacaaaagaa ggcgttacga taaaaatacg
     3601 ggatccccat caatcttata ttcctactaa caaacataaa gtaaggatat tagattatcg
     3661 atatggagat aaagtttggg aaataatcga tgtctcaccg gatttggaag aaaattcaat
     3721 tgtaaagata gtattagaat taattactta aatttggtga taaatgatga gtaacgtaaa
     3781 gtttgaaggc gttcaagaaa caatccaagc tttagaaaaa aagtatggag aaaagaaaac
     3841 taagacatta acaaaaaaag caatcaatgt tggcgctgaa aaagtagaaa aacagttgca
     3901 aacggacatg accgttttta aagacaaagg ttatacgatc gctgaggttg ttcgcaagaa
     3961 cgctacgtat agagattaca aggcagaagc tgaaatcggt tggaatggtc cacatcaacg
     4021 atatagaatc attcatttga atgaatgggg atatacacga aagggcaaac aaattagacc
     4081 gagaggattt ggtgtaataa caaaatctct taagaattcc gagctgttgt actttgatgc
     4141 tgtaggagag gaggttaaaa agagtttatg aaagatatgc ttaatatcat ttacgaggct
     4201 ctttgtttga atgagtatat tcattcaatg acctacaacg agaataccga gcaatatcgt
     4261 attaaatttt atgagcaacc tgaaaccgcc gataaaacag gtccaatgat cacaattcgt
     4321 cctgtagata cacctaacga agcgtaccac ggaagtaaca aagagctttc tgtggagcat
     4381 atgatacaga ttgacgttga atcgaaatat agagcaacat gcaagcagat tcaatatgag
     4441 ataaaaaaag agatgaaaaa actaggcttt ggccaaattt ccggtcaagg gttagatgaa
     4501 tactttactg aaacaaaccg ctttgtagac gcgcgtcgtt acgaaggaaa tacaaaaatt
     4561 tacgataccg aatattagaa attaagacac gaaaataacg tgtctttttt tattacccaa
     4621 aattaggagg aaaattttat gactttagta ggattcaaaa aaatgacaat cggggtcttt
     4681 gacagcacag ggaagattcc agcagcaaat ttatatgtaa ttgaaggtga acaaaacaaa
     4741 ggggcgactg tatctgctga aatcagtggg ctatcaaaag aaccatcaaa agtttacgga
     4801 tcgaacatcg cttactatgt ctctcaaaaa ggaacaggcg atgtttcagc tacttttgga
     4861 ttgttagatt taccaacaga agtaaatgac aaaattcttg gttacaaaac cgatacaaac
     4921 aaaatcagct tcttgggaga agatactgag ccaccatatt gtgcaatttt gatggaatcg
     4981 gaggatttga acggcgatac agcaatgctt gcgatgttca aaggaaaatt cagtcgtgaa
     5041 tctattaatc ttaacactac gacaaatgaa gctttcgaac cagaagcaga agaatatgta
     5101 ttttcagcga ttgccaatga tgttgaaggt gatgctaaag gccaaacagt cgcaaaatat
     5161 gttggagacg aagaagcttc tattactggt ttgaaagaga tggtatttcc tgcagcgggg
     5221 gagtaactag ccccgttgtt ggagcaatca ctcccactat taatggggca acagttgaat
     5281 taagttagga ggataactat gccagataca tttaaaatct ataaaaaaaa tggtactgaa
     5341 tttacaaaag tcgcagaggg tgaaagtcca ctttctatta ctggaattgc agcaaatact
     5401 caagttgcta aaggagatta tcaagctacg cgtgtggtcg gaggtcaaga atcagtaaaa
     5461 gtagatattc ctgcattcac cactcttcca atttcagtta ctggtgtgac gttggataaa
     5521 acgacagctg aagttgaaca aggcggaacc ttaaaattaa ctcctactgt aacaccagca
     5581 aatgcaacag acaaaacagg aagctggagc agttcgaata cagctgtagc tacggttact
     5641 ggaggcaacg ttacagtaaa aacagacgct gaagttggcg gaactacaga agtttcattt
     5701 actactacgg atggcggtaa agtagcaaaa tgtactatta cagttattga aaaagcagcg
     5761 ggctagtctt cggactagct tttttatttg gaaaataatg gaggaatcta aatggcaaaa
     5821 gtaagaattg aattgaaaga agaatcagga aagaaagtct atgaaaacat agacacaaca
     5881 gggaaagatt atcgaaaagc tttagaaaca atcaaaaagc taaatgaaga cggagtaatg
     5941 atttggaatc gtttagacat ttatttggat tttgctgtag gtatcttcag ggatagcaaa
     6001 ttgacagctg aacaaattct tgatggctta ccatctgaga aggtaatgag cacattggac
     6061 gacatcctag gagaagtaat ggggattgaa agtaatccag acccagacgc aaaaaagtaa
     6121 cacccgaaga agcagaagaa atgtacttaa acctatgcag agagttagtg aaagcgggat
     6181 ggtcactatc tgacatagaa gataattcct ttgaaatgat gatgaaaatt gcgtgcactt
     6241 cgcctaagaa agagaaacaa aaagaagttg atcttaaaga cttcttgaaa tccatgtaga
     6301 gaggaggtaa acatatggca aatggaaaac ctttaggcaa tatgattatc aaactagact
     6361 tagacagttc agctttttca aaaggtctag ccggagctaa aaacgctgtt aaccatcaaa
     6421 tgaaagcaat gaaatcacag atgcaagtaa tgaactcttc aggaaattct ctcggtgctt
     6481 tacaagcaaa atacaatggt ttaggcggag ttctacaagc aaatgaaaag caagtagaat
     6541 tgctcactaa ggcttataag gatagttttg actctaacgg aaacgctact gcttcgactg
     6601 ctaaatacgc taatcaattg aatcaagcaa cagctagatc agcaagttac gaatctcaga
     6661 tgaaaacaac tgttggacag attgctcgga tgaaagtgga aacggaaggg tttacgggga
     6721 aactaaaggc taattctgat gtattgatta attctgggaa aaaaattgaa gcctttggta
     6781 aaggagtttc tgggatagga tctgccctaa ctgttggagt aacagctcca atagtggcag
     6841 gcgcaactgc tgttactagt gcagctattt catgggaaag tgcttttgca ggtgtaaaaa
     6901 agacaaatga tgaagttgta gactcgaatg gaaacgtagt ttattcctat agcgacttag
     6961 agaaaggtct gcgtaatctt gccacccaat tacctggtag ccatcaagaa atagcaaatg
     7021 tagccgaagc agccgggcaa ttaggtataa agactgaaaa cgtagttagc ttcactaaaa
     7081 cgatgataga catgggtgaa tcgactaata tgtcggctga aactgcggca acttctttag
     7141 cacgatttgc taatattacc caaatgtctc aaaaagactt tgataaatta ggatcagtca
     7201 ttgttgatct aggtaacaat tttgctacaa ccgaatcaga aatcactgaa atgggattgc
     7261 ggttagcggg agctggtaaa cagattggaa tgagccaagg cgaaattcta ggatttgcta
     7321 ctgctctatc ttctgttggt gtcgaagctg aagcgggtgg gtctgccttt tcgaaggtca
     7381 tgatccagat gcaactagct gtagaaaaag gatttggcgc ttttgatcag ctgaaacaga
     7441 tggcagaaga acaaggtgtt ccgtgggtta atttagtaaa tgctgtgaga gacggtggaa
     7501 aatcgttaaa agcagtatca gagcaaatgg gattcacttc atctgattta aagaaaatgt
     7561 ataaagaagc ggacaattcg aaaagttcgc ttgaaaattt tgcgaatgtg gcaggtgtta
     7621 caagtgaaca attccagaag ttgttcaaga gtgatccttc agaagcaatc atcaagttca
     7681 ttcaagggct gaaagattca gaaaaacatg gtacttcagc aatcaaagtt ctggatgaca
     7741 tggatataaa agaagttcga ctacgcgaca gtttattgag agctgcaaat gctagtgaca
     7801 tattcaaagg agcaattgaa aaaggaaacg gcgcgtggaa acaaaataca gctttaacaa
     7861 atgaagctaa taaaagatac gaaacaactg aatcaaagct aaagatattg aaaaacgaag
     7921 tgattgatac agcaatcgat cttggaggtc catttgtaga cgcgttgcgt gatggtctaa
     7981 atgtatcgaa accattgata aaaaacttag ggaatatggc gaaagctttc tcagctatga
     8041 gtgatgatca acaaagaaac attatcaaat gggttggatt agcagcagca gctggtccag
     8101 cgctgaagct tttaggaact ggtacttcag taataggtaa agttacttca actactggaa
     8161 gattgacgaa aagccttgtt gatttagcag cgaaagcagc agaaaagaag gctatggatg
     8221 cctttgcatc gtcggtaaca acagctggtg ctgcagctgc aaagacagca ggagctggag
     8281 gtattggcgg gttagtccct atgatgggaa aaacagctag tgctgcaagt actgctgcgg
     8341 gcgccagcgg aataggcgca atgacaacgt cactaggttt gttaggtcct gctctacttg
     8401 gtattgttgg cgttggtggc gcactggcag ttggctatgg agcatggaaa ttatttggtg
     8461 aagaagcttg gaattcctcg caaagagtaa agcaatgggg aacagatgtc ggtgaggaga
     8521 tagatagtac tcttgacgga gtacagaaga atattgaagg tgcaaatggt caattcggtc
     8581 ttctcaaaga gggttttact gttgatgatg caaataatat ggcggctaat ttcgcaaccg
     8641 ctggaacatc tttggaaaca tctttaacgg gtagaataaa ggcattagac gatgcactaa
     8701 aagggctacc tcaatcagtt caggatgcca tgaaagaagt agctgaatca gaagaagaaa
     8761 ccttagggca atctctttct aaaataaaag acaacaatga agaaataaaa aaaatcagag
     8821 aaagagccgc aaatcagaac agagacttga ctgtttcaga actacaacag atacaggatc
     8881 taatgaaagg aacttctcaa gcgtatgtgg atacgctcga tgtttctgct aaagacagaa
     8941 aaaagatact atcagccatg aatggtgatg ttgcacaagc tacagaagaa gaagcgaaga
     9001 cgtggttaca atctttagcg aaacaaaaag ctgcaactac cgaaaattat agacaaatgc
     9061 ttgaagaaaa aaaggcggct ttaaaggaaa agggagtaag caaagaattc attgatgcat
     9121 tagaagatga aatgaatgat tacatcaaaa ctacaaatca aggattcgat agccaaatag
     9181 ctgctataac aaataaatat ccacaattga ttaacgaagt gagtttatca aatggtcaat
     9241 tatttagcaa aacggcagct gctatggaca atactggtca gtatacaaaa gtttttctcg
     9301 aaagtaatga aaagatttta gcccaagcga agaaaacatc ggatgaaata gcagataatg
     9361 ccgaaaaaaa tgcagaacaa cttggtatgg tagctgatga ggctacgcat gctggatcaa
     9421 cttggaatgg cttagtttta gacgaaaaaa caggaaaagt aaaaactaat gtacaagaag
     9481 tagtgaatga agcaacgaag gatttcacta aatggaatga attgaaacct gttgttcatg
     9541 atgcgaaact aacaacgaat gcaaaagatg tcatttccat agcggcagta caaaatggct
     9601 attgggatga tatggaatgg aaagaaaaag aagcattatt agaagatgag tgttctaaaa
     9661 atgtatacaa ggctttagaa aatgctggta aatggaatga gttagatatt ccttctaaag
     9721 aagcaatcct aacttcaaat actcctgaag ttatgggtga aactctcatg aatttgggat
     9781 tatgggatac ttacaaactt caagtgaaag atttagattt gaatgcgtat aaagtatacg
     9841 atgctatcga tggatcagaa gaaaaattaa atagttgggc tcaattagat aaccccacaa
     9901 aagaattgtt attagataac tcagattttt ctacaaaact ttttaattcc gaagaaatgt
     9961 ttaaacgttg gaatcagtta tcatctaatg caaagtttct ttcggctaat aatactaaat
    10021 ttacagaggt tcttttatca tctgacagaa tgtggaatca atgggtaaac cttcctgata
    10081 atgagaaaag attacttgca gataacgaag atttagctac cactgtttta tcttcagaag
    10141 aaatatacgg aagatggact caattaccag ataatcaaaa atatatgtta gctgacaata
    10201 ctgacttagc aaacaaaata ttcgcttcag aagaatcatg gaatgcatgg aaagcaattc
    10261 cagacacagt taaaaacttg cttggtaata atattgattt actggctaaa gtgaaagacg
    10321 ggactgtaag tgtagaagaa tataacaaag ttatgccact tcttaaaact ctatatggag
    10381 ataacgttaa tttaaaagag gcaatcagtg gggctaaaag ctctatagag aattacaaca
    10441 aaaatatttt tcctagtgat aaaactgcag taggacacga tgaggcttca caagctgcta
    10501 aagatgcatt tgaagcgttc aacatcttcc aaacaaaaat accagataaa attactaaaa
    10561 ctgtatcagc tgactttata ggtcctatgc cgaaaaatgc gaaaggaaca aacttccatc
    10621 ctggcggagc agctatggta aacgatcaaa aagggcctac ttatgaagag cttataacct
    10681 tgccaagcgg agaaactttt atacctaaag gacgtaatgt cgttttagac ttaccaagag
    10741 gttcgaaagt actgaacgcc actaaaacta aacgtctagt gcctaaatat gctgatggta
    10801 taggaaacat aacgacggtt tcatctacgc taaacttaga tgctttaatt ttagctatta
    10861 acgagctaac gacggtattg agaactcaac tgatggcagg aacgactgat gctaaagata
    10921 ctaaactatc agatgaaaca aaagttgccg atccgattat tccggacagt ctttctgaaa
    10981 aatcagatca gtatctagga attggagctc aatggttaac taatctaatg aatggttgga
    11041 actcagcggt tcctcaatat atgaatagtg aaatggtttt tattacaaac tatctcaatg
    11101 cgctaaaact tcaaaacaat cctaattata cgcaaggagc aacatggaat aagaacttac
    11161 tgaatggatg gaatagttta actgggacct ttattgctac aatcaattca ttttgtaatc
    11221 aagcaatggt cacgcttaga aactacaaca ctcctatgta caacaatggc cgaacgtggc
    11281 agcaaaataa tcttaatggt tggaattcat tgtatggatc attcatagcc cgtgtaaatc
    11341 agcttggtaa cgattcgatt aacaaccttc gttctaaaag tggtggtttt tacaatgcag
    11401 ggacattctt gttgcaatct ttaataaacg gaatgaattc gttaggtaat tctctttcta
    11461 ctacaatgaa caatgtcgct aatacaatgg ttggtggtat aggcaaagga gttaatggcg
    11521 tcatatcggg agttaactat gtgcttaagg aagtcgaatc aagtaagaat atcggaaact
    11581 ggccaattcc acaatacgct aagggaactg atggacatcc tggtggactt gctatgatta
    11641 atgatcaaaa aggcctagtt cacgaagaat atgttcagat gccagatgga cgtagtttta
    11701 ttgccaaagg tagagatctt cttgttaatt tgcctaaagg tgctcaggtc ttaaatgctt
    11761 ctttaacaaa gaaattaaag gaacgtttaa atattccaca ctacgaaaaa ggtattggca
    11821 atttagatat tgttgatttg ttagatgatg aaaaaagaat gttagaattc ttaactagca
    11881 aagttgattt ttcaggcatt aatgaaccat ggcttgatat gactaaatca ggaacgtcat
    11941 taatgtccaa agctgcaaat acaatgcttc aatcaaaatt aagtgaattc ttcactcatg
    12001 gcaattttga tggtgcagta aatgccaatg gtgtttatca atatttagtt gatgtggcac
    12061 agaaagtaat gggtaagttc cccggactta cagtaacttc tggatatcga gcaggagatg
    12121 cctactatca cggaaaacgg caagctatcg acttggctta tccaggtatt tctggagatc
    12181 cgagatatac agcagcagca aattacgctt ttgaaaaatt cccttcaaaa attgcgtatg
    12241 tcattacgaa tggacgtgta cgtgaccgca tgggattatc tggaacaagg tcaagcggac
    12301 aatggacgaa ttggccagat ggcgatcact tcgatcatat tcatttaaac ggttcgatgg
    12361 ggtctggcga tatattcaat ggaggagttg gaggaagtgg tgttgcacgc tggcgttctt
    12421 atgtgtctaa agcattgaaa atgaatggat taccagctac agcagcgtat ataaatgcct
    12481 ggatgtccca aatccaaacg gaatctggtg gtaatgagaa agcaatcggc ggcaatgatg
    12541 ggctcgccga aggcaatgca acaggattac ttcaaacgaa acccggaact tttgcagcta
    12601 acgcttttcc tggtcacgga aatattatga acggatttga taacatgtta gcagcaatca
    12661 attatgctaa gaaacgttac ggagttgcag gtatgttgca agttatcggg aaaggacatg
    12721 gatatgccaa tggcggcttg attaccaaag acggactata tcgagcagga gaaggaaata
    12781 aaccagaaat ggttattcca ttgacaagaa aaacaagagc tatcgaactt atgggtcaag
    12841 cattagcctt cctttctgga agcaataaaa aagaaacgaa aagtgagatt aattctgtag
    12901 ataatagaca tatcctacaa gcgatagaaa aacagtctcg tatgacaaat gatctgctat
    12961 cattattact ctcgtttttt aaaagtaaca atcgatcaga taaagaacta gctttagata
    13021 ttcagaaaat tttaatcagg aggacatagt atttgaggac agtaatatta agaaataaat
    13081 tgaatgaaga aattgatttg tccacagaag attattttgc tactgaaatg tcgaatctag
    13141 gttttgaagt tacaaaagag catattggac aatgggggag tttcagagaa ataggagaga
    13201 gtattgaatt gtctgaattt caatcatcag ttatgatttc tgttcacgga tttagagaac
    13261 aacaattata tggttcatta gttgaatttc tggctaatgg accttttgaa ctagaatttt
    13321 cttttgattc agaaacaatg atcaggaaat gtagtttgaa atcattatct aaaacagaaa
    13381 ttgattccca aagctcattg ctaacagata ctttgaatct atattttact tcaagctggt
    13441 attctattaa gcgtgaaaaa ttaatacaga attcaacttc tctcaataca cgtggaaaag
    13501 tttactcatt tgttcgacca tatgtctaca ccaaaaatgt atgggaaaag aaaggtgtat
    13561 ttaagttcag taataattct gtatatttga caaatagtac agagcgaatg tctccgttaa
    13621 aaataaggat tattggagag tgctcaaatc catattggga agttatccaa aactctaaag
    13681 tagtagcttc agatggctat tttattaata tgaaagacac acaaatatta gaagtgtcta
    13741 gtttgctaga agaccaaacg gcaattttaa aagatgtagg tggagttgaa tcttctgttt
    13801 atcaacaaca agattttact aaatcaaact tcgtccaagc gcctctagga gaattcagcg
    13861 ttgtttttca tgttggacat gcaaatgtag agattgaact ttatgaggag cgtgatctat
    13921 tttgatttta gccattaccc tctttcaccg agatatgaat ttatataacg aacgtttaat
    13981 ttcgactgag ttcgaatttg gaacggatga aattggagaa gaagtaagta attttattac
    14041 agataaatat gtccatgtaa aatcaggaga ttttctacta gcaaaattta ttcctagcgg
    14101 gaagattgct tattttggag tgattagctc gcaagaagac aaaaaaataa attgcaaagg
    14161 attactcagt ttggccgata gcgagatacc gactgtaaaa atatcaggtg ctaattatga
    14221 agagcatatt cgacgactga ttgaatacta cttattgaac gatccaacaa aacaactaaa
    14281 aaatattttg gaggtaaaaa cagaaagcgt tacttctcat tcttatcaag ctgttgacac
    14341 aaataagaga cgattgaatg catatataat aaatgggttc aaaaaatata atgttaaatg
    14401 gtatgtcaaa gagattaaag gtaaaaaaat atatactggt attcgagcag ttgatgattc
    14461 aatttatatt aaagataatt catctgagtt taatgactgg gatgtttttg tgcaatctcc
    14521 tggaccaggg aacgagaaca aattattgat cgtagataaa gcaatgaaag atatagaaaa
    14581 tcccttggta ttgtctacgt ggtatttaga tgacgaaaat aatcttacgc aagattcaac
    14641 gaataagaac attgcattgc ctaccgttaa tttagtaaat atatatgatc aaactcaaga
    14701 agatagacct tcttatgaag aggtagctaa atcggagctt aaaggcaacg cctattcaca
    14761 tgaaattaaa gttaatgttg tgctaaatgc tagaaatatc aatgtagaaa atatcgaaac
    14821 aggaatgtta gcgactattg tttacaaaga gcagacttac aaatctgttc taactgcctg
    14881 gcgaatatcc agcagtaaaa agaatataga attgacattt ggtaatgtcc gaagtcgttt
    14941 tatggattat tttgaagata atgggggata aaaaatggtt agcaatgtgg atggatatca
    15001 atttgaaaac gtgaaagtaa gcgcagaaaa tgatgctaga ctttatcacg ttttatataa
    15061 tcggaaaaat caggttattg atggttacga tcagtctatg aatttatctt caagcggatt
    15121 aacagtaaaa gttgctgcag gagcagcgat tattcaaggt cgtatggtcg ttgttcgaca
    15181 agaagaaagt ataacagttc cagcaaactc aagtggttat atagcattaa cagtggattt
    15241 gacacaagaa gttatacctg gatctattct tccagaatcg gaagaatatg aatggactaa
    15301 taatcaagtc aagctagaat ttataacaaa agttataaaa ggtaatttaa acaatggtga
    15361 taaggtctat aatttaccac tatgctcagt taattctact ggatcaactg tttcaatctc
    15421 aaagatatcg gatagttacg agctgactct ctctaaagga gaaattttgt ggagggggac
    15481 tgcgttaatg catgatactc aaactgtcca accttcaaaa aaaatttggc agacagtttc
    15541 aggttttttg ttagtatggc tcccatacga aaacgggcaa gcaattgagg atagatatgt
    15601 aactacgcct ttttataagg agcgtataac ttttactaat gttttaggag aaattgtttc
    15661 aggatttgat gactatcaca aaaagtggtt tagtaaacga ataaactata attcgaatac
    15721 taatatattt acaggtgcag caagcaatgc aagtggagat aacgcaaata tggtgcttag
    15781 gtatatagtt tctttttagt taggaggtat ggaagagtgg caaatttaga aattaaatta
    15841 tctgcaaata agagacagcc ttatctacgt caccgtgttg ttggtagagt tggtgatggg
    15901 gggctcacaa caatcaatgt acaacttctt gaggaagatg aaattacacc ttttgtaatt
    15961 aatctaaacg gtactttgaa atttgtgggc gaagtttcaa acggtaacta taccgaggga
    16021 gaaccagaaa taatcgattc gactaatggg ttaattagtt acacgttcac taagtcaaat
    16081 ttcagcacga gtcatcaatt caaacaagca tattttgaat atgtagatcc taacggcaaa
    16141 aaagtaactt ttcagaactt catcatagac gtgttagaac gagtagatat taattcggag
    16201 caagcgaaat actatatttc ttcattggaa aaattacaga gtgaaatgca aaccactttc
    16261 aatcagttca ttagtgataa acaggtccaa tacgatcaaa tctactcgaa atataacgaa
    16321 ttagtaagat tgataaatga atcagataag caagtaaatg atcgtattga taaggcaaat
    16381 aatcgtattg atcaaaccaa tcagcaaatc ggcgatctcg gcaagctgaa aaagatgtac
    16441 agtaacagca ttga
//
LOCUS       DARDHA010000050        16408 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000050 DARDHA010000000
VERSION     DARDHA010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.050"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            25..1452
                     /locus_tag="SDA10_001319"
     CDS             25..1452
                     /locus_tag="SDA10_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4240238.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1544..1909)
                     /locus_tag="SDA10_001320"
     CDS             complement(1544..1909)
                     /locus_tag="SDA10_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEG4240239.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(1906..2271)
                     /locus_tag="SDA10_001321"
     CDS             complement(1906..2271)
                     /locus_tag="SDA10_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEG4240240.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2463..2711)
                     /locus_tag="SDA10_001322"
     CDS             complement(2463..2711)
                     /locus_tag="SDA10_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240241.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2710..2838
                     /locus_tag="SDA10_001323"
     CDS             2710..2838
                     /locus_tag="SDA10_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HEG4240242.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2895..4589
                     /locus_tag="SDA10_001324"
     CDS             2895..4589
                     /locus_tag="SDA10_001324"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEG4240243.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4593..4712
                     /locus_tag="SDA10_001325"
                     /pseudo
     CDS             4593..4712
                     /locus_tag="SDA10_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            5240..6373
                     /locus_tag="SDA10_001326"
     CDS             5240..6373
                     /locus_tag="SDA10_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEG4240244.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6404..6724
                     /locus_tag="SDA10_001327"
     CDS             6404..6724
                     /locus_tag="SDA10_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HEG4240245.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6753..7217)
                     /locus_tag="SDA10_001328"
     CDS             complement(6753..7217)
                     /locus_tag="SDA10_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4240246.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7349..8602)
                     /locus_tag="SDA10_001329"
     CDS             complement(7349..8602)
                     /locus_tag="SDA10_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4240247.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8631..8948)
                     /locus_tag="SDA10_001330"
     CDS             complement(8631..8948)
                     /locus_tag="SDA10_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG4240248.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(8974..9294)
                     /locus_tag="SDA10_001331"
     CDS             complement(8974..9294)
                     /locus_tag="SDA10_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4240249.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9758..11173)
                     /locus_tag="SDA10_001332"
     CDS             complement(9758..11173)
                     /locus_tag="SDA10_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG4240250.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11190..12506)
                     /locus_tag="SDA10_001333"
     CDS             complement(11190..12506)
                     /locus_tag="SDA10_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4240251.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12656..13354)
                     /locus_tag="SDA10_001334"
     CDS             complement(12656..13354)
                     /locus_tag="SDA10_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEG4240252.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13643..14599)
                     /locus_tag="SDA10_001335"
     CDS             complement(13643..14599)
                     /locus_tag="SDA10_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG4240253.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14712..15545)
                     /gene="msrA"
                     /locus_tag="SDA10_001336"
     CDS             complement(14712..15545)
                     /gene="msrA"
                     /locus_tag="SDA10_001336"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG4240254.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15720..>16408)
                     /locus_tag="SDA10_001337"
     CDS             complement(15720..>16408)
                     /locus_tag="SDA10_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240255.1"
                     /translation="LATTSLVPWYLLQNTPAKLLQFPWRILSLQIIFSSLILTMIFFK
                     NRRYNKTRELFYLFMTVLLLITLTVSSKKNFNQKIISQPGHIVVNKETVEAYTTKGMD
                     YFLYDYSPIASLENKEHIIKHEAKIGSQWVNLPYKTTGSSIIYRVTNEKSQVITLPVY
                     AYLGTNVEINHQPVKEQVNANGLVQISGVKGVADIKVSTSYTVTTIISYLVTLTAYLC
                     LFFKIVFHKR"
BASE COUNT         5113 a         3298 c         2790 g         5207 t
ORIGIN      
        1 aaaatacaag gcggaggctc tactatgatt gaagaatata ttgagaaaaa tattttaaga
       61 cagctgtttt tatgtgggca attctatgta aataaagaag tcaatctaga aaagctctcc
      121 aatctcctgc atgtttgcaa aaccacttta ctcaatgata tcaataacat aaaaaaagaa
      181 tttgaagaac agatcgctta tacacatcgg gaaaaggacc gttacacttt atatttttca
      241 gaacatatac ctcgatgcaa gatcatgcag caattgtctc aaaattcgtt gtttttaaaa
      301 acttgtctgc tttatttgga agaagatgaa cctgattatc ttcaattaac tgaatgtgaa
      361 ttcatctctg tcagcaaagc ttattcatta aaaaaacaag tattagctta ctttaatgac
      421 tgcgggatcg aaatcgaccg ctactcccct cgattcactg aaatggaaag acgtctgctg
      481 cttcttaatg tgtcgtatcg tttaggcggt tttaattctt gggagctacc tgaatcgttt
      541 tttgaacgtg ccgatcgttt cattgaatcc gtaacggaaa atagtggcag attttatgat
      601 aaagaaaata aagaaatctt atcgattggt tttgctatca gtttcctacg ccagcaagtg
      661 tgtgctgtga caatagattc aaaatttatc gaagaaatca aaaaacgtcc tatttacaat
      721 tatgtcgaat cagcatggga aaacactgat ttccaaacct attataaaaa agaagaattc
      781 gcctttattt tgacactatt caatctttgt aattatggtt tccattctta tcagttgatt
      841 gcggaagact ttcaacagct acaccaagtg tttattgaca atactcccga aataaaagaa
      901 ctagttgcta cctttgaatc tcacttcaat caagaattat tcggaaacca accgttcgaa
      961 cgtgcgctga ttcatctgat gcgttcggct tgggataact atcaattatt catgccagaa
     1021 aaattttatc ttttaaatga ggaacagaca aatttatata aagaagtcca gacaattttt
     1081 tccagctggt ccagccagtt accttatgat ctacggttga atcctaattg catgcgggct
     1141 tttgtgatcg aattgagcgg aattctccgt ctgacaaaag aacatctgac tatctatatc
     1201 gtcacaaatt cggatgttca ttatctgatt tatcgggaag ccttggaagc tgtaactact
     1261 ttcgattttc aagttgcacc aacaatttat tcaagtatat ctgatataaa gaagtatgcg
     1321 cagcagtcct caaaccgggt attatgtgaa cgtacattat atacaccaga tgcggtccaa
     1381 tatgaaaata ttattcctat ttcgatcaat acgatcgatc gagctatcat ttcagctgtg
     1441 caaaacaaat aaggggctgt gacaaaaatc atgtcacagc ctcttatttg ttgaagatgc
     1501 ccatttctgt aacaatctct tgatacacgt caggctcaca acctcactta ttttggacat
     1561 ctaggggtcc gatctcttta tttaaaaaat aagaaatgat ggttcggata actaccaacg
     1621 ccgctagttg gaaaatatcc tgcaaggtag gtttgataat cgattcgatg atcccagctg
     1681 aaacaagaat ttccaaactt agaagtatat agcctcccaa catcttttta atagcattgt
     1741 tctcgcttac tcggtcgctt tgcacttttg tatggacaaa taaatatttc aaaaaatcct
     1801 tgattgatag acaaacaccc caaaccaata aacagatagc aaagatatca agtcccagta
     1861 caagtttttc gaaatatgga agaagaaact gcatcaactc tgtcgtcatc ctacctctcc
     1921 tcttctttgt ttttctttgt cttctatttc ctgattcaag aaataagaaa tgactgttcg
     1981 aatgataata ataagtgcaa gcttcaaaat atctgtccaa gtcggcttga taactgactc
     2041 gatgatatca gcaacgacta gtacttccaa gcttaacaaa atataacttg ctagcagttt
     2101 tcttgtatct gcattacgag tattacgttc tgcatagttt ttcttgacat gagaccaatc
     2161 aaaaagtttg aatagaacaa gaacgatccc gacaattaag ataccgatag aacaaatatt
     2221 taaaatagca atgatccatt ctagaaattc aattaaattt gagcgaaaca tatacgctac
     2281 ctactttctt ttaatttgtt cctattcttt tattgtaaaa gaaagttacc ttgttcgtct
     2341 atttaaaagt tgttctaatg ataacggttc gcttaaattt ttctttttca ctctcccatt
     2401 tttctccctc catccataaa tcgtggaagt atccaaaaca acgtagcaag ttaaaataag
     2461 gcttagtccg tcatcagctt ttcaaagaag cactgtctcg gtgctttttt tgtacccaaa
     2521 tttctaaaaa tagaaccata cacctcctca aatcttttct tcttacctac cctattttta
     2581 tttacaggaa aatcagacac ttcttctttt ttgtcaaaat ttcctttttc ttctcgtcct
     2641 atcatttggc attctaataa aaacgaacta tccttggata tagaaagaga aaacaaggag
     2701 gcgtttttca tgagtaaaaa attaatcaaa gtcggcatcg gtttaggttt gcttgccctt
     2761 ggcgctgcat atctaggaaa aaagacagga ctattcgaag atgacagtca tttatatgat
     2821 gaattcgaat caatctagca tcctatttag ttgaatcact acggacatac ttggaagaca
     2881 ttggaggaat cacaatgaga aaaagtaaag caataatgat tggtgccggt attgccaata
     2941 tggcagcggc tgtttatttg attcaagaag gcaaatggaa aggggatcag atcacctttt
     3001 attcattgga tgatcacggt tcaaatgatg gtgcaccgac cgacactgta gtggatgaat
     3061 attggaataa aaaccatcca ttagaaaacc aaaaaggcta tattgctcga ggcggtcgca
     3121 tgcttaatta ccgtacctat gtagatttga tggatctgtt aagtcgaata ccttctgtta
     3181 ctgagccagg gatgactgct gaagaagata cgagagattt tgatgcaaaa caccgaacct
     3241 ttgacaaagc acgtttgatg gaaggtggca aaggaatcat tgatggcgga aaactgggat
     3301 tcaataataa agaccgtatt cttctgacta aactgattgc tatgcctgat tcagaagaag
     3361 aaaaactaga caatatcact atcgaagaat atttcaaaga tgatccacac ttttttgaaa
     3421 caaacttctg gtttatgtgg gaaacaacat ttgctttcag aacacgtagt tctgctcaag
     3481 aattgcgtcg ctatatgcat caaatgattt atgaattcac tcaaatcgaa catctagtgg
     3541 gcgtcaatcg aacacgttac aatcaatacg aaagtattat gcttcctttg atcgagtact
     3601 tgaaagaaca aggatgcaaa atcattttga accgccgtgt cactgactgg gaattcaaag
     3661 aaacaccgat gcaagatgaa atcactgtga ctggtttgaa aattaccaat acactgactc
     3721 aagaagaaga atacgttgtg gtcgatgaag atacagcagt tattttcact aatgggtcca
     3781 ttacagattc agctacttta ggagattacg aaacaccagc tgtagaaaat atggattatg
     3841 gtgcagcttc tagtctctgg aaaaaagcca gtgaacgttt ctacaacttg ggaaatcctg
     3901 acaaattctt tgctgatcga gatgcaagcg aatgggtcag ctttaccctg acgacgaaaa
     3961 accatttgtt gttgaatgaa atcacccgta tcacgactca agaaccagga aatgccttga
     4021 attcatttat ttcaaccaca ccgatcacgc cgttaggcca aaaagatgtc aatatgtcta
     4081 ttgtcgtgca tcatcaaccg cattttacgt ctcagaaacc aaatgaaaca gttatttggg
     4141 gttatttcct ttatccaaga cgacgtggcg aatttgtgga taaacagtat attaaaatga
     4201 atggaaaaga aatgctagaa gaactgattg gacaactttc aaaagtagat cctggaccag
     4261 tgaatattcg tgaaaaagaa acagaaattt tcgacagcgt aataaataat atccctgtct
     4321 atatgcctta tgcttccgcg ttgttcaata atcgtgccaa aagcgatcga ccaaaagtca
     4381 ttcctaaaca ttcgaccaat ctggccttta caggagaatt tgtcgagcag ccttatcaga
     4441 tgatctttac tgaacaaagt gctgtacgtt ctggagaaat cgctgctttc cattttgcgg
     4501 gtattcctat gtcccgtctt gtcaaaacac cgcgttacga caaagatatt cctactctag
     4561 ctcgtgccgc aaaaaaaatg tttgaataat caagaggttg tgaaaaagct gccctgcatc
     4621 aagtaataag aacaaccagg ctaaaacagc ttcttatgtt tttgatttgg ttggacttat
     4681 taccgcagat gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa gctgccctgc
     4741 atcaagtaat aagagcagcc aagctaaaac agcttcttat gtttttgact tggttagact
     4801 tattaccgca gatgcaagct tttgaacacc gtttaccaag aggttatgaa aaaggcgttt
     4861 agtttcgagt atgaagaaaa attttgtgaa aatggttcct cacgtttttg cataaatttg
     4921 acttaatacc gagaaactga tcccttctgt cataacctct ttttatttat gtctgtttct
     4981 ttttgaaaac aactattttt tcaacgttta ctctcttgcc aatccaacat catctaacgg
     5041 atacaactat ttcaatgatt accattacac taaaaaatcg gaaacacacc tctccgaaca
     5101 caaatacata agactataga atgtttcatt taagaaaaaa agcgtttcat tatcaaaaag
     5161 gtaagatctc ctttaaaatg aaattatctg tagtactaat aatgcagaca attttatgaa
     5221 tctgaaagga gcatctccaa tgaaagcagt cacttggcaa ggaaaacaga aaatggaaat
     5281 cagaaacgta gatgatccaa aaatattaga accaacagat gcaatcatcc gtatcacagc
     5341 aacagccatt tgcggctccg atctgcatct ttaccatcat ggtgaagcag ttttggaaaa
     5401 agggtatgtt gtaggacacg aaccaatggg aatcgttgaa gaagtgggat ctcaagttca
     5461 aacattgaaa aaagggcaac gtgtggttat cccttttaat attagttgtg gtcattgtca
     5521 tttctgcctg aatcacatgg aaagccaatg tgatgaatca aatccagatc agttatatgg
     5581 cgggcttttc ggatttggca agttgaacgg taatcattgg ggtggtcaag cggaatatct
     5641 aagagttcct tatgcagact ctacttcatt tgttgtacca gataatgatc ttcctgatga
     5701 aaaagtttta tttctatcag atattttgcc cacagcttat tggagcgttg aaaacgcagg
     5761 agtaaaaaaa ggcgatacag ttgtgattct aggttctggt ccagtcggtc tattcgctca
     5821 aaaattcgct gttatggctg gagcagagcg cgtaatcgca gtagatccag tacagcaccg
     5881 tctagataaa gcggctaatt acaatggggt tgaaacttat ttcttagaag acacggcaca
     5941 agctggaaca gaattgtatg aactggctaa aggtggcgca gatgtcatta tcgattgtgt
     6001 aggaatggac ggattagagc ctgtcaaaga aaaagcgaaa aatttagtaa gcttacaaag
     6061 cggcacaatc tctccattac agatggcatc gcaagctgtc aaaaaatttg gaacagtcca
     6121 agtaactggt gtttatatga ctccagcttc ttcttacccg ctgcaagaat tttttatgcg
     6181 ggatatcgct gtcaagcatg gacaggcacc aattatccat ctaatgccaa aaatttacgc
     6241 aatgattgca gaaggactat tcgatcctac acaaatcatt actcattcgc tgccactatc
     6301 agaagcagca caagcatatg agatctttga caaaaaagaa gataaaaata taaaagttgt
     6361 tttgaaacct tgaattcagt aaaaaataca ggtgattttc tttatgccca aaatcaaacg
     6421 aaatacccat gagatcgaaa taggaaaaga agaagatatc gaaatacgag gacgtcgctt
     6481 ccgcttctat ttccaaaatc gttacacatt gatctcactg gctgtcgatt tattaaccgg
     6541 gattttctat atttttggaa gccttgcttc cttaactaaa atccccgatc gatatggcac
     6601 ctatttttat ttggctggtg ctattttctt gacgattcgt cctattctca gaatcgtaag
     6661 gaacattttt atttacgacg aaaaaaaagc aatgaagaga acaagcaaaa aaaaagaggc
     6721 atgagaattt ttcaaatctc atgcctcttt tttcacccta tcgtatcact ttgatacaga
     6781 cgagcggtcg tcccactttc ttccttgacc attgtcaaaa atgtccactc gtatcgttca
     6841 taaaacgtgg tatgatctgt aattaagtaa agtttttcgt acccaagttg atgcatttct
     6901 tttctgattt ccgctaatat ccgcttggct atcccctgct tacgcttttc cggttcaaca
     6961 aacagggcac aaagatttgg tgtcagatca gggcggttat ggaagtcgtt ttcaatgacg
     7021 cccgctcctc cgacgatggt ttctttttca tctaagacca cataccattg aggaatactt
     7081 tccttattag cgattccagt acgaatactt tcctcataca caacagcagg aactccccat
     7141 tttgatgaaa accaattcgc gctttgtttt actaattctg ggtgttctct taatttgatc
     7201 cagttatatg taaccaatgt ttaaacctcc gactaaaata aaacgtttac aaaaattata
     7261 caagtcttta tgaaaattag caatgacaac aatagtgatg taaaaaaaaa aacggaaaac
     7321 aatcacttgt tctccgtttt ttgattaatc aagcatctaa agcttctttt cttctttctt
     7381 ggatcgtagc taccttgacg aacggtagat agatcaaaat agaaagaacc aaattgacag
     7441 cggctagtac accacctgca atactttttg ttgccaatac accgccaata attggtggag
     7501 ttacccatgg aggcataacg gtagctgctg gcactaaacc tgtagaagtc gcaaagtaag
     7561 caacactaac taagaccatt ggtgtcaaaa taaatggaat gaacatgatc gggttcaaca
     7621 cgataggtaa cccaaacatc gttggttcat taatattgaa gacacctggc gcaatcgaca
     7681 agtttgttac aaccatatat ggtttgtttt tacgtcctac taaataaata gctaacagta
     7741 aacctaaagt tgcacctgtt cctcctaagt tgacaaagct gtcaaagaaa ggtttgttca
     7801 cgataaacgg tgcgctatgt ccagctgcga ttgcattaac gtttgcttca atggctggcg
     7861 cattgattgt ttgcatcaaa ggatcgacca tattcgctcc gtgtaaacca aagaaccata
     7921 agaatggtgt gataaatgca agcagtaagg cagatggata agagtttgca agtcccataa
     7981 atggttcttg gaccactgta tagaaagaac ctacgatatc atcaacaccg aatgcagcaa
     8041 aaattgcagc aactaaagca aacgcagaga tagtgatcat tgctggtagt aatgaagcaa
     8101 atgcttttgc aactgccggt ggcacaccat caggcatttt gatgatcaat ttttcatttc
     8161 cgcttaaacg tacaaagatt tctgttgaaa taatcccaac aataatagca ataaacaaac
     8221 cgtttgagct catacctaat gctccgccta aagcgaagaa agagcctaaa gaaatcacac
     8281 ctgcggaaac accgtctttt cggtagccgt tagctaaatg ataagcaagc aagaaagcaa
     8341 tcaagacaga aagaatggca aatgtaccgt tccaagcagc agccccgaat cctttccatg
     8401 attcaccacc aaaaattgag ttcatcaatt cttggaaccc gggaatcggt aagttattga
     8461 tcattactgc taatgctcct aagatcatta acggcatact caccataaat gagtcccgaa
     8521 tcgcaactaa atgccgttgt gcaccaattt ttgatgctac aggtataaaa tgtttctcca
     8581 taaatgcaac aaatttatcc ataaatcttc cctcttttct ttactgtaga ttacgcgttc
     8641 aatcgacgat acagattaat gatctctttt gctaaatctg taaaagctat agatgtcatc
     8701 agatgatctt gactgtggac cgtcaaaagc gtgacttcaa cgtgatttcc ttgtgcttct
     8761 tgtgtcagca tctctgtttg agagtgatgt gcttggacta aagattcctc tgcatctttg
     8821 attttttcat ctgctaaagt gaaatcacct tttttcgcag cagcaatcgc ttccattgca
     8881 tcgctttttg catttccgcc aaacatgatc agacccatga tggcttctag atttttttga
     8941 tcttccattt attgtatcct ccgataattt ttattatttg tttattagtt tttctgcttg
     9001 gtcgagtact ttttcaccat tcatcatgcc atagtcagcc atattgatta catctaaagg
     9061 aatgccttta ggtgctagct tttcagcaaa ttgattttgc ataaagcgta cttgcggtcc
     9121 taaaagtaaa acgtctacat ttttagattc tagttgatta tccgcatctg aagcagatac
     9181 agcaaagatg tctgcttcca ttccttttgc ttctgctgcc ttttgcattt ttgtgactag
     9241 gagacttgta ctcattccag ctgaacatac aagcatgatt gttttctttt ccattttaaa
     9301 cacgttcctt tctatactta cacttgtata taagcaagta gcgtgccaac tttaaaagcg
     9361 ctttaaaaag ttgatatcat cacatttaag gtctatacca agttgcatac acactaaaaa
     9421 agaaagcgca cactaaacac acaaattaat ttttattacc ttttttcttt taacgtgcac
     9481 aaaaaaagtt gtgacacaaa acttgtcaca acttttttca aataaacggt attcaaaagc
     9541 ttgcatctgc ggtaataagt ccaaccaagt taaaaacata agatgttgtt tttactttgc
     9601 tattcttatt acttgatgca ggacagcttt ttcacaacct ctcaataaac ggtgttcaat
     9661 cagctgaccc tgggtattag ctcaaaacgc tggattcaca acctctttta tttcaaataa
     9721 ccagtaaata caatgaacat ctacaaaaaa cttctattta tttatagatt tcgtcaggca
     9781 aatccaattc tctcttatca atcaaagtct gataccacga agcagatttt ttcaatgttc
     9841 tgcttcgttc atcgttcaat tcgattcgaa ctaatccata tcgattctta aatgcattca
     9901 ttggtgacac acagtcagtg aatgcccata acatatagcc ttcgcaattt gcgccttctt
     9961 ctgttgcttt gatcaagtaa tgcaaatgct gtccgataaa ttcgatacgg taatcatctt
    10021 gcacgattcc tttctcatcc atatactgtt cttcatattc tcttcccata ccgttttctg
    10081 ttatcagcca agggatatta tgataatttt cttttagata catggctata tcatacatga
    10141 ttttcggata gatttcccat cccctggaat tattcatttt cttcccaggt aaatcaaaga
    10201 cttcataata tcgttcagga tggaaaggtg tctcttcatt ccattgataa cgaggggcgc
    10261 gaacgcgttt aggataatat tgattgattc caaggaaatc tactgtatga tgctgaatcg
    10321 tttctaaatc ctctgttgca ggatcgaaat aaatcgattg tttttgacac aactccagga
    10381 actcttctgt gtagtatcct tttaccatcg gatcaaaaaa tactcgatta taaaacaaat
    10441 catacatata agcagctttt tggtcttctg gagaattgga tcgagcatag accatttcag
    10501 gatttaaaat acagccaatc cttccaccat aatttccgct gcggtaaatc tctactgctt
    10561 tagccgttgc taaaacttta tgataattcc acatcatcca cttttttgtg ttttgttcat
    10621 gcggccagcg aacagcatcc aaatagcaac gcgtttgggg aacgattggt tcgttgaatg
    10681 taaaccagtt ttttacccta tcaccgtaac gatcaaaggc aatttgagca tacttagcat
    10741 aaaaatcaac tacttttctt gaagaccagc catcatattt ttccaaaaga aaagctggga
    10801 cttcataatg ttccaaacaa atcatcggtt ctacgtctgc ttcgatcaaa gagtcgatca
    10861 tttcacttat atgctgagca tattcttcat ctactataca gttctcataa tcagtaaaaa
    10921 aacgagccca attgatagaa gtccgataat gtgtcagatg gatggatttc atcagatcca
    10981 catcctgctg gtattgttcc ataaaattag ttgctacggc tgggccatac ccattatgcc
    11041 aaacaaaatg ttcattttta taccagctat ctataaacga atcctgtcct tctttttttc
    11101 ctttccaacc ttccgtttgc caagcagaag atgcagcacc aagaataaaa ttttttggaa
    11161 tagtaatttt catgatttcc tcctgttttt tagatcccat cattttccac acgatttgct
    11221 acttttacaa atggcagata gatcaaaata gaaacaataa tacaaacaag ttgcgtaata
    11281 actgcaccga tatttcctgc tgttgccaga taagcattga cgatcggcgg cattggccaa
    11341 gggaccatta caactgcttt cgccgcaaat cctaaagcgg ttgcaaaata accaatcgtt
    11401 ccagtgataa gaggcgttaa gataaacgga atagctaaaa ttggattcag catgatcggc
    11461 ataccaaaaa taactggttc attgatgttg aagatcccag gagcaaggga taacttggtg
    11521 atttgtttca tatcttcccg ttttccaact aacaatactg caatcagtaa accgatcgtg
    11581 actccagacc cgcccatact catatacata tcccaaaatg gcatagtaat aatgttcggt
    11641 acttcttttc cagcttcaaa agcttccgta tttacagcaa ttgccgctaa aagcaacggt
    11701 tcccgtatcg gcttcaccat ttgatttcca tggataccaa tcacccaaaa gacttgcgca
    11761 ataaacatca atagcaaaat acccggtaac ccttgtacga caccttctaa tgggcgttgt
    11821 acgacattgt aaatgatatc ataaaggtac attcctgtaa gttctttgat gataaatccc
    11881 gcagccgcga tacccgatac agtcagaatc gtcgggaata aagcagaaaa actagcagaa
    11941 acattcgttg gcacggtatc aggcattctc agctgtagtt tcttctgttt cctcaaccag
    12001 caataaagtt caatagaaag gatggagacc aacattccca aaaataaccc tttcgtatct
    12061 gtatattgtt tagccaatac gttttctact ataacagtct gattttcatt cactaattca
    12121 taaacagttg gattaacaga taaataagac atcactgcga tgataccagg gaatacacca
    12181 tccactttat tcagcttggc taattcgatc cctatcagaa aaacagcata gatggtcaag
    12241 aaacttagtg tgacatagct gatggcagat gcgataggct ttaattcttc taaaaaacgc
    12301 aagaaactta tctgtgctaa gccattttcc gagtcaaaga ccattgctga aaacaatgtg
    12361 ccaaaagcac ccgtaataat taccggaatc aatgcagcaa atgcgttttt tatcaccatg
    12421 atatacctta aattattgat tctcgttgcc acgttcccta aaacattggc taatttatcc
    12481 ataaaactca ttccagatcc cctcattcct gcttcttctc cttgacgtca tctctttttg
    12541 tctattagtc agcgcttaca tttcgatgaa cagaaactat ttactcctct tttttaccaa
    12601 atataaaaca aaaattcatc tgatgtattt atcataaaat acatcagatg aactgtcaat
    12661 tagtttcttt ttctcctatt attttctgta attctctccg atttattgcc atatggacag
    12721 tcattgcatc aaacgcagca agtgcattcc gcctttcgat ggcctgaacg atctctgtat
    12781 gaatatcgat cgtctcgatt ttcagatgct ggatatcata atttttatta aacaaagcaa
    12841 ttgattcatt aatgattggg ataatatgca gcaaggctac gtttccgcta gcttctgcaa
    12901 tcatcgtatg aaaatcgatg tctaattgaa catgttcttg tgtgccttct cgaaaagaag
    12961 tttcaatctt ttgcgttaat tttttcattt ggacgatttg attcttcgtt gcatattccg
    13021 ctgccaaacc tgccatctta ggctccaata aatatctcag ttcaaataag tctcgtatca
    13081 tccgatcctg atcttcaatc aaagaaaaac cgagtgggtc attggcaatc cctcgcttat
    13141 cactgacaaa tgtccccgct ccttgccgaa cttcaaggat attcctggaa gctagagccc
    13201 gcacagcttc tcgcaccgtg cttcgcccca cctctaaata atcggacaat tcatattcat
    13261 tcggcagttt atctcctact tgcaactgca tttcctttaa atatctaagg atcttgtcag
    13321 tagttacttc tactagggtt ttattttctt ccaaaccaca tacctccttt tcatctgatg
    13381 tattttttcg taattattat aacacgtttg aatcatctga tgattttttg tatacaaaat
    13441 tgattaatcc gttcatagac atacaaacga agaagatatt taccataaaa aagactagga
    13501 taaaattcac tcctagcctt cgatcatttt tattttgaac gacaaaaaat atgatgttca
    13561 ataacagacg atcttcattc gattcttgat gtcatctttt gtttttttta atatatggat
    13621 aaaaagtttg attacatcgt ttctattcta gaatttggtc gattctcaat tcttcttctg
    13681 gtgagaaatc taaattatcc atcattcgaa cactttcttg taactgactt aaccgacttg
    13741 ctccgaccag tacacttgta acagattttt gtcgcaaatt ccatgcaagg gccatttgcg
    13801 ctaatgattg tcctcgtgat acggcgattg tttgcagtgc tttgattttt tccagagttg
    13861 atccaacttg ttcttctgaa aggaatggta tttccttacg atgtgctcta gaatcttcag
    13921 gaataccatg tagataacga tttgtcagca acccttgagc aagcggactg aacgtgattg
    13981 cccctagccc ttcttcttct aatactggag atagatcgtc ttctagtagt gcacgagaaa
    14041 acatattgta gcgcatctga tgaataatga acggtgcttc ctttcttttt aaaatttcag
    14101 tcattttttt tgtatcttct ccattatagt tggatatacc aatatataat gcttttcctt
    14161 gtcttacaag ttgcatcaaa gcttctgctg tttcttccaa aggtgtatca gggtctgggc
    14221 gatgatgata gaagatgtca acataatcta gccccattct ttggagactt tggtcacaac
    14281 ttgaaatgat ggatttacga gagccccatt ccccatatgg cccttcccac atatgataac
    14341 ctgctttgct agaaataatc agttcatcac ggtaggcttg aaaatcttct ctaaaaatac
    14401 gcccaaagtt tttctctgca gctccagcag ggcctccata attattcgca aggtcgaaat
    14461 gggtaattcc catgtcgaat gcgcctctta aaatctctct ttgattatgg ataggatcat
    14521 aatcaccaaa attttgccat aatccgagag ataaaatcgg taatttcaaa cctgaatgac
    14581 cggatttacg atattccatt ttttcataac gtgcactatc tgcaatatac aatttttctc
    14641 ctcctcatca aatcttttta aaacgctttc ttttttctat ttttagttta ctacatttta
    14701 aagaaaagga gttaatcaaa atgatttttt cgtatatttt tttgccattt gttttttact
    14761 cgttgataga tcagaaattg attacgtgct tgctggattt ttttataacg tttcggttgt
    14821 tttttgtaaa attgctgatg atagttttct gctggccaaa acgtaccggc tggttcgatt
    14881 tttgtgacga ttggttggtg gaaagttcct gaactgtcta gtttctgttt tgattgttcc
    14941 gctaattctt tttgtcgttc gacttgataa aaaatcactg gacgatattg cttccctcta
    15001 tcttgaaatt gtccaaatgt atctgtggga tcagtaattt gccaatagat tgtcagcaac
    15061 tcgctataac ttatttctct tgtgtcataa atgatctcca cagcttccac atgacctgtg
    15121 taaccaccac ttacttgatc ataatttggt ttttcaacat gtccgcccgt gtatcctgac
    15181 aaaacagata ggatcccttt tttcgattca aacggctcga ccatacacca gaaacatccc
    15241 cctgcaaata ctgcacgttc ttgatagtca gcatcttgca tggcatgctt ggaaaagtca
    15301 tatttttgct ccttatcaaa tccttccgta atcaataaat aaaagtccat tacatcaggc
    15361 gtcagattca ttcggatagc taatggacgt agtgttgctt ctagttttga taactgttct
    15421 ttaacggata tattttctct tatgtcattc tttgtctgaa tcaatacttt tcgttcccaa
    15481 tctcgtgtat tcggattcaa aatcagatta taaagtgttt gaatgaatat ttgttcctca
    15541 tccatagatt ctcctccttt ccgaagaaga caagctagtg actttagctg taatttatca
    15601 taaagtgagg aaataagcga ttacttcatc cccctacaga tttttcgtat aaaaaagaga
    15661 gaatccttat cgacagattc tcctttttaa tcaatttatt ttataaatga aaagttaatt
    15721 taccttttat ggaaaactat tttaaaaaat aaacataaat atgcagttaa tgtaacaaga
    15781 taagaaataa tagtcgttac tgtataactt gtgcttactt taatatcagc aactcctttg
    15841 actccgctta tttgtaccaa tccattagca ttcacctgct cctttacagg ttgatgattt
    15901 atttctacat tcgttcctaa ataagcatat acaggaagtg tgattacttg gctcttctca
    15961 ttagttactc tatatatgat agaactacca gttgttttat aaggaaggtt tacccattga
    16021 ctgccaatct ttgcttcgtg ttttattata tgctccttat tctccaatga agcgatagga
    16081 gaataatcat ataaaaaata atccattcct ttagtggtat acgcctctac tgtttctttg
    16141 tttacaacaa tatgtcccgg ctgagaaatt attttttgat tgaaattttt tttactagaa
    16201 acagttaaag tgattaataa caaaacagtc ataaataaat aaaatagttc tcttgtctta
    16261 ttgtatcttc tatttttgaa aaatatcatc gttaatatca gagaagagaa tattatttgc
    16321 aagcttaaga tccgccaagg aaattgtaac aatttggctg gcgtattttg cagaagatac
    16381 catggcacca aactagttgt agctagcc
//
LOCUS       DARDHA010000051        16373 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000051 DARDHA010000000
VERSION     DARDHA010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.051"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            135..2606
                     /locus_tag="SDA10_001338"
     CDS             135..2606
                     /locus_tag="SDA10_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG4240256.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2709..4445)
                     /locus_tag="SDA10_001339"
     CDS             complement(2709..4445)
                     /locus_tag="SDA10_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEG4240257.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4442..5146)
                     /locus_tag="SDA10_001340"
     CDS             complement(4442..5146)
                     /locus_tag="SDA10_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4240258.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5277..5780
                     /locus_tag="SDA10_001341"
     CDS             5277..5780
                     /locus_tag="SDA10_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEG4240259.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5740..6078
                     /locus_tag="SDA10_001342"
     CDS             5740..6078
                     /locus_tag="SDA10_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEG4240260.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6099..7517
                     /gene="ffh"
                     /locus_tag="SDA10_001343"
     CDS             6099..7517
                     /gene="ffh"
                     /locus_tag="SDA10_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEG4240261.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7629..8207
                     /locus_tag="SDA10_001344"
     CDS             7629..8207
                     /locus_tag="SDA10_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEG4240262.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8211..8732
                     /locus_tag="SDA10_001345"
     CDS             8211..8732
                     /locus_tag="SDA10_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG4240263.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8811..9365)
                     /locus_tag="SDA10_001346"
     CDS             complement(8811..9365)
                     /locus_tag="SDA10_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG4240264.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9618..9893
                     /gene="rpsP"
                     /locus_tag="SDA10_001347"
     CDS             9618..9893
                     /gene="rpsP"
                     /locus_tag="SDA10_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEG4240265.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9905..10156
                     /locus_tag="SDA10_001348"
     CDS             9905..10156
                     /locus_tag="SDA10_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEG4240266.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10281..11072
                     /locus_tag="SDA10_001349"
     CDS             10281..11072
                     /locus_tag="SDA10_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240267.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11242..11763
                     /gene="rimM"
                     /locus_tag="SDA10_001350"
     CDS             11242..11763
                     /gene="rimM"
                     /locus_tag="SDA10_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEG4240268.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11753..12514
                     /gene="trmD"
                     /locus_tag="SDA10_001351"
     CDS             11753..12514
                     /gene="trmD"
                     /locus_tag="SDA10_001351"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEG4240269.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12650..12997
                     /gene="rplS"
                     /locus_tag="SDA10_001352"
     CDS             12650..12997
                     /gene="rplS"
                     /locus_tag="SDA10_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEG4240270.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13129..14265)
                     /locus_tag="SDA10_001353"
     CDS             complement(13129..14265)
                     /locus_tag="SDA10_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4240271.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(14381..15295)
                     /locus_tag="SDA10_001354"
     CDS             complement(14381..15295)
                     /locus_tag="SDA10_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEG4240272.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(15386..15814)
                     /locus_tag="SDA10_001355"
     CDS             complement(15386..15814)
                     /locus_tag="SDA10_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEG4240273.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(15819..16193)
                     /locus_tag="SDA10_001356"
     CDS             complement(15819..16193)
                     /locus_tag="SDA10_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4240274.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
BASE COUNT         5279 a         2951 c         3219 g         4924 t
ORIGIN      
        1 cctttttttt tgctatatga caggaatgaa aggtgtttgc aagcttaatg tgaggaacac
       61 tttccatatc tgttctatac tggataggag aagtttggaa atatccagaa aacgaaatca
      121 atcgaggtag ccacatgaag aataaatgga taaatcgaag taaaaaaaag aaagtaacac
      181 aaagatcatc agataaagtc atccgtcttt ttccatcgta taaagaagga ttaacggcag
      241 atcaagtcaa ggaacgaata gaaaaaggtg cagcaaataa ttcagtcgat cccacattca
      301 aaacaaatca gcaaatcgta ttggaaaaca tatttacgta tttcaatttg atctttctta
      361 tcttagccat tttgctttgt ttagtagaat cctataagaa cttgactttt ttaccagtta
      421 tcatagccaa tacaggaatc gccatttttc aggaaattcg atccaaaaag atcttggacg
      481 aactaaatgt tttaaataca gcaaaagtca atgtcatacg agacgggata gaacaaacaa
      541 tagatataga agaccttgta atagatgaca ttgttatttt agagactgga tatcaaattc
      601 cagcagatgc agaggtcgtg gaagggaaac tgcaagttaa tgaggcttta ttgacaggcg
      661 aagcagatga aataaccaaa gaaataggtg atccattaat gtccggcagt ttcattgttt
      721 ccggaaaagc ttacgcccga ttagacaaag ttggagcaga ttcgtatatt tcccagctga
      781 cagtcaaagc caagacaatg ggagaaggag aacagtcgga gatgattgct tctcttaatc
      841 aattgatcaa atggattggg atcatcatta ttcctatcgg tatcctgctt ttttctcaaa
      901 gttacttttt taatggaaac acgatcaaag aaagtgtcgt tgcaatggaa gcagcattga
      961 tcggtatgat acctgaagga ctgtacttgc tgacgacgat tgcattagca ttaagtgcga
     1021 ctaaactagc aaagcaaaga gttcttttac acaatatgaa aagcattgag acattggcaa
     1081 gggtaaatgt cctttgtgta gataaaacag gaacgatcac agaaaataaa atgtctgttc
     1141 aaaaaatgat cgtctctaaa aaacaagaaa taatcagtca gtcagatcaa ttggaagcat
     1201 gcattgctga ctatgcaaaa gcgatggcag gagacaatgc aacaatggaa gctgttaaag
     1261 catattttac aaaaaccact gatcaatcct atcaccatgt tattcctttt tcttctgtcc
     1321 aaaaattcag cagtgtagcg ttggcagaca aggtgtatgt tttaggagcg cccgaaatgg
     1381 ttttgcgcga tcaattgccg gaatatgcgg aagagtttgt ttctttcgct gaaaaaggct
     1441 atcgcgtgtt ggtctttggt gtgtacgatg ggattcttga agaaccagta ttgacagaag
     1501 cagtcacacc attaggctat attctgattg ctaatcctat cagaaaagaa gcaagagcca
     1561 catttgatta cttcaaaaaa cagcaagtag ccatcaaagt gatttctgga gataatccgc
     1621 tcaccgtatc caatgtagct gtccaagcag ggatttcaca tgctgatcag tacgttgatg
     1681 catctaaatt atcagaagaa gaatatgaag cagctgtcga aaaatataca gtttttggac
     1741 gcgtcaagcc agaacaaaag aaaatcttcg ttcaattgat aaagcgtaac aatacagtag
     1801 ctatgacagg tgatggcgtg aatgatattc tagccatgaa agaagcagac tgcagtatag
     1861 cgatggcttc cggaaatgaa gccgccatgc aggcctcaca agtggtctta cttgattctg
     1921 acttttcacg catgccagaa gttgttgcag aaggacggcg cgttgtgaac aatattgagc
     1981 gatcagccag cttgttttta gtgaagaata ttttctcctt tttgctctca ctgttttcag
     2041 tgatctttgc tctgacctat cctttagaac catcacaaat cacattgatt agtttgttta
     2101 ctatcggact tccatctttc ttgcttgctc tagaagaaaa caaaaagcga atcagaggga
     2161 aattcatcat gaatgtgatg gaaaaagctg tgccaggtgg actaacagat atgatcgtag
     2221 tcggtgcgtt ggtgatctgc ggggtgactt tgaatctaaa taaaacagat gtttctacag
     2281 catctaccat gcttttgatt gcagtagggt tcctcgttct ctataaaatc tgttcaccgc
     2341 tgaataaatt cagaagtcag atcattcttt tctgtgctag cggtaacttt ttttcggtca
     2401 tctttttgca taagctgttt tcaatcaccg gtatctcagc tgtatcactt ttgctgttga
     2461 gcattctatt tttctcggca gattcgattt ttaggcattt gaccacattg gtagaatatt
     2521 tgtttgaacg taaaaatgaa aaagagcaaa agccgtttag tcgcgtagct aaaaaatttc
     2581 tatcgatatt taaacgagaa aattgacgaa aagtatctca aaaaagagta aaaaagaaca
     2641 accactcgtg tatctttcga agcaggatga gtcatccaac tttaaaacgc acgagcagtt
     2701 gctctttttt acattttcgg aatggtaacg gtgaaagtcg aacctgttcc aagatgactg
     2761 tctacagtaa tcgtaccatt aaggagttgc acgtagtctt ttacaatcgc taaccctaat
     2821 ccggtcccgc ctgaatgtcg gcttcgcgcc ttgtctacgc ggtaaaaacg ctcaaagatc
     2881 cgttcctgat cctcaatgtc gatacctata ccaaagtctt ggacagaaaa cgatagatca
     2941 tctgttgcct gatagcggat aataatctcg ctgtcgcttt ttgaatattg gacagcattt
     3001 tcaattagat ttttgataat tggataaagg atatctgttt tagtagtaaa tggttcgttc
     3061 tttccaatca atcgaatcgt caaccgtttc ttttcgatga ttggctgata attttgaacg
     3121 atttgctgga aataaggttc tgtataaatt gtctggatct cgtaagaatt ctcgccatct
     3181 tttgacagct ggatgatttc ccgaatcagt ttatctaacc ggatcgcatc tttttgcatg
     3241 atctcaagaa aagaagtcag agtttgcggg tcatcttttg caccatccag taaagtctcg
     3301 gtaaaaccaa tcagtgaagt cacaggagtt tttaattcgt gagagacgtt gccaacgaaa
     3361 tccttttgta gcttttctaa acgtcgcact ttcgttaagt catacgccac ccctagaatc
     3421 tgtccggtac cttctgaatt agtaaagtag cgcaaactca agtctaatgt ctgccgacct
     3481 tctgtaaggg tgatctcctg atggatcagc ggatgctcag gagtcacttg atgaatcagc
     3541 tgaatgagtt ttggctcttg aatgacttct gtgtaccgct gttcaggttg atagcttacc
     3601 acgtttaaat gtagctgcat ctttggatta agcagtagta aacgagaatc agcatcatca
     3661 atcaggaata cgccaatcat cagttcgttc agtaacgtat ataattgctc ttctgtggta
     3721 gtacacgccc gatacatctt actcatttgt tcgctcaacg cattaatcgt ctgatacaat
     3781 tcttcccatt gatcgggtgt ttgcatgatt acttccgcct gattaggatt ttgcaccatt
     3841 ttttttagta cgggcagcac ggttttcaat ggctggtttt tttgatagat cagataatag
     3901 atcattcctg caagaacaag gaagaaagac agaaagaaga aaaagaccca acggcgaaaa
     3961 ctctcagttc tcggtaaaaa accgcttgtc ggttctgcga tacgtaaaat ggcttctaac
     4021 tggccacttt ttttgactgg taatgcgaca tataaaagat cctcattcaa agtagtactt
     4081 ttgcgcaaag cgctacctag gttcccacca ttcaatacgg ctttgatttc gggacgggag
     4141 tttcgctgtt cattcaatgt ttcattgttt gtgtcaaata agatctttcc agtagcatcc
     4201 attaaagtaa tccgttcgtt cgactgatgg acgaattctt ccaaagcagt tttgttttgc
     4261 gctgcttgga ggttttcgac atctaattgc cggatcagca gttgtgcttt ttttgtcagg
     4321 tagctttctt gctgttccac gatctgctgt tgaaaataat gggaaatcaa ttgccagcca
     4381 ccaaaaaaca aactaactaa aagaataaga aagaagagac gttcataaat ttttttccat
     4441 ttcatcgttt gggttcctga aacttatagc caaatcctct aatagtcacc aagtactttg
     4501 ggtgttttgg atctcgctcg attttttctc gcaaatgact aacgtgtaca tctacgattc
     4561 ggctttgtcc tgcgaagtca aaattccaaa tgcggtctag caatgtgtct cggtcgatca
     4621 cacggtcttt tcgtttcatg aaataaacaa gtaattcaaa ttctttcggt gttagttcaa
     4681 tcagatggtc gtctacggtc acttgataat tggtcaaatc agcagtgatt tgtccaatcg
     4741 atagatattc aggctcagct tcatcttttg attgcttacg aggttcaatt cgtctgaata
     4801 ttgctttcat acgggctaat acttcgcgag gactaaatgg tttggtcaaa tagtcgtcag
     4861 cacctatttc taggccaatg atccgatcca cttggtcatc tttagccgtc agcatcaaaa
     4921 tcggtgtttc atttttttct tgacgcagcc gctgagtgat ctccatgcca tccatcgttg
     4981 gaagcatcac atctaaaatg atgaaatcaa atgattgttc taatgctaat tctaatcctt
     5041 cgcctccatc tgtggcactg gttaccttgt agccttcttt ttccaaattg aacgtcaaga
     5101 gcgttacaat agaaggttca tcatcaacaa ctagtatttt tttcattcaa atctccttta
     5161 aataggtttc attctgttta tattttagca tagaatcagt acaaagggct attttacaaa
     5221 agaaataaat ggtataatag gaaggcactt tgctgaatag aaaggaagag cagtcaatga
     5281 ttggaatcag tgcatgttta ggcggtctgg cctgtcgcta tgatggacag aaaaaagaag
     5341 taccagaatt gatggaacta gttgaaactg gagaagctgt catggtctgt ccagaagtga
     5401 tcggcggatt accaattcct agagatcccg ctgaaatcat tggtggaaat ggattcgatg
     5461 tgtggaaaga tcaagcaaaa gtatggacga caagcggcga agatgtaacg gaagcgtaca
     5521 aaaatggcgc gatcaaagcc tatcaaaaat tgcaggaaaa acacattgat ctagtcatca
     5581 tgaaggaaaa aagcccttca tgcggaaccc attcgattta tgacggaagt ttttccggaa
     5641 taaaaaaaaa tggtgccgga gtggctactg cttattttat tcagcaaaaa atgactgtgc
     5701 tctctgatga agaatggctg aaaactagag gtgagcagaa tggaaatcga aaagaccaat
     5761 cgaatgaacg cgctctttga attttattcg accttattga cagaaaaaca aatgaactac
     5821 atggaattat attatgcaga tgatttttca ttaggtgaga tcgcggaaga atacgaagta
     5881 agccgacaag cagtatatga caatatcaag cggacaagca agatcttgga ggattatgaa
     5941 aaaaaactcc atcttttttc taattatatt gttcgagaac aggtattgga aaatatgact
     6001 gcatatatta cagataagta cccggaagat aaaaaactac ttgaatatgt acagcaaatt
     6061 caagcaatcg aagaataaat aaaaggaatg aagaaaatat ggcatttgaa agcttaactg
     6121 aacgtctcca gcaggcgatg ggaaaaataa gaaaaaaagg aaaagtatca gaagctgacg
     6181 taaaagaaat gatgcgggaa atccgccttg ccttactaga agccgatgtt aacttgcaag
     6241 tagtcaaaga ttttactaag cgcgtacgcg aacgtgcggt aggggcagaa gtgctggaaa
     6301 gtttatcacc tgcacaacaa atcgtaaaaa tcgttgatga agaattgacg atcacacttg
     6361 gttctgaaac agcagagtta aataaatcac caaaaatacc aactgttatc atgatggctg
     6421 gtcttcaagg ggcaggtaaa acgaccttta ctggtaaatt agccaattat ttaaagaaaa
     6481 atgaaaatgc tcgtccgctc ctaatcgcag gggacgtata tcgaccagct gcgatcgacc
     6541 aattgaaagt attaggtcaa cagctagacg ttcctgtatt tgatatggga actgaagtca
     6601 gcccggtaga gatcgtccgt caagggatgg agttagcaaa agaaaagaaa aacgactatg
     6661 tattgatcga tacggcagga cgtctgcata tcgatgaaac attgatggat gagttgaaac
     6721 aaatcaaaga attgacgcaa ccaaatgaga ttttgcttgt tgtcgatgcc atgactggtc
     6781 aagatgccgt caacgtagct gatagcttca accagcaatt aggtattaca ggggtcgtca
     6841 tcactaaact tgatggggat actcgaggcg gggcagcact atcgatccgc tcagtgacag
     6901 gcgcaccaat caaattcatc ggttctggtg aaaaattgac tgacttagaa gtattccacc
     6961 cagatcgtat ggctagccgt atccttggta tgggggatat gctgacattg attgaaaaag
     7021 cgcagcaaga ctacgacgag aaaaaagcgg aagaacttgc acagaaaatg cgggaaaatt
     7081 cattcgattt caatgacttt attgaacagc ttgatcaagt gatgggtatg ggcccactgg
     7141 aagatctaat caaaatgatc ccaggaatga atcaagtccc tggaatcgaa aacgtcaagg
     7201 tagatccaaa agatgttgaa cgcaaaaaag cgatggttta ttctatgacg ccagcagaaa
     7261 gagcaaatcc tgatctgttg aacccaagcc gtcgtcgccg aattgctgcc ggttctggaa
     7321 acagtgttgt tgaagtgaat cgaatgatca aacaattcaa ggaatcaaga aaaatgatgc
     7381 agcaaatgtc caaaggggat atgaacatcc caggtatgga ccaaatgttt ggttcaggcg
     7441 tcaaaggaaa actaggcaaa atggcgatga atcgaatgat caagaaaaat aagaagaaaa
     7501 agaaaaaacg gaaataagat gaaaaaaaca gcttcggtac agactgaaaa tgtctactgc
     7561 cggagctttt ctatcagcaa tttttattct ttactaatac aattactcta aacgaagagg
     7621 tggcacagat gagatgtccg tggagtgaac aaacagaaag tatgcggcaa tatcatgata
     7681 ctgtctgggg tgttcctgaa tatgatgacc aaaagctgtt ccgaaaattg atgcttgata
     7741 ttaatcaagc cggattgagc tggcagacga ttttaaataa aagtgaagca ttcgatgaag
     7801 catatgatcg attcgatatc aaaaaagtcg caaattatgg agaaacaaaa atcagtgaac
     7861 taatggaaaa caaaggaatt attcgtaacc gaagaaaaat cgaagcagcc gtccaaaacg
     7921 ccaaagcagt cttgaaaatt caagaagaaa tcggcagttt ttcagagtat ctttggtcgt
     7981 tttctaatgg aaaaatcgtt gattcttctt atcattcaca agaagaagtc ccaacaacaa
     8041 gtcctctttc agatgaatta gcaaaagatc taaagaaaag aggatttaaa tttatcggaa
     8101 gcacaacaat ctatgctttt ttagaagctg tcgggatcat caatgatcac cttgacagtt
     8161 gtttccgcaa agaagaggtc gtacgtatcg gcagaaaggg cgaataaagc atgcgaaaaa
     8221 tcatatttta tggtgctata agtttagatg gctatttggc aacgaaaacg gatgatcttc
     8281 aatggctttt tgacactcca acaggtaagc aaactactta tgaagctttt tatgaaacaa
     8341 tcgatacgac catcatgggc agaaaaactt atcaggaagc aaaaaaatat ttggagaccg
     8401 aagcaattta tccagagaaa aagaatttcg ttttttccac ccacgcacaa ttacaattgc
     8461 cagatgctga ggtcgtaaac gaagatcctg ttgcttttct aaaaaatcta caaatgacag
     8521 aaggaaaaga tatctgggtt gtgggtggcg gtcgattgat caaaccgcta ttggaacatc
     8581 aaatgattga tgaatggtat atccagatta cccctgtatt attaggggac gggattcctt
     8641 tattccaaaa aggaacatat gaagcgcgtt ttgaaatgct agacacgaca cgattcggtg
     8701 aatttatcga attgcattat gtacgaaagt agaagtaaat aaaataaaaa aggcaactat
     8761 atgcagaaat gtcctgtaca tagttgcctt ttttattacc gttatgcttt ttactctgtt
     8821 aatttgttag aaaaatctag atacgcatct aaaatcgttt ggaagaaact taatgttcct
     8881 tcaacgatat taccattttc atcaattaga ttcgcaacgt ttccgatata tgcttccggt
     8941 tgctgcaagg ttggcacatt caaaaagaca agtgattggc gtagatggtg gtttgcgcca
     9001 aagccgccga tcccacctgg tgatgcagta acgactaaac ctggtttatg atcccaaaca
     9061 ctgtgtccat aaggtcttga gcctacatct aaagcatttt tcaatactgc tggcacagaa
     9121 cggttgtatt caggagtaac gaaaacgaca ccgtctagtc ccctcacttc ctcacgaaaa
     9181 cgagtccatt cagcaggagc ctgttctgga gtttcgagat cctcattgta aaatggcaga
     9241 tcatcgattt ttacaaatac actttcgtac ccttcaggaa gcaatttcgc aaaagcttct
     9301 gctacttttt tgttgtaaga atcttttctt aaactaccaa taaaaaatcc aatttttttt
     9361 gtcatacgat ttttcctcct tcaaactatc tataataatc ttacaaaaga taagtaactg
     9421 gagcaaataa aaacacttac tgacaaaagc tacatagaag atagattact tatttttaac
     9481 aaaaaagaag tctaaaatct tctattttct aaaaaaaatc gaatttaatc aagctgtaaa
     9541 gaaaaaaagc tttacagggt aaaaaaaaac tgttacacta catcttgtga ataaaacaat
     9601 ggaggtggaa ataaataatg gcagttaaaa tccgtttaaa acgtatgggt tctaaaaaga
     9661 gtccgtttta ccgtatcgtc gtagctgatt cacgttctcc tcgtgatgga cgtttcatcg
     9721 aaactgtagg tacttacaat cctttgaaag accctgcaga agtagtttta aaagaagact
     9781 tagttcttga ctggttatca aaaggtgcac aaccttctga cactgtacgt aacatccttt
     9841 caaaagaagg cgttatgcaa aaacaccacg aagctaaatt ctcaaagaaa taaggtgacc
     9901 ggatatgaaa gatttaagcg acttagtctt aacaatcgtt cgtccgctag tcacttatcc
     9961 tgatcaagtc aagttagacg tcgtagagtc gaaagacttt tacgagtaca atctaacagt
    10021 ggctcctgaa gatattggcc gtatcattgg aaaacaaggg cgtgttgcta aagcaatccg
    10081 cacaatcgtt tacggtgttc ggatcaatgc accgaaaaaa gtgcgtttga atatcatcga
    10141 tgataaggaa aagtaaataa acgtgctgta ctcgaatttt cgagtgcagc ttttttgttt
    10201 tttttattaa ttgaagaaat gattatttgg caaaacaagc aagaaagcgt atccttatag
    10261 gaggagggag tgaacagcca atgagaaaga aaatgttatt tattccattt gctctattgt
    10321 taagcgcttg tagttcggtt actcaagaat cagacaatac acatctatca agcagtatat
    10381 ctgaaactgt taccagtcca gaaaaaagta gtaccaaaac ggcttccacc actgaacaaa
    10441 caatggctac ctcaaataat gagaaaaaga ctgctttaga ccagttgaaa gaacaacagc
    10501 caaatgttcc catgcctcta gatgtaccag tctcttcagg ctatctcaat atcgcagcga
    10561 cacatacaaa gcaaggttat tctattttat attacagaac agatcgtccc ctcgggttga
    10621 atgctgacga attgaatcaa gaaacgccta ttgcaaccta tctatatcaa tatgggtttg
    10681 cttctagtca agaaacaatc caagttttgc agccatttga gatcgacaca aatggtcagc
    10741 aagtggactt aggttctcga atcacagggt atcagcaagg tgcagcaggt tcaagttttc
    10801 ttgaatggca agaaggaaac tggcgtattc gaatccgtgg gaataatata gaaggccaag
    10861 atcccttact attagctaaa gaaatcgtag cttatttgga agaaaacagt ctaccagcac
    10921 ctgaacaatt tggtaaaatt actgttgata tgggtgatac gacgaatcga gccgtagaag
    10981 taagctggca agaaccgaaa aatgcctata cgatcactca tcaagatcct atgtctgcgt
    11041 tgaagatggc agtttctatg aagaggttgt gaaatcagcg cgctgatctg agcccatcat
    11101 ttaactccaa gtcacaggaa gtaatcaaaa atagcacgcc gcatttttgc ataattttgg
    11161 cttaatacct taaaagaaaa ctagaattgc ctcttcacac atgctaaaat aaaacagaca
    11221 aaaaagaaat ggggatataa gttgactgaa tacttgaatg taggaaaaat cgtaaacaca
    11281 caaggtatca aaggcgaagt ccgtgttatc tcaacaacgg atttcccaga agaacgttac
    11341 aaaaaaggaa cggtgctaac actattccaa gacggaaaag cacctgtgga attaacagtc
    11401 aaaagtcatc gcaaacataa aaattttgac ttattgagtt ttgaaggaca tccttctatc
    11461 aatgatgtgg aaaaatatcg ggatggcata ttaaaagtgt caaaagacaa ctctgtagat
    11521 ctagctgaaa atgaattcta ttaccatgag atcatcggtc tgcatgtatt agaagatgga
    11581 actgagttag gtaaagtaaa agaaatcttg tcaccaggtg ccaatgatgt ttgggtcgtt
    11641 caacgtccga aaaaagctga tatattgctt ccatatatcg cttctgtcgt caaagaagtc
    11701 gatttagaag ctggagtggt ccgtgtagaa attccagaag gactgattga cgatgaagat
    11761 tgatgtattg actttatttc caagaatgtt tgaaggtcct atgggggaat ccatcatcgg
    11821 taaggctgta gataaaggat tattagatat caatatctct aacttccgcg attattcaga
    11881 taacaaacat cagacagtag atgattatcc atacggcggt ggagcaggta tgctgctcaa
    11941 agtccagccc atctatgata acatccaagc cattgaaaaa gctgcaccgg atgtcaaaaa
    12001 aagagtaatt cttcttgacc cagctggtaa acgattcgat cagaaaatgg cagaagaatt
    12061 ttcacaagaa gaacacttag tattcatttg tggtcattat gagggatatg atgaacgtat
    12121 ccgcacatta gtgacagatg aagtttcctt aggagattat gtccttactg ggggagaatt
    12181 aggtgcgatg gtcatgatcg atgcaacagt tcggcttttg ccagatgtgt tagggaatca
    12241 gacttcagct caaacggatt cttattcaac cggtttattg gaacatcccc aatatactcg
    12301 cccggcagaa tataaaggga tgaaagtacc agaagtattg acaaatggaa atcataaatt
    12361 gattgaagaa tggcaattaa aagaatcact aagaaggacc tatcaacgtc ggcctgatct
    12421 tttggaaaca cttgaatgga cacctcaaat gacgaaattt ttagaagaaa tcaagaaaga
    12481 agaacaagaa aaaacagcag atgaagccaa gtgacttttt ctaaaatctt ctttacagta
    12541 ggttgataat atgttagtat tttatggtga gtgcaaaagc acttaactat tacgatattc
    12601 cgctgtgaga aaagctcata agaatatttg gaataaggag aagaaaacca tgaatccatt
    12661 aatcgaagaa ttaacaaaag aacaattacg ttctgacatc ccagcgttcc gccctggtga
    12721 cactgtgcgt gttcacgcaa aagttgttga aggtactcgt gaacgtatcc agttatttga
    12781 aggtgttgtt atcaaacgcc gcggtgctgg aatcagcgaa acttatacag tacgtaaagt
    12841 ttctaacgga gtgggcgttg aacgtacatt cccattgcat acaccacgtg ttgcaaaaat
    12901 cgaagtagtt cgctacggta aagtacgtcg tgcaaaattg tactacctac gtgcattaca
    12961 cggaaaagct gctcgtatca aagaaatccg tcgttaatca tcgttatcag actgagaaag
    13021 aacccttggt attcaagggt tctttttttg ttctctgttt ttttatatta acgatttcta
    13081 tttgggacat ttttgattaa taaaaagggg caaaaaaggg gcaacaagtt acagtttttc
    13141 tatatcttct ttcaattttt cttttactcc ctttgtcaca tgcaaataaa tcttttcagt
    13201 gatatcacta ttttcgtgcc caacgcgatc ctgaatagca tacagaggag ttcctagttc
    13261 cgctaatttt gaaatatggg tatgtctaaa aatatgtgaa ctaagttttt tatcaatctt
    13321 catgtcagct ttatgatttc tcaaataggt gttaattgct gttagttgga aaggagttcc
    13381 tttcgttgtt tgaaatagaa attgaccatt cggatttaat tctagaagct cattgtaaat
    13441 agctatcgct ttttttggta aatcaatttc tcgcattcca gcagcagttt ttgtagaatc
    13501 agattttttc atatcagcta ttgaacgttc tctatacatc atcgtcccat ttataactac
    13561 tgatgcatta ttattggtaa tgtgtacatc gtctttactt aatgcgattg cttctccagg
    13621 cctcattcca gtcaaatata gccactggaa aaggagagaa tatcttttat tgtgtgaagt
    13681 tgtgaaattt actagtcttt tatattcatc atcttctaaa aacttatctt taatttttat
    13741 cgtttttgac tctcgtttat aatcaataac tacttcgttg ataggatttt tttctacata
    13801 gccttttttc atagcgtatg aaaaaagaag attcaatttg gatttaatta cactgacgta
    13861 cttatttgac aagtcatctt tatatatcat atcttcaaaa atattattta aatctactgt
    13921 attaatacca gaaacgatgt aagtctcagg tatctttttc tttatagtgt ttaagatatt
    13981 attcgtagga taatatgtag actccttaac ttgtcttttg taaataacta accattcttc
    14041 gacaagctca tgaaatgtta gatcaggctt ctgaagtgtt ttttcgttta attttatatc
    14101 aattttttta tttaactcta gcaatgccac tttctgagtt tctcgagatt tatttttata
    14161 agtaatgctc actttttttc tttttctagt ttttggatca atatatcgct cattatattt
    14221 atatactttt tttccgtttt tatcagtttt ggtttcaatc cacattgttt tcatctccta
    14281 tttgttgcta taataggaat ggataagtaa gccaattata gcaggtttat ttttcatcac
    14341 gtccacaaac tttggcgagg gagggggcgt gtttttttgt ttagtagcta cctttccatg
    14401 aagtatctgc gaagaacact tcaaatgttg ttccatcatt tttcacttca aaatatgctg
    14461 ttccttgtgc ggatttaccc ggttggatgg tttccgaaaa gtaatctcta ctagctaaat
    14521 cagatttaat atctgatgaa tcataaaatt ctaaataact tgaacttgca tcaaaaggtg
    14581 tagtccctgt gttttctaca agaaaatcta cctttgcgta aaataggcct tctggtttat
    14641 agaaatcatc accgtaatct tttgatacag actggacagt tacgttaata gagttaccat
    14701 tttcagaagt aaaaggaact gcttctccta tactatgtgc aactgatgcc gtctggggtt
    14761 ctgaagacga agttgtagtt gtagttgtag tagagcttga atctttggat tttccatctt
    14821 tatctaagaa agttcgttga ttataatata caacctgttc agtttttgga ctaacccaaa
    14881 ttaacattgt ttcatcatca gaatcagttg aataagtata ttggagcatc tctaattttt
    14941 ttatatcttt tgcactattt ccatgttcaa ttaattcaga gtgctcttta taataatctc
    15001 cttgtgcatc ctttgaaata gatacaatta attcatcccg tgtgatagca ttgtctaatt
    15061 ctttgtaaac aaaatcattt gtccatttac gtaacggtga accaagtgtc tgataaactt
    15121 gagttaaatc atcaccaact tttattttat gaacgtctga tgaagttagt ttttttgttt
    15181 catattcaga tgttgatgaa acagtatttt ctttagtgct agcagaatta ggactagtgg
    15241 agcatccgct taataccaag cctaaaaaaa gcaacccaaa aacaattttt ttcattttta
    15301 attcctcact tctatgatat gatttttttg taggatctta gaaataaggt ttttagtccg
    15361 tgttgcagca cggacttttt ttacattata taagaaagcg ataagcgctt tctggaagtc
    15421 cgtaaagatt ctttaattcc tcaattcgtt taggatattg atcattgtct tctttgtata
    15481 gagaaacaat gagattagca gcaaagcaat tagcttcgct ttcagatttg cttctagatg
    15541 ttcttgttga tacatagtaa ctggataagc cacgatgaaa aatagcgtga cctaattcgt
    15601 gagcgcaaat gtagaatctt tcctcagagt ctctcagttc atcatttaag aagattattg
    15661 cacgacctct aatttcttga aactgtcctt tgggattctc gataaaagga acgtattgaa
    15721 ttttaatgcc catcttttca caaatataaa aaggattagc ggactggtat ttccgcttca
    15781 actcctcgac taaattaatc gtatccatct ccataagctc acatcttttt gcctttttct
    15841 ttgtcttctt tcacaatatc ccagaaggtt ccaataagga tatcttttac gcgttggacc
    15901 tgttcaggcg tcagtgtttc tccgccataa gacatattga catttgaatc tagtaattta
    15961 tcaagttcaa ctacttcatc ttttgttgcc cagtcaggaa cttggtttct accgagtaaa
    16021 taatcagtag ttacattaaa ataatctgct acaagttgta gtcttttagt actaggagtt
    16081 ctatttttcc attgataaat cgtgttttta ggtatattta attcttcttc caattgagtt
    16141 atacttatgc ctcttttatg agctaattcc tttattctat ctagtaaatt cattttcgca
    16201 cctcacaagc tacgaaaaca ctaataaaaa aattagttaa aggtgttgac aactaataaa
    16261 agtgttagta tactgttttc gtaagctaaa atattagcta aaaagacttg ataactaata
    16321 aacacttcac ggtcggcaaa ctctgaaatg taaattacta ggcatttctg tgt
//
LOCUS       DARDHA010000052        16372 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000052 DARDHA010000000
VERSION     DARDHA010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.052"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="SDA10_001357"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="SDA10_001357"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEG4240275.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="SDA10_001358"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="SDA10_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEG4240276.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="SDA10_001359"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="SDA10_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEG4240277.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="SDA10_001360"
     CDS             2857..3732
                     /locus_tag="SDA10_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEG4240278.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="SDA10_001361"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="SDA10_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEG4240279.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="SDA10_001362"
     CDS             4629..5063
                     /locus_tag="SDA10_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEG4240280.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="SDA10_001363"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="SDA10_001363"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEG4240281.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="SDA10_001364"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="SDA10_001364"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEG4240282.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="SDA10_001365"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="SDA10_001365"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEG4240283.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="SDA10_001366"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="SDA10_001366"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEG4240284.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="SDA10_001367"
     CDS             13334..14260
                     /locus_tag="SDA10_001367"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEG4240285.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="SDA10_001368"
     CDS             14442..15335
                     /locus_tag="SDA10_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEG4240286.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="SDA10_001369"
     CDS             15469..15594
                     /locus_tag="SDA10_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4240287.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="SDA10_001370"
     CDS             complement(15728..16360)
                     /locus_tag="SDA10_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240288.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT         5815 a         2791 c         3492 g         4274 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aa
//
LOCUS       DARDHA010000053        16280 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000053 DARDHA010000000
VERSION     DARDHA010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.053"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="SDA10_001371"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="SDA10_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HEG4240289.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="SDA10_001372"
     CDS             complement(2020..2922)
                     /locus_tag="SDA10_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG4240290.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="SDA10_001373"
     CDS             complement(2919..3611)
                     /locus_tag="SDA10_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEG4240291.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="SDA10_001374"
     CDS             complement(3654..4295)
                     /locus_tag="SDA10_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG4240292.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4602)
                     /locus_tag="SDA10_001375"
     CDS             complement(4333..4602)
                     /locus_tag="SDA10_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240293.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            complement(4575..5264)
                     /locus_tag="SDA10_001376"
     CDS             complement(4575..5264)
                     /locus_tag="SDA10_001376"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEG4240294.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="SDA10_001377"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="SDA10_001377"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEG4240295.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="SDA10_001378"
     CDS             complement(5984..6916)
                     /locus_tag="SDA10_001378"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEG4240296.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="SDA10_001379"
     CDS             complement(6913..7689)
                     /locus_tag="SDA10_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEG4240297.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="SDA10_001380"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="SDA10_001380"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEG4240298.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="SDA10_001381"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="SDA10_001381"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEG4240299.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="SDA10_001382"
     CDS             complement(12014..13294)
                     /locus_tag="SDA10_001382"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEG4240300.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="SDA10_001383"
     CDS             complement(13313..14239)
                     /locus_tag="SDA10_001383"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEG4240301.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="SDA10_001384"
     CDS             complement(14252..15535)
                     /locus_tag="SDA10_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEG4240302.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="SDA10_001385"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="SDA10_001385"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEG4240303.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
BASE COUNT         4518 a         3702 c         2907 g         5153 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttcaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta
//
LOCUS       DARDHA010000054        16056 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000054 DARDHA010000000
VERSION     DARDHA010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.054"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(45..1421)
                     /gene="trmFO"
                     /locus_tag="SDA10_001386"
     CDS             complement(45..1421)
                     /gene="trmFO"
                     /locus_tag="SDA10_001386"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEG4240304.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(1390..3468)
                     /gene="topA"
                     /locus_tag="SDA10_001387"
     CDS             complement(1390..3468)
                     /gene="topA"
                     /locus_tag="SDA10_001387"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEG4240305.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(3590..4447)
                     /gene="dprA"
                     /locus_tag="SDA10_001388"
     CDS             complement(3590..4447)
                     /gene="dprA"
                     /locus_tag="SDA10_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEG4240306.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(4503..5270)
                     /locus_tag="SDA10_001389"
     CDS             complement(4503..5270)
                     /locus_tag="SDA10_001389"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEG4240307.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(5263..6123)
                     /gene="ylqF"
                     /locus_tag="SDA10_001390"
     CDS             complement(5263..6123)
                     /gene="ylqF"
                     /locus_tag="SDA10_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEG4240308.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            6383..6781
                     /locus_tag="SDA10_001391"
     CDS             6383..6781
                     /locus_tag="SDA10_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG4240309.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            7060..7560
                     /locus_tag="SDA10_001392"
     CDS             7060..7560
                     /locus_tag="SDA10_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEG4240310.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(7643..8194)
                     /gene="lepB"
                     /locus_tag="SDA10_001393"
     CDS             complement(7643..8194)
                     /gene="lepB"
                     /locus_tag="SDA10_001393"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG4240311.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(8346..8573)
                     /locus_tag="SDA10_001394"
     CDS             complement(8346..8573)
                     /locus_tag="SDA10_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEG4240312.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(8570..9088)
                     /gene="msrA"
                     /locus_tag="SDA10_001395"
     CDS             complement(8570..9088)
                     /gene="msrA"
                     /locus_tag="SDA10_001395"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG4240313.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(9108..9821)
                     /locus_tag="SDA10_001396"
     CDS             complement(9108..9821)
                     /locus_tag="SDA10_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEG4240314.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(9824..10720)
                     /locus_tag="SDA10_001397"
     CDS             complement(9824..10720)
                     /locus_tag="SDA10_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG4240315.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(10878..11720)
                     /locus_tag="SDA10_001398"
     CDS             complement(10878..11720)
                     /locus_tag="SDA10_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG4240316.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            11852..12505
                     /locus_tag="SDA10_001399"
     CDS             11852..12505
                     /locus_tag="SDA10_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEG4240317.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(12568..13095)
                     /locus_tag="SDA10_001400"
     CDS             complement(12568..13095)
                     /locus_tag="SDA10_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG4240318.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(13115..14062)
                     /locus_tag="SDA10_001401"
     CDS             complement(13115..14062)
                     /locus_tag="SDA10_001401"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEG4240319.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(14074..15960)
                     /locus_tag="SDA10_001402"
     CDS             complement(14074..15960)
                     /locus_tag="SDA10_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG4240320.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
BASE COUNT         4231 a         3324 c         2810 g         5691 t
ORIGIN      
        1 aaaaagagcg agactatccg tctcaccctc ttcatggagt ttccttactc atttgggatt
       61 gcagtttttt ctaattctgt ttttattgcc tcattagctt ttaaagctcg ttgtgcaatt
      121 gcttcatatc ttgctttttt gtctcgaatg cgctctggca gttcaggaaa cagaccatag
      181 ttggcattca tcggttggaa atgttttcct tccgcatgag taatgtagta agccatactt
      241 ccaatagctg tttcttgtgg aaactcgact ggttccattt cttttgctaa gcgtgcggcg
      301 ttgattccag caagtagtcc gctcgctgca ctttcaacat agccttctac accagtcatc
      361 tgacctgcaa agaacagatc ttcgcgtttc gttgattggt aagtcggttt cagcaattct
      421 ggcgaattca taaagctgtt tcggtgcatc acaccatagc gaacgaattc agcattttct
      481 aacccaggaa tcatccggaa aacacgtttt tgctcgcccc atttcaagtg agtctgaaag
      541 cccacgatgt tgtataaaga agctgcagca ttgtcttgac gcagctggat aacagcataa
      601 ggacgtttgc cagttttagg atcttccaat ccaactggtt tcataggtcc gaaaagcatc
      661 gttttgaatc ctcgctgtgc cattacttca attggcatac atccttcaaa atatttttct
      721 ttctcaaaag atttaagttc tgccacttcg gcattgatta gtgcctcatg gaaagctttg
      781 aattcctctt ctgtcatcgg acagttcaag taagctgctt ctcctttgtc atagcgggac
      841 ttcaaatata ctttatccat atcgatcgta gctttgtcta cgattggtgc agctgcatca
      901 tagaaataaa aaccatctga accattgaat gctttgatgc tttctgctaa tggatgagaa
      961 gtcaaaggtc ctgttgctac tacggtgatt ccttccggta tttcagtaat ttcttcgttt
     1021 atcactgtta ctaatggatg ctctttgact tttcgggtaa ttgtttcaga aaaagaatcg
     1081 cggtcaacag ctaaggcacc accagctggc actgctgttt catctgccga agtgattact
     1141 accgaattca atcggcgcat ttcttctttc aataacccta ctgcattagc caaactgttt
     1201 cctcttaatg aattggaaca tactagttca gcaaaattac ttgtgtgatg cgcctcagtc
     1261 gatttgactg gacgcatttc atataaacgg acagggactc ctgcttgtgc gatctgccaa
     1321 gcagcttcac ttccggcaag ccctgctcca acaacattta ccattttcaa tgaaagaacc
     1381 tcttttctat tatttttgga tgtcttcttc gtaatctcca tttacacaga ctacttgttt
     1441 accgcctttg actttcttct cgaccagata tccgtcacat tttggacaat ttctgcccac
     1501 tggtttatcc caagaagtga attcacattc tgggaaacgg gaacaaccat agaatagtct
     1561 gtttttctta gattttcgtt caatgacttg tccttcatga catactgggc aagtcacacc
     1621 gatttctttt acgattggtt tcgtattccg acaatcagga aaattactgc aagcataaaa
     1681 cttcccatat ttccctaatt ttatcaccat cggatgtcca cagacatcac aatcgaatcc
     1741 tgctggttca tctttgattt gaattttttc gattttttct tccgcattcg tcagttcttt
     1801 ttcaaacggt ttatagaaac gatcaaccac tttgacccac gcttccgtgc cttcttcaat
     1861 tttatccaaa tctccttcca tttctgctgt gaaatggata tcgacgattt gagggaagaa
     1921 ttcacagatt aatgaattga caatttctcc tagttcagtc ggctcaaatc ttttttgtgt
     1981 caattttaca tagtagcgtc tttggatcgt atcaagtgtt ggtgcataag tggaaggtcg
     2041 ccctaccccg ttttcttcta gtgcacgaat caacgtagcc tcactaaaac gtgctggtgg
     2101 ttgagtaaaa tgttgttttg gttcaatatc aacagattgg actacatcgc cttcttcaag
     2161 ttctggaaga atattttctt tctcttcttt gccgtcatct ctaccttcca catacacttg
     2221 catgaatcct ttgaatttga tttttgaacc gttcgcaatg aagatgacac cattttgttc
     2281 taatgtcact ttcattgtat ctaaaatagc tggtgtcatc tgactagcca caaaacgtga
     2341 ccagatcaat gtataaagtt tcaattgatc tttatctaaa tattgtttga gttcatctgg
     2401 tgtacgaagc acactcgttg gacgcacagc ttcatgggca tcttgtgcgc cttgagcatt
     2461 tttgatcttt cggtgatcat gtctagaaaa ttcttttcca tacgtatctt caataaatgc
     2521 tgcggcctct gcttttgctg tatcggcaat ccgggtagaa tcagtacgca tgtatgtaat
     2581 caagccgact gttccttgtt tacccaaagt gattccttca tataattgct gtgctaccat
     2641 catggttttt cttgttcgaa agtttaactt acgggcagct tcttgttgca ggctacttgt
     2701 agtgaaagga ttcgcagggt ttcttttgcg ctctttcttt tctacttttt taacttcgaa
     2761 gtctgttcca tcaattctag cagtgacttc ttttacactt tgtgcatctg gtaatttctt
     2821 tttcttaccg tcgattcccc agaaatttgc tttgaatttc ttgcgagctt ttttaaaatt
     2881 accgtcgatg ctccaatatt cttctggaac aaattctcgg atctcttttt cacggtcaat
     2941 aataattttt aaagcgacag attggacacg tcctgcactt aaccctttct tgaccttacg
     3001 ccagagaata ggactgatcg aataaccaac gattctgtct aaggtacgac gagcttgctg
     3061 tgcatctact aaatctacat cgatcgttct tggttcctta aaagctgctt tgactgcgtc
     3121 ttttgtgatt tcattgaaga ccacccgatt tttttctttc agatccaacc caagtaggta
     3181 cgctaaatgc caagcaatcg cttctccttc tctatccggg tcacttgcca agtaaacttt
     3241 ttgagctttt tttgctgcgg ctttcaggct tttgatgaca tcgccttttc cgcgtataga
     3301 aatataatgg ggttcataat tgttttcaaa atcgataccc attttacttt tcggtaaatc
     3361 tcttatatgg ccaacactgg ccattacttt atagtttcgt ccaagatatt tctcaattgt
     3421 cttggctttt gcaggtgatt ctacaatcac taaatattta tacgccatta ctgaatggac
     3481 tcctttccat tgtcgtgttc ctactattaa aagaggattc atacaaatcg tagctaatca
     3541 tatccattca gaaaacgttt gtcaagaaaa gcaaatgaaa agtatggtgt tattctttaa
     3601 attctgccaa gatatcttca cccgaaaccg tgcaaactgc accgtcttgg atcaaatgat
     3661 gacatccggt agaatgttct gttaaaatat ttcctggaac agaaaaaact gttcttccaa
     3721 gatccaatgc ttgctgtgct gttatcagag agccgctttt tttcttggct tcaaagacac
     3781 atgttccttt agacaaagct gcgataatcc gatttctcat tggaaaacaa tgctttttcg
     3841 ggccggattg aggaggatat tcactcaaaa gaagatgatc atttgcaatc acctcttgca
     3901 agtcacgatt ttcagcagga tagacttgct ctatccctgt tccaatgact gcgattgtcc
     3961 gtccaccatg agagattgct gcttggtgac cgtatgtatc tatccctttc gcgcagccac
     4021 tgacgatcac atatccagct tctatcactg gttttagtaa ataataaact gtttttttgg
     4081 ctaaagcaga agtgtgtctt ccaccaacaa cgcttaacat gttacaagaa agtaaagata
     4141 gatcaccatt ataaaacagt acaattggcg gataggtcaa gtgaagcagc tgagggggat
     4201 atttgggatc aaagcaagtg ataaacgact gtttcgattg gattttgccc cagttagcgt
     4261 gaatttcctg ccaacttttg cgaaacagct cccgatgttt ggttatttta cctatcttaa
     4321 tgatgtcttc aaatccgatt tctttataag gatactgcat catccagtta gcaagtgccc
     4381 attttccttg actactgatt ccttttgtat aagcaagttt caataaattt tcttctattt
     4441 gatacatcat aaaaccgcct ttcgtctctc tttctaataa gatacgtaaa agcggctgtt
     4501 ttttattgta aatatttttt tacaggtgca aaacttttac gatggattgg cgtaattcct
     4561 tgtcgttcaa gtccttctaa gtgttccttt gttccataac ctgcattatg cgagaaacca
     4621 tatccaggat aaagcacgtc atattctttc atcaattggt ctcgatatac tttagcaatg
     4681 atacttgcag cagcaatcga cacagatcgt gcatcccctt tgattatttt ttcctgctga
     4741 ataggcaaat ccaaggtcat cgcatcgatc aacaaatagc ttggtacaat ggacaatttt
     4801 tctactgctt ggatcatcgc taatttactt gcttcataaa tattcacatc atcgattgtc
     4861 tgctgatcaa cgatcccgat cccgattgcc agggcttttt cttgaacttg ttgataaaga
     4921 agttctcgct ttttctctga taattgtttg gagtcattca gccctaatat gatctcattt
     4981 ttcggcaaga taactgcagc tgccacgaca ggtccagcta atggtcctct tcctacttca
     5041 tcaatgccgg caatccattg gtgaccttgt gcgtaccctt gcttttcaaa gcaaaacatt
     5101 tcttcatatt tttccttaat taatgcttcc tttttcaatc gtcgttcaaa agaagccagt
     5161 gcttgctgca cgcctttgcg tggatcattt cttaatgatt ctagtgtagg atcatctatt
     5221 ctctgtatgc ttttcaatcg ttctttgata ctttggatgg attcagtcat cacttttttg
     5281 ctccatttct tcgcaacgat ccagtgtgta tcgtccaagt tttccttgac gaatatcatg
     5341 cacgactaat tcgctgccac gatcatagtc ttctttatac ccacgttttg ctgtaatatc
     5401 catcagtaat tccggtgcca atttttcttc ttgctcgatt gataaaccat aacgatcgct
     5461 gattcttcct gaataatatc gggcaaaaaa gtctaatccg taaatcgcta aatcatctaa
     5521 atggacaagc tggtctttga tcgcgcctgt cagcgctagt tttttgccga tttcttcatc
     5581 ttcaaatttg ggccataaaa tccctggtgt gtctaacaat tccaactcat tacctaaacg
     5641 aagccactgc tgaccttttg ttacgccagg cttatttcct gtctgtgcaa ttttcttgcc
     5701 tactagtcga ttcatcaacg tagatttccc aacatttgga atacctaaga ccattgcccg
     5761 aatagcgcga ggcttgatcc cccgagcagc atctcttgcc cgtttttctt tcaatgcttc
     5821 tttagccgca ggtactagac ctttaattcc tttactttct tgtgccgtga tagctaatgc
     5881 aagatggcct tgtttacgaa aatattcttg ccacagcttt gtctgttgtg gatctgccag
     5941 atccgcttta ttcaataaaa caaggcgagg tttttgctga atgatctgat ctagcattgg
     6001 attacgagaa gataaaggta atcgtgcgtc taccaattca aatacgatgt ccacatattt
     6061 cattttttca gaaacttctc gtcgggcttt tgccatatgt cccggaaacc attggatggt
     6121 cattgttctc tccattcctt tcataccgga atattccccg gctttacttg tttttttctt
     6181 tttcaatata aattcacttt tttattataa agaaattcca tctattagtt aagtgaaagt
     6241 gaaccagtcc tcattctatc acatcttcac ttttttgatg atgcaaattt ttattttggt
     6301 ttctatatat gaatctaaat ttttgacagc gtttcctaaa taagagatga tagacgaaca
     6361 aaacagaaag gagacgaaac aaatgaatag tatggttttt gttaattttc ctgttgctga
     6421 tgtcaatcgt tccgttgctt tttatgaaaa gctgggtttt aaaaaaaatg aagaattttc
     6481 tactgaagaa gctggttcaa tggtatggga tgacactttt tggataatgc ttctctctca
     6541 tgatttttat aaaaaattta taaaagataa agaaatagct gataataaaa aaacaagtgg
     6601 tgctttgatt tcgtttagtg ttgaaaacgc ggatgaagta aaaaaatttg ctcaaattgc
     6661 aaaagaaaat ggtggtactt tccacgaagt agatatggga atgcctgaag aagaaatgtt
     6721 ttcattagaa gtccaagatc tagacggaaa tacattagaa cccgtatgga tgaagatgta
     6781 agcagataac taatctgacg ttttttataa caaaaaaaca aaggcggatg atacgataag
     6841 tattctccgc ctctattttt tttattcgta caatctcttt tttacagttt ataaaagcag
     6901 tcttctcacc ctttttttaa ttgttagatg ccagtgtatg taaatcacca atgtgtcatg
     6961 acacattggt gattttatgg tatcctttta ttattaactg acaaaggaga gaatattttg
     7021 aacactttga ttgcaaaaga cgtttttaga ttgcccgaaa tgaatgcaag attgttgtcg
     7081 acctctgctc ttttattagc ccttgatgtc atcttataca agatagcgat tggtcctgct
     7141 tattttcaaa tcacattcgg atttttaagt atggcgctta tgggttatct atatggaccg
     7201 atctgggctg gtttgcttga aatgttgtcg aacactttaa atttcacgat atttggttca
     7261 ggaacgtttc aagctgcttt tcttatcacg gctttccttg gtggatttat taatggctta
     7321 tttttatatc ataaaaagat aaacctgcct tccgtcatcg ctgcccaatt attgattatg
     7381 atcccagtca gcctttttat caattcttgg ctgatgtctg ttttgtttgg tgcagatttc
     7441 aaagtaattt ttctaggtag attcatcagg aatatgatcc aaatccctat tcaaataatg
     7501 gttgtttatt catttatcaa aactttagag gctagaaaag tgtttaaacg agctgtttga
     7561 ttttacgtac taaggtagta aactgatttt ggattaaaca ataaagcgtg cagtctcaac
     7621 agactgcacg ctttattgtt tctcactgga tccattttat ttcatcaagt ggataataga
     7681 caaatcttgc tttacctaga atatcagttt tattgatcgt gccaaacgaa cgactatctt
     7741 ttgaaactct tcgattatct ccaagtacaa aataattatc ttccggaagc ttggattgtc
     7801 ccgttagctc ttgtagtgta aagtccgtcg tataggatgc atttgcatga tctgatttca
     7861 ggtttttagt tagataaggt tccttaacta ctttcccatt gatcttcaat tgatcatttt
     7921 ggtagctgac attttctcct ggaagaccga ttactcgttt gatataaatc gtgccatctg
     7981 ctaattgaaa aacaacaata tcaaatcgtc tgatctccga aaatttttcc atcacaacca
     8041 tatcaccttg cttcaacacg ttttccattg aattgccttc cactggtacc gggatgaata
     8101 aaaatccccg caacatgaac gcaaggaata cagaaacaag taagtatttg aatacaagcc
     8161 agaagcgatt aatatagcgt tgtttttttg tcaatctcta tcaatccatc cctctttttt
     8221 tcattctttc tttattataa gaaaaatatc aaaaaaatgc gactagatag ctgaaataaa
     8281 aataattctt tcaattaggc tattggacaa aaaacttttt tgatcataaa gtacaaaact
     8341 atctcttata tgtcatgatt attttctaaa tatttttccc atgcttcgtc aaagatatcc
     8401 atggacggca aatagtcgac attcagttcc agataattag aaatttcttc gtagctagag
     8461 ctgtgcttag gaaattggat gtctttaccg gcttctgtag cgaaaacaga taaagcatca
     8521 tgtgtgccac cgcctcgcag tgtcattaaa taatgaaaaa agcttcgatt cacccttcgt
     8581 tcctccaatt ttcttcgata aaggcagccc ggcgtgcatg gtttctttta aaattttcag
     8641 gatgcttttt ataatactct tgatggtctt cttctgctag ataaaatggt tgggctggtt
     8701 cgatttttgt aacgattggc tgttcgaaac gtccactttc agctaactgt ttcttacttt
     8761 tttcagcaat gatccgctgt tcttctgtca aataaaagat caccgggcga taattgtcac
     8821 cgcgatcctc gaattgtccg aaagcgtcag ttggatcagt ttgctgccag taaagttcta
     8881 ctaattgttc atatgaaatc aatgttggat catagataat ctccactgct tctgtatgtc
     8941 cagtcgtttg gctttttact tgttcataag tcggatctgg tacatgccct ccagtatatc
     9001 ctgacatcac tgtatggata ccaggaagcg tgtcgaaagg ctggatcata caccagaagc
     9061 agcctcctgc aaatattgct cgttccatta tgattccccc tctaatacta ctcatccgat
     9121 gattttttat tttcttttgt cttgctttca gtactgctgg tgctagtttt gttggtattt
     9181 gagttgtttg aagcatcaga ggtatctttt ggcaaatata gactaaaccg aatgtcatca
     9241 tttactaagt cgattttatc tgctttaata tacatcccgt tttgcattcg gaattgatcc
     9301 aacctaagca tgatagtcag atcatctgta ttgacttcga tccaatctgg taatttatag
     9361 tttctcttga tcatattcat cacatctttg attggcaaac tcaatgttcc gatagaaaga
     9421 cttctagctt ttagttggac atttccgttt tccatcacat acggatcaaa atataaatag
     9481 aaatcgatag gaaaacctag tacttcgaaa gtaccattga gtagtgcttc attttccaaa
     9541 taaaatttat attcaatatc actatctttt tgatactcac taaggaaaaa gctgatgatt
     9601 tgattgactt tttctttatt tgaattgatc gttaagacag gatctccttg tctttccgta
     9661 agagctggta tttctgggag ttctggttcc cgattagcaa aaatacggct gcctaaaaat
     9721 acggtcgaac caagtactac cccaagaagg ataaggaagg cgaatttcca gtaatttgtt
     9781 ttctttgttt tgtttgaaga tacgtcagat cttgttttca tgtttattga tcatcctcct
     9841 tctctaacca ttggttttgt gtctcgatca gctgatctct cactgcattt gccatcaatt
     9901 gataacctaa attatttgga tggaaatgat cttcttcata gagcacatta tttttgatgc
     9961 catttagatt ttctgtcgtg ctagttgttt gatctgttcc ttcttctgtg acacctactt
    10021 cctgattcaa tccttgataa agcaaatcat tgattgggat aaagtagcag ttcggcattt
    10081 tttttgtcac cgtttctgtt cctttgttcc aattgttaac aattgtctgc atgtcagtga
    10141 tctcaggaaa gttcaaataa aatggattgt agatgcctaa aatgtaaata ggtgcggttt
    10201 tattgaggga tcgaaattct tccaatagtt ctgtgacatt ttcctgatag tttttcagtg
    10261 gctttttaaa ggaattgacc gttaacccga ataaattatt ttggataact ttcatcaaat
    10321 cattaccgcc aacagtcagt gtgataaaat cagctgtttt gatattgttt cgaatttctt
    10381 cttttgtttt tactcgcttt aagatctggt cacttcgttc accatttaca ccatagtttt
    10441 ctgtttcaac cgcggtcaaa ttataacggt cttttagatc atttgcgaca aggggaacga
    10501 atcctccttg cttggtttga tctcctactc cttcagtcag cgaatctccg atagctatgt
    10561 agtgaagttt ttctttttta tttggatctt ctctcacagt agttactggt ttcattagtg
    10621 ggccagcttt cggtatagaa gcagctaaac ctgcatagat gatgatcgca attaataatg
    10681 ggaacaaaac gaggatcaaa tgtcgtttcg ttaatttcat tttttcacct cttgtattcg
    10741 attggttcta atgttatcaa aaattttatc aaaaaggtag tctttatgaa aaaagaaaac
    10801 aattattttc ctaattttat aaagaaaaag agaataagct gcaagccttt caggctgagg
    10861 gcttatcctc ttttcgacta atctacataa tacatgattg caaacgcgtc tttaccagta
    10921 tgcgtagcga taaccggatt cgtatgcagt acagggatat gcatgtccgg gaataattct
    10981 tgcaaaccct ctttgaattc ttgcgccaat tcttttgcat ccgcatgtga aataccgatt
    11041 tgtcgcacat ttggcaattc tttcagttca tttttcaaat tattaaacca tttatcgaac
    11101 gttttctttc ctcgcccctt agttacaggg attaattcag aatgggcgaa attcatcacc
    11161 acgcgtatgt tcaaaacact agacagcaaa ccagttgttc ggctgattct tccacctttt
    11221 accaagttat ctagtgtgga tattccgatg tataattttg tattgtcttg tacttttttg
    11281 acttcttcaa ggattttctc tactgtttta ccttcttttg ctaattcagc tgctcgaatt
    11341 acctgaaaag ataatccttg gtcagtggta tcactgtcga taaccgttac tttcgttttt
    11401 gttagattac tggcttgtct tgctgcttct actgttccac tcaagccttt tgtcatatgg
    11461 atcgaaatca cttcactgcc gtcttctcct agctcatcat acatcgagat gaattctcca
    11521 attggtggtt ggcttgtttt gggcagattt tttgctttcg ccatcatttc cataaatctt
    11581 tcgccgtcta gttggtcatc atcgggatat actgttccgt cgatcatgat ggatagcggc
    11641 atgacacgaa tgcctaaagc atcgcgtaca tttttttcca tcgtacatga agaatctgtt
    11701 acgattttta catttgtcat tattttctct ctctctttct ttaacaagat gccttattca
    11761 tgttaaacta catgtaagac aatttctatt tttagtataa cagacaaata atgatttttc
    11821 attaattaca cctagtaaag gagccgtttt catggaaaaa acgaaattta atcgaacgta
    11881 tgaaatatta aatgaagtgt ttaatgcagt cacccatgga attgggactg gattgagtat
    11941 cgcgggacta gtggttcttt tagtaaaagg tgcccacttg cattcctccc tccatattgt
    12001 ctcttatgct atctatggat cgatgatgat tcttttattt ttaacgtcta ctttgtttca
    12061 ttcattgatt tttacaaaag caaaaaaggt gttccaagta ttcgatcata gttcgatttt
    12121 tcttttgatt gcaggaagtt atactccttt ttgtttgctg agtatccgag gatggctagg
    12181 ttggacttta tttgtattaa tctggttgtt tgcgattctt ggcattatat ataaagcgtt
    12241 gacgttgcat aaacgggaga gagtatcaaa agtatctacg atcatctata tttttatggg
    12301 gtggctctgt gtcgtggctg ctgttcctct ttatcactct cttggaatga ctggtgttat
    12361 tttgatggta gcaggcgggc tttcttacac gataggcgca ttcttttata gcttgaattc
    12421 tatccgctat atgcatgttg tttggcattt gtttgttatg ttaggtgcag gttttatgta
    12481 cttctctgtt ttattcttta cttaataaaa aaaataaagc tatggatggg acagctctaa
    12541 ataacagcta gtctatccat agctttttta tatcgtcaat tcgcctgtcc cgctttttac
    12601 aggattctca ttacgctgat acgtttcaaa ttgatgcgga taacgatttt tttcatccat
    12661 ttctccttga ctagtattga tcagtgacca atcagaccag tcaacaggtg gaaaataagc
    12721 atctccatcg aatacatgat ggatgactgt acggtataat atcgtacaaa atggcagaaa
    12781 ttcttgataa acagctgacc ctccagcgat gaaaaagata ccgtctgttt ctttttccag
    12841 agcaagcacg tcttccacac tatgcatgac atgcacgcct tctgctttat aggaagaatc
    12901 tctcgtcaaa acaatcgtct gacgattcgg caacggacgg ctacccatcc cttcgaatgt
    12961 ttttcgtccc atcactagtg tatttgcttc tgtcatttgt ttgaagaatt tcagatcatt
    13021 tggcaaacgc caaggaagac ggtcttcttt tccaatcaat ccgttttcat cctgtgccca
    13081 aattgcagct aacattttct catcctttct gattctaaac agcgatagga gctttgatcg
    13141 ccggatgtgg gtcatatcct tctaattgga tatcttccat ctcaaaatca aatactgact
    13201 gcttttcctg attcaaaact aaagtcggaa atgcgcgtat ttctcggctc aattgttctt
    13261 tcatttgttc gatatgattg atataaagat gagcatctcc taatgtgtga acaaattcgc
    13321 caaccgctaa acctgtttca tgtgcaatta ggtgagtcag caacgcataa cttgcaatgt
    13381 tgaacggaac gcctagaaac acatccccac tgcgttgata caattggcag cttagccgtc
    13441 cctcttgaac ataaaattga aacatcgtat gacaaggagg caaagccatg ctaggaacat
    13501 cttccggatt ccatgcagaa acgatcaatc ttctagaatc aggagtcttt ttgattgctt
    13561 cgatcacttg tttgatttga tcgataaagc caccgtcttt tgtttcccag tgacgccatt
    13621 gatagccgta gatgtgtcca agatcgccgt atgtttcagc aaaggaatca tcggttagga
    13681 tcttctcgca aaaaagcttg ctctgctttt tatattcttc attgaattca ggatctgtca
    13741 aacatctatg gccaaaatca gtcatatctg gtccattata gtctggactt ttgatgtagc
    13801 gttcaaatgc ccattcatcc caaatatggt ttttattttg taatagatag cggatattcg
    13861 tatctccttt taaaaaccaa agcaattcgc ttttgatcaa tccaaaaggt actcgtttcg
    13921 ttgttaacaa aggaaaccct ttttgcagat caaaccgcat ttggtatccg aataagctta
    13981 atgtgcctgt gcctgttctg tcttctttac gatgcccttc ttccaacaat tttcttccta
    14041 agtcaagata cgcttgttcc atcctttttc ctcctaatct tctacccatt cactcaagta
    14101 ttcccaccgt gccatttttt cttcaagttg tgcttctgtt tcagatacgt cgttttgcaa
    14161 ttcttgtaat cgagtgaaat tatctccttg atgattcatc tcttcttgga gcaatgagat
    14221 tttttcttca agttctgcaa tctcatcttc aatcgtttcc cattcttttt gttccatgta
    14281 agataatttc ttcttttcag caggttcctt gctctgtatt tttggtttcg ctttttcagc
    14341 aggctgctgg gtctcttttt gcttcagtaa ataactggac atgtttccgt aataaagttc
    14401 ttgttttccg tttccttgga atactaataa cttatctgct accttgtcca agaaataacg
    14461 gtcatgggaa acagtgatca cagcacctgg aaaacttcta aaataatctt ccaaaatcgt
    14521 caatgttgct atgtccaaat cattggtcgg ttcatccaat aataaaacat taggttgctg
    14581 aatcagcagt ttcaacaaaa ataaccggcg tttttctccg cctgaaagtt tgcctatcag
    14641 tgtcccatgc ataaatcttg gaaataaaaa tcgttcaagt aattctgcta ctccgatttg
    14701 actgccatct gctgtttttg cttcttcagc agcttcttgt aagtaagcaa tcatccgttt
    14761 atctggtgcc atttcttcat tttcttgtgt ataatacgca agatgaaccg tttcaccgat
    14821 agacattgta ccgctatcca ggggaattct gccagctaga atattaagca atgtcgattt
    14881 tcccgcacca tttttaccag taatacctag tctttcccga gattgaatca ataaatctaa
    14941 atgttctagc agtgtttggt catttatacg gtaactgcca tctttgattt ccagtacttt
    15001 tttccctaaa cgttgtgtcg ctaggttcag ctctaaatca tcttcttggt ttacttggta
    15061 caagttctcc tttagatctt cgaaacggtt gatccgtgct tgctgcttgg tgccacgtgc
    15121 ctgtacgcca gccctcatcc aagctaattc ctgtttataa agccgcttgc gcttttcttc
    15181 ttgttctact tctacacgat cacgttctgc tttttctaag acgtaagctt cataatttcc
    15241 tttatactcg tataattttc caaatgaaag ttcgaagatt cggtttgcta ctctatctag
    15301 aaaataacga tcgtgggtaa ccatcaatag tgcgccacgg tattgtttca aatagttttc
    15361 caaccactcg atcgcttcat aatctaaatg gtttgttggt tcgtctagta atagtaaatc
    15421 gggttcatca atcaacactt gcgccagact aactcgtttt ttctgaccac ctgataaagt
    15481 actgattttt ttatctaatt cactgattcc taatttttgc aaaataatct ttgcgttggt
    15541 atctgtagtc caagcatctt ctttattcat ttgttcttct gctttagcat aacgtttttg
    15601 cacatcaggg tcttctccgt tttcccctag ttcgatcaat gcttcctcat atgctcgaac
    15661 agtttggatc aaggggctgt ttccttgaaa gactgcttgg agaacagtta attcatcgga
    15721 aaattcagct gcttgagaaa gataaccaat acggtaatca gttggataaa acactgtttg
    15781 ccgatcaccg tcgctgctat cgataccggc aataatattt aagagacttg ttttacccgt
    15841 cccgttcgtc ccaatcaacc cgatacggtc tttttcgtgg accaaaaaag aaatctggtc
    15901 aaacaaatct ttttcaccat aagttttttt gaggtcagtt acttttaatt ctttcaacat
    15961 gttccctcct tatcttggat attgtatcat aaaaatagtg aacttcagga aaattttttt
    16021 ggtatttgtt ctttttgtgt tctcaaaaaa agaaca
//
LOCUS       DARDHA010000055        15898 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000055 DARDHA010000000
VERSION     DARDHA010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.055"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(59..187)
                     /locus_tag="SDA10_001403"
     CDS             complement(59..187)
                     /locus_tag="SDA10_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEG4240321.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="SDA10_001404"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="SDA10_001404"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEG4240322.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="SDA10_001405"
     CDS             complement(1674..2729)
                     /locus_tag="SDA10_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEG4240323.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="SDA10_001406"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="SDA10_001406"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEG4240324.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="SDA10_001407"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="SDA10_001407"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEG4240325.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="SDA10_001408"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="SDA10_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEG4240326.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="SDA10_001409"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="SDA10_001409"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEG4240327.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="SDA10_001410"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="SDA10_001410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEG4240328.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="SDA10_001411"
     CDS             10491..12050
                     /locus_tag="SDA10_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEG4240329.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="SDA10_001412"
     CDS             complement(12161..14092)
                     /locus_tag="SDA10_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4240330.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="SDA10_001413"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="SDA10_001413"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEG4240331.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="SDA10_001414"
     CDS             complement(15021..15773)
                     /locus_tag="SDA10_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG4240332.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
BASE COUNT         4105 a         3487 c         2908 g         5398 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcctgc ttttttgaag taagcgtcat aataacccgc gcttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tcagagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 aattggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcctcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgtttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttccgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgatagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcgattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg cgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ctccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggaa attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat gtcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt taccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccggtcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatacgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaatgtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtgggg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagaggc tgggaaaacc cagcctcttc ttttttattt ctatgcttct gctactgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgccg tgaggagcca
    12301 gtaatttgat tccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcaactc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcaaagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccagcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagtaat tttttcccat acggaatgat ttcttctaca gattggaagg ttgtgttgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaac ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagctggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cctaagccat tgatctcagt ttcagtgtct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg gatccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattcaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctgcgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctacta gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcag aaagactg
//
LOCUS       DARDHA010000056        15766 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000056 DARDHA010000000
VERSION     DARDHA010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.056"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            128..1465
                     /locus_tag="SDA10_001415"
     CDS             128..1465
                     /locus_tag="SDA10_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEG4240333.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1513..3642
                     /locus_tag="SDA10_001416"
     CDS             1513..3642
                     /locus_tag="SDA10_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEG4240334.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3617..4306
                     /locus_tag="SDA10_001417"
     CDS             3617..4306
                     /locus_tag="SDA10_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240335.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4714..5400)
                     /locus_tag="SDA10_001418"
                     /pseudo
     CDS             complement(4714..5400)
                     /locus_tag="SDA10_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5536..5730)
                     /locus_tag="SDA10_001419"
     CDS             complement(5536..5730)
                     /locus_tag="SDA10_001419"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240336.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5780..6220)
                     /locus_tag="SDA10_001420"
     CDS             complement(5780..6220)
                     /locus_tag="SDA10_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEG4240337.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6224..7069)
                     /locus_tag="SDA10_001421"
     CDS             complement(6224..7069)
                     /locus_tag="SDA10_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEG4240338.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7218..8636
                     /gene="pepV"
                     /locus_tag="SDA10_001422"
     CDS             7218..8636
                     /gene="pepV"
                     /locus_tag="SDA10_001422"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEG4240339.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8693..9640)
                     /locus_tag="SDA10_001423"
     CDS             complement(8693..9640)
                     /locus_tag="SDA10_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG4240340.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9863..10213)
                     /locus_tag="SDA10_001424"
     CDS             complement(9863..10213)
                     /locus_tag="SDA10_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEG4240341.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10413..11492
                     /gene="pepA"
                     /locus_tag="SDA10_001425"
     CDS             10413..11492
                     /gene="pepA"
                     /locus_tag="SDA10_001425"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEG4240342.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11636..11956
                     /locus_tag="SDA10_001426"
     CDS             11636..11956
                     /locus_tag="SDA10_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG4240343.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11978..12442
                     /locus_tag="SDA10_001427"
     CDS             11978..12442
                     /locus_tag="SDA10_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG4240344.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12647..13252
                     /locus_tag="SDA10_001428"
     CDS             12647..13252
                     /locus_tag="SDA10_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEG4240345.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13291..14580)
                     /locus_tag="SDA10_001429"
     CDS             complement(13291..14580)
                     /locus_tag="SDA10_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEG4240346.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15008..15487
                     /gene="rlmH"
                     /locus_tag="SDA10_001430"
     CDS             15008..15487
                     /gene="rlmH"
                     /locus_tag="SDA10_001430"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEG4240347.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT         4957 a         2917 c         3228 g         4664 t
ORIGIN      
        1 gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc taactacata
       61 agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa atggacggag
      121 gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct ttttcatggt
      181 ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg ctactgtatg
      241 gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg aagggcaagg
      301 agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg ccaaatcgat
      361 cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata gcggaatcgc
      421 agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa tcacgatgag
      481 cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg gaccagtgat
      541 ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag taccggttgc
      601 actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg tcaatttcaa
      661 acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata cttataatga
      721 ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac taacggtcgc
      781 taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac cagctgaagg
      841 tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag gggtcgttct
      901 tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat tgctgtttac
      961 tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat tatttacaga
     1021 tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga tgttcaataa
     1081 ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga taccgcaatc
     1141 accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt tccgcggtcc
     1201 aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg ttaatccaac
     1261 gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc ctcagcattt
     1321 agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa cttctcgaga
     1381 atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat gcctgatcac
     1441 gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat tagatgaatg
     1501 gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg tacacatacg
     1561 aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc agtgaaaaca
     1621 acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca aaattgtttg
     1681 ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac cacacaagcg
     1741 actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat ggcaaaaggt
     1801 ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga tttagggaat
     1861 aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa tgtagaccgt
     1921 gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt ttcttctatg
     1981 gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc atcggtaaaa
     2041 tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac aagaagaact
     2101 cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc aacctctaca
     2161 gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg cggagacggc
     2221 ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct ttccggtcca
     2281 gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt ttattttgaa
     2341 gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc aatcgattat
     2401 tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt tctaggtgtg
     2461 gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg tccacgttct
     2521 gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat caaaggtcct
     2581 aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa ggtagtgatg
     2641 gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct tggattagtg
     2701 caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat ccaagcatta
     2761 gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga aaaatcctat
     2821 gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga gaaagatcag
     2881 atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt tagtaataaa
     2941 atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat tggtgtagca
     3001 cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa agaagatata
     3061 cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac accagaatcg
     3121 atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat cgctactggt
     3181 tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga agcactagag
     3241 cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga aaatacacct
     3301 ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct tcggatcatc
     3361 gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa aacaactgca
     3421 gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta tcaaactgaa
     3481 ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga ttttacagcg
     3541 acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat ggaaccggag
     3601 gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag agttactaaa
     3661 cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc gagatttatt
     3721 ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa tcgattgtgg
     3781 tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag gagaaatttg
     3841 tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg aacgaatcac
     3901 ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt tagaaaaatt
     3961 aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga ttattggaca
     4021 tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa gcgaaaaaat
     4081 tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat tgagcgagat
     4141 agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt atgtattaga
     4201 ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag aagaaataaa
     4261 aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag actgtgacaa
     4321 aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa ctcctcggtc
     4381 acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc cttgtgactc
     4441 ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct tgcatctgcg
     4501 gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct gttcttatta
     4561 cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc agctgaccct
     4621 cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt tctttctcta
     4681 atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt cctgaatatc
     4741 ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa tcatatgtaa
     4801 atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg attgaagcat
     4861 tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca aacgtaacca
     4921 tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt ctcgttttcc
     4981 agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag cttttttatt
     5041 ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag cttgatctta
     5101 cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt cccgcgtata
     5161 acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg actgtggaat
     5221 ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg gtgtacgccc
     5281 gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg ctaactcgga
     5341 ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat tgataaccat
     5401 ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta caaatcgtgt
     5461 gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta gagcctcgaa
     5521 aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac gaccgccgat
     5581 tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat tgtctaagtt
     5641 tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt taacgacata
     5701 catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca atacaagaaa
     5761 aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac gcttggaata
     5821 actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa tcagaaagta
     5881 aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt cccacaattt
     5941 cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg atgctcactt
     6001 tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg gggatgatga
     6061 tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag gctacccgag
     6121 cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga tctgtttcat
     6181 aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc tcgttttcca
     6241 ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt tgtgtcggca
     6301 aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga taaatagctg
     6361 ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc atcccagcta
     6421 aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg gtgtagaagt
     6481 gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca ccgatttcct
     6541 gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa ctcaaccgtt
     6601 gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc aaaagatgat
     6661 tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct tttgattggt
     6721 tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat ccgggaccaa
     6781 tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc gtccaaacta
     6841 caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca tcagttacta
     6901 cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg attgctccgc
     6961 catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta tgtgaaattt
     7021 ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc taaaaacctc
     7081 cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa aggaattgtc
     7141 agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa gacttaaata
     7201 agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca cgtaaagaag
     7261 acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat gactcaaaag
     7321 taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat atgttagctt
     7381 atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg catattgatc
     7441 ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt cctgcaggag
     7501 atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc tttgcacgcg
     7561 gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag atcattaaag
     7621 aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat gaagaaagcg
     7681 gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt ggtttctcac
     7741 cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta gctttacgct
     7801 tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga cttcgcgaaa
     7861 atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat gcagctatcg
     7921 cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga acgattgaag
     7981 cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt gcaagcccac
     8041 aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat gaattcctag
     8101 gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat ttttatggag
     8161 aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg aatgctggac
     8221 tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg aatttccgtt
     8281 ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca gtggggcaag
     8341 aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta ccaatggatg
     8401 atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa aaaggctacg
     8461 aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt gcttatggag
     8521 cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg agtcttgatg
     8581 acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg aaataattaa
     8641 ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt ttttactttc
     8701 tactgtggta ttcatgataa acatgatata gattcaattc atattctttc ttagcgttcg
     8761 tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa gtcatcaatc
     8821 cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata tattcgcgaa
     8881 tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa atcccaaaga
     8941 aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact ttgaatccgt
     9001 cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa tcgattggga
     9061 tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt tcgatttgaa
     9121 aaccagaact cataatagga aaacttttga caaagatacg attgaatcct cgatagccgg
     9181 tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta acaaggttat
     9241 ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt gataggcgat
     9301 caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca tgtacagctt
     9361 ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg atatcaaaga
     9421 acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg attgctccac
     9481 cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca taaatatctg
     9541 cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct tcctcattgt
     9601 aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct ccttaactat
     9661 agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag tttttcccgc
     9721 taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc tctatctaaa
     9781 aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg gtgcgtattc
     9841 gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa atttctaata
     9901 cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca tcttctattc
     9961 gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata ggttcctttt
    10021 caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac ttgatatttt
    10081 caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt gaagctaccc
    10141 cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag cttagttcgc
    10201 tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg tgcttatttt
    10261 accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat aagctataat
    10321 aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat aagtctgaga
    10381 ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt caacgaatca
    10441 aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc gcttatatgg
    10501 aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc ggcatttttg
    10561 gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca catatggatg
    10621 aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc gtacctctag
    10681 gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc tctactggta
    10741 agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga acaagcggac
    10801 aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca agagaagagg
    10861 caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa acgatcaaaa
    10921 cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc tgtacaatgg
    10981 tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg attgcaggcg
    11041 cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat aaattcaagc
    11101 cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg aaaaatggca
    11161 cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt ttgatcatgc
    11221 ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc ccttatcaat
    11281 actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac gaaggaatcc
    11341 caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg atgttcagta
    11401 ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt ttggataaat
    11461 caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata aaatctggca
    11521 aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg ttagttcgaa
    11581 agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac aaagaatgat
    11641 tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga gtgtattttt
    11701 ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc ctgaaattga
    11761 aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca tagatgtagc
    11821 cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg gaaaagaatt
    11881 aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt tcctagaaag
    11941 tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat ataagaatat
    12001 tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag cggtagaagt
    12061 ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc aattttataa
    12121 ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg aaacagccaa
    12181 ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa atatagaagg
    12241 aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa aaaaatatga
    12301 gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct ttatgacagg
    12361 aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg tttctgacga
    12421 agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa tcaattaact
    12481 aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg aataaatggt
    12541 gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca gtgaatgcaa
    12601 ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga tttttgctta
    12661 taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata aaggaacaga
    12721 aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag gtcaagttgt
    12781 tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg gccaagtaag
    12841 cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag gattcgaaga
    12901 acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa catgcgtccc
    12961 acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg gtacagtatt
    13021 gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg ttgcaaaacc
    13081 aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag gggtagatag
    13141 ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag caaaaaaagg
    13201 aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat aaagaggttg
    13261 tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat tttgttttaa
    13321 tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc cttcatagta
    13381 agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa gttctacacc
    13441 agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca atccagcttt
    13501 ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta cagatgaagg
    13561 aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga ccattgtgat
    13621 acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga tattcactac
    13681 atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag tagattcaga
    13741 agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat taactaaagg
    13801 accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga tattgactgt
    13861 ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga ttcctgaagt
    13921 gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag gctcaccaac
    13981 cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg aattgatctt
    14041 caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta ccttcgtacc
    14101 gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat tcgtgactac
    14161 ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat aagcttccaa
    14221 attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc caaaaccgct
    14281 tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa ctttatctac
    14341 cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg tttcaccggc
    14401 agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca tcgctgcata
    14461 aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca gacctaactt
    14521 acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt ttctatccat
    14581 gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat ttgttgactt
    14641 tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc attgttttca
    14701 gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc ggataggatt
    14761 ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa attcttataa
    14821 aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt tttctgtgga
    14881 taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa atagccaaaa
    14941 taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag accaaaggag
    15001 agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat acctgatcca
    15061 aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat tgatcgaagt
    15121 tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag taaaagaaaa
    15181 agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg ccttagcaat
    15241 tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat taggtatcca
    15301 aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg aagccgtcat
    15361 gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc aattgatgcg
    15421 tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg ctccatacca
    15481 caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata gcaataggta
    15541 gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa gtaagttgca
    15601 aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata cgtaaaattt
    15661 tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt ttaaaaaatc
    15721 tattagagga tatggatgat attgaagaaa actatctaaa tggact
//
LOCUS       DARDHA010000057        15696 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000057 DARDHA010000000
VERSION     DARDHA010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.057"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(274..640)
                     /gene="ssrA"
                     /locus_tag="SDA10_001431"
     tmRNA           complement(274..640)
                     /gene="ssrA"
                     /locus_tag="SDA10_001431"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(811..1584)
                     /locus_tag="SDA10_001432"
     CDS             complement(811..1584)
                     /locus_tag="SDA10_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG4240348.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1728..2078
                     /locus_tag="SDA10_001433"
     CDS             1728..2078
                     /locus_tag="SDA10_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEG4240349.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2059..2775
                     /locus_tag="SDA10_001434"
     CDS             2059..2775
                     /locus_tag="SDA10_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4240350.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2775..4223
                     /locus_tag="SDA10_001435"
     CDS             2775..4223
                     /locus_tag="SDA10_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG4240351.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4293..4802)
                     /locus_tag="SDA10_001436"
     CDS             complement(4293..4802)
                     /locus_tag="SDA10_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240352.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4792..5517)
                     /locus_tag="SDA10_001437"
     CDS             complement(4792..5517)
                     /locus_tag="SDA10_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240353.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5740..7860)
                     /locus_tag="SDA10_001438"
     CDS             complement(5740..7860)
                     /locus_tag="SDA10_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG4240354.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8090..9268
                     /locus_tag="SDA10_001439"
     CDS             8090..9268
                     /locus_tag="SDA10_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEG4240355.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9284..10042
                     /gene="aroD"
                     /locus_tag="SDA10_001440"
     CDS             9284..10042
                     /gene="aroD"
                     /locus_tag="SDA10_001440"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEG4240356.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10065..10550
                     /gene="ybaK"
                     /locus_tag="SDA10_001441"
     CDS             10065..10550
                     /gene="ybaK"
                     /locus_tag="SDA10_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEG4240357.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10621..11874)
                     /locus_tag="SDA10_001442"
     CDS             complement(10621..11874)
                     /locus_tag="SDA10_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG4240358.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            11992..13341
                     /gene="gdhA"
                     /locus_tag="SDA10_001443"
     CDS             11992..13341
                     /gene="gdhA"
                     /locus_tag="SDA10_001443"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEG4240359.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13453..14799
                     /locus_tag="SDA10_001444"
     CDS             13453..14799
                     /locus_tag="SDA10_001444"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEG4240360.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15107..15493)
                     /locus_tag="SDA10_001445"
     CDS             complement(15107..15493)
                     /locus_tag="SDA10_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG4240361.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(15542..>15696)
                     /locus_tag="SDA10_001446"
     CDS             complement(15542..>15696)
                     /locus_tag="SDA10_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEG4240362.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
BASE COUNT         5123 a         2809 c         3161 g         4603 t
ORIGIN      
        1 gaaggaacat gcttaaacca taattgtgtt tttccttcca tcagcataaa agttcacctc
       61 aaaaagagtg tacgaacaaa agttcttttt gtaaacctta aaatagattt tcctgaatac
      121 gtattgggga tttctaaatg tttgaatgtt tttttgaatg caaaatcaat gcaaaaaatt
      181 ttgtatttct ctacttttct ctccttgacc aaaataaaaa aacgttgatt taacaacgtt
      241 tttcatacat gtttatctgt ctcaaccata catatatgga gacggcggga gtcgaacccg
      301 cgtccaaaca catcgccact taaaaatcta cgttcatagt caactcattt aaggattcac
      361 tttacagctt gccgagtgac aggcattctg tatcgctagt ctgatgttct ctttttcaac
      421 ttacagacgg aaagttgaaa cgtatcccac taaagttagg acccttactc gagcacatgg
      481 gcgatgccga gaggatctac gctaactgtt tttaggcagc taaagcgtaa gagttgtttt
      541 cgtttttagc agttatattt aactgtaacg tttttacgta gacgtaacct acgaaacgca
      601 attcaagctc gactgtgcct gtcgaatccg taacgtcccc gttagctaac aagggttacg
      661 tgtatttttc atacaaaatt agtatagcat atattgggat aaattcaata aaaaagcgaa
      721 tcatatccca ttatttatat aataactgcc cctttcttaa gaaaaaataa gcgattttct
      781 taagaaaaga ggtagcttca aaaaaagatc ttaaatcaga tcaaaatact tcaatgcgtg
      841 gacaattccg ccttctgtat ttttctttgt gataaaggta gaaagttctt ttaatccgtc
      901 ataggcgttg cccatagcga ttttgttgtc gcaagcttcc aacaaggcaa aatcattcgg
      961 accatcgcca aatccaaaag taggaacttt ttccagctga agttcttttt gcagacgttt
     1021 gacacctgcc ccttttgaga ttcctttctt cactgtatcg atcgagtaag gaccattccg
     1081 ataaaaagtt agctcaggaa aatgttcgtg atagtattcg tcacctgatt gagataagat
     1141 cagcaacata tttactgtgt tttctttaaa gcctaaagga tcaatttccg gaacatctga
     1201 atgaatgaag tcgtaagcat tgatcattgt ttggctatga ccggtacacc agattttctg
     1261 atcgttatag taagaaagtt catgaccgtt caactgtaca tgatcataca ttttttgaca
     1321 ttcttctgaa gaaaatgttt gagaatagat ttcttcgccg tctactcgaa tgaaagaacc
     1381 attcatggtg accgcagaag taatgcccgc atcttccatg atagagtgga tttctgcttc
     1441 tgtacgtcct gtagcgatga cggggagaac attgttttct tttaatgcgt tcatagcttt
     1501 tgccacttcg ggtgtgattt ttgatttcgc atctagtaat gttccatcaa gatcaaagaa
     1561 agtgattgca cgataatttg acacaaataa aactcctttt ttattggaat aagtttatct
     1621 taacaaagaa tgaacaaaag acaaaccttt cgtgaagaat tcctgaacat attggttcag
     1681 tcaacaattt agagtatact ataagaagag aatttaggag ggaaagaatg atttttttca
     1741 gtattggtat cttattctta ataattggca gtctttttcg gattctgccc tcaaaaggaa
     1801 atctgccttt ttatgggtat cattcgcctc ttgccgcaaa aacagacgct cattggcggc
     1861 tggctcaaaa gacaagcgga aactggtttt ttcttatggg cttactgatg gcattgatcg
     1921 gctattattt aaagacatct gggcatacga attattttct gattgaaatg cttctgctag
     1981 tatttccaat tatgcctatt tttattatga cagaaaagaa attacagaaa tatgacctgg
     2041 aaactggagg gaacgataat gaatatttta atgattgaag ataatcaatc tgtttctgaa
     2101 atgatgcaga tgtttttctt aaatgaaggt tgggaagcaa ctttcaagta tgatggaaaa
     2161 gaagggctcg atgccttttt agaaaaccca caacattggg atatgattac actagacctg
     2221 aatttgccca cactggacgg tatgctagta ggaagagaga tccgcaaagt ttcaagtact
     2281 gtgccgatta tcatgctgac agctagagat tctgaaagcg atcaggtgat cggcttagag
     2341 atgggggcag atgattatgt cacaaaacct ttcagtcctc tgacgcttat tgctcgaatg
     2401 aaagctttgc atcgccgtag tgagttggca gaagcaagag aagaagcagc agtttcagat
     2461 gatcattttg atatcgagac tagtcatttc aagatgaata cgaaaacacg agaagcgtat
     2521 ctggacaacc ggccaattga aggactgacg cctaaagagt tcgatctatt gtttacttta
     2581 gcaaaaaaac cacgtcaagt attctctcgg gagcagttgc ttgaattagt ctgggattat
     2641 cagtactttg gtgatgaacg gacagtggac gcccatatca agaaattacg acaaaaaatc
     2701 gaaaaggtcg gaccacaagt catccaaact gtttggggag tcggctacaa attcgatgat
     2761 tctggtgttg cctaatgcgt tatttgtatc agcaactctt agctttttgc ggaatggtcg
     2821 cgttgatctt attgatcgtt ggagtttctt ttacacaact gacaaaacaa acgattgaag
     2881 aaaacaatta ccagcaactg tttggatatg ctgaatcagt tgaaaagaca acgcaaacct
     2941 atgccgatac gctcccgcag tttagtcaag atcaggcgtt tcaaaatgca ttgctgctga
     3001 cagagcaagt actgacagaa caaaatgtga actttatatt tatcgataaa aacgaacgtg
     3061 tcatttatcc gactaataca gctttactga attttacctt aacagctgat caatggaaaa
     3121 atctgaggaa tggacgtcaa gaaaaattca catcgaataa aaatatttta ggacaagcac
     3181 aggcgacctc ttacgcactt gtgccattta atttgaatca tgaattctat ggagcacttg
     3241 tagttagtca acctgcgcga aatatcgata acagcgttcg tccaattaca ctgaacctgt
     3301 tcaaaggatt tattttttcc agtatcgttg cagttattgc cagctatgct tttgcagcat
     3361 tccaagttaa gcgtatcaat cggttaagga atgcgacgaa agaagtgacg aatggtaatt
     3421 ttgatgtaca gctccccgta catgacaaag atgaatttga tgagttagca gatgatttca
     3481 ataaaatgac gaattcattg aaagaatctc aagcagaaat tgaagaacaa gaaaatcgtc
     3541 gaagacaatt tatggcagat gcttctcacg agatgagaac acctcttacg acaatcaacg
     3601 gacttctgga agggttagaa tacaatgcaa tccctgaaaa tcagcgagaa aacgcgatta
     3661 aattgatgaa aaatgaaaca gagcgtctga tccgtttggt gaatgaaaac ttggattatg
     3721 aaaaaatccg tacgaatcaa atcagtatgg tcattaaaaa attcaatgga acagaaacat
     3781 tgaaaaatat tgttgcacag ttagaagcta aagcagaagc agcaggagat acattgacat
     3841 tgaaagccgt tgatgatatt gatgtttacg ctgattatga ccgatttgtc caaatcatgg
     3901 tcaatattat tcaaaacgcg atacagttta ctgaaaacgg ccaaatcacg attactttag
     3961 aaaaaggcta tcttgaaacg atcatcacga ttgaagatac gggaattgga atgtctgaac
     4021 aacaaatgaa aagtatctgg gatcgttatt ataaagtaga tccttcaaga aaaaatacaa
     4081 aatatggtga atcaggatta ggtttaccaa tcgtgcagca acttgtacgc ttgcataaag
     4141 gaaaattaga agtagaaagc gaactaggaa aaggaacaaa attcgctgtt cgtttgcctg
     4201 acgtagaaat caaaaaagag taaaaaacaa gcggtataaa agatcatgaa tcgatcttct
     4261 ataccgcttg tttttactat aaaatgattg atttagcccg cgggctctaa acgatcaata
     4321 taaggacttt gagcaatagt agcatcatga gtaaagagta agataccttg attttcttca
     4381 tgagctaaat aagaaagcaa gtggagtagc tcttggatct ccaatacact aagttcatca
     4441 aatgtatctt caataacaat attgtttcgc ttggaaagca aggcatgaag taattgcagc
     4501 tttacttttt caaaagacgt caactcagct gccgcacaat taagcaagga aggagagata
     4561 tcaagttttt cttgataggc caaacgatct tgtttgattt ctttttccgg tacatccaac
     4621 aggacatggt ctttaagatt cagataagga aacatcggcc aatttttttg gatgatcgca
     4681 aaattatcca gttttgcaac aagaacctct tttttcaagt gctccaatat tttatgctgt
     4741 ttttcgagag gacacaaaat aacggtcaag tgttcagcat gaatcaaata ttcaggtttc
     4801 attacgtcca ggcctcctac tttgcaaatg atggataaaa aaagtgaaag ggaatagact
     4861 aagaatgaga cagcatccac tgagcccaat aagagtttgg gcaaatccct taagtatatc
     4921 tgtgaaaaaa agatcatttt ggaaatcgat ggtaaaaaaa gtctgttgat ccatcggaat
     4981 tgtaagtccg cttaccgaag tcaagatatc acggatatct ggcagttcag tagtctttaa
     5041 gacaagaaaa ttgatccttt gaagaagcgc ttcataaact gactggaagc ttacgatcca
     5101 caataaaacc aagatacaac aaagaaagag aggaaagaac agctgccaga aaataaaaag
     5161 gaataatttg gttttagaaa gaccagtcag gcgccaattg atcaattcct gcttgttttg
     5221 cctgactgaa tagtaaaaga aaaagataaa aaggaaggca aatagaaaaa ataaactgaa
     5281 tatgcctttt atataaaatc ggctagctaa ttctaaagcc ggaagaagct ctgtagatgc
     5341 agaagcgcca ataaatgaaa gccgatcttg gacttgattg gctaaagccg attgcaaatg
     5401 gtaaagagtc aggcatgcta tcaataaaaa agcagaaaag cttgaaaata aacaatagag
     5461 tattgtctgc tttttgtaat aaattaatcg agcagtagca tagcggatta ttttcatact
     5521 tttcacctct ataaaaagta tagaaaatga atatgaataa agtatgaaat tcctttagtg
     5581 aaatcacgaa ttaaaaccag aaaatatttt gtaaaaagca agaggttgtg aagaagctgc
     5641 cctattctat taccgtagat gcaagctttt gaacactgtt tattcgaaaa gaggagcagc
     5701 gacaataatt ttgtcgctgc tccttttaaa tttattttgt cattcggaag aacttgtggt
     5761 cgttccgtga agttcttggt acgtttgcgt tttataaaga tcaacagttg attgtccgtt
     5821 gtgttcacta aagacactag ttgacttgtc accaagagat ttttcagctt cttttaattt
     5881 ctctaagcca ttcttgtagt cataatcact ggtatcaatt gggtctaatc cactattcgt
     5941 atagaaccga agaagatcac cattattgat ttggtcagaa acagctaact gtttatcaac
     6001 tttttctttc agtgaatcaa tttcattctg tacttcctca gtaggttcag taatagcttc
     6061 accagtttcg ttactataga tcgtaccacc gtagtaagta tattttggtg taacaaaatc
     6121 accatcacgg aaagaaacaa gctgttcatg atctttcgag aatagatctt gtcctaactg
     6181 aatataattt tttgtatcta cacctagtaa gtgaagtaat gtaggcaagg catcaacttg
     6241 tccgccgtaa gtgtgattga ttccgccctg tgtttgtcca gggatatgga tcatataagg
     6301 gacacgctgc atttgtgcat tgtcgtagtt tgtccaagta gaacttgttt ttccaactaa
     6361 ttcagctaga tttttatttc ttgaattcga gacaccgtaa tggtcaccgt aaaggacgat
     6421 gactgaatta tcatacaagc cgctttcttt caaataattg aagaattctt gtacggcggt
     6481 atctaaataa ttggctgtcg caaagtaacc gttgatcgtt tcatcatttg tattggctaa
     6541 cgggaatcct gcttcgtcat tggtgaattt tgaataagga taatggttag atacagcaat
     6601 gaattttgaa taaaatggtt gttgcagacg ttccaagtat tgtacggatt gttggaaaaa
     6661 tggtttatca tgcaacccgt attggaacga attgtcatca ttaacatcat aataagaaga
     6721 atcaaagaaa taatcatatc ctaaattctt gtatgtctcg tttcgattcc agaatgtacc
     6781 agcgttccca tggaataccg cactagtgta accttgtgtt tgacttaaaa tatcaggtgc
     6841 agcttggaaa gtattttttc cgcctaattg tgtgaacaag gagccttgat tcaagccaaa
     6901 caatgaattt tccatcagcg tttccgcatc gcttgttttt cctgctttta cttgatggaa
     6961 aaagttgtca aaactgaacg tgctgttgct gtggtaaaga ctattgatga aaggtgtaac
     7021 ttcatgctgg agaccattct catctgtcaa tttataatcg atcaggaatt gctggaagct
     7081 ttctaaatgg atataaatca cattttttcc tttagcgagc ccgaacaaat cgtcatttgg
     7141 tgctgcataa tgttctttta cataattttc tacttctttc atatcattgg gacttgcttc
     7201 tgcccgcact tggtttgtct ggtaagtttg aacaccgtca taaaccatga aagcattcaa
     7261 gccaagatat ttgaccaaat aatcacgaga gaatgttcga gtcagcaatt ctggacgatc
     7321 agcttccgcc ataaaaagat ttcctgagaa aatcataact gctaatgtag agatcgcaaa
     7381 agccattctt gctcggatcg ggcgagggtc caacttgatt ttttttgcaa tcagcagaac
     7441 agtcaagata ataaaatcga tccaatagat cacatcatat gggcgaaaca gtcgaatggc
     7501 actttctccc agaccactcg ctactttccc agcacctaaa atcgtgttga tcgtaatgaa
     7561 gtccgtgaat tctcgataat aagtgacatt cgaaaaaaca agcaatgtca aaagaaaata
     7621 aatgaccatc atcgtgatat aagcaactct agggcgtctg atataaagag caattccaag
     7681 taataacaat gtagttgcaa tagggttgat aaacaagata aagtattgaa tgccactttc
     7741 gattccgaga tggaaatcaa ctaaataggc aaaaatcgtt ttcaaccaaa gtaaaacgac
     7801 tagtaaagaa aaaaatccga gcctcttatt caaaaaggca ggtgttttta tacatttcaa
     7861 gataatcttc ctttctgcac ttcttaatca cagaagtgtc atgaaaccat gttacacagc
     7921 tcacttattc tcatttattt tactagtatg acaatatagt gtcaattgaa accagaagtt
     7981 tatgctgttt cattaaggtt ttctttaagc ttcatgaaaa aatgagcagt aagacaagtc
     8041 ctatgttttt tgttatactt gggaagagaa agctaaagca ggtgaaggaa tgaaagtaaa
     8101 agtaaaaaaa agagcaagca atcgattgaa aaaaggatat ccactgatcc aaaaagaaga
     8161 ccttgctcat ccccaaaaag aaaccacgca atgggtggaa ttagttgatt cgcaaaacca
     8221 atttatcgca aaaggatatc taggaaagca gaataaggga atcggttggg tattgagcca
     8281 gcagaacatc ccatttgatc aatctttttt tgaagaattt tttgtgaagt caaaagaaaa
     8341 aagaactttc tttttttcag atgattctac taccgctttt cgtatattca atggagaagg
     8401 agacggaatc ggcggtctga ttgttgatta ttatgcaggt tatgctgttt tttcttggta
     8461 taacgaaacg ttatatgcac atcgtgaatt attgattcaa gcttttcgtg aaatctaccc
     8521 ggaaatcgta ggaggctatg agaaaattcg atttgaatca gtaaatctgc ctgagagcca
     8581 gttcatttac ggaaaagagg caccggaacc cttgatcgta aaagaaaacg gagtaaaatt
     8641 tgccgtttat ttaaatgaag gattaatgac tgggatcttt ttagaccaaa aagaagtacg
     8701 gggatcatta gcagaaggtt tcgcttctgg gaaaacagtt ttgaacatgt ttagttatac
     8761 cggcgccttt tctgttgctg ccgcaatggg aggggcaatc gaaacaacaa gtgtcgatct
     8821 agcaaaacgc agtttaaaaa aaacacagga acagtttgaa gtgaatggtt tggatgtcga
     8881 tcagcaaaaa atcattgtta tggacgtatt cgagtatttc aaatacgcca aaagaaaaca
     8941 atttttatat gacatgattg tattggatcc accaagtttt gccagaaata agaaaaaagt
     9001 ctttcgcgta gcaaaagatt atggggaact ggtcaaggat agtctggata ttttagcaga
     9061 agacgggata cttattgcgt ctacaaatgc tgcgaatgtc tcgatagaac aattccaaac
     9121 aatgattgaa gaggaattcc atgccaaaaa tgtttctttt gaacaacttg ctttatatcg
     9181 tctgccaaaa gatttccaga cgacggatac ctttttagaa ggcaattatc tgaaagtgtt
     9241 tgtttatcaa attaaaaata accattagaa ggagcgatgc agaatggttt gggtaaaggg
     9301 attgaatata ggaagcggta gaccaaagat ttgtgcgcct cttatcgggg ccgtccgaga
     9361 ggaagtatta cgtgaagcag ttcttgcacg tgaagcaggg gccgatttag tcgaatggcg
     9421 agtcgatcat tatcgtgaag ttttttcttt tgatgaagta gcagaaacat tggttcttat
     9481 ccatgagatg cttgatggac tgccgttatt atttacgttt cgtaccttag ctgaaggcgg
     9541 agaacaagac atatcgatcc gtcagtatcg cgacctttat gaacatgtga tacacacagg
     9601 attagtagat atggtcgata tcgagttgtt caaagtagaa agtctaggaa aaagactgat
     9661 agaggaaatc aagtctttaa acattccttt aattatcagt agtcatgatt ttaaagaaac
     9721 accagcagac cctgtcttgc tttaccgatt gaatgttatg gaacattttg gagccgatat
     9781 cggaaaactg gcagtaactc ccaataatga acgagatgtt ttgcggttga tggagttgac
     9841 tagacgggca aatgcttttg tctccatgcc tattattacg atgtccatgg gaaatcttgg
     9901 taaaatttct cgtctggctg gcggaacgac gggttccgtt atgacatttg gttccttaag
     9961 cagcgattct acttctgctc caggacaatt accggtcaaa gcattgaagc aaattattca
    10021 attattaaat gaaccgttgt agaacaagtt tgaggtgata ttttttggca aagaaaaaaa
    10081 tagtaaaaac gaatgcaatc cgtatggtcg aacaaaaaaa gattccctat acagagcacg
    10141 aatatgagtg ggacgaaagc cacctaagtg cgtcatcggt cgctgaacag cttccagaaa
    10201 gccaatctcg tatatttaaa acactggttg ctgtgggcaa tgtgacgggg cctcttgttg
    10261 ctgtgatacc gggagaagcc gaattgaatc taaaaaaatt agcaaaagtt tcagggaata
    10321 aaaaagtaga aatgctccat ttaaaagatt tggaagcaac gaccggatac atacgtggtg
    10381 gctgctcgcc tataggtatg aaaaaactat ttcctactta tttagatcaa attgcagaaa
    10441 gctatgaaca aatcattgtc tcggctggca gaagagggct acagatggag ttagcacctc
    10501 aagatatctg tgcattaacg agcggacaat ttgcagatat aaaacaataa atcatacgta
    10561 taaaagaaag aaggaggccg ggatgttgtt tgtccagtct ccttctttct tttttataca
    10621 ttatgctctt tgcccggcat tcattcctgc tacatgccct gtagcgaaag ccgctgtgat
    10681 attaaagccg ccagtataac cattgacatc cagtaattct cccgcaaaat acagaccatc
    10741 aatgatttta ctctccaatg ttttaggata aatttccttt agagaaaccc ctccgccagt
    10801 tacaaatgat ttctcaatag gaaaagtttt gttgattgat agcttaaact ctttgcatag
    10861 ttgaacaaaa tcttgaattt gtttttgaga ggcttgttgt cctgtcagat gatccatttc
    10921 aagccgctca agaaagaaaa ctaacaatcg ctcaggcaat ataccatgcc aagcattgac
    10981 caagttcttt ttggttgctt tacttttttc tgtcaatata tgaataagct cttgtttggt
    11041 ttgtgtaggg aaacaatcca gagaaagtgt cactggttct cccgttttct ctaattcttt
    11101 attgacaaaa cttgaacaac gcagtgcagc cggaccagaa ataccgaaat gggtgaaaag
    11161 taaatccatc gtatgttcag tcaatacacg acctttttta tttaaaacgc gtaaagtgat
    11221 gtcttgtaaa gaaagccctt gaagtgtttt ctcctgaata tacggttcat ccgatatcaa
    11281 gggggactct gtcgcataaa gaggcgtcac cgtgtgaccc actcgcttca cgatcttgta
    11341 cccatctcca gtagaaccag tagatggata agtccgtcca ccagttgtta agataatggc
    11401 aggagcatgg aactcttcga attccgtgcg tataccgtaa attttatgtt ctttgtggat
    11461 caatttctct actcgagtag agaatagaag cgtcacatct agttctttca gacgattgag
    11521 caatgcttcg ataatcgttt ttgatttatt tgttacagga aacatccgtc catgatcctc
    11581 ttcttttaaa tgtacgcctt gtgattcaaa gaagttcatg atatcgaaat tattccattg
    11641 cgcaaacgta ctatataaaa actttccatt tccaggaata tgtgcaatca aatcatcaac
    11701 aggacgattg ttcgtaacat tgcaccgacc accacctgtc atcagtagtt tttttcctgc
    11761 ttttttgttt ttttcgatca agagtacgcg tgcgccttgt tcagctgcac tgatcgctgc
    11821 catcatcccg cttgttccag cgccgactac gatcacatca tacagttttt ccattccctc
    11881 acctcaagga aagtgtagca tatctgaaga aagaaaacag aaaaaaaatg ttttgagcca
    11941 aaagagaaaa tgagctaaaa tgagggaaga taagcaaagg agcgtggaac gatgacaaac
    12001 gcaacagaat atgttcaagc aattcaagaa aagctacatc aaaaggatca agggcaagaa
    12061 gaatttttac aagcaatcga tgaattcatg cccacggtta tgtctttttt ggacactcat
    12121 cccgaatata tcgaaaagaa tatcttagaa cttttaacgg aaccagaaag agttattcaa
    12181 tttcgagtgc cttggcaaga tgatgaagga aactggcgag taaatagagg gtaccgtatc
    12241 caatacaact cagctatcgg cccttataaa ggtggattgc gcttccatcc tagtgtcaat
    12301 ctaagtatct tgaaattttt ggcttttgaa cagattttca agaatagttt aaccggtctg
    12361 ccaatcggtg gaggaaaagg cggaagtgac ttcgatccta aaggaaaatc tgataatgag
    12421 atcatgcgtt tttgtcaaag tttcatgctg gaattgtcaa aacatatcgg cccatcgatt
    12481 gatgttcctg cgggagatat cggtgtagga gctagagaaa tcggctattt gttcggcgaa
    12541 tataaacgat tgaaaaaata tgatactggt gtgttgacag gtaaaccttt ggatttttgg
    12601 ggaagtaaga tacgtacgga agctactggt tatggattag tgtattatgt caaacatcta
    12661 ttgaacgaag aaaaagattc atttaatcaa aaaacagttt ttgtttccgg aagcgggaat
    12721 gtagccattt atgcaatcga aaaagtgcaa gagctaggcg ggaaagttgt tacatgttca
    12781 gattctagcg ggtacatcta tgatccagaa ggaatagatg tgaaactatt gaaagaagtc
    12841 aaagaaatca atagacaacg tttgacggaa tacgcaaacc aacgtccttc tgctatttat
    12901 catgctggag aatctgtttg gacattgaaa gaaaaagcgg atatcgcttt gccatgtgct
    12961 actcaaaatg aaatagacga aagcttggcc gaaatcttag tagaaaatgg gatcaaaatc
    13021 gtagcagaag gcgctaatat gccatgtaca gtaaaagctg tttctgttct tcatgaagca
    13081 ggcgtgtggt attgtccagg aaaagctgct aatgctggag gagtagcagt atctgcttta
    13141 gaaatgagtc agaacgtcca gcgattgact tgggaaagag agcaagtgga tgagcagctg
    13201 gatcaaatca tggaaacgat ttaccatacg tgtcgccaaa cagcagccga gtacggcgac
    13261 gaaaaaaatc tgcttcttgg agcaaatgtt gctggatttg aaaaagttgc caaagcaatg
    13321 gcagcagaag gtttagtttg aaaaatgaaa ctttcttgga aatattgtca gcattttgtt
    13381 tatttgcttg aaaaataata aaaaaaaacg taaagtatta cctgtagagt aaacctataa
    13441 ggaggagaac aaatggcaca cattcatttt gactattcaa aagtagcacc gtttgtcaac
    13501 gaacaagagt tagggtacat gcaaagccaa gtaacagcag cccacaacga attacgggaa
    13561 ggtactggcg caggaagcga cttccgcggc tggatcgatt taccaacgaa ctatgacaaa
    13621 gaagaatttg cacgtatcaa agaagcagct aaaaaaatcc aatctgattc tgaagtatta
    13681 gttgttatcg gtatcggtgg ttcttattta ggcgctcgtg cagcgatcga cttcttgaat
    13741 cacacattct tcaatttatt agatagcaac acaagaaaag caccacaaat cttctttgcc
    13801 ggaaattcaa tcagctcaac atatattgct gacttgatcg aagtaatcgg tgatcgcgac
    13861 ttttcagtaa acgttatttc aaaatctgga acaactactg aaccagcgat tgcgttccgt
    13921 gtattcaaag agcttcttgt taagaaatat ggacaagaag aagcaaacaa acgtatctac
    13981 gctacaacag ataaagcaaa aggtgcagtt aaagtagaag cagatgcaga aggttgggaa
    14041 actttcgtga ttcctgatga catcggcgga cgtttctctg tattaactgc tgttggtttg
    14101 ttaccaatcg ctgttagtgg tgcagatatc gatgcattga tgcaaggtgc cgctgatgca
    14161 agcaaagcat actcaagcga caaactagaa gaaaacgaag cttaccaata cgcagcaatg
    14221 cgcaacattc tttaccgtaa aggcaaagta actgagttgt tgatcaacta tgaaccagga
    14281 atgcaatact tctcagaatg gtggaaacaa ttgtatggtg aatctgaagg aaaagaccaa
    14341 aaaggcatct atccttcaag cgcaaacttc tcaactgatt tgcactcttt aggacaattc
    14401 atccaagaag gacgtcgcaa tatttttgaa acagttgtga aggttgaaaa acctcgtaaa
    14461 tcaatcacaa ttcctgaaca agcagaagat ttagacggtc taggctactt gcaaggaaaa
    14521 gaagtggact tcgtaaatac aaaagcattt gaaggaacat tgcttgctca tacagacggt
    14581 gacgtgccta acttgctagt caaaattcca gaaatggatg cttatacatt gggttataca
    14641 atgtacttct ttgaaattgc tgtaggtatt tcaggttact tgaatggaat caacccattc
    14701 gaccaaccag gtgtagaagc ttacaagaaa aacatgttcg cacttctagg caaaccaggc
    14761 tttgaagatc ttgctaaaga attgaacgaa cgcctgtaag ataagggttt ttcgctagat
    14821 ttaaataatc agtgatagaa acatgttcgc tttaaaatga aaactggtac ccatttaggt
    14881 actcaaacat actgcatcgt ttattcagaa taaggggctg tgacaagacg tttgtcacag
    14941 tcccttgttt gtatagaaat cgattgattg ccttgctctt tagtttgttt taggacttta
    15001 agaacagtga ggctgacttc gaaggttaat tctgtttttt tgttttctag aatcagtaag
    15061 caaaaaagca ctcacttcga gtgctttttt gcgatatatt cttagcttaa gataggccta
    15121 atttttcttt tacagatgag ggtaccttat cgctggtaat tacttttttc tctatgatcc
    15181 gcttttcgct tgctttcaac tgcaagatcg ttccttcggg gtaagcagaa ccattggcag
    15241 agtcaccaga agtaatgaca tcgaaatcta actgtctttt ttcaccgttt tcagcataag
    15301 catctacgtt gtatttaaaa cctcttccaa ttacttcccc attatctgct ttgatatcag
    15361 tttctgcagg cattggagct ttgactaccg cataataatc tttacctaca taagtagact
    15421 gatagtagtc ccaacctttc catcctataa aagcaatcaa tatgataacg atcaacccta
    15481 aaattttttt catgaaagat ctcctttttt atttttatta tacccgtggt gctagttaat
    15541 tttaatatcc taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata
    15601 gaacactgta agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg
    15661 agttgaaatt ttgacaacct aatttttcta aggaag
//
LOCUS       DARDHA010000058        14815 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000058 DARDHA010000000
VERSION     DARDHA010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.058"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(296..1024)
                     /locus_tag="SDA10_001447"
     CDS             complement(296..1024)
                     /locus_tag="SDA10_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEG4240363.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="SDA10_001448"
     CDS             complement(1002..2576)
                     /locus_tag="SDA10_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG4240364.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="SDA10_001449"
     CDS             2805..3743
                     /locus_tag="SDA10_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4240365.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="SDA10_001450"
     CDS             3755..4666
                     /locus_tag="SDA10_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4240366.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="SDA10_001451"
     CDS             4690..6162
                     /locus_tag="SDA10_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG4240367.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="SDA10_001452"
     CDS             6213..6824
                     /locus_tag="SDA10_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG4240368.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="SDA10_001453"
     CDS             6854..8767
                     /locus_tag="SDA10_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEG4240369.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="SDA10_001454"
     CDS             8895..11876
                     /locus_tag="SDA10_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HEG4240370.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="SDA10_001455"
     CDS             11873..13534
                     /locus_tag="SDA10_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HEG4240371.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="SDA10_001456"
     CDS             13574..14506
                     /locus_tag="SDA10_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEG4240372.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DARDHA010000059        14394 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000059 DARDHA010000000
VERSION     DARDHA010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.059"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..777
                     /locus_tag="SDA10_001457"
     CDS             <1..777
                     /locus_tag="SDA10_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG4240373.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDE
                     FFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            926..1264
                     /locus_tag="SDA10_001458"
     CDS             926..1264
                     /locus_tag="SDA10_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240374.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(1469..2044)
                     /locus_tag="SDA10_001459"
     CDS             complement(1469..2044)
                     /locus_tag="SDA10_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEG4240375.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2776..3357)
                     /locus_tag="SDA10_001460"
     CDS             complement(2776..3357)
                     /locus_tag="SDA10_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4240376.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(3540..4166)
                     /locus_tag="SDA10_001461"
     CDS             complement(3540..4166)
                     /locus_tag="SDA10_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG4240377.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(4188..5519)
                     /locus_tag="SDA10_001462"
     CDS             complement(4188..5519)
                     /locus_tag="SDA10_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEG4240378.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(5674..6075)
                     /locus_tag="SDA10_001463"
     CDS             complement(5674..6075)
                     /locus_tag="SDA10_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG4240379.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(6161..6367)
                     /locus_tag="SDA10_001464"
     CDS             complement(6161..6367)
                     /locus_tag="SDA10_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240380.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(6462..7964)
                     /locus_tag="SDA10_001465"
     CDS             complement(6462..7964)
                     /locus_tag="SDA10_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEG4240381.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7982..8437)
                     /gene="argR"
                     /locus_tag="SDA10_001466"
     CDS             complement(7982..8437)
                     /gene="argR"
                     /locus_tag="SDA10_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEG4240382.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(8535..9152)
                     /locus_tag="SDA10_001467"
     CDS             complement(8535..9152)
                     /locus_tag="SDA10_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEG4240383.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            9373..10746
                     /locus_tag="SDA10_001468"
     CDS             9373..10746
                     /locus_tag="SDA10_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4240384.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10821..11933
                     /locus_tag="SDA10_001469"
     CDS             10821..11933
                     /locus_tag="SDA10_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEG4240385.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(12005..13642)
                     /locus_tag="SDA10_001470"
     CDS             complement(12005..13642)
                     /locus_tag="SDA10_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEG4240386.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13810..>14394)
                     /locus_tag="SDA10_001471"
     CDS             complement(13810..>14394)
                     /locus_tag="SDA10_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG4240387.1"
                     /translation="LGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKISQKFAGDN
                     SMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTETATQEHTYV
                     APVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQLDASYLNGD
                     YSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3929 a         3000 c         2602 g         4863 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtaga tgaatttttt
      661 attcaatctg tcgcatctaa acgaaataat attcctagaa aatcactgga ttatcgaaca
      721 cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt aatttgacaa
      781 ttaagaaact aaaaaaataa tccaagaaac attgaaccta tggatccaaa agctacccaa
      841 atactatagc taaccccccg ctaccttcaa ttttgcagat aaaacgcttt gggagaaaat
      901 ttcaaagaca acaggccatc ctatcatgcc cgattataaa caaaaagctg gctattcctt
      961 ttgctctgac aaatttcaat tgggaaagtt cacgaaacta gcaggtgaag aagtttcaac
     1021 tgttcggatc aaagaatgcc cgcttcaaat agaaacattg gttcaacgaa tttcaactag
     1081 acaagacttt gctattgtag aacgtgagat cgtagctatt tttgtagaag aagggatttt
     1141 acatgatcca actcatatca atgtggataa atggcaacct cttatctata aattcagaga
     1201 atatacaact gccaaccagt cgttaacttt gaattttaga ttccaagaat tcaaagagtc
     1261 ataatgataa acatcgttag gtataaataa aaaagactcg cagcacgagt aaaaaacaaa
     1321 tagaagctat acattagaaa ggtcgactgt gaccctatag atgcagtata tgcagcttat
     1381 gagcatttct tctgttctct ttgtttttgg gatctgtgct gaagtctttt tcgttttttg
     1441 ccgctctttc tttgaaaaat cgattatgtt ataccttctc cattactaat tcaggcatct
     1501 gctgagtaat gatttttcgc gcaaaagaaa tatctgttac ccaaggttcg atttgttctt
     1561 cagtcaagac aagcggcatt cggtcatgga ttggtgcgac tgtgcggtta ggttcagtag
     1621 tcattatgat tgattctgcc attttttcat cctgatggct acgataaaaa ccgccaatat
     1681 acactatttt ttccttattt gaaaacataa atttgtcttt ttcttcattc cactcataaa
     1741 agccactcat tgggaaaaca caacgatttt gcaaaaatgg ttgatgaaag gtcttttttt
     1801 cttctactgt ctctgcacgg gcattaatca tcaattgtcc ttttttaaat ccggtaaatc
     1861 cccacttcat gatttccgga gtgaggatgt tttcttcctc ttttcttaac gtcacaacaa
     1921 gatttgacgg aaaaacttct ccagctgcta attctgctgc ctgcttcggt tgtttcgtct
     1981 gggcttctcg ataataattc tgaaggagct ctgactctag atcaaaaaaa taacgaccac
     2041 acatcctaat ccctcccaat ctttttttct atcatacact gcaacaagaa gttcgcacca
     2101 acaaaacgct ttcaattttt gtagaactaa aaaagttgtg acaagtgaca aactctccag
     2161 tgaatggtat tctcctgcca actcctcggt cacaagtcac aatattcaac aaacatgaga
     2221 agctgctttt tgaaattgtt ccttgtgact cggagtgaaa cggcaggctt acgacctctg
     2281 ggtaaacggt gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtag
     2341 aaagccattt tcacaaaatt tttcttaata ctcgaaacta agcgactttt tcacaacctc
     2401 ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca aacgaaaaat
     2461 atgagcagct atttttattt ggctgttctt attacttgat gcagggcagc tttttcacaa
     2521 cctctccact aaacggtgtt caatcagcta accttcggta ttagctcaaa aaacggcaaa
     2581 atatgaggaa ctattttgcc gtttgtttct ctaatactca ggtcaaagcg ctgatttcac
     2641 aacctctaat acacggtgtt ccagaagtag cttcttcgaa aaataagccg aaatttcaca
     2701 aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga ccacttctgt
     2761 cacaacctct aattattaaa gaaccttttg cgaagttaac aatctttctt tgatcaattg
     2821 aaaattctgc ctaagtagtg atagatcctg tgtgactttc aacaataaga tacttccctg
     2881 gatttcagca atcaacgctt tggcttgacg gtctgcttct tcatcggaca gtttttcttc
     2941 agaaagtgcc aggatatttt gtttcgtata cttgacccat tgattgacta attctagtag
     3001 caacagacgg tattcttcat ccactgtact caactcaata atcaaattac ctacaggaca
     3061 gccatgataa gtgatctgtg tctcatgatc agcgaaaatc cagtccaaca tttcttctaa
     3121 tgcttcttct ggctgttttc cagcatttaa gatgtcttcg aacaattctg tctgccatac
     3181 gtccatatgc gcatggatca ccgctagaca aacttctttt tttgaagaaa agtaataata
     3241 aaattgacct tttcctgtat cagaagctgc tagaatatct cggattgacg tttcatgata
     3301 gcctttttta taaataagat ctttggtcgc ttccaagatc ttttcctttg ttttcaatga
     3361 tattctcctt tttcatttct catagtccta tattatacag taaattcatc attttttctt
     3421 cttaatcgtt taaaaaagac tgtgccaaat tatctgacac agccctcact ataaaactgt
     3481 tctcatatag ctagatgttc attgattcaa gaactgcgag gacaaaagca attcctatct
     3541 tatgatagtt gctctaatac ttcctctgtt gtcatcactt gatctgcaat aaagccaaag
     3601 ttcgtcaacg ctgcatccat tgcttcttgg ccaggagcac caattgcatc tgtcgctacc
     3661 acaacattga atccactctc taccaattca cgcatatggg aatcgctaca caagtttccg
     3721 ttcattccac caagaattac tgtgtcgatt ccattttttc tcaattggag cgccaaatca
     3781 ttgctttctg gtccaaagat tttatgagga gagctgatga tcgtattttc atccaaataa
     3841 ggtttcaatt cttcgataaa atcggctcct gaaccttctt ccactgattc atattgactt
     3901 tttctagcaa acatctgctg ctcaagcatc atatgttctc ctgcaccgcc aaattgccat
     3961 ttatggtcat gtgggtaaaa ataatgagga gaaataaaga gtttgtatcc tttttctttt
     4021 gcttgtttta ccatttcaat catattttcg atcgtattgt atttttctaa gatatctttt
     4081 gtcaaaccaa agccaccgcc tgctggtttc aagaactcga cttgcgggtc agtgagaact
     4141 actgctgtat ttttattatt gaacatggtg gattctccat tcgttattta ttttaataca
     4201 tcatttagag cttcactcat tgtcggatga gaatagattt gatctctcaa taacgtataa
     4261 ggaagttttc cattcatagc tagcgaaatc aagttgatga cttcatgaga atcttccgca
     4321 tagatacttg cccctagaat caagtcagtt tcagggtcga ctaaaacttt gaatacacct
     4381 ttaggatctt ctaatacttg tgctttaggg acaccgcttg tcatatactt gaataatttg
     4441 taattgacgc cttgtgcttt ggcttgtttt tctgttaaac ctacattgga taaagtcgga
     4501 gtgataaata cactgtaagg cacagctttc cggttattcg ttgttcgttt attctcgccc
     4561 ttcaactgat ccataatgat tcgataatca tctagagaga tataagtgaa ctgcaatccg
     4621 cctttgacgt ctccaatcgc ccaaatgttg tctgcatctg tacgaaggta ttcatccaca
     4681 gcgattgctc cgcgatctgt tagtttaaca tctgtatttt ctaatccaag tgatgctgta
     4741 cttggttttc ttccagttgc agccaaaact tttgacgctt ggattgttac ttcttggcca
     4801 tcttctgtaa aggtgacggc agctgttgat tcttggtctg ctacttgatc tacagataca
     4861 cctagatgga attcgattcc cgcatttgtc atgtcttcta aaatcatttg cgcgatatct
     4921 tcatcttcac gtggaagaaa ttcagaatgg gcatcaagaa cgacaacttt agatccgtat
     4981 tcattgaaca ttgatgcaaa ttccaagcca atatatccag ccccgatgat cactaatttt
     5041 tccggtaatt gttcctgatc catcgcttgc gtagaatcaa tcaagtagcg acttgtattc
     5101 aatcctttga ttggtaaaat gattggttca gaaccagtat tgatgaaaat ccgttcgcct
     5161 tcaacaatca ctttttcacc attattcgtc acttcgattt gatggttaga aagaaaacgt
     5221 gctgtaccgt ccatcactgt cccagttgcc tcatcgctga tcatatgata atttttggcg
     5281 cgaagcatgc ccgtcaattt ttctttccga gcaacagcct ctgtaaagtc gattcctctt
     5341 tctccattga aaatcaatga tttagaagga atacagccga tgttgataca tgttccgcca
     5401 tacatacgtg ttgacttttc aacgactaaa accgattcac cttttgaagc taacgccttc
     5461 gctaaagtct taccgccctt tccaaaaccg atcacaatat tttgatattt ttccatcatt
     5521 tttccctcgc ttctttttta cctaccgatt ggtacaaaaa tatgataacc gataattgtc
     5581 tattctccaa atcatctgct ttatttgtca aaaaaaacca gacaaaaagc ggtcctttca
     5641 cgtttttagt ctggttcgat ttcctttatt cttctaaaca atcgttactt ctacatcgat
     5701 atttcctcta gttgctttgg agtaagggca gaattcatgc gcatcttctg caattttttg
     5761 ggcttcttct tcgctgatgc catcgatatg cacttctaat actacaccga tctgatagcc
     5821 gccatcatct aaattataaa gagagacacg tgcttttact gtggactctg ctgtcattcc
     5881 tgcttctttg atcatatgtt gtaccgcccc attgaaacaa gaagcataac ctgctgcaaa
     5941 taattgttct ggatttgttc cttctgcatg gattcctgga ggagtgattt tcatatgcat
     6001 cgagccattt ggtgcctcga cttctccatt gcgcccgcca tggttgatca ttgctgtttc
     6061 gtatgctttt ttcattgtac tgctcctttc gattgaccat ttgctacttg gctataatta
     6121 tagtgtatga caagtaaggc gctttctaca aaaattctgc ttattcgaat accagaatgc
     6181 cgtcttctac tatttttcca aaaaacaaaa aaatactact cgtgaacaac agcgtcatat
     6241 ttgttccaaa ataaatagca atcttttcta cataatcttt tatatgattg attttaagat
     6301 acatatatac cagaaagttg accccataaa ccaatactgt taaaaaaata accatattcg
     6361 ttaccatttt ttcctccttt tgctggtctc cctaccagca ctcgatttaa ccataccact
     6421 ttttttgccg tccaagcaac tagcctttca aaaagactgg tttattctgc tgttaatgat
     6481 tcaagatcag aaaaatgtcc gaataatttt gctaatttta ctaacgccaa cttgttcttt
     6541 ccgaattcat cattttctat ctgtaatact agtaacggct catgtaagct taaacggatc
     6601 aaaaaccagc cgtttccgta tggctcttct agatcatatc gaataccttc ttcattttcc
     6661 gggtcgaagt tcatcccttt ttcttcttcc aacagtcgct tcatttcttc taatatcttg
     6721 tttccttgtt cgcgtggttc atcgctgaac aattttaatc ggatttcttg tacttctacg
     6781 ggctgtttca actctttgat ttgttcatct aatgtgatac cttttctttt taattctggc
     6841 aaaagcatca ggatctttgc gatgacatat gcaccatcat ccagaaaata gttttccttg
     6901 aatgcagcgt gtccgcttgt ttcgatggct agctgtgtat ctttcccaag acggttaagt
     6961 tctaatgctt tattgattac atttcgatag ccggaaatat aacgaacttg gatgcctcct
     7021 ttttcttcaa tgaatctttg tagatgagaa gaagtagggg aattcgtcac gattcttgtt
     7081 cctggatgct cttttaatac gattgtactt aatactgcga tcaaattatt tcgattgatc
     7141 actgttccag aagcactgac aactgcagaa cgatccacat ctgtatcgaa aatgatccca
     7201 agatctgctt catttgctaa aacagcctgc ttgatacttt ccatcgcttc tttattatca
     7261 ggatttggga tatgattagg aaatcggcca tctggttcca aaaattggga acctgttgaa
     7321 tccgcaccta agctattcaa tactttttca gcaaagaatc caccagcacc atttccggca
     7381 tcaacaatga ttttccaacc atttagtggt ttgctttcta gtgttccaat ccccttttta
     7441 atttttgtga ctaaatcttg cgcataaggt gtcaaaaggt ctgctttttc tacgcttcca
     7501 gtttgttcgc tttttatttt ttgctgctgt gataggataa aagcaatatc ttctttttct
     7561 gctcctcctt ctgaggtaaa gaattttatt ccattataat aatagggaag atggctagca
     7621 gtcagcataa ccgcagcatc gcacgagaac tgttcaaatt gagtggacat aaacatagca
     7681 ggcgtagtag ctaattcgaa atcgatgaca tgtatccctt gttctttcag actttggatc
     7741 aacgcctctt tcagtgctgg tccgcttatc ctactgtcat gaccaatccc aatcgtccat
     7801 tttttatttg gatcggttga tttcttcttt ttctttatcc agtttgctag tccccagccg
     7861 atctttttta ccatgtctgt cgtcaagttt acttcatatc cttcaactgg taatgcgatt
     7921 cctcgaatat ccgatccatt ttgcagcgct ttcatatctt tcatataaaa gtcctcctca
     7981 tttattccct cattttttcc agcttttctg caaatcgctg tgcttcttct tctgagcggc
     8041 aaataataat gaatgtatct acaccggcca tcgttccgac aacttcaggg taagccactt
     8101 catctagcca attcgttacc acatcggcac catttcgttc agtcaatacg ataaccatga
     8161 actgaacaga ctggatacgc aaaacctcgc gtctaattgc tgtacgcaaa cgttcttcat
     8221 ttaaagaaac agatggctgg ttaaataatg tatagcgtac tattttgttt tcattttgac
     8281 tttttactaa cttcatttct cgaatgtctc gagaaatcgt tgcttgagtc acatcggcgc
     8341 ctgcttcatg cagccgcgca agcagctctt cctgtgtccc aataggttgt tctgtaatga
     8401 tctggcggat cagtgcttgt cgctgtttct ttttcatatt agacacctct caatgttgat
     8461 tatagcaaaa gataagtaat atttcttcat attttggtat tttttacttt tattttttgt
     8521 tttcattcag gttgctaacc tttttctatt ttaacacgca aaatcgtctt cattttttca
     8581 ggtgctgatt ttcttggatc gctgatgtaa atctctttgt gttctttaga agctctttca
     8641 tagccttctt ttgcgatgaa tgcttctaat ttctcaaacg ttgctggttc ttcggcatag
     8701 ggcccgacgt gtaagatttg ggcacaaaga ccttcttcca tctttttgaa tactagttgt
     8761 tctttcaagt cttccggaag tttgctagaa cgctgaatgg cttcttgtgc tatcgcttct
     8821 gtaacaaagt caggctgtcg gatcatcaat tgataagaaa aatgttcttt tttcatcact
     8881 ggttcatgca aatacttttc ttgcagtccc cattgtcctt ccagtggata gacagtatac
     8941 atctgataat caggaatcag ccaatttttt ttaggcgaca tgcgtattgc atagctgact
     9001 gcatatagac aggctactct tcgtttaaag tcttcctgat tcgggtcgcc gatccctctg
     9061 atgctaaaga atggtttttt aggaacctct agtatttgcg ggcttttttt catactatag
     9121 ctcttgtctt tacgccattc aattttttcc atttttcttc ctccgcgaaa ttccatttag
     9181 ttatttatta tcatacctct ttttgagtga cttggctaat cgtgttgaca ctatttttca
     9241 gtttgtgcta gaataagtga agtaaataaa tcgtacagat aacacgtttc ggcgtgtcga
     9301 gagagaggcg gtttctattt ttataataga aatcactctc tttttttacg aaatttttta
     9361 gaggtgatgt aaatgacaaa acaaaaacag ctgcttttag tacctgcagt aattgcaaca
     9421 gggatgatgt cttttgcggg cgtattgatc gagacggcca tgaatgtaac tttcccaact
     9481 cttatcagac agtttgattt aacgactgca caagtccaat gggtaacaac gatttatctt
     9541 ttgatgattt cgatcattgt tccgctgtct acttatttga atcgcaattt tagtctcaaa
     9601 aaactctttt tagcttcaaa tctgcttttc ttggcaggta taatgatcga tttcttctct
     9661 cctaccttca tggtcctatt gtttggtcga ttgctccaag gtgcagcgac aggtatcgct
     9721 cttccgttga tgttccatat tatcttgaca tttgcccccc tcaatcgacg aggcgcgatg
     9781 atgggtgtcg gtactttgac aacggccatt gctccagcaa ttggccccac ttatggaggc
     9841 attatgactt cacattttac atggaatcat atcttcctgt ttcttgtgcc cgttttgatt
     9901 ctttctttga ttatcggctg gtttgctatt cccgagatgg cagtacaaaa aagtggttat
     9961 ttggattgga taagcgtttt aggaatttgt cttttgttta gtggaagtct aacatttttg
    10021 agtacgttgg gaacagtttc cagctggatt gctttaatca tcggcggtat tggctggttc
    10081 cttttttact atcgaagtaa aaaagtggag catccactga tccgactgtc tattctttca
    10141 aataaaacat tccgcttgtt tttgattggc ttcttagttt atcagttttt attacttggt
    10201 gtatcttttg tcttgccgaa tttccttcag atcgttcaag gaagtagtcc ttttgtcgct
    10261 ggattagcga tgcttcccgg cgcagcggtt ggtgctttgc tttctccttt ttccgggaaa
    10321 atgctggatc aatatggtcc gaagaagcca atttttgctg ggctgattct ttctttgatt
    10381 ggttggctag cattattcct actcatagga cacgcttcag tgggtgtatt gattgcttgt
    10441 catgtgtttt atatgatcgg tatcggatta tcttacagca atatgatgac caccggaatg
    10501 aatgcactga atgaagaact acaaggagac gggaacgcaa tgttcaatac cttgcagcag
    10561 ttttctggcg ctgtggctac ttcattagta gcggtcatta tcaattacgt ccagcatcat
    10621 acttcccata actatgaagt gtctactaca ttaggatcaa aagcagcatt aggcgtcttg
    10681 ttgctgttgc ttctcgtaag ctttgctcga ttcgcctatt atctgttctt tgctaaaaaa
    10741 gcgtagattc catatacggc aggtgttgca tctgccgttt ttttgtgtga aaatggttgt
    10801 ttgtaaaagg aggctttttt atggaaaaaa ttgccattat cggtggcggg atcattggta
    10861 tgactctagc cagttactta gatacatcaa aattcaatgt tgctttattt gatgaaggga
    10921 caggacaggc aactaaagct agcgctggga ttatctcccc ttggttgtcg aaacgcagaa
    10981 acaaacaatg gtatcgtctg gctaaagacg gcgctgcttt tttccctaaa cttataaaag
    11041 atttccaact tgatcaaacg atttacgcac aatcagggac acttttgctt cgacctgaaa
    11101 aagatttagc tgaattggct gttcttgccg agaaacgaaa agaagaagcg ccagaaattg
    11161 gaaacatcga atggctttca cctgaacaga catctcaagc acttccgtta ttgaaaccag
    11221 cagtttcact gcgtatcagt ggtggtggtc gtctggatgg aaaagcatat ttagaacatc
    11281 ttggtcagcg tttagcacaa aaacaaatgg agcagatcca ggcaaaagtc actttgcata
    11341 agaagagcga ttcttggctc atcaagggac cgtttggaga atacacagct gatcgtgtaa
    11401 ttttgacacc tggcccggca cttaagcctt tattacaaga attaggctat gctgcagata
    11461 tacgtccgca aaaagggcag ctcttagcat ttcagacatc ttataagatg agtcaagact
    11521 ggcctgttgc tatgcttgac ggagaagctg atttcattcc tttcatggat ggaaagatcc
    11581 ttttaggtgc tacccatgaa aacgatcaag gctgggattt ggctcctact agcgaagctt
    11641 ataagggatt gactaagagt acagaacagt tcttgactga tccagatcag ataaaaaact
    11701 ggccatacga gttccgtgta ggtacccgtg cttatacgtc agatttttcc ccatttttcg
    11761 gcgtactgcc agatgatcca agcttactgg ctgctagcgg tctaggctct tctggcttga
    11821 ccacaggtcc ttatatcggc tatctattag ccaacttctt aaatactggt cattggtcta
    11881 cggaagatta tcaaaaggac atctccacct atattacgaa aaatccatca tagaaaaaca
    11941 gctttttcaa gtaaggatcc cgagcgatat tgcgcatgga ttacttcttg aaaaagctgt
    12001 tattttagtt caatttctga atcaaattct ccaaactttc ttttgcttca tctggcaaga
    12061 gatcttttcc tatctgagct tcaatgaatc gtaaacgaat atcaagtcgt tgtttcacca
    12121 gatttttata ggacgtatcc tcaaaagaaa caggaaaatc tggtataggt aaatagtgga
    12181 tgtcttcaaa tggaccaaac gacttgaatt ctgccgtttg ttgcacgatt ctttcaacga
    12241 ttgacaatgt cgtttcaggt ttgatttttt tatcatcgaa atatccagta ttcaggacat
    12301 agcaggctgt ttgctgtttc aaaaacaact ctcgaaaagc ttgatagtct tccctaagag
    12361 gatagacacg gaaaggatta gcaaatggtt caatgactag ctcatctagc tgtctttttg
    12421 tagtgatatt ttccgcgtct gatcgttttg ttgctaatgt tgcaccaaaa gacgctgcta
    12481 aaactgggtc attgatcttg ataattggcg gaagcgaatc atctttcatg atccaaaaaa
    12541 cagcatcgat cttttcgcta agatgatcga ctcggtcggg tgtcgcataa cgtgatttga
    12601 ctgtccgccc atttccgttt cgtatatctc ctgttactag gactttttct cctttacttg
    12661 tacatgtcac gcccacattc tgacaagtaa ggaaagaaga aatggccgga tcatcagacg
    12721 gatagtcttg tgttttatca aagtaagaag gttccaaggc agtagcaaaa ccattttttc
    12781 gagaaataac gaaagcatcg tcatgcaaga tcgaaacaga gtgaccttcc gtatgttttg
    12841 ctaaggtaat cgttgatttt ccagaaccgg acaacccaaa tacagccatc gtataggtct
    12901 ggtcattact agcatattgt ttcatcccac cgtggcaggc aataaagcca ttgcgatttc
    12961 ccaatgccca agcaagcgtc aaagtcgctt tttttaattc tccaaagtaa cgtaatccta
    13021 gcacagccgc tgtattatgg agagcatcaa acaaaatgag tccatttgga aatgtttcat
    13081 gtgtccattc gggatcacaa aagatataaa gatctccctc atcaaaggaa acagactttt
    13141 gataagtctc taagatcttc gaattcgctg gctgaaaatt cagcaaataa gaatacaaat
    13201 tgttctcaaa tccttctgga atcagcaaat gactttgcac agaaaattct tcagataggc
    13261 caacgattac ttttgtatgg taaaactggc gcttgcgact ttgatacaca gcttctctta
    13321 aaattttctc gtatttgctt tgttccttgt ctaagtcctc aaagatccgc cgagcacttg
    13381 ctgttcttcc cacaatgctt ccatcattga atactagtgt cctaacatca gctggtaaat
    13441 ctagttcttc agcatgtgag attgttaaat ctgtgatgat cgttcctgga ctcccttttg
    13501 ctagttgata agctgtcttc ctgtcaaaaa cttcatgaac attattaccg taaaatgtcg
    13561 tttctacaat cgatcgaata ctggataaca gtggatttcc acttgtgatt tcgtttaatt
    13621 cgagactctc ttttgtactc atggctcctc ctcattccct aaacaaaaaa acgttttctt
    13681 tattatacat tattcgagtt tttatttgta ctgctgcgct gtgggaagca aacgagttat
    13741 ttttcacaca aaaaaaccga gaacttgaat aaacaagttt ctcggtttca tcattttcct
    13801 tttatcggct tagtaccagc cgtttgctaa ccagaatgtt ttagcagcat cccatgaacc
    13861 ataacgagat gttacatatt gttctgctac acgttcttga ttggcagctg aatagtcgcc
    13921 gtttaagtat gaagcatcta attgataacg gccgatgtaa cgtccgttag tagctgtgta
    13981 tgaaccgcct gattcttttt gtgcgatcca ttcttttgca gagcttgaag ttggcgctgt
    14041 tgcagcagct ggggcagcag gagctacttc aactgtttca acaggtgcaa cgtatgtatg
    14101 ttcttgcgtt gctgtttcag ttgtagatgc tgtattttct gttgttttgt tttctgttgt
    14161 tgctgaagaa tcatttgaag ttgggattgt taattgttca ccaacataaa tgcggttgat
    14221 gtttgcgatc gaattttttt cagcaattgc atcgatcatt gagttgtctc ctgcaaattt
    14281 ttgtgagatc ttagataatg aatcacctga ttttactgta taaacttcat cagcgtgagc
    14341 atttgtagtt cctacaaaca ttgcagtagc accagcagca aaagttgtac ctaa
//
LOCUS       DARDHA010000060        14370 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000060 DARDHA010000000
VERSION     DARDHA010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14370
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.060"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(282..3956)
                     /gene="rpoC"
                     /locus_tag="SDA10_001472"
     CDS             complement(282..3956)
                     /gene="rpoC"
                     /locus_tag="SDA10_001472"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEG4240388.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(4014..7631)
                     /gene="rpoB"
                     /locus_tag="SDA10_001473"
     CDS             complement(4014..7631)
                     /gene="rpoB"
                     /locus_tag="SDA10_001473"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEG4240389.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8359..9327
                     /locus_tag="SDA10_001474"
     CDS             8359..9327
                     /locus_tag="SDA10_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4240390.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9345..10250
                     /locus_tag="SDA10_001475"
     CDS             9345..10250
                     /locus_tag="SDA10_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4240391.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10247..11053
                     /locus_tag="SDA10_001476"
     CDS             10247..11053
                     /locus_tag="SDA10_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4240392.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            11189..12178
                     /locus_tag="SDA10_001477"
     CDS             11189..12178
                     /locus_tag="SDA10_001477"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEG4240393.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12495..13019)
                     /locus_tag="SDA10_001478"
     CDS             complement(12495..13019)
                     /locus_tag="SDA10_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEG4240394.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13306..>14370
                     /gene="scm"
                     /locus_tag="SDA10_001479"
     CDS             13306..>14370
                     /gene="scm"
                     /locus_tag="SDA10_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEG4240395.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         4075 a         3090 c         2820 g         4385 t
ORIGIN      
        1 ctggttttgt tttattcgag gaattattca ataatagcta gcaaaatatt aaaaataacc
       61 gctctatctt cccatcaatt tattttgcat tcaaaaaatg atctatcttt acttttgttg
      121 attgacttct cttggaagat gatcgttccg ataccttaat tggatacgaa ccgatgttgt
      181 taagatcctt tcccactttt cttctagtta gacaaattat tttagacaaa ccaaaaaaga
      241 ggttgtgaca ataatttgtc acaacctctt ttttaaaata tctattttgt ttgattgttc
      301 attgcgtctt ctgcagccat ttgcgcttca atatctgaaa tactgtagac attttcgctt
      361 gctacaccga cttcttttgg ttccatgtca cgataacgtt gcataccagt accagctggg
      421 atgatcttac ctataataac attttctttc aaaccaagaa gaggatcttt cttaccgcgg
      481 atcgcagcat ccgtcaagac acgagttgtt tcttggaatg atgcagcaga taagaaacta
      541 tttgtttcta atgatgcttt tgtgatacct agtaagacag gtcgagaagt tgcaggaacg
      601 cctcctgaga ctagcgtatt gtagttttga tctttaaagt ctgcgatgtc taataatgta
      661 cctggtaaga tatctgtatc acctggatcc attacacgaa ctttacgcag catttgacga
      721 accattactt cgatatgctt gtcgccgatt tctacccctt gcatacggta aacacgttgt
      781 acttcacgaa gcaagtagtt ttcaacagat aatacatcgc gaacttgcaa caattgtttt
      841 ggatcgattg atccttctgt caatggtgca ccacggtgga tcatatcgcc ttctgctact
      901 ttcatacgtg ctgtataagg aacagtatac gtacgtgtat ctgtttttcc tttgattgtt
      961 acttctttgg tacgtgttgc aggatcttct gagatatcga tgacatcccc tgttacttct
     1021 gtgatcactg cttgcccttt cggattacgt gcttcaaaga tttcttggac acgaggaaga
     1081 ccttgagtga tatcgtctcc ggcaacccca cctgtatgga atgtacgcat cgtcaactga
     1141 gtaccaggtt caccgattga ttgagcggcg atcgtaccaa ctgcttctcc gacttcaacg
     1201 tcagagccag ttgcaaggtt gcgtccgtaa caatgtttgc agacaccatg ttttgtatta
     1261 catgtgaaga cagaacggat tgtcactgtt tcgacacctg catcaacgat ttgttttgcg
     1321 atgtcttcgg aaatcaattg gtttcctgca atgatcaatt catttgtttc aggatgacgt
     1381 acttctttac gggtgtaacg tcccactaaa cgatcttcta atgattcgat gatttcattt
     1441 ccctcgcgga tggcttggat ttcaagacct cggtcagtac cgcaatcttc ttcacggatg
     1501 attacatctt gggccacgtc aaccagacga cgagtcaagt aacctgaatc ggcagtcttc
     1561 aaggctgtat cggtcatccc tttacgagcc ccgtgagttg agataaacat ttccaagacg
     1621 gatagtcctt cacggaagtt cgaaatgatt ggcaattcca tgattcgacc attaggagcg
     1681 gccatcaaac cacgcattcc ggcaagctgt gtaaagttgg aaatgttacc acgagctcca
     1741 gaatcagaca tcatgaagat tggatttttc gcttccaaac cttccatcag tttttgttgg
     1801 atgctgtctt ttgcagcatt ccaaacagca atgacacgtt cataacgttc gtcatctgtg
     1861 atcagaccac gacggaattg ttttgtaata gtttcaactt gtttatgggc attttcaatg
     1921 atttcttgtt tttcattcag tacactgatg tcagcgatcc ctactgtgat accggcatgt
     1981 gtcgaatgtt tgtaccctaa gtctttcata cggtcaagca tcttagaagt ttctgttact
     2041 ttgaatcgtt tgaagacttc tgcgatgatg tttcctaagt ttttcttctt gaacggtaat
     2101 actaattctt gctctttgat atgtgcagga atatcagtac cagcttcaac aaagtatttg
     2161 tccggtgttt ggacggtcaa gttgaagtct gttggttcat tcagataagg gaattcagga
     2221 ggcatgatct cgttgaagat gatcttacca actgttgtga tcatcgtacg ttctttttgc
     2281 cattcagtaa atggtttttc acctaatgag ttagggttca caccgatacg tgaatgtaaa
     2341 tgaacatatc cgttgcgcca tgcgataacg gcttcgtttt gatctctgaa gaccattcct
     2401 tcgccttcgc gtccttcttc ttccattgtc aagtagtagt tacctagaac catatcttga
     2461 gaaggagtaa caactggttt accatcttta gggttcaaga tgttttgagc agctaacatc
     2521 aacatacgtg cttctgcttg tgcttcttca ttcaatggaa cgtggacagc catttggtct
     2581 ccatcgaaat cggcattata agcttcacat acaagtggat gcaaacggat tgcgcgtcct
     2641 tgtactaaaa ctggttcgaa tgcttggata ccaagtcggt gaagcgtagg tgcccggttc
     2701 agcaatactg ggtgttcctt gattacgtct tctaaaacat cccatacttc gtcttcttga
     2761 cgttcgattt tacgtttcgc atttttgatg tttgaagcta gttcgcgttg aacaagctca
     2821 cgcatcacga atggtttgaa taattcaatg gccatttctt ttggaagtcc acattggtac
     2881 atcttcaaga aaggtccaac aacgataacg gaacgacctg agtagtcgac acgtttacct
     2941 agcaagtttt gacggaaacg accttgtttc cctttcaaca tatgagaaag agatttcaat
     3001 gggcggttac ctggtccagt aactggacgt ccgcgacgac cgttatcgat caatgcatct
     3061 actgcttctt gcagcatacg tttttcattt tgaacgatga ttccaggtgc attcaagtct
     3121 aacaaacgtt tcaaacggtt attacggttg attacacgac ggtaaagatc attcaagtca
     3181 cttgttgcaa aacgtccacc ttctaattga accattgggc gtaaatctgg tggaataaca
     3241 gggataacgt ccataaccat ccagcttggt tgatttcctg aagcgcggaa tgcttctaaa
     3301 atatccaaac ggcggattgc acgtgtacgt ttttgtcctt gtgcagattt caattcttct
     3361 ttcaattcgg ctgcttctgc atctagatca acattatcaa gtaattgttt aatcgcttct
     3421 gcacccatag cagcttggaa ttcttgtcca tattgttcac gtttttcgcg atattcgcgt
     3481 tctgtcaaca attgtttttt ctctaaattc gtatttcctg gttcgatgac tacatatgaa
     3541 gcaaaataaa tgatttcttc caaagcgcgt gggctcatgt ctaatacaag acccatacgt
     3601 gaaggaatcc ctttaaagta ccagatatgt gaaacaggag ctgctaattc aatatgtccc
     3661 atgcgttcac ggcgaacttt cgaacgagta acttccactc cgcatcgatc acaaacgatt
     3721 cctttataac ggatacgttt gtattttccg caggcacatt cccagtcttt tgtaggacca
     3781 aaaatccgct cacagaacaa cccttcacgt tcaggtttta aggtacgata gttaatcgtc
     3841 tcaggttttt ttacttcacc gtaagaccag cttctgattt tttcgggaga agccaaacct
     3901 atttgcatac tttcgaattt atttacatcg atcaaaaggg gttccctcca tttcatttaa
     3961 tggcaaaccg caagacggct gatcttcgac cgccttgcag ctcatagacc ggattagtct
     4021 aaattatctt ctgcggtttc aaagttttgg atgacatcgt ctttttcatc ttccacttgt
     4081 tcagcagctt tcttttctaa ttctttggca gtttgctgtt cggcaaattt tgtcaaagca
     4141 tcaacagtga tcaaatcgtc gtcttcatcg tccatgtcgc gaagttcgat ctctgtttct
     4201 tcgatatcca agacacgcat gtccagacct agtgattgca attctttcac caatacgcgg
     4261 aatgattccg gtacacctgg ttttggaatt ggttcaccct tgacgattgc ttcgtatgtt
     4321 ttcacacgtc cgactacgtc atctgatttg tatgtcaaga tttcttgtaa agtataagcc
     4381 gcaccgtaag cttccagtgc ccatacttcc atttcaccaa aacgttgtcc accaaattga
     4441 gctttacctc ccaatggttg ttgtgtaacc aatgagtaag gtccgattga acgtgcatgc
     4501 aatttgtcat cgaccatgtg agcaagtttg atcatgtaca tcacaccgac agacacacgt
     4561 ccatcaaatg gttcacctgt acgtccgtcg tacagaacag ttttcgcatc gctggccata
     4621 ccggcttcac gaactgtttc ccatacgtct tcatcacttg caccatcaaa tactggcgtt
     4681 gctacgtgga tacctaactg acgtgcagcc atacccaagt gtaattccaa tacttgcccg
     4741 atgttcatac gtgaaggcac ccctaaagga ttcagcatga tatcgattgg tgttccatca
     4801 ggtaggaatg gcatatcttc ttccggcata atacgggata caaccccttt atttccgtga
     4861 cgaccggcca ttttatcccc ttcatggatt ttacgttttt gaacgatata aacacgaact
     4921 aacatgttta cgcctggtga caattcatct ccagcttcac gagtaaagat tttcacatcg
     4981 tgaacgatac cgccgccgcc atgtggtaca cgtaatgatg tatcgcggac ttcacgagct
     5041 ttttcgccaa agatcgcatg caatagacgt tcttctgcag ataattctgt tacccctttt
     5101 ggcgtgactt tccctacaag caaatcgcca tcttggactt ctgcaccaat acggatgata
     5161 cccatttcgt ctaaatcttt taatgcgtct tccccgacat tagggatttc gcgtgtgatt
     5221 tcttcaggtc ctaattttgt atcgcgggct tctgattcat attcttcaat atggatagaa
     5281 gtgtatacgt catcttttac caaacgacgg ctcatgatga tcgcatcttc atagttgtac
     5341 ccttcccaag tcatgaaacc aactaaaacg ttttggccta gagccatttc gccttgttcc
     5401 atagatggtc cgtctgccag agtatcccct ttttcgactt tctcacccaa atgaacgatt
     5461 ggacgttggt tgtagcttgt tccggagttt gaacgacgga attttgttac atcgtattta
     5521 tctaatgcac cgttgtcgcg gcgtacgcgg atttcagaag catcaacgta ttcaacgaca
     5581 ccgtcatgtt tacataacaa agcagctcct gagtcatgcg ctgatttgta ttccatacca
     5641 gtaccaaccc atggtgcttg aggattgatc aacggcactg cttgacgctg catgttggca
     5701 cccatcaagg cacggttgga gtcatcgttt tccaagaaag gaatacatgc tgtcgcaacg
     5761 gcaactactt gtttaggtga tacgtccatg tagtctactt tatcaataga tacttctaag
     5821 ttttcacttt gagcacgagc cataacaacg tcttgagcaa atgttccatc ttcattcaat
     5881 gggctgtttg cttgggctac gatgtaatgg tcttcgatat cagctgtcaa gtagtcgatt
     5941 tggtcagtaa cacgacctgt ttcacggtct acacgacgat aaggtgtttc gataaatccg
     6001 aatttattta ctttcgcata actggacaaa ctattgatca acccgatgtt tgggccttca
     6061 ggagtttcga tcggacacat acggccatag tgggagtagt gaacgtcacg aacttcataa
     6121 ccggcgcggt cacgagtcaa tccgccaggt cctaaggctg aaagacgacg tttgtgtgtc
     6181 aattcgccaa gagggtttgt ttgatccatg aactgtgaca actgagaaga accaaagaat
     6241 tctttgatac ttgccacaac tggacggata ttgatcaatt gttgaggtgt caatgtttca
     6301 gtatcttgga tcgacattct ttcacgaacc acacgttcca tacgtgctag cccgatacgg
     6361 aattggtttt gtaaaagttc accgactgaa cggatacgac gatttcctaa gtggtcgata
     6421 tcatcgacat tgccgatacc ttccatcaag ttcaagaaat agctcattga agcaatgatg
     6481 tcggctggac gaaccgtttt tacagctgct tctggatagc cgtttccgat cacgtttact
     6541 tcacgttcag ggtctcttgg tgaaaatact ttgatgactt ggacagtcat tggatctgtg
     6601 accacaccgt cttctgatgg atagtaagtc actgaattta ggccattatc aatgaaagga
     6661 gctagtgttt ccatgacttg gtgagtcaga actgttcctt tttcaacgat gatctcgcct
     6721 gtttctggat caactaatgt ttcagctaat gtcagattca atagacgtgt tttcaaatct
     6781 aattttttgt ttactttgta acgaccaacg tttgctaagt cgtaacgttt tggatcgaag
     6841 aaacgagcat tcagcaagtt acgtgagctg tctgctgttt ttggttcgcc tggacgcaaa
     6901 cgttcgtaaa cgtctttcaa accttcttct gttcttgagt cgcttgcgtt tttatgcaag
     6961 tctttttcga ctgtattgcg taatgtttcg ctgtcgccga agatttcaaa gatcgtatca
     7021 tcggaaccaa atcctaacgc acgaactaat acggttaaag gaatcttacg tgtacggtcg
     7081 atacgtacat aagagatgtc ttttgcatca gtttccattt ctagccaagc tccacggtta
     7141 gggataacag tagaaccgaa tccttctttt ccgtttttgt cgacttttcc atggaaatag
     7201 acacctggtg aacggactaa ctgagaaacg attactcgct ctgccccgtt gatgatgaat
     7261 gttccttgtt ctgtcattaa tgggaaatca ccgaagaata cttcttgtgc tttgatttcg
     7321 ccagtctcgc ggtttgtcaa acgcaatgtt acatgtaatg gtgcagagta gtttgcatca
     7381 tgcgcacgag cttctgctac tgtatacttt ggttctttca attcataatc tacaaattcc
     7441 aaagatagat tgccattgaa gtcatcgatt ggcaaaatgt cttcaaacat ttctctaagt
     7501 ccctcatcta agaaccattg gtaagagtct gtttgaattt cgatcaaatt tggtaattcc
     7561 aacacttcac tgattcttgc gaaacttcta cgttcgcgat gttttccgta ttttactacg
     7621 tgtccagcca agctcttcac ccctctaaaa tgtcattcaa aaagactacc cgatcaattg
     7681 ttacaataat atttcaaata ttatttttag gtaacaacag acattttttg cggcttttag
     7741 gcaaaaaaaa accaaaaata gaacattctc ttgaaacctt acttcgtgag cttctacttt
     7801 tgtttcctta tattaaagcc gaaacggaca atatttcgtt tctgcctttt cttcatcaga
     7861 tagtttttgc aacttcatat actacactta ttttctataa aagtcaacac ttcctggcgt
     7921 ttcttctgct ttttcgcaac attttcttaa tgttttcaat tttatgtttt caattatctg
     7981 acaattctta attgacagac ctttttatct cggttatact ttagaaaatt cattttgtgg
     8041 aggaacttct atgcgaacca acatcaacca aatcgcacca acacaactga ataactttta
     8101 ctttagctac tttagatagg tttgtattca tcatgatgga tacaaacgtt aaacgacagt
     8161 ttgtatctat gatggctaaa gaactttgta ttgcagcaaa aagccacgta gacaaaatgc
     8221 taagtggctg agcagtgaaa atacaaagta aaagtatcaa gcattctact ttttcaattt
     8281 attttccgac ttttcaatcc atttggacat tttgtctgaa tggatttttt gtttcccaaa
     8341 attaaggagg aaaaagaaat gaaaaaaaat ataatcatcg gattaggcgc tgccctgcta
     8401 ctagctttag gaggttgcgc acaaaaagac aattccgcag aggcatcaga tggacaaaaa
     8461 acaattggcg tattgcaact tgtacaacat aactcgttag atgctgccta taaaggattc
     8521 aaagaaggat tagcagaaga agggtataaa gaaggcgata atctaacaat cgattatcaa
     8581 aatgcccaaa acaatcaaga caacctgaaa agtatgagtg agaaactcgt caaagaagat
     8641 cctgatttgc tacttggaat cgccactcca gcagcacaat ctttattaaa tgaaacaacc
     8701 actatcccga tgactgtcac agcggtcaca gacttggaac aagcccattt agtcacatcc
     8761 aatgaaaaac ctggcggcaa tgtgactggc acgacagata tggtgccaat cgaaaaacaa
     8821 atcggcttac tgttaaatat cgtgccagac gccaaaacaa tcggcatcat gtataacaac
     8881 ggtgaagcaa actctaagat ccaagctgat ttggcagaaa aagaattgaa aaaagcgggc
     8941 gtcaaagtca aagtactgac agcaaattcc acaaatgatg tccaacaagt gacgaaaagt
     9001 ctagctaaag atgtagatgg aatctatatt cctactgata acacgttcgc aagtgcggca
     9061 agtgttgtcg gtgaagtcgc aaaagaaaca aaaacacctg tcgttgctgg cgcagttgat
     9121 caagtcaaag acggcggtct agcaacagta ggaatcgact acgaaagctt gggcaaacaa
     9181 acagggatca tggcagcaaa aatcttagac ggcaagaatc caagtgaact tcctgtcgaa
     9241 acagcgaatg atttggaact gtatgtcaat aaagacatgg ccaaagcatt aggcatcaat
     9301 ccagacagca tcaaagaagc tgaataaaac acaggaggat tctcatgaca aatttgctct
     9361 ctcttttctt atcatctgca tcgcaagggg tcttatgggc cctacttgcg attggtgtat
     9421 acgtcacttt ccgtatcttg gacattgccg acttgactgc tgaaggaagc tttcctttag
     9481 gtgcagggat cgcggcagta agtattacaa atggctatca tccattgaca gcttgtcttc
     9541 taggcttttt agggggtgcg gcagcagggc ttgtttccgg actgcttcac acaaaattga
     9601 aaattcccgc ccttcttgca gggatcatca ccatgactgg tttatattct gtcacatccc
     9661 gtgtgatggg tgcgccgaac atctctttat tgggtcagcc aacgatcttt cattgggtcg
     9721 agtcattcgg cataacaaaa gacaacgctg tactgatcgt cggtctgctt tttgctttga
     9781 tcgttgtatg tttacttgtt ttgttcttac gtacagaaac aggacttgct attcgtgcaa
     9841 ctggcgataa tctggcaatg agcgaagcaa acgggatcaa cacagataat atgaagatca
     9901 tcggttatat gatttctaat ggatgcattg ctctttctgg ctccttactt gcgcaaaaca
     9961 acggctttgc tgatttaaat tctggtattg gaacgatcgt aattggactt gcttctatta
    10021 tcatcgcaga agttttgtta cgtaaccagc cactatttct ccgtctcttg actgtcgttt
    10081 taggtgcggt catttatcgt tttattttag cgttagtctt cgaactgaat gttgaaccaa
    10141 gtgattcgaa acttgcttct gctttcgtgc tagtgatctg tctttctttc ccgcagatcc
    10201 agcaaaaatt ccatttacct caaaaaaaga aaggcggtag tgtcaaatga atgacgtctt
    10261 aacattggaa aatctccatc atacctttga aaaaggaacg atcaacgaaa atcatgtcct
    10321 gaaaggcatt aatctttcct tagatcccgg cgacttcgtg acgatcatcg gtggaaatgg
    10381 ggctggtaaa tctactttgc tgaacagcat cgccggtaca ttttctgtcg atcagggtca
    10441 aattttattg aatggaaaaa atatcacgaa aaaatctgtt gtcgaacgtt cgaaatccat
    10501 cagtcgcgta ttccaagatc ctaaactagg gacagccgtt cgtttaacgg tagaagaaaa
    10561 tcttgctctt gctatgaaac gaggaaaaaa acgcggcttc ttccgtggtg tcaaaccaca
    10621 agaccgaaca tttttcaaag accagctggt tcggctaaat ttggggttgg aaaatcgttt
    10681 atcctcagaa atcggtttgc tgtctggagg acaacgtcaa gctattacgt tattgatggc
    10741 cacattacag cgtccagaac tgatcttatt agatgaacac actgcagcgc ttgaccctgc
    10801 tacttccaaa acggtgatgg aattgacaga aaagctgatc actgagcaga atctcactgc
    10861 ttttatggtc actcatgaca tggaagacgc gatccgctat gggagtcgac tgatcatgct
    10921 gcatcaagga aggatcgtcg tagatatcca gcaagaggaa aaacagcagc tcacagtgcc
    10981 ccaattatta gcgttgttca aacaaaacag cggcacggca ttaaaagacg atcaagtact
    11041 tttatcttca taatcagaaa gaggatggga caaaaaatga acgcccaccc tcttcttttt
    11101 tctctttcct tctttcgttt ttgctttatg tatcaaccgt tgtttcttcg aaaactattt
    11161 tgttctctcc ttcttcctga tagaatggat gggaaaggag cttgatgcca tgacaacgaa
    11221 agacaaggta cttcaattat tgaaaaagac accagatttt ctttccggag aaaagttggc
    11281 acagcaactg gaagtttccc ggacaagtat ttggaaagcc atcaaagaat tggaaaaggc
    11341 aggctatcgt tttgagcacg ggcctacagg gtatcgttac ctgccctctg acgtactaga
    11401 tgcaaaagaa atccagcaag agctacggtc gtttattcct gatttgaaag tacaaacgat
    11461 gttgacttct gaatctacga tgaaagatgc caaactggca gcagctcaag gagattctgt
    11521 ttcgaagtta tttgttgctg atacacaaga acaagctcgt ggacgttttg gacggccttt
    11581 tttcgctgaa cctggacgag gaatctacat gagtctcttg ctccatccag acaaacggtt
    11641 tgatgaattg cctcaatata cgatcatcgc agccagtgcc tgtgtgaaag ccattgaatc
    11701 tgtaaccggt cgttctgctt cgatcaaatg ggtcaatgat atctacatgg atggaaaaaa
    11761 agtctgtggg attttatctg aagcagtcag tgatatggaa agcggcagga tcagtcatat
    11821 catcattggg atcgggctta atttttctgt tcctcaaaat cattttccag ctgatctcca
    11881 agaaaaagcc atctctgtct ttcccgacgg acaagctagt gtcacaagga atcagctaat
    11941 cagcaaaatc tggcaagaat tgttcactta tctttctcac ccagaaaaag cgttggactt
    12001 ctataaagaa aaatcattcg tattgggaaa aaccgtgtcg ttcaatcaaa aaggaacgac
    12061 ttatgtcggt cttgcaaaag aaatcggtga tcatggcgaa ctgatcgtcc aaacaaatga
    12121 acagaagatg agtttgtctt ctggcgaaat cagtctcaca tctatccgta cgcaataatt
    12181 atcagatttc acaaaaggac acctgatctc atacagtgtc cttttttctg aattctcttc
    12241 aaaagactct ccttattcta tcagctgaca gtttctctat cctctagaaa aaggtattct
    12301 aaaaatatct ataaagcatg gtacactttc tttaaatgaa tcaatgcaaa aaacaagaga
    12361 gtcatcagtt tacacgatca aaagagatag caggccgctt cctttttcaa tcttatagat
    12421 agaccatggg taacgataaa aacaaaaaag aacttgcgac ttattttgcc gtaagttctt
    12481 ttttagctag tgccttaatc tcttaatccc aacaaacgtc tcgcatagat cacctgacca
    12541 gagtgcatag cagcatcatc aataatagaa acgagacgca ttcctcgtgt gactggtggt
    12601 gtccagttct cgtcaattac ttcatctagc tcggaaggtt ccagccttct taagtaagaa
    12661 atagcaaaat cagcagctcc tcgcaagtat cctagcagat cttctatttg attagcttgg
    12721 atctgctttg cttgttccag actatgcttc caatcttgtt cgttatctgc tatcgtcatc
    12781 ggaaaccggc tttcccagtc ttgactatgc caaattggag aagttcctgc taaatcagca
    12841 atctggaaat ctaattctcg agcggtatgc caagccagcc aagtcattga tttgatctgc
    12901 ggggctctat cttccactgg aaaacgattg gcctcctcaa tagagacgcc ttcaaacaaa
    12961 cgttcaaacc gatcgtatgc ccgatccaca ctctcgcata gcatatctaa ataatccatt
    13021 tattttcctc ctttttattt atcctactta ctttttccta attctaggat acctacttat
    13081 atgttactac acacccttat tttttacact ttaacagtca ccatcatcaa tagtttggag
    13141 attgaaagaa aaagatagtg tcgaactatc aattgaacaa gaagcaacaa tatgaacgtt
    13201 tcattaatat tctttaaata aacaatgatt tttcaaacga acagtttaag actaggagaa
    13261 aaagagtata ataggaaaat gtttgaaagg aaaggaaggg gaaaaatgaa aaaaagggca
    13321 agtttactag tcctagttgc tttgttgttc aatatctttt cgtatggtgt ggccgtttca
    13381 gcggaaacat acattcctaa agaccctggg aatatttata atgatctaac tggtaactat
    13441 ggcttgttag gaattgctag tcaattccac gtatttgcta aaaataagac aactgtgaat
    13501 gcacatacag acggaaatat agcaacacat gaacttgatg caaaaaataa ttttggtaca
    13561 gagcttcatg aaggtttatt gaaccaagaa ataaattata tacaaaaaac tgattctttc
    13621 gctaattctt caggcatacc tacaggtagc acaagaatga ataaatttgt cgttggcagt
    13681 aacgtggatg tagcacttca agatggtcat cctacagtca atggatccag gcttgatcac
    13741 ttaactccag atgattttta taaagatcgt gctgggcatg aatatattga tttcgagcag
    13801 gaatttgcta aattgaatgt agcagcaaat gatcttgcaa cgatcactcc agctaaaaca
    13861 tatactgctg cagattttcc agacatgaac aatcggacaa tcgatttaac tgatttatca
    13921 gataccgggt ttttattggt aaatatcgat gcagaagtgt taacgatgaa tacgccactt
    13981 caaatcatta atccaaatga tcaagtagtt gtatttaatg tcatcaacag ttcttctgca
    14041 ttgaacgtac agtcaccaat caaatacaat gatcgttcaa atcatgaaac agaagatttt
    14101 tcggatgcga atatctcttg gaattttgga aatgagatga ctgatttgac aatcagtgct
    14161 ccttttcaag gaacaatcct tgcgcctaat gctactatcc gcattactca aaaccaagac
    14221 ggaacgatta taggaacaaa tgtgatttta gatgcagcta ccaatcgttg ggatcctaac
    14281 gaaatattta tcagcgacaa tggtacagat acgacagaca caagcgacag tacagataca
    14341 tctaccacaa gtgatagctc agattcatca
//
LOCUS       DARDHA010000061        14243 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000061 DARDHA010000000
VERSION     DARDHA010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.061"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..527)
                     /locus_tag="SDA10_001480"
     CDS             complement(<1..527)
                     /locus_tag="SDA10_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG4240396.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNK"
     gene            complement(775..1611)
                     /locus_tag="SDA10_001481"
     CDS             complement(775..1611)
                     /locus_tag="SDA10_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEG4240397.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1622..2551)
                     /locus_tag="SDA10_001482"
     CDS             complement(1622..2551)
                     /locus_tag="SDA10_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEG4240398.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2743..3096
                     /locus_tag="SDA10_001483"
     CDS             2743..3096
                     /locus_tag="SDA10_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG4240399.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3170..3826)
                     /locus_tag="SDA10_001484"
     CDS             complement(3170..3826)
                     /locus_tag="SDA10_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEG4240400.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            4052..5986
                     /locus_tag="SDA10_001485"
     CDS             4052..5986
                     /locus_tag="SDA10_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG4240401.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(6072..7295)
                     /locus_tag="SDA10_001486"
     CDS             complement(6072..7295)
                     /locus_tag="SDA10_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG4240402.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7289..9187)
                     /locus_tag="SDA10_001487"
     CDS             complement(7289..9187)
                     /locus_tag="SDA10_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEG4240403.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(9181..10299)
                     /locus_tag="SDA10_001488"
     CDS             complement(9181..10299)
                     /locus_tag="SDA10_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG4240404.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10382..11215)
                     /locus_tag="SDA10_001489"
     CDS             complement(10382..11215)
                     /locus_tag="SDA10_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4240405.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11208..12125)
                     /locus_tag="SDA10_001490"
     CDS             complement(11208..12125)
                     /locus_tag="SDA10_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4240406.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12148..12327)
                     /locus_tag="SDA10_001491"
     CDS             complement(12148..12327)
                     /locus_tag="SDA10_001491"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240407.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(12352..13476)
                     /locus_tag="SDA10_001492"
     CDS             complement(12352..13476)
                     /locus_tag="SDA10_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG4240408.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            13659..>14243
                     /locus_tag="SDA10_001493"
     CDS             13659..>14243
                     /locus_tag="SDA10_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEG4240409.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHIL"
BASE COUNT         4482 a         2831 c         2303 g         4627 t
ORIGIN      
        1 attttatttt tataacctgg catgctcatt agataaagga tttcatcgta atggatatag
       61 tgatcgctat taccataacg gacagttgtc cacctttttt tcgtagcatg ttgaacagga
      121 tagttttctt tttctttcaa taagtaactt tggatgcttt ttttattttt ttcataagtg
      181 aattctttta tcaaataacc actaggcgta atctttcgat taataacagt tattgcttta
      241 ttcggatgag acgttagata aacaatttta cacttttcat tcttttctct aagcatttcg
      301 cctagttcga taccgctata ataggcgttc aaatcgatgt ctaggaaaaa aatatctgag
      361 gaatagattt cattattttt aatgaattca acaaattgta taacgttcaa tccctgaacg
      421 atctcaaatt tccaaggact taataaaatt ctggattctt ccaaaatttc tactagcatt
      481 ttttcaaaaa ccaaactatc atccaaaata aatatacgcg ttaccataaa aagtacctcc
      541 actcgatatt ttactatcaa aatcatttca tacaactttt caacatacat gttaggaata
      601 aattgtaatt ctcttcacaa tcttttccaa cacctttgaa accgatatca aatactttca
      661 ggatgctgct tctaaaataa atatcaaaca aatgaataca tcatgcaaaa aaagccagac
      721 aaccgacgtc cttgcgtgtc agctgtctga gctatgttta tttattttgt tcttttaaaa
      781 agaacgatac acatactgat ttttcggagc agagactttc tcagcgtttg taacaactac
      841 agatgggatt tcacgcttga atggttgata atagtctgat tggatggttc cttctatttt
      901 ataccattca tcatttttaa ccgttactcc ttccggcata tgcgtcaaca aaccaaagac
      961 tcctgaatct gccacacaat ggataatgcc gaatcggaac aagaagatat ctgtattgct
     1021 atcgttcggt gtttggtaaa caaatccttc atagctgatt tttttgccaa tgaattcgct
     1081 agggtaatta taaattagct ccataacttc taaatagttt tcatctgtga tcttgatagt
     1141 ttgcttatcc ccatactttt ctttcaactc attcatttgg tctaagtagt ctgatttgtt
     1201 gaaataaata ctggtatccg gctttaagta ttgtgtctgc atctccggat cccctactga
     1261 ttctttactt actgggaaat taaatccttt ggcttctacg atcgtagtgt cgagactaac
     1321 tgttggaaac aacgtcccca cgattactgg caaagctagc agtacataag cgattccacg
     1381 ttgataaggt tttgacaacc catggtcatg atcgtgctcg tgtgcctttt cttctgcttt
     1441 tccgtttttg acccacaaat acagctgaac taaagccaaa agagcagata aaatcattga
     1501 aagaatagct aagtagcgat aatggacatt gatatattga tcgagtttgc cagaaacttg
     1561 caagtacatc atcaattcga aatacccaga taaaatcaaa aaccgcagca tattttccct
     1621 cctaaataaa taatgtgaat ccaataacgc taagggcaat cagtccgatc agcgataaaa
     1681 taaagcgtcc tgtaaaatat cgtttcatca tcagtagatt tttgatatcg accattgggc
     1741 caaagaccaa aaaggcagct acaggtgctg tgccgaacag acttaacaaa gaagatccta
     1801 taaacgcatc tgcttctgag caaagagaca tcgtaaaggc caataacagc atgatgataa
     1861 tggctaaaat ctttgttgag ccgagcgtta gcataattct agttggtaaa taagtctgca
     1921 tcgcagctgc gattaatccg ccaatgatca aataacgacc tgtatcaaaa aattcgtcga
     1981 ttgaatgtgt caacacatgc ccagtttgat ggatcagctg attttttggt tcattcttct
     2041 cactttgttc ttcttcatgg acatgatgat ggtgattttc tgctacttta gacgtgattt
     2101 tttctttcaa tacagcgtct ttttgaaaat aggcaagcca gacaccaatc acaagggcaa
     2161 caaaaaagct gcctatcacc cgccagcttg ccatctgcca agaattgcca aatgcgataa
     2221 aagtggaaaa aatcacgatt ggattgatga tcggagcagt cagcataaaa gcaaacgctg
     2281 tgtgaactgg tacgcctttt ttaacaaatt gatgaacgat cggtacgatg ccgcattcac
     2341 atgaaggaaa gaaaaatcct aagatcgaac cagtaaaaat agagaagata cgattttcag
     2401 gaagccatct cttcacacgt tctggggtca aaaatacttg taaggcgcct gagataagac
     2461 agcctaataa tacgaaaggt aatgcttcaa taacgatcga taagaaaatc gtactcatct
     2521 gcaaaaccga atttggaata aaagaaaaca tgagtttcct cctttttatt tctagttcat
     2581 tcgctttgca aatatacgct tttattcaaa aaaaacaagt tgctctgtaa atttttacac
     2641 aactcttatt ttttaccaaa gcacttcaat tgactttttc tgttattccc attaaattaa
     2701 ggtctcatac atatcacttc tatcataaaa gggagaataa aaatgaaaca aaccactaac
     2761 ttgactgaag ttcaagacat actgcaacag tcatccgttg ccgtcattta tctttcgatg
     2821 cctaattgca gtgtttgtca cgctgttcgt ccacgactag aggaacttct cacacattat
     2881 gatattccgg cacttcattt ggatgcattt gaaacaccag aagtagctag taaatttgaa
     2941 gtgctgactg cgccagttgt cctgattttt catcaaggaa aagaagtctt tcggcaagca
     3001 cggttcatcg actttaaaaa aatccgttat ctactagatg aactgacagc agaagatgat
     3061 tccctttctt atgaagaaat cttccgaaca aaataatcgt tgtccaaaca gaaaaaagcg
     3121 attgaacaag tgaaagtttt gccttcattt gttcaatcgc tttttatttt tattctgctg
     3181 tattttcgga atgcgcattt gcgtcagaat gcaagaaata gatcaaggtt tgcaattcat
     3241 tcgtcaaatc aacattttgg atcaatacat ctgacggagc attcaatcga gcagcggtaa
     3301 agttcaagat tcctttgatt cctgcttccg ttaactgatt gactacttct tgtgctcttt
     3361 cagcgggtat cgttaaaatt gctacttcga tttgttggat cttgatttgt tctgacatat
     3421 cttccattgg ataaacgggg ataccgtcta cgatgcgccc aacgatttcc tcattcacat
     3481 caaaagcaca actgatacgt atgctattac tttgatggaa tttgtatttt agtaaagcac
     3541 ttcccaagtt ccctacaccg atcagcgcaa catttgtcaa ttgatcgtcg tttaatgtct
     3601 ttgcaaaaaa gtgcataaga tcttctacat cataaccata cccacgtttt cctaattcgc
     3661 caaaataaga aaaatctcga cggatcgttg cactatctac ttgaacggct tcacttaatt
     3721 cagttgaaga cacttttttc tttccagcat tatttaaaat ccttaaatag cgataataca
     3781 aaggcagtct tttagctgtc gcctttggaa taatatgatc tttcacgaaa tgacctccac
     3841 gtattgtcta attacatctt tgatttctcc atcatagcag tatttatagg aaaaaatcaa
     3901 ttccattgct cagatattgt gaaattttat tctttattca tttatttatc atttctgcaa
     3961 atctcgcagt ataatggaat ttatggtaga ctactaattg aaaaatagtt ttttatttga
     4021 ggagaattca caaagtagaa aagaggattc catgatatta ttacaagcca atcagattgc
     4081 tcgccatttt ggtgcagaag tcttatttga aaacatacat ttagaaatca gtacaggcgc
     4141 tcgtgtcgct ttagttggca gaaatggcgc tggaaaatcg actttattaa agatcattgc
     4201 tggaattgaa gcacctgatg aaggaaagat cgctaaaaat aaaacagcga ctctcggata
     4261 cctcgctcag gacacaggac ttgattccaa tgaaacagtt tggaacgaga tgctgaaagc
     4321 ttttgaacaa gttagacaaa tggaagagcg gatgcgcgag ctagagattt tcattagcga
     4381 aggtacgcca gacacacctg cttatagttc ttatttaaaa gaatacgacc ggctgcaaca
     4441 cgatttttca gatcgaaacg gctatggtta tgaaaatgaa attcgttccg tattgcatgg
     4501 gtttaaattc gattcttcat tttatgatca gaaaatcgat actttatcag gcggacagcg
     4561 cacacgttta gctttagctc gcatgctttt gcaacaccct gacattttga ttctagatga
     4621 accaacgaac catttagata tcgagacact ttcctggctt gagaattatc tgcaaagcta
     4681 tactggtgca ttattgattg tttcccatga ccgttatttt ttagataaag tagtcaatga
     4741 agtctatgaa ttaagcagaa gaaaaatgcg ccattacaaa ggtaactatt cgaaatatct
     4801 agatttgaaa gcagaacagt tagcaagtga atggaaggct tatgaaaagc agcaaacaga
     4861 aatccataaa ttagaagact tcgttgctcg gaaccttgtt cgcgcctcta ccacaaaacg
     4921 agcgcaaagc cgcagaaaga cattagaaaa aatggaccgg ctggatcgtc ctcaagggga
     4981 tgaaaaacaa gcaaactttc tattcagtat agaaaagcct tctggaaatg tagtcttgca
     5041 aatcgaagat gccgctatcg gatatgataa tgtcacctta tctgaaccaa tcaatctaga
     5101 tatccgtcgt caagaagcga ttgctctagt aggtccgaat ggtatcggga aatcgacgtt
     5161 gctgaaatca atcattcacc agatcccttt tatccgtgga aaagaaacgc tagggacaaa
     5221 tgtcaccatc ggctattacg atcaaggtca agctgatctg catacaaaca agacgatcct
     5281 agcagaattg tgggatgagc atcctaccac tccagaaaaa gagatccgaa gtgctttagg
     5341 tgctttctta ttttctggcg aagatgtaga aaaaccaatc cctctgctaa gtggcggtga
     5401 aaaagcacgg gttgctttag ccaagttagc gatgaacaaa gaaaattttt tgattttgga
     5461 tgaaccaaca aaccatttag atattgacaa caaagaagta ttagaaaatg cattgatcga
     5521 ttatgacggc acactgcttt ttgtttccca tgaccgatat ttcatcaatc ggatcgcgac
     5581 aaaagtcgtc gaactctctg aaaatggcag caaagtctat ctaggagact atgattatta
     5641 tttagagaaa aagcaggaag aagaagaaat cgccgaattg ttggcacaag atgagccaga
     5701 atctgctcct gctcccacga acaaatctga ttattaccaa agcaaagaac agcaaaagct
     5761 tatccgtagc cttcaacgaa aaattactca ggttgaagaa gaaatggcgc ggatcgatga
     5821 atggatcgat cagttggaac aagaaatggt cgcgccagaa aacctggaag atcacgtaaa
     5881 actcaatgag ctgaaccaag atttagaagc tgctagacaa gatcaagaaa caaaattgac
     5941 ggaatgggaa gaactaagct tggaattaga agaatatgaa aattaacgaa aaaaccaaaa
     6001 cagaaaccat tcatgctgtt ttggtttttc tttttttatt ttatcaaaca aaaattccat
     6061 atttaccttt ttcaccaata agaagaccaa ttactagtca ttctcactaa agcttctaaa
     6121 taatagtaat caccccaaat acaaggtgta tttaccccat tatttttggg cttattataa
     6181 acagactgtg ttaaaaagcc atcttcttct tccgttgtag catatctttc tttcaattgt
     6241 cgcataattt tccaaacagc agactggtat aagttctttt ctgttttgtc taatagacct
     6301 gattctatta attctaataa tccacaaacc atgatcacca tactagatgt atctttttct
     6361 tctctgctag aatcatcaaa gatcaaatcc caataacaaa tggtatcttc tggcaaccga
     6421 ttcataaaat aattggctaa tattttgctt aaatcaaaat atttttgttc tttcaaataa
     6481 cgataactta atgcaaaacc gtaaatcccc catgcttggc ctctagccca acaggaatta
     6541 tcactataac cttgatgcgt acttccatat cttggttttc catctttgac atccatataa
     6601 aatgtatgat aagttgaacc atcttctctt actaaataat ctgctgcatt ttgaatatga
     6661 gcctgagcag cttttttata tttttgattt ccagtttctt cactcgccca atacaataat
     6721 ggaagattca tgcaacaatc catgatcatt ctaccttgtt gtgaaggatc actaggatct
     6781 ccccaagctt gtataactcc tgccttctcc caaaaacgct gaagtaatgt ttctgcagca
     6841 tcaattgaag cttccttcat ttttcgattt cccgttattt tataatctgc tacggttgat
     6901 aaagaataca aaaaaccaag atcgtgatga ttagtgataa tttgacagtc aatcctgtgg
     6961 ataaaatcta tatctgcttt ttcagctgtt tgttgaaata aaggatcacc cgtcttctca
     7021 aataacaacc aattaattcc tgtccaaaat ccttctgtcc aaccaatatt gtctcttaat
     7081 ggataacgtc cttttatagg acactcgctt gggaattgat actgactgtt actgatacgt
     7141 ttgattaacc tctccgtgtg tctgcgacaa cgctcgatct cattattcca ttctttttcc
     7201 attaattctt tataacattc atttatccat ttttctggat gtattatctc ttcaaacatc
     7261 actttatcaa gcatttcccc accagccttt agcataattt tagtcttttt ttattgtttt
     7321 tattttggat agttagcccg gttgtcccat caaaatcaat tttgggacga ttgttccata
     7381 cttctaaact ataatcccga tcttgatgag caaccactcc tgttccgagc caatgggttc
     7441 cttttccaaa tgtaccctct aatccaggaa taaaagtgtt ttcccatgtc atcaagtttg
     7501 tattgggcaa acccataata gctgtggatt ttcgtttaat agtatcgtca cttaaacaga
     7561 taatacctga aaagccagct tcactcaccc tatagttttc cttgtcttct tctatgatta
     7621 ttccttcttt ctcaggagaa cattcaatag caaaccctgt ctcatataga acatattctg
     7681 tatctaattc tactttgtgt acgcgaatct gccagccttc aaaaggaata agccaagttc
     7741 tgatttttgc ctgtaaagta ggttcccatg tagaggcaag tacctctttc ttacatacaa
     7801 tgtctcgtac attatttcga acgatgaact gtccataggt tttgatgctc tcattcttaa
     7861 aactggtcgt gatatatgtg tccgctcgct gaatccctag cattgaatcc attgccattt
     7921 ctgctttcaa ttgattcgct cgcggtatcg aaaaaccaaa ttgactagaa taagcaaatt
     7981 ttaaatattt ttcattggtc aatgtatgga aatttggccc taattgtccc gcattcaaaa
     8041 aaaatgtatg tccattatca tgttgtatga gcaaattggc ttctgaaaga agtttagttc
     8101 cttcacgaat aggcataggc atttctttag ccgtccaata ctcatggtct tcaggtagcg
     8161 ccaacaataa aaaaatctta ttcagccata atggagataa cgtactatta tagggttcag
     8221 tcaatgtcaa ttgagggtag gtataaccaa tattcaggat acctttttca tcaaatattt
     8281 ctttttgcat ccaccatctt atattccgat tgactaaccc cttgatttca cctaatttaa
     8341 aaggcaaaag atcggcccac aacatagctg accaaaagct tgaaactgca aaacgataaa
     8401 tcatgcttcg accaaaaggc acatttgcac catcttccgc aaaaaaatga atatagtctc
     8461 tagaaaaatc aatagctcgg ttcttatata tctggctaat gggcttttct ggttctagta
     8521 cgctataaat caaaccataa taatgaaatg caaaaggatt ataataatct tggcgacctc
     8581 ttgaactgtc tcgataccag ccattttgaa gataacaatt ttctatatta tcaaatgctt
     8641 ctttcagtgc tgattcttta actggataac caaatttttg gataattgtt ccgacaatta
     8701 tcttaaaaaa ttgccaatta ccatctgcac attcatattc aaaaatttgc gagaaccaat
     8761 gaataatctg gcttttttct cctgtatcta attttttcca taataattgt tcatgatgat
     8821 ataacgctaa taacattgcg ggcatttcta ccatacgctg gtctctttca tgtatttctc
     8881 cccaataatc gggatgggaa ggatctgttc cagcttttat tcctgataga attaatttca
     8941 accatcgttc atctaattgg tcaattgcgg gccccgctcc ccataaaacc cgagagaatc
     9001 cttccattcc tgcaatttta tttccatatc ctaccccttg atgtatataa tcaatttgtg
     9061 cgccacttgc tcgataaaac ggctggagtg gttgatttat ctttttaaat aagtcaaagt
     9121 aatcctgtct ggtccttaat agtggtactg gatgtttcat tgtattttct cctcttttta
     9181 ctaccaaata gctaattccg actgttctaa tcgaagtaag ccttcaataa aaaaataatc
     9241 agaatagatc agtggcacat tataagtcag ttctctatgg tatgcttctg ctccgcctcc
     9301 aatgatccca tcttcatcca tattccaatt tgcatagtgc ttttctgtct tttggagtat
     9361 attgatagca gcttcatgat acatttttga tcgtacagag gaaacatatc ctgctaattc
     9421 cagaagaccg catgcagcgc atactccagc actggtatca atattcgaat cttttacagc
     9481 aaaatcaatg agaggaatat aaccagtttt ttcgattttc tgaataaaat ttactgctat
     9541 cttttctgct atagctagat agtacggatt ttttgtgtaa cgataactta acgcgaatcc
     9601 atataaagcc catgcatttc ctctgctcca acaagaattc tcattgtatc cttgcccgcc
     9661 aagtaaccga atatatttcc ctgattctgc atcaaattct gcgatatgcc cggttgatcc
     9721 atctttcctc actagatact tagctgttgt atctgcatgc tgccgagcaa ttttagagaa
     9781 cctcggatca ccagtttccc tcgaagccca atacagtaat ggtaaattca tcatactatc
     9841 aataatcatc caaccaggtt tatggtcatt ccatgctact aaatagttcc ctgaaatatt
     9901 atagcgtcca gcaagtaccg tagctgcatg taaccctagt tgtcgactat gtttgttttg
     9961 agactgttga taatgtgcaa tagccgatgg taagaataaa aaacctacat catgatgaac
    10021 gtctgaaaaa tggtacaggg cagattctaa taattgttct tgttgtattg ctgcctcttt
    10081 gaacttttct cgttttgtct tgtgaaagag ctgccataaa ataccactcc aaaaaccatt
    10141 ggtccaccag tcgattcctc tgtcctttat tacgtctgta tattttccgt tgtaagaaat
    10201 atatggaatt ttattcccca ttctgtctaa ttccgcatct atcttttcag aagctacatt
    10261 caaaattttt cttgaccatt ccttcgtctg ttcattcatg gatatcccgc cattctttta
    10321 atttttatga atgtggagtt gctcctagaa cgaactagat ggttaacatt cgttattttt
    10381 tttacttatt tgcagctcca atgtcattct ccttaaattt agactgcgcc cacacataaa
    10441 tcaagaccat tgggatcact gaagttgcta aaattagcat aattgcgcca taattaattg
    10501 gctcacctgt actagaaacc gtcatatttt tgataattaa gggaaccgta tatttatctt
    10561 tatctgaaag catgactaat ggaaatagat attgattcca acttcccata aagataagta
    10621 aaccactagt aataatcgta ggtgataaaa tcggtacagc gattttcgta aaaatcaccc
    10681 actcattggc accatcgatt ctggcagcct ccatcagtgc tgtaggaaat tgactgctat
    10741 attgtcgcat cataaacact gaagttgtac tggcaatcga agggatgatc acacctgcat
    10801 aagtattcga taatcctaaa cgactcataa tgtaaaataa aggaatcaat gtggtaaaaa
    10861 aaggcaccat cctagatagc ataactgtat tgaacaacag tttattcccc ataaaacgat
    10921 actttgctaa tgcataaccc gccattgtag aactggctgt agctaaaact gtactgatta
    10981 cagcaataac aatcgaattc caaataatca agggataatt atatctcgcc atgatggctt
    11041 gccagttttt taatagcata tccccaaact tgaaattgat tccgccactt aaaatttctt
    11101 gattactttg cgttgaagct acaatcatat ataaatatgg catcaaaaat attaaggcac
    11161 caaacacaat aatgacgtac gtaaaaattc tgctttgtat cttttgttta gtcattttta
    11221 tcacctccga atttaaattg aatagccgat aatataacag atacaaacac catcgtccag
    11281 gcaatagcag aggcttttcc aaaatcaacc atttcaaaac ttgttttata taaattgact
    11341 cctacgacca aagctgaatt ttctggtcca ttggaagtcc caaagaatat atttgggata
    11401 ttgaaattac ccaagccatt gatagtggct aaaactgtgg ccattaaaat gattggcttt
    11461 agcatgggga tcgttaataa aaagaattgc cgaaatttcg atgctccatc tatttcagca
    11521 ctttcgtata tctctttaga aatcccttga agaccagcta aaaataaaat agtgttatat
    11581 cctacactaa gccaaatcat cgtgatgatc attgatattc gtatccaaaa aggatttgtt
    11641 aaccactgta caccgttaat tcctagcgca ttcaaaaatc cattgaataa cccagtcgga
    11701 ttgaaaaaag tcatgaatac ggaagctaca gccaccgttg atgtaatggt tggaatataa
    11761 tagacagttc taaaaaatcc ttttcctttt aactgtgctg aattcaataa aacagcaaag
    11821 taaatagcta atagcatcgt tattggaatt gatcctgcca tgataatcaa aacattcgtg
    11881 taagatttaa taaacaatgg atctcggaag atagctagat aattgtcaaa tccaacaaat
    11941 tcaaatgagt agccaatttt cataaaacta aaagcaaacg agataatgat gggaatcaaa
    12001 atcgtcacaa taaagaaaat aacaaaaggt gtgaccaatc cataagcact ttttttatga
    12061 gacgtatctg ttgctttttt cttgctgttc tttattgtct tgcttggtaa cgaagattgc
    12121 atcatcgtta agtcctccta atatttttta atcaccagtt aattttacgc cgtacttatg
    12181 agcaaaattt tctgcttctt ttagatatga attttcaaaa tcacctgttt gccaatagtt
    12241 atatgcagcg gtagtcaaat aagacgtagc atctgaataa gtggctggta aaacagtatt
    12301 tgatgtattt tcattgattt tattcaatag ttcatagtat ttttgtcctc cttagtagtc
    12361 aaactttttc tcagctgctt ctgtttgata ggccaatgag ttagatgtag ccacgcccag
    12421 ctccagtgcg ttggctaagc attcttcatc tgtcagcata aatgtaagaa attgttgtgc
    12481 cacttgtgga tttttggcat ctttagggac ataaaaactt gatcctcctg atactgcacc
    12541 ttttcctgga ttttcttttg aaaaaggaat cagtggagcc atgcgccatt tgtcagatgc
    12601 atcaggaaaa ttcaaagaca tattcgtagc ataccagccg ccagcaacaa acattgcaga
    12661 ttcttgaaat gctttctcag catcttgtgc accgtagaaa tgtacaatat ctgagtcgat
    12721 catttctttg attaactttg cagcatgttt cgcttcttta gtattcagac ggatatttcc
    12781 ttcttcatca agcaaagatt gattctgctg ggctagcatg ttgataaaca attcttggtc
    12841 cccagattct ggcaaggcaa ttccaaaact accagttttt tcttttaatg cttttgccac
    12901 ctggatatat tcttcccaag aactgatttc ttcaaaatct acacctattt cagcaaattt
    12961 ttttggttga taataacaaa gtgctggggt aaaatcacta gcccaaggaa taatcgtttc
    13021 attactcgtt tgttgacgca aaatattcaa tttttgatca taaaactgat cgccatattt
    13081 gtcataaaat ccatattcat cagcactata aaaagcatct tgaaattttt ccagcacacc
    13141 tgctgctgaa gtgtctgcca tggagataat atccggcata tcttcgtgtg ttaccaattt
    13201 tggggtgatc atttgtactg ttgctgttcc cgaagctaca ttcacaattt ttaagtcaat
    13261 atttgctcca gttttgcggt tatagatttt tgcagcattt tcaacatagt ttgtcagttt
    13321 gccgtttgcg acatatactt ctaatgtagt ggcttttttt atcattggat ctgcgatgtt
    13381 tgctgttact tcttcgttag tttttccaca tgcacttaag ctgaataata gggtaatgtt
    13441 tagcaaagct atcaccaatt tgtatctttt tttcatgtct atcccttctt tcttttcaag
    13501 ttcttatata aaacgctttc acttatgtat attaacggaa ttagtaacac aaatatttat
    13561 ataaaaaaat attttacttg tcgttttggc ttttcatttt tgttataata gtcattaatt
    13621 ataaatagaa agctttttat ataaaaagag aaaggaaagt ggttcttttg ttaacaaaca
    13681 accaatcctc caatcttgtc aaaaaaacac taacctattc catagatacc catcttcatt
    13741 tatctgctgc tccctatttt cgactactaa taaatgaaag cgctaattta tatgctgaat
    13801 ttgcacataa atctatagaa ctagataatc cttccctttt attcattgat aaacagcaag
    13861 aagtccaaat cttttctact caaaaggaaa cacaaacttt tatcattatc cagttttatt
    13921 ttgatgagca attattagca catattacgg gacaatcttt ttctatagat caagaaataa
    13981 catctatcat tacttttctg aaaaatgaac agttcaaaat tgattatcta aaacatagta
    14041 agccagctat ttcctatgat caggaaatca attctttttt catactatgc caacagatga
    14101 gcattcattt gctttcctta ttagttactt attgtctgca aaaaattcaa ttgtcttcct
    14161 taccaaggca ttatcaacag gaacttgctt tattgacaat accttcaaat gagaaagtca
    14221 ttcctaaaaa aaagcatatt ctt
//
LOCUS       DARDHA010000062        14043 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000062 DARDHA010000000
VERSION     DARDHA010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14043
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.062"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            55..723
                     /locus_tag="SDA10_001494"
     CDS             55..723
                     /locus_tag="SDA10_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4240410.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            726..1769
                     /locus_tag="SDA10_001495"
     CDS             726..1769
                     /locus_tag="SDA10_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG4240411.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(2006..2887)
                     /locus_tag="SDA10_001496"
     CDS             complement(2006..2887)
                     /locus_tag="SDA10_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG4240412.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            3046..3774
                     /locus_tag="SDA10_001497"
     CDS             3046..3774
                     /locus_tag="SDA10_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEG4240413.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            3930..4247
                     /locus_tag="SDA10_001498"
     CDS             3930..4247
                     /locus_tag="SDA10_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG4240414.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            4240..4848
                     /locus_tag="SDA10_001499"
     CDS             4240..4848
                     /locus_tag="SDA10_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEG4240415.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            4903..5694
                     /locus_tag="SDA10_001500"
     CDS             4903..5694
                     /locus_tag="SDA10_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEG4240416.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            5832..6344
                     /locus_tag="SDA10_001501"
     CDS             5832..6344
                     /locus_tag="SDA10_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEG4240417.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            6370..7242
                     /locus_tag="SDA10_001502"
     CDS             6370..7242
                     /locus_tag="SDA10_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEG4240418.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            7418..7780
                     /locus_tag="SDA10_001503"
     CDS             7418..7780
                     /locus_tag="SDA10_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG4240419.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            7789..8541
                     /locus_tag="SDA10_001504"
     CDS             7789..8541
                     /locus_tag="SDA10_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4240420.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            8555..10357
                     /locus_tag="SDA10_001505"
     CDS             8555..10357
                     /locus_tag="SDA10_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4240421.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            10634..11674
                     /locus_tag="SDA10_001506"
     CDS             10634..11674
                     /locus_tag="SDA10_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG4240422.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(11726..13870)
                     /locus_tag="SDA10_001507"
     CDS             complement(11726..13870)
                     /locus_tag="SDA10_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEG4240423.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
BASE COUNT         4418 a         2372 c         2968 g         4285 t
ORIGIN      
        1 aagatgaact acaagaaaaa atctagaaat ttttcaaaaa acagagggag atcaatgaat
       61 cgtattttag tagtagaaga tgatccgcaa ttacagttgc tttaccgttc tgtgttggaa
      121 cgagcgggct tttcagtcat caccgcattg aatggagagg atgcgctgaa aaaactagaa
      181 gcgacacgta tagaattaat cattacagat atcatgatgc cgaaaatgga tggctatgac
      241 ttgctagact cattacgtag caccagattt gacacacctg ttctaatgat tacggcaaag
      301 gccgattttg aggataagaa acgcgggttc aaattaggag cagatgatta tatgacaaaa
      361 cccatcgatg tcaatgagat gatcctacgt gtggaagcat tgcttcgacg ttcaaagatc
      421 aattacagca gtagcttgat aatcggacaa acaaaattag agctagatgc ttatacagtg
      481 ttacaagatc agcattcgtt gattctgcct caaaaagaat ttctgcttct gtataaatta
      541 ctgtcttatc ccaacaagat cttcactcgc caacagttga tggatgatat ttggggatta
      601 gatacgaata cagaagaacg aacgatcgat gtacatatca aacgtttaag gacccgtttt
      661 gaaaagacgg aagattttca aatcatcaca gtacggggac taggctataa ggcggttatt
      721 taaagatgaa aaaaaagcta ttttcacaat tatggatcta ttttgctagt attgtcttca
      781 tttcgatctt agtgacactg ctgtgttttc tcggttttat tttcttattg tctcgacatt
      841 ctatctcgcc tgctgaatcg gctggaaaac caacgttata cccgttattc gtctttgcgg
      901 ggttcagtat ggtcgtgggt acaggaatat cgatttttgt tgggcggcgt atcctgcatc
      961 caatcagtgc gctagggaca aatatgagtc tggtagcaac gggagatttt tcgattcgaa
     1021 tggatgaaca gcagaaagta gcagaagtac agcaactgta taaagatttc aacgttatgg
     1081 tccaagaact caatagtatc gagacactga ggaatgactt tgtgtccagt gtatcccatg
     1141 aattcaaaac accactagca acaatccaag gatatgttca attgttacaa gccccaaatc
     1201 tctctgacga agaacgccaa atatttttat atcggattat cgaaagtatc acgcagcttt
     1261 cacaattaac agagaacatc ttaaaattga acaaactgga aaaccagcgt atccaactgg
     1321 aaaagaaaga gtatcgatta gatgaacaaa tcagagaagt aatcgttttt ttgcagccta
     1381 aatgggagaa agaacagcta gaattggata tcgaacttgc tgcagtaaat tacacaggca
     1441 atgaagagtt tttgtaccaa gtgtggctga atatcatgga taacgccata aagtacaatc
     1501 aaatcaatgg acagattcat atcaaactat ttgagacagc cacagagatt ggactggaag
     1561 tgaccgattc aggtgtagga atgaatgaag agaccagaga tcgaatgttt gaaaaatttt
     1621 atcaaggaga tacaagtcgt caaatttctg ggaatgggct aggactttca ctggttaaaa
     1681 aaattctaga actgcatgat ggacgtattg attatagcag tatcgaagga gtaggaacga
     1741 cagcaatgat acggttaagt aaacaatagg aaaaagaata aaacccctaa aaattctatt
     1801 gaaggttttt aggggttttt agtcgttttt ttgtgattag ggaaatgttc ttacggttct
     1861 tttggcataa aaatcgctgc tttcttattg aactacgaga aatgttagag gtgtcattga
     1921 aacaaggaca tgatgcaaga aagagagcga aagtcatgcg tcgctctctt tttttgggtg
     1981 gatagatgga cagaaaatgt agggcttagt tttcttcttg atttaaatac tcccaaaatg
     2041 tatctgaagc caaagaatgc tcaccgactt tacgagtaaa tgcaagataa ctctctgttg
     2101 ttggagaaat agggagaatc acagtatttt cttttaatag attcttgact gttttttcca
     2161 taacaatagc aatccccatg tcatttgcaa tcatattgat gatcaagtcg attcttgctc
     2221 ctttataaga gatttttggt tcaaatccag cttcgcgaca cagatctagt acaggttgat
     2281 acaagttcgt agtggggcca aggagcaaaa agttctcctt tttcagttcg cttagatcca
     2341 atttcttttt actagctaaa ggatgatttt taggtagaac tgctacaaaa tcgtctgttt
     2401 cagtaggcaa ccactcaatc gaacttggtg gggcctggaa atttctagca aagtggatcg
     2461 tgtcactatg ttcattggcg tcattgtaag ttaattcgac actttccact tctttcaact
     2521 ggactgtaaa ttcaggatgg cgtttcaaaa actcggtgat tttggaaaaa cctttataat
     2581 tgatcattgt cggtatcgtt aagatattta atgaaagatt gttgtctttc gtataggttt
     2641 caagcacttc ttgcatagca tggtattgtt ctacgacatt ttttacatat gggagcaata
     2701 agcttccagc ttcggtcaat tcgatttttc tatgggcgcg ttcgaataat ttgacgtcta
     2761 attctgtttc taacgaaaga atctgtttag aaatatttcc ttgggtggtg aattgtcgat
     2821 cagcagtttt ggtgaaattc attgtttcgg ctaaatcaag aaaaacttcg aatttatgga
     2881 tatccatcat attcctccta cttattccaa taaagaatac tttaacacaa aaaatgaatt
     2941 ggtgtaatct tctctgtctg ttacaatctt tcttgcagcg aaagcaataa ttacagaaat
     3001 ttttctgatt tttttcgctt cgtagaaaaa gagaggagtt ttttgatgcc aactggagtt
     3061 ttgatcaatg taggttccgt tttgcttggc ggactaattg gcggattagt aggaaataaa
     3121 ttaagtgaac attttaaagc acagctgacg atggtttttg gtgtgtgttc gatggggatg
     3181 ggaatctatt ccattgcgcc gatgaaaaat atgccggctg ttatttttgc tttagtgata
     3241 ggaacagcga ttggtctgat tgttcattta ggtaatggga taaataaggg ggctgcgctg
     3301 atgcaagtac ctatttctaa aatcttccct agtgaaaagc ttggtatgac ccacgatgaa
     3361 tttatcagca cattagtgac ggtcattgtt ctattttgtg caagtggtac ggggatctat
     3421 ggaagtttag attctgggat gacaggagac agtacgattt tgatttctaa atctgtcctt
     3481 gattttttca cagcagctat ttttgcgtgt aatttagggt atgtcgtttc tgtgatagca
     3541 attccccaat tcattatttt ttatattttg ttcttattgg caaaatttat ttatccattg
     3601 actacaccag acatgatctt agattttaaa gcttgcggcg gattcttgat ggtcgctacc
     3661 ggttttagaa tgatcaacgt gaaaatgttc ccagtagctg acatgatacc tgcaatgatc
     3721 gttatcatgc cactgagctg gatgtggaca aattggatta tgccactgtt ataataaaaa
     3781 tcaaagagaa gccatttttt gctgactaaa aaggatcaaa tcttttagtc agtttttttg
     3841 tatttgacaa actgattttg aaactatatt atatgttgta taatatagat tgaaataaaa
     3901 tattacactt gagaatagaa ggtgacgtaa tgcacatcca gattccaact gttcttcttg
     3961 atggtactgt gctagcagca ttaagcaagg aagatatgta tgggtatgcc ttaacaaaaa
     4021 tcgtacaaga aagtctctct gtcagtgaat ctacactata tccggttttg agaagattga
     4081 aaaaagaagg atatcttgag acctatgatg aaccttttga aggcagaaac cgtcgatatt
     4141 actcgttgac accaatggga agtcgtcatt tagaagaaat tattgatgaa tggattgttt
     4201 ttcgagattc tttagaccgg ttattggagg agagcacaaa tgaatgaata tttgaatgaa
     4261 gttgtggatc atcttcaaga aattcctgaa gacgatcgtt ttgatttgat ccagtattat
     4321 gaggaatatt ttttagattc tggaaaaaca ttagatcaga tcattgatga atatggtaca
     4381 ccaaagcagt ttgccttaaa attaaaaatc agttactttt cagaagaaga acaagcagaa
     4441 aacgagttag tgaaaaatcc aaaaagacaa attcgcctga tttggttaat cattatcggt
     4501 ttatttgctt ctcctgtcct cattccacta gcactggggt ttatattggt tattggggga
     4561 ctattggttg ctttactagc gctcattttt agtatctatg ttttatgtat cagtatctta
     4621 ggtgttggcc tattcatgat catctcagga atcagtgtgt ttggacaatc gattctgagt
     4681 ggattattct ttatcggtgt cggtctgttc gccacaggaa gcgcagtatt tatttcgcca
     4741 ctgttgctga aaggaacaaa agggcttttt tatgtcatga tgaactttgt taaatggatg
     4801 ggacgacgtt ttattacaaa aagagatctg cattcagcgg gcgtttgaag gaggacacat
     4861 aaattgagat tgaaacattt tttgattacc ggtattggtc tgatgcttac tggtggcctc
     4921 ctagcaggtg caacttatac attaggcgct caaaagagtc tgacttggga taatggtcct
     4981 cggttgatcg atatggttac tgagacaaaa aaaatcaagg atgcaaacaa aatcgtcatc
     5041 catgcagaaa atcaaacagt ttcggtcata cgcggattgg attttgagat aaaaacgact
     5101 tatgataaga cagaaaagcc agaaataaag gagaaaaatg gcgtgttgac tgtgaatgca
     5161 gtcaaaaaat caacaggtgc aatcattagt ttagaagaca caagtgcaca aatcactatt
     5221 gttgtccctg cagatcatga attatctgaa ttgacaatcg aagggaataa tagtgcaatt
     5281 gatatcaacg aagtatcaat caaaaaagtg aatactaaga cacatgatgg ctgggtatcg
     5341 ttcaatgaat ttacaggagg aacaataact gcgaaagatg aattaggctc acttcgatta
     5401 gatgaggcca atgtggataa cttgatcgtc acaggtgaaa acagcaactt ccatctagaa
     5461 gacagtttga tcagcaaaga aggaaaagtc atcctaaacg gtggcaattt atctttgcat
     5521 gagatggaaa acagtggcta tactatccaa acgtctggtg aatcttatat tgaaaaagac
     5581 taccagccaa taacaaatac tttgttagaa caaggacaga aaaagctaaa gatcactgct
     5641 aaagacacat cgatcaatat tacgacggat caagaggaca atgaaacaga ttgatcctgt
     5701 actcaactgg atattttaag aaaaaacagc aaagatcctt ccaatctttg ctgtttttca
     5761 tcgcttaacg cacataacta tagtaaaata agaaagaaaa tatctaaaaa gaaaacaggg
     5821 tgaaagagta tatgaaggta gaagaaggtc aaagagaaaa gttaaaaaca gaaatggaaa
     5881 gattacatac ttatatcact caactaagcc aaaccttcta tgatccagac aaagaaaaag
     5941 tgatggttaa ttacccgaac aattcagaag gtcgccaatt agagcaagtg taccatgaag
     6001 ttttcaaaca tttattgacg gtgaagaaag agctggatta ttattcttta ccgattatcg
     6061 atacaggaat attgaagtat gatgggaaga aggaacgatt tatctttaaa tctgttcggg
     6121 aagatttgcc tctttcagca gggatggatc tagaggtctt ggtagaagac tattttacag
     6181 aagaaaagca ctgggttcga acaaaattgg attatttacc gcaggcagcc ggagggacac
     6241 aggcaagggg ttggtacatc actgaagaca aagagttaga actagaaggt gtaatggcgc
     6301 gtattcgaaa aaaaattaaa aaagtaacat tagatattta ttgaaggata aaggagcgca
     6361 ggaagtagca tgagagaaaa aagagcattt tatatcaatg gttatgcagg gattgtcgga
     6421 ttggtcgtat tagctttagt tggattgttt ttcttctatt taggtatgtg gcaagcgaaa
     6481 gtttgggcac tttttttaag tatttttctt tggttgatca cgttattgtt gttaagttcg
     6541 gcaactgtag tcagtcctaa tcaagcaaaa gtaattttgt tttttggaca atatttaggt
     6601 acgattcgag aaaatggctt tttccttacg atacctttgg cacaaaaaat gactgtttcg
     6661 ttgaaagttc ggaatttcaa tagttctgtt ttaaaagtca atgatttgga tggaaacccc
     6721 atcgaaatct cagcagtagt agtctttaaa gtcgttgata cagcaaaggc attattcgat
     6781 gtagcgtatt atcaagattt tgtagaaatc caaagtgaga cagccattcg gcatatcgct
     6841 agccaatacc cgtatgatac attcaatgag aatgatttga cgcttcgagg aaacacgacg
     6901 gcagtgtctg acgagttgca aaaagagctg caagagcgtt tagcagtagc aggtgtcgaa
     6961 gtaatcgaaa cacgcttgaa ccatttagcc tatgcaacag aaatcgctag tgccatgctc
     7021 caacgtcaac aagcaaaagc cattttgtct gctcgacaaa ccattgttga aggagctgtc
     7081 acaatcactc aaatggcctt agagcaaatc gaagatggac aagaaatcaa tttcaccgat
     7141 gatcggaaag tccaattgat caataattta cttgtctcaa tcattacaga caaaggaacg
     7201 caacctgtta tcaatacagg cgacatgaaa gaccgtaagt gacatacttt ttgtaagata
     7261 ggaagaagga gtttaggacg gattattgtc ttgaactctt tttttagttg ttttaaagaa
     7321 aatgacaaaa ttgaaagaag aatgatagtt gattcgaagg cagaattgtt cgcttttttc
     7381 tataatcagt atataaagta agagagaagg ggaaaaagtg aaaaaacttc tgatcggtct
     7441 acttgttttc atcgttgtgg tacttgcagg ggtgaaaatc gcagattacg tagttatggg
     7501 tggagaagaa tactatgtac aaattacgac agatggtaca aaaacagttt caaaatcaga
     7561 ctctggacaa gaaacagtca actatagata tatcctgcct ggctatacga aagagggtaa
     7621 ggaaaaagaa ctggacttca agggacaatt gactcgaccg cttcgtaaag gagcatattt
     7681 aaaaatcacc tggaacaaaa ataaaggtgt cacttcgtat gaagaagtcg aaaaaaaaga
     7741 cgtgccgaaa acagcactgg aaaaactaat taaagggtag ggaaagaaat gaacaaaata
     7801 gtcgaagtac agaatgtaac aaaagtatac ggaaaaaaca atgagaaaaa gacgcaagca
     7861 ttaagcggca tcagcttcga tgtagaaaaa ggcgaattta ttgggatcat gggcgcttct
     7921 ggttctggta agtcgacact tttgaatatt ttatccacat tagataaacc aacagatggg
     7981 catatccgaa tcaatcaaaa agatgtgacc acattaaaag gaaatcaact agcagatttt
     8041 cgtgcgaatg agattggctt tattttccaa gatttcaatt tattagaaag cttgacagca
     8101 caagaaaata tcgctgttcc tctttcttta caaggcgtca gaccaaaaga aatcaagcaa
     8161 cgtgtccaaa aaattgcaga acgtttatcc atctctcata ttttggaaag ctatccttct
     8221 gagatttccg gtggacaaaa acaacgggtc gccgccgcac gtgcattgat cacacaacca
     8281 acgattctat taggggatga gcctacaggt gcgctggatt ccaaaagtgc ccgggatctt
     8341 ttagatacga tggacgaatt gaatacaaaa gatcatgtat cgattcttct tgtgacgcat
     8401 gatccgtttt ctgcaagtta ttgccaacgt attttattca tcaaggatgg cggtatccat
     8461 caagaagtga aacgtggaga tcaaagccgt gattccttct atcgagaaat actatctatt
     8521 cttggaaact tggaacagta gggggagatt gactatgctt tggaaattat cacttactgg
     8581 gatcaagagc cgtttaagag actacttggt gttattctca ggattagtta tggcatctgc
     8641 tattttttac atgtttgaat caatggcaag caacgaagct tttttgaaaa gtaatacgat
     8701 cgttgcggct acagttatca ttttccgatt cggttctgtt ttactaggga tcatcacatt
     8761 cgtttatatt ttatatgcaa attccttttt gatgacgatg cggcaaaaag actatgcaat
     8821 gtttatgatg ctgggagcaa aaggcagaaa aatcgcacag atgatttttt ctgaaacatt
     8881 cttagtaggg atcgcagcta ctttagtggg ttcagctgtc ggtgtaggat tgacaagtat
     8941 cgtccaccga ttattagtgg atcagttaga tctcaatatt tcgcatttta caccttttag
     9001 tattcacggg ttgttgatca ctgttttgtt ttttgctgtt ttgtttttat tagctgctgt
     9061 tgtcaatgct ttttcgattg tcaaaaaacc gattttagca ttgattcgtg cggatcagac
     9121 accaacaaga atcaagcagc ataaattctt gtttttcctt gaagtagtac tgggaattgc
     9181 ttttctaggc tgcggttact atatgatgaa acacgtgggg acctatcaag tctttggtat
     9241 aggggtagcc ttagttacga tcatattagg aacttatttc attttccatt ctgtgatcat
     9301 tttcttttta tcattactaa agaaatcaga acagatttca ttgaaaaaat tgaacaattt
     9361 cacattgtca cagttaaact ttcgtattcg tgaatataca caaatgctct caatggtcgc
     9421 tatgctgttt gccttggcac tgggcgcttt gacggtagga ttaggtttta aaaatgaaat
     9481 tgcagacttg acagaaaaca attctggata tgatctagtg atgaataatc ctcgggcaga
     9541 agatcagcaa aaaatcaaag aactttcacc aacactttct gcttcttata ctcaaaaaga
     9601 agatgcggga acgatttact ataatgcaag tgaatttgcg gctcaaccat tgatcatgat
     9661 aaaacatgaa gatggaacac cgccgaagat cacgaaagaa aaagttcctg ctaaaaaaat
     9721 gaatgaactg actgtacaag aggaacttcg aagctatgaa ttgccggaac agcaagaaaa
     9781 agaaatcaag gtagtcagtc aatcagaatt tgatcaactg aacttgccac aatccacttt
     9841 acagctagtt caggtaaaag acttccaagc aaacctgaaa ccaatcgaaa cacttgttgc
     9901 tcaaaataaa atgaacaatc catctttgca aaaagtagag tacagcaacc agaaatatga
     9961 aatgtatgaa gtgtataatg gtctattctc tggttttgaa tttatgggat tcttcctagg
    10021 tttagctttc ttgaccatgc ttgctagctg tttgatgttc aagatcttgt caggaagcaa
    10081 gagtgacatt gctcgttatg aaatgcttga aaagatagga acacgacgcg ggttgcttaa
    10141 acaatcgatc cgtagagaag taggcgtgtt attcttagca ccaggaatct taggtgtagt
    10201 ccatgtctta ttcggattac aaatgtttgg tctattcatg caaaatccat acaaagatat
    10261 ttgggttccg tttacgatct tcttcgtatt gtatttcatc tactatgtac tgacgacatg
    10321 gttatatact ggaatcgtat tgaaaaagag attgtgaaaa agctgtcctg catcaagtaa
    10381 taagaacagc caagcaaaaa tagctcctca tatttttcgt ttggttgggc ttattaccgc
    10441 agatgcaagc ttttgaacac cgtttgttcg gaaaaagagg ttgtgacaat atttttgtca
    10501 caaccttttt ttcacggtct tttattagaa tatagtaaat tttttctcgt tactcacgga
    10561 ataagctgtt aagaaaacgg tttccatgta tactggaatc agtgcaaatg aaagaaaaga
    10621 gggtgcaagg aacatggaaa tcaatgaaca aaattttggc gaaaactatc acttgattac
    10681 gatcacgaat caaaatggga catctttgtc tgtcagtgat cttggggcac gggttgttca
    10741 ttttcaaaca ataatcgata atgaaaaacg agagttgatc ttaggttttg atgaagcaaa
    10801 agaatatcta gaaaaagacc cttatatcgg tgcttcaatc ggacgtatgg ctggacggat
    10861 tacgaatggc acattcaaat tagatgatga aacgttcgaa gtaaaaacag acccggacac
    10921 tggtcattgt ttacatggtg gtgcacctag ctttgaagcc aagcagtggc attatacagt
    10981 catcaatggt gaaaacgaag catcggtcat tttctatcag actagcccag atatgggaaa
    11041 cggatttcct ggaactttag atgtagaagt acggtatacg ctgacgaacg atgatacatg
    11101 gcgagtgacg gtccaaggga tcagtgaccg caaaacagta ttcaatccta caaatcatgt
    11161 ctatttcaat ttaaccggtg atccagcaca gtcaatcgac gaacatatac tttggttgaa
    11221 tagccgatta tttgctacgc tcaataaaga tacaacgcct acaggagaag tagtttcagt
    11281 aagtggcaca gcatttgact tccaacgtcc taaaaaatta ggtgaggtct ttgccagtga
    11341 ttttcgccaa tcaatgatag tgggcggcat cgatcatcca tttttgttga aaaaacaatc
    11401 tggtttacta aatgctgcgg aattgacaag cccagatgaa aaagtatcga tccaagtaaa
    11461 aacagatgct tcaggaatcg tgatttttac agcaaatttt ggagaagatg gaccggaaat
    11521 gcgcgggaat aaattagtaa atcatggcgg tatcacattt gagactcaag aactgccagg
    11581 agcggagcga cttgaaagtt ttggagatgt cactctggaa cctaaccaag tacgcgagtt
    11641 tgtaacggaa tacaaaataa atataaagaa atagaataaa ttaaaaaggg agtgtgacag
    11701 tgtcacatcc cctttttaat ttattctatt tctttaaagg cagatcaagc cagaatgatt
    11761 tgtctaaact taacaaggtt agtacaggaa cagttgcagc tttaactgta cgagtagttg
    11821 tttctggtat ctgtcttgcc gtaaaattgt tccacaaatc ccagattgaa gcgacacgga
    11881 agatggaaga agcgttgttg acgcgtttta tcgttgaaaa ttctaatcct gtgattggca
    11941 cgccatctgt tgtccatttg attggcacat cgaatcgctt agacatattg aggatttcag
    12001 cgtttgcttc ttcagttgtt gctgcttctt ttttcggttc atcatatttt gtcaggtcgt
    12061 acgcttcgat gatcgcgttc aatttttgtg aatattggcg atctgtagca taacgtcctt
    12121 ccaaatattt ggttgcttct ttgtagtctt ttgtttccga cttccaagtt ggtttgtaga
    12181 aatcaggatt gccagtcagt ccatttttga tcaagtcggc atagtcttcc aaactttcct
    12241 tgtaagaagg atatttacgg aattgtgcgc ggatttggta gctttgtccg gaactatctt
    12301 gttcaagtgt attgaaggtc acgctgcttc ctttatatga tcctttgata ccaaaaaggt
    12361 tatgatttgg ttcgcttgat aataagctgt ttccagatcc actttcaagg atagcttgag
    12421 ccatcatgac tgatgcgtag attccttctt tttgcccgat ttcctgtgca tcatctgcaa
    12481 tctttttcaa gaactgatca gttgtttggt tttgactgaa atggaacgag ccatctaatc
    12541 catctaaaat agatgagccg cctgtcagcg aacctgaagc agcaggtaaa cctgctaagt
    12601 ttactcggac agcaaaatca ggcgtgaaat ctgaaataga agttactcga actttatctc
    12661 ctggttgagg cgcatggata aattgtccgt tcccaatata gatacctaca tggtgagtaa
    12721 caccggaaga aggtccccag aagacgagat cgccaggttg ggcctgactt actggaatct
    12781 gcgtacctgc atattgctgt tcgcctgtcc agccaccgat ataatgaccg gttgctttca
    12841 tatatacgta ataagtcaaa ccagaacagt caaaagtatt tggacctttt gctccccata
    12901 catatggttt tccaagatgc ttgaatgctt ctcgaacgat tgcttgttgg acattcgttg
    12961 tatttgtagg caatacagca tcaattggtg attttacaac aaaatcagaa gaagcagaag
    13021 atggattctc tataccgctt tgatttgctg tatttggacg agatgaagca ttgttcgttc
    13081 cgccattcga tgaaccattg tttggatggt ttggttctct ctgagtattt ccattgttat
    13141 tcgatccaga tgggtttgtt tgattcgaag aattcccgct gttcgaatta ttgttattat
    13201 tgtttgaatt atttgaacca ttattattgt tgttattacc cacatctggt ttgtttggcg
    13261 tttccggatc tggtgtacta gggacttctt cactaggatc agttggtttc tctgttgaag
    13321 tatccggtcc agctttaaat tctgcagtaa tagataaatt gttcgtacct actgtaaagg
    13381 tctttgcatc ataatttact tctttggcac cattaacagc gactgaagca atttgttcac
    13441 cttcagctgg cgtaatagtg taagttacct tcgtttccgc taataaacct tctaccgttc
    13501 cactttccgt aatatccaaa ggttgctgtt caggggaatc cgctgtcgaa gcagtcagtt
    13561 ggatcttacc aatcaaagaa ggagcaattg taagggaata tgtctcggct ggcttttcat
    13621 tttctgatgg atcagccgga tcaatttcag ttggctcggt cgatcctgtt gttgagttag
    13681 ttgttccagt tgggtcttct gtaaaagaat ccgttgtttc actagtagct gagctggatg
    13741 tagtcgtatc tgttgtgact gtttgatcag ctatttgatt atctacggca aatacggtat
    13801 tggtcgacaa aacagacgga gcaatcagcc aagtagcagc tagcaaacgt atcgtttttt
    13861 tatttttcat aataaactag tccttttcct ttcattgatt ttctgtctta ttcagcattc
    13921 attagacatt ttttggaaca gataatcgtg tcgaaaagct ttttcttttc ataaatctgt
    13981 ctgtgtattc taaataaaac aatttgtaaa taagcaacga gcattcattc gtttttcttt
    14041 gac
//
LOCUS       DARDHA010000063        13976 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000063 DARDHA010000000
VERSION     DARDHA010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.063"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            524..856
                     /locus_tag="SDA10_001508"
     CDS             524..856
                     /locus_tag="SDA10_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4240424.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            766..960
                     /locus_tag="SDA10_001509"
     CDS             766..960
                     /locus_tag="SDA10_001509"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG4240425.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            1152..1307
                     /locus_tag="SDA10_001510"
     CDS             1152..1307
                     /locus_tag="SDA10_001510"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEG4240426.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            1583..2923
                     /locus_tag="SDA10_001511"
                     /pseudo
     CDS             1583..2923
                     /locus_tag="SDA10_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3164..4780)
                     /locus_tag="SDA10_001512"
     CDS             complement(3164..4780)
                     /locus_tag="SDA10_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEG4240427.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            4898..5116
                     /locus_tag="SDA10_001513"
     CDS             4898..5116
                     /locus_tag="SDA10_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4240428.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5311..5772
                     /locus_tag="SDA10_001514"
     CDS             5311..5772
                     /locus_tag="SDA10_001514"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG4240429.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5965..6204)
                     /locus_tag="SDA10_001515"
     CDS             complement(5965..6204)
                     /locus_tag="SDA10_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4240430.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(6228..7751)
                     /locus_tag="SDA10_001516"
     CDS             complement(6228..7751)
                     /locus_tag="SDA10_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEG4240431.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(7769..7891)
                     /locus_tag="SDA10_001517"
     CDS             complement(7769..7891)
                     /locus_tag="SDA10_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEG4240432.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(7921..8904)
                     /locus_tag="SDA10_001518"
     CDS             complement(7921..8904)
                     /locus_tag="SDA10_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG4240433.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(8928..9077)
                     /gene="rpmG"
                     /locus_tag="SDA10_001519"
     CDS             complement(8928..9077)
                     /gene="rpmG"
                     /locus_tag="SDA10_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG4240434.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(9098..9475)
                     /locus_tag="SDA10_001520"
     CDS             complement(9098..9475)
                     /locus_tag="SDA10_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240435.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(9507..9686)
                     /gene="rpmF"
                     /locus_tag="SDA10_001521"
     CDS             complement(9507..9686)
                     /gene="rpmF"
                     /locus_tag="SDA10_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEG4240436.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(9701..9970)
                     /gene="rpsN"
                     /locus_tag="SDA10_001522"
     CDS             complement(9701..9970)
                     /gene="rpsN"
                     /locus_tag="SDA10_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEG4240437.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(10493..12259)
                     /locus_tag="SDA10_001523"
     CDS             complement(10493..12259)
                     /locus_tag="SDA10_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4240438.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(12219..13972)
                     /locus_tag="SDA10_001524"
                     /pseudo
     CDS             complement(12219..13972)
                     /locus_tag="SDA10_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT         4449 a         2698 c         2142 g         4687 t
ORIGIN      
        1 taaagaataa ctcaactaag ggagtctgtg acataagtaa aaagatagtt tcaaatttcg
       61 aacaataaat ttgaaactat cttctttata attataaagt atcgatttta cagcgttcta
      121 taatgattca tagaatctaa aacgaatgat tttaattatc aacgtttttt aattcgtttt
      181 tagcttaaaa ataacttttg tcacagtctc actcctaaaa ttactttatt tacgtatgaa
      241 gtgggcagga ggtcgaatcc agaactgggc tatggtactt caacgaggac ttctcctcta
      301 gaattgaaaa gcatgcgatt taccttccgt aacgacggta gatttattca actatgtgtc
      361 caatcaaaat ttgtctgatt tcaaaaatag tcaagagaat tttggttgtt aaaaactctt
      421 atctatcaac ttccacagtt tagttgacaa accctgatac taagttgggc tatgatacac
      481 ttttcttgac tatcagttac gttaccggaa ggaatgaact ctattgagaa aaacatatga
      541 tcgtaaattt aaactaaaga tttcacagga tatccttgag aaaaaagtaa ctaccaagaa
      601 aattgctgaa gaatacaata tctctcgtcc aacaatttcc cgatgggttt cagaatatcg
      661 tcgatacggg aaaaatgctt ttgctggaca aggaaaaaga ttacctgata aagcagattt
      721 ctacattctt gaacaggaaa ataaacgact aaaagaggaa gacgagtgtt ctacgaacac
      781 aaagatcgat acggatctcc agggatcact caaaaacttt acgatgaagg aatcgaaatc
      841 aataaacgtg ttgtagctgt tcttatgcgt gaaatgaatt tatgcacaag aggatttcat
      901 cgacgcaaat cttctcatgg tagagaaaaa acaatcaaag aaatggtaaa aaaatgttga
      961 atcgataatt tgaacagaac caaattgatg ctgtttgggt gatggatatt acttacattc
     1021 catgtaatga cggtcgcctc tatttgtcca cctatattga cttagcaact cgtattctac
     1081 gttactatat ggtagattca cacatgaaaa aagagattgt cgtccgtcca ttacaaattt
     1141 attaaggaga attgcctacg gtaattcatt ccgatagagg tagccagcat cggtctttta
     1201 tttatcaaga gctgttgaac gaccaccaca tcacacattc agtgagccaa actgggactc
     1261 ctgttgacaa tgcagtaatt gaagcgtttc atcgatcaat caaatgagaa ttaatcgact
     1321 ccaacaaata taaaagtaaa atagagatga aggtgttaat ccaagattat ttagaagact
     1381 actacccaaa caaaagaatc catacaaaat ttatgatgac tccttgacaa tttgaagcga
     1441 agaaaataag ggatctataa tatctagtgt tggtgacgcc ttctgttgcc aatacttttt
     1501 cttttttcta ataaacgcaa acagacacct taaaatcctt tagaataaag tcgacgaaaa
     1561 ccattcaaag gagtgtttca agatgtccta ttcagagttt accaaagagt tactagatat
     1621 tctagaccta aatcttatct ttcatgaaga tgcctttcgc aaagagcgaa tcaatgacga
     1681 aacatgtttt gtttttgatg gcacattgac ctatcaaccg gaggaatgct ttcatcgcca
     1741 ctatcaaaat gaacaaacga tcattaaata gggctggaaa aaagtatcca tcttattgaa
     1801 tgatgtcagt aactacaaga ccattctgcg gattaacaaa cagcgcttca aatgtaaaca
     1861 ctgtggaaag acttttttag cagaagattc agttagtgat cgtcgctgct ccattgctcg
     1921 cagagtcaaa caagccattc ttgagctatt aagcgaacct ctttctatgt ctttgatcgc
     1981 acgtatgaag cacatttctc cgaccactgt cattcgaata cttcgcagcc ttcggcctaa
     2041 aaccatttct ttgagccagc tgttaccaga agtggtgtgt ttcgatgaat tcaaatcagt
     2101 gaaaaatgtc tcgggagcca tgagctttgt catgatggac ggcaagaccc atcaactgat
     2161 tgatattgtg gaaaatcggc aactcaatcc tttacgcgac tatttcttgc gctaccctat
     2221 aaaagtccgt gaacaagtgc gtctagtggt ttcagatttc tatacaccct atcgcacatt
     2281 ggtgaaagag tgttttccga atgcccgaat cgtcgccgat cgctttcata ttagccaaca
     2341 cattggtcgg gcttttacca accaccgaat tcaagtaatg aagaccttta aaaaagggga
     2401 tcgtcggtcg aagcacttaa agaaatactg gaaactactt caaaagaatg cgtgggaatt
     2461 gaaaggtcag catcgctact ggcgtccgtc atttcgagat catctgacag aagcagagat
     2521 agtcgatcgg ctattatctt atgatgattc cttaaagcga ggctacgaag tctaccagga
     2581 ttttctttcc gttatcagaa gacaagatgt tcctgaattc gtcgcgctcc taaaagaaga
     2641 ctacaaggag ttgccagagc actaccaacc agtgttcacg accttcaaga aataccaaac
     2701 agaaatcaaa cgagcgttgc gcgttcccta ttccaacggc cctatagaat gtttgaataa
     2761 ccatatcaaa gtactaaaac gaattgccta tggctataga aactttcaaa actaccgaga
     2821 acgaatcttt ttatatcgag gaaaatattt taagaaaact aagaatacta cccaactgac
     2881 taaagccaga acaacaacac ggctagacaa gatagctgtc tagccgtgtg agtatctcga
     2941 ttattctatt gaatttcttc accaacactc attgacgaag agccaaaata agtaatctaa
     3001 ccgactgtta actaaaccat tgcaggtccc gttaaagata cttgtgcctg ttgtcaccgc
     3061 ttctttttct tgtccacaag cgcttaacac acttaataaa gcccctgcta ttattaataa
     3121 taatatcttt ttcttattaa aaacctcctc atctatttcc agattagtaa atgtagtaat
     3181 ctgtgctttc ttttttctca atactaataa ctaacttatg gggtaaacaa atactcgttt
     3241 gaccgggttt tgagatccat ccagttctaa ccgcgatttg atcaggacta ttatcttctt
     3301 taactcgaat tcttccatct ttaatctcaa caatattata ttgtcccttc gcaggatgat
     3361 aattcacgag tttatgattg atcttatcca aatcgaagcg atccatttcc ttgccatcaa
     3421 ttcgaacgat ggcatatttg atagcatctt cagttgtttc attctccttt tctatagaga
     3481 atacaaataa gggaataaaa gaggtaataa tcaaaatgcc aataattatg acatcgccta
     3541 tcttgacacc agcttttctt aaaaatttca ttaacgatat ctggctaaac atattagact
     3601 ttttccatgg aaatggtgaa atttttcgga aattatctgc attaagagaa tcagttaaac
     3661 aaccctcaag ttgttcctta atacattgtt gatccatctt cataccaata aaaactagtt
     3721 tattaatgcg ttcaccaaac aacggatgcc aggcttcctt caattcggga tttttttgta
     3781 taatcatttg acactcaatt ttaggtaaac tagctaccca ataatttaat gattcaatac
     3841 ttttatagct acccgatgtt tcaaagatcg tcgaaaaatc actatgttgg gcaaaccaaa
     3901 gaaacccttt tgctcttgta atattgccag gaaaattttt atccgtaaga tgggcaaatt
     3961 tttgtgcgtc aaggagtaac cgatttcgat agataaaact tgagatacca tattcttctg
     4021 tttcaggaat atgattttga tagccaagct ctaactcttt gaaccagccc actgaatagg
     4081 aagtctcatc tatattaaaa agatttgtat ttagaatttt cgataagtct acttcacctt
     4141 taattgtcct aattattttg gcattggact gtaacttagc aattagaccg ttaattttgt
     4201 aaatcgtctt ggttgacact aagtcactat tatttaaaag taatatgtta caacactcga
     4261 attgatcaat taacaagcca cgaatatcct gttcttcttc aactacatta ccttcagctt
     4321 ttctttcctg aatcttttca cccgtttcga aattatccca aaaacgtttt acatccacta
     4381 ctataataat ggcaccaatc tctaatcgtt gggtcaaatc aatgtctaaa gggtttttag
     4441 ctaatataat tgcttgaata attggaattg gtttgctgat tcctgaagct tcaataacaa
     4501 tttgattcaa gtcagaaaat tgcactaatt gatctagttc aacaatcaaa tcttcgcgta
     4561 atgtacaaca aatgcatcca ttcggcatcg aaacaagttt ttctccagtt cttttaaaac
     4621 ctttcttgtc gactaatacc ccatcaacat ttacttcccc tatgtcattt acaatgacag
     4681 caatttcctt tccgtgatca ccgtacagaa tatgatttag aagtatcgtt tttcctgaac
     4741 caagatagcc agttaagatt gtaataggta tttttttcat agggaacctc tttctatacc
     4801 ttgaatttac actttaagtg caacacataa aaatgagtca taatcgatat ccaactagta
     4861 taagtttgtc gagaacactc ctagaaggaa aataattatg agccagtatc gtcatcttac
     4921 tatactcgaa cgagaaagaa tctttctgct tcacgaagaa ggattttcta ttagtataat
     4981 tggcaacgac attcagcata gcccgtttac tattttacat gaactgagaa gaaacaagaa
     5041 aaaaactatt caccttccaa agcggaaaaa aattatgctt atcgaagaaa aaaatgcgga
     5101 cgttatcaca ttctaaatga tccgtgagtt tggtcaattg ttcgtcattt atttgtcgat
     5161 tttcagtggt cgccagagga aatctcgaat cgtttgaagc atgaacatat ggacttttca
     5221 attagtttta tcacgatcta acgtgcaatt tgcaatgatc ttttcgatac agaacccctc
     5281 tcacacggca ataaaggcct gatccgttca ttgcaccatc gaggaaaaac aagacataca
     5341 aaaaatcatg tcgagtctcg tggaaagatt tgccttactc attcgatcca tggccgtcca
     5401 aaatccgcag atgatagaag tgattttggt cattggaaag ccgatattgt agctggcaaa
     5461 actggtggta cttgtttagt gactttgaca gacagaaaat catgcttcct tttagcagga
     5521 aagaaggtcc ccagaaagaa agccgcttat gtgagcgaga aaatgattga acttttaagc
     5581 agtattccaa atgaggcggt caaaacgatc actccagata gaggaaaaga attctccaat
     5641 catttcgcag ttacagaagc attgaataat atgccctttt attttccaga tcctcatgcc
     5701 cttggcaaca atgaacaaat taaaatacga atagactcat tcgagaatac ttgccaaaat
     5761 cgaaagagat agctacggta gataactcaa cgataaatca gatgattgaa aaactgaata
     5821 acagacctag aaagtgttta gattggagaa caccttatga agtcttcttt gataaagtgt
     5881 tgcgcttgat ttgacaattc aagacataat aaaaagcaga tcaaatccaa ataagggaaa
     5941 aatgatctac tctggtatta tcaattaatt ggccaagcta ttatgccgtt ttgctaaacg
     6001 tctacttctt ccagtattga ccttgataaa acaacaatca ggatcccgtt ttaatactct
     6061 atgaactgtg tcaatactaa catgtaaagt tgcagcaata tcagctaatg tactcccatt
     6121 taatctcatt tgctttgcaa atcgataacg ccgataagca tcaatattct tggaatccat
     6181 agacaataat acttttctta ccatgtattt cctccaaaaa ttgaatttta atgagccatc
     6241 atttcttgag caatttcatg tttagacaat gaagctggat agaaagtagg ccaattatgc
     6301 aattcattat agagtttttc ttgactttct ccaccaaaga aaatatggaa atgctctgcc
     6361 ttacttggtg caattccatg atcactaaat tgaatatatt taaaacgacc agcacctgca
     6421 tcatcagttt caaaattaaa acgaacacca cggtttcctt tttcataatt caaaattttg
     6481 tatccaacat atttgtatgt gtattgatga tgttcaccgt tgactacaaa atcaatggtg
     6541 ttgtctttaa tgttgatagt atcaatatct gtcttatagc cagtagtata ataatctttg
     6601 tactcggcag ctgtcatgtc tttattgatt tttgctttgt aatcgaaaac ttcatccaaa
     6661 attccatctt ttaacaacgg ataaacagat tgccattcgc ctgtataatc actcaattcg
     6721 cgatccttca catcggcatc tttaaagtac ccattagcaa ctgtcttttc atccttcgct
     6781 tctttttctg gaactacctc ttcaccagca accatagttg ttttttctaa cgcttttaga
     6841 ttgtcttcca ttactgaaac ataatcttca ccattatcca tttgttcttt tgtaagactt
     6901 tcaagaggat tcagtacttc caattttatc ccagcttcgt tggccaaagt ttttgcaatt
     6961 ttatcattag cattcttttc aaagtaaata taattgatct tatttttttt aacatactct
     7021 tttaattcag ctaatcgacc agaagatggc tcttcttcag gacttagtcc agcaatagga
     7081 acttgtttca atccataatc caaagctaaa tatccaaagg ctgcgtgttg cgtcacaaaa
     7141 cttttttgtt tcgcttcttt aagactagtg gtatagtcag catccaaccg tttcagttta
     7201 gtcaaatact tttcagcatt tgtctcaaat acttttgctt ttttcggata caatttaact
     7261 aactgatcct tgatatttga aacttcctta attgccattt tgggtgaaac ccacgtatga
     7321 ggatctagtt catgatggtg atcttcttct ccgtgatcat gatcttcttc ttcactacct
     7381 ggcaataaaa tcatgtcttt tgttccttct actacattag gcccagcttt cttccaactt
     7441 tctttagctt ttggtaccca tgattccatg ttttcgttat gatatacaaa aacatcagca
     7501 tcatgagctt ttgccatatc cttagctgag ggctcatagt catgaggttc tgatcccgct
     7561 ggaaccatta aatccacgtt cgcttcatcg ccgacgatgt ttttggtgaa atcatacatt
     7621 ggatagaatg ttgttacaat ttctaatttt ccatctttat cattcgatac ttttgcatca
     7681 ttagtgctgg agcaagctcc caataatcct agagctgcta tcccaataag tcctacactg
     7741 attttcttca ttctttttcc ttccttaatt atgctaattt cttcatttta tttcgtttat
     7801 gttctttgat aatttttaat gccctttttc tagttttaat attcgggctt ttaagtcgtc
     7861 gatatgcact agatatatcc gttttctcca ttagaaatta cccccaatca ctacttatca
     7921 ttatttgctt tccgccagta atttatcaaa actctgtttg atcctttctt catccaacat
     7981 ttttccgatt agaataatag tggtttggtc tttttgtcga gtggtgtatt gaaaattaac
     8041 ctgttcgttt acctcttgca tggcaactat aaaatcctgc tcttgtaaag caatcaatcc
     8101 tttaaaacga tacaggctcc cttggttggt taaaatcagc cagtcaatcc attggagtaa
     8161 aaaatctttt tttatgtctt gatctgaagt taagagaatt gatttaaact catgtacttg
     8221 atggtgttga tgatagtatt tgccattata gtgattttct gatgaatcac tttcaaaaac
     8281 taagggatga ttaaattttt tcaaattata aaaatccttt ttttgaatcg tttcactatg
     8341 tgtgaacatt cgaatatcgg ataaagggtt aatacttttg atttctctct gtaatttttt
     8401 tagtgcagat aaattcaagt tttgttttct agacacaaaa atgcgatctg ccatgacaag
     8461 ttgcttaagg gcttgtggtt ctctttcaat aagttcaaaa ctgtcactat caataacagt
     8521 caataaacta tcgatatata ctcttcctct tagttgaggc gctgttaaaa gtgtttgaat
     8581 gattggttgg ggatctgcta tacctgtagt ttcaatgatg atttgtgaaa ttggttgttg
     8641 atgttcttct attgtttcca gtaaagaaat caatattgct aataaatcac tgcgaagact
     8701 gcaacacata catccattat ttagttgtaa cacccgttcc tcgctatgaa ctaataattt
     8761 gtgatcaatc ccaacttcac caaactcatt ttcaattata ataatttctt ccctggggta
     8821 gggtgagact tgcaaggctt gattgattag cgttgttttt ccagccccta aaaacccaga
     8881 aacgattgta actggtatgc ccattttgtc acctttttac cacttagcta tttcacttct
     8941 ttaaaaattg cccgtctgcg caattttggc gaatactttt ttaattctaa ccgttcaggg
     9001 ttattccgtt tatttttgct ggtgagatac agacgttctc cagtttcgac acattccaaa
     9061 ataatatttt gacgcataaa tcttcctcct tcaatactta tcgatcttca agatatgctt
     9121 gaatttgttg tgcatcttca aaagttaact cgccagattc caattgtgaa actaaatttt
     9181 ctttcatatc aacaggtatc tcccaataac catcgataca ttgaaataac tcctcgtcat
     9241 actcttcaaa aattggatag attcttaaaa aatcatcaaa tgataagcct tggtcctcat
     9301 agtaatcttt gactaatcca ttttcaattg cctgttgggt cggtaaaccc gtttcagtaa
     9361 taattcctct ctctaccatc gaacgataaa aagtgcattt atctagcgat tgctgggtcg
     9421 tttcaagata atactttaat ccttcttcaa ctaaaggtgg gtaaataatt tccattttga
     9481 ttccctccta aaagttatat attaaactat tttgaagctc ctttaattac tttctttcca
     9541 ttatagtacc cttttaaaga tacatgatgg ctacgtctat aatcaccatt cgcttcgtca
     9601 aatgaaattt ctggttttac tacagtttga tgagttcttc ttaaccgttt cttcgcctta
     9661 gatgtttttc ttgctggtac tgccatgata tttcctcctt ttaccaactt gcttttttta
     9721 ctccaggaat caacccttga tgggcaagtt ctctaaactt aatacgggac ataccgaact
     9781 ttcgcatata ccctcttgga cgaccatccg tctgatcacg aagcctcaga cgattcggat
     9841 ttgaatctct tggcaattta gcgagagcgg tgcgatctcc tgcggctttt aattcctgac
     9901 gtttatctgc atacttttca atcatcgcca tttgtttctt tgctttagca attttcgact
     9961 tttttgccat atacctttat actcctttta ttttgattta gacacctcaa atcgtaatcg
    10021 ttacgttttg acactgatac tttaccaaca agagttgctc ttgtcaacaa taaataacat
    10081 gaaataaaac tcatatgaat agataactat ttgaaaacga atttatcggt acatagagat
    10141 aatcaaatca tttaaaagaa tatgcagttt aggctctgaa aaataagaat tcacttttat
    10201 tagtccaatc aaacacaaat accgttttaa gtagcttttc ctctacttaa aacggcaata
    10261 tgaaagaaac tataactttt tattcaatct tttatgttta actggtttat cgccaatttt
    10321 ttaaaacttt gattattcct tatattataa gaatagacat taagtgaact aattcaatca
    10381 tactgattct ttcgtgtatt caaaattaga tgctgtttaa taaaattatt ctttttgaaa
    10441 aagaattata actatatccc aaaaattgta ggttaattta aagcctattt catcatcgtg
    10501 attgagcttc tttttcatag acgaatttct cagatagttt agttttcttt atgagatttt
    10561 tgtatatttc tgaccttcga tacaactcat tatgagtacc ttgatcaact agttttcctt
    10621 gatccatcac tacaatttta tttacctttt caatagactt caagcgatgc gatataatta
    10681 gtactgtctt atcttgtatt aaacgattta aactttcctg aatacgtttt tcattttccg
    10741 catctaaact ggcagtaatt tcatctaaaa ttaaaattgg cgcatttttc aaaaaagccc
    10801 gtgctatgga taggcgttgt cgctctcctc cagaaagtga acttccgttt tctccgacca
    10861 atgtatgata accgtcaggt agtttttcaa taaagtcttc gcagttagct aaacgagctg
    10921 cttttttcac ttcctcatct gacgcttgac tatttccaat acgtatattt tccaaaatac
    10981 ttgtattaaa caaagttacc tcttgaaaaa caatagcgat tttgctaaac aaagaagctg
    11041 gagatacacg tttaatatca tacccatcaa ttaaaataca gccttcatca taatcataca
    11101 gtctagacac taatttcaag atactagttt tcccggaacc actagcacct actaacgcag
    11161 tcacttctcc ttgtttagcc gtaaaagaga tatgatctaa aattggggta ttgttatcat
    11221 atgagaaaga gacatctcgc aactcaatgt caaaagattt cagaggacta tctgatccct
    11281 cttggatact ggtttccttc atagctctaa ttcgctgtat tttaggagct aaataaaata
    11341 tttcaagcac tgcttctttc atagaatcga aggaatcctt tatttttatt gcggctagta
    11401 agtaaccaac gacgtagagt atagtcactt cacccgttaa cagtaagtga acacctacta
    11461 gaatgactac tgctaaacta acatatgaga aaatagagga taaggccatg actgaaaaag
    11521 tacttaattc tacctttaaa tgaatccttt cactctcttc catttttttg aaaagtctgt
    11581 cttgaacttt tttagataga ttaaagctat taatctcttg ctgcaattct atagtttctt
    11641 gaaattcttc cgagttttct ctcaaagtat cgtaatattt agtattggct ttcgtttggg
    11701 atttctttga taacaagatt aagacaaaac taaataacgt tggcaaaata acagctaatc
    11761 ccattttgac attaccaacc aaaagcatca ctgaaataaa agggaaaaac agagccatac
    11821 cgccggcttt aggtattgca tgactcatcg catgctcaat accttcaacg tcagacatga
    11881 tagtttgaga taaatctgat aaattatgtt ttgaaaaata ggataatggt aaatttgaca
    11941 aatcgtctgc aatttgcact cttaaatgcg cactttcttt ataagtacta ttatataagt
    12001 tttcatactc cctatctaac aaccaataca tgaaaagcaa agtaacaaac gatacggcaa
    12061 aataaagcca acggggcttt gtattttcta aaactaattc gtctatcagc atcattataa
    12121 tgaatgctgg taccatatta atgcagtata caaaaaaact agaaatagaa gctttagtta
    12181 aagcttctgc gccctgatca cttaaagcaa atttgttttt ataaaaactt ttcattgtca
    12241 agcctccaat catttgcatt ctcataatta gtgaccaatt gtttatacac ttgatgttta
    12301 gccattaatt cattatgatt tccacgttct actattttcc cttctgacaa gacaagaatt
    12361 tcatctacag atttaatgct ggataatcga tgagctatca taataactgt tttttctttc
    12421 atcagatgtt gaaaagcctt ttgtagttca tactcattat ctggatcaat agatgcactg
    12481 gcttcatcca taataacaat ctttgcatct tttaaaattg ctctggcaat agctatacgt
    12541 tgtttttctc ctcctgacag ataaactcct ttactaccaa ttaaggtatg ctctctttcg
    12601 ggaaattttt ctaaaatcga ttcgcaaccg gctaatctta gcgcgtcaaa tactttactt
    12661 gctggcgctt gaggatttgc tatagaaacg ttatcgtaaa tactctgatt gaatagtttc
    12721 gtatcttgaa agacaaaagc aattgtgtta ataattgtgt cttttgaata gtgatttaaa
    12781 ggcttaccac caattttaat ctgtccctta tccacattgt agaaacctga aattagttta
    12841 gctatcgtac tctttccgct tcctgatttt cctactaatg cataaattct tttttgttct
    12901 aaaactaagt tgaaattttc taaaacacac tcttctttgt atgaaaagct gacattttca
    12961 aatgcaatgt cacaattttt aatctgtgtc tcgttcccat attttagttg gtcttgcttc
    13021 atttctaaat aaagggagtc tagtgtttcg actgcatatc ttgcttcaaa caagtgcata
    13081 cttacataca ttattctcat gaaagaaaca aaaataattc cacttaaaaa aaacaacatc
    13141 aacaagtcaa cagctaatat cttgggagtg gtaagagaag ttataaaaaa ggttatagga
    13201 actatcaaaa tgcaaactat gccaaaaaat agccattgaa aaagaacaaa aggtgttttc
    13261 ccactcaaag aatacttata tgcaaactta gcataatcgt taattgcttt attcaaggct
    13321 ttaaatgatg ttactttagc accaaatatc tttataactt gaattccgcg gatatattct
    13381 accgtttcag agcttaacac atctaacgat tcttgatatt tttgaataaa atttgtattc
    13441 tctgtcattt tctttaacaa gaatacacca attactaaca tggcgattag aaccaatcca
    13501 attttaaagt taatgaaaaa gcctaaggca actgataaaa caggtgttac tatggcttga
    13561 gcactatctg gaatcaagtg agcaattgcc atatgtgttt tagccgcgtt gtcatcaatt
    13621 gtctttcgaa tatatcccga agaatttaaa tcaaaaaaac gaaaacttgc ttctgttaaa
    13681 ccgctaattc ccttttttct caaattagtc tctaagcgaa acccaaggat atgagaaaat
    13741 aagccagaaa aaatataaaa taaagctcta aagttaacca agcaacagtt tgaaccgcat
    13801 aactcttagc taaatattca tctcctacaa taattagaga ctgaagaaat ttgtaaatac
    13861 tgtaatatcc atagactgtt aatacgacag aaaaatcaga aaataaagtg gctaaccatc
    13921 cgtagtattt cattttaggg acataactaa ataatttttt gtatactttc attttt
//
LOCUS       DARDHA010000064        13882 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000064 DARDHA010000000
VERSION     DARDHA010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.064"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            160..1110
                     /locus_tag="SDA10_001525"
     CDS             160..1110
                     /locus_tag="SDA10_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG4240439.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            1628..2083
                     /locus_tag="SDA10_001526"
     CDS             1628..2083
                     /locus_tag="SDA10_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG4240440.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2481..3026
                     /locus_tag="SDA10_001527"
     CDS             2481..3026
                     /locus_tag="SDA10_001527"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240441.1"
                     /translation="MELAEPGHYDEKWQNWKLESLPIFPDRYDFEVAKDKGKQFKIVA
                     ELLKKANTIIVATDSDREGENIAWSIIHNANAFSKDKTFKRLWINSLEKDVIRSGFQN
                     LQPGMNYYPFYQEAQTRQIADWLIGMNASPLYTLNLQQKGVQGTFSLGRVQTPTLYLI
                     FQRQEAIENFKKEPFFEVEAK"
     gene            3120..3452
                     /locus_tag="SDA10_001528"
     CDS             3120..3452
                     /locus_tag="SDA10_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HEG4240442.1"
                     /translation="MGNQEGIIADVQTKEKKTNSPSSFSLSSLQSKVNQLYKATASQT
                     LKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIRSN
                     NSTQILTN"
     gene            complement(3603..4582)
                     /locus_tag="SDA10_001529"
                     /pseudo
     CDS             complement(3603..4582)
                     /locus_tag="SDA10_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(4587..5354)
                     /locus_tag="SDA10_001530"
     CDS             complement(4587..5354)
                     /locus_tag="SDA10_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG4240443.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            5844..6269
                     /gene="lacA"
                     /locus_tag="SDA10_001531"
     CDS             5844..6269
                     /gene="lacA"
                     /locus_tag="SDA10_001531"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEG4240444.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            6286..6801
                     /gene="lacB"
                     /locus_tag="SDA10_001532"
     CDS             6286..6801
                     /gene="lacB"
                     /locus_tag="SDA10_001532"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEG4240445.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            6812..7744
                     /gene="lacC"
                     /locus_tag="SDA10_001533"
     CDS             6812..7744
                     /gene="lacC"
                     /locus_tag="SDA10_001533"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEG4240446.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            7747..8727
                     /gene="lacD"
                     /locus_tag="SDA10_001534"
     CDS             7747..8727
                     /gene="lacD"
                     /locus_tag="SDA10_001534"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEG4240447.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            8755..9072
                     /locus_tag="SDA10_001535"
     CDS             8755..9072
                     /locus_tag="SDA10_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG4240448.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            9072..10778
                     /locus_tag="SDA10_001536"
     CDS             9072..10778
                     /locus_tag="SDA10_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4240449.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            10920..12326
                     /gene="lacG"
                     /locus_tag="SDA10_001537"
     CDS             10920..12326
                     /gene="lacG"
                     /locus_tag="SDA10_001537"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEG4240450.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            12349..12621
                     /locus_tag="SDA10_001538"
     CDS             12349..12621
                     /locus_tag="SDA10_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEG4240451.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            12642..13541
                     /locus_tag="SDA10_001539"
     CDS             12642..13541
                     /locus_tag="SDA10_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEG4240452.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         4503 a         2322 c         2695 g         4362 t
ORIGIN      
        1 cattagaagc tggagacaag attgtctaga taatgtgtga gataaaattt ttggcaggtt
       61 atcatacata agacaaaatg tttttaaaac tgataacgta tttattccac aacaattacg
      121 catttatttc tgagaaaaat ataaagcagg aggattacaa tgctgaaaac ttattcctta
      181 acaaaagata cattattagt ttcgtcagaa tcaaatatca aaaattcttc tgttgtttat
      241 gtttatggtg acgaacgaga atatatcgaa tcttctgaac ataaatatga ctttaaaata
      301 ggcaatatac taacgtttga tgatcgagtt gcatatgaca cgatgataga taagaacgat
      361 gaaaaaagtt tgctcgtaag ttttttgttt cccgaggtac atgaaggtgg gcatctagat
      421 accctgacga catctgttgt tttcttggta tttaaaaata agttgataat ttttacaaaa
      481 caaaaattaa aattcattcc cgaactctta tccaatatgg ttttacgtga tgtcaatcat
      541 ttcgtggagg aagtactttt aaagatcatg caaaaagatt tctatatgat gcttaacgac
      601 cttcgtggag tcaaggaaaa aattgatgat ttagactcag aaatatcaac gtccggttca
      661 ctcaaaccaa cgtttggtga tttattgact cttcaaaaat acatgatagc tttgtcaaca
      721 acttataaag ctaaccataa agcgcttgat ttcattaaaa aaaatttcac aactattgac
      781 gatattgatt ttagtactaa aaaaataatt gatgatttat acgatacaag tgatacaatg
      841 gatagaatca ttttaagtta cagccaatat ttggacaaat tggaaaatat gataaacaat
      901 atgatttcgt atcagctaaa catgattatg aaaaccctaa ccgagatatc aattgttttg
      961 actattccag caattatttt tggcttttgg ggaatcaatg tccatgtccc gtttgaaaag
     1021 tcttcttatg gtgttttagc cgtattgatt atctctgcta tactaagtct aatttgctgg
     1081 cttttgatgc gacgcaaaac atatttgtaa agtatttgtg ataccctaat atgatttaaa
     1141 gcagaatttc tttgttgact ttttaaaagg ctgattcctt tcaataattt aatagcttca
     1201 gcggctgctc agaagtcaat aaattggact atttttattc agttacttag gcaacatttg
     1261 agatacgatt gcgattataa aaattgatat accaaccaat ggctgcttgg aagactgaca
     1321 tattagcctc gatatcatcg aataactaaa tacagaaaaa tacaataaat attatcaaac
     1381 tagggactat actagaaaac atcagtacat tccttaactc agggtctata attagaaaat
     1441 cgcccctttg aaaactgatt aaaacagccc ccattatcta gtgactagat aatgggggct
     1501 gtttttttgt taagtgatat aataaaccat aaaagaagtt tagaaatacg gtcctggtca
     1561 tcaataaata aacaatttaa ttaattattt atttaattat ttaatttcag aaaggtatac
     1621 atgaatcatg gtgcaacaaa ttgtcctacc catcaaagac tccagcgtct taaagatggt
     1681 acaagataca cttctcgata gtttccgtgc tggtcgccga aattacaccg tttttcaagt
     1741 tggtaaggcc acgctactac gtgtgagtga tgtcatgaca ttaaaaaaat cagatgtcta
     1801 taatccagac ggctctgtca aaaatgcaac ctttattcat gataaaaaga ccggtaaagc
     1861 aaacacgtta tatttaaagc ctgttcagca agacttgttg caatatcatg attggctggc
     1921 ccaagaaaat atcaattctg agtggttatt tccctcgaca gcccattatg atcttcatat
     1981 cactgagaag cagtttcata aggtgatggc gcatgttggt gatctcttag gaatcaactt
     2041 ggaagacaaa gagaaaaaat tgcgagtcag aaaaccgttt tgagatatga acagcatttg
     2101 ccttcacgcc ttagcgattt taaataatag ccttgaaagt gattatggaa aagccctcta
     2161 cgctcaatgc aaaattgacg tagaaactat ttttgatcga ttgaaaggtg tatttggtat
     2221 gtgaagaacc catgtcagag gcaagaacgt tgcacctact gacattggaa tgatgcttat
     2281 gagcatgaat ctaaccaaat tatcccttga ggctagaaga aaagcggaag cttttcaaca
     2341 caaagcagcc aaaaataaaa aacgcgacga agcaatcaca tttatgatta ttcatcgcgt
     2401 tttttatatt tagagctagt tatttgctag acccactatt tacagatgaa acgtttatca
     2461 cctttggttt tgggcattta gtggaattag cagaaccagg tcattatgac gaaaagtggc
     2521 aaaattggaa acttgaatct ttgccgattt ttcctgatcg atacgatttt gaagttgcaa
     2581 aagataaggg aaagcagttt aaaattgttg cagaacttct caaaaaggca aatacaatta
     2641 ttgttgcaac agatagcgac agagaaggtg aaaatatcgc ctggtcgatt atccataatg
     2701 caaatgcctt ttcaaaagat aaaacattta aaagactatg gatcaatagc ttagaaaaag
     2761 atgtgatccg aagcggtttt caaaatttgc aaccaggaat gaattactat cccttttatc
     2821 aagaagcgca aacacgccaa attgccgatt ggttgatcgg catgaacgca agccctttgt
     2881 atacgttaaa tttacaacag aagggcgtac aaggtacatt ttcactagga cgtgttcaaa
     2941 cgcccacctt ataccttatt tttcagcgcc aggaagccat agagaatttt aaaaaagaac
     3001 cttttttcga ggtggaagct aagtaaaaca agggtcattt aagggcgttc taagccccac
     3061 acagcgtttt aaaacccaag aggagctttt tagcttttgt ttcttctaaa caagctaaaa
     3121 taggcaatca agaggggata attgctgatg ttcaaaccaa agagaagaaa acgaatagtc
     3181 cgagttcgtt ttctttaagt agtttgcaat caaaagtcaa tcagctttat aaagcgacag
     3241 cgagccaaac tttaaaagct atgcaaggac tgtatgaagc aaaattattg agttatccaa
     3301 gaacagatac accatttatt acagagaacg aatttgctta tttaaaagcg aattttggca
     3361 aatatagcgg ttttttagga cttgatcttg aaatgttgtt atcattgata atgatacgtt
     3421 caaacaacag cacccaaatt ttgacgaatt agtgaaactc tatgaaaaag acgtagtaaa
     3481 acatgcaaca tcctattcta atcagctggt aaagcttaat taggttatat aagcttgaaa
     3541 ataaagggtt tgtccaatat ggacaagcct ttttcttatg ttttgccaca gatttgccac
     3601 acttattgtg taagagtatc aaggataggt ataatttgat actttgtttt tttagtgatg
     3661 tgcaaataaa tttctgttac acgactacct ctagaatgcc ctacacggtc ttgaattgct
     3721 tctaggggta ttccttactc tgctaagaaa ctaatatgcg tatgacggaa tatatgggtc
     3781 gtaatatgtt tattaaaact aagtttatta tcaatatcaa ttcctgctcg ttttggatta
     3841 ccttttcgat ttaattttcc attttcaggt gagttgagga aattgttaag gacactataa
     3901 tgaagaggtg ttccattata gtcgggttta tgacgacttt tggaagtaaa aatccatcca
     3961 ttatctttat aatcaggatt gaatctcata tgttggtgat ttaactcaat ttcctcttta
     4021 agaatttcta tagccctttt agataattgg acatttcggt aactttgtaa gttttttaca
     4081 ttaccttcat agctattttc tgcagctcct ccgtgagtgg actgactaat gatgatattt
     4141 tgcttttcaa aatattgata ggcaccaata ttttggagtt tcgactctgt aagtcaacag
     4201 ggccattagt tagaatacct attgggatgc ggtttttatg tagagccagc agtagttgat
     4261 gtatctcagg aatcatcgta atctgatcga gttcatattc atagtcagct tggaagtcta
     4321 gagcttctgt cctagaaata ctatcgatgc caaattcttc catagtcttc tgacagcgaa
     4381 aaattctaag ctgctcaata ctgatgagtt ttttcgtgta tttaggaaaa gcaacatcag
     4441 accaatatct gaaacgtttg taaatgttat ctatttgttg gatatcaatc gttggaaaac
     4501 atttctcaac gctacgttta aaggggagtt gatggtcata aagagtgtca tcaaggtcaa
     4561 aaacaatatt tttataagtc ataattctag tccgctttta aaatagtagt gtactggcta
     4621 aattcctcta agtgttgagg gctaatctgg ttatctgtaa tgatggtatc gagttgatct
     4681 agattataga agttaaagaa atcgtatcga tcgaatttag tactgtctac taataagaat
     4741 ttttctacag cattgtttag ggcaagttgt tgaatcacac cttccttgtc actatatgta
     4801 gcaatagaat tatgggtaac agcatttgca ctaacaaaag ctttggcaaa tctaattgct
     4861 tttaaatttg tcgaagccat tgaacctaca aaagctccag ttatttctct atattcaccg
     4921 ccaagaagca ataaatcaat ggtttcgcta tcatttagaa tcaagaacac agggagacta
     4981 tttgtaatga cacgaatttt ataaccttta cgacctttca attctaatgc cagttgttca
     5041 agtgtagttc caggtccaat aaaaatagta tcgccatctt tgattaaaga gcaggctttt
     5101 ttagcaatgt caattttttc ttttgtattt agacttttct tctcgatatg tgtcttttca
     5161 agtggctttt tagaggaaag caattgtgca ccaccatgag tacgtgttaa aagtccatca
     5221 gcttctagag catcaaggtc tctacgggct gtcatatcgg aaatatctag ttcatctatt
     5281 atttctttta tggttatggt cccatcaatc tttaacatat ctaaaatttt ttctaatcgt
     5341 cgttttttgt tcatatgaag acccttcttt cataaagtaa tttttttcca aagataattc
     5401 tcttttaatt gtatcataaa agacaatatt ttcaaggtaa aacaaacaat ttcaaacaaa
     5461 aacaaatgtt agatgatgaa ataagaacag aggattgacg tatattagct taggtcagat
     5521 tttgtataag acgaaaataa agtaggacct cttaatcagt aagttataga aagtaaaaga
     5581 cttttgtaat acttgaatag atatttcacg tccattttgt gatggattaa atgaacaaaa
     5641 atgaacaata atttaacggt gttatctatt ttttaaaaaa acaaataaaa aaaacaaaaa
     5701 ataaacaaaa aaagtgttgc tttttgtttg aatgcttgat attatataaa caaagaaatg
     5761 atgaaaacgt tttttcgaac attttgcaaa atgttttcta cttctacgta gcatttcttt
     5821 ctaaaattta ggaggtggtc aaaatggcta ttgttgttgg tgcagatctc aaaggtacga
     5881 gattaaaaga tgttgtgaaa aattttcttg tagaagaagg ttttgaggta attgatgtga
     5941 ctaaggacgg acaagatttt gttgatgtta ccttggcagt tgcctctgaa gtaaataaag
     6001 atgagcaaaa cttaggtatt gtaattgatg cttatggagc tggtccattc atggtagcaa
     6061 ctaaaattaa aggtatggtt gcagcagaag tttcagatga acgctcagct tatatgacac
     6121 gtggacataa caattcacga atgattacgg ttggcgctga aatcgtcggt gatgagcttg
     6181 ctaaaaacat cgctaaaggt ttcgtcaatg gtaaatatga tggcggacgt catcaaatcc
     6241 gagtagatat gcttaacaaa atgtgttaag aaaaggagtt aaggaatgag aatcgcaatt
     6301 ggatgtgacc acattgtcac agatgaaaaa atggcagtat cagaattctt gaaatcaaaa
     6361 ggacatgagg tccttgattt cgggacatat gaccacgcac gtactcacta ccctatctat
     6421 ggtaaaaaag tcggtgaagc agtagtaagt ggtcaagcag acttgggagt atgtatctgt
     6481 ggtacaggtg tcggcattaa caatgcagtc aataaagttc caggtgttcg ctcagcgttg
     6541 gttcgtgata tgacatcagc tatatatgca aaagaagagt taaatgcgaa tgttatcggt
     6601 ttcggtggaa agattactgg tggtcttctt atgaatgaca tcattgaagc tttcattgaa
     6661 gctgagtaca aaccaacaga agaaaataaa aaattgattg caaaaatcga acacgttgaa
     6721 acacataatg cacatcaagc agatgaagat ttctttacag aattccttga aagatgggac
     6781 cgtggtgact accacgatta aggtataaca aatgattctg acagtcacac taaatccttc
     6841 agtagacatc tcttatcctc tagaaacatt gaaaattgat acagtgaata gggtgaaaga
     6901 tgttagtaag acagctggtg gaaaaggctt aaatgttact agagtcttgt atgaatctgg
     6961 tgataaagtt actgctacag gtttcttagg tggtaaaatt ggtgaattta ttgaaagcga
     7021 attagaacaa tctcctgtta gtccagcttt ttataaaatc tcaggaaaca ctagaaactg
     7081 tattgctatc ttacacgagg gtaaccaaac agaaattttg gaacaaggac caacaatttc
     7141 tcatgaagaa gcagaaggct ttcttgacca ctatagcaat cttattaaac aatcagaagt
     7201 tgtgacaatt tcaggaagtt taccttcagg acttcccaac gattattatg aaaaacttat
     7261 ccaacttgct tcggatgaag gagtagcagt cgttttggat tgttcaggtg cacctcttga
     7321 aacagttcta aaatcgtcgg ctaaacctac tgccatcaaa cctaataatg aggaactttc
     7381 acagttgctt ggaaaagaag taacaaaaga tattgaagaa ctcaaagacg ttcttaaaga
     7441 gtcacttttt agtggtatcg aatggatagt ggtctcatta ggtcgaaacg gtgcctttgc
     7501 aaaacatggt gatgttttct ataaggtaga tattcctgat atcccagttg ttaatccagt
     7561 cggatcaggg gactcaacgg tagctggtat tgcatcagct ttaaatagta aaaaaagtga
     7621 cgctgactta ttaaaacatg cgatgacatt gggtatgtta aatgctcaag aaacaatgac
     7681 agggcatgtg aatatgacta attacgaaac actaaactca caaattggag taaaagaggt
     7741 ataaaaatgg tacttacaga acagaaacgc aaatctttgg aaaaaatttc agataaaaac
     7801 ggtgttatct cagctttggc atttgaccaa cgtggtgctt tgaaacgttt gatggcacag
     7861 tatcaagata cagaaccaac tgtagctcaa atggaagaac taaaggtttt ggttgctgat
     7921 gaacttacaa aatacgcttc ttcaatgttg cttgaccctg aatatggact tccagcaaca
     7981 aaagcattgg ataaagaagc aggtcttctt cttgcctatg aaaaaacagg ttatgataca
     8041 tcaagtacaa aacgtttgcc tgactgcttg gatgtttggt ctgcaaaacg tatcaaagag
     8101 caaggcgctg atgccgttaa gttcttgctt tactatgatg tagacagctc agatgaactt
     8161 aaccaacaaa aacaagctta tattgaacgt gttggttctg aatgtgtcgc tgaagatatt
     8221 cctttcttct tggaaatcct tgcttatgat gaagaaatct cagatgcagg ttctgttgaa
     8281 tatgcaaaag tgaaacctcg caaagtcatt gaagcgatga aagttttctc tgatccacgc
     8341 tttaacattg atgttcttaa agtagaagta ccagttaacg ttaaatatgt cgaaggtttt
     8401 gctgatggtg aagtagttta cagtaaagct gaagctgctg acttctttaa agcacaagaa
     8461 gaagcgacta accttccata catctaccta agtgcaggtg tatctgctaa attgttccaa
     8521 gaaacattgc aatttgctca cgactcaggt gccaagttta atggtgtgct ttgtggacgt
     8581 gctacttggg caggatctgt agaaccttac atcaaagaag gtgaaaaagc agcacgtgaa
     8641 tggttgcgta ctactggatt cgaaaatatt gatgaactta acaaagttct tgttaaaaca
     8701 gctagtccat ggactgataa agtatagtaa aaacataaaa cggaggatat tgttatgaac
     8761 agagaagaga tgactctctt agggtttgaa attgttgctt atgctggaga tgctcgctct
     8821 aaacttttag aagcgcttaa agcggctgaa aatggtgatt tcgctaaggc agatagtctt
     8881 gtagtagaag caggaagctg tattgcagag gctcacaatt ctcagacagc tatgttggct
     8941 cgagaagctt ctggggagga acttccatac agcgttacca tgatgcatgg tcaggaccac
     9001 ttaatgacta ctgtcttatt aaaagatgtg attcatcacc tcatcgaact ttataaaaga
     9061 ggagcaaaat aatgcataaa ctcattgaac ttattgagaa agggaaaccg ttctttgaga
     9121 aaatctctcg aaacatctat cttcgtgcta ttcgtgatgg atttatcgct ggtatgccag
     9181 tcatcctttt ctcatctatc tttatcctta ttgcctatgt cccaaatgct tggggattcc
     9241 actggtcaaa agacattgag accttcttga tgactcctta tagctattcg atgggtatcc
     9301 tagcattctt tgttggtggt actaccgcta aagctttgac ggactcaatg aaccgtgacc
     9361 tacctgcgac caatcagatt aacttcttat ctacgatgct agcgtctatg gtagggttcc
     9421 ttttgatggc agctgagcct gcaaaagaag gaggcttctt gacagccttc acgggaacaa
     9481 aaggtctttt gacagcattt attgcagcct ttgtgacagt taatgtttac aaggtctgtg
     9541 tgaaaaataa tgttactatt cgtatgcctg aagaggttcc accaaatatc tctcaagtat
     9601 ttaaagactt gattccattc actgtagcgg ttgttctcct ttatggattt gaactcattg
     9661 ttaagggaac tcttggtgta accgttgcag aatcaatcgg tacccttctt gctcctctct
     9721 tctcagctgc agatggttac cttggaatta cacttatctt tggtgcttat gccttcttct
     9781 ggtttgttgg tatccacggt ccttcaattg tcgaaccagc aatcgctgct atcacttatg
     9841 ccaatatcga tgccaacttg catcttatcc aagctggaca acatgcagac aaagttatca
     9901 cttctggtac tcaaatgttt attgttacca tgggtggaac aggagctaca ttgattgttc
     9961 cattcttgtt catgtggatt tgtaaatcag aacgtaaccg tgccatcgga cgtgcctcag
    10021 ttgttccaac attctttggg gttaatgagc caatcttatt tggtgcacca atcgtattga
    10081 atccgatttt ctttgtaccg ttcattttcg ctccgattgt caacgtttgg atctttaaat
    10141 tctttgttga cacattgaac atgaactcat tctctgccaa ccttccatgg gttactcctg
    10201 gtccgttggg aattgtactc ggtactaact tccaagttct atcatttatc ttagccggac
    10261 tcttggtagt tgttgatact atcatttatt acccatttgt taaggtatac gatgaacaaa
    10321 ttcttgaaga agaacgttct ggtaaaacaa acgatgctct taaagaaaaa gtagctgcaa
    10381 acttcaatac tgctaaagca gatgccgttc ttggaaaagc aggcgttgct aaagaagatg
    10441 tagcagcaaa caataatatc acaaaagaaa ctaatgtcct tgttctttgt gcaggtggag
    10501 gtacaagtgg cttgcttgcc aatgccttga acaaagcagc agcagaatac aatgttccag
    10561 tcaaagcggc agcgggtagt tacggtgctc accgtgaaat gttgccagag tttgacttgg
    10621 ttatcttggc tccacaggtc gcttcaaact tcgatgatat gaaggctgaa acggataaat
    10681 tgggcattaa acttgcaaaa actgaaggag ctcaatatat caaattgaca cgtgatggac
    10741 aaggtgctct tgcatttgtt caacaacagt ttgattaata aatttgtaaa agttatttga
    10801 gacgagatta ttctttcaat tccttataat attgttaacc atatcattgc atttcatttc
    10861 cttggtttcc ggaactcaga atatcgtctc aaatgctttt tttgaaagga tttatagtta
    10921 tgactaaaac acttcctaaa gattttattt ttggtggtgc tacagctgct tatcaagcag
    10981 aaggtgcgac tcacacggat ggtaaaggcc cagttgcatg ggataagtat cttgaagaca
    11041 attattggta cacagcagag cctgcaagtg atttttacca taaatatcct gttgatttag
    11101 aattagctga aaaatatggc gttaatggta ttcgtatttc aattgcctgg tctcgtattt
    11161 tcccaactgg ctatggcgaa gtaaatgaaa aaggtgttga attctaccat aatctttttg
    11221 ccgagtgcca caaacgtcat gttgagccat tcgtgacttt gcaccacttt gacacaccag
    11281 aagttcttca ctcagatgga gatttcttaa accgcgaaaa tatagaacac tttgtagatt
    11341 atgccgcttt ctgttttgaa gagttcccag aagtaaacta ctggacaact ttcaatgaaa
    11401 ttggcccaat tggagatggt caatacttgg ttggtaaatt ccctccaggc attcaatatg
    11461 atcttgccaa agtcttccaa tcacaccaca atatgatggt ttctcatgca cgtgctgtga
    11521 aattgtataa agataaaggt tataaaggcg aaattggtgt agtacatgcg ttgccaacca
    11581 aataccctta tgatccagaa aatccagcag atgttcgtgc cgctgagtta gaagatatca
    11641 tccataacaa atttatcttg gatgcaacct accttggaca ctattcagat aaaacgatgg
    11701 aaggggtcaa ccacatccta gctgaaaatg gtggagaact tgatcttcgt gatgaagact
    11761 tccaagcact tgaagcagct aaagatttga acgatttcct tggtatcaac tactacatga
    11821 gtgattggat gcaagccttt gatggtgaga ctgaaatcat tcacaatggt aagggtgaaa
    11881 aaggaagttc taagtaccag attaaaggtg ttggtcgccg aatcgcacca gattatgtgc
    11941 ctagaacgga ttgggactgg attatttatc ctgaaggctt gtatgaccaa atcatgcgag
    12001 tgaaaaatga ttatccaaac tacaagaaga tttacatcac tgaaaacggt cttggataca
    12061 aagatgagtt tgtagataat accgtttatg atgatggtcg tattgattac gtgaagaaac
    12121 acttggaagt tttatcagac gcaattgcgg atggtgcaaa tgttaaaggt tacttcatct
    12181 ggtcacttat ggatgtcttc tcatggtcaa acggatacga aaaacgttat ggactattct
    12241 atgtagactt tgacacgcaa gaacgctatc ctaagaaatc agctcattgg tataagaaag
    12301 tagcagaaac tcaagtgata gagtaatatt acttgaaaat gtaaaaaatt gttatccgat
    12361 actttagaaa aagtacaaca cttatttaga ccaggctttg aaaaggtata tattgttagt
    12421 ttcgaagatt gtcctatgat tcctgagctt gaagctaccc cgttgctaaa atgtggtaaa
    12481 tggtatgttt caactggaaa agagtggatt tgccattctg atttagagtt atcagcattt
    12541 gaatgggaat ttttacaatc actcgatgta gaaatacgtg agactattca cttcgaggta
    12601 aattatttgc ctttccaata ataaaaagaa aggaattagt tgtgactatc gaattaaaaa
    12661 acgagtatct tactgttcag tttaaaacac ttggtggtca actgacttcg attaaagaca
    12721 aggatgggat agagtatctc tggcaagctg atccaaatta ttggaatgga caggcgccta
    12781 ttctatttcc tatctgtggt agtttacgaa atgattgggc tatttataga cctcaagaaa
    12841 gacccttttt tacaggtctt attcgtagac atggttttgt tcgaaaagaa gaatttattc
    12901 tggaagaagt caatgataat agcgtgacct ttagtattaa accaaatgct gagatgcttg
    12961 ataattacct atatcagttt gaactgagag ttgtttatac cttgaatggt aaatcaatta
    13021 gaaccgaatt tcaggtaacg aacttggaaa tggaaaaaac aatgccttac tttattggag
    13081 cccatccagc attcaatagt cctttagtag aaggtgaaaa ctatgaggat tattatattg
    13141 aatttagtga ggttgagtct tgctcgatac caaagagttt tcctgagaca ggattattag
    13201 atttgcaaga tcggacacct tttttggaaa atcaaaaaat tctcgatttg gactactcac
    13261 ttttttccca tgatgccatt acacttgacc agctcaagtc tcgtagcgta acgcttcggt
    13321 caaggaagtc tggtaaaggt cttcgagtag attttgatga ttttccgaac ttgattctgt
    13381 ggtctacaac taataaaagt ccattcattg ctttggaacc ttggagtggt ctctccactt
    13441 cacttgaaga aggtaatttc ttagaagata aaagacaggt aacgaaaatt tcacctcaag
    13501 aaacttctag aaaaatctat gatattacta tccttaatta aaatttgtta ttatttgagt
    13561 cttcaataag atttgaatac tcttaccttg tgatgataga ttggtcttct ccgtgccagt
    13621 cttacatcgc aaggttttta agttttttgt gggtctatgt caactgtagt gggtgacgaa
    13681 aagctaatat ctaaagacag tctggtagta aaaaataaga ttatttacta cttcttgatt
    13741 taaaataata gtggagccat taatttgtgg caaatttaat ttaatatttt gcctcaaatt
    13801 agccacactt tttatagaac taagaggaaa aatgaaattt tcttcctatt aatatagttg
    13861 tttgaaatct taaatatagc gt
//
LOCUS       DARDHA010000065        13841 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000065 DARDHA010000000
VERSION     DARDHA010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.065"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..147)
                     /locus_tag="SDA10_001540"
     CDS             complement(<1..147)
                     /locus_tag="SDA10_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4240453.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNF"
     gene            complement(626..1345)
                     /locus_tag="SDA10_001541"
     CDS             complement(626..1345)
                     /locus_tag="SDA10_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEG4240454.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1394..3040)
                     /locus_tag="SDA10_001542"
     CDS             complement(1394..3040)
                     /locus_tag="SDA10_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG4240455.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3219..3809
                     /locus_tag="SDA10_001543"
     CDS             3219..3809
                     /locus_tag="SDA10_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG4240456.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3901..5406
                     /locus_tag="SDA10_001544"
     CDS             3901..5406
                     /locus_tag="SDA10_001544"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEG4240457.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5490..6842)
                     /gene="celB"
                     /locus_tag="SDA10_001545"
     CDS             complement(5490..6842)
                     /gene="celB"
                     /locus_tag="SDA10_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEG4240458.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6842..7360)
                     /locus_tag="SDA10_001546"
     CDS             complement(6842..7360)
                     /locus_tag="SDA10_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240459.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7376..7702)
                     /locus_tag="SDA10_001547"
     CDS             complement(7376..7702)
                     /locus_tag="SDA10_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEG4240460.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7715..9694)
                     /locus_tag="SDA10_001548"
     CDS             complement(7715..9694)
                     /locus_tag="SDA10_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG4240461.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9715..10029)
                     /locus_tag="SDA10_001549"
     CDS             complement(9715..10029)
                     /locus_tag="SDA10_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEG4240462.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10197..10490
                     /locus_tag="SDA10_001550"
     CDS             10197..10490
                     /locus_tag="SDA10_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEG4240463.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10568..11680)
                     /locus_tag="SDA10_001551"
     CDS             complement(10568..11680)
                     /locus_tag="SDA10_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG4240464.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            11881..12057
                     /locus_tag="SDA10_001552"
     CDS             11881..12057
                     /locus_tag="SDA10_001552"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240465.1"
                     /translation="MITEVSYETLQKKKTLIVAAVLFPAPCVCRNCGSAVVDGNRKLM
                     VVKNKKKETIVHFE"
     gene            12067..12246
                     /locus_tag="SDA10_001553"
     CDS             12067..12246
                     /locus_tag="SDA10_001553"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240466.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12440..12610
                     /locus_tag="SDA10_001554"
     CDS             12440..12610
                     /locus_tag="SDA10_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4240467.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(13034..13783)
                     /locus_tag="SDA10_001555"
     CDS             complement(13034..13783)
                     /locus_tag="SDA10_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG4240468.1"
                     /translation="MVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQ
                     SGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENR
                     LTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQL
                     VETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPK
                     TGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4321 a         2824 c         2367 g         4329 t
ORIGIN      
        1 aaaatttttg aatgctacga cattcgccat tacaggtatg taaaagaaaa aaatcatcca
       61 aagcataccc ggtacagcca tcaggacaag tgctttataa cgccatagat tcataaaaaa
      121 atgattgaac ccctttcgtt ttttcatcta aaaacccctt tcgattcatt cgcaacgtaa
      181 ttatagcgct ttcaataatg agaataaata aacaaagtat agggaaaatg aaacaaagta
      241 taggagaggt tgtgaaatca gcgttttggc ctgagtatta gagaaaaaaa cgggaaaata
      301 gttcctcata ttttgccgtt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt
      361 tatcagaggt tgtgaattca gcgttttggc ctgagtatta gggagatcgt gtccttacta
      421 tctaactctg agtcacaagg aacaatgggg actgtaacaa aagtcttgtc acagtccctt
      481 attccgaaca aacggtgttc cagaagtagc ttctacggaa ataagccgaa atttcacaaa
      541 aaagccatcc gaaagtcaga cggacaagtc tgcttttcag atggcgctac aactactctg
      601 cttattcttt tagctatcca ttcaattatt tttctctcac ttgttctagc tcttctttgg
      661 tcatgggtcg atattgccct ttattcaaat ctttaggcag ccaaaatttt cccatacgga
      721 ttctttttaa atataagact tcttttccta ctgctttgac cattcgctta acttggtgaa
      781 actttccttc atgcaaaatc agacggataa ttgaagtttc tgtctcttga tctacttgat
      841 caatttggag ttttgccggc aatgtctgct catcgccatc aataatcagt ccttctgcaa
      901 ataaacggat atcttcttct gtcattatac cgctaacctc agctagatat tctttctcca
      961 catgtttttt aggtgatagt aactgatggg caaacacccc gtcattcgtt aaaatcaaga
     1021 ggccctctgt gtctttatct aatcggccga ctggaaaaag atcttcccga taatcctggt
     1081 cttccaataa atcgattacg gtttgatcat gattatcctg agtagcagaa atgactcctg
     1141 caggtttatg caaaatgtag taaaaatctt tttgatacgt aatgatttct cctaaataag
     1201 taaccgcatc ttcgaattca tttacttgcg ctttatcgct tttcaccact tgtccattga
     1261 cttcgatcgt tccttttttg attaatcctt tgacttcttt gcggctgcca agtcccatat
     1321 ctgctaaaaa cttatctaaa cgcatgcgtt tcccccattt caaaagagca tcaatctagc
     1381 tcttctgatt cacttatttt atttttaatt tatgtcgtaa acgcgctatt cctggtccta
     1441 gtagtctttc tgccaagcgg attttcaaag ctaaataacc atacactaaa gcccctatac
     1501 ctgctactac aagaatcaac aatagagact ggaatttgct atcttcgttt aaaaaagcac
     1561 caaaaatcat ctttgtgatc cacgaagcca aaagcataat caaggtaaga ataaagatca
     1621 gcaatgtcct gcgccagaca aaacttggtt taaagcgggc tacttcatgg attctcttta
     1681 agatcaaggt acaagacaca gcaaatccga tcattgtcgc caataaaggg ccgtatactt
     1741 caaataaacg aatggctggg tattgaagaa ggagtttcac tagaaaccca ataaccaaat
     1801 aattaattgc cgccttgttt tcaaacattc cttgcagcat gttggaaact agcatatata
     1861 gtcctaggaa gagtcctaca aaacttgctt ggatcagtac attacttcct agagaatccg
     1921 gtgtataaaa taatgtattt agcggatagg caagtaacat aacaccaaat gtcgccggaa
     1981 acataaaaaa tgaaaacaac tgtaaatttc cgcttgtcaa ttttgccaga cctactcgat
     2041 ctttcaacgt aacagcttct gtaatcaacg gtaacccagt agacgcgatc gatgttgcca
     2101 atgcaataac taccatcgtc aacttatctg gattggcact aaaaatcgaa aaaagatcca
     2161 gtaactgtgc attggaatac tcggtggatg cagacatgat cttcataaaa gtgaactgat
     2221 cgactaattt gaagatcgtt acccctgaac ccacaataat aaacggaatc gcttctttaa
     2281 cggtatctaa caacaattcc ttcgttgcaa tatttatttt gttttcgctg tagtgaataa
     2341 acgccgatgt atacgcttga tgtttcttca aaaaatacaa aaggacaaga atacttgcta
     2401 gcataccgat aaatgccgcg aaagttgatt gcgtgaccgc cgtcacatag tcaccatcca
     2461 tgactttcat gatgatgaat gtagccaata gcatataaaa aatacgtgcc acctgttcta
     2521 caatctgcga cagcgcataa ggcatcattt cttgattgcc ttgcaaatac cctcgtatca
     2581 cactcataca aggaaaaacc aatacagcga tactcaaaga acgcatcgtt ggaatcagtt
     2641 cttctccgcc accagaagca tgagctagcc aaggagaggc gatgtacatg aacaaagcaa
     2701 acaacaggcc taacccgccc atcatctgta atgcacggat aaataatcgt cgtgaagtgc
     2761 catattcatt caatgaatta tagcgtgcag tttgttttgc aatcgcagca ggtattcctg
     2821 ctgtagagat cagtagaaac aatgcatata tattgtatcc catattgaat aatccattgg
     2881 cagccttagc attttcgccc atccaagcat accagggaat gatatagaca gcgcctagca
     2941 atcttgaaat gatattactg gcggtcatcc aagcagagcc tcgtgccatt ttttcttgcg
     3001 gtgttaattg atctaatgct ggttttcttt gattatccat cggtcagatt ccaactttct
     3061 acaaaacatt actatcattt taattggtaa aacagaaact ggcaatcgat tttatataac
     3121 ttttataaga acaattgttt ttttgaattc ggtttcgttt ttttggtaaa gtaaagaaat
     3181 atacagcctt ttatggctaa catttatagg agatatcaat gacacaagaa ataaaaaaac
     3241 tacccgttat atttttaggt cttttcttta tcacagttgg attgaattgg ttcttgctcc
     3301 ctcacgatat agcttccgcc ggagtgggcg ccatcggaca tttagttgaa acggcctttt
     3361 ctatccaacg ctcagttact gtttggagca tcaatcttat gatgctgctt ctagccgctg
     3421 tcctattagg aaaatcagta ttttttaaga ctgtagcggg aagtctgctt tttcctgttg
     3481 ttttagagat tgttccaaaa gtggaaatga tctcctcaca tttcttttca ctgatttcag
     3541 gaagtctttt gttcagtctt ggggtttaca cattgtacac agtcggggcc tcaaatggcg
     3601 gtataacgat accgccgatt attttccaca aattttttcg gcttccaatg ccgcaaggac
     3661 ttttacttac caatagtctt atcgtctttt tgaattatta tgtttttgga ttgttggaga
     3721 cactattcgt tctcctatct attacagtca gttcattgtt tatgaaaata ctcatccgct
     3781 tgtcccctgt gtcaagaaaa tttgaataaa gctggatttt gtcatagcgg aaacaaacga
     3841 tatattaaaa agagtgtgct ataataaacc aagaaattga ataaagcgag ggatcaatca
     3901 atgagcttct ctttagaaga catccgtcat ttactattaa aagaaaatct attaaaagaa
     3961 tttgtttcaa caagaggctg gcatcttgat gtaccagaaa aagctgaatt tcttcagtta
     4021 agttatgatt caagaaaagc tgatgcttct actttgtttt tctgtaaagg aatgaatttc
     4081 aaagaagaat atttggattc agcaatcgaa cagggtattc aatattatgt atcagaacaa
     4141 ccatacgaaa attcagcagt aggaatcatc gtaacggata tccgtaaagc aatggcgctt
     4201 cttgctatgg ctttttacga ctatccgcaa aataaattga aagtgatcgg attcactgga
     4261 acaaaaggta aaacaactgc tgcgtacttt acaaaagcaa tcttggatca cacaacaaat
     4321 aaaaaaacag cattgctttc tactatgaat acaacgttag atggcaaaac atatttcaaa
     4381 tcccatttga caacgcctga gtcattagat ttatatcgta tgatgtcaga agcagttgaa
     4441 aatggtatga ctcatctagt gatggaagtt tcttcacaag cttacaaaac acaacgcgta
     4501 tacggcttga ctttagatgt cggaatcttc ttgaatatct cgcctgacca tatcagtccg
     4561 atcgaacatc cgacatttga tgactatttc tattgcaagc gacaattgat cttgaattca
     4621 aaaacagttg ttttaaatca tgaaagtgac tattttgatt tacttaggga aacagccgat
     4681 ctattccaga ttccaacgat cacttatggt cgttcggaag aaagcaatta tcaagtgatc
     4741 cgttcagata aaggtactca cggttttact ttatcttctc atgaagatcg cttagctatc
     4801 tgcgatactg cttatgatat tttgctggct ggaggattca accaagaaaa tgcagccagt
     4861 gcgatcattg ccgcagcact aacaggtgcc tccagtcaag atgcacaaga cggattaaaa
     4921 gaagctcgtg taccaggccg aatggatcaa ttgatccaat ccaatggagc gcatgtctat
     4981 gtggactatg cacataatta tctaagcttg aaaacattgc tgaaatttgc caaaaatgaa
     5041 catccagatg gccgtgtaat tgttgtatta ggcagcccag gaaacaaagc aatctcccgt
     5101 cgacatgatt ttggaaaagt gctatcagaa acagctgaca tcgccttttt gacggcagat
     5161 gacccagcat ttgaagatcc gaaaaaaatc gcggaagaaa tcaatgaagc aattactaat
     5221 ccagatttga tcgttcatta tgaaatggat cgtcctgaag cgattcgacg cgctttagca
     5281 gaaagcgctc cgcaagattc agtcgttatt gcgggaaaag gcgtggatcc ctatcaaaaa
     5341 atcaatggtg tagatgaacc ttatgaaggg gattatgcaa tcgtgaagcg gttgattgaa
     5401 gaataatggt atttatatac tagtggtttc ccatttctaa gaaaaaaagg gactgtgatt
     5461 ttttgtcaca gttccttttt tcttagaaat cattttaact tttcactagc taattctgct
     5521 gccttttctc tttcttcatt taataattta ttatcataga atttgataaa tgggaaccag
     5581 ataatgaatg cgatgattgc acaaatgact gctaacacag cagctttcca atcaccacca
     5641 ctaccaacaa atgctccgat cccgactggt gaaggccacg gcatctgtgc aagcatcggt
     5701 ttgacgattt ccagccgaat ggcaaaataa gcgagtgtag cagaagccat cggagctaag
     5761 aaaaacggaa ccgctaaata gggattgtag acgattggca tcccgaaaat gattggttca
     5821 ttgatattga ataatcctgg tacaagtgag gcttttccta acactttcag ctgatcagat
     5881 ttcgcgagga atgcaataaa gatgatcaat cctaaagtcg cacctgatcc accaacggtt
     5941 acataagaat tgttgaattc tcctgccaac ggataatttg cgccttgtgc gttagctgcc
     6001 atattagcta atacgatcgg ggtcaagaaa gaagtgatga tattcgctcc atggatccct
     6061 acgatccaca atgcatggat caagaaataa atcaccatga caccaagcca tgtattcgtc
     6121 aactgcgtaa caaacccaaa tgggatagca atcattttga agatatccgt tcctagtgca
     6181 actaataatc cattgatgat catcaccaca aacgcaatga caaatgttgg gatcagagca
     6241 gtgaaagaac gagaaactcc tggcggaaca acatctggca tttttatgac ccaatttctc
     6301 ttgacacaca tacaatacaa ttcgaccgca ataatcgaca taataatagc tgtaaagatt
     6361 cctgaagttc ctaaacgttc gacaaaagag cccatcctaa taccatttac tacagtttct
     6421 gtctcattga tagaagcaat caagctgatt ttaccatcag tcatgatcaa ttcagggata
     6481 cacataaaga atgcaaaaac agatagtaat gcccccgtga gcggattaac atttaatttt
     6541 tgttcatctc gttcaattga cgttaattca taagcaaaca caatggcaaa gtaaatagat
     6601 aaaatcccca tcgtcattgt attgacgatc atgtacaagt cactaacttt gaaaaaagtt
     6661 gcattaaaaa atccttctaa aaatgggaac ggtaatggca aaatatttaa aaccaaaaac
     6721 atcgagccta caatcgtgaa aggaatagaa gccatccccg ctgccatgac agcccgaacg
     6781 attttaagtt gcgcaatttt ccccataggt cccatcaggt atttctgcaa aaaatcaaac
     6841 attagttatt ctcctttcct aaatataacg ctttttcata ttgtttaata cggatatact
     6901 gttggtcttg gttgagtgaa attttcttta aacgatactg tataaatgca ctggcaaaaa
     6961 taccgcttac aacaagaatg aaaacaaata actgtcctcc attgttattt gccataaaag
     7021 gatacaagaa tgtaaatcct gaggtaaaca acactggaat cactaataaa ttaaagacac
     7081 tctggaacaa aaaataccac tttgtccatg gaacgttcgt ttggtgtttt cgataaagct
     7141 ttacttgttc tcctactgca ggcaaaatag ctaaaatcag aatcatcggt aaactacttc
     7201 ctaaactttt agctgataga agcagaatca tccattgcat gttcatgaaa aagaaaaagg
     7261 cagtcaggta acgtacgatt agataacgat tgaaatacat gtttttcaaa ctaagttgct
     7321 ttttttctaa ttcgattttc ctttttactg tatcctgcac aatgatcctt cctttctatt
     7381 tatttttttg atacaaataa ttcatttcct gtgcaacttc caataatgtc atcgtcgtca
     7441 tcagatgatc ttgtgcatgg accatgataa tttccatttc tatttttgtc ccgctggcat
     7501 attctttcag aagttgagtt tgtgactgat gcgcatgaag aatctcttca tttgcttgtc
     7561 gcaacttctc ttctacttct tcaaacatct ctttacgcat cgagcgaaat gcttcatgta
     7621 tcagtgtccg tgcatttccg ctatggaaaa tgatatcaaa agaagtcact tgtagtgttt
     7681 cactgtttat tttttcagcc atctgttttc ctccttattc gatcagtttg ataaatttac
     7741 tgctgaaatt ctcaaaaata ggttcagcta agatttcctg ctgtactttt agctggtcaa
     7801 ctaacctaac gattgcttta gtcacgaccg taattccttc attttcgaca taagaaggcg
     7861 aaactaaaaa aacaaaatga atttcttgat attgatccca ctgcatccca gaaggaatca
     7921 atgcaacggc tacctttgtt tcgattccaa ctggaacagc gggatgtggg actgccactg
     7981 tatcactgaa tatgatttgt cccatatttt cacgttgctg gatctgcttt cgcatttctc
     8041 ttatatatcc ttcttcttca tttatgctca gcttttgcag caaatcatga agaatttctt
     8101 cctttttcgg ttcgtacgta taagtcttga aaaatttttc agacaactgt tcggtgtaaa
     8161 tctgcttttt ttgctgtaga gaaaatgctg gtatcggtct atctgaggaa tgatagggta
     8221 tagattctcc aactaccttt cgtattttac gaacatcatc ttcattcaaa aaaatactga
     8281 catgtaaaac tggaattttg aaaaacattg tcgaaagatc aatagaagaa ataatcaggt
     8341 cgattccttt taaagttgtt tcattgatct cataatagcc ttttacactt accactgtaa
     8401 tatttttacc gaattcactg actacgcgat tctttaatag ttgggcactg ccgtaacccg
     8461 ttgcacagat aatcaatgca tgtagtttat gcgcagctct ttctttttcc aatgccgcca
     8521 tgagatgcaa agccaaatag gcccattcat cttcattgat ggtatagttt ttcaaagccg
     8581 gcatgttgct gaaatgcctt tttactgcgt caaagacttt gggattttct ttctttatct
     8641 cctcagtcaa tgggttctca atgcgaatac ctcggctcaa tcgaatcatc atcggtgaca
     8701 aatgatcgag tagtccattg aaaagctgga gatcctctac taatgaaaaa tgaaattcct
     8761 gagacaatct tttcagaact ttcgctaatt cctttgatag ttcctgatca ttttgatttt
     8821 ctttgtgatt cgacttagcc atgagatgta atgtcaaata agcaatttct tcttcgggaa
     8881 acgatacacc tgcgattacc tcaatacgtt gaacgatccg ctgcgccact tgatactcta
     8941 ctcgcttaga aacttcttgt tcgatgccta attccttgat ttcaaaccct tctcggagcc
     9001 gtttgatccc caaagccaag tgtaaagtca gattctgtat gataaaatcc gaaactttta
     9061 acttagcttc tcttgtttca tcgagaatga taatgactag ctcttcaaaa ctgatgtctg
     9121 ggaaaaaacg gctttgccct aaatattctt tcaatgaatt tccataatgg tttccaaaaa
     9181 aagtatccat gataaaatgc cgtcgatttc tttcttcccc ttcgatccat gtcccttgac
     9241 catgcttaga aacgattttt agtgaatatg gaatcaatcg ctccttgatt tcttgcatgt
     9301 cctttgtcaa actggaacga ctgatataaa gctcttctgc caaatcatcc ataaaaagaa
     9361 tggcatcttc caaaagcaat tggttcaata tatacttttg tcgatcttcg gactcataaa
     9421 atccttgcgg ttctctagca tatccaggta ctatttcttg ccgactcaaa aacaagtcaa
     9481 atgtcagtct ttgtaaaata tgcagttggt agccataacc tggtttagca atgatcttac
     9541 ctccgttttt ttctactaac tctttgatcc gctgcaagta attacggatt gtccgatcag
     9601 ataataacaa ttcagaagct aaacgttgac tggtgacata ctccttcttg tgatcaatca
     9661 aatatctcaa cagcaatttt tccttctgtt tcattatcat ccactcctat tccatcatat
     9721 gttctcttcg atcacctctg ctaacttctg gatgcctgta gggattggga catacgcttg
     9781 gaaagggata ctaacaacag gctttccaac agattctcct aattttttga atttatccaa
     9841 aaacatcgtc gtttgcgggc tgatcagaaa taaatcaaaa tcactattct taatcatatt
     9901 atcgccttct gtagctgaaa ctgcatctac gagtacctct tctctttttt gcttaaagaa
     9961 atccgttgtc tttgaggcca tcatcgagga tgacattcct gccgcacaaa taatcaatgc
    10021 ttttttcata aatcaatcac tccttttatt tttataaacc aagcatatct tgtaagcgtt
    10081 ttctattcaa aatatatatt tccgtagcat tgcggaaaaa atgtgtatca tatcttgaat
    10141 aataatttct ggcaccgtga tatactagtg atagaatttt ttcaggaggg gatttagtga
    10201 gtatcacagt gaatattttg tatacaagta caaatggaca agccagaaaa tttgcagaag
    10261 aaatggtcga aagaaacatt gtcaaagcga ttcgtgcaga agaaggaaac gagaaatatg
    10321 actattactt tccactagaa gatccagaat ctcttttact gatcgaccgt tggaaagatg
    10381 aagaagcaat cgaaaagcat cacaaatccg aaatgatggc tcaaataaac gaactccgta
    10441 aaaagtataa attaaaaatg aaagtcgaac gtttcactga tttaccgtaa aaagatacac
    10501 atggaatgat agcaatttat catccatgtg tatcttcact ttttccttat tttttattcg
    10561 atctcatcta atgattcaaa ccgtgttttt ctcttatttt tcggataagt atttcagtca
    10621 ccccaatata catgattcct aaacataccc caaggatatt ctcaaaaatg cggatcgtta
    10681 ctgctcctgg aaccccgaat aaactggcag tgattgccaa agcaccaaaa gaattaaaaa
    10741 ctgttttata tctataggta gaacaaacac ctaaagctag accacctaat ataccgaccc
    10801 agttaaaagg aataaattgg ctgataccaa taaataaaac acatccaatc aatgagccaa
    10861 tgattcggtc tactgctcgt tctttgaaac caatcgacat caatccatag ctagatacaa
    10921 tagaagaaaa tgcaaaccct gcccacatca atcgctgaaa agggacatac tctcctgcaa
    10981 cgatcaaaag actaatgcca aatgcataga tgagcatcca aattttttcc tgcgaataca
    11041 tgcctttcaa aaaaatctct ttaaacaagc ttttgtcttt gttcttttct cgatgttttc
    11101 tgtaaaggag aactgaaaac caacaaaaaa acaaaaataa taagctactt gtttgtgtaa
    11161 agaaattctt gttcaacgaa tctttaggta acgaataaac aataaagaga tatgaaaagc
    11221 cgtacaaaga agcattcccc attttgggat cactagcggt tgccattaat atgctgctta
    11281 gtaaaataaa atgaatcaac atgcccataa ctgaccattt gatcagctgg acatacggtg
    11341 ctactaatag tgagaacatg acgattgcta aacctatgat cgaatgggaa atcttataac
    11401 caaagtcaac aaaacgaata ctcatcaaca tacagaataa aaccacagcg tatggtttat
    11461 gcttcgattc aaataatgtt gtaattataa tgataaaaca aatggcaaac aatacgatga
    11521 ataatgatcg taaaaacaat gaacaaatat acatgcactt ttctttttta gaagctgctt
    11581 ctcgtatttt ttgttttaaa ataaacggat ctagttgtag agactgataa aaatccacct
    11641 gttgtcgtcc tttcttttta cttttatttt ttacagtcat ctatgacagg ttgtaaatac
    11701 tatggaacat catatggcgt tgtcattttc ttgtcaacta tttttcctat tcatgttttt
    11761 ataaaaaaac atatcgtttt cttgtatgat taaatcacca caaaataaac aactgagaga
    11821 acgatatgct ttatgaatgg ttctatcaaa aaaattctga gatcaataga caaaaatttg
    11881 atgattacag aggtctctta cgaaacctta cagaagaaaa aaacattgat cgttgctgca
    11941 gttctcttcc ctgctccatg tgtctgtaga aattgtggtt ctgctgtggt agatggaaat
    12001 aggaaattaa tggtggtgaa aaacaagaag aaggaaacca ttgtccactt tgaataatat
    12061 aatcatatgc caatggttat acgcctaaaa aagcagcgct atacctgtaa aaactgtcga
    12121 agccattgga acgctcaaag ttattttatc cgtccaaggc actcgatttc aaatcatgtc
    12181 agacataaaa tcacttcttt actgactgaa aaagtgtcgt tattttttat ttcgaaaagt
    12241 tgttaggtat ctttgacaac cgttattcgc acattgaaag agttgagaag ctatttacca
    12301 aagtcttcca aaagaattct gtcttgagtg ttgagggttg tgaatttcgt tctcacgttt
    12361 ccgttgagga taaactgagt tttatttccg cggatggcga gacagaaaaa ttaatcgaga
    12421 aaatttacct acacaaaaaa ttcctcgatt aacaaactat ttccttagct gcactaatcc
    12481 taaagaaata gaattcttag ttacagacat gaacgccacc tactttcagc tcaccaaaca
    12541 tgttctacca aatgcgaaat tggtgattga tcgattactt ggtttttcca cgcctttcaa
    12601 ggaggcgtaa cctttattca cgagttggtt gaagctttcc gagacaaaag acccgacttg
    12661 tttttctcct tattgacaaa gagccattaa aactagagaa aaaattacaa ctgatcgctg
    12721 attatttgta gaaagaaaat caaagtatta cccaataaac ttgagtcatt aggcaaaaaa
    12781 cactgcgaaa ctcaagaatg attttcgcag tgtttttgta tattttttat taactagtat
    12841 tactacaaag gtttattttt cacttctcaa cgcagcacaa aattgtcttt acttatttac
    12901 tggttgggtt caaccttctc tactccgata ctaatatttg tttgtatacc ccttctaaaa
    12961 tcggtagttt ccattcaatt gatgggtact agttgatttt aaaaactatg cttattcaat
    13021 tttttatttt agctcatata tttcgtttct ttctaaattt tatactgatg aaaacaagta
    13081 gcacacccaa tattgacaat ataaactggc taatgatggt ttctccagtt tttggaagat
    13141 gcttatttcc actgcttgtt ggattgtttc ctcttatatc tgagctatca gcaacttttc
    13201 tattctcttt tgttaaaaag atcgccactt cttgttcttt ttcgatcttg aaagttactg
    13261 gtgtcgcatc caattcatac cccgttggcg cttgagtttc taccagctga tatgctcctg
    13321 gtgctaagcc atctatcgcc agttttccat ctgctcctgt ggttagacct gactgcagaa
    13381 cggttccatt ggcatcttgt aactcaaaaa cagctccttg caggacttct ccgctttggt
    13441 catcgatctt tgtcaacacg acaccgcctg gtgtaagtcg attttctttc gtcaattcta
    13501 ccaccgctgt ctgacttctt tcgatctcaa actcgattgg tgtcgcatcc aattcatacc
    13561 ccgttggcgc ttgggtttct accagctgat acactcctgg tgctaagccg tcaatcgcca
    13621 gttttccatc tgctcctgtg gttagacctg actgcagaac ggttccattg gcatcttgta
    13681 actcaaaaac agctccttgc aggacttccc cgctttggtc atcgatcttt gtcaacacga
    13741 caccgcctgg tgtaagtcgg ttttctttcg tcaattctac caccgctgtc tgacttcttt
    13801 cgatctcaaa ctcgatcggt gtcgcatcta attcataccc c
//
LOCUS       DARDHA010000066        13829 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000066 DARDHA010000000
VERSION     DARDHA010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.066"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            44..1468
                     /locus_tag="SDA10_001556"
     CDS             44..1468
                     /locus_tag="SDA10_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG4240469.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            1767..2474
                     /locus_tag="SDA10_001557"
     CDS             1767..2474
                     /locus_tag="SDA10_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEG4240470.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            2461..2784
                     /locus_tag="SDA10_001558"
     CDS             2461..2784
                     /locus_tag="SDA10_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEG4240471.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(2873..3910)
                     /locus_tag="SDA10_001559"
     CDS             complement(2873..3910)
                     /locus_tag="SDA10_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG4240472.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(4231..5214)
                     /locus_tag="SDA10_001560"
     CDS             complement(4231..5214)
                     /locus_tag="SDA10_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEG4240473.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(5629..6399)
                     /locus_tag="SDA10_001561"
     CDS             complement(5629..6399)
                     /locus_tag="SDA10_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4240474.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(6392..7327)
                     /locus_tag="SDA10_001562"
     CDS             complement(6392..7327)
                     /locus_tag="SDA10_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4240475.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(7643..9292)
                     /locus_tag="SDA10_001563"
     CDS             complement(7643..9292)
                     /locus_tag="SDA10_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEG4240476.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(9409..12012)
                     /locus_tag="SDA10_001564"
     CDS             complement(9409..12012)
                     /locus_tag="SDA10_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEG4240477.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(12024..13073)
                     /locus_tag="SDA10_001565"
     CDS             complement(12024..13073)
                     /locus_tag="SDA10_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEG4240478.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(13099..13413)
                     /locus_tag="SDA10_001566"
     CDS             complement(13099..13413)
                     /locus_tag="SDA10_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEG4240479.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(13429..>13829)
                     /locus_tag="SDA10_001567"
     CDS             complement(13429..>13829)
                     /locus_tag="SDA10_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4240480.1"
                     /translation="ASDKKSVVEKLSKLLETNGFVSDHKQFQKDVFDRENHMTTGIGK
                     NIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLLAIKDTDKGTNHLRILA
                     ELSGRLMDDEFVKRIKDSRNKKELVKALSF"
BASE COUNT         4068 a         2878 c         2376 g         4507 t
ORIGIN      
        1 taagcggtgt tatcattaag tggaaacagg aggtaaaaaa attatgacaa ctatgaaaaa
       61 gctaactttt ccagatgatt tttggtgggg ttccgcttgg tcagctgagc aagcagaagg
      121 aaaaggagaa acaggaaaag cagagactat ctgggatcgc tggtttaaag aacaacctta
      181 ccggttttat caacgaatcg attcctctat tacaaccgat cacatccatc aatataaaga
      241 agatatagaa ctaatgaaaa aaactgggca taactccttt cgcgtttcta tttcttgggc
      301 acgaatgttt ccaaatggcg gtaccggaaa agtaaatccc aaagccattt ctttctatcg
      361 aaaattattt tccgaaatga ataataacgg aatcaaagta tttgccaact tatatcattt
      421 tgacatgcct gctactcttc aagataaagg aggatgggaa tcacgtgaag tagttgatgc
      481 ttatgttcat tacgctgaaa cttgttttaa agaatttggt gacttagttt gtcattggtt
      541 taccttcaat gaaccacttg ggcctatttt aggtacttat cttgaagatt ttcattatcc
      601 aaacctcatt gactttaaac gcggagcgca agcagcattc ttcactatac ttgcacacgc
      661 aaaagcaata caagtattta aagaactagc tcttcctagt aaaataggcg ttattctaaa
      721 tttgagtcct acttttcctc gtagtcaaaa tcctgctgat gtgaaagcag cagaaatcgc
      781 tgatttattt tatacgcgga gctttttaga tcctatggtt aaagggaatt tttctaaaaa
      841 gcttatcctg ttattacaag aatataatca gcttcctgaa gattatacga aagaagatct
      901 tgtatgtatc tacgaaaata ccgctcaaat tcttggacta aactattacg agccacgtcg
      961 cgtcaaatct cgttcttcta tggtggatac atctagtcca tttttaccag agtggttttt
     1021 cgaaccacat atcatgcctg gacgacgaat gaacgaatac cggggatggg aaatttacga
     1081 aaaaggaatt tatgatttgt gcatggatat caagaaccat tatggaaaca tcgaagcatt
     1141 tatctcggag aacggaatgg gcgttgcgga tgaagaacgt ttcatggatg atacagggca
     1201 aataattgat gattatcgga ttgagtatat caaagatcat cttgcttact tatggaaggc
     1261 aataaatgaa ggatgtaaca ttaaggggta tcatctttgg acatttattg attgctggtc
     1321 atggatcaac gcctataaaa accgatatgg gctggtttca ctcgacctta ctactcaaaa
     1381 aagaacaatt aaaaaaagtg gcgaatttta caagcaatta agtgatacaa atggatttag
     1441 atacgatgta gacttgttag ttgaataata ttctaaaatt ccacttgaag tgaacacatt
     1501 agaaaacaag aataatgaga aataccaata acaaatactt tttcttcata cttcatttat
     1561 acttgtcagt ataagagact gtgacaaatg tagtgatcct cagaagttag gtctggaaat
     1621 ctaacttgag aagatttttt taaactacgt ccagtctttt ttatttgttt aaaattgaag
     1681 ttgacaagtc ttcattaaaa atttagaata ttcacaagtt tgacgacaat tatgaaaatt
     1741 tatgtcgtgc taaaggagtt ttgttattgg atgaaaagtt agatataaaa actgcgatca
     1801 aagacacgtt acctaccgtt tttggttata tcggtattgg acttgcattt ggtatcgttg
     1861 ggaaagctgc cgggtttcat ccgctggtcg tcacgttgat gtccctgctg atctatgctg
     1921 gttctgccca atttatcacg gtcagcatgc ttgctagcca tagtccactg ctctccatcg
     1981 ttctctcgac ctttctagtc aattcccgaa tgattctgat gagtatgacg attgctcctt
     2041 atttcaagaa aaatcggctg cttcaaaatc tgctgattgg cacattgctg acagatgaaa
     2101 gtttcgcact gggaatgaat aaattgaact atactggaca aaagttaaac tttcgttgga
     2161 tgaatacagc taattggatt tcttatttga cgtgggtggg ttcttctctt gttggcgcac
     2221 ttttagggaa ttttattact gatccgaaaa agtttggttt ggatttcgcc atcgtcgcta
     2281 tgtttatcgg gttactttat cttcaagtca tttctgatag aaacacctct aaacgattac
     2341 agttgatcct gattggtctc actttgattc tggtatatgt cggactgatt tttatcccaa
     2401 gtaacttagt gattttagtt gtaacactga ttgggtgcgg attgggggtg tggatcaaac
     2461 atgccttctt ttgagtttac attgctgact attttaggtt gtactttagc aacttggctt
     2521 tctaggatcc taccatttgt cttactgaaa aaattcgatt tgccgcaacc gttgcttgag
     2581 tatttaagtt ttgtcccgat cgtcattatg tctgctttat ggttcagcag tctatttacc
     2641 caaaatatcg gacatctgcc gcagatcaat ctcgaaaatg ctttggcttc agttcctaca
     2701 cttttagctg ctattctttc aaaaagctta ttggtgatcg tcttggtagg aattctttcc
     2761 ctttcgttga ttcgcttatt ataaataaaa aaacaagagc ttatggcaaa ttactgtcat
     2821 gcgctcttgt ttttatcaaa gtcaaataag aaaaaaatcg gcgtattctt gcttatccgt
     2881 tgaacggacc actaacaacg acttttccta atatatgtcc tgtttctacg tctttggttg
     2941 cttgtttcaa gttcttcgca ttcatcccat ctgaatagac cttgctcaat gtactgcgga
     3001 taatcccttc atttgctaaa gcagcaatgt gcgctaaagc ttctccttgc gtttcgattt
     3061 catagttata gtctgtttta gcgaacatat actcccagtc gaagctgata gattggtttt
     3121 tccaaacacc tatatctaac ggctctttga tccctacgat cgttcccaca tgtccaaatg
     3181 gtcggacaag gtgtttgatt tgatcaaagt agcgggtaat atcgaataat acagcgacat
     3241 actctacttg atcgattcct aattcacgaa aacttttact taagtcgtga tgataatcga
     3301 ttggatagtc tacacctgtt tttttcagcc attcaaaatt cttcggactt gcagtcgcgt
     3361 agacttctag gccagcccaa tgcgctagtt gattcaaaat cgaaccgacg cccccagctc
     3421 cgttgatcac taagattttt ttcccgcggt tggcattttc ttctggagtc agaccaaatt
     3481 tttcaaataa caattcataa gcggtcaatg aggtcaacgg caaggctgcg gcttcttcat
     3541 ctgaaagatt ttttggtgca agagcgacaa tacgttcgtc aaccagttgg tattcttgat
     3601 tgcttcctgc tcttgtcgtt gtacctgcgt aaaaaacgcg atcgccgaca gaaaattttt
     3661 gcacttcatc acctacagca acaactttcc cgactccatc aaaccctaag atacgtaaag
     3721 catttcttat cccgttatct tggcgaagtt tcgtatcgac aggattaaca gatactgcgt
     3781 tgacttttac cagaagatcc cgtgaactgg gtacaggtat ctgttctttt tcatcaataa
     3841 agctttttgg atcatcgatc gacaaccctt cataaaaacc gactaccttc attaattcct
     3901 tttctgacat acgaattcct ccttagcttc atcaagttgg agcatccata cagttttcat
     3961 ttcaatatat atagtatgct atttgttcca cttttattca tcataattat tctcttactt
     4021 aaagtaaact atttgcactt ttttggttaa aaaagaggtt gcgacaagac ttttgtcaca
     4081 acctctttta aacggtgttc aatcaactga ccctcggtat tcgctcaaaa aacgacaaaa
     4141 tatgaggagc tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcacg
     4201 acctctaatt cacggtgttc aagcaagaaa tcattgttca ctgaaaagcg ttggatgttc
     4261 tttttcaaag gtgtctaaga tctctcgtac ttcatcagta gatttcgtat tcatcaactg
     4321 aactcgcaag tcatttgctc ctggaaatcc tttgatatag attttaaaga aacgatgtag
     4381 tcctgtgatg gaacgcggga tgatttctga ataatgatct tgcagatcta gttgcagacg
     4441 aagtagtccg atcagttctt tttctgtatg ttccctcggt tctttttcaa atgcataagg
     4501 attcttgaaa atcccccggc caatcatgat gccatctacg ccatattttt cggccaattc
     4561 ttctcctgtc tggcgatctg ggatatctcc gttgatcgtg atcagtgttt gcggagcgat
     4621 ttggtcacgt aaagcaatga ttttagggat gacttcccaa tgtgcatcta ctttactcat
     4681 ctctttacgc gtacgcaaat ggacagaaag attggctata tcttgatgaa gtaggtgact
     4741 gatccattct tccatctcag acatttccgt atagccgata cgcgtcttga cgctgacagg
     4801 caaaccgcca gctttagccg cttcaatcaa ttgtgccgct acttctgggc gaaggatcaa
     4861 gccactgcct tttccacgtt ctgctacatt agggactggg cagcccatgt tgatatcgat
     4921 tccttggaag cccatttctg ccatagcaat gctcatttct ctaaaaaatt ctggcttgtc
     4981 tccccaaata tgggcaacca tcggctgttc atcttctgta aaggtcaatc ttcctcgtac
     5041 actgtctttt ccatctggat gacagaaact gtctgagttt gtaaactcag taaagaagac
     5101 atccggtgct gctgcatgct ttacgacatg gcgaaataca acatctgtta catcttccat
     5161 tggtgctaaa acaaaaaaag gctttggcag ctctttccaa aaattagtag tcatttattt
     5221 gttactctcc attctatcaa gcagtgaagt cgcaatcttt ctcaaaaaca ttgtcattat
     5281 actaggaaac aaaaaaagaa gcaaagtgaa cagcttaaaa gatgtttctg attgagcaaa
     5341 aaaagactgt gacaaaaatc ttatcacaat tccttttccg gaataaatgg tgttcaaaag
     5401 cttgcatctg cggtaataag tccaaccaaa cgaaaaatag gaggagctat ttttgtttgg
     5461 ctgttcttat tacttgatgc agaacagctt tttcacaacc tctccactaa acggtgttca
     5521 atcagctgac cctcggtatt agctcaacaa acgacaaaat atgaggaact attttcccgt
     5581 ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctcttttct aacttctcaa
     5641 tcctcttcct ttattgatca aataccaagt aacggcgtac aaaacaacga tgaatagcac
     5701 aagtacgatc atcgatgttg ctactggcac atcgattgtt ccaaggaatc cgtaacggaa
     5761 accggagatc atataaacga tcggattgac tttcgaaaca gcttgccaaa atggcggcaa
     5821 catagagatg gcatagaaaa cgccacctag ataagttaac ggctgcaaga caaatgtagg
     5881 cacgatcgat acatcgtcat aagactgagc aaaaatcccg ttgagcagtc ctgctaacga
     5941 aaacaagatt gccgtcatca ataatgtaat gatgaccatg aaccatgaat acacatgaag
     6001 tggtacaaag aacaaggaaa tgatcgttac gagagagccg acgagaatac tgcgacccac
     6061 tccgccaatc acaaagcccc aaatgatcac atgcgtagga acaggcgcta ccaagatttc
     6121 ttcgatgttt ttctggaatt tttgagagaa gaaggacgaa gaaacgttgg catacgaact
     6181 agtgatagca gacatcataa tcaaaccagg cacgatgaat tccatatatg aaaaaccacc
     6241 catgtcaccg atccgaccac cgatcatttt tccgaaaata acaaagtaca aggatgtcgt
     6301 gatcacaggc ggaaccagtg tttggaccca gatacgcaaa tagcggttcg tttccttgat
     6361 agctaaactt tttaatgcag taaaataaag attaaacatt tttctcctcc gtatgattat
     6421 tttcttctgt gattttcaaa aacagttctt ctaaacgatt cgacttattc ctcatagaaa
     6481 gaactttgat tccttgtctt gtcagctgat cgaagattcc gttcacccct tgatttcgtt
     6541 caacttcgac agcgattgtc tgttcatctt caaatgtaca agcgtatcct tcaatcacag
     6601 gtttggtctc atatggcgct aaatcaaaga taaatgtttc aaattgcagt ttggatagaa
     6661 gctctttcat actagtattc tcgatcagtt cacctgattg gatgatcccg atgttgcgac
     6721 aaagcatttc agcttcttct aaataatgcg tcgtcaaaat gatcgttgta ccattttcgt
     6781 tcaattctcg gagaaactcc cacatctcgc gtctcaattc aatatcgact cctgctgttg
     6841 gttcatccaa gatcagcaaa cgtggttcat gcatcaatgc cctagcaatc atcagtcttc
     6901 gcttcattcc cccggaaagc atacgtgcac ggacatttct cttttcccaa agattggact
     6961 gcttcaaata tttttcgctg cgtttcatcg cttctttgcg agagacaccg tagtagcctg
     7021 cttgattcac gacgatttgc tggaccgttt caaagggatt gaaattgaac tcttgcggga
     7081 ctaatccaat ctgttgcttt gctcggacta gatccgtatc cagatcataa ccgaagacat
     7141 tcacttttcc agatgtttta ttgactaaag aagtaatgat tccgatcgtt gtagacttcc
     7201 cagctccatt tggtcctaat agcgcataga aatctccttc tttcacggaa agatcgattc
     7261 ctcgcaatgc ttcgacccca cttccataaa cctttttcaa attatttatt tcaagcgcat
     7321 ttgccattta tttcctatct ccttatagac atgattcttt attgtatcat ggaatacagc
     7381 agatgaaaaa acaacttggt tccagcgaaa aatcaggttg ctgggctcag gaaaatctcc
     7441 taatgaaaga catcaatcgg acgaagtcag acaacctttt cagaaaaaac aaatcgaaga
     7501 taaaggatta ctattcatat gtgcaacaaa aggagcgtga gacaaaagta aagaacactt
     7561 ttgtctcacg ctctaaacac gtataaacgg cggaagcaaa agccaagatt tttgatccaa
     7621 taaatacttt cgatcacttc gcctatccca atacatgcca ctgccgcttt tcatcaaata
     7681 acgcggcttc cgtttttgcg gtttctacta aagtctctgg gtaatccaaa actttcagca
     7741 aggcaatcgc atttcgggtc cttgaaggtc cttcttttaa tttgaaatca aaactgattc
     7801 cttttccttc tgttacttgt tcttcaaagt ggacattctc acaataattc tttaaaatct
     7861 ctgtcaattc gatatcatgt gtggcgacaa atgctaaaga tggatactcc gcaagccacc
     7921 gaacgactga agcggaagaa gcaatccgtt cgatcgtatt tgtccctttt aaaatctcat
     7981 ctacgaagca gtagcagcgt tctttggaag ccacttggtc caacaaacgt ttgatcgatt
     8041 tgatttctga aacaaaataa ctgtcgcctt caaataaatc atcttcgatt gccatggaag
     8101 tcaacacatg tccaggttgc agggtaaact gttctgcaat cgctgtttgg atcgtctgtg
     8161 ctagaataca gctgatggct atacttttga catacgtaga ttttcctgat gcattggaac
     8221 ccgtgactaa tgtattcttc tgccagttga ccggattcac taccgcttct tttaacaatg
     8281 gatgatagat atctttcgct gtcacaccgc cctcagtaaa ctctggtcgg caagtgatgg
     8341 gcatataagt acggtaattc aatattgcag cggctacttc taattctccc agcaattccc
     8401 acagcttgat ggcttcagtt gaatgctttt ctaatttagt gagaacgaaa tgataggaga
     8461 taaatggcag catgaacatg atgttgacat attcaaaaat caactctgct tcactcccac
     8521 tcttcatacg aaatgaaaaa cctaatttag ggatgtctct taatggttta gtttgctgtt
     8581 tcagctcttc ttgaagtggt gtcggcagtt ttgctagctg attggcggct gaaatcgttg
     8641 aaaccaaata actcatgcta ttcagctctg tttctagtgt ttgtttcttc actagataat
     8701 agaccgtatt gaatactaac ccaccgattg ccaaatagat tcctagtact tgtcctaaaa
     8761 gaagcaaacc caaacccacc agcggaagca agccaagaaa aataaataaa cctacgcgtc
     8821 caatcttttt actttttcca tccgctagat attgttttgc aaaattatga tctttttttc
     8881 ctaaacgagc gaattgatat tggatttttt ctctcagttg aggattttcc tgataaaaag
     8941 caatcagttt ttctaactgc tgatcttcac caaaatcaaa gctgcgtaaa cgttgataca
     9001 acgcttctga acctacacta gaataggtac tgttgattcc ttcgaatact tcgaacatat
     9061 ccaggtcata ccaagtaaga tcatcgattt cgctatccca tgaacggaac ttcttctccg
     9121 tgagccatgc ttccttcaag ctttcttctt tatcaaatct cggttgataa ggaatttttt
     9181 cccatttgct acgaactgtt tcctttaatt tcaaacgtga aaaaatgtct aaagcaacta
     9241 ataaaataac agctgcaata ataattaaaa cgataaattc tctttgaccc atatccctct
     9301 ccgttccttt atttgttagc tttagtatag cactgttgcg ctatttgaaa agagggtaaa
     9361 aaaacacctt tcaaaataaa aggtgttttt ttcttgtata ttacatattt atctctcatt
     9421 tatcaaaatt gacaaataat catagggagg aattgttgtt aattcttgtt tattctccag
     9481 tgcattgaca actgtcccaa gggaaaggat agcctcaggt actatttttt cttgttctgt
     9541 tggattagct aagcgaagaa caaatccttg ttcatgaaaa taagaaggat aaatcgatga
     9601 tacaattaag tcttcaggta attcaatcaa tgaaaattta cgaggaacat caagttcatt
     9661 ttgtaatggc caaatcttgt tatctaacct gtgaataaat acattcagtt tctgcttttg
     9721 ataagaaata gattgttcta accgtttttc tgcctgtttt gccactttga actcattaaa
     9781 tcttccttct tctacattaa atccaaaagt gactttatac tcacctatct cttgagctag
     9841 cggtgtttcc atcatgacat gcccttcatt tgtcgtatct cctgaagctc gcccaggtcg
     9901 ccaagcaaga ttaggttttc caagttcccc tgttgttgaa aataaggtta tgattaattg
     9961 attttctata cgttcatact cttttaaacc atctgcataa aacgtaaaat aatgatcatt
    10021 ttctgccaca gagacactct tatcgaatat ttctaaatta acaggtttct ctaccatagt
    10081 ttctgaccaa ttcggaggaa tgacatcagg ggtattttca ataaatccgt tttgaatttg
    10141 tgcaattgat ttttcagatt tatctaaaac attaattgct aagcgcatac gatgcgagta
    10201 aacagtatta tgaattttga gtgtgccttc aacgatttga ctttctccca gactgaacga
    10261 tatttcataa gatatttctt ccatttcggg tttttcagat aaacgatctt ttaaatcttt
    10321 aggaagttga gcttttccgt aaacaactaa tgtttcttgt aaagaatctt tatatacttt
    10381 agctgtttct aattttagag atagttctgt atctccttct aatggtgaat aatcatacgt
    10441 atcgccatca tttgctgaat cgatcaaatg aacgaaatca agatattgtc tatctccaac
    10501 tatcaaattc aattgcccat ctttaaaaga aagtttatat ttattgttac ttatctcagt
    10561 tttgtctatc tcgattgctg tttctaattt ttcattattt ttctcaaatt caacaacagt
    10621 gtatcctagt gctggcacat ttacatttat ttttatgtca agttcataat aagctggttc
    10681 atcaaaatat tcgaaacctg tagcaatcat ccgataagca tttttccgtt ctggaaaata
    10741 aacttctttt tcaatgatat tatagggact acctttgaag tgtattttct ttgaatcagc
    10801 tactacatgc acgattttcg atccactata attctcagtc gttgtgttga aaacaaggac
    10861 ttcattcgat tttagcttta atgcatcagc catccgtttt acaataaggt tttcgattcc
    10921 gtcagctatc tcattagctt ctttgattcg atggaaaata tcttccgcaa cattatctga
    10981 aacacagcca ccaatactat catgagcctg gttttgcaaa atctttttcc ataaccgacg
    11041 aagtaggcct ttactgatat ggataccatt tctttctgca atcagcatga gcggctcaac
    11101 tcgttttaga attttttgtt ccaatttaaa attttctatt ttcatttttg ttcttactga
    11161 gccaatagaa cgatgaacac gagagtaaac cggctgacga agttcacctt tatattcagg
    11221 taattctttt ttgtttctga taacttctac ataagaagga taatctgaaa taatattgtg
    11281 gaatttactg tatttattca acttctctac gacttgatca aaatttaaaa tcattgactt
    11341 ttgatctaca cccgaaggta ctaaaatatc tttgtttttt ccttgcatat gaagcatttc
    11401 aactgcttca tccaactttt cttttgcaaa ttcaggaata tctttaattg cgtctaaggt
    11461 catcattcca gtcatataac caaatggaaa attcatggca taaacaaatt tatctcccaa
    11521 tcctttccag ataaaataag gagatggaac catcgtttca aagtttaatc ctcgccaaca
    11581 catgaaatta tcgatttcta cttgatgcaa aagtgtaggc aaattcgcat tgaacccaaa
    11641 cgtgtcaggc aaatacccta ccatcattgg atctccatat ttaaggatcg tatcactgat
    11701 tccaatcatt aagtttctta aaattgattc agcatccact aataaggcat ctgtttgtgc
    11761 ataccacggt cccacgaata gttgtcctgt tttcatcagt cgttggataa ccttaagctt
    11821 ttgcggattg atctccaaat actcatcgac gattgaagtt tgtccatcta aacaaaagtt
    11881 tactttaggg ttcctttcca attcatctag aacttcagta aagacctgat cagacaatac
    11941 gatggcatct tcatttgtaa aataccattc tctgtcccag tgcgtgtgtt gaattatatg
    12001 tgcatttacc atatcagatt ctcctacttt ccttcttcta ctgaatttac ttgttgttct
    12061 tttttatact tgatggattt tagtgcattg accattaacg ctgtaaccac tcccccaata
    12121 acaattgata ctaaatataa aggtatattc gtaacaaaag gaattgccat aaaggttgaa
    12181 agcattgttt cttgagtaac accaaaaaat ccatttagac cacatgctac tgcagtgccc
    12241 accacgatgc ttggtattac tcgtaaagga tctttcacgg caaacgggat cgtcccttca
    12301 gtaatcccca caaggcccat aatcatagct gtctttgcat tatctcgttc cgattcatca
    12361 taaagttttt tgtttaaaat tgttgctaaa aacattccaa atggcggaat agcaattcct
    12421 aataacatgg ctgtactagg tccaaagctt ccgcttgaaa aagaagcaac cccaaaagca
    12481 tatgcgattt tattgatagg tcctcccata tcaaacgcca tcattcctcc taaaatagcc
    12541 gacatcaaag cagcatttgc tcctgacatg ccttctagcc aattagtcag tcctgttgtc
    12601 attgcttgaa taggcgtagc tagtacatac cataatatga ttccaacaac taatgtagta
    12661 acaaaaggaa tcagcataat tgattttaga ctaataactt gcatcggtac ttttactttt
    12721 ttcaatgcat tagctaaata gccacaagct aatccagcaa ccattcctcc aataaatccc
    12781 ataccaagat tttgacacat catcccagtg ataaaagctg gtgctagacc gggacggtca
    12841 gcaattgaat atgcgatata ggctgaaatc atcggtacga taaactgtaa tcccgtctga
    12901 ccaatcattc gcaggactcc ccagaatgta tccgtctggt cccaaacacc tgtgccacca
    12961 aaaatgactg caatagaagt tagtaaccct gctgctacga caagcgggat catataggaa
    13021 accccacttt ggaaatgatt tagaatttct cttccttgtt tcttgaattc cattagtaac
    13081 tcccccacga tatatttttt atttgttaat taatgcaatt gcttcatcaa tcgtctcttc
    13141 accatctttt actacctctg aggtccctac tgttaaaatc ggtttaccgt caaagcgacc
    13201 tcgttctgaa acatttgtat ctactgcaaa tatgacaaca tctgcctcgg aaacatccga
    13261 aattgaaagt ttgttttcta tccccatagc cccctgtgtt tctactttaa tatctaaccc
    13321 tttcttcttc gcaaactttt ctaaattagc ttttgccata taagtatggg caatccctgc
    13381 gatacatgct gttactgcta ctatttttgt catatgaaat tcctcctttt aaaatgatag
    13441 agcttttacc aattcttttt tatttctcga atcttttatt cttttaacaa actcgtcatc
    13501 catcaatctt ccagataatt ctgctaaaat acgtaaatga ttagtacctt tatcagtgtc
    13561 ttttattgct aacaaaaaga cgagatcaac tggttgatca tctaatgcgc cccattcaac
    13621 agaccgcttt aacttggcta cagccactgt tgattcaatt actgctaggc ttttgccatg
    13681 aggaatggcg atatttttcc caattcctgt ggtcatatgg ttttctctgt caaatacgtc
    13741 tttttgaaat tgtttgtggt cagacacaaa cccattagtc tctaacaatt tcgataattt
    13801 ttcgactaca ctttttttat cagatgcat
//
LOCUS       DARDHA010000067        13810 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000067 DARDHA010000000
VERSION     DARDHA010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.067"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(221..1423)
                     /locus_tag="SDA10_001568"
     CDS             complement(221..1423)
                     /locus_tag="SDA10_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4240481.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="SDA10_001569"
     CDS             complement(1426..1620)
                     /locus_tag="SDA10_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG4240482.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="SDA10_001570"
     CDS             complement(1667..2515)
                     /locus_tag="SDA10_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HEG4240483.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="SDA10_001571"
     CDS             complement(2720..3070)
                     /locus_tag="SDA10_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240484.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="SDA10_001572"
     CDS             3665..4111
                     /locus_tag="SDA10_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEG4240485.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="SDA10_001573"
     CDS             4735..5727
                     /locus_tag="SDA10_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4240486.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="SDA10_001574"
     CDS             5743..6627
                     /locus_tag="SDA10_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4240487.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="SDA10_001575"
     CDS             6676..8283
                     /locus_tag="SDA10_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4240488.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="SDA10_001576"
     CDS             8284..9768
                     /locus_tag="SDA10_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEG4240489.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="SDA10_001577"
     CDS             9945..10937
                     /locus_tag="SDA10_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4240490.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="SDA10_001578"
     CDS             10989..11858
                     /locus_tag="SDA10_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG4240491.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="SDA10_001579"
     CDS             complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="SDA10_001579"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEG4240492.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT         4231 a         2337 c         2851 g         4391 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt
//
LOCUS       DARDHA010000068        13769 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000068 DARDHA010000000
VERSION     DARDHA010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.068"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            2..74
                     /locus_tag="SDA10_001580"
     tRNA            2..74
                     /locus_tag="SDA10_001580"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="SDA10_001581"
     tRNA            78..150
                     /locus_tag="SDA10_001581"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="SDA10_001582"
     tRNA            155..225
                     /locus_tag="SDA10_001582"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="SDA10_001583"
     tRNA            238..311
                     /locus_tag="SDA10_001583"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="SDA10_001584"
     tRNA            323..394
                     /locus_tag="SDA10_001584"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /locus_tag="SDA10_001585"
     CDS             660..1853
                     /locus_tag="SDA10_001585"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEG4240493.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="SDA10_001586"
     CDS             1983..3455
                     /locus_tag="SDA10_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4240494.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="SDA10_001587"
     CDS             3538..4326
                     /locus_tag="SDA10_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEG4240495.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="SDA10_001588"
     CDS             complement(4393..5061)
                     /locus_tag="SDA10_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEG4240496.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="SDA10_001589"
     CDS             5176..6888
                     /locus_tag="SDA10_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4240497.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8660
                     /locus_tag="SDA10_001590"
     CDS             6891..8660
                     /locus_tag="SDA10_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4240498.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8865..9431)
                     /locus_tag="SDA10_001591"
     CDS             complement(8865..9431)
                     /locus_tag="SDA10_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG4240499.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9431..10387)
                     /locus_tag="SDA10_001592"
     CDS             complement(9431..10387)
                     /locus_tag="SDA10_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEG4240500.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(10387..11043)
                     /locus_tag="SDA10_001593"
     CDS             complement(10387..11043)
                     /locus_tag="SDA10_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEG4240501.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            11413..13431
                     /locus_tag="SDA10_001594"
     CDS             11413..13431
                     /locus_tag="SDA10_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG4240502.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    13501..13707
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         4269 a         2489 c         2930 g         4081 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattatttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaactgt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcacgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaataa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
     8701 cagccaatca aaaacagctt ctcatgtttt tgatttggtt ggacttatta ccgcagatat
     8761 aagcttttga gcaccgtgaa taaagaggtt atgaaatcaa cgctctaacc tgagtattag
     8821 agaaaaaaac gcaaaacata tcatttatgt tttgcgtttt ttatttatct ttttttctca
     8881 atacaatata aaataggcgg ctggttcttt tggttgataa accgataatt cagcacatta
     8941 tatgtttcct gtggcagttt ggaacaaaaa gaatcaacca tatccagttc tttttctcca
     9001 ccttcatgac catagtagac gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat
     9061 atttcttcca tagctgtgcg tgtagtctct ggcatagtga tgattgcttt atcacttttt
     9121 gggagatacc ctagattgaa aatccctgct ttgagatttt gttgttcagc tatcacttct
     9181 cctaacgttt cgtggccttg tagaaacaat gagacacgtt cttctaactg acgctgttct
     9241 agcctttcac gagtagcatc gattgcttgt tgctggatat caaaactata gacatgacca
     9301 gtttttccga tgtgttctgc tagaaaaacc gtatcgtggc cattgcccat cgttgcatct
     9361 acgacgtgat cccctggttg caagatttct tgcagtaatg tatggctgaa atgcaaagca
     9421 gtttgcaaca tcagacgctc acctttttcc gtacatcata ttttccttgg aagctatctc
     9481 tgcgaagaag ttcttcatca attgcattca agacttccca ttttttcaag ctccacattg
     9541 gtccaatcaa ggaatcccac ggcgcatccc ctgtcaaacg atggatgatg atctcttttg
     9601 gtatcatttc cagctggtcg caaatgacat tgacatagtc aggacggctc attaattgaa
     9661 ggcgaccttc atgatagtcc cgcaacattc tcgttttgcg catcagatgc atcaaatgga
     9721 gcttgattcc ttggatatct gaatctaaaa tggtgcgtct gacattttct ttcatcattt
     9781 ctaaactttc gcctggtaat ccgttgatca aatgcgtaca aacacggata ttatgtttgc
     9841 gtaatttagc cacgccatct aaataagttt gataatcatg tgctcggttg atcagttttg
     9901 aagtttcttc aaaagttgtt tgcaatccaa gttctaccca taaatacatc cgctcgttca
     9961 gttcagcaag gtaatcaact acttcatctg gcagacaatc cggtcgtgta ccgactgata
    10021 atcctacgac acctggtaaa ttgactactt gctcaaatcg ttctttgatt atttcaactg
    10081 gcgcatgggt gtttgtaaaa ttttggaaat aaacgatgta ttgttggaca tgaggccatt
    10141 tttgatgcat catgtcgatt tctttctgga attggattgg aagagggtct tcaggtgcaa
    10201 cgatcatgtc tcccgaacca gatacactgc aaaaagtaca accgccatgg gcaacagtcc
    10261 catctcgatt cggacagtca aatcctccat cgatcggcac cttgaaaatt ttcccgccaa
    10321 actcatttcg taaagcgtaa ttccaagaat gatatctttt atttccttct gaataaggaa
    10381 aagtcattaa aagtccttct ttctgtaaga gttatctatt tgttttcttt tttctctata
    10441 cactggataa gtcaggcaca gccaagaaag actgagtaag taaccgccga tgatatcagt
    10501 aggaaaatga acgcccagat aaatgcggct tatcccgaca cttactataa taaacatcat
    10561 cagcaattga agcaatgtgc gtaaggttct tttttgaaca aaagctggaa gcagaaatag
    10621 taaagaaccg taaagaatca tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt
    10681 ttcaactact aagtgcgcca atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg
    10741 gttgatgatt ccagcaatta caataaatcc agaaacgaac caaaatgttt ctgccctttt
    10801 tttcttatac cacatccata aagccgcaca agcaaataaa atgatgacgg taagaggatt
    10861 accgaattta gtcataaaga gttggtagct gttcagatca ggataagaag ctctgacaat
    10921 gcctgtaaac cactgatcga aaccagaaag ccacgacaga tggaacttta cgatccagcc
    10981 caaaatgaaa aacaggacaa gaaagaagct accagccacc tgaaatgatg ttttatattt
    11041 catacaattc ctcctttgaa gcaatgactt attatagcat ttttggtcca gcgaaaaaaa
    11101 cggtaaagag ataaaaccaa tgtatgattc ctctttgaat aaccaatatt cttataaaaa
    11161 aaataactgt aatcggaaag acattttttg gtaatgcaaa tgaaatgtta gggaaataaa
    11221 atcagtgttt ttcgaaatcc ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg
    11281 aacagaagtt gttcattacg gtaaattaca atatgattac aaaaaacaac aaagctctta
    11341 aataactttt aataaatatt aagagaagtt ataattatta ataatagata aattgaagtt
    11401 ggaggataga agatggaaaa cattgcaaga aaagaacgga gacgtttaga acagaccaaa
    11461 cgttttcgaa aagcaaaacg aggggcagca atcgtgggaa ctgcgatggt aggctgttct
    11521 gtcgctgccc cattagtaca gccggttcct gttcaagcgg atgaaacacc tgttcagttc
    11581 ggcgcaagaa tcaatacagc ggcatttatc acagaaattg ctacttatgc tcaaccaatc
    11641 gctcaagcca acgatctata tgcatcagtg atgatcgcac aagcagttgt agaaagcggt
    11701 tggggcagta gtgcgttatc tcaagcacct tattataatc tttttgggat aaaaggcagc
    11761 tatcaaggac aaaccgtcta catggatacg cttgaatttt taaataatca atgggtaaat
    11821 aaaaaggagc ctttccgaca atatccttct tttgctgaat ctttcaatga caatgcctat
    11881 gtacttagaa atacttcatt tggcaacggt tattactatg ccggtacttg gaagagcaac
    11941 acgaaatcct atacagatgc gacagcttgt ttgacagggc gttatgcgac tgatcctggt
    12001 tacgcaggca aattaaataa tatcatcgca acttacggat tgacccaata tgatacacca
    12061 gctagcggaa acgcaggcgg tggagcgact acaggtggca ataatactgg aagtgcaggt
    12121 agtactggcg gttcaagcaa ttctggtaat acaaactcag gttcagcaac ttcgaccact
    12181 tatactgtcc aatctggtga ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg
    12241 gatcaattga tccaatggaa caacatccaa aacaatttca tttatccagg acaaaaactg
    12301 acaatcaaag gtagccagga aaatggctct tcgacgaata attccggtaa caataccaat
    12361 tcttcaggaa atgctggtac atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt
    12421 caatctggtg attctgtttg gaagatctca aatgatcacg gaattacgat ggctcaattg
    12481 attgaatgga acaatatcaa gaacaatttc gtttatccag gacaacagct aatcgtatca
    12541 aatggcggtt cttctaatgc tggagcaaat actggctcaa atactgggaa tacaagtggc
    12601 tctagttcgt ccagctcaag ttcttctaat acagcttcag gaacgaaata tacagttaaa
    12661 gccggagact ctgtttggag cgtctcaaat aaatacggaa ttacgatggc tcagctaatc
    12721 caatggaaca atatccaaaa taactttatc tacccaggac aacaactgac tgtttcaaac
    12781 agtggatcaa caaacagcac gtcaggtaat acaggttcgt caaccaatac aaataacagc
    12841 agttcagtta atactggcag cagttcgtct caaggcactt ataccgtgaa agcaggtgaa
    12901 tctgtttgga gcgtggcaaa taaaaacggc atcacgatgg atcagttaat cgaatggaat
    12961 aatatcaaaa acaacttcat ttatccagga cagcaattga tcgttaaagg tggaacttcg
    13021 gcaaatacaa atacaggttc aactacttct gcagcgaaac caaatacacc gaatacgtct
    13081 gctacgacaa gtacatcatc cactggtaat acgatgtata ccgtgaaagc gggcgagtca
    13141 gtttggagcg tagcgaacaa acatcatatc acgatggatc agttaatcga atggaataat
    13201 atcaaaaata atttcatcta tccaggacag caagtgattg tgaagaaagg ttcatctcaa
    13261 tcaacaaacc aacaatcagt tactggttcg aaaacctata ctgtgaaatc tggagaatct
    13321 gtttggagtg ttgctgacag tcatggaatt acaatggctc aactaatcga atggaacaat
    13381 attaagaaca actttattta tccaggtcaa acgttgattg tagctaaata aaatagctgt
    13441 tcgaagaaat aacgattgaa ttccacaaaa atatttgtta ctataaggct acttaaatat
    13501 gacaacactt gaaaaggaag tagtaaaaag actgaagttt cagagagcgc atggctggtg
    13561 agaatgtgca cggatgcttt tgaactcgcc tttgagttgc ccttgctgaa tacccgagta
    13621 agcaagcggc ggtgacggcc gttacaacgt tcgaggcctt ttcactaagg taaaattagg
    13681 tggtaccgcg tagattacgt cctataaaaa gagcaatctt tttataggat ttttttattt
    13741 cttttgtgaa gtttgagaaa acaaagcaa
//
LOCUS       DARDHA010000069        13613 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000069 DARDHA010000000
VERSION     DARDHA010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.069"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(67..498)
                     /locus_tag="SDA10_001595"
     CDS             complement(67..498)
                     /locus_tag="SDA10_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEG4240503.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            577..990
                     /locus_tag="SDA10_001596"
     CDS             577..990
                     /locus_tag="SDA10_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEG4240504.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(1028..2176)
                     /locus_tag="SDA10_001597"
     CDS             complement(1028..2176)
                     /locus_tag="SDA10_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG4240505.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(2298..2894)
                     /locus_tag="SDA10_001598"
     CDS             complement(2298..2894)
                     /locus_tag="SDA10_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG4240506.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(3094..5736)
                     /gene="alaS"
                     /locus_tag="SDA10_001599"
     CDS             complement(3094..5736)
                     /gene="alaS"
                     /locus_tag="SDA10_001599"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEG4240507.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            6124..6555
                     /locus_tag="SDA10_001600"
     CDS             6124..6555
                     /locus_tag="SDA10_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4240508.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(6600..7949)
                     /locus_tag="SDA10_001601"
     CDS             complement(6600..7949)
                     /locus_tag="SDA10_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG4240509.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(8019..9182)
                     /locus_tag="SDA10_001602"
     CDS             complement(8019..9182)
                     /locus_tag="SDA10_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG4240510.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            9311..9634
                     /locus_tag="SDA10_001603"
     CDS             9311..9634
                     /locus_tag="SDA10_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240511.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(9817..11166)
                     /locus_tag="SDA10_001604"
     CDS             complement(9817..11166)
                     /locus_tag="SDA10_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4240512.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(11235..12188)
                     /locus_tag="SDA10_001605"
     CDS             complement(11235..12188)
                     /locus_tag="SDA10_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEG4240513.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(12290..13480)
                     /locus_tag="SDA10_001606"
     CDS             complement(12290..13480)
                     /locus_tag="SDA10_001606"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEG4240514.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         3956 a         2856 c         2353 g         4448 t
ORIGIN      
        1 gaaccgtacg tctcactctt ttcttttcct gtaaaaattc gtttttttag gttctgattt
       61 ttaaaattag ctttttcttt ttttcgtata aggataagga attgattctg atttgggtcc
      121 gtttttttca aaatgatcaa aaaggactac ttgatctttt tttcgcatca tgaaaacaga
      181 tggggctttc tcatcttgca acatcatttg caccccagta ttagttggcg tcaaagatcg
      241 aatctgtccg acagatattt ctcggtcttt ccaaaaagca gcataacgta taatcacaga
      301 gtttgatttt aaaataaaag aacgccgcaa acctagaaat agaaaaacaa gaaaaatacc
      361 taaaacaaaa ttgcttttcc aataaggacc tgtgttttct aaagaaagaa tcagactaaa
      421 aaacagaatg atcaaggtca atgaccaata gatgatcgta tgtgccagtt caggctgcca
      481 tcgaatattt tttttcattc tctaacctct caccgattca gtaatgatat aatcttacca
      541 taacattagt ttgaacaaaa aggagaatct ctttttatgt taaaagtata cattgatgcc
      601 tctacaaaag gcaaccctgg tcctagtgga ggtggaatct tgattgtcta tcaaggtaaa
      661 caagaacagc tatctatccc gctttctata gggacaaacc atcaagcaga gttcgaagtc
      721 tttttaaaaa ctttagaatt tctaaagaaa aacgacttac agaatgaaac gattttctgt
      781 ttttcagaca gcaaaacact agtttctacg gtcgacaaaa acgcaacgaa gaacgagctc
      841 ttccttccct accttcaaag aatccaagaa cttctatccg aattttcttt attgatcctg
      901 caatggttgc ccgaaaacaa aaataaagga gcagataatc ttgcacgtca ggctctccat
      961 aaacaattaa aagaaaaaaa gactctttaa attaaaaact ttaaggagtc tttttatagt
     1021 ttctcattta atataaaaga taattgtgat caaacgtaat ttccaattgg taatctccga
     1081 ttttttcttc tatttgacta cgtaagattc tttctagttc tgtatcaaca aactggaatt
     1141 tctcagggac cactacatca aattgcagcg tttttccttg atgaattact ctaaaatcat
     1201 gaatattaag ccctgtacct gtttcaagaa gaatttgctt taactgccgc aagatattat
     1261 agtatttttc gttatcaaca ggcattggat ccaagtgaca gacaagttcc acatccaaag
     1321 actttttaaa gtctttttct atcagatcaa taatcttatg agccacattc aaattcattg
     1381 attcatctac ttcgatgtgg actgatgcaa agcgtttatt gggtccatag ttatgcacca
     1441 ataaatcatg atagccaagt atatcatcgc aagcatttaa atgctcttcc atttcttcaa
     1501 tctctgctgg cgttgggcga cttcctaata gttcgtatac aaaatttcga agcatcgtca
     1561 atccactata aaggatatat aatgctaaag caaaacctat atatccatca atacgccatc
     1621 cagttaccca ttcgattgct gctgaaagca atacagccag tgtagtaaag acatcattat
     1681 aactatctgt tgctgtagct ctcaatgttt cagaatcaat ggattgcgca ataacctgat
     1741 acattttgcc ttgtgcaaat ttcaaaaaga tggaaatgat taggacaaaa aagacgatgg
     1801 gtgttaaaga gacattttct ggatggatga ttttttcagc agaagactct aaaaattgaa
     1861 atcctacata agtaatgatg atcgaaacaa ataacccact gatatattcg aagcgttcgt
     1921 gtccataagg atgttcctga tctgctggct ttgcagcaat tttaaatccg atcaaagtca
     1981 atatggatga agcagtatct gatagactgt tcactgcatc agccataatg gagacacttc
     2041 ctgaaaaaag cccgatcatt agcttagaga caaacaagat cagattcgta accaatccta
     2101 agatacccgc tagtactcct ttttttgtcc gctgttgcgg tatttgtttc tttttttctt
     2161 cttgtttctt agccaagttt tttacccctt ctagtttgct tgttgctcag gtaaaaagat
     2221 ggagaggacg attgttttcc tctcctctct tcctttttct atctatcgtg cgtcctatta
     2281 ctattaaaag atggtcatta ttctggtttc aacggtgttg ctgcaaataa cgcttttgct
     2341 agtaatggtg ttatgattcc cgcacctatt gcttctggta tcccattgat tgctgcaacc
     2401 cctgctaagg ttttaaataa aagagcagag tctacaccat aggcatctgc cgttgcacct
     2461 gtataaaata atcccatgaa tcctaacact aagactgtat tgaccaatga acccagtacc
     2521 cctgcaaagg ctgagctgat cgttattgat ttattttttt gataaatcca gtggaaaacc
     2581 aagcctgcta ctaagccaac aagtactctg ggaactaccg agataatcgg attcgtgaac
     2641 actaatgtat caaatggcgt tgttggcatg gcataggcac gaacaattgt agcgattccc
     2701 caaaacaagc caatcaccat gccatctttt gggcctaata ctactgcagc aataattact
     2761 gtgatatgga taattgttaa actaatgaat cctaatggaa taaaccccag ccaaggaacc
     2821 atcgcttgta cgatgatgat tgctaacaag atcgcacgta agactagtcg aaaggtctta
     2881 tttctactat tcattttttc accccttttt atttatacta atcgtaaaaa tttctgtcac
     2941 tttcgtcaac taaagaccat ccgaatccga ccatttgatt agatgcgcca tccaattgaa
     3001 aaaagtatga gggcaagact tgtttgtctt cgcccccata atcatgtttc gttcaatgtt
     3061 tgaacttttg tttttctgtc aaacgtaaac gccctattgt tgcgcaaccc attctttcgc
     3121 agctttaagt gcatctggaa taccagcagg atttttcccg ccagcttgtg ccatgtctgg
     3181 acgtccacca ccgccaccgc ctactttcgg tgcgattgct ttaatcaagt cgccagcttt
     3241 tagtccagct tcatttgatt ctttcgtcat tgccgctagc agactgactt ttccttcttg
     3301 ttcgtttgct aaaacaagaa tatctgaaat atttttttgt ttccattggt cagctaattg
     3361 acgcagctga ttcatatctt tcacttttac ttgtgctgca atgattgttt ttccattgat
     3421 cgtttctgga tctttgaata catcaccagc ttgttgattc gctaacttgc tagctaattg
     3481 ttcattttcc ttttgtagct ctcttacttg ctgttgcaat tgttccgctt ttgaaacaac
     3541 ttctttcaat tgaggtgatt taacaattgt tgctacttgt tttagttgtt tttcttcttc
     3601 ttcaagtagt tcgtacgctt ctttacttgt tacagcctcg attcgtcgca cgcctgcacc
     3661 aattcctgat tcagaaacga ttttgaagat tccgatgttt tctgtatttg ctacatgtgt
     3721 gcctccgcac agttcgattg accagccgcc aatatttact actcgaacgt catgtccgta
     3781 tttttcacca aacaaagcca ttgcacccat gtttttggct gtatcgatat ctgtttcaat
     3841 tgtttcaact gggatagctt cccagatttt ttcgttgacg attctttcca tttcagctaa
     3901 ttcatctgaa gtgatttgac caaagtgggt aaagtcgaat cgtaaatgtc caggagcgac
     3961 taaagaacca gcttgatttg catgctcgcc aagtacttct ttcaatgcct tgtgcagtag
     4021 atgggttgct gtatggtttt tgatgatccg attgcgcatc gattgatcga tcaatagatg
     4081 ataggtttgt ccttcactca ttggtgcttt tacttctacg gtgtgcataa attgaccatt
     4141 aggcgctttt tgaacatctg taacaactgc tacgatttcg cctacttcat tcatgatcca
     4201 tccttgatcc gctacttgcc cacccatttc tgcatagaaa ggtgtttgat caaagatcat
     4261 ttgagcttca ccttctgcga cttcattcaa aagagactca cctttaacga tgaccaataa
     4321 ttgactatct gattcttgtt ctgtgtatcc aatgaattta ctttctactt tgatatctgt
     4381 caataaagca gattgtaccc ccattgattt ttcagtactg cgggcagagc gagcacgttc
     4441 gcgttgtgca tccatttctt tttcaaaacc agcatgatca actttcagtc cttcatcttc
     4501 tgccacctct tccgttaatt ctaccgggaa gccatatgtg tcgtatagtt tgaagatgtc
     4561 ttttcctgct agagtcgtcc cgttttcgtt ttttacttct tggatcactt gatttaagat
     4621 ttccaaacct tcattgattg tttcattgaa acgttcttct tctgtacgga taactttttc
     4681 aataaattct ttttgttgaa gtacttcagg ataataactt accatgattt ccccaacaac
     4741 agggactaaa cgatataaga atgcttgttc aatacctaat tttttcccat gcatgacagc
     4801 acgacgaagc aaacggcgta agacataccc tctcccttca ttcgatggca atgcaccgtc
     4861 gccaatagca aatgaaaggg cgcgaatatg atctgcgatt actttgtaag aaatatctgt
     4921 tactgggttt tcaccatatt ttacttgttt gcttagcttt tctacggcgt ggataatagg
     4981 cataaacaag tctgtctcaa agttcgttga cgcatcttga acgattgaga ccatgcgttc
     5041 caatcccatc cccgtatcaa tgtttttatg aggaagtggt tcaaatgtgt tatcaggctt
     5101 atggttgaat tctgaaaata ctaagttcca gatttctaaa taacgttcat tttctcctcc
     5161 tggatagttt tcaggatcgt cttctgctaa atcgttataa gcttctccac ggtcatagaa
     5221 aatttccgta tccggaccac atgggcctgc accgatatcc cagaagttgt cttctacatc
     5281 aataatgtga tctagtggca ggccaacttc ttcatgccag atgcgttttg cttctgtatc
     5341 ttttggataa actgttacat aaagtttttc tggatcgaat gccatccatt ttggatcagt
     5401 caaaaattcc catgcccagt ggatggcttc atttttgaaa tagtcaccaa tcgaaaaatt
     5461 acccagcatc tcaaacatcg tatggtgccg tgctgtttta ccgacatttt cgatatcatt
     5521 tgttcgaata gatttttggg catttgtgat tcttggattt tctggcacaa ctgaaccatc
     5581 aaaatatttt ttcaatgttg caacaccaga gttgatccac aataaggttg gatcgttcac
     5641 ggggatcaaa gacgcactag gttcaatcgt atgtccttta gattggaaga aatcaagaaa
     5701 catttgtcga acttgtgcac tacttaattg tttcatgtca ttacctgctt tctataaatt
     5761 aaatacaaaa aaacaccatc acagccaagg acgaaaactc gcggtaccac cttgattgca
     5821 atgatgtgta atcattacct cttaaactcg ataacgctga gctgcgtcgg tatttctacc
     5881 aaaatagaaa aagggagcaa tttgattagt gactattctt ttttcagcca agaaagaatt
     5941 ttctgtgagt cttaaaaaac aaatcatatc cttttcgtac ttaattagta tacgcattct
     6001 attgatcact gtcaatatct caatttttat tttcatttcc ttttggtcat actccacgat
     6061 ttttggtacg ataagaataa aggaatcaat acatctctgc tattcatgac aaggaggaat
     6121 atgatgaaaa aaatagttgg agcggatgaa tggatacgcg cagctagctg ctttttgaga
     6181 tatcaagttt ttgtattaga acaagggatt cctgcagaac tagaattcga cggaaaagat
     6241 atcccgcaac aaacctatta tctatttctt gatcaaaaaa gaccagtagc tactttgcgt
     6301 tatgaaaaga aaacacctac ttgcttcaag ccggaacgat tttgcgtagc aaaagaatac
     6361 cgaggaaaag ggttaggcgg tcagttgctt gaagctgctg aaaaacgggc ggtttcagaa
     6421 ggttgtcaga ctagttatgt agtagctgaa atgacggctg tagattttta tgaaaaacaa
     6481 gggtatcata cttgcacaga accttttatt gaagatgaag tactttgtat cggaatgaaa
     6541 aaaagtctag cttaaaaaca agagattgtg acacgccttt tgtcacaacc tcttgttttt
     6601 tatttatgtg aatttttctt atttttacga gcagtacggc tttgctgtct gcgttcgatt
     6661 ttgcgttctt ttttattttt ttctgcaatt gcccaatcga tttttttctt ataaccaggc
     6721 ttgatctttt tctttttctt cttcacaaga ccaatcagcg tgggatctaa tacatctttt
     6781 gatttttctc gtttggcacg tctattacga tcgtatgttt tgacgatttc cccgtttttg
     6841 atttcttttg gttcaaatga gacgcccaat ttttctatcg catcaatggc ttgctcatca
     6901 tttggcgaat ataacgtaat ggccgttcct tttaagccat tacgtccagt acgtccgaca
     6961 cgatggataa agaagtctag atcttctgga atttctgcat taataacatg agagacacct
     7021 tcgatatcga tccctctagc agctaaatca gtcgctacaa catattgata ctccagattc
     7081 tgtacttgtc gcatcactcg ttttctctct cttggggtga tgtctccatg aatctttgct
     7141 actttcaagc cttggttttt caagtaatcg gcaatttcat ccacatgctg ttttgtattg
     7201 gcaaatacga ttgctaaata aggatgacca attgtcagca gttgataaat catttgattc
     7261 tcatctttac cttttgttga aatcagccaa ttttcaatgt cttcagaaat aactgctttt
     7321 ggtttgattt cttcaatgat tggattttcc atgtattttc gtaagaatgg acgaagtttt
     7381 tctggaatag tggcagaaaa aactaaaaac tgcagctttt ctggtaatcg acttgcaatt
     7441 tggtctactt catttaaaaa acccatatcc aatgtcatat ccgcttcatc gacaacaaat
     7501 gcaaatgcgg tgtgaatctt cagcgcttgt tcattcatca tgtctaatat tctaccaggc
     7561 gtgcctacta caacctgtgg ctgttggtga tgcagacgat ttaattgacg ctgtttgtct
     7621 gtaccgccga caaaattcga gacacggatc tccggtgtag aaaatttagc gatttgtgta
     7681 gctgcttcat agatctgtgc agctagttcc ctacttggag ctgtaataac aacttgtact
     7741 tcctcttttt ctgcattgat tttttcaaac aaaggcaaca aaaacgtatg tgtttttcct
     7801 gtaccagttt gagattgccc aatgatattt tttccttttg caattaaagg aatcaaacgt
     7861 tcctggacct ctgtgggctg tttgaagccc tttgcggtta acgcttcatt gataaacggt
     7921 ttgaagcgga atatatcaaa attattcatt cgtgcacctc ttctctttaa attatcacga
     7981 ctttggcatt ataacacaga caaagcgttt ttgctcagct aatttcgaga atcatcatcc
     8041 tttgtttggc caatcgtgct aacagtatat gtgtgactag ctttttcata aaacagaatc
     8101 cgtttttttc tcaaaaatga tagcaagata tttaaaataa aaataccttg tagtaacaag
     8161 acaccgagag aaatgaatac tgcactcatc tgcaacaccg tattcgttgt cgaattcatg
     8221 atttgctgaa gcaactgccc caaccagctt aaaccataaa aaagaaagta cagatacccg
     8281 tagcggatca tcaatgcttt taaagaaatt cgttcgcggt cttcttccac caaacggata
     8341 cgcacaattt ttttgccaag tgtccatcca ttggttataa acggtacaat cataaagtat
     8401 acccctactt ctaataaaag ccaccagata tgagaagtaa ctgcccctga atcgaacgca
     8461 attttaaaga aaatcgccgc taccaaatta ataagagata aaaatgtcca gtcgatcaaa
     8521 taagcgacca agcgtcgagt caatgagaca gtctttcctt tttcataaga aagttcatcc
     8581 attcgtcttc ttgtaggaag cataaatgta aataacggcg taacgacaaa accaatgatt
     8641 ccacccaatg tatttgtcac taaatcattc acatcaaata agcggtatgg tcttgggtag
     8701 ataaaataaa gtccacttag ctgcgtcaat tcaaaaaata gagaaaggca gaaactagca
     8761 atcatcgttt tcataaaaga aagccggaaa taatatctta aataaatgcc aaatggtatg
     8821 agtaataaaa cattaaaagc tggttccaaa aatacactct gcttcattgc cggcaggtag
     8881 gtactaggtt cagacaagac caataccgtg ttgtctctga aatgttgcca tgaagcaaat
     8941 aaatgcagct ccatctttgg ccctgtcatt tgggcaaccg tctcctttgc cggaagtggt
     9001 aagatcacca aaaaatacgc acacaataaa taaaaaacga aagaatacaa aataactgcc
     9061 cttgttacta gaaatgttcc atactttcga tattcataaa taaaaaaaac agatgagatc
     9121 gctaaggcta aaaatggaaa aacaatcagc gcaaaacgaa tcggttcgat ataacttgcc
     9181 atatgactcc tccttgattc tttgtatctc tcttatctta gcagtccttt gctgtttcaa
     9241 gcaagtaacg aggctattat ttctcatttc tttcaaagta aagtaagata aacacaaagg
     9301 gggtgtcgag atgctgaata tgtactttgt ttttggcgta cctatttttt tactgttcct
     9361 ttatgctacg atcgcttatg taagaaaaag aactacaatt cattatttag gatttattct
     9421 cctgattatt tcgggtttca tgctagtctt caatcttcag acttggcagc aagcacttct
     9481 tgaaatggat aaaatgactc cacatgcttt gtctaaagtg cttggctacc ctgtctatct
     9541 gatttggctt cccattttca tttctggttg cttggtttta ttaaatattt atcgcggcgt
     9601 acgccgaata gttcagttac gcaaatcaaa gtaaaagaca gaaatcggtt gagcccgatt
     9661 tctgtctttt actttgctat ttcatcgtag acaaaacgac atttacgtga tcaaaattaa
     9721 cgagtacttt tgacatttct tccacgtact cctcatttaa ataaaatatt ttgtttggca
     9781 gagaagtgca gttctaataa aattcgaatg ttttagctat ctacgtttta aatagagata
     9841 aatcgggata ccagcaagtg tcaatactat ccccatcgaa gctaaaaagg tctgagtgat
     9901 aagcgtgctt gatacgataa aagtccctcc aatgattgct actagtggaa taaacggata
     9961 cattggaact ttgtatggtc gaaacaaatc aggttctttt ttacgaagaa gaatgacacc
    10021 aacaaagacc atcgtataaa aaatccaaat gacaaagatc agcatatccg ttagtgtatc
    10081 aaaaccaccg atcatcatca tgccaatagc gatgactagc tctaagatac ctgcggctac
    10141 aggaacttta gttttatcat gaagtttagc aaagagcttg ctaaaaggca ggttgttttc
    10201 ttttgccata acataaggga gacgcattcc agtaagggta tagccattga tggttccata
    10261 aacagaaatc aagataccaa ctgtgaccag tcttccaccg aagccgccaa aaatcatttt
    10321 tgcaacgtca cttgccgcat tgctgtttcc tgcgactccg tcgatagatg cggttcttaa
    10381 gaacaccgca tttactaata aataaacgat catgattccg atgattccta aagaaattgc
    10441 ttttggcaaa tctttcgccg gctttttcaa ttctcccgaa atatttccta cgtgtatcca
    10501 accgtcgtat gcgaacatcg ttgctagcaa gcccgctccc aaggctgaga aaaatgaaag
    10561 attttctcct gcttggatcg gaaacagttg gaagtctact ccttcttgtc ggaacaaacc
    10621 gaaaatgacg atcacaaaca aaggaataag cttgcagaca agcgtaatcg actgaaaagc
    10681 ccctccagct ttagaaccta aaaaattgat caacaggatc gatacagcag ctgtaacagc
    10741 tacgggaaca atcattgact gggaaagacc aaatagattg acgaactgtg taccgaaaat
    10801 gatcgacaag gctgctacat ttgctggaaa ataaatcacg acttgcgccc agcccaataa
    10861 aaaagctgct gtatttccgt aaatccgttc gatatattta atcatccctc ctgtttctgg
    10921 aatagcagct gccagttcag ctcctgttag tcctgcacag acagaaatca taccaccgag
    10981 aaaccaagaa aacatatgca aactggcgga tcctgtaact tctgctacgc ttgcggcttt
    11041 aaaaaatacc cctgcaccga tcactgtgcc cataacagtc gataaagcag taaaaaatcc
    11101 catcgtacgt tttaattcat tttctatttt tacttctttt actggcgtgg cttgatattc
    11161 ttccatccct tgtccccttt cataccatag ccaatcccaa aagtataaaa aactttttgg
    11221 atgtggttct tgttttatac cgtctctacc aatttttctt tcagtttttc caacatatcc
    11281 tttgtcatca catctaagtc gtatttcggc gaaaagcccc attcctcttg cgcacaagaa
    11341 acatctagtg aatttggcca agattctgct attgactgac gtacaggatc cacatcataa
    11401 tccatttcaa aatctggtat gattttttga atagatgctt tgatctcttc cgggtcaaaa
    11461 gacatcgccg atatattgaa ggcatttcga tgaaccaatc gatcaggatc tgcattcatg
    11521 agttttataa tggcatcgat cgcatctggc atatacatca tatccattga tgtaccttga
    11581 tcaatgaaac tagtgtactt tccttctttg attgctgaat aataaatatc gactgcgtag
    11641 tctgtcgtac cgcctcctgg caaggttgca taagaaatca atccagggaa acggacacca
    11701 cgagtatcta cgccatattt cacataataa taatcacata gtagttctcc agctaccttc
    11761 gttaccccat acatggttgt aggacgctga agagtatctt gtggggtatg atcttttgga
    11821 gtatttggac caaacgcgcc gattgaactt ggcgtaaaaa atttcagctg gaattctcta
    11881 gcgacttcca atgcattgac cagcccagtc acgttcaaat tccaggctaa ttgtggtttt
    11941 gcttctgcaa cagctgacaa aagagcagcc aaatgaatca atgtgtccac ttgatattgt
    12001 tctgctatgc tgcgcatttt ttcgtaatcc attacatcta ggatctcaaa gatatcgttt
    12061 ttctcaatga cttcaggcat gcgtatatct gtcgcaatga cattttcact gcctagttct
    12121 tgacgtagcc gtgtgatcaa ctctgaacca atttgtccta aacatcctgt caccaatact
    12181 tttttcatga tgatcctccc tgatcttttt tactactatc ttcagatgat taacaatata
    12241 tgcgtgaaca tgttctctaa ttttaaatag acgaaaaaac taaatttagt taaataattt
    12301 ccatttcttt tcctattttt tgataaactg ctactgcttc atcaagcatt tccctcgtat
    12361 gttccgcagt cggcatattt ctgatcctgc ctgtcccaag aggcacagtt ggataaacga
    12421 ttggtttagc ataaatccca tgttcgatca gcttcttaga gaaagtctga gtcacttttt
    12481 cgtctcccaa aatgactgga gtaataggtg tttcagacat accgatgtta tagcctactt
    12541 ttttcaattc ttctttaaaa taattagcat tttcccaaac tttttcgaca agttccggat
    12601 gttcttgcat caaagtaatc gaagccaatg cagctgcagc tgcacctgga gttagcgaag
    12661 tagaaaatag gaatggccga cttctagctt tcaaccagtc gattagtgtt ttcgatccag
    12721 ctacataacc cccaacaaca cctatagctt tcgataacgt gcccatttgc atatcgattt
    12781 tgtcgcttaa tccaaaatgc ttgacggttc ctgcaccttt tccagttacg cctgagccgt
    12841 gcgcatcatc gacgtaggtg atcagtccat atttttcaac aatcggaata atttccggca
    12901 atcgtgcaat gtctccatcc atggagaaaa caccatctgt gatatacatg atttttttat
    12961 atttcttact ttctatcgct tcctttgctt ttttctcaag gtctttcata tcttgatgtt
    13021 taattcggat gatttttgct ccggataagc ggcaaccgtc aataatagat gcatggttta
    13081 attcatccga taagatagca tcttcttttg tcattactgc agagatagcg cccatattgc
    13141 agttgaatcc agattgaaag gcgattgctg cttctgtccc tttgaattct gcgatttttt
    13201 cttctaattg ctgatggatt ttcagcgaac cattgatcgt tcggactgct cctgctccta
    13261 caccataagt ttcggttgct tcaatacatg cttttttcaa ttcttcgcga ttggcaaatc
    13321 caagataatt gttggatgct agattgatca tcttttttcc gtctacgatg atacaagcac
    13381 cattttcact ttctagtatg tcgatcgtat tgtataaccc cttttctttc agttgatcca
    13441 attctttctc taaatacatt gttagttctt tggaagacat tgtctccacc tccatattca
    13501 tctgtcttgc agaacatacc aaacatatat cgtgtcattc atctacaaag caagtatgct
    13561 atatttttga taacgcttac actgattatg agaattattt aaccacattt ttt
//
LOCUS       DARDHA010000070        13556 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000070 DARDHA010000000
VERSION     DARDHA010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.070"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(369..995)
                     /locus_tag="SDA10_001607"
     CDS             complement(369..995)
                     /locus_tag="SDA10_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240515.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1290..1625)
                     /locus_tag="SDA10_001608"
     CDS             complement(1290..1625)
                     /locus_tag="SDA10_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEG4240516.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1612..1896)
                     /locus_tag="SDA10_001609"
     CDS             complement(1612..1896)
                     /locus_tag="SDA10_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEG4240517.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1952..3475)
                     /locus_tag="SDA10_001610"
     CDS             complement(1952..3475)
                     /locus_tag="SDA10_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG4240518.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3468..4643)
                     /locus_tag="SDA10_001611"
     CDS             complement(3468..4643)
                     /locus_tag="SDA10_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG4240519.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4647..4832)
                     /locus_tag="SDA10_001612"
     CDS             complement(4647..4832)
                     /locus_tag="SDA10_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240520.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4798..6492)
                     /locus_tag="SDA10_001613"
     CDS             complement(4798..6492)
                     /locus_tag="SDA10_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG4240521.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6489..6962)
                     /locus_tag="SDA10_001614"
     CDS             complement(6489..6962)
                     /locus_tag="SDA10_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEG4240522.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(7031..7186)
                     /locus_tag="SDA10_001615"
     CDS             complement(7031..7186)
                     /locus_tag="SDA10_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HEG4240523.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7320..7700)
                     /locus_tag="SDA10_001616"
     CDS             complement(7320..7700)
                     /locus_tag="SDA10_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG4240524.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7704..7919)
                     /locus_tag="SDA10_001617"
     CDS             complement(7704..7919)
                     /locus_tag="SDA10_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240525.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7922..8329)
                     /locus_tag="SDA10_001618"
     CDS             complement(7922..8329)
                     /locus_tag="SDA10_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HEG4240526.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8595..10070)
                     /locus_tag="SDA10_001619"
     CDS             complement(8595..10070)
                     /locus_tag="SDA10_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEG4240527.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(10060..10908)
                     /locus_tag="SDA10_001620"
     CDS             complement(10060..10908)
                     /locus_tag="SDA10_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEG4240528.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10945..11307)
                     /locus_tag="SDA10_001621"
     CDS             complement(10945..11307)
                     /locus_tag="SDA10_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240529.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11308..11466)
                     /locus_tag="SDA10_001622"
     CDS             complement(11308..11466)
                     /locus_tag="SDA10_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240530.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(11607..12231)
                     /locus_tag="SDA10_001623"
                     /pseudo
     CDS             complement(11607..12231)
                     /locus_tag="SDA10_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12228..12539)
                     /locus_tag="SDA10_001624"
     CDS             complement(12228..12539)
                     /locus_tag="SDA10_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240531.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12583..12876)
                     /locus_tag="SDA10_001625"
     CDS             complement(12583..12876)
                     /locus_tag="SDA10_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240532.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            13070..>13556
                     /locus_tag="SDA10_001626"
     CDS             13070..>13556
                     /locus_tag="SDA10_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4240533.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AY"
BASE COUNT         3781 a         2819 c         2139 g         4817 t
ORIGIN      
        1 ggaagagact gtgaagtcaa cgttttgacc tgattattag agaaacaaat ggcaaaatag
       61 ttcttcatat ttcgccgttt ttcgagcgaa tgccgagggg cagctgattg aacatcgttt
      121 atttggaaaa agggattgtg acaagacgtt tgtcacaatc ccttttttta tatcttatta
      181 attcattctt ttgttctatt cttcttcttc cgccatgaat gagaaaccca tatcaaagaa
      241 ctataaaggt tatcaaaaca gcgtttatga ctaaatgaat tagagttatc tatcactatt
      301 tttcaagcga tttaaaccaa atggcataca atatggcata caaaaacttc aataagccgt
      361 gggggttttt aaatttcttc ttgttcttta aaatcaatga gtggagctac ataatttgaa
      421 tccacttttt caaacgtcac tttttgcatc gacgaatgtt ttctttccca agataacgta
      481 aatagatttg actctggact tataaagtct acttcacctt ctagtattac tttcttatgc
      541 aaatattcct ctaataaata tagacttgct aaagcataca acacattttc caaatttgct
      601 tccttataat ttgaagaacg accatgtttt actctgttat atattttcca ccaatctggt
      661 gtatcttcag gccacccatc tccaaaaggt tttagtgaag tcaatgttaa agaattcata
      721 accctaacac cattatccaa acacattaat tcgctagatt taataacagc tttaaaatca
      781 gttatgcttt tattgctact gctatcaaat ccgcaaagtt ctttgaaaat aatctcacac
      841 tccgaaccaa tcgctagtag tagtttttga tattcaatag agaatgtttt gaaattattc
      901 tcatcgagca tcacaaaatc tgtggtatta ataaactcat tttctaaatg tagataatac
      961 ttccaataaa aatttgtaaa ctctgctttg tccattaaat cacctctttt gaattataac
     1021 aaaaaaaaca cgtccacaac agacgtgttc tttgatttct acacaatcag cttgccaaaa
     1081 ccatccaatc agtcctagca tgcctgtatt aaaattgaac gttcatcatc tatcatcttt
     1141 cttagtccag catgtttgca accacaccta caattttcag tccaacaata gatttcgact
     1201 agctaagtct tgcaaaattc aggcagctaa cccttcattt actaccttaa ttatactaaa
     1261 tacccacaac gtttacaagt gtaatcattc taagaaatct gtttagctat gatcgttgta
     1321 taatctttaa acgccgttcc ttcattgatt gaaataattt tataactctt gtttttccaa
     1381 gctactttca tatcgtttgt tagttcataa tcttgatcgt atcgaataat aaacgtcaac
     1441 gttccttcaa gaatggtacc tacactagca agcacgtctt ttagcatttg tgaacgcaca
     1501 ttagcccaac attcaaatac agtttcttca ctatccacca tctgcccatc ttcatctttt
     1561 acccgttttg tctttttaaa cttgactctc tcagtcaatt catttacatt cttaatgatt
     1621 ggcataataa gcacctctca tttgttgtac taatgcttgt accgttactg gtatccgttc
     1681 gctgctggct tcttgacggt tcaaatacca atgttgagta agtaatgaca ccgccaaatc
     1741 aaattgctga taattatcca tatctttatc cgttgcgcta ctatcaatag cactttttat
     1801 atattcgcct gctgtatcaa tcaattgttg aataaagtca tcatcaatag tgtgatcgat
     1861 ccgcatactt ttttttatgc tatccaaaat taccatttta tctctcctaa tgaaaaaaag
     1921 gcagtgggat aaaccgccgc ccaatatttt tctatacttt tggtgttaca actggcgtaa
     1981 actcaatata cactgaagca ttttgatcaa ttttcttata atcgttgcga acgatcactg
     2041 caagtccttg tgagtagtaa tcgaattttt cccattgagt cgttacttga tttctacgag
     2101 caacaaagat agattgtgca atatccccca taatcatagg gaatgttcca gctttaggat
     2161 tggcaaacaa tgtatctgca atcaatacta ctggcatacc gaataatgat ttaccactag
     2221 gtgctgtcac atcgggttgt aaaatgtaac gtccatctga atctttcaat gtatctaggt
     2281 ggtaatatcc gctttggttt agaattacca ttttatttaa tgctgggtct aatgccacat
     2341 tgtatagttg tttcaaatcg tccaacgtag cagccgtttt cttagtgaat gtttttaata
     2401 aatccatgat atgcttattg tcagtgtttt ctaccaattt cgctaactga tctttgacct
     2461 cttgtacaat attcactgct gaatcttcca caacctcatt tgataaggca atcttaccag
     2521 cacgagtttc tactttataa tcaactgaag taaacatttc agcgtcaata tcaccaattt
     2581 cagcaagttc agcttttgtt gctaacactg cttgttggtt agttgctact ggatatttac
     2641 cttgtccatt tgatactgtt tttactgttg catattgagc taagttataa tttgaacgtt
     2701 tcaaatcaaa gacttcaccg attacttctt cgggaacaac tgccgctgca tttacagtag
     2761 tgactccatc acgcacttcg ccttgtgaac ggatatattc ttcataccca cgcaattctg
     2821 ttttttcgtt atcgattaaa gttttttcca tgttattact ctccttttgt gctttagaag
     2881 tggattctaa gctattgttt gtttgttttg cttggttgtt actcataaac gattcgtaag
     2941 aacgcttatt tacttggaca tttgatgaat cataagcggg aacggtcacg acactaattt
     3001 cattaagtgc tttgatttta tctattgatc gttcaattgt gccatcttct tttttgtcga
     3061 atgaatctaa tccaagcacg aacccaaagg acatagaatc aacaacccct tttgagatat
     3121 tctcatacac atcatttgaa taggtcgtgt cggttagctc tgcttcaaaa tgtaatcctg
     3181 tatcatcgac attcaatttc aacgtacctg ctttgacact agctagtggc tttgaataat
     3241 cgtgtccata taataagaac acatttgata aatccacctc ttttaatgct tcgggtgtaa
     3301 tcacttctac aaagccgcct aaatcttttg acggttgccc gaatttcaac gcatagcctg
     3361 aaatagtttt gccgtctgtt ggttgttttt cattttcttt ctctactttg ggagaatcgg
     3421 ctgttagctc agcttcttct gtcagccgtt tttccttttc ttcttcatta ttcaactggt
     3481 gtaactcctt cctgttcttt taattgatat ttctccaacg tatctaaata cgtaaagttc
     3541 aaacttgcta gtaaacgatc gccaccatcc atggggggta gccccatttt cgaccgcccc
     3601 tcattaatcg tgagaagtga ccctttgacc tgatccaata cattttttac ttttgtttca
     3661 ggatcagtct ctagtaactg atcggaatta aagcggatat tggtttcaag tttcgtatct
     3721 aattcactca caaacacatt aaagtaatga atcagcgtac tttggatata ctggagatta
     3781 ctttgaacgg tgctagaatg ttcattttca acgcctaaac gctcaatcgg tacaccaaac
     3841 gccttggcaa tttgttttgt gttccaatca ttggaattga taagcttcaa aacatcggtg
     3901 tttatttcta atgttttgta gtccattgtt tcatcaagaa tgatcgttct aagagcatta
     3961 tctccactac ttgaaccgtt ggcttcttca aacttttcac gaattgcgtt ctttgctgat
     4021 ccgtctaaat ctgacttatg aaccttcaaa atacctgacc cactgacacc acgagtaaag
     4081 aaattgtgca atgtgcggtt gccagcttgt tgaattttca gttcatcacg taacgcataa
     4141 agtggtggta atcctgttaa accatcttgg gtgaaatatt tgaagtgcaa aatatcgcta
     4201 gacttcacac gtcttttttt atcaccaatc tgataagaca acgtaccatt atctaattga
     4261 gtaaccgtta cacttgagtt aggtaataag tgaatttctt ctaccttttc accgttgcgt
     4321 ttaatctctg caaagctatt accgtttagc aacatattga ccgccaaagc aaactttaaa
     4381 tgccaaccat ccatttctga attaggtttc tcgtttagta acttcaccag ttcattatca
     4441 gcttgtggca tattgttttt gaccaattga atcggacttg aagcaatatc actagcaata
     4501 attcgaacgg ctgcaaacac atcactattt cttaatgcac gaacgcttgt aaagctggtg
     4561 gtgtaatcat ccgattggat tgataccaca tgttgtagga aaggatctcc cgttgtattt
     4621 tgtctcgctt taaaaaatgc catcttttaa cctcccttct gttctgtatt tagcatgacc
     4681 gctaccccaa taagcactat gcccagcgct aacaagccat agtagacatt tgttagtaag
     4741 gtgatagcaa ccaacacaca caccaatcca agaagtaaaa gaacggtatg aatgtaatta
     4801 gaaagaaaat tcttcacttg tataaaattc attgtctgct ttcgctcctt ccgttgtggt
     4861 gaagtaatcc attgcaaaca cataggcatt gattaatgca gctattggat caattttgtt
     4921 actgttcttt gatttattaa tctgaatgcc gttgttatcc gtttttaaaa tagcgttgtt
     4981 cactgcatgg gtgagaatcg tatttttatt gtgaataacg ttgccttcat aaacttgttc
     5041 tctaaaagtt cgggtcggaa cgtttagagt aatcgttcct tgtcttactt ctaacattgg
     5101 gtagttggct ttttcagctt tactgataag cgaattagcg ttatacgggt cataacaaat
     5161 ccccatacat tccaaatcat tttcttgaat caaatcttga ataaactgaa atacttggtc
     5221 atagtccacg attccacttt ctaattgagt aatggaacac tcaccagctt tttctagttc
     5281 tcgataaggc aaaccgtcgc gcttttcttt gtcttgaagc ccatatttgg tggctacaaa
     5341 gctatgtgaa tcacaataaa gttcgccgtt atctagcggt acgatccatg aaacgctagt
     5401 taaatcatct gttttagata aatccactcc gatataaacg ggcttgcctg taatatcttg
     5461 tggttctact tcggttgcct gccagtcttc agcaatcata tagctgtcct cactcgcttg
     5521 tctccataaa ttgaagtttt tcaccaatac agaattaagg ttattttgtt tcaaagcaag
     5581 gttcacatca tcttgaatgg ttggaatcat tacttttttg atttcttcac tttcaaaaat
     5641 cggattggct ttgatccatt tctcttgatc gtgaatttct tcgggatcgt ccaattccca
     5701 tatcgctata aagtacctgt cggcttgttc ttcgcctttt agcacacgat ctaacaataa
     5761 gtattcctcg tacataggaa cgtttaaatc aagcccagcg gtactaataa cacacaataa
     5821 gccgttcttt tgttgggtca ttcctgactt aatgacgttg tacgttttac gagtttttgc
     5881 ttcgtgccac tcgtcaagaa ttgcggttgt tggtgcaaac ccatctagcg tgctggtttc
     5941 actagccagt gccatagcga acgaattaga gggcaagtgc tgaatcttag agttcataat
     6001 tttcagttgt ggtcttaaaa acttactaga cttgaccacg tttctaagtg aattagacag
     6061 catatcatag cccaatttag cttgtttcaa agcgttactt acaaacaata cttgccttgc
     6121 ttctgctggt tcttgttcca taatcaacgc attggcagcc atgccacttg cgatatacgt
     6181 tttcccattc ttacggctca tactggtaaa cgctcgattg aatcgcctgt aattcccttc
     6241 tttagtacgc cagccataaa gactgcctag aatgaatttt tgaaatccta acatcttgat
     6301 tgctttaccg tccgtagatg gtagcaactc aatgaattta atcgctttgg tggcttgttc
     6361 ttcatcgaac acataaggga agctatctga cttagaacgc tccaaatcgc ttatatgacg
     6421 ctcacacgct tggataattt tcttgggtgc aatcagctta cctgatagga ctttctcaat
     6481 gtaatcattc atgaattcat caactctttg aacgggtctt cgggttcatc atttttaacc
     6541 ttgttcaaag ctagttttga tcgtgattct aaggtcatac ctagtgaggt ggcttgtttt
     6601 ttcaaatcac tcatagcttg tgattgaacc cgaaccgctg gattggcttt ctttactccg
     6661 ttttcgcatg tgataaacgt acctgatttt cttatctcgg cttcggctgt tttcattcga
     6721 gcaaaggcta aacaataggc taccaatgaa ccataatctg cttcactcaa tggataatct
     6781 ttttttaatg ttggtacgat acgattccat tcacttctag cgctcgctgc catccagtca
     6841 gggggcgtga tatttattaa tggttgctgc ttgaacaatt cttctctagc gtccacacgg
     6901 attgcttttt cttcacttga tatattgcct tttgtatcgt gtaatatttt agggtgtccc
     6961 atgttgtcct cctttcagac aaaagaaaaa ggaacgtaag caattaagct cacattcctt
     7021 cgtttttcga tcagaatttt tctttatcta atttatctat ataaatgatt tttccgttct
     7081 gcatttgaat ggtcacttga ccaaaatgag gtaactcaat ggtgcgctgt ttccctgttt
     7141 tatcagtaat aacaattgta tttttcaata aatctcctac tttcattata tactttttca
     7201 cttgtgatta caagtgtaat cgttatttgt aattaccctt atgccttttc acaacggtta
     7261 tttgtaccaa aatgagtagg catcgattgt cagtaagttt cacactatgc ccccttaact
     7321 caaagggtac tcttttctcg tctttgtctt tcattggctg tcttggaatt atggtgtgca
     7381 tggcataaag aacgaaaatt agtttcatct aaccgtctat tccaatcatc ttttagttca
     7441 attttgtgat ctacaacatc agctttcctt atcaagcctt cttctaggca atcctcacat
     7501 actggatgat ttaaccgata caagtatgaa gcctttcgcc atgttctact ttgatagaat
     7561 tgaaaatatt taccttcgct ttgctttctt tcttcacgct taattgccgt ttgctttgct
     7621 ttatgcttcc cacaatactt ctgcctataa tcaatcaatg ttttacatcc agcatgatta
     7681 cattgttttt taactgccat agattaccaa ccaaagcgat ctcgttcgtc agaatcatct
     7741 tgttcccatt ttgatttgta ttcaatataa ggcgaaacta tcttcaagca cgttccgtct
     7801 ttaaaattaa ctttagtgat tttatcttcc tcaaaactaa taaagtctac atcttcttga
     7861 gtgtttgaat actcaatgtg tttctcacca tcgaagtatc taatactttc catatgcata
     7921 attattctac ctccaataaa ttagttatca attcatcatc tgttagtagc aaagccaaga
     7981 acttatcatg gatgtttaaa aacaaattat cctcattcga tgtaagctct cgttgtctcg
     8041 ctcgatcata taggttaagc agacaattaa attcatccat tagctcacct gtatcatcat
     8101 agttgccact atctaccaat gatcccacta ctagcgattg acaccacgct actgactcct
     8161 gcagccaatc aggttcttct tccattatct ttaatacttc tggatcaaat gtggaataat
     8221 cttctgttac caatcccaca ctaaatgcca gcgtgttcat tagaaaccga tccatttcat
     8281 ctgtatattg aaagttcggt gctttgtttg ttgaaataat attgagcatt tactttcctc
     8341 cttgattcgt aacacgtggt aacacctacg taacaggtac ccgttaccac tcaatcccaa
     8401 cggggacaag ggttctagcg ttttggtaac aggtaacggg taaaatccag taacattatt
     8461 ccccagcata ttagctaact atttcaaaag ttttaaggaa ataaggtgtt accccgttac
     8521 caaattgctg tatcccttgg ggcagtaagg gtaaactagt aacgggctag gtgttactga
     8581 cccgttacta cccgttactt tttgtacttt ccagctcgat ttttattttc ataggcattt
     8641 gacttattgt aaaatcctcg tcgtttattt ctttttggca gttgcttccg ttcccaacca
     8701 ggaatactgc taataatcaa ggcaatcttt ttagtttctg catctgatcg accattcaat
     8761 aaatcctttg cttgtttatc aaaaacagct tccaaaatat cagcggtgag tacgctatca
     8821 actaaataaa actcttgttg catagtagct ccattataga aatgctgtaa atttttctca
     8881 ttacatttac tgtcaggata gttctgataa taagaacgtc tcgcccacgc tggagcttct
     8941 tcccatttat acgggatttc catttccaaa tatttagtga ttgcttcttt aattggattc
     9001 tccatcttgg catcctcttg atagtctttt gcaatttcta agacttcttt atcttcatcg
     9061 ggactaaaat agattcgttg tcctttatca aaaagcacct ttgcttcagc taacacttga
     9121 agaaaatatt catcctttgt tttaaacgga ttcatctttg gcttattctt acaaggcaaa
     9181 ggataaaaac ggcggtttcc tgtatcatct ttcaaatact gcccatcatt cgacgttcca
     9241 ataaatacac attgccttgc ccaagctatg acattccgac cataaggagg tctaatttta
     9301 tcgatcttgg cactgatgaa gttttttatc tgttcaattt tagttttgtt cattgaagat
     9361 aattctccaa gttcaataat tacattgcct tgaagctgca tataatcatc tttcttttca
     9421 cctagcgaat ctaatgaatc ggtgaaaaaa tcatccgtgt aaagtaatgc tggaacggta
     9481 ctcttaccaa tcccttgcgg tccatccaaa actggaacga tctcaaactt tattcctggt
     9541 ttatagatac gtgcgactga tccgactatc caacgttttg ttacttctcg tgtgtaaagg
     9601 ttgtcgtcta cccctaaaag gtcaataaac agtgtttcca gtctaggctg ttgatcccat
     9661 ttaacttttt cgatacgttc ttttactggg ttgaaaacat tctttctagc cacatttact
     9721 actgctttgt gtattttgtt gtcatcaaaa actacattgt agttttcttc aatatatgcc
     9781 gttaaggcat cctcaaaact gtcttcccat tctccagcgg tacgattgat ccaaggagaa
     9841 tcttttaata ggtgaatgta tccgctaaat tcgttataag caattgttcc agctaaattg
     9901 ctatcattgg ttaaaatcaa ttctgcattc acaacactgt tacttttaat ctgatcgtct
     9961 ttattgcatc ttaatcgtga catccaggtt ttcaattcaa tgatattgtc ttcacgctcg
    10021 ctggcttcgg gaaagtctaa aatactatct aatttatgct tatgagccat gagctttccc
    10081 cctcaaatct tttttaatca tcgatcgaat aatttgattc acttctttat cttttagcgg
    10141 tggttcattg tacgcatttg ctgtataaat caattctgct aaagtttgat tatctacacc
    10201 gtgccatagt aaccaaccgc ataacttagt taaatgatcg tttctactac cttcgccagc
    10261 gccttgaaca atcatatcta atttttttgc tgtccctgtt tttgctacgg gtcggtattg
    10321 agtctttatt tcttgcggtt tttttagcag taggttcact agccaatcgg gagctggtgc
    10381 aatattattt agagatacgt tttcaatagg ttcatatgct ctcccaacac ttcttgaaat
    10441 aatcatagtt ggcgctattg gaactccttt agtgataata tcaatcccgc ttaaactttt
    10501 acctttatca tcacgcccga agatatcagc aaatgcacct gtttcttgtt ggataattac
    10561 accatcagga attttaaaga agaaatggat tcctttacct ggtgtgattt ccgtataagt
    10621 agaaggaaat ggatcataca ttgaagcaag ttttttataa ttctcaacgc cgtttcgtgt
    10681 ttcagaatgt tgatctaggt caactaccac catttgatga tcttcaagca ttaacccaat
    10741 atttctagtt gcatcttccg tccaccattc attaatttga ttagaatcgt tagttgcatc
    10801 attccaacca ctagagcctt ttattggaat tttagttcca ggagataagg gataaattga
    10861 ccatcccgac ttttgtaatt ccgttgctgc ttgataatta tttgccaata aacctctcct
    10921 cctttttcaa aatactggta taaatcattc agacacctcc atgagcttca actgctcaag
    10981 ttcgtatttc aacccatccc gaacaaattc agcacctttt aattgattct gatactttac
    11041 ccatgtatta aatgttttca cccttgcttc ttgcatatca gtaatgttta accatgcggt
    11101 taaaatctga ttttttagct cgttgatttc tcgacaaatt attttgtgtt ctgcatgttt
    11161 tttgagtaca tacaaaactg ttagtttttc attgaatgaa tttccaaagt aggttttccc
    11221 gatattattt tctagttcag cgatcccttg attaatttcc tctaattcaa ggctatcgag
    11281 ttggtttaat agttttgttt tgtacatcta cttatgccct ccagttctta attgatagtc
    11341 gtactcgtca taagcagtga agagatataa cgctaatcct gcaaagatag ctatttttgc
    11401 aattaatggt aataagccga aaattccgcc gattaaaatg cttcctataa ttaagtttcg
    11461 tttcataatg gttccctccg attttttaat atggtataat cggaacaaag aatatcgggt
    11521 tttatcccaa ttaagttgcc tttatcctgt ccgccaagac tgttatggat aaggcttttt
    11581 ctttgttctc gatcatgtta atgctcctaa gttgtctgca attcagcaga acgattataa
    11641 attttctcac tcactgaaaa ctgcaaccca tatttttctt taacagtcat taattctact
    11701 gtctcatcta aaataggttc acgatcaacc aacatttcag gagtcatatc tttctttttt
    11761 accattttgg aatgaccata aatagttgat acagctttat tggctatcac attcgcttta
    11821 atgtagtcta ctggttttgg ttcagtaata gcgtggctta atttagtcat tgcttctttt
    11881 tggtgttctt tgtctagcat gcggaatact tgaaagcctt caagtcctgt ggactggcgt
    11941 agttctttga tgatgtcgaa aatccagtct tgaaatgcct cggcttcttt tttacggctt
    12001 ttgaatgcca tgcggtaaat attttttttc gttgatgatg tttacatctt gtgttctgcc
    12061 caatgaatcg atgaccttac tagtagtaac cccatcttta ggcaaagagt gaatggctct
    12121 agtaacctgt ttaagaccta acgcttccgc aacatctttc gccactgccc accattcatc
    12181 attaatatcg acaaatctta taatgtgtcc gttccaaatt tcagttttca ttcaacgtcc
    12241 tccagttcaa gccctagcaa gctcgctaac aactcaactt gctgatacaa aatttcttga
    12301 tactcttcaa tagaaagtcg ttctttaaac tctgcttgta ctaaatcgtt caagccacgc
    12361 aatactccgc ttaccgttgg ttcttgtggt agcaattttt gatagtccat ttgttcgtac
    12421 aaacattcaa acgtttcttc tagcgctttg ccttccaagt ttgctaattc tgccgctgtg
    12481 tcttctgtaa gaattgttga taatactttt tcgattgctt tattttgtgt cgattccatt
    12541 attatttcct cctattaatc cctttgcccc cacggcttgc gtttattttt ggtattcttc
    12601 cataaagcga tcacattcat cttgtgaaat tcttacgact ccatcaacta taatcaactt
    12661 taaaccgtta ggaacatatt tctgcatcat agtattgtat gaaacgttca tatacttagc
    12721 agcttgtgcc ttgttcatat attttggaaa ctggtacttt ttttctaata atgaaagtaa
    12781 cgttccttga gtttgctcta cttgttgaac aactcgatcg tcaataattt taaaagctgg
    12841 atcatcaagc gtgagtttgt caatttttaa ttccatattt atcccccctc gttagcatac
    12901 atttctatac aaagatttta aaaaaatgca agaggctctt gaatacattt ctatacctct
    12961 tataaactca atataccatc ataccttttc atttgcaaga attttaacat acttttttga
    13021 acttttatgt ttcacacata tttaaatcgt gatatagtag agaaaatata tgaatggtgg
    13081 tgaaaatgtg gctagaaaaa aataccccga agcagtggag acaggacaaa gaattagact
    13141 ggctagaatt gaaaagggac tctcaatgga acaacttgga ggtaaattat ccccaccggc
    13201 cagtaaaggt gccgttagta attgggaaaa tggctataat cttcccaata acaccaggct
    13261 taaacaactt tcaaaggtat tagatgtatc cactacctat ctattaaacg gatcttatac
    13321 attaagagat atgcatatga tgcccgaaat taagcgaaat gtgcttgaaa agcaattgaa
    13381 aaaaactatt gaaaaaaata actctataat tgattctaat ctttctaatg ctatcaataa
    13441 catcgaagaa gataacctga ctgtctcaca gaagatttat ttaacaaacg caataaaata
    13501 tcttaaaaca ggatctcgaa ttgaatttcc tgacgggagt accccaattg cttatc
//
LOCUS       DARDHA010000071        13454 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000071 DARDHA010000000
VERSION     DARDHA010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.071"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            105..272
                     /locus_tag="SDA10_001627"
     CDS             105..272
                     /locus_tag="SDA10_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEG4240534.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(334..2145)
                     /locus_tag="SDA10_001628"
     CDS             complement(334..2145)
                     /locus_tag="SDA10_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEG4240535.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2142..2507)
                     /locus_tag="SDA10_001629"
     CDS             complement(2142..2507)
                     /locus_tag="SDA10_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEG4240536.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2670..3065)
                     /locus_tag="SDA10_001630"
     CDS             complement(2670..3065)
                     /locus_tag="SDA10_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG4240537.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(3083..4045)
                     /locus_tag="SDA10_001631"
     CDS             complement(3083..4045)
                     /locus_tag="SDA10_001631"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEG4240538.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(4045..4257)
                     /locus_tag="SDA10_001632"
     CDS             complement(4045..4257)
                     /locus_tag="SDA10_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEG4240539.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4278..4976)
                     /locus_tag="SDA10_001633"
     CDS             complement(4278..4976)
                     /locus_tag="SDA10_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEG4240540.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(4996..5538)
                     /locus_tag="SDA10_001634"
     CDS             complement(4996..5538)
                     /locus_tag="SDA10_001634"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEG4240541.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5670..6674)
                     /locus_tag="SDA10_001635"
     CDS             complement(5670..6674)
                     /locus_tag="SDA10_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEG4240542.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6671..7660)
                     /locus_tag="SDA10_001636"
     CDS             complement(6671..7660)
                     /locus_tag="SDA10_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEG4240543.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7657..8463)
                     /locus_tag="SDA10_001637"
     CDS             complement(7657..8463)
                     /locus_tag="SDA10_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4240544.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8629..9585
                     /locus_tag="SDA10_001638"
     CDS             8629..9585
                     /locus_tag="SDA10_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4240545.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9662..10180)
                     /locus_tag="SDA10_001639"
     CDS             complement(9662..10180)
                     /locus_tag="SDA10_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG4240546.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10268..10417)
                     /gene="rpmG"
                     /locus_tag="SDA10_001640"
     CDS             complement(10268..10417)
                     /gene="rpmG"
                     /locus_tag="SDA10_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG4240547.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10645..11091
                     /locus_tag="SDA10_001641"
     CDS             10645..11091
                     /locus_tag="SDA10_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEG4240548.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11286..13181)
                     /gene="asnB"
                     /locus_tag="SDA10_001642"
     CDS             complement(11286..13181)
                     /gene="asnB"
                     /locus_tag="SDA10_001642"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEG4240549.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         4193 a         2845 c         2292 g         4124 t
ORIGIN      
        1 ttttcaaggg ttttttgcaa aggtttacaa acaaacgtaa aactagcctt tttttctctt
       61 ttgttccata atagatataa caaataataa atgaggtgga ttcaatgaaa aaatttgtat
      121 ctggaatatt agtaggaagt ctggcaacag ccgctgccgt tgctggtttg gttgcttctg
      181 tgaaaaaaac agtgatcgat ccaattgacg aaaaagaagc aatgatcgaa gaaaaccgta
      241 aaaaagcaat gcgtaaacgc gtttctcgtt aataattatt taaaaatacg catgaagact
      301 ttctaaaaaa cagataagtc atcatgcgta tttttatttg tcagtcaata taagttgtgc
      361 tattttatct gcctgtctca gattccatgg atctgtataa ggtctagcta ctgctgcagt
      421 tagccaatcc acaccttcaa caggtattcc atagcaataa aagtcagact ggatttcccc
      481 tgaagcatca atcacgcgat tcgtacctcg ctcaacatct atagcttctg aatgataact
      541 tcctgtatta tctgaaaatg aaaaagggcg aacaagtttg ttttccttca aattttttat
      601 gacaggatta cttgaacgag aaaagcttgt ttgtggcaat cgagcttcaa tcaaatagcg
      661 actgctgatt tctttatctt gtagagaaat aacatagcgg ttgtgctttt gctgaatatc
      721 aaatggcggt tcgatcaaat gaaaaatacc cgcatctatc agtgctgcta attctttcgt
      781 ccgaatgatt ggcggaccga tcgttaaaaa gctatttaga tgagtaaacc atccccatag
      841 tatttctttg tattccttag cagaaaaaac gtgccattca atagcctgcc ggattggatc
      901 acgccagtct tttaatgcat caaatgttga agcaaatgct cctgtgcaat tgcctttttc
      961 cgcttctttt atctgccctt caataaatat tcggctagct tctgaaaagg gctcattctg
     1021 ataggcaaga ggagtttcta aatacgacca atcccatcta taagggatta gtgctggatg
     1081 ctttccgaga cactcctctg aagagaggga gagaaaatca tgctcaaaat ttttctttgg
     1141 aatcggcaag tgatattctt cgatgatttt cttgtaataa aacaactcaa cgtctttttt
     1201 cagatagtca aaaaatgttt cgccagtcaa aaatcccgac tgatgccatt gctccaggtt
     1261 ttcttttgtt agtaaccgtg gccatgccat tgctccgcct tgcttttgat ttcttcctcg
     1321 agggtaataa gggagccccc ttcttgatcc actgtataca atcggttcct tgcctgatgg
     1381 atgatagacc agttttccct tttcgttctc gaagcgccct cctcttgata ctgtaaacaa
     1441 tccgacataa tcaaaaaaac ttaagcctag acctcttaaa aagacatttt ctttaggcgg
     1501 tatcttcgaa agatctacat ctgcaggatt agaaggaagc tgatacagta aaacattttt
     1561 ctttgcgtag tctacccaca ctctttcttc ttctgtctca tcattttccc agtgaccgct
     1621 tgctaaaaca agctgatcga tagaatagct ttgctgatct gtctgtaaaa gataaccatc
     1681 ttttcttttg cttacttttt ttactaaaga ataaacaaac tgaatagaag ctggaaattc
     1741 ttcttgcaac tttgagaaaa accagcgttg atagagtccg tataaacatc gagtagattg
     1801 ctgatctttt tccagacgat tgatttcttc tataattagt tgcttatttt tggttaccgt
     1861 ctgttcgata taatctgtag caggtccttt actccattga aacaagttgg gtccctgtgt
     1921 taccaatcct ccactggaca atgtttcatc tgtaaaaagc gtcacttgtt gagaaacaga
     1981 attcattaat aactccggtg gctgattcaa ccgccaaact cgacctccag gaccattggg
     2041 atcgaaaaga aaaagttcaa gagaatgtct gtcctgatta ttatggagaa tcctttctgc
     2101 tacacttaat cctctaggtc cggcaccaat gatccctatt ttcatttgca tgcctccatt
     2161 tcgataaatt ttttcaataa aacagcatgg ttctgatgtt cattttttgc accataaaca
     2221 aaagtaatga ctggatgttc tttcataagt tgactgagtt taaaaatagc ttgcttcttt
     2281 tcttcttcga aaatcaattc ttgctggtat ttctttgcaa atgaagcaaa tcgttctggt
     2341 acatggtcaa aagttttgcg tgcttcatta ctaggtgcaa tttctttcaa ccacagatct
     2401 aactgcgctt tttcttttga aattcctcgt ggccagaatc tatccacgag tattctatag
     2461 ccatcctctt tttcagcatc aaggtaaaca cgttttaatt tgatcatagt tgtttccttc
     2521 tttctacctt ttcgcttagc ttatatatgt agataatagc acaaaataaa aaaaatgaac
     2581 ggcacaacta tccattgcgc cgttcatgtg atcttggtca aaatctaatg attgattcgg
     2641 tttttaaagc tgtcatacaa tcatctgttt tatacttttt tacttttgat ttttccagac
     2701 catccgtcat atccaccctt aagtaaataa atctcttgat aacctgcttt acgtaattta
     2761 tttgctgtac ggatgctcaa acttttcttt tgatcgtaaa tatagacagg ctggtctttt
     2821 cgaatagatc ctaatgaatc tttcaaaaca gtgtaaggga tgtttcttgc acctaaaata
     2881 tgcccagcat caaacacatc tttttcgcga acatcgatta cttgtgcttt tctcattcct
     2941 tctcgaaatt cttcttctgt caaagtcgtt gctgaacgtt tggccattac tcgtagatag
     3001 acttcccaaa ggatgattgc aagcacaatc aatagcaaaa tgatattaat tacccataaa
     3061 accatgtaat gcctccgttt ttttaagcaa atttcaaggc taatgaggca gcaccaatga
     3121 caccagcttc attaccaagc tctgctaatt tgatttttgt tgattctctt acttgcggga
     3181 aagtaaattc tttaaaatat ttctctacac ggctacgtaa aaattctcct gctgccgata
     3241 ctcctccacc taacacgata cttgacggat ttaatgtatt ccccaagttt ccgcaagcta
     3301 aacctaagta gaagcatacg cgatcaacaa ccatcaatgc aaacggatcg tcttcttgag
     3361 ccatttcaaa gatatcttta cttgttactt cttctccgtt gtctagcatt gctttcagtt
     3421 ttgaatcccc tgcgtattct tctgccaaga aacgacctag gcgtaccaca cctgtagcgc
     3481 tagatacagt ttctaaacat ccgcgtttac cgcaagtaca ttcgaaccct tcaggatcca
     3541 cagtgatatg tccgatttct cctccagctc cgctcacgcc gtgaatcaaa tttccgccag
     3601 caacgatacc tccgccaaca cctgttccta gagtaacgaa tacaacgtct ggatcgtttt
     3661 ctccagcacc tttccatcgt tcacctaaag cagcaacatt cgcgtcatta tcgatagcga
     3721 atttgattcc tgttcctgcc tcgatcagtt ttttcacaaa ttgaacttct gtccagttta
     3781 agttatacgc accaataacc gttccagctt cgctgtccac gcttcctggg gtacccatgc
     3841 cgattccgat aaagtcttcg gctttcatat tatataaatt caaacgatga ttgattgatt
     3901 caatgatttc aggaacgata tgcttaccat catccaaaat attggtatcg atgctccatt
     3961 tttgctgaat ctctccttct ggtgttaaaa ttgcaaattt tgctgttgtt ccacctaaat
     4021 caatcccaat aatcttcttg tccattacaa atttcctttc tttcggcttt cttcaattcg
     4081 gtgctctctt tttaatatgc gccacgctgt catgtatgta tcatgatcaa tcaaacggct
     4141 atcatacaga ttacgcaatt cgatagtcat taattcaatg tcatagatgc gtgcacccac
     4201 atagataaag atgccaaatt ttttgaacag ctgctgtaca tcatataagc tctccatctt
     4261 taagcacttc cttcatatta tacaagtaaa ccatattttt ttaaacccag taaaatacat
     4321 attacaaaaa gaaatacaaa aactagtgta gaaagaatcc tttggtgtat cgaatagctt
     4381 gtctgttgtt tcggtagacc caaaacattc cctaacacta atccgccaat tagcccaccg
     4441 acatgtcccc aaacatctac agaggtatcc agaagaccaa aaacaaatgt caacacaata
     4501 aataaaagaa aatgtctgac tagctgctgg atcgctgggt tatgtttaaa atgaaaccct
     4561 aagataaaca acgcaccgaa caaaccgaat atcgaagtac ttgcaccacc agacagaaca
     4621 ttcgcttcgt taaaagcaaa gcttgctgta tttcccatta tcccgctgaa catataaatc
     4681 aagaaaaaac gccaatggcc atagattgcc tcgatttgct gccccataaa atacagtatc
     4741 agtgagttca ttacgaaatg catcagtcca aaatggatga agattggtgt cactaaacgc
     4801 caccattcat tgaagtgaac aagataagga ccatacattc cggcataata agggatctca
     4861 agtcgcggaa ataattccat catgaggaag accgctgtct gtatccctaa aaaagcatag
     4921 gtccataacg gtcgtcttaa ccatgcacgc aattttattt gctgttgata attcatgaaa
     4981 acctcccatt tcattttagt caataaacaa ttgttggatc ttctgatcaa agctctccgg
     5041 ttgccattgt tcattcaatt gttcacgaaa aacaaggcta catgtttttt tatgaaaatt
     5101 atttaaatac cggtcgtaat aaccaccgcc aaaaccgatt cgataaccgg ctctattgta
     5161 gataatacct ggtacaatga ggagatcgat catctccggc aaatagagct tctctgattg
     5221 tggctcaaga acaccaaaac tcgaacgtac aagaacggac tgttcatcca tttcataaaa
     5281 cgccatctga cgtttcggca aagttttagg tacaactatt ttcttttgtt ctttttctgc
     5341 tcgttggata accggctgtg tatccagttc aaattcattc gataaagtcg tccctatcac
     5401 ttttgctgac ttccagtact cgctggcgaa tagctgttta tagattcgct gttctttttc
     5461 ctttttcttg ccacttttgg ccagattttt cagcttttcg attcctttcc ggcgtaatct
     5521 tactttcaat tggtccaaaa actcaccctt ccttctctca taaaatggta taggaaaaaa
     5581 agaaaaatag caatcgaact ttcaaataaa tgcataaata gaataaaaag aagacctaga
     5641 aatgattggc cttccttttt taatgaattt caactgttca gcgcgtgttt ttgtctgata
     5701 aaaagtgcat acagaaaata tggcgcacca aaaagggcaa tcaaaattcc tgtaggtact
     5761 tccccacttg gtagaatcac tctagcaacc atatctgcac taagcagaaa aatggaaccg
     5821 aacaaaccgc tcagaacaaa ggtccagtta ttttctttac gtacgactag cttcgcagca
     5881 tgaggagcta gtagtcctac aaaagataaa ctacctgtca ccgctacact accgcttgcc
     5941 aaagcaacag ctaacaacaa aaggattttt tgacttttag ccacgttaag gcctaatcct
     6001 tgcgcgcgtt cttgaccaaa tgatagcaat acaatcctct gtgcataaag tcctcctagc
     6061 ggaattaaga taaccgccca tggaagcaat gcccaaacat aaggccagct cgcaccccag
     6121 attgatcccg ctaaccatgc attcacaaat ccataatttt ccttagataa ttggatcgtg
     6181 catagcaatg ttaatgcaga aaggccggca ttaacggcaa taccggacaa taagattcga
     6241 ttcataccca aaaagccatt tcgatcatag gccgcaagat agacacagca agcagctaaa
     6301 acaccaccta gaaaggctac aactagcaat agaaacggca gcgaatagtg cgaaaagaaa
     6361 cctaaataaa gcatcactaa aaatcctgct ccggcattaa tgcccaatat actagaatct
     6421 gccaattcat ttcttgttac tccttgcaag atataaccag aaacgccaag acaagctcct
     6481 gctaataaac tgatcagtaa tcgaggaact cgaaattctc taacaatcag cgccaaagaa
     6541 gatgattctt tccctatgaa cgtggcaaaa agctgatcca ggtaaataaa aggcgtacca
     6601 atcattaaac tacctataga caggatgaca agtacaaaaa gcaagaaata gaacgtccaa
     6661 tatatttttt tcaccgttgt tccctcctta cttgtatcag taaaaacgga acgccaatca
     6721 atgcaatgac taatccaaaa ggtgtttcaa aagggggatt gatcatcctc gctgtaaaat
     6781 ccgccaacat aaccaatcca ccgccaacta aaaaagagac aggtagaaca taacgatagt
     6841 ccgttccaac cattccgcga acaactggcg gaaccaacag cccaataaat gaaacaggtc
     6901 cgactaacga aacggcaatg gctgctaaca ctgcaactag acagaaaaca atcaaccgcg
     6961 ttcgtttagg atgacttcca agagctaatg catgtgtttc actcaaatga atcaaagata
     7021 ttgcttttcc taatgaaaaa gaaaccaaaa cagcaagtat atataaagga aacaatactg
     7081 ctaattgttt ccatgtgata ttggcagctc cgcctacaaa ccaaaaagca agatcctgct
     7141 gcagattaaa gaattgggta agtgcttggc tgatagcagt aaaaaaagaa ctaatagcta
     7201 cgccagctaa aatcagctgt aaaggattca tgcccatttt cgaaaactgc aaaaaaccaa
     7261 atacgagcaa agttacagca aaagaacctg ccaatgacat tagaaacaca gaaaaatcag
     7321 aagctttagg ccaaaaagca aaactaaggg ccattgccaa cgaagcaccc gcgttgattc
     7381 ctagtaagcc tgaatctgct aaaggatttc ttgtcacccc ttgcatcaat gcaccgctca
     7441 atgcaaaaca actccctaca agaaatgctc ccgccacacg aggcaaacga atatttcgta
     7501 tgatttggtg actttgtttt gatctatcaa aatcaaacaa cgcctgccaa aaatctgctt
     7561 tttccacagt cgctgcgcca tttgaaagag caacaaaaaa aataatcaaa agaacaaaac
     7621 agaccaaaag caggagtcta tttttcttca tagtcctcat aattgatcct cctgctcttt
     7681 tggcaacaaa tcataggtta gaaaaactgg tttatttgtt cttggaagcg tgacaatcgt
     7741 tgcatcaatc tcaaatattt ttttcataca ctcttttgtg atcacttctt caggtgtacc
     7801 ttctgtgatc aacttaccgt ttttcattgc taataaataa tctgaaaaac gtgaagcatg
     7861 attaatgtca tgaatcgaca tcaagatcgt tgtttgtttc atttgattga tttcagtcaa
     7921 taactgtagg atctctaatt ggtgtgccgg atctaaaaat gttgtcggtt catccaagat
     7981 caagatatct gtttcctgag caagcgccat agcgatccag acacgctggg tttgtccacc
     8041 agacagcgtt gcgatatccc gcatacgaaa tccagctaga cctgtagcat ccaacgccca
     8101 ctcaattgct tcatgatctt gcgtgtccat gccactgaac agtttttgat aagggtatcg
     8161 tccgtaagat actacttctt ctactgtcat acctgtaggg tgttcactag tttgtgctaa
     8221 taatgcaatt ttttttgcaa gttcttttga actgtatgta gagatatcag agttattgac
     8281 aatgatcttt cctgatttca caggtagtat acgggtaaaa cttttcaata aagtagattt
     8341 tccacttcca tttggcccaa tcaaactagt gattttccct tcaggaatcg taacagacat
     8401 gttttcaatg atttttttgt tttcgtagcc ggctgcaatg tcagctgctc ttaatctcgt
     8461 catttcaccc atcctttcgc tgaaaatcat tctcaattat gacgctgttt caacatttcg
     8521 tcaagcgcta ctttcatatc tatagccaaa tgaagcggtt gacagcgttc gaaatatcag
     8581 gaataataac caatgagaaa gattctcaat tatgttagga ggaaagaaat gaaattaaaa
     8641 acaaaagtat tcacattatc tagcatattg ctgctggctg gagtactgag cgcttgctcc
     8701 ccctcttcag ataaagaaac agctacaact gccaattcga cgacttcatc caatagtact
     8761 catacagtca cagatacact tggacataaa gtagagattc caaacgcgcc caaacggatc
     8821 atcggcagtt acttagaaga ctatctgatt gcactagggg aaaaacctat cgcccagtgg
     8881 actgttggca gtggttccat tcaagattat ctgcaagatg atttaaagga tgtcccaacg
     8941 atttcttatg accttcctta tgagaaagtg ttaagctttg agcctgattt gctgttgatt
     9001 tcttcttccg ctactgtcga aggcgggaaa tacgaacaat atagcaaaat cgcgccaact
     9061 tatgttgtaa aaaacggaac agatgtcaca tgggaagaac aactaaaaga tgtcggcaaa
     9121 gctttggcaa aagaaaaaga ggcagaaaaa atcatttccg attatcaaaa atccatcaag
     9181 aaaactcgag aagaactagc agataaaatc gagaacaaga cagctgcagt gttgtgggtg
     9241 accaacaact cagcctttat ggtcagcaaa aatcggtcaa gtggaagaat cgtatacgac
     9301 gatctggaat ttggtgttcc tgatctagtg gaagaggtct ccaaaaaagc cacttctgac
     9361 tggtctgctg tttcctctga aaagctagct gaattagatg ctgactacat catccttgtg
     9421 aacagtgaca aagatgctgc catgttcaat gaaccaagct ggaagaatct aaaagcagtg
     9481 acagacaatc atgtactgca attcggacct gaatctagtt ggctatataa cggacctatt
     9541 gccagtcagg aaatggtcaa tgatatcaaa gaaaaactaa attgatttta actaaaaaaa
     9601 gcatcttcac gattagtcgt gagatgcttt ttttcttatt aacaaactag acgcatagat
     9661 tttattcttc ttttgaagca accctatttt ttactctttc ccatttttga aaacgatggg
     9721 catatagatt ttcttgatcc gttatcccct catactcctc tataagggcg aagtctgaaa
     9781 aatcaatatt cccaatatac gtatcgcctt caaacgtgct atcgatcaat gttcgccaga
     9841 tccgaccagt gtaaggaaga agttcttgaa aaatccgact accacccgaa acatagatat
     9901 cttctgagat cgtttgacta tacgacaaaa gttcatcgat cgaatgcatt acttccgcat
     9961 tttctttcgt gcgaaaattt ggttgcgttg tcaacacgat gatatggcga taaggaagtg
    10021 aaagatcccc cattccttca taggtctttc ttcccatcac taaaattcga tccattgtat
    10081 gttcgcgaaa gaaacgcata tcatttggca atcgccatgg taacaaacca tttttgccga
    10141 tcagaccatt tttatcttgt gcccacatgg aaataaacat attatccgct ccttatgatt
    10201 ttagatgttt tgagataaaa aaagtctcaa ttctttgacc agcaaggaat tgagaacttt
    10261 aaaaacatta ttttgtttca cggtgcaaag taactttgcg ttcgcgtggg caatattttt
    10321 gtttttccaa acgatcagga ttgttacgtt tgtttttgct tgttagatag ttacgttctt
    10381 tacaagaagt acactctaaa gtgatgttta cgcgcatgtt tttccctccc atttttacaa
    10441 gaatcctcta agatgactct cagacttgat tatcttacca tgattttttc tagattgcta
    10501 gtgtttttca cgattttgtt aagtaaaact acttgacgat aaatacaacg gttttacttc
    10561 tcaaacaaaa tctttttctt tttgtataaa tacccttaca cttgtttata tagattatgt
    10621 tttttaataa agggggatac taatatgcca tggaacatga aggactttcc tgcatcaatg
    10681 aaaaacttag ataaattgac aagaaaaaaa gcaatcgaca tcgcaaatgc attattagat
    10741 gaagggtacc ctgataaccg tgcgatccca attgccattg atcaagcaaa aaaatggtat
    10801 gaagatgcta gtgaatcaga aaaacgaact ttcgaaaaag aaaaaaatcc aagcaagacg
    10861 gatgaacacg atacgaatcc tcgtgctgga aaactgcttg attcagatgt gatcgtagaa
    10921 tatgaggaag aacaatggat cgtgaaatct aaaggggcaa aaaaggcaag caaccatttt
    10981 gatacaaaaa aagaagcaat cgaaaaagga aagcaagtag cacaaaacaa agaatcaact
    11041 ttgttcattt ataaaaaaga tgggacaaaa gaaaaagaaa tcgattattg aacatatgta
    11101 aaggggttgt gacgaaaatc gtgtcacaac ccccttatgt tcgaaacaat ggtgttcctc
    11161 tgccaactcc gaaccataag tcataatatc tataaaacgg gaagagctgt tttttgaaat
    11221 tgtttcttat cactcagtgt taaacggtag gagaaaacct cttttttatt gatatttgat
    11281 tttcatcaaa cagtcacata gtcgattgct tctgcttgcg gaatcgtttc atctacaaag
    11341 tagactttat accatgtcaa gaaactgaag attgtccaga tttttctttg ctcttcttta
    11401 cgtccttcgt gatggtcatc tagcaacttc atgatttttt cttgatcaaa aaattctttt
    11461 gcaaaatctg cttcgaataa tgctttgact tgttcgtagc cgtgttcttc tttcaaccaa
    11521 gctttgattg gcacagggaa tcctaatttc acacgattcg accattcttc cggaagatgt
    11581 ttgttcgcag cttgtctgaa aatatctttt gtgttgtttt gattcaataa aaacttactt
    11641 ggtatttgtt gagccaattt catgacttcg atatccaata aaggtactcg gacttccaaa
    11701 gaacttgcca tagaaagttt atctgccttt aacaagatat cttttggcat ccattgatga
    11761 agatcgacat attgcatctt attgacttca tctttgattc cttctgtttt ctcgtaatga
    11821 acagtcatga tttcatccac agatggtgct gtttgatatt ttggtgttaa cacttctgat
    11881 gcttctgatt cttcgaacac tcgagcatga ccgataaaat attcttttgc aggagcaagt
    11941 gattcatata aatggatccg tccatggaag tttttcattt ttccgatttt acgtcctaaa
    12001 ttatagcgtg ttccttttgg taattttttc aatccttgag caatcacacg gatgaatttt
    12061 gaattcgtat ggaatccata cgtttggtat cccgcaaaca gttcatccgc cccttctcca
    12121 gattggacta cccgcacact ttgtgaagca agatttgcta aaaagtacaa aggtacgcaa
    12181 gaaggatttg aatcaggttc atctaagtga tactggatca aagggaatgc tttgaacgac
    12241 atgtctccgt cgatcactgc tgctgtatta tctaagtcca atagttcggt tagttttctg
    12301 gcttcaatcg cttcattata ggttttatca tcaaacccga ttgagaaaga atgatctggt
    12361 ttcaatacag aggttacata actggaatca accccgctgg ataagaaaga accaacttct
    12421 acatcactga ccgtatgtgc ttcaatagaa gcttgaacgg tttcgtcgat tttttcgatc
    12481 cattcttgtc gggaatgcgt ttcttttgtt tcaaaatctg catcccaata ttgctgaatg
    12541 tctatttttc catctttata cgtataataa tggccttcag gcaaacgata gacatctttg
    12601 aagaatgttt cgccgtttaa tggagaatat tggaaagtca tataaggttt caacgcttct
    12661 ttgttcaatt ctttgttaaa atcaggatga ggcagaaaac ttttgatttc tgaaccaaac
    12721 ataaatgttc cgttcatttc tgcataataa tatggtttga tcccaaagtg gtctcttgca
    12781 ccgaataact cattcttttc attatcccaa atagcaaaag caaacattcc gcggatcttt
    12841 tgcaataatt ctgttcccca ttcttcgtaa ccatgcaaaa ggacttctgt atctgcatgt
    12901 gtttgaaaaa cgtggccagc tgcaatcaat tcttctctca aaggctggta attatagatt
    12961 tcgccattaa aaataatgat ctttgtccga tcttcattgt aaataggctg agtaccgccc
    13021 tctaagtcaa taatacttaa tcttctgaat cctaaagcaa catgtttatc gatatattct
    13081 cctgaactat taggtccacg gtgaacgatt cgatccatca ttgtattaat aattgttttt
    13141 ttgttgtcct tgtcgttaac aaaacctaca ataccgcaca tttaactttc acatcctaat
    13201 ataaaattac tgtttatttc attcatatgc atcttatcat agaataaata gcggacgaat
    13261 attttctctc ttacaatttc gtcacgtgaa aacaaaagat gaaagtttct gtttttacag
    13321 aaaataaagt cccaatatct aatttaccat gttttctttt ttgaggctac cttattctct
    13381 tcgtgttaaa aaaatattag tggatgctat ttccaaaaaa accagcaaaa aaagacacga
    13441 cctaaagatc gtgt
//
LOCUS       DARDHA010000072        13231 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000072 DARDHA010000000
VERSION     DARDHA010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.072"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            115..1854
                     /gene="yfcC"
                     /locus_tag="SDA10_001643"
     CDS             115..1854
                     /gene="yfcC"
                     /locus_tag="SDA10_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEG4240550.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2011..3195
                     /gene="iadA"
                     /locus_tag="SDA10_001644"
     CDS             2011..3195
                     /gene="iadA"
                     /locus_tag="SDA10_001644"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEG4240551.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3306..3509
                     /locus_tag="SDA10_001645"
     CDS             3306..3509
                     /locus_tag="SDA10_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEG4240552.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3610..4272)
                     /locus_tag="SDA10_001646"
     CDS             complement(3610..4272)
                     /locus_tag="SDA10_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4240553.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4285..5787)
                     /locus_tag="SDA10_001647"
     CDS             complement(4285..5787)
                     /locus_tag="SDA10_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4240554.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6335..8014
                     /locus_tag="SDA10_001648"
     CDS             6335..8014
                     /locus_tag="SDA10_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEG4240555.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8141..8620
                     /locus_tag="SDA10_001649"
     CDS             8141..8620
                     /locus_tag="SDA10_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEG4240556.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8839..9519
                     /locus_tag="SDA10_001650"
     CDS             8839..9519
                     /locus_tag="SDA10_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEG4240557.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9527..10858
                     /locus_tag="SDA10_001651"
     CDS             9527..10858
                     /locus_tag="SDA10_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG4240558.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10873..11835
                     /locus_tag="SDA10_001652"
     CDS             10873..11835
                     /locus_tag="SDA10_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEG4240559.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11946..12284
                     /locus_tag="SDA10_001653"
     CDS             11946..12284
                     /locus_tag="SDA10_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEG4240560.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12363..13070
                     /locus_tag="SDA10_001654"
     CDS             12363..13070
                     /locus_tag="SDA10_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEG4240561.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4200 a         2335 c         2903 g         3793 t
ORIGIN      
        1 aagtgatttt ctaataaatt attcatgtgg aaatgtcatt tttactgtat aattcatttg
       61 attatccatt ttttattcat caaaattact gaaaaaagaa aggaagaaat aaatttgaag
      121 aaaacaaatt cttcaggaaa accgaaacgc gacttcacta aattatcgac accccatacg
      181 tatgtcatta tttttggtgt agtcatcttt gcatggatac tgacttttgt cgttccagca
      241 ggaaagttca gcacgcaaga tattgaatac aaagatgcaa atggcgaaac aagtactcgt
      301 actgttttac gtcaagattc cttccgttat gcttacgagc tggataagtc gtatgtgttt
      361 gatcaattag aagaactgca agatcatcca gcagaacgtg aaaaactgga tgttccggaa
      421 aaaggattgg aaaaagtcat tgctgacgga gaaaaaaatc ttacgcagga aaaactagat
      481 gaaatctcct tgacagatga tgtgctttac gatcagtacg gcgaaaatat ctatgatact
      541 tctaaaaaat tgcataaaac agccaaaata tggggaacag atgattttgg cggctttggt
      601 ttcttaaatt ttgtttttga aggactagta tctggagata agtatggatc tgctgtaggg
      661 atcgctgcat tgatccttgt tgttggaggt gcttttggta ttattatgcg taccggagcg
      721 atcgacgcag gtatctatgc gtttatcagt aaaactaagg gactggagcg tttagcttta
      781 ccgctattat tctttgcttt ttcattcggt ggcgctacat tcgggatggc agaagaggta
      841 attccattct caatggtgat ggttccattt gtcattgcgt taggatatga ctcgatcgta
      901 gcagtaaccg tgacttatgt cgcatctcaa gtcggaaatg cgacctcttg gatgagcccg
      961 tttagcgtag cggtggctca agggattgct ggaatccccg tgttgtctgg tgcgacattc
     1021 cgattgatta tgtgggtagt tgttactgca ttagccgctg gttatatgat gatatatgca
     1081 gaaaaaatcc gtaaaaaacc agaaagttcg ttgacttaca aatcagatga ttatttccgt
     1141 tctcatatca aaaagacatc tgatgaaaat aaaaaattca tgcttgggca taaattgatc
     1201 ttattagaga tgttagttgt ccttgtctgg atcgtttggg gcgtgactca aaaaggctat
     1261 tacattccag aaatcgcttc tcaattcttt gtgatgggat tagttgctgg aatcattgct
     1321 gttcttttca aattagatgg aatgaaagtc aatgacatcg cttcttcttt ccaatctggt
     1381 gcagctgatt tagctggtac agcaatcgta gtaggtatgg ccaaagggat tcttcttgtc
     1441 ttgggaggat ctgatgcatc tgttccttca gctttgaata cgattttgca tgggatcggg
     1501 acggcgttga ccggtgttcc agctgtcatt ggtgcttggg cgatgtacat cttccaaagt
     1561 ttgttcaact tggttgttac atcaaattca ggacaagcag ctttgactat gccaatcatg
     1621 gctccactgg cagatttagt aggtgtatcc agacaagtag cagttcttgc ttatcagctt
     1681 ggttcaggtt ttatggacgc ttttacaccc gtatcagcca gcttgattgg tgtgctaggt
     1741 gttgcacgga tcgaatgggc aaaatgggca agattccaaa tcaagatgca aggattcctg
     1801 tttgttttag gaactatttt catcatgatt gccattgcaa taggtctgca gtagtactgg
     1861 agacctgtaa ataaaaaagt aatccaaaca gtagataagg actccaagaa ccgtcatttt
     1921 acggtaaaat caaaggagta aagatcctat cgtttggatt attttttaag atcctatcat
     1981 cagttatact taaagaaaga ggaaaaatgt atgaaaatca ttcgacaaat tgaagtgttt
     2041 gcaccagatt acttaggaaa aatggatgta ctgattgccg gtgggaagat tttggctgta
     2101 gaagaacagc tagaaggcgg ctacgaagga gtggaagtag aagaactttc aggtgaggga
     2161 aaagtactaa ccccgggttt cattgactgt catttccata ttttaggtgg aggaggagaa
     2221 ggcggttatc aaaatcgaac gccagaagtc acattgagcc aattgactac agctggtgta
     2281 acgacagttg tcggttgttt gggaacagac ggagaaggta gagatatgac tgcgttgatc
     2341 agcaaagcga aaggtctgga agcagaagga atctccactt atgtatacga aggttcgtac
     2401 cgattgccgg taaaaacagt aacagattct atcatcaaag actttttgac aattgataaa
     2461 attatcggaa tcggagaaat cgcagtatcc gatcaccgtt cttctcagcc aagttttgaa
     2521 gagttcgctc gtgcagtagc agatgctcgt gtaggaggca tgttatccgg aaaagctggg
     2581 atcatcaatg tccacttagg cggaggaaaa agaaagctag agctattgac acgaatcgta
     2641 gaagaaacag aaattccgat cacacaattt ttgcctactc atgcaaatcg cacaccagaa
     2701 ttatttgaag cttgcgtggc atttgccaaa cgtggtggca cgattgactt cactgctagc
     2761 gaagatcctg atttttggga gaagacagat ggtgaagttc gcttcagcaa agcactgaaa
     2821 cgattgatcg aggaagatgt ttctttagat aatttcacga tgacctcaga tggacaagga
     2881 agcttgcctt attttgatga aaacaatcat ttcctagggt taggagtagg aagtgccaag
     2941 gcattgctag tagggatcaa agaagctgtg caaaaagaaa gtatcccttt ggaaatcgcc
     3001 ttacgagcta taaccagtaa tccagcacgt atattgaaat tggataaaaa aggaaaaatc
     3061 gaaattggtg ctgatgcaga tctgtgtatc ttggataaag aaacgcttga tattgataca
     3121 gttatcgcta aaggagaaat aatggtccaa gaaaaagaag tcaaagtatg ggggactttc
     3181 gaaaaaagtt tttaatcaac agctctaact atctttttta gaaaacgagc gttctgtttg
     3241 tctgtatctg ttgttgatgt taccctgaag gtagatcaat aaacaaacta agaggaggaa
     3301 gcacaatggc agatttaaaa ggacgttcta aagatatgaa agataaagta gtcggtgaag
     3361 gaaaagaagc atacggcaaa gcaacagacg acaagagcaa agaaacagaa gggaaagcac
     3421 aatcactagg tgctgacatc aaagaaaaag ctcgaaattt aggcgacgaa gtcaaagaca
     3481 agttttctcg aaaagacgaa aaaaaataaa aaccacttat tactaattga atcaagcaaa
     3541 taaaaaatcc gaaccatatc tgcgagacag atatggttcg gattttttat ttgatcatta
     3601 tcctgttttt taaacttcga ttttttgaaa ttttttattt tttagttcaa agacttcatc
     3661 acagcttttt gctacttcaa tctcatgagt cacgatgata atacagcgat tttgttcatg
     3721 ggcgatttct tggaaaagtt tgacgatatc ttgtgaagtt gtttcatcta gatttccagt
     3781 aggctcatcc gctacgatta gttgatgttc acagcagata gcacgaacga tagcgactcg
     3841 ctgctgctga cctcccgaaa gtttggtcac ctgttttttt gctaatattt catcaattcc
     3901 tacacgagcc agtcgatcta aagcaacttg tcgataattc tcttgcttgg tcttagtgat
     3961 ttccattgct gctatgacgt tgtctagagc cgataaataa ggaagcaagt tgtaagcttg
     4021 gaaaataatc gagacatcat ttttacgata acttcttaac ccgattttgt tcagtgaacg
     4081 atcttgatag tatacctttc cttcttttgg tgcatctagt ccagaaataa gcgaaagaaa
     4141 agtcgttttt ccagaaccac ttgctcctaa aatagcgtac atttttccag cttcgaaatt
     4201 cagttctaca tctttataaa gcgcatcttg cggatcttga taccaaaatc ccaattgttc
     4261 tgtccgtaac atcaaattcc tcctttagtt taataggatt tttcttgggt ttaatcttaa
     4321 tatccctata gaagctaaaa agacagaaaa cagactgatt ccaaatgcta ctccggctaa
     4381 tagtcccaat tgtgcaggtt tgacattgat ttccaattcg ctgacttgat cagaaacagc
     4441 aaatggatta ttcgtggttc cttgttgacc atcgcgttga ccgccttgtt cttgtctgtt
     4501 acctggcatt tgtccaccgc caggagcttg ctggttttgt gtcgtggttt ctgtctgttg
     4561 gtcaagtagc tgctgtccca cggcattcgc gacgacgttt ccgctaagcg aagcaactcc
     4621 taatgcaaga atcatacaaa tagccacttc ggtaaacaat tggagaatga ttttacttct
     4681 ggattctcct aaagatagca agacgcctaa ctcatgccga cgctcccgaa tggtaatcat
     4741 aatgatcaga gtaagtatga taataccggc tacagccact agtaaaacaa catttttaga
     4801 aaaacttgcc acgttgttta aaggttccaa cattgactga tacatggaat catttgattg
     4861 taagctaaat gtatctgtgt cgatcagctt ctcagcttct ttgagaaact catccatttt
     4921 atctggatca gatagagtgt aaatagcaga atctatcgtg ttatcgtcag atgtcccatt
     4981 tagttcattt gcaaaagtgt aagaagtgta aagtgtattc gacggattca tgaagttgaa
     5041 tttcataccc atactgccgc ttgtttcgct ggattcataa attcctttga tcgtcatttc
     5101 ataagtggta tcttcatctt cagtactcgt gattgtgaaa gtatctccga ctgccaggtc
     5161 atttgtttct gctagagtag aatcaatgac tacattgttt gtgcctttgt cgctgtcatc
     5221 tattccttca ccatccgtga tttttgctgt tccatcagaa aaattactat tttgtgcagt
     5281 tgctgacaca ccactgattt gaaagtctgc ttgagtcatt tgcggattcc cgcccattcc
     5341 tccagcaggt tcaccgcctg catcagtagt ttctgaactg tcttcacttg aatcagaact
     5401 agtgatggca gttattccat cttttgccat agctgaggcg gaactttcaa aggaataact
     5461 ctttacatta tctaattcag ctattttttc cgcgtccgtt aaggaaactg gtgtaagaga
     5521 aaaactgcct ggatctggac gttcttcagt ggtagaaccg cttgaagaag tttgttcctg
     5581 ttttttaaat gaagcttctc gattggcact taaagtcact gttgctccaa cttctttttg
     5641 tgcttgattc gctgcttctt ctgcagcgct tcgaatcgtg aggccagcta gcacaaaaat
     5701 aagaatggct gtgaacacgg caaacaataa caatgaacgt ccccatttta cttttgtact
     5761 tttccatgca cgtttcataa aattcatatt ttgcatccct cctagtagat gcatttgata
     5821 atagacaacc tatcttaaag ctagcttaaa tcttatgctg tttgcacacc atcaacatga
     5881 ggttctgtat atataaaaag tcactatagt tattgcaaaa aatgtggtaa aatgaaaaaa
     5941 gtatgtttca aaccgacgaa gaagaaatga aagaggaata gcaaaagaaa tcctagatgc
     6001 tacagaggga tttttacgtg catagttgtt ctaacaattg ctgatactag cgatttagtt
     6061 tatttgttgt ataataatgg ggaacagctg aatacttata taagtattct ggctatagcg
     6121 tttcgcaaaa gatccatttt gttaatgaag tatatccaaa ataaaatgcc catccaactg
     6181 tcagaacgcg gatgtgctgg taattttcct gctgattctc cataagttga gaaatagtga
     6241 ttttgcatat gcttcattgg aatggctcat atatttagat aaagaagaaa atcgacatct
     6301 ttaaacttgg atgtcataag aagaggtgaa gtttgtgagt accattaaaa ttatcccatt
     6361 aggcggcgtt cgtgaaaatg gaaaaaacat gtatgtcgca gaagtgggcg aggatatttt
     6421 tattttagat tgtgggctaa aatatcctga aaatgagttg ttggggatcg acgtcgtaat
     6481 ccctgatttt acgtatttag aagaaaacat tgatcgtgta gctggtgtct ttttaacaca
     6541 tggacatgca gatgcgatcg gggcacttcc ttacttgttg tcaaaaattt ctgtaccagt
     6601 attcgggaca gagttgacga tcgagttagc aaaattgaat gtcagtcgta atgaagcagc
     6661 caaaggattt aaggatttcc atgtggtaga tgagcataca gaaatagact ttggtgaaac
     6721 agtagtgagc tttttccgta ccactcatac gataccagat tcagtgggga tcaatctgaa
     6781 aacaaaagaa ggtagtatcg tttatacggg agattttaaa tttgaccaaa cggctatccc
     6841 aatgtatcaa acagattatg cacgattggc ggaaatcggc aaagaaggag tcttagcctt
     6901 actaagtgaa tcggcaaatg cagaaaatcc tcagcctgta gcttctgaag cccaaatagc
     6961 agatgaagta tttgatacga tcaaatattg ggaagggcgt atcatcgtcg cttgtgtagc
     7021 aagtaatttg caacgagtgc aacaagtatt ggatgcagca gaaaaagcac aacgaaaagt
     7081 tgtcttgact ggtcaagatt tcgaacgaat tattcgcaca gcgatgcgtt tagaaaaact
     7141 tcaattacca agtgaagatc tgcttgttac gttgaaagac atgaaaaact atgatccaga
     7201 agaactgatc attttagaaa ctggccgaat gggtgaaccc atcaagtctc tgcaaaaaat
     7261 ggcaaacggt acacaccgta atgtcaagat tgaagaagga gatttggttt atatcactac
     7321 tacgccaagc atcgcgatgg aaaccatcgt ggcgaaaacg gaagatatca tttatcgagc
     7381 tggtggaaca gtgaagttga tctctgagaa tatgcgcgtt tctggccatg ccaatcctaa
     7441 tgacttgcaa ttaatgatca atttgatgaa accaacatat tttgtgccag ttcaaggaga
     7501 gtatcgtgaa ttagctgctc atgcagattt ggctcatgca gtagggatgc catacaaaaa
     7561 tatctatatt actggacgcg gagacgtgct ggaatataag aacaagcgta tgagtgttgc
     7621 aggaactaca agtgcagaaa atgtaatgat tgatggatta ggcataggtg atatcggaaa
     7681 cattgtttta cgtgatcgaa aaatcttgtc agaagacggg attttcgtag cggttgtcac
     7741 tatcagtcgg cgagaaaaga aaatcatttc aaaacctcaa attacttcac ggggattcgt
     7801 ttacgtaaaa gctagccgtg atctgatgaa agaaagcagc aatatgattg aaacaatcgt
     7861 tgagaaacat ttagaaagca atgattttga gtggagcaaa ttaaagcaag acatccgaga
     7921 tcaattaggc cgttacttat ttgaacaaac aaaacgccgc ccagtgatct tgccagtcat
     7981 tatggaagct actcaacgtc gaaataaaaa ataaacaaaa agagtaagat gaacctctct
     8041 atagagtgct cgtcttgctc tttttttgcg aaaaacaaat aatttttgaa tacagcatcc
     8101 ctcgctttat aataaacgat agaataaagg gagtgaatca atgatgagtc aaacaaaagt
     8161 cgaacgttat ttagaagaac aaaagatcag ctatcaacca cttgatttta gtcatgtggt
     8221 agaagaatct ttcacagaag aactacaaaa aagagggatt gatccagctt tgatatgtaa
     8281 aactctcgta gtgaaaggaa acaaaactgg ggtgatcatc gcattgattc ctttgaacga
     8341 tcacttagat tacaaaaaaa cgagacaagt gacgaaagat cgtaaagttg gttttcctgg
     8401 aatggatttt gtattaatgc atacaggata tccccacgga gcaaacaccc ctattggaat
     8461 aaaactcgca catcctgatt attgtttttt agcagacagt tctttaaaag aaaaagaaga
     8521 agtaattgtg tcaagcggtg aaatcgggag aagccttcgg ataaaagtag ccgatttaga
     8581 aaagatcgtc catcctattt acgctgattt aattagttaa gggacgagaa gagaagctaa
     8641 gcagagcaga gacctcaaac ttgagattat atctgcttgg tacttggtgc atcctgtatt
     8701 atctgttaca attgctttgt acatactgta ctatcaatcc aatgaaaata tatttagcag
     8761 ctatattttt tcattgatgt gcaagtgatg aaatcgtttt taataaaaaa gatacagaga
     8821 aaacgcgagg tgtaaaatat gaaaattacg tatcatggac attcctgttt aacaattgaa
     8881 atggaaaatg gccaacgcgt gatgattgac ccgtttatta caggaaatcc attgacagac
     8941 ttaactgcag aagaagtaaa gacagattgg ttgctgatta ctcatggtca tagtgaccac
     9001 ataggagata tgattcctat tgcaaaagaa aatcaagcca caattatcag tattgttgag
     9061 attgctgatt atgcgaacag cagaggagta gattcattcg gtatgaatat cggtggaaaa
     9121 catgcgtttc cattcggcgt ggtaaaattc gtacatgcac agcatagttc aagctatgaa
     9181 gtagacggta tcgttcagta tatgggcgag cctagcggaa ttattattca agcagaagga
     9241 aaaacgattt atcatgcagg ggatactgct tatttcagtg atctaggatt gcttgcagaa
     9301 gaattcgata ttgatgtcgc tttcttgccg attggcgata actatacgat ggggccagaa
     9361 gatgcagcaa aggctgcgca aacaattcgt gccaagaaag tagttccaat ccattacaat
     9421 actttccctg tcattgaaca agatcccgaa tcttttgtag agttactgga agatagtgaa
     9481 ggaaaaataa tgagcgtctc ggaaacgctg gaagtgtagg cagaaaatgg cgacaaaaca
     9541 tgatttgatt ttagaatata ttgaaagttt acctgttggc aatcgaattt ctgtaagaag
     9601 catcgcaaaa gaattgaatg tcagtgaagg aacagcgtac cgtgcaatca aagatgcaga
     9661 aaatgtcgga ctagtttcaa caattcaacg agtagggacc atccgtatcg aacgcaaatt
     9721 aaaaaaacat attgaacgtt tgacatttgg cgaggtcgtc aggatcattg aaggggacgt
     9781 tttaggcggg gcggcaggat tagacaaggt cttgaataaa ttcgtcatcg gcgcgatgac
     9841 taaacatgcc atggaacgct atattacgcc aggatcacta atgatcgtcg gcaaccgggc
     9901 ggaagtccaa aaattagcat tggaagatgg cgccgcagta ttgatcacag gaggattcaa
     9961 tacgactgct gagattgcaa agctagctga tgatcttgaa atgccgatct tacgcacctc
    10021 ttatgataca tttacagtcg caaccatgat caaccgagcg ttaagcgacc aattgatcaa
    10081 aaaagatatc atgcttgtca gtgatatcta tatgccagct gaaaagactc attatttgca
    10141 gcagacagat acgatttctg attatcagcg tttgtcagaa gaaacgcagc attcgcgttt
    10201 tccagtcgtc aaccgccatc atcgtttgat ggggattgtt acggcaaaag atgttttagg
    10261 gaaatcgcca aaccagatca tcgatcgggt gatgacgaaa gaaccaatca gtgtaaagaa
    10321 aacaatgagt attgcctctg ttagccatca aatgatctgg gatgggctag aagtcatgcc
    10381 ggtcgtttcc gatgacttga cattggaagg cttgattacc cggcaagatg taatgaaagc
    10441 gatgcagctt gtccagcgac aaccgcagat tgcagatacg atttcggatc agatatctgg
    10501 aaatatccat acgattgata ctgaccgtga aggaaatcgt ttggaaaacc ctaagttcaa
    10561 attcgtagta gcaccacaaa tggtcaatgg cgttggaacc atttcttttg gcgtacttag
    10621 cgaaatcatt tctgatgtcg cacaaaaaac aatggtgatg aaccaaaaac gaaatatctt
    10681 gattgaacaa gtgaacctac attatttgcg gctgatccaa ttggaaagcg aattagacat
    10741 tcgtccacgt gtattggaaa tcggcagacg ttccgctaaa ctggatatcg aagtgtttat
    10801 tgaaaacacc atcgtttcca aagcaatcgt tgtttgtcaa gtaatggaac gttcttagaa
    10861 agcaggagtg aaatgacagt acagcaagaa atagtagata caataaaaaa atacgataaa
    10921 atcattatcc acaggcattt gcgccctgat cctgatgcat taggatcaca agtaggatta
    10981 gccgaactat tacgggaaag tttcccagaa aaagaaatac gtcaagtagg tgaaacgatt
    11041 gaagggcttc gctttttagc agaaatgcaa gaagttgaag atgtcttcta ccaaggagcg
    11101 ctagtcatcg tgactgatac agctaattca cccagaatca gtgatcaaag atatcagttg
    11161 gcagaaaaac tgatcaaaat cgaccatcat cccaatgatg acccttatgg agatcttgtg
    11221 tgggtaaata caaaagcaag tagctgtagt gagatgattg cagaatttgc tttgatgttt
    11281 cctgaatctt taaagatgaa cgttaaggct gcacggttac tttatgcagg aatcgtcgga
    11341 gatacagggc gctttttgta tccctccacg acgtcccgta cattagagat tgcttcgata
    11401 ttgagaaatt atccatttga tgcaagtgct ttgaacagag agattgaaca aatgccgatg
    11461 aaagtcgcta aactttccgg ttatctttat caaaatatcc aggtggatga aaatggtgca
    11521 ggaagaataa tccttccgca agaaattctt gataaatatg gaatcgatga ttcagagaca
    11581 gcttcgatcg tttcacttcc tggagtgatt gacgaagtgc tggcttgggg gatttttgtt
    11641 cagcagcctt cagggtatta ccgcgtacgt ttacgttcta aggggccgat catcaatgaa
    11701 ttggctaaac gccatcatgg aggaggccat ccattagcaa gtggtgcgaa tgcaaaagat
    11761 ttagcagaag tagaggaaat ctataaagag atccaagata tatgccaatc gtataagagc
    11821 agaaaaacag aatgatactt cgttcgaaaa cgtgcctttt gtagtaaaaa tcacaaatgt
    11881 atcttttcaa gatcttcttt ttccgttaca atagtgctca ctaaagattt taaaggagaa
    11941 agaagatgac attaccaaat tgcccagaat gtggctcaga gtacacatat gaagatcgag
    12001 gcttgttgat ttgcccagaa tgcgggaacg aatggaacaa agaagaacaa gaaactgtca
    12061 tagaagacgg attagtggta ttagatgcaa atggaaaccg tctgttagag ggcgatagcg
    12121 tgacagtgat taaagacttg aaagtaaaag gtgcgtcagg tgccattaaa caaggaacaa
    12181 aagtcaagaa catccgttta gtagaaggcg atcataatat cgattgcaag gtagaaggat
    12241 ttggaccgat gaaactgaaa tcggaatttg tgaaaaagaa ttaaagaata ttcaaggtct
    12301 tttcacattc ttttcctact actaatgata aaataaagta aacgaaaagg agtgaaacga
    12361 aaatgtatgg ttatggaatg tatggttttt tagatccgac atttcttttg gtcattcttg
    12421 gattagttat ttccggaatt gcgtcagcgt acgtcaatag tactttccga aaatacgatc
    12481 aagtgagaag caaaaacaat gtgactggta cacaagcagc acaatatata ttacaaagtc
    12541 aacagataaa taatgtcggt gttcaacaaa ttgccggaga tctcacagat aactacaatt
    12601 caggtaataa gatgttgagt ttgtctcaag caactgcaca gtctacttca gttgctgcga
    12661 taggtgtagc agctcatgaa tgcggacatg cggtacaaga tgcttcgggt tatgtcccat
    12721 tgaagattcg tgcagcactt gttccagcag ctaactttgg ttcgatgatt tcgttcccct
    12781 tgattatggt aggtgtattg cttagctgga actctacgtt aatcaatatc gggattttcg
    12841 ctttttcttt agcattactt ttccaattgg tgacattacc agtcgaattt aacgcgtctc
    12901 ggcgagcaat ccaaattttg agtgaaggcg gactactgac ggaagaagaa gtaccgatgg
    12961 caagacacgt attatttgca gcagcactga cttatgttgc agcagcgctt tcgacattcc
    13021 tgcagctgtt gcgcttgatc ttattgttcg gcggcaaccg aagagattaa aataaagaag
    13081 ttgtgaaatc agcgctttga tctgagctaa taccgagagt tagctgattg aataccattt
    13141 atttggaata agggctcttt gtcaataagg actgatgggt tgtacaaaat caaatctggg
    13201 tcagaatgaa cctcattctg gctcagattt t
//
LOCUS       DARDHA010000073        13197 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000073 DARDHA010000000
VERSION     DARDHA010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.074"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(12..1535)
                     /locus_tag="SDA10_001655"
     CDS             complement(12..1535)
                     /locus_tag="SDA10_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEG4240562.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="SDA10_001656"
     CDS             1787..2968
                     /locus_tag="SDA10_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG4240563.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="SDA10_001657"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="SDA10_001657"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEG4240564.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="SDA10_001658"
     CDS             complement(5053..7764)
                     /locus_tag="SDA10_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4240565.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="SDA10_001659"
     CDS             complement(7774..8475)
                     /locus_tag="SDA10_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4240566.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="SDA10_001660"
     CDS             8661..9260
                     /locus_tag="SDA10_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG4240567.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="SDA10_001661"
     CDS             9341..10354
                     /locus_tag="SDA10_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG4240568.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="SDA10_001662"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="SDA10_001662"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEG4240569.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="SDA10_001663"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="SDA10_001663"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEG4240570.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="SDA10_001664"
     CDS             11802..13175
                     /locus_tag="SDA10_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4240571.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
BASE COUNT         3751 a         2622 c         2448 g         4376 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtat
//
LOCUS       DARDHA010000074        13178 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000074 DARDHA010000000
VERSION     DARDHA010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.075"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            133..1274
                     /locus_tag="SDA10_001665"
                     /pseudo
     CDS             133..1274
                     /locus_tag="SDA10_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1400..2479)
                     /locus_tag="SDA10_001666"
     CDS             complement(1400..2479)
                     /locus_tag="SDA10_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG4240572.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2582..3022)
                     /locus_tag="SDA10_001667"
     CDS             complement(2582..3022)
                     /locus_tag="SDA10_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG4240573.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3079..3489)
                     /locus_tag="SDA10_001668"
     CDS             complement(3079..3489)
                     /locus_tag="SDA10_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG4240574.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3669..4103)
                     /locus_tag="SDA10_001669"
     CDS             complement(3669..4103)
                     /locus_tag="SDA10_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG4240575.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4203..5051)
                     /locus_tag="SDA10_001670"
     CDS             complement(4203..5051)
                     /locus_tag="SDA10_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG4240576.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            5440..7206
                     /locus_tag="SDA10_001671"
     CDS             5440..7206
                     /locus_tag="SDA10_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEG4240577.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7308..8567
                     /locus_tag="SDA10_001672"
     CDS             7308..8567
                     /locus_tag="SDA10_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG4240578.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            8714..10015
                     /locus_tag="SDA10_001673"
     CDS             8714..10015
                     /locus_tag="SDA10_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4240579.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            10018..10869
                     /locus_tag="SDA10_001674"
     CDS             10018..10869
                     /locus_tag="SDA10_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4240580.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            10882..11697
                     /locus_tag="SDA10_001675"
     CDS             10882..11697
                     /locus_tag="SDA10_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HEG4240581.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            11848..13014
                     /locus_tag="SDA10_001676"
     CDS             11848..13014
                     /locus_tag="SDA10_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG4240582.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
BASE COUNT         4502 a         2363 c         2446 g         3867 t
ORIGIN      
        1 ttacttaaat tgttaaaact tgaaaatatt actgtttttt tcataaattt agacaaaaaa
       61 acagtaaaca tataggtaga atctctacta tcttatttct tgcatacaaa aaaatcaagg
      121 agtgagagag ttatgagata ttcaaccatc ttaatatcaa catttgcagc cttaagctta
      181 tcgcttacta taaatcagac agtccacaca gaggagacag aaacagccaa atggacagaa
      241 accaagtggg ccaacgtcaa tcgaattacc tttcaggatg aattttcatc cttcaattca
      301 ggagtcgcaa aattaagaaa aggacaaaca gcgaccatca aagcaaaaat tgactattcg
      361 ggagacttag cacctatact acaatcagct gttacatttg agaatgttga tcccactttg
      421 tcgattggct atgacgagaa cactttgact atccaacaca agcaagcgat atttaccttc
      481 accgttacat taaatcagga cttgttaaaa ccagcgcttt ttactgtcaa ggtatcagat
      541 ggactaccga acgatcatca tttaacgccg tatagtcagc ggcagacaat tgagcaagat
      601 tcagccatcg actcaggagg agatcttgtt gaagaaccaa cagataaacc agaaaatgaa
      661 aataagccag aagtaccgcc tacagaaaat ccagacggag aacagaaacc ggaaatagaa
      721 cctggagaag aacccgatac agaaactcaa ccagagccag acaataaatc aaaacctgaa
      781 ataacccctg gagaaaaacc agatgtaact ccagaaccag atacggattc tggaaaccaa
      841 acagtaccgg aaacaaatcc agacacagat aatgaaactg aaaatccaga aaaaccagag
      901 gtagatccag aagaaaaacc agatgtgact ccagaaccag atacggacgc aagagaccaa
      961 ggaataccag aaaaaataaa caaaaaaaca attcaagagg atggaaaaaa agagagtaaa
     1021 aaatcaaatc tagcaattct aaaaataaat gaagagcagc tgaataaaaa atcacgaata
     1081 tttgatagcg cccaatcagc tgaaacctta aaaagtagca aggatacaac ctttgctgtc
     1141 ctgaaacgaa aaataaacag ctaccaaaat ccggagagtc acaaaacaaa gttatactgt
     1201 ggagcggaat cattctatta agtatagcaa caatgctttc tgcaaaaaga tttaaacaaa
     1261 acagatctct ctaacatttg aataatgtct gtgaacgcat ttttcaaata aaaagaaata
     1321 tccagaaaat gaccccaaaa taccagattg aaatctgata tttggggtcg ttcatacttt
     1381 atctttcttc atatttattt taagggatat ctgtttcttt tggaagatgg ttcgggatct
     1441 ttttcacttt caatacatct aaaaagaagg taaagatcgg aatgccgaca atcaagcccc
     1501 aaacgccaaa gaaccgttcg ctaacaagta agaccacaaa tgtataaaaa atcggcagtt
     1561 ctgttttgct ggacatcaac ttcggattca gcacataaga ctcaattaga tggacaatca
     1621 aaatcgtaat caggatataa acaacatctc ggatgcctcc ttgggaataa gctatgaaag
     1681 atagaggaat acaagaaatg ataacacctg ctactgggat cagacttaaa atgaaaatca
     1741 tgatcgctaa actcagcagc tgatgaaagc caaatacagc caaggcgatt gtagtgagca
     1801 acgtatttaa aagagcgaca acaaattgag cttctaatac caaaccaaat gtattgacaa
     1861 acttatcggc aaaatagtaa atatcttgaa agaaccaaga aaattcactt tttaaaaaca
     1921 atcttgagaa tacaattgtt tttttctttt caatcataaa aaagaaactt aagatgaatg
     1981 acatagcaaa cgccactgcc atttttccta tatcctgtaa atagccaagc gcaatcgaag
     2041 cgccattttg aagctgttca aaaaaatcgt ttcgctcaat ataagtatgg atatattgta
     2101 aaacaggatc tgcatctttt ggctgatgct cgtaaaaatt aataacggac tggatcatgt
     2161 caaatgtttg atgaataagc atcgggatat actttgtgaa tgccagatag ataaggtaaa
     2221 cgatcagtgt ataaatcatg atagctaaaa cacctgtagg gattcggaca aatcgctgaa
     2281 tgatttgaac aagatgaatc atcaaataag taaagataaa tgtgagcaaa atggttgtaa
     2341 tcatacttct aactaagaaa agaacaaaaa cgacaaataa taaaacagtg agacggcgta
     2401 ttttttcatt cgccaaaaaa cgctgataaa cggtcaaatc gacaacctcc aaataacaaa
     2461 aaataaacac gttattcatt atatgatgga atatgaaaat aagaaagagc aaaaagaaaa
     2521 accgctaaat gacgttatct tatgagttgg ttttcctcat aaaatcgttc atctagcagt
     2581 ttcagccctt tttctggtct tgatgaaaat tatctagcat ttttcgggtc agatgttgca
     2641 gtgtatccaa ttcttcctgt tcaagtggaa gataagactt gatcttttgt ggaatacctt
     2701 ctgcttgttt ttgcaacgca gcaccttgag cagtcagcgt aacatttacg attctttcat
     2761 cacccgtttc acgctgacga ttaatataat ttttttctgc taactttttt agtaagggag
     2821 tcagcgtacc agaatccaga cacaaatcat ttcctagctc tttcactgaa gtctttttct
     2881 tttcccataa aactaacatc acaagatact gggtataggt aagattcagc ggcttgagga
     2941 ggggagtgta gtgtctaatg atttctttag aggcggcata taaaggaaaa catacttgat
     3001 taactaaata tagatcttcc atttttccac ctccgctgtt ttactatagt gtatcatagc
     3061 ggatttacac aaaaaagtct attcactaac tgctttcaga gtaacttcta tatttccagc
     3121 tgtagctttt gaatacggac acactttatg cgctgcctct aataattctt gcgtttcttc
     3181 cagagaaaga ccttcgatag atccttcaat ctctacacct aagacaaagc tcgtttcact
     3241 ttctgaataa agggaaactt gtgcactgac agtagaagcc ccagagatat ttttttgctg
     3301 tttgatcaat tctaatgcac tgttaaaaca tgcactgaaa ccagctgcaa acaattgttc
     3361 agggttggtt gcattttcga cgcgtttacc aggagctgca atattcaatt cgaatgtacg
     3421 atcgggatta tgcacttctc cagagcgtcc gcctgtgttg atcattttcg tagaatacat
     3481 ttttttcata aaattaccac ctttaattat attttataca attgaatttt acacaatata
     3541 attgtggaaa gcaaagaaat ggattggcat aagataaata agagaaagca catgaaacag
     3601 acgcgctttc tcgaatagca aacatataca aaaaatatca gagacaaaaa ataatctcat
     3661 cataaacatt acatatattt ctcaaaaagt tgttttcttt cttcaaaaat tacttcacga
     3721 tggacctgat gaagcatcca taaataacca aaaggatctc tgaacattga gttagtcaca
     3781 ccaaagtcca tcatttctgt catttcttga atctctacac agccatttcg taatgcttta
     3841 ttatgtgtgt cctcgatatc agagacgatg atattgaacc agatcgtatt tggatgttca
     3901 gcagtaggag cagtcaaacc aagagacgtg ttttcatcca gcatatggaa tctcgtacca
     3961 tacaaggtaa aaatgacttc attattgcct ctagggtaat ctgttgcctc aatcagttgt
     4021 atttcatcaa agattgtttg atacagttct agtgctttca aactatcatt taatacaaaa
     4081 tcgatttccg ttcctttatg cataaagatc tcctctcctt ttcagtcgaa cagttactag
     4141 attcttttat tataccagct aaagaaaacg cattcaaaaa aatagcggat ttacttacca
     4201 actcattcga ttggatgata agtaaatccg cgtcccatta cttctgctac gctgctgaca
     4261 atgacaaagg cacttggatc gatttcttct atcaatggtt tcattcggga aaattcatga
     4321 tcagtcacaa cgatcaataa aacctctttt gactttcgat tatacccgcc ttttgcctct
     4381 agcagggtaa ggccacgtcc aatctcagca cttaagcgaa ggcggatgtc ttccaaataa
     4441 tgttcactca tgaccataac agatttcttt cgattgatac ctgtttcgat ataagtcatg
     4501 acaattgaag tgattgcaat agataaaata gcataaagaa actcttctat tccaaaaata
     4561 aacagattaa gagaaacaac gaatgcgtct gtaaaaaata agccaatcga agtattcaag
     4621 tggaaatatt ttttaaaaat gaggggaggg atcgtcgtac cgccgctaga agagttgttt
     4681 ttatataaaa tcgcaactcc taaagcaaaa atcgcacttc caaaaatgac agacaacaac
     4741 cgatctgaag taatcattgt ttctggaact attgcaatga ttaaaggaaa aaccaagctt
     4801 ccgaaaagta ctttaaaaaa gggtttttta cctaaaaata gaagagctag taccaacata
     4861 acgatattca acactaaaat aactatagct cggtcaaagc ctaaagcagc ttccagtaat
     4921 attcctagac cgctgacacc tccagcagca atatgatgag gccctaaaaa catattgata
     4981 gcgactccta atatgaagag ggcaaaaata atcttaatgt atgtccatat aaattgtctc
     5041 gtttctttca tttattcact tccttaattc attaataata ctcttatctt attataactt
     5101 acttttcacc ttaagttaac tttaattaat aaaaaaatta ggctagtcta aattattatg
     5161 tttcattctt ctagaatata cttctacgcc aaaaactgtt aaaaaacttt tactttagaa
     5221 aattggagaa attgatcgct taagataaat tcaggacact aaatagtaca ggaagtagac
     5281 taaaactttt aaaagtttta agtctatcta tttggcaagt ttaaaaaata aaaattcggt
     5341 atcaaaaaac tgttaccgat aacatttttg aaagcgttgt cttttgcttc tctatcactt
     5401 atagtgatga cgtcatatta aaacacaagg aggaaatcaa tggatacagc tgcaatttat
     5461 catcgaccgg aaagtgaata tgcatttcta tatacgaata caagatttcg tattcggctt
     5521 cgtactagga aaaatgatat caaaaaagta tatgtgctat gtggagatcc atataccata
     5581 acaactgaaa aatggtatca aaaacaacga ccaatgaaaa aatgtctaag taccaatgtt
     5641 catgattatt gggaaattga agtgacgagc gaaacaagtc gcttacagta tgcgttccat
     5701 gttgttgggg aagataacac ggactgtttt tacggtgatc aaggaatttt tccttatcga
     5761 gaaaatgtga ttactgaacc aaatttttat tttcgaatac cttattttca tcaaatcgat
     5821 cgattcacta ttccagaatg ggtaaaagaa acagtatggt atcaaatttt tcccgaaaga
     5881 tttgcaaacg gggacaaagc aaatgacccc gaacacactt taccttgggg aagtaaagat
     5941 ccagaccgag aagacttttt cgggggagat ttgcaaggtg tgatggatca tttggattac
     6001 ttgacggacc ttggcatcaa tggcatctat ttttgtccca tatttaaagc cacatccaat
     6061 cataaatacg atacgattga ttattatgag gtggatcccg cttttggcga taaaaaactt
     6121 ttaaaaaact tgattgatga agcacataaa agagggatac gtatcatgtt cgatgctgta
     6181 ttcaatcata tgggtgtcca ttcaccacag tggcaagacg ttctcaaaaa cggagaaaaa
     6241 tcgatctata aagactggtt ccacatccgt tttttcccag tagattctta tcagatgact
     6301 gacttaccag aaactgctga aaatatccca tatgatactt ttgcttttac tccattcatg
     6361 ccgaaactaa atacagcaaa tccagaagtg caaaaatatt tgctggatat cgcaacttat
     6421 tggataaaag aattcgatat tgatggatgg cgtcttgatg ttgctaatga agtcgatcat
     6481 catttttgga aaaaattcag agaagcagtt acagaaatca aaccagacat ctatatcctt
     6541 ggagagattt ggcattcctc tcaagcttgg ctgcaagggg atgaattcca tgcagtgatg
     6601 aattatgcat ttacagattc gattaaagat tattttgcaa aaaagaaaat cacagcttca
     6661 caaatggtca gtggaatgaa tcatcagcaa atgctttaca gagatcaggt gaacgaagga
     6721 acattcaact tattagattc tcatgatact gccaggatct tgaccctttg ccaaggtaac
     6781 aaagaattga tgaagtctgt gatggccttt atgtttcttc aaaaagggtc tccttgtatc
     6841 tattacggga cagaaatagg gatgactggt gaagatgacc cggattgccg caaatgtatg
     6901 atatgggaga aggaggaaca agatctagag ctattcggtt ttataaaaga acttgtttct
     6961 ctaaggaaac agttgtctaa gattatttct gaaggttcta ctcagtggct aatagtaaat
     7021 gatcgtgaag ataagctcta tttcacacgg aaactagagg gacagatcat ctatgtttac
     7081 tttaatcagt caaaagaacc ttgggtagta gaacaggaaa atgaggtgat tcttagtcaa
     7141 aattgtcagt tgttagaaga cgggaaagca gagatacgac aatacggatt tttgatcatg
     7201 tgttgaaaac tgttaccggt aacaacaaga aagcgtttgc aaatgaagat ttgtgcccat
     7261 tataataagg gtgtaacagc acgttgaata caaaggagga aatgaagatg aaaaaaagaa
     7321 gtgtgaaaca gttgggttta gggttaatct ctttaggaat cttaggcgga ttagcggctt
     7381 gtggcaatgg taactcttca gcagaagggg aagccgatga aggaaaaacg ttaacagttt
     7441 ccgttgattc tggatataaa gattatataa acgaaataaa aaacactttc gaaaaagaaa
     7501 acgatgtgaa aatcaagcta gtagaaaagg acatgttcga tcagttggaa tcattagcgt
     7561 tagatgggcc tgctggaaag ggacctgacg ttatgatggc agcatatgac cggatcggtg
     7621 cgttaggaca gcaaggacat ctggcggaag taaaactggg aaatgaagca gcttacgatg
     7681 aaacagacaa agcccaagtg acttatgatg gaaaaatcta cggagaacct gcagtaatcg
     7741 agacccttgt tttgtactat aacaaagatt tagtcgatac agcaccatca acgtttaaag
     7801 atttggagaa tctatccaaa gacagtcgtt ttgcttttga gtcagaagca ggaaaaaata
     7861 caggattttt agctaaatgg actgactttt attattccta tggtttaatt gcaggttacg
     7921 gtggctatgt atttggtgat gatggcacaa atccttcaga tatcggtttg aataatgcag
     7981 gtgctgtaga aggaatctct tatgcaacag attggttcaa aaatgtttgg ccaaagggaa
     8041 tgcaagacat aaaaagtgca ggagacttcg ccagccagca attcatgtcc aataaaactg
     8101 cagcaatcat tgacggtcca tggcaagcgc aaacatacaa agaaaacaat atcaattatg
     8161 gtgtagccaa aattcctact ctaaataatg gacagcctta tcaaccgttt ggcggaggaa
     8221 aaggatgggt agtcagcaat tatgctaaaa acaaagacct ctctcaaaaa tggcttgatt
     8281 atgtaacgaa tcaggaaaat caagaaaaat tctttgaaat ggtaaatgaa atccctgcaa
     8341 accaacaagc tagggaaaca gcaaaggcta aaaacgatga attaacaaca gctgtgattg
     8401 aacaatatga aacagctcaa gcgatgccaa acatcccaga aatgggcgaa gtttggacag
     8461 gtgcagaaaa cttgatgttc gatgcagcat caggttcaaa aacaccaaaa gaatccgcag
     8521 atgaagcagt aaaaacaatc agtgaagcca tagaacaaaa atactgatta aaatgataac
     8581 cgtccatttt tataaaaaat ggaacaggtg cttcagataa aagtggcaag agcgagatgg
     8641 aaaattatct tgctcttgtt gccattatct taaaagcagt ttgcggttta gcaagtttag
     8701 agggggcatt cagatgcagc aagataaaac aagcaatatc aacgaaaaaa aaatcacgct
     8761 ttgttcactt attccaggga tggggcagtt tgtaaatggc caagctttta aaggtatcgt
     8821 ttttttcgcc atattcgtcc tattcattgg tcagatgctt ctgggcggat ttcatgcatt
     8881 ggtaggattg gttactttgg gaagtatacc aatggaagat cattcattat ttattttgat
     8941 tgaaggcact ttgcaattca tcattacaat cattttcttg attttttacg gtttgaatct
     9001 tttagacgct tatcgagtag cgaagatgcg tcaagagggc aaaaaaatca atcggacgat
     9061 gaaggaagtc atacataata taggtgatga aggttttcct tatctattga cacttcccgc
     9121 ttatcttctc atgattttta cgatcatttt cccagtccta gttacattgt tcactgcatt
     9181 tactaattat gacttcaatc atattccacc agccagttta attgattggg taggggctaa
     9241 aaactttttt agtatcttct ttttaagctc atatcgtaat actttttttg cagtcttttc
     9301 gtggaccgtg atttggactt tatgtgcaac aactttgcaa atcacccttg gtattctcac
     9361 agcggttgtt gctcaccaga aatttattaa attcaaacgt attttcggta tcattttctt
     9421 attaccatgg gcggtcccag cttttattac tatcatgagt ttttcgaata tattcaacga
     9481 tagcgtaggt gccatcaata ctcaagtgat tcctttcata aaccagcttc cttttgtaga
     9541 tatcagtgca atctcttgga aaacggaccc gaactggacg aaagtagcaa ttatcttagt
     9601 gcagggctgg ttaggatttc cctatatcta tgtgatggtt acaggaatct tacaatcgat
     9661 ttcagaagac ttatatgaag cagcaaaaat cgacggagca aatgcatggc agcgcttttc
     9721 aaacatcacg cttcctgctg tcttcctagt ggcagcgcca acatttatta cacagtatac
     9781 ggggaatttc aataatttct ccatgatcta tctgtttaac aatggcggtc ccggaagcgt
     9841 aggcggagga gctggttcta ctgatattct tatttcctgg atctataaac ttacgactgg
     9901 aacttctccg cagtattcta tggcttcagc aatcacgttg attatctccg tcatcgttat
     9961 ctgtgtgtca ttgctggtat ttaaaaagac aaatgcgttc aatatggggg attaacaatg
    10021 aaaaaatttc attcgagtgc tcgattccaa aaacgacttt ctcacttttt tacttacttt
    10081 tttcttttaa tactgtcagt cattatcatc tatccgcttt tgatcacagc aagtactgcc
    10141 ttcaagtcag gaaatattgc agcttttagt ttagatcttt caagtgactg gactttagga
    10201 aactttactc ggctattcag tgaaacactt tacgggactt ggtataaaaa tacattgatc
    10261 atttctgtcc taacaatgat cgtacaagtg actgtagtaa ctttggcggg ctatacttat
    10321 agtcgctatc gttttatcgg ccgcaaatca agtttgatgt tctttttagt gattcagatg
    10381 gtaccgacta tggcagcatt gactgccttt tatgtcatgg ctttcttatt agaagcgctc
    10441 gataagtact ggttcttgac aatgatctat ataggcggtg gaattccaat gaatacttgg
    10501 ctgatgaaag gatactttga cactgtacca atagatttag atgaatctgc taaacttgat
    10561 ggggcagggc atttcaggat ttttttccag attgtattac cgcttgtcaa accgatgctc
    10621 gccgttcaag cattatgggc ttttatggga ccttttgggg atttcatgtt agctaagttc
    10681 ctgcttcgaa cacctgaaaa tcagactgtt gctgtcggct tacaaacatt tatttctgat
    10741 gtgacaaacc aaagagtctc tctttttgct gctggggcaa ttttgattgc actaccgatt
    10801 tgtctattat tcttcttttt acaaaagaac tttgttaccg gcctgacatc tggcggaaca
    10861 aaaggatagg agcagagtat catgcaaaca tttccgttaa actatttttc cgcattacgt
    10921 acgccaactc aagtgtttgc gggacgcaaa ctattatcat ggcctaaatt tttcttgata
    10981 tttgtatttt tagtttcctt gatggtcatg cctgtgactc ttttttatgc caatcagatc
    11041 caagcgattc cattagaaca atttctatcc gttcattcgc tgatcgatga acaaggaacc
    11101 caaaaatttt ctgaacttga attatctgaa actgggttac aagcatcgca acaaacgata
    11161 gctgtgaccc ctgaaatttt ggtaggtgtg tctttatctg agaaacaaca atccgatcat
    11221 ggaactttta ttgattttga gaaggaacaa tgggtgatcc aacaaaagga taaatctggt
    11281 atacgccgct atacgatgaa ttatagccca tctttccaac cagattctgt acgtacgccc
    11341 gaagatttcc aacgattttt agaaagagaa ttttatgcca gtaatcgtcc aacaatcatt
    11401 ttatcttata gtttaagtct tggtcttgtc ctatttgtca tgacgagtct cattttattc
    11461 ggggcttcct ttttcttgtg gatgacaaga aaaagccaac tttcatccat tcatacattc
    11521 aaagagagtg caaatttgat gctgaatgtt atgggaatag gttccatggc agcagcagtt
    11581 gtagggttta tccactttga ctttatcttg atgctaagtg tccaaacaac gataactgtc
    11641 ttgctgcttt tatgggtttt tgtcaaaaca agattcaaag ataagcgtgt cgaatgacaa
    11701 gtttaagcga tttatgcgac aatgaatgtg aaaaaaatga aaaataaagt agtttttgaa
    11761 aaatttctga aaaaattaaa cttcttagtt gattcttttc ttttttagta ttaacgtaat
    11821 agggtagttt ttttaaggag gaaaatcatg ttagagaaaa ctttatacaa tcaattattt
    11881 tcacgttctt tctcgctccc tgtcgaagta aattattggg acggtacaac gaaacaatac
    11941 ggagatacgg acactcctcc ccaaatcaaa atcaatatcc atgaagaaat cccaatgaaa
    12001 gagattacaa gtaacgcatc tttggcacta ggagaagcct atatggatca tcgaatcgag
    12061 atcgagggaa gtatccaagc attgatcaac gatgcttatc agcaagcaaa cagcttttta
    12121 cgaggaaaag attatctaaa atggctgcca aagaaagaaa aacatacgaa acaaaaaaac
    12181 aaagaagata tccatgcgca ttatgactta ggtaacgact tttataaact ttggctagat
    12241 ccaactttga cttattcttg tgcttatttc aatacacctg aagatacgtt ggaacaagca
    12301 caaatcaata aagtccatca tatcttagac aaactcttta tcaaagaagg cgaaacgttg
    12361 cttgatatcg gttgtggctg gggaacattg atgttcactg ctaccaaaga gtataatgtc
    12421 aaggcaactg ggatcacact aagtgaagaa caatacgatt tgattaccaa aagaataaaa
    12481 gaagaacatt tagaagataa atgccgtgta ttattaatgg attaccgcga actaaaaggc
    12541 gaaacgtttg atcatatcac aagtgtaggt atgttcgaac atgtaggttc tgaaaatctt
    12601 gaaggttatt tcaaagtggt aaaagattta ttgaaaccaa aaggaacagc tttgatccat
    12661 ggaatcagcc gacaacaagg cggtgcgaca aatgcttgga tcaataaata tattttccct
    12721 ggcggttatg tccctggtgt tactgaatta gttgataata ttacaaaaaa cgatttgcaa
    12781 ctgattgatt tggaaagctt acgtcgtgac taccaattga cattagaaca ttggacaaag
    12841 aattttcatc aagtccaaga tcaagtcacg aaagaaatgg gtgattcctt ctatcgaatg
    12901 tgggatctat atttacaagc ttgtgcagct tcatttgaat caagtaatat cgatgtgatt
    12961 caatatttgc ttgttcaacc tggaaacaat gacatcccaa tgcatcgtgc ataattgagg
    13021 caaaagtact gtttaaagac tatttaaaaa gaagctaagt tttatgatgc tccagaagtg
    13081 aacttttttg tctaacttct aggattgtct ctaaagctta gtttcttttt ctttatattt
    13141 tcataataaa agtttggttc tttaattttt aaacatga
//
LOCUS       DARDHA010000075        12961 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000075 DARDHA010000000
VERSION     DARDHA010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.076"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            209..1054
                     /locus_tag="SDA10_001677"
     CDS             209..1054
                     /locus_tag="SDA10_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG4240583.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            1245..2432
                     /locus_tag="SDA10_001678"
     CDS             1245..2432
                     /locus_tag="SDA10_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEG4240584.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            2438..2794
                     /locus_tag="SDA10_001679"
     CDS             2438..2794
                     /locus_tag="SDA10_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEG4240585.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            2796..3134
                     /locus_tag="SDA10_001680"
     CDS             2796..3134
                     /locus_tag="SDA10_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEG4240586.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    3210..3466
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3529..4563
                     /locus_tag="SDA10_001681"
     CDS             3529..4563
                     /locus_tag="SDA10_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4240587.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            4556..5242
                     /locus_tag="SDA10_001682"
     CDS             4556..5242
                     /locus_tag="SDA10_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4240588.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            5266..6114
                     /locus_tag="SDA10_001683"
     CDS             5266..6114
                     /locus_tag="SDA10_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEG4240589.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            6311..7084
                     /gene="sufC"
                     /locus_tag="SDA10_001684"
     CDS             6311..7084
                     /gene="sufC"
                     /locus_tag="SDA10_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEG4240590.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            7097..8383
                     /gene="sufD"
                     /locus_tag="SDA10_001685"
     CDS             7097..8383
                     /gene="sufD"
                     /locus_tag="SDA10_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEG4240591.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            8380..9615
                     /locus_tag="SDA10_001686"
     CDS             8380..9615
                     /locus_tag="SDA10_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG4240592.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            9602..10072
                     /locus_tag="SDA10_001687"
     CDS             9602..10072
                     /locus_tag="SDA10_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEG4240593.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            10077..11468
                     /gene="sufB"
                     /locus_tag="SDA10_001688"
     CDS             10077..11468
                     /gene="sufB"
                     /locus_tag="SDA10_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEG4240594.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(11563..12705)
                     /locus_tag="SDA10_001689"
     CDS             complement(11563..12705)
                     /locus_tag="SDA10_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG4240595.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12744..>12961)
                     /locus_tag="SDA10_001690"
     CDS             complement(12744..>12961)
                     /locus_tag="SDA10_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240596.1"
                     /translation="EFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITD
                     NINKLEQTISSIGKKYTKKKMKGGDTI"
BASE COUNT         4137 a         2433 c         2812 g         3579 t
ORIGIN      
        1 tagattttag taccaagatg aacgaataca ttgtaaaatg aataggaatg tatgattacg
       61 agcaagcaga cagctgattg tcttcatcta agttactagt tggatcagca actgttttgt
      121 tgtacatttc tattatagat ggttttttgt atatttaaat caaatagttt ttatagtaat
      181 tatcataaaa agttatggaa gtggtgccat gtttaagtta ttaacgactt ttcgagcagt
      241 atatgaaaca agaaattttt ccagagcagc agaagtactg tttttatctc aaccagcagt
      301 ctcgaatcaa atcaaacagt tggagcggga attgaatatc gatctttttc aacgtaacgg
      361 aagaaaagag atgcttccaa ctaagcaagc cgacttatta tatgaacgga cactggtttt
      421 gttagaagaa tgggaagaaa ctcagcaaaa attgctcaat gaacaaaatg aagaagaagt
      481 atgtcggatc gttgcttccc acacctttgc tgtctatctt ttgcctcagt tgatgaaaca
      541 tctgatcaaa aggtttccta aagtgcattt tgagatcgaa atcacgaatt ctcatgaatc
      601 attagaacaa gtagcgaaac atgaatccga ttttggtttt atcgaaaagc cattagagac
      661 ttccggcgta caaagatact ctttgatgga agaccagcta gtcatcgctg gggacccaaa
      721 aagcaaattg tggcttgtaa gagaagcaaa ctctggtgtt tttcattaca cgcagcgtta
      781 ttttgaagag atgaatatcc aagataagaa gatgatcgtg aaaagtaacg atgtaattgt
      841 tgcgttgctg aaggaaggaa tcggtcgctc gattttgtct aagcgctctg ttccagaaaa
      901 tgtgccaatg actccgttga atttccatcg atatttttat ttcatccaac gaaccaatct
      961 ggtttctgat cagctgctag agattgccga agagatcctt cgatacaatg aaagcacaga
     1021 aacttctcac agcgaggaga atctcagctg gtagataatt gcatgtatca aaaaaagttc
     1081 tatgatacac taaagataag cagtaggctt ttgtactgca tttgctcaag caactgcaca
     1141 aacggaaagt taatttttcc caaatgtcta tggaaagact ttccattatc taactggacc
     1201 gctataataa tcagcagtat ggtttttaag aaaagggatg aaacatggaa aaaaaattaa
     1261 aagcttcgaa cgataatcgt attgattatg gcgtgatttt accagtcttt ctgttatgtt
     1321 tgattggtct gctctcatta tatgtagcac ttagccatga tccgaacaat ccagcagtct
     1381 tgaaaggcgt agggatgcaa gttgtctggt atttagttgg agcggcagca atcgttgtca
     1441 tcatgcattt gaattccaaa tggatctgga aactgactcc ttacctttat ggtttggggt
     1501 tagtggtaat gggactcttg ctcaaattct atgatcagaa tttagctgct tcaacagggt
     1561 ctaaaaactg gtttcgattc ggtgcgttta ctttccagcc agcagagttg atgaagattg
     1621 cgtacatctt gatgatggca ctgatcgtaa ctaaacataa tacgcaagta aaagaacgga
     1681 cgatgaaatc ggacttttgg ctgattggga agctcttgat cgtgacaata ccggttttag
     1741 cattgatcat ggcccaagat gactttggaa cgatgcttgt ctttcttgcg atctttggcg
     1801 ggattttcct gatgtctgga atctcttggc gcatcatcgc acctgtagta atcttggcag
     1861 tagtcgttgg agcaggaacg atcttcttgg ttacgactga aggcggaaga gacttgcttt
     1921 ataaagttgg attcaaatct tatcagtttg cacgtattga ttcatggctg gaccctttcc
     1981 atgatacgtc aggaatgagt tatcagccgg cccaaggact tttggcaatt ggtaccggtg
     2041 gcttgtttgg taaaggattc aacgtaagca atatctatgt gcctgttcgt gagtccgata
     2101 tgatttttac cgttatcggt gaaaacttcg gctttatcgg tggtgcattc gtcattttct
     2161 tgtattttat tttaatctat cgtatgattc gggtgtgttt cgatacgaat aatgaatttt
     2221 acgcctatat cgcaagcggt ttgatcatga tgctgctgtt ccatgtcttt gagaatattg
     2281 gagcaaatat cggattactt cctttaacag ggatcccatt gcctttcatt agtcagggag
     2341 gatcctcgat tttagggaac atgatcggta tcgggttgat attatcgatg agataccaaa
     2401 atgaagcacc tgcaagatct tcaaggaggt aagaaaattg tacacctttt actggtaccc
     2461 gaaatgttca acatgcaaaa aagccaaagc gtggttagac cagcatggcg ttagttatca
     2521 aaccgttgat atgatcaaag aagtaccgaa ggcctcaacg attgagactt ggctagaaac
     2581 gagtcaagta ccaatccgtc gcctgttcaa cacaagcggg atgaaatatc gggaacttgg
     2641 actgaaagat caagtaggcg agtttacggc ttcaaaagcg agccaagtct tatcgacaga
     2701 tggtatgctg atcaagcgac cactgatctt acgagaggac cagcttgttg caattggatt
     2761 caatgaaaaa atttatgaag gagaactgat ataaaatggc aggcaagcat ttgaagaaaa
     2821 aggacaacct ttggatctta tttaatggaa aagaatactg tgttggtttg acaaatgaag
     2881 cgcaagaaga acttggagac atcacgtttg ccaatctgcc aaaagcagga caatcgctaa
     2941 aaaaagatga acctctgatc gaagttgaag cggaaaaagc ggtcaatgaa tttgccagcc
     3001 cgctaacggg tacagtttct tctgttaatg ataaaatcgg tgaagatatc aatatcttga
     3061 atgattcaga tgaaatgaat gcgtggatct taagtttcaa agatgttgat ccaaaagaat
     3121 ttgacgaatt ataaaatcag aaaatcactt gaaaaaagtt gacactgatt cttccagttg
     3181 ctataatcat aagcaatcct atatgaaacg agaaagcttt gaaaagaaga gtacatgtca
     3241 cggacgtttg aagagagccc tgtttgctga aaatgggtaa cgaaaatgag atggaagatg
     3301 gtcttagagc agagtaagcg agcgtaagca aacttactac gggagtgccc gttacagcgc
     3361 gacagtatac ggaaagaagc taagtccaag tactggaaga ggctgttatt gcgaagtaac
     3421 ggtaaatcaa ggtggtaaca cggaagttca agctttcgtc cttttgctta aggcaagagg
     3481 gcgaaggctt ttttgtttga aaaatcataa gagaaagatg tgaatggcat gtcattaatc
     3541 caactaacga atgtcaaaaa gacattcaat gggaaaaacg gaaacatcca agccgtcaat
     3601 gacgtttcac taaacgttga aaaaggggat atttatggaa tcgtcggcta ttctggcgca
     3661 ggaaaaagta cattagtccg tctcctgaat ggacttgagc ttcccacgag cggcgaagtg
     3721 atcgtaaacg atcaagatat tacaaaatta aaaaacaaag aattacgtac attccgaaag
     3781 aaaatcggta tgatcttcca gcatttcaac ctgttatggt caagaactgt acttgaaaat
     3841 atccagctgc cgctggaatt agctggcgta ccaaagagca aacgtaaaga acgggcagaa
     3901 gaactgttgc gtttagtcgg gttagaagga cgcggacaag catatccttc acaactttcc
     3961 ggcggacaga aacaacgcgt cggtatcgca cgagcattgg caaatgatcc tgagatcctg
     4021 ctttgtgatg aggctactag cgcattggac ccgcaaacaa cagaagaagt attagactta
     4081 ttgttagcga tcaataagaa attgaatttg accatcgtct tgatcacaca tgaaatgaat
     4141 gtgatccgca aaatctgcaa caaagtcgct gtgatggagt tggggaaagt cgtagaagaa
     4201 ggagacgtat tgactgtctt cagacaaccg aaaaaagaag taaccaaacg ctttgtacaa
     4261 caagacatcg aaccagaagc tgattcgaca gaactattag ctgaactcat caaagaaaat
     4321 ccaagcggcc gtttggcaac tttgaccttt agcgaaagaa atgccaacga gccagtcatt
     4381 tcccaagcga ttcgacgcta taacatcgat gtcaacgttg tgtatggaaa gatcaaacaa
     4441 gtgaaagaag gttcctttgg ttcattgact gtcttgatga ctggtgaaac caaagaatta
     4501 gatcaagcag aagtgttcat caaagaacaa ggagtaggaa ttgaggtgat ccatcgtgga
     4561 taaaagcttt tcagaaacat atctagattt taaacaaatc aacccggaaa cgatgaatca
     4621 ggctatcctt gatacactat atatgacatt aatctcaatg gtcttagtga cgatcatcgg
     4681 attagttttg ggattgatcc tttactctat tggacgcaaa aacaaaccgt atgctcgaat
     4741 catttatgga atcgtatcga tcatcagtaa tattttccgg tcaacgccat ttatgatcct
     4801 gatggttttg atcattccat ttactaaagc aatcgtaggc actatgctag gtgcgcaagc
     4861 agccattcca gctcttgttt tatccgcagc accattttat gcccgtctag tagagattgc
     4921 ttttcgtgaa gtcagccctg gtgtactgga agcagcagat gcaatgggag caagttactg
     4981 ggaaatcatc tggaaggtct tgatacctga aagtattccc gcattgattt ctggattaac
     5041 agttacaacg atctcgatga tcggatttac tgcgatggcc ggcgcgatcg gcgcaggtgg
     5101 acttggaggt cttgcttggc aggaaggcta ccagcgtggt aatctaacag tgacattcgt
     5161 tgcgacttta attattttac taatcgtatt tatcgttcaa ggaatcggtg atttcttaac
     5221 taagaaaaca gacaaaagat aataggatat agggggatgt aggatatgaa aaagaaaatt
     5281 tttggatttg cagcagcgct tttgctgact gtaggattag cagcatgcgg taacaacagc
     5341 gacagcaaag attcagcaaa taaagcagac gacacgacat tgaaagtcgg cgcttcaccg
     5401 acaccgcacg cagagatttt ggaacatgtg aaaccattat tgaaagatga aggaatcgat
     5461 cttgaaatcg tcaaattcga tgactatgta ttaccgaacc aatctttgga agaaggcgac
     5521 attgatgcca attacttcca gcatattcct tatttgaaca aagaaatcaa agaaaaagga
     5581 tatgactttg tcaatgccgg tgctgtccac atcgaaccaa tgggcttgta ctctaaaaaa
     5641 gtcaaagatg tttccgaatt aaaagatggt gcaacaatca ttacttcaaa ttctgaatca
     5701 gactggggac gcatcatcac aattctacaa gatgctggct tagtcaaagt caaagatgga
     5761 gtagatctgg aaacagcaac attcgatgat attgaagaaa accctaagaa cttgaagttc
     5821 aaccatacga tcgacccatc acttttagct tctacgtacc aaaatgatga aggtgaccta
     5881 gttgcaatca acgcaaactt cgcttacggc gcagggttga atccattaga agatgccgta
     5941 ttgcttgaaa aggacaactc tccttacgtg aatattattg cgactcgtaa aggtgatgaa
     6001 gacagtgaca aaatcaaaaa actgattgaa gtgcttcacc gcaaagaagt ccaagaatgg
     6061 atcacagaca aatggggcgg ctctgttaaa ccagtaaagg ctgatgcaaa ataaggtatg
     6121 gaacaaaaaa gatgagaaat aagtctgtaa tggcttattt ctcatctttt ttatacctat
     6181 tctaatttag aaatcaaaat tgtttaataa taaataaaag atatttgatt ttagtaagga
     6241 aggtcattgc gcttcattct caattagtct aaaatgagtg agaacgaaag agaatttggg
     6301 ggtataatgc atgtctgtct tagaaataaa aaatctacat gtgtcgatcg aggaaaaaga
     6361 aatccttaaa ggcgtaaacc taacaatgaa aaccggagaa atccatgcca tcatgggacc
     6421 taacggcaca ggaaaatcta ccttatctgc cgctatcatg ggcaatccaa actatgaagt
     6481 aacagaaggc gaaattttgt ttgacgggaa aaatgtttta gacttggaag tagatgaacg
     6541 tgcgcgactt ggcttatttt tagctatgca atatccaagt gagataccag gtatcacgaa
     6601 tgcggaattc atgcgtgcag cgatcaatgc gaaacgtgat gaaaacgaca aaatttctgt
     6661 tatggcattt ttgaaaaagt tagatgaaaa aatggcttta ctaaatatgc cagaagaaat
     6721 ggctgaacgt tatctgaacg aaggtttttc tggcggagaa aagaaacgga atgagatcct
     6781 gcaattgttg atgttagaac ctacatttgc aattttagac gaaatcgatt caggtctgga
     6841 tatcgatgct ttgaaagtcg ttgctaaagg agtaaatgaa atgcgcggag acaatttcgg
     6901 tgcattgatc atcacccact atcaacgatt attgaactat atcacacctg atgtcgttca
     6961 catcatgatg gaaggccgag tagtcatgac agggaatgct gacctagcta aacgtctgga
     7021 agcagaaggc tacgctggta tcagtaaaga attaggaatc gactataaag aagaagaagc
     7081 gtaaggggga gcaagaatga aagaaaaaaa ctggctcgat tatcttgacg cggtaaatga
     7141 tttttcttta agtaaaggcg aacctgattg gatgagaacc ttccgtcaag acgcactagc
     7201 gaaagcagat gaactgccat tgccgcatat cgatcgggtg aaattccatc gctggtcgtt
     7261 atttgatgtg aaagaaacac aaaccatcag tgaaacaggc acgattccgg catttgatgc
     7321 aatgaaagac aatcctgtgc ttgtccaaca aggttcatgg acgatttttg aacaactgcc
     7381 tgtggaatta gcagaaaaag gagtcatttt taccgacttg ttcactgcaa tgatcgaata
     7441 tccagaacta gtccaagaat attatatgaa aaaagcagta aacatgaacg aagaccaatt
     7501 gactgcactc catgtagctt ttatgaacag cggtattttc ctgtatgtgc ctaaaaatgt
     7561 cgtgatcgat gaaccactcg agtccttatt cattcaagat ggcgcatccg atgagcactt
     7621 cttcaagcat gtgttgatcg tggcagatga acatagtgaa ttttcttatc tggaacgttt
     7681 ccagacaacc aaagaacaag tagcaaaatc atcaggtaat atcatcgtgg aagtcatcgc
     7741 taaagctggt tcaaaaatca aatattcagc cgtagatcaa ttaggagaaa atattacttc
     7801 ttatatgaat cgccgtgggc acattttacg agatgcttct gtcgactggg caattggtgt
     7861 catgaatgac ggtcatgtga tcgctgactt tgactcagat ttagcaggag aaggtgcaca
     7921 cgcagaagtc aaaatcgtgg caatcagctc gggcagacaa atccaaggaa tcgatacacg
     7981 agtaaccaat aaagcccctc atacgatcgg gcatattttg caacacggag tgatccgaga
     8041 aaaaggaaca ttgaccttta atggtatcgg ccacatcttg aaaggggcaa aaggtgcaga
     8101 tgcccaacaa gaaagtcgtg tcttgatgct ttctgataaa gcacgtggtg atgcgaatcc
     8161 tattcttttg atcgatgaaa acgaagtaac tgctggacat gctgccagtg taggacgagt
     8221 tgatccagaa gaaatgtact atctgatgag tagaggattg cataaagaag aagcagagcg
     8281 cttagtgatt cgaggattcc tcggttctgt cttaacagca atccctgtag aacaagttcg
     8341 gaaagaatta gtcgaagtaa tcgaagggaa gttgaatgga tgatcgatgc taaaaagcta
     8401 cgcaatgatt ttcctatctt atttcaagac gtcaatgatg aacctttagt ttatctggat
     8461 aatgcagcga cgacccaaaa gccaaaacaa gtactccagg cattagcgta ctattacgaa
     8521 cacgataacg ccaatgttca tcgcggtgtc catacgttag cagaacgcgc gacccatgat
     8581 tatgaacaag caagagaaaa agtacgggcg ttcatccatg caaaagaaac agcagaagtg
     8641 ctgtttactc gtggtacgac gacaagcttg aattgggtag ccagaacctt cggcgaccgc
     8701 tatatcaaag caggagacga gatagtgctc tcttacatgg aacatcattc taatgttatc
     8761 ccgtggcagc agttggcaaa acgaacaggt gctgtcttga aatatatcga actaacagaa
     8821 gaaggcttcc tggatatgga ggatgcagcg aaaaagatta cggacaaaac caaaatcgtc
     8881 tccattgctc atgtatccaa tgtcttaggt gtcttcaatc ctgttcaaga attagctgaa
     8941 cttgtccatg aaaacggcgg ggtcatcgta gtagatggtg ctcaatctgc cccgcacatg
     9001 gccattgatg tccaagcact agactgtgat ttctttgctt tcagcggaca caaaatgtgc
     9061 ggaccaacag gtattggtgt tttatatggt aaacgaaaat ggttagaaga aatggaacct
     9121 gttgaatttg gtggcgaaat gatcgatttt gtcgaattat acgacagtac atggaaagaa
     9181 ctaccttgga aattcgaagc agggacacca aatatcgccg gcgctatcgc attaggccat
     9241 gcaatcgatt atttgcaaaa actcggtatg gacaatatcc atcgatatga agaagaacta
     9301 gccgcttatg tgttgccaaa attacaagca attgatggat tgactgttta tggtccacaa
     9361 gacccgaatt ttcggacagg agtcgttgca ttcaatttgg atgggttaca tccccatgat
     9421 gtcgcgacag ctttagacat ggaaggggta gccgtcagag caggtcatca ttgtgcgcaa
     9481 ccgctattga agtaccttga agtatcagcg actgctcgcg cgagctttta tttctacaac
     9541 acaacgcaag acgccgaccg tttagttgaa gcaatcatgg caacaaagga gtttttccaa
     9601 catgggacta tctaaattag acaatttata ccgtcaagtg atcttggatc attcttccca
     9661 tccgcatcat cgtggaagct tgactgactc gacaagcaag atcgaattga acaaccccac
     9721 atgtggagat gtgatcgaat tgcaagtcga tgtaaaagac ggaatcatca aaaatatcgc
     9781 ctttgacggc agtggctgct ccatcagtac ggcaagtgcc tcgatgatga cagacgccgt
     9841 catcggaaaa tcaatagaag aagcagaaca tttaactgta gactttctac agcttgtcca
     9901 aggaaaagaa gtcaaagatc ccgaccaatt aggcgatgct gctatgcttg gtggtgtagc
     9961 taaatttccc gctcggatca aatgtgcgac acttgcctgg aaagcactag gaaaagcaat
    10021 ggaaaacgat ggtaccgcag ctttccatca ttaccacaag gaagaggagt gagaacatgg
    10081 gcgtaccaga attagaagaa tataaattcg ggtttcacga tgatgtcgaa ccggttttca
    10141 gtacaggaga aggcttgaca gaagaagtga tccgtgagat gtcaagaatc aaaggcgaac
    10201 cagaatggat gcttgaattc cgtctgaaat ctctagaaac atttaacaaa atgccaatgc
    10261 aaacatgggg ccccgacctt tcagacatcg atttcgatgc catcaaatat taccaaaaac
    10321 caagtgacaa acccgctcgt gactgggaag acgtaccaga aaaaatcaaa gatacatttg
    10381 aaaagattgg tataccagaa gctgaacgag cttatctagc cggtgcttcc gcacaatatg
    10441 aatcagaagt cgtgtaccat aatatgaaag atgaattcga aaagttagga atcgtcttta
    10501 ctgacactga ctctgctttg aaagaatacc ctgaattatt caaagagtac ttttcaaaac
    10561 ttgtaccgcc aaccgacaac aaattagcag cgctgaattc agccgtatgg tcaggtggga
    10621 cctttattta cgtgccaaaa ggcgtcaaag tcgatgttcc attacaaacg tacttccgga
    10681 tcaatgcgga aaacacgggg caattcgaac gtacgctgat cattgttgac gaaggagcaa
    10741 gcatccacta tgtcgaaggt tgtacggcac ctacttattc aagtaacagc ctgcatgccg
    10801 ccatcgtaga aatctttaca agaaaagatg cgtattgccg ttacacaacg atccaaaact
    10861 ggtcagacaa tgtgtacaac ttagtaacca aacgagcaaa agcatacgaa ggcgcaaccg
    10921 ttgaatggat cgacggaaac ctaggtgcaa aaacaacgat gaaataccca agtgtttact
    10981 tagatggaaa aggtgcacga ggcaccatgc tctccatcgc ctttgcagga gcaaaccaaa
    11041 tccaagatac gggtgcaaaa atgatccaca atgcaccaaa tacatctagc tctatcgtct
    11101 ctaaatcaat ttcaaaagac ggcggagaag taaactatcg aggacaagta acctttgcca
    11161 aaaacagtgc gggttcgatc tctcatatcg aatgtgacac gatcatcatg gacgaatggt
    11221 cgaaatccga tacgatccca ttcaatgaga tccacaacag ccaagtctct ttagaacacg
    11281 aagccaaagt ctcaaaaatc tcagaagaac aactctacta tctgatgagc cgaggcctga
    11341 ccgaatctga agcaactgaa atgatcgtca tgggctttgt tgaaccattc accaaagaac
    11401 ttccaatgga atatgcagtc gaactcaatc gattgatcag ttatgagatg gaaggcagtg
    11461 ttgggtaaac ctgttatagg agctagaacc cttgatatat aagggttcta gcttttttgt
    11521 tttgcctgta tactagaaat gaccagattt tgaccagctc ttttactgat gagccatcat
    11581 atcagcaaag tcttgacctg ttttcttagg tgcattattt gtaatatgtg catagatgtc
    11641 taatgtcact ttactatttt catgccctaa tcgttgttga actaccttga cagattctcc
    11701 tgcttctaat aataaactag catgtgtatg tctaaaccca tgaggtgtaa tacgaggcaa
    11761 gttatatttt ttcaaaatat atcttaacca atcattaaca gtgtttggaa tgtatagagt
    11821 gtttttaact gttgtaaaaa catgttgctc tttagagcta gtattgtatc caagttttaa
    11881 atactcttct ttttgctgta atttccagtt gttgaggata gaaagagtct cagtatctaa
    11941 agagataact cgtctagaag ctcttgtttt cggagattga attataactt taccaaattc
    12001 atctacagca agagttttat tgacatgtag ttctttatta aacgtgtcta tgtctttcca
    12061 ctgtaaagcg agtacttcac tctttctcat acctgtgtat gccaataatc tgaagaaggc
    12121 gtattgtttc atgttaccaa aatctttaaa agcatccagt aaattattaa gctcctcttt
    12181 tgagtaaaat ttacgatttt tctcatactc tttttttcgt ggaagaagtg ttttttccat
    12241 aggattattg tcaatatatt gcattgcaac cccgtagcgt aaaatttgtg cagtctgttt
    12301 tctcatataa tcgtaacttt catatttact gtgccattca tctaccactt tttgacaata
    12361 tgggacagag atattcgata gtttaagctt accaaatgct ggaagtattt gattatttgc
    12421 gtatcttact gagattgcga ttgttgaagc tttaacactg gtacgctgat gagttatcca
    12481 ttcattatac aggtcttcaa atgtcatatc aggtgctttc tttttaacac cgtataatat
    12541 ttgatgtctg tactcgtcat aatctttttg agcttcttgt ttgctactaa aacctcttct
    12601 cgtagtattt attttttcac cagttcgtgg atcggtggca atgtgtcctt tatacatcca
    12661 cgccacttga ccgtttttct ttttgtactt tgatattttt gccattaaat catctcctct
    12721 tagtttacaa ggagtactta ccactagatg gtatcacctc ccttcatttt tttcttagtg
    12781 tattttttcc caattgatga aatcgtttgt tctagcttgt ttatattatc agtaatttct
    12841 ctcacagctt cttcgggata agacatgtct ttcaattgaa taggtagcca ttcatctacc
    12901 attctttcta gtagttcttc tatactgcta gtgaggaaaa gaatgttatt gtcaaattct
    12961 t
//
LOCUS       DARDHA010000076        12734 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000076 DARDHA010000000
VERSION     DARDHA010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.077"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="SDA10_001691"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="SDA10_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEG4240597.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="SDA10_001692"
     CDS             complement(322..891)
                     /locus_tag="SDA10_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEG4240598.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="SDA10_001693"
     CDS             1077..1979
                     /locus_tag="SDA10_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG4240599.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="SDA10_001694"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="SDA10_001694"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEG4240600.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="SDA10_001695"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="SDA10_001695"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEG4240601.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="SDA10_001696"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="SDA10_001696"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEG4240602.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="SDA10_001697"
     CDS             8547..8732
                     /locus_tag="SDA10_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HEG4240603.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="SDA10_001698"
     CDS             complement(8794..9990)
                     /locus_tag="SDA10_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG4240604.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="SDA10_001699"
     CDS             complement(10160..11596)
                     /locus_tag="SDA10_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEG4240605.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="SDA10_001700"
     CDS             11889..12698
                     /locus_tag="SDA10_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG4240606.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DARDHA010000077        12679 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000077 DARDHA010000000
VERSION     DARDHA010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.078"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(330..692)
                     /locus_tag="SDA10_001701"
     CDS             complement(330..692)
                     /locus_tag="SDA10_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240607.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(766..1146)
                     /locus_tag="SDA10_001702"
     CDS             complement(766..1146)
                     /locus_tag="SDA10_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEG4240608.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1210..2403
                     /locus_tag="SDA10_001703"
     CDS             1210..2403
                     /locus_tag="SDA10_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG4240609.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2480..3064)
                     /locus_tag="SDA10_001704"
     CDS             complement(2480..3064)
                     /locus_tag="SDA10_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG4240610.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3265..4263
                     /locus_tag="SDA10_001705"
     CDS             3265..4263
                     /locus_tag="SDA10_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG4240611.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4375..4884
                     /locus_tag="SDA10_001706"
     CDS             4375..4884
                     /locus_tag="SDA10_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEG4240612.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4914..5573)
                     /locus_tag="SDA10_001707"
     CDS             complement(4914..5573)
                     /locus_tag="SDA10_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEG4240613.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5575..6339)
                     /locus_tag="SDA10_001708"
     CDS             complement(5575..6339)
                     /locus_tag="SDA10_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEG4240614.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6347..7024)
                     /locus_tag="SDA10_001709"
     CDS             complement(6347..7024)
                     /locus_tag="SDA10_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEG4240615.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7061..8446)
                     /locus_tag="SDA10_001710"
     CDS             complement(7061..8446)
                     /locus_tag="SDA10_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG4240616.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8415..8669)
                     /locus_tag="SDA10_001711"
     CDS             complement(8415..8669)
                     /locus_tag="SDA10_001711"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240617.1"
                     /translation="MSDYNETTTDFRVCFFYFYSTVKMNVPDFLARLIYRILYLYNLL
                     QGRKNSEIELVSMEEKAAYGMIKIKRGERVHGKNSDFTYE"
     gene            complement(8656..8740)
                     /gene="ffs"
                     /locus_tag="SDA10_001712"
     ncRNA           complement(8656..8740)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="SDA10_001712"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8763..9278)
                     /gene="tadA"
                     /locus_tag="SDA10_001713"
     CDS             complement(8763..9278)
                     /gene="tadA"
                     /locus_tag="SDA10_001713"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEG4240618.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9385..10242
                     /locus_tag="SDA10_001714"
     CDS             9385..10242
                     /locus_tag="SDA10_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4240619.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10323..10658)
                     /locus_tag="SDA10_001715"
     CDS             complement(10323..10658)
                     /locus_tag="SDA10_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240620.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10725..12095)
                     /locus_tag="SDA10_001716"
     CDS             complement(10725..12095)
                     /locus_tag="SDA10_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEG4240621.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12173..>12679)
                     /locus_tag="SDA10_001717"
     CDS             complement(12173..>12679)
                     /locus_tag="SDA10_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4240622.1"
                     /translation="EYLLFKSMVYVEEYGMKSITARELAEFCGCSTYPIYTHFKSISG
                     LKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAKSDLDMDSLFK
                     RPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWDKNEVTDEYLE
                     NILKEQSP"
BASE COUNT         3772 a         2567 c         2122 g         4218 t
ORIGIN      
        1 actcagttat cttatcatgt cgttagtaac atgtcaataa ctatttttta aattttttag
       61 aaatgtgata taacatccac ttcagaatca acgctgttaa atttatcatg tatttcttcc
      121 ttggtcaact ataaattaag cattactttg tgaaatgatt tttcgatcaa ttattattat
      181 tcgttataca accgttttca acatattttg aaaataaaat gcactaaaaa actatcttta
      241 gcagaacatt tcattgtatt ttttggacca aacaataaaa aaagaagttt gaaaaaaatt
      301 caaacttctt tgttcgctta atctgtattt tagaagaaag gcactccctc tacttcgtaa
      361 gttccaagaa cagagcgcat ttgacctggt tcttcccaaa tatatccttc gcctaattct
      421 tctctttgat tcttttgttc agtttcaaag acaaacaacg gaacattttc ctctttaata
      481 tgtgtatttg tcaattccat caaactaatc ttgctaacat cgatatgaac tatttctttt
      541 aagaaattaa gtatattcgc taaaccagtc tttccttcag aaagttccgc taacgcaaat
      601 tccaacttgt catcaattga atggaccaaa aattttttag taccatcctc caaatgcaac
      661 ataattgtac cagcaactct tttgcttgcc aaaatttcca cctcgatcaa cttgttctat
      721 ggcttacata aaaattctag cacattttca gaaaaaatac actttttatt tcttattttc
      781 tttccgttct agttcagata atgcttcttc tatccaaata ggtaactgct ccgcaatagt
      841 ggtaaatccg atcttacgat ttttattcgt gaaatacctt tttctgcgat atggaacagc
      901 tggatttgtt ttttctaacg ttcttaaaga taaactgatt tttttggaat attcatcaat
      961 gtcgatgatc tgaactgtta ctggctgtcc gacttttaac aatgaatgga tatttttcgt
     1021 ataaccggat tgtacttctg aaacatgaat aagtccttgc gtctcgttat caagagaaac
     1081 gaatgcgcca taaggctgta tccctgtcac ttttccttca attatttgtc caattttata
     1141 agtcatctac ttccctctct tcgcatttat cattatactg taaatagctc aaaaaaagaa
     1201 gcgacgaaaa tgactggagg tcttaagatg atcgaatttg ataaacagat ttcaagaaaa
     1261 gatacagaaa gtgtaaaatg ggacgcaatt gctgagacat atcaaatgga tgacttactt
     1321 cctttgtggg tagcagatat ggacttcttg tctcccggtg gggtagctaa agcgtttagt
     1381 gaatacatca aacatggaat ctttggttat gccactctgt ctgataaact atatcaggct
     1441 atcattcatt gggaacgcca acaccatgct gtggagttga cgaaagaaaa tatcgttttt
     1501 acaagtggtg tgttaaccag tttagctgca gcagtacaaa cttttacaaa accaggcgat
     1561 tctattttga ttcatgaccc agtttatcct ccattttcct ctattataga aacaaatcaa
     1621 cgtcacatcg ttcgaagctc tttgctagaa aaaaataatc atttcatcat ggacttgtct
     1681 gacatggaga aaaaaatcga agaaaacaat gtaaaagcaa tgattttatg caatcctcac
     1741 aatcctggag ggcgtgtttg gagcaaagaa gaattgaaac agttaagcga attgtgttta
     1801 aaacatcaag tgattctgtt tagtgacgag atccatcaag atttaacttt attcgatcat
     1861 acatttactt ccatgctaac gattgatcct aatttagatg atctactagt tgcttttact
     1921 tccgcaacca agactttcaa tctagctgca atcaaaaatt caatggtatt tatcaaaaat
     1981 ccagagatga aagctgattt tgaaaaacat ttagtgatga accaacagca cgaaatcaac
     2041 acttttggac tgattggaac ccaagcggct tatgagacgg gtggagaatg gttaagccaa
     2101 ctgattcctt atctagaaaa aaatgttcaa acagttaatc agttttttga acagtatctt
     2161 ccgaaagtga gaataatgaa ccctgaagga acttacttaa tgtggttaga ttttaccgcc
     2221 tatatttcag acgaccaaga attggagaac actcttgttc ggaaaggaaa agtcgtatta
     2281 aatcctggca tcacgtttgg tccttccggt catgggcata tgcgcttgaa tattgcttgt
     2341 ccagaagaaa ctttactgga aggacttaat cgaatcaagt atgctttaga gacaatcgat
     2401 taaatcattt attcaaaata aaaggacgag accttactag gtttcgtcct ttttcataat
     2461 tactccgtac cataccaaac tattctgaaa tttcaattgt ttcaatcact acatcatgta
     2521 caggtcggtc ttgtggacca cgttgtacat ttgcgatttc atctactgtg tccatccctt
     2581 ctagtacatg tccaaagact gtatgtcgga aatctagcca aggagttccg ccacctttat
     2641 acgcttcaat gatttctgat gggaatccag caccttctag ttgtccaagc atattagctg
     2701 gcacatttgt gttatttaca ataaagaatt ggcttccgtt tgtgtttgga cctgaatttg
     2761 ccatagataa agctccgcga aggttgaaca gttcacgact gaattcatct tcaaaacttt
     2821 cgccataaat actttcgcca cccattcctg ttccagttgg atcgccgcct tggatcatga
     2881 aatcaggaat aacgcggtga aaaatcacgc catcataata accttttttg gaaagttcaa
     2941 tgaaattttg aaccgtcttc ggtgctagtt cagggaataa ttgaacagta atgtcaccac
     3001 ggtttgtttt gataacagct tttggtcctt tttcactttt taaatctagt tgtgggaatg
     3061 ccatattgtt tcctcctaat atttgcatac ttcataataa taataacata atacctaacc
     3121 tctcgtaaaa atactagatt tgttttcttt tatttttcat cactagatac attctataac
     3181 ttaccgtttc gatttcgaat tcaaacattt ttgagaagca ttctcaatta aattctgcta
     3241 tagtgtgaaa agggaggaat ttttatggaa atctatgaca ttacaatcat cggtgcaggt
     3301 cctgtaggga tgtttgccgc tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt
     3361 gacagtctac ctcaacttgg tggtcaatta gcaactttgt atcccgaaaa atatatttac
     3421 gatattcctg ggtatccagc aataaaagca agtgaattaa tcgatcaatt agaaaaacaa
     3481 ttaactactt ttaaccatac attccattta aaagaagaag ttctctcgct tactcgtgaa
     3541 gacgaagtta tcgagattac tacaaataaa ggcatccatt attccaaagc agtcatttta
     3601 gctttaggaa atggttcatt ccagccaaga aaattgaatt tagataatgc tgaatcattt
     3661 gaaaaccacg gattggatta tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc
     3721 gctattgctg gaggtggtga ttctgctatc gattggacgt tgatgttaga gcctattgca
     3781 agcgaagttt acttgattca tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca
     3841 cgcttgaaat cttcatctgt caacttgctt actccctatc tcatcgatgg attatcaggc
     3901 aataatgggg aactgacaga tatccggtta aaaaaagtga aaagtgacga gacgattgat
     3961 ttgatgattg attctctgat cgtcaattat ggattctctt ctaatttaga acatctttct
     4021 tcttggggac ttgatagtac cagaaatgcc attactgtaa aatcagatat gtctacttct
     4081 attccaggcg tttatgcagc tggtgacatc tgtacctatg aaggaaaagt gaaactgatt
     4141 gctactgggc tcggtgaagc acctactgca gtgaacaacg ctttacacta tattaatcca
     4201 aaggaacgaa ctcagcctgg tcacagcaca agcttatacg ataaaaatct tcgtcctagt
     4261 tgatttttta tatctctttg catttcaagt taaacccgaa agcagtttct tgcatttttt
     4321 tcatgttccg ttaacatgaa atagagaaag aaaaaataaa gggggagcaa taatatggtc
     4381 gtcaaaactg agactttaga agaaaaggca agagaactat tgaaggaacg tggcgtaact
     4441 gtagaagata tcgcacaact tgtcatgttt ttacaaaaag actatatttc tgatttgact
     4501 ttggcggatt gtactgaaag tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc
     4561 attatcactg gaatccaatt agatatatta gcagaaaaga aaaaattgat gagtccgttg
     4621 caacatatta tcgaagatga cgaaggcctt tacggtatcg atgagatcct agccttgtca
     4681 atcgtgaatg tttacggtac gatcggcttt acgaattatg gttatatcga caaggtgaaa
     4741 cctggtattt taaaagaact aaacagccac gatggcgttc atgtccatac cttcttagat
     4801 gatatcgtcg gagcgatcgc cgctgctgct gccagtcgat tagcacacaa agaaccagaa
     4861 aaaagaagtc gccttactca ataattcaac aaaaaaagaa agaaaacgtc tatttagacg
     4921 agttctttct tttttgtttg tttcaagcta ttttttccta agaaaagaaa aacagcaaaa
     4981 aataattcta ataatacaaa aaataagata aagctatgca tgaaaaattc ttctggaagt
     5041 acattgatga ttggatcagt aacgggatcg aataaccagt catcgttgtt gaaaaacgtt
     5101 tcatgaaaaa ggataaaaaa gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact
     5161 ggcagtaaca tgccgatttg aaacggacga atcaaccgcc aaagccgatc attcttttgc
     5221 aaatatttga taaacaaaat actcgggatc aaagtgatga aaaacacagc ataatccact
     5281 aaaaacaaaa ttttcacttc ataaaagtga tgggctccgc tagcagaaac tgggaaatct
     5341 ggcaagctga ggatggtttt gaagggatta ttgagataat tcattaggtg atcaaaattt
     5401 ttcagtaacg tctcttgatc taaagaagta taatccaaaa tttgcaaatg tccgatatca
     5461 aatacgtata gtggtcgaaa gttgatcgtc acaccaactg ccagactgat cagtgtcaga
     5521 aataggctga acaatccagc atattccatc cactgccatc tcgaattttt catattagaa
     5581 gctccattca tccagtgatt gtttcacata ggtagctggg ataggcaacc caggtacatc
     5641 ttcttctttt gtaaaacctg ataaaacgag caaagtatct atattattgc gtatcccagc
     5701 ttggatatct gtttcatagt tatctccaac catgattact tcttcttttt gcagccctaa
     5761 tacttcaacg gctttatcca taatgatggc ttctggtttt ccgatgtaga tcggcggcgt
     5821 ttgggttgct gtctctacaa aagagatgac tgaccctgct ccaggaagaa ggccgcgttc
     5881 agtaggtata ttcttatctg ggttcgttcc aatgaaagta gcaccttttt gaatagcaag
     5941 tgttgcttta actacttttt cataggtcag ataattatct aagcctacca ctacatagtc
     6001 aggagcctct tcttcccatg taaatccggc agctaaaatc aagtcaatca atcctgcttc
     6061 tccgataaca tagacttttt tcccttttcc atctgctttc ataaaatcga ttgttgccaa
     6121 agtagctgtg tagactgtct ctggagctac atgaatgtca aactcatctg ctagacgatt
     6181 agctactgtt tctggtgact tagtcgtgtt attggtaaca aacaagaaag gtaattttct
     6241 tttttgcaat tcttccacaa atcgtttacc ggccggtatg ggttgtgtcc cacgataaat
     6301 cgttccgtct aaatcaatta aatatccttt atagtccatt catccactac tcttcccttt
     6361 ttctgatcgt aaatgctgtt tttgtttttt ctacttgttt aggttgttct tgttttttta
     6421 ctttttcaga ggtttttgct gactctgttt ttcgattttt aataaccggt tttttgcgat
     6481 tagatgcgac tggtcctttt ttctcatcga tatacgcacg attatggttt tttttctttt
     6541 ttcggcgctg agttgtcttt tcttttttac cgccgattct ctcgatgaca aaatacgcac
     6601 aaccaaaatt gcaatattcg taaagatagt cttccaagga atcgattcgc tgatctggat
     6661 gcgcccgtcg attatccgct tggaaaaatc cttttaggcg tagttgttca taaccccaat
     6721 ctcctacgat ataatcatat cgggctagta catcactgta acgttctcca agcttttctg
     6781 cattaaatcc ttcacgataa tcggacacaa gccgataatg tctttcacct atcaaaaaat
     6841 tactgtcatc taacagtgtc actagctcgc ctttgacttt ttctgcttta ggctcctcaa
     6901 cgatttcttc caaagcagct gtcagttctt cggtgactgt tgtttcctgt tcagctgatg
     6961 attttttctt agcaatttct gttttatgct caacgttctg ctgttcattt tgttttgcag
     7021 acatgcgttc accacctatc ttttttatta taccttgttt ttatttgatt gggtagtgag
     7081 ctttcaagta atctcctacc acacttcgga taaattcagg gaacaaaaat tcattttttc
     7141 cagcaatctc aatcgttgga aaaaatggaa caaacatcaa atgatctacg gtagtaaaag
     7201 tataattttt attcagatcg attggcttgt tcttccaaaa gacttgatgg ttaatagaat
     7261 catatgtgat tccattatag accatctgcc caaaaatctt accgcgaaat cccattccct
     7321 tcataggata gttacgtaaa aagttacgat ttttttctat ttctaaaact aagcggatca
     7381 gatcatttcc acccaatgtg acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa
     7441 gctgatcctg attgacccgc ccttttggaa tttcagttaa aaacagacca ctgtttaata
     7501 ttgctgcttc cgtctttcct cgttctttca ccgcctcaaa ggctacttgg ataaacgaat
     7561 gctcattgaa gatatctaga ctcagagaaa atggcaaatc tgctacttct ttttctttta
     7621 ataattcatg cccacgttct agataactat ttatttcttg ttcatcttct gtaaaagctg
     7681 tcattgtttc ggtagggatg gctttggctg aatgcttaag gatttttttc tcttcatcga
     7741 tggttaaatg gacttcaccg acatactgcc cgtactttcc agcggctgct atttggacat
     7801 cattgattac gtgaccttct ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa
     7861 ttgctggtat ttcttttgca atttggaaat catcctgaat cccgagatga ctcatcaaaa
     7921 ctaaaacatc cacttgtgaa cgtaaatctt cgactaattc tggcaaaata tcataaggat
     7981 tccgtatgtc ccagccattc ggattataag taagcggaaa tggagcagta aatgcaatta
     8041 gtccgatttt cgtctggtgt ttcgttgtaa tgattttata tggtttcgtc catgccggtg
     8101 gctggagcgt atttttatcg aataagttat ccaggatgat atcaaactca gcatgatcgt
     8161 ataaatgatt caactcttct ttggaatttc ctacgccttc attgtttcct atcgtcgctg
     8221 catcataacc aacttcattc atcagcaaga tattttcctg tccattcgtt gcttctgaca
     8281 atggatgcca acgatccaca aaatcaccta gatctactgc aatcgttgtc gttttttctt
     8341 tcttttcgtt ttctcgttta cgttgatcca aaaaccttct gatccttggc caattttcta
     8401 aatgagaatg cagatcattc gtatgtaaaa tcactatttt ttccatgtac cctctcgcct
     8461 ctctttatct ttatcatacc ataggcagct ttttcttcca ttgaaacaag ctctatttca
     8521 gagtttttcc tgccttgaag caaattgtat aagtacaata ttcgataaat cagtctggct
     8581 aaaaaatcag gaacattcat ttttacagta ctataaaaat aaaaaaaaca gacccgaaag
     8641 tctgttgtcg tttcattata atcagacaca tggcggaacc tgcttagtgc tgcttccttc
     8701 cagacctgac acgcttcaca agcccaccat tgtcctagcc tttcggcaaa gaatagtata
     8761 ccttattttt cattcgttga ccagtctttt tttaatttct tttttcgctg gcggagcttt
     8821 cggaaaaagt cagtcaaaat catcccacat tcgtcttcta aaatgccacc ttctacatat
     8881 gcttggtggt taaaccgctc gtcctccaaa agattcatca aggtaccggc tgttccgcct
     8941 tttggatcat acgcgccgaa ataaacttct gctacacgag acaaaatcat cgcgccgctg
     9001 cacataggac aaggctctaa ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc
     9061 actttttcac aggcttcctt gattgcaagc atttctgcat gtgtagtagc atcttgcgtg
     9121 gtttctctaa ggttatatcc tcttccaatc acttttccat ccaatacaac cactgctcca
     9181 atcggaactt catgaagcat ttctgctttc tttgcttctg ccaatgcaag gcgcatccat
     9241 ttttcttttt ctactgcatc aagatttgta tttaccaatt acccttccct gcctttgttt
     9301 tttcttgttt tactttagca tatgataaaa aggctgtaac taactttttt ttctatggta
     9361 aaataaatac aagaggtgaa caagatgaaa atggataaat tacttgctct ttacccacaa
     9421 gcacagaaaa aaaactctcc agcatcagaa gcggatatcc tttcttttgc aattgatgat
     9481 gcttttatct ggatcaaaca agattcatta tctcaacaag aaaccagcct tttgaaagcg
     9541 ttattccctg taatcaacga tcaaaaaaaa cacccatggt atcgctatct ttttaaagat
     9601 gcttcttgtg ttcaagaaaa aagctttcgt ttgatccagc ttcacgtcga atcaaaagga
     9661 gaattcttaa aaaaagaatg gcaagatact attttggaat tatttcgcta ttctgctgat
     9721 ttcttttttt atacagacac agatgcgctc ttagtcgaac aaataaaccg tgattatctt
     9781 gatgctactg aaatcaacgg tatttttcta tctttagatg ctgattttga tacgacaacg
     9841 tctgtattcg ttggttcttt ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa
     9901 gaaagaaaaa tttttttaac agagcaaaac aatctttata ctcacagccg tactttttct
     9961 atgcaagatg tggctttaca ttactataca aaagatactg taaaagatag taagttgatc
    10021 aaaagttaca tagaactgat caaacaaaat gaaatggaag tgatcattca agaattatgg
    10081 aatgaattag gcaatgtcag ctctgctgca aaaacactgt ttcttcatcg taatactcta
    10141 aaatatcgaa tcgaaaaatt tcaagaacag tctggtttca atctaaaaaa agccaacgat
    10201 ttactgtttt gctatctttt attgcttcat gaacataatt aaagatagat taaaggggtt
    10261 gtgacaataa ttttgtcaca accccttatt tcgaatgaat agtgttcaaa agctagtttc
    10321 tcttacatca ttttttcaac aaaatgcatt agccggtctt ttacatcttc ggttgaatct
    10381 ttgattgaat caccataaac agagattttc ttttttcctg atttgatttt tgctaacatt
    10441 cccatcaaag aatccaagtc actatctgaa agaccatcta cgatttctag taatttttca
    10501 tttccatcaa atttgtcata gacgaatttt tttactttcg tccgagtagt catatgatgg
    10561 atcttttcca ttacttttcc cgatacaacg actgctgtcg ctacactggc agcggctgct
    10621 acgcttaatc cgattgttac ttttgttgat gttttcataa gtatcagctc ctttttatct
    10681 atctccatca tagcatagac aaaaaaataa acgaactaat ccactcagtg aaatctcaat
    10741 cctttaggga aaaagttctt tactgttcct tttacttgtt tcccaaatcc gacaggtatc
    10801 ttatctaata ctagcaaaac ccatccttga ttcccatcac gttgaaatgt ttctcctgct
    10861 gtgtaagatt gccattcctt ttgagtgatc ggcaagcaag gtatattctc tatctttttt
    10921 gtagcaagcg ctaatgcata gctaggttca aatcgattct ttttgaaatc tcctaaatgc
    10981 aaacctgtac gtacgacttt taaaccatcg agagaagggg ctaactcagg aacttcccaa
    11041 agatggtcgt tgaacactaa aagtcttcca gtagcttcat aatgaaaatc atttgaaaac
    11101 tccgtccaga gtttttcctg ctcttttgtc atttgcactt tacttttttt tcgtgtcttc
    11161 ttttctttat gcatctgatt ttgtccatga aaagtcagct ttgcaacaaa atgtccttca
    11221 ccctgatctt tatgaggcca tatacgtatt gttttttcca aaccagctac gcttccccat
    11281 tcagaacgtc ctgaactaac ggattgagtc aaaggaatct cctcaatcgt tactggataa
    11341 ttttctacaa gccagctaat gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa
    11401 taaatcagtt gtcctttatt ttttagcatt tttattgctg aagacaaaat ctcttgctga
    11461 cgtttttgac agtatagcgg agattcttct gtccactctt ttattgcatt tggatctttt
    11521 cggaacattc cttctcctga acaaggagca tcaaccacga tccgatcaaa aaagcccgaa
    11581 aaatgaggga caagttctgc gggtgcatga tttgtcacta ttgcgttcga aactccccat
    11641 cgttctatat tttctgataa aatcttcgca cgtttcggaa agatctcatt cgtcactaat
    11701 aaccctttcc ccttcatttg agcagctaat tgggttgatt taccaccggg agccgcacac
    11761 aaatccaaaa ctttttcacc gggttttgct gcagcagctg ttccaacgat cattgcactt
    11821 ggttcttggc tatattcata cccagcttga tggaggaaag actttccatt gacagtgcca
    11881 agaaatccct cattgctgta gggagcgggt tgtaattctt cgctatggta agtttggacc
    11941 atgtccaatc ctgccggttt tagtggattc caccgaaatc cttttttcac gcttccttgt
    12001 tcaagcgcag aaaaaaaatc ggatgcttct tcgcctaaaa gaagacgata ttttttgata
    12061 aattgctgtg gtaacgttgt cgcttcctcc ttcattccaa tgtttcgtta gacattttac
    12121 aatctttttt acgaaaaata aaggtggctg atctactttt tcatgttgct gcctaaggtg
    12181 actgttcctt taagatattt tccaagtatt catccgtcac ctcgttttta tcccaaacag
    12241 cgttcatcat ttcaagaaaa tcttgagaag aatcatctga gcgcaaactt cccaatgcat
    12301 taagccatac caattcaagc gacttttctt ctttgatctt cgtgtgacct ttgacatatg
    12361 agaaaaatgg ccgcttaaat agcgaatcca tgtctaaatc agattttgca atgatttcac
    12421 gaatactttc atggatagaa aaaaaatctt tcaataagta gacaatgctt aaaacatcat
    12481 cggaactgca ttcagctaag tagttttcaa aataaacagt gatttcttct aatatcttct
    12541 cttttaaccc gctgattgat ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga
    12601 actctgctaa ctctcttgca gtaattgact tcattccata ttcttccacg tataccatac
    12661 ttttgaacag caaatattc
//
LOCUS       DARDHA010000078        12536 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000078 DARDHA010000000
VERSION     DARDHA010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.079"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="SDA10_001718"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="SDA10_001718"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEG4240623.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="SDA10_001719"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="SDA10_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEG4240624.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="SDA10_001720"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="SDA10_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEG4240625.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="SDA10_001721"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="SDA10_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEG4240626.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="SDA10_001722"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="SDA10_001722"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEG4240627.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="SDA10_001723"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="SDA10_001723"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEG4240628.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="SDA10_001724"
     CDS             complement(8053..8523)
                     /locus_tag="SDA10_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HEG4240629.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="SDA10_001725"
     CDS             complement(8569..8994)
                     /locus_tag="SDA10_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEG4240630.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="SDA10_001726"
     CDS             complement(9061..9330)
                     /locus_tag="SDA10_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEG4240631.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            9466..10545
                     /locus_tag="SDA10_001727"
     CDS             9466..10545
                     /locus_tag="SDA10_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG4240632.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            10766..12118
                     /gene="brnQ"
                     /locus_tag="SDA10_001728"
     CDS             10766..12118
                     /gene="brnQ"
                     /locus_tag="SDA10_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEG4240633.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         3237 a         2767 c         2349 g         4183 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttag
     9361 gcggatttta taattaactt agccatttgg aaataaaaaa acgccacgtg aattccatgt
     9421 tatattgaag ttaccacaac tctcaataga caaggatgaa ttcacatgac gcaaaaccat
     9481 gataacacag tttcacgcaa gggaaaacac ctaacctact cagaacgttg ccaaatcgcc
     9541 atcctgaaaa aggaaaacta ttccaaccgt caggttgcgg atgtcctggg ctgcgtgccg
     9601 cagaccataa acaacgaaat caaacgcggg accgttactc agcttaaccg ccagaatcag
     9661 aacgggaagg tctacgacta ttatcatgac gtctacgacg ccgatgccgg gcaggcggcc
     9721 tacgagaaga accgcttgaa ctgcggcaga cggcccttat gggccgacac ggacgccttc
     9781 attgaatggg cggacgacat gatgctgttg gaaaaatggt ccccggacgt ggtggtgggc
     9841 tttgccagaa aacacgaatt gttcgacccg gccattatcc ctgggaccac caccctctat
     9901 cagtggatcg accgtggcat catgaaaacg aagaatatgg acctgctaga aaaactgtcc
     9961 cgcaaaccga aatcgtcccg tcatggcaac cgcccgaata aacgggtgct agggaaatcc
    10021 attgaagagc gcccggaggc ggttggtacc cggaaaacct tcggccattg ggaagtcgac
    10081 acggtcgtag gaaacaagac caagtcggat gccgtgctgc tgacgttggt ggaacggcag
    10141 acacgcttcg aggtcattct gaagctggcc gggaaggatg ccgaatcggt caaccaagcg
    10201 gtccgacagc tgagagaacg ggctggtgcg tacttcggcc agctgttcaa gacggttacc
    10261 tcggacaacg gctcggagtt cgcgggactg cacgatgcct tgcaggatgt ggcaaacatc
    10321 tacttcagcc acccctatgc ttcctgggag cgcgggacga gcgaaaacca acacaagctg
    10381 attcgccgtt tcatccccaa gggcagccca atcggcaacg tgagcgaaaa ccaaatcttg
    10441 cggattcagc gttggatgaa cgattacccg cgaaagattt tgggctatgc aacgccgcat
    10501 gacacgttcg tccaggcatt taggcaggaa cggttgacgg cttgacacgt gggcccttga
    10561 caacgagcct ccctcggcat ggcttgtgtc tggaaagctg gccggggcca gccagtcggg
    10621 agactaccat gcctcagctc attgtcaagg gcccactcga ttagaaaaat tagacggttg
    10681 gtggtaacga gcaaccgggt caccatgggt ggctaactta aacttgaaat ttacgacttt
    10741 taaaaaaacc gtaaaggagt tatgtatgac aaaaaaatta tcatttaaag actatctttt
    10801 tataggctcc atgcttttcg gtctattttt tggtgctgga aatcttatct ttcccgttca
    10861 tttgggtcaa gaagcaggcg ctgctacctt ttgggcgaat ctaggtttct tggttaccgg
    10921 tatcggtctt cccttcttag gggtgattgc cattggtgta tcgaaaagct caggtgtata
    10981 tgaattagca aaacggataa atcaaacata tgcgatggtt tttaccatct tgctttactt
    11041 agttatcgga ccgttttttg ctttacctcg ccttgcaacc acatctttcg agattggttt
    11101 agcaccattt atcgatccta gcagacagac gctatatcta gcaattttca gtattgtatt
    11161 ttttgtgctg gcttggtggt tttcaagaaa accgacaaag atattagatt atgttggaaa
    11221 gttcctgaat cctgcatttt tggttttatt aggtgctttg cttctccttg cttttttgaa
    11281 tccactaggt tctgtaaatc aagcgcctat tcagccaaat tatcaagagc atgctttttt
    11341 cacagggttt actcaaggat ataacacatt agatgcgctt gctgctttag catttgggat
    11401 catcattgtc acaacaatcc aaaacatggg tgtaacaaaa cctgctgaaa tcgctaaaga
    11461 cacgatcaaa tctggtgcga tcagcattat attgatggga atcatttata ctttgctcgc
    11521 ttatttaggt gctatgagct taggtagctt tgctctaagt gaaaatggcg ggatcacgct
    11581 ggctcagatt gctgatcatt atttaggtac ttatggcagt atcttattag catttatcgt
    11641 tatattagct tgcttgaaga ccggtattgg tttgattaca gcttttgcag aaacattcac
    11701 agatttgttt ccgcaaagaa attacgcatt ctttgttgca cttgctagta tccttccttg
    11761 tttagcggca aacgtaggct tgacgaatat cattcaattt tcgttgcctg ttttgatgtt
    11821 catctatcca ttagcaatga cactgatttt actagtgtta gttggtcctt tgtttaagca
    11881 gcgtacggct gtttatcgaa tgactactta ttttacattg attgcttcga tttttgatgg
    11941 gttgaacgct tgcccagaaa ctatcaaaca aacacctatt gttcaaaatc ttttatatgt
    12001 tgcggaaagc tacttgccgt ttttcaaatt ggggatgggg tggatcgtac cagcagttat
    12061 cggttttgtt atcggactga tttggagttt cgctaagaaa gaagaagtag cagattgata
    12121 aaagaggttg tgaaagagac gttcagcctt gagcaataaa aagtaatttt gaaaaatggc
    12181 ttctcacatt tttgcataaa tttgacttaa taccgagaaa ctgacttttg aacaccgttt
    12241 attgagaggt cgtgaaaaag ccgcttagtt tcaagtatta agaaatattt tgtgaaaatg
    12301 gcttctcaca tttttgcata aatttgactt aataccgaga aactggcttt tgaacaccgt
    12361 ttattgagcg tctataaact gacctgagta ttagagaaac aaaacggcaa agtagctcca
    12421 tatactttgc cgttttgttt tgaactaata ccgaagagaa gctgatcaaa caacatttct
    12481 tttataatat agattttaga catatatatg atataaaaat agttttcgca aaaaaa
//
LOCUS       DARDHA010000079        12413 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000079 DARDHA010000000
VERSION     DARDHA010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12413
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.080"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..273)
                     /locus_tag="SDA10_001729"
     CDS             complement(<1..273)
                     /locus_tag="SDA10_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEG4240634.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTI"
     gene            complement(270..2459)
                     /locus_tag="SDA10_001730"
     CDS             complement(270..2459)
                     /locus_tag="SDA10_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4240635.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            complement(2593..3783)
                     /locus_tag="SDA10_001731"
     CDS             complement(2593..3783)
                     /locus_tag="SDA10_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HEG4240636.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            complement(4655..6286)
                     /locus_tag="SDA10_001732"
     CDS             complement(4655..6286)
                     /locus_tag="SDA10_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEG4240637.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(6300..7973)
                     /locus_tag="SDA10_001733"
     CDS             complement(6300..7973)
                     /locus_tag="SDA10_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEG4240638.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(7986..8303)
                     /locus_tag="SDA10_001734"
     CDS             complement(7986..8303)
                     /locus_tag="SDA10_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG4240639.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(8328..9728)
                     /gene="lacG"
                     /locus_tag="SDA10_001735"
     CDS             complement(8328..9728)
                     /gene="lacG"
                     /locus_tag="SDA10_001735"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEG4240640.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            9965..10750
                     /locus_tag="SDA10_001736"
     CDS             9965..10750
                     /locus_tag="SDA10_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG4240641.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            10753..11445
                     /locus_tag="SDA10_001737"
     CDS             10753..11445
                     /locus_tag="SDA10_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG4240642.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(11719..12090)
                     /locus_tag="SDA10_001738"
     CDS             complement(11719..12090)
                     /locus_tag="SDA10_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEG4240643.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         3804 a         2571 c         2063 g         3975 t
ORIGIN      
        1 tattgtcccc acccaaatat taatatcatc atctatacta agtaaactta ggcgttcctt
       61 aatttcttca gcagctctat tagtgtatgt gatagcaatt attcttttta gtgatccatc
      121 gtgtttaatt gcttggtaag caattttcct tgtaagtacc cttgttttcc cactcccagg
      181 acatgcattt acataaaggt tatcttcact ttccacaaca tctttttgtt ctaaatttaa
      241 atcatttacg attttttcga agatatttgt cattttgaaa cctcagcata aagtattttt
      301 gtgtaatttt ctatctgatt gattagatac acaacatcct catctttacc aaagtactct
      361 aataaagaaa ttattgctat attcaaatca gctgtatcac ttgtttttaa actagctgat
      421 aaagctgaca attctgtagt ttcctcgtac aagtcgattg tatggagtag aattttttta
      481 taaattgaga gatctactat ttcctgacat gcaaaagcaa tagcattaag aatatactta
      541 ggaattttga ttaaaaagtc aattttttca cctaactcaa ttgcaaacca ccccttacca
      601 acatgattgg ctagttttaa catcgctaaa tttctttcta aatattgttc actttgtatt
      661 tcttctttat aagcagttat tgttttttta tctttgaatg cgtcatctat aattggactg
      721 acataagtta gaatattttc tttattcaca tctaaaaact caatttcaaa tgttgtatta
      781 gcataaaaac aattaaccca attgttttca ttaaaaaggg aggataattt ttcttttctt
      841 gcttgacctt tctcttgtgc agtgctttta tacaatcgat gttctgatgg gattgcctgt
      901 tcatctaaat cagttacaat agcacacttt ctctgtattc tatcatcgtc aaacaagcta
      961 gcgatatatt caaacgaagt actaccaaca ttaatcaaac ctattccaag ttcgtctaag
     1021 tttataccaa ataatttttt tactatttga ggaataagta tttcttcagc atctccttca
     1081 actaagataa cacctttaga aaaaagcaat ccatttcttt taacatctaa atatctctct
     1141 actgcgttta ttagatttag attgttcttt tttaactttt ctttagcaaa tgagttcaga
     1201 ttatttgctg gattcataac gatagatttg ccattaaacg attttaatac attcatccga
     1261 gatatttcag acacttcagc taaatgtaca gaatgtgtgg tcatgagaat ctgagtatag
     1321 tttttttgaa gatttaatcc ttcgaacaga gttttttgaa tatgatgatg aatatgagct
     1381 tctggttctt caatcaacat aatattaagt agttctcttg aacgacacgc ttcaaattcg
     1441 actattttca aagctaaata aatcatattt agatgtccta accctagtaa gtctaagtcg
     1501 atattttgtt ctggcttcat tgaaagaaat tttgctaaag aacccatatc atctgacata
     1561 gttgaggaaa gaagaagatt aggagaatac actgaaccaa ttatgctatt tagttgccga
     1621 ttaatatttt cgccaatttt cctaatttca ggaacactgg taatagtctc atttaattgc
     1681 tgtataattg tttttaatga ctcaacatta tctgatgata tttttgattc aattgtttcc
     1741 atgattcttt tgactgggtt tcgattgttt ttcatttctc taagaacatc tcgaagagcg
     1801 tcaacataaa caaccgaaat atgcttgaaa atatctgaca tgtcaattct tgagcccaac
     1861 agagcttcat cgtcttcttc aggattacta gctatattgt cattgagtct tcctaccaat
     1921 ttatcataat tttcattgat acagaaattt agatttgatt tagatgtgta gtaaaattca
     1981 tagtcggata gacgaataga atttctaaat tcgttaaatt gatgagagtc taattcagtg
     2041 gaaatatcaa ataattgctt tctaatagct ttgttaggtc taataaatag agaaatacta
     2101 cctacacctt tatctgcatt tgaaatcaat tcttctaaga ttgtttgact ttcagaattt
     2161 aaagaaatgg aagcacaaat ctcattgtca aattcttctt cgctgattcc ttcaaatgtc
     2221 gcagagataa taatccaatg cccttgccac ccattttgaa ttccatgaaa aaaatctgat
     2281 tcttttagtg ttttactact atagtaataa ctatcatcta ataagattct caatgcagta
     2341 agagcattgg ttttccctga atcattctca cctataatag tatttgtctc attatcgaat
     2401 cgaaagattt cggattcata atttctaaaa tttcgaattt gaagagtaga tataaacata
     2461 agcagatctc ctaactattt aattaatatg attatatagt aagtttatca taaattatgt
     2521 tagtaaaagt tctcattata agggagcgtc aataattttg tgtaaatgat tcttccctac
     2581 tgcagattgt ttctaattct caatcagttg gcttagcatt tgttcgagct cgccttcggc
     2641 ctgttggaaa cctttatgac tgcgacctag gaatttttgg ttgtaagtat caaaagacga
     2701 gactaagaaa cgttccatcg actcttcatt ttggaactgt tccttgcggt ggctgtattt
     2761 cttgatttgt ttattaaagg attcaatcaa gttcgtagaa taaatactct tgcgtatggc
     2821 caaggggaaa tcatagaaag tgagcaagtg atcgttcgaa agaatcgatt caaccacttt
     2881 tggatagctg gttttccatt tttccgcaaa agtaccacgt gcttccaacg ccacctcttt
     2941 agatgaggct tgatacacca ttttaaaatc atcacagact tccttacgat catcgacacg
     3001 cactttatga ctgatattac gggaaacgtg tacacaacaa tgttgaaaac gagctttggg
     3061 atagaaccga ctaatcgctc ctaccatccc ctttaagcca tccgtgatga acaggaggac
     3121 atttttcaaa ccgcgctctt gaaggtccaa taaaatttcc tcccaaatcg tgatggattc
     3181 agtcggtgca atcgcatagc tcaatacttc ctttgatccg tccgggcgaa tgccaactgc
     3241 gatatgaata gcttccttgg cgacggtttt ccgctttaac ggaatatacg ttgcgtccat
     3301 ataaatagca gcataacggt catgaagctc ccgcccatta aacgccgtta cctcttctgt
     3361 aaatgatttt gttatattgg acatggtttg gggcgtgtag taatgcccat acattttctc
     3421 aatcaagtct gcgatttccg acatggtaat accttttcgg aagaggtgaa tgacggtctc
     3481 ctctaacgtg tcattcgtcc gtctataagc aggaacagtc tgttgcttga actcaccatt
     3541 gcggtcgcgc ggaatctgga gatgaagttc cccatactcg gtcttgaccg tacggtcata
     3601 ggagccgtta cgagaattac ccgtgttaaa accaatgcga tcatactttt cgtaatctag
     3661 gaaagccgtt aactccgttt tgagaagcgt attgactgct ttctccaaat ggacacggaa
     3721 taattcattc aaatcgcctt tgttcgctag agtctttaga atttctgtag taaaatcgtt
     3781 catagggaag tcctctcttc tgtgaatgtg ttgtggtaac tctattctac agaagggact
     3841 tccttttttc tatttacaca aaatatttta cactctccat tataacaatt gggttaaaaa
     3901 gaaaatagat agtattctac ctcatattta tttgaaagta atatataaaa gacgttcaaa
     3961 ttcgaacgcc tctcgttaac caaatattgg attcagaatc tagttttcat atataaacag
     4021 agtatgatta ggtgcaaaaa ttgcactttt attgcacttt tatttttatt tatagtgttt
     4081 tactgttctg tatattgttt taactccctt gatagtatcg cattctgttt tatacacttt
     4141 acgttgaaaa cagagtggga ataaagagcc aacataatat ggagggggaa tccctataaa
     4201 tcaacattct tcaatagcta aaatgcttga ttttcattaa aaataagatg gttccctacc
     4261 aaaagagact ataatataag ttaatttgtt tcattcttat cataaaaaga agttctcgaa
     4321 aaacgaactg ttttccgaga acttttttct atccatctaa gtgctcaaat catattcacg
     4381 acggaattct aaggttaccc tttcttaaac aaactttatg ttatctgcac aactttttgc
     4441 aattttttca ttgcttctta cgaaatcgtt gaactctctt tagcataata caaagtgtag
     4501 atgaagtctg gtgctttact cgttttggta aaaatgactg gaaatcgttt ctggttattt
     4561 ttggcaaagg cgaggtgcag gttcgattcc tgccttctac gctaacgatg tggctgaagg
     4621 gaacctccga tcttgaatcg aaacgatccg cagtctattg tttcaccaat gaaacaaaat
     4681 caacgttcgt ccaagcattt cctgaagcct tgctataaac cccaatttca caaacacctg
     4741 ttgtaattgg aatgtctttg attgtaattt ttgtccattc acttgtttta acaaccgcag
     4801 tatttagttc cgctccacca taatttttga tatataactg attagtatca aactctcccc
     4861 cagacattac ataggcactc aatgtgtaag ttcctttgct tatgccacta acattttgat
     4921 aagctgatgc ctcgtatgct tgatcctgcc aaaaagttag tttgtagtcc ccaaaaaaag
     4981 tgcccgcttc cgttttgatc gtacccttag cggttccttg ttgaaaccag gtttgccaat
     5041 tggaaggact ggtcgttggt ccatcgcttt cgaaacttgc atttttaact agatttattt
     5101 gcgcccctgc ttgtttaaac gcacgcaagc ttggcaaggc gtttccagca aaatcaaaca
     5161 tcgcttggtt gtcccattcg tttcccatag tggattgaac actgagataa ttcatacctg
     5221 cttgtgttgc ccaactcaca tttccgttgt accaaagagg ttcccaatag aagaagcccg
     5281 ccccgcgatt attcggtact gcctgaatcc gatcggcaag atccagcaaa tactctgctt
     5341 gtcctgtcgg tgttggttca tatccccccg cagctgcttc ggcttcacca aaagcatttg
     5401 gcatagtatc ggcattcgct gttgtatgcc cataagctgt ttcaactacg atcacttctt
     5461 taccataccg ttggctgatc gcattcatat tggtttgaag ttcattcatt gaaccgtgcc
     5521 aatacggata atacgacact ccaattaaat caaatgacac acctcgactc gtgatttcat
     5581 caaaccacca agtaaatgct cctgtatcgc caccatcagc taagtggagc attactggag
     5641 ttgttttagg ttgagtgtct cgaacagctt ggataccaga cttcaaaaaa gcagccaatc
     5701 gatcaaattc tcccccgcct tctccccaag acttgccata aggccagagc attccgctat
     5761 tcagttcatt gccgatctga accatatcag gataaacgtc cagctgcttc atttccgaca
     5821 gaacgttctt tgtatagtcg tacaatgccg tattcatttc ttcaaaggtc agaccttgcc
     5881 atgctttagg aagattctgt ttcccaggat caacccaaaa atcactgtag tgaaaatcca
     5941 gcagcacctt aagtcccaga ttttttgctc gttttgcaag gtcaatcgtc gtttccagat
     6001 catttgtccc tgctccataa ctttctccat tactattata aggatcctgc cacaaacgca
     6061 gtcgaacata gttgacacca ttatttttta aaatctctaa ggcatctttt gaaatatcgt
     6121 cttcataata ttttgcacca gatttttcca tatctgacag gatcgaaata tctgctccgc
     6181 gaataaattc gctggcagat gcttcaacag tttttgaaac agcccccaaa gcccctaaca
     6241 cagctgcaaa taaaacagct ttacgcaacc aaattcccat tttcattatg gctcctcctt
     6301 tattgaaatt ggttcagaat aaactgaaca gctttttcag gatctctcgt taatccgata
     6361 tattctgctc cttttgtcgc aaccagcttg attcctaagc gatcggtgtc ttctttgata
     6421 tcatcatagt aagaattgac ttgcggtgct aagaccacca tatcaaaatc ctgcagcatt
     6481 tcgtaatgag aaccataagc acccgccatt gcactgatcg gtgctttcga ttcagatgca
     6541 ccttttttta aagcattagc taacatcgca cttgtccctg cgccagcaca aagtaccaac
     6601 acattgattt ctttatttgc atcgctgata acttgactcg ctgccacagg tacttcctca
     6661 cgtacatcgg cagaagcacc ttcctcggtt gcgatcacct ctgctttttg tgcttctttc
     6721 tctactaaga cagcatcgta tgctttgcaa aatggtagat agatcaggaa gtcaacgacg
     6781 attagaaccg ctgctagaac aaaagctaag atacccatgc ccgttcctag aaccaaaccg
     6841 atcggtgctg gagttgccca aggcaaaacg tacataaagc tgttcatgcc caatacatcg
     6901 acaaaaaact tgaacaggca aacgtttaca acaggtgtta acaaaaatgg aataaaaaaa
     6961 taagggttca aaatcatggg tgccgcaaat agcaatggtt cgttgacagc gaaacttact
     7021 ggaataaagg aagctttccc aactgccttc aactgcttag acttagcaaa gagtaggaag
     7081 ataaaaggaa caaccaatgt tgccccagtt cctcccatag ttcctacaaa attccctaaa
     7141 cccaccgtca gtactttaga agcttgttca ccggaattga acaattgcag attggtttcc
     7201 acattggcat agataattgc cgcaattgcc ggctccacga tcgacggtcc atgaacaccg
     7261 acaaaccaaa acagcgccat cgctccccag ataatagcga ttcctaagta cccgtcagct
     7321 gctgtaaata gcggctgcag caccgtaatg attgcttctg caaatgagat ttggaagatt
     7381 tgtcgaatgc ctaaatcaat taaaacggcc gcaaatactg aaaaagagaa aggaaaaata
     7441 tctcggaagg tttgcgaaat cgttccagga acttctttag gcattttaat ggtaatatct
     7501 ttcagcacac aaaacttata taaattaact gttaaaaatg cagcgacaaa ggaagccagt
     7561 agtccctttg tccccatata ttccgttgaa aatccacctt caatcggaga gacgcttaac
     7621 aacaggaaac ttaccacaga ggccagcatc acagaggtgg tgttgattac tttgtttttc
     7681 ggtaagcgac gattcatcga atcagccaag cagcgagcag tggttgctga aacgagtaac
     7741 cctacaacac ccatcgaata attatagact ttccatagcc atgtggagac agattcaggc
     7801 cattggtaac caaaaatctc aggaaccgca gcaatcaaaa tgaaaatgct tgaaaataaa
     7861 atagctggca tcgctgttaa aaatccgtca cgaacggctc ctaaataact attacgtgag
     7921 attttttcaa aaaatggttt ccctttttcg atttgtttaa tgattccatc catagcgttc
     7981 ttctcctatc cttgattctg tctgtacagt acaataaagt cttgaatcag atctctcagc
     8041 aataaagtgg tcattaaatg atcctgccca tggataaaaa tgaatcccat ctccatctct
     8101 ttcccagatg cttcttccgc taaaattttt gtttgcgaat tatgtgcaag ggttaaattt
     8161 tcgtcggctt ctttcaacgc cgaatcaaca tgttcaaagt ttccttgtct tacttctttc
     8221 aataatttca ataatgtact gcgagcgtcg cctgaatatg caacgatctc aaaaccaagc
     8281 atttgtaatt cctctttatt catcttttct tcctccatta tcacttatta aataatcgtt
     8341 ttggtttcac tgaccaactt gtaccagtaa gctgattctt ttgggtaacg cttctgggtt
     8401 tcaaaatcaa cataaaacaa gccatacctt ttgttatacc catttgtcca tgagaaaaga
     8461 tccataagcg accacaaaaa ataccccttc acatttaccc cggctgttat cgccttgctt
     8521 aaggattcca aatagactct caaatagtcg attctcggct gatccataat aatgccatct
     8581 tcaaactgat ctttataccc catcccgttc tcagtgatat agattttgtt gtaatgtggg
     8641 taatcgcttt taattcgcag caacaagtca tatagacctt ctggataaat cagccagtcc
     8701 caatcagttc gaggaattcc ttctttataa atacgttctc cgattccctt cactttataa
     8761 acagaggtcc ctttttcacc cgtaccgtta tgatggattg cattttctcc atcgtaagct
     8821 ttgacaaaat ggcattggta gtgattgatc cctaagtagt cattacgcgt ggatgctttt
     8881 ttcaattcaa cgaaatcttc ttcaggaaaa tgatagctgg cctgatttgc ttcacatatc
     8941 tcatcaagag cagtcaaggt ttcagttgaa taatagccta aataggtagc atctaataag
     9001 aaacggatcg acaatgcatc gtctaaaaag gcagcatgct tatcttccgg cgcgtctgtt
     9061 gccgcatatt tcgtctctaa tgagtgaact acaccaattt cacccggaag ttcgttttct
     9121 ttgaaatagt tcactacacg ggcatgagca accatcatat tatgaagaca ggcgacgatc
     9181 tttgtaaaat catatttgat tcctggtggg aaaacaccta acaaatattg attggttgca
     9241 actggataga tttcattaaa ggtactccac accttgactt ctttgaattc atgaaaacaa
     9301 aaaatggcat aggaaacaaa tgcttctatt gtttctcgat tcaaaaaatc tccatggtca
     9361 aacaaacgtt taggggtatc aaaatgatgc aacgtcacaa acggcgttac gtttcttttc
     9421 ttacattctt cgaacacacg atgataaaaa gcgattcctt ctggatttgg tttcccagct
     9481 ccatcaggaa aaatgcggct ccaagcaatg gataatctta gaccatttac accaaaacgt
     9541 tcacacaatt caatgtcctt tgcatattga tgataaaaat cacttgccgg atcgggactg
     9601 aatcgccctt gttcctctaa aaagtcatcc caagcaacag cgcctttgcc accttcctta
     9661 gtggctcctt caacctgata ggctgctgtg gctccgccaa aaataaaatc ttctggtaat
     9721 ttaagcatgt acatctctcc tcaaatctat aatcagtttt cgctctttct aactgattgc
     9781 attgtaaacg catccaaaac atttgtcaac aaaaacaaac acaaataatc ataaaaacca
     9841 gaaaacagca ataaaatcta ttgtaacaaa cataaaaacc accctaaatg tttacttttg
     9901 ttcgaattag attgactgtt gtccgtttat atgatagatt atccttaaat tagaggtgaa
     9961 gacaatgttg aaaaaagaaa gattgcttac tatcgtagaa atggtcaata aaaaaggaat
    10021 ccttaccgtt aatgaaatca tcaatcaatt agatgtttct gatatgaccg ttcgcagaga
    10081 tttagatgaa ttagaaaaat cgggcaagct gctgcgtgtc catggagggg ctcaaagtct
    10141 ttcctacact ttagatcaag aactttctca cactgaaaaa gccactctgc agattcaaga
    10201 aaaaaaagaa attgctgcca cagcagcctc cttgattcac gagggagaaa cgattttctt
    10261 aggaccaggt acaacaatcg aactattggc gacgcattta ctgaacaaga aaattcggat
    10321 cattacaaat aattatccag tttttgatat tcttgctcaa gcagatggac cagaaattct
    10381 actgattggc ggtgatttcc ggaaaaatac cggcgcattt gtcggaccga tcaccaacga
    10441 caatttaaga agactaaagt ttacaaaagc atttatcagt tcaaatggcg ttcacaacga
    10501 agcgatctct acttacagta ccgaagaagg agaagcacag cagatcgctc taaataattc
    10561 ccgtaagaaa tatctactcg ttgataataa aaaattcaat cgagatgatt tttatgtgtt
    10621 ttataatttg catgatttcg atctgctcat tacggatcgc cagatctcgc gggaggttca
    10681 agagcactat gcacaatatg tcgatctgca aattgctgat tcaaccgaaa ataccactat
    10741 aggagcgtaa ctatgaaagg gattatattt gatttcaacg gaacaatgtt tttggactct
    10801 catctgcatg aagctgcttg gctgcatatg atacatgatc atacaaaaga tggtttatca
    10861 gacgaggata ttttaaaaaa tattcatggt cggacgaata cggagatttt gaaacatttc
    10921 atttcagagc atttgaccgc ctctgagatc gccgtattgt cagaagaaaa agaaagctat
    10981 tatcgtatgc tttgcttgca aaatgaaaac gagcttcagt tgacaaaagg gcttgaagcg
    11041 actcttgacc agctgactga ccaacagatt ccattaacga ttgccacggc tactgtcaaa
    11101 gagaatgttg ctttttattt tgatattttc gatttagccc gctggtttga ctgggacaag
    11161 attgtctatg acgacggctc tttccctggg aaaccgcaac cggatatttt tcttaaagcg
    11221 gcagacaaac tgagcttagc tccggctgat tgtcttgtta tcgaagatgc ttactcagga
    11281 ttacttgctg ccaaacgtgc ggggatcgga acaattattg caattgatcc attcggtaaa
    11341 aacaaaacag tatttgaaca ggcacagcta aataaagatg gaaccatcaa agattttact
    11401 ggtttttgga cagagcacct gtctgccaca tccgttaatt cttaaacgat tcaactaact
    11461 accaaagaaa agcaactgaa atgcacacct ccttttataa aaaactgcca gtgaaacgac
    11521 atgaaagata cactgctata tccttagtga aatatagcag tgtatctttg actttttcat
    11581 tcttttaacc atatgacgcg ttcatctcta tctataatta acattaaaaa tagtgttttc
    11641 acattatttc tcaaaccaat cttaaaaatc atcaggagta cttttttgat atacttttat
    11701 taatgctggg cgtaatggtt aaaagatttc cactagttcc tctccaccaa gctgagttga
    11761 aaaatgctta tctgtaaaca acatagacaa agaggaaata aacgtttccg tatctttctc
    11821 tgtcccaagg atattaatta attttcctac taggtaagta tcattaaact taagatctgc
    11881 cttcacatca aaagttttga taaaagcttt tatcatgcgt acaattttgt ctttatcgcc
    11941 ataattgatt tcaccactca tatcaatata gtcactaatc cctgaagaaa taatggcaac
    12001 tgcttcttct ttcgccttat cagataattc atttaatgac tgggattgat tgatgacttc
    12061 ttgagtttta tctgttacgt aatctctcat tatcctatcg gcatactccg caaagtaatc
    12121 ttgaaaaaat tcttctacag ggtaaccaac ttcgttttcc caaccaggag aaatttcatt
    12181 atcagtactt attaaatcta ttaaaaacca acccgttctc tgagtgttga ttctataata
    12241 tttttaattt cctatataaa tcaaaaatat tatagaatca ataagctaat taagaaacaa
    12301 actttagaaa ctctagaaga acttctgaat ttatatagaa gtgtgtagct acttactaaa
    12361 tatacaaaag ttaaaataaa atactctgga tactctttat actgcattaa agt
//
LOCUS       DARDHA010000080        12319 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000080 DARDHA010000000
VERSION     DARDHA010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.081"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="SDA10_001739"
     CDS             complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="SDA10_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEG4240644.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1195..3036)
                     /locus_tag="SDA10_001740"
     CDS             complement(1195..3036)
                     /locus_tag="SDA10_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEG4240645.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3218..5533)
                     /locus_tag="SDA10_001741"
     CDS             complement(3218..5533)
                     /locus_tag="SDA10_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG4240646.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5718..6578)
                     /locus_tag="SDA10_001742"
     CDS             complement(5718..6578)
                     /locus_tag="SDA10_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEG4240647.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6637..7158)
                     /locus_tag="SDA10_001743"
     CDS             complement(6637..7158)
                     /locus_tag="SDA10_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEG4240648.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            7436..8197
                     /locus_tag="SDA10_001744"
     CDS             7436..8197
                     /locus_tag="SDA10_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG4240649.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8223..10814
                     /gene="mprF"
                     /locus_tag="SDA10_001745"
     CDS             8223..10814
                     /gene="mprF"
                     /locus_tag="SDA10_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEG4240650.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="SDA10_001746"
     CDS             complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="SDA10_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEG4240651.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
BASE COUNT         3472 a         2614 c         2183 g         4050 t
ORIGIN      
        1 tttttctttt aaaataaacc aaacagtttt actgatttct cttctggtca atagtccacg
       61 cttattctaa gaaatctttt aattgttttg aacgagaagg atggcgcaat tttcttaatg
      121 ctttagcctc gatctgacga atacgttccc gagtaacacc aaatactttc cctacttctt
      181 ccaatgttct cgtccggcca tcatctaaac caaaacgtaa gcgaaggaca ttctcttccc
      241 gatcagtcaa tgtatctagt acatcttcca attgttcttt tagtaactca taagctgcgt
      301 gttctgctgg acttgttgct tcctggtctt ctatgaaatc acctaagtga gagtcatctt
      361 cttcaccgat tggcgtttca agggaaacag gttcttgcgc gattttcaag atttctcgga
      421 ctttttctgt tggaagatcc atttccgcac cgatttcttc tggcgtaggt tctcttccaa
      481 ggtcttgcag caattgacgt tggatacgta tcaatttatt aattgtttca accatatgaa
      541 ctggaatacg gatcgtacgt gcttggtcag cgattgctcg agtgatggct tgacgaatcc
      601 accatgtagc ataggttgaa aacttaaatc ctttgcggta gtcaaatttt tcaactgcct
      661 tcatcagtcc catgtttcct tcttggatca agtcaaggaa ctgcatgcct cgtcctacat
      721 aacgtttagc gatacttact actaaacgaa ggtttgcttc tgccagacgc tgtttcgctt
      781 cttggtcgcc ttcttcgatt ttcaatgcta acgctacttc ttcttccgca gttaaaaggg
      841 agacacggcc aatttctttc agatacatac gaaccgggtc attgattttt acaccggttg
      901 gcgcagaaag atcttctgtt tttgcttgtt cagctttttt ctcagcactc ttcaagctgt
      961 gaattgatgg atcaccattt tcatccacca cactgatacc tgcgtcttca atcttttgga
     1021 tcaatttgtc catttcatct gcattcaaag tgaatggtgt agcaagctga ttggataaat
     1081 catcatatac aacttgtcgt ttgggtttgt tttctttgat gaaagctgct actgctgctt
     1141 catacgtttt tttatctgtt ccatttgcca taaaagaagg ccccccttga gtagctattt
     1201 ggcttgtttt aactgtttcg ttaaattgat gatttcaatc gtcaattcaa gctcaagctg
     1261 tttgtttcca gttcgtctgg cttcttgctg catttgtttt tttaatgtta tttcatcagc
     1321 taagccagat ttgctgatca catctagcaa gtcttggatt tctcgtggcg aactttcttc
     1381 tgccaccggt aaatattcga tttccaccac caaccgcctt aaattttcat cttgcaaaaa
     1441 gtctaaaaac tctgcaaggt tgaattcata atgttgtgag acatatgcat cgaataaaaa
     1501 ataaacttct gcatatgcat catgaataaa ctgaaattct cgttctttta aaatattacg
     1561 gacagaaact tctttaaatg cacgatataa tagaatctgc tctgcctttt ctacttgcgt
     1621 caaaggtctc tttttccgtg cagtttcttg tctcgtaacc tgagtgtgtt gactttcctg
     1681 aggttcttga cgattttgct gatttgtcct tcgaagatcg cgcagttgtt tttgtatcgt
     1741 ctctctgccg atctgaaact ctgttgacag ttgagataga tacatatctt gttcgattgg
     1801 tgaaacaact cggctaagtt caactagtaa ttcttcgagg tattcgatct tgtctttttc
     1861 attatctaaa tttttccctt gttttaaata acgtgattta aaagtaaaga ctgtttcgcg
     1921 cccatgagcc actaattctt ggaaagatgc ttctccatac ttacggagat attcatcagg
     1981 atcaagcttt tctggaacga ctacgacaga aagtgctaat cgactgtttt gccgaagcaa
     2041 ttcaatccct cgattggttg cttcaaaacc tgcgttgtct ccgtcatagc aaaagaccaa
     2101 ttctttcgtc agcttttcca tttgttggat ctgttgagct gttagacttg tccccataga
     2161 agctaatcca gttttgatac cagcctgcca tgcagcaatc acatccataa acccttcgaa
     2221 caacaaaatt tctccgtttt tccggatatc gcttcttgct ttatcaagat taaataaaac
     2281 ttgtctttta ttgaacagtt ctgtttctgg actattcaag tatttgggct gatctttttc
     2341 ctgccctttt tcaggtgcaa gccagcgtcc tgaaaaaccg atcgtctggc cttgagaatt
     2401 acgaagtgga aacatcactc tttgataaaa acgatcagac atacggccat catcatgcgt
     2461 gacaaacaat ccagagcgtt ctaacaattc atttgaaatt gattcattct tgaatacttg
     2521 ctgaagaaaa gaacgctcat taggtgcaaa acctatgttg aattcttcaa ttaattctaa
     2581 agacaaccca cgactttgca aatattctaa agctggctgt ccagcttttg tatgcaatag
     2641 catgtgatga tagatttccg ctgctttttc atgagcagca atcagtttcc ccgtagcaga
     2701 ttcaggattt tggacagcta tagtacgctg acgccattga tcttctaacg gtatctgttc
     2761 gaagtctgcc acttttacca ctgcttctgg aaaacttaga ccttctagtt cttgaagaaa
     2821 agtaaacacg tttccgcctt taccacaacc aaagcaatga aaaatctgct tgtcttctgc
     2881 aacagaaaaa gacggtgtac gttcttcatg aaaaggacac agccccaagt aatttttacc
     2941 agactttttc aactggacat attgtccaat cacctcaacg atattcgttt gactgcgtac
     3001 tgtttcgata acctcttgag gaatccgttg agccattttt tcaccccctg ctctattgat
     3061 atcttttggg tttaacagaa gatatttcag gagaataacc ggtaaaaaat cgcactaact
     3121 gaaatcagtg cgattttaaa ggacacaatt atacatgttt gattgtatca tatttcattg
     3181 ctttttcaag tataacacat ttctatctca ttaagcatca atttgctact cttttcagta
     3241 actgattttg aatatcagcc ttccagtaca atactcgaag aacaatattt acgagcatga
     3301 atacaaagaa gtatcctacg ctctctccca accctaagtt tacttccaaa attccatgga
     3361 acagcattgc agctacatat atccctacga tcgtcgttac tgctaggaag atgcgcaatg
     3421 ggttgaaggg ataacaagct tttacaactg ccatgcaact gatcccaatc agcaagtaat
     3481 acatcaaagt agtagtttca gcatttcccc atgcgaatgt ttttccaagt agatacaccg
     3541 caatgatatt cactactaca agcaaggcat ttggtaaagc attaataagt gctgtaggca
     3601 aataacggta agtgattttt cttttatctt gttcaaaaga taaaaagaag gaaggatacc
     3661 cttcaatcgc taaatcaatc agcgtgattt ggatagggat aaacggaaat ccaattgctg
     3721 taacggcaca tataatcgat aaaataaatg aataaatcgt tttgataaaa aagatgcctg
     3781 agactttcgt cacattgttg accactcggc gtccttcaaa caggacttct ggcaatgtag
     3841 taaaatcaga gtccaacaat accagattcg caatctgacg tgttgctcca tcaccttctg
     3901 ccatagcaat gctgcaatct gcttctctta acgccaaaac atcattcaca ccgtcgcctg
     3961 tcattgcaac cgttctgccg ctttctttca attcatttac cagcaatttc ttttgctgcg
     4021 gactcactcg tccaaaaacc gtatagcgat gagcagcttc tcgcacttcc atttcttcag
     4081 taatttgaga aagatcgaca tacgcatcat aattcggtaa tcctgcacgt ctagcgatat
     4141 tggagacagt aaaaggattg tctccagaaa tcactttcag atccacacct tcctcatgaa
     4201 gataagctag tgtttcttga gcgttttttc ggattggatc atcgatttca aacagagcaa
     4261 ccggctcaac aaatgctggt ttttcttcgt tcaattgctg atttggagcg atccccaaca
     4321 taagtacgcg atagccattc gcttgggctg tttttagact gtccggaagc cgatcttctt
     4381 ccaccaaacg ttcaggcgct cctacgtaca cgactcccag ttctggaaat tccatcgcgc
     4441 cccatttgcg atcagaagaa aatgcgactt tgtttgtcac atcataccga ttagaaagct
     4501 caaaataatc tctcatagcc cgcatagtga tgttgttatc actgctttct gccagatacg
     4561 tccccataat ttctgggatg gctttctcgt acagcggatg tagtgcttct acccgctgga
     4621 tactcatttt cccttcagta atggtccctg ttttatccaa gcataatgta tcgacatggg
     4681 ccagcgtttc aattgaatac atgtcttgaa caaggattcg tttttttgcc agtttggtta
     4741 cccctgtcgt taatgcaata cttattagca ataccaaacc ctttggaagc atgcctaaca
     4801 aagctgctgc agatgcaata acagaaattt tgatagaagc atcgcggatg aacagtgctt
     4861 ccaaaaatag tatgatcccc aaaggaataa tcacgaaact agtgaatttg gatactttgc
     4921 gaatcgaatc tacaagttca gaacgaatag ctttgtgagt ctttgcctcg ctagtcaagc
     4981 ggacagcata attatctgca cctactcgga taacttcagc catgacagaa ccgcttgtca
     5041 aaaagcttcc agaaagcaat tcagctccag tttctttgac aatcaaatca gattcgcctg
     5101 tcaaaagagc ttcatttgct tctgcccgtc cagatataac ctgtaaatcg gctggtatct
     5161 gttctcctgc tccgattttt accaaatctc ctaataccag ctctttagta gaaatcgatt
     5221 gttctttacc gtcccggatc actagtactt ctgattggga aacaatcgat aatttccgta
     5281 caagatttct tgcatgtatt tcttgataaa ttccaataac gatattgacg acaataattg
     5341 ctaaatacgc catgttggaa tacgcaccta caaataacag acaaatccca ataccaaagt
     5401 tcagaaaatt gaataacgtc aatatattat cgctgaatat ttgttttgta ctttttgatg
     5461 catcttcctg gtagtcattt tgctggccag aagccatttt ctgaactacc tcttgctgtg
     5521 taagtccatt cattttcttt ttctccattt tccgccaact ttcaaaaata atcacgtgtt
     5581 taagtctagc gtgttccaag attaaaaaca acttgttttt ataaaattta tagaaaaaca
     5641 cacgaatgat tttacaaaaa agatgccaac ctttttgttt gaatcacccg tgtgctgttt
     5701 ttattatcaa tctatcttta cttatattgt tcctccaaac ggctcatcca ccaacctagt
     5761 gctgtaccgg ctataaaaag aacaggacaa gctagtaccc cagcaactcc caatcccgcg
     5821 taatcggtag gaatgatcat aatcgttgaa gcaaaaacta cacctaaaat gaaatggaat
     5881 agcttgctat atgcctttga gaaaatgtaa tccatgattt ttgacaatcc aagaacacaa
     5941 agaatcccac caattccgat cggcacaatc acttcaagtc gtacttcttt aaatccgtca
     6001 gccatcgctt tatacaatcc catatacacc aaaaaattag aagggctcaa tcctgggacg
     6061 atcataccca aaccaatcaa tccgccggca atcagccatg ttccaaaatt ctgatcgact
     6121 tgcccgtcaa ataattgagc gccgtataat aaaaacaaat aagccagaac aaaagtaaca
     6181 accatgatga tcacatcaga agtatctctt ccttttttcc ctgcttcttt ccataacgca
     6241 ggaacagttc cagcgataca tccgacaaaa aaccataata tcgttgtttc atacgcacct
     6301 aataaaaaac tgactaaaaa agaaagtaga aagatgcctg tcactccgcc tagaccaaca
     6361 ggcagaaaaa acaaaacatt ttctttgaaa ttttttgtaa ggtgggctaa aaaagaaatc
     6421 agccgctcat acatgccaaa gacagctgct aatgcaccac cgctgactcc tggcaagata
     6481 aatcctgaac cgataaacat tcccttcaca aaccgtaaca tccagtcttt ggttgtgtac
     6541 cccacttctt gattttccat tttttgcctt ttttccacta tttgcgcact ccttctttca
     6601 tttaatttta ttcatgattg aacatttcct gctattttac ttaaagactt tttcataggt
     6661 ctctattatt ttttcattca ttttgacaat atcaggataa aacatgttac ctccattatg
     6721 atagagatag ccgccattgt aaagcaagga ctggatccgc caatatcggt attgtttccc
     6781 ttcctcattt cctaaaagtg ggctcagcac atctttagaa tacgcttctg ctaattctac
     6841 tgtgttttct ccgccatgat tttttacata ggcaatataa tctttcccaa aattatatgc
     6901 ttggacagct gtccataaat cgcatccttg tttctttgct tctactatca tttccgccaa
     6961 atatttgaca ccttgatcaa tactgtcagc tggattatca atgatctcag cttctccatg
     7021 cagactttcc gagctctgca tgagatctgt cccttttcct tttgattcgg tcaatattat
     7081 cgccttgatc aaattctcgt agttttccat atgataattt tttgcagctg ttgttatttc
     7141 tatttgatag ctttccactc gttttaccga acgataagtt cttcctcata aaaacaacac
     7201 cgcaccaata accgtcagaa tgatcatgta aaaaagaata cggataatag ataatcgttt
     7261 tcgcttcatt tcatcaccta gttattttga catctttttt tatcttacta tttctttatc
     7321 ttcttggcta tttcttcctt gatttctgtg tacgccttaa gaaaaaatga attttcctgt
     7381 attttttgac atcttacgaa aatgatcgtt gtttttcagt aaaaacaatt gcccaatgac
     7441 tgatataatc gttatacttt acacagtagg tgattatgtg aaaaaagaaa agaaatatgc
     7501 aaaaatgcca gatgggagcg aaatttacta tgaaaagagc ggtcaaggct tccctctctt
     7561 tttactgcat ggcaatgacg gcagcggccg ctttttttca gaacaggtcc ctgtactcga
     7621 acgctactat accgtgtatc tagtagatag ccgaggacat ggtcgttcga caaacgaagc
     7681 atctatgttg aattttcagt taatggccga ggatctgaat acgatcatgt tgctggaaaa
     7741 aatcgatcag gcagattttc ttggatttag tgatggagca aatctagcac tggtatttgc
     7801 cagcagcttc cctaaaaaag ttcatcgatt gattttaaat tctggaaaca cgcttgttaa
     7861 aggcgttcgt ttttctgcca gagtcatcag taatattcat tatgcatggg tatggttact
     7921 ctctttattt cgcccaagtc ttcgcaaaaa tcttttagtt atcaaacttt tgctgcatga
     7981 catcgggctt acagaaaatg atttaaaaaa aatcaactcc cccactttga tcatcgtggg
     8041 aaaaaaagat gtaatcaagc taaaacattc tttgtatata gctaaaacta taccaaaagc
     8101 ttcctttgtg cttgtgaaag aacaaggaca tgaattagct agaaaagatc ctgaacgctt
     8161 caatcgagaa gttcttcaat ttctttctga aacataacta ttaggaatag aaggtgattt
     8221 atttgttaaa aaataccata caatggctga aaaaccacct aggactgttc aaaacaatct
     8281 ttttgatttc tgtcattgtc attattgtcg gtgaattgat gtccatcggt aaaactctat
     8341 ctatccagca attaggagaa acgtttgcca ctatacctgt atggaaaagc ggactaatgc
     8401 tattgattgg tcttatctct gtgttaccta tggttggtta tgatattatt ttgaatcgga
     8461 tgcttgaaca aaagcagaat cctcgttacc tgtttgagac aagctggctg atcaatacga
     8521 tcaacaatat tgccggtttc ggcgggtttg ttagtgtggg gcttcgctct gaactatatg
     8581 gacaaaagaa agagagcaga aacgtgatcc aagcattatc gaaagttttc ttgtttttga
     8641 tggctggact ttctatttac agtctgcttt cttttttact tgtagtcttc acgccagttg
     8701 ctcctttttt gaagcaatat tggatatggt tgatcggcgg cggtctatat tttcctgtcg
     8761 tctttttatt tactactttc aaaaagaaag gattccttgg tggtacctca cttaaaactc
     8821 aaggacagct attgctagtt tcattgttgg aatggaccgg tgtcctgttt agtttccttt
     8881 ctgttggtta tttaatggaa atccggattg atctgtggca aacaattcca ttattcattg
     8941 ctgcttcagt gataggaatc gtgtctatga ttccaggaga aattgggagt ttcgatgtaa
     9001 tgatgattat cgggttgtcc gctattggtg tgccaagaga aatagtcgtt gtgtggatct
     9061 tgctctatcg cctgttctac tacatcattc cgtttttgat tggaatcgtc tttttcttca
     9121 aaaatatcgg ttccactttt gatcagcgtt attctggtat ccctaaacaa ttagcaacag
     9181 aaattgcgca taaaatcgtg gttgtcttat tatacttttc tggaatcatg ctagtacttt
     9241 ctgccactat cccgcaagct tttacagagt ttcgctggct tcatagtttg aacccattga
     9301 aatttcattt tattatccaa tttcctagta tactattggg atttctttta atcattatgg
     9361 gcagagggat cgcagcacgt gtgaaacgag catatttgcc tacaatcttc ttgattgttc
     9421 tagctttact ttatgtttta ttaagtgatt ttagtttcac accggtggtt ttcttgtcta
     9481 ttctgcttct gatcattctt gcttctaaaa acgaattatt tcgggaacaa ttgatctatt
     9541 catgggagtg gcgtacgatc gatggaatca ttattggtgc actgtccttg ctttacatcg
     9601 ttattggggt atacaatctg ccggatttcc ctcatcggcg tcaccatttt atttcctttt
     9661 tcttatttcc ttctgaaaag atttggtttt cgggattgtt ggccatcatc gctgttagtt
     9721 tcatgatcgt tctatttgtt catttccttc aaggagaaaa gaaacagatt ggcgaagcct
     9781 ttaatgaaga aaaggccctg aagatcctta caacttatgg aggaaactcc gatagtcaat
     9841 tgatcttctt aaaagataag cggatgtttg cctatgaaaa agacggtgaa ccaactgttc
     9901 ttctccaatt cgcttgcttc aataataaat gtattgtaat gggtgatcct tctggtaaga
     9961 aagaagattt tccagaagca atcgaagctt ttatcgaaga aacagatcga ctatgctatt
    10021 taccagtttt ctacgaaaca agcgaagaga ttgttatgat tttgcatgaa ttcggctacg
    10081 atttcattaa gatgggggaa gaagcttatg tagacctgaa tagttttaca acttcaggga
    10141 aaaaaatgaa gggcactcgt gctgtcttga atcggatcga acgagaagga tttacatttg
    10201 atgtcttaca accaccattt tctactgagc aaatgagtac tttcaaaaat atctctgata
    10261 attggttggg ttcacgaaag gaaaaagggt tttctttagg ctttttctct gaagattatc
    10321 tacaaagagc gcctattgca gttgtcaaaa atacccaaga agaaattgtc gcttttgcaa
    10381 cgatcatgcc tacttacacg aacaataaag taggaacgat tgatctgatg cgatatgatt
    10441 ctaaaaaagc accttctggc agtatggatt tcttgttctt gcatttgttt gcctatatga
    10501 aagaagaaca tatccaatgg ttcaatttag ggatggcccc tctttcaaat gtcggaactt
    10561 ccagaaagag ttttttacag gaaaggattg catacttggt ttacgagttt ggttctcatt
    10621 tttattcttt ccatggatta agagaatata aaagcaagta cgctacaaac tgggtctcac
    10681 gttatactct atattctaga gatagttgga ttgcatatgt tatgattgct ttgctgatta
    10741 ttgataatgc gccagttgag cagacacaag gtagaataag tggtttgaaa aaatggatac
    10801 gaagaaagta ttaaaaactg cataccgata gatgaacact ctcatgatga ataaaagaga
    10861 ctgaagtaaa acgaaagttc atgcttctac aatctcttct tatacaattg aagaacaaag
    10921 agcggtccaa acagaaatcc tgtttggacc gctcttttat tttaaacatt tttcattacc
    10981 aaaatattcg agtcgcgttt cttatgcttc gatctctgta acgatacctg aacctactgt
    11041 ccgtccgcct tcacgaattg agaatgtcgt tccgttttca acagcgattg gatggatcaa
    11101 gtcaacatca atcgtcacgt tgtcaccagg catgaccatt tctgtatctt ctggcaatgt
    11161 gattgttcct gtaacatcag ttgtgcggaa gtagaactgt ggacgatagt tgttgaagaa
    11221 tggagtatga cgtccacctt cttcttttgt caacacgtac acttctgctt tgaattttgt
    11281 atgtggagta atggaacctg gtttagcaag tacttgtcca cgttcgatat cgtcacgttg
    11341 gatcccccgt aacaacacgc ctacgttatc cccagcttcc ccgtaatcta acgttttacg
    11401 gaacatttct acacctgtta caactgcttt ttgtgtttca ggtttgatcc ctacgatctc
    11461 tacctcatca ccgacacgaa cagcaccacg atcaatacga cctgaggcaa cagttcctcg
    11521 acctgtaatt gagaagacgt cttccactgg caatagtaat ggtttatctg tatcacgttc
    11581 tggtgttggg atatattcat ctactgtatc catcaattcc ataatagcag cttcagcatc
    11641 tggatcgcct tgcaatgctt ttaatgcgga acctttgatc acaggagtgt catcgcctgg
    11701 gaaaccatat tcgctcaata actcgcgaac ttccatttct accaaatcga tcaattcttc
    11761 atcatcgact aaatcaactt tgttcaagaa aacaatcagg tattttacac caacttggcg
    11821 tgacaataaa atgtgttcac gtgtttgagg catcggaccg tcagttgcag aaacaactaa
    11881 gattgcacca tccatttgtg ctgcccctgt aatcatgttt ttcacatagt ccgcgtgtcc
    11941 tggtgcatca atatgcgcgt agtggcgttt ttctgtttca tattctacgt gtgcggtatt
    12001 gatcgtaatc ccacgttcac gttcttctgg agctgcatca atactagcat agtcttgagg
    12061 atttgctagg ccttttttgc ctaatactgt agtgatcgca gcagttaaag tagttttccc
    12121 atggtcaacg tgtccaatcg taccaatgtt aacgtgtggt ttacttctgt cataatgttc
    12181 ttttgccata atttacaaac ctcctaaata atatagatca ctgaaaaatt tcagctctca
    12241 cctttttatt atagtctttt tttgactaaa tcaaaaagaa tacgtccgac ttactttttg
    12301 taagattcga actaagctt
//
LOCUS       DARDHA010000081        12281 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000081 DARDHA010000000
VERSION     DARDHA010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.082"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(82..639)
                     /gene="frr"
                     /locus_tag="SDA10_001747"
     CDS             complement(82..639)
                     /gene="frr"
                     /locus_tag="SDA10_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEG4240652.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(642..1364)
                     /locus_tag="SDA10_001748"
     CDS             complement(642..1364)
                     /locus_tag="SDA10_001748"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEG4240653.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1500..2381)
                     /locus_tag="SDA10_001749"
     CDS             complement(1500..2381)
                     /locus_tag="SDA10_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEG4240654.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2480..3262)
                     /gene="rpsB"
                     /locus_tag="SDA10_001750"
     CDS             complement(2480..3262)
                     /gene="rpsB"
                     /locus_tag="SDA10_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEG4240655.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3621..4100
                     /locus_tag="SDA10_001751"
     CDS             3621..4100
                     /locus_tag="SDA10_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEG4240656.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4317..5408
                     /gene="phnW"
                     /locus_tag="SDA10_001752"
     CDS             4317..5408
                     /gene="phnW"
                     /locus_tag="SDA10_001752"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEG4240657.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5401..5529
                     /locus_tag="SDA10_001753"
     CDS             5401..5529
                     /locus_tag="SDA10_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEG4240658.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            5533..6078
                     /locus_tag="SDA10_001754"
     CDS             5533..6078
                     /locus_tag="SDA10_001754"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEG4240659.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6532..8223)
                     /locus_tag="SDA10_001755"
     CDS             complement(6532..8223)
                     /locus_tag="SDA10_001755"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEG4240660.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8643..9590)
                     /gene="arcC"
                     /locus_tag="SDA10_001756"
     CDS             complement(8643..9590)
                     /gene="arcC"
                     /locus_tag="SDA10_001756"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEG4240661.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9705..10724)
                     /gene="argF"
                     /locus_tag="SDA10_001757"
     CDS             complement(9705..10724)
                     /gene="argF"
                     /locus_tag="SDA10_001757"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEG4240662.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10815..12044)
                     /gene="arcA"
                     /locus_tag="SDA10_001758"
     CDS             complement(10815..12044)
                     /gene="arcA"
                     /locus_tag="SDA10_001758"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEG4240663.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3378 a         2533 c         2206 g         4164 t
ORIGIN      
        1 tttttttaat ttttaaataa aagaaatata aataaaaaca gaggaagcgg aatgatccgc
       61 ccctctgttt ttatgatttt attaaacttc caataattct ttttctttat cagcagcgat
      121 cttatctact tctttgatgc tgttatctgt caatgtttgt acgtcttttt ctaatccgcg
      181 caaatcatct tcggtaatat caccattttt ttgttgtttt ttatattcat cgatcgcatc
      241 acgacggatg ttacgaatcg cgatttttgc attttctgct tcttttttaa catctttagc
      301 taattctttt ctgcgttctt ctgtcagttg cgggataact aagcgaatga catttccatc
      361 attggttgga ctgattccga tatcacttgc cataatagct ttttcaacat cttgaagaat
      421 gctcttgtca aaaggtgtga tcattaaaac acgtgcttct ggaatttgga ttgatgccat
      481 ttggttcaat ggtgttggcg caccgtagta gttcacagta atacggtcca aaaggctagc
      541 gttcgcacgg cctgcgcgga tttgcccaag ttcacgttga agattttgtg ctgctttttt
      601 cattttttct tttgcttctg tcataattgc atctgccatt tttatttccc ccttacagtt
      661 gttccgatat tttcgcctaa gattgcacgg cgaatatttc cgtgttcatt taaattgaag
      721 acaagcagtg gaatatcatt atccatgctt agtgaacttg cagttgaatc catgacttgt
      781 aatcctttgg caataacttc cagatgagtc agttcttcaa attttactgc atttgcatct
      841 actttcggat cagcagaata aacaccgtct acattgtttt tagccatcag gataacatct
      901 gcgccgattt ctgccgcgcg taatgctgct gttgtgtctg tcgagaagta cgggttgccg
      961 gtaccgcctg caaagataac gatccgtcct ttttctaaat gacgttctgc tttacggcga
     1021 atatatggct ctgcgatttg acgcatttca atcgaagttt gcacgcgtgt cggtacaccc
     1081 acgttttcca acgtatcctg taaagctaag gcattcataa ctgtagctaa catacccatg
     1141 taatcagctt gagcacgttc catgcccatt tgtgctccga tttgtccgcg ccagatattt
     1201 ccgccgccca caacgattgc catttcaacg cccagctcgt gaacttcttt gatttcttgg
     1261 atgatttctt tgattactgg tggtttgatt ccaaatcctg cgtccccagc taaagcttct
     1321 ccactcaatt ttaagactac acgctgatat ttcggtgtga ccatgttcat tcctccaata
     1381 ttttctattg ccattttacc atacttagat gatttcgaca ctctaagact ggtttttctt
     1441 gttttgctta aaaaaaggga gcgcacaaac cgtgcgttcc cgtctgttat tcaacagaat
     1501 tattttttca cttggctcat aacttcttct acaaagttat cttcgcgttt ttcgatacct
     1561 tcgccaactt cgaaacgtac gaatgtttta acagttgcgc cttttgatgc aacgtatttt
     1621 tcaacagtca tatcaggatc tttaacgaat ggttggtcca ccaaagcgat ttctgctttg
     1681 aatttcttca agcgtccttc taccattttt tcaacgatat tcgctggttt gccttcgttc
     1741 aatgcttgtt ctgtaagaac agttttttca tgttctaatt cagcttctgg aatttgtgat
     1801 tcgtttacat aacgaggatt gatagctgct acgtgcatag ctacgtctct tgcaactgtt
     1861 tcgtcagttg ttccatctag aacagtcaat acggcaatac gtccgcccat atgcaagtat
     1921 ccaccaaatg cagcattgtc gtctttttct acaacttcaa agcgacggaa gctgattttt
     1981 tctccgataa cttgagttgc ttcgatcaag tcagattcaa ttgtaccttt atctgttttg
     2041 attttcattg cagcttccat gtctgctggt ttgttttcag caacaagttc tgcaatttct
     2101 tttactaaat cttggaacat ttcgttttta gaaacaaagt ctgtttctga gtttacttca
     2161 acgattgctg cagtgttacc tttcacagca actgaagcca atccttcagc tgcaatgcgg
     2221 tcatttttct tcgcagcctt tgccattcct ttttcacgta aaagatcaac tgctttttcc
     2281 atgtcgcctt ctacttcaac taatgctttt ttcgcatcca tcattcctac gccagtcatt
     2341 tcgcgtagtt cttttaccat tttagctgta acgtctgcca tgtttaactc ctcctgtgtg
     2401 tttttctttt taaaaaaagc tgcttcaaag gagagtgagg cttgtcgccc agcctttgaa
     2461 acagctccat cattcaaaat tattctgctg aagaattgtc gccttcaaca acatctacga
     2521 tttcttcgat tgatgttgct gtgttttctt ctgtgaaatc ttcttcaact acttgatctt
     2581 caccttggtt tccttcgatg aaagcatcag ccatttttga agtaatcaat ttaacggcac
     2641 gaattgcatc gtcgtttgaa gggattacta cgtcgatttc atctggatcg cagtttgtat
     2701 caaccatagc tacgattggg atatttaatt tgtgtgcttc ttgaacagca atacgttctt
     2761 tacgtgggtc aacgatgtac attacatctg ggattcttgg catatcagcg ataccgccta
     2821 agaatttttc aagacgttca cgttctttgt tcaaaccagc aacttctttt ttaggtagaa
     2881 cttcaaaagt tccatcttct tccattgcat tgatttgttt caaacggctg atacgttttt
     2941 ggatagtatc ccagtttgtc aatgttccgc ctaaccaacg atggttaacg aagtattgtc
     3001 ctgcacgagt tgcttcttct ttgattgctt cttgtgcttg tttttttgta cctacgaata
     3061 atgctacgcc gccttcttca gcaacgctct tcatgtaatc ataagctgca tctactaatt
     3121 taactgtttt ttgcaagtca atgatgtaga ttccgtttct ttctgtgaag atatatttct
     3181 tcatttttgg attccagcga cgagtttggt gaccaaagtg tacgccggct tctagtaatt
     3241 gtttcataga aattactgcc atgtttgttt cctccgattt ggttttattt ttcctcttct
     3301 tatcttctac cttgccggca aactttttca agcacctacc gacaatccaa taagaatgtg
     3361 gatttgctga tgcataaagc accctcagct attatacaga caaaagatcc gtatcgcaag
     3421 agcaaatata cttttctgct caacattcaa tagtttacct gaaagataaa aagaattcaa
     3481 gaaaaacagt agatttctct ttgtttcttt attatccttt ttccataaaa taattttagg
     3541 tataaaagac gaatattatt gggcaatcct tctttttttt gttatgatac atgtgaaatc
     3601 tttcgtaaag gagaaagaaa atgcgcaaag ctcaaagaca tttgctgatt aaacaagtca
     3661 ttgaagaatt cccgatccgt acgcaagaag aactattaaa caaacttggg cattatggcg
     3721 ttaccgccac gcaggcaacg atttctcgag atatccgtga tctaaaaatc gtgaaggcac
     3781 ctgatgaaaa tggcgtttcc cgcttcgtat tgtttcgcga aaaaggaact cctgagacta
     3841 ggactgagga agaggatcgg ctggttcaaa tgattgaaga cattgtattg aaagtcgaac
     3901 gagtccactt tttgacgatc gtcaatacct tgccagacaa tgctcattta tttgcatctg
     3961 ttttagatga gatcaaacct ccctctattg tcagtacaat tgctggattc gatacgatca
     4021 tggtcatctc ggaaaatgag gaggacgcaa aacgagtaga agaatttctt caccatccaa
     4081 ctgaagtatc acttttttaa tcagagcacg tattcctaat atggaatgcg tgttttttta
     4141 ttttcaatat tttccttatt gaataaacct ttacactctt tacaaaaata catcattttt
     4201 tattcaaatt tttcaacacc ttgattgaat cacaataatc atcatttata ttggatgtta
     4261 acaagagcag cttcttgctc tttgtgtatt tcaaaaaaga aatgaaagga aatcatatgt
     4321 ataaattatt aacttctgga ccattgacga cttctgaaac agtgaaaaat gctatgctga
     4381 tcgatcattg tacttgggat caagaataca aagaaattac acaatggata agaaaaaaat
     4441 tattggatct tgctcaagta tcggaaggaa tatacacaac ggtcttaatg caaggtagca
     4501 gctccttcgg ggtagaatcg gtattaagtt ctgtgatcag gccagaagat actgtactga
     4561 tttgttcaaa cggcgcatat agacaacgaa tgatcaacat gtgcgaatgt ctgaaactga
     4621 actataccgt ttatgaggta tcggaacatc aaatcctcca agctcatgtg attacacagc
     4681 tgattgaaga gaatccgtct atctccgcac tagcaatggt ccatagcgag actacttccg
     4741 gtattttgaa taatttggaa gagattgctg ctgtcacaca agaaagagat cttttgttta
     4801 tagtggacgc gatgtcaagc tttggtggga taccgatttg tgtagccgaa ctagggattg
     4861 attttttgat ttccagctca aacaaatgta ttcaaggtgt tccaggcttt tcttttgtac
     4921 ttgctaataa agcggaactg gaaaaacgaa aaaatgttag ccgaagtttg tcacttgatc
     4981 tatatgacca gtaccgcgtg atggaaaacg agaatggcaa atggcgtttc acttccccta
     5041 cccatacggt tttggctttt caacaagcgc tgaaggaact agaggcagaa ggaggaatcg
     5101 aaactcgata ttcacgttat ctcgccaata atcatgtgct acgacaaaaa atgacagaga
     5161 tggggttcca gcctttcatt caagaatatc aggggccatt catcacctct tttctttacc
     5221 ctaaggactg gcgatttgat tttaatcatt tttatcagta tttaaagcaa aatggatttg
     5281 tgatctatcc cgggaaaatc agcgatcatg aatgttttcg tatcggaaat attggcgaga
     5341 tctatccgga agatattttg gaactgacag caattatcaa agactataag gagaacgtta
     5401 atgattaaaa ctgtaatttt tgattgggca ggaacaactg tggactttgg ctgtatggct
     5461 cctgtccacg cttttagaaa tgcttttcta gaaaaaggaa tccaactgac tgacaaagaa
     5521 atccgctaac cgatgggaaa attaaaatgg gatcatatcc aacagcttct ttccctccct
     5581 tctgtcaaaa aacaatggat agctatttat ggacaactac ctcaggaaaa agaggtggag
     5641 gaattgtatc aacaatttga acattatctg tttaaagatt tggtcaagca ttctaccttg
     5701 aaacccgaca cattggaaac gatcgaacgg ttgagaaacg ctgggatcaa tattggttcc
     5761 acgaatggat ttaatgctcc tatgatgaca atcgtagcag aaaccgcaaa aaaagaaagg
     5821 tattcaccag acttcatggc aacttttgaa gacgtcgaag ggtatggtcg accgtttcct
     5881 tatatgatcc ataaaaacat gcagtacttc aaaaacaagt cggttgatga agtaatcaaa
     5941 gtcggagata cggtctcaga tatccaggaa gggaaaaatg ccggagtgtt gacagtaggt
     6001 gtaattgaag gtagttcgct aatggggatt tcttatgatg agtaccaaaa attagagatg
     6061 gggaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagcc ggcgctgata
     6121 tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac atgaatccag
     6181 aatgactttt atacaaaata agaggttgtg aaaaagctgt cctgtatcaa gtaataaaaa
     6241 cagccaagca aaaatagctc ctcatatttt tcgtttggtt aggcttatta ccgcagatgc
     6301 aagcttttga acaccgttta ttcgaaaaaa aaaagggagc tgtgacacga cttttgtcac
     6361 agtcccctta tttgcaaaca aacagtgttt aagagacaac ctctccacta aacggtgttc
     6421 attcagctga ctctcggtat ttgctcaaaa aacgataaaa tttgaaaagc tattttctct
     6481 tttgtttctc taatactcgg atcagaacgc tgatttcaca ttctcttttt cttacatctt
     6541 atctggtgca tgaagtccta gcaaacgaag gtcttctttc aataatacag tcactgcata
     6601 aactagagct aagcgtgctt ctttttgttc atcatcagct aaaatcttcg tatgagcata
     6661 gtatttgttg aatgcttgtg ctaatttgat tgcatgtttc gcaatcactg acggttcgta
     6721 tttttcccca gcagacaaga ccgtttcagg gtatttttga actaatttaa ccacttccca
     6781 gctggtatcg tcattcaatg catagtctgc tgtttctgaa gggacaaatc ctgctttttc
     6841 caacaggctg actgcacgag catgtgtata ttgcacataa ggtccagttt ctccttcaaa
     6901 tcgaacaact tcttccaggt taaagtcaaa tgtattcagt cgatcgtttt tcaaatcgtg
     6961 gaaaatgact gccccaaccc caacttgttt agctacagca tctttgtttt ccaaatcagg
     7021 gtttttctcg ctgatttgtt ctttagcaga ttggattgct tcatttaata cttcttctaa
     7081 taagacgatc tttcctttac gagttgataa ttttttacca ccttgcgtga tcaaaccaaa
     7141 tggaatatgg tgcatgtcgt cagaccagtc aaagcccatt tccttcaaga cagctttcaa
     7201 ctgtttaaaa tgatagcttt gttcattccc tactacatat agagattgtt tgaagtcgta
     7261 agtacgttta cggtacaatg cagcagctaa gtcacgtgta atgtatagtg tcgcaccgtc
     7321 tgacttctta atcaacgctg gattcaggtc ataagcagat aggtcaacga tctctgcacc
     7381 tttatcctcg ttcagcaaat gtttttcttc taaaagtttg acgatttcat ccatcttgtc
     7441 attgtagaaa gcttcaccgt tcaacgaatc gaaacgtact tccagcagat catagatttt
     7501 attgaattct ttcatcgatt catcacggaa ccattgccat aattgaatag cctctttatc
     7561 gccttcttcc agacgtttga accaagcacg agcttcttca tttaattctg gttctgtttc
     7621 agctacttca tggaattgga cgtataaacg aagcaattca ttgattggtt ctgctttaac
     7681 agcttcttct gtaccccatt ttttgtaagc gacgatcagt tttccaaact gtgttcccca
     7741 gtcacctaaa tggttgatgc ggattggctg ataaccgatt ttttccataa tgaaaccgat
     7801 cgaattaccg ataactgtcg aacgcaaatg tcccattgag attggtttgg caatattagg
     7861 agaagacatg tcgattggta ctgttccttg attcccgatg ttgctatccc cataatgttc
     7921 tttttctttc actactgttt gcaaaacttt tttgctgata agttctttat tcatgaaaaa
     7981 gttcaagtaa ggtccgacaa cttcgatttt ttcaaagttg gcagaatcga ttttttctgc
     8041 taaatctgca gcgatttgct gtggcgcttt tcggtaaact tttgctaatg aaaaggctgg
     8101 aaatgcaaca tctccgtgtt cagctgattt tggattttct agtaattgct cgacttgttc
     8161 cagtgttaaa tcatctttga cagcgtcata gacagctttt gcgacaagat ctttattatt
     8221 catctttatt cctcctaaag tgttcccttg attgttaaac aaaaaaaatc tctagcgacg
     8281 gcatacttag tatgccatca ctagagacga gatttcccgc ggtaccactc caattgtcct
     8341 aaaaaaggac cagctccatc tgatatcggt agacgaccgt ctgtttaaaa cagaattctc
     8401 caagtgcgct tcgttgtatc ttcttcattc ggcttccacc acttccgaat tcgctagcca
     8461 agaatgatac aagtactctc ttgttcaaca aatcttagtc ttattaaact ttcttgaatt
     8521 atatcagaaa aaaaaacgaa aggctagtcg tttcataaaa aaacaatcca gatggcctaa
     8581 aggagaagaa ataccacctg gatagctata aatcatttcg taaatacgaa agacttaaca
     8641 aattaatcag ctacgataat cgttccttct tcagaagcta aaagattctc gatattttct
     8701 aaagaagtga tgattgcctt agcgtttggt tttgcttcaa caaatgcaat agctgcttct
     8761 actttcggaa gcatgcttcc tggagcgaat tgcttttcat caatgtattg tttcatttct
     8821 gaaacagtaa cagtttctaa tttcttttga tctggttttt ggtaattgac atacacattg
     8881 tctactcctg tcaatacgat cagcaaatcc gcatcgatga tttcggctaa tttttctgaa
     8941 gcaaaatctt tatcgattac ggcttctcgt ccttctaatc cagtagctgt ttctacaaca
     9001 ggaatcccac cgccaccaac agagactgtg ataactcctt gatcgaccag cgtttcgatg
     9061 acacgtgctt ctttaatcga gatgggttta ggggaagcga ctacttttct ccatccacgt
     9121 ccagcatctt ctttgaatgt cacagtggag tcagcattca tttgttcttt ggcttcttct
     9181 tccgtgtaga atggaccgac tggtttactt ggatttttga atgaaggatc attttcatcc
     9241 acaataactt gagtgactaa agaaaccaca tctttatcga tccctttttc cttcagtact
     9301 tcgcccatgg cgttttgtaa ccagtaaccg atagatcctt ctgtcattgc aacacatgta
     9361 tctaaaggca ttgccggtgt tttctcagaa tcagcagctt gctgctgaat caataggtta
     9421 ccgacttgcg gaccatttcc gtgggagatg atcaattcat ctccttgttc gataaatttc
     9481 actaaatact ttgctgtttc cattaacgct tcttgttggg cttttgcact tgcatccgta
     9541 gacaagatgg cattgccccc taaagcgaca actacttttc gtttggccat ttcattcgtc
     9601 ctttcttcag ttctaccgaa ttttctagat aacaaaacga gccagcgttt tgtgattttc
     9661 cctcaatgct gactcgtttt ttcgtgagat gaatgtggtt atgcttaaac gcgagggata
     9721 aacaagttac ctaatgttgc tgccatgatc gctttgattg agtgcatacg attttctgct
     9781 tggtcaaatt gacgagcata tttgctgcgg aagacttcat ctgttacttc catttcagtg
     9841 atgccaaagc gttctttcat ttgttcaccg taaacagtgt ttgtatcatg gaaagctggc
     9901 aagcagtgca agaagatcaa acgatctgtg ttgtgtgttt tttctaccat ttccatattg
     9961 atttggtaag gtttcaacaa tttgatacgt tcttcgaatt tatcttcttc acccattgat
    10021 acccatacat cagaataaag gacgtctgct ccgtctacac ctttatcaac atcgtcggta
    10081 atcatcaatt gtgcaccgga tttttcagca aagccttctg ccattttgac gatttcttct
    10141 tcaggttgta attcttttgg tgcaacgata cgcatattcg ctcctaaaat tgcacctgtg
    10201 actaataaag agttagccat attgtttcga ccatctccgc agtaagcaac agtgatacct
    10261 tctactgttc caaagttttc ttggatagtt aaaaagtcag cgatcatttg tgttggatgc
    10321 cattcatcag tcaagccatt ccaaactgga acaccagaga attctgctag ttcctctacc
    10381 attttttgac tgaatccacg gaattcgatt ccgtcaaaca tacgtcctag tactttagct
    10441 gtatcttctg tggattcttt tttacctaat tggatatcat ttgcacctag atattcagga
    10501 tgtgcaccta gatcgatcgc tgcagtagta aatgcagaac gagtacgtgt agatgttttt
    10561 tcaaataaaa gcgcaatatt ttttccttct agatagtgat gtgggatacc acgttttttc
    10621 aagtctttca agtgttctga aaaatcgatc aaatattgta attcttcttt tgtaaagtct
    10681 ttttctgcta ataagcttct tccttggaat actgattctt tcatattaaa cattctcctt
    10741 tagattgttt ttatttaaga ttaaagagga aatcccgacg gcagctgaca gtaagactgc
    10801 cgtcaggaaa caaattactt caagtcttca cggacaagtg gttggctcat gcagcgtgga
    10861 ccaccacgac ctcgtgacaa ttcacttgaa ttgatttcta acactttgat gccatagcta
    10921 cgaagcaatt cgttcgatac ataatttcgg ttataagtga ctacaacacc tggagcgatt
    10981 gccaatgtgt ttgatccatc attccattgt tctcgaggtg caacgatctc atctccatta
    11041 ccagttggga tcagtacaag atcatctaat ccaagagcag ctttcatcgt ttcgtgtaag
    11101 ttatctgaat gagtaatctt gatatcatca ccatctggtt cgattgtaaa gacatccatc
    11161 ttaccgtctt tactttgaat tgctggatgg atagtgaatt tatcgtgatc taccattgta
    11221 aatacggtat ccaaatgcat cattgcacgg ttgttcggaa ttttgattgc caacactttt
    11281 tcaaatcctg aattttttgc gaataggtgg cgtgctaatt tttctaaagc tttcgcatta
    11341 gttcgttgag aaatcccaac agctactact ttatcgctta agatcaattc gtctccgcct
    11401 tcgatatgat ctggattttc acgatccaac catacttcga ttcctttatt tgcaaatctt
    11461 ggatgatgtt tcaaaatata ttctgtaaac atcgattcac gacgacgtgc ttcaaaagtc
    11521 atcttattga ctgtcatccc atttcccatt gatgcggaag ggtcgcgtgt aaagtacaag
    11581 tttggcattg ggtccatgta aaatggatag tcatcgtctg ctgacagatc atatagactg
    11641 ctagaacgta cttcgatatc ttttgtacga accccagcca tgattttatc taccatttct
    11701 tgcgtttcca ttgatagaag gaactcatga agaccctctc ttacagcatt cgatgcaata
    11761 tgagattcat ccaacatttt attcaagaat tgttctttga cattgcctgc gtcaatcgct
    11821 tctgccgcta atttttccaa gtaaagagtc tcgacgcctt cattttgcaa tgtcttcgca
    11881 aagttgtcat gttcttcttg agcaatcgga agatatggaa tatcatcgaa caacagacga
    11941 tccataatat ctggtgtcaa attttccact tcttgtccag gccgtttcag taaaactgtc
    12001 tttaatttcc ctatttcaga gaaaacgtga ataggtttat ccatgttgtg ttgcctcctt
    12061 ctttttgatt acatgtatag gatatcctcc ttttgtaatc actttcataa ttttgtctca
    12121 ttcaaaatga gacattcctc acattaatat tcgataatta aataattaaa cgtttttcta
    12181 tttttaagcg taatttattc attttttatt ctgtatacac tttcaaaaaa atatttattc
    12241 attgaaaaaa tttttttgaa taaattaaaa aaataaatta t
//
LOCUS       DARDHA010000082        12235 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000082 DARDHA010000000
VERSION     DARDHA010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.083"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(193..357)
                     /locus_tag="SDA10_001759"
     CDS             complement(193..357)
                     /locus_tag="SDA10_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240664.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            652..1980
                     /locus_tag="SDA10_001760"
     CDS             652..1980
                     /locus_tag="SDA10_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEG4240665.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2182..3015
                     /locus_tag="SDA10_001761"
     CDS             2182..3015
                     /locus_tag="SDA10_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEG4240666.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3031..3768
                     /locus_tag="SDA10_001762"
     CDS             3031..3768
                     /locus_tag="SDA10_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEG4240667.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3923..4891)
                     /locus_tag="SDA10_001763"
     CDS             complement(3923..4891)
                     /locus_tag="SDA10_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEG4240668.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5123..5956
                     /locus_tag="SDA10_001764"
     CDS             5123..5956
                     /locus_tag="SDA10_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEG4240669.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5990..6829
                     /locus_tag="SDA10_001765"
     CDS             5990..6829
                     /locus_tag="SDA10_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG4240670.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6855..7415
                     /locus_tag="SDA10_001766"
     CDS             6855..7415
                     /locus_tag="SDA10_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG4240671.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7614..9335
                     /locus_tag="SDA10_001767"
     CDS             7614..9335
                     /locus_tag="SDA10_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEG4240672.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9500..10642
                     /locus_tag="SDA10_001768"
     CDS             9500..10642
                     /locus_tag="SDA10_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG4240673.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10658..11869
                     /gene="thiI"
                     /locus_tag="SDA10_001769"
     CDS             10658..11869
                     /gene="thiI"
                     /locus_tag="SDA10_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEG4240674.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4165 a         2054 c         2544 g         3472 t
ORIGIN      
        1 tagtagcgca tgactgttaa tcatgatgtc gtaggttcga gtcctactgc cggagtaaat
       61 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
      121 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      181 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      241 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      301 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      361 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      421 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      481 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      541 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      601 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      661 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      721 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      781 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      841 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      901 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      961 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
     1021 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1081 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1141 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1201 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1261 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1321 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1381 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1441 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1501 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1561 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1621 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1681 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1741 gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1801 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1861 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1921 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1981 acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct
     2041 ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa
     2101 ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa
     2161 ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat
     2221 ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa
     2281 catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa
     2341 gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt
     2401 gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa
     2461 cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt
     2521 cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg
     2581 acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga
     2641 tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt
     2701 gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc
     2761 tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa
     2821 caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat
     2881 caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat
     2941 tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta
     3001 ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa
     3061 gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta
     3121 gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat
     3181 agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga
     3241 gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat
     3301 cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc
     3361 gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct
     3421 tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg
     3481 ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat
     3541 aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt gactacgcat
     3601 gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa
     3661 agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg
     3721 caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt
     3781 gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt
     3841 ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag
     3901 tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca
     3961 aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac
     4021 agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca taaacaggct
     4081 ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct
     4141 tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca
     4201 aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg
     4261 ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt gtttctaaaa
     4321 gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg
     4381 ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct
     4441 catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa
     4501 tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc
     4561 cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt
     4621 ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca
     4681 agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga
     4741 agatatcttc aactaataat tctacatgct tggggattgt gatttctgca tctaataatg
     4801 ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc
     4861 cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga ataattttgc
     4921 atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt gaagaataac
     4981 gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga ctcaaccttg
     5041 acctcctaat acaatggagt atctggttga gtttctttta caagaaagca gccaaggaaa
     5101 tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta aagaacatga
     5161 caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc tacccttagt
     5221 ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga attggataag
     5281 tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca gcggtcaagg
     5341 agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt tagatgttat
     5401 gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc cctcatcatt
     5461 caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt ttgagggtta
     5521 tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc ttgaacgtac
     5581 aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc cacagttgct
     5641 gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc tttttcgatt
     5701 ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta aaaaaagaac
     5761 gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca aagtagttga
     5821 ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg atgcggctga
     5881 aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca atccaaccca
     5941 aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta tgatcaaagc
     6001 tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt tggaatcaac
     6061 aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag caacggggcg
     6121 cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt atgtcgtgta
     6181 taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt ttgaacaaaa
     6241 agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg tttttggtgc
     6301 gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt ttatcaagcg
     6361 actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa tcctccaggt
     6421 ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa aagaaccaat
     6481 ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac atcgctttcc
     6541 cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa aaggtggttc
     6601 aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg aagaaattat
     6661 ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa ttggagtggc
     6721 catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc aatcaaatga
     6781 agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt caatgaaaga
     6841 aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca ttccacaaaa
     6901 cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa gctcatacac
     6961 gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca cctaataaag
     7021 aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca gaagaaaaaa
     7081 tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat gcactgacag
     7141 atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct gatcgaatat
     7201 tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa cctcattatg
     7261 accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat gctccagaac
     7321 ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag aagaaatccg
     7381 ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat ttttgttatc
     7441 tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg aaaatagtat
     7501 gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat ttatgccaag
     7561 cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca taaatgggaa
     7621 ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc gtctttttgg
     7681 ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa aaacgcaagg
     7741 aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa atgcatctgg
     7801 ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac atatctactt
     7861 cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat gaaagttttc
     7921 ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac caaatggaga
     7981 aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa gaacgcaact
     8041 cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa gtcagagaag
     8101 acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa gtgatcgaaa
     8161 aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa gcccgcgagg
     8221 ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa aaaattccac
     8281 cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc agtgatactt
     8341 acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct gaagatatcg
     8401 caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca gaagttgaaa
     8461 cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat gatatcttag
     8521 aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt gcagagtata
     8581 tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac acatctcaaa
     8641 gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc gaagtggaag
     8701 aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa attccttatt
     8761 cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc gaaaagcaac
     8821 aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa gcgcaagaaa
     8881 agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa aaacaacgtt
     8941 tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac cgtattgaag
     9001 aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat cgtttagttg
     9061 ctctatgtga agaagatcta gaattattag ataagaagac acatgattta gtagatgcag
     9121 cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca cacgaagaag
     9181 tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac tatcaagaag
     9241 cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc aaacgcatcg
     9301 aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt aaaccagcaa
     9361 gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa attgaaacaa
     9421 gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag aaaagaattt
     9481 caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc caatctttcc
     9541 gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc catcaagctt
     9601 gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc agatcgctga
     9661 tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag aaggagataa
     9721 ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata tgatcatttc
     9781 aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat taggaatcga
     9841 agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat tgaaagaatt
     9901 aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag taggatcgat
     9961 tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc atttccatgt
    10021 agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag aaagagttga
    10081 ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt tcatttattg
    10141 gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa gaaatcagcg
    10201 aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac gtttatcatt
    10261 agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc taatcaatgc
    10321 gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg caccgcatat
    10381 tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct tagaagaaaa
    10441 acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg caagcagtac
    10501 attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa tcagcttaga
    10561 tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc agctttataa
    10621 gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg aaattatggt
    10681 ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca tgcaactagc
    10741 acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg ctgatcggga
    10801 ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa aactagcaaa
    10861 aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg tcgaagtttt
    10921 gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa catttaaaat
    10981 tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga atcaaacatt
    11041 aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc aaccagatat
    11101 tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa cgatcaaggg
    11161 agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt ctggggggat
    11221 tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg aagctgttca
    11281 ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag atctaacagc
    11341 caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta ccgagattca
    11401 agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc gtcgaatgat
    11461 gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt tgaatggtga
    11521 atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca atgaagtaac
    11581 gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca ttgaagtagc
    11641 agaacagatc gatacattta atctttctat ccagcctttt gaagattgct gtacgatttt
    11701 tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat atgaagagcg
    11761 tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg aagagataca
    11821 agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag aggttatgaa
    11881 aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc atgtatttta
    11941 ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa gaagttatgg
    12001 aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat caagctacag
    12061 ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg acagaagtgg
    12121 gcagcttcaa gaaataagaa ggaatttacg aaaattgctt ttcaaatttt tgtgaaattt
    12181 cggcttattt ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt gcgaa
//
LOCUS       DARDHA010000083        11669 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000083 DARDHA010000000
VERSION     DARDHA010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.084"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(37..216)
                     /locus_tag="SDA10_001770"
     CDS             complement(37..216)
                     /locus_tag="SDA10_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240675.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(597..1304)
                     /locus_tag="SDA10_001771"
     CDS             complement(597..1304)
                     /locus_tag="SDA10_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240676.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="SDA10_001772"
     CDS             complement(1297..1734)
                     /locus_tag="SDA10_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240677.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="SDA10_001773"
     CDS             complement(1749..2000)
                     /locus_tag="SDA10_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240678.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="SDA10_001774"
     CDS             complement(2000..2206)
                     /locus_tag="SDA10_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEG4240679.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="SDA10_001775"
     CDS             complement(2643..2903)
                     /locus_tag="SDA10_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240680.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2978..3181
                     /locus_tag="SDA10_001776"
     CDS             2978..3181
                     /locus_tag="SDA10_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEG4240681.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="SDA10_001777"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="SDA10_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG4240682.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="SDA10_001778"
     CDS             complement(3810..4709)
                     /locus_tag="SDA10_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEG4240683.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="SDA10_001779"
     CDS             complement(4722..5315)
                     /locus_tag="SDA10_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEG4240684.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5391..5540)
                     /locus_tag="SDA10_001780"
     CDS             complement(5391..5540)
                     /locus_tag="SDA10_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240685.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5556..7760)
                     /locus_tag="SDA10_001781"
     CDS             complement(5556..7760)
                     /locus_tag="SDA10_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HEG4240686.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..9231)
                     /locus_tag="SDA10_001782"
     CDS             complement(7771..9231)
                     /locus_tag="SDA10_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEG4240687.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9292..11664)
                     /locus_tag="SDA10_001783"
     CDS             complement(9292..11664)
                     /locus_tag="SDA10_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240688.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2892 a         2433 c         1958 g         4386 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctggt agttttcgtt cttccttcag ataattcctt cctttttctg tgagtggttg
     8881 ataatcaacg gggtgatctt tgtacttttc tgtctcaaaa tgaagatttt ccactcgttc
     8941 tgttttgtct tcattcttta gaataaaatc agtggctgct ttaaattgtt gcttagggtc
     9001 ttggatctct ttgtctatga aaaaaagttt agctaattca ataatgtcgc cactaatatt
     9061 ttgactgttc caataaaaac gttgttttct tcgatcaaaa acaaatgaat catggtcttc
     9121 taagcgatag tctcgtcctt tattgactac agccattcca ttgtttcgag caaaatccac
     9181 tatatcgatc tgtttacatc gatcaataag actcattttt ttctcggtca ttttaaaacc
     9241 tcccaaaaga aaaaagacta tccgtttttc agatagtctt ttttacttgt tttatacacg
     9301 ttctttttgt gacaattgtt gttcataata gatttcattt tttcgttttg ccacttctga
     9361 cactgtctca agtgaaaatc ggaatgaatt tcctagcgca aattcttctg aaggtaacgc
     9421 cgtttggatc tcttttccat aactttttcc ttttaaaatt acttgattat gctgtggatc
     9481 ttcaataaac gccgcatcct tcaaatgtaa cgttcgaccc gtatcttctt ccattacttt
     9541 cccgtctttc ttgtcgatcc aaagtgaacc aacaggaaca atattctttt gttctgccgt
     9601 ttctacagta tttttatgct cctgcttcac ttctgctggt tggatctctg gttctctttc
     9661 tctgtattga ttgatttcat tagaaatggt ttgaatgaat tcatttgcga cacggctgac
     9721 atcattcatg attttcccac gttccgcagc ttccttatca tccaacgtct gaccattctt
     9781 cgtccatgct gcaatataag gaatagcttt ctccgaagtg tccattccaa aatgtttgca
     9841 cacaatataa gaagtcattt ctgcctgcaa ttctttagtt gcggtatcta attctgaaaa
     9901 tttgtgcatt cttgcatggg ctaattcatg aatacttgta gctaaatgct gcgtaggagt
     9961 atttcgagta ttcatgacga tttcttcttc gccagtaaat tcgttacgaa catatgcccc
    10021 ccgtgcttgt cctaattctc tttgatagac ttcactttct gtcatatctc gaatttgtat
    10081 tccaatattg ttggctaccg cttgaatccc agcttgaagt tctgactgtc cttttttatc
    10141 caattgaaaa tcaaagacac ggttcggaaa gattttcgga tagtcttcag gttttaactg
    10201 tgtttgtgtg atatcaaaca ctttttcaat cgtgtagtaa ggttttttga tcacttcgat
    10261 ttgaccactt tcgattcgtt ttttatcttc tggtgtagct tctgtttctc gaacaaagcc
    10321 acgtcctttc tcataaaatc ctttgaccgt ttttctgaca aagatcttgt tcccttgttc
    10381 cccttttttg atatagattc cttctttttt caacgcagca cgacctaaac aagccgttgc
    10441 acctggaaat tgttcctcaa tcaataattg atttctaaag gatcgttctg gaaaacgaga
    10501 cataaaattc aaaagttctt tcacgtcttc aggagaatgg gtatgctttt tcaccgcatc
    10561 cagtgcctgt tgcgaaaggg atttgacttc ttgcttaatc tcttctgccg ttcgtttttg
    10621 atagcccttt tgttttgttt ttgtttcttt cgactgcatt tgttctttcg aaacaggttc
    10681 atttttgacc gtttgttttt gttccttttc aggtacttgt tcgcacaatt ttgctaactc
    10741 taaatagatg ggttgattac tgttgacgcc atcccctaga tccattcgga tctgatcgac
    10801 tacttctccg ttcttcactt cttcaaaata gaatttataa taacctgctt ctgcggcgta
    10861 ttggttatcc aacgttttga gttgatcgat caattctaat gttacgatct ccccaccata
    10921 acttttcaat cctttttctg gatcattttc gttgaattct actcgataga aatgatcgtt
    10981 ttctttctcc ttcaaagaat cagttggcgc agcctcttca agtaattcac gaacagctgc
    11041 tttttcctct tccgacgtgg cttggtattt ttttgattcc tcaaatcccc cgttttcatt
    11101 cagttctaaa gcaacttttc gatagtcttt caaaaaataa atggttggat tttttttcca
    11161 ttgcgtcact cgtccgctgt caataattgc ttgtacttca ggaagaagat tttcttttcc
    11221 tttttctgat ctttctttca gctgttctaa tgtttccaat ttccgttggt attttcggat
    11281 cgtagcggct gaaaaagtcg attcaccttt ttcaaagcgt tcgatttctt ctttgattct
    11341 tggaatattt tcgattgtca tatccaggcc gccgtacttg ttatagccca tttcctttag
    11401 ctcttcttgt ctttcaagtt tttctacctg tttttcttgt tgcttgatct cttctctcag
    11461 actatgcact ttgttttcta aacgttcttg ttgcctaaac caagaatgcc catttctctt
    11521 atcgttcatt ggctgaccgt tggtctttct ctggtgttca aacatcttct caatttgttc
    11581 gtttaattca ttttgcaatc gtactaattt tcttttggat tttcccaagc gactattgtc
    11641 tagtggttct ttttcttctg ccatgtccg
//
LOCUS       DARDHA010000084        11490 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000084 DARDHA010000000
VERSION     DARDHA010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.085"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            8..649
                     /locus_tag="SDA10_001784"
     CDS             8..649
                     /locus_tag="SDA10_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240689.1"
                     /translation="MIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLSAV
                     LHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMRYQ
                     IDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIH
                     RFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="SDA10_001785"
     CDS             708..1148
                     /locus_tag="SDA10_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240690.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="SDA10_001786"
     CDS             1160..1828
                     /locus_tag="SDA10_001786"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEG4240691.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="SDA10_001787"
     CDS             1892..2515
                     /locus_tag="SDA10_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEG4240692.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="SDA10_001788"
     CDS             2571..2957
                     /locus_tag="SDA10_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEG4240693.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="SDA10_001789"
     CDS             3170..4561
                     /locus_tag="SDA10_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEG4240694.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            4908..5555
                     /locus_tag="SDA10_001790"
     CDS             4908..5555
                     /locus_tag="SDA10_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEG4240695.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    5684..5900
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5948..8593
                     /locus_tag="SDA10_001791"
     CDS             5948..8593
                     /locus_tag="SDA10_001791"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEG4240696.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            8903..10216
                     /locus_tag="SDA10_001792"
     CDS             8903..10216
                     /locus_tag="SDA10_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEG4240697.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            10206..10859
                     /locus_tag="SDA10_001793"
     CDS             10206..10859
                     /locus_tag="SDA10_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG4240698.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            10914..11465
                     /gene="radC"
                     /locus_tag="SDA10_001794"
                     /pseudo
     CDS             10914..11465
                     /gene="radC"
                     /locus_tag="SDA10_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
BASE COUNT         3795 a         1943 c         2480 g         3272 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctattgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     4741 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
     4801 caccgtttta tttgtctcac acccttttcc ttcctacttg taaccttgct cccgtgttag
     4861 aaacatgtta cactaaatat gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa
     4921 cagatacact gccaaaagca actgcgaaaa gattaccttt atatttacgc tacttgaaga
     4981 tgctggatga ctcaggtata agtagaatca aatcaaatga gttcagcgaa atcacccaaa
     5041 ttccttctgc aacgatccgc agagacttct cgcaattagg cgagctaggc agaagcggat
     5101 atggctatga tgtccctttt cttatcgatg tattcaataa tatccttaat actaaagaag
     5161 aaaaacgaat cgcattagta ggatatggaa atcttggcaa agcattgaaa cacaacaatt
     5221 ttagaagaaa tgaaaatcta aatatcgtgt gcgtctttga taatgatcca gcactgatca
     5281 atcgagtaat tgatggagaa atgatctatc cgattgatcg tttcgcggag atagccaaag
     5341 caaaaaatgt gacagtagca atctctacgg taccaagtaa atattcacaa tctgctattg
     5401 atgaaattgt caaaggaaat gtaaccgcta tacttaattt tgcgcctgac agagtaactg
     5461 tcccagctta tgtcaatgtt caatacattg atttgacaac cgagttgcag acgcttattt
     5521 actttgatga gaattactca gaagtttttt cttaggaaaa tttgagtagg cttattgatc
     5581 ttgcgagaac tcgtcgtaat tctttcaaaa attttttagt actaaatgaa gtggtttttt
     5641 cacgaccact tgttgactaa aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa
     5701 gaggagtaac tggcacattc attcttagag agagaactat ctgctgaaag gttctcaatg
     5761 acaaccagtg aaggtagctt gggagtttct gtcttgaatg gagtagagat agacgagtcg
     5821 tgatcgttaa ctcacttgag aggcaataat gattgattat tgcaatttag gtggtaccgc
     5881 gtaaatcata cgtcctagaa tacgaaagta ttttaggacg ttttttagtt taaaggagga
     5941 aaacagcatg acggaagaaa ataacctatc aacaaaatat aatccgcaag aagttgaagc
     6001 tggacgttat cagcattggt tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc
     6061 aaaaccttat tcaatcgtaa tcccgccccc aaatgttact ggaaaacttc atttagggca
     6121 tgcttgggat acgaccttgc aagatatgct gattcgtcaa aagagaatgc aaggatatga
     6181 tactttatgg ctgcctggta tggaccatgc aggaatcgcg acacaagcaa aagtagaagc
     6241 gaagcttaga gagcaaggga tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca
     6301 agtgtgggaa tggaaagaag agtatgcgag ccacatccgc gaacagtggg ctaaaatggg
     6361 cttatcttta gattacagta gggaacgttt tacattagat gatggattat ctgaagcagt
     6421 acgtaaagtt tttgttacct tatacgaaaa aggtttgatt tatcgtggtg aatatatcat
     6481 caactgggat cctcaagcac gaacagctct atctgatatc gaagttattc ataaagatat
     6541 cgaaggcgcc ttttatcata tgagctatga actagcagat ggatcaggcg tagtagaaat
     6601 tgcgacaacc cgtccagaaa cgatgttggg agatacggcg attgctgttc atccagaaga
     6661 cgagcgttat caagcgctca tcggcaaaaa agtgattcta cctttagtca ataaacaaat
     6721 cccaatcatt gcagatgaat atgtagatat ggaattcggg acaggtgtag taaaaatcac
     6781 tccagcacat gatccaaacg actttgaggt aggtaatcgt catgatcttc cacgcgtaaa
     6841 tgttatggat gatgatggta cgatgaatga actagcagga aaatatgctg gaatggaacg
     6901 ttttgctgct cgtaaagcaa tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat
     6961 cgatacgatg atccacagtg tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg
     7021 gctgtctact caatggtttg taaaaatggc gccgttagcg aaaaaagcca ttgataacca
     7081 agaaacagat caagcagtgg aattcttccc tccaagattc aatcaaacgt ttttacgttg
     7141 gatggaaaat gtccatgatt gggtaatctc acgacaatta tggtggggac atcaaattcc
     7201 tgcatggtat cacaaagaaa caggtgaaat gtatgtaggg atggaagcac cagcggatag
     7261 tgaaaactgg gtacaagatc ctgatgtttt agacacttgg tttagttcag ctttgtggcc
     7321 tttctcaaca atgggctggc cagatacgga aagcgaagat tataagcgat acttccctac
     7381 tagcacttta gtaaccggtt atgacatcat tttcttctgg gtaagcagaa tgattttcca
     7441 aagcttggaa tttactggcg aacgtccatt tgagaatgtc ttgattcatg gattgattcg
     7501 tgacgaacaa gggcggaaga tgagtaaatc attaggtaat ggaatcgatc cgatggaagt
     7561 aatcgaaaag tatggagcag atgctctccg ctggttctta tcgaatggtt ctgcaccagg
     7621 acaagatgtc cgcttcagct atgaaaaaat ggatgcttct tggaacttta tcaacaaaat
     7681 ctggaatgct tcacgtttcg ttatcatgaa tatcgaagga atgaccgtag aagagatcga
     7741 tttatctgga gaaaaatcag tggctgaccg gtggatcctt actcgtttga atgaaacgat
     7801 cgaacgagta actgagctat ttgatcgttt tgaatttggt gaagcaggtc gacaactgta
     7861 taactttatc tgggatgatt tttgtgactg gtacatcgaa atgagtaaag aagtacttta
     7921 tggagatgat gctgtttcaa aacaaaccac ccgtagcatc ttagtccata cacttgatca
     7981 gatcctacgt ttattgcatc caatcatgcc attcgttaca gaagaaatat gggaacatat
     8041 tcctcatcag ggaaattctc tagtagtggc agaatatcct gttgtacgtc cagagtttga
     8101 tgatgaaaca gcttctaaag ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa
     8161 tattcgttca gaagtgaata caccattgtc taaaccaatt acgttgctaa ttaaaacaaa
     8221 cgatgctaaa atcgatcaat ttttagttga aaatacaagc tatatcgaac gcttctgtaa
     8281 tcccgaagag ttgacgatat caagtgagat cactgcgcca gatctagcga tgtcagctgt
     8341 tctgacaggt gcagaaatct tcttgccgtt agcaggattg atcaatattg aagaagaaat
     8401 caaacgatta gaaaaagaac tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa
     8461 actttctaat gaacgttttg tttctaacgc gccggaagaa gtagtagagg cagagcgtgc
     8521 aaaagaaaaa gattatttag agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg
     8581 tactattcaa taagaataga aaaagagagt gtgaaatcag ctagctatta ctaaaagcta
     8641 gctgattgaa catcgttaca ggaaagagtt tgagcctcac tcttagcttc gattcataag
     8701 gagatgtgac ataaattttg tcgcatctct tttttagtct actcttatta tgtaggagaa
     8761 ggagggaggt ggatcatgta tcaagagtca ataaagtagt tgataaaatc gttcctctct
     8821 ctgtttcttt tctctttgtc atacttttat ttttcttatg tgttctattt gttataataa
     8881 aagagaaatg ggggaattgt tgatgacgat tgaagaagca atagaatgga tccatagtcg
     8941 attaccattt ggttcacgtc ctggccttga ccgagtcgaa gctttattag aagtggtagg
     9001 gcatccagag agaaaagtaa aaacgatcca tatcgctgga acaaacggaa aaggttctac
     9061 tgtagcttat ttgagaagcc tattagaaga ggctgggctg acagtaggta catttacttc
     9121 accttatatc gaaacgttca atgaacggat cgcaatcaat ggcaaaagca ttccagacaa
     9181 tgatttgatc cagcttgtaa aaaaatatca gccactgatc aaacaacttg atcaatttga
     9241 agcagtagct ggtattactg aattcgagac attaacagca atggcatttg attatttttt
     9301 gatgaagaaa gtggacgtag cgattatcga agttggctta ggcggcttat tggattctac
     9361 caatgtagct gtaccaatgc tgacgggaat aacaacgatt ggacttgatc ataccgaaat
     9421 acttggagat acaattgaag aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt
     9481 tccagtagta acaggaaata ttgccccaga agcttttact gtcatccaac aaacagctaa
     9541 agcaaaaaat gcaccgcttt atgtatacgg ggaaaattat aaaataaact atctgcatcc
     9601 tgatgagatg tggggagaag tatttgaatt ttttaatgaa ataggaaaga tccctcaatt
     9661 aaccactccg cttttagggc aacatcaagc tgaaaatgca ggagtagcca tcgaattgta
     9721 ttttgtttac tgtcaattgg aaaagctgcc tttccgagaa aaagatgttt tgaagggatt
     9781 gaagaaatct cgatggccag gaagaatgga gcgtttgagt aacgagccat tagttgtttt
     9841 agatggtgcc cataatacgc atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt
     9901 caaaggatat caaatcaaca ttttgttttc cgccttacga actaaaaatg ttagagaaat
     9961 gctagaacga ttgcagcaag taccaaatgt ccatatttat ttgacgacct ttgaacatcc
    10021 aaaagccatc gaattaagtg aagtatcaga tttagcaagt gagagagtat caatcgtttc
    10081 attgtggcaa ttcggattag cagatatatt ggaaaagatg acaaatgaag acgtattagt
    10141 tgtgaccggt tcactttatt tcatttcaga agttcgccat ttattaatac aattaggagg
    10201 agatcatgaa gtataatgca gtgatttttg acatggatgg tttgctgttc gacacagagt
    10261 tagtgtatta cgaagccaca caagtagtag cagacaagat gggttttcct tatgataaag
    10321 acctgtattt aaaattctta ggtgtttcag atgaagaagt atgggcgaat tatcatatga
    10381 ttttctcgga atttggcaaa acaagtgtac aaagattcat agatgattca taccaggaaa
    10441 cgcttgaacg tttttcgcaa ggagcggtac aaatgaagcc cggtgtgttg gaactcctct
    10501 cttttttaga agaacatcag attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca
    10561 ttgaactttt attgaagaag aacaatctgt ttgaacgatt tgagacgatt gtttctgcag
    10621 aaaatgttca acgagcaaaa ccagacccag aaattttctt gttagctaat cagaagctag
    10681 gtacagcgaa gaaagaaaca ttgattttag aagattcaca aaatggtatt ttagcagctt
    10741 ctgcggctga gatcccggta gtcatggtac cagatctgct tccaccatcc caagaattga
    10801 tcaaaaaaac agctggggtt gtttcttctt tacatgaaat ccctgcattg ttaaaataaa
    10861 aatccatctc ttttcgtatc ttattttaga taagatagaa aggagatgtt tttttgggaa
    10921 aacgattgat caaagaagta cctactagct ctttaccaag ggaacgtatg gaaatttatg
    10981 gcgcggaagc tttatcggat caggagcttt tggcaatctt actgcgtacc ggtcaacacc
    11041 cttatagtgt gatgtcgatt gctggaaatc tgttaaaaac gtttggagga ctggcctctt
    11101 tacgacaggc tactttgcat gagttagaag agattcgagg gataggacgc gtgaaagcaa
    11161 tcgaaatcaa agccttgatc gaattaggtc gacgaattca gacggatcat gaaagagcgg
    11221 ctccaccggt tcgtaccagc tacgaattgg cccagcaatt gattctggaa atgaaagatc
    11281 acaaacaaga acatttggtt tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa
    11341 aaacagtgtt catcggttcg ctgaatcagt cagtcgctca tcctagagaa attttccatt
    11401 atgctgtacg ttattgtgca gcaaggatcg ttttagcgca taatcatcct tctggtaatg
    11461 ttttaaccat agtataataa ataatagtag
//
LOCUS       DARDHA010000085        11198 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000085 DARDHA010000000
VERSION     DARDHA010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.086"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            160..1419
                     /gene="lysA"
                     /locus_tag="SDA10_001795"
     CDS             160..1419
                     /gene="lysA"
                     /locus_tag="SDA10_001795"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEG4240699.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1406..2206
                     /gene="dapF"
                     /locus_tag="SDA10_001796"
     CDS             1406..2206
                     /gene="dapF"
                     /locus_tag="SDA10_001796"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEG4240700.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2209..3090
                     /locus_tag="SDA10_001797"
     CDS             2209..3090
                     /locus_tag="SDA10_001797"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEG4240701.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3087..3803
                     /locus_tag="SDA10_001798"
     CDS             3087..3803
                     /locus_tag="SDA10_001798"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEG4240702.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3809..4504
                     /gene="dapD"
                     /locus_tag="SDA10_001799"
     CDS             3809..4504
                     /gene="dapD"
                     /locus_tag="SDA10_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEG4240703.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4520..5521
                     /locus_tag="SDA10_001800"
     CDS             4520..5521
                     /locus_tag="SDA10_001800"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEG4240704.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5518..6879
                     /locus_tag="SDA10_001801"
     CDS             5518..6879
                     /locus_tag="SDA10_001801"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEG4240705.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6839..7990
                     /locus_tag="SDA10_001802"
     CDS             6839..7990
                     /locus_tag="SDA10_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEG4240706.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7965..9134
                     /locus_tag="SDA10_001803"
     CDS             7965..9134
                     /locus_tag="SDA10_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG4240707.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9309..10688
                     /locus_tag="SDA10_001804"
     CDS             9309..10688
                     /locus_tag="SDA10_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG4240708.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10969..11198
                     /locus_tag="SDA10_001805"
                     /pseudo
     CDS             10969..11198
                     /locus_tag="SDA10_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3729 a         1953 c         2499 g         3017 t
ORIGIN      
        1 tgtttttata accattttta ttcttatttt taaaaaaata tttcgaaata tactagacaa
       61 ttcagaaaat tttgtttaat attattccaa taaaattcaa atctttatca aatgaaattc
      121 caataaaaac ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt gataattggc
      181 ggagtcacag caagcacatt ggcagaaaag tataagacac ctctgtatgt gtatgatgaa
      241 gaaaagatga gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa acgttttgat
      301 acaaaagtgc tttacgcctc taaagcattc caaacgattg agatgctgca tttagttcgt
      361 gaatatggat tcgggctaga tgttgtcagt ggtggggaaa tctataccgc tttgcaagct
      421 gattttccta gagatactat ctacttccat ggtaacaaca agacgccaga ggaactgatc
      481 tatgcacttg aaaatgattt gttgcatatt gttgccgata atttgatgga agttgaatta
      541 ttagcagaat taagccaacg ctatttaaaa aaaatctgta tcatgcttcg gttgaatgtc
      601 ggagtagaag cacatactca tgaatatatc gtcactgcgc atattgattc aaaattcggc
      661 atgagctatg aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga aagcagctat
      721 ctagagttag aaggatttca tgctcatatc gggtcacaaa tttttgatgt tactgcttgg
      781 ttagctgaaa tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc tctttccttg
      841 aatcttggcg gagggtttgg gattcgttat acacaaaatg atcagccgat cccagtcgaa
      901 gaggcactaa aaaaactgat tgaatatacg gaagaagctt tggaaaaacg aaaactgacc
      961 attcgtcaat tactgatcga accgggtaga agtattgttg gagaagctgg tacaacacta
     1021 tacacgattg gtttcatcaa aaagacacca cataaaaaat attactttgt agatggcggg
     1081 atgacagata atatccgtcc agctctgtat caggcagaat acgattgtga tttagcagca
     1141 aaactgtcgg aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc
     1201 gatgtggtaa ttaaagaagc ttatttgccg aaagcagaat cgggtgatct tttagctgtt
     1261 tattccacgg gagcttatgg atactcgatg agcagcaact acaatcgagc gacacgacca
     1321 gcagttgttt ttgtcaataa tggccaatca agactgattg ttcgcagaca agcattttct
     1381 gatctactaa ggggggaagt ttcctatgat ccttcataaa tataatggct gcggaaatga
     1441 ttttatctta ttagattacg cagaagacac agattatagt caattagccg cttctttatg
     1501 cacaaaagaa aaatatgata cagacggatt gatcgccgta aaaatcgatc cgttggaaat
     1561 gatctattac aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt
     1621 tgctcgatac gtaaatgatc agaacatgat gaatgaacag gaattcaacg tagagacatt
     1681 agcaggcaca agaaaaatta cggtgttgga tccctcacct ttttattgca gcgtagatat
     1741 gggagtgcca gatgattctc cggaaaaaat caaagctaat caaacttatc caataaaaaa
     1801 ccaggaaata gagatcaatg gtgaaaaagt aacgatcact agtttatttt taggaacgat
     1861 tcatacagtt gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact
     1921 gatctgtcat catcctttgt tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa
     1981 tgaacaagaa ttgatcgtcc gtacctatga aagaggagta ggttggacac ttgcttgcgg
     2041 tacaggttgc tgtgcctctt atgtggttgc taaggaaact gggaaaattt cagcagatca
     2101 ggtagttgtt cacttggaac aaggagactt gatgatctct ggtgaggatg cgatacaaat
     2161 ggctggccca gcagttcatg aatggacaag aatattggag gaatgaagat gataaaaggt
     2221 tcgatcgtgg cgctgatcac gccgatgaat gaagagggat cagtagatta tgctggtttg
     2281 gaaaaattga tccaattcca tttagatgaa caaacagatg gattattagt tttaggtaca
     2341 acaggagaat cttctacgct cacacaatct gaggaggagc agatccttca actgacagta
     2401 aagaaagtgg ctggccgtgt gccggtgatt gcaggagctg gaacgaataa tacgaaagaa
     2461 acgattgaaa aagcgaagca ttttgcttct ttgggggcag acgcactttt ggtcatcacc
     2521 ccttactaca acaaaacaag tgatgcagga ctagctgctc attttacagc aattgcagaa
     2581 gcctcaccga ttccgcttat tttgtataat gttccttcta gaacaggcat gtcgattcca
     2641 atccatgtat tagtaaattt agcagaacat cctaatatca ttggtttgaa agaagcctca
     2701 ggagatatgg cttatgtgat ggatgccgct cggttgattg gagaagaatt cttcctttac
     2761 agtggaaatg atgatttgat cctaccagta atgagtgtag gaggaagcgg tgtaataagt
     2821 gtatgggcga atatccaacc taaaatagtc catgaattag taaaagatac gcaggacgga
     2881 agatggcagc aagcaaaaga aaagcaatta aatgcacttg aactgatcca tgccttgttc
     2941 agtgagacca atcctattcc tgtcaaagca gccatgtcat tattagactt accttccgga
     3001 cctctacgcc tgcctttagt ttctctttca aaagagaaaa agaaacaatt agctcagctt
     3061 ttgttaaaag aaaggacggt tgaaaaatga aaatcggact gattggcagt ggacagatgg
     3121 gaaatagagt cgaagaagtt gcacaagaaa gaggagatac ggtccttctt ttacggacgg
     3181 cacctaaaga agcaactacg cttgctttta cggaactgcc agaactgctt attgattttt
     3241 ctcatccaga taatttagag aaaatccttt cgtatagctt atcatatcaa cttccgttag
     3301 tcattggcac gacaggttat acacaatcac agttccaaga aataaaagaa catgccaaac
     3361 atgtacctgt gatgtatagt gctaattttt cgttcggcat catggtgatg aatcaactaa
     3421 tcaagcaagc agctgcaatg ttgcaaggct ggcagatcga actgattgaa aaacatcata
     3481 gtcgtaaaaa agatgcacca tctggtactg caaatatgct gcttcaaaca atccaagaag
     3541 tgcaaaagct gcagcccgta tacaactggc aagggaaaaa aagagaagaa aaccaaattg
     3601 gtgtacacag tattcgtgca ggttctttac ctggtgaaca tgatgtattg tttgctgcta
     3661 ctgatgaaat atttacgatc aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg
     3721 cagtagaagc agcggactgg ttgaaagatc agtcaccagg ttattacaaa ttagaagatt
     3781 tattaatgga caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc gctacattca
     3841 aacggcatct aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt
     3901 taaagaaagt aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg actatgagac
     3961 gattgctccg gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc
     4021 tgcaagatac agcgcagttc ctttgctaga tattaaaaaa attaatgctc gtatcgaacc
     4081 aggtgctatt atcagagatc aagtgtcaat cggcaacaat gccgtgatca tgatgggagc
     4141 gattatcaat attggtgcag taatcggtga aaacacgatg attgatatgg gtgctgtctt
     4201 aggtggacga gcgacagtgg gcaaaaactg ccatattggt gcaggagctg tcttagcagg
     4261 tgtgatcgaa ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc
     4321 gaatgcagtg attgttgaag gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc
     4381 agtggtttta gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt
     4441 aaaaataagt gatgatcaga caaaagaaaa tacagcattg attgctgcgt tacgcgaatt
     4501 atagaggaga tggagaaaaa tgcgcgaaat agatacctgt attgtggggg caaccggatt
     4561 agtcggccaa acgatgctca aagtgttaga agaatatgat tttccagtag gacgattgaa
     4621 attattagct tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa agaaacacac
     4681 tgtacaggaa ttgacagaaa cttcatttga aggatatgat ttagcgctat tttctgcagg
     4741 tgcttctgta tccaaagagt ttgcgccaat cgcacgaagt cagggactat ttgtcataga
     4801 taattcctca gcatggcggg aagattcgga tattccatta atcgttccag aggtcaatct
     4861 ggaagactat acattgaatc ggctgattgc aaatccaaat tgttcaacca ttcaagcggt
     4921 acttccgctt aaagctttac aagaagcctt tggtatccag cgagtcaatt attcgaccta
     4981 tcaagcagtt tctggttccg ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga
     5041 cgaggaacca gttttgtatc cttataatat tagccaaacg gtgattccgg aaatcgatca
     5101 gcctttggaa aatgggtata caaaagaaga acagaaaatg ataaatgaga cccgtaagat
     5161 tctgcatcag cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg
     5221 acatggagtc tctgtggcag ttcaactgca aaaagagttt acgacggaag aaattagaga
     5281 gtgtctcagc cagtttccag ggattcaatt agaagatgat ctagcagcac ataaatatcc
     5341 gacgagcatt cttgctagag gaacagatat ggtttatgtt gggaggatca gaaaagacac
     5401 ttctttagag aacggattgt tgttctatac gactgccgat aacatacgca aaggagccgc
     5461 tgcgaatgcc gtacagattg ctgcagcttt aatgcagaaa atagcggagg tggcaagatg
     5521 agtaatacgc tgaaagtagc gaaattcggc ggaacatcga tgtctcatga agagcaattc
     5581 aaaaaagtcc gtacaattgt tctttcagat cccaatcgcc aagtgatcgt tgtttctgct
     5641 ttaggcaaac gtacgtcaga tgatgacaaa gtaacagact tactttattt gatttatgct
     5701 taccttcagc atcatgttga ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt
     5761 caagtaagag atgttcttgc cctgcagtta cctattgaag atgaactagc acgcatacag
     5821 aaacaactgg aagacgaaaa gataactgaa gattatttag taagtagagg tgaatatctg
     5881 actgctcaac tgatggctga gtatcttggt taccagttta ttgacgcaaa agacctgatt
     5941 ttctttgact atgatgggaa aatcgatcag gaaaaaacag cacaggaaat cacaaaagca
     6001 tttaaaagat atccgaaaat ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa
     6061 gaaaaattat tgggccgtgg gggaagcgac attaccggcg cgatactggc gaagatgttg
     6121 caagcggaaa gttatgagaa ttggacggat gtttcaggaa tcatgatggc tgatccgcga
     6181 atcatcgatc atcctaaaat catcgaggaa atctctttta gagaactgag agagatggct
     6241 tatatggggg ctaacgttct tcatgaagca gctgttttcc cagttcaaga ggctgatatc
     6301 cccatccaaa taaaaaatac caatgatcct ttcgctcaag ggacaaaaat atcgaatcac
     6361 gaaatagaaa aggaaaatgg actgacagga atagctggac gcaaggattt tctttccatc
     6421 actttattca aacgtcatat gtccgatgaa atcgggttta tctggaaagc aatgagcatt
     6481 tttgctaaac atgagatatc aatcgaacat attccttcag ggatcgacaa tatcggagtg
     6541 atcgtctcag cagaagcgat tgccgataag ttatttctca tcacgaaaga attaaaagaa
     6601 acacttggcg tggaagaaat cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg
     6661 ggtccgcata aagaattgat tggattatct gggaaagtgt tatcgatatt gaacaaatta
     6721 gaaattcgca catcgatctt atcacaagga gcacaggagt tgaatctgat catcggtgta
     6781 ccgaataatc aatatgaaac agtagtgaag ggtatctatg aagggatggt gaatacggat
     6841 gctgaaagaa tatcgaatgg cactccatca aattcctgaa atcgggtttc atgaattcaa
     6901 gacaaaaaaa tatttatatg aacaagtaaa aaacagcggg gccgttatcc atgaaatcgg
     6961 cgaaacagga ctactccttt attttgataa tcagcaagaa gaaacgattg cttttcgaac
     7021 agatatcgat gcactgccag tcaaagaaga aacgaatctc tcttttgctt ctaagcatga
     7081 aggttatatg catgcatgtg gtcatgacgg acatatggcg atgcttttag ggttgactga
     7141 ctttataact aaacacattg aggatcttat ttataatgta gtactgatct atcagccatc
     7201 agaagaaatt tccggtggcg cacaaagcgt tattcagtca ggattgttgg ataaatatga
     7261 agtacaagca atatttggct ttcatctatg gcctggacta gctaagggag aagttttctc
     7321 tcgaccagga cctttgatgg cacaaagcag tgaaacagac attatcatta aaggccagtc
     7381 tgcacatatt gcttctagtg aaaaaggaat cgatagtctg gaggcagctg tacgattttt
     7441 gaagaatgtt tatgattttg accggcaatt accggcagag tggaatcatc taatgaaatt
     7501 tggccaaatc caaggtggaa cgatccgcaa tgttttagct ggagaagtta ttgtatccgg
     7561 aagtatccgt tcttacagtt cagatatgca aaattatttg aagaggggat tagaccattt
     7621 agcagaacag tttcactcta cagctactat ttcttttcgc tataatgacg gctatccagc
     7681 tgtaatcaac catagaggct tatatgcagc actgttagat tcaaacaaac tacatgaatt
     7741 agatgctcca gttcttcaag ccgaagattt tggcatatat acgcaacaat atccatgtgt
     7801 atttttcttt ttaggtgtcg gtgatacccc tgctttacat gatccgacgt tcgattttga
     7861 tatggatgtt ttagaaaatg gtgtagaatg gtacaaaacg attcttttta cagaaaatct
     7921 tcttgtaaaa atcgatagca gttcaaaaga ggagcgagta aataatgatg aatttatcca
     7981 atcgatttag tcctaggctt tctagaatag aagtatcaaa aatacgacaa ttcgatcagc
     8041 aaatatcgtc gataccagat gtgatcaaac tcaccctggg agaaccggat tttccaactc
     8101 ctgaacatgt caagcaagca ggaatagcag cgattgaaga agacttttct cactataccg
     8161 gaatgagagg attagaggaa ctgcgggaag cggcttgtat atttcagcaa cagcgttatg
     8221 gattaactta tgatccgcaa acggaagttt tgacgacagt tggtgcaacg gaagcgattg
     8281 cctctgcgtt gttatctgtt ttggaagaag gagacaaggt tcttattcca gcgcctgctt
     8341 attcaggata tcagccatta gtggaactag ctggtgctga attgatcccg atagatactt
     8401 ctgacacagg gtttgtctgt cagccggaac aatttgaacg agcatttgaa caatatggat
     8461 cagctgtcaa agcagtcatt ttaaactatc ctaacaatcc tactgggacg acattgtctg
     8521 caaaacaatt gaaagcaatc gctgaggttt taaaaaaata tccggtattt gttataagtg
     8581 atgaagtata tgctgaattg acttatagtg gaacgcatat gtccatagct acttatcttc
     8641 cagaacaaac aatcgtgata agcgggttat ccaagtcaca tgcgatgact ggttggcgag
     8701 tgggctttat ttttgctcaa aaagccctta ttgacgaact aattaaagtc catcaatatc
     8761 tggtgactgc cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg
     8821 tgaaagatag cgagaatatg aaagagcaat atcaaacacg aagagactat ctggtccagc
     8881 aattggcgcc tttaggattt gaagtgaccc agccaaacgg tgcattttac ttgttttgca
     8941 aactcccagc tgccattcaa actgactctt ggcagttttg tgtagaatta gcggaacagg
     9001 caaaagttgc gtgtatccca ggaattgcat ttggcccaga aggagaaggt tatatccgga
     9061 tttcttatgc tagcagcatg gaaaaattac atgaggcctg caaacggatc aaagactttt
     9121 taacagaaca gtgattcata gattttatca aaaaaacaat cgttaatttt gcggtatatt
     9181 ctgcaaggag acgattgttt tttaaattta gttcaaatga acgcgtttct tttgattcat
     9241 aactaatgta atgttaagtt taaattgttt agaatgattt taaaagaata ttcaaggagg
     9301 tttcatcaat ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga gggcatcaat
     9361 cgatcttgga actattgaca cgatacggtg aaaatgtaga tatcacgcta tttgaagcag
     9421 gagactatat ttccttcatg tcatgtggta tggagctgta tttagaagac aaggtaacag
     9481 atgtaaatga tgtacgtaat ttcagaccag aaaactttcc gcaatccaat gttcatattt
     9541 taaataatca cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg gttactagcg
     9601 catcagataa cgaaacaaaa gaatatcctt atgacaaatt gattttgagt tctggtgtca
     9661 aaccaaacaa tctgccagta ccaggagcag atctagaaaa tgtctacttg atgcgtggat
     9721 acaattgggc aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg
     9781 tcgtaggtgc gggttacatt gggatcgaag cagcagaagc aagccgaaaa gctgggaaag
     9841 aagtcgtatt gcttgatgtg atcgatagac ctttaggcac ttatttagat gctgagatga
     9901 cagatatttt agaacaacac ctaaatgaaa acggtattga agttttgaca aatgcaaaaa
     9961 tcgaagcttt tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc
    10021 cagcagatct agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga
    10081 tcgtcgactt agatgaacgt ggctggatcc aaacaaatga atacttgcaa acgaatcttc
    10141 cagatgtgta tgcagtagga gatgctacac ttgcttattc tatcccagca cgcaaaaaaa
    10201 tgccgatcgc tttagcgact gttgcacgta gagaagcacg ttatgtagta aaacatcttt
    10261 ttgaagaagt accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct
    10321 ttgactatca ttttgcagca agcggattga atagctttac agcagcaaaa gcaggagtag
    10381 atgtacgagt gtcttattat gaagatacgt tacgaccaaa atatgtacca gaagaacaag
    10441 gaaatccaaa agtgtccatc caattattct cggatccaat gacccatcaa ttgctaggag
    10501 gagcagtatt atctacctat gatgttacag gtcaaggaaa tgtattggca ctagcgatcc
    10561 aaaacaaact gactttagaa gaactagcag aagctgactt cttcttccag ccaggctttg
    10621 accgacaatg gagcatgttg aatctagccg ctcaacaagc gttaggagaa gaaccatttg
    10681 tagaataaga aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta
    10741 ataagaacag ccaagtaaaa acagcttctt atgtttttaa cttggctgga cttattaccg
    10801 cagatgcaag cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca cgatttgtgt
    10861 accgaccccc aaaagttaga ccaaaaaatc taacttttgg aggtcggtat tttttatggt
    10921 taaatatact tttgaactta aattaaagat cgttcatgat tatttggatg gcaaaggcga
    10981 ttggaccgta aaaatccaga tcaagagatt gaagcggaaa tgcttaaaat tcgggagaag
    11041 cacaaggatt atggctgttt acggatgacc aatgaactaa gaaaccgcgg tttctctatc
    11101 aataaaaaga aagttcagcg cttgatcaag aagctaggaa taaaagtaac ggcatacacg
    11161 cgtaaatcac gtcgctacaa ttcttatcga ggaaaagt
//
LOCUS       DARDHA010000086        11071 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000086 DARDHA010000000
VERSION     DARDHA010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.087"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            361..1800
                     /locus_tag="SDA10_001806"
     CDS             361..1800
                     /locus_tag="SDA10_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4240709.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2095..2313
                     /locus_tag="SDA10_001807"
                     /pseudo
     CDS             2095..2313
                     /locus_tag="SDA10_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2562..2777
                     /locus_tag="SDA10_001808"
     CDS             2562..2777
                     /locus_tag="SDA10_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240710.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(2994..3250)
                     /locus_tag="SDA10_001809"
                     /pseudo
     CDS             complement(2994..3250)
                     /locus_tag="SDA10_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3437..4348)
                     /locus_tag="SDA10_001810"
     CDS             complement(3437..4348)
                     /locus_tag="SDA10_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG4240711.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4367..5371)
                     /locus_tag="SDA10_001811"
     CDS             complement(4367..5371)
                     /locus_tag="SDA10_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEG4240712.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5368..7491)
                     /locus_tag="SDA10_001812"
     CDS             complement(5368..7491)
                     /locus_tag="SDA10_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG4240713.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7496..9943)
                     /locus_tag="SDA10_001813"
     CDS             complement(7496..9943)
                     /locus_tag="SDA10_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG4240714.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(9930..10319)
                     /locus_tag="SDA10_001814"
     CDS             complement(9930..10319)
                     /locus_tag="SDA10_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG4240715.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(10379..10882)
                     /locus_tag="SDA10_001815"
     CDS             complement(10379..10882)
                     /locus_tag="SDA10_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG4240716.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(10895..11056)
                     /locus_tag="SDA10_001816"
     CDS             complement(10895..11056)
                     /locus_tag="SDA10_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240717.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
BASE COUNT         3228 a         2328 c         1993 g         3522 t
ORIGIN      
        1 atatcatagc aatgtatttg aaaagcaaat tggaaaacaa ataggtgcaa caccataaat
       61 atgaaaaaat tgggaaaaag tagaatggaa atttgcttac gtctcacgag atagctcaca
      121 ctattaatct gaagaacaat gaagtcgact tttagaaatt gctagaagaa agtaaagcga
      181 attatcacta gttactatag agtaagttca aataaagcat ctccaactta catggttaca
      241 tgaggatatc aaatcgaaat tactcaagct tcctatggct agagtcgcga gaagttgttg
      301 ggcagtagac ataggttata ttgttcttag ctacacgatc attagaaagg aaatcctatt
      361 atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc gctggtggat tttgattgct
      421 atatctatgt ttaccttcat gtccacattg gatggcagta ttgtcaatat tgcactacct
      481 accatctcag atgatatggg cataccaatg aatcaagcgg aatgggtagt gtccgtttat
      541 ctgatgattg tctgttcttg tttgttgctg tttggtaaaa ttggcgatag ccgtgggaaa
      601 atcaaagtat ttcggattgg tacgctgatc tttgtcttgg gatcgctgtt gtgtgggttc
      661 aatcattcgc taggtatttt attgttcgca cgggtggtac aagccattgg tgctagtatg
      721 actatggcta caaatgccgg aatcatcacg gaagtctttc ccatacatga acgtggtcgg
      781 gcattgggag caataggtgc gtttgtttca ttaggctcga ttgctggacc aggtcttggc
      841 ggcttgattt tgtcccaatt ttcttgggcg tacatttttt ggatcaatgt accggtggga
      901 atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag atatcaccat gagcggccat
      961 acaatcgatg gagggggctt tgctgccatt gcggctacga ttatcagctt ttttggagca
     1021 atctttttgg ggcaagaaag tggctttttc catccagtcc cccttgtact gttcgtaatt
     1081 tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga aagcagcctc tccattgatt
     1141 tctttcggta tttttaagaa caagattttt accatgagct tgattacagc cttgttgatt
     1201 ttttcttcca acttctttgt caatgtagtg attccttttt atctgcaaaa tgctcgcggg
     1261 ttgagtccta gctatgctgg gctgttgatg atggtctttc ctttgatgat ggtcgtcggg
     1321 gcgcctttaa gcggctacct gaccgaccgg gttggcccgg agattttggt attagtaggc
     1381 ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt tgtcagtgga ctctcctttg
     1441 tggctataca ttgtcgctac tgccatcatg ggtgccggca atgccttgtt ccagtccccc
     1501 aataatacga tggttatgag ctcggtcact atcgaaaact tgggactggc aggctcgatg
     1561 aattcctttg cccgaaattt ggggatggtc agcgggattt cgctttctac gacggtctta
     1621 tatcaagcga tgtctgctaa gatgggccag caagtaacta actatatcgc aaatcggccg
     1681 gatgtctttt tatatggaat gaagattacc tttttaggca gctttgtttt gtgttttgtt
     1741 gcctttttcc tgactatctg gcgtatcaaa aaacaaggag gtattcagaa aaatgtctga
     1801 aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg gtgagtggga tttccatgga
     1861 tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc aagtattatc ttcaaaatac
     1921 tagtgctcaa tgagatgctt gatttgatca caagaagact gcgaaagccg ctggcaaccg
     1981 caagctaggt atcccaccga tggactacgt tttagaggct actggttatg aatacggagc
     2041 caacgctctc atctgcataa gaatacatca cccagattat gtaaagattt tcgatacttc
     2101 tgtcaaaaaa tcattaagat agataaatga tttccgtcta gtcaaattgg ttcagaatgt
     2161 agacacacag atggtaagaa gtcactcaca attcgagaat ggacttgtcc tatttgccgt
     2221 atacatcata atcgaaacgt caacgatagt atcaatattt tgaccaaggg ctatgaatac
     2281 aagcattgcc ttagaaaaca tcaagaacct taggaacaac ggggatagct tggttaataa
     2341 gagataccgc tgtttaggct tgcaagtata ctcttttatc aagaattccg tggctttagt
     2401 catgagaggc ttaatactat gaaaattttc tttagcattt ttgatttagg ttccttttct
     2461 aattataata agatgaccac tacagtaacc tactgaaata tataaacagc cactgtagta
     2521 ccattttcaa agtagtctat gagaggagaa tcttcattct tatgaacgct ggaattgcgg
     2581 atatgaattt aataaaaaaa acccttaatg attttacttc caattcaata tcaaaaggta
     2641 ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg tgaaagatca gtggaaaagc
     2701 tgaatctttt agatgctata aaaataacgg aatttgctat gaaaaatgga aaagctgaga
     2761 ttgaaatatg gagataatta tcgtctagtg aattacttgc cacttagctg aatttaacga
     2821 tccttggtgc ttgaaggggg agattcttgg gaatagagca tacttgcaag cctatgtctt
     2881 tactaacaac gatatctctt attaaccaag ctatccccgt agttctaagg cttttacttt
     2941 tatctaagca ataatcgtat tcaaaggaaa tttttgaccc ttagaaagtc tcattttatc
     3001 agacgtgagc ttaggcattg ctgaattttg ttaattcggt tttaggttgt gctttgagca
     3061 taatatgaaa gtattcttta tcacgatttc attcatctaa ggtaatgtga tgtgattctg
     3121 aaattctttc aaaggcagtt tttgcataag ctgacattac atcatcaatg acatgtttac
     3181 aatgtttcac tactaaaata agggggtaac caagaagaaa aactgaatga ttatttgtat
     3241 ctaattccat ggtatatcaa ccatttccta tatagactga ttatgacatg gattaaaata
     3301 aaaaggcact atgtcttaat tgtcggtttt cgagttaccg aatggcattc atcacccacc
     3361 tatagaggat gggcgacttc tgcctgaaat aagttacact acttataact tctaataaaa
     3421 ttagattgag tcattattat ttaacatcaa cgattttcca gtttccattt ttttgtaata
     3481 ctaatttgta ctgaaagtag ctagtggttt ttgatacatt atcaaggtat tttatctttg
     3541 tactcactag aaaatttttt ccgttttgta gataaactgg attctctaac tccgaaaact
     3601 ttaagtcaat attgattggt cgcatcgcac cattttcaac ataatactcc aattcttttt
     3661 cagaagctga tggataaagt ttaaaaaatg tttctaaaaa atcggtgatt tcttccgttg
     3721 ttttggcatc aatttctgaa tcgttttcta cttgttttgc ttcatatttt gccttgtctg
     3781 gctttccaat cattgttggc aaactagtta ctaaatgatt cctgttttca tcttcaaaga
     3841 ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt tccctctgta atatgttgct
     3901 ccactaaaaa tgaaacgaca aattcttccg agtgtttttc aacatctaga atttttacag
     3961 attgaacttc ggaactcgtg ggaatatctg ctcgaaccat atcttggatt aaagcgagac
     4021 cgttttctgt aaggtactgt tcgagattac tcattctctt ttcaattgct tctttgttat
     4081 ttttccatga aaaatattct ttcgcaaaat cctctgtaaa actttcaacg ccagaagtat
     4141 tcactacctt ctcttttata accacttttt catgagttgt atgttgatca atggcggtga
     4201 aatttttata aacgccaaaa gcaagactac aagacaataa aagccaaagg gcaagcacca
     4261 tttttcgatg cttacccacg gatacaactc gttctttttt cttttttggg ctcttttcct
     4321 tttgattccg ttcaattttt atcttcattc tttttcctct tttctcttat ttgtatcggc
     4381 cagctccgat aagatgctgc cgccaatagt tttccgttag attcgtccac cccactggat
     4441 ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc cacatgggtc acataagtac
     4501 cggcatcata ggttgaatga aagaaaacta aatcacctgg tttggcatct tttagatcaa
     4561 tatgagtcat ggcatcatat tgatcctgag ccacccgagg aagtttcagg tcaactttgg
     4621 cataacacca ttgggtcaag ccactacaat caaaccccgt ttctggactt gagccaccaa
     4681 acacataggc cgttccttca tacttaaatg cctcatcaaa aatttcttgg actattttgt
     4741 catcaaactg agttgtagtt aggtattgct tcactaataa acagtaaaac atattgccat
     4801 aattataccg ccacccacca ttttccttaa tcgcgattgg gttggggtaa tcgacttttt
     4861 gaccacctga tttttccttt gaaaaagatt cagctaaagc aaaactatgc ttcttcccgt
     4921 ttttcccgac atgatctaga tacccgccac catagttata ggcctgaata atactatctt
     4981 gatcacaccc tagttctttg gaacgcttaa ccaaagacgc aaaatagaga cagccttgtt
     5041 tgatggatgc ctcggtactt aatgaattag gtggtaatcc ggctgactca gaactttgca
     5101 taacgtcttc cattgtgcca ccagattcca cttgaataat cgccaaaaga attggaatat
     5161 actctgttat atccgattct ttggcatact tttccaccat tgcacggtgt ttcaatactt
     5221 cttctgagac actagttccg ccaggagaag tgccagaact accatttccc tcactgtctt
     5281 caccaaacaa caaacccaca cacatcaaaa tacccataaa agagatggtc agaaaagtta
     5341 atgaaataac acccactttc ttcaagttca ttttttctca cctgaccttg attttggacg
     5401 atggttcatt cttttttgta ctttttgatt ggtctgcttt ggcagagtgg acctctgttt
     5461 cacttgttgg aaagaatttt tcttacttgc tgattggctt ttcccttttg aaatgatttc
     5521 ttgactagtt agttttcgtt tgatttcggg attcagattt gctctcacaa tccgctttgg
     5581 tgcttctttt ttcagcatca tatctttctg aggttgagct gctttatcta ttggaatatt
     5641 cgcccttctt tttccctgac gatttgttgc ttcactcagt atcttgcgtt tttcgtccat
     5701 tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc actcgatccg ctttttcttg
     5761 gaacagtccg cgcttgaatt cttctggtgc ttttttcgac ttttcgattc ctttatggag
     5821 attgtatttc aaattcgttg gcgtattcct tacctgatcc tttacttgtt tagtcttatc
     5881 tttcaccaaa gcttgggtat ccaaaacttt gccgattttt ctcccagcca aattcattcg
     5941 ggattgtttc ttattatttg aaactggatt ctccttagtt aagtcagata gtatttcgtg
     6001 atcgttcggt aatcgtgaat tctttcgtag agaagaaccg gatggcttaa atttggattt
     6061 tgattgttcc tttgccaact tatttccgac tgctgcacca ccaccaaaag ccaaagctcg
     6121 tcccacattt cgattgagtt gccgcaattt tcgattcatt aacatttgcg gttttcgcat
     6181 gacgcgtctt cccattgact ggctatcatt actctggagg ttgaacatac tcataatgtc
     6241 gcccaatttg aaatagatac cggcaaaggt cacaatttgc agaaaagcaa ccatgaaaaa
     6301 tggataggtt gccgaaatat tgaaaaacat cgtggagatg ctaaatgcag tcgtaatcac
     6361 caaagtaaca cccgctcgca tcataatcgt attgaataaa cgaataatcg ccttctttcc
     6421 taaactttca taagtcggca acatggacaa taagaaacta attggcagaa acatcgcaaa
     6481 gataatgaag agaatttgcg agaacaacat aatccctgtt agaagaaaaa caaagaagga
     6541 aatgccaata ttgaacaata ccaaaaagac cacggtccct aaacggttca tggtcttggt
     6601 aatactgaga ttcgcattgt cattgtcttc aatctctgcc ttgaccgctt cttcccgatc
     6661 tttgcctttg ttttcatctg gactcacaga tagaatcttg ttgacccggt cttcaccaat
     6721 ttcttctatg ttggaatcat caaattgcag tagtaaccaa ggttgttgga cttgaatcga
     6781 aaacaaacta tcccgaatca aatccacact atcttttcct tggctttctg aattgggtac
     6841 aacgatctcg gttccaaggg taagggccgc ttcactaaca tctgagctaa agtcattgat
     6901 ttttgtgata taggtaggcg cataagcaat gaacgaaccc gatagtagaa aaatcatcac
     6961 aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt tttaataatc cggtatacgc
     7021 cacatacacc cccagagcta aaatcatcaa taatagaaac ccaaaataaa atccggaggt
     7081 ctggagtcca ttttcagtaa taccagccaa tgtttggata ttctttccaa tactttcagc
     7141 cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc acataacccg tggcgttcga
     7201 caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca taaagaccat acatcacgct
     7261 tttccctaag ccatcacccc aattccaagg caaccaatcc caagagctat ccacaaagaa
     7321 atctagttga tagttgttca gtgaatattt ggaatataga tttcctgctt caactgtatc
     7381 atcaactagc cctgtcgcct cggctaccgt accaactaaa ctcaatagaa ctatcagagt
     7441 gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt ttctttttca tcccctcact
     7501 ccattctctc ttgaacaggt ggacgggtat caaaggcatg gaagagttct tcaaagactg
     7561 gatgtacctg aaccacgcct acccgaccat acaaatcctg catcaagcat tggccatttt
     7621 ccaaatctcg taggcgtttt tgattgcttt catcttctcg atccaagcca aagaattcca
     7681 aggttttctt gatttcatga atatcggtac tgcgaaacgc aaacttcaac ccgatattat
     7741 tcttcaagcg ttcatccagt aaatcatcac tattttgcgt gacaaagtaa accccggcat
     7801 tcatagcccg cccagcacga acgagcttat tcgataatgt tttcccttgc gctacttgaa
     7861 gaaaactcca cgcctcatct aagtctacaa ttttgaagat ttctcgatta ctatggataa
     7921 agtccaaggc aaaagtggaa atgacgatta gcatggaaac actaagcaat tccatggtgg
     7981 tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa atccgctact tgaatgatat
     8041 ttaactgcct atctaaacta atcgactggg taatcgaacc atctgagaaa agcaaatggg
     8101 caaaatcata atcggtaaag gactcgatgt gatccgcgat attttccgca actggtgagc
     8161 cttcttttcg aagctcatta attacttgta ataatccccg ttgattgctt tgggtaaccg
     8221 tccgaatggc cttacgcaac actggaaact tctccccatc tcgactagaa attcctgtta
     8281 aaaaggtcag aatatcaatt gctagacttt cagaatcctt ctttttctcc ataatcacat
     8341 aaggatcaag taatccttga ttttttggct tactagttaa gttgagaata ttgatttcat
     8401 ccgcaatttc tggcaaggtt tctttccagc caccccgttc cccttttgga tcgacaatca
     8461 cagcttgtcc accaaaaagg acggcgtaat aaactaagag attgttacta aaggactttc
     8521 cgccaccgag tgaaccgagg aatgccgctg ctagcgcatt ggtaactgaa tccttgaccc
     8581 cttgtgaggc caatgccggt ttcaggtaaa cattgcgccc cgtatctacg ttataaccaa
     8641 agtaaatgcc ttccaactcc cctaacatct gagtggcgcc aaaaccaagt ccggcgagaa
     8701 aatcagacgt aacatactgg atataatcat tcatgtatcg cttactagaa ggtaagaatt
     8761 cttcatgtag ccccatcata tcgccaaaag gccggactag ttttacattg gtatcatcat
     8821 aaaaatctag cacttcatca caccggcgct ttagttcatc tactgattcg gcacttactc
     8881 gaaccacata gctcagtttg tacatggatt ctttggactg atccaaggta gtttccaatt
     8941 catctacgga atccaatgca tctaagacat tggaatttgt ttcattgtca gattgatagg
     9001 catggttgtc caagtctttt aattcctttt tcttattgcg aacggtagca agtgcctttt
     9061 tgttcgtcac aatctccaca ttcatactag tgtcaattgg gaatgtaaat tgttgttgct
     9121 ggtaatagaa aagttctgag gagggaaact ccatttcccc cacaattgta ttaattgtta
     9181 agtacgctac atatgattca tgattctcat gttccatgca taagtaccgg ggcttttctt
     9241 caatcaaaca acgactggga cgtaataagt cgtaacgttt caccaaagtt tccgacttca
     9301 gctttttctt tggtagctga aattcatatt cttcaaatgg tgtacccttt tcgccataga
     9361 tatgttcaat caaatacatc agatcactcg gtgtgactcg tctgacttta aatcttcgag
     9421 ccaatttact ttccattagt ttttcaagct tggtatatcg gcgaatttct tcattggata
     9481 aggaaacaaa atcgcccatc aaatgatgat tcactccata gacaaattct tgaaagccgg
     9541 agaagaaaga tttcttgaga ttctttagat tgacctcttc atctgtcgca atcagtttga
     9601 agccaataaa aaagcgataa tcaatctgac tatcgccaat catggatact aatgcctctg
     9661 tctgtaaatc aattcgttgt ttcgctactt ctgttaatcg cccggtaatt tccttttttg
     9721 atcgttcttg cgtggcacga atactacttt ctgtggcaat ttgtaaggca tgaatcttgc
     9781 cttcacgatt ttgcgcaatc aattgacgaa agttatcgtg gacttgatat ttctgttcag
     9841 gtgacaaaaa ggagtaattg taaggtatta actcatagta ggcaaaacat tctccctctt
     9901 gattaaagac aagattattt tcaatatact taatcgggta catagttcac actccttacc
     9961 agagtaatgg gttcgtccca tcgtttcttc tgttctttga ctttctttcc tgcgaaagtg
    10021 accttaaatc gtaagtgata gctaataaaa gatcgtaaaa atcccaatgg tttcttaccg
    10081 tcaaacgttt tttgcgacat gaaccaagta actgccaccg gtatacctaa gtattttaaa
    10141 aaggccccct ctatcataga tagtggaggc acatgggcaa atacgatgac aaacagcaag
    10201 gataaaacaa accaagccat ctggttaaaa gtcacaggaa aaggcagttg aaagtcatta
    10261 attgcataaa tgaccttttc aactgcccaa atactggtgt agctttttat tttcttcatc
    10321 aaatcattcc tttaaaaaga aggatagttt agaaatcaac tacccttcaa taattttgtt
    10381 aatatggata ctcaaaaaca cctcgactcg ttaccaaata attgcctcca atctccaagt
    10441 ctctaccaca cgcatcaaaa tcaatatacg tcttcaagtg ttctggtact tctccaaaat
    10501 caccagcttc tacaatcaag aaatatgcta catcactcat atcatcgcaa tctggataat
    10561 agataatatc atccacatgc tctaccatct cctcaaaaga attgaagaaa gcatgttgaa
    10621 tctcagaagc aacttcacca actggcgttc cttcaagttc ctccgctaag ccacacaacc
    10681 gattaatttc ctcaattggc gtatactcat caatctcaaa gggaagttca taatcatgaa
    10741 ttgcatattc ctcgtattcg tcatttaacc caattcgttc tttcacttca tcaaaatcga
    10801 caggtggggt aaaccacgcc cccaccaatt caccttcgtt atatttcccg aggttcgcaa
    10861 tataaacacg catctgttcc atcgtgcttc ccccttacgc accgacaatt cggttaaaca
    10921 aactgagtaa gacatccttc accccagaag cattaaagac caatccgacc gcaatcaaag
    10981 caatcaccaa aaagccaatc agtttggaaa actcccgctt aaatcctaaa aacaaaccaa
    11041 tgaccacaat tgccagtaag actaaggatt g
//
LOCUS       DARDHA010000087        10928 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000087 DARDHA010000000
VERSION     DARDHA010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.088"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..287)
                     /locus_tag="SDA10_001817"
     CDS             complement(<1..287)
                     /locus_tag="SDA10_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4240718.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAE"
     gene            429..1547
                     /locus_tag="SDA10_001818"
     CDS             429..1547
                     /locus_tag="SDA10_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG4240719.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1675..3051
                     /locus_tag="SDA10_001819"
     CDS             1675..3051
                     /locus_tag="SDA10_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEG4240720.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3452..5032
                     /locus_tag="SDA10_001820"
     CDS             3452..5032
                     /locus_tag="SDA10_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEG4240721.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5230..5453
                     /locus_tag="SDA10_001821"
                     /pseudo
     CDS             5230..5453
                     /locus_tag="SDA10_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5658..5978)
                     /locus_tag="SDA10_001822"
     CDS             complement(5658..5978)
                     /locus_tag="SDA10_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEG4240722.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6126..8360)
                     /locus_tag="SDA10_001823"
     CDS             complement(6126..8360)
                     /locus_tag="SDA10_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4240723.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8839..9105
                     /locus_tag="SDA10_001824"
     CDS             8839..9105
                     /locus_tag="SDA10_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEG4240724.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9105..10832
                     /gene="ptsP"
                     /locus_tag="SDA10_001825"
     CDS             9105..10832
                     /gene="ptsP"
                     /locus_tag="SDA10_001825"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEG4240725.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3479 a         1912 c         2215 g         3322 t
ORIGIN      
        1 ttttctgctt cttcaatcaa tatttttcct aacccttggt gtctgtattc agaaagaatc
       61 gccatccgct gtaatttcat ttttccgttt tctaaaggca aaagacgtac agttccttgc
      121 ggttcttttt tatctgagta caggacgaag tggatacaat gggcttcatc tttgtctatt
      181 tctcgagaca aaggcacacc ctgttcgact acaaaaactt gatgcctgat ccgtacagca
      241 tctaagtaaa tattgctcat tgtatctttt gtttgtacaa ctttcatggt ctagccacct
      301 tcctttcgta tagtatactg gaaaataaaa caaataaaaa gtatggcaag ttttcaaaaa
      361 taaaaaaaga ataaaagaat ttttaacgaa tataatatat actagtaaga aagaaaggag
      421 gaaatctatt gtttgaaaaa cttagacagt ctaaactaat gttttggtcg cttgaattat
      481 tgatcatagc tgcattgatc atgatttcat cagaaataaa ctttattttc gaaccgatcg
      541 ggacattttt ttccacgtta tttgcacctg tactgattgc aggctttctt tactatttat
      601 tgaatcctat tgtcaagctg ttgatgaaag caaaaatcaa aagaatatgg gctgtcgcaa
      661 ttgtgctgct ccttttagtt gcagcaatta tttggatttt acttagtgtc atcccaagtc
      721 tagtacagca aatttcctca ttggcatcta atatgcctga tttcatcaaa caggtagaaa
      781 gctggttaaa agaggtagcc gaattgcctt tgttcaagga agtagatatc aataaatatt
      841 ttgagcaatt agacatttct tacgggacaa tcattcagca attccttagt ggagtatcca
      901 atagtttagg ttcgatcgtt ggaacgatcg cttcggcaac cgtagtaatc attacagcac
      961 cattcatctt gttctatatg ttgaaagacg gagaaaaact agtgccaaac atccaacagt
     1021 ttttcccaga aaaaagaaga aaccaaattg tggagttatt ggggcaactg aataaaacat
     1081 tggctaatta cattagcgga caagcaatcg aatgtttatt cgttggtacc ttcacatttt
     1141 taggatatat ggtaattggt gtggattatg ctttcttatt tggggtcatt gcgggattga
     1201 caaacctaat tccatattta ggaccttatc taggactagc acctgcattt ctagtaacag
     1261 tcttcaatga tccggtaaaa gcattacttt gttgtgtggt tgtgctaatc gtccaacagt
     1321 tggatgggaa tatcatttat cctaatgtaa ttgggaaatc actgaaaatc catccattaa
     1381 cgatcattat cgttcttcta gtagctggaa atattgccgg attgcttggt atcttcttag
     1441 gtgttccttt ttacgcaatc tgcaagacga tcatttccta tataatcaaa atcgtcaaag
     1501 aagacaagca aaatgaaaac aagaagaaaa ttgctacgac ctcgtaggta gttttagtac
     1561 acttgttttt attgcaaaga aaattgcttt gtgataccat tttaacgtgg ctatagtgcc
     1621 acgttttttt ctatcgtaaa ctaaaaatta attttataag gagagaatgc gattatgaat
     1681 gctgaccctg agagtcagtc gctgttagcg cagattttat tattgatcgt tttgacattg
     1741 atcaatgctt ttttagcagc ttccgaaatt gctgttgttt cgatcaataa gaatcgaatc
     1801 gagcaaaaag ctgatgaagg agatgtgaaa tctcaaaagc ttttaaaaat attgcagaat
     1861 ccaaataatt tcctatcgac aatccaagta ggaatcactt tggtcaacat tttgtctggt
     1921 gcttcactag ctaatacttt atcggaacgg ttagctccgg tattaggtgg cggcacagcg
     1981 gcaaaaaata ttgcaaatat cattgtgctg gctattttga cctatgtatc gattgttttt
     2041 ggagaattgt accctaaacg tattgcttta aataaatcag aagaagtcgc aaagttcact
     2101 tcagggctga tacgcttgat tggtgtagta gctaagccct tcgtctggct gctttctgct
     2161 tccacaagtt tattggcgcg tatcacgccg atgacttttg atgatgaaga ttcaaaaatg
     2221 accagagatg aaatgcgtta tatgcttgaa aacgaaggcg tattgaataa tgaagaatta
     2281 gaaatgctcc aaggggtttt ttctcttgat acgaaagttg cccgagaagt catggtccca
     2341 cgaacggatg catttatgat tgatatcaac gacactgtgg aagaaaatgt gaatgaagtt
     2401 ttatccgaaa actattcaag aatccctgtt tataacgaag acaaagataa agtagtcgga
     2461 atccttcata caaagaattt actaaaagct gcccataaat tcggctttga taatttagat
     2521 atcaagaaaa tcatgcagga gcctttattc gttcctgaaa cggtctttat tgatgatctg
     2581 ttatatgaaa tgaaaaagac gcagaatcag atggctattt tattagatga atatggcggc
     2641 gtcgttgggt tagcaacact agaagacctt ttagaagaaa tcgtcggaga aatcgatgat
     2701 gaatcagatg aagttgagaa tctttatgaa aaaatcgatg aacacgaata tatcatccaa
     2761 ggacgtatgc tgattgatga gttcaatgaa gcttttgaca gtgatctgca tatgagtgat
     2821 gtagatacga tggctggtta tttgattaca gcattaggca tgattccgga tgaaggcgaa
     2881 aagttatcgt ttgatgtcga taatattacg ctggtttctg aagaaatgga aggttctcga
     2941 atccttaaaa tccgtgtcat ttttcatgat ccggaagaaa cagaagcgga acctgaagaa
     3001 gaacgaagat acttcagaaa agaattcgaa gatgacgagc caagaagata attggaaact
     3061 tggttatgaa agacgtgttg cttgaataaa caagcaacac gtcttttctt ttagtcagct
     3121 gacaaaaaaa tatttgactc ttatggtttg gtggcttttt cattcgctga atccatgcta
     3181 tactgattca gttgtagtag ttgacaaagg ttgtgaaaaa gctatcctgc attaagtaat
     3241 aagagcagcc aagcaaaaac agcttttcgt atttttggct tgggtgggct tattgctgat
     3301 aggctgtctc tagaacgccg tttactcgga aaaagagact gctacacggc ttttgtcaca
     3361 gccctatatg aaattgtctt tctttaagag atcggacaat ctgtgcctag agaaaatata
     3421 aataattgaa tggaagaaat ggagttaggt tatgaataat ccaaaattaa agcaacaagt
     3481 ggacaatcgc agaacttttg cgatcatttc ccatccggat gctgggaaaa caacgattac
     3541 agaacaatta ctattgtttg gtggggctat ccgtcaagcc gggaccgtaa aaggcaaaaa
     3601 aacaggtaat tttgccaaat cagactggat ggaaatcgag aaacaacgtg ggatttctgt
     3661 tactagttct gttatgcagt ttgattatca agggaaacga gtaaatatct tggatacacc
     3721 aggacatgaa gatttttctg aagatactta tcgtactttg atggccgtgg atagtgcggt
     3781 catggtaatc gatagtgcaa aagggatcga agcacagaca aagaaattat tccaagttgt
     3841 aaaaaaacgt ggcatcccga tatttacttt tatcaacaaa ctagatcgtg atggtcgtga
     3901 accattggaa ttgttagaag aattagaaga gctattagat atcgaatctt atccgatgaa
     3961 ttggccaatc ggtatgggta aagggctaga aggtctttat gatatctacc ataatcgtgt
     4021 agagttttat cgtccggaaa attatcagga tgaacgatta gtagaattgg atgaagatgg
     4081 actgcttccc gaaaatcatc ctctaactga aaattcctta tatgaacaag tcttagaaga
     4141 agtagaacta atcaaagagg ctggagatgc atttaatcaa gaaaaaattg ctcgtggcga
     4201 tcagacacca gtattttttg gttctgcatt gactaacttc ggtgtacaga cttttttaga
     4261 gactttcgtt gaattggcac cagctcctta tggacacaaa acacaagatg atcagatggt
     4321 aagtccttat gaggaagaat tttcaggttt cgtattcaag atccaagcaa atatgaatcc
     4381 agcccatcgt gaccgaatcg cttttgtccg aatctgttct ggaacattcg aacgaggaat
     4441 ggatgtctgg ttggaacgta caaataaaaa actgaaatta agcaatgtca cacaatttat
     4501 ggcagattcg agagaaaacg tcgaaaaagc agtagcaggc gatatcatcg gtgtatatga
     4561 tacaggcaat tatcagattg gggatacatt gttcgaagga aaactaaaag ttgcttatga
     4621 agaactccct tcatttactc cagagctgtt tatgaaagtg actgcaaaaa atgtcatgaa
     4681 acaaaaatcc ttccacaaag gaatctatca attagttcaa gaaggagcga ttcagcttta
     4741 taaaacatat ctaacggaag aatatatcat tggagctgtt ggtcagctac aattcgaagt
     4801 attccaatat cgtatgttaa atgagtataa cgccgaagta atcatgtctc caatgggaca
     4861 caaaatcgct cgctggatcg atccagaaga tttggatgag aagatgagtt caagtcgcaa
     4921 tatcttggct cgtgaccgtt tcgaccaacc gttgttctta ttcgagaacc aatttgcaga
     4981 acgctggttt gcggataaat atcccaatgt aaaattaaaa agtttgatgt aaagaagtct
     5041 tgacacaagc tgccctgcat caagtaataa gaatagccaa acaaaaacag cctctcatat
     5101 tttgtttaga gccgtgacgg attttccgtg acggctcttt ttactatatt aaaatcatag
     5161 aaatgtctaa tcaaaaaaat ataaaaatta ttttgcgtac atatgattaa agaaaggaga
     5221 gtgtgacaca tggaaacaga atggcaaaga cgtgaaagaa aaaaacagaa aaagaagaga
     5281 tgttacgatt atgtcgatta ttagatgtaa aagactggga caaaaacgag caaattgcac
     5341 aaaaagtacg atcaatcatc cataaagagg aaaaagttaa aaataaagac acgcgtagaa
     5401 ctgaaaatga aaagagaaaa gtacaaaaga aaagagaaaa atcaaaaagc tagaaagaaa
     5461 aagaggctga gacagaagcg tttaactcca agaaataagc tggaattcac aaaaatttga
     5521 aaagcaattt tcgtgaattt cagcttattt ccgaaggagt tgcttctgct ttcgccgttt
     5581 atacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtcccacgc tctttaatta
     5641 ttatgttcaa aatttgttta cacggctaat gtagcgccta attcatgttt tggaatcgag
     5701 atttcaacaa ggcctgccaa ttcgattact ttgatttcat cctcgtggta aacggcgtct
     5761 ttgattttat ctaagaaata atttttagca tcttcgattt ctttcggctg gattttacgt
     5821 ttgtcattga tcagcagaat ttcattatgt tcaggggctt cagtgtagac aatagtggta
     5881 tttccggctg tgtagacctt gactagatgg gcatcagtgt tttcaagctg gtttaataca
     5941 agacgggaat ggctgtttgt tacatttacc aatttcatgg cgctcacctc ttttatgtat
     6001 ttttaattgc ttgtttctct tattataatt tcttttttat aaaatgaaca gtattttgac
     6061 ttttctgaaa aaagaatgag acggcatttc tcaaagaaat accgtctcac gctataaaac
     6121 tggatttatt cagccgtttc ttcggctgat tctttagcca aacgttctgg tttagaagta
     6181 acgatgattt ttccatcttt atccagtttt gctttcaact catggataga agggtgatca
     6241 agataaaatt cagcgatacc atcttcgatt tgttcttgga tcgtgcgtcg aagaggacga
     6301 gcacccatag atggatcata gcctaagtca actaattttt ctttgacatt cgtaggaact
     6361 tcgatgtgaa gttgctgtgc tgccagcatt tggttgacat cgtctagcat tagtgtgacg
     6421 attgtcatca agttttcttt tgataatgca ctgaattcga tgatcccgtc aaaacggttc
     6481 agaaattctg gtgtaaagta gttgtttaat tggttcaata cagaacgagt aacaccttcg
     6541 cgtgccgcac caaaaccaac gtttgcttct actttaccag ttccggcatt acttgtcata
     6601 atgatgatcg tatctttaaa gctgaccgtt cttccttgtg catctgtcaa tcgaccatca
     6661 tctaagattt gcaagaacat atgcaaaaca tctggatgag ctttttctac ttcatctaac
     6721 aagacaagac tgtaaggatt tctccgtact ttttctgtta attgaccagc ttcttcgtat
     6781 ccgacataac ctggaggaga accaatcaat ttagagacac tatgtttttc catatattca
     6841 gacatatcga aacgaatcat tgaatcttgt gaaccgaata gttcgtaggc aagttgtttc
     6901 gcaagctctg ttttacctac accagttggt ccaacaaata agaaggagcc gattggacga
     6961 ttctgtttgt ttaatccaac acggtttcgg cgaatcgctt tcgctactcg atctacagcg
     7021 ttatcttgtc cgatgacatg agctttcaag tcatctgcta agtttttcag ttgtgtctgt
     7081 tctttttctt ttaattcacc cactggaata cctgtccgtt gttcaacgat tttttccata
     7141 tctttttcgg taatcacagg tgtttcctct tcagtgagct gacgttcttt cattttttga
     7201 agtttattga tctgatcgcg ataataagct gctttttcga aatcttcttc tcgagaagct
     7261 aaaacttttt gttcttctgc ttcttgcagt tttttctcga ttgttttagg atcaacgatt
     7321 tggatcgtca agtttttctt agaacctgtt tcatctagca aatcgatggc tttatccggc
     7381 aagaagcgat cttggacgta acgattggat aaagttgcgg ctgcttcgat ggcctcatca
     7441 gtatatttta catgatgata gtcttcataa cgtttttgca aacctttcaa aatactgatc
     7501 gtttcttcga cagttggttc atctacccgt acaggttgca tacgacgttc taaagcagcg
     7561 tctttttcga tgatgcggta ctcatttaag gtagtagcac caaccatttg caattctcct
     7621 cgagctaaag ctggttttaa aatattacca gcatccatat ttccatcacc agcagaacca
     7681 gcaccgacga tttcgtgaac ttcatctatg aataggatca cattttctgc ttgacggatc
     7741 tcttcaatca atttttgcat acgttcttca aattgtcctc gtataccagt accttggact
     7801 aaagatacga catctaaacg aatgacttct ttgtccatta atttttgcgg tacatcacca
     7861 tctacgattt tttgtgctaa accttcaaca actgctgttt taccaacacc tggttctcca
     7921 atcaagacag gattgttctt ggttcggcga tttaaaattt caatgactcg tttgatttcg
     7981 tcatcgcgtc cgatcacagg atcgatatca ccttgtcgtg cttgttctgt gatattgatt
     8041 ccgtattctc ccaataaacc ttctgtattg ccttgaggtg gctgaccacc attgaagttg
     8101 ttgtttccgc caaattgtgt tggtggtgtt tgctcataat ttccttgctg ttgcatttgg
     8161 cgagacatgg aacggaaaag atcgtccaaa ctgccaaatc caaatggatc attttgggcc
     8221 atattagtca gacctccatt ttgttgattt ttgatttttt gataacagct ctgacagtaa
     8281 tcgagttgtg tccgctgacc attgacagtg gcatacaagt gaatcgttgc ctcattcttg
     8341 ccgcagtttt gacaaagcat gaatcattca cgtcctttca caaaaatggg tacatccata
     8401 ttttacatct cgtttttcat gaggtaaaag aataacccct agttttttaa gagatttatg
     8461 aggtagaaaa ggtgaaatct ctattgacca ttcctgacct ttgactctta ttatactaaa
     8521 aataaaaagg gaagcaagta attcgtttca tatgaaaaca aagagggtca aaggaaagaa
     8581 tgataaacgt caggagaaga gaaagcgaca agaaaaagga aataaacacg tttgttataa
     8641 aatgtgaaga aaaggatgaa aaagaatccg gaataatttg aatactttta tctgaaattg
     8701 aatgaaaact gttgtatcta acgaaaaaaa atgttaatct ttacagatga aagggtggca
     8761 tttgttagga ttctctgaat tactaaaaaa gaaaaccctc acataaaaac aacactcact
     8821 taaaggagac cgattaatat ggaaaagaaa gaatttcacg tagtagcaga aactgggatc
     8881 cacgctcgtc cagctacact attagtacaa acagcaagca aatttaactc tgatgtaaac
     8941 ttagagtata aaggtaaatc agtaaacttg aaatcaatca tgggcgttat gtctttaggt
     9001 gtaggtcaag gttctgatgt tactattact gcagaaggtg ctgacgaagc tgatgcaatg
     9061 gcagcaatcg ttgaaacaat gaagaaagaa ggcttatctg aataatggtt gaaatgctaa
     9121 aagggattgc cgctagtgac ggtgtagccg ttgcaaaagc ttacctgcta gttcaaccgg
     9181 atttaacatt cagtaaggca acagtagaag atactgcggc tgaagaagct cgtttggatg
     9241 ctgcattagc taaatctact gaagaattgc agcaaattcg tgaaaaagca gcgcaaagct
     9301 taggtgaagc agaagcgcaa gtatttgatg cccatttgat ggttctttca gatccagaaa
     9361 tggtaggtca aatcaagcaa aatattaaag acaacagcgt aaatgctgaa tcagcgctaa
     9421 aagaagtaac ggatatgtat atcggtatgt tcgaagcaat ggaagataat gcttatatgc
     9481 aagagcgtgc tgcagatatt cgtgacgttg ctaaacgtat tttagcgcac ttattaggcg
     9541 ttactttacc aaatccatca atgatcaacg aagaagtggt cgttgttgca catgacttga
     9601 ctccaagtga tacagcacaa ttagatcgca attttgtaaa agcgtttgta acagacattg
     9661 gtggacgtac ttcacattct gctatcatgg cacgttcttt agaaattcca gcaatcgttg
     9721 ggacaaaaga aattactgct aaagtcaaag aaggcgatat tttggctgtc aacgggatcg
     9781 aaggcgatgt aatcatcgat cctacagatg aacaaaaagc tgaatttgaa aaagcaggtg
     9841 cagattacgc agcacaaaaa gcagaatggg aaaaattaaa aaatgcagaa actgtcacag
     9901 ctgacggcaa gcattttgaa ttagctgcaa acatcggaac accaaaagat ctagttggtg
     9961 tccataataa tggtggagaa gcagttggtc tgtaccgtac tgaattcctg tatatggatt
    10021 ctccagactt cccaacagaa gatgaccaat atgaagctta caaagctgta ttagaaggta
    10081 tggaaggaaa acctgtagtt gttcgtacaa tggacatcgg tggagataaa gaactaccat
    10141 atctgcaatt acctcatgaa atgaatccat tcttaggata tcgtgcattg cgtatcagtt
    10201 tatctgaaca aggcgacgaa atgttccgta cacaaatgcg tgcattgctt cgtgcatctg
    10261 ttcatggtaa tttacgtatc atgttcccta tggtagctac actcaaagaa ttccgcgcag
    10321 caaaagcgat ctttgaagaa gaaaaacaaa aattgataag tgaaggcaag gaagtatctg
    10381 atacgatcca agtcggcatc atgatcgaga ttccagcagc agcagtctta gcagacaagt
    10441 ttgcaaaaga agtagatttc ttctctgtag gtacgaacga tttgattcaa tacacaatgg
    10501 cagcagaccg tatgaacgaa cgcgtttctt acttgtacca accatataac ccatcaattt
    10561 tacgtttgat caaaaatgta attgatgcag cacacgctga aggaaaatgg gcaggtatgt
    10621 gtggagaaat ggctggagat caaacagctg ttccattgct agtaggtatg ggtctagatg
    10681 agttctcaat gagtgcgaca tctattctta aaacgcgtag tttaatgaaa cgcttggaca
    10741 cagcgaaaat ggctgaactt gctgatcgtg cattgaaaga atgcgatatg atggaagaag
    10801 tcgttgaact tgttcatgaa tatgtaaaat aaacaacgta tcaaaagctg gtgtggcttg
    10861 taaaagagct acgccagttt ttttgttctt ttttaagaaa acgtttttgt cagtaagaat
    10921 agacaaaa
//
LOCUS       DARDHA010000088        10807 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000088 DARDHA010000000
VERSION     DARDHA010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.089"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(25..162)
                     /locus_tag="SDA10_001826"
     CDS             complement(25..162)
                     /locus_tag="SDA10_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEG4240726.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="SDA10_001827"
     CDS             complement(159..1343)
                     /locus_tag="SDA10_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEG4240727.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="SDA10_001828"
     CDS             complement(1599..1853)
                     /locus_tag="SDA10_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240728.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="SDA10_001829"
     CDS             complement(1878..3020)
                     /locus_tag="SDA10_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEG4240729.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="SDA10_001830"
     CDS             complement(3080..4432)
                     /locus_tag="SDA10_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG4240730.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="SDA10_001831"
     CDS             complement(4503..4847)
                     /locus_tag="SDA10_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEG4240731.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="SDA10_001832"
     CDS             complement(4857..5231)
                     /locus_tag="SDA10_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG4240732.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="SDA10_001833"
     CDS             complement(5244..5558)
                     /locus_tag="SDA10_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG4240733.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="SDA10_001834"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="SDA10_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="SDA10_001835"
     CDS             complement(8976..9638)
                     /locus_tag="SDA10_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240734.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="SDA10_001836"
     CDS             10255..10485
                     /locus_tag="SDA10_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240735.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            10491..10790
                     /locus_tag="SDA10_001837"
     CDS             10491..10790
                     /locus_tag="SDA10_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240736.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT         2997 a         2384 c         1832 g         3594 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
    10741 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa ataaaaaaat
    10801 aacaaac
//
LOCUS       DARDHA010000089        10624 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000089 DARDHA010000000
VERSION     DARDHA010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.090"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            145..2205
                     /locus_tag="SDA10_001838"
     CDS             145..2205
                     /locus_tag="SDA10_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEG4240737.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2288..2857
                     /locus_tag="SDA10_001839"
     CDS             2288..2857
                     /locus_tag="SDA10_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4240738.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3032..3838)
                     /locus_tag="SDA10_001840"
     CDS             complement(3032..3838)
                     /locus_tag="SDA10_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240739.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3976..5490
                     /locus_tag="SDA10_001841"
     CDS             3976..5490
                     /locus_tag="SDA10_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEG4240740.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5893..6876)
                     /locus_tag="SDA10_001842"
     CDS             complement(5893..6876)
                     /locus_tag="SDA10_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEG4240741.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6881..7528)
                     /locus_tag="SDA10_001843"
     CDS             complement(6881..7528)
                     /locus_tag="SDA10_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG4240742.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7613..8245)
                     /locus_tag="SDA10_001844"
     CDS             complement(7613..8245)
                     /locus_tag="SDA10_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG4240743.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8261..8812)
                     /gene="coaC"
                     /locus_tag="SDA10_001845"
     CDS             complement(8261..8812)
                     /gene="coaC"
                     /locus_tag="SDA10_001845"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEG4240744.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8809..9546)
                     /locus_tag="SDA10_001846"
     CDS             complement(8809..9546)
                     /locus_tag="SDA10_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEG4240745.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9543..10532)
                     /locus_tag="SDA10_001847"
     CDS             complement(9543..10532)
                     /locus_tag="SDA10_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEG4240746.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
BASE COUNT         3426 a         2007 c         2113 g         3078 t
ORIGIN      
        1 attccgattt tcttgaaaca ataaaaaatc aaaaaaaata ctctcctaat agtccatctt
       61 cttttgattt tatttatata atgaaatcat cttatgaaaa ttactacttc atgtttagac
      121 aagagtaggg aggactaaga ataaatgtgt tcaaaaagac gttcaaaaca actaaataaa
      181 cacgcttgtc gtttagataa ggaacggtcg tgttctgaaa aacactacgt taagtttatg
      241 atgcgcacga tgctgatcag tttatctgta ggtgtctata tgcatgtacc cgttcaggca
      301 acgacacaaa ccatcaaggt cgtacgtacc tctgtttctg agaccactga ttcccctatc
      361 actattactc atactttcac tgatgcaaat ggaaaacagc tacttcctgc attgacttcc
      421 tataataaag tcagttatcc tccaacgata aaccactact ggttaattcc taacaaaaca
      481 ttatggacat cggaaaccgg aagcagtgtg ccgctttcta tcttttccga tatgactgat
      541 cctacaaaag gtgatcagtt aaaacagatc actgatattt tgaatcatgc tttgacagat
      601 ataaaggacg gtggaagcgc acaagtttct tatgtatatg aaaaagatta ttcacagttt
      661 gaaagaaaga aaatcgatgt tctggcaggg acaaaagttt cgatatctga tctggtagaa
      721 aggctgaaga acgcagatgg aacacaagga gatatgtcca aagtaatttc aaacaatggg
      781 aatatcgtgg atacgactaa tacaggaaca tctactattt ccttgtccta ttttgatcca
      841 attgctttaa aaacaatgac tgcatctgct gtgatccatg ttcgtgctga tcaaacagct
      901 atcaaaggaa aagatcttca cttaagaaca ggagagagtt acgcccctaa agatcttgtt
      961 gaatatgtaa caaaaagcga tggaacttca ggaaataaag aagaaatcaa aatgaacggg
     1021 acagtagata ctacaactcc gggagaatat cccgttgtat taacttatat agactcgaaa
     1081 acatcaaatg tggcagaaac aacagctgta gtttatgtag aagcatctgc tgcttcatcg
     1141 actgtcatca ctcattttat agatagtcaa acaaataaag aacttcaagc agaaacacat
     1201 atgagtagtg taaatcgtat tcctgatttg cgcctacctg ctgaattttc tgatcgagtg
     1261 ccaaatatag atttatcgac ttatgaacaa aatgggaaag tcacttctat ttctgattgg
     1321 atgcaagcaa acggattaga cagaaaaagt cactggtcgg agatccttga agtacttcac
     1381 tcagaactga tgaataatag gaagcaatca ggaggggaaa ttacctttac ttatgtttat
     1441 ataccagaca atcgttcatt agaaggtcat gatcttattg ttcatccaaa cgaaaaaata
     1501 aacaaagaag agttgatcca atcagcaaca aattccttag gtaaacccgt accgttaaag
     1561 gatgtatcgg ttacaatcaa tgaagtacca ttggaggatg gttatttgac caaacaaaca
     1621 ggtgatttta cgattatgta tagttatcat gacccgtatt cactccaaga actccatgct
     1681 cagtcactat tacatgtttt accattaaac aaacagatag atcaaaaaga acaaaaaaga
     1741 cgagaagaaa gcttgtcaga aaaaaaacaa caaatggctc atgcagtttc tccaacaggg
     1801 aaagtaaaac aaacaagaag cctagagtta gcaagaatat ctagaaaaat ctcagaatct
     1861 aataaaacga taaggcaaga agctcatttg gccccacaag tttctggatt ggaaaatgaa
     1921 attttagagc ctaaaataaa acaacaatca gaaaaagaat ttgttgctaa acagtcggaa
     1981 gatcagaagg aatcagatga ccagaaggaa acgaagaaaa aacgcttgtc aactattgat
     2041 tccaccgatg agcatctgca gccgcaagta ccacttccta gtccgggaac acccgcacct
     2101 ggtggaggta ttccttcttc tgctttagga gatttcatgc gaggtatggc aggaacatgg
     2161 gtatacgcta atcgaaatga atttgacgca gatgactggt tctgatttat atctctatct
     2221 gttatagtca aataagtagc tgagtcatat gttatttggc ataatggaga cgaggaggga
     2281 tagaaaaatg ttagataaaa cgttacctta cgcagaaatc tggatgacaa gaccacttca
     2341 acaaaatgtt ccaatcacgc ctttagcgga aggttttgaa tttcgatttt atcaaaaaga
     2401 agatgaatct gcttgggcgg agattgagac ttccgttggg gaatttcaaa cgacagatga
     2461 agcacttgag tattttcaac aaaactttgc tccttatcct gaagaacttg aaagaaggat
     2521 gttttttgta gagacaaaaa aaggagaaaa agtagcaacc tgtaccgctt ggcgaaaaaa
     2581 atcaagagag caagtgtatc cggtttttca ttggttagca gtgaaaccag attaccaagg
     2641 aaaagggctc gcaaaagcat tgactgccag agtcttgcaa gaatttccta ccctccatac
     2701 ggacggacca atctatcttc acacacagac atggagccat caggcgattg ctctttataa
     2761 aaaaatgggt tttactttta ttgctgaaaa tctagacggc acacctaatc cagattatga
     2821 aaaagttatg caggttttaa gtaaattaga taaataacga aatggtgaaa taagtcctca
     2881 gtattgggat aaaaaacgaa atagtcattc ctcatatctt acttttttga gttaatacta
     2941 tcggataact aatggcatgc catacattca aaaaagaagg ggcagtgaca aaagtattgt
     3001 cactgcccct tctcttgttt tttagcatgt tttatcgttt gattttttta ggaataaaat
     3061 tcttgatgaa cgtaaatttt tggtaatcgt ttcgactata gaggtaacca aatacactga
     3121 aaacaactgc tccaataaaa ttaacaaata aatccttcat agtatccatg attcccacgt
     3181 ctaagtaacc attttggatg attgttttat ctgttttacc atctgcattt tgactttcaa
     3241 tgattgtccg agtgatatct ggaacatgat agacagtgtt tccgtcttta cttaactcca
     3301 cactattgat ttgctggatg atcgtatctt tttgcatatc gaattgcagt aatttatctg
     3361 ctgaaaattc cacaaactcc cacataacac caactgtcat cgaaaaacag aaggaaacaa
     3421 ctgctagtaa taaaggagtc aagttcatgg tttttgtatt tttattgagc aaataaacaa
     3481 gggaaaagcc gacaccccca gctaaaaatc cgctgaatgc atgtagaaaa gtatcccaca
     3541 ttgaaaaata gccataaaaa tcacttaact ctcctaaaac tgttgaacta aaaataaaag
     3601 ataacaaaat gatttctagc atatttggaa tctgtatctt taatatcttt tccagaaaat
     3661 aagggatttc aaataaaatt aatgtcagta ataaaagcat tgtatttccc cagtgttggt
     3721 tccagacctg atccactagc ataataacga cgatgatacg taataaaata tagattccgt
     3781 ttgtgattct acctgcagtt tttgtactgt tcgtaaaaaa ggttttcaaa tgattcataa
     3841 aataactcct tcatgtatat ccttaattat actaagaagc gtgtgtttac caaagctata
     3901 tgacttaaag aaaaacagag ttgatattgt gaaaaaaatc acttaatata gtataaggag
     3961 aaaaggggga tacaaatgaa aaaattagtt gctggtttat taacagccgt tgcacttctt
     4021 gctatgagcg catgcggcgc agatcagaaa aatacagcgc agacaagttc tagtacgaag
     4081 gaatctgccg aagtatcatc aggcgcttct gaacaagtat atacagatcc aaaagaaatg
     4141 aaagaatctt atgatgtcgt gattattgga gctggtggtg cagggatggc agcagctatc
     4201 gaagcaaaag atgctgggtt gaatccagta attttggaaa agatgcctgt tgccggagga
     4261 aacacatcta aatcatcaag cggtatgaat gcttcagaaa caaaatttca aaaagaagaa
     4321 ggaatcaaag actcgaatga tgccttctat gaagaaacgc taaaaggtgg tggaggaacg
     4381 aatgataaag aaatgcttcg atactttgtc gatcactctg ctgatgcggt cgattggcta
     4441 gatcaaaacg gtatcaaact ggacaactta acgatcactg gagggatgag tgaaaagagg
     4501 acacatcgtc cgtctgatgg ctcggctatt ggtggatact tagtggaggg gttattgaat
     4561 aacgtccatg agcgggaaat ccctattttt gtcaacgcag atgtgacaga cattaagaaa
     4621 aatggggaaa cggtcaatga agtgactgta catgttcaag gagaagaacc aaaagaggtc
     4681 accggaaaag caatcgtagt taccacaggc ggttttggtg ccagcaaaga atttattgaa
     4741 aaatatcgtc ccgatttagc tgattatatt acgacaaatc aagaaggatc aactggagat
     4801 ggtatcacca tgattgaaaa attaggtgga caaaccgttg atatggataa aatacaaatt
     4861 catccaactg tgcagcagga tgagggggtc ttgattggtg aggttgttcg cggagaagga
     4921 gcaatcttgg tcgatcaaga ggggaaaaga tttgtcaatg aaatggatac aagggataaa
     4981 gtatctgcag caattacagc attgcctgaa aaatcagctt atttgatttt tgaccaaggc
     5041 gtgcgtgatc gtgccacagc tattgaattt tatgaccaaa aaggatatgt cacaactgga
     5101 aagacaatgg aagaattagc agaaaaaatc gatttaccaa aagaaacact agcaaaaaca
     5161 gttgatggat ggaatacgga tgttgcggag aaaaaagatg ctcaattcaa ccgtgccaca
     5221 gccatggaac atgacttatc gtctccgaat tattatgcga tcaaaatcgc accaggaatc
     5281 cattacacga tgggcggtgt gaaaatcaat acgaacactg aagtattaga taaagaaaac
     5341 cagccaatta gggggttata tgctgctggt gaagtcacag gtggcttaca tggaaacaac
     5401 cggatcggtg gaaattcagt tgcggagatc attatttttg ggcgacaagc gggcatacaa
     5461 gcagctcgat ttgcttcgga aaatgaatga aatagaacgt aaataaaaag aggttgtgaa
     5521 atcagcgttt tgagctaata ccgagggtca gctgattgaa cactgtttag tgaagaggtt
     5581 gtgaaaaagc tgccctgcat caagtaataa gaacagccaa gtaaaaacag cttcttatgt
     5641 ttttaacttg gttggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattcggaa
     5701 aaagagcgtg ggacaaaagt aaagaacact tttgtctcac gctctaaaca cgtataaacg
     5761 gcggaagcag aagaaattcc ttcggaaata agctgaaatt cacaaaaatt tgaaaagcaa
     5821 ttttcgtgaa tttcttctta tttcttggag ttaaacgctt ctgtcccagc ctcttttttg
     5881 atcagcatga ttttattcag ttatcttcaa aatgattttt ccgatgtttt tgtttgcttc
     5941 catatattca tgcgcttgtt tgactgcgct gaattggaat gtacggtcaa tgattggccg
     6001 aatcttgcca ttttctagaa aaggcccagc tttttgcata aaagtttgtg cgagctctgc
     6061 tttatactga tcacttctag gtgtcaataa tgtaccaagc agttggacac gtttcccaat
     6121 caatacacca aggtctatct caggcacgat ggtcccacct agcataccaa tcaagaccca
     6181 acgtccatca acggctatag aagctaaatt cttttcccag taagaagcgc cgatgaaatc
     6241 caaaataaca tcgactcccc gaccttcagt gatttttcgt acctcttctt gaaagtcttg
     6301 ttttttatag ttgatcaatt catctgcacc aagttcttta caaaaggcga gtttttcttc
     6361 tgatcctgca gtaacgatga ttttcgcgtg agaaagctgt ctagctaatt ggatcgctgc
     6421 tgttcccaca ccacttgctc ctgcatggat caagacagtt tcgccttctt gtaattttcc
     6481 aagccagtaa agtgtttgat aagcagtcaa aaaaacttct gagattccag cagcagaaac
     6541 atagtctagc gtatcaggga gaaggattgc acgatcggca ggcatcacag catattcggc
     6601 ataaccacct agattgacta atccgtatac ttttgtccca ggagtaaaag tggagatatc
     6661 acttttattc tcaactacct ctccagctac ttcgactccc aaaatagggt agggaggttt
     6721 caaagagact gattctcttt tcataatatc tgttcgattg acagctgctg tatggacttt
     6781 gatcagtaat tcgccttgtt ttggtttagg aacaggcact tcaatttgtt ctaatacatc
     6841 tacagaacca ggatgcttga ttgaaatggc acgcatggat ctattcctcc tttgcccatt
     6901 cttggaatgc tgtattcaac tctttgctac cttcagaaag tactagagtc gtacgagaaa
     6961 tatccgtttc ttccactaga gcggcaagta cagcagcaac tgtagagcga gtgatcgttt
     7021 ttgatgcctt ctcattaggc ttagttaatt gaatggaagt aacttcttca tcgttagtca
     7081 atgtgactgg ttgaagaatg acaaaatcta agttagttcg tttcagccat tcatctgcat
     7141 aatgtttagt aatataataa tctgtcatgg actcaggcca ttttgctggt tcatctgcat
     7201 aaacagcact gaccatcaga tagcgagaga catgcgcatt ttcagctgcg atcactgttt
     7261 tgattgcgcc atccaagtct acttgcagaa gattctttcc ttgagagccg gcagcaaaaa
     7321 tcaatacatc gatgcctttg aatgcttcgg tcattttttc cacggaccaa gttaaatcaa
     7381 aagagacata cgaaacattc tcttttttta ctaatgtttg ggaagcaaca tctctcaccc
     7441 ctgcaaaaat ttcatgggaa cttggagcta aatcttttat caggtgtcgg ccaatttgtc
     7501 cgtttgcacc tacaacaaaa attttcattt ggatcatcct ttcgacgtca aatctttcca
     7561 ttcaatagta acaccaaaag agaaaaaaat cagagatcgg ctacgatgac tatcagttag
     7621 taagagaagg gtgcttgaag tatcttaaca agacattgca tactgctgca gtcgcaagac
     7681 ctgcgacgat tccttcaggt ataccacttg taaaaataac cgctagaata gctgaataaa
     7741 cagcatttaa atttgttccg atgactttgg cataatcttg ctggaataaa aagtagatca
     7801 tattcatcac taaaatcgta ttggtcaatg aaccggaaag tccagctaag aataaagaaa
     7861 cggaatctgc tttttgtttc attagtttgc gcagaccttt ataaacgaaa taaggaacca
     7921 ccccaatcaa gatgcgagga ataaacgcga tcagcaaggc tttccagctt ccatgttctg
     7981 ttccgatgac tgggataaac ggagaaaaga caaaagacaa aggtgtttgg ataactgtgc
     8041 tacggatcat gctgatcagt ccaaatgtac cgccaagaaa agcacctagt cttggtccta
     8101 aaacgatcga agcaatgatt acaggaatat gcatggttgt tgcattgatt ggaccgatag
     8161 ggataaaacc taaaggcgta acagctaata aaatcaaaat agctaaaaac atcgcagtta
     8221 atgtaaagtt ttttgtattt ttcataagta ctcctttttt ctaagcattt ctttcattaa
     8281 gagtcagcat gacctgttcg atgatcgtct gaatatcagc taaggcacct tttccaaaat
     8341 ctccacaagc taaaagagat tctcttggtt caatttcctg atagccgaca gatctcaatg
     8401 tttccagatt acgctgagtg atcgggtttt gatacatatt cgtgttcatg gcaggggcaa
     8461 tcaattttgg tgttcccact ggaagcgcca ttgcgactgt tgaaagaaga tcgtctgcaa
     8521 tgccgtttgc tagcttgccg ataatgtttg ctgtagcagg cgcgatgaga aacaagtcag
     8581 cttgttttgc tagttcgata tgattgatta cctgcggatt tgattcttgc atgacgtctg
     8641 tatggactgc gcgcttcgac agagattgca aggttaatgg cgtgataaaa tgtgtgctgc
     8701 ttttagacat aatcacatct acctgggcac ctaatttgac gaattgactt gtaagatcag
     8761 cgcttttgta agcagaaata cttccagtaa cgcctagtaa gatctttttc attttgattc
     8821 ctccagtcta attttctcag taatcaatgc agcaatctca gatttacttt gagcttcttc
     8881 cactgttcca tctttagata agagatagcc gtggtgtgtc tcgccatgga tttccgtcaa
     8941 atcatttgcg aaaataaaat cagttttgtt atttatcaga ctattttttg cgactgccag
     9001 aagagattcc tttggtacat ctactaatag tttgaaacca atgatttttg cttttggctg
     9061 ccattcacgt agataagaaa tgatttttgg tgttttcttc aaaacgagca caagatgctc
     9121 ggtgttagaa gagatctttg aaacagtggt atctgtctgt tcattttccg caaaccaatt
     9181 gtccagttct tcttgcgttg aagaagtggg taggtttttt gccagctgtt cttctgaaaa
     9241 actaaaagca ggagtaaaat cactgactgc catactgtgg ataatcgcat cgtagtgttc
     9301 ttttttcgtt agttcttcta attgtaaaaa taagtccagt gcagattcga tcgtataaat
     9361 cgttatattg gagtgtcttt tcggtttcag tgcttgtttc gtagtcacgt aatcgattgt
     9421 ggtatccgga ttggcagcta aataatcagc taatgcttgc cctaatcgtc ctgtcgaatg
     9481 atttgtaata gcacgtactt gatcgatttt ttcagtcgtt cctccagctg tgattagaat
     9541 cttcatttat ggtcacctac atttactagt gttcttcctt ggtgtgttcc agcaagtagt
     9601 ttagatgctg tttcaggcaa ttgatccaga gtaatctctt ggacgaccag ttcatctgca
     9661 atctgcaaat ccgccatctg ttgccataaa gaaagacgtt gatctattgg tacattgaca
     9721 gaatcaattc caatcaactg gatgtttcgc agaataaaag gcaagacagt cgcgtttagt
     9781 ttgattcccc cagcattgcc acataaagct actgcaccgt tataagagat caatggaaga
     9841 agactgctta gctgctcgcc acctactgtg tcgatgacat agtcgatttg ctgttttcct
     9901 aaagcttttg ttgtctctgg cagaaattca tcaggtcgta cgacttgcaa agcacctaga
     9961 ctttttagcc atgtgacaga ctcttctttt ctggaaaagg caatgattga ctggaatcct
    10021 agtttatgta acagggctat cgctgtactg cctacaccgc cagaagcacc agtaacgaca
    10081 acttttttat cggaggtcat accttgacgg attaaagcgt tcacacacaa catagcagta
    10141 aaccctgctg taccaaggat catcgcttga cgcagcgtca tattttttgg tagaggaact
    10201 aaccagtctc ctggtacttt ttgatattgg ctataaccgc ctggatgggt gacgccaagc
    10261 ccaaaaccag tagcaagtac aagatcttct tttgaaaaac gtggattgtt actttggacg
    10321 acagttccgg ataaatcaat ccctggagtc atcggatatg ttcgtatcac ctgaccagac
    10381 tcgctcattg ccaatgcatc tttataattg acatcagaat aggcaatttt taccaagact
    10441 tcattttcag ccaactcagt aaatggcaga tcactgattg ctgtatggaa aacaggttcc
    10501 tttattacct gaaaagatcg aaaagcgttc attcgtgaga cactcctttt aaaattttct
    10561 tttatcatac cataaagcct actcttttat tcttgttctt ccgtaaattt agacaaaaat
    10621 tagg
//
LOCUS       DARDHA010000090        10458 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000090 DARDHA010000000
VERSION     DARDHA010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.091"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(159..644)
                     /locus_tag="SDA10_001848"
     CDS             complement(159..644)
                     /locus_tag="SDA10_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEG4240747.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    734..1079
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1124..1765
                     /locus_tag="SDA10_001849"
     CDS             1124..1765
                     /locus_tag="SDA10_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG4240748.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1784..2419
                     /locus_tag="SDA10_001850"
     CDS             1784..2419
                     /locus_tag="SDA10_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG4240749.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2437..3252
                     /locus_tag="SDA10_001851"
     CDS             2437..3252
                     /locus_tag="SDA10_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG4240750.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3571..5007
                     /locus_tag="SDA10_001852"
     CDS             3571..5007
                     /locus_tag="SDA10_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG4240751.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5196..7643
                     /locus_tag="SDA10_001853"
     CDS             5196..7643
                     /locus_tag="SDA10_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEG4240752.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7873..8940
                     /gene="rlmN"
                     /locus_tag="SDA10_001854"
     CDS             7873..8940
                     /gene="rlmN"
                     /locus_tag="SDA10_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEG4240753.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9133..10236)
                     /locus_tag="SDA10_001855"
     CDS             complement(9133..10236)
                     /locus_tag="SDA10_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG4240754.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3451 a         1793 c         2278 g         2936 t
ORIGIN      
        1 aatatggatt tggctaacta tggaagaata ctcgtattag ttttcagcgt tcatactagt
       61 tgttacattg tttgattggc caaaagaatt tctgatatat tttgtgctaa ataaagaaaa
      121 agacgcttga attgggaaat aagaaaacta ctctatcatt acaaattttc ataaactggc
      181 agatgttcta agtaaacaag tatcttttca aagtcggaaa aatccataat caacgtagtg
      241 gtattgatat ttggatgaaa gcctacggta tcttgacgat caatagcagc atcgatcacg
      301 acttggattt ttttctcttt gtcgtgcatc aatgctaatg gagtcactgt acctgctgga
      361 acgcctagga ggtcaggaag ctgttcttca gaaacaaaag ataagcgctt ttcttttaat
      421 tgttcagcta gttgttttaa atcggcgtgc ttttcatcag gcaagatgac aagatagatt
      481 tgttttcctt ttcttgattt caataataga ttttttactt gcggaccagg tagggaaaaa
      541 gggacatttt tgactgacgt aatcgcagga tgatcgactt tttgaaaaga gatacccagt
      601 ttttttagta gttcataagc ttcttgttcg gttgcacgac tcatcgcgca tcaccctcct
      661 ttttttatta gtgtatcata aaaaagagtt gacaaatgaa aaataatcat gtaaattaca
      721 cgtaaagtta aaacaaatga catcgaaaag aagagtagcc gttaggtgtt tttagagagt
      781 ctctggttgg tggaaagagg cagcagttaa ccgtgaaaca catcttggag tcgattttct
      841 gaagcgtata gtaggaaaat ccggggagcc cgttacagct gcggtcgttg ctagatgacc
      901 agataaggct ggcttttccg tgaggagtcg gtaaaaaaag gtggaaccac gtgattaacg
      961 tcctttgatc ttcggatcaa aggacttttt gttttcatct gaacggcaac gactgtgtga
     1021 ggttttttct gcaaggaaaa aataaacaga ggtggaacca cgtataatac gtcctcttgg
     1081 catttgccaa gaggattttt ttattttaaa ggaggaaaag aagatggagt atttgattga
     1141 agttatgccg caattattag taggagcact cacaacattg aaattatttt tatttacctt
     1201 gattggctca ttaccattag ggattgtttt agcattagga ttgtctttgc attttaagcc
     1261 gctacgctac ttgcttcaaa catatgtatg ggttatgcgg ggaacaccgc tgttgttgca
     1321 actgattttt attttttatg ggttgccgac gattgggatc gtttttgatc gtttcgaagc
     1381 agcactcatt gcctttatct taaactatgc agcttatttt gcagagatct ttcgtggagg
     1441 gatacaatcg attcctcaag gtcaatatga agcagcgcaa gttttgcaat taagtccctt
     1501 tcaaacggtg acgaaaatcg tcatcccaca agtgacaaag attgttttac cttctgtagg
     1561 gaatgaagtt atcaacttgg tcaaagattc ttccttgatc tatatattag gtttaggtga
     1621 cgtaatgcgt gctgggaaaa tcgcgatgga acgcgacgcg actttactgc ctttgctagg
     1681 cgtggcaatc atctatctta cattgactgg tattctaaca atcataatga aacaagttga
     1741 aggaaaatta aattattacc gctaactgat aggaggaaga aatatgattg aattgaagaa
     1801 agtgaacaaa gcatttggtg aaaaaaaaat aatcaaagat ttgaatctaa tcattccaga
     1861 tgggaaaaca ttagctatcg taggtccttc cggtggcggt aaaaccacgc ttttacgtat
     1921 cttggctggc ttagaaagtt ctgacagcgg agaattttat ttagacggtg taccgttcaa
     1981 cccattcgag cttaaaatga aagaacaagt tgtaggagtc gtattccaag acttccagct
     2041 atttccacat ctgaatattt ttgaaaacat taccttagca ccaaaaatgg tattgaaaca
     2101 gacaaaagaa gaatacacca aaaaagcaga agaattagcg gcttcattag ggattagtga
     2161 tttattgaat atctatcctt atcagctttc tggcggacaa aaacagcggg tagcgattgc
     2221 cagagcattg gctatgaatc cacgggtatt ggcttatgat gaaccaacaa gtgctttaga
     2281 tcctgaatta cgccagcaag tcgagcgttt gatcctatca ttgaaagaag atggggtaac
     2341 ccaaattatt gtcacccatg atttagtatt tgcagaaaat gtcggtgatc aattactaaa
     2401 agtttcccca attcattaag aaaggagaag atagagatga aaaaaaccag ttttctattc
     2461 gctttgatcg caggtcttct tctttttgca ggctgttcga acaaaaaaac ttcagcagat
     2521 aattgggcga ctatcaacga agagaaaaaa atcgttatag gtttagacga tacatttgtt
     2581 cctatgggat ttagagataa agacgggaag ctcacaggat ttgatattga tttagccaaa
     2641 gcagtctttg aacaatatgg gattacggta gactttcagc ccattgactg gtcaatgaag
     2701 gaatttgaac tgaacaatgg aacgatcgat ttgatatgga acggctattc caaaacgccg
     2761 gcaagagaaa agaaagtgca atttacaaaa ccttatatgg aaaacgatca agtgctgatc
     2821 acacctaaag aatcaggaat cacaaacttt ggacaaatga ataataagag attaggtgcg
     2881 caaaatgggt catctggtta tgatgtattt acgaaacaac cagaagtatt aaaagagata
     2941 gtcaaaaacc aagatgctgt tttgtatgat agttttaatg aagcattgat cgatctgaaa
     3001 agcagacggg tcgatggtct gctgatggat aaagtatacg cagactatta tttgaaacaa
     3061 agaaacgaac tagaagactt caacataaca aaaggttctt atgagagtga ggattttgca
     3121 gttggtgcac gcaagtcaga tagtacatta gtgaaaaaaa tcaatgaagc cctagatcaa
     3181 ctagagaaaa caggtgaatt ccaaaaaata tccgataaat ggtttggcga agatgttacg
     3241 ccgagaaatt aacaaatagg ggactgtgac aaaagacgtg tcacagtccc tttttccgaa
     3301 taaacagtgt tctcctgcca actccttgtg actcggagct gaatggcagg ctcacaacct
     3361 cttggtaaac ggtgttcaaa agccagtttt tcggtattaa gccaaattta tgcaaaaatg
     3421 tagaaagcca ttttcacaaa atttttctta ctactcgaaa ctaagcgact ttttcacaac
     3481 ctcctttaca taattttatt ttgttgtgga aggggtttcg tgttattcta aaagaggaac
     3541 gattgaaggt tataggaaag gaagagatga atggaacaca aagaacttac ctcatttcca
     3601 aaagattttc tgtggggctc tgcttctgca gcgtatcaag tagaaggagc ttggcaagaa
     3661 gatgggaaag gagaatcagt ctgggatcga tttgtacgga tacctggaaa gacatttaaa
     3721 ggaacgaacg gtgatttggc agtagaccac tatcatcgtt ataaagaaga tattgcttta
     3781 atgaaagaac aaggtctgaa agcttaccgc ttctctgtag cttggacacg gatttttcct
     3841 aatggacgag gagaagtcaa tcaagctggg ctcgcttttt atgagagatt gatcgacgaa
     3901 ttgattgaaa acgatattga acctatgttg actttatatc attgggatct tccacaagca
     3961 ttgcaagatg aatataatgg ttgggaatca cgtcagatca ttgaagattt cactcattat
     4021 gcagaaacat tatttgaggc attccgcggg aaagtccatt actggattag tttgaatgaa
     4081 caaaatatct tcacttcatt aggttatttg ttagcagcac atccgccagg tgtgacagac
     4141 ccgaaacgaa tgtatgaagt taaccatatt gctaatttag caaatgcatc tgtcatcaat
     4201 aaatttcacg agttagaaat gcctggaaaa atcggaccaa gctttgcgta tacgccaaat
     4261 tatccaattg acagtaatcc tgaaaatgtt ctggctgctg aaaatgcaga agacctgatg
     4321 gccaattatt ggatggatgt ctatatgtgg ggaaaatatc cgattgctgc catgaggttc
     4381 ttagaagaaa aaggatgggc accaacgatt gaagctggcg atgcagaatt gctagaatct
     4441 gctaaaccag attttcttgg gatcaattac tatcaaacgg cgacgaatgc attcaatcca
     4501 ttaaacggag ttggtgctgg aaaaatgaat acgacaggaa aaaaaggttc atcagaagaa
     4561 actggagttc ctggaatgta taaaaaagtt gagaatccat ttgtcgaacg aacaaattgg
     4621 gactgggaga ttgacccaga aggattaagg atcggactgc gtcgtatcac aagccgttac
     4681 cgcatgccgg tactgattac ggaaaatggc ttaggagagt atgacaagtt gacagaagat
     4741 aaacaaatcc atgacgacta tcgaatcaat tacttacaaa gccacgtcaa agccatcaaa
     4801 gaagcaattt cggatggagc agaagtccta ggctattgca cctggtctta tacagatttg
     4861 cttagctggc tgaacggtta tcaaaagcga tatggatttg tatatgttga tcaagacgaa
     4921 acacaagaag gatctttgga acgatataag aaagacagtt attactggta ccaaaaactg
     4981 attgaagaaa atggagagaa cctttagttc acatacttaa aacaagaagg tacggctgga
     5041 tatggccgtg ccttttcttg ataaaatcat ttagaaatta agaaaaactg taaaataagc
     5101 tgaaaaatga aagaaagcgt cagttctttc aaacaaaact ttagttccct tacttttttt
     5161 tgtataatga aacttgatga aatgagggat aataaatggc acaaaagaaa cggcctacta
     5221 aaaaaaggaa aaagacgaaa aaaacgaaaa agcaaaaaca gcagcaggaa caaatgctga
     5281 cgattggtgt tgggtttgcc ttgattttat ttgctgtctt cggcttttta aaattaggtt
     5341 ttttaggaat attgattgca aacggctttc ggatcattgc aggaaatact taccaaatcc
     5401 tctgtctttt attagccgta ttaggatttt ggattgtcat taagaataca gagttttcaa
     5461 ttggtaaaaa ccgacgctgg tttggcggca tactctttta tttcggtatc ttattattgt
     5521 tacatgccca tttatttggc aaactccata ctggggaacc aaatatcatg gggacgacat
     5581 gggatttgtt agccagtgat ataaaacaaa gtcaagtaga caataacgtt ggtggtggga
     5641 tgattggagc aatcctttat cattttactt acttcttgat tgcacagcct ggaagttatt
     5701 tagcagcagt gctattactg gcaggaggag cattcctgtt cagtaatctg gaaggctatc
     5761 agctgttaaa tggtatccaa tcaattggag aacgagtaca ggaattattg gaaggtgatc
     5821 ctgccaagca agctcgaaag caagcggcaa aggaagagag aatgaaacag cgcgcagaag
     5881 cgaaagaagc aaggcgctta gctgcaaaag aagctgcaga aaaagaagct gcagaatatg
     5941 agaagaaaaa agcggtgagc cagcaaaggg aaaatactgc tgatgaatgg caggaaccag
     6001 aaaatacaga gccggagcag ctaagttttg ttccaatcga cagttttcaa gaaaatatcc
     6061 atcctgcgaa tctagagaaa ccagtaccag atacaccaaa acagaccaat acagcagaag
     6121 gtttttcaga tgagttgcca gaggatgacg gtacctcatt agaatttgag attgaagcag
     6181 aacaggaaaa tcaagactat gaattaccga cagttgattt gcttgattct ataccaactg
     6241 tggatcaaag tgatgagtat aaaaaaattg aaaaaaatat cggcgtatta gaacaaactt
     6301 tccaaagttt cggtgtcgat gccaaggtcg tcaaagccag cttaggacca tccgtcacaa
     6361 aattcgaagt ccagccggct gtaggggtaa aagtaagtaa aattgtgaac ttaacagatg
     6421 atatcgcgct tgctttagcg gcgaaagatg tccggatgga agcaccgatt ccaggtaaat
     6481 cactgattgg tattgaagta ccaaatggaa agatcagtat ggtttccttt agagaaatca
     6541 tcgaagcaca accaaaccac ccagataaat tactagaagt acctttagga cgtgatgttt
     6601 ctggacgggt acaaacggct gatctttcga agatgcctca cttactcgtg gctggttcga
     6661 ctggtagtgg gaagtcagta gcaattaatg ggattatcac aagtatatta atgcgtgcta
     6721 agccccatga agtaaaactg atgatgatcg atccgaaaat ggttgaattg aatatgtaca
     6781 acggcatacc tcatctgttg acgccggtcg tgacgaaccc tagaaaagca gcgcaagcct
     6841 tacaaaaagt agtacaagaa atggaagaac gatatgaaaa attcgcggca accggagtaa
     6901 gaaatatttc tggttacaat gaatttgttc aacagaaaaa cttggaaaac ggaacgaaac
     6961 atccaacctt accattcatc gtagtgatcg tagatgaatt agctgatttg atgatggtag
     7021 ccagcaatga agttgaagat gcgattattc gtttagcgca aatggctcgg gcagcgggga
     7081 ttcatatgat cctagctact caacgtccaa gtgtggatgt tatcacagga atcatcaaag
     7141 caaatgtacc ttccagaatg gcatttgcgg tatcaagcgg tacagattct cggacgatca
     7201 ttgattctaa tggggcagaa aagctcttag ggcgaggaga tatgctattc ttgccaatgg
     7261 gtgagaataa accgatccgt gtgcaaggag cattcatttc tgaccacgaa gtagaacggg
     7321 tcgttcaatt tgttacagat cagcaagaag ctcattatga agagaaaatg atgccgacag
     7381 atgaagttga aacgacaggt gctccagaac agccacaaga tgagctgttt gaagaagcaa
     7441 aagcacttgt cgttgaaatg caaacggcaa gtatttccct tctccaaaga agatttcgga
     7501 tcggttacaa ccgagcagca cgtttagtag atgaactgga agctcatgga gttgttggcc
     7561 cgtcagaagg aagcaagcct cggaaagtat tcattgagca ggaagaaaca tcagaaacgt
     7621 tgccggacga taatgacatg taaaacttgt gtgatgaagg gttgaaaaac gaataaccaa
     7681 taaaaagaca aggcatgatg ataccaaaag aaaaaatggt ttttttccat cttttctaat
     7741 acggtatgat gtcttgtctt tttagttttt gtgagattaa agttctattc gatttatttg
     7801 tgaagtattg cattaaggaa atgaatcatg tatagtatgt agcgaaactt tgagaaaaag
     7861 agggaaaata ctttggaaaa acagtccatt tatggcttga cgaatgaaga attaatcaat
     7921 tggtttatag aaaacggcga gaaaaaattc cgagctgccc aagtttggga atggttgtat
     7981 caaaaacgtg taagtaattt cacagaaatg acgaatctat cgaaacaatt gatcgaaaaa
     8041 ttatcggagc attttatcat caatcctcta aaacagatgg tcgtccaaga agcttcggat
     8101 ggtacagtaa aatatttgtt tgaactccct gataaaaaca tgattgagac tgtactgatg
     8161 agacaagaat atggcttgtc agtttgtgtg acgacacaag taggatgtaa tatcggctgt
     8221 acgttttgtg caagcgggct gctcaaaaag aaccgtgatc tgactgctgg agaaattgtt
     8281 gcacagatca tgatggtcca acattatttt gatgagcgca atcttggcga acgcgtttca
     8341 catgtcgttg taatggggat cggtgagcca tttgataatt acgacaatgt aatggacttt
     8401 ttgcatatca tcaatgatgc caaaggattg gcaattggtg ctcggcacat tacagtttcc
     8461 actagtggat tagcacacaa aatcaaggaa ttcgctaata acggcttgca agtcaatctg
     8521 gctatttcct tgcacgcgcc gaataatgag gtgcgaacat cgatcatgcg gatcaaccga
     8581 agtttcccaa ttgagaaatt gatggaagct gtggatgagt atttagagaa aacaaaccgt
     8641 cgaatcacgt ttgaatatat catgttgaat caagtaaatg atagaccaga gcatgcgcaa
     8701 caattagcag atctattaaa agataagaaa aaattagcat atgtcaactt gattccatat
     8761 aatccagtca gtgaacatga tcaatattct cgcagtccaa aagctgatgt attgagattt
     8821 tatgatgtat tgaagaaaaa cggcgtaaac tgtgtgatac gtaaagaaca tggtacagat
     8881 attgatgcag cttgcggaca attgcgaagc aagcaaatga aaaaaacagt tgccaaataa
     8941 tcgtatccta ctcgtttttt caattaccat aaaaggagca gttttcttaa ttaaggaact
     9001 gttcaactta aataagagaa aagattctat agcgaaagct ataaagcttt ttttgacaag
     9061 aaaaaccctc atcaagcaaa gaaatgtcct caatttgatg acagcctgct tgatgggggt
     9121 tttagcaatt atttaactga ttttcttata tccttgctgg ctttcttgtg tagcatgata
     9181 agtttctacg tcaagaatgg cctgtttaaa tttttctcgg gtgatttcat aaggcacatg
     9241 tttaatatca tttgttgcca tggctttatc taagattgct tctaaatcgg tcgtgacatt
     9301 gagattcatg tcggttaacg atactggaag ttggttcttt ttcataaaca catagatttt
     9361 atcaaacaaa tcgaactgct gatccatcat caataaggtc agtacaccat aactgacaga
     9421 tgctccgtga aggtatccct ccattttttc tagaacagtc gtaccgtaat agaatgagtg
     9481 tgccatagca gaattatagt tattgttaac taaaacagaa accaatccgg tagtaacaat
     9541 gatatccgaa acgacttgtt caattgcata tgagggacga tcgtcggcac tatctttcaa
     9601 tgcttgcgta ccataagtca agagacgatc aatgcacatt tttccgattt ggattcctaa
     9661 tctatccgtg tagcctagat catcgccacg agcagaaaaa gtagattcga cttctttgga
     9721 taaagcatcg ccaatacctg cccataagta tttatcaggt gcttcggcaa taattgctgt
     9781 atcaatgaag gtgtgtacag gtggttttga aggaaagaag agtccgtcca ttacgccttc
     9841 ttcgttatag atgacacata ccgcagtgac gggcgaacag ttcgaaccaa tagtgggaaa
     9901 ggtgaagact ggtttagcta gtttttcacc aatgactttt acagtatcgc aagcacggcc
     9961 accaccgact gcaaacagga cgtctgcttt ttggacactt tcatgctgga tcaactgttc
    10021 aatatttcca taagttgaat tacccccgta ccagagactg gcgacaacct ctatttcctg
    10081 tgtcaaagct acttttattt tgttttctgc ctttgaaagt gcagtcttcc ctccaatcag
    10141 cactactttt gtcccatatt ctttgacgat tccatttgct ttttcatacg catctgttcc
    10201 aatggtataa tttggtaaga aaacactttg actcatattc atttccttct ttcttcgttg
    10261 aaacgggtta aaattttaaa gagtgatcga tctgactgcg gctgtagatg gagtagaggt
    10321 gaacctaaga caggataagt gatgaataag gtggcatgaa caccttgaaa tcatcaataa
    10381 gtatcctaaa aacgaacaag tacaaaaaca ttgaaaacga caataatgaa tttttgttcc
    10441 tcttagtatt tctaccga
//
LOCUS       DARDHA010000091        10262 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000091 DARDHA010000000
VERSION     DARDHA010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.092"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(243..1091)
                     /locus_tag="SDA10_001856"
     CDS             complement(243..1091)
                     /locus_tag="SDA10_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG4240755.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1090..2157
                     /gene="gnd"
                     /locus_tag="SDA10_001857"
     CDS             1090..2157
                     /gene="gnd"
                     /locus_tag="SDA10_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEG4240756.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2159..3160
                     /locus_tag="SDA10_001858"
     CDS             2159..3160
                     /locus_tag="SDA10_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEG4240757.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3157..3441
                     /locus_tag="SDA10_001859"
     CDS             3157..3441
                     /locus_tag="SDA10_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEG4240758.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3474..4271
                     /locus_tag="SDA10_001860"
     CDS             3474..4271
                     /locus_tag="SDA10_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG4240759.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4285..5118
                     /locus_tag="SDA10_001861"
     CDS             4285..5118
                     /locus_tag="SDA10_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4240760.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGGFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5136..5993
                     /locus_tag="SDA10_001862"
     CDS             5136..5993
                     /locus_tag="SDA10_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG4240761.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6015..6524
                     /locus_tag="SDA10_001863"
     CDS             6015..6524
                     /locus_tag="SDA10_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4240762.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6561..6962
                     /locus_tag="SDA10_001864"
     CDS             6561..6962
                     /locus_tag="SDA10_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240763.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7074..7427
                     /locus_tag="SDA10_001865"
     CDS             7074..7427
                     /locus_tag="SDA10_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240764.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7461..7904
                     /locus_tag="SDA10_001866"
     CDS             7461..7904
                     /locus_tag="SDA10_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG4240765.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7921..9006
                     /gene="uxuA"
                     /locus_tag="SDA10_001867"
     CDS             7921..9006
                     /gene="uxuA"
                     /locus_tag="SDA10_001867"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG4240766.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8999..9712
                     /locus_tag="SDA10_001868"
     CDS             8999..9712
                     /locus_tag="SDA10_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG4240767.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9919..10068)
                     /locus_tag="SDA10_001869"
     CDS             complement(9919..10068)
                     /locus_tag="SDA10_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240768.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
BASE COUNT         3422 a         1629 c         2236 g         2975 t
ORIGIN      
        1 acaaaaataa taaaaaaaag aaaagataaa ttttttacta ctgtatacta actttgttat
       61 cgcatctaac aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat
      121 acgttctaac ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac
      181 taccaaatat ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg
      241 tgctattctt tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct
      301 ttttcgttgt aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata
      361 cgggaagcta aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca
      421 tcgccaagtt tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca
      481 gtttttctta cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc
      541 aaaacagcac agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc
      601 gtgctgtgag aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca
      661 atctctgaac cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc
      721 gcttttttta gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta
      781 gactcgaaca ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg
      841 atagaaatag cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat
      901 ccattataac caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca
      961 cttgctaaat tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg
     1021 gcgatagcct gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg
     1081 gagacgctca tgagaattcc tcattttcat aataagatta tcattttttc cgttctcatt
     1141 aatgttaaag atacaaggag agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct
     1201 atttacaaaa aaattttttt gtataatgag ggtataagaa atggaggcgc tatcatggaa
     1261 attggtgtaa ttggtttggg aaaaatggga ttgaatttag ctttaaactt aaaagagcac
     1321 catcacaaag tacaaggact cgacatttca agtacagctg ttagtcaggc aagagaaaat
     1381 gacattcctg cttatcaaga gatctctgaa tttctagcta cttttgagca gaaaaaaatc
     1441 atctggttaa tgcttcctgc tggagagata accgaaaaca tgttaagaaa actttttcca
     1501 aaactcggac caggagacat tattattgaa ggcggaaact cttattataa agattcaatc
     1561 cgtcgagcag aagagtttca gaaaaatgaa atcggttact tggattgtgg gacttctgga
     1621 ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg gaggagataa agaaactttt
     1681 ctatccgtgg aacaattatt caaagatgta gcaattgaaa gaggatatct gtatgctggt
     1741 gcatccggaa gcggacactt cttgaaaatg atccataatg gaatcgaata tggaatgatg
     1801 caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt tgacttggaa
     1861 caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg gctggctgat ggaaatcatg
     1921 gaagatcagt taaacgaaca tcccaattta agtaattatc ggggaatcgt ttctgcatca
     1981 ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt
     2041 gcaactagtt tgttcatgag gaatctttca caggaagaag atagtttttc agcaaaagta
     2101 gtttcagcac taagaaatgg gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat
     2161 gccagattat catatagcaa tcgttaattc cagcagtttc ggaagagtct ttccacacca
     2221 cttggaaaga ttaaagtcag ttggagacgt tcatttcttt actgttgatc aagagattcc
     2281 aggaaaagaa ctagcggagt tacttaaagg attcaatatc attattgcaa gtgttacgcc
     2341 attttttcga cgagattttt ttgaaaacaa agatgaactg ctattgattt cccgacatgg
     2401 tattggttac aacaatattg atttaaaagc agcaaaagaa caccagacaa tcgtatctat
     2461 catacctgct ttagttgaac gaaatgctgt agcagaaaat aatataacta atttgttgtc
     2521 catgcttcga cgcacgacac aagcacaagc aagagtcaag gacgatcgat gggaagatcg
     2581 ggcggagttc gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa
     2641 taccggaagc ggagtaatcg aaattttgcg taacggtttt cgctgtgatg ttctagctta
     2701 tgatccttac aaatcagatt tatacatcga aagctttggt gcaaaaaaag tagaacttca
     2761 agaattactg gaacaatcag atatcatttg tttatgtgcg aatttgacag aagagaacta
     2821 tcacatgatt tctgataaag aagtcgatca gatgaaagaa aatgtttatc tatctaatag
     2881 tgcaagaggc gctctagttg atgaagaagc gatgattaat ggtctgaaaa gcggaaagat
     2941 cgcaggatat gccacagatg ttttagaaga agaaccggga cgtcaagatc atccgtattt
     3001 agcgtttgaa caagtagtga ttacgccaca tatatcggcc tacacaaggg aatgtttaga
     3061 agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc ttcttcctga
     3121 acgttctgtc cagacaaaga gcaactatat aaaaggatga tgaagaaaat ggtgaacggt
     3181 ttaaaagtga aaacaggccc tcaattttat ttatatgaag aaggtggtat ttcaaaagtt
     3241 tctgatctct tgaaatcata cggcgcgaaa agagtactgg tcacccacgg aacggtatca
     3301 tgggaaaagg cgctacctaa attagtattt ttgaatgacg aaacgattca atttttttat
     3361 catcgttatt ctggagaatg tagttatgct gaagccagac gaatagctac aatcatcaaa
     3421 aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa actgaccgat ctagtgggtt
     3481 atagtgccaa tcttgcaaga atcgatttcg gagtcatccc tactttagct agtaattgtg
     3541 ctccatggac acctcttgct gtcatgtata aggagaatgg acaagcagaa gggaaaacag
     3601 aacactttta cagacaagct gcttttttta taacagatcc ccaattagtg attgattccc
     3661 ctgttccata tttcattgcc gggttagcag atacactagc aaaatggtat gaatctgagc
     3721 tcatcttaga acaagatttt ttgcaagatg aatcattttt gaagctagca caagatactg
     3781 cgaagatctg caaagaagag attttgaatc attctgctaa agcaatccaa gatatggcac
     3841 aaagaaaact gacatcggag ttcaaacagc tttcagagat tatctttgct gttgctggtt
     3901 tagttggagg attaggagat aaatacgcaa gaaatgcggc agcacatgct atgcatgacg
     3961 ctatcagtaa atatattccg gaaagccatc gttttctaca tggagaaaaa gtagcgtatg
     4021 ggattttcta tcaactggca ctagaaggtc gctgggaaaa aatagacgag ttatatcctt
     4081 tgtatcggga attagaactg ccggtttctc tagaacaaat gggattattt ccgaaagata
     4141 aacagataat agataaaatg gctgaattta ttgattcgaa agagaaagtg catttgattc
     4201 ctatagaggt aacacagaaa tcactgatac agtcaatgat ggaactggaa ggttatatca
     4261 gaaagatctg aaggaggagg acacatggaa acattaagtg tagtacaaag tttgttgatt
     4321 gctttatggg tagccgcggt tatgtcccgc tggttaggtg gaggagccac gttgacctta
     4381 cgtttttctc ctttgatgac tggattagtc gtcgggttga tcatggggga agttgcacaa
     4441 gcgatgattg ttacagccgc tttgcaaatg atttatatgg ggggtttttc tccaggagga
     4501 tcaatgccag ctgaaccatc tattgcagca gctatcgcag taccagtcgc attattaggg
     4561 gatctgtcgc cagaagcagc gattgccgtt gcggtgccag tcggattgct cggaagttac
     4621 ttgtatcagt ttcgtttttt catcaatact tttttaggta aatatacaga caaagctgta
     4681 gaagatttga atacaaaagc tatggcccga tcaattattc tttatccaac ggttgcctca
     4741 tttttattat tcgttccgct tgttttcatt gctttatatt taggggcgcc attgatcgct
     4801 gatgtaatca aagctttaga gggaacagtc gttatccaca ttcttgaagt agtaggtgga
     4861 gggttagcgg caatcggtat cgcgacaact gtatacgtta tcggtcgaaa agattatctg
     4921 gtctttttct tccttgctta ttttatgagt gttgttttga aatctttaga tattacgatg
     4981 gttacttatg ccatttttgg gattattatc gctttgatct ttgtccagtc gcaaaaaggg
     5041 aaacaagtac agcctgccac aagcaataac aattcagcat cttttgatga cgaagatgac
     5101 tatgatgacg gattttaatt taggaggaat gggaaatggc tgaaataatg gataatcaaa
     5161 caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg agatggcatt
     5221 ttgcaaacga aattcctcac tcttttgaac gttacttggc gccatcacta ctatacggaa
     5281 tgatgccaat cttgaaaaaa ctttataaag atgaagactc tttaaaagca gcatataaac
     5341 ggcagttact gttcttcaac actcaattaa gctggggtgg cggtgtgatt actggcctaa
     5401 tgtcttctat ggaacagcaa agagcagaag aagagtatga agaaaaagag atcttgatgc
     5461 aggatgactt gatgtataac acgaaagcgg gattgatggg ggcactagca ggaatcggag
     5521 atgcaatcga ttcaggaacc gtccaatata tttttatcgc aatcgcggta ccttgggcac
     5581 aacagggaag tcctttaggc gcactttttc cttttatctg ctttgggttg tatcaggttt
     5641 tactaggggt atttttcgca agacaagctt tcactatggg ccgtaatgct acaggcctaa
     5701 tgcaaaacac tggtgttcag acagcgattg aaatgctatc agttttaggg ttgtttatga
     5761 tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc attgaaattt gcgatatccg
     5821 gacgtgagtt tgttatccag gaaattcttg atcaaatcgt gccagggata ttacctttag
     5881 ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc acacaagcat
     5941 tattatggct gactggaata ttaattgtgc tagcaggaat cggtatttta taaaaagaag
     6001 ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg
     6061 ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg
     6121 aagttgtagc aaaagataaa tttatgagag atctttacaa aaatgcagga agccgtacag
     6181 gacaaaaaac aattgtcatt acaccagaga aagctaaatt ctattgggat gaatatcaat
     6241 ttaaagaata caattgtatt ttgatcgcaa aaacagtcca aacgatttac gacttagtaa
     6301 aacacggaat ccccatggaa gaattgaata ttggagggat tgctcaaaaa aatcctgata
     6361 cagatctttt agtgacgaaa tctgtttatt taaataaatc agatgctgaa aaattgaaag
     6421 aaatgaatga acaatatggc gtaaagaata tttattttca agccactcca tctgcaccta
     6481 aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa
     6541 aagataaaga gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt
     6601 atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat
     6661 tagtatcaga tatttacagt atgagaacag atgtgacagt cattgcttac gatacgctag
     6721 cctaccgatc aaaaaatatt tatgttggag atatcgaaaa agcagagaaa gttatctgta
     6781 cttattacga tacacccgtt catgcattag gaagttattt catgtttgat tggaaagacc
     6841 aaaggaaaaa aacaatctac tctatattat tgtccttcat tttgttgttc agtttaggtt
     6901 ggtggggaat gatgatatat aacaaaaatc ctcatcacgt attcgatctg ctatccgtat
     6961 aaacaggtat cactgtacta gcatttggga gctacttttt cttattggga aaagcagctc
     7021 gcggatggcc tagcagacaa acttttattc gaaatacttc atccatatta acgatgctag
     7081 aaatgatccg tacgattgat gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt
     7141 atggagaaaa gggcttagac agcgtgagat cgggtatgaa aaaagaggct attctttttt
     7201 atctagactc tgttggggca gatacaccgc tacagttttc gggaaattat ttttcaaata
     7261 aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat tatattttta
     7321 gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa aacagattta cgagggaaaa
     7381 catttaattg gcaaaatgcc aatcagatta ttgctctttt tcgataagta aaaagtgcaa
     7441 aacaaggagt gagaagacta atgttggaaa tcatgatcgt tacacatggt aagttgagcg
     7501 aaggactgaa agatgcggcg gaagtaattg tcgggacatc gagccatatt gaaacaatgt
     7561 gcttagagtc tggggaggac atccaaacat tcggagagaa ggtcaaagaa aaattaacta
     7621 tgatcaacac agcagaaccc atgctagtat ttgttgatct aattagcgca agtccgtaca
     7681 atcaagcgct cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc acttatgtca
     7741 tagggggcgt gaatctgcca atgttgttag aagcaatcaa tcatcagatg cttcacacac
     7801 cgatagaaga agcggtggaa caaattgtca aacaaggaaa agataattta actgtttggc
     7861 atgcttcagc ggaagaaaaa aaattagatg aggacgattt ttagaaggaa ggctgaaaag
     7921 atgaagtggg gatttagatg gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt
     7981 cgccaaatac cgggaataaa tgggataatt ggaactttgc tgactaaaat gcctggagat
     8041 gtttgggaga tttcagaaat tgaggagttg aaagcatctg ttgagagcca aggactggaa
     8101 ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca aggcagggac agatgaacga
     8161 gattactata ttgataacta tatacgaaca atcaaaaact tgtctcgatg tggggttcga
     8221 ttgatttgtt atagcttcaa accaattttt ggatgggcga aaactgattt gtttaatgaa
     8281 aacaacgatg gaagctattc tcttttatac gatcagaaaa tcgtcgatca gatggagcca
     8341 ggagaaatgt atcaattgat acacagccag tcaaagggat tcaaattgcc gggttgggaa
     8401 gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa
     8461 gaattatttg aaaatctgac ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca
     8521 gatgtaaaaa tggctatcca tccagatgat ccgccatggg aactttttgg ttttcctaga
     8581 gtgacgaaaa atcttggcga cttgaagaaa attctaaatg cagtagattc tccatataat
     8641 ggactaacgc tgtgcacagg atctttaggg gcaaatccgg aaaacgattt agttgaaatc
     8701 atccatgagg taggttcgcg catccatttt gtccacttca gaaacgtgga gtttttagga
     8761 gaaagaaagt tcaaagaaac ggcacatccc tctacagaag gctcccttga tatgtatcat
     8821 attatgaaag cgttggtgca agaagggttc gacggtccta tacgccctga tcatggtaga
     8881 accgtgtggg gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc aatgggactc
     8941 acttacatgc aaggtctata tgaggcgata agtaaggaaa aaccactggg agagcattat
     9001 gaataaaatc aagctcttca tctttgatat ggatggcctt ttattagaaa ctggacgttt
     9061 agcttatcgt gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt
     9121 ctattattta ctaactggcc aaacagaaat ggcgataaga gaacaaatgg gcattttgta
     9181 cggagaagat gttccatata tcaaatggcg agaagcaatc aatcaatata aagaaaaaat
     9241 cgtgaaagaa gataagagag tatatacgaa aaaaggagca gaagaaattt tatcttttgc
     9301 aaaagaaaga ggaatccata caatcgtggc ttcgtctaat acacgggtaa aagtagaaat
     9361 gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa atcatttcag gggatgatgt
     9421 aaaaaaagga aagccggaac cagagatctt tttgaaagct tgttcaaaaa tgaatattcc
     9481 tccaagtgaa gcacttgtat ttgaagactc gattgcaggg atcgaagcag caaggcgagc
     9541 gggcattttg tcttttctca tcaaagatca tttgataggg ttgcccgatc ataatgggaa
     9601 gcataagttg aaagtagatg taagtttata tgaatccaat aaagcagact atgaatttta
     9661 tgacctgcat caagcaaaac gatttttaga aagtaaaggg ttgtatctct aataaagagg
     9721 actattttct agtcctcttt atttattgtc ctgcacgact gagggaacag atgaaaaaga
     9781 agtaattttt tattttgaag tatctacctt atttgtagtg aacaatttag aacaaacaca
     9841 aaacgcatct actgttatct accagtagat gcgtttaaag agaagaacga gaatcgtcca
     9901 tatgagggtt tccactcctt atgcagattt ttttaattct tgcactgctt gttcgatcgt
     9961 aacacctgtc gctacatgat tggtattttc tttgtcaaaa acgacaaata gattcaaatc
    10021 tgtatccaag gttactcgat attgtatatc tttgttatag aatgtcatag ccattctcct
    10081 ttctttaaat acttgctgaa aggcaacgaa cgcgttgata acgaaattcc atttataagt
    10141 ataccaccaa ttgaaaggtt ttcatagtta agaaatttta aagtcaaaag ttgcagataa
    10201 aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg ttattcaact ttttcttcga
    10261 tt
//
LOCUS       DARDHA010000092        10192 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000092 DARDHA010000000
VERSION     DARDHA010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.093"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            119..625
                     /locus_tag="SDA10_001870"
     CDS             119..625
                     /locus_tag="SDA10_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEG4240769.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            642..1541
                     /locus_tag="SDA10_001871"
     CDS             642..1541
                     /locus_tag="SDA10_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEG4240770.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1628..2512
                     /locus_tag="SDA10_001872"
     CDS             1628..2512
                     /locus_tag="SDA10_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEG4240771.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2704..2775)
                     /locus_tag="SDA10_001873"
     tRNA            complement(2704..2775)
                     /locus_tag="SDA10_001873"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2740..2742),aa:Arg,seq:ccg)
     gene            2835..4169
                     /gene="rpoN"
                     /locus_tag="SDA10_001874"
     CDS             2835..4169
                     /gene="rpoN"
                     /locus_tag="SDA10_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEG4240772.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4318..5409
                     /locus_tag="SDA10_001875"
     CDS             4318..5409
                     /locus_tag="SDA10_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HEG4240773.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5450..6451
                     /gene="gap"
                     /locus_tag="SDA10_001876"
     CDS             5450..6451
                     /gene="gap"
                     /locus_tag="SDA10_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEG4240774.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6561..7751
                     /locus_tag="SDA10_001877"
     CDS             6561..7751
                     /locus_tag="SDA10_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEG4240775.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7855..8610
                     /locus_tag="SDA10_001878"
     CDS             7855..8610
                     /locus_tag="SDA10_001878"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEG4240776.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8726..10024
                     /gene="eno"
                     /locus_tag="SDA10_001879"
     CDS             8726..10024
                     /gene="eno"
                     /locus_tag="SDA10_001879"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEG4240777.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3565 a         1886 c         2112 g         2629 t
ORIGIN      
        1 tttgtcacac cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa
       61 attgaaactt ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat
      121 gatttttact ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg
      181 actcatcgaa actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa
      241 agaagaggcc ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa
      301 agaaaaagag atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt
      361 agatagttgg cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc
      421 ccaacagtta cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga
      481 cagtccggaa aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat
      541 tgcgactatc ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa
      601 tgctttacgt caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa
      661 atttaaaaaa actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg
      721 agatccgcca gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga
      781 gacgtctcca agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc
      841 tttttaatag tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg
      901 aattgatctt aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga
      961 agatcaatac acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact
     1021 ttcgcgaagg acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg
     1081 atcattacca agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga
     1141 ttgaaaggca gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa
     1201 tcgacaagaa aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc
     1261 tatctgtttt gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat
     1321 ctaagtacta ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg
     1381 ttacgcctac acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc
     1441 aatacgaaat tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga
     1501 caaaaacgat tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact
     1561 agcagtttca attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag
     1621 agatatagtg aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt
     1681 tttcgaaatt acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc
     1741 ggagcattcg tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac
     1801 tgaaacagct aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc
     1861 atcacaatca acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc
     1921 aatcagcttt gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa
     1981 agaaaaaaac gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga
     2041 aatcgtgaag cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca
     2101 gaatctggcc actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga
     2161 gatcaaaaaa gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac
     2221 gaatgtagga gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga
     2281 ttggaagaca agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg
     2341 ggcaattatt ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat
     2401 cgattatctg aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc
     2461 aacggctgtc gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca
     2521 agtatcttta ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc
     2581 aaaggcttaa caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg
     2641 gacagttttc acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta
     2701 gaacgcgtcc tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca
     2761 ctacactaag gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg
     2821 gattggacaa aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga
     2881 aactagccat gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg
     2941 agctgttggc attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc
     3001 cggaatggca gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa
     3061 attacctaaa tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag
     3121 tccatttgaa ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca
     3181 tcgatttgaa cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta
     3241 cgcctatcca aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag
     3301 gtgcacgtag tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg
     3361 aattagctta tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag
     3421 aaatcgcaca acgtttttct gttgatctac atgctgttca gcagatattc gactatttac
     3481 aaacgttgag tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc
     3541 ctgatgtccg ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa
     3601 atcagccaaa tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata
     3661 aagaaactga aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga
     3721 tcctacaacg tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag
     3781 ctttctttat cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg
     3841 agatagatgt gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag
     3901 acttcggtgt atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag
     3961 aacagacaga agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc
     4021 aagaagacaa aaacaagccg ttgtctgacc agaaattggt tgaattgctg aaaaaagatg
     4081 agatagcgat ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt
     4141 catcaaaaag aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact
     4201 ttttctagtc tttttagttg aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt
     4261 tgagatttcc ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg
     4321 ggtcgtttta agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat
     4381 gaaataaaaa tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat
     4441 aaaattttac gaaatatcta ttggatgcag ccgattggac gtcgaagttt atcggaaagt
     4501 atggggatca cagaacgagt attgcgaaca gaaacagatc ttttgaaaaa tctgaatttg
     4561 atcgacacct caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc
     4621 cttgaaaact tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa
     4681 tattttggta accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg
     4741 gctgatgcat tcggcgaagc gctgagcgaa gccctagacc gactacttcc agaaggcgag
     4801 aatatcatcg ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat
     4861 ctggaaaata agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg
     4921 ataactgtac aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac
     4981 cgtgcacttt atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag
     5041 ccatctatcc aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt
     5101 gggcgtgcat tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg
     5161 aaacaagcaa atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc
     5221 gtttataagg tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc
     5281 atggcaatcg cgggcggaaa atcaaaagcc aaagcaattc gagcttatat gaaaaatgca
     5341 ccaaaacaaa cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta
     5401 accctttaaa ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa
     5461 agtaggtatt aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt
     5521 agatggaatc gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt
     5581 gttaaaatac gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc
     5641 attcaacgta aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc
     5701 atggggcgaa ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc
     5761 agcagctgaa aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg
     5821 aaacgatgtt ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac
     5881 agttatctca ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa
     5941 cgacaaattt ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca
     6001 aatgactcta gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc
     6061 aaacatcgtt cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcctgaatt
     6121 gaacggtaaa ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga
     6181 attagtaaca gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga
     6241 agcttcaaac gaatcttacg gatacaacac agacgaaatc gtttcttcag atatcgttgg
     6301 tatgacttac ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa
     6361 acaattagtt aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt
     6421 acgtacttta gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata
     6481 gacatgtaca tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag
     6541 tttattcagg aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca
     6601 aaaaagttct tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg
     6661 acactcgtat caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag
     6721 caatcctttt ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt
     6781 tagctccagt tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg
     6841 aaactcgcgg agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg
     6901 tattcgaaaa tacacgattt gaagatattg acggtaaaaa agaaagcaaa aatgatacag
     6961 aactaggtaa atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag
     7021 ctcaccgtgc acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg
     7081 gattcttgat ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgac
     7141 caatgattgc aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact
     7201 tgattcctaa agcagataaa atcttgatcg gtggcggaat gacttacaca ttctacgaag
     7261 caaaaggaat caagatcggt aactctctag tagaagctga taaagtggaa ctagcaaaag
     7321 aattaatcga aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag
     7381 aattttcaaa tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg
     7441 ctttagacat cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa
     7501 cagttgtttg gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa
     7561 tcggtgtttg tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag
     7621 actcagccgc agcagcagaa caattaggat ttgctgacaa attcacacac atctcaacag
     7681 gtggcggcgc aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca
     7741 acgataaata aacgaccaag tttgctcaat tctgacgagt aatcgatcaa aaggtgaagg
     7801 gagttttcat gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt
     7861 aaacctatta tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt
     7921 gctgaagcag taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct
     7981 cctgcattat tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca
     8041 gcacaaaact gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct
     8101 cttgctgatc taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc
     8161 catgaaacag acgaagacat caataaaaaa gcgaaagcta tctttgcaaa tggtatgaca
     8221 ccaatcctat gctgtggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg
     8281 atcgaaggac aaattacagc tggtttagct gatctttcag ctgaacaagt gtcaaacatg
     8341 gttattgctt atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc
     8401 gctgacgaaa tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta
     8461 tcagaagcag ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac
     8521 atggcaaaag aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca
     8581 ttcttgtcat tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa
     8641 tgtttgcact aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa
     8701 aacaaactca aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat
     8761 cttagactca cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt
     8821 tggccgcgga atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg
     8881 tgacggagac aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa
     8941 caacatcatt gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa
     9001 agctatgatc gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt
     9061 aggtgtttct atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca
     9121 ctacctaggc ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg
     9181 cggatcacat gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc
     9241 tccaacattc aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat
     9301 cttgaaagct cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct
     9361 tggttcaaac gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt
     9421 acctggtaaa gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga
     9481 aaaaggtgtt tacgtactag ctgattcagg cgaaggcgaa aaaacaactg acgaaatgat
     9541 caaattctac gaagaattag tttctaaata tccaatcatc tcaatcgaag acggattaga
     9601 cgaaaacgac tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt
     9661 agttggtgac gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg
     9721 aattgctaac tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga
     9781 agctatcgaa atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga
     9841 aacagaagat tcaacaatct ctgatatcgc tgttgcaaca aacgctggcc aaatcaaaac
     9901 tggttctcta tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga
     9961 ccaacttggt gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa
    10021 ataattcttt tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac
    10081 ttagttaagt aatcgaactc tctacttgct gaaatatcgg ctctttgtca ataaggactg
    10141 atgagttgtg caaaatcaaa tctgagtcag aatgaaccac attctggctc ag
//
LOCUS       DARDHA010000093        10177 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000093 DARDHA010000000
VERSION     DARDHA010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.094"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..808)
                     /locus_tag="SDA10_001880"
     CDS             complement(<1..808)
                     /locus_tag="SDA10_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4240778.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYY"
     gene            complement(937..1584)
                     /locus_tag="SDA10_001881"
     CDS             complement(937..1584)
                     /locus_tag="SDA10_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEG4240779.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            complement(1668..3095)
                     /gene="uxaC"
                     /locus_tag="SDA10_001882"
     CDS             complement(1668..3095)
                     /gene="uxaC"
                     /locus_tag="SDA10_001882"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEG4240780.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            complement(3169..4248)
                     /gene="uxuA"
                     /locus_tag="SDA10_001883"
     CDS             complement(3169..4248)
                     /gene="uxuA"
                     /locus_tag="SDA10_001883"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG4240781.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(4251..5882)
                     /locus_tag="SDA10_001884"
     CDS             complement(4251..5882)
                     /locus_tag="SDA10_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEG4240782.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            6199..6870
                     /locus_tag="SDA10_001885"
     CDS             6199..6870
                     /locus_tag="SDA10_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4240783.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            complement(7706..8662)
                     /locus_tag="SDA10_001886"
     CDS             complement(7706..8662)
                     /locus_tag="SDA10_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240784.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(8771..9016)
                     /locus_tag="SDA10_001887"
                     /pseudo
     CDS             complement(8771..9016)
                     /locus_tag="SDA10_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9161..9583)
                     /locus_tag="SDA10_001888"
     CDS             complement(9161..9583)
                     /locus_tag="SDA10_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240785.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(9763..10134)
                     /locus_tag="SDA10_001889"
     CDS             complement(9763..10134)
                     /locus_tag="SDA10_001889"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG4240786.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
BASE COUNT         3193 a         2090 c         1485 g         3409 t
ORIGIN      
        1 tataataatc tggaaataca taattgttca tactcattgt taaaagctga gcaagcaaca
       61 aaaaaatagc tgcagctatt atagaaagca acgctctatt tttagctaat ccttttaaag
      121 atttcaacaa tgaaactttt aactgcgatt catcactttt aacccgttct acagttaatt
      181 tataacatat tacgtaacaa ataactgcac acactgaaaa tactcctgcc acaattgtaa
      241 aagttgatcc accacgtaca atttgagctc cagaactatc tcttgtataa ataaaaattg
      301 gtgttaacac gccaatgaac atacctgcta aggtagctcc tatactacga aatgttgata
      361 acgctgtcct ttcttctgca acagaagtga tagctgatgc cattgaaccg taaggaatat
      421 taatagatgt ataaaaaata cttccccata gtatatatgt tacatacata tatataactt
      481 taatactcat agaagctgat gccattgatg tttgatacat taaaaaactt gctaaagcga
      541 ctggaccact catccgtaaa atccacggtt taaatttgcc agttttagat ggttttgatc
      601 catccacaat acgccccatt gttatgtctg taactgcatc aataaatctt gctactaaaa
      661 ataatgttcc tacggtagct ccgcttatcc ccaatacttt tgtataaaaa atcattaaaa
      721 aagaactagc aaaaatgaaa gtgaaatcat tcccaaaatc tccaaacata tatcctattt
      781 tgtctcttag accaaatttt ctttccatat aatttgcgct ccttcctatt gttaagcgtt
      841 ttcattctgt tgaactgcca acttacgtac tatactctga agtataaaga gttagtattt
      901 attttttaca ttttatagta ttatttattt tttctcctaa taattcttta tctcataaaa
      961 cttatctata tatttactag ctattcgagt tacttcttca aaatcacctt tttcaacagg
     1021 agccaacaaa ttcccaccta caccaactgc aacaacgcct gcttcaaacc actcttctaa
     1081 gttatctaga cttactccgc cagtaggcat tatatttaca tatggtaatg gagcttgata
     1141 cgtttttaca acagatggac caaaagcaga acctggaaat agtttaatga tttctactcc
     1201 agcggtaagt gcctccttta tctcagtaat tgtcatacaa cctggtaaat aaggaatttg
     1261 atataaatta caaatctctg ctgtttgtcg atcaaaagat ggacttacga tatattctgc
     1321 tccagccata attgcaatac gagcagtaaa cgcatctaaa actgtaccgg ctccaattat
     1381 tacatctttt tgatcagcat attttttagc aatttctttg attatttctt cagcattcgg
     1441 aactgtaaaa gttagttcaa tccctttaat tccacccgca gtaattgcat ttattgcttt
     1501 aatggcctca tctttatttt ttccacgtac aaccgcaatg actccagccg cttctaactt
     1561 acttaaaatt tctacttttt tcatatctat ctccttcttt tatggataca tactctagct
     1621 agttgtacta acttacaata aataaattta agcttttatt ttttgtatta ttttctactt
     1681 tcgatttcaa aaaaattaaa ataattatac gcattacgat aacaaatatt ctccactatc
     1741 tgactaagat acatttcatc atctggaatt cgacctgctt taacccactc gcttagtaat
     1801 tgacataata ctcgacggaa atactcatgg cgaggatagc ttagaaaact tcgagaatca
     1861 gtcaacatac ctgtaaaatt cgacaacaaa ctttgttgcg ccatcgtagt caactgttga
     1921 cgcattcctt cagctgtatc attgaaccac caagcacatc ccaattggat tttttgaatc
     1981 ataccccctt ggaagctttg cattgctgtt gctagtggaa gccaatcggt tggatttaat
     2041 gaatataaaa ttgtttttgg taagcaccca ctcttttcag cagtgtcgaa caatgctaca
     2101 acattttgtg tcaaactagc ttgatccccc atcgaatcac caccgacatc tactccaatt
     2161 tgttccttta atatacttga attatttctt aaagcattta aatgcatttg catcgtccaa
     2221 ccatatcttg tatactcaga catcaaatgt aattgaatac ctgtaacaaa ttgtgcaatt
     2281 tctgataaat ggaaatccgt ttttcctgct aaagctttta ttaacagatt gtcgagtatt
     2341 ttttcatctg ctatttcata aaaataagta ttcaaaccgt gatctgctaa tcgacagcca
     2401 atttgatgaa aataatccac tcgttggctc aatgcctgct tcaacccatt aaagtttttg
     2461 attggtatac ccgctgcttg actaagtttt aaaatatagt tcgaaaatcc tgatgatttg
     2521 atactccaga ccttatctgg acggaaagta ggtaatacct tgaaagaatt atcctgttta
     2581 gaaagctcag tatgatatcg caagtcatct gtaggatcat ccgttgtaca aatggctttc
     2641 acatccatca tcttcagcaa ttcttttggg cgataggaat ccgtttggat tttttcgttg
     2701 gcacgtcgcc aaatttctgg tgcattcttc tcagaaattg tcaaatcaat atcaaagtaa
     2761 cgttgtaatt ccaaatgaga ccattcaaaa ataggattac ctggcgcctt ctccactgtt
     2821 tttacaaaag ctaaaaattt ttcataatca ttacctgcac cactgatcaa ctcttcttct
     2881 atcccattag ctcgcatcaa tcgccattta taatgatctc cagctccttt atcatttaac
     2941 catacctgag tcaaattctt gtattgctta ttttctagca aacatgctgg gtctaaatga
     3001 caatgataat cgatgattgg ttggtgttct gcaaatttat gaaaaagtct tttagctatt
     3061 tcattctcta ataaaaagtt cttatctaaa aacatattta agcactcctt tccttccctt
     3121 agtttttaga agagaaaatt tttcttctaa atagactcca ttatataatt atttactcgc
     3181 ccccttttca agcgcttccc aaataccatt taaataaact gcacctagag ctcggtcata
     3241 taacccataa ccaggtctac cggtttctcc ccaaatcatc cgaccatgat ctggacgaat
     3301 atagccatta aatccattat cgtacattgt ttttaatatc ttatacatat ctagagaacc
     3361 atattctgat ttatgcggcg cttcataaaa atcgagtcca ttatcactta aatatttgat
     3421 attacgaaca tgtgcaaaag gaatccgatc ccgttgacaa aattcagata gaatttcata
     3481 gacatcatta tctggtcgtt ctgcaatact tccagtacat aacgtaatac cattagaagg
     3541 agaatccact acattacaca accattctaa atcgtcacga tttttaacaa tacgaggtaa
     3601 tccgaaaata gaatatggtg gatcatctgg atgaactgcc attttaatcc caacttcttc
     3661 acatgtcggg ataattgctt tgagaaaata tgcaaagttc tctcgcaatt tcgcttcatc
     3721 aacttcttta taggcttcta ataattgctt gatttgtgat aaccgatgag gttcccaacc
     3781 cggcaattca aaattattag tagaattttc tacttttcta ataatttcct ctggatcagt
     3841 aggaatattt tttgaaatat aggctaacgt agaagaccca tcatctaaat gataatccaa
     3901 atctgatttt acccaatcaa agactggcat aaaattataa cagataacct caatcccaaa
     3961 ttctgccaaa ttgcgaatcg tctgtttata attctcgatg tattgatctc gtgttggcaa
     4021 accaattttg atatcatcat gaacattaac actctcaata actttcaagg taaggccatt
     4081 ggcttcgact tcatctttta aagctttaat tcgttctctc ggccagacct ctccggctgg
     4141 tatatccatt aatgttccaa caattccttt cattgctggg atctgacgaa tttcttgtag
     4201 tgagattttt tcttcatttg ccccatacca tctaaaagtc atttccatag ttactttcct
     4261 ccaacttctg ttatatattt attgattgtt ttacgaactg cttgaggacc tactagcatt
     4321 tcgcaaaaga ttttttcaat tttctttcct atctcaactt gatataaatc taaaccaaat
     4381 atttccttat tttctaagat ttcctgtacc gcacaatgaa tctttgcagg atcttgttca
     4441 cctaatttca atactgagaa tttttcctgc aactctacca ataaaggatc aggacttggc
     4501 acaaaatttt ttccttgatc atcgactgcc aataaataac gtaaccaagc agcaatagcg
     4561 ataggaataa atgttagatt ttctgcattt ccgtcttttt cgacatactt tttaattgtt
     4621 tcaccatagc gaatcgctac tttttgagaa gtatctgttg caattcgttg cggtgtatct
     4681 ggaatatagg gattaggcaa acgtttattg attacttcat caataaattg ttttggatca
     4741 ataacgcctg gactttcaac tactggtaag ccttctacat aacctatccc tttaattaag
     4801 ccaaccaaat catcatcttg catttctgcc tgaattgatt tataccccaa caaacaacct
     4861 gttattgcta atgccgtatg gagcggattt aaacatgtcg taaccttcat gatatctgct
     4921 ttatcaactg tttctctatc tgtcataatc acaccagccg cttccagttt aggtcgacca
     4981 tttggaaaac tatcttcaat gactagataa tggacttttt cagtattggt aaaagcagcg
     5041 atattggtat ttttcgcagt atgaattata tctaagtctt cgaaacccgc cttcaataaa
     5101 tccattttga cactttccga aggatttggc gtaattctat caatcatact ccaaggaaaa
     5161 gtcacttttt ctttatcctt taaataagca ataaattttt cagaaacgta tccttttttt
     5221 tgccaagctt ctgcaatcat aaaaatgcta tcttgaaaac gttgaccatt ctgtgagaaa
     5281 ttatcagtag aaaccatcgc aatcggtaac tctccagcat taaacctgct atacaacaag
     5341 gatgtcacaa tactcatcgt atgttgagga ttggtaggtc cagattttat atctgcttcc
     5401 actaccgaaa aaaattgtcc tttagaatct tttaaactgt atcctttttc agtaatagta
     5461 aacgttgtaa actgtaatgt aggatcttca aaatatttaa ccactctttg atagtccgtt
     5521 atattctttg gattgcaata taaactatca gctgttactg ctaaaagtct ttttttgagt
     5581 tcaccatctt cacccataac aatctccaaa atatcattgt tgtagtcatg ataagctttt
     5641 tctactactt cttcatcaaa tgtctcacac acaactactc cggattctaa ttcattttga
     5701 tctgctaagc attgagcaat ttctgcatga aatcctcgat aaagatttcc tccaccaaaa
     5761 tgaagccaca ctggatgttt ctgaccttct tgtttgaccg cttcgacatt aaaatacggt
     5821 agttgaactt gcattttgtt caatccttcg atagaatctt gccagtcttt aatcaaaacc
     5881 atatttaccc ctccatactg atatgttagt ttccgttaca attgtagtat aaaatcgaat
     5941 gtaatcgctg tcaataagaa aaaactaaaa atattctttt ttctaccatg tcagaattat
     6001 tgtgtctttt tttagtaaaa gcacaccaat atatattttt tagaactaac ttaaattaaa
     6061 gcttaatttc tctttgatct tatttgagta ttttttgttt ttttaatagc tagtatgtta
     6121 ctttgaaatt tgatattata taacgagcat agaactaaaa tatcaaattc aaaaatgaaa
     6181 ttttgagagg gattaaatat gacaattaaa actagtctcc aaaatcaagc ttatcaatct
     6241 attcgtcagc aaattatcta cgctgactta gaacctggaa aaaaagtatc agaaaaaaaa
     6301 ttggaagaaa cgctttctat cggtcgtact ccaatacgtg aagcattgat tcaactccgt
     6361 catcaaggac ttgttgatac aattcctcaa agtggaaatt atatctcatt aatcgactta
     6421 gatttagcaa aaaatgctcg ttttgtcaga gaacatttag aacgacaaat tatgctagag
     6481 tgttgtgcca agatgaataa tcaaaagaaa caagttttag aaactataat tgcagaacaa
     6541 gaaaaagctg ttagtcaaaa gaatgctcgt agtttttttc ataatgataa tttatttcat
     6601 aaagcttgtt ttgaaattgc agaacgggaa gaagtatggc aatggttgga agataataat
     6661 acgcacttgg aacgatttcg ttggttacga gtaaaaacgg aaggattaaa atgggaaaaa
     6721 attatggaac aacatcatca actatttaaa gccttgacca tgcaagaccc tgaagaagct
     6781 aacttcttaa catccttgca tcttcattta atgcttgatg aacaaaatgt tgtattgact
     6841 actcacccaa aatattttaa aaatatataa ctgctaaatt tctttttttt aatttattta
     6901 catttctgat tactacagaa aactaactac tttttttatg taaagttctg tcaaatatag
     6961 tcactttata ttactttctc ttgagtaaaa aaacatgtga ggtcgtaaac aaagattgaa
     7021 ctatctacat tcatactcta taatctgttt atttgcagac tgataatagc aaaagaaaac
     7081 ttataatcaa ataatatcaa catttgatca cataatttga gattgaatgc tgatttacct
     7141 atgttttacg taatggtctt tagttatttt tgctcataaa atgcataaaa cacgcgtttt
     7201 tatgtgtgct tgaaatttct tggctctata atttctaaga ctgataaaaa tattctatcg
     7261 gttctttttg caaataaaaa aacatataga agctccatta gattgaatca ccacaacaca
     7321 atcaaaggag cattccgtat gcttgatttt cattctataa aactactttt aggaatcaaa
     7381 gaaaatcaca acggttcaaa ctaaaagcaa gaatatccga agtaccattg tttcatcgat
     7441 catgagaaaa gcattcacct ctgtctgcag atgttcttat atcgctctgt cagtcctatt
     7501 aataaagtac tgttttgttt tcaaaaaaac tattacgagt actttttaag agaatgcctt
     7561 gcaaatattc atcacacacc gttatataag tcaaatagtt tctttcagtc gtcaagaaaa
     7621 gattctcctg tccttggcat tatcagttaa gtagcaactt aattctcgga cttgttctac
     7681 tctagcaaaa tttattttca tcaacttacg tccaaatgtt tttttgaata attacttcat
     7741 cgcgggtctg gaagacttgc ctcattcgat aagtcactag cttttggcgt ccttcaggat
     7801 ttctagtata caccaactga catttgggca gcgacgattg atttaattcg tttagaaaaa
     7861 ggttagcgat ttgcttattt cggccaaagc gttctggtac cgtcagaact atctttttcc
     7921 cttcgattaa ataccgcgga ttctccagcg gtgctaaaag ctccctcata gcttcagtaa
     7981 aaagctgtcc ttctcgatag ccagcattct caagatagca tctaaattcc tgctgatcag
     8041 ccgctacgat tacttttcta tcactaactt caccaaggcg ctgcaacgtc gtttgaacgc
     8101 atatggccaa gctttttttg cgggcacatt ccttttccca tagcttttgt cgcctacgaa
     8161 tttggaaata ttccagcaag ttacctttaa atatagctga ccagaagctt agaggctcta
     8221 tattttttac tactatcggc aatgaagttt cttgtttttg ctttacctga tcgactttcc
     8281 taacgacgat tggcatagat aggtgccagc catcagctaa agcagtactc cattgttctt
     8341 tcaaccgact tcgttcacaa gaaaaataga acatcgactg agtataagtt tccatcgtgg
     8401 tggtcgtata ttccagtggt aaagcaaagc gatcgccacc actctcaatt tttggtgaag
     8461 attcaaaggt cagacctgaa atattgccca aacgccgtag cattcttttc aaatcaggct
     8521 gttcagacag aggaactccc ggtaatccaa tcgctaaatg ccaaatgttc gttactttat
     8581 tttgtgcacc taggcgtaag ccccgccctc gtagttgttg tgtttgcaca aaagtggaag
     8641 cttgattagc caagatcaac agattaattg caggacaatc ccagccttct cctagcaaac
     8701 taacggttcc taccaaaata ttcaacttct tttcggttaa taggcgagta actgcttgca
     8761 tcaatgcttg gcggtttttg tcatccaccg tcaaataaaa ataacccgac atataaggat
     8821 gttcattcat acccgtcgct agttctgcca gctcaacagt tactagcatt ttcggtaaca
     8881 gtaagaactc tccacagatg gcaccgattt gctgagtgtt acctaataga cgaacaactt
     8941 catcaaacaa aggaaccacg ccataagcta aggtaccatc actgcctgct aatacctcct
     9001 tgcaaatacg atccatcaaa atgaccgctg tcagttcttg cttcagctga acttcttctt
     9061 ttaacaaaat ttgacaaact gcttgctttt tctcaggaat cgcctgacta ccaacagtgt
     9121 ctggatactc tggaaacaaa tgcagcgact catcaaacaa tcatccagct tttttcaatt
     9181 gataaaaaat aggcaattcc ggttgatctt caaacatctg ctgataaata gtgatcagct
     9241 cttttttacc taaagatgga actgaagctt ttttaccttg ctgcttagaa ctgcctaata
     9301 gctgccaatg ttcgggtatc aattcatagt tatgatgatt caagtaaatt aatgcactag
     9361 tgaacagctc aaaattttca taaatttcct tcgaatgctc aagcggatgc tgaacaaatt
     9421 ctaaagaaag caaataatcc accgtctctt ggctttccat taacgcttcc accagctcat
     9481 tttgtttctc taaaaaggta aaataatgct gagtagtttc ctgattagcc ggaatcaaat
     9541 atagaaaatc ctgatgagga catagaacct cctcctttac caactcggga atgctaattt
     9601 cttcgtcaat caggccattt taactcaata tattgcttcc actccgtctg agagctatca
     9661 atcggaggcg tggctgtcaa ggaaatcgtc aacagattcg gttgtttttt cttcaagtca
     9721 atcaacaatt caccccagct tttttttaaa tgatgagctt catcaaaaaa ctaacagctc
     9781 cagctgatca ccataagttg gctcctcctt cagtcggttg taaatggtct gatacgtttc
     9841 cactgtaata tgactaggat gttcaatcgc cccagaaaga tagcaactat ctgctggatt
     9901 tactaagaaa tattcctcga ttgttgttaa ccactgtttc ttcaatatta gggaaggaac
     9961 caaaattaag gttttacaat ccaaacgtcg gatcatctca attcctaaaa aggtcttgcc
    10021 agcccctggt ggagccacta gatggactga attgttattt aaataagtag caaagttagt
    10081 caatatacgc ttctgatagc tacgccactg aattttttca tcagatagcc acatcccagg
    10141 tccccctctc acgtattttc tctatcataa tctaaat
//
LOCUS       DARDHA010000094        10114 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000094 DARDHA010000000
VERSION     DARDHA010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.095"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            240..1580
                     /locus_tag="SDA10_001890"
     CDS             240..1580
                     /locus_tag="SDA10_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG4240787.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(1650..4031)
                     /locus_tag="SDA10_001891"
     CDS             complement(1650..4031)
                     /locus_tag="SDA10_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEG4240788.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(4021..4668)
                     /gene="recU"
                     /locus_tag="SDA10_001892"
     CDS             complement(4021..4668)
                     /gene="recU"
                     /locus_tag="SDA10_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEG4240789.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            4732..5271
                     /locus_tag="SDA10_001893"
     CDS             4732..5271
                     /locus_tag="SDA10_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEG4240790.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            5361..5780
                     /gene="gpsB"
                     /locus_tag="SDA10_001894"
     CDS             5361..5780
                     /gene="gpsB"
                     /locus_tag="SDA10_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEG4240791.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            5802..6169
                     /gene="rnpB"
                     /locus_tag="SDA10_001895"
     ncRNA           5802..6169
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="SDA10_001895"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            6378..7544
                     /locus_tag="SDA10_001896"
     CDS             6378..7544
                     /locus_tag="SDA10_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEG4240792.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            7590..9086
                     /locus_tag="SDA10_001897"
     CDS             7590..9086
                     /locus_tag="SDA10_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEG4240793.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            9167..9850
                     /locus_tag="SDA10_001898"
     CDS             9167..9850
                     /locus_tag="SDA10_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG4240794.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         3162 a         1908 c         2124 g         2920 t
ORIGIN      
        1 cttataaaaa atcatgtaat acagcttggt acatgatttt tatttgtagc tgttttttac
       61 atagacacaa aaaagaaacc acgcttgtag acgtggtttc ttttttagga tctataatat
      121 ctagtgttgg tgacgccttc tgttgccaat actttttctt ttttctaata aacgcaaaca
      181 gacaccttga aatcctttag aataaagtcg acgaaaacca ttcaaaggag tgtttcaaga
      241 tgtcctattc agagtttacc aaagagttac tagatattct agacctaaat cttacctttc
      301 atgaagatgc ctttcgcaaa gagcgcatta atgacgaaac atgttttgtt tttgatggca
      361 cattgaccta tcaaccagag gaatgcttcc attgccacta tcaaaataag caaacgatca
      421 ttaaatgggg ctggaaaaaa gtatccatct tattgaatga tgtcagtaac tacaagacca
      481 ttgtgcggat taacaaacag cgcttcaaat gtaaacactg tggaaagact tttttagcag
      541 aagattcagt tagcgatcgt cgctgctcca ttgcccgcag agtcaaacaa gccattcttg
      601 agctattaag tgagcctatt tctatgtctt tgattgcacg tatgaagcac atttctccga
      661 ccactgtcat tcgaatactt cgcagccttc ggcctaaaac cgtttctttg aaccagccgt
      721 taccagaagt ggtgtgtttc gatgaattca aatcggtgaa aaacgtctcg ggagccatga
      781 gctttgtcat gatggacggc aagacccatc aactgattga tattgtggaa aatcggcaac
      841 tcaatccttt acgcgactat ttcttgcgct accctagaaa agtccgtgaa caagtacgtc
      901 tagtggtttc agatttctat acaccctatc gcacattggt gaaagagtgt tttccgaatg
      961 cccgaatcgt cgccgatcgc tttcatatta gccaacacat cggtcgggcc tttaccaacc
     1021 accgaattca agtaatgaag acctttaaaa aaggggatcg tcggtcgaag cacttaaaga
     1081 aatactggag actacttcaa aagaatgcgt gggaattgaa tggtcagcat cgctactggc
     1141 gtccgtcatt tcgagatcat ctgacagaag cggagatagt cgatcggcta ttatattatg
     1201 atgactcctt aaagcgaggc tacgaagtct accaggtttt tctttccgct atcagaagac
     1261 aagatgttcc tgaattcgtc gcgctcctaa aagaagacta caaggagttg ccagagcact
     1321 atcaaccagt gttcacgacc ttcaagaaat atcgaacaga aatcaaacga gcgttgcgag
     1381 ttccctattc caacggccct atagaatgtt tgaacaacca tatcaaagta ctaaaacgaa
     1441 ttgcctatgg ctttagaaac tttcaaaact accgagaacg aatcttttta tatcgaggaa
     1501 aatatttcaa gaaaactaag aacactaccc aactgactaa agccagaaca acaacacggc
     1561 tagacaagat agctgtctag ccgtgtgagt atctcgatta ttctattgaa tttcttcacc
     1621 aacactcatt gacgaagagc ccttttttac tatctgtttc cagaagctga ggtgctgctg
     1681 gatggtgtat ggtcatttgc agcatccgct ccgctagttg ctggctcact ggaagaagca
     1741 ggaggagcag atgaagacgg tgtcgagctt tcttcggatg aagaagaagg aaccgttgtt
     1801 gaagacgctg gctcgcttgt ttctgctgtc gtgctttctt ttgaggactc agcagttgat
     1861 tcgctttgac tagtagagct agagctttga gtcgttgtct ctgttgttga gcttccagga
     1921 gtctggacat agctggaaga tggaatagtt gttgatggtg taattgcatt cgatctagct
     1981 gtatattgat ctttgtagta cagttcaccg cccacacgaa ttaatccgct tggcatctcc
     2041 cagtctgtat ttgtcacgtt agctgaaaca tactgcataa gctcgcgata aacatcagag
     2101 gctacgtgtg acgattctga agtgacaggt gtcatttttt tattgtaacc tgtccacacg
     2161 gaaatcgaat aatttggcgt atagcccgca aataggatat ctggatacac cccagaagaa
     2221 ataccaagtt ttgcatactc gtcatctgtg taattggatg ttccggtctt accggcttga
     2281 tataacccag cgatttgcgc attcgtacca gttccttcag tgatcgtatc ttttaaaatg
     2341 tctgtgatca tataagctgt ttctggagac atggctgttt ttccatctgg ctcataagtt
     2401 tcttctgttc cgtcttggaa gacgatttta ttaacatatt gaggtttgta ataagtccct
     2461 ccattggcga aagccgcata ggctgctgcc atttttaagg aagaagctcc gtattttgtc
     2521 ccatcttgtt cttccgtatt actagagatc gcattcgatt gatggatcgt actgtattcg
     2581 atcccaagat tttttaagaa agaagccact ttatctgaac cgacttcatt gaataatttg
     2641 actgctggaa cattacggga taagtaaagt gcttggcgta aagtaatggt gcccatatat
     2701 tgattatccc agtttcctac aggagtcgaa gtcccttcat agttatacgg tgcgtcagta
     2761 atagtttttc cagtagaata tttcaaatat tcaaaagctg gtccgtaatc tgtgacaggt
     2821 ttcatcgtag aaccaaaatc acgggaagta ttcactgcta agttgttgcc taaagttacg
     2881 tcttctgcga tgtgacgtcc gccaatctgg gcttttactt tcccggtatt ggtgtcgata
     2941 agggtggagg caacttgcat ctcgtcgtct gggtaagaga catactgatc cgtgttgacg
     3001 atatcatata gtttcttttg ggcgtccaga tccaaattcg tataaatctc taaaccgtct
     3061 gtgtagacat tcttatctgt tttttcttgt acttcattga tgacttcttt gacataatta
     3121 tcgacgatct tagtattgtc atcggattga gttagttctt gcagaccatc cgttaccggt
     3181 acattaacag cttgatcata ttctgtttga gagatttttt cgttttgaag catcgtatac
     3241 aaaacagtat ctcgtctttt cttcgcctga tctggataaa cataaggatc ataggcggaa
     3301 ggggcttgag gcatcccagc aagcaatgct gtttgcggta aagaaagttc tgataatttc
     3361 ttaccaaagt acatctcaga agctgtctcc atcccgtaca agccgtttga catatacact
     3421 ttatttacgt agtaggtcaa gatctcttgt ttcgattttt tttgttccaa tcttacagcc
     3481 atccacgctt cttgggcctt gcgtttcaga gtctgatcct cggcactggt ggagaagaag
     3541 gaaagtttga tcaattgctg tgtcaaagta cttccgcctt gcagtccgcc agaggtaaag
     3601 ttagagaggg cagaaccgat gatccgaata gggtcaaccc cgatgtgttt ataaaaacgg
     3661 cgatcttcta ccgacacgat cgcatcttct aatgttttcg gtaattcatt cgcagaaatt
     3721 ttttcacgct tttctgcgcc taaatcttcg aatagttcgc catcttgtgt atatagtttg
     3781 gaagatacgg tagcgtctaa ttttttatcc gtcaattcgg gcgcatcttt tgcgtaatac
     3841 cagaaaagtc ctacacctgc taaaaagagg atacagaaaa atgagagtat tcctaaaagg
     3901 atcttgataa ataatccttt tttatgtgtt ccagaggact ttccagaacc tttgccagaa
     3961 ttcttcttca cactcttttt aggagtaggc tgtttctgtc gtctagagct tctagtttgt
     4021 tcatttgcca ttcttgcttt ctccttcaat agataatgtt gataaataga agtcaaccgc
     4081 gtccaaataa ggaatccgcg gagaaatacc agttttgatc tctattccta gttcttcgat
     4141 caaagataaa ggtagtgatt tgcgtccttt ttccttttgg gcgtcccagt aagcaatcaa
     4201 atgaacacta ttcaaataaa aacagcgctt caaagacgaa aaccataaca agacaaagca
     4261 gaccccattt tgttcgaggc atgctttcat atgttcgatt tgatgggcat ggaagttttt
     4321 aaagggaaaa gatgttttgt tcttggtttc ttttgcttca aagtccaagt aaaagcctcg
     4381 atagacgcca ttatagtctg tagttgaggc ttgggtaaag taggcttcct tgatcacagc
     4441 agcacttctc ttaggatagt ctactttcac gatttggata ggggtaggct ttttatggat
     4501 aaccgccaac ccgcgggaaa gataatattt attactttca ttgattgctt cttcaaagcg
     4561 catcccccga ttgccgaatt cagtgatctg atttcttttt tcttgaattt tttcgacggg
     4621 agaggcattc ttataataag gttggccatt tggatagcgc aataccatct ttaatcacac
     4681 tcctaggcca cattatagca aaaaagcgga tagaaagaaa aggaatctga gatggataag
     4741 tcaaaagtaa tctatgtgtc tgggtatcga agtttcgaac tcggtgtttt taaagaaaat
     4801 gatcctaaaa tccaagttgt taaaaatgtt ttaaaaaaag aactccatca gttagcagaa
     4861 gaagggctgg aatgggtact tgtcagtggg aatttgggcg tcgaactatg ggctgtgcaa
     4921 atagtagcgg aattgaaaga agaatatccg gaactatctc ttggtatttt gtatcctttt
     4981 tctgattttg gtaataactg gaatgaaggg aaccaagaaa aaaaacaatt agcagaacaa
     5041 ttagcagatt atgtcgaagc tgttagccat caaccctacc aatcacctag tcagctaaag
     5101 aatcacactc gttttatact tgagcataca gtgggctgtc tgctgatcta tgatgaagaa
     5161 ttccctggaa aaacgaagta tttttttgaa gatgcacaga attaccaagc agatcatccc
     5221 tacgaaattc ggctgatcag tatggacgat cttcaaaatt ttagtgaata atcgatattg
     5281 tgtttgattt atcaactttt ttcctataca atgaaaaagg tagaaatgtt attacgtaaa
     5341 tgaaaactag aggtgtaaat atggcgaatt tggtttatag tcctaaagac attttacaac
     5401 aagaattcaa aacaaaaatg cgcggctatg atcccgttga agttgatgaa ttcttagaca
     5461 atatcatcaa agattatgaa gcttacagca aagaactcct tgctttacaa gaagaaaacg
     5521 atcgattgag tgcaaaagta gctcaattat ctaaaactca aggagcagct caaacacgcg
     5581 tgcaacaaac agaggcacca aagagcgcag cagtgaccaa ttttgatatc ttaaaacgct
     5641 tgtcaaatct tgaacgtgaa gtatttggta aaaagcttga tcaacaagca agtgcagtca
     5701 aaccagcaca acctaatcca aataactaca caaatgcaga cacaagctta gatgataatg
     5761 aaaaaacacg tcaattttaa tttttgatta atgaatttgc ggttttcggg taatcgcggt
     5821 ctgacttgat caggctgagg aaagtccatg ctcgcacagg ctgtgatgcc tgtagtgttc
     5881 gtgcttagcg aaaccataag ctaaggtact ctttggagta acggcaggaa aacaagctaa
     5941 ggcttcggct atgcttaagt atccttgaaa gtgccacagt gacgaagcgg ctggggaaac
     6001 ccatccggtg gaacgcggta aacccctcga gcgagcaacc caaacaatgg taggggcgct
     6061 ttttgtctgg aagaagaaca ggacaaaaag gcagtataac tgcagataga tggttaccgc
     6121 tttgtttttt ctccctggaa aagcaaagta cagaacatgg cttatagaaa accgcaaaca
     6181 tcagggaatt aggtcgtgac aaaagtaatg cgttataaga aacaagaaaa acatttgaaa
     6241 gctatttttc aatacttcag atgaatcgtt cttattacaa aacaaacttt tgtatcaccg
     6301 gttattcgga atttgagtaa aagtgtgggg taggactgat gtcccacctc ctttttttat
     6361 aaataaggag attgtgtatg gaaaaaaaca ggaagttcaa cctcgtcgca actgcggcaa
     6421 gcgggctaga agcactggtt ggtaaagaat taagagatct tggaatcgaa tgccaagttg
     6481 agaacggacg tgcacgtttt gaaggaacaa tggaaacgat cgctacagct aatttatggt
     6541 tacgtacagc agatcgagta aaaatcgtgg ttggggaatt cgatgcgtat acgtttgatg
     6601 aattgtttga aaacgtaaaa gcattgcctt gggaagattt tcttccacta gatgcagctt
     6661 ttcctgtagc tggaaaatcg atcaagtcga aactatatag cacacctgat tgccaagcga
     6721 tcacaaaaaa agcaatcgtt gaacgtctcc gtacctatta tcaccgtcca gcttctgtac
     6781 cactgacgga aactggcgca cattttcagt tggaagtagc tttgctgaaa gaccatgtga
     6841 tggtaactct ggatacgaca ggaccaagtt tattcaaacg tggttatcgc ttagaaaaag
     6901 gtggagcacc attgaaagaa aacatggcag cagcacttat tatgttgaca aattggcgaa
     6961 aagaccgtcc attttatgat cctgtatgtg gttcaggtac gctttgtatt gaagcagcct
     7021 taataggcca taatatcgca ccaggattca atcgtgagtt tgcttgcgaa tcttgggatt
     7081 ggttcagtca agaaatcatg gaaaatgttc gatcagcagc cgaagaaaaa gcagattatg
     7141 atattgaatt agatattact ggttcagata tcaatggacg gatgattgaa attgccaaag
     7201 ccaatgcaga agaaatcgga ttaggctctt cgattacctt taagcaacaa gcagtcaaag
     7261 acttcaaaac ggataaagaa tacggggtca tcgtggctaa tccgccttac ggagaacgtc
     7321 taggagaaga agaaactgtt cggaaattat ataaagaaat gggcgatgta ttccgtccat
     7381 taaaaacatg gagtaaatat atcttgacta gcgacttagc gtttgaggaa ttttacggac
     7441 aaaaagcaac caaaaaaaga aaattgtaca atggtgcatt gcgtacggat ctattccagt
     7501 attggggaac tcgtccgcct agaaaaaaag agaaagacga ataatcttac taattgattc
     7561 attcaattgg atgtttggag gaatagggaa tgaaagaaaa agagttttta caagaagtaa
     7621 aagaaatcaa tcttctacaa caagctttag gaatcctcga ttgggataca caaacaggta
     7681 tgcctgaaaa agctagtgca taccgaagtg aagtagacag ctatctttac agcatttatt
     7741 tttcaaaaaa aatcggtcca aaaatcaaag aagccattcg ctatttttct gaacatccgg
     7801 aagaattgtc ggaagttgga actgccgttt ttgccaaagt aaaagaagag tatgatttgg
     7861 aacatcgcgt accggaagat cttatgagaa aatatacagc tgccgtttct tcagcacacg
     7921 gacaatggca aaaagctaga gaaacacaac aattttctga ttttcaacac gcattgaatc
     7981 aaaatattga attaacaaaa cagttgatac cttactggag aaaagatgaa aagacgaatt
     8041 atgatgtttt gctgaatcaa tacgaacccg ggatgactgt cgaaatcctt gataaggtat
     8101 ttgatcagct taaagaagga attctttcga tccgccgtac gctccaagaa aaagggactg
     8161 aacctgaaac agacttttta aatcggagaa tgacgaaaaa acaacagaag agatttgtag
     8221 tgaaagtaat cgaacaactt ggttatgatt ttagtcgtgg tcgtttagat gacactgtcc
     8281 acccatttat gactggaatt aatccgaatg atgcacgaat cacgacacga tggaacgaaa
     8341 cagatttcaa aatggctgtt tttggagtga tccacgaggc tggtcatggt atgtatgaac
     8401 aagacatcga tgataaatat gcgtatacac cggtttatga aggtacatca atgggtatcc
     8461 atgaatcaca atcgttgttt aacgagatca ttattggaag tagtcgcagc ttctggaaaa
     8521 agcaatatcc tttctttcaa gaatgtgcag aaggaacatt cgatgatatt ccttttgaca
     8581 aattttatcg ttcattgaac catacaaaat caagtttggt tcgtatcgag gcagacagtt
     8641 tgacttaccc tctccatatc atcattcgtt atgaaatcga aaaaatgatt ttcaatgaag
     8701 gggtagatgt tgctgaacta cctgaaatat ggaatcaaaa atatgaagag tatctgggaa
     8761 tacgaccaac aaatgatgtg gaaggcatcc tacaagacgt tcattggtca ggtgcaagct
     8821 ttggttattt tccctcttat gcattagggt acatgtatgc ggcacaatta cttcattctt
     8881 tgcagcaaga aatagatgtg gaaaatgttc tagcgtcaga cgattattct ccaatcaaaa
     8941 actggatgtc agagcatatc catcaatatg gtgcatctag aaaaccaaat caattaatca
     9001 tggatgcaac acatgaaccg cttaatccgc aatacctgat cgactatatg aaagacctgt
     9061 attttaatgt atatcaggta aaataaaata caatttacaa agatagctga aattcatcta
     9121 atctatagat gttttcagct atttacttaa gaaagaagtg gaacgaatgg ataaattaca
     9181 agtgatcgct gattatagta tggaccaatt agcgcatgat caaacaggac atgggagcga
     9241 tcataccaaa agagttgtca agttagctga aaaaattttg gagacggaac cgcaggcaga
     9301 ccgctttgtg acgcttgcag cagcttacct acatgatacg attgatgata aagtcgttaa
     9361 aaatgagaac gaggcaaagc aacagcttcg tgtattcctt cgctctttgc caatagtaga
     9421 ggaacagatc tcgatgattt tttccattat cgaaaatatg tccttttcta aaaatctctc
     9481 tgaagcggcc gaactatctc tggaaggtaa gatcgttcaa gacgctgatc gtatcgaagc
     9541 attaggagca atcggcattt tgcgtactgc ttatttcggc ggtggacatg ggcacccgat
     9601 ttttgatcct gaactgtatc cgcaaacatt caaggacaaa gaaaattacc gaaaaggcac
     9661 gacagtgatc aatcattttt atgaaaaact gttcttgctg tctgataaaa tgaatactga
     9721 ttacggatat gaagaagcca aacgaagaga aaccttcatg cgtgaattct tagaagaatt
     9781 ttttctggaa tggtctattg acgatgaaaa ttttactcca gacaattgga tcaaaaataa
     9841 caaaaaatga tggaggttgt gacagaagtg gtcagctcca agatatttcg gatcattttc
     9901 cgtaaaagtt acttctggaa cactgtttac caagaggtcg tgagcctgcc gttcagctcc
     9961 gagtcacaag gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat tgtaacttgt
    10021 gattcggaat tggcaggaga acaccgttta ttcggaagaa taggactgtg acaagacttt
    10081 tgtcacagtc cttttttcga attcaacaag taaa
//
LOCUS       DARDHA010000095        10086 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000095 DARDHA010000000
VERSION     DARDHA010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.096"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(247..759)
                     /locus_tag="SDA10_001899"
     CDS             complement(247..759)
                     /locus_tag="SDA10_001899"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEG4240795.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="SDA10_001900"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="SDA10_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEG4240796.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="SDA10_001901"
     CDS             complement(3212..3655)
                     /locus_tag="SDA10_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEG4240797.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="SDA10_001902"
     CDS             complement(3656..4444)
                     /locus_tag="SDA10_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG4240798.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="SDA10_001903"
     CDS             complement(4441..5382)
                     /locus_tag="SDA10_001903"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEG4240799.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="SDA10_001904"
     CDS             complement(5559..6146)
                     /locus_tag="SDA10_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEG4240800.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="SDA10_001905"
     CDS             complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="SDA10_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEG4240801.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="SDA10_001906"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="SDA10_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEG4240802.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="SDA10_001907"
     CDS             complement(9063..9998)
                     /locus_tag="SDA10_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG4240803.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DARDHA010000096        10084 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000096 DARDHA010000000
VERSION     DARDHA010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.097"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            162..392
                     /locus_tag="SDA10_001908"
     CDS             162..392
                     /locus_tag="SDA10_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEG4240804.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="SDA10_001909"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="SDA10_001909"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEG4240805.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="SDA10_001910"
     CDS             1803..1979
                     /locus_tag="SDA10_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEG4240806.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="SDA10_001911"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="SDA10_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEG4240807.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="SDA10_001912"
     CDS             2474..2941
                     /locus_tag="SDA10_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4240808.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="SDA10_001913"
     CDS             3026..3910
                     /locus_tag="SDA10_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG4240809.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4024..4260
                     /locus_tag="SDA10_001914"
     CDS             4024..4260
                     /locus_tag="SDA10_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEG4240810.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="SDA10_001915"
     tRNA            4348..4428
                     /locus_tag="SDA10_001915"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="SDA10_001916"
     tRNA            4435..4506
                     /locus_tag="SDA10_001916"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            4631..4801
                     /locus_tag="SDA10_001917"
     CDS             4631..4801
                     /locus_tag="SDA10_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240811.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5021..5728
                     /locus_tag="SDA10_001918"
     CDS             5021..5728
                     /locus_tag="SDA10_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4240812.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="SDA10_001919"
     CDS             complement(5788..6432)
                     /locus_tag="SDA10_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEG4240813.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="SDA10_001920"
     CDS             6559..6846
                     /locus_tag="SDA10_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240814.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="SDA10_001921"
     CDS             6954..7274
                     /locus_tag="SDA10_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4240815.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="SDA10_001922"
     CDS             7355..8668
                     /locus_tag="SDA10_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG4240816.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="SDA10_001923"
     CDS             8665..9384
                     /locus_tag="SDA10_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEG4240817.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="SDA10_001924"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="SDA10_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEG4240818.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         3359 a         1668 c         2151 g         2906 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaa
//
LOCUS       DARDHA010000097        10037 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000097 DARDHA010000000
VERSION     DARDHA010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.098"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            54..560
                     /locus_tag="SDA10_001925"
     CDS             54..560
                     /locus_tag="SDA10_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEG4240819.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            639..1361
                     /locus_tag="SDA10_001926"
     CDS             639..1361
                     /locus_tag="SDA10_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4240820.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(1439..2839)
                     /locus_tag="SDA10_001927"
     CDS             complement(1439..2839)
                     /locus_tag="SDA10_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEG4240821.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(3009..3983)
                     /locus_tag="SDA10_001928"
     CDS             complement(3009..3983)
                     /locus_tag="SDA10_001928"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEG4240822.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            4336..4896
                     /locus_tag="SDA10_001929"
     CDS             4336..4896
                     /locus_tag="SDA10_001929"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEG4240823.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            4913..8434
                     /gene="mfd"
                     /locus_tag="SDA10_001930"
     CDS             4913..8434
                     /gene="mfd"
                     /locus_tag="SDA10_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEG4240824.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            8449..>10037
                     /locus_tag="SDA10_001931"
     CDS             8449..>10037
                     /locus_tag="SDA10_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG4240825.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKI"
BASE COUNT         3036 a         1849 c         2222 g         2930 t
ORIGIN      
        1 aaatgattgt tgtttcttca aaaaaagtct aagataaaat acagaggtga cttatgaatt
       61 cttcaaaaaa acaaatgaaa aacggaaata tttatttagt aatcgcactg atagtcgtta
      121 taatcttatt ttacagttct tctcaaactt acggacagca atcacaagtt tcgcgattag
      181 aaagctggct gcctaatcag ccatttaaag acacattagc gggtatacgt tttaattatg
      241 gtggaagtat ggtgagtatc aaccatctgg gctacgccaa atttttagag ttctttttaa
      301 ggaagggcgc acatttcggt acctatttta ttttgggagg cagcttgttt ttaggtgttt
      361 tccccaagct aaaaatttgg tggctgacag cgatccttgc ttggctttca gcaacagggt
      421 acgcaggttt ggacgaattc catcagatga tgaccggtgg acgaacacct atgttccaag
      481 atgtcatgtt ggattcgatc ggtgctctga ctgctgtgat attatgtgtg ctttttgcgg
      541 cattcaagaa gcgtttatag attggctgtg gctttgtttt tttgtgaaat agtactagaa
      601 tagaagtgta ttctcaaaag aacaagggag gaaaaatcat gtcaggacat agtaaatggc
      661 ataatatcca aggaagaaaa aatgcccaag atgcaaaacg agggaaagtc tttcagaaac
      721 tttctcgtga aatttatatg gcagctaaag caggcggtcc tgatccatct atgaatccag
      781 ctttgcggct tgttgtggac aaagcaaaag ctgcaaatat gcctaacgac aatgtggaac
      841 gggcgattaa aaaggctacg agtgccgtag atgaaacgaa ttacgatgaa atcacgtatg
      901 aaggatatgg tccgggtgga gtaggaatct tagtccatgc attgacagat aaccgtaatc
      961 ggacagcgac aaatgtccga gtagctttta ctcgaaacgg tggatcactg ggagaaacgg
     1021 gttcagtcag ttacttgttt gaccgcaaag gatatatcgc tattgaacgc gatgggttga
     1081 ctgttgatga agagacgatg tttgaggatg tattagaggc gggggcagaa gatctgcaaa
     1141 cttctcctga agtattcgag atttatactg caccagaaga ttttgctgct gtacgagatg
     1201 agttggaaaa agagttcact ttggcgcaag cagaattgac gatgattcca caaacgacga
     1261 ttgatttaaa tgaggaacaa aaagagcaat tggaacgtct agtagataag ctagaagacg
     1321 atgatgatgt ttcagaagtc tttactgctg ctgaaatgta agtagcacat tgttagataa
     1381 taaaaagaaa gcacctgtac caatcgtacg ggtgctttct ttttatatat tatttttgtt
     1441 agaagaccaa tacaggcgtt cctttttcga ccatcccgaa taattccttc attacacttg
     1501 gtggggtgtt gatgcagccg tgtgaaccgc gggttttcca taagtctcca ccgtattcag
     1561 gttgccagtc tgaatcgtgg ataccaacac ctgtccagtc aatcggcatc cagtagttta
     1621 ccggactctc gtaaggtgtt ccatcgtcat ttgttccttt taaagttgca tcttcttctt
     1681 tattccaaac atagaagact ccagcaggtg taggtgtagt tggctttccg gaaacgatat
     1741 ctgtttcaag ggccactttt ccgtctttgt aataccacat atgctggttc tccagatcaa
     1801 cttcgatata cgtatcttcg atcaatggat gatcagcggt tgtgccacct tgcacaatgg
     1861 gtgaacgagt aaaatcttgg cctgccagaa tcgctttttt caatgcttct gtctcactgt
     1921 ctgtttggat cgtccaactg taagtgccaa ctgggaccgt aacttctcct ctttttgtac
     1981 ttttaaattt tgtgtcattc gtacttgtgt tatactttgt gcctaaatct gttacatatt
     2041 gacgtacttg ttctgtatct aaatcgactt ttccatcatt gtaggtcagc caactcataa
     2101 tatcacttga tgggatctgg aaagtctcac cgtttatact gtaagtagct tttacatttg
     2161 cgatagcatt catggatgcg agttgttctt tcagtgagct gtcttcagat gttacttttg
     2221 gtttttcttt gaattcagtc aattcaattg tatctttacc agagtttacc gcacttttta
     2281 attgtttgac cgcagcatcg acgtcgatcg tatttccatt gacttcaggc ttgataacga
     2341 aagagtcacc tgctttttcg atcgttgcat cagtagtagg tgtccggtct ttgttcaaat
     2401 cagtcagctt agttgtcaat gtctgaacgg tagtgtctag tttttggtgg ttttgactga
     2461 caggatctat ttcttgtttt tctgccgctg agacataagt cattccccaa gtccatccgt
     2521 tttgttcatt taggatcgga gaaagctccg aagtgaaatc atctttgtag ccgagctcgg
     2581 attttggtac ttcctgccag acttttccat cttctttaat agccagtttt ttatcagaat
     2641 aagcttcttt taatttttca tttgcttttt cgactgttag attgctgaca tttattccat
     2701 ttatttctgt attcggcaaa aaatgctttg tgtagtatgt actgcgatag gtataaaagc
     2761 tgatcagcac taagagaaca ccaagaactg aaagcaaagc aacatgtttc gatttttttc
     2821 gatgcgaaga tcttgtcata tcgacaactc cttaagtaaa agtttagggt ggaacggtct
     2881 catgatagca taatttagag gaaaagacga aggttatctc tactttatga aaaaatttag
     2941 aaaccataag ggtgataaaa taaaaaaacg gaaaacaccg aatagatgtt ttccgtcatg
     3001 ttcaaagatt attcagcgtc tagtttagca aaagcttcat ctagaatttc ttttaattgc
     3061 ttagcagaag cagccatacg gtcttgctcg ctgtcactta aaggaatttc gattactttt
     3121 tggatacctt gacggttgat aacagcaggt gcaccaatgt agatgtcgtt caatccatat
     3181 tcgccattca tgaatactga taaaggaaga acagcatttt catcatctaa gatggcacga
     3241 gtgatacgtg ctaatgctac tgcgatacca tagaaagtag cgcctttttt ctcgataatt
     3301 gtgtaagcag cgtcacgtac gccaaagaat aggttaacca tagcttcttc atcgatttct
     3361 gggttgtttt ttacccattc atagatttgc aagccggcaa catttgcatg tgaccaaact
     3421 gggaattctg aatctccgtg ttcacctaag atgtaagcat ggacgttacg tgcatcaacg
     3481 tcaactaatt ccgcaagtgc ttgacggaaa cgagctgagt caagagaggt acctgaaccg
     3541 attacgcgtt ctttagggaa acctgagaat ttccaagttg agtaagtcaa aatatctact
     3601 gggttagcgg caactaaaaa gataccgtta aatcctgatt caacgatttg tgtgacaaca
     3661 tcgcggttga tttttaggtt tttgtttact aagtctaaac gagtttcacc tggtttttgt
     3721 ggtgcgccag ctgtaataac aactaagtca gcatcatgtg cgtctgcgta agtagcagaa
     3781 tagatttttt taggtgaagt aaatgccaaa gcgtgagaca agtcaattgc gtctccttca
     3841 gttttagcag tattgatatc cacaatacct acttcttgag cgatattttg agtaactaga
     3901 gcaaaggcat agctagaacc tacggcgccg tctccaacaa gaataacttt ttgatgatct
     3961 tttttttctg cagctgcagt catttagtat caatccttcc attgatttgt tttttcccat
     4021 gcttatgcta acatttctca agccccatgt cacgctattt ggctataaaa tataacagat
     4081 aacaaggttg tattatatgt aaaagcttac atgtgcgtta tttcactatt tttatatatc
     4141 aattatttca ttaaactttg aagaaatttt taatttcttt atcatttatt aacgattctc
     4201 ttttagccga aacgccttgt tttgtgttat actatgcatt gacaagtgta aaaaagaaaa
     4261 aagctcgatg gactggaccg ctaattagca gtccagcctt ttgtgctgct ttttagaatt
     4321 aggtggaaaa caaaaatgaa aatgatcgtc gggttaggaa acccaggtac caaataccaa
     4381 tatacaaaac acaatatcgg ctttatggtc gtggataaaa ttgcacgcga gcatcaagct
     4441 actttcaaaa aaaatccgtt cgaagcagaa gttgccgaat tttttcataa tggagaaaag
     4501 atccttttag tcaaaccaca aacattcatg aatgaatctg gtcgtgcagt cggcccacta
     4561 atgacttatt ttgggattta tccagaggaa ttagtcgtaa tctacgatga tttggatctg
     4621 gcagtgggaa aaatccggct ccgtcaaaag ggaagtgccg gcgggcataa cggtatcaaa
     4681 agtattattt cccatttgaa tacgaatgtc tttgatcgaa tcaaggtggg aatcggcagg
     4741 ccagaaggga aaaaaacagt agtccagcat gtgcttagtc ctttcagcaa agaaaatcag
     4801 cctttgatag aagaaagcat gtgtcaatct gttaaagcag tcgaatatct catagaaggg
     4861 cattcgttcg tggatgcgat gaatcgattc aattgaggaa ggaggaaaag gaatggatat
     4921 tattcaatta gtcggaaaag atccgcaagt aaaagaatgg gtcagcaatt tagaagaaaa
     4981 aatgcctaga caactaatca caggattagc gggctcagcg aaaacacttc tatttgctcg
     5041 agcgtttaag aaaacaaata aaaacatgat tattctgatg ccaaatttgt attatgcgaa
     5101 tcagttagca gaagatctgc aacatgtgat tccgcaagat cagatccatt tatttccagt
     5161 ggatgaagtg ctttctgctg aaatggcctt ttcttctcca gaagcgcgag gcgagagagt
     5221 tgcaacgctt aacgcacttc aagaaaaaaa agcaggtatc tatttgttgc cagtcgcatc
     5281 tttgcacaaa cgactgccga ataagaaaac ttggctacag gcgcaattga attggcatat
     5341 tggtgatgag atagacgtgg aaaatcttcc tcgccaattg actttgatgg gctatgtacg
     5401 agaagagatg gtcgcaaaac cgggagaatt cagtattcga ggaagcatta tcgatattta
     5461 cccgttaacg acaacctatc ctgttcgtgt agaactattt gatgtagaga tagattctat
     5521 gcggtatttt gaagcagaaa cacaacgctc aatcgaaaaa acggaagaaa tctgggtttc
     5581 cccaacgagt gaacaagtct acggaccaga ggatctagaa catggaatag agcaaatgca
     5641 aaaaatgcta gaaaaacggc ttgctgcgac cacagaagaa gcagaccgcg agtttttgca
     5701 agattacttc ggtcagctga cttcaagctg gcaagcaggt gtcccgacag aagaagcaaa
     5761 attctactca gatcttcttt accaagaatc tgtgacgatc ttggattatt tcccttcaaa
     5821 tagtttacta gtcgttgatg attatcagcg aatcatggaa acaaatcggg aaatcgaaag
     5881 ggaagcggca gaatggcaga ctcaaaaaat cagcgaattg cgggtgtttt ctgagcagac
     5941 attttcagca gatatacaaa aaatcgtgca gaaggaaaag ttcaccacaa cctttttctc
     6001 actcttccaa aaagggatgg gaaatcttcg tttccaagca atccataatt ttcaatatcg
     6061 gacgatgcaa cagttcttcg gacaaatgcc attgctgaaa acagaaatgg accgttggcg
     6121 caagcaaaaa cagactgttg tgatctttat tccgacaaag gacagaatac gcaaagcaga
     6181 acaaatgctt cgagatgaag acatattagt agtagaaaca gaaaaaaatg cattgatcag
     6241 aggacaggta caattagttg aaggagcttt gcaggctggt tttgaacttc ctcaagaaaa
     6301 aatagtagct atcacagaaa aagaaatttt ccaaaaaacg acgaaaaaac aaacgagacg
     6361 tcaaacagta acaaacgcag agcgattgaa aagctataat gagttaaaag caggagatta
     6421 cgttgtccat gccaatcatg gtatcgggaa atacattggg atggaaacgc tagaagtaga
     6481 tggcgttcac caagactata tgactatttt gtatcaaaac gacgacaaac tgtttattcc
     6541 tgttactcaa ctgaatctga tccaaaaata tgtcgcctct gaagccaaag cgcctcgcat
     6601 caataagcta ggcggaagtg aatggacgaa aaccaagcga aaagtctctt caaaaatcga
     6661 ggatatcgct gacgatctaa tcaaattata tgccgcaaga gagtcagaaa aaggatatgc
     6721 ttttggacct gatgacgcct accaaaaaga attcgaaaat gccttcccct actctgaaac
     6781 tgatgaccag ttgagaagtg cagcggagat caaacgggat atggaaaaag aaaaaccaat
     6841 ggatcgacta ctcgtaggtg atgttggtta tgggaaaaca gaagtagcgc ttcgagcggc
     6901 ctttaaagcg ataaaggaaa gcaaacaagt agccttttta gttccaacaa cgatcttggc
     6961 acagcagcac tatgaaacga tgctggaaag atttgaaggt ttccccgtaa atatcgggct
     7021 attaagtcgt ttcagaacaa gaaagcaaca aaaagaaacc atcgaacaac tgcgtacagg
     7081 tcaagttgat attgttgtag gaacccaccg aattttgtca aaagatattg aatttagcga
     7141 tttgggtcta ctgatcattg acgaagaaca acgttttggt gtaaaacata aggaacgatt
     7201 gaaacagtta cgtgcacaag tggatgtttt gactctgact gcaacaccga ttccgagaac
     7261 tttgcatatg tcgatgttag gcgtgcggga tctttcagta atcgaaacac cgcctgcgaa
     7321 tcgttatccg atccaaacgt atgttatgga aaaaaatcct ggggctattc gcgaagcaat
     7381 ccatcgagaa atgggacgag gtggacaagt attctattta tataatcggg tagaaacgat
     7441 cgaacaaaaa gtagaagaaa tccaagaact cgttccggaa gcacggatcg gttatgctca
     7501 tgggcaaatg acagaggcgc aattagaaaa tacgcttttt gaatttattg aaggtcaata
     7561 tgatgttctt gtcactacaa ccatcattga aacaggagta gatatcccga acgcaaatac
     7621 gttgttcgtt gagaacgctg attatatggg attatccacg ttataccaat tgcgagggcg
     7681 ggtaggaaga agcagccgtg tcgcatatgc ttactttatg tatgagcagc agaagatcct
     7741 taacgaagtc agtgaaaaac gtcttcaggc aatcaaagat tttacagaac tgggatctgg
     7801 attcaagatt gcgatgcggg atctttccat tcgcggtgca ggtaatctct taggtgctca
     7861 gcagcatgga tttattgatg cagtcggttt cgatatgtat tctcaaatgc taagcgaagc
     7921 tgtttctcgt aaacaaggaa agaatagtca agtggagaag accactgttg agatcgacct
     7981 cggtgtcgat gcgtatctac ctgaaacata cgtggcagat caacggcaga aaatcgagat
     8041 ctataaacga attcgtgaac ttgattcgca agaaatgcta gatgaactag aagatgatct
     8101 gctcgaccgt tttggagaat atccagaaga agtagcacat ctcttgacaa ttgggcagat
     8161 caaaatggat ggcgatcgag cgctcctaga aacgataagg aaacaggagg cgcatatagt
     8221 ctttactcta agtaaagttg gaacaaaacg ctatagtgtg gaacagttat tcgaagcact
     8281 gacagcgaca aaactaaaag ccagtctagg cgttgaaaaa gaacagatgg tgatacgctt
     8341 gacgatcccc aaccaaatgg aagaagcagt atggcttcag gaaatccaaa agtttgtcaa
     8401 agcgttgcgt gagcaaaaat atctgtcagc gtaggaagga gcaatgattt ggatcataaa
     8461 gaaatgcggc gcgtcatgca aggagcattt gtattgacga ttgcttcttt catagcaaaa
     8521 gtattaagtg ccctttaccg aattcccctc caaaacttag tcggcgacga aggtttttat
     8581 gtttatcagc aagtttatcc aatttatggg atagcaatga ctttagcttt atccggtctt
     8641 ccgcagttca tatcaaagta cgttgcagaa agaaagaatc ctttcgagcg aaagcaagct
     8701 ttgcaaaatc tttatccctt agtattttgg acaggtgtct ttttgtggtt gtttgtcttt
     8761 gcatttagca gctggattgc ttggatgatg ggagacatac aattgaaacc gctgatccaa
     8821 gtagtttcct ttaccttttt gctgatgcct ggtctatctt tttatcgagg gaatttccaa
     8881 ggaagattct tgatggaacc aactgctgtt tctcaagtag ttgagcagtt cgttcgtgtt
     8941 tttgtgattg tctgtagtgc gtttgctttt agaaagcttg gatggacggt gtaccaaaca
     9001 gggacggcag caatgagtgg agctgttttt ggcggtttat gcgcatatgg gatccttcac
     9061 tactatgaac aaaagattca tggaggagcg ttaagcttcc gacggtttcc attgttcaaa
     9121 cagccgtcaa agcccttgat ccggcgtttt ctggtagaag gcggactggt ttcgatttat
     9181 agtggattgc ttatcttttt ccagctgatc gactctttct ttgtaaaaaa tgctttggaa
     9241 gtttacggac tgtcagaata cggtgcgaag atagcaaaag gggtctatga tcgaggtcag
     9301 cctttagtac aattaggttt agtgattgcc actgcattaa gtgcgacatt cctgcctgcg
     9361 ttgactcgtc atttgaccaa ccggatctat cgtcagtttt tacagacagc caaaatctat
     9421 cttagattga cgaccgcttt agcattagct gcgtctttgg gattagcctt gctgctccct
     9481 tatatcaatt atgcgttgtt taaagattat gcgggaaatg cagcgcttgt attatttgtt
     9541 ttttccattg ctttcactgc agtcatccaa gcttatcaaa gtattgctca aagcaagaac
     9601 tcgtttcgcc cttctttaaa aggcgcagga tggggactat tggtaaaagg attgactact
     9661 tcttttctga ccggtttatt aggaacagca ggtgcaagcc tgtctaccct tttaggatta
     9721 ggtgtgacgc tttggtattt tgttcgaatt gaaacaaaag agctaaatgc tttttggaaa
     9781 gaacggcatt ttggacggaa gttgatgttc agccttcttt gtatgacagt agtactgctg
     9841 ctgtattatg gtgtattgaa tatagtattt ggtcctgtaa ctcatcgttc cactgctttt
     9901 tttgtcagcg tgataggagt tgggatcggg gcttctgtgt tcatttatgc agtgattcgt
     9961 cttcgtctgt ttacagtacg agaatggcta atgttgcctt ttggtaagaa aatcctgcga
    10021 ttaaataaaa aaattaa
//
LOCUS       DARDHA010000098        10030 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000098 DARDHA010000000
VERSION     DARDHA010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.099"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(494..718)
                     /locus_tag="SDA10_001932"
     CDS             complement(494..718)
                     /locus_tag="SDA10_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240826.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            909..3686
                     /locus_tag="SDA10_001933"
     CDS             909..3686
                     /locus_tag="SDA10_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG4240827.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3767..4186
                     /locus_tag="SDA10_001934"
     CDS             3767..4186
                     /locus_tag="SDA10_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEG4240828.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4198..4668
                     /locus_tag="SDA10_001935"
     CDS             4198..4668
                     /locus_tag="SDA10_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4240829.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4685..5464
                     /locus_tag="SDA10_001936"
     CDS             4685..5464
                     /locus_tag="SDA10_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4240830.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5451..6272
                     /locus_tag="SDA10_001937"
     CDS             5451..6272
                     /locus_tag="SDA10_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG4240831.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6289..7338
                     /locus_tag="SDA10_001938"
     CDS             6289..7338
                     /locus_tag="SDA10_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG4240832.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7351..8355
                     /locus_tag="SDA10_001939"
     CDS             7351..8355
                     /locus_tag="SDA10_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG4240833.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8988..10007
                     /locus_tag="SDA10_001940"
     CDS             8988..10007
                     /locus_tag="SDA10_001940"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEG4240834.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3362 a         1652 c         2094 g         2922 t
ORIGIN      
        1 ttctttttta atttgttctt gcaatctttt ttgctacgta atttttgtaa aaaagtgggt
       61 tgagagaaat ctaattgatt ttcaaagaag gagtatgaaa atgagaaaaa tatttattcg
      121 tttattgtgg gtactatctt agaggaataa ataatagtgg agaaaactat ggtttggcgc
      181 agattctatc aggtttgatg gaacgaagac aacctcccga catcaagtca aaagctactt
      241 gagattttga tctgaaaata aaaaaagaaa acaaggtagg aacatcaatg tttctacctt
      301 gttttcgtaa tcaatgggaa aaaactaaga gtggtgtacg taatcaataa aaaggatggt
      361 gacaacagta cctctttact ctctgaaagc atcataactc gtttttcctc attatgccaa
      421 taaattgttg ttataccaca tataaggatt tgtaatagct aaaaacagac aggaaagttg
      481 aaatctgtga atcttatttt gtatggaagg atttatagaa cgcaaaaatc aagataaaga
      541 tgaataaaaa tcctgtttcg gctatatatt gcgtatctac gccaataaaa cgagtttgtt
      601 ctgtcatagc catataaagc aaaggcaaaa atacaactac agcgagataa gatccgatga
      661 tttctcgaaa agttttccag gaagtatgtt tcctgttttt atatttatcc gatttcattg
      721 aatagtcata gcctccttat tctttcatat gttgaaattc cagtataaaa actggaagga
      781 actgtacgtt ttttagaata aaaattataa gggataagta ggctgctgtc tataataaga
      841 tatatttttc aattctttat taaagatttt attgacaaaa tgaatccgtt ttctatgatg
      901 gaggaggggt gaataagttg ttgaagaagg aaattttatg ttacttagag aaccagacag
      961 cctttttcga tttagaacat atgaacgagg tctttacagc acaaaggtta gctgatcatt
     1021 ttggggtaaa aagaaacaca gtcagtcatt atttgaacca gttaaatgat gagcaactac
     1081 tagtcaaaat caattctcga ccagtgattt atttccacaa agaagctttt gaaaaacaat
     1141 tttttaagtt acgcacgaat ttttattttt ctatcgatga tctcaaagag gaacagcctt
     1201 ttttcgcgca accaaaagac ttattctctt ttatgatcgg atacgacgcc agtttgaaag
     1261 aaagtatcga acaaatcaaa acagctcttt actatccaga tggcggatta ccacttttga
     1321 ttacaggcga aagtggcaca ggtaaaagtt atttagttaa tttggtctat cagtattgtc
     1381 tgcttcatga tctgctagaa gattctgcac cattcatcac ttttaattgt gcacaatatg
     1441 cagataatcc tgaacttttg actagcaatt tgtttggaca tgttaaggga gcgtacactg
     1501 gtgcagaaga aaataaaaaa ggggcttttg aagcagcaga tggtgggatc ttgtttttag
     1561 acgaggtaca tcgcttaaca ccagaaggtc aggaaaaact gttcacctat cttgatcaag
     1621 gtgtgattta tcgtatggga gaacctaatt tgtcacgccg gatcaaaaca cgtctctttt
     1681 ttgctacgac tgaagagatt actagtcatt tcttaacaac tttcattcga agaataccta
     1741 tccgaatcga actacctgca cttaatcagc gtagtcgtaa tgaacggcta gaactgattt
     1801 actctttttt tctcgaagaa caaagaaaaa tggggagccc tttgtcagtc agtggaccag
     1861 ctttgtcact tttaacaagt cagaaattat ccggaaacat aggagagctg aaaaatatcg
     1921 tcaaagtttc agcagcaaaa gcatatgctg aacagcgtga acagcctgaa atccatgtca
     1981 cgatccatca tttgcaaaaa gaactacttg cgcagcctat tactaaaaat acagcacagc
     2041 aaggaatcta tattacgcaa gaccagacct tagagcaatt aatggagaag caacagcccg
     2101 aacagcagcg aatcgttaaa agttttgagc aaatcctaat tgatttcaag aaaaataact
     2161 gtctgctata ttcttgcgaa ggaaaattaa agcaagaagt gattcaatta tttgattttt
     2221 tgctgtttga gaccagtaga caagaaaaac acgagctgct ggtttattgg acacaatcta
     2281 tccgagagac ctttcgacaa atggaaactg cctatcaaat caaattcgat ggaaacagtg
     2341 tctatgcctt gagttactat ctctatcagc gaagcactat caaatggtta ccggaagaac
     2401 atgatattat ccaactaatc aaagaattag aacaacaagt ttctcagtct tatccatcta
     2461 gttatcacta tgtccaacga attctggaat taagtaagat caaattagat attgaagaca
     2521 caagtatgga tcggatttta ctgacgatct atctaaaaaa agcgaaatgg tcaaaagaaa
     2581 aacgactgcc aaaagcgatg attgctgctc acggttatgc aactgctagc agtatggcaa
     2641 atgtagtaaa tcgtttatta caagaggatc tatttgaatc atttgatatg ccactagatg
     2701 tcacaccaca gcagattgct tcagaaatcc ttgattattg cgaaaacagc gatgtttcaa
     2761 atggattgat catattagta gacatgggat ctctgaaaga aatccatcag ttttttaaaa
     2821 aacagttatc cgtaccgctt ttgattttga ataatgtgac gacaccttta gcaatcacag
     2881 taggggaatg cctgcaaaaa aatgtatcat tagaagagat agcagaagag actgttcgac
     2941 agatccagcc agaatggcga ctgctctatc cagaggaaaa caaacccaaa gcactcatta
     3001 ctacttgctt cactggaatc ggtacggcgg tccacttaag tgaactgcta gaaaaaagct
     3061 tacctacggc ttgtcagttg aaaatcatcc catatgaata tcagcaatta aaagataaga
     3121 aaaacagtga cccattattt tccatatacg aagtcgtagg aatgattggc accacagatc
     3181 ctgatatcac caatatcgac tatctttcat tagaagacct catctcgggg gaaaagatgt
     3241 ctgtactagc tgaatggttg gacactacta tgaatgcttc agagaaggaa atattcaatc
     3301 agcgcatcat tcgtaatttt tcattggaaa aagtactgga ttccgtgact attctagata
     3361 cagagaaggt gatgggagaa atcgatcatt ttatgagaga attagaggtg cagttgaatc
     3421 gaagcatcag caatcccaaa aaattagcgt tgtatgtcca tgtgagctgt ctcattgaac
     3481 gattgatccg acaaatgcca atcgaaacct atcaaggatt agagaaatgg aagcagtgtc
     3541 aaaaagaagg gttatctgct attaaatctg cgtttagtgt cattgaagag cattatagtg
     3601 tcatgatccc caattctgag attgcctata tctatgatat tttgagtgaa gatacagagt
     3661 ttctctcgat agaagaagaa ttttgaacat aagcaagttg ttgccactaa ttttggcatg
     3721 caacttgctt ttttagtatg tgtaagtgaa aagaggagat gaaaaagtgg atagagaaat
     3781 tatcttagct tcccatggaa aatttgcttc agggatatta gattcattag agctgatcta
     3841 tgggaaaaat cattctatta ctgtattcga ttgctatact gatgagaatt ttgatttagc
     3901 ggaaacagta aggcagcttt ttatgaggta caaagataaa gaaatcattg ttctgacgga
     3961 tctttttggc ggaagtgtca acaacgaatt cttacaatat atcaatcggg atcatgtcta
     4021 tctcgttgcc ggattgaatt tgccactatt gattgagcta gtatctcggt tggacgctga
     4081 agtagagaca actgttttga tccagcaggt gctaactgat tcaaaaaaaa tgatccagtt
     4141 ttgtaacgag cggctcaatc agaaaataga agaagacgaa ttttaggagg aagaaaaatg
     4201 attgtattga caagagtcga ccatcggtta ttacacggac aagtagcttt ttcatggaca
     4261 caaacaatag gcgcagactg tattttgatt gctaatgatg atgtacctac gaatgagata
     4321 cgaaagacaa cgatcaaatt agccaagcca caaggagtca aactagtgat caaatcaatt
     4381 gatgactcga ttgccgcgtt gaaaagcggg gtgacagata aatataagtt attcatcgta
     4441 gtggaatcga tcgaggatgc atataagctg gcagaagcgt atccagaaat caaaaccatc
     4501 aacttaggag ggatcaaggc aaaacctggt actcgcagca tcggaaaagc ggtaaatatt
     4561 ttaccagaag aggaggaacg cttgaaacga ttaattgaca aacaaataga agtagaaatc
     4621 cgtcaagtac cagcagataa aaaagtggat gtaagagaag ttttgtaaga aaggaagaac
     4681 ctcaatgatt gtacaagcag tcttattagg aattgttgca tttgtcgcac aatcagaata
     4741 tgcactaggg acatcgttgc tttctcgtcc gattgtgacg gggctgttta caggaatcgt
     4801 gttaggtgat gtaaaaactg ggatcattat gggcgcaact ttagagttag catttatcgg
     4861 atcgttctca gtaggtgctt cgattccacc tgatgttgtg accggtggga ttttagggac
     4921 ggcttttgct attacagctg gagccggtac agaaaccgcg cttttactag gtttgccgat
     4981 tgctacgttg acattgatat tgaaaaatat ttacttaggg cttttgattc caattatgaa
     5041 ccataaagca gacaattatg cagaagaagg aaattataaa ggagtcgagc atatgcactt
     5101 gcttgccggt atcggtcttt ccttgatgct tggtgttgtg gtaatggttt cttatatggt
     5161 gggaagcaat acgatcggaa atctattaaa tatgattcct gacttcgttc aaaaggggtt
     5221 gtcagtagct acagggttga ttccagctct tggttttgcg atgcttgctc gtttgctgat
     5281 caacaaacaa gttgcaccat attttttctt aggttttgct atagcttctt acttagagat
     5341 tcctgtgacc ggagtagcta tttttggtgc gattttagca gttgtggtag tcaatattat
     5401 gaatgtccgt caactaccac ttcaaacaac tgcaggggag gtagaagaag atgaagaatt
     5461 ctgagatcaa aaacagcaaa ataacgaaaa aagagctaaa tcacgtattt tggcgttctt
     5521 ttcaaatgga gttttcttgg aattatgaac gtcagatgaa tatggcttat gtttatgcaa
     5581 tgattccgat tttgaaaaag ttatatcagt cgaaagaaga aatggcagca gctttgaaac
     5641 ggcatctaga gttttttaac acgaccccac atatcgttac actgatttta ggaatcacgg
     5701 ctgcaatgga ggaagaaaat aaggatgatc ctgaatttga tgtgactgcg atcgatagta
     5761 tcaaaacatc tttgatggga cctcttgcag ggttaggaga ttctttcttt tggggaactt
     5821 tgcgattgat tgccacaggt gtgggtacct ctttagccat gcaaggaaat attttaggcc
     5881 ctatcttgtt cattttgatt ttcaatattc cgcacattct gtttcgctat ctagcaacag
     5941 gttggggata tcgcttagga acgggttttt tgaaaaagat ccaggcaaac ggtatgatgg
     6001 aaagtttgac attaggcgcg tctattattg gactgatggt cgttggagca atgactgcca
     6061 cgatgatcac catcaatatt cctttaaaaa tcggttcagg agaaaatgct gtgactgttc
     6121 aaagtatttt tgatgatatc gttccaaata tcttatgtct tggagcattt ggcatagtgt
     6181 tttatctatt gaagaaagaa gtgaaaccat taacgatctt gatcggacta gctttctttg
     6241 gaatttttgg ttcgtggata gggatttttt aaaaaaggag cagagaaaat gtttacgatg
     6301 caagattata tctatgaaga aaaggaagta ctaagtacga tcttgaaaaa gaatgatttt
     6361 tcgacaagag aacatatgaa aaaaacagtg aatttactga tcttagcaac gggttcatct
     6421 tataacgctt gtttagcagc gaagccagca ttggaatctt atggtgatct tactgtcgat
     6481 attcaagaac ctttttattt tttcaattat ggaaaacttt ctccttcaat cgatactgtg
     6541 attgctgtgt cacaaagcgg gaaaagtgct tctactgttg aagcagtaaa aatgatacaa
     6601 aaacaagggt tacctgttgt tgctatcaca aatgacgtgc aaagtccact tgctttagaa
     6661 gctgcacaaa ttattgattt aggtgccggg gtagaatcag taggcttcgt tacaaaaggc
     6721 tattctgcga ctgttttaca gttactactc ttagggatag gaattggaat aagtaaaaat
     6781 aaaatcagca aaaaaataga acaagactac atgcaacagt tgcgcaaaat aatcaaccat
     6841 ttaccagcga ttattcaaaa aacggaagaa ttttttgatg gatatcaaag tttatttcgt
     6901 ttagcacagc gtttcgtcac cattggatat ggtccaaact ggggaacagc taaagaagca
     6961 gagacgaaat tgactgaaac aatccgcgtt ccgtcacaag gatttgagtt agaagcttat
     7021 atgcacggcc catacctgga ggcagatgct tcgcatttgc tattctttat tgaaggggaa
     7081 agtgtaaata aggaacgttc gcaaaagttg caaagatata tgagtcggta cgttggagag
     7141 actctcacca ttaccacaaa aaaagcaaga aacgagaaaa cacttggatt agcaatagaa
     7201 tgtgatgaat atctgtctgt attagcgctt gtcgttccgt ttcaactatt cgcttataaa
     7261 accgcagtag ctaaaggtat tgacttgaac aaaaagattt ttgaagactt tgatacagtc
     7321 ttgaaaagta aattataaga ggtggaaatc atgctgaaat tcaatgaaga agaacaaatc
     7381 aaagcaataa aaggcgcact agctttacgt ccgcaagtag aagaaatcat cgataaactt
     7441 tatgcggaaa aatttgacgc tgtctattat ctaggaatcg gtggaacgta tgcttcctcg
     7501 atgcaagctg tcacttatat gaatgggaaa agcaacttgc ctgtctttgt acagcatgct
     7561 gcagaatatt acactactgg aaacaaaaga ttaacaaaag attctatcgt tattttgtcc
     7621 tctgttaccg gtacaactca agaagtagtg aaagcagtag aacaaatcaa gaaagtagga
     7681 gcgacactga ttggatttat cgataaagcg aacagtaagt tgtctcagct atgcgatttt
     7741 gtggtcactt atccagctcc tggaacagaa caaatcaaat tttttatggc agcagatcga
     7801 ttgatgtatt tgaatggaga attcgaagct tattcagaat actatgaaca gctcgaacgt
     7861 tatttaccaa cagggttagt ggaagcagag aaaaaagcag atgcatttgg cttatcattt
     7921 gcagaaaaac atcgacgtga tagtatgcat tattttattg gagcagggaa tcaatgggga
     7981 gctgtttatt catatgccat gtgttactgg gaagaacaaa gctggcttcc atcgaaatct
     8041 atccatgctg ccgagtttct tcacgggacg ttagagatcg tagaagaaac aaccccagtc
     8101 acattatttt taggcgaaga tgaacagaga gtgttatctg aacgtgtggc taaacttttg
     8161 ccaaaaattt gcagcaatta tacattgata gacacaaaag actatccagt agaaggtatc
     8221 agtgaaaaat atcgcggacg tgttttgtct ttcttattga tgcatgtagt gacacaacgc
     8281 atcgatgctc atgtggagca gttgaactgc catccattag agatacgccg ctactatcgt
     8341 cagtttgatt attaggatag actggataaa aagaatataa aaaataaaga gagcagcgat
     8401 aactgctccc tttatttttt taaaatattg gcatcaatta cgttataaaa cacatttttt
     8461 gcattatcta cagttcaact tataatctgt ttaatgggac atgtgggtgc caaccatttt
     8521 ttgttatgca atagtactat ttagttgttg catggatccg tagtaaaagt ccaagtacaa
     8581 cgagttgcgc cagtttacat agcgttttgt atccagaatg ttgactaaaa caaaagttcc
     8641 tatgtcaacc gtcaacagag acaatctgtc cgttgcagga cctgacagat ttagaaatgt
     8701 tcaatctgtc tcttatgtat tttaaatcaa tactcactag gagaagtttc ctaacctcct
     8761 aatagtaatg ctggtagtaa cattgtcaaa actgcttcct cttaggaaca agcaaagcat
     8821 aacgagtgta aaaataaaat gtagactatt gtaacatatc attgattatt tcaagtaaaa
     8881 acgataaaag aagtaaacaa acacttaaaa acaatggaaa ttagtcccaa ctttaagaaa
     8941 tgtatactat aatggaaatt atcaattatt tatttggagg aatatccatg aaacaaaaaa
     9001 tcgcagttct tggtcctgga tcttggggca ctgctttagc acaaacattg gcagaaaacg
     9061 ggcatgatgt ccgtatttgg ggaaatgtac ctgaacagat tgatgaaatc aataagtatc
     9121 atacaaatca gcatttttta cctgatttga cgatcccaga atcgattatc ggatataaag
     9181 aattggcaga agcagtggat aacgcagatg ctattttatt tgttgttcca acaaaagcga
     9241 ttcgttcagt cgcacaggaa cttattggta aaatgaatac taagccaatc attatacatg
     9301 caagtaaagg actagaacaa gatacgcata aacgaatctc agaagttttg gctgaagaaa
     9361 ttccagaaga aaaaagacag gcaattgttg tattatctgg tcctagccat gctgaggaag
     9421 tggcagtaca tgatatcaca acaattacag ctgcaagtat tgatcaaaaa gcagcagcct
     9481 atgtacaaaa actgttcatg aatgattatt tcaggattta tacaaataat gatgtgattg
     9541 gcgtagaaac tggtgcagca ctgaaaaata tcattgcaat tggtgccggc gctatccatg
     9601 gattaggatt cggagatgat gcaaaagcag caatcatgac tcgaggattg gcagaaatca
     9661 gccgtcttgg tgtcgcgatg ggtgccaatc ctctgacatt tatcggtttg agtggtgtgg
     9721 gggacttgat cgttacttgt acaagtgttc attctcgcaa ttggcgtgcc ggtaacttgt
     9781 taggacaggg acataagctg gaagaagttc tagaaaacat gggaatggtc gttgagggtg
     9841 tggcaacgac aaaagcagca gtagagctag ctcaacagct aggtgttgag atgccaatta
     9901 ctcaaacaat ctataatgtt ttgtataatg gagaagatat caaaaaagca gcaaaagaaa
     9961 tcatgttacg tgacggaaaa atggagaatg aattttctct tcgttaaata gatgatcaat
    10021 agttaatcgt
//
LOCUS       DARDHA010000099         9908 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000099 DARDHA010000000
VERSION     DARDHA010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.100"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            91..3318
                     /gene="fss3"
                     /locus_tag="SDA10_001941"
     CDS             91..3318
                     /gene="fss3"
                     /locus_tag="SDA10_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEG4240835.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            3431..3745
                     /locus_tag="SDA10_001942"
     CDS             3431..3745
                     /locus_tag="SDA10_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG4240836.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            3758..4132
                     /locus_tag="SDA10_001943"
     CDS             3758..4132
                     /locus_tag="SDA10_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG4240837.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            4142..4477
                     /locus_tag="SDA10_001944"
     CDS             4142..4477
                     /locus_tag="SDA10_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HEG4240838.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            4560..5900
                     /locus_tag="SDA10_001945"
     CDS             4560..5900
                     /locus_tag="SDA10_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG4240839.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            5978..6652
                     /locus_tag="SDA10_001946"
     CDS             5978..6652
                     /locus_tag="SDA10_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEG4240840.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            6645..6809
                     /locus_tag="SDA10_001947"
     CDS             6645..6809
                     /locus_tag="SDA10_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240841.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            6885..8027
                     /gene="dcm"
                     /locus_tag="SDA10_001948"
                     /pseudo
     CDS             6885..8027
                     /gene="dcm"
                     /locus_tag="SDA10_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            8017..8307
                     /locus_tag="SDA10_001949"
     CDS             8017..8307
                     /locus_tag="SDA10_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240842.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            8566..9750
                     /locus_tag="SDA10_001950"
     CDS             8566..9750
                     /locus_tag="SDA10_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG4240843.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            9747..9884
                     /locus_tag="SDA10_001951"
     CDS             9747..9884
                     /locus_tag="SDA10_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEG4240844.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         3282 a         1670 c         2245 g         2711 t
ORIGIN      
        1 gtcaataaaa attttaaaaa gactttataa acaaagatgt tcaagcgagc atcttatttt
       61 tttgtgctta atttgaaagg agaacatttc atgtataaaa aacaaaaaaa gaaattatgt
      121 gtattaacta ttttgagttt ggctgttcaa gcaattgttg gagcaattgc tcctacgatt
      181 acttttgctg atgaaattac ccatccacaa acggtaaccg tagaactaga tttagcacat
      241 caatatgctg ttgaaggaac ttttagtgat ggtcgtccaa tgtcagaagt cactgttccg
      301 cattatgcag tgtacaacgg ctttaaacaa gatattttct gtattgaacc aggagtcccg
      361 atttacaatg agttcactcc tggctatgag aaaaacccgt taccagatat gtccgataaa
      421 gccaaattag tttccgtttt atggaaaaaa gtaggtacag atgcagatac tcatattgtg
      481 gcacagaaga tgatctggca agaagttaat gggtataccc ttcactcaat aaaacgatca
      541 gatggtagtg ccgtaaatat cgcagccatt gaggcaaaaa taaatcaagc cattgacgat
      601 tatcagaaga aaccaacctt ccataatagt acggctaaaa cagtattagg gcaatcgact
      661 actgtgacgg atacgaataa tctgaatttg tcagagttcg atgaggttgt ggaaaatgcc
      721 gcaaacattg attatcgtgt gaatggtaat cagttgataa tcaccccaaa tgccaattca
      781 aaagaaagtg gtgtgctgac gcttaagaaa tctgctggta ccggaacacc tgtcgcctat
      841 aaaaaggctg gccaacagac tttaatggcc ggggcgattg ataaacctaa tacctacaca
      901 atcaaaattg atgtggaaac ggagggttct ttaaagatca aaaaagtcga taaagaatcg
      961 ggtgatgttg taccaggaac ggttttccat ttagactttg gaaaaacttt acctgcaaaa
     1021 gacgtgacta ctgacaaaga aggcattgct acattggatg agattcctca tggtacaaaa
     1081 gtaaccatta ccgaaaaatc agtaccggcg ccttatacga ttgataccac ccccatgact
     1141 accaccatta aagcaggcga aaccatttac gtgacttcaa aaaacgcacg ggaaaaaggg
     1201 caaatcattc tggataaaag cggagttgaa acagggagtg atctttggaa tgacaattat
     1261 tccttagctg gtaatacgtt tgccattcgc aaagacagtc caaccggtga aatcgttcaa
     1321 gaaatgacca cggatgaaaa cggtcatgcg gaaacaccaa aagagattgc taatgcgttg
     1381 gaattgggaa cctattatgt gaccgaaact aaagctagtc atgggttcgt gaatacgttc
     1441 aaacctgtaa aagtcgaatt gaagtatgcc aatcaaaccg tagcccctgt taccagtaac
     1501 gtaaaagggc aaaatcaaga agtcactggc gaaaccactt tgacgaaaga agacaaagat
     1561 actggggata aagcacaagg taaggcagtc tttgagggtg ctgaatacac cttgtttact
     1621 gctaaggatg gaaaagctgt taaatggagt gaggctttta aaccagagtt agcgaaggga
     1681 acgaaaggtt ctgatgaaac ggtcactttg actttagacg aaaagaacca agttgccgtt
     1741 aaacatttag ctatcaacga gtattattgg caagaaacaa aagcgcccga aggatatagc
     1801 cttgatgaaa caaagtatcc tgtatccatc aaaaaggtgg atgatagcga aaaaaatgcc
     1861 gtgattaccc gaaatgttac ggcaaaagaa caagtgattc gctttggctt tgatttcttt
     1921 aaatttgctg gatcagctgc gggcactacc gaaactggct tcaatgatct gacctttaaa
     1981 gtgtcgccat tggaagggac caatgaaatc acaggtgccg aagatgaagc gaccacagct
     2041 tataacgagc aattagggtt tgatggttat ggcaagtttg aaaatcttcc ttatggggat
     2101 tatttacttg aagaagtaga agccccagaa ggttttcaaa agattacgcc attagaaatc
     2161 cgttctactt ttaaggaaaa caaagaagac tttgtgaaga gtgagtatgt ctttaccatt
     2221 accgaacaag accaaaaaca accaattaaa acggtgaccg ttccttacga gaagctgacg
     2281 aacaaagcat tttctgttag tttgaaccgt ttaatgctct atgatttgcc tgaggaagaa
     2341 gatagtttaa cttctcttgc gacttggaaa gacggaaaca aagaattaac ttctctggat
     2401 tctaccgagc tagttgataa attgagttac aatctacatg aaatcaaaga agactggtat
     2461 gtggtagcgc aagccattga tgtggatgcg acaaaagctg cccaagaaaa agatgaaaaa
     2521 gccaaaccag tggtgattgc agaaacaagc gcaacgttgg cgaacaaaga gaaaacagga
     2581 acttggaaaa ttcagcataa attaaccgcc gaacaagttt taaataaaac tattgtctta
     2641 ttcaactatg tgtatgaaaa taaggaagct tttgaagcag gcgataagcc agtagcgaag
     2701 gatgttagct tgaataacca agcccaaact gtcagttgta cagtggaaca ccatgtttct
     2761 atccaaacaa aggcccacct agaaaacggt tctcagacct ttactcatgg tgatgtggtg
     2821 gatatgtttg atgatgtgtc gattactcat gatgtactgg atggctcaaa agaagctttc
     2881 gaaacaattc tgtatgcttt attaccggat ggtacaaaca aagaaatttg gaaatctggc
     2941 aagattgatt atgaagtaaa tgataaagaa ttcaccaaaa ctgtgcttgc gaaaaaagta
     3001 gataccggaa aatatccaga aggaaccaag tttacttttg cggaaatcaa ttacgataaa
     3061 gatgggaata tcaatggaaa acacaatgaa gatttgaaag aaaaatctca aaccttaaca
     3121 ccaaaagaag ttccaaccat actaagtacg ccgaaacaac cggaaacacc aactgttcca
     3181 agtggctctc aagaatctag tcccacagtg aagaccttcc ctcaaaccgg ggagaaaaat
     3241 tccaatgttt tactgttcat tggttttact ttgatttttg cgacggcggg ctattatttc
     3301 tggaatcgcc ggaactaagg tgatgagatg atacaagtaa atcctaagaa ccaacgtgcg
     3361 cgaccgcctc ctaaaaaagt agtcgcgcta tttttgtatc caaaactaac aatgaaaaga
     3421 ggaaaagaag atggaattga aatttgtcgt gccagatatg gctgaaacct ttggaaagtt
     3481 aagttatgcc ggtgaaggag aaattataac agaaggatat ggacggaata ctacggtgat
     3541 tggtcgcagt tatcatttgt attccagtaa gcaacgggcc gatgatattg aggtggtagt
     3601 agctgcggaa gccggtgaaa aggattttga tcaagaccaa ccgctaaaag ccgtgaatcc
     3661 ccatttggtt gccaaaggct atgagattga gaatcgtggg tttactgatt acgtgttgta
     3721 tgttgatgat ttagtgaaag cataggagga aaagaagatg agattaacag aaggtattgt
     3781 cgtagattca ggtttaacat ttgggaagtt gcgtttttcc gcattacgcc gagaagtacg
     3841 gaagcaaaat gaggatggaa caattggtaa tgaggtaaaa gaacgaacct ataatttaaa
     3901 atcctctgca caagggcgaa tgattcaagt tagtatccca gctaatgtac ccttacgcga
     3961 gtttgcttat gatgcagagg tagagttggt caatccaatc gtggataccg ttgccaatta
     4021 tgtttttcga gaaggaacta ccgttaattg gttcattaag gcggatgatt tagtcttgaa
     4081 gcggcagccg aatcaaggaa atcctacgaa ccaaaatgaa agaaagaaat aggtgaagat
     4141 tatggaaatt aatgttgaac atattttaga ttgtctcgat caatatggaa aaggcgagct
     4201 gactgaggaa caactcgtta aagcattaac ctctgatgaa aaaatgtttc tgattataca
     4261 tcaaggttta cttggagaaa atagcgacag tgaagaagac tctgatgtac tagactggtt
     4321 aggagaagaa gagtctttct ttatagttgt cgaagtaaat gaaaacttat gtcggcaggc
     4381 agaatccatt ttggaagaaa ttggtgtgga gatgccggat gctattgaag gctttttaaa
     4441 tcaattggtt gaaacgaaac aactcccctc ctcgtagcag taaatgacta gcataacttt
     4501 ttgaaattgc ctaatgaaat cctattgggc tttttcgatt ggaggtggaa accatcaaaa
     4561 tgtacaaagg acatcgcata cgcgcaggag atcagcactt ggtttatcac tttgttcttg
     4621 gctggctact cgctctgttc atcggttgga tgagcgtctt ttattttcaa gagtttagac
     4681 aatttgatat ttctaagcta tcactttcta ctattgaaat cgtttggtct ataaaagatt
     4741 tagtatgtct gcttggaagt ctcgcttttt caggagctat gatattgctt tatattcact
     4801 tttttcttga tcattggcga agcttgtggc atcgacaaaa gctagctcgg atgattctag
     4861 agaaccattg gtatgaagtg aaacaaactc aaagtgaagg ctttttcaaa gatttgaata
     4921 gtagccgaac cagagagact atcagttact tccccaaaat ctgttatcga atgaaagatg
     4981 gattactgtc tattcgcgtt caaatttcac ttgggaaata ccaagatcag cttttaaaat
     5041 tggaaaagaa gttagaaagc ggactgtatt gtgagttagt agaaaaggaa ctcaaagact
     5101 cctatgtgga gtatacttta ctgtatgata tgatcgccaa tcgaattggg atagacgaag
     5161 tagtggcaga aaatgggact ctgcgattga tgaaaaatca agtatgggct tatgattctt
     5221 taccccatat gttaatcgct ggtggcacgg gtggagggaa gacctatttc ctcctcacca
     5281 ttatcgaagc actattgaag tcagatgcag aattgtttat cctcgatccc aaaaatgcag
     5341 atttagctga tttaggtacg gtgatgcctc atgtttattc acaaaaggaa gaaatttctg
     5401 cttgtgtgga agatttctat gaacgtatga tggctcgtag caaggcaatg aaagaaatgt
     5461 ctaactacaa aacaggagag aattatgcct atcttggact tccacccaac tttttaatct
     5521 ttgatgaata cgtggcttat atggagatgt taacgacgaa agaaagtgcg gtgattttga
     5581 ataagctcaa acaaatcgtg atgttgggtc gtcaatctgg tttctttctg attttggctt
     5641 gccaaaggcc ggatgcgaaa tatttaggag acggaatccg cgatcaattc aactttcgcg
     5701 tggctttggg tcgtatgagt gaactcggat attccatgat gtttggggag gtcgaaaaaa
     5761 acttctttat gaagcacatc aaaggtcgag gttatgtgga tacaggtggc agtgtgatta
     5821 gtgagtttta tacgccactt gtaccaaaag ggtatgattt cttagattca attagtaaat
     5881 tagaaaagat gaatgattga gtgatcttca atactgactt tatacttcaa gtaacgtata
     5941 atacttttat atagtaggaa gaaaggggat aattaatatg gaaagtaatg attcttattt
     6001 agagtatgca aaagcaaata aggataagtt tatttcttat attatcaaag ggaaatcccc
     6061 cgaagttgga cgagacgcag tttttatggc agggagtcct ggggctggaa aatcagaggt
     6121 tgccattgga ttagctgaga attatgataa ccatgtgatt attgatgcgg attactttcg
     6181 cacccagttt cctgattata atggaaaaaa ctctagtctc tttcaaaaag catcttcttg
     6241 gttggtagag caggcattta gatatgtctt agatcatggc tattctttta ttctggatgc
     6301 aacttttgca atattaagtg ctgaaaaaaa tatctcgcgg gcgttgaaaa aacattttcg
     6361 agtgacgatt ttctatgttt atcaagaccc taaaatcgct tggaaattta cacaagaacg
     6421 tgaaattgca gagggacgtc atgtgccgaa aaaggcattt attaacgctt ttttcaaatc
     6481 aagagagaac attgcaaaag taaagagtaa acatccagat gtattgcttc atatcataat
     6541 aaaagattat acgaataata tttccgaagt tcattatgct acggataatc tagaattatt
     6601 tttaccacta gggtatagta ggaaagattt ggaggttcaa ttacatgact gagaaagaac
     6661 aagtaacgaa aattgtaaag aaatacaata agagcattgc ggatttatcg gaaaatgcta
     6721 ctgcaaaaga gttcaaaacg gtgattaaat atgtagccga tcaggccaat gaaaaacaac
     6781 ggaaattagt tggattaaat aaaaaatagt aggacaaatt aataatgtat gagccaagag
     6841 caaaatgttc ttggcttttt tctttgcaat gaatgaggta atgtatgaga tttttagatt
     6901 tattcgccgg tattggtggc tttcgtttag gtatggaaca ggcgagccat cattgtatcg
     6961 gtttttgtga aattgataaa tttgctagac gtagttataa agcaattcat gatacaagta
     7021 aggaggtgga aatgcatgac atcacaagtg tatcagatga gtttattcaa tccctcggac
     7081 cagtggacat cctttgcggc ggatttccgt gccaagcttt ttcaattgcg ggaaagcggc
     7141 aaagattttc cgatactcga ggtactttat tctttgaaat cgctcggttc accactcttc
     7201 tccgacctaa gcttttattc cttgagaacg tccgaggatt gctcaatcac gaaggagggg
     7261 ctacgttcga gacgatcctc cgaacgctgg atggattggg gtatgacgtg gagtggtagg
     7321 tgcttaactc aaaggcctac attccccaaa gccgggagcg gatattcctt atcggacatt
     7381 ctcgagacgc atgtaccgaa caagtatttc ctatcattgg accgtcttca acatctgatc
     7441 aaaacatcag aaatttggta aatatcaatc cttccaatcg aggaatgggt ggacaagtct
     7501 atggttcaga tggcgtagcg ccaaccttaa caggtgatga aggaatcaaa atcgcactgc
     7561 cagcaaggga tggaatctct gtagcaggac tattaccggg aaagtttgaa caaggtaatc
     7621 gagtttatga agttactgga accgccccca ccttgtcaac gaaacaaggt ggtacaaaaa
     7681 tcatgattcg aaaaaatgaa acagaatatc aagaggtgaa gccagaagac agtgtgaatc
     7741 tggcttttcc taattccaca agtcgccgag gtcgtctggg aaaacagagt gttcatacct
     7801 tactaacagg agaccaacaa gcgatcgtca cggatcagta tcaaattcgg aagctaacac
     7861 ctagagagtg ttggcgactt cagggctttc ccgattgggc ttttgatcga gattctcaag
     7921 tcaattcaga tagtcagttg tataagcaag ccggaaactc tgtaacagtt ccggtcattt
     7981 ttgatattgc cagaagatta aaagagttgg atgtacatgg tttatgatga gttgattgga
     8041 gaaatttatt gggtgattgg aaagattcag tcagacccag aattagaaga acaattgcga
     8101 cggttaaaat ttgatattcg caagaatggt gtcaaagtcg ctggcgatcc atatttaatg
     8161 aatgaggaga cggatgctcg aattgaaata aatcaagtaa ttgcagaatt tgagcggatt
     8221 gcggaccaag caaaagaacc agatattcgt caatacttgt tagaaatgaa agcagagttg
     8281 gaagtaaatg gtattacaga tgagtaacct aaaccaacac aactgactgt taggcgctga
     8341 ccgcggagga ttcgcgaagc agaaatcctc cgtggtgctg tgcctgttca tggacggcgt
     8401 tagccgacca tgatgtctca cccccgtatc taacaggggg gtacaaaaac acaaaaaata
     8461 agtaagcaac cattaagaat gcttgttgta tcaaagatag taagtaattt gtataggtgt
     8521 taactcattt gaaaaagtta acaccttgaa tttggaggtg agaacttggc acaacggaat
     8581 ttagattatc ggctattaaa agaccgacgc aacgaatacg gcgtttcaca aaataaattg
     8641 gctacggctt gtggacttag tcggccgtac ttaaatcaga tagaaaatgg tggcgtaacc
     8701 gcatctataa aaaccatgag gaaaattttt gaccaactgg aaagctttaa tcccgatttg
     8761 cctttgacgt tactgtttga ctatgtaagg attcgctttc ccacaacgga tgcacgaaaa
     8821 attattcaag aaattctcca tttgaaattt gagtatatgc tccatgaaga ttatgccttt
     8881 tactcctatc aagaacagta tgtcatgggt gatattgtgg tgatgttgtc tcatgaagaa
     8941 gataaaggtg tgcttttaga attaaagggg cgaggttgcc gacaatttga aactttttta
     9001 ctcgctcaaa agcgtagctg gtacgacttt ttcgaggatt gtctaaaggc cggtggcgtg
     9061 atgaaacgct tggacttggc aatcaatgat cgagtggggc tattggatat acctgagcta
     9121 acgaaaaaat gtcagaagga agaatgtatc tccttgtttc gtacctttaa aagttatcgt
     9181 tccggggaac ttttaaaggc tgatgaaaag gacggcatgg gcaatacctt atatatcggc
     9241 agtctcaaga gtgaggttta tttctgtttg tatgaaaaag actatgaaca atatatcaag
     9301 ttagggattc cattagacca aactgagacg aaaaatcgtt ttgagattcg actgaaaaat
     9361 gaccgtgctt ttcatgcgat tcaagatttg ttaaaaggtc gtagcattga gagcaccacg
     9421 ttttccatta tcaatcgtta cttgcgattt gcggataaag tagaaggtaa aaggcgaacc
     9481 aactggccct tgaatgaaca atgggggcgt tttattggac ggaatcggaa agagattcaa
     9541 ttaacttccg agccaaaacc gtataccatt gaacgaacct tgaattggct aggccgacaa
     9601 gtcgccccta cgtggaaaat ggctaaggag ttggatcgat tgaatcggac aacctatatc
     9661 caggatatgg tacagaatgc acgcttatca gaccgacata aaaagatttt ggagcaacaa
     9721 agtatggcaa ttgaaaattt gattatatga aaagaggaat cgtcatggga attgtaaaag
     9781 atattttttt ggtatttggt ggcagtatgc ttggtgttac tgtgatgtgc ttgatgaatg
     9841 caagtgcagc agcggatcga gcaatggaaa agaaaggaaa gtgacaagat gaatttcgga
     9901 caaaattt
//
LOCUS       DARDHA010000100         9725 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000100 DARDHA010000000
VERSION     DARDHA010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.101"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..195)
                     /locus_tag="SDA10_001952"
     CDS             complement(<1..195)
                     /locus_tag="SDA10_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240845.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
     gene            complement(211..825)
                     /locus_tag="SDA10_001953"
     CDS             complement(211..825)
                     /locus_tag="SDA10_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEG4240846.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(972..1655)
                     /locus_tag="SDA10_001954"
     CDS             complement(972..1655)
                     /locus_tag="SDA10_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEG4240847.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1764..2222)
                     /locus_tag="SDA10_001955"
     CDS             complement(1764..2222)
                     /locus_tag="SDA10_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEG4240848.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2306..4069)
                     /locus_tag="SDA10_001956"
     CDS             complement(2306..4069)
                     /locus_tag="SDA10_001956"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEG4240849.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4290..5750)
                     /locus_tag="SDA10_001957"
     CDS             complement(4290..5750)
                     /locus_tag="SDA10_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240850.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="SDA10_001958"
     CDS             complement(5958..6884)
                     /gene="glsA"
                     /locus_tag="SDA10_001958"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEG4240851.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6907..8334)
                     /locus_tag="SDA10_001959"
     CDS             complement(6907..8334)
                     /locus_tag="SDA10_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4240852.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8365..9579)
                     /locus_tag="SDA10_001960"
     CDS             complement(8365..9579)
                     /locus_tag="SDA10_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEG4240853.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2876 a         2032 c         1618 g         3199 t
ORIGIN      
        1 gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac catctaaagt
       61 aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact gcccttgcag
      121 aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg atggtttata
      181 gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg tgaaaatttc
      241 tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt ttcttggatc
      301 gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct gtacttctag
      361 caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt catataagga
      421 tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta ctgaatcagt
      481 taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg ctgctgtttt
      541 atttacttct tctacgttgt cctcatcaag gatatggata acagcttctc ttgtctcaag
      601 aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca aggataagat
      661 gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca ctatatcttt
      721 tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa tcagcaactt
      781 gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt cgccttcttt
      841 ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg cttttttcat
      901 cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag aatctgtgcc
      961 gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt agtgatcaat
     1021 tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc atatgctttc
     1081 tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc gacttgttct
     1141 atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata ttctgtatag
     1201 cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag cgcttcctca
     1261 aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg gacaagttca
     1321 gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat gatttctgct
     1381 tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac ttctgaccca
     1441 tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt gatttcattg
     1501 atatcttctg tcgtatttgc tcgaatacca actgctccgc cttcttttgc agccaaagcc
     1561 attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg acaagaaacg
     1621 acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt aatgttatat
     1681 aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagacaa taatgagcta
     1741 tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc gtatacgacc
     1801 agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt aaaaaactca
     1861 gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt ccaagcatgc
     1921 tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat gatacaaact
     1981 ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa aggctcattc
     2041 tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt tacaactagt
     2101 tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc taatttttct
     2161 gattgataac taggatattt ttcttggaac tttttgacat attcttctac ttgctgatac
     2221 ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta gtataaacaa
     2281 ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga ctatcttcaa
     2341 cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga acgctatccg
     2401 tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca taataacgac
     2461 tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg actaattcgt
     2521 gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt cctggattga
     2581 tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt cctaattcaa
     2641 gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt tgataggctg
     2701 gaatactata atcatttgcg atagctgtac catgttctac accatgtgta ggaattcgga
     2761 ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc atatcctctt
     2821 ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga atgtcaaatg
     2881 ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa ataatcactg
     2941 gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc aagaaagtac
     3001 ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg aaagtgatag
     3061 agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct tggaatgttt
     3121 tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct gtacttgttt
     3181 gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca gaagttactt
     3241 gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc attttattat
     3301 cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca atcggatgat
     3361 caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt ccattatcca
     3421 cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta ccgttggttt
     3481 gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata gacatctcat
     3541 tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc aatccttcaa
     3601 aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt cctgattctt
     3661 tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta gatgtgtgcc
     3721 ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc acttcacggt
     3781 aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca taagactgat
     3841 gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga aacgcaatcg
     3901 ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg tccttttgca
     3961 agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat aattttttga
     4021 catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata atacattctc
     4081 tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct attctactac
     4141 ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc ttgttatata
     4201 agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa ttaaaaagct
     4261 ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa taggtgaagt
     4321 tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg atagatcttt
     4381 gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac cactcttttt
     4441 ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat aggatttatt
     4501 tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa ttgtttgact
     4561 gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc actgttgatc
     4621 aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag ttggatttga
     4681 tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag ttgttctttt
     4741 tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca caacgtacgg
     4801 acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat ttcagcgata
     4861 ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa atttttataa
     4921 acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt tctactgttg
     4981 aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc tttttgctct
     5041 tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt cttatcttct
     5101 tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc tcgtatgata
     5161 aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac ataatcaatc
     5221 aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc ccaattgaca
     5281 taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat gatttcttta
     5341 tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat tgtatatact
     5401 ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat accattaaaa
     5461 taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt gagctcagaa
     5521 agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac ttgttctttt
     5581 atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt tggtgtagtc
     5641 acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa taattgttca
     5701 cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat tcctgataca
     5761 cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat ttttgtttcc
     5821 tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa
     5881 acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca
     5941 gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc
     6001 aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct
     6061 atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt
     6121 acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt
     6181 gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt
     6241 cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag
     6301 cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct
     6361 ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt
     6421 tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc
     6481 tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca
     6541 ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg
     6601 aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct
     6661 ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg
     6721 gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt
     6781 ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag
     6841 tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt
     6901 cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat
     6961 aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc
     7021 tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc
     7081 taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag
     7141 actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag
     7201 gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct
     7261 taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc
     7321 aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc
     7381 tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa
     7441 acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa
     7501 taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt
     7561 agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa
     7621 cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt
     7681 ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga
     7741 aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac
     7801 ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc
     7861 gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca
     7921 gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc
     7981 cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat
     8041 ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac
     8101 ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa
     8161 tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta agctgaagcc
     8221 tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg tcatagccaa
     8281 aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt gcatgaatct
     8341 tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa
     8401 tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa
     8461 aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa tcacttgaat
     8521 ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg caaagatccc
     8581 tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat caagtgcatc
     8641 atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac ccgcaatgat
     8701 cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa tggctgccaa
     8761 tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc
     8821 aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt
     8881 gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc agccaaacca
     8941 aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc tttctcctaa
     9001 aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt
     9061 gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa atccgccacc
     9121 ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa caagtataat
     9181 atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac
     9241 gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac catgaaacat
     9301 gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc caataacacc
     9361 gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa tcgttgttat
     9421 cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc gtgctaatcc
     9481 gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac ataaaatcat
     9541 gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct cctttctacg
     9601 attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga attaatactt
     9661 gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt tttcgttttt
     9721 cacac
//
LOCUS       DARDHA010000101         9713 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000101 DARDHA010000000
VERSION     DARDHA010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.102"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            33..656
                     /locus_tag="SDA10_001961"
     CDS             33..656
                     /locus_tag="SDA10_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240854.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /locus_tag="SDA10_001962"
     CDS             1106..2155
                     /locus_tag="SDA10_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4240855.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="SDA10_001963"
     CDS             2152..3033
                     /locus_tag="SDA10_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4240856.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="SDA10_001964"
     CDS             3036..3806
                     /locus_tag="SDA10_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4240857.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="SDA10_001965"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="SDA10_001965"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEG4240858.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="SDA10_001966"
     CDS             5117..6280
                     /locus_tag="SDA10_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG4240859.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="SDA10_001967"
     CDS             6397..6807
                     /locus_tag="SDA10_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG4240860.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="SDA10_001968"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="SDA10_001968"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEG4240861.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="SDA10_001969"
     CDS             9380..9664
                     /locus_tag="SDA10_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240862.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
BASE COUNT         3371 a         1646 c         2087 g         2609 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgt
//
LOCUS       DARDHA010000102         9618 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000102 DARDHA010000000
VERSION     DARDHA010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9618
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.103"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            220..459
                     /locus_tag="SDA10_001970"
     CDS             220..459
                     /locus_tag="SDA10_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240863.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(821..1081)
                     /locus_tag="SDA10_001971"
     CDS             complement(821..1081)
                     /locus_tag="SDA10_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240864.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1266..1763)
                     /locus_tag="SDA10_001972"
     CDS             complement(1266..1763)
                     /locus_tag="SDA10_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEG4240865.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1893..2603)
                     /gene="budA"
                     /locus_tag="SDA10_001973"
     CDS             complement(1893..2603)
                     /gene="budA"
                     /locus_tag="SDA10_001973"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEG4240866.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2616..4280)
                     /gene="alsS"
                     /locus_tag="SDA10_001974"
     CDS             complement(2616..4280)
                     /gene="alsS"
                     /locus_tag="SDA10_001974"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEG4240867.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4488..5150)
                     /locus_tag="SDA10_001975"
     CDS             complement(4488..5150)
                     /locus_tag="SDA10_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG4240868.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5160..5975)
                     /locus_tag="SDA10_001976"
     CDS             complement(5160..5975)
                     /locus_tag="SDA10_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4240869.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6236..6679)
                     /locus_tag="SDA10_001977"
     CDS             complement(6236..6679)
                     /locus_tag="SDA10_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEG4240870.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6710..6823)
                     /locus_tag="SDA10_001978"
     CDS             complement(6710..6823)
                     /locus_tag="SDA10_001978"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG4240871.1"
                     /translation="MEADSSNTLALLLEKLIRDYPSVQFHIHSGTSDDITE"
     gene            complement(6813..7151)
                     /locus_tag="SDA10_001979"
                     /pseudo
     CDS             complement(6813..7151)
                     /locus_tag="SDA10_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(7139..7516)
                     /locus_tag="SDA10_001980"
     CDS             complement(7139..7516)
                     /locus_tag="SDA10_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG4240872.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7740..8933
                     /locus_tag="SDA10_001981"
     CDS             7740..8933
                     /locus_tag="SDA10_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4240873.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9099..9521)
                     /locus_tag="SDA10_001982"
     CDS             complement(9099..9521)
                     /locus_tag="SDA10_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4240874.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2868 a         1909 c         1591 g         3250 t
ORIGIN      
        1 ttttcactta taaatttttt cataaaaaaa gcgtttagag gcataatcgt taatacagat
       61 ggtaaccaat gtatctggtt tgaactggga tatcaccctc tccatatatg tattactaag
      121 actaatagat actataaaag acttgatagt tttgcattga ttttgcctaa tactgagcat
      181 aggtgtatgc taaacgatac tataaggagt gtgtacataa tgaaaagaat tctgtttatc
      241 agtttatcaa atttattgtt acttactgct tgtgggactt ctgcatcaaa gaacatacct
      301 tcagatacgc aaatttcgaa tagtatcgat tcaacaattt ctacaaatga gaaaaattct
      361 ccggtagaaa cttccgatat aaaagtttct acctctgcaa aaaacagggt aaagaaactg
      421 tcagcacgtc tcctttattt caaacaaact ctgaattagc ttcttcatct tcttcagcaa
      481 ataatgatga tagtttcgcg tttactccta actaaatgca acctttacaa atgctttacg
      541 ataacaagcc tgagctaaat gatcctgata tcagttttgc attttttgaa atgattggat
      601 cggatgatct ttttaaaact actagtttgt ctatccgaaa ataggacagt tctggcagta
      661 taggttttta tcgagtttct ccaccaggcc tcgttacaga gacagattct ctaggcgaac
      721 cttttgaata aatcaaaaat catgaacaat cactgccaac taaataaagt gccttcaaaa
      781 gcttggtctt ctcgcttttg aaggcacttt atttaaatct ttatgaaact aaagtatgtt
      841 catatttgtt gctatagtta tatagatatt ctgttgtttc caactcataa acgtgagtat
      901 attttgccac tacttttccg catttttcta atatttcatt gcgatcgacg tcatgatatt
      961 cgtctacgca gatcacaaca caattttcta atttactgat tacttctcca accacttttt
     1021 gtttcaagcc aataggttgg caaatatatt tttttcctaa aatgatagtt tcgttattca
     1081 tatttcatcc atccttaatg ttttttcatt attcaaaatg ataataccat taaaatatga
     1141 ttgtgaaaat caataacact tgtgatttta taaaaaaata aaaaaaccga aaaacagaaa
     1201 tgaccactgt ctttccggct ctttttattc ttgatttctc ttcttttttg tttcaataga
     1261 cgattttagt tattaataaa tgaaaaagca tttatattaa aatttgctaa taaaagatct
     1321 acatattttt ctagaaatgc ttgatcgatt tcatcatacc ctttcgttac tccgcaatcg
     1381 atatctaaaa caccgattaa attaccttct tttaccattg gcactacgat ttctgattgt
     1441 gcggctgaat cgcaggaaat gtaatttgca tgggttttga catcatcaac gatcaatgtt
     1501 tcttgttttt cagctgattc tccacaaact ccttttccca tcttgatacg tgtgcaagaa
     1561 actcgccctt ggaacggtcc taaaatcaat tcattaccat cgaataaata atatcctgca
     1621 aagacagtct caggcaaagt ctcattcaac aatgcagatg aatttgctaa atttgctaac
     1681 cagttatctt ctgcttctaa taatccttgt tgctgtaata ataaaatttc gtatgcatct
     1741 tttttctgtt tttctgtcca catgataaaa accgctcctt tttgatctat ggagaagatt
     1801 atagctcagt ttttttgtaa acgctaggtt tcctcctcaa aaaaacgaga tacgtcaggt
     1861 taatcacctt gaacgcatct cgctgtcttt ttctattctg cttgttggat ttctgctgct
     1921 aaatcagcat aatcaatcgt ttcgtgaaca aaagtcggat catcaactgg aaaatgctgt
     1981 acaagggacg agatgcggct aatctcaact tttccttcag ccacttcaaa atccattaca
     2041 tgtccgccaa atgtatgtgt atcatcgata aaatgcaggt gaaatcccgc tgcagcaact
     2101 ccttggaata attcaggcgt aaagaacccg actaatgttc cttggatatt gctttgttgg
     2161 aattctggtt gagactctga aatttttgct agtcttctgt aaggtttttc ttgcttcggc
     2221 atgatacgga tgtgcatatt tttgaataat ccagttatct tcactgccaa aaataggttt
     2281 tccccggctt ctcttactaa tatgtctttt ttcaattgtt ctccatctgc agtttttgag
     2341 acagaaaaac tagtatctgg cgtaaaatct gtaattgctg catagggggt aagttcactt
     2401 ccatctaatt gggcaactga gccatctgat cttccttgat aagctacgcc atctaaaaag
     2461 atgacttcac catcaagccc atgcaatgtt cccaatccca gagttccttt ttctaatata
     2521 tgagcaatcg tttctgttcc atccatcaag ccggccatca aagctcctaa tgtcccgtgc
     2581 tggtataaga ttttttcagt catgtcgaca cttccttaat agaattgatc tggtaagatt
     2641 gtttctccca atttctcatt atcacggtaa tcaatcggaa tatcaataat tacgggtcct
     2701 tctgtcgcaa atccttcttc taaagctttt tctaattcct ctacacttgt agctcggatt
     2761 cctttagcgc caaaagcttc tgcatatttc acaaaatcaa ctggaccaaa gtctacacct
     2821 gacgcccgct gatatttcat tttttcttgg aactctacca tattgtagtg tccatcattc
     2881 caaatcaaat gaacgatatt caatttttta cgtaccgccg tttctaaatc ctgggcagag
     2941 aataaaaatc ccccatcgcc ggaaactgat acgatttgtg tatttggacg caccaatgca
     3001 gcagaaatcg cccatggcaa tgcaacacca agcgtttgca taccattact gaataacaaa
     3061 tgacgaggtt cataacttct aaagtggcgt gccatccaaa tataatgact acctacatct
     3121 acggtaacag tcatatcatc tgttactttt gactgaagcg tgttaataac ttctaaagga
     3181 tgcaagatac ctgcttctcc ttttgattga acaatgtccc gctccgttaa ttttgcttga
     3241 agagatgcta aatattcttt tgcatcttct gacacttgtt gaggctctaa tgaacctgtc
     3301 aataaatcaa gagtggctga gatatcaccg atcaattctc tttcaggctg cataaaagga
     3361 tcgatttcag ctggcgcttc atcgatcaca atgatccgcg catctttttc cgcattccag
     3421 tttcttgctt catattcgat tggatcatag ccaattgcaa taactagatc acttcgtttc
     3481 aaaagcatat ctcctggttg gtttctaaat aaacccacac gaccaaagaa atgcgcttct
     3541 aattcacgtg agatcactcc agctgcttgg aatgtctcta caacaggcaa tgctgtcttt
     3601 tcaactaatt gcctgatggc taacgtttct ttttcacttg atcctcgcat acctactaat
     3661 agtaccggta gttttgcttc tctaatgcgt tcagctaggt aacggatatc atctgcagaa
     3721 gcagaaccta atttaggatc gctcaatggc ttgatgacat taccttggac aggtgcatcc
     3781 actacatctt gcgggatact gataaagctc gcgccttttt ttgaactctt agccatacga
     3841 taagcattgg caatcacttc tgaaattgtt tctggatctt ggatttctgc actgtatttt
     3901 gtgattggct ggaacagtgc tgcattatcc atgctttgat gggtcaactt caatagatcg
     3961 cttcgtttta cttgtccggc aattgctaaa actgggtcac cttctgctgt cgctgttacc
     4021 aaacctgtag ctaagttact agcaccagga ccacttgttg cgataactac tcctggctct
     4081 cctgtaatac gtccgattgc ttgtgccata aatgcagcat tttgttcgtg acgagtcacg
     4141 attaattcag gaccttgatc ctctaattca ttaaatacac catcgatttt tgcacctggt
     4201 ataccgaata cataatcaac atgatgatta atcaaacttt ctactacaga tttcgaacct
     4261 tgtttcattt tttcagccat tgaacatcgt cctttttatg ctttattttt aattattagt
     4321 tagaaataat cacaaataag tgatacagtt tctaaacctg tttcatacaa aagtatagca
     4381 cgttttattt catttgttta gccatatgtt tcaaaaaatg aaaaagaatg acagttttta
     4441 cataaaaaaa actgtcattc ttaattgaaa gtttcaattt ataaagtcta cagatccata
     4501 gttctcatca atttacgatt gttgaagtaa atcaaaccaa atacgataga aggacctagt
     4561 agaatacacc attggggctt tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa
     4621 tctagtaaac tcacatacca ttttttcaac gtatagtaga acgctatccg tagaacttga
     4681 tttggtttcg acgctggatt tctcacctga aaataccagc agttggtgct aaaggaaact
     4741 gataaacaag caagtaaaac aaacaacgga atcaaccatt ttccataaga aaaacgtata
     4801 aaaaagaaac aatcaaaaat cattatcaca ctaactatcc aagcaaatag ccaataaaaa
     4861 aatcccttta gccaaaattg gcgaaacatc tttatgaaca aacgtactgg tgaaaaacta
     4921 ttttcttttg ctaattcttc tgtcatacct atcatcacga tgcaacctgg accaataaaa
     4981 agtaaagaaa aaataaagga aaagacattc cttccatcta tcgacaaaag gaacgcagct
     5041 gctatataca agatattgac acaccaaaaa caaaggctac ttgccaatga ttgatacaac
     5101 caataaaaga attccatata aacatttttg tcaaaacgct gctctttcat aatcacctcc
     5161 tattccttca ttcctgatga agcgatccct tctacaaaat atttttgcat cgctaaaaat
     5221 ataaaagcaa caggaagaac agaaacaact gaggcagcca tgattaatgc gtactctgca
     5281 tcaaatagcc caataaacat tttcaaccct aactggattg ttttattttc cgtagaagac
     5341 aaataaatga atggccccat ataatcgttc catgtattaa cgaatgtgat gatcgtcaaa
     5401 ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc gatagattcc ccactctgat
     5461 aagccatcga tccgcgcact ctcacataaa gaatctggga tactactgta atattgcttc
     5521 aacaagaaca caccaaaagc attgaacgct tgtaatagca ccaacgacca caacgtatct
     5581 gttaaaccca cttttctcat cataatgaac tgcgggatca tataagactg ccaagggaca
     5641 gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc ctttaaaatt tagtttagaa
     5701 aacccgtaag cagcaaaact agaagtaaac aactgaatca acgtgatcac aacgcttaaa
     5761 aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag accaaataac cagataattc
     5821 tctaaatgcc aagttttagg gatccattga atcggaatcg tgaacacttc attatttgct
     5881 ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg tcaataaagc taatacaatc
     5941 agcagaatca tacccaacca ttgaataaag ggcatgtttt ttgaacgaat ttttgctttt
     6001 tttctttcat ttcgaacacc tccaaagtta taatcttcat ttctttgtaa tgattacaat
     6061 ccagaacaac ggttattata gctgattcat ttccgttctt cttatttttg taagcgctta
     6121 tttaacaagt tatgtaaatc tctttatttt tttgatttaa aaatgaaaaa atacaaaagc
     6181 aagactgtca tacgtctcgc ttttgtattg aaatttcttc gattagagat acttattact
     6241 gttcaaaagc ttctttgaaa cggtgaatca attcattgac tacaggtgat aaaatccgat
     6301 tctttttcca gactagcatg cagttcgttt caatcattgg gtcaagcgga acgaatgtca
     6361 ctcgctttga atgatgactt acagcacctt ctatgactaa agcagcacct acctgattct
     6421 caattagcgc aaatacattg aaaatcaaat tgtacgttcc tgtgatcagc aactgctcct
     6481 tttttatgcc taaccagtca gcaaacattc gctgtatttc tggacgcttt gagatcaaaa
     6541 gaggtacatt cgcaaagtct tcttttgtta tcacgtcttt ttttgctaaa acagaatttt
     6601 ctaaaatgag caatccccat ttttcctttc ttggtaacaa aatcgtttcg tatttctctg
     6661 tatctactgg atctagcaaa atcgctaaat ctaacaggct tttttctagt cactccgtga
     6721 tatcatcact tgttccgctg tggatatgaa attgaacgct gggataatca cggatcaact
     6781 tttctagtaa tagtgccaat gtattcgaac tatcagcttc cacacagccg atcgtaaatc
     6841 ggccgctgaa taattgtttt tttctgttct cgaattcttg agtggtttga ttcgttaaat
     6901 ctaaaatttc ttctgctcgg cttttttaga aaataacctg cttctgtcaa gatcattttg
     6961 cggttttcgc gtacaaacag ttctgttcct agttactctt ctaactcctt cagttgtcgg
     7021 cttaaggttg gttgtgtaag atgtagaact tctgcggctt gagagattgt tccttcttct
     7081 gcaattgtcc aaaaatattt tagtaagcgt aactccatag cgatgctcct tgattttttt
     7141 acttgataca ttccatttta tttctagata acacacgatc atatttcttg atttttcctt
     7201 gtaaacgttc caaacattct tgcatttcag caattttttt cgtcaaaagt tcactttctt
     7261 caatcaaaag cttacgccgc tcaaatacag ttgcttctcc ttcgctagat aggcgaatat
     7321 attcaatcaa agactcgatc gataatccgg catttcgcat acagatcgta aagtcgaccc
     7381 attttaggtc ttcttctgta taatcacgga ttccgccttt tgtccgatta actggtggaa
     7441 tcaacccgat tcgttcataa tagcgcaatg tatcggccga aatgcccttt tcttcactga
     7501 cttgtttgat attcatctga tcactctttc tcttaatttc ttgccacttt acttatgtat
     7561 tttatttatt ctacaacttt aagtgtactc caagtcaata ctcatttcta ttaaaactaa
     7621 gctggattat ctatatcggt tgtttgtgta ttatttcaaa taaaaaagtc ttgttttttt
     7681 attctttttt ggatatactt ttaacaattt agggaaattt taggaaaagg ggtataaaaa
     7741 tgacaacgta tcagcgaaag gtattagtat cgacatcggc gggaattgct ttagagaata
     7801 tggatattat gtttttagct ttttcattat cttcaatgat tacttctttt catattagtg
     7861 gtacgcaagc gggtttcatt tcaacgatca ctaatttggg gatgctcatt ggaggtattt
     7921 tttttggcat catagctgat aaatatggac gtgtgaaagt attttctcaa acggtccttc
     7981 tgttttcgat tgcttcttta ttgatgtatt ttgcttcaaa catctacttg gtttatctct
     8041 ttcgctttat cgcaggtgta ggagcgggag gagaatatgg ggcgtgtatg tccttgattt
     8101 ccgaaagttt ttcaaaaaaa caaattggac gagcttcttc ggttgctggt attggtgcgc
     8161 aggtcggtgc ggctttggct gcgattttag ctgctgtgat cattccaacg tttggttgga
     8221 agatgttgta tgtcgtaggt gtactgccgg tgttgatggt cttgttcatt cgtcgcggct
     8281 tgactgaacc aaaggaattc aaatctgtac aatctgccga aaagaaaacc aaacttagca
     8341 gactgtttga atcaaaacat cttgcttggc aaacatgtgg ccttagtatc atggtaactg
     8401 tccaaattgc cggctatttt gggttaatga attggctacc ttcaattatg caaaatcgat
     8461 tagggctttc ggtttctggg tcttcattgt ggatggtcag tactatttta ggcatgtctt
     8521 tggggatgat gacttttggc ataatcatgg ataaattggg gcctaaacta gccttttctt
     8581 tgtttttaat ttgttcatcc ttatctgtct atctacttgt gttagcccat agccagtgga
     8641 gtttggtagc agccgctgtg atagtcggct actttatcaa tggcatgtat ggtggatatg
     8701 gcgcagtgat tagtagtttg tatccaacag aaatccgcgc gacggcgaat aatttcgtta
     8761 tgaaccttgg acgagctgtt ggtggttttt cttctatcgt catcggtttc ttgatggatc
     8821 attatagttt aactgccgta atcttgtttt tgagcggtat ttacctagtc agtctgctgg
     8881 tcttgacaac tcttactggt gttacagcat taagggaaag cttgcagcat tgatagttga
     8941 gtctgtaaat gacaagaaag aggtgtggaa aaagcttgca tctgcggtaa taagaccagc
     9001 caaatcaaaa acatgagaca ctgtttccgt ttggctggtc gtattacttg atgcagggca
     9061 gctttttcaa ctgctaattt ccttctatct ggcggcgact attctttgac gtccgtcatt
     9121 tcaaagtact ttactaataa cttagcataa cgaaaaactg tggatacact cgtatattga
     9181 gcgtctgcat aatcttccaa atttttctca ggatgttgat acatgaattc aatcaaatga
     9241 taattatatg attttctgta gaaatgaaga taaataatgt agattgggaa gttctcctgc
     9301 ttttccatag aaaagcgccg attattctca ttcttcaaaa tgaccttatc aggatattgt
     9361 tggaacaatt cttctaaatc tgttagataa ttcaagatag acttacttga taccttcaat
     9421 tcgttttgta gttcttctat tgaacaccaa cgttctgttt caagcagtaa atgaatcatt
     9481 ttaattttct tttgagaaac ttttgttaaa aaagtctgca tattttctcc tcctgtatct
     9541 actttactat actacataca agaaaaaaac ggatacaaat atcatattgt cacaccgtca
     9601 atagtgcata aacctgtt
//
LOCUS       DARDHA010000103         9595 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000103 DARDHA010000000
VERSION     DARDHA010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.104"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            99..293
                     /locus_tag="SDA10_001983"
     CDS             99..293
                     /locus_tag="SDA10_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240875.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            562..861
                     /locus_tag="SDA10_001984"
     CDS             562..861
                     /locus_tag="SDA10_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240876.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(958..1392)
                     /locus_tag="SDA10_001985"
     CDS             complement(958..1392)
                     /locus_tag="SDA10_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4240877.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1624..2334)
                     /locus_tag="SDA10_001986"
     CDS             complement(1624..2334)
                     /locus_tag="SDA10_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEG4240878.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2324..3184)
                     /locus_tag="SDA10_001987"
     CDS             complement(2324..3184)
                     /locus_tag="SDA10_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEG4240879.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3189..3827)
                     /locus_tag="SDA10_001988"
     CDS             complement(3189..3827)
                     /locus_tag="SDA10_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEG4240880.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3842..4141)
                     /locus_tag="SDA10_001989"
     CDS             complement(3842..4141)
                     /locus_tag="SDA10_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4240881.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4184..5668)
                     /locus_tag="SDA10_001990"
     CDS             complement(4184..5668)
                     /locus_tag="SDA10_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEG4240882.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5688..6149)
                     /locus_tag="SDA10_001991"
     CDS             complement(5688..6149)
                     /locus_tag="SDA10_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4240883.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="SDA10_001992"
     CDS             complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="SDA10_001992"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEG4240884.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7452..8222
                     /locus_tag="SDA10_001993"
     CDS             7452..8222
                     /locus_tag="SDA10_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG4240885.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8495..9322)
                     /locus_tag="SDA10_001994"
     CDS             complement(8495..9322)
                     /locus_tag="SDA10_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4240886.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2989 a         1954 c         1503 g         3149 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa
      781 aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag
      841 gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat
      901 cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta
      961 tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc
     1021 catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc
     1081 atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt
     1141 caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat
     1201 aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc
     1261 attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt
     1321 tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga
     1381 atcaatattc atttttttct tcctttctta tacaattctc cctctaataa tacagttctt
     1441 tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt
     1501 cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt
     1561 cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca
     1621 tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa
     1681 atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca
     1741 aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa
     1801 cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt
     1861 aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt
     1921 cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata
     1981 agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt
     2041 aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga
     2101 tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc
     2161 tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg
     2221 gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag
     2281 atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc
     2341 cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt
     2401 cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca
     2461 aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact
     2521 ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat
     2581 cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta
     2641 ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg
     2701 aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag
     2761 aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt
     2821 tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg
     2881 ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag
     2941 aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc
     3001 tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc
     3061 gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg
     3121 aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag
     3181 ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc
     3241 ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca
     3301 aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca
     3361 atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca
     3421 tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt
     3481 tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc
     3541 tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca
     3601 acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta
     3661 tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt
     3721 gtgcccactt caataatatc tactacatca ccaactttca taacatctcc taatgcatgt
     3781 tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc
     3841 tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg
     3901 acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat
     3961 tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg
     4021 aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca
     4081 cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca
     4141 tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt
     4201 ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt
     4261 gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca taacctagga
     4321 ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac
     4381 caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga
     4441 ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa
     4501 ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg
     4561 atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc
     4621 caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa
     4681 cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga
     4741 atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca
     4801 taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt
     4861 tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca
     4921 tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt
     4981 gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa
     5041 atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt
     5101 cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc
     5161 ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa
     5221 aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg
     5281 tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca
     5341 tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc
     5401 caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga
     5461 ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg
     5521 ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta
     5581 ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg
     5641 tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct
     5701 gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca
     5761 tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt
     5821 gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa
     5881 cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt
     5941 tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct
     6001 acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca
     6061 agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc
     6121 aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg
     6181 gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact
     6241 caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt
     6301 taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat
     6361 cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg
     6421 actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt
     6481 caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat
     6541 tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt
     6601 ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt
     6661 cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga
     6721 ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt
     6781 gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa
     6841 ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt
     6901 ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca
     6961 tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat
     7021 gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt
     7081 tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt
     7141 ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact
     7201 tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt
     7261 ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg
     7321 tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag
     7381 atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg
     7441 aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg
     7501 aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat
     7561 ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca
     7621 attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg
     7681 aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc
     7741 tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt
     7801 tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt
     7861 atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat
     7921 taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg
     7981 tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca
     8041 acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa
     8101 agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg
     8161 aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt
     8221 aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg
     8281 ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa
     8341 tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat
     8401 caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg
     8461 gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct
     8521 ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta
     8581 aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca
     8641 atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg
     8701 ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga
     8761 ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt
     8821 tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta
     8881 agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg
     8941 catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta
     9001 aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt
     9061 ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt
     9121 cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta
     9181 agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac
     9241 aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta
     9301 atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta
     9361 tatcatacac aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa taaaaatttt
     9421 aattttaaaa taatattcaa attaaacaac tggcgtatct gctccttgat ttctttttgt
     9481 gttatctgaa taattatcgt ttacagttat aacacatagt gaaaattgtc atggcgagaa
     9541 agtctaagaa tatttttaaa cataactatt tctttcttac gtgtgtataa tgacg
//
LOCUS       DARDHA010000104         9573 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000104 DARDHA010000000
VERSION     DARDHA010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.105"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            191..1072
                     /locus_tag="SDA10_001995"
     CDS             191..1072
                     /locus_tag="SDA10_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4240887.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            1085..2695
                     /locus_tag="SDA10_001996"
     CDS             1085..2695
                     /locus_tag="SDA10_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HEG4240888.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2772..3431)
                     /locus_tag="SDA10_001997"
     CDS             complement(2772..3431)
                     /locus_tag="SDA10_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG4240889.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3714..4379)
                     /locus_tag="SDA10_001998"
     CDS             complement(3714..4379)
                     /locus_tag="SDA10_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEG4240890.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4526..5131)
                     /gene="dhaL"
                     /locus_tag="SDA10_001999"
     CDS             complement(4526..5131)
                     /gene="dhaL"
                     /locus_tag="SDA10_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEG4240891.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5159..6169)
                     /gene="dhaK"
                     /locus_tag="SDA10_002000"
     CDS             complement(5159..6169)
                     /gene="dhaK"
                     /locus_tag="SDA10_002000"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEG4240892.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(6144..6590)
                     /gene="dhaM"
                     /locus_tag="SDA10_002001"
     CDS             complement(6144..6590)
                     /gene="dhaM"
                     /locus_tag="SDA10_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEG4240893.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6693..7013
                     /locus_tag="SDA10_002002"
     CDS             6693..7013
                     /locus_tag="SDA10_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEG4240894.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(7125..7994)
                     /locus_tag="SDA10_002003"
     CDS             complement(7125..7994)
                     /locus_tag="SDA10_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG4240895.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(8090..9393)
                     /locus_tag="SDA10_002004"
                     /pseudo
     CDS             complement(8090..9393)
                     /locus_tag="SDA10_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(9390..9551)
                     /locus_tag="SDA10_002005"
     CDS             complement(9390..9551)
                     /locus_tag="SDA10_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240896.1"
                     /translation="MKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
BASE COUNT         2578 a         1994 c         1791 g         3210 t
ORIGIN      
        1 tattataact atctattgag aaagtatcaa gctagcaatt ccttgtttat gaattcattg
       61 acagaaattt ataaaaagaa tattatgtac taaaataata aaaaaagttt tataaaaagg
      121 tataaaacct ttttattaaa ctatttaatc ttgtttagta tatattttta gaaaaaggag
      181 tggggttttt atggcagaaa tcgtcaaagt cacacatttg aaaaagagct ttggtcgttt
      241 cgaagcttta aaagatgtct ctttcaccgt acattcaggt gaagtcgttg gatttatcgg
      301 accaaatggt tctggaaaat ccacgacgat tcggacactt ttagggatta taaaaaaaga
      361 cggcggaacg gcaacgattt ttgggcaaga tgtatgggaa gatagtttat ctatccatga
      421 acgtctttct tatgttccag gcgatatctc tttgtgggga aatttgactg gaggagcgat
      481 catcgatctc ttcatgaagc ttcatggaaa agggagtgct caaaaaaaag atgaactgat
      541 tcaacgtttc tctcttgatc ctaagaaaaa agcaaaaaac tattcaaaag gaaatcgcca
      601 aaaagtgggc ttgattgctg ctttggctgt tgattcggat ctctatttat ttgatgagcc
      661 tacttctggt ttagatcctt tgatggaaac gattttccaa gaagaagttg aaaaattgaa
      721 agaacagggc aaatccatcc tactttcttc tcatattctc agtgaagtcg agcgactagc
      781 ggataaagtc gtgatcatac gcgaaggaac agtcgtagaa gaaggaacac tcgatgaatt
      841 gcgtcatttg actcgttcga ccatcactat agaaacagaa gcagagaata gtcacttagc
      901 acaattgaac ggtgtccatg actttgtgca aaaaggtcac ctcgctcaat ttgctgctga
      961 taacgaagct ttcgatgtga ttttagctga agcgtccaaa ttaggtgtga aaaaatttga
     1021 atctattcca ccgactcttg aggatttatt catgagacat tatgaaggat gaggaggaag
     1081 cgaaatgact gataaatttt cacgctggtc cacccttttt tctcaaaacc ttagccgtga
     1141 ctggaaaaag atcctgatat ggctagtcgg tgtcactgct ttttctggtg gttttgtccc
     1201 tgcgtttgaa gaattgactt ccggacaagg attagctgga ttatacgaaa ccttgcaaaa
     1261 cccggcaatg attgcaatgg tcggaccaac tccagcttcg aatgctgctg agtatacttt
     1321 aggagcgatg tatgcaaatg aaatgttgct tttttgcagt ttgtttgctg ctgtgatctc
     1381 tgttttacac gtggtgagcc aaacgagaaa aaaagaagaa cttggtctga ttgaacttat
     1441 tcgttccttt cgagtcggac gtcaagcaaa ctctttagct gtcatcgctg aaagtattct
     1501 gatcaatttg cttttagcaa tatcgatcag ctgtacgatg atttttttcc aagcagattc
     1561 gatcactgca gaaggtgctt ggctttttgg gctatctgta ggtagtatgg ggattttggg
     1621 gagtgccgtt gcactgtttg ctgcacaact gatgccaagt tcagccagtt cgaatggtct
     1681 gtctttagcg tttttaggtg gactttacct tctacgagca ataacagatg tagctgattc
     1741 ctccctttcg atgtggaatc ctttaggctg gtcttatttg acctatccat ttacaactaa
     1801 caactggatt cctttgcttt atttgttttt gttcagtcta atcttgataa tcagttcatt
     1861 tgttttagag gaacatcgag atttgaacac cggctatatc cccgaacgtg aaggaaaacc
     1921 gtatgctaaa cgttctcttt tatccttgcg tggtttatta ttctatctaa ataaagggat
     1981 gacaatcagt tggcttatca cttttggttt gatgggggcg gcatacggct ctatctacgg
     2041 gaatatgcaa cagtttttag aaagtaacga cttaatgaaa cagatgttta tccaatctgg
     2101 cgtctcaatt gaaaaaaact ttacaagcac gatcatggtc gtcttgattt gtctagctgc
     2161 tattttacca attgctagca tcaatcgttt atttacagaa gaaacccacg gacgtatgaa
     2221 tcagctgttc gcaacaaaaa tttctaggaa ccaactttat tggaccaata tgttactagc
     2281 tattctattt gctatattgg caatcctgat tagttctggt gcactagggc tttctgcttt
     2341 ggcttcgatg aaggatgcga aagacatgga tctattgatg tttcttaaag ccgggttcaa
     2401 tttcctgcct gccgttttag tgtttatcgg aattagtggc tttctattcg gatggcttcc
     2461 tcgaatgggt aaacttgctt atatctactt agcttactcc tttgcactca attactttgg
     2521 cggaattttg gatttaccag aatggttttc caaaacagct gtccctagct gggttccacg
     2581 gatgccaaac gattcttttg atctttccgt gttccttacg ttatcatgta tcagccttgc
     2641 actgttcgtc cttggttcta ttggttatag gcgaagagac ttactagaag gataataaaa
     2701 aagcatgtac aatttcatgc tagcctccaa atctgggtca aaaatccaac atttggaggt
     2761 cattttttta tttacaagtc ttctaactgc tctttatcaa tgatccagac ttctcgattc
     2821 tttcttttta cataaccatt ctcctctaat aatttaaatt ttctagataa agtttcgggg
     2881 gtcgtaccga taaatgcagc aagttctttc attttcattg gcaggaaaaa gtgatccttc
     2941 cctgtcgctt tagctaagtc aagcagatag acagcgactc gttcttctac tttttccatc
     3001 atcaaaaaag ttgtctgctg ctgtactttt gttaatttct caacgttgat tgctaaaagt
     3061 ttcagactaa gggcgggata atgcagtaag agttccgaga aatctttttg tctcaatatg
     3121 caaatttccg ttttttctag tgcttcgcca aataaagact catttggaac gcctagcaac
     3181 tggctctctc cctcatatcc gcccggctct attactcgta aaagctgttc ctttccgcta
     3241 gcagataatt gatacacttt catactgccg gaggccacga tcactaattg aggttctcca
     3301 ttcggcgcaa gaatctgttc tcctttttcc acatagtgat gtttgacaag tgcgtgtact
     3361 ttttcttgat cttgctgtac taagtgattg aacaaaggaa caaatgaaac acataaatga
     3421 tcatgactca taatctctcc tccttctaaa cagctatttc taataaagaa acataacttc
     3481 ccttattcca actgataatt ttcttttatc atagcataaa cgtcaagcga accagatacc
     3541 agcaaataga gtggatttct ttttgtccct tgctttattt tccttctttt gagaccttaa
     3601 gtaattgatt cctatcaatt ttttaaatat ttactttttg aataatcaaa aacaaacctt
     3661 cactcgcaag ccgagagtaa ggtttgtttt tcgcattgct tatttcgtgt tgtctattga
     3721 aatttttccg cacctagctt ttgatatagt ggtgctagtt cttgtaaggt ttcagcggtt
     3781 atttgctcaa cgtgttcgat ttcttgcgga tagaagaata gtttacccat ttgaaaggtt
     3841 cggtctctca tcgctttttt caactcttct tgtttctttg tatcggacaa atagtcagac
     3901 aaagctaagt ggtcgtcaag ttcatgttct ggttgcggcc atatgtaata atccttgata
     3961 tcgactgttt tgctccactc tttcagtatc gcaagcaact ggttatacgc tgcaatgcta
     4021 ccatatcgtt cttcgctttt ataatgttgg tacatcaaat agatttggta ttgtttttga
     4081 ttcaacaaaa ctgctagaca ggcattctcg tcttgacgag attcgcttcg gtacgcgctc
     4141 caaaaatgac tgcgtaaatt ccagccattc gtccagcttt cgattttagg tttgctcatg
     4201 ccgtacatat caggcaaaag tgcagctgtc tggagctgaa tctctttcca tttttgccag
     4261 tgttctttat attctttctt gatttgttca atcttatctg ttggatggct ctctttgagc
     4321 tgtttgaatg cgtagattgg tcgatcaaac aactgataat ctgattttcc aagttccatt
     4381 gtttcgcctc tttccgttat cttttgtatt ctacctgaat tttgcgatat ggacaaatag
     4441 aggttgtgaa aaagctgttc tgcatcaagt cataagaaca gccaaacaaa aaactgcgat
     4501 gagcatcacc gcagttttat tgaacttaag gttgattgat cacatctaaa agcgttgaaa
     4561 ataaaatggc actagaagca gctcctgggt cgatatgtcc gattgcacgc tctcctagat
     4621 aggaagctcg tcctttttta gctaccaaga ctttggtgtg ttcttttgcc tggtcaatga
     4681 tttcttttgt taaattccct gccttcaatg cctctgtgac tggcagccaa acatctaaca
     4741 tcgttttatc gtcagcttct gcctttcctc ggctttgaat acccgctgtt ccctgctcga
     4801 tcacttcgcc taatttttct tgagaatcaa tagaatcgac ctcttttgtc gctttcatca
     4861 tgttcatgaa tgcagatccg tataacggac cagaagcccc accgactttc gatatcatcg
     4921 ccatagagaa tactttgaat gtatcgctga ttgtttcagg atgatcggct tgaaaagctt
     4981 cttcataagc tgaaacccct cttcccatgt tatttccatg gtctccatcg ccaatcgggg
     5041 tatctagatc actgagatat tgtttgttct gattgattgc ctctgcaaat tgcgtgagcc
     5101 attcttggat ttcttgaacg gttagtttca tattatcacc cttttatctt gagatttttt
     5161 accatgaaat cgtctgtaca ttgctttcta aagaatcttt ccattgtgtc tccgtcaagt
     5221 cgatcagtgt tagagaaaga ccttgcatat ctagtgaggt cataaaatta ccgacttttc
     5281 ggaaaactac attgacgcct ctttcttcca acagagctaa aacatcattc atgaacacga
     5341 actgttccat caaaggcgtc ccgcccattc cgttgactag tacacctgct tcttttggta
     5401 aattcgagta atcatctaaa atcttagtaa caagttcctt tgccagtact ttagaaggct
     5461 gcatcttttc tcgacgatag cctggctcac cgtggatacc aacaccatac tcgatctcat
     5521 cttctcctag ttcgaatcct ggtttcccta cttctggtac agttgctgct cgaagcgcta
     5581 gaccgatcgt cttagttgcc tgtacgacct tttctcccag ttctttcaac tcagctaggg
     5641 aagcaccgtt tcttgctgca tcgcccacga ttttgtgtac aaggacagta cctgcaactc
     5701 cgcgttttcc agcagtatac gtactatttt caacggcgat atcatcatct acgatcacac
     5761 tttctacttc aatatcgtcc atagcagcta attcctttgc catatcgaag ttcaatgcat
     5821 cccctgtata attcttaacg atcagcaaaa ctccttttcc actatccgct tccttaatag
     5881 ccgtctgaat ctgatctgga gttggagaag taaacacatc accaagcacc gctgcactta
     5941 acatcccttc ccctacaaac ccggcatggg atggttcatg tccgcttcct ccaccagaaa
     6001 ccaaaccaac ttgttccgct ttctgattct tggctacaac acgggaagat tctacacgat
     6061 gaacaagttc tggatagctt tttactaatc cttccagcat ttcttcgaca atattgcttg
     6121 gatcattcat gatttttttc aatttattcc catcctttct ttccggcatt ttgtgcttct
     6181 gttaagattt gctgagtatc atctgttata ccggcagtga tggctgctgc aaaagctcct
     6241 tccactaaag gcgcatccac cagatgataa cgttgctgct gctcttcttc caacatgtct
     6301 tttgccagtt ccgagttcaa taccgcgcta ccgatatccg caaaaacaaa ataactagct
     6361 gcatcgcttg attcattgac tgcttctacg attttcatcg ggtctgatcc gatctcgcca
     6421 tttattccgc cgagagaaaa aatttgcacc tcttcggatt tttgcatctg ctcaatcatt
     6481 tctttaatac catctgttac tttttgacta tgagaaataa gcaaaatact ttttttcaca
     6541 agaatcttcc tttcttttac atagttgcta acgattggtt tcgctttcat taatgaacaa
     6601 ttcaagtata taataaattt ctaattttgg gaagatatgt gtagtgcttc ttgaagtttg
     6661 ttatacttta attaaaagag aggagtgatc gaatgattac aacaacaacg aacagtgtag
     6721 agggaaaagc cgtagtagct tataaaggga tcgtttttgg tgaggtcatt actgggatcc
     6781 atgcatttaa agacttggag gcggggttgc gaaatatctt tggcggccgc tcaaaaagct
     6841 atgaaaacga actaatgggt gcaagagaag aagcacttgc cgaaatggcc gaacgtgcaa
     6901 aagcattagg agctgatgcg atcattggcg taaaaatgga ctatgaagta ttaggcagcg
     6961 acaatggcat gctgatggtc acatgtagtg gaacagctgt ctccacgaga taagtagggg
     7021 acatctgttt taataactaa agagagatgg gacatgtagt ggcactcaag aattagattt
     7081 ttcagtctaa cttcgaggta ctctaccttc ccatcctctc ttttttattt ctttttgcgg
     7141 atgattccgt aagctgctgc gactaacaca cccgcccctg ctaaggaata actgacagaa
     7201 gctgtttttt catttgtttt tggcaaagtc tgacttgttg atttggatgg ctctgtcgtt
     7261 tgctgaactg tttctgtcgt gcttccagag gtcactgatt tttctcctgc cgaatgcaaa
     7321 atatctttta ttcgttggaa actagctgct aagtcagctt tttttgcttc aaaatcatta
     7381 gcaactgttt gtgcttcgct gatctttccg tggattgctt gactggccgc tgtgatatcc
     7441 gcttgagttt gacttagctt tccagaagct gtttgagcaa cctgatttaa ttgctgattg
     7501 aattcttttg cttgttggat cttcccgtca agatctgttt ggacagcttc cgcttttgct
     7561 ttcgcttcgg caagcttttt ctcaagttgt gcacggtcat ttcccacttt atttttaaga
     7621 tcagctaaat cttgatcgat tgctgcttgg tcttctttca atttgttttt tgcagcttct
     7681 aatgcttctt cgagttcttt tcttttattt tccaagtctt gcaaatgatc gatttggtca
     7741 gaagaggtag aactatttat tgatgtatct tctgaagatg aaatcgtttc tcctgaatta
     7801 tctgtcggta cattggaagt catttctgtt tccgaggcac cgttttcagc accagaagag
     7861 cttatagttg ttgaaggttc tgtttgagca ggttcagaag tttccgcaaa agctgtctgt
     7921 cccaataaga aaatactgga aacagcaatt ggccagatta acggcttcat tctttttttc
     7981 atcacttttc ccatccttac tactttttaa ctaatttctt attataagca aaaatgtttg
     8041 gaagaaccat aaacacaaca ggctgtttgt ctaaaaaacg gtgcatcttt tatttttttg
     8101 tacgttccgg gcttttccat gtcccgcttc cataacctaa aatcgttttg actgctggaa
     8161 tgaaagtcgt cacgatcgtt atcgcgttca ctatccagaa caaaagcata tatagtggtg
     8221 cgaaaagaag gtatttcaat tttcttcgtc gatcatctac aatcagcgaa gctagcaact
     8281 ggaaaaatcc tgcgaccatt tcaaagcaga tgaacacaaa actgaatgta aaagtaatat
     8341 aaatctgttc gtagtttcct tgatatacat aatgcccgat taatccagca aaaagaacta
     8401 cggaaataca aaagaatatt gaccagacaa tactcaatgt ttgatcaatg aagatgatcg
     8461 ttcttccaat atgtttaaaa ggatggagca tcactttttt gaaattcgtc agccagactt
     8521 ctgttccacc ttttgcccaa cgttttcttt gtcgatatag catcttcaac gtcacaggta
     8581 cttccatgaa aaagataatc tcaggagcaa aaacagataa ccagtcattt aactgatgat
     8641 cccaagcaat actgatgtct tctgtcgctc gatcttgtct gaatccgccc acatcgatca
     8701 aggcttcttt tcgatacatc gtatttgctc cactgtacgc ataaagacca ccgaaaacag
     8761 ctgactgtgt tcgtttaatg atgcccacga tacttgagaa ttcaaccgtt tgagattttg
     8821 caatcaattt ggtacgattt tggacatcca tatttgcagt gaccgcagca atatgtcttg
     8881 caccagggcg aatgaaataa ttcacatacc taatcagtgc atctggctcc ggtaccgtgt
     8941 ctgcatcatt gcttaaaatc aatttccctt ttgcaaaggc aagtccgata ttaaacgcgt
     9001 gggcttttcc tttatttttt tcgatccgaa ccacacgaag attagcgtat tttttcatca
     9061 gtctagctaa tatttctggt gtttggtccg ttgaaccatc gtctgtcact aatacctcat
     9121 agttgtgata attcaatttc gtcatcaaat actcgattgt atcttcgatc acaatttctt
     9181 cattatgggc tggcaccatg atcgtgatga acggttcgac actcaaggga acatctaccc
     9241 attctttttg cttatgtttg tataaaaaca cataacacca gacaccaata aaccatacaa
     9301 atccgcctaa aatcggataa gaaacgagaa aaaatacata atagacaaac aagtatctaa
     9361 gaataaatta cctgtatggg tgattgagat catttcagtt ccaccccatt ttctttatac
     9421 aaatctgcaa tgaatgtcgt atctagattt ttctcttcgg agacagaata aaaacggacc
     9481 gtttctctct cttcttttgt tccaaaacgt tccgtataca cttcttcaag taattcttgt
     9541 ctttttttca atttttcatc ttcatattca aaa
//
LOCUS       DARDHA010000105         9506 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000105 DARDHA010000000
VERSION     DARDHA010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.106"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            26..2440
                     /locus_tag="SDA10_002006"
     CDS             26..2440
                     /locus_tag="SDA10_002006"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEG4240897.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            2788..4341
                     /locus_tag="SDA10_002007"
     CDS             2788..4341
                     /locus_tag="SDA10_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEG4240898.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(4722..6506)
                     /locus_tag="SDA10_002008"
     CDS             complement(4722..6506)
                     /locus_tag="SDA10_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4240899.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(6521..8242)
                     /locus_tag="SDA10_002009"
     CDS             complement(6521..8242)
                     /locus_tag="SDA10_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4240900.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            8342..9103
                     /locus_tag="SDA10_002010"
     CDS             8342..9103
                     /locus_tag="SDA10_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG4240901.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         3082 a         1694 c         1914 g         2816 t
ORIGIN      
        1 tttcaataga acaggaggac agcaaatgag ttacaatcat aaagaaattg agaaaaaatg
       61 gcaaaaatac tgggcaaaaa cgaatacttt caacacccat gatgatcctg aaaagccaaa
      121 attttacgca ctggatatgt ttccctatcc atccggtcaa gggctacatg tagggcatcc
      181 tgaaggttat acggcaacgg atatcctttc acgtgtgaaa agaagtcaag gatacaacgt
      241 gctgcatcca atggggtggg atgcattcgg tctgcctgct gaacaatatg cgctagatac
      301 aggaaacgat ccagctgagt ttacccaaaa aaatatcgaa acattccgac gccaaatcaa
      361 ttctttagga tttagttatg actggaatcg cgaagtgaat acaactgatc cggagtatta
      421 taaatggaca caatggatct ttacgaagct ttatgaaaaa ggtctagctt atgaagcaga
      481 agtagcggta aactgggtac ctgaattagg aacagttatt tcaaatgaag aagtgatcga
      541 tgggaaaagt gaacgtggtg gctatgatgt agtcagaaaa ccaatgcgcc aatggatgct
      601 gaaaatcact gcttatgctg atcgcttatt ggatgatcta gaattagttg attggccaga
      661 aagcattaaa gaaatgcaaa gaaattggat cggtcgttca gtaggagcaa atattgaatt
      721 taaagtggct ggtacggata aatcctatac cgtatttacg acacgaccag atactctatt
      781 cggtgcaact tactcggttc ttgcacctga actagatctt gttagagaaa ttacgacccc
      841 tgaacaaaaa gctgctgtag aagcttatat cgaagagaca gctaaaaaat cagatctaaa
      901 gagaacggat cttgcaaaag aaaaaacagg agtatttact ggtgcttatg caatcaatcc
      961 agttaatggc aaggaaatcc caatctggat cgccgattat gtattatctt catacggcac
     1021 aggagccatc atggctgttc cagcacatga tgaacgtgac tttgaattcg ctcaaacttt
     1081 cggactagaa attcttccag ttatcgaagg tggagatgtt gaaaaagctg cttacacaga
     1141 agatggtacg cacatcaact ctgaatttct agacggcatg aacaaacaag aagcaatcga
     1201 taaaatgaat gaatggctag aagaaaatgg cgtagggaaa aaagaagtca gctatcgctt
     1261 acgtgactgg ctattctccc gtcaacgtta ctggggtgaa ccaatcccaa tcatccattg
     1321 ggaggacgga acagtcacag ctgtcccaga agaagagcta ccattacggt tgcctaagac
     1381 aaaaaacatc aagccaagcg gtactggaga atctccatta gcaaatatcg aggagtgggt
     1441 caatgtcgtt gatcctgtaa caggtaaaaa aggccgtcgc gaaacgaata caatgccaca
     1501 atgggcagga agctcatggt attacctgcg ttatatcgac cctcataaca aaaaagaact
     1561 agcagattat gaaaagttga agcgttggct accagttgac atctacattg gtggagcaga
     1621 acatgcggta cttcatttac tatatgcacg attctggcat aaattcttat acgatatcgg
     1681 tgttgttcct acaaaagaac cattccaaaa actatacaat caaggaatga tcttaggcga
     1741 aaacaacgaa aaaatgtcaa aatctcgtgg taatgtagtc aatcctgatg atgtggtcga
     1801 aacttacggt gcagatacac tgcgcatgta cgaaatgttc atggggccat tagatgcttc
     1861 gattgcttgg agcgaaaatg gcttagaagg aagccgtaaa ttcttggatc gtgtatggcg
     1921 tttgatcgtt gatgagaaca ataaaatgcg tgaccgtatc actacattga atgatggcaa
     1981 gcttgataaa gtgtatcatc aaacagtgaa aaaagtgaca gaagactatg aaaatcttca
     2041 tttcaataca gcaatctccc aattgatggt ctttatcaat gaagcctata aagtggacgc
     2101 tctgccttat gaatatatcg aaggatttgt tcaactactc gcaccgattg ctcctcacat
     2161 gggtgaagaa ctatgggcaa tccttggaaa tgatggtggt atttcttacg caccatggcc
     2221 aacatacgat gaagcagcac ttgttgaaga cgaagtagaa gttgttttcc aagtaaacgg
     2281 taaagttcgt gcgaaaagca atgttccccg agacttagga aaagatgagc tagaaaaagc
     2341 agcattagcc aatgaaatag tgcaagaata tattgaaggc aaaacagtca gaaaagttat
     2401 tgcggtacca aacaagttag tcaatatcgt tgctaattaa ttggctcttt gtcaataagg
     2461 actgatgagt tgtgcaaaat caaatctggg ccagaatgaa cttcattctg tctcagattt
     2521 tttgttattt caaattgatt aattgattga tcaaaaaggt tttgattttc atgttctcaa
     2581 acacgttcaa tgtctttttc ttctgaatgg tcttgtaaga gatctctgta atcatcaaat
     2641 ttttgtctat taatcttagt attttttgat gtataaacta cctgaaattg agagtacata
     2701 ccaaattcat aaatttttta ttagtgggac tagtgattgt atggattttg acgcataatg
     2761 aatgaagtat gagaaaagga gagacaaatg gatcaagaac tatttattat cgaagtatta
     2821 tcagctaaac aaaaaattat cgagaaagcg aaaaaaagag cgaaaagaat aatgttcaag
     2881 cttttaagct tagtattttt ctctggtttg ctttttttcg gagctgtatc gttcctatat
     2941 cgccatatga gtgcagtagt ccgttcagct gaagggatgc aaaaattaaa taatttgtat
     3001 cacttttcgt ggggagttgg cggaataaca attgttgtgc ttattgtttt tcttctactt
     3061 atttggctcc gttaccatca attgtcgcgt catttcgatc aatggcttag tcaaagagca
     3121 caacagtttt tacataataa accaacttct caagatgatg actatcttta ttttgagaag
     3181 acttcgacga gtcaagcttt tcggatacaa aaaagtgatt gccagttatt gctgagatca
     3241 cttgatgatt tacctatcta cttaggcgaa agcaaatcaa aattcggaat gaacacattc
     3301 catatttttt tactagaaaa cgaacccaga tgggaaaaaa gccagtcttc gcctggtgga
     3361 tacctgacaa ctaagatgag tacattagtc actggttttt tcctcattct tttagctgga
     3421 acaatctatt atgggagata cactcagcca aaaatgtctt ctcatataaa tgctgcttct
     3481 cacatgacaa tgaactcaac taacgatacg catatacaaa agacaaatga agggttgtta
     3541 gctgatccta agcaaaaggt aaatttgaca acacaaaaga taaatgatct caaacttgat
     3601 acagagacgc actctctcta tatgacaaca gatagtggtg ctaattgggt atttgtgccg
     3661 attgattcgc aatggttgcg ttttggcgaa tacaccctga caacggggct gattcctgaa
     3721 ggttattgga tggacaacac atatgatatt tccgccgatt tctcatggtt tatctattct
     3781 tctggggatg atgtacgtct gctgacttca agagataatg gtaaaacatg gcaaacgtct
     3841 attatcaccc aatcagttca gccgatacgt tatcgaaaag cggtatttta tggcaacgat
     3901 ggtctattta tctgtacatc agccagtggg atgtctgcag aaactttgta catttacact
     3961 actatggatg gcggaatcac ttggataaaa agtggaagta caatgatcaa tcagccagta
     4021 caaaatgtca gcttcgttac aacttcttta ggttttgttt cgacacgtga aagtttgttt
     4081 tatacaaaca acggaggacg ctcattcaaa gaatcagttc tgtcgattcc ggaaaactat
     4141 cagacagggg ggatagatct ttttcaatca ccaaatgaag ttactcaaat aagcgcgaat
     4201 acgttagaga caaaatttta tttgttgaaa aaaggaccaa tcgatgcagg gaaaatgttt
     4261 gcctgcctct tccgttcaac tgataacggg gaaacatggc aattcgttca gcaactatcg
     4321 caagtagaac aaggagatta aagaagagag attgtgagcc tgacgttcag caccgagtca
     4381 caagaaacaa attcaaaaaa cagcttctca tatttgttga atagtgtaac tcgtgattcg
     4441 gagttgacaa gagcaacaag aagcgatttt gaagtatagc ttctcgtatt ttacaagatc
     4501 gtgtcttatt gccgataggt tgtctcttga acaccgttta ttcgtaaaaa gggattgtga
     4561 caagtctttt gtcactactt accgaacgaa actgaaggag ctatccaaaa agtatcatta
     4621 gtattattct ttgtgtttct agtggcacca ctagtacatt taaaaaagct atcgatcatg
     4681 gactaatcat acatgatcga tagcttttta gataatgttt gttacgctat ctgaaattga
     4741 ctattataaa gactggcata aagcgttggt gattggagta gttcagaatg tgttcctttt
     4801 tcgacgatat cgccattctt cattacaagg atcatgtctg cattttcaat cgttgacaaa
     4861 cggtgtgcaa tcacaaaact tgtcctatct tttgtcacag cttccattgc ttgctgaatc
     4921 aatgcttctg ttcgtgtatc tacgctagaa gttgcttcat ctaaaataac gacaggagga
     4981 ttcgctagaa tgactcgagc aattgtcaat aattgttgct gtccttgaga taacgtgccg
     5041 ttttcgctag aaatgatcgt atcatatccg tctggcagtg ttcgaataaa gtgatcacat
     5101 tgtgcgattt cagccgcttc gataatttgt tctcttgtgg catccatttt cccgtaggcg
     5161 atattatcag ctaccgttcc ttcaaacagc caggtatttt gtaaaaccat cccaaaagaa
     5221 cgacgtagtt ctccacgtga aagcttggta atatcaagac cgtcgaattc gatctttcct
     5281 ttattcagtt cataaaaacg catcagcaga ttgactaaag tcgtttttcc tgcaccagtt
     5341 ggaccaacga tcgctaccgt ttgttttggc tttactgaaa aatcgacttg attcatcaat
     5401 attttatctg atgtgtaacc aaactgtaca tttttgaaag ctatttttcc tttcggttgt
     5461 tctaaaagtc ttgcatctgg aaggtctgga atttcatctg tttcatctaa tatctcaaat
     5521 atcctatcga ttgctgccat tgcggattgg atcgagttga tcacataaga agcggtagaa
     5581 attggttcag aaatctgatt gatgtactga agataagctt gtaagaagcc gatcgtcaca
     5641 ccacctgata gaacaagcat agcaccgaat accgcactta tgatgaaggc gagttgatta
     5701 atgaaacgaa tggccggata gatagcacag ttcataaatt gagcacgttt aaaagcgttg
     5761 tattgctttt tatttacttc gtgtatagca gcttctgcat gagcttgctg attgaatgtt
     5821 ttgaccacta gattaccagc taagatttct tccattcgat tatttaaaac acccagttca
     5881 tactgattct gttcagaaat ctgtttgttt ttgttcgcaa tcttagttgt aacaattgaa
     5941 ctgccaacga tcagcaataa gacgagcaaa gtaagcttgc tttcgatata aaacagcatc
     6001 agaccagcag cagctattgt tacgacagaa gtaaaaaatt gattgatacc tgtcaataac
     6061 acttgagaaa gttgattgag accggtagtt gtccgactaa tgatgtcacc tacttgatga
     6121 ttgtcaaaaa acgacatcgg taatgttttg aatttttttg tgatttcttt tcgcaccctc
     6181 aatgtgatct tttcgcttaa cgctgccatt gtccgttcct gaataaatga agtgatgctg
     6241 cttaagactg aacagccgat cagcaataag acagggaaca acaatgcttg tttgatatca
     6301 ctgaaagaaa gctgtccaat acctgttttt cttatttctg ataagagatc atcgatcccg
     6361 attcccatga tcaagggcat ggctacgact aataggttcc cgaataaact gcatagaaac
     6421 aatccgtaaa aatatttatg ttcaggtttg atcattttcc aaaaacgtat caaagcacgc
     6481 gtgttcaacg tttttttctt ttccatcgta tcccttcttt ctatattcct tgtgattttg
     6541 caaactcctg ataagtttgg caattagaca gtagttcttc atgagtacct tgtccaacga
     6601 tttttccttc atctaaaaca atgatgttat ctgcttgttg gattgtactg aggcgttgag
     6661 caacaatcac gagagttttc tccgacatgt gtttatgaag ggctgctctt aatgctgcat
     6721 ctgttttata gtctaaagcg gagaaactat catcgaatac atagatctct gctggtttga
     6781 tcaacgctcg agcaatacat atccgctgtt tctggccccc tgaataatta gaaccgcctt
     6841 gagcaacaaa gctgtccaat ccgtcaggaa gggtgtccac tagttcttct aattgagcga
     6901 tcttgatcgc tttagacaac tcttctgttg aggcatattc atttcccatc tgcaagttgc
     6961 ttaaaattgt cccgctgaat aagaaagcct tctgagggac ataactgatg tgggagcgga
     7021 tctgttcttg agtcatttga gtaagaggaa taccaccaag ggttactttt cctatcgtag
     7081 ggtcactaag tctcaagaga agtttcgcca aactactttt tccagatccg gtcccgccaa
     7141 cgatcgctgt tgtttttcct ttaggtattg tgaaatcaat atcttctaga actggatcag
     7201 agatttcagt atatttaaat gtgacatgtt caaaagcagc tgcatttgtc tgatcaaagg
     7261 ttggttcagc tactgttttg atcgtctctt cctcaatttc gatctctgct tgcagtattt
     7321 cttctatccg tcgtaatgag gcaaacgatt tagggataat gaccaatacc atagctgcga
     7381 tcatcaagta agcaagtgtc agcatagagt attcgactac agcagtcacg acaccgattt
     7441 gtaatgctcc ttctgctaca agatttccgc caaaccacaa aatagaagca taaacgattc
     7501 ccatgagcaa aaaagcaatt ggagtgaccc aagcaaatat tcggttcact ttgatcattt
     7561 gattagcata gtcagcaaag ctcttattcg ttcgcttttc ttcatatgat tgattatcaa
     7621 aggcacggat catattaatc ccagtaaaga attctctcaa tgtgatggtg atccgatcca
     7681 tttttggctg gatactcaaa gataaaggtg cccctcgttt catcagtcga taaacaatcc
     7741 atacataaaa aacaacagag atcaatgtaa cccatgttag taatagagaa taacgaaaca
     7801 tcatgagaat ggtgaaaacg caaataaccg gtgctggtaa gatcatttgg caaaataaaa
     7861 caatcatttg ctggacatta tcgacatcat tcgtcattct tgtcaaaaga gatccagaac
     7921 taaaacgact gatatccttc attgacagct gttggaattt acggaaatat ttctcccgcg
     7981 tttggaatcc aaacctagcg gaagccaatg ctgaaaagta actagaggca actgcagcga
     8041 ttgttcctag aaacgctaca agcagcattt caactccaat tttttttata actgaggctt
     8101 cgccacttgc tatacctgtg tcgatcagct ttgctactaa ttttggtaca cctaatgtac
     8161 cggcgatttc taaacagatc atcacaaagg taaggagagc gagtcctttg tttttctgta
     8221 agaatgtttt taagatagac atattattcc tccttttttc ctgatacttt tcgattctat
     8281 accttcaagt aacttgaagg tcaatagaaa aaagaataaa ctgaaaaaga ggtgagaaaa
     8341 aatgaaaaaa atgtatacga ttggccaagt atcggaacta tttgatttgc caaaatcaac
     8401 gatccgctat tgggatgaac aaggattaat ccgttcaagt agacaagaag agaatgatta
     8461 tcgattgttt gatatcgatg atatttttat gctttatgat atcgtttttt atagaaaatt
     8521 agatataccg atcaaacaga tgaagaattt atatggaaaa acattgacgg agttatatga
     8581 gacacttgat gaaacagaaa gaagaatcca taaagaactg gtggtgatga aacaaaaaca
     8641 aaaagagata agggagcaaa aaaaacagtt gaaattgatg atcgatacaa acgaggaaga
     8701 atttcctgta gaagagatcc cttttgactg tatgatctca acggagtttg aagatattgt
     8761 ggaaatcaaa aaatttctac ctaactattc aagctttggg atgatgagta tgccagcttc
     8821 cagttcacag acgatgtatg gattttttat cgaccctagt gaagtacatt tatttactca
     8881 agacgtgatt tgggagaaaa aagatacggc tgtttatcgt cgattcttat tgaaatccga
     8941 gatgaaccat gcggaacgta ataatatttt agaaatcaga gaaagaatgt atgaaaaagg
     9001 atggaaaaca ggagaagtga tcggccagta tctattgacg aatacggatg aaaacaatat
     9061 cagaactgaa tattatcatg catggataga aatgaaaaaa taggaaatat gtgtttaaga
     9121 taactcgaaa atgatggttg ttttagttat aaagaaaaat tttctggttc gattacgggt
     9181 ttaatgtggg ggattattag tcgtggatag atagaatttt agttttttag aggaaatatg
     9241 tctttaaaaa tggaatggtt gcaattctta gcgatatata cgatgtaatt gtcctcttaa
     9301 aaaagtatgg aaaaataatt gtattgggtg ttatgtataa ttcatggtga actgagacta
     9361 atgagaagaa aaattcatat tgttatgagt gagattgtat cagatgagaa tgataaatta
     9421 aataggtgca taaaagcatt acacttaaaa tacattgttg cttacattat tacaatatgt
     9481 taataaggca aaaataaata gatgga
//
LOCUS       DARDHA010000106         9452 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000106 DARDHA010000000
VERSION     DARDHA010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.107"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            210..410
                     /locus_tag="SDA10_002011"
     CDS             210..410
                     /locus_tag="SDA10_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG4240902.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            557..3346
                     /locus_tag="SDA10_002012"
     CDS             557..3346
                     /locus_tag="SDA10_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG4240903.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            3393..3920
                     /locus_tag="SDA10_002013"
     CDS             3393..3920
                     /locus_tag="SDA10_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEG4240904.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            3941..5131
                     /locus_tag="SDA10_002014"
     CDS             3941..5131
                     /locus_tag="SDA10_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG4240905.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            5171..6469
                     /gene="asnS"
                     /locus_tag="SDA10_002015"
     CDS             5171..6469
                     /gene="asnS"
                     /locus_tag="SDA10_002015"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEG4240906.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7645..8319
                     /locus_tag="SDA10_002016"
     CDS             7645..8319
                     /locus_tag="SDA10_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG4240907.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            8316..8657
                     /locus_tag="SDA10_002017"
     CDS             8316..8657
                     /locus_tag="SDA10_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240908.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            8687..9046
                     /locus_tag="SDA10_002018"
     CDS             8687..9046
                     /locus_tag="SDA10_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEG4240909.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
BASE COUNT         3159 a         1691 c         2042 g         2560 t
ORIGIN      
        1 tcggtttttc actttatgta atcgctgaga aaactttttt taataaaaaa agtcgaaaaa
       61 ctctagcttt tttgtaaacg gtatgatata ctataggagt agtttttgtt cggtaatctg
      121 tggtagcttg tttcaaataa acatcttcca gatatacacc attcaagtaa ttgcaaaata
      181 tgtgttgata cacaaggagg atttttatta tggaaacagg tacagtaaaa tggtttaact
      241 cagaaaaagg atttggattc atcactgcag aaaacggaaa cgatgtattc gttcatttct
      301 cagctatcca aggcgacgga ttcaaaactt tagaagaagg ccaagcagta actttcgatg
      361 ttgaagaagg ccaacgcgga cctcaagcaa caaacgttac taaagcttaa ttaacctata
      421 ctaaaaaata aacgcccaga catttgcgtg tctgggcgtt tttgtctatt ttctttaaaa
      481 agcgatgaga gtatttttct gcactttgta gtataatttc aaaagaacat tagaggcagg
      541 agaagaggaa gatattttga aaccaagaac ttatgcagtc gttgatatcg aaacgactgg
      601 gacgaatcca aaagaagatc ggatcatcca atttggctgc gtcatgattg aatcaggtcg
      661 gatcacttca cgtttttcga tcgatattca tccagggcgg aagatttcaa aacaaatcca
      721 gcatttaaca gggatcacga atcaacgcgt ccaaaaagcg ccttatttcg aagatgtcgc
      781 ccaaacgatt tacaatcttt tggcagacac catatttgtc gcgcataata tttattttga
      841 ttataatttt ttgaaccatg aactcatgcg atgcggactt cctagtttga agataccagg
      901 catcgataca gtagaacttg cacaaatttt cttgccgacg gaaccaagct ttcgcttagc
      961 tgatttgtca gaaagccttg gatttattca tgagaatcct catcaggcag atagtgatgc
     1021 cgaagtgaca ggtcaacttc ttttgctgat cgaagaacgg atgagaaaat tgccaatcat
     1081 cacgttagag cagattgcgc gattaagtag acacacaggt atggatacga gtcgtttcat
     1141 ttatcatgtg attgaagaaa tgaaagaaaa accagagcca cttgatccct cattagaaat
     1201 cgtcgatggg ctagctttgc agaaaaaaga agtggaacta ttcacatccg tccattatgg
     1261 cgagcggaca tatccaagaa aaaaacaagc aaaagaaaaa atgtttggta aaacactgat
     1321 gtatcgaaaa gaacagaaca gactgatgaa tgcggtctac gaccatttca caaaagatga
     1381 atcaaaagat ttgatgatcg aagcagctac aggaatgggt aaaacaatcg gttatctgct
     1441 gccattgagt tatttggcta cacctgaaaa acccgctgtc atcagtaccg tttcgctagt
     1501 tttgcagcag cagatcatag aaaaagacat tccattgctg aatcaattgc tagatcagcc
     1561 catacaacct gtaattatca aaagctatcg ccactatatt gatttgcagc gttttaaagg
     1621 cacacttgtg gaaccgccag aacaaaaaca atatgctttg tatcaaatgg cggtcttagt
     1681 ttggctgacg caaacgaaga ctggtgattt ggatgaatta catttaacaa atttgaacca
     1741 ttcattcttt gcagatatcg cgcacagagg tattggattt ttagcaagaa atcagtcatt
     1801 ttacgaacaa gactttgttc gtttcttgca aaagaaaatc aggcaaagta attttttgat
     1861 tgtcaatcat gcgtttttag tacaagaaac acagcgaaaa gaaccattgc ttccagctag
     1921 tccatttttg ttgatcgatg aagtacatca tttgccggaa atcgctgaaa aagtaaatgg
     1981 caaacgatta agtattaact atttccataa acaaatccag caattagaag aaccaaatca
     2041 gctgtttgac cgaatacaaa gctatttacc gaaagaacat gaagccttac gctggttatc
     2101 gatttatcga gaagaacttt ctgcgctatg cgaagcacag atttgtatgg cagaaggatt
     2161 aatgagttta gtgagcggca agcaacctaa cggacagtat ccagaagaag tcatgatgac
     2221 aaaagagatg ttggatcagc tgccactgga atctgggaca gccattgatc acctgcagct
     2281 tttttatcag gagatccagc agctacagtc acaaatgcgt ctgacttttg agattatcca
     2341 agaaagctgg acaaaccaga tgcggatcga tttagcaagt ttatatagcc tatttgatca
     2401 attagacaag caatacttgc tgatggatca atggctggaa gactggcgag atcatttgat
     2461 ccattggttc gttcctggga acacagcgca gcagatgatt tttcatctcc atgattttca
     2521 agcggctatg ttgccgtcga ctgtttggta tcctcgatat gatcgtattc tttatattgg
     2581 aggaacgctg aagatcggcc ctaatcgaca ctatctctct aagaggcttg gcattgaagc
     2641 agcacctctt aaagtgattg cctctcctta tgattatgag aaacaggcaa ggatctatgt
     2701 gccaaaagag gcacctgccg tccatcaagt cagcaatcaa gcatatatcg attatttaga
     2761 gacagcactt acaggtctga tcgaacaaga agatcggcca atacttgtgt tattcacttc
     2821 tcatgatgtt ttgcagaagg tctatcagaa aatgcatctg tctatgctag aaaaaggacg
     2881 agaaatacta gctcaagggt ttggtggaag ccgggagaag cttttgaaac gcttcatttt
     2941 gtcagaaaaa agcgtattgt ttggcgctga cagcttttgg gaaggtgtag atttgcctgg
     3001 agatgctttg cagatcgttg tagtcacacg tttacccttc gataatccgc ttcgcccgat
     3061 ggtcaaagct aggaacgctt atttagaatc agaggggatc aatccttttt accaggaatc
     3121 cgtacctaaa gcagctttga agttacgcca agcattaggt cgcttgataa ggacggagac
     3181 agatcgtggc gtattgctta ttttggatcg aagactagtc actgcaaaat atggcgcacg
     3241 gatcatccat gcattaccga aaaaagtacc gatcaaagag ctagctatgg aggaaatcta
     3301 tcaggatatc catgaattct taaagaaaaa tgaagaagga agttagagga gctgtctttt
     3361 ttcataatta aaatctgcta taatgaaaag gaatgaagat aaaggagcga cgacccttga
     3421 agaaaaaaca gacaaaggaa aaaacgatta atcgcgtgtt acttggcttg atcgtatttt
     3481 tattagcggt catcgtgatc agctcgattt tttacttgcg ttccagtcgt ccggcggtgc
     3541 aggcgaagaa agaagcagta gaaatcgcaa agaaatatgc aaatcttgag gaaaccgatc
     3601 atttttattg gtttactcga aagaagacat acttttcact gacaggtaca aatgataaag
     3661 gaaaagaaat cgccgttatc attcctaaat ctggtgaaaa agtaaaagta ttggatcaaa
     3721 caaagggact gactgaaaac gaagcaaaac agacaattgc tgcggcccat cctgaaatcc
     3781 aaatcgaaaa agctgcttta gggatgtatg atgatcagcc tgtgtgggaa gtagtaggaa
     3841 aagaaacaaa tgggaaactg aattattacc tgattggctt tgaatctgga gatgaagtca
     3901 agacaatcaa gggaatttaa aaggtaaagg aatgaatggg atggagttat caaaaagaac
     3961 aaaacgatta gaaccgtctg tcactttggc agctgcggca aaggccaagg cattaaaagc
     4021 aaaaggcaga gatgtgctga gtttgactgt gggagaaccg gattttgcga caccagaaaa
     4081 catccaagaa gcagcaatcg aagcaatccg caatgggaaa gctagctatt atacaccaac
     4141 agcaggaatc ccagagcttc gacaggcaat cgttgattat ctgaagaaaa actatggact
     4201 tgtctatgaa ccttcacaaa cagtcgtaac agacggagcg aaatttgctt tgtataccct
     4261 tttccaaacg atccttgacc cgcaagatga agtcatcatt cctgtgcctt actgggtcag
     4321 ttacggtgag caagtaaaac tggcagaagg acttcctgtc tttgtgaaag gggaagaatc
     4381 caatagctgg aaagttactg ttgaacagct agaacaagca cgttcagaaa aaacaaaagc
     4441 cgtaatcatc aattctccat cgaatccgac tggaatgatt tatagcaaga atgaactgca
     4501 ggcaatcggt gaatgggcag taaaaaatga tattttgata gttgctgatg atatttatgg
     4561 ccgtttagtt tataatggca atgagtttac tccaattgca acgatttcag aagcaatcaa
     4621 aaatcagacc atcatcatca acggcgtgtc taaaacatat gcgatgacag gctggcgcat
     4681 tggttatgcg gtgggaaatc ctgtgatcat caatggaatg atcgctgttg cttcccaatc
     4741 aactagcaat ccgacagcag tcagtcaata tgcagctgtt gaagcattga aaggtgagca
     4801 agatacagta gaagaaatgc ggaacgcctt tgaagaacgc ctgaatacgt tatatccgct
     4861 tgtggcagaa ctgccgggtg tttctcttga aaagccgcaa ggagctttct atctttttcc
     4921 taatgtaaaa gaaacgctac gcatgtgtaa gtatgaaaat gtcacacaat gggtagaaga
     4981 tcttttagaa gaaacagggg tagcgcttgt gactggcgaa ggctttgggg caccagaaaa
     5041 tgtccgcatg agttatgcca ctgatcgcgc tacgttagaa gaagcggtac gcagaatcaa
     5101 gcaatttatt gagagtaaaa gtcaaaatta atcctgaaga aattcaagga atataaaagg
     5161 agagtcatac gtggaacaaa ttcaaatcat cgattcgaaa aaccatgtgg gagaaacagt
     5221 aacaatcggc gcttgggtcg caaataagcg atcaagcgga aaaatcgcat ttttacaatt
     5281 aagagacgga acagcctttt tccaaggtgt agttgtaaaa agcgaagtgt cggaagaagt
     5341 tttccaaaca gctaaaaatc tacaccaaga aacatccgtg ttagtaactg gagaaatccg
     5401 tgaagacagt cgttcaaaat ttggttatga aatcggaatt acaaatgtgg aagtggtaag
     5461 cgagagccac gattatccga tcacaccaaa agaacacgga acagacttct tgatggatca
     5521 tcgtcattta tggctgcgtt cttcaagaca acatgcgatt atgcagatca gaaatgaaat
     5581 cattcgtgca acgtatgaat tctttaatca acatcatttt gtcaaaattg atccgccgat
     5641 cttgactggt tctgcaccag aaggcactac ggatctattc gaaacaaatt actttgatca
     5701 aaaagcttat ttatctcaaa gtggccaatt atacatggaa gctgctgcta tggctttcgg
     5761 aaaagtcttt tcattcggtc cgactttccg tgctgaaaaa tcaaaaacac gacgtcattt
     5821 gatcgaattc tggatgatcg aaccagaaat ggctttcact catcaagaag aaagtttaga
     5881 agtacaggaa caatatgtag cattcttagt acaaaatgtt ttagatcatt gcgaatatgc
     5941 attagatgta ctaggacgag acaaagaagt cttaaaaact tatacaaaat tgccattccc
     6001 tcgtattact tacgatgatg caatcgaatt actgaagaaa aatggatttg atgacatcga
     6061 atggggcgaa gactttggtt cacctcatga aacgttcatt gcgaattcat tcgaacagcc
     6121 tgtctttatc ctaaactatc caaaagccat caagccattc tacatgaaac cacatccaac
     6181 aagagaagac gtggtaattt gtgcagatat gatcgcacca gaaggttacg gtgaaattat
     6241 tggcggaagc gaacgggcaa cagactatga tttcttgcta gaacaaatcc gtaatcacgg
     6301 tttggatgaa aaagaatatt catggtatct tgatctacgt aaatacggtt ctgttccgca
     6361 ctctggtttt ggtctaggcc ttgaacgaac agtcacttgg attgctggta tcgaccatgt
     6421 acgtgaagct attccattcc cacgtttgtt gaaccgaatt tatccataag aggtgatgaa
     6481 aaagctgttc tgcatcaagt aattagaaca gccaagcaaa aacagctgtt catgtttttg
     6541 aattggcggg acttattacc gcagatgtaa gcttttgaat accgtttagt ggagaggttg
     6601 tgaaaaagct gtttagtttc gagtattaag aaaaattttg tggaaatggc tttccacatt
     6661 tttgcataaa tttggcttaa taccgagaaa ctggcttttg aacaccgttt aaggaagagg
     6721 ttgtgaaatc agtgccttga cctgagtatt agagaaacaa aacggaaaaa tagcttctca
     6781 tattttaccg tttttttagc taatatcgag ggtcagctga ttgaacaccg tttatatcaa
     6841 gaggttgtgc gcctgccgtt cagctccgag tcacaaggag taatttcaaa aaacagtttt
     6901 tcatgtttga tgactattgt gacttgtgac cgaggagttg gccggagagc aacgtttatt
     6961 taaaaaaaag aactgcgaac tctcgcagtt ctttttttaa aatacgggat aaaggaagtt
     7021 tccagcagac aagagaagaa agcaaaagaa attgctattt gattcacaag gttctataac
     7081 gaaatcgcaa tcaatcaaac atatacaaag aaggttattc aaatggaaat ctgatttgat
     7141 gatgcttctg ctgttgaaat aaagcagcat ctagtacctg aaaacaaact aatagtaaca
     7201 ttcgaagatg gagtaggtcc gtattttcag catgcaatga tccatatgta agtttaattc
     7261 atccttgaaa tcatcggacc agataaagtt atgaccaatc tatcccaagc aatctagaag
     7321 agatattagt aaaagaacat ttactggaag tcttagatga gcctataaat atatgttctg
     7381 atggcgtcat cagtggtctg attgacggaa gcttaggtgt gatcgatttc tctggaccaa
     7441 acaacgaagg aatcaagggg aatccggcac gttaagaaaa aaagtataaa aaactagaca
     7501 cttgatgagc gttcattcaa ttgattgaag ggactaggct ggtgtcattt tttatacata
     7561 tattttctct ttcgtcttga tagggcttat gggataatat aaggaagaaa atcatcgttt
     7621 tattcaaaaa ggagtttttg acaaatgaaa aaacgtatat tatttatcgg tgacagcatt
     7681 acagacgcag gaagagaccg agcagaagca actgatctag gtaaaggtta tccacttttg
     7741 attgccggaa aactaatctc ccaatatcca acgatgaaat ttgaatgcat gaaccgtggg
     7801 ataggaggag acaaaattgc agatctgcaa gtacgctggc aagaagattg tttagcttta
     7861 caacctgact atgtatcgat tttgatcgga atcaatgata cctggcataa tactagcgat
     7921 caggctatat tcggaactga gaaatctgcg cagcaatttg aatccgttta tcgaaaatta
     7981 ctaaatgaac ttcaaacagc ggaaatctgc aacatcatct tgatggagcc gtttgtactt
     8041 cctataaccg acgaccgtca tggctggcgt aaagacttag accctaaaat ccaaattgtt
     8101 cgtaaacttg ctaaggaata tggcgcattg ctggttcctt tagatggcat attcaatgct
     8161 ttagggattg cttatggttt ttcgaaatat acaagagaag atggggtcca cccaacgatt
     8221 ggcggacatg agatcattgc acaaagctgg atagatgcaa ttgccgcagt cgattttttt
     8281 agtggagata aaaatgagcg aaagaaaagt gagaaatgat ggaaatacgg tgggcagtaa
     8341 aagaagattt acctcaaatc aatcagctga ttactgatca tccagaatca ttcgtaaaag
     8401 caaaaacggc agctgaccta aatgataaga ataaactatt gctcgttgct gtaacaaaag
     8461 aaaaacggat tatcggattt gcgcaagcgg acgtgcaaca gtcagaagaa cgggtggatc
     8521 agcgaccatc aggaaaacta atccaacaca cattagtaac aaaagaaaat acggaagaaa
     8581 tccaaaaaaa atttgcagaa tacttgatgc tggaagctaa aaaaagaaga ataaaatcaa
     8641 tttttgccca gttttaatca aaaacagtaa tagaaggaga aatacaatga gttgtttagg
     8701 aaatgtcatc tggtttatat tcggcggact aatcggtggt cttagctggt tagcagcggg
     8761 gatactttgg tgtatcacga tcataggtat cccaatcggt atgcaatgtt ttaagatcgc
     8821 ctccctttct tttgcgcctt ttggcaaaga agtaatttat caccagtctg gcatgtcatt
     8881 gatcgttaat atcatctggc tgatcgtttc aggcctacca attgctctcg cacatgtcgt
     8941 cagcggcatc cttctatgta tcacgatcat agggatacct tttgccaagc aatctttcaa
     9001 acttgctcga ttggcgttga tgccattcgg tgcagaaatt gtataaaaag agaataataa
     9061 aggttagaaa acgcatagag tcaggtggga tacatgtatt tcacctgact ctatgtgttt
     9121 tcctgtataa gttctcccat gtgactttta cgtcttttat ttatatggga ttactgttta
     9181 tggaaagttg aaataatttt cattaaagaa ctctttctat atttttgaaa gcgtagtgga
     9241 gcttcgaata tctgggaagg acattttgca gggattgaaa aaagatattg tcctatcgaa
     9301 aaattaatga atttagacaa attgccagca acaggcttca aacttagttg ctatcctgtc
     9361 aagatcaaga aggcaagtgc gggttggata agagcagggg caatgatcga agagaaaaag
     9421 aaagaataag ccaaaacatc tgtatggaag aa
//
LOCUS       DARDHA010000107         9360 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000107 DARDHA010000000
VERSION     DARDHA010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.108"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(92..760)
                     /locus_tag="SDA10_002019"
     CDS             complement(92..760)
                     /locus_tag="SDA10_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG4240910.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            893..1180
                     /locus_tag="SDA10_002020"
     CDS             893..1180
                     /locus_tag="SDA10_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240911.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1596..3134
                     /locus_tag="SDA10_002021"
     CDS             1596..3134
                     /locus_tag="SDA10_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEG4240912.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3131..4096
                     /locus_tag="SDA10_002022"
     CDS             3131..4096
                     /locus_tag="SDA10_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4240913.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4083..5405
                     /locus_tag="SDA10_002023"
     CDS             4083..5405
                     /locus_tag="SDA10_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG4240914.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5468..5572
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5646..6389
                     /locus_tag="SDA10_002024"
     CDS             5646..6389
                     /locus_tag="SDA10_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4240915.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6404..7396
                     /locus_tag="SDA10_002025"
     CDS             6404..7396
                     /locus_tag="SDA10_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4240916.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7408..8145
                     /locus_tag="SDA10_002026"
     CDS             7408..8145
                     /locus_tag="SDA10_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4240917.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8135..8857
                     /locus_tag="SDA10_002027"
     CDS             8135..8857
                     /locus_tag="SDA10_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEG4240918.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8941..>9360)
                     /locus_tag="SDA10_002028"
     CDS             complement(8941..>9360)
                     /locus_tag="SDA10_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEG4240919.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3067 a         1607 c         1972 g         2714 t
ORIGIN      
        1 tttttttata aagaagattt tactgaataa tatcatttta gtttacataa tgatatttct
       61 gtaaattaaa taaagtagtg aaaaataaga atcagtgctc taactgttca agaggatcaa
      121 gtggttcaaa tgtttctgta gtctgttttg cttccaattg gggcaaaaca gatccaattt
      181 ccagatactt cactttattc aatgtataag atagaataga acagctaagt aatgtataca
      241 gaaaatgtgt gagatccaaa taaagcaagg agacaactaa aataccaaaa gtaatcaaga
      301 aaagactttt gccaatctta atatgaaaat atttttcaat ggcttgataa atgatatctg
      361 ttccgcctgt gcttccgccg aatcgaaaga cgagaccgca accaatgcca gaaagaatac
      421 ctgtcatcaa actattgatc agtagactat ctacagcaaa atgtatatga aggctctccc
      481 ataaatgtaa aaagaaaact agcgcaaatg ttccgacgat cgtccggaga aaagcgtgtt
      541 ttccaaaaag aaacaatgag ataagcaata aaggtaaatt aaaaagcagt gaagtgacag
      601 aaggagcgaa tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc ccaccatctg
      661 ccagcttaga tggaatattg attcctgtca cagctaatcc gtagattcct gcgcctaaaa
      721 tgattccgca caattctgta aataaattta attttttcat ttctagcctc ttttcttttg
      781 aaaattttca gcaacttctt tattatactg acttatactc ttacttcaag aggaaaatga
      841 aaaaaattat ttttggctgt ttctttttat ttatcaaata aggagtggaa aaatgcgagc
      901 agcagactta ttgacaattc ttacaaaatc acccaaaaaa acagagtatc aaggagtttt
      961 tttggctcaa gaacaaaaac tggtgcctgt gacaatggtc cagataaata aagcaaatca
     1021 atttatcttt ttatttgaac acggcaaagc accacttgct atgaaagaca tctttctttt
     1081 tttgatgaag aaccgtgaga aagctgttta ttatcagtca aaagaagaac tcattcctct
     1141 ttatggagtg aaagagatag aaaataaagt ggtgatataa gtaaaaataa gaaaacaaga
     1201 cgtttatgat gtcaaaacaa ccatttttta catttttggc aaaaaagtgt catgatacag
     1261 tataatattc gttgaacagg ccaataaagg tgttctcccc cacgagattt ctactttatt
     1321 ggctgtttct ttttattaaa ataaaagttg tgtgtgtaaa aaagatatag ctgattccaa
     1381 gtgacaattt cgtatagatt tttatggtat tcgtagtgaa aaaacgagaa ccaaaaagtt
     1441 gatccgactt tgttgcttgg attctttttt tgtcagcata tttagaggta tttttttctt
     1501 ttttttgttc gtgctgtaag gttaaggcaa gacaagttag ttgggaaaaa ggaaagaaat
     1561 gtttatactt aaagaataac agaaaggaag gtcgcatgaa taattttata caaacattaa
     1621 gtgaaagaaa aggcgagttg ctggaagctg catttcaaca tctgtccatt tctctcattt
     1681 ctttgctgat tgcagcactg attgctattc ctttagcgat ttgggcagcc aatcataaaa
     1741 aaatggcaga agtcttattg cagatcacgg gtgtgctcca aacgattcct tcacttgcct
     1801 tattaggttt acttatccca tttgtaggga tcggaactgt tccagcattg attgcacttg
     1861 ttatttacgc tttgcttccg attttccaaa acacgtatat tggtctagct gaaattgacc
     1921 cgtctattga agaagcagcg gtagcatttg gtatgtccag aatgagacgc ttgctgaaag
     1981 tagagcttcc catcgcatta cctgtcatta tctcaggtat ccgaactgcc ttagttttaa
     2041 tcatcggtac ggctactttg gcagcattga tcggagcagg tggattaggg acatttattt
     2101 tgttaggtat tgaccgaaat acacctgttt tgacattgat tggtgcgatc agttctgcct
     2161 tgcttgcaat tatattcagc ggactgattc gctggttgca gcataagaaa cctcgatatt
     2221 ccatcattac tttagcagca gtggtgtgtt tatttggcgg cgtttcttta tatcagtctg
     2281 ccttgtttaa aaatgaaacc atcacgattg ctggaaaatt aggagcggaa cctgaaatcc
     2341 tgatccaaat gtataaagaa ttgattgaag acaacacaaa aacagaagtt gaattaaaac
     2401 cgaattttgg caagacgagt tttttgttca atgcactgga tcaaggtcaa atcgatatct
     2461 atcctgagtt cacaggtact gtcctagaaa gcttggtgaa ggtacctgaa gcaatgaaaa
     2521 gtgctgattt gacaaaagaa aaaacatatg acgaagcaga agaattattg aataagcaat
     2581 tcgatatgac attattaaaa ccaatggctt accaaaatac gtatgccttg gcagtcaaga
     2641 gatcttttgc gaacgaacat caacttaaaa agatttccga tttggcagct atctctgatc
     2701 agatcaaagc gggattcaca ctggaattta ttgatcgttc agatggatat aaaggaatcc
     2761 aagaagcata tggcttgaat ttttcaagtg tacaaagcat ggagccaagt ttgcgttacc
     2821 aagcaatcaa taatggagat gtcaatattg tggatgccta ttccacagat agccagctca
     2881 aggagtacga tctagtgact ctagaagatg atcgagggtt attcccagct tatcaaggcg
     2941 caccgctgat gaaaaaagat tttgctaaag aacatcctga aatcgttaaa gcactgaata
     3001 aattaagcgg aaaaataacg gaggatcaaa tgattgatat gaattaccaa gtcaatgtcg
     3061 aaaagaaaaa gccgacagaa gtagcacgaa tcttcctgaa aaatgaagga ttagtaaagg
     3121 aggaagatca gtgactaaat taatcgagtt caaacatgtg caaaaaaagt atgatggaaa
     3181 gtatgtcatc gatgacctaa atctggcaat caaaaaagga gaaatctttg ttttggttgg
     3241 tccttccggt agtggaaaaa caactacttt gaaaatgatc aatggattat ctaaaccatc
     3301 agcaggagac atctacttca aaggaaaatc gttaaatgaa tataatctgc agaaaatgcg
     3361 gtggaatatg ggatatgtgc tgcagcaaat cgctttgttc ccgaccatga cagtcaagca
     3421 aaatattgaa gtcatccctg aaatgttagg ttgggaaaag cagaaaagag cagaccgtgt
     3481 agatgaactt cttcaaaaag ttggcttatc tcctgatatt tatcgagatc gtatgcctag
     3541 agagttgtct ggaggagaac agcaacggat cgggatcatc cgagctattg ctgcaagccc
     3601 agatgtgatt ttgatggacg agccatttag cgcattagat ccgatttcaa gaaactcatt
     3661 acaagaattg gttttgtcct tacacgaaga attgggcacg accatcgttt tcgttacgca
     3721 caatatggaa gaagcaatca agctaggtga tcgtattgca tttatgaagg atggagaaat
     3781 tatccaatgt gacacaccag agcagttatt gatgaatcct aaaaacgact acgttcggca
     3841 tttctttgat gaaccgaaac agacgaaaga gtggcgagta gaagatttag tcgtcaatgg
     3901 atatttctta aatgaaatac cagaaaatgc tcgtcaacaa gtttgctttg atacgcctat
     3961 gaaagaagta tattcgcttc tttcggtgta tccaagcatt gtgatagtag aaaagcaaag
     4021 aagtattggg tcgctgacaa gtaaagaaat ctttgctttt ttaagtcgag aggaggaagg
     4081 aaatgaaaac atatgatgca atcatcatcg gttcaggtgt gagcggctta agtgctgctt
     4141 atggattgaa agaagcaggt aaaacagtac tagtggtcga agaagatcta tggggcggga
     4201 cttgtcctaa ccgtggatgt gatcctaaaa aagtcttgtt aagtgcagta gaggcacgta
     4261 atcgggtaaa acaattgagt ggaaaaggtt ttaacgagat cccaactgcc aactgggaag
     4321 aacttcaaaa attcaaacga acatttacgg atccagtccc agaaagccga aaaaaacaat
     4381 tagcagaagc tgaaatcgat catctttcag gaaccgctcg ttttttggat gattcatcta
     4441 ttgaagtcaa cgaagaagtc ttccatgcgg attacttagt tttggcaacc gggcaaagac
     4501 ctacgatctt accagtggag ggaaaggaat acttgaaaac tagtgcagac tttttatcat
     4561 tgcctgtact gcctaaagaa atcattttta tcggtggtgg ttatattgct tttgagctag
     4621 ctacgattgc caatgctgca gggagcaaag taaccattgt ccatcataat caacggccat
     4681 taaaagaatt tgaggcgtct ttggtagagg aagccgtcca tcaaatggaa gcatctggca
     4741 ttcagtttgc ttttggtgtt gaaacccaaa agatcatttc agaaggtact cgctatcgtt
     4801 tagtcggaaa agagacggaa ttagtagcag atatgatttt ttgtgcgact gggagacagc
     4861 cgaatacaga atctttagca cttgagcaag cgaatatcgt ctttgataaa cacggaattg
     4921 cagtcaatga ttatctgcaa acgagcaatc caaaaatttt tgcgtgtgga gatattgttt
     4981 caaggaaaac cccaaaattg acaccagtcg ccacttttga aggaaattat gtagctaagc
     5041 ggataacgga tgcaacaagc gaacctatca aatatccgat tatacctacg atcgtctacg
     5101 ccagtcctaa acttgctgaa gtaggagtta caaaaagtca cgcctcttcg tcagatcaag
     5161 tggtagagat ggatcttacc agttggttca cctatcatcg tgtcaatgaa ccagttgcta
     5221 aggctgaatt gacctttgac cagcaaaatt atttgattgg tgctgcagtg attagcgagc
     5281 aagcagacga attgatcgat gatttgacac tcgtcatcaa tcaaaaattg actaaaaaag
     5341 agttggatag ctatatcatg gggtatccga cattggcaag tgatctctcc tatttattga
     5401 aataatcagc atgataacta aagcgcttgt tgctttttcc ttcttgtgct acactttaaa
     5461 aaatgaatac ctgtttctgg ggatgtcgta gacgacatga gagtaagttg tgaacttaga
     5521 cccattgaac ctgtcagtta atactggtga agggagaaac atagcagacg cttgaagcgg
     5581 gttactatgt cgtgcccatt tcaagcgttt tttgttattc atattagaag aaaggaggta
     5641 acaaaatgaa gaaaaatctt tatccagcag tcagtatggc attcatttta attatttggc
     5701 agattgttgt catagtttgg aaaatacctg cctttatttt acccggacct gctgctgtat
     5761 tttctgcatt agcggcagat gcacaagtat tactttccca ttctgtcacg acattaaatg
     5821 aagcagtgct tggtttggta atagctagcg ttctttcttt tattactgcc ttggcaatgg
     5881 attattggaa gtttttagaa atgtcttttt atccgttact agtgatttca caaatgttgc
     5941 caatcatggt gttagggcca ttacttaccc tatggtttgg tttcggtatg cttccaaaag
     6001 tgatccttgt tgtcttgata agctattttc cgatcgtagt ttctttttcg gatacattga
     6061 ggaaagtatc aaaagagcaa atcatctttt tgaaaacgat gggggcagat acagggaaga
     6121 tttatcgaat atataaaata ccagcgggtt tatccggctt cttttccggt ttaaaagtgg
     6181 ctgctactta ttgtgtcagc ggagcagtcg ttggagaatg gctaagcgct caagcaggct
     6241 tagggtatta catgatacgc gtaaaaaaca gctaccagat cgataaagtt tttgctgcga
     6301 tcatttgtgt cattctattg agtttattgc tgaatggcgc ttgttcatta ttgaaaaaag
     6361 ggtattatcg tatgctttat attttttaag agaaggggat caaatgaaaa aattatggct
     6421 tttattaccg ctactgttgc ttttatccgc ttgtggcact gcaaaagaaa caagtagcaa
     6481 acaagaaata aaggatttga aaaaagtgac acttgttttg gattatgtac cgaatactaa
     6541 tcatactggc atctacctag caaaagaaaa aggctattat aaagaggctg gtttaaatgt
     6601 ccaaatcatc gaaccaggag ataattcgac aagtattggt ctagtaggtg cggacaaagc
     6661 acaatttggt gtaagttatc aagaagatgt cacttatgct cacgcagatg gacagaatat
     6721 accagtcaaa gcaattgcta cagtgatcaa acataatact tctggattcg ccaccctatc
     6781 agacagtaac atccattcac caaaagattt tgaaggaaaa acatatgcag gttggcaaag
     6841 tccaagtgaa gaagctgtct taaaagctgt gatggaaaaa gacggcgggg acttttcaaa
     6901 actgacaatg gtaggaagca atggagaagg gccagaaagc ttaggaaaat cctcagatat
     6961 ccaatggtac tttgaaggtt gggatatgat caaagcaaaa gaagcgggta ttgaactaaa
     7021 ctatatccca ttgaaagaat tagatgaacg gttagattac tatactccgg taattattac
     7081 gaatgatcaa ctgatcaaaa gtgatccaga attggtacag tcttttatgg atgccacgaa
     7141 aaaaggctat caggaagcta ttaaggatcc aaatgacagt gctaaactgt tgcaaaaata
     7201 tgcaaaagaa aatgatcgga cgtttttaga agaatcacaa gcattcttgt caaaaaatta
     7261 tacggatgat cctgagaatt ggggattgat ggaagaaaaa gtttggcgca attatacagc
     7321 ttttatgcag gaaaacggat tgatcaagca agatgtacct tcgcaagaac tattcactaa
     7381 tcagtttata aaataggagt gatagatatg tcaatccaaa tcagccactt gaatgtagtc
     7441 attgaaaacc tgaaaatatt agaagacatc catttcacta tccctaaaga tacctttaca
     7501 tcgattgtcg cgccaagcgg agccggtaag tcaacattgc tgaaaacttt aacaggagtc
     7561 cagcctatga cttctggaac gatcaaagta gacgatcaaa aagtaactgg tctccataca
     7621 gcatttagtt atatgccgca agaagatatg ttacttcctt ggctgagcgt ctatcaaaac
     7681 gttacgttat atcaaaaaat caatcatcta acaatcaatg aagaacaagt aagggattat
     7741 ctggatatct ttgggttaaa cggctatgaa cattttttgc cggaacaatt atctggcggg
     7801 atgaagcagc gaacggcctt gttacgaaca atcatgaatc cttcttctta cttgcttctt
     7861 gatgaaccat ttggagcgtt agatgctatg accagagggc agatgcaaga ctggatgttg
     7921 aagctgccta aaaaagcaaa gcggactact ttacttgtga cgcatgatat cgaagaagca
     7981 atttatttat cagacaggat tcttgtgctt tccgcccgtc cggcacatgt gattgctgag
     8041 attcaagtgc ctgaaaaacg acggtcacgt gaatggctac tgcagcaatc agagttgaag
     8101 caaacgattt atcagttatt ggcaggtgaa ttagatgtta agtgatgctc attgtcattt
     8161 agatgggagt cagagtttag cactccttca gcaggaacac acgattttga cgatcatcaa
     8221 ttgtgattcg ccagaagaat ggaaggaaaa cagacagctt gcagcaagta agactcaagc
     8281 cttaagttac ggtatccatc cttggaaagc agatagctat acgtttgaac aagttgagcc
     8341 ttttttgaaa aaagcaagga tcatcggtga aatagggtta gataatattt ggacgaatgt
     8401 accgatgact acacaaaaaa aagtatttga gcgacagctt gcttttgcag ccataaatga
     8461 gaaacctgtt gtactgcaca caaaaggatg cgaaaaagag atcttaaatt atatccaagc
     8521 ttaccctaat cactatttga tccattggta ttcttctttg gactatcaac aagactatat
     8581 tgatttaggc tgttatttca gcattggtct tgatttgaaa aagaatccag cagtctggca
     8641 actagctaaa gcagtcccaa tcgaccactt acttattgaa acggatggac tacatgcagc
     8701 agagtgggct ttagggggga ttttttcaga aaaagattat gtctcactgc ttgaagaaca
     8761 gttaaagaca atcgctgaga tcaaagagct atcagttgaa gaagttaaaa aaaatactta
     8821 tcaaaacttg gaacgtttca ttagattagg taaataaaaa agagatgtga cacaactagt
     8881 gtcgcacctc tttttggctt tctttgtata aaagtaaact gtttgattaa taaatgaatc
     8941 ttagtaccaa ccgtttgctt cccagaatgc tttcgccgct tcccaagaac cataacgtga
     9001 agtaacatat tgttctgcta ctctttcttg gtttgcagca gagtagtcac catttaagta
     9061 agaagaatct aattggtaac gtccgatgta acgtccgttc gttgctgtgt aagaaccgct
     9121 tgattctttt tgtgcgatcc attcttttgc agagcttgtg cttgctggag tagcagcagt
     9181 ttctgttaca gcaggagcag gttgtgtttc agcaacaggc gctgcttgtt tttccgtttg
     9241 aaccgtttct tgtacaacag gttgttcagc ttggactggt gcttcttgaa cggctgcttg
     9301 aggttcagca gctgcttgtg cagagccttc tgttgggatt gttaattgtt gtcctgagta
//
LOCUS       DARDHA010000108         9194 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000108 DARDHA010000000
VERSION     DARDHA010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.109"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            278..787
                     /locus_tag="SDA10_002029"
     CDS             278..787
                     /locus_tag="SDA10_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG4240920.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="SDA10_002030"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="SDA10_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG4240921.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="SDA10_002031"
     CDS             2322..3302
                     /locus_tag="SDA10_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4240922.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="SDA10_002032"
     CDS             3424..4842
                     /locus_tag="SDA10_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEG4240923.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="SDA10_002033"
     CDS             4913..6382
                     /locus_tag="SDA10_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4240924.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="SDA10_002034"
     CDS             6392..6859
                     /locus_tag="SDA10_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4240925.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="SDA10_002035"
     CDS             6895..7767
                     /locus_tag="SDA10_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG4240926.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..>9194
                     /locus_tag="SDA10_002036"
     CDS             7848..>9194
                     /locus_tag="SDA10_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG4240927.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKF"
BASE COUNT         3169 a         1355 c         1840 g         2830 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa attt
//
LOCUS       DARDHA010000109         9070 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000109 DARDHA010000000
VERSION     DARDHA010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.110"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1872
                     /locus_tag="SDA10_002037"
     CDS             <1..1872
                     /locus_tag="SDA10_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG4240928.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
     gene            complement(3807..4472)
                     /locus_tag="SDA10_002038"
     CDS             complement(3807..4472)
                     /locus_tag="SDA10_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4240929.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            4652..6634
                     /locus_tag="SDA10_002039"
     CDS             4652..6634
                     /locus_tag="SDA10_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEG4240930.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            6638..7276
                     /locus_tag="SDA10_002040"
     CDS             6638..7276
                     /locus_tag="SDA10_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4240931.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            7304..7732
                     /locus_tag="SDA10_002041"
     CDS             7304..7732
                     /locus_tag="SDA10_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240932.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            8189..>9070
                     /locus_tag="SDA10_002042"
     CDS             8189..>9070
                     /locus_tag="SDA10_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEG4240933.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFT"
BASE COUNT         3149 a         1389 c         1713 g         2819 t
ORIGIN      
        1 agtgctgaaa atatggaaga aatcaaaaaa atgtttccga aatccacttg gtttggcttt
       61 acaggtacac cgatttttcc tgagaatcgg aaacaagcca aagggcaatt ggcacgtaca
      121 acccatgatc agtatggaga ggtcttgcat acctatacca ttaagaatgc gctagaagat
      181 ggctctgtct taggatttca agtagagcat gaaaacaccg tggagccgac ttcactagag
      241 aataaaatct atcggaaatt aaaagaagta gaaacatatg cagagtattc atcagaacag
      301 atcaatcgta tgattgatca aatggaacct gtcaaaaaag aaagttattt ggacccagct
      361 gtttttgaag cagatgaaca tatccaaaaa gtcattcata aaatgttccg tccagataat
      421 gcctatacaa aatttgattt tcgaaatggc aggccaacga aatctgcaat cttaaccacg
      481 agttccattg acatggccaa aaagtattat cgggccatca aagagatgac gaaagagcct
      541 gattggttaa caaaagagtt tcccaatcag ctcattcgtg aaggacgaac gatggaggat
      601 cctgattttc cacgaatcgc tattacctat tcattagaag aaaatagccg agattcaagt
      661 gcccaacaag aagaaatgca aaaaattatt gaggaatata atcagtatta tgatacttcc
      721 tggtcattag cagacattga gcgttataac ggagacatca ataatcgttt ggcacgaaaa
      781 agagcggaat tcaaacaatt tgggaaacaa atcgatttag cgattgtagt ggatcgtcta
      841 ttaacaggtt ttgacgcccc tacgattcaa accctatttg tggatcgtaa tctagagtat
      901 gcaggattga tccaagcttt ttcacgtacg aatcgtacgt atcctgataa gacaaaagga
      961 ttgattgtga cgtttcgtaa gccagcgaca atggagaaga atgtcgagga tgcgacaaaa
     1021 ctgtattcaa aagcaaaaga agaatcaggt ttgatttatc caagttatca ggaatccaaa
     1081 aaaagattca aacaagcata tggtaagtta aaagaggttt catccatgga atccattgat
     1141 gaacatacgc cacttgaaat ccgtatcgag tacgtcaaag catttcaaga attgaatcgt
     1201 gcttatgaat cgttagtgac ttatgacgat tacaatgatg acatggtgaa atctccaact
     1261 ttgaacaatc aaattcaact tttggaagaa caaattggcg tgtacgagac cgtgaagggt
     1321 tcattaatag aggaagaacc agaaaaagaa ggagaagatt tttcaacgat cgaattttat
     1381 agtgaaaata ccactaagtt gtatgatatt gattcaactt atattgatca attactagga
     1441 acttatgctg cgaatagttc agatgcacga gaagaaattg aaaaagcttt ggctaaattg
     1501 aataaaacag aaggagtcaa agaagtttac cgacaaattt taaatgcatt tgatgtgggg
     1561 actttagata gtaatgaaga tatctttgtc attaaacgtc gctattttac acaagcgagt
     1621 gatgatttga ttcatgtctt ttccaatgag tggtttgttt ctgagaaaga attacatgct
     1681 tctaatctgc aatatctacc aggtgaagat ccaattccta atatgaaagc aattatcaac
     1741 agtaaggatt acgaaggata taaagccaaa catccagaag cgaagccatt taaataccca
     1801 caagaaatga agcgagcatg gcgtaaaatg ttagatgatg agttgattcc acttgagaat
     1861 gaattgaggt aaacgagaag agttaagaaa attgtacgat tcaaagatgg tagtcaagtg
     1921 gaagtgtaga aaatagggat agatgtcttt tatttgttca cattttataa cgaagtattt
     1981 aagacagcta ttgaataatg tttcctccac ctttttcatt catttacccc accccgttgt
     2041 tatatgaccg tgttttgaga ggaaccaata aaaagcatag gctaactagg ggttcctttt
     2101 tcttttgttt ttttggaatc cgcttaagtg aaatgtatct gaaatgaaac ggagataaag
     2161 tgattttttg ctataatgac aaggttttag ctgtttcaga taccatgtaa cccaaaatag
     2221 tttattgaga aaataggaga gtatttgata ctaactagta ggaaaaaatg agataaaaat
     2281 taaatggaat catttcaggt aatctcaaat aaaaaaatat acagtcctgt ggcactttac
     2341 ctatgggact gtatattttt tactctcagc gggcttgttt tgactccgtg tctgactccg
     2401 tcgtattgat tttaaatgat attcgatgac catttaacag gtcatttttg aaatttttgt
     2461 ctctatatga ctcagtttga taattattga tagatcaatt tgggtctata tcgaacaagg
     2521 gaataatcca ataaaaaatg atttagaata tcaaaataac aaaaaagagg actacctgta
     2581 ttcgtcctct tttttgacca gattttgacc agtctaattt acattctaat atattcatat
     2641 gagctaattt tttgattaaa tggtaagttt ttccttgttt gttttagttt aaactagtgt
     2701 gtaatttaaa aatctatatg gaagggtctg tggggtaata cttaaataaa ggctaggaac
     2761 ctgataacat caaggttcct agccttttta taataaaatg aagcgaaaat aatttctttt
     2821 aaagagccga gattatcagc tatataagag tgaatttgat gcaaaaaaca gagccgaaac
     2881 agtcttggtg ttcgtagctc tgcttatatg tttcgaattt aagacaaaga gttggcaaaa
     2941 gatgttgata aggaagataa gataactgtg ttatataaac taggtaacta actgtaacaa
     3001 gctaatttta agagattctt attagaagac gtttaggaat ataactaaga aaatgattat
     3061 acaggtaatt gtcatttcaa taatgttttc taattttcgc gtctttgttt taagaaaaag
     3121 gccagaatca gttggaaaaa agttcgtacg atagtgaaga ggggcaaaat ttcctactaa
     3181 tattcccact aacaagagat aaaacggcca ggttggtgtg attcctctat aaaaaaatgc
     3241 ccatgctacc gaaatcccat agattaacaa ctgaacaatt tttacaatga aaaaattgtt
     3301 ctgccattac tcaaagtctc ttcctccttg tatcgtttat ttgtttcatt ttatcattat
     3361 gataggcgct tgaacaatcc attttgtatt ttattgagta ctcttggata aactaccagc
     3421 gattatcata tttatttatg ttactgtaag ccatcatata aaagtaaatg agcagatcat
     3481 agaaatataa aaaagaacta aaattgatct aaattatgtg cagcttgact acgtaccaat
     3541 ctatattact tactagattg gtcgttaatc tattcggatc gaatgagcaa aatatttttg
     3601 tgaatgtcca tccttgatta tgattccaaa aagaatcatt gggaggctgt ggcgtaaata
     3661 ttatactata agccagaaat acctatcatt ctgaactttt ttatttagga actttcatct
     3721 caaggctttt tcaattaatt tgtacccctg ttttttggat aagatcatgt gcattgattt
     3781 tttctatctc tacgttcatc tcctacttaa tgcctatctg gctcctgagg gcttcttttt
     3841 aaggcgtcgt catagtcttg gctgaggatt tcttggtaat ctgagggaat ggcttcccaa
     3901 gtgagcaggc gaacgttcat gacgtgtttt cctttgtgtt tgattacagc atggtttttc
     3961 tctttttcta cctctccagc gtgaaaagac agccatgtac catggccaaa gatatcatta
     4021 catacccaac gttcgatgga attgtccacg tatcctacag ggagtgtggt atagccgatt
     4081 tgtggtttca aaaaaggatt gaatcgatcg atcgtttcat ttttgtattg ataattgata
     4141 agaaaaagat agaccaaaca aaagacgagc agaaagaaag atagtgtctt tttgagtgag
     4201 aagggttgtt ttgatgaata agatttcttg ttaacaagag gttcatctgg caatgttggt
     4261 tctattgatt cggtagctga ttcaatagaa ggaaaatcaa tcggcagacc taaagtatga
     4321 tgtaaaagtg acagcatttc aaggtctggc attgtacgag aattttccca actagaaatc
     4381 gttttatcgg aaacaccaat tttttcgcct aattcttttt gcgtaagctt acaatttctg
     4441 cggtaagttc gtattttttc tccggtgttc atcttcttct cctttcgatt ctacagtata
     4501 tagatttatt ctacatgacc tatacacttg atagatacgt ttttttgatg aaaaacggta
     4561 tgattttatc aaagaatcat ttaagtaagg gagagtggtg ataatcaaaa aaaagttcac
     4621 agttttactc acaatcatag tgaccggtat catgagtggt tttgccttgg ttagtataaa
     4681 taaacaggta ctctatcgga caaatgaatt tttccatcaa acagatactt atactccgat
     4741 tcgattgcca caacatttta ttgggcaaga agaatatgag cagctaatca tggtatttga
     4801 aaatgtctca tcagaaactg agctttctta tatgaaaaaa acagtaaatg aatccaatga
     4861 agtagataca tcttggaact tcaagataca gccaaagctt gaaatgacat tgtattatca
     4921 aggggatgtc aaatcttctc acgagaaggt accctattct aatacctcct ttcgcgtaac
     4981 gtccattgaa gaatcgatca aaataaaaga ttttgaaggt gaatttttct tgatgacgag
     5041 agaaaaagaa gcctatgaac ggttcatttc actattcact gatcgattca atcaagcatt
     5101 tcacactgat tatgccacta atatttttga agactttgaa aatagctatt cgtattcggt
     5161 tatttcaaat acagacgtat ataattatcg agtgtattta actattggat tgattctttt
     5221 attccttttg ttatcatcct ttttcttatt gatgaataaa gaaatcagtt tattgttgaa
     5281 taatgacttt tcattacatc aagtattgtg gtgtttattg gggaaaaaga aaatcattat
     5341 tacattcata attgcaggaa tcattggtgg ttttattttt atgtattata aggagcaatt
     5401 tctttatttg ttccagatgt acgttttctt gttcagcgga atatatggta tctctttgat
     5461 ttctctttta ttagttgaaa aaatcgcttc gttaagagaa gcgttcaact atatccaata
     5521 cattcttttt acatttggat tgttgttttc tcttcttttg accgttgcca atgtcgaatt
     5581 agcaactatc gttttttctt cttcacagtt agctgtttcc catgaaccac caaaagaaat
     5641 cgctggtgag gactatcatg tattttatcc agtaaccatc gggaaaaacc acgtggattt
     5701 tatttattcc aatcgttttg catcagcagc agatcaggaa ttatatgaaa ctttgaataa
     5761 aaacggaagt atattagtca atgtatcaga ttacttgaat gaagaaaatg aacagtttgg
     5821 cagagggata aaaataaatc tgaattatct taagaaattt cctttgattg atgtctctgg
     5881 aaggttgatc caaataaccg aaaaagaaac gcaccctgtt atattgatcc ctgaacgata
     5941 tagacataca gatttaaaaa atgacctggc gcaaataaca gaatattatc aagaaatcag
     6001 tgaaaaagaa ccgaaatttc tatttataaa aaacaatcaa cccatttata catttattcc
     6061 ttctatcccc tggatcgaac attatccggt tgttgaaata ctgacattga aaaacagtat
     6121 gtatttggaa agaaatattc tcagcggaga aatctatcca ccgttaaaga tcaagatagg
     6181 ctctttgtcg aaagaaaggt tatttgagct gattgaagaa agtcagttac gtgataactt
     6241 gcagttatca tttccgtata ctcagactga agagatagct gtcaaagtgt taagcagatc
     6301 atttcattac ttgatacaga tatttttact cactttcttt tctttcttct tgttgagcta
     6361 tgtcatgtta tgcatatatt ttgtctacaa ttgtcggaaa atcgctatct ttcgttcaag
     6421 tggttattcg ctctttgaaa cgtataaaga tttcttcatg atgaacctca tcaaatgggg
     6481 aacgacaagt gtaatcttcc tttttcttat agaaagagag cctaaatatc tttttaatat
     6541 ttttttcttt agcttcattg attttattct ttcagttgtt tttattttgt ctacagaaaa
     6601 gaaaagtcag ttattgctga tgaatggagg ttaacaattg gagccgttga tagtgataaa
     6661 aggtgcgaaa aaaatcattc gaaacaaaac gattcttcat ttggatcgtt tcactgcgga
     6721 tgctggggat cttgtgactg ttgttggaga aagtggagca ggtaaaagta cgttattgaa
     6781 tatattagga ttgaatgatc agttcagttc aggacaattc tatttgttcg atcaagagac
     6841 gaaacaatta actgggagag agaagcgctt gataaaaaga aataccatat cttttctttt
     6901 tcaagatttt gggttgattg aatatgaaac aatcgatttt aatttagata ttggattgaa
     6961 gttttcgaat ttaaatgttt cagataagcg aacagcaaag tatgaggcat tgaaaaaagt
     7021 aaatataaac aagaagctaa gtacgcctat ctacacatta tctggtggtg agaagcaaag
     7081 aatcgcctgt gcgagagtct tattgaagaa tagcaaaatc attttagccg atgaaccgac
     7141 tgggtcattg gataaaagaa ataaggataa agtagttgaa atcttatcgc aacaagcttc
     7201 ccaaggtacg ttagttatcg tagtcacgca tgatccatat ctgaccaaaa taggaaacaa
     7261 gaagatcatg ctttaaaaat caaaaaggaa aggatgtcgt tttatgaaaa aagcagttat
     7321 tatttgtgca ctagggatag gttttgttgg aggaaacgct atgtcatctg aagtggaagc
     7381 aaattcagtt cacggtgaaa tctattacaa atctttaaag ccaggagaga ttcattttga
     7441 tgctaaaggt aatattatta agtcaaattt accagtagac cgggaaattt ctagaacagc
     7501 aattaatcat agctctggaa gatgggttta ttatagtgat acaacttgta cgagaaaaac
     7561 aggtcattct aaccactata gtcgtgtcta taatcgtcac ggtgcaaaag cgaaagttgg
     7621 aaactctatt cgatcagcaa atgcaacaaa aggagtatgg gcctatgcaa gtgcgacagg
     7681 aaatagaact gacacattct catgttggta caatccaacc ggttggtatt aagtatccaa
     7741 aagtttatat aaaatagaaa aaacatataa tgaaacagcg ttaaaagtta tttttggtag
     7801 ttttaatata ttcattttta gttgtataaa aatatatcat aataaaaaca acaaaaaaat
     7861 gtctggaaaa tcgaacataa aagtgtctga tttgaatatc ccagcagcgg aagaagaaaa
     7921 acaaaatcga taacatttgt tctaagaatt agttacgtta aatacggaac gcaagataga
     7981 cttgaaaaac ggtaacaaaa gaagtagtat attaaggtgt gatgtgctac ttgacattta
     8041 ttctgctgag cgaactttct ggattgtgta gccttatatc tattcaggac gtatcgagca
     8101 gattagtaaa ggtgttcttg atgattttca tcttgaacac ctttgttttt agcaattcgc
     8161 aaaaaacgaa aagtaggaga attgataaat gaaaaaaaga tttttagcac ttgccatcgt
     8221 actaggcact ggcttattgt caggctgtac gaatgccggt gaaaaaacag ccgttagtta
     8281 taaaggcggt acaatcagcg aacaagaagt aatggatagc ttgaaaaaaa tgcaaggagc
     8341 agattccgct gtgcaacagt tgatcgttta tcaagtattt gaagataaat atggagatga
     8401 cgtatcaacc aaagaaatcg attctcaata tgatcaaaca aagaaacaat tgggtgattc
     8461 atttgattca caattgaaat ctgcaggtta cacagaacaa acattcaaag acagtatcaa
     8521 acaaagtcta gcctttcaag aaggtctaaa gaaacatatc aaattgaccg atgaagattt
     8581 aaaaacggct tgggaatctt tccatccaga agtggaagct caaattatcc aagtagcaag
     8641 tgaagatgat gcaaaagatg tgaagaaagc tgctgataag ggagacgatt tctctaaatt
     8701 agccaaagac aagtcaactg atactacgac aaaagaagat ggcggaaaag tgaaatttga
     8761 ttccactact acaacggttc cggcagaagt caaagaagca gcctttaagt tgaaagatgg
     8821 tcaagtttct gatgtgatca cttctaccaa tgcttcaact tatactacgg aatattatgt
     8881 ggttaaaatg gtgaaaaacc aaaacaaagg caacgacatg gataaataca aaaaagaatt
     8941 gaaagaaatt gcaacagaca caaaattaag tgacagtacc ttccagaata aagtaattgg
     9001 cgaagtattg aaagacgcta atgtaaaaat caaagacaaa gactttgaaa atgttttgag
     9061 tacattcact
//
LOCUS       DARDHA010000110         9063 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000110 DARDHA010000000
VERSION     DARDHA010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.111"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(89..2263)
                     /locus_tag="SDA10_002043"
     CDS             complement(89..2263)
                     /locus_tag="SDA10_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HEG4240934.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2279..4621)
                     /locus_tag="SDA10_002044"
     CDS             complement(2279..4621)
                     /locus_tag="SDA10_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240935.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(4643..7186)
                     /locus_tag="SDA10_002045"
     CDS             complement(4643..7186)
                     /locus_tag="SDA10_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG4240936.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7196..7594)
                     /locus_tag="SDA10_002046"
     CDS             complement(7196..7594)
                     /locus_tag="SDA10_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG4240937.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(7675..7959)
                     /locus_tag="SDA10_002047"
     CDS             complement(7675..7959)
                     /locus_tag="SDA10_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240938.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(7983..8981)
                     /locus_tag="SDA10_002048"
     CDS             complement(7983..8981)
                     /locus_tag="SDA10_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG4240939.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2578 a         1959 c         1423 g         3103 t
ORIGIN      
        1 ttttccattt gattcttcgt tagtttttat cagtttaaaa caaccaaata agaggaaact
       61 aacaataagt atcctaacaa taactttctt atttcgcata ttgtttctcc aaatttggtt
      121 tccgaatata gacgactcgc ggtggttcct ggacgtcttc agctgttcga tacaccacaa
      181 attcgcctgt tctcaatgct cgaatgcgat tcggattgaa tttaaagcgg tctacgtctc
      241 gtttcgtgcc tctttctcct gtccaatcaa cctttcccat atcagaataa cctgcttctt
      301 gtgtcgtcat ttccgttaat tcaatgtctt cttctgtccc cattaaatct gcccaataat
      361 caatttctgt cgggtcattt gtcttcccaa taaagaacgt attcacattc ccaatcaact
      421 gttttcgaag gacaggatca attctatcaa tgtccgcaag cgtttgaggg gcatacgccg
      481 catgcacccc agcgcctcgt cccttattga ctaagtctat aaggttatca tttagataag
      541 aagctggttc gtcaaatatt actaggactt tcgagcgatt ggctgtttct cctgcttgtt
      601 tatacacttc tgacgcaaag aaattaatat ctcccacaag catttgagcc aatgtccgaa
      661 taaactcact ataaatcaat ccgtttaacg agatataaac aatctttcct tctttaacaa
      721 cttcttgaat atctaattct ctttttcctc cagaagtatc aaagagttca ccaagctcac
      781 tatccaataa catattgata ttggtctgta aattctcaga agtcgcatac aaataaaacg
      841 ggttcttctt atgttcgtaa cgaacaaaca accgtttaaa caacgtttgc tcgatttctg
      901 tcatgagaga aatgttttct tttaataata gataatacgt ttctaagtca acggttttac
      961 gatcatgttc aacgattcgc tcttcactta actgttccac atcggcaaaa tcacgttctg
     1021 ttttgtccat cgcttcattc tttttcgttt ttttcttcgt ggttttcttc ttttctacga
     1081 ccactttttc tttaaataaa tctggatttt tcaactgaat acgatccgca aacaaatgta
     1141 agacgtttcg tggcaataaa taatgttgta accaaactag atttctttca attttatggc
     1201 tcttttggaa ctcgtctaaa agctgaatcg ttacctgtaa taacgctttg gctgctgtcg
     1261 aataatacac gctttctgtt tctgctagat tggttaattt atctcgaatg gcaatatcat
     1321 tgccattttt tacaggatta taacgcaaat cgccaccgtc cacaaagact tcaatcgggc
     1381 gattgtatgc ctctgccact tcttttaacg cttgtaaagt ctctcctgct cctttcccat
     1441 cgactaagat aacggggata cgaaacttac acgcatgttc tgctagtaat tggatcaccg
     1501 tcgtttttcc tgaacctgta gtcgctggaa taatcacatg ttggttcaat tcactaaagt
     1561 tcatcacata agggagccca tactcatctg ttcctaatag aatcactttg gctaatgaag
     1621 ccagttgttt attccgcaaa gcaacgtctt ttatgacaat tgctacctgg tacgcttgct
     1681 gatactctgc tgtatatttt tgggactcct gaaatttatt cttccgacgt tccttgtatt
     1741 ctttactatt tagcaaagct gtttgccgtt ctttctttga aacaactttt cttttttcaa
     1801 taaaagacgc atacgcaatc aaacaacttc ctgcacctgt cccacctaaa aggagggcaa
     1861 aaatcgtttg tccattaaac tgcaacgcat gagaaaacaa gtttaatgga aataataagt
     1921 aacacgacaa ataccctcta ggaagaacaa aaataaaaca aaatagattt aatagcgtaa
     1981 aacctaacgc aaaaataaaa aaaagagagc ttcttcgttt taagaagctc tcatactcaa
     2041 agtcataaat atgtgcacgt gtctcttttt tatctatcat aaagacaatc gctaaaggaa
     2101 tactgcctac aataaaaaat ggatatagac aaacacctaa taaagtgacg ataaaataaa
     2161 gttgttgaaa ccgacttttt ggcgtttctt tttcttgttt tcttttgcga tcgacagaaa
     2221 acatttgctc aaaatgtgtc ggtcgttttg gtgcattcat catactatcg atttcctcct
     2281 atcgttttcg ttgtttcatt tctcttcgat aaatcggttg tttttctcgt tttcgtttca
     2341 tttttgcttg agttgctcga aactcactcc gtgtcttcgg tgcttgtttt ggtgattttg
     2401 tttgtttcat ggttgagtat gctttagata cggttgaagg cgctttggtt tgtgctgtta
     2461 ctcgattgac tgctgacata tgttcccgaa ccccctctgg acgtcgaaca gatttcggtg
     2521 tggtagaacc tgttgctcgt ggtacattcc cttttcttag tggtttttgt accgcatgtt
     2581 tttgtcctgc aactgtccgg gttttcatcg aacttgtttt tgttacggga gtaagtgcct
     2641 ccttccgacg acgacttgtt tgtccctgac gacgttttcg atcgttcaat tctgttctag
     2701 gcgtatgctt ctggccataa gacttgtgtt tttgctttgg attcgctaat gacaccacca
     2761 ttgcagaacc ctgattgcga tacggactac gagacgcacc ttttctactt ctttctctta
     2821 gactaggatt gccttgcaat tctcgttgta ataaatcggc tgtttttctt gcctgtttct
     2881 ccattgctgt tcgttgcata cgttcttgac tttgctcttc tgcttgtttc gcttgatcat
     2941 gcaagtctct tgctttttgt gccatacgtt ccatacgatt tggtgcttta tgacgaggtt
     3001 gttgtgcgga ttcacgccga aaagcacttc gtgtggcttg tttcgccgtt tgtggcatac
     3061 gaacgtctga gccaccccca cgttttcgtt gacgttcttg tctcttccgt gcgttttctt
     3121 tttctttttg tttctgtgct tttctcgctt ctttgcgttc tttcaaccgt tgtttggctt
     3181 cccttctcaa ttttgcttct gccccaaatg gatttcccgc aaaagctaac gctcctcgaa
     3241 cacccattct tccagtaaat agactgccta aaaagcctcg gaacaaataa attcccaacg
     3301 gcgcaaacaa ataaacaatg atcaagagaa ttttttccca tggatcgtct tgtgtactac
     3361 ttaattggaa cccgtaacta agaaaagaag aaaaaaggat acatgcaaaa cctgtcattc
     3421 cttttaaaaa cgtcaaaata ccaaaaactt tggcatagtt accaaacaca ttcattgtga
     3481 caagaaacaa tcctaaaaaa agtaagaacg gcaacatcgg tactaacaat agttgcatag
     3541 ttccaataat caatcgaaac atacttaaga caaaataaac aacggtttga atcagattca
     3601 cgacaaaata aaacaaggca aatgctgtat ttttccaact attgtaatat tgtatgttct
     3661 tgtttttcag tgtatctgct tcatactctg tgactttgtc atgaattttc tcccctattt
     3721 ttgtacctaa atcattgtcc aataaaagat tgattcgatc atattctttt ccagcatatt
     3781 tgactttctt ctcacgaatt gtgtctacat tggttgtgcc ataatttaac aacagatatg
     3841 gttcataaac attactataa aagacccgag ccgccatcaa tttgcctgcc gtttccactt
     3901 ttttatccgc tgttacctct tctgtttctt ttgtttcata atttgtttct tcttcaggtg
     3961 ttaacactgg attgaccttc acgaacgccg tttccactgt agaatctaaa gaaaaaagaa
     4021 catcaaaaag attcttattt gtcccagaat cttttaatac gcttaaccca gtaaaaataa
     4081 ggatcgtcat aagaaagaca cggagtgctt gtttcattcg agcctctcca gcatatttga
     4141 ttgccatcat aattgccgaa aaactgattc caatagttcc tgcaatactt aacatattgt
     4201 ccgcaatttt ttgtgttaag ttttgaatgg gttctcgaat ggcggaaata atatctaatt
     4261 caaagagaaa ttcaaccatt gaagcattga attctcctaa catttttacg ccactccaaa
     4321 caaaattttt catcactcct gcaacattgt acgatagctt ttttttaagg ttcattgact
     4381 catagtcatt ggtcatcaat tcaaacgaat tctttttgta atttttatac aattgatcca
     4441 catcagtcgt tacttctttt acttctactt tatcgttggc attctctcct tcagaacgat
     4501 cggtttcagt gacacccgca ccatcattca tttgatcggc agaaacagta gccccttgta
     4561 gacagaaaaa aagaccccct aataaaagga gcgtccacca tttcagagat ttttttcgca
     4621 aaaaaaccac ctcttttttg ttttatcgtt gtttttcatt ttctaacatt gcttgaattt
     4681 tttcatcttg attttcagta gaagaaacag ccaataacca actatcaaac aacacatcaa
     4741 tggcaatcgg ttgatttctt cccaaatgat cctggaataa acaggttcct gtttgtaatt
     4801 gttccatcat ttctaaattg gctgaattca cttccatatc aaagaaggac aatagtttct
     4861 ctcgtgcttc tttttgtttc ggacgaaacg caaatttgta agaaattaac tcttttttat
     4921 cttcttgatc gtaatcttta aacgcttgca caacaaggta aatatccgta ttattcgcac
     4981 gtccttttcg caagctatca tcaatcaaat agcttccttg tgcggtatct tcataccctt
     5041 ttgcctcgtc ataaataatc gcagcgtctt cttctttgtt tgtagaaaag atatacgtaa
     5101 acttcataat gacttccata attgccattc ctgcaagttg ttcgctattt aatcgacccg
     5161 aatcaatctc tttttgtgtc ggaatgcgta ttccttgtgt ccctaacacg ttgatttgtg
     5221 aatcaaaacg tatcggcgta ctgtgctcat tcccaatgag aatctttcct aaaccaacgc
     5281 tatgccctaa aatcaattta gctagttctt tgtcactttt tcgaatttct tcgataactt
     5341 gacttaacga atgcttacct ggcatggtgt aaactacatc gatggcgtct tggatcaccg
     5401 ttttcttgtg gctcgctgtt tctcgattgg tatctacttc tcctaacatt cctaacacat
     5461 ttttagcggt ttgaattgcc tcttcttttg gtaaaaacaa tagaggatca agcatccctc
     5521 gacagctttc atctgaagat aacgaaataa aattaattcg ttcatacata gccacaaact
     5581 ctggtatgtg tccgaatttt tcaagtgcct ttaaaaagaa cttttgggtt tcatttttcg
     5641 gatcaatata aagaattttt tgacctaaaa acatcagcca taaaaaaata tattttacaa
     5701 ggaccgattt tcctttacct ggtggaccaa taatcaacgt atttccattg gtatgttccg
     5761 cgccttcaat acttttcttt gttaaattgg gataatacca tactagttta ctagatagcg
     5821 ctaacgcttc tttcgcagat ttaaactttt ttgcaataac aatccgtcct aaaggaaatc
     5881 ctttcttatt tcctacttct ttctttaagt ccaagccata atctgccaca aaacctgtcg
     5941 tagcgacctg ctcgtaacta cgaaaacgtt gcgtacttcc aattaagcat tgattaaaca
     6001 acgttagctg atccgctacc gatcgataaa atttgaaatc tgtagactca atcgcaaatt
     6061 caatatcttt gactcgttct tccaattctt cttttgaaga agcagataca accagcgtga
     6121 cggtagaata acacaatcgt ttttctttgc gcttcagctt atcttttaca tccattaacc
     6181 gttctgtacc aaaatttaat acatcgtctg aatctaaaat gccgtcaacg gtctcatatt
     6241 ctttttcttg ttcataaatc cgtccagcca ttttattaat ttttcgttca tcagattttg
     6301 aatgctcaaa tcgaagtcga atatgcgtat caattggaaa agataagcta tcctgaagat
     6361 tttgaataaa gctactccca tacatggcta aaggagcgtc cgtttgactt aaaaacgtcg
     6421 aataatgcgt ttgattcaac tgttcaatcg caatgtatcc tgcttcattc gaaatgacgc
     6481 cctcgcaaat ttccattggc gtaagctgtc tttcaggcaa tttttcttct gatcgatgga
     6541 aaaaataata ataaatttgt ccaatctctt gggcagataa gcgctctatc ggcttataat
     6601 tggacaattc cgtatagagc tccttttcta atgtttcata cgttggtaac acactactta
     6661 taggaactcg ttgtcccgta atacgataga taaacttttt aaaaacatgc ttaagcaatt
     6721 gaaaatattc gatcggatca gacactttca tttctgtagt gaaacgcaca aacagatacg
     6781 ttcgatactc gtttaattgc acttcgtctt ttaaaatctt tcctgcctgt tcaaagtaca
     6841 ctttcccaag atctgcgcat tctcccttta ccaaatctcg aatggtttct tcaatgtgcg
     6901 tatctaggtt aaattgacgt ggaatattga taaaatgata gtcatattca tcatgtccaa
     6961 gtatgccttc gccatcttga agatagttct tgaaaaaatc aatgtcattt aaaggaaata
     7021 cttgggaaga aagttgatag cctacccaca catcatcatt tttggtcaaa acaaaatttc
     7081 ggataatggc gtctatcgga aattctaatc gaggaagctt cgtttttttc tttttgaact
     7141 gccaaatccc gataataaag acgaccaaaa gaaacaaaaa cgccatttac tcgactcact
     7201 ccttccattt gtgtagggaa tacgtcactt tttcatggaa aaacggcaca tacatttcat
     7261 gttcaaagcg ttgtttccgt ttctgataat aggacgttcg cccaattaaa tactcgaaaa
     7321 agtgtttatt gtcccatttc attgaaaaca atgtccaaac gagaacacca atcccaaaaa
     7381 tcataatcaa ccaggacttt cgaaacagtg tctgcaagaa actaatttga ttcacatacg
     7441 caatcaaaaa taggatcaaa agaatgaaga gaaatacccc acctatgact aattgttcca
     7501 tgcgaatgcc attagaaatc cagggaattt tttgtccttt aatcccgtaa attttgtgag
     7561 ggtgcttaaa ctcctttgta tagttgtatt ccatgttcac tctacctcct tttacaaaaa
     7621 aagaagcgac cagaagttct aatcttctga tcgcttttgc agtttgtctt tttcttatga
     7681 taattgtatc cagggcacaa tcatttgcgt tacccccacg ttctcttcac tatccttaat
     7741 aaagataatg ttctttagcc attcaccaat atcaacggca ttcgataaca ataagacgag
     7801 cattatcaaa ataaagacgc caataatggc aaacgctgcc cgcgcaaagc cttgggtcgc
     7861 ataagcacca accattagaa caatcgcaac gattgccaat agccaaatga cttgtccaga
     7921 tacatcatca aataacgatt taagatcaaa aagtttcatt tccattctcc tctaagttca
     7981 ttttattcat ttaacgtagt aacaaaataa ctatcgccac ttgtttttac ttccattgaa
     8041 aagtttgatt ggaactcgtt tcctttctca aaatagaaac gatacttccc ttgcaaaaag
     8101 tagtttcctt ctttattttt tactgcatga tccagttgta cattgatcaa ttcgccgcct
     8161 tctaaccctt tttcgttaga aatcaatgcc aatttttcat catttttgac atataactca
     8221 aaaaaccgtt tcacaaatgc ttccagcttg atttcttcgc tttcagtgac cgcttgccca
     8281 gaagaataaa atcgattttc ctgatagttc acagaattgg tattcttact ctcagaaaga
     8341 ttcaaaaatt gaacgccatc aagtactttt aaaacattgt tttgataact gactggaaaa
     8401 gaaagttgac tgcttttcca atgttctcct tctgtaaaag taacctcata aaataggcga
     8461 tacattgctt gtgtcaaatc gatttcttct agtttaataa agcgaacatc tttcgctact
     8521 cgattggtag ccgtcgttac aaagcctaga tcagacgctt ccaatccttg tgctaaataa
     8581 tgattgacgg aagcctccca ttcgtttcgt ttttctggcg aataagtaaa taacctttca
     8641 ataaaatgtt gtccctcgta cttcaattgt tccgtagaag acaaggtttg gttgacttca
     8701 gttaacaatg tttctggatt gacttgtttt ttcactgcta aacgcgttaa tgtatccact
     8761 cgtccaaagt ttaggaggag cacgacaccc attaacagaa acaaacaaaa aaaagcgcct
     8821 gaaaatacac tcatggcact tttttgcgaa aattggatca ttcgatctga cggttctgtt
     8881 ttcttttttt ggaacaaaaa acgtttcttt tgtttctttt ccttctgttg tttcggtggc
     8941 ttagtttgga acgcttgtgg attaaatttt gcattactca tgagaaatgc cataccgttc
     9001 taacaataat ttcggtattt tgtccacaat cactacttcg tggacaacca cacttgcccc
     9061 ctc
//
LOCUS       DARDHA010000111         8995 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000111 DARDHA010000000
VERSION     DARDHA010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.112"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            775..1248
                     /locus_tag="SDA10_002049"
     CDS             775..1248
                     /locus_tag="SDA10_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEG4240940.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1257..1484
                     /locus_tag="SDA10_002050"
     CDS             1257..1484
                     /locus_tag="SDA10_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4240941.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2120..2491
                     /locus_tag="SDA10_002051"
     CDS             2120..2491
                     /locus_tag="SDA10_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240942.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2747..2989)
                     /locus_tag="SDA10_002052"
     CDS             complement(2747..2989)
                     /locus_tag="SDA10_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG4240943.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3021..3923)
                     /locus_tag="SDA10_002053"
     CDS             complement(3021..3923)
                     /locus_tag="SDA10_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG4240944.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3936..4124)
                     /locus_tag="SDA10_002054"
     CDS             complement(3936..4124)
                     /locus_tag="SDA10_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEG4240945.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4138..4701)
                     /gene="amaP"
                     /locus_tag="SDA10_002055"
     CDS             complement(4138..4701)
                     /gene="amaP"
                     /locus_tag="SDA10_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEG4240946.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4739..5632)
                     /locus_tag="SDA10_002056"
     CDS             complement(4739..5632)
                     /locus_tag="SDA10_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG4240947.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5710..6633)
                     /locus_tag="SDA10_002057"
     CDS             complement(5710..6633)
                     /locus_tag="SDA10_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG4240948.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6682..7032)
                     /locus_tag="SDA10_002058"
     CDS             complement(6682..7032)
                     /locus_tag="SDA10_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG4240949.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7065..7967)
                     /locus_tag="SDA10_002059"
     CDS             complement(7065..7967)
                     /locus_tag="SDA10_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEG4240950.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7960..8817)
                     /locus_tag="SDA10_002060"
     CDS             complement(7960..8817)
                     /locus_tag="SDA10_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEG4240951.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
BASE COUNT         2512 a         1715 c         1490 g         3278 t
ORIGIN      
        1 ggctatttga ttgtatcaaa tctaggaaat atggtatttg gtatcagtaa agaattcagt
       61 tggactacta cattatggga atctctattc tttttcatct tcgtattttt gttacaaaat
      121 tatcgaaaaa aataaacatg tgccatctat ctgttggcac atgttttagg atttatggta
      181 ctttttattc ggcaatccat tttatttttc tacttcttct taatacctct aacacttttg
      241 atttataaac taatccaatg catctacttg cgcttattaa gctatttcag ctagatagta
      301 ccaccccatg cgtcaatgct tgattttcct tttgtataac attgtataag caataaagga
      361 caaataagtg gtgattacac caaaccattg aataagaaaa attctgatac gtaaataaac
      421 atgaacttga aaaaattaat gaatcggtta gctactacgt agttgtcttt tttctcatac
      481 agttctgtta acttacactt ctcttcgcta gataataaaa aatcctccaa ctgtattgtc
      541 caatataatc ccaggcatct aaactagcat tatagaagag acacacatag aatctaaaat
      601 aactggtttt aaatgtttct tacaatcaaa aaaagctgtt aaatgcactc cacaccttca
      661 tactctatca ttttttataa acgatatatt tttattgttt ttcgataaaa aatagttgtt
      721 ttacaataat tattctgcta tattttagct atcaaagata aaagagggat aaaaatgaaa
      781 tttaaaacat tgctattaaa atttgtcgtt ttaggtataa gtgccgtatt tattttgttc
      841 ggcgtacttt tttatactca gatcgctaca agcgaaaaag gattcaattt tgattggcag
      901 accacattat ttgtttctgt catttacttt gcccttctcc tagggatcgt tattgcttat
      961 ttcttattga aactattgaa aaaaatcgaa gataaccaag ctttttccac tcaaagctta
     1021 aacatactaa aaaaaatcga acgaaccatt ttgtttattg gtttcgcctt ttttggctta
     1081 cttcctaaat ttatgaaagt agcggattta gacgatgctc caggtttgat gttggtagga
     1141 atcgctgtcg tattcattcc ttttgcagta tacacattaa caagcgtgct ggaaaaactg
     1201 cttgaacatg cgattttact taagacagat catgatttga cagtgtgagg tgcgacatgg
     1261 caattatcgt taatttagat gtagtattag ctcaaaaaaa aatgagtgtg actgaattga
     1321 gtgagaaagt gggcattacg atggcaaaca tctctattct aaaaaatgga aaagcaaaag
     1381 ccatccgttt ttccactctc gaaaaaatct gccaagtttt ggagtgtcaa ccaggtgatt
     1441 tgttgaccta taaaaatgac tcgaatacta taccagaaga ataaaataaa gtagaacaaa
     1501 cagtaaataa aaaataatct gaatgataga tgtgtgagac ttttagaagt tatttacgtc
     1561 tattattcag attttttatt tcgattgaat accgttttat ctataaataa gatatgtact
     1621 gactcataca aagggggcct tttcttctcc atgtactatc acttttcctg atacaactgg
     1681 tttcctgtta cgttgtagtt ggatctgctt ttagtataat actttattca ttccaccaga
     1741 ctgatagcag tttgagcagt ctaaataatc atgtagtatt acaggccatt ctgtttcata
     1801 ccttgaagtg cttaaaactt cagctaatgg cccattgtgt taactcttac tccattcttt
     1861 tttagtaagc tcccatatat actgtaaagc cttctatata ttatgtcatg ttgcttttta
     1921 acatttacta tgtagttttg ataaaatgat ttgaataaca gtgatggaca attctttttt
     1981 atttttaaca ttattttgat aataatgatt atttatttaa aaatacatta gataacattt
     2041 gttacgacac gaaaaagctt cacaaacaaa tatcgttatt ttaaaatgag ggcgaactca
     2101 cacaattagg aggaatagta tgccatttat tgtttacttt tttctttcat tactcgtgat
     2161 ctatattcca ttaccaacat ttttcctgct cgtcaatcgt ctagcagagc aacatgatac
     2221 gattgctgct cttacgaaac tttgtttact tctatttgtt tgtctagatt actttacgag
     2281 ctattattgg ctaaaacaga caagagtaaa aaagagatat tatttcttcc ttgtcttttt
     2341 caatctcttt gagtttctga tccattgctt tttacttcat cagtatcata cgatccattt
     2401 gtggatcatc tgggttttca aagtggtcgt attattttgt atgttcacct ttcctatctc
     2461 aagaaaattt agggaggcat tgttttatta aagaagcttc tctgtatcaa taaagacaca
     2521 tttaaggaaa aacttttaaa aatctaattg ggaatttgcc cgcataaata agaggctgtg
     2581 acaaaagtct tgtcacagcc tcttattccg tataaacagt gttcaaaagc ttgcatcttc
     2641 gataatatgc ccaaccaaat gaaaaatatg agaagctatt tttgtttggc tgttcttatt
     2701 acttgatgaa gaacagcttt ttcacgacct ctttttaaaa gtctttttac agttttcgag
     2761 caaagaatga tacgattagt actaaaatga tggcaccaat caatgatggc acgattgcca
     2821 ttcctgcaat acttggtccc caagaaccta ataatgcttg accaagtgct gatccaacta
     2881 aacctgctac gacatttgca ataatcccca tcgaagaacc tttattcgtg attgctcctg
     2941 caattgctcc gataattcct ccgacgataa gtgaccaaat aaatcccatg tttcgttcct
     3001 cctaatgttt tacttcctta ttactgaacc cgtggttctg tattttcttt tactttttgt
     3061 gcgccgcttc cagctgctga ttttacttta tctgtttgat tcgaagcaaa ttcaccggtt
     3121 tttttcgctg cgtcagttac tttgtcttgc accgtttctt gatctttttc aaattcttct
     3181 tttgttttga tgtccactac gttagcgttg acttcgatca cttctaaatg tgtcatattt
     3241 tttacttctt tgccgattac ttcttgcatt tcttcaaaga ttttttcgat atctttgcca
     3301 tattccacaa tgacatccat atcaactgct acttgttttt taccgacttc tgtttcgatc
     3361 ccagccgtta cattatctgt attcacaagt tttcctgcca cattagagaa gaaaccaccg
     3421 tttgcagata ataaaccgtc tacttgttca attgcgattc cgatgatttt ttgcacaact
     3481 ttatcttcat aattcagctc accgcgcatt gaatcttcct gttcagaatc atgctttcct
     3541 tggttatttg tatgatcaga accaagtttt ccttgtccgt ttgtgtggtc agaactaagt
     3601 ttttcttgtg ctttcgcatg gtcagaagga tgtttttgct cttccacagg tttagaagga
     3661 tcgattgcat catgttgttc tggaacttta gattctggag ttcctggatg atgaggtgca
     3721 tgcttttctg catcatgttt cttctcttct actccttgtt gaacgttagg gactactgga
     3781 tctggatgtg cagatactgg tgctgttttg ttcttttcat cgttcacacc atttgattgg
     3841 tttggtgtcg ttgggtctgg atgtgcagac actggttttt ggttgttcat tttattgtta
     3901 ttatttttgt tattgttatt catggatgtt tcctcctaag atttaaaata gtgatctaag
     3961 attttcgttt gtttcagata aaaaccgata gcgacgccga tcgctgccat gatcaagatc
     4021 aataaggtct tgaaaaagcc cacggtgatc cataaaacgg ctaatatcaa tccaagtacc
     4081 ccaaagatga tagggagctt gtatgtcgaa aatagttctt tcatttttgg tcccccccta
     4141 ttctactcgt ttgcggtcat tgcttcgttt agctggttct tcaaagcctg tgtatttcac
     4201 cgatactttc actggctctt ttgttcccaa tatgtgttgt acttcatttt cgatttcttt
     4261 catcaatgtt cctgttttac caatgagtgc tgaagttcgt ttcaactccc cttttacatt
     4321 gacggcgatc ttgtttttcg ttgctttgac atttactttt ggtgaatgga tgaattcatc
     4381 ttcatgcaag tgagaacgaa ccattccttc aattgctttt ttatccaacg tcaactcccc
     4441 atcaccatgt ttcagtagaa agtttccgat tgtttttgga taaaacagga aaacaagtaa
     4501 cgtgataatt gtcaaaatag caaatgcaac ggcaagccaa aatacatatt gttgcacgta
     4561 tgaattagtg atggaaaaga atcgaaactt ctctagctga aaaggcaatg gataggttgc
     4621 acttaatagt atcgcaaaca aaagcacaga tagcaaaagt aatgttccga tcactccgat
     4681 cgtttttaca cctttattca ttttcgcacc tcctcatagg ggtgaaacga tcaacttctt
     4741 ataagttgat cgcttctcca ccagttactc cgtaaatttg ggctgtaaca taacttgctt
     4801 tattcgatgc taagaaaaca taaactggtg ctaattctcc tggctgacct gcacgttcta
     4861 gtaaactttg ctgaccaaat tcagggatcg aatcagaagg ttgtccttcg tctagttgta
     4921 atggtgtcca gattggacca ggtgctacac catttacacg gattccttta ggtgcaagct
     4981 gttttgctaa accgatcgta aagttagcga ttgaagcttt tgttgctgcg taatctaata
     5041 aatgttcgct tggattgaat gcttgaacag aagttgttgt tacgattgct cctcctgctg
     5101 ggatatgcgg aacagcttct ttcactaaag caaacatagc gatgatgttt atcataaatg
     5161 tgtctttgac ttgctcaagt ggtaactcag caatagactg acaagaaatt tgctgtcctg
     5221 cgtttagtac catcgtatct agcccgccaa gttcactcac ggctttatct atgatttctt
     5281 taggtgcttg ttcgtcacga agatcaaaag gtagtaagac aatcttacgt cctgcatctt
     5341 tcacgtattt tgctacttcc tctgcatctt tttcttctcc tgggaagaat tgaagagcga
     5401 tatctgcccc ttctctagca aatgcaatcg ctgctgcacg accgatccct gaatctccac
     5461 cagtgattag tacacggcgg ttttctagtt gattattgcc ttcataggat tcttctccac
     5521 aatcaggaac aggaatcata ttctgctgta aagctggtgt atcctgatct tgtttaggaa
     5581 atttctccgt atggtacaag tttcgcgggt cgattaattt tggttctgtc attgctgtat
     5641 tcctcctagt ttttaaattg tattgccaaa acaaatttct ttgtttgata cctgttttaa
     5701 atagattctt taatttgtat aatctttccg gtataaatgg taacctaaaa ctactgctgc
     5761 tatcaaagag ccaaagacta cgagtataaa gcctagagca gaacttttac ccggtaatcc
     5821 tcctgtattc attccccaaa tccctgcgaa caacgccgga acggaaatga ttaatgcagc
     5881 tgaatctaga tatttcatga tatgatttag attattgtcc atcatcccgt tgaatagatc
     5941 accaatactc tctaataaat ctcgatagat ctcgatcatt ttttctgttt gtcgctgtct
     6001 caaatggaca tcatagagta gctgttcatc gtctaaacgt ttgatgaaag tttcagaatt
     6061 ccataattga tccattgttt cccgttgatc tcttaaggta tgatcgatat atacgatgtc
     6121 tctttcaaga tctgcctgtt tatacagttc ttcattttcc gttgttttac gagctgcttt
     6181 atccaaagcg tcaattcgct ttttcatctc tcttagttct ttcacgtaat gggtgtaaat
     6241 atctaataat gtatacgtga tgatcttttc aaacgagttt aaatgatttc catgctttgt
     6301 cagcagccga tccacaaatt cggtttttct atcgatacaa gtaatcaaga gattatcgga
     6361 aagtacaaaa gatattggcg ttaatttttt ttcgatacga ttatctgact gatctagatt
     6421 aatcactacc aaagaaaatg gatgctctag tttcgtctca taaaggcgct ccatccgaga
     6481 aacttcttcc ggatattcgg ttcctacgaa aatatcatca ggaagctcga attcttcgat
     6541 tgcttgctca atctcttcgg gctttgcact atcaagtacg agccaattat attttttttc
     6601 atctgtttct attacattct tttcttcatc cacaaagtag tatctcaatg tatacctcct
     6661 ttttggactt tctcgaaact attagctgaa gaagcagttc tttaatttga tattataatc
     6721 gagtactgcg atttcatttt ctaatgaaaa gctgtcaagc tgtttgttga agtccatgtt
     6781 caatcgttta ccgaaataat tttgctctaa tttagatgta tagatgtcca ataatggatg
     6841 ctcgatttta gtcgcatatt ctccaaattc cgaaacaaaa ggaatgattt gaaaaaagct
     6901 gccttttgca aaagcacctt tgtcccttag aaatggatcg gaatcattga taagcgcgag
     6961 tgcgattttt gtattttccc caaccttttc aattaacgtc tctactgcat ttttagacat
     7021 tgtaatttcc attcctactc cccctcttca gttgttgtct tctattattt aacaactaca
     7081 tctgtttttg gttgaacttt tgcatatttt ggatatttag ggaatttagc tacacaacgg
     7141 aaagccgtca tccctgcata tttttcgtaa tatttcttcg ttggcccttc aaaatccttg
     7201 ctccacggtt tcacatgtcc tgaaaaatgg ataatgtaag gattgttgcg agtttcttcg
     7261 tattcacgtt cacctgctgc tgttggatgt ttttttgctt ttgccatgat atagccttgt
     7321 gcattccaac gcggatgaag tggtaaccaa cggtcatgta agatagcatt taatgcatct
     7381 tggtcatgga atcgtaattt ttctggatta tgttcgatga aatctaatac tttttgcgta
     7441 atattttcat ctaaccattt tttcacattg atcagcatca aacctgagtt gaaataacgc
     7501 attgatttag ctgaaatttt cattttttcc agtcgttgat ggaacccagc gtcttcaaca
     7561 gctgctacga ttgaatcacc gaagtcaaga cgccataatt tagagatatc ttgtaaagcg
     7621 atcatgtcgc aatccatata taagatacgt tcaacttctg tgccgcggaa caattccgga
     7681 atcgcaatac gataataagc tgtttctgga atacgatcgc tgattaccac atttgtaaag
     7741 aattctttat tgattttcaa gaattccaca tctgagttca ttcgtgcgtt tttcactgag
     7801 aagcgtaaaa gctgtttact ttcaaaatcg atatcatcgt caatcacata gaaccgaaca
     7861 aatttagacg ggttgcaatt atctaatact gagacaaata atgcagctaa atgtggtacg
     7921 aattttgtgt tacaacttgc gactactgct atttcttttt tattcattga tctcgcctac
     7981 tttcgttaga gtactgtgtg ctaatttttt taagtataaa ttggtatacg gatgatcttt
     8041 caacgtattc caaggtttat catgtcctgt aaagtgaacg atcgaaggtt tttcatttcc
     8101 ttctttatac tgacgttcat atttttcatt tggtgctgga tgacgttcaa aaatcagcga
     8161 agtctgcatg ttccattttg gatgaagttg ttcccaatct tcataaagta cagcattcaa
     8221 agcatcttgg tcgtgataga tgattcgatc gccattttca ctgaggtaat ggatagtttt
     8281 ttctgtaatt tcttttttat tccattgatc aatgtcaatc accatcactc ctgaattgaa
     8341 gtacaagtca tcggaatcga ttcctaatct ttccaaagct ttcacttggc ctggatcgat
     8401 aatagcgccg attgtttttc catttaacgg ctcatcatat agttttacga tatcgtctag
     8461 tactaacaca tctgaatcta agtaaaggac tttcttataa tcctctttag ctaataagtt
     8521 tggtaaagaa attcttaagt aagcggtagt tgtgatatgg tcactgacta ggaagtcttc
     8581 gtagatatct ttttccactg tcaggaattg gatacttgca ttacttgaat atttatttac
     8641 cgtttcttcc aatccttctt tactatattc tgatagacca tcgtcaatca cataaaattt
     8701 tatgcgtctt gttttattgc aattttctaa tgcagttgcg atcatcacgc tcaagtaagg
     8761 agcataattt tcgtctgaag cagttaccac aggaactacg ccgtatctct tctccatcaa
     8821 aatcactcct ttttctaatc tttttctaac ctgttctaca tctagaatat tcctcatttg
     8881 gttttaaagc aaaatatatg ctttgttttc attgaatatt ttaaaatttt tttatcacaa
     8941 aacgcatcgg taagcgctaa ctattcgttt ttcatggctt tttattcaaa ggtat
//
LOCUS       DARDHA010000112         8914 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000112 DARDHA010000000
VERSION     DARDHA010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.113"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            305..799
                     /locus_tag="SDA10_002061"
     CDS             305..799
                     /locus_tag="SDA10_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4240952.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(961..2133)
                     /locus_tag="SDA10_002062"
     CDS             complement(961..2133)
                     /locus_tag="SDA10_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG4240953.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(2339..3154)
                     /locus_tag="SDA10_002063"
     CDS             complement(2339..3154)
                     /locus_tag="SDA10_002063"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG4240954.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(3304..3750)
                     /locus_tag="SDA10_002064"
     CDS             complement(3304..3750)
                     /locus_tag="SDA10_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEG4240955.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(3747..4751)
                     /locus_tag="SDA10_002065"
     CDS             complement(3747..4751)
                     /locus_tag="SDA10_002065"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEG4240956.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(4791..5120)
                     /locus_tag="SDA10_002066"
     CDS             complement(4791..5120)
                     /locus_tag="SDA10_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEG4240957.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(5246..7573)
                     /locus_tag="SDA10_002067"
     CDS             complement(5246..7573)
                     /locus_tag="SDA10_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG4240958.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            8215..8328
                     /locus_tag="SDA10_002068"
     CDS             8215..8328
                     /locus_tag="SDA10_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX protease"
                     /protein_id="HEG4240959.1"
                     /translation="MYAPGTILLTIAYLTANRSLAFIIAIHILNNVLGFVL"
     gene            complement(8416..8709)
                     /locus_tag="SDA10_002069"
     CDS             complement(8416..8709)
                     /locus_tag="SDA10_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEG4240960.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         2490 a         1720 c         1629 g         3075 t
ORIGIN      
        1 tattcataac acaattatta taacaagtaa gtaaacaatt gaaaaggaca tttattgaaa
       61 acggttgttt tttacttaag gaggaaattt agtgaaaaaa ttagttgcgt tgtttttggt
      121 cgcaggaagt ttgttgagtg ggtgtggtgc tactacgtct actactcaaa gcacaagttc
      181 agctcaggct tccagcagca gtaccacgat ctccgaaagt acggcagatt catcgaaaga
      241 acaaattgaa aagaaaaaag aagaagagaa acaaaaagcg gaaaaagctg cactagaaaa
      301 aaaagtgaca gaagcggaat ctgctttgtc acaagctgaa gcaaatccta caaaagaaaa
      361 ctatgcaact gcttcagcag ctatccaagc gctacctgaa aacaaacaag agttgacaag
      421 ccgtcttgct gcaattgatt ccacaatcaa agcaaaagaa gcagaagaag aacggaaaaa
      481 gcaagaagaa attgcacgac aacaagcaga acaagcgcaa caagaagcaa tcgcacagca
      541 acaagcagaa caggcgaggc aagaagcaat cgcacagcaa caagcagaac aggcgcgaca
      601 gcaagcagca gctgaacagg cttctcagca ggcacaagcc gcagctcctg agcaaaataa
      661 tgaacaaact gtctatgtca caccgaccgg ttcaaaatac catacacata aatgtggaaa
      721 tggtacgtat acccctgcca ctttaagcga agctcaagct cgtggtttaa ccccttgctc
      781 caaatgttat ggaaactaat tcaaaagtta tactataata ataaaattct tctgaaacat
      841 aaaaaaatag aagctggcgt tccagatagt aaaccaaaaa tagcagttta cacgtccatt
      901 tcgtcagctt ctctcttttt tcatttagtc tgttccctct gcttttttgg acatcttcat
      961 tcattctttc tcaatcaaat agtacacctc tttttctttt cgcagtttat atcttccttt
     1021 tttcaatttc attgaaaact gtttcgaatg tttatgtttt acatggattt taagcaggag
     1081 ctcctcatgt tgcaagcgat agttcctgcc ttttaaatca gaaaatctca ctagtccgta
     1141 ttcatcagaa gctttcacaa atggaacttt ttctctagta atatagtgtt tgccgttgag
     1201 cgtttttaca gaaaagacca ttccttcgat tggtcgtccg tttgtgttct ttacataaaa
     1261 agataaagga tagtttcgct tagcgatggc caaagaatct aaataagcga tgagtagcca
     1321 aactaagccg attgatccaa gtagtatacc gataaaccaa ataaaaaatt tccatttttt
     1381 ccattgatca attccttgga tcgctttttt tgcctctttt gcttgataag gaatacgatg
     1441 ccccgtcaca agcagtcgat gtgtattgac catataagga gtgcaggtaa gcaaagtcac
     1501 gagatctctg cctttttgga tacccaaaga atctacttcg tcaggtaaga ccacttggat
     1561 tttttctact tgatacgcca atgtctttcc attgatctgg atgaaaaact ggtctccttt
     1621 tttcaactta ggcaaatctg taaataattt tgcttctggg agtccacgat gcccagaaag
     1681 aacggcatgg gtgtttttcc cgccagtcgg ataagaactt ccttccaata atgacgttcc
     1741 tttttgcaaa aatatttccg ttgtctgatc gaatataggt aagctgacat tgatttttgg
     1801 gatttcgatg actccaagca tatgttccat aaaaaaggca ttactaggta aatcttttaa
     1861 tgcttttgca tcaactgctt ggttgaaact gcctatccct ggtgatacgt tttgctctgc
     1921 caactgttgg tttttctttg tcattttttc ctgctgtttt tttatttcct ctgaattctt
     1981 attcgatgct tgcttttgat aatggatgat catttgctgg gcaagaaaat cattcagtga
     2041 atcttctaca aaaggataag aaaagatccc gatcccaacg actaaaatga ttgccatgaa
     2101 gatgtccagt attcttattc tgcttttatt catcatgtcc acctattttc taatatgtat
     2161 atgaagaggt tgtgaaatca gcgccttgac ctgagtatta gcgaagcaaa cggcaaaata
     2221 gttcctccta ttttgccgtt tgttgagcga ataccaagag accgaccact tgtcacaacc
     2281 tttctcccca tttgaatcac tatcgtttat ttatcgtatg atttttgttt ccaatagttt
     2341 aagcttctgt ttcttgatgg cgttttttga aatagatcac tgctcctcca actaaagcag
     2401 tacctaccaa gatgaatacg atgatcccca taccaccagt tgaaggcaag attcctttag
     2461 gttggttatc gatcgttgtt tcatcagaag ttgttggatc atcatctact gtaaattcag
     2521 tgtacgcagc ttcattctta ggtaatgcgt agccttctgg tgcttttgtt tcttgaaggc
     2581 agtatgcccc ttgtttcagc ccttcgattg aaaatttccc ttctgaatca gaaatcaatt
     2641 tcacttcttc ccagtcagaa cctgtgactt catctgacca agcatatggg tattctcctg
     2701 aggcaaggtt tgctattttt ttcattaatt taacagttgg atcttttgct cttagaatag
     2761 aaaattctgc atcggctaat ttttcttgcg tgttcacatc ttgtttgatg aagtggaaat
     2821 caccgtagtt ttggacattt tctacagtaa tcgtgttatc cgtagttgta ttttcccgat
     2881 tgactacacc gtttggattc ccaagattgg cttcatctgc gccatggatg ataccggttg
     2941 tttgttgcaa ttttccgttt tttactataa aagtagtttc tttttgtgga ttggttaatt
     3001 gataaccatc tggtgctttt gtttcaacta aagtataatt gccgtctatt aatccattga
     3061 cactgatgga tccttcagta tcagttgtga atgtcaacgc tgactcagca ggatcataga
     3121 gagcgacacc agtgttttca tcaaaaccgg tcacgaattg attctctgaa tttttgatct
     3181 ggaattgcgc ccctgctaac ttatctaccc ctgaaaagtt cccagctttc tggattttga
     3241 tattgctttc tttgatgaca tttttagcat ataggtgaat cgtgtctaaa ggtttgtcag
     3301 ggtctagggt aagcttaaca cgatatttgg cgacgtttcg acaccgggct ttggtgtttc
     3361 tacaaagacg tatacagcat ctttcccatc agatttacct ggaagagtca ggttagctaa
     3421 tccttcacca gaagtcgtta cacttgcgaa gggtgtagcg tctgctggaa ctgctgtgtc
     3481 tttacttgga tagtacgtag cagtgatgtc atatacgttg aattcaacgc cgttcaacgg
     3541 ttcgctattc ggaaatgtca attccgttcc gttgtttatt tgattttctg gtgctgaagt
     3601 gaatttcaat ttatgcaaat taaccgtaac attttctggt gtaggtgata cggctgcaga
     3661 aacctctgca gttcctaggc cgagaacgaa aggaagcagc atgatagcag ttcctaaaaa
     3721 cgtccatatt tttgttttga tgtttttcat tttatttccc tcctattgtt tcctttttgt
     3781 tgttttttat aaaaccaagc tgtccctgtt gaaaggacta gcaataaccc taaaattgag
     3841 aaacctccaa gcatctcttc gcttgttttt ggaaagcttc ctccattttt ttcatttgat
     3901 ggaccatttg tctgcatctg aactggcgtt tttccattat tcgtcggatt tgcttttggt
     3961 tcgttgatga tttccaatgg tataggatga agcggatcat aactgccttt ttcaacttca
     4021 aagagaatgg ttgattgact caatatatat cctttaggcg gtttaatctc cactagttcg
     4081 taacttccaa aagacaatcc atggacagaa aatgttccat ctttactgga agtaaaaatc
     4141 gttgctcttg aatcatcttt ggctacccaa tgatttatcc cgttttcttg cgctaagtat
     4201 tggtcatttt ttttgactag aaattgagca tctgctagtt tttgtttcgt ttgccgatcc
     4261 actttgacaa attgtttgcc tcctgtttgg atggcaagat gtgtttctac tgtttgattg
     4321 tcagtcgtca aacgtacatc gttatcaaat gtcgtttttt cactgttttc tttcaatgtc
     4381 atttggtaac ggatcgttaa tttttttcca gcaaacgaac taagatcttt tccagaatct
     4441 gataagacaa aaccatgttt tattttttgt gtgtgaaatg tatgaatctt gtctccacca
     4501 atcaaaaaat cgatcgtttc tggttctagc cataactgag gatcagctgt atctttgatc
     4561 gtataggttc ctatctcgtt gatgttctca ggaattgtta cggtgatgag ataagggact
     4621 ttatccccat ctgcaaaatc tgtctgctta ttctctactg tttttgttaa tgtgagtgca
     4681 gcaggtattt tttgttcgct ctttggatac aagtggatgg tcgtcaattt atgattagat
     4741 gaatctgtca caggaagcac aacgacaaat ggtgctgatt tttcttttac ttaatctgaa
     4801 gtctttattt ccgtaaatcg gtaaacagcg tcacgacctt gctcatcttt gtccgatgca
     4861 ctaaaagaag caatgccctc ttcctcgtct actgtttttg tcacattttc tgccagtatt
     4921 ttttcactat cacttttttg tgctagtttt ctttgtgcgt cttccacact acttccttct
     4981 gaccgcagct tgtaaaattc atccgagaca tcatatatag cgaaagtaac gccgttcaac
     5041 ccttttactc cttgaaagtt atccgggata tttattccgt cgttttgact gatacttggc
     5101 atcttatcgc tttcgaacat tcgattatgc aaagtaaatg taataggtga ttctctagct
     5161 gacacagtcg gagaacctat aaaaaagacc agtaggataa aaaagagaac agcaaatata
     5221 tccacacgta cattttttgt ttttttcatc gctgttcacc tgcttttttc ttacgatgga
     5281 taaaataaaa gaagccagaa aaaataaaga tcagtcctaa aaagacaaag ggcagtagac
     5341 ctatcccacc tgtcgaagga agcaagagag tcggattgat cgtcaactta gcggtatctg
     5401 ttgcgataat cgtataatta ttgtttacac ttccttcgcc taaactaatc gttactattt
     5461 gtgatccaat atctgtccgg ctgttaactg ttagggtata atccaatgat tcgcctaaga
     5521 caacaccggt aacgtggaga tttccaaccg aaatcgagtg actttgggca tcataattaa
     5581 aggcagcgtc atcgacagta atcactgcgt tagctttata gatggtataa gttcctgatg
     5641 tgttggctat atcagtaaat gacaacttgg gatttgctgc tttgactttg tctattccgc
     5701 tattcgttag tttaattgta tagctgccag cattaaccat attttttgct gattgtgcat
     5761 ctgctgtgta atccatactg ctaagtgcca cttgtactgg agtaagcgaa ctagtgacac
     5821 cctctgctag cttgacatgg attgacggga cacctgcttt tccatcatat gtcttactgc
     5881 cactgagctc taccgcaccg ctgatttgct taatcgtata gacatcagat gtctgcacga
     5941 tagtaaataa atagtttgtt ctggcagcta gtttagctag tccgcttttt gtcaattgcc
     6001 aagacgtata ggttccaatt tcagtaggcg cagttacttc aactcccttt tcattcatcg
     6061 ctttgaaatc agttggattc catgtgattt gatcaatact gagatcattg tttaaaaggg
     6121 atgttgattc tgttttatca ttaaaggcat atttaacgat cggtacaaca gaaagaccat
     6181 catagtactt actgccattt gcattgattg atactgttgc atttgtctta taatatagga
     6241 tgacttcaga aatctgtttt ccatttgcgt cctttacgat tacatttcca tttgtatcta
     6301 aactagctaa aacactcatt gctgattggt tctttgtttc tatcgctgaa acagctgtaa
     6361 aggtatatcc gttgatagta ggcgtattag tcgtcaggtc ttgtgcagca cttccgtaag
     6421 taagattcat tattttagaa ggagcaatca tctgtccaaa atagtcttga tacttaatag
     6481 tgattggtgt ttgtttgcct gtataggtca ccgtatcgac gttactttct agggctgtga
     6541 aagaaccgtt tgtttgtgca aaagaatagc ccgttactgc tgttggagtg ataaccactt
     6601 tgtcacccac atggattcca gataatgtag tcccgtacgt tccgattttt gctgctccac
     6661 cattgactgt ataagaaaaa gttcctgtcc gattcgcatt gacctgaacc gttgctgctt
     6721 gataggttgg aaaatttgaa acatttccct taccatcaac gatctgccca tttttgatcg
     6781 agtaccccgt gaatggaata tcatttgcta actgtatacc gattgaaaaa gtactcatac
     6841 ctcctccggc aggaagccct ccccatgttg agggtgtaaa agaataactt ttatccgcat
     6901 tgggtgtaac tgaatcgatc gtatttccag cggtagccac ccccatatag atactttttg
     6961 ggaatgtgat agtagtacca tttactgtgg agccaataaa cagattatgg atatctggac
     7021 ggttatcaat aaaagcgatt gctttggtta cgccttgtaa cgcagtttta tccgcactca
     7081 tcgatccgtt aaccgcaaca atgactccat ctttggcata tatcactgta tatccatctg
     7141 gtatggtatg tccgttgaaa ttcgcagtgg ccgcaacagt actaaatgtc tggcttctac
     7201 ctgtttctga ataagctttc tcccctgctg cttttgctgc tgtgatttct ttttctgtcg
     7261 gatcaatcag ttgagtcgag gatgtggttg aggttgtctc gttgttgcta gactgattgg
     7321 tgctttctat ggactgtatt tcagaatagg gtggactatt tgtctcctct gtcgtttggt
     7381 tagcaaaatt tgacgtttct gtataaacgt ttacataaat acttgataag ctccctaaga
     7441 aagtaaaaat caagatgatc gaaatcataa tgggatgtaa gtattttctt ttagatacag
     7501 ctttgagtct agaaacaaaa tgtttttgac atatacgttg gattttattt tgagttttct
     7561 taatcatttt catccctcct ctcaataaaa aatcatcaaa taatccttta cacgttattc
     7621 taaatttaat ttttattatt ttattgatgt atagaaagtt tggggctttt tttctctttt
     7681 tattctaagg ttattttagt cttgtccttt aatacaaaga ttttattttt aaataaaaaa
     7741 tactcttttt ttacgattat tgaaaaaaaa agataaaact tgattaattg aagtattgct
     7801 taaatttgga agctatgagg gaagaataaa agagttagga cgggattatc tactattttt
     7861 ctttgtttta aatatttgtt atctttgaac ataaaagaga aatgtgtcga ctgcagtgtt
     7921 gtgtccctca tcacgaataa acggtgttca attagctgat cttgtattca cttaaaaaat
     7981 ggtaaagtac gagttgctat tccccatttt caattttcta acgctcagga caaaatactg
     8041 ttttcacaac ctcattggaa taaaagcata tatattgttt caagaacgaa atcttgttat
     8101 ctttttaaag ggcatctgtc caaaatctaa ttttaggagg tacgttttgg tatttattcg
     8161 tacaaataaa agagaattat ctttattcat gtaacgcatc caattgattt tttcatgtac
     8221 gctcctggaa cgattttact gacgattgct tatttaactg cgaatcgctc attagctttt
     8281 atcatagcta tccatatttt gaataacgtt cttggctttg tattatagat acagatgaca
     8341 aaaaaagttc aagtatgcgg tgtagaacca atatcgcata cttgaacttt tttcaatgag
     8401 tataaattaa aacttctaat tttcaattcc catcatctga tatgcccgac cgtctgaact
     8461 tcttttgaaa aagccaaatg tgattagata acgtctttgt tcgacataat cgccactaga
     8521 aaatacgttc agcagtatcg aattaacctc tgtttctgta taagtcactc catggtcaaa
     8581 gcgtttcaag acttctttca ataggatgta tttctttttt gttgaacttg gtacttgttt
     8641 tacttttcca tcattcatga atgtgtctaa aactttcatc ttttcttgct ccgtataagt
     8701 ataaaccatc ttctttctcc ttttgattct aagaatactc catttatgtt agaatattct
     8761 ggatttttta ttaaagcggt tactcttaag aaagatatta tcatagtgct ctttttttta
     8821 ctactactta taggtagatt tattctaatc cattattctc ccctaccaat aattaaagaa
     8881 aaaactggat cagggtgttc aaattacatg ctaa
//
LOCUS       DARDHA010000113         8861 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000113 DARDHA010000000
VERSION     DARDHA010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.114"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            122..2719
                     /gene="adhE"
                     /locus_tag="SDA10_002070"
                     /gene_synonym="adhC"
     CDS             122..2719
                     /gene="adhE"
                     /locus_tag="SDA10_002070"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEG4240961.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2948..4054
                     /locus_tag="SDA10_002071"
     CDS             2948..4054
                     /locus_tag="SDA10_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEG4240962.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4071..5099
                     /locus_tag="SDA10_002072"
     CDS             4071..5099
                     /locus_tag="SDA10_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEG4240963.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5105..5584
                     /locus_tag="SDA10_002073"
     CDS             5105..5584
                     /locus_tag="SDA10_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEG4240964.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5600..6895
                     /locus_tag="SDA10_002074"
     CDS             5600..6895
                     /locus_tag="SDA10_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEG4240965.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6906..7730
                     /locus_tag="SDA10_002075"
     CDS             6906..7730
                     /locus_tag="SDA10_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG4240966.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7895..8794
                     /locus_tag="SDA10_002076"
     CDS             7895..8794
                     /locus_tag="SDA10_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4240967.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
BASE COUNT         2840 a         1537 c         1968 g         2516 t
ORIGIN      
        1 tttatattta aaaataactg tgaaaaaaat cacaaaaaag tgtttacata gaaaaataaa
       61 tattgtatga tatacatgtg aattatttaa caaataacca tgacccatta ggaggataat
      121 gatggctaaa gtaatagaaa aagaaaaaac gaagacaatt gacgtacaag caatgattga
      181 tgatttggca gaaaaagcaa atgcagcatt gaaagaaatg gaaagctttg atcaagaaaa
      241 agtagaccat atcgtgcatg aaatggcaat ggcagctttg gatcaacata tgccattagc
      301 gaagatggct gtagaggaaa caggccgcgg tgtttacgaa gataaagcaa tcaaaaatat
      361 gtatgcgtcc gaatatattt ggcataatat caaacatgat aaaacagttg gtgtgatcaa
      421 tgaagatgtc caaaaagggt tgattgaaat tgctgaacca gtcggtgtgg tttgcggagt
      481 aactccaaca acgaatccga cttccacaac gattttcaaa tcaatgatcg cgttgaaaac
      541 aagaaatcca atcgtttttg ctttccatcc aagtgcccaa aaatcttctg cagaagcagc
      601 acgagtggta cgtgatgctg ctatcgctgc cggagctcct gaaaattgta tccaatggat
      661 cgaacatcct tcgatcgaag cgacttctat gctaatgaac catccaggta tcgcgatcgt
      721 cttagcgaca ggtggagctg gaatggtcaa gtctgcttac tctacaggaa aaccagcttt
      781 aggtgtaggt ccaggtaacg taccagctta tattgaaaaa acagcaaaaa ttaaacgtgc
      841 agtgaacgat ttgatcgttt cgaaaacatt tgataatgga atgatttgtg cttctgaaca
      901 agctgtgatc gtggataaag aaatctatgc agcagtaaaa gcagaattcc aagcacacca
      961 agtttatatc gtaaaaccag atgaattgca aaaattagaa gatgcagtga tgaacgaagg
     1021 aaaatatgcg gtcaatccat caatcgttgg acattctgcg atggaaatcg caaaattggc
     1081 aggaatcagt gtaccaaaag gaacgaaaat gctgattgct gaattggaag gcgtaggccc
     1141 agattatccg ctatcaagag aaaaactttc tccagttctt gccatgatca aagctaacaa
     1201 cacggaccat gcgtttgatt tgtgtgaagg tatgttgaat cttggaggat taggacatac
     1261 agcagttatc cattcagaaa acgaagagtt gcatgtcaaa ttcggtcttc gaatgaaagc
     1321 ttgtcgtatt ttagtgaata caccatctgc agaaggcggc atcggtgata tctataacga
     1381 aatgattccg tcattgactt tgggatgcgg ttcttatggt aagaactctg tttctcgtaa
     1441 cgtctctgct gtaaacttaa tcaatgtcaa aactgtagcg aaacggagaa ataacatgca
     1501 atggttcaaa ttaccgccaa aaatcttctt tgaaaagaac tctctattgt acttagagaa
     1561 aatggaaaac gttgaacgtg tcatgatcgt ttgtgaccct ggtatggtcc aattcggtta
     1621 ctgcgataca gttcgtgaag tattggcacg acgtaaaaat gatgtgaaga tcgaagtctt
     1681 ctcagatgtt gaaccgaacc catcgacaaa taccgtatac gcaggaacga aactgatggc
     1741 agacttcaag ccagatacag taatcgctct tggaggcggt tctgcaatgg acgcagctaa
     1801 gggaatgtgg atgttctatg aacacccaga cacatctttc tttggtgcaa aacaaaaatt
     1861 cttggatatc cgtaaacgga catataagat cgacaaacca gaaaaaacac aattcgtatg
     1921 tatcccgaca acatcaggga ctggttctga agtaacgcca tttgcggtca tcactgacag
     1981 tgaaacacat gtgaaatatc cgttagcaga ttatgcattg acgccagatg tcgcaatcgt
     2041 cgatcctcaa ttcgtcatgt cagttccagc atctgtcaca gcagataccg ggatggatgt
     2101 gttaacacat gcaatcgaat cctatgtttc agtcatggct tcagattata cacgtggatt
     2161 gagcctacaa gcaatcaagt tagtctttga ctatttagaa aaatcagtaa aaacacctga
     2221 tatggaatca cgtgaaaaaa tgcacaacgc ttctacaatg gctggtatgg cgtttgctaa
     2281 cgcattccta ggtatttgtc actcagtcgc acataaaatc ggcggggaat acgggatccc
     2341 tcatggacgg acaaatgcga ttcttttacc gcatattatc cgctataatg caaaagatcc
     2401 acaaaaacat gcgatgttcc ctaaatatga ctatttccgt gcagatacag attacgcaga
     2461 tatcgcaaaa ttcttaggtc tgaaaggcga aacaacagag gaattagtcg aagctttagc
     2521 aacggctgtt tatgaattag gaaaatctgt cggcatcaac atgagtctaa aagctcaagg
     2581 agtcacacaa gaaacactag atacaaccgt agaccgtatg gctgaattag cttacgaaga
     2641 ccaatgtacg acagctaatc cgaaagaacc attgatctct gaattgaaac agatcatcat
     2701 tgatgcttac aacggataag caaaagaaag cgtagaaaaa agataagaaa atagatgaaa
     2761 aagaacttgg aaaacgttgt ttctccaagt tcttttttta atttccaagt aactgaaagt
     2821 tacttttgtt atttaacatt taggtcaatt ttagacagtt tttgttcaaa aagcgatatt
     2881 tatagtggac ggtattactg ctatattaag gatataaaat gaaagcgttt aaaaaggtgg
     2941 cgtaaagatg attattaaag aagagatttt tgtacctaaa gaattagtga aagaaaaaat
     3001 tgacctgctc gttgaaaacc tgacaacgat caaagacgat aatggtgaat ttttgttgga
     3061 ctttgatgga ctgaaagtag atgacaaaag ctggacagtg tggaattggc cacaaggtgt
     3121 cggtctatat ggtatttata aaaattacca gatgacaaaa aatcctcgcg cttatcaagt
     3181 ggtcaatgaa tggtttgagg atcgtatgga agaaggcgct ccgcccaaaa acgtcaatac
     3241 aatggcaccg ctattgacta tggcttactt gtatgaagac acgaaagatt cacgctattt
     3301 gccttattta gaacaatggg cagaatgggt catgaatgac atgcctcgga caaatgaaga
     3361 cgggttacag catgccacat atggaccgga aaataagaat cagttgtggg acgatacatt
     3421 gatgatgaca gtgcttcctt tggcaaaaat cgggaaactg ttgaatcgcc cagaatattt
     3481 agaagaagct cgttaccagt ttatgatcca tatcaaatac ttgatggata aacgaacagg
     3541 attgtggtat cacggctgga cgtttgaagg aaatcataac tatgccgaag cattttgggc
     3601 tcgtggaaac tgctggttga cgattgctat tccagagatc attgaaatct tagaattatc
     3661 gaaagaagat gcattgcgca aattattgat cgaaacactt gaagcacaag tgcgtgcatt
     3721 aaaaacttat cagtctgaaa gcggtttatg gcatacgttg ttggatgatc cttcttctta
     3781 cgtagaatct tctgcaactg caggatttgc ttacggtatt ttgaaagcgg tccataaacg
     3841 ttatttacca caagaatata aagaagtcgc ttatcgtgct atccaaggat tgcttgaaca
     3901 aatcgatgaa aaaggagaag ttcaaaacgt ttcaatcggg acaggaatgg gggacagttt
     3961 agatttttat cggaatatcg gaatcacagc aatgccttat ggacagtcgt taacggtgct
     4021 ttgtttgaca gaattgctcg tctcttattg ttaaaagaaa ggaaacccat atgaaaaaaa
     4081 catcactgct gtttgcagca agttcgctta gcctaggtct gctaggtcta agcggctgcg
     4141 gaaataattc tagtgtggca actgtcgcag atgcagatgt aaatgagata gttaccttgc
     4201 gctttgctta tgcaagcaac agccagccag taattgatgc aatgaatgaa tttgggcgtt
     4261 tagtaaaaga aaaaacagat ggttccgtac agatccagta tttcccagat ggacaactgg
     4321 gtggagaacg tgaactgatc gaattgacac agtcaggagc agtagatttc acaaaagtaa
     4381 gtgcctcagc gttggaaagt ttctctaaag attattcgat tttttcagtt ccttatctat
     4441 tcaatgatga agaacatttc tttaaggtga tggataatga agaaataatg gagccaatct
     4501 accaatcgac caaaaattta ggatttaccg gattgaccta ttatgattct ggtcagcgca
     4561 gcttttatat ggtagacggt ccgatccata cgccggaaga tttgaaggga aagaagatcc
     4621 gtgtgatgca aagcgaaacc gctatcaaaa tggtagagct tttaggcggt tcgcccgtac
     4681 ctatgggaag tgatgaagtg tacacgtctc ttcaatccaa tttgatcaac ggatcagaaa
     4741 acaatgaatt tgtcttgtat acagctggac atggtggtgt ggctaaatac tattcctatg
     4801 atgagcatac acgtgtgcca gatatcatca tcatgaatga tgccatcaaa gaacagctaa
     4861 ctgatgagca ggaaaaagcc attgaagaag cagcgaaaga atcaacggtt tttgaaattg
     4921 aagcttttgc aaaagcaatt gaagaagaaa aagctttagc acaagaagaa catggagtcc
     4981 aattcaatga tgtagataat acaccgtttc gagaggcagt tgccccactt catgatatat
     5041 tcaaaaacga tcctacctac aaagaccttt atcaaaagat ccaagagcaa ggagcgtagc
     5101 tgtgatgaaa atgattttaa acaaggtatt agagatcctg ggagctgttt tgcttgttgg
     5161 gatggtcgct gttgttttat ggcaagtatt ttccagaacg gttttaggaa atccaaatac
     5221 ggtcacggaa gagcttgttc gattcggtct tgtctggttt gccatgttgt cttccgcgta
     5281 tgttgtggga caaaaagcac atttagccgt aacggtttta agtgaaaaat taactggcaa
     5341 aaaaagtaaa attttagaag ttgttgttca aagtttattt ctggtatttg ctgcgatgat
     5401 catggtgtat ggcggatgga atgctgtcac attgacaatg gggcagatct cgccttcttt
     5461 aagtattcct atgggatatg tttatctgtc agtacctgta tcgggggtac tcatcattat
     5521 ctatagcctg atcaatctaa tcgatacatt aagcaacaag ccagcaattc aagaaaaaat
     5581 ataaaaatgg aggaataaaa tggcaataga agccggatta gtgttatttt ttgtatttgt
     5641 tgtgttgctt gtattaggga tgccgattgc tataagtatt gcattctcat cgatgatgac
     5701 tttattgtta gttataccgt ttgatgtttc tgcttttagt tcagctcaaa aaatggtggg
     5761 aagtatcaat agtttttcgc tgatcgctat tccgtttttc gttctctcgg gcattatcat
     5821 gaataatgga gggatagcta agaaactagt ggattttgcg atgttgttcg tcgggcgcgt
     5881 gccgggggct ttggctcata cgaatgtgtt agggaatgct ttatttggtt ccatctctag
     5941 ctcggccatt gccgcttcta ccgcgattgg cggagtgttg atccctcagc aagtggaaga
     6001 aggatatgac cggaagtttg cgacagcggt caatatcgcg tcagctccga caggtatggt
     6061 cattcctcca agtactgctt ttatcatgta ttcacttgtt gctggtggtg cttcgatctc
     6121 tgctttgttt atgggaggtt atctcgtagg cgctttgtgg gcgctaggaa tcatgctagt
     6181 agcattcatc gttgctaaaa agaataaata ccctgttgtc gaaaaaggac agatggctca
     6241 tgctggaaaa ataataaaag aggcgattcc tagccttcta ttgattgtca tcattatcgg
     6301 tggtatcttg acggggatat ttacagcagt agaagcttca gcgatagctg tcatttactc
     6361 cctgttgatt gctatgtttt actataaaac ggtaaaacta aaagatatac ctaatatgtt
     6421 gtttcaatca gtagcaatgt cagggacgat catgttctta ttggctactt cttccatgat
     6481 gtctttcgcc atggcgttta ccggtattcc tcaggcaatt agttcgttga ttatgggcgt
     6541 aacggataat aaatttatta ttctgttatt agtcaatctt gtgttattac tggtgggtat
     6601 gtttatggat gtggcgcctg ctatcttgat ttttacacca atatttttac caatcgtcac
     6661 aagtgtaggg gttgatcctg tacattacgg gttgttttca atcatgaatc tgtgtgtagg
     6721 ctccatcacg ccgccagtag gtacaggact atatgtcggt gcaagtgtag gaggcgtaaa
     6781 agcagaggca atgctgaaac cgcttcttcc tttttatggg gttatcttag tcgtcttgtt
     6841 tttgattact tacttcccgc aaatcgtcat gtggctaccc aatatgctga gttaggagaa
     6901 tagttatgtt gaacattaca attagaggac atgatctttc cgaagtccaa acgatagaag
     6961 atcttgcaga aaaaacaaaa gaacaaggga ttcatacact gcagctcgcc ttaggtcttt
     7021 cgtttcctga aataccttct ggcgcaaatg agatcaattc tgggatggga aattatgtca
     7081 aacgagtttt agaaaagcaa gaagtgaccg taggaatttt aagttgctat atcaatatga
     7141 ttcatccaga tttaacgata cgacaagagt tgctgacaaa gttcgagcag tatcttcgct
     7201 atgcttcttc ctttggtgcg tcgatggttg cttcggaaac tggatgtgtt ttgccggaaa
     7261 tccaatatac ggaagaaaat ttcacagatg aagcatttgc tgaagctgtg tccgttatcc
     7321 gtcgcttggt aaaagctgga gaaaaatacc aaatgatggt ggggatagaa ccaggtttga
     7381 atcatccggt gtattcttta gcaagggtag agcagctgat ccaagcagta gattcggact
     7441 atctaggaat cattttagat ccgaccaatt tgatcacaag tagtgattat caagaacaag
     7501 tccaattggt agaagaagct tttgaacgtt ttggtgaaaa gatatgtgcg gttcatttga
     7561 aagattttcg tgtcgaacag gaaaaaatcg tgccggtaaa tttgggagac ggtgtgatcg
     7621 agtacacaaa aatcaaagaa atcatcatga agaatcgtcc gtacctgtat gtggttcttg
     7681 aagaaacaaa agatgatggg attcgttatg gaagaagtct tcttgaataa tgtaaagaag
     7741 acgttggtta actaatattt agaatggagg gggcggggct agaagatgcc acgtctcttc
     7801 ttttttataa gagtatataa aagcagctct ttttgattac aaaaaacgtg gtataataaa
     7861 attgtgcata agtgaacaaa aggaggtgcg gcacatgctc caaaagaaaa tcagcggtga
     7921 acaagtcaat acgatggttt ctgatcagta tgaaatctat catgtcaaag atgtcgtcag
     7981 ccaaaataaa acgatctatc atcatcatga tttctatgag atccatgcta cattaaaggg
     8041 agaagcgact ttttatttga acggacgaca atttacgatc aaagcaggaa gtgtgctgct
     8101 catccattcc aatgatttgc atcgtatcgt ccgccaatct tcagatgtat tcgagcgtgt
     8161 ctatatcttt gtcacccctt cttttctaga aagtcgttcc acgccatggt ctaatctatc
     8221 ggcttgtttt caaccaatgg gagagaagag gagtcgtgta ttgcaaatag aacaggaagt
     8281 attgaagcaa aaattatcat ttatcgacca tcctcctgat aaaaaaattt acggtgaaga
     8341 cataaaatat gaacaagctc tggtagaata cttgattttt ctcaatcaag ttgtacaaaa
     8401 agaagagagt gcgcaagaac agaatgctgt agttcctaat gagcgtgtgg aacaaatgtt
     8461 acaatatatc tcaaaaaatt tatctcatcc gctttcttta gaggaaatgg aaaagcagtt
     8521 ttttgtcagc aaataccata tcacacgaga atttaaaaag tacactggat ttacgtttca
     8581 tcagtatgta atgaagaaaa agctgatcta tgccaagcac ttgttgcgag aataccgcag
     8641 ctctagtgct gtatatagta aaagtggttt ttcttcttat ccgcattttc tgaaagcatt
     8701 caagagagag ttcggcataa cgcctaaaga atttttgaaa cgtgatacac agaaacagtt
     8761 ggttcattat gaacattttg aagatgaaca atgagaatga tcactaaaaa ataaccgcag
     8821 tagctgctgt tgtttacata gaagcagaaa ctgcggttat t
//
LOCUS       DARDHA010000114         8816 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000114 DARDHA010000000
VERSION     DARDHA010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.115"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1101
                     /locus_tag="SDA10_002077"
     CDS             <1..1101
                     /locus_tag="SDA10_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG4240968.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1609)
                     /locus_tag="SDA10_002078"
     CDS             complement(1280..1609)
                     /locus_tag="SDA10_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEG4240969.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1599..1871)
                     /locus_tag="SDA10_002079"
     CDS             complement(1599..1871)
                     /locus_tag="SDA10_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEG4240970.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="SDA10_002080"
     CDS             complement(2322..3125)
                     /locus_tag="SDA10_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG4240971.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="SDA10_002081"
     CDS             complement(3103..4227)
                     /locus_tag="SDA10_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4240972.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="SDA10_002082"
     CDS             complement(4243..5058)
                     /locus_tag="SDA10_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4240973.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="SDA10_002083"
     CDS             complement(5048..5920)
                     /locus_tag="SDA10_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4240974.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="SDA10_002084"
     CDS             complement(6005..7294)
                     /locus_tag="SDA10_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4240975.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7522..8166)
                     /locus_tag="SDA10_002085"
                     /pseudo
     CDS             complement(7522..8166)
                     /locus_tag="SDA10_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
     gene            complement(8643..>8816)
                     /locus_tag="SDA10_002086"
     CDS             complement(8643..>8816)
                     /locus_tag="SDA10_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEG4240976.1"
                     /translation="MNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQARVTSLEQLLS
                     TVWLSVRTAFFVG"
BASE COUNT         2733 a         1881 c         1393 g         2809 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacagccaga ctgtagacaa aagctgttcc
     8701 aaactcgtaa ctcgagcttg gaacagcttt ttatctactt cgtttcgaat ttttgaacaa
     8761 aaaaataaag gctccttgcc tttccatagc caggtggcta atttctttaa attcat
//
LOCUS       DARDHA010000115         8791 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000115 DARDHA010000000
VERSION     DARDHA010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.116"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(20..2863)
                     /gene="rnr"
                     /locus_tag="SDA10_002087"
                     /pseudo
     CDS             complement(20..2863)
                     /gene="rnr"
                     /locus_tag="SDA10_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(2866..3621)
                     /locus_tag="SDA10_002088"
     CDS             complement(2866..3621)
                     /locus_tag="SDA10_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG4240977.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(3724..3960)
                     /gene="secG"
                     /locus_tag="SDA10_002089"
     CDS             complement(3724..3960)
                     /gene="secG"
                     /locus_tag="SDA10_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEG4240978.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(4078..4356)
                     /locus_tag="SDA10_002090"
     CDS             complement(4078..4356)
                     /locus_tag="SDA10_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEG4240979.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(4469..5437)
                     /locus_tag="SDA10_002091"
     CDS             complement(4469..5437)
                     /locus_tag="SDA10_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG4240980.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      5652..5696
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            5720..6694
                     /locus_tag="SDA10_002092"
     CDS             5720..6694
                     /locus_tag="SDA10_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEG4240981.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            6699..7274
                     /locus_tag="SDA10_002093"
     CDS             6699..7274
                     /locus_tag="SDA10_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG4240982.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(7341..8480)
                     /locus_tag="SDA10_002094"
     CDS             complement(7341..8480)
                     /locus_tag="SDA10_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEG4240983.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         2347 a         1893 c         1574 g         2977 t
ORIGIN      
        1 gcgaaaaagg ataatgaaat tatctagaga aattttttaa tgttcgatca atttgtcgat
       61 cgatatcttt tcgtttcaaa tcttcccgtt tatcataaga ttttttccct ttcgccagac
      121 caatcaatac cttggcatag ccgttacgaa tatagacttt taaaggaaca acagtgattc
      181 cagtattttt caattcattt tcaagccgga tgatttgttt tttgtgtaat aataactttc
      241 tcgtgcgcaa cggatcatga ttaaaaatat ttccttgttc ataaggactg atatgaacat
      301 tatgcaagaa agcttctccg ttgcggacgc gaataaatcc atcttttaga ttgatccggc
      361 tgtttcgtat cgacttgatc tcggttcctt gcaagaccat ccctgcttcc atcgtgtcga
      421 tgatcgaata atcatggcga gctttcttgt tttgtgcaat taattttccc tcgccttttg
      481 gcatagctgt ttcctccaac tcattttatt ttttcttttt cttcttttta acacctttgt
      541 aaaatggctt cttccctttt ttcttaccga acgtttgctt actatcgcta tatcgtttgt
      601 tcttcttgcc atttctttct gggcgttttc ctgcttgttt tccttcgcgt tttcctgatt
      661 ttccatttcg tgtttttggc cgttccaacg ctgtaacctc ttctgcggaa acaagttcaa
      721 aatctactgc tcgttcatct ggatcagctt tgactacttt tactcgcact ttctgtccga
      781 ttttaaaagt cagaccagtt ctttcaccta caagagctaa atgattttca acaaaatgga
      841 agtagtcctg tttcaattca ttgatatgga tcaatccctc tactgtattc ggcagttcga
      901 taaacaggcc gaatttcaca acagagctga tgatcccatc gaattcttcc cccactttgt
      961 ccatcatgaa ttctgctttc ttcatggaat ctacttcacg ttctgtttct actgcacgac
     1021 gttccatctt agaactatga tctgcaattt caggaagttc cgtagcccac gaactttgat
     1081 tgctttcact tttatcttca ctgtaagaac ggatcagacg atggacgatt agatcgggat
     1141 agcgtcgaat aggagatgta aagtgtgtgt agtaatctgc cgctaagcca taatgcccaa
     1201 aattgtcttc tgaatagcgt gcttgttgca tgcttcttag caacatagta ttgataactg
     1261 cagcttctgg tttgtcttct actccttcga tcacatgctg taaatctttt ggtgtgatcg
     1321 ttcctttcgt tcctcggacc agaatgccca aagctgaagc aaaatcaaag aagcgctgca
     1381 ttttttcttc ttttggctgt tcatggattc ggtaaataaa cggcagattt aatttattat
     1441 aatgttcagc taccgtttca ttggccgcaa gcatgaaaga ttcgatcaag cgttcaccaa
     1501 ttcctctgct tcttagctgg atatcatttg ggtgtccttc agcatcaaca aggatttttg
     1561 cttcgcgatc ctcaaaagaa atcgcgccac gtttgattcg catcgtttca aggattcgat
     1621 gcaattcacc catttcttgg aacataggta cgagctgagc atagcgttgc atcgtttctt
     1681 cgtcttgatc ttctaagatt tgattgacag ctgtgtaggt catccgttct gttgtttgga
     1741 tcacacttgg gaaaatatca tgagagacta cttgtccatt tggatcaatc tccatttcgc
     1801 aactcatggt cagccttgga acatggggat tcagtgaaca gataccattc gataaacgtt
     1861 gcgggatcat cggaatcact cgatcagtca aatatacact tgtccctctt tcatatgctt
     1921 ctctgtccaa ttgactgcct tgtgtcacat agtaagaaac atctgcaatg tgtactccaa
     1981 gaaaataatt accgttatct aattttttaa ccgtaactgc atcatctaga tcttttgcat
     2041 cttctccatc aatcgtaaca atgagctgat ttcttaaatc tctacgtcca gttatatcct
     2101 tttcgctgat ttgatctgga acttcatctg cttcagacaa tacatcatca ggaaatttcg
     2161 tagggatacc attagccacg acaattgaga ggatatccat gccagggtca ttcttatgac
     2221 caatcacttg cttgattatc ccttctaaac tagtcgcata ccctttttca gggtaatggg
     2281 tcagttcgat cagcacaata cttccgtcaa ctggacgaac tccctctgct gctgcatata
     2341 ctttaaattg gttgagcttt ctatctttcg ggataacaac gccgtaaaga tccgtttctt
     2401 gtatttcttc ttctgaataa gcaacgaatt cccctgctaa ctgagtagtc gcccggtctc
     2461 gaatctcgac tactttcccc tctgctccgc gatccatatc tgtatttgct cgcttcatga
     2521 tatcgattgc tacggtgtct ccatccattg caaaaccagt cgcttctttt gggatataga
     2581 tgtcatcttc ttcgggatca atcgttacaa atccaaatcc tcgttcattt gctcgaaatg
     2641 ttccttcgat caagattggt tttaaaggaa gtttcacttt tcctttttta ttgaaaacta
     2701 tactctgttc tctttccatt tgagcaatcg tttgtactag taatttaaaa tcatcgcttt
     2761 tttggaaccc taaaccttca gcgatttctt ccatagaaaa actgcgtttg cgactgcttt
     2821 ccatgaaaaa gagaattttc tcttttatcg tttcttttgt catatttatt cctcattcca
     2881 aggtaatttt tctaaaaatt ccaagacgtc tttttctaat tcgcgtcttg ctgtaccaac
     2941 tgtaatcaca tgaccacttt caggatacca tcgaagtgtc actcgctgac ggctcaactt
     3001 gcgtgcagtt tcaaatacgc cattagggtc gatcatttca tctttcccag cttgagctaa
     3061 aaatatcggc tgttggatat ctgacaacct actttctacg attgctgcct gatcttgtat
     3121 atccattaat tgttgttcag ctaacgagcg gtaagcaact gctttttcat tgatttcttt
     3181 ctctgattta cctgctgtct tcagcacttg acgtacgtat ttttcaaagt tttcaggcac
     3241 gtttgttttt acaggagaca aaggcgaaca gaaaaaaccg ccgccaatca ctgattcttc
     3301 ttctagtgct cgtacagcaa agatgccgcc cattgataat cctaatacgg caatatcaga
     3361 aaactgctca gactgtaaaa aatggatggc ttttttagtg tctgcccacc agctttctgc
     3421 tttttcttgc aaaatatcat atggctccat tgttccgtgt cctttaaaaa gcggcgcata
     3481 aactgtataa tctgcttttt ccaaaaatcg tgcgagcatt cgaacatcgt ttggacttcc
     3541 tgagtaagcg tgaagcagca aaaccgctcg ctttccatgt tttgcataaa ggggttttgg
     3601 taattgtctc actcgtctca cctctttcgc ctcattttac catagtttct ttcataactt
     3661 cttcttaagc gttctctttg caataaaaaa agccgcccgc cgttttgcga gaggcttatt
     3721 caactacttc gaagataaat atgctaggac aaataataag atcatccaga tagctcccat
     3781 aactgcagtt gatcgctgca tgacagcttc aaagccgcga gctttttgtt tgccgaatag
     3841 ttgatccgca ccgccggtaa atgcactcgc tgcactattt tgcttgcttg gctgcatcat
     3901 aatcgcaatg accataacaa tcgataaaac gatcacgatt cccaaaatta aattatacat
     3961 atcgcagcct ccttcccaaa agatatcgtc tctttaactt tatcatagat aaaagtccgt
     4021 gacaactcta tataaatgga ataacagctg aaagttcaaa actttcagct gttattctta
     4081 tgactgacgt aagacgtctt gatattctgg tttcttttca aactcttttt ttgcataagg
     4141 gcataaagga atgatctttt tcccgtctcg tcttgctaat tctactccag tatacactag
     4201 cttttcagct aacttttgac cacgatatgc tggatctaca aatgtatggt caatgatcat
     4261 gatgtctggg cccgcatctg accatgtcat ttctccgatc tcttgttgtt catcattgaa
     4321 taaagcaaga cgattttttt cttctttgat ttccatatgc cgcactcctc aaataactga
     4381 tattttttta ttcctacctt attttagcag tctcctgatc tataaacaat tatttatcct
     4441 cgaattactt acaaaaaaga agagaaaatt aattcatcgt tattctttgt tcaataaatg
     4501 aaaaaatcac tgtccaatag gttttcggat ctttcatcga tgcttccaag tgttctgctt
     4561 ctggaacaag cagcagtgct ttatcccctg ccgtagcatt ttgaatcgtg tacgctgctt
     4621 ctgtagggac aaaatgatct ctttcaccat gaatgaccaa taaaggcaga tggttgctgc
     4681 caagctgtct cgtagctgat gcttgcttta aggagtaacc ggcgtatttc tgggcataat
     4741 gattcgcata tctcatgacc atttttttag gaaagacagt gattttactt aacatatatc
     4801 ggaattctgc ataaacagaa acatatccgc tgtctaaaat aaatcctttg accgctgatg
     4861 gaagtttttc accacttgcc atcatgatcg tagaagctcc cattgatccg ccgtgtaaaa
     4921 taatggacgc attcggctgt tcatctaaga tgagttggat ccaggcaatt aaatctagcc
     4981 gatctagcca tcccattccg atgatttctc cttcactttc tccgtgcgca cgtaaatcag
     5041 gtacaagaac gttgaatcct ttctctgcat atcttttacc tatatgagac atgtctcttt
     5101 tccctgtgct tcggtaatca tgtacgcaaa tgacccattt attctgctta tttggatgga
     5161 tgaacatctg accagctagt ttcaacccgt cataactttt gattgttttg ggtgttccct
     5221 gctcccaaaa gctttcacac tcttcttcta atttttgtcg ttttttttct actgacgtga
     5281 ctgcagcagg gtccatcaaa cgatgaccag tgtcttcata ccagtcatat cctctctgta
     5341 ctcccgctct tactaatttc tttgcataaa aataagaaag aaaatggtaa ctgatcccta
     5401 taattatcag aataagaatg aaataaaaaa taaccattgt acacccgccg ctttctgcca
     5461 aactataatc taactattat ccaatgtacc gatccatagg attctttatt ttcggcacac
     5521 gtaaattcca tttctttttc attgtatcat ttacttaaag ttctcctcaa tagatttact
     5581 tgtaagagag catttacatt cgcaataaaa gccagtatac taatttcgtg cattataatc
     5641 aagacgctcg tcttggactg gttcggaaac ttcccagaat aaaaaactaa gtatctctaa
     5701 agaaaggaga ttggaaataa tgcgaaaagt aattattgat tgcgatccag ggatcgacga
     5761 cactttagct ttatcattag ctgtcaaatc ccctgatatc gaagtagttg ctattaccgt
     5821 agtttgtggg aatgtccctg cagatatagg tacgcagaat gtgctgaaat gcctggaacg
     5881 ctgtgatcgt ttagacattc ctgtttatca aggaatggaa aaacctttga agcaaccttt
     5941 catcagtgca caagacactc atggtatgga tggtttaggg gaaacaaatt tcccgatgat
     6001 cttgagaaaa caggcagaac ctctccatgc cgttgatttt ttggctgatt atttcaaaga
     6061 aaagacaaat acatcagtca ttgctttggg tcctttgacc aatatcgctt ttgctttgaa
     6121 agtcaaccca aatattggga aacatatgga tcgttttgtc tcgatgggtg gaacttacaa
     6181 aagccatggt aactgttcgc cagttgctga atacaattac tggtgtgatc ctgaagcagc
     6241 ttcttatgta ttcgagaatc tcaaacaaac gatagaaatg gttgggttag atgtgacaag
     6301 agaaatcgtc ttgacaccga ctatcctcga atactgctgt caaatgaatc ctgaagaggg
     6361 agagtatcta aaagcgatca ctcgttttta ttttgacttc cactggaaac aagaaagaat
     6421 tttaggatgt gtcatcaatg atccgttagc tgttgcttat tttatcgagg gggccatctg
     6481 tgaaggtttc aaaagcttca ctgctgtgga aactcaagga ataagtcgtg gacaaacatt
     6541 ggttgatcgt tatgaatttt ggcagaaacc tgctaacagt aaaattatga caaaagtaga
     6601 tactcgtctt tttttccgaa agtttttaac cgttctttta aatgcacaag aagaaacgat
     6661 tatgaaagat ttggaaaggt taaagatggg ctaacaaaat gaataaaaac aaaatggcaa
     6721 ctcgcgtact tacagtgatg gctttgtgta ttgggatcaa ttatattgga ggaacgattg
     6781 ctttatggct tcgtttgcca atctatctcg attctatcgg aacgattttt gctggtgcat
     6841 tgcttgggcc tgtacctgga atgctgacag gattgtccag cggtatttta agtggagtga
     6901 ctacagatat tttttctctg tattattcgc caatccaaat tctcacaggc ttgcttgctg
     6961 gattgatttt gccaagatat ctcggcaaag ataaaaacag cttgaaactg ttattactcg
     7021 ctttgatttt gtcggtacct ggaacgatcg tttcttcgat cattaccgtt cagttatttg
     7081 gcgggatcac ctcctcagga tccagtatga tcgttcagct attacatggg ttaggtttaa
     7141 gtcagacaac aagtgtgatc ttggtacagg caggtacaga ctaccttgat cgtttgttgt
     7201 ctatcgtcgt tgtttcatta gtcatcacta tgttgccaca acgcttgata tattcaaaaa
     7261 gaaaaatagg ctgaataaac agagctggat tagcaaaata atgctaatct ggctctgttt
     7321 tttcatttat taaaaaaatc ttacgctaag taggataaaa taattccttg atatttttca
     7381 atcatatcca aataattatc gatttcaaca tactcatcta cttgatgagg caaagtaacg
     7441 acaccaggcc caaagacgac aaagtcgaag gaatggttag ctttcgtaaa ttcggctgca
     7501 tccgttgttg ctgccgctcc aactaaaggt aatggctggc tgaattgttg ctgaatgcaa
     7561 tgaatcaaag gagaatctgg atcagctttg acaggtattt tgttgtaatc gatcatcaat
     7621 tccaaatgat agtctgtctc ttgattcaat tcattgacga ttgattgcag caatgcaatg
     7681 attttatcat ttgggtattc tggaatcgat cgaatgttcc cttggagctg cgcatggctt
     7741 gggatgctgt tgacttgatt cccgccactg atcaatgtaa cattatggat tgtttttcct
     7801 aggacaggat tctcgattgt ttcagcgagg tgattcattt cagcatttgc tttcgtaatg
     7861 aattcattta ggtgattgat tgcattataa ccttggtctg gcatagagct atgcgcttct
     7921 tttccgtgcg aggtgactgt atagttgatt gatcccatgt gcgtatacat caaactgtaa
     7981 ttagttggct ctccgatgat caaggcatct aagtcatcta catagcctgc tttcgtgagc
     8041 tgttccccac ctagttcacc cacttcttct ccgacagtgg ctaataattt gactgttccg
     8101 ttaaatggtt ttcctgactc tttcagttcg atcatggcaa tcaccattgc tgccaagcca
     8161 ctcttcatat ctgtagcacc acgtccgtac aaacggttcc catggatttc tgctgcaaag
     8221 ggtgcatagg tccaagacga ttcatcacca gccgccacga catccatatg cccagatagg
     8281 cctaacactt ttcctgattg gcccttttgg taacgagcaa tcaaattatc tctaccatct
     8341 ctgtaagaaa caatctctcc tgttatgtca tgctttgcca aaagcttatt caaataggct
     8401 gctacttcac cttcgtttcc attcactgat ttaattcgga tgatctcttg taaaatcgcg
     8461 attttttctt cttttttcat ctaatcctct cccttgccat ttctttttat tgtatttgag
     8521 tgtgctcgaa ttttcaaata ggagcaacca aagtaaaaat gaagaaagag agccacttta
     8581 tgaaaaatat gctgctactt ccctcctatt ttatttatac gagaggactt gctcatagat
     8641 tcctattcta ctgttctatc cttaaaagct cttatatgat gctagttatt gttagctttc
     8701 ctttttcaag gatgatcttt tcttgtacca ttctttgtaa acatcatata cagaagctag
     8761 ttgcaaaaaa aactctctac ctgctgatat t
//
LOCUS       DARDHA010000116         8784 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000116 DARDHA010000000
VERSION     DARDHA010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.117"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            86..1147
                     /locus_tag="SDA10_002095"
     CDS             86..1147
                     /locus_tag="SDA10_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEG4240984.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1150..2046
                     /gene="murQ"
                     /locus_tag="SDA10_002096"
     CDS             1150..2046
                     /gene="murQ"
                     /locus_tag="SDA10_002096"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEG4240985.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2060..4042
                     /locus_tag="SDA10_002097"
     CDS             2060..4042
                     /locus_tag="SDA10_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4240986.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4107..4553)
                     /gene="nrdI"
                     /locus_tag="SDA10_002098"
     CDS             complement(4107..4553)
                     /gene="nrdI"
                     /locus_tag="SDA10_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEG4240987.1"
                     /translation="MNILYISISGNTRAFAKQLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4764..6188
                     /locus_tag="SDA10_002099"
     CDS             4764..6188
                     /locus_tag="SDA10_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4240988.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6369..7142)
                     /locus_tag="SDA10_002100"
     CDS             complement(6369..7142)
                     /locus_tag="SDA10_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEG4240989.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7139..8044)
                     /locus_tag="SDA10_002101"
     CDS             complement(7139..8044)
                     /locus_tag="SDA10_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4240990.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8198..8518)
                     /locus_tag="SDA10_002102"
     CDS             complement(8198..8518)
                     /locus_tag="SDA10_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEG4240991.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         2754 a         1576 c         1873 g         2581 t
ORIGIN      
        1 tttaataaaa gtattgaaat tttgtttctt tagatatata ctctagtcat agaaatgaag
       61 acggatacag aaaaagaggg ataatatgtt tggcttttca atatttatga acgattcttt
      121 gtcagaagag aagaaacagt atatccgcca aatggcatcc aatggctttg tcggtatttt
      181 cacttccatg catataccag aagatgatgt ttcggcatat caaaagcgat tgatcgaact
      241 aggaagcgaa gcgaaaaaga ataaactaga attgatggtc gatatttcag gagaggcatt
      301 ggaacgagcg ggtttttcat tagaagatct aacgcctttg aaagcaattg gtgtaacagg
      361 tttaagaatg gattatcata tttccaatca gcagattgcc gactggtcac atcaattgaa
      421 aatcagtttg aatgctagca cgatcacgcc taaagatatc gatgaattga aagaagcaga
      481 agctgatttc tcacagattg aagcttggca taattattat ccacgtcccg aaacagggct
      541 ggacaaagaa tggtatcaga aaaaaaatca atggctcaaa attcaaggtc tccttgtcca
      601 aggttttgta ccaggagata cagagttgag aggcccacta tatcaaggat tgccaacttt
      661 ggaagaacat cgagggatcc atcctcttgc aggcgctcta gatctattag caagtaacac
      721 ggatattgtt tacatcggag atgcaggtct gtcagaaaac gttcaggagc aatttgcttc
      781 tttccaaaaa gaacagacag tcttgctcca tacggaacct gtagatgaag aattttacga
      841 atatatatta gggaagcata cgaatcggca agacgatgca agagatgtca tacgaagcgt
      901 agatgcacgt tttcgagaga ttccgcctat cccagcaaga aatacagcta cgcgtatgaa
      961 agggtctatt actttagata acgaaaagta tttgcgctat atgggtgaga tccaattaac
     1021 caaatatgat ttgccagcgg atgaaaaagt gaatgtagta gctaaagtta tcaaagaaga
     1081 gctaccattg atcaaccaaa tcaaagctgg tatgaactat caatttataa gaaaagaagg
     1141 aagataaaaa tgagcaaaat caatttagag aacctaacaa cggaacgcag aaaccaagaa
     1201 acttttggat tagatgagat gagtatttct caagccgttc aactaatgaa caaagaagat
     1261 cagaaagtgg ccgctgcggt tgaaaaagaa ctgacaagta tcgaaccagt gattgctaac
     1321 acgattgagt cctttaaaaa aggtggacgt ctgatttata tgggagccgg aacaagtgga
     1381 agactaggtg tgttagatgc tgctgaatgc gtaccgacat tcggtgtaga accggaaatg
     1441 gtcataggtc taattgctgg tggagaagaa gcaatgactg tagctgtcga aggggccgaa
     1501 gattcttatg agctagggca agaagatcta atcaagctga atttgacaaa aaatgatata
     1561 gttattggta tcgcagctag cggtcgtaca ccatatgtga tcggtggatt agattacgca
     1621 aaatcgatcg gtgctgtcac aggaacgatt tcttgcaata aacaagccga aatcagtaaa
     1681 cacgcggatt tcccaatcga agtcgatgca ggtccagaat ttttgacagg ctctactcga
     1741 ttgaagtcag gaacagcaca aaaacttatc ttgaatatga tttctactat cagtatgatt
     1801 ggtataggta aagtattcaa taacttgatg gtcgatgtca aaccaacaaa tgaaaaacta
     1861 atcgaacgct caaaacgaat catcatgcaa gcgacagaag ctgattacga aacagctgaa
     1921 aagtactttg aagaagcaga tcaaaatgtt aagctggcta tcgtcatgat tttgacaaat
     1981 agtgataaag aggaagctca agcaaaatta gctgaagcaa acggatttgt aaaagggacc
     2041 ttatgagggg gaaaagacca tggcaaaaaa cgaactgact ttggaagaac tggcaaaaca
     2101 aatttataaa ggggcaggcg gcatgggcaa tgtagaaagc attgtccact gcatgactcg
     2161 tgtgcgtatg aagatcatca atgatgatct tgtaaatgtc tctgaattaa aagcgattcc
     2221 aggagtgctt ggtgtagtag aagatgaaca gcttcaagtc atcatcggac ctgggaaagt
     2281 gaacaaagta gcacagcaaa tggttgattt ggcaggcgtg aaattaggag aaaaactttc
     2341 ttctgtaagt acagctacag caaattcatc agcatctgga aaagaccgtg taaacgaacg
     2401 ggcacaagaa atgaaagcgg cacaaaaatc gaaacaaaaa ccatctaaaa tcaaatcggt
     2461 attaaaagac atttctaata tctttgtgcc aatgattcct gcatttgtcg gtactgggat
     2521 cgtggcaggg attgccgcag ttttagcaaa cttagtcaca gccggaacac ttgatgcagc
     2581 aacatggcaa caatatatcg atgtgatgaa catcttgaaa aacgggatgt ttagctacct
     2641 agttatctat acaggtatca atgctgcaca agtttttggt gcaacaccaa ctcttggtgg
     2701 agttatcggg gccgttgtga tgctgacagg gatgaatccg gaagcaccac tgaaaaactt
     2761 atttactggt gaagctcttg cagctggaca aggtgggatc ctaggtgtca tcttcgctgt
     2821 ttggctattg tcaatcgttg agaaaaaact gcataaagta gtacctgatg cggtagatat
     2881 catcgttaca ccaactcttt cattgattgc catcggtctg attgaaattt tcttcatcat
     2941 gcctttagca ggtttcattt ctgatggcat ggtcggtggg atcaactggg tacttggcgt
     3001 tggtggagca tttgccggct tcgttctagg cacattattc ttgccgatgg ttatgttcgg
     3061 actgcatcaa attttgactc caatccatgt gcaaatgatt gatgaaactg gtcgtacgct
     3121 attgcttcca attcttgcga tggctggtgc aggacaagtg ggtgcagcat tggcattatg
     3181 gattcgctgc aaaaaagata aagaattaac agaaatgatc aaaggagctt taccagtcgg
     3241 gatcctagga atcggcgaac cattgattta cggagtaaca ttacctctag gacgcccatt
     3301 tattactgct tgtatcggtg gaggtatcgg cggggcagtg atcggaatga tcggaaatgt
     3361 cggggctatc gctattggac cttcaggagc cgctttgatc ccattgatct ctgacggtaa
     3421 atggttaggc tacatccttg gattattggc agcttatgct ggtggcttcg ttgctacatt
     3481 cttcttcggt attcctaaag accgtttaga aaaagaagaa ttagcgactg aaacagccac
     3541 agtaaatgaa gtgcctgaaa aagtagtacc tcaaacttca gctgaaagtg tgactatcct
     3601 ctcatcagtt gctgacggaa cagttgtttc tttagataaa gcaagtgatc cagtttttgc
     3661 acaaaagatg atgggtgaag gctactttgt agaaccagaa aacggacata tctattctcc
     3721 agttactggg gtagtcagct ctgtcttccc aacgaaacac gctattggga tcacaacgcc
     3781 aaatggttta gaaatcttgc ttcatatggg aatcaacaca gttgacttag gcggtacgcc
     3841 atttgatgtg aaagtaacag aagggcagca agtgacacct gatacgcttg tagcagaagc
     3901 tgacttagaa gcgatcaaag cagcaggcaa agaaacatcc atgatcgtct tggtaaccaa
     3961 tatggatcgt gtaaaaaact ttgtcttgga aaaaacaggt aaagcaaaag caaaagcacc
     4021 tgtgatggat gtcaaagctt aatatgatag aaacaataga aacgaaagaa gaaactgaaa
     4081 tctatttttc agcttcttct tttttattat ttattgtttt ctgctaaaat ctgataaatt
     4141 cgtgtcacat cagatggtgt tccccgtaat tcataatctg ccaagaaagg aaagccaaat
     4201 gtttgtgcat attgtttggc tgttagacaa tactgattat tgaagttttt atttccgcta
     4261 ccgactacac caagacaaag tttatgattg tcttcatgtt ccagatattc acgcatcgtt
     4321 tcggtcaaga tttctgtatc accgttatct aaaccatttc cgccgtctaa ataagtagga
     4381 acgaaagtaa aaaatggttc tgtttctttc gtaaaatcac tattttcatg gatttctttt
     4441 aaagtaactt caggattgac aggatcttct gcatgcattt tttcagcata ttctgctagt
     4501 tgtttggcaa aggcacgcgt atttcctgaa atcgatatat ataaaatgtt catggatcat
     4561 cctccaattc tttcctttta gtatagcatc ttttttaagt tttttggtgg tgtaatttgg
     4621 aaagggacta ggcgctttgc ttgtataatg agaaaaaagc cggtagaatt atatttctat
     4681 taaagttttt attaattaag taaaaataaa aaaggtttgt tatacttttt gataaaagcg
     4741 gttttatttt ggaggatgct tacatggata cagagcaaca gaaaaaagga attggtttaa
     4801 ttccacttgc tgccttagta attggttcag cgattggagg tggcgtattt ggtattatga
     4861 cggacatctc cggatcggct ggcggcccag ctattttcag ttgggtactc gtaggaacag
     4921 caatgttgat gctctccttg tcgatcaata atatcaataa aaaacgtcca gatttaaaag
     4981 gcgggatctt tagttacgca gaagaaggtt ttggccgttt cgctggtttt atcagcggtt
     5041 ggggttactg gctcacatgc tggttaggca acgtagcttt tgcaacattg ttgatgagcg
     5101 cattaggata tttcttccca gtatttgaag gtgggcaaaa tctggcatcg atcgtgatga
     5161 gtacagtctt tttatgggga tatgcatggc tagtgaatcg gggagtcgaa aatgctagtt
     5221 tcgtcaatgc tatcgtcaca ttctgtaaat tagtgccatt atttttattt attgtagtag
     5281 ctatcagcac gttttcgatc caacaattca ttgataactt ttctagcgac attatattga
     5341 atgcgaatgg acagccgatc ccgttttcca gtcaagtatt gagttgcatc atggtgatgc
     5401 tttgggtatt tgttggtatt gaaggagcct ctgtcgtagg ttcacgagca aaaaaacaat
     5461 cagatgttgg gaaagcaacg attctaggga tattcggctt gattattatt tacgtcttgg
     5521 tttccatgct tccatatggg acaatgacga tgaatcagct gcaaacgatc aaggcgcagc
     5581 ctgctatggg ctatgtgttt gaaatgatgg ttggtaaatg gggagcagtc gtgatcaacg
     5641 gcggtttgat catttcttta gttggtgttt ggctctcatg gacgatctta ccgattgaaa
     5701 caatgggaaa catggctgaa gacggacttt tgcctaaagc atggggaaaa ctcaacaaaa
     5761 atggggcacc aacttatgct atcgtattga cgacgttatg tacgaatgtc tttttacttt
     5821 cattattatt cactgacagt gcttataact ttgcttacac acttggtaca gcagctattt
     5881 tcttcacgtg gcttttctta ggattgtacc aaatgaaact ttcttatcag cggaaagaat
     5941 gggtccaatt tttagtaggg cttttagcaa gtgcctttca agtatgggct atgctagtcg
     6001 tagcctttaa tgaagtaatg cttgttcttg ttcttttctt gccaggcatt tatttttacc
     6061 tcaaagcacg taaggagcaa aacgagcctg tacaacatat ccacttagat aagattttac
     6121 ttataggtgt gacgatggtc ggagtgattg caatggtttt atttgccact gggatgcttc
     6181 acgtatagaa gtataaaaga agtataaaga gaggttgtga gcctgtcgtt tattcaaaaa
     6241 catactagga aaggagctgt gacacaaatg ttgtcacagc tcctttttca gaataaacgg
     6301 tgtttaagag acagccaatc ggcaataagg ctgaacgtct ctttcacagc ctcttttttg
     6361 atgagtcatc atacattttt tcgcttaaat acgagatatc ctaatgctaa gaacaaaacg
     6421 atgtaagcta aattaccgaa tagcatttga ttagtagaaa gatgagtcaa ttgcttcatg
     6481 atctcttcgt ttccaatttg gttttgcaga ttcaacatac tgatcggatt ccatttgatc
     6541 cactcccatt tttggatgag tgcaataagg ataccagata tgattgacga agcaaaataa
     6601 aagacgattc ctgctgagat ggatgcgccg gaactattga taaagcaagc aatcatcaac
     6661 actaaactta aaatcagcca taaaccgata taactaccca aagtataagt gaacaagctt
     6721 tggatcagtg tttgaccagt agaaaccttc tccgtgaagg aaatcgaagg gaaaagaatc
     6781 agtttcatca acacagtgac aataatagct aaaaggtata aataaacaga ataaataact
     6841 aatgtgatcc atttgcttac taatatttct cctcgagaat actttcgata aaggagattt
     6901 ttcaatgtac catattgtgc ttccatcgaa atgatcgtgc ttgcagctgc aatcatgata
     6961 aagacaatcc aagatgtggc actaaaaagc tgagcagtca tttcaactgg atcaatgatc
     7021 gtcgtgtatt ttttggctag aagtgctgtt cctatgagca aaacaactaa tagaacagat
     7081 aaaacagcag ttgacttttt cttgatcaat ttaaataatt cttgttttac aagcaatgtc
     7141 atttttcttc ttcccccttt actgaatcca gaatttccaa tagagaagat tccaaatcat
     7201 tcgtcagatg ctggacgtct gtcacatgga tattttgtgc agacaatagc tggatcacgg
     7261 ttcctaataa ttcttcgttt tcttttaaaa gcttgatttt atcaggatct tccaaagtat
     7321 atccagcttc tgtcaagatc gttcgagcag ctagatcatt tgttgtagag ataacgatga
     7381 aatcttgcgc tgtttggttc aattcagcca ttgtacattg tttgatgatc tttccttgat
     7441 tgatcaggat cagctcatcg accatttttt caagctcgct gagtaagtga ctggagatca
     7501 agaaagaggt gccctgagta gccaagtcgg taatgatttt tcgcacatct cttgttgctt
     7561 gtggatccaa accgttcatc ggctcatcta aaataacgat agacggatgg ttcagtaagg
     7621 caagagcaat tcccaatttt tgtttcattc ccaacgagta ggatttcgct tttttgtgaa
     7681 tgtacttttc catcttgagc tgattcacga tagtgtcgat agcagataca tcgttggttt
     7741 cagagaataa cttcagatga tcatagcctg ttaagaatgg atagataccc gggtattcga
     7801 tcaatgctcc tacttgttcc aaccctttat gactagaggg agaaatctca ttttcaccaa
     7861 tacagattgt tccatctgag taatggatca gtcctaaaat agctttcatg atcgttgttt
     7921 ttccagcgcc attaggacct actaatccaa cgattttgcc aggggataag ctaaaactga
     7981 catcgctgag ggcttgtttg tgaccaaagt tcttggataa ttcttggacc gaaagacttt
     8041 tcaaaaatat tcactccttt tctatagata gtacaagcat acacctgatg agtggagaag
     8101 tcgctaggtc attggactta tttcttattt tttcataaaa aaattgaata ttaaataaaa
     8161 aggcttattt tttctgatca gtacttattc caagtgtcta gtggccgcta gtaatcttga
     8221 tgccaatgat actgatgagt agcatcccca caaagaacca agtggaaaga ttcagcttat
     8281 catgaaacaa gaccacacca attatgaccg aaccaactgc gccaatccct gtccagatag
     8341 gataagagag actcatcggc aaacttttga ttgcctttga caaaaagtaa aaactagcaa
     8401 tcatgccaat cactgtgtaa aaagaataat tgattttaga aaaaccatca ctccatttca
     8461 tcatcgttga ccagaatact tccaaaattc ctgctatgat taattccatc catgccatac
     8521 ttacattcct ccttaactat aaaaaaatac acaaaaagag gaaaatcatc ccttcaaaga
     8581 cctaatggaa tgatcatcct ctttatccta cccggtggca gattgtatat ctttttctgt
     8641 agtatagcac acctatccag atatcggcaa tctcatactt tttagtaaag tgtaccaagc
     8701 actgacggat atatcgttag cggaaaagaa gatgaatgct ttgcaatttc atagaaagaa
     8761 gttgttattt attaatcatt taaa
//
LOCUS       DARDHA010000117         8709 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000117 DARDHA010000000
VERSION     DARDHA010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.118"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(70..690)
                     /locus_tag="SDA10_002103"
     CDS             complement(70..690)
                     /locus_tag="SDA10_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEG4240992.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(766..1203)
                     /locus_tag="SDA10_002104"
     CDS             complement(766..1203)
                     /locus_tag="SDA10_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEG4240993.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            1366..2196
                     /locus_tag="SDA10_002105"
     CDS             1366..2196
                     /locus_tag="SDA10_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEG4240994.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            2180..3547
                     /locus_tag="SDA10_002106"
     CDS             2180..3547
                     /locus_tag="SDA10_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG4240995.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            3566..4375
                     /gene="yidA"
                     /locus_tag="SDA10_002107"
     CDS             3566..4375
                     /gene="yidA"
                     /locus_tag="SDA10_002107"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEG4240996.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(4418..4861)
                     /locus_tag="SDA10_002108"
     CDS             complement(4418..4861)
                     /locus_tag="SDA10_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEG4240997.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            4995..5366
                     /locus_tag="SDA10_002109"
     CDS             4995..5366
                     /locus_tag="SDA10_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG4240998.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(5535..6317)
                     /locus_tag="SDA10_002110"
     CDS             complement(5535..6317)
                     /locus_tag="SDA10_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEG4240999.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(6314..7285)
                     /locus_tag="SDA10_002111"
     CDS             complement(6314..7285)
                     /locus_tag="SDA10_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEG4241000.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            7377..>8709
                     /locus_tag="SDA10_002112"
     CDS             7377..>8709
                     /locus_tag="SDA10_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEG4241001.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNN"
BASE COUNT         2683 a         1788 c         1488 g         2750 t
ORIGIN      
        1 atcgtgtcac acctccttat tctgataggc tgatcgtctc tgtcacaacc tctcgtaaga
       61 aaataatgtt tattcttttt cagagtcaga aaattcttcc tcttcataat cttcatcatc
      121 atcgtcttcg tctatgatac ttaaatcttc ttcgatattt cctggcaagt cttcttcatc
      181 atctgtcggg tcatctgctc cgatttcttg caagtcagaa ttataagcag cgatttcttc
      241 atcttcatca tcatcgtcat caaacagatc atcgtcatct tcttctgtca gatctgtatc
      301 ttctgggtca tcgtcattgt aatcgatcgc gtcttcatca tcattgataa aggcgttgac
      361 tttcttgcgt ttacggcgac gtggagtatc ttcatcctct tcgtccattc catggtttac
      421 ttcttcatcg atcgaatcga tagcatacca tgaacgcaag ccccaacgat tatcccctaa
      481 tgagataaag ctgccgtcaa tattcaagtc agtataaaat tgtgcgagtg aatcacgaat
      541 ttcactgtct gatttcccaa gatagttttg gatctggtta gctaaatcag aaaaatccat
      601 gacatcgcca cgttgttcta agatcgcgtg tgctacttcg atcattgata attcgctttt
      661 gtttgcacct tcaaatacac taatttccaa tcaggacacg tcctttcaca gtctactcct
      721 tatcatacaa aatcagacac aaaaaatcaa acttttcttt tatatttacg ctgtaaaatc
      781 caaggtcaaa taatattcgc cgattttttc cggtcccatg aacagatcat aatcgattcg
      841 aaccttccct cctgtgggac ggtctttcaa actaacatgc agttctttcg taaaagtagc
      901 aatctggatc ataccgtatg gtgttcgata ggatgtttct aaccgttctt tatagccaaa
      961 tttcaaacgc atccgcattt ctcctgcacg gatcagctgt acatgaccat ctggaactaa
     1021 tttgatcgtt acaggaatct catcgcccgt ctcctcctga atctctttat aacgtatgta
     1081 cagcgtatcg ccaattgtca ccatttgccc tttcaaatca aaataaaaat cttgcacttc
     1141 cccttgctgt tttacttttg tacgcaattt gattgaaatc ggtgttcctt gtgataattc
     1201 cattattcat tctcctttac ttctcatagc tacattgttc ctagtttctt ttgttttgtc
     1261 aaccaacttg tccattttta cagaaaacct tttttccaaa acaactcttt gattttagag
     1321 tataatattg tagaaaatat gaatttatac aaaggagccg gttgaatgaa ccctgcgatt
     1381 cgactagatc agcacattta tcaaaaaaac gacttttccc cttttgcatt aacagatata
     1441 ttaacggaat attctaaaga acaccagcaa atctttttac atttctggca atatgactgc
     1501 acagtcatct taggtatgaa ggatgttcga actccttttc taagtgacgg gatccaaaca
     1561 ctgaaagatg caggttacgc tccagtgatc agaaattcag gtggattagg gatcgtatca
     1621 gatgaaggaa ttttgaatat ttctttgatt ttcccccaaa gtgacacaaa aaaaataacc
     1681 atagatgacg cttacgagca aatgctccta ttgactaaat acgcttttcc aaaggcaccg
     1741 atacaagcat tcgaaatcac ccattcttat tgtcctggaa cttatgattt aagcatccat
     1801 ggcaaaaaat ttgccggtat cgctcagcgt cggatcaaaa atgggatttc tgttatgatg
     1861 tacttaagtg tcaacggtaa tcaggaaaaa cgcgggcaag tcatgaagcg attttatcaa
     1921 tctgctttaa aagaagactt tggtacaaat ggctatcctg cagtggatcc tgctacaatg
     1981 gcaacacttg aagaactgct tgaccaaccc ctttttgtaa aagaagtcaa agaagcattt
     2041 gcaaaagctt ttgaaaagct gtattctgca agtgatttac aggattctac cgactggcta
     2101 aaaaaagaaa tccagctgga gcaatggaac aaccaaatcc agagaatgga acaacgtaat
     2161 aacataaagg agctacatta tgacaacccc ttataaatat caaatgctgc ctatggaaaa
     2221 agtttttcgt gatccagtcc ataactatat ccatgtccag catcaagtaa tcttagattt
     2281 gatcaattcg aaagaagtcc aacgtctacg acgcattaaa caattaggca cttcttcttt
     2341 cacttttcac ggagcagaac atagccgttt tactcattca ttaggcgttt atgagatttc
     2401 aagacgaatc tgcgatatct tttctcgaaa tttttcaaaa gaaaaaatag gcaacggagg
     2461 ctgggatgat caggaacggc tggtgacttt atgtgcggca ctgttgcatg atgtcggtca
     2521 tggtccttac tctcatacat ttgaacatat cttccatacg aatcatgaag ccatcactgt
     2581 agagatcatc acttctccag aaacagaagt gtatcagatt cttaaccgag tagaagaagg
     2641 ttttcctgaa aaggtagcta gtgtgatcca aaaaacttac cccaacccac aagttgtcca
     2701 aatgatttct agtcagatcg atgcagatcg aatggactat ttgcttcggg atgcttattt
     2761 cactggaaca gaatatggaa catttgatct tacccgaata ttacgtgtga ttcgtcctta
     2821 tgaaggcggg atcgcttttt ccatgagtgg catgcatgca gtggaagatt atatcgttag
     2881 ccgatatcaa atgtatgtgc aagtgtactt ccatcctgtt tcccgtggta tggaagtaat
     2941 tttggagcat ctgcttcatc gcgcacacga gttgtatcaa gaagatgcca gcagttttga
     3001 actccattct caattacttc tgccatttct tgacggaagc tttactttag aagaatacct
     3061 caaacttgat gatggggtct tgggcactta ctttactcaa tggtctgaat ctgctgatcc
     3121 tattttaaat gatttagcta aacgtttttt gaatcgtaaa ccactgaaat ctgctacttt
     3181 ttctggcaac cgggattcaa aattagtcca agaactcact ttgttagtag aaaaggtcgg
     3241 atataatcct gtttattaca ctgctgtgaa ttctagctac gatctacctt atgattttta
     3301 tcgcccagaa caaggcagac atcgcacaca aatcgagatt ttgcgaaatg acggaacttt
     3361 gatagaatta tcacaggtta gtcagcttgt tgctgcatta gcgggacaag aacaaggaga
     3421 tgagcgattt ttctttccaa aagaaatggt tgatccgtcc ttgcgtgatc attatgattt
     3481 atttgacgaa acttatcaag aatttgccag ccatatccgt aatggcgcat tgattgaaat
     3541 aaattaaaat atattggagg aaattatgtc tatcaaacta gtcgctatcg acatcgatgg
     3601 tactttatta aatgagaaaa gagaagtaac aaaagaagta aaagaagcga ttgccgctgc
     3661 tgttgcaaaa ggtgtttcga tcgttttatg cactggccgt cccttaccag gtgtccagga
     3721 acagttaaac gagttgaacc tttttcaaga taatgattac gttattactt ataacggggc
     3781 actcgtacaa caaacgaaat caggtaaaat catcgcccgt cacggactga cacatgaaga
     3841 tttcctagag atcgaagtga tggcacgccg tgtcggcagt catctccatt cgatcgatga
     3901 caaagcgatc tacacgccta atcgagatat cagcgcttat acgatccacg aagcaagttt
     3961 agtcaagatg ccgctaaaat atcgaacacc tgaagaaatg acaccagaca tgaacatcgt
     4021 caaaatgatg atgatcgacg aaccagaaat tttagatgca gcaattgcgc gtttaccgca
     4081 aactttccgt gataaataca caacggtcaa aagtgcgccc tattacttcg aagtactaaa
     4141 taaagaagca agtaaaggtg cggcagttgc gaatctcgca cagcaccttg gtattgatca
     4201 agatgaaatc atggctatcg gagataacga gaacgactta tccatgattg aatatgctgg
     4261 tctaggagta gcaatgggaa atgccgtttc tcttgtcaaa gaagcagcaa atgtcatcac
     4321 ttcatcaaac gacgagcacg gtgtagctga agcaatcaaa aaatatgtac tgtaataaat
     4381 aaaaaaactg agagaaagct gatctactcg ctttctttca gtttttattt ttttcgatcc
     4441 atctctcgac tgcccatcgg tggctaccac gtttcaattt cacaagcgct tcttgagggc
     4501 tcacccacca aattttattg gtcttttcag ttggttctcc tatacgcttc caagaatcag
     4561 caacaaagaa atatcctgga ttataataat aagttgcacg ataattcgag taaaaatact
     4621 catcagcttc tcccagataa gaagaaattt ctgccacaat tcccatttct tctatcagct
     4681 cacggacaat cgcttcttct ttcgtctctt ctccttcaat ttccccgcca ggaagaaaaa
     4741 aagcaccatt tggagcctcg attactgcaa tctgctgatc tctttctatg acaatatatg
     4801 ctgcataacg ggtttgataa tcagcaaatt gctttttttc accaaaaatt ggtttatcca
     4861 tgaatagttt ccaccttaac agttttattt tctatccatt ttacatgaga aatttctcat
     4921 gaacaagcac aaatcaacta tttccttact tctttttgtt atgattgagt aaaaggaaaa
     4981 ggagagatga atctatgaaa atggctcaca catgtgttcg agtgaaagat cttgaagcat
     5041 caatcgaatt ttatcaaaag gcatttggtt ttgaagaaag ccgccgccgt gattttccag
     5101 aaaacaaatt tacacttgtt tatttgacac ttcctgggga tgactatgaa ttagagttga
     5161 cttacaacta tgatcatggt gcgtatgatc taggagacgg ctatggtcat atcgctatag
     5221 cagctgatga tctagaaaaa ttacacgagc aacataaagc tgccggtctt gagattactg
     5281 acttaaaagg attacctggc actgcgcctt cttactactt tgtcattgac cctgatggtt
     5341 ataaaatcga agtcatccga ggatagttag tatggggcat tttactcttg attgaataaa
     5401 aaacaaacac caataattaa aatgagacgc aaacttttca ttaaattgaa aaagtttgcg
     5461 tcttttttta acaaccattg tagctataag gttgccactc tccaccaatt tatttttaat
     5521 tcatttgaaa gaaatcaaat aaacgaggta cctttagaaa acaaaactgc ttttcctcct
     5581 attatcacac gatcttcttt taattcacat tctagatccc cgccccttga tgacatttga
     5641 taagctgaca attttgtttt attcattctt tctgcccaat aaggaataag attagaatgt
     5701 gctgatccac atactggatc ttcattgatt gttaattttg gaaagaaggt tctagaaaca
     5761 aagtcttttt gcacactttc tgctgtcacg atgactccta ttcctaattc aaaatttttg
     5821 atagctgtaa aatctggctg caatttagcc accgtttttt catcttctaa tacgaaaaat
     5881 aaatcacgtg ctaagtaggc ttctttaatc ttagccccaa tcgcttcttc atattcagac
     5941 aatatcggca cttttttcgg cataatacta ggaaaatcca tgtaataata atctttaatt
     6001 cttgtcacaa atagattacc gctttgagta gaaaatttaa tcgtttcatc aggtattttg
     6061 taatagttaa ataaaacaaa tgctgttgct aatgtcgcgt gaccacaaag atcgatttct
     6121 cgatctggcg taaaccatct aagctcaaat tcttggtttt tcttcactgt aaatgctgtt
     6181 tcagataaat tgttttcaat ggcaattttt tgcatcgtac cttctggcag ccattcttct
     6241 agcacataaa ccgctgctgg atttcctttg aacacctcat ctgcaaatgc atcaactacg
     6301 taataatcta agttcatttt aaagcccctt tctctaattt gttataaata tattctgcac
     6361 aacaaagatc ttcaatagct agaccaactg cgtcgaaaat cgtcggcctc gattctcctt
     6421 tataaaatgt ttttttctca gaaactaatt cgcctaatgt accaacaata caagattcct
     6481 ctaaatctcc ttctgccatg gctataagca ggtccccgct ttctgttaag gcagctgagt
     6541 aatcatctac atagatttga gaagtttcca tcaacgatgt acttatctct cttgtatcag
     6601 gagtaaatgt gcccacagca ttgatatgtg cgtcttccct tacccactct ttttgaagaa
     6661 atgcctcttt actagaggtc aacgtacaaa tgatttccgc tggacgtact gcatcttcta
     6721 ctgatattgc atttataaac gttattttag gatactgttt tttagcttta gcgataaaag
     6781 tatttcgtct ttcttctaaa atatcgtaaa caaacactgt agtgatggga cgaatggtag
     6841 aaatcgcagt caaatgcgac aatgcttgtt gaccagctcc aattaaagca agtgtttctg
     6901 catgcggatt tgctaagtaa tctgttgcca gcgcagatac agcagctgtt cgaatccagg
     6961 tgactgcatt tgcgtttatc aatgctttgg gtagtccgtt ttgagcatca aacaagaaaa
     7021 tctctcctaa atgagaaggt aaatgatact gatgattatc cggaaaagcg gtaatgactt
     7081 ttgtgccaaa atatctgttc tcccccaaat aagcaggcat catcgcaaac atatttttgc
     7141 cttttccgtc aggtaaaatt tccaccaatc gaggattttg gctgatttta ccactttcat
     7201 aatcaaaaaa acattttttc attacttgaa tcgcttcttc aaaactaaaa gaagctacta
     7261 cttcctcaaa attaataatt ttcatagaca attccctcct cacaaaagat taacttctaa
     7321 aatcaataca atcaatggta aaattgtatg gtatacaatt ttaaggagtg gagaaaatgc
     7381 ctattaattc ttatgacaat tacccgttaa cttggcgacc agacaaacaa aaattgaagc
     7441 gtccttatta tcgatcgttg attcaacaat tagaacaaga tattttaaag aataaattac
     7501 agaaaaacac tcagttaccc tcccaaagag agctagctga ttatttagat ttaaatttta
     7561 ctaccgttgg caaggcttat aaatatggaa ttgagaaagg tttgctgtat acgaatattg
     7621 gaagtggcac atttatttct cccaatgtct ttgagtccat caccatctct accaatgaag
     7681 taaccgaaca tatcattgat tttggtctgg ttagttcttt tgaagaatgt aataaattcg
     7741 ttgtcccgtt tattcaatca actagtaata acccaaatct tgttggtttg cttaattacc
     7801 aagatcccaa aggcacaaat tttcaattga ctactgcagc aaattggtta aaaactcaag
     7861 gagtcattcc gtccttagaa tctctatcta ttgtttccgg tgttcaaaat ggattggcta
     7921 tcacactttc tgcacttttc tctccaggtg atagaatagc aacagaccga tatacatacg
     7981 ctaatttcat tgaattggct cagttatatc atttagagat cgttccaatt gattttgacg
     8041 agcaaggaat gcgacctgat atattgattc aggaatgtag aaaaaagaag attcatggtg
     8101 tctttcttat gccttcttgt aataatccta tagggtttca aatctctcac aaaagacgag
     8161 agcaattagc cgaagtgatt gaaaaagaag gtctatgggt tattgaagat gatatccatt
     8221 catttttaac aacttactat caacatacga tcattcctcc tttccaaaat ttattaccta
     8281 accaaacaat ctacttagct ggaatgacaa aatttatttg ttctggattg cgagttgctt
     8341 atcttgtagt tccagtacat gtccaaagca agatagaaca agcaattttt aatataaatg
     8401 tgaaaacttc tggattagat gtggaagtca tcactcaaat cctccattca aatgaagcaa
     8461 ataggatctt agaaactaaa ttaagtctta caaaaagagc caatcagctt tttgattctc
     8521 ttttcaaagt agaaaaacca acgaatcgtg tcccttacta tcgaattctt ccagttgatc
     8581 ctgagattga ccctaagata attgagaatg actttcttgc taacggtatc cgactttatc
     8641 attccagacg ctttactaca cgtgcgcaac cagatgcatt cgttcgaatt tccctatcct
     8701 ctaataacc
//
LOCUS       DARDHA010000118         8667 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000118 DARDHA010000000
VERSION     DARDHA010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.119"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..617
                     /locus_tag="SDA10_002113"
     CDS             <1..617
                     /locus_tag="SDA10_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG4241002.1"
                     /translation="DSLEKDQEKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELE
                     IYSNSYLTNKFEYRYLKNIKNEYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIF
                     DCIRVLGIILDNAIEASIESQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKG
                     ITTKKGHQGLGLSSIEEINKKNPNMFVNYQNDPYQFTTQVILLF"
     gene            632..1384
                     /locus_tag="SDA10_002114"
     CDS             632..1384
                     /locus_tag="SDA10_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4241003.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(2087..2302)
                     /locus_tag="SDA10_002115"
     CDS             complement(2087..2302)
                     /locus_tag="SDA10_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEG4241004.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(2321..2557)
                     /locus_tag="SDA10_002116"
     CDS             complement(2321..2557)
                     /locus_tag="SDA10_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEG4241005.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            2815..4968
                     /locus_tag="SDA10_002117"
     CDS             2815..4968
                     /locus_tag="SDA10_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEG4241006.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            4937..6352
                     /locus_tag="SDA10_002118"
     CDS             4937..6352
                     /locus_tag="SDA10_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEG4241007.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            6385..7419
                     /gene="sppA"
                     /locus_tag="SDA10_002119"
     CDS             6385..7419
                     /gene="sppA"
                     /locus_tag="SDA10_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEG4241008.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            7421..7942
                     /locus_tag="SDA10_002120"
     CDS             7421..7942
                     /locus_tag="SDA10_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG4241009.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            7939..8325
                     /locus_tag="SDA10_002121"
     CDS             7939..8325
                     /locus_tag="SDA10_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEG4241010.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
BASE COUNT         3097 a         1239 c         1543 g         2788 t
ORIGIN      
        1 ctgattcact ggaaaaagat caagaaaaat ttgccagatt tagacatgac tataaaaatt
       61 tattgctgag tttaaaagaa atcgctaatc aaaaccatga ttcgatattg gaaaaagaaa
      121 taaaagaatt agagatctat tctaattctt atctaaccaa taagtttgaa tatcgttatt
      181 taaaaaatat aaaaaatgaa tatttgaaaa gtttgttgat agcgaagcta taccaagcaa
      241 ataacaataa gatttgttgt aaattcgaat gtccaagtac tattattgaa gttcctgttc
      301 caatatttga ctgtattagg gtgcttggaa ttattctcga taatgcaatt gaagcatcta
      361 tagagagtca agatagagaa ctgtctctag ctatttaccg agatgataaa cagatagaaa
      421 tttcgatatc gaatagttgt agagagatac tactctctat agatactcta atgaaaaaag
      481 gaataacaac aaaaaaggga catcaagggt taggtttatc tagtatagag gaaatcaata
      541 aaaagaatcc caatatgttt gtgaactatc aaaacgatcc gtatcaattt actactcaag
      601 ttattttgtt gttttgaggg aaggaggatt tatgagttat ccaataatat tatgtgaaga
      661 tcaaattata caattaaatc agttagaacg aattattgat aatttcattc tttttcacga
      721 taaagtattt aagatagtat tgaaaacaca aagtccgtta gaagttaaaa aatacctcaa
      781 acaattccga ccaaagcaag gaatatattt cttagatatt gatttaaatc atgaagttaa
      841 cggtatagaa ttagcagaag taatcagaaa atatgatgtt caagcaaaaa tcatttttac
      901 aactactcat gatgagatgt tacccgtaac aataaaaaga agagttgaaa cgttaggatt
      961 tgtaacaaaa gatcaaacac tagatgagta tcgaaacgag attgttgagt tattgttatt
     1021 agcgcaagaa aggatagatg caacaaaaca ggttacgaat caagcatttg ttttttccat
     1081 aggttctcaa acatttactt ttgatataaa tgacatttat tttgtagaag cttcaaaatt
     1141 accacataga ttatcattgt gtactaaaga tgggcagtac gaattttatg gaagaattag
     1201 tgagcttgaa aagaaatatc ctatgttaac aagaattagt cgagcgtgtt tagtaaatat
     1261 ctttaacgtt aaagaaatag attttaaaaa acgttcacta tattttgatt cagagttggc
     1321 cagaaacttt actttaggga aagcacaaaa aattaaggag aagttgaaag aaagcactgt
     1381 ttagtggttt tttctttagg gagattgcat ttttctaata aaatagattc cttcactagt
     1441 gcaaattttt ctgaatccta ttaaagttgt accaattttt atagctgtta ttagttacgt
     1501 gcataacagc aatattagtt actggaggaa tatttctatg gtctggaacc aaaaaagggc
     1561 caaatgaata gttcttttct tgtttttatt aagtaccata aaggggaatt aaaattaaat
     1621 gtatggtgga agatgcgaaa gatttggcaa attctactaa tttttagaaa gtgtttttca
     1681 ttttttagtt aaaaaatttg tcctgtcaag tgcaatccta tgattgtact tgataggatt
     1741 tgagataatt actttcctgt ttttataaat ttaacttgca ggaacaggct ttaatgcaca
     1801 tccgacaagt acattaccaa tacctcctgt aacggccgaa ataggtcctc ctgcagcacc
     1861 aactacagcg ctgcaagtcc ttttgtaaaa atatctctac cattacattt ccaaagaatt
     1921 tttatctgac tttaataaaa gataaaaatt taatataatt ataaaaacta gcacaagaag
     1981 ctttagtaaa aagttctgtc cattccaaac ttttgaaaag ataaagaaca tacctatgtt
     2041 cgtaatcgac acaaaccaat cttgaggttt gatatttttt aattgtttac gcaccggtcc
     2101 acatgctgaa tccagcacct aataaagctc cacctacacc acaagcagct attgcataag
     2161 gtcctccggc tgaacaaagt tttactcctg ctgttgcccc agaaaaagta gtgcctaaaa
     2221 ctcccacggc actcccacct tgaatatttt tcaactcttc tgaagataat ataaatgttg
     2281 tttttgtgtt tttaatatgc atactactta ttctcctttt ttattttttt cgagcatccc
     2341 agccagctgc agcacctcca gcgacagctc catataaccc tccaacaaca ccgcaagctg
     2401 gtccgcaaat tgctgttcct aaacctgcac cacctactgc ccctgtggta agtccaccta
     2461 caacttgccc caattttccc caagcattat tgcctccacc aacagtttgt gcatttccac
     2521 aatacttagt tcctttacat tttgcatgtt taaacacgct tctttaaata atatttttta
     2581 cggtgctact gtaacatacg tatttgatta acagtaaaaa tcttcctgaa acttatcaag
     2641 tttattcttg aatagtaaaa tttccaaaat aaatttgatt caggaatgaa aaagttagtg
     2701 ttcaagaact gttttcaaag tttttgtgga attatgagac aatattttca ctgtattaaa
     2761 gctatattga gaatttttct ataaaaattt tttagaagga gttgtgttag ataagtgata
     2821 aaatcatttc cttttgtgca gcaacaagat gaaaaagatt gtggtgttgc ttgcctttcg
     2881 atgattttaa aatattttaa aactgatatc ccgattcata aattacggga cctatctggg
     2941 actgatttag agggtacttc agcatttgga ttgaaaaaga catttgaaaa attgtctttt
     3001 gattgtatgg ctattcaagc agatagtgag atctggaaag aaagagatct tatcttacca
     3061 ttaattgcac atatattggt tgataacagc tatatgcact acgttgtaat ttataaaacg
     3121 aagggaaacg tactatggat tgcagatccc gctaatggaa aaaatagaaa aacgattgaa
     3181 gcgtttcaaa aagaatggac aggtattctt ttattaccta tgcctagaga aacttatatt
     3241 cctattaaag aaaaagtagc tggattaagt tcttttttgc ctataatttg gaaacaaaaa
     3301 ggattagtat tccacatcgt tttagcatcg attttcatta cgttttttgg cataggaagc
     3361 tcatattatt ttcaaggtat tcttgatttt ttcatcccta atcaagcacg ttcaacttta
     3421 aatataattt ccattggctt gatcgctgtc tatttatttc gggtattgtt tgaatatagt
     3481 cgtagttatc ttcttattat tttaggacag cgaatgagta tggcaattat gcttcagtat
     3541 ttcaaacatg tgttaacttt accaatgaat ttttttgcta cgagaaaatc aggggaaatt
     3601 atttctcgtt ttcttgatgc cagtaagatt attgatgctt tagcaagtgc tacgttatca
     3661 atatttttag atattggtat ggtgttgctt gttggggtta cgttagctat tcagaatagt
     3721 acgttgtttt taatcactct atgctctttg cctttttatg tagttgcaat attcgctttc
     3781 gtgaaaagct atgaaaaatc gaacagggaa gaaatggcag ccggtgcagc tgtaaattct
     3841 agtatcatag aaagtcttaa aggaatagaa acaattaaag catatagcgg tgaagagaaa
     3901 gtatataacc gtatagacca tgaatttgtt aaattaatga aaaaatcatt tcgtacggtt
     3961 acgcttgata atattcaaca aggaattaaa catttgattc aattaattag cagtgcatta
     4021 attttatggc taggttctta ttttgttatg gataatagaa ttagtctagg tcaacttatc
     4081 acatacaatg ctttactagt atttttgaca gatcctttac aaaacattat taatttacaa
     4141 gtgaaaatgc aaacggctca agttgcaaat aatcgtttaa atgaaatttt cgcgattgat
     4201 tcagaaaaat cgaaccagtc ttttgaatat atgctttctg aacaattatt tcaacaagat
     4261 atcactgtaa aaaacgtttc attctcgtat agcatgaagg ccccaacgtt gaacgaaatt
     4321 tcatgtacaa ttccttcgca cagtaaaacg gctatagttg gagttagtgg ttctggaaaa
     4381 tcaactcttg caaagttatt tgttcgtttt tatgaagcat cagaaggatc gatccattat
     4441 ggaaacatta atcacttaga cattcctttt cagatattac gcgatcgagt gacatatttg
     4501 cctcaagagt catttttctt tagtggaaca atcattgaga atcttatatt cggtttagat
     4561 agcccaccaa gtttcgaaca aataatagaa gtttgtgaga tagtccaact aaatgaattt
     4621 attaatcaac agccattacg ttacgagaca ttgttggaag aaggtggaaa taatctttca
     4681 ggagggcaac gacaacgatt agccttagca cgggctttat taaaaaatcc ggacatatta
     4741 attttagatg aagcaactag tggattagat accttgttag aacatgcaat tatgaattat
     4801 ctcctcaatt taaaagaaaa aacgattatt tttattgcgc atcatttacc aatagcaaaa
     4861 gcatgtgatc aaattttagt tctccatgaa gggcatttgg tagaaaaagg aacgcacgat
     4921 gaacttcgtt ttgccgatgg tatctatcaa aaattatggg aaatctagga gagggaaaca
     4981 atttatgcag aataataatt ggttcgaaca ttctagtatc tatactaaac aacatcatag
     5041 cttctaccgt tgggtgttat accctgtaat tcttttttta tgtattattg gattgttttt
     5101 aacctttgct aaaaaagagg ttgttattcg gacaacggct caattgaacc ccgaaaaaat
     5161 agagaagtta caagtgccat tagaagctaa aataatggaa aacttcctaa aagaaaatca
     5221 atttgttcat aaggacgatg tgttggttag attggattgt tctttaatag agaatgaaaa
     5281 ggctcagata gaacaagaaa accaacgtat tactcaacaa ataaagatgg ctcagctatt
     5341 tattgaaagt ataagtaaag gaaaaaattt gttttcaacg gatgacagtt ttggctacag
     5401 taatcaatta aagagcatgt tgtcagaaaa agaatcactc cgctacgctt tgaagcaaag
     5461 tgaattaaat gatcaaaagc aattagaagt atacgaaaag acaaaaagac aactagaaaa
     5521 acaaattgag agttcagata gtaaattaca agaatggcaa caagtacagg tagcttggag
     5581 taataatcaa tcattaaaag atttttcaaa agaaatgatg gcaaactatg agaattggca
     5641 agaacaacta aataatgttt ctgatgatca aaaaaatcaa gtgaaactga caatttcagc
     5701 aagcataaat gaacaaattg agcaactaaa aaaagaagta gaacagtatc agtcagaaaa
     5761 agctaaatta gttaaaccaa ctacttctga gaatgacaga attagtcaaa cggaaaaagg
     5821 aaagcaagag ctagaacaag caataacaac tgtaaaacaa acagttacag agctaaatga
     5881 aaaacaagag aagaaccagt cagttatcaa aagtttggac gatcaacttt caaaaggaat
     5941 actaaaagct cctgtatctg gaacggttca tttaaatgaa gaaacaaagg gacaagcaga
     6001 actagccaaa ggaacagtgc ttgcagaaat ttatccgatc cacgaaaaat caaaaatgaa
     6061 atttacagca cttttacctg cgaatgaaag tacctatatt aaagaaggga tgaaggttca
     6121 ttttaaattg gatcaaaaag gtggggctcc gattacgatc gacggaactt tagatgaaat
     6181 ttcagaaaat tctactgcca ctgagaaagg tgttttttat gcagtgaaag gaacgttaaa
     6241 aacaactcaa tcgtttccgt ttcgttatgg attaactgga gagttgtctt taattgtagg
     6301 agaaaaaaca tattggcaac aaattgtcga tgtgtttcta aataaaaaat aattattttt
     6361 actaagaata taggaggagt ttatatgaat aaaagaagat ggattgctgt gggagtagcg
     6421 tgcggattat tgttactatc cattatcgtc gcgctaattc caggaaaaga taaagaggaa
     6481 gcatcaaata caaccctaac aggaataaat aagatttttt acggatctaa cgaaatcaca
     6541 gaagaaacgt tagaagaagg cgcatcaaat aaaaagattg tcaaactatc cgtcaatgga
     6601 gttattgcag atacaggtga aagcaatctt tttagtaggg aacaatataa ccatcagaat
     6661 tttttaacac agctaaagaa aattcaagaa gacaaagcgg ttaagggagt gttattagaa
     6721 gtaaattcac ctgggggagg aatatatgaa agtgctgaga ttgctaaaga aatggcaaaa
     6781 attaaaaagc tagatattcc tatctatact gcatttaaaa atacagcggc aagtggaggg
     6841 tactacattt ctgctggttc agataaaata tttgctacgg aagaaacaac aactggatca
     6901 attggtgtca ttatttctgg tttaaattat tctggcttat tagaaaaact tggcgtaact
     6961 gatgcgacgt ataaaagtgg tgcattaaaa gatatgatgt cacctcaaca caaaccttca
     7021 gaggaagaga ataaagttat acaggaattt gtgatgagtg cttatgatcg ttttgtcaat
     7081 gttgtagcaa aaggaagaaa tatggatact aatgcagtga aagaacttgc ggatggaaga
     7141 atttatgatg gtaaccaagc agtagaaaat gggttggtgg atcaaattgg ttactctgaa
     7201 gatgcattag attccctaaa aaaagagaag aaattaactg atgcgacggt tatagaatat
     7261 aaaaatgaca caactggctt tgcttcttct tggttaggta ataagattgc tgaatggcaa
     7321 gggctaaagg caactacatc agatcgtgta atttcagttt ttgaaaaact tggattttct
     7381 gatactccaa aaccaatgta ttactatgga gggctataaa atgaataaga agagtggcat
     7441 tgaaactttg cctaattatg tatttgcggg attttggtta cgattttttg cttttcttat
     7501 tgatttaatt tgtattagca tcatcaccca tgctactata ggtctctttt acaaaatcat
     7561 gggactggaa tctgacaaat ccattattac tttttatggt atagcgtcat tagctattta
     7621 tttaacctat tttgtacttt tgactaagct gaattcagga caaacaattg ggaaaatgat
     7681 ttttgggata agagtagtga gctttgagga aaaagagcta agttggcaaa ctatctttat
     7741 tcgtgagtgt atatgtaggt ttatattaaa aaattttgta tttcaaattg ggtatttggt
     7801 ggctgctttc acgcccaaga aacaacatat tggggattat ttttctaaca cgtcagtggt
     7861 tgttattcgt tcattagaag ctattaacta tggtcaaaaa atttctaatt ctaaaacact
     7921 taatctggag agaattaaat gaataagaga aaaacagagt ttattttgat tttaattagt
     7981 ggaataacag ggattttatg tagtctttta gcattgatgg gtggtatgtt tctatcttct
     8041 attgattcaa ctactttaaa tactacagaa ctaaccagta aggatataga tatgttagtc
     8101 aatctaagcg ggaatgttgt tttatttgga ggaattaccc tagttttttc gataatgatt
     8161 ttcataatag gttttttagt aagaaaaaat caaaaaacag tggtctatgg tattagttta
     8221 ctcattttat ctattattcc attttttctt ttaacattat tgtgggtaat tcctggagtt
     8281 ttaggtatta ttgcgggatt gatgcttcta ataagaaaat cttaattagg caatgtgtag
     8341 acaacgtgaa acgattaatc aattaaaagc taaatacgtt ttgagaaatg ataacttgta
     8401 gtcgatggaa gacatgtaga taaaaaaact acgtgtcttt tatttattct aattttttcg
     8461 aaaaaaagac actctctgat tgttgtatta atggaaaagg ggaaagcagc aagtaaattt
     8521 agtttttcta ttaaatattc ttcacttttt aaccttttat agtacttaga tttaatccta
     8581 tatgtttaga gagttcattg gcagcatgat tttgcaaagc ttctcgaatt attgttatat
     8641 tagaaacttt actgttcata attatgc
//
LOCUS       DARDHA010000119         8635 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000119 DARDHA010000000
VERSION     DARDHA010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.120"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(113..196)
                     /locus_tag="SDA10_002122"
     tRNA            complement(113..196)
                     /locus_tag="SDA10_002122"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(160..162),aa:Leu,seq:cag)
     gene            724..1224
                     /locus_tag="SDA10_002123"
     CDS             724..1224
                     /locus_tag="SDA10_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4241011.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(1414..1758)
                     /locus_tag="SDA10_002124"
     CDS             complement(1414..1758)
                     /locus_tag="SDA10_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEG4241012.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(1912..2832)
                     /locus_tag="SDA10_002125"
     CDS             complement(1912..2832)
                     /locus_tag="SDA10_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG4241013.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3019..3510
                     /locus_tag="SDA10_002126"
     CDS             3019..3510
                     /locus_tag="SDA10_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEG4241014.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(3534..5909)
                     /locus_tag="SDA10_002127"
     CDS             complement(3534..5909)
                     /locus_tag="SDA10_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEG4241015.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6235..6861
                     /locus_tag="SDA10_002128"
     CDS             6235..6861
                     /locus_tag="SDA10_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG4241016.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            6884..7588
                     /locus_tag="SDA10_002129"
     CDS             6884..7588
                     /locus_tag="SDA10_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG4241017.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            7780..8613
                     /locus_tag="SDA10_002130"
     CDS             7780..8613
                     /locus_tag="SDA10_002130"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEG4241018.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2621 a         1647 c         1535 g         2832 t
ORIGIN      
        1 ttatttgaat ctggttccgt tgtttaaaat ttttcctata ttcttttaga taatatgtta
       61 ttttctttct catcgctcca ggtgtacctt ctctttataa aaaaacaaat tatgcggcta
      121 agaagactcg aacttccacg aggtcaccct cactagcgcc tgaagctagc gcgtctgcca
      181 attccgccat agccgcatca catcttttag tttaggatta catgtgtttt ttgtcaagaa
      241 catatttttg ttctttttca tttctgtacg ccctccctta tgccatctac acaaataatt
      301 catccgagca actccatcat ctattgaggg ttggtgaaaa aatctgtttt atccatatga
      361 tcgatttcaa tgttaaacga acgactctgg gattagtaac tattttattt ttttcttcat
      421 ttcttttttc ctctttttct cgattcagct atcttctttc atttggatat tgattatttc
      481 ttctgttgat tactatcctt ctggaattct agacgctgaa gtaattcacg ttttctttct
      541 tctagagatc ataagtaacg aatgaacgaa cttcaaataa tttttttatt gtaattcata
      601 tttgtattgt tcgatgattc ttaatacgat atgacacaat aaataaaaat aggcatggct
      661 atttacaaca atagtgctat agtagaaaac aaatgaataa ggttacttaa agaggtgaat
      721 acgatgaaac tgatcaaact atgggaagca gatttaaaga aagcttatca gctacaaact
      781 agctttggac caaatgagag tggatttatc aacaatgctt atggctatac atttgctgaa
      841 tttttagatt atgtaaaaaa atgcgaaaaa acgtctttag gaatcgatct tcctgagagt
      901 tacgttcccg caaccgtctt tattttagaa agtgatgaac atgagtatgt aggaattttt
      961 aatttgcgcc ataaactgaa cgaaacatta agaaacggcg ctggccatat tggttacggc
     1021 atctccccta attatcgtaa aaaaggatat gccacacaag gattagcatt agtactacaa
     1081 gaggcaaaaa aaatcggcat tgatgaggca cttttgtctg taaataaaaa aaatcttggc
     1141 agtcttaaag cacaacaaaa aaatggggct gcgatttatc gtgaagatga gaaagaatac
     1201 tatacgaaaa tcaaactgat gtaaaaaaga aggattccaa aaggaaaggg atttgaatgt
     1261 cctttatcaa tagaacacat ttatttaatt aacttataat atagcagaca aaattttata
     1321 aacaaagaaa ccttttatct cttttcttct agaaaggata aaaggctttt ttgtttttcc
     1381 tatttgcttc tcatagctag tcagccaaga tttctatcgt atccccaacg caatccgtgc
     1441 ataacggctc atcttatctg tcgtccatgc tggataccag actagtttga cttctgcttt
     1501 cgttacttct ggcacttctt ttaatgcgtc atggatagat tctgttaaaa tatctgctaa
     1561 tggacagccc atagttgtta gagtcatctt aatgactgtt tctccatttt caggattgaa
     1621 ttctatatca taaatcaagc ctaaattgac gatgtcgatt cctaattcag gatcaatcac
     1681 catttccaag gcagcaagaa tacgttcttt gattagttct atttcttgtc cagaccgttc
     1741 ttggtttgct tcgctcatcg tacgacactc cttaacacga tttttattta tcatactctt
     1801 tttttagctg cttgtaaatg agaatttaga agtaagattc tcgtttaaat ttataaaaat
     1861 gttcttatta gatgtaatag attaaattta ttctaaaata aacctgattc ctcatttttt
     1921 ttgtgaacga ttttttattt ctggcaatac gccagttgtt cgatacattt tttgttcgtt
     1981 ttcatttgca aaccggccaa atactgaatg agttggtaaa atattgataa atgcatcgtc
     2041 gtcgatttca tgaacgatct gatcaagatc atacatttcg tagcgggtga tgaccatcat
     2101 gatcatgttc ccttgttttt ttgaatagcc accgacagac ggcagtaatg tcattccacg
     2161 taccatccgt tttgctagtt cttctgttac ctcttctggc ttgacggtaa cgatcattgc
     2221 tgttactttt tgatgactcg tgtggatggc atcaattact tgagtcattg cataaatgga
     2281 aataatcgta tacaaggcac tttcccagtt aaagaagaag cctgcgatga ctacaatgat
     2341 accatttaga atcagcatgt agtttccgac tgtttttcca gttgtttgtg aaagtacaag
     2401 agagatgata tccatcccac cagttgtaaa gcccattttt aaagataatc ctacgccaag
     2461 acctaataaa acaccgccta ctagggcatt catcaaaata ttatcagtga tctgtccgac
     2521 tggaagcagc atcgtcatca ccgatacaca tacaacattc acaaaactaa ggatcgttgc
     2581 tcgttttcct actttgagaa accctaatac aaacacaggg atattcaaaa gaaaaataaa
     2641 tgttcctgtg tctatcttga tatgaaacca ttcatataaa agcgaggaaa tgatttgagc
     2701 gatcccattc atccctgcag aaaagacatt ggctggaatc aaaaaataat ttaaagcgac
     2761 cgcagcggtc aatccggtta tcaaaataac cactattttc tttaatcttt catttttatg
     2821 atacgcagtc ataattttac tcacaagcac ctctccccta ctttggtatt ccaaatgtaa
     2881 gaataacagt ttatctatta aaataaaata cttcgaactc aaatgagaag aattttagca
     2941 aacaggtaag aatctgccct aatcctatga taagatagat gtatggtaaa tatttatatg
     3001 tatatcagga gggttcctat ggcaaaagat gctagcttcg acattgtatc agaagtcaat
     3061 acagaagaag taaaaaatgc gattcagcaa acaacaaaag aattgtccaa tcgctttgac
     3121 ttcaaggatt ctacagtaga aatcaaatta gagaacagca atctagttgt attgagcgat
     3181 gatgatttca aaatcgaaca gatcaaagat gtattatttg gtaagctgat caaaagaaat
     3241 gtgccaatca aaaatatcca tttttccgaa tcagaacacg ctctaggtgg caaagctcga
     3301 caaactgccg atttggtcaa cggaatcgat cgtgaaaacg cgaagaaaat cactacagct
     3361 atcaaaaatg aaaaactgaa agtaaaagca caaatccaag aagatcagat tagagtgaca
     3421 ggtaagaacc gtgatgatct acaagctgtg attgctttat tacgtaaact tgacttacca
     3481 atcgagttgc aattcactaa ctatcgttaa tcaaaaaacg aggctgacgt ctgtcattcc
     3541 tcgttttttt gatttactgc ttcttttgat aaccaaaaac gctgtattcc ttcttccaat
     3601 tccccaacac ttcgtacttc tcttgctgtt tgccaatgtt gtgggaataa acgtcgataa
     3661 gaaaaactag aaaatcgttg atctgccaat acaacgattc cttgatccgc catgccacga
     3721 atcactcgac caccagcttg caagactttg ttcattccag gaagctgata agcgtaagca
     3781 aatccaaggt gttccttctc atcataatat gatttgatca gttcctgctc atgattcatc
     3841 tgcggcaaac ctacacctac gatcatactg ccgatcaatc ggtcccctct caaatcgatt
     3901 ccttctgaaa aaatcccgcc aagtacgcaa aatccaatca atgtttcagc gggatttgtt
     3961 tgaaattttg ttaaaaatcg ttcgcgttct tcctcattca tgtccgtttc ttggattagc
     4021 acattgctgt cgggatattg ctgtctgaat gcttccaccg caagatctaa atattgataa
     4081 gaaggaaaga aaacgaaata attcccgacc tttccatcag caaaagcatg aatcgtctct
     4141 actaattttg ccagactgtg ttcccttttt cgataagtcg tctctaggta atttgcgatg
     4201 aaaacttgct ggtttccttc tgggaaagga ctcggcagtt tgtaagccag acttttttgt
     4261 ccccccagtg tttcctgata ataagagagt ggtgaaaaac tagcagaaaa aagaatactg
     4321 ctttttcctt tatccaacga ctgctcaaga aacaagcttg gatcaataca aaactctttt
     4381 actatcagat cgcgatacgt tttttcgact gttgtttcgt agtgatcatc ataaaattca
     4441 ctgaccttca aaaaatgaag caaatcaaaa taataaggaa gcaattgttc ttgtgatggg
     4501 tgctcaggaa attctgccag ccattcttta attttttcgc ttaattgata acctgccttt
     4561 actaaagatt cagccggtgc tttttgatga tgataatccc aatgatcttc ttttgctatt
     4621 tctcggatcg aatcaaattc ttttaatagt ttgttgaaac ggcgatgaag ttttttgaat
     4681 tcctttggta tagcttcctt acttcgtttc gttttttcat aagatagttc tgccgaatac
     4741 atctctcggg aacgattcac caaattatgt gcttcgtcaa tcaaaaacag atagtcctcg
     4801 tttttttcat cttcaaaaaa acgtcttaaa taaacagttg gatcaaataa ataattgtaa
     4861 tccccaatga ttacgtcaca ccagatactg acatctaatg atagttcgaa aggacaaagt
     4921 gtatgctttc ttgcataagt ttcgatgact tcacgagtga tctgattctc atgattcaaa
     4981 agatcccata aaccttcatt aatccgattg taataaccat tggcatatgg gcactgatct
     5041 ggattacagg tcgtctcatc taaaaagcag atcttgtctt tcgccgtcaa agtgacactt
     5101 tttacttcag ctcctgtatc ttttaatgct gtcatagcat cttcagctac ttgtctagtt
     5161 attgttttag ccgttagata aaagatcttt tctccctctt cttcccctac agctttcaaa
     5221 gcggggaata acgtagagat tgtttttcct gttccggtag gtgcttctac aaacaatttc
     5281 tgtttcgtac ggatcgtttt ataagcagct accgctagtt cacgctgacc tttcctatac
     5341 tcatcatggg ggaatttcaa agccatcaaa gaggcattgc gtacagtgcg ccagttttct
     5401 tgaaaaacaa gccattcttt ataatcttca gtcagctttt taaaaaaagt ttctaactgc
     5461 tctcttgtct gatgttcaac ctttctcgtg atcacttctt ctgccgtctg gaagtaagtc
     5521 aattgcagat ttatttcttg taaatcatgt tcttgactat agatataggc atacaccctt
     5581 gcttgatgaa agaaaagttc cacttgttct ggttctaaat cttcaaatcg tggttcggag
     5641 gttttgattt catctatata ataaataccg tctcttttga aaataccgtc tgcacggcct
     5701 tcaacgatca ggtaatattc atctaattga actgttgttt ttaaaaagac ttctttttca
     5761 tagttttctc ccgcatcttt ttggagcttg cggtggattc tcgctccttc taatgcagta
     5821 tggttacttt ttttacgatt gtcgatactt ccacgtcgca aaataaattc tactaagtgc
     5881 cgcacagata tttttgactg tttgatcatt tccaccacct ctttctctct aagattacct
     5941 gaaaaaacct agtttagcaa aaagagctct ctttccaaac ataaaaaaac accctgctga
     6001 ttaggcaggg atataggata tagaatttgg atttttggat gaatctaatg taacaaggac
     6061 atattaaaat gctatgaaaa cttactttcc ttctctttag aatatccaaa tacttttcat
     6121 atagtctgat aattcagata agtaaagaga aataagtacc tgtttttgtt tcctagcaat
     6181 gaatgcgtta tactaatgga tgaaagaagt tttggctatc aaggaggaat gcacatgcat
     6241 atcgaaagat taaaaacagg aatgattgaa gaaaactgtt atttggtcta caacgaagaa
     6301 gcacttttga tcattgaccc tggagaagat gcggaacgaa tcaaacaaga aatcaaaaaa
     6361 acaaaccaac agccggtagc gattttgctt acccatactc actacgatca tatcggtgca
     6421 gtggaacctt tacgcgcgta ttatcaaatc ccagtttatg tcagcccact agagcaaaaa
     6481 tggctaggtg atccgatttt gaatctttct ggactcggta gacatgatga tattgctgat
     6541 gttattgtct cgcctgcaga atatgaattt gaaatgaaaa cttatacttt aggaaacatg
     6601 tcttttcgcg tcgtccctac cccagggcat tctataggaa gcgtcagctt tatttttgat
     6661 gattttgtcg tttctggcga cgccctgttt agaggaagta tcggtagaac cgatctgtat
     6721 acagggaatt tagaacaatt acttcatagt attcggaccc agctgtttgt attacctgat
     6781 gaatttgcag tctatccagg acatggcgat gcaacgacga ttgaacagga aaaaaggaca
     6841 aatccttttt tcaatcgtta aaacgactta ggaggagaat aaaatgacag aaactacact
     6901 ttatatcgta cgacacggaa aaacgatgtt caatacaatt gaacgagtac aaggctggtg
     6961 tgatacaccc ctcacaaagc aaggacaaga aggcattcat tatttaggtc gcgggttaaa
     7021 aaatgtcgac ttttcatttg cttattctag tgacagtggt cgtgcaatcg aaactgcccg
     7081 tattattcta ggagaacatt ctaagggaaa agaaattcct tattttattg aaaaaaggat
     7141 tagagaatgg tgtttcgggt ctcttgaagg tggatatgac atggaaatgt ggggtgtgat
     7201 ccctcgtgta ttgaatttta aaacatatga tgaaatgttt accacagaag tttcttttga
     7261 acaaattgct aatgctgtat acaacttaga taccgctggg tgggcggaaa cttatgaaac
     7321 attgcgagac cgtgtatgga caggatttga agatatcgcc catcactgcg aaaaaaatgg
     7381 tggtggaaat gttttagtcg tatctcatgg tttgacgatc gcctttttgc ttaatctgat
     7441 cgatcctaaa cagcctgttc gtgctgggct tttaaatggc agcgtaacaa aggtcactta
     7501 tgcaaacggt cgattttcta tccacggcat caatgatacg tcttatatcg ataacgggaa
     7561 aacacatagt cgcgactcgt ttctataatg gggatatttt aatttttacc agagaatcat
     7621 aaaaggcaaa gtctgaacga gggctttgtc ttcttttttc tcttcgtgta cttatctgct
     7681 ttatttctca agtttttctt aatattaaaa aacagattgg gtttctcaaa aaataacggt
     7741 atactataag atgtatctta attgggaagg aaggaattta tgttttttgc aaatattatc
     7801 aaagcagtga ttctcggtat tattgaagga ataacggaat ggctgcccat cagtagtact
     7861 ggtcatttga tcctagcaga tgaattcatc aaactggatg ccagttcaca atttatgtcg
     7921 atgttcaatg tcgttatcca attaggggcg atcttggctg tcgtcgttct ttattttcac
     7981 aagttaaacc cattttctcc aagtaaatca acggtagaaa aaaaagatac gtggacactt
     8041 tggtttaaag tgatcgttgc ttgcatacca gccatcatca ttggtttgcc tttggatgat
     8101 tggttagaag caaatttcca caagttctta ccagttgcca ttgttttgat cgtttacggt
     8161 attgccttta ttattgtcga aaaacgcaac aaaaacaaaa cacctcgttg gacttctctg
     8221 aatgacttta cgtaccaagc agctcttctt gttgggttat tccaagtctt ggcacttgtt
     8281 cccggaacat cccgttctgg cgcaacgatc ctaggatcta tcttgattgg tgcttcgcgt
     8341 tttgtcgcta cagagttttc atttttctta gggattcctg tcatgtttgg cgcaagcggc
     8401 ttgaagatcg tcaaatattt agctgaaggg aattcattca aaatggaaga aactgttgtt
     8461 cttcttgtag gaacattcgt atcttttgtg gtttccgtct tagctatcaa attcttacta
     8521 agctatttga agaaaaacga ctttaccgct tttggctggt atcgtattat ccttggtatc
     8581 attttgattg gttattggtt tattgcaatg tagaaaaaac cagaacactt ctctt
//
LOCUS       DARDHA010000120         8572 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000120 DARDHA010000000
VERSION     DARDHA010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.121"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..776
                     /gene="menA"
                     /locus_tag="SDA10_002131"
     CDS             <1..776
                     /gene="menA"
                     /locus_tag="SDA10_002131"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEG4241019.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1590..2936)
                     /gene="gor"
                     /locus_tag="SDA10_002132"
     CDS             complement(1590..2936)
                     /gene="gor"
                     /locus_tag="SDA10_002132"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEG4241020.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3070..4323)
                     /locus_tag="SDA10_002133"
     CDS             complement(3070..4323)
                     /locus_tag="SDA10_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEG4241021.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4329..5252)
                     /locus_tag="SDA10_002134"
     CDS             complement(4329..5252)
                     /locus_tag="SDA10_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEG4241022.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5473..7965)
                     /locus_tag="SDA10_002135"
     CDS             complement(5473..7965)
                     /locus_tag="SDA10_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEG4241023.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7972..8439)
                     /locus_tag="SDA10_002136"
     CDS             complement(7972..8439)
                     /locus_tag="SDA10_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEG4241024.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2354 a         1814 c         1422 g         2982 t
ORIGIN      
        1 attacatgga ttatgaaaag gctcattctg aagtatataa gtatgaagaa aatgtcattg
       61 gccgcagaaa tctatctcct gtattgattc gaaacatgat tttagctatg attgcatttg
      121 tttttgttat cggcgttatt ctaacgatca atacgggttg gcttttactt ttgatgggat
      181 ttatctgttg ttttattgga gtattctata cattcggacc gattccttta tctcgaatgc
      241 ctttagggga aattttcagt ggggtgacta tgggccttgg aatttttgcc atggtcgttt
      301 atgtcaatac attcggttca gatgtctatg atctgacagt caacttttca tctggacaat
      361 ttgatttggt aggaaatctt tgggctatag cagcaatcgt attagcttcc ttacccttgg
      421 tgtttacgat tgccaatatc atgttggcta ataatttgcg ggatcttgac cgtgatattg
      481 aaaatcatcg ctatacacta gttttttata tcggacgtcc tgcgggaaca gtcttattcc
      541 agctattgat gtatgcttgc tatttcgtga ttttaatggg gttgttgagc ggtatctatg
      601 aatggccgat cttggtcacc ttcttgacct tgccaattgt gaagaagcat ttggatcaat
      661 tcaagcaaac gttaccccag cctatcagtt tcagctattc catcaaaaat atggtcttat
      721 tcaatgccag ttatgtattg gggctattac taacaatcat tatgcaagcc atttaacagc
      781 atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata agaacagcca aataaaaata
      841 gctccttata tttttcgttt ggttgggctt attaccgcag atgcaagctt ttgaacaccg
      901 tttatcagga ggttgtgaaa aagtcgctta gtttcgagta ttaagaaaaa ttttgtgaaa
      961 atggctttcc acatttttgc ataaatttgg cttaataccg agaaactggc ttttgaacac
     1021 cgtttatcag aggttgtaaa atcagtgttt tgtcctgagt attagagaaa caaatggaaa
     1081 aatagttcct catattttgc cgttttttga gctaataccg aaggacagct gattgaacac
     1141 tatttgttcg gaaaaagtga ctaactatga cacaattttt ttcacagtcc cttttttcac
     1201 ttgatttgat tcgtaccagc gaacttgacg agaactttct tggtttggta actatatggt
     1261 attcttttaa catcctcgtt tgcttcttct aatttattta tacaaagtca cactttttta
     1321 aacatctgtt cttcgataca tttctatttc ttttaaacat cgataccaat attttgaact
     1381 taagagtgca taccaaatgg attcatttac tcttcgtcag ttacgaagat aatcaaaatc
     1441 tccaacttcc ctacttattt cgttttgaga attatcgaag caatcttaca taaataacta
     1501 aaaaacaaaa aaccagaaaa tcagtatact gattttctgg ttttacatgg tttgttcaat
     1561 aaaaagctgt tggaactgtt aatattaaat tatcgcatcg ttacgaattc ttccgctcct
     1621 gttggatgga tggctactgt attgtcaaag tcttctttag ttgcacccat cttgatagcc
     1681 acggcaaaac cttgcagcat ctcatcgaca ccaacaccaa tagcatgtaa gccgacgatt
     1741 ctttcttctt ttccgacaca gatcaatttc atttcgcatt tttggcgata atcatttaat
     1801 gcaaagtaca ttggggtaaa gcgtgaacga taaactttca attcattttc gccatatttt
     1861 tccaatgctt cttcttctgt caaaccaatt gttgcaattg gtggatgcgt aaagacgact
     1921 gtcggaacaa gtttatagtc aagataagaa tctttctttc cattaaataa acgttctgat
     1981 aaacgtctac cagctgcgat tgcaacagga gtcagatcta atttaccgat cacatcacca
     2041 acagcaaaaa tatgttcatc cgttgtattt tggtatttat ctactttgat aaaccctttt
     2101 ttgtccaatt ccacatttgt attttccaga cctaattttt cggtattcgg tacacgtcca
     2161 ccggcaaaaa gtacacaatc accagttaag gtttcaccat tttcaaatag caccgtatat
     2221 tcgtcattgt ttttagtaat ttctttagca atatgatgtg cgtaagtttt tattccagct
     2281 tcttggtaca tttggatcaa attgtccgaa agcattttat caaacgttct aagtggtcgt
     2341 tcttttcgat atgcccacat gacttcacta cctaaaccac gaaatacacc tgaaagttca
     2401 gccgcaatgt atccagcacc taaaacgatt gctctcttag gtaactctgt taattcaaaa
     2461 aatccatttg aatcaattgc gtattctcca ccaggaatgg tcattttact tgcacggcct
     2521 ccagtagcga tcaagatttt aggagcacga taggtttcac cgtttacttc gatcgtttga
     2581 ctatcgataa aagtagcgta accttttata gcctcgacat gattgctatc taatcctctt
     2641 tgataagcac cgtgtagaaa atcaatatat ttttctcgtc tttctactag accggcgaaa
     2701 tcaacatttt ttaaatctgc ttggataccg taacttgagg catctcgttc aattgtttcc
     2761 aaaatcgtac ttgcttgcca cataactttt ttaggtacac aaccgacgtt gacacatgtt
     2821 ccgcccaatt cattcgcttc aatcagcaaa acttttgcgc catgcatccc tgctctgttt
     2881 gctgaagcaa taccgccaga gccgccacca attacgatgt aatcatatat ttgcattttg
     2941 ttcctcctta ttgaagagat caatcaaaac ctttatttaa cgatcctttt tctacagaaa
     3001 acaagtgtaa cacttttctt gaattttgta tgcgaagatg cttaaaacag ggatatatca
     3061 gctttttcct tatttacgtt ttcggatcat atctccaggt ttcaccggct gtgcgactgg
     3121 catagttaaa atcatcattg gatttggtgc tcggtcaatg gattcgcctt cttcattatg
     3181 cataacttca acggtttgac tgaaatgact gaatcctgga ccataaaatt cgatttcatc
     3241 tcctacagaa aacaagtttc tctgacgtat cgtagcaatc tgtgttgcct catcataaga
     3301 taatacttca ccgacaaatt tatatactgg gattttacga cgttcaccga ataattgttc
     3361 gttttcagtt ggtgtattgt agtaaaaacc tgtagctaat tctcgttgtg ctactttcca
     3421 taactcgtcg atccattctt gcttgcagac atagttttca ggatcttcca tataactgtc
     3481 tacagccgct ttatagacat ttgacaccgt agatacataa tggatcgatt tcattcttcc
     3541 ttcgatcttg aagctgtcta cgccgttttc aatcaattct ggaatatgtt cgatcattga
     3601 catatccact gcactcatcg agaattcttc ttccacttcg cctgtatcag ttaaactgtt
     3661 tcgttcttga ccaaaaggca tatcgaacaa tccatacttc cagcgacaag attgagaaca
     3721 tccgccacgg tttgcatccc gcatcgacat atggttggac aatgtgcatc gaccagaata
     3781 agagatacac atggcaccat ggataaatgc ttcgatttcg gtatccgtat ttttacgaat
     3841 ctctgccact tcctccattg aaacttcacg agctaaaacg acacgttcca acccttcttc
     3901 tttccagaat tccaatgtct caaaattagt tgctgaagct tgagtggata aatggatcgg
     3961 cagacctggg gcttctgtcg cacaaatttc gatcaaagct ggatcagaaa caatcactgc
     4021 agaaataccg acatcacgca aactgcggaa aaattcccct gctccatctt catttccttc
     4081 atgggtgacc atattagcag caacgtatac cttcgcatca tgttcttttg caaaagcaac
     4141 tccttcagcc atttcttcat aagtaaaatt acctgcacgg cttcttaaac catatgcatt
     4201 cccgccaata tatactgcat ctgctccata atggatcgcg gtttttaact tttccagtgt
     4261 tcctgctggc gctaggattt ctggtcgttt caattttctt tttactgtca tttctttcat
     4321 catactcctt atttgatagc agaaggatca atataaaaga atcctgtatc taattcacgg
     4381 ttttcaggat gcaatttact gatttcgcta gtcatttttt ccgctttttc ttgatcccag
     4441 ctaccttcta aaatttgttt ttttgcttcg gcgaatagtc gagtgatgtt tacgaatttt
     4501 tcgcctggag taaataatcc gtccaatttc catgtacagt aatgatgctg ataaagttca
     4561 ttcaattcat tcgagagatt cagatcattg ctggcaaaaa tatgtgtgcc atgactatct
     4621 tcataaatag aataatgtgt ctcttctttt ttaggttccg agataaaaag acctctttca
     4681 cgatcttttt gttcgtcttg tttcgtatag ttatagtaat tctgtagcag tggacgtttg
     4741 gattgatgga tgcatgtagc accatacaca agggtttcta caggaatatc gagtttttct
     4801 tccatcgcct tcatctcttc aaaaggaact tcacgagcaa gtactgctcc aactgcgcct
     4861 tttttcgccc agaaattgat ttgacgtgcg ctcgttacta atgtttcagc atcataaata
     4921 tacggtatac gcaattcagg attttttttc atcgtataaa taatacctgt atcaccaact
     4981 gtgatcacat ctacgtttat ttcttttaaa aaagccaaat attcaggtac atttttcatt
     5041 ttttcaggat gaatgatccc atttacagca atattcgctt ttttaccata cttgtgtgct
     5101 aattctacca attgtcgctg ttcttctcgt gtaaatgaag caggcagacg caatccaaat
     5161 tgctcttcac cgaaatatag tgtatctgca cctgcagcaa gcaactgttc tgcttgttca
     5221 atagtttcta ctgtggttat gagttcgatc ataattttct cctcctacaa cttcataata
     5281 gtagcatagc tctaaaaact ttccaatact tcttctattt aaagtcggat ttttgaaaat
     5341 tatttcacaa aaaatcaaaa taaagttcga tatattctgt ccaaatataa aaagagattg
     5401 tgacaaaaat cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca
     5461 caggccaagt aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt
     5521 gatcttacct tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag
     5581 caaagcctcg ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc
     5641 accatattca ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt
     5701 cacttgaata tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat
     5761 tgtatggatt tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa
     5821 gaattcaggt cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg
     5881 atctttagta acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc
     5941 tccgatattt gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga
     6001 atctgttaaa tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc
     6061 tttctccact tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa
     6121 ctgtccacct tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact
     6181 gtatttttcc ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat
     6241 gacttctgaa agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat
     6301 gaatgaacca attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg
     6361 tgaaactgct ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag
     6421 ttctaacaag cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc
     6481 cgatacaact gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt
     6541 tgcttcaatt gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa
     6601 ttgagcagca ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc
     6661 atcgatttct agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac
     6721 tttcgctgct gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat
     6781 ataacgaaca gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg
     6841 gtgctcttca tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg
     6901 ctcatctact tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt
     6961 ttgatattca tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg
     7021 tttcaagata ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg
     7081 caattcatca atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt
     7141 cattcgatct tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag
     7201 catcatcagg cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc
     7261 taacccttca gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt
     7321 ctttgtccgt cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac
     7381 tgggtccagc ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt
     7441 aggtgttcct tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat
     7501 tccttttcca gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt
     7561 tgaaaggctg atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa
     7621 taagctcatc aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct
     7681 gctagctaaa gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg
     7741 gtaagaacgg ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc
     7801 attcaaatcc atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc
     7861 tagtaaaaca tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc
     7921 aatttctaat actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc
     7981 atcttcataa cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc
     8041 atattcattg aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt
     8101 ttttgtaatt atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact
     8161 gattgtatca tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct
     8221 tacttttgca attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg
     8281 ttggatggta aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag
     8341 gtctgccatt tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat
     8401 atatgattca attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta
     8461 atattgacct taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa
     8521 ataaatcctt tagaatcaac aaaaaaaaat acggaatata ctattccgta tc
//
LOCUS       DARDHA010000121         8494 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000121 DARDHA010000000
VERSION     DARDHA010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.122"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..173)
                     /locus_tag="SDA10_002137"
     CDS             complement(<1..173)
                     /locus_tag="SDA10_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4241025.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="SDA10_002138"
     CDS             complement(593..1714)
                     /locus_tag="SDA10_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEG4241026.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="SDA10_002139"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="SDA10_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEG4241027.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="SDA10_002140"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="SDA10_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEG4241028.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="SDA10_002141"
     CDS             complement(4304..5215)
                     /locus_tag="SDA10_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG4241029.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="SDA10_002142"
     CDS             complement(5232..6239)
                     /locus_tag="SDA10_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEG4241030.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="SDA10_002143"
     CDS             complement(6236..8365)
                     /locus_tag="SDA10_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG4241031.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="SDA10_002144"
     CDS             complement(8370..>8494)
                     /locus_tag="SDA10_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG4241032.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DARDHA010000122         8434 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000122 DARDHA010000000
VERSION     DARDHA010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.123"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..270
                     /locus_tag="SDA10_002145"
     CDS             <1..270
                     /locus_tag="SDA10_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HEG4241033.1"
                     /translation="ITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMI
                     SSLNEARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            337..831
                     /locus_tag="SDA10_002146"
     CDS             337..831
                     /locus_tag="SDA10_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG4241034.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            887..1525
                     /locus_tag="SDA10_002147"
     CDS             887..1525
                     /locus_tag="SDA10_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241035.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            1597..1992
                     /locus_tag="SDA10_002148"
     CDS             1597..1992
                     /locus_tag="SDA10_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG4241036.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            1976..4429
                     /locus_tag="SDA10_002149"
     CDS             1976..4429
                     /locus_tag="SDA10_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG4241037.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            4426..6453
                     /locus_tag="SDA10_002150"
     CDS             4426..6453
                     /locus_tag="SDA10_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241038.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            6450..7472
                     /locus_tag="SDA10_002151"
     CDS             6450..7472
                     /locus_tag="SDA10_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEG4241039.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            7489..8418
                     /locus_tag="SDA10_002152"
     CDS             7489..8418
                     /locus_tag="SDA10_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG4241040.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
BASE COUNT         2862 a         1435 c         1692 g         2445 t
ORIGIN      
        1 attacagttc ctagagacga aaatctatct aaattatata gtaggataca acaattaatt
       61 gaaaaagaaa ttcaacctac tgaacttgct gatatagtga agacaacgat aaggtcatca
      121 aatggaatga ttagctctct taatgaagca agaaataggc attcacttgc acaccctaat
      181 acaaatatta ttggaaagag agaagcaaaa ctaataatag gtatttcaag tacagttaca
      241 gattatatta gcggatacct tgataagtag cgtcattaac tgattgctac tttttttatg
      301 actattttta taaaaaggat gtgataagaa ttggaaatga aagtctatgt ggcaaattta
      361 ggacggtata atgaaggtga attagtcgga gcatggttta ctccaccaat tgatgaggaa
      421 gaaatggcag aaagaatcgg tttaaatgag gactacgaag aatatgcaat acatgatttt
      481 gagcttcctt ttgatgtgga cgaatacacg ccgattagtg aaatcaaccg tctatgtgaa
      541 gccattcaag aaatcgaagg tacacctatt tataatgaac tcaaagaaat tcaaggcatg
      601 tggtttagta gcttggaaga attactggaa aacaaagagg atattcattg ttatagtgat
      661 tgtgattcga tggaagatgt cgcccgttat tacgtggaag aaacaggaca gcttggtgaa
      721 gtcccttcca atttacaaaa ttatattgac taccaatctt tgggacgtga tatggaaata
      781 gagggtaatt acctagttac ctctcatggg gtatttgaat attgtcagta agtttaacaa
      841 tccgtgttat aattaaatta ataaatagca cggagaggta taaattatgg taaatgtatt
      901 tgaaaatctt aaactattaa aactagacat gaaagataaa ggctgggtga tagattcctt
      961 ttactttcgt tacaaacagc aaaattatat tgtacttgtt aagctatttg aaaaagagga
     1021 aaaagttcca gaatatgctc tgttaaaact agaattttta aaggaaaacg atttttcaga
     1081 tatgctagct gtctatgcta attcggtaaa attatataca gacacgaaaa caataagaga
     1141 atattttggt atcgaataca gtagtaactt gggagatgtt ttatttcaat ttagccaaac
     1201 tcttgctcga tttattccga cagaagttag tgagaagaaa aatgaagacc aaaaagaagc
     1261 catgtgtttc tcattaagtc aaagtgattc agaagatccc agaaaaaaat attgtttttc
     1321 agttagaaga aatcctttaa agggaaatgg tgagctggga aaacgaagtc cttttaatga
     1381 caataaaacg agattgtata gacccagctt atatgaacga ttagggtcag ataccaattt
     1441 aagtttcaga tattcaatga atccagatga tgaagaaact gatgaaggta ttattgcaaa
     1501 atggacaaaa aataaaattg aatagttaaa gcattgaaag agcaatcacc tattttgatt
     1561 gttctttttc tatttttacg gaaaggacgt gaagacatga aaaaaataag aagttacacc
     1621 agtatttggt cagtagaaaa agtgatttat gcgattaatg atttacaact ccctttccca
     1681 attaccttta cgcaaatgac atggtttatc gtttctttgt ttgtcgtcat actctttggc
     1741 agtttaccac cactttctct gattgacgga gcactactca aatacttagg gattcccatt
     1801 gttctcactg ggttcatgtg ccaaaagacc tttgacggaa agaaacccta tggatttcta
     1861 aagtctatca ttacctatgc tctacgccct aaagtaacct acgctggcaa acaaatacga
     1921 ttaaaaaaag aaacaatgaa tgaatcgatt acgattgtaa ggagtgaaca actagatggc
     1981 atatccgatt aaatatatgg aagataatct cgtatttaat catgatggcg aagtattcgc
     2041 ttattatgaa ttaattccct ataattacag ttttttaagt tccgatgaaa aaattcaagt
     2101 tcatgataat ttctgtcaat tgattgccca aaatcgtgat ggcaagattc atgctctgca
     2161 actagcaacc gaatcaagta ttcgagccac acaggaacaa tcgaaaaaag aaatcactgg
     2221 acgattaaaa gagatagctt acgaaaaaat agatgaacag accgaagcgt taatttctat
     2281 gattggtgac aatcaagtag actatcgctt ttttattggt tttaaactct acctcaatga
     2341 acaggaagta tccgtaaaaa atgttgggaa agaaattcaa aaagcatttg cggaattctt
     2401 tcatggtgtg aatcacaaat taatgggcga ttttgtttct atgcctatgg cggaaatcga
     2461 acgcttttct aagatggaac agttgttaga aagtaaatta tccagacgct ttcagattcg
     2521 taaattggag aaagatgatt ttggttatat tattgaacat ctttacgggc aaacaggtgt
     2581 cccttttgat gactacgact accatttacc gaaacgatac gaagataaaa aagtgttggt
     2641 aaagaaatac gatattctca aacccactcg ctgtttaatc gaagaaaatc aacgctattt
     2701 acggattgaa caggaagata ccactactta tgtggcgtat tttacaatta atagtattgt
     2761 tggtgaattg gatttcccta atagtgaaat tttttattac cagcaacagc agtttacttt
     2821 ccccgttgat accagtatga atgttgaaat tgtcgccaat aaaaaagcct tatctaccgt
     2881 tcgcaataaa aagaaagaac tgaaagattt agatagccac gcatgggaaa gcgacaacga
     2941 aaccagtact aatgtggtgg acgctttaga atctgtcgat gaattagaaa gtaatttaga
     3001 ccaaaccaaa gaatctatgt acaaactaag ctatgtggta cgggtatcag caaagaattt
     3061 ggacgagctg aaacaacgtt gtgatgaagt aaaagatttt tatgatgatc tgtctatcaa
     3121 attagtacga ccttttggcg atatgctagg cttgcataat gaatttattc cctcaagcaa
     3181 gcgatatatc aatgactata ttcaatatgt cacgtcggat tttttggctg gtcttggttt
     3241 cggtgctaca caaatgattg gtgaaaattc tggtatttac gtcggttaca atctcgatac
     3301 aggcagaaat atttatctcc aacctagcct tgcaagtcaa ggaattaaag gctctgtaac
     3361 aaatgcatta gcgtccgctt ttattggttc attaggtggc ggaaaatcat tctctaacaa
     3421 tatgctggtc tattattcag tactctttgg cggacaggca accattgttg acccaaaagc
     3481 tgaacgtggt gaatggaaag aaaccttacc tgatattgcc catgaaatca atattgttaa
     3541 tctcacgagt gagaacgaca ataaagggtt acttgaccct tatgtgattt taaaactccc
     3601 gaaagattca gaaagccttg ccattgatat tctcacgttc ttaacaggta tttccagccg
     3661 agatagcgag aagtttcctg tcctgcgtaa agctattcgg agtgtaactc aaagcaaaca
     3721 acgagggttg ttattggtga ttgatgaatt gcgaaaagaa gatacgccca tcagcaatag
     3781 tattgctgac catatcgaaa gctttgtcga ttatgatttt gcccatctgt tattcagtga
     3841 tggcagtgtc acccaatcca ttagcttgga aaaacaactc aatattattc aagtggcaga
     3901 tttggtctta cccgattcca atagctcttt tgaagaatac actaccatgg aattattaag
     3961 tgtggcaatg ctgattgtca ttagtacctt tgctttagac tttatccact ctgaccgttc
     4021 catttttaaa attgttgacc ttgatgaggc ttggagtttt ttacaagtgg cacaaggaaa
     4081 agctctatct atgcgattgg ttcgagcagg acgtgccatg aatgcagggg tctatttcgt
     4141 tactcaaaac gcggacgatt tactagatga aaagatgaag aataatattg gcttgaaatt
     4201 tgcctttcgt tccactgacc tagtggaaat caaaaagacc ttagaatttt ttggtgtgga
     4261 caaggaagat gaaaacaatc agaaacgctt acgtgaatta gaaaatggac aatgtttagt
     4321 ttcagacatc tatggtcgtg taggggttat gcagtttcac ccgatttttg aagagttgct
     4381 tcatgccttt gataccagac cgcctgtgcg aaatgaggtg gaagaatgac acaaaaacaa
     4441 aagaaatttc ttcactatag ttggataacg ctcctttgtg tgggcgttat tttactttta
     4501 ttaggcacgc tgggcaatgc agtacaagcg acaggcttag ttgatgaaac cattgataca
     4561 agcaatgaat actccaagta tggtttgaat caatatcagc ttgattatta tgtggacaat
     4621 agctggggct ggcttccatg gaattggagt gatggcatag gacaatcggt gatgtatgga
     4681 ttgtatgcca ttactaattt tatttggaca atttctcttt atctatcgaa cgccacaggt
     4741 tatttggtga aagaagccta tgccttagat ttcattagtc aaacatccca agcaattggc
     4801 gaaaacatgc agacgattgc aggcattaca ccgaatggct tttccagttc agggttttat
     4861 gtcggtttcc tccttctttt tattctcatt gttggaatct atgttgccta tacaggattg
     4921 gttaagcgag aaacgacgaa agtgatacga gctgttttaa actttgtcgt ggtgtttatt
     4981 ctatcggctt cctttattgc gtatgcaccc gattatattg cgaaaatcaa tgatttttcc
     5041 aaagatgtca gtaccgccag tctgaatatt gggacaaaaa ttgttcttcc taacacggat
     5101 agtcaaggga aagacagtgt ggacatgata cgtgacagct tattctctgt tcaagtaaag
     5161 caaccatggc tattgcttca atatggctcc actgatgtag aagcgttagg ggaagaacgt
     5221 gtggaaagtt tattgtctac cagtccagat acaaacaatg gtgaagatag agaaaatgta
     5281 gtgattgaag aaattgaaga tcatgacaat acctatttaa ccctaccgaa gacgatttcc
     5341 agattaggaa cagtcttctt tttgtttgta ttcaatattg gtatctcgat ttttgttttc
     5401 cttctaacag gaatcatgat tttttcgcaa gtgctattta ttattttcgc tatgtttttg
     5461 ccaatcagtt tcctattaag tatgttgccg agttttgatg gcatgggcag gcatgccatt
     5521 accaagttat ttaatgtgat tatgacaagg gctggtatta ccttgattat taccgttgcc
     5581 tttagtattt ccactatgct ttattcgctg tcagcaagtt ctcccttttt catgattatg
     5641 tttttacaaa ttgtgacctt tgcagggatt tattttaaac tgggcgattt aatgagccta
     5701 ttcactttac aaagcaacga ctctcaaaac atgggcagac aagtgtttcg cagacctcgt
     5761 caaatgatga atcgacaaac cagaagattt caacgcagta tgagtaagat ttttcgtgga
     5821 aaagcagggt caacgaagca aaagacaatg acaacaaagc agaatcaaac caataaagct
     5881 aaccatactc gtcaaaatga acggactaca ccaaagaaag aaacagcact ttccaatgtg
     5941 aaaaagcaac aaggcggtat cgggcaacga ttaggtgaaa agacagccca tgtattggac
     6001 acgaaaaatc gtctagtcaa taaaagaaag caggcaaaac aacagataca ggcgacacca
     6061 acccatgtca aatatgccct tcatcaagga aaagagaatg tcactcataa tgtaacagac
     6121 tttaaacaat ctatggcaga taccaaagct caacgcaaac aacagcgtat gaaccagcag
     6181 gagcaaaaac gaaagaacat tgctcaaaag cgtttggagc tggataaagc aaaagaaaaa
     6241 agacgaaaag aaaacccatc atctcctgtt cagaataacc agcagcatgt tcgcaagcgt
     6301 cagcaaaccg tcacgcctgt tttgtcacaa aacactaaac gtgaaaatca tcagcgacca
     6361 ttgaaaaaag agcaaaaaca aaagacacca ccgaagcaat tcaatcagag gaagctaaat
     6421 aaaagaacgg ataagcgagg acaacaaaca tgaagctaaa aataacagtg atttgtagtg
     6481 tggtcttctc tctcttcttt tttctcttat tattcctagt cattttcttt gcagatgata
     6541 cggacagtgg cgaaaacaac aaggattctt ctattccaca aggcggtgtg accgtttcac
     6601 cagaagtgtt ggcacaccgt ccgttgattg aaaaatatgg taaggaatat gggattgaag
     6661 actatgtttc ttatatctta gccattatgc aagtggaatc gggcggtaca gcagaagatg
     6721 ttatgcaaag ttccgaatct ttgggtttac cgcccaacag tttgagtacc gaagagtcca
     6781 tcaaacaagg ggttaaatat ttcagtgaat tattaaccag tgcagaacaa caaggagtgg
     6841 atatagattc tgttatccaa tcttataatt atggcggtgg ctttttaaat tatgtgagga
     6901 gtcatgggaa aaaatatacc tatgaattag ccgaacagtt ttctaaagag aagtcgggtg
     6961 gtcaaaaagc agactatccc aatcctatag ccatacctgt gaatggtggt tggcgatata
     7021 actatggaaa tcagttttat gttcagttgg tttcacaata cctaaccgac acttccccaa
     7081 cagaatttga tgatgaaacc gtgcaagtca ttatggacga agccttgaaa tacgaagggt
     7141 ttccctatgt ctttggaggg gcgtcgccta ccacctcttt tgattgtagt ggcttaatac
     7201 aatgggtgta tgacaaagct ggcatttctc tccctcgtgt cgcccaagat cagtatgacg
     7261 ctacgcaaga aatctctatg gaagaagccc aagcaggcga ccttatcttc tttcattcaa
     7321 cctataatgc aggcacatat gtgactcatg tggcgatata tttagaggga aatcgctttt
     7381 atcatgcagg cgacccaatc ggttatggcg acttaagcag tcgttattgg caagaccatc
     7441 tgattggggc tagacgtgtt attcataact aagaaaggaa tgaatcaaat gattataaaa
     7501 atcgagcgaa aagaaaagaa ggagaaacca aagaaatcta aaaaaccgag caagcaaaga
     7561 aaagttccga tgattaaagt tggcacgcat aagaaattaa cctttgtttt atgggtttta
     7621 ttgattggta gtgtcggatt tggaatctat aaaaatttta ctgccattga cactcatact
     7681 gtccgtgaaa cagaaatcat taaacagcag attgtcgata ccaaccaagt agaaagtttt
     7741 gtgaaatcct ttgcgaaaga ctacttttcg tggcaacaat cccaagaagc cattgataaa
     7801 cgaaatgaaa aattgaccca ttatttgacg gaagaattgc aggtattaaa tgaagaaatg
     7861 attcgtaagg atattcctac tagttctagt gtgaacgata tacaggtgtg gcaggtttct
     7921 caagtgaatg agaacacctt tgaggtactg ttttctgttg aacaagttat taccgaagac
     7981 aaagacaagg aaactatatc gtctagcttt catgtggttg tccatataga tgaatcagat
     8041 aatatggtta ttatcaaaaa tccgacaatg agtaagaaac ctcaaaaatc ggactaccaa
     8101 cccaaacagc tagaaagtga ccatacagta gatacagaaa cgatggacga aatcactagc
     8161 tttttagaaa cgttcttcca gctctatcct accgccacag aaaaagaatt gacttactat
     8221 gtaagtaatc atgtgttacc tatgattaac aaggaatatg ttttcgagga attggtgaat
     8281 cctatcttta ccagaaaaga taaccaagta atcgtgaatg tcgctgtgaa atatttagat
     8341 caagaaacaa aggcaaccca aatctcgcaa tttgagttga tattagaaaa acaagataat
     8401 tggaagattg taaaataaca aatattggta catg
//
LOCUS       DARDHA010000123         8342 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000123 DARDHA010000000
VERSION     DARDHA010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.124"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            195..674
                     /locus_tag="SDA10_002153"
     CDS             195..674
                     /locus_tag="SDA10_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241041.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            707..2935
                     /locus_tag="SDA10_002154"
     CDS             707..2935
                     /locus_tag="SDA10_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4241042.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            3064..4707
                     /locus_tag="SDA10_002155"
     CDS             3064..4707
                     /locus_tag="SDA10_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEG4241043.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(4780..5325)
                     /locus_tag="SDA10_002156"
     CDS             complement(4780..5325)
                     /locus_tag="SDA10_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEG4241044.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(5348..5833)
                     /locus_tag="SDA10_002157"
     CDS             complement(5348..5833)
                     /locus_tag="SDA10_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG4241045.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(5823..6635)
                     /locus_tag="SDA10_002158"
     CDS             complement(5823..6635)
                     /locus_tag="SDA10_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEG4241046.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(6774..8306)
                     /locus_tag="SDA10_002159"
     CDS             complement(6774..8306)
                     /locus_tag="SDA10_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEG4241047.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
BASE COUNT         2813 a         1588 c         1643 g         2298 t
ORIGIN      
        1 acatcatctt ggactttcta catgttacta atgagcgtgt aaaagaacga gatggtgttt
       61 ttatttctat caatgaagct ctttttcttt gcaatcctca cttcaatcct ctataatgtc
      121 gcatatagtt tagtaaatga gtaaactact aatttactta tttactaaaa ataaaaggat
      181 gaggagttaa aataatggaa ataccaacaa atttaaaaca taaaccagta atcgttgtag
      241 aagactatga taaagttgat ggaagaaatg cattaaatat ggatgcaaaa gggctttcac
      301 tgggccttgc tcagtggaat gatcgtggaa aagtcgatat ctcaggcaag gtatggcgac
      361 atactggaga aaaatggtca cgacagtcgg aagaattacc aatcacacgt attttagatt
      421 tagcgatctt agctgcgcaa ggaagtttat acttccaaga tgcttatcgt tatgaaaaat
      481 tttatgatcc agaaaatcca gtcgtagaca tcattggatt acaagggaac cgaatgacgg
      541 tagaagtaga taccaaaaat ccaaagatcg atgaagacat cattttgtat tacgatacac
      601 tacaaaaaga cggagaactg attggggaac gattccgtat tttaaaacgt cttttagatg
      661 agctaggata ttaatagaca gagatgatca aaaggggaag agaagcatgg agaatttctc
      721 actaacagaa aaccgaacct tttcacggct agaaaagcaa atgatctgga agaaaccgaa
      781 aagccatcgg acaagtgcag aagaatggcg gatcgcctcc gaaatcaaag ggaattggca
      841 agatcctgaa atgaaaatca gtaacgtact tttggaagga gatgccgggt cggggaagac
      901 acaattagcc aaagcactat ctgctgatct gcaacttccc tatacaaaag tgacctgttt
      961 tgcagatatg gacaaatcag atgttttcgg agccttatta cctgtagtag attctaataa
     1021 ggaacaagac caagaattac tagaagctat ttaccaaaca gatacacttg aagctgtact
     1081 gacactgatc cagaaccatt acggcattga tcctttttct gctaaagaaa aattagcaga
     1141 gctagttcaa aggatcgaag ctgattatcc agatgcaatc cattacagct attacccttc
     1201 tgaaatcgtt cgagcgattg aaaaaggcta tttattagag attcaagagc cgactgtcat
     1261 ccgagatgcc tctgttcttg tggcacttaa ctcagcgctc gaaccaaatg ggatgttgaa
     1321 tttaccgaca ggaatcattc gtcgccatcc agattgcgtc attgtgatca ctacaaatcg
     1381 aaattaccaa ggaaatcgcc cattaaatga atctttaaga gatcggatgc aacatgcgga
     1441 aaaaatggat cttcctgcct tagaagtcat ggcagaacgt gcgatggcga aaactatgat
     1501 ccaaaaacaa gaacttctct taaagatggc agagattatc cgtttgttgg atgaaactgc
     1561 aaaagctaac gccatcaaag gtgtagcagg aatgcgttct tatttttatt gggcaaatac
     1621 ctataagcaa ggacaagatt tactccagtc gatttatcca aaagttttgt ataaattatc
     1681 cactgattca gatgaattgg caattcttga acaagctttg tctgacagtg gattgctact
     1741 ggaacttcag gacatattga gaaaagaaaa atggggtcat cctcaatctg agcatacaaa
     1801 agaaagaacc attagcgccg aagaagcaaa tgaacgaaac atcactgaaa atgctgaaga
     1861 aattctgcgc ttagatgaaa agaaatcgtt gtctgctgaa gaaccaaatg agggagaaga
     1921 gaaacaagaa gaaacaatag aaaaaaacga gattcctcct caaatgcaaa aagaacgcag
     1981 tagcgatatc gaaggaaaaa atcaaaaaga acaacaaaca ggagatgatt ctcaagaaat
     2041 gtctgcttct gacatggaag cacatgataa ggaaatcaag aagcaactga ataaagaagc
     2101 gcgacaaatc atgaaggaaa cgattcatga aaaagaaggt ctcatcgttc accgaccagc
     2161 tttccaagga aaaacgccgg aagcacgtga gctacacatg caagtaatgc caatagtaga
     2221 atcacttagc cggcaaattt tagagttact ggataatgaa caaacagaga cgtaccaaaa
     2281 aggaaaatac gaaggtcagc ggttcaatgc aagcaaagta gcatatggtg atttgagaaa
     2341 ttttgacaaa aagaacccgc cacatgagca accttcttta gcggtagctg tacggatcga
     2401 tgaatctggg tcaatgatac gggatgatcg gatcgaagcg gcaaaaaaag cagcgattgc
     2461 cattgccgaa ttttctaaga aagtcgatat cccattgttg atttacggag atacagcaga
     2521 ccgttcgtca agagaaaaga cttctttgtt ttcttataaa gaatttgaag acggatttca
     2581 atggttaaat gaaaaattag tcactatgaa accaaggcaa aacaatcgtg atggtgctgc
     2641 acttcgttta gcagcagaaa aattaaacag acaatccgca acaaccaaac tgttgcttaa
     2701 tattagtgac ggtcagccaa aagcattgcc ggattataca ggcaaaaaag caaaagaaga
     2761 tatccagtct gttttgaaag aatatgaacg tcaaggaatc ctatttgtct ccgcagctat
     2821 tggccaagac aaagaagaaa tcaagtcgat ctacggtgaa agtcgttttg tagatattac
     2881 tgacttgaat gaatttccca agcaaatgat tcaactgatt gcccgctatc tatgataaaa
     2941 taaagctcaa cgaacgcact tagcagattc gttgagcttt tcgtttctag ttaccggtaa
     3001 caaggatact tctaaaatag aatcggttat aatcaagagg gaccgttaaa ggagtgaaac
     3061 gatatgatac aagaacaaaa atggtggaaa gaagcagtag gctatcaaat ctatcctgct
     3121 agtttcaaag atagcaatgg cgatggtcaa ggagacatca atgggatccg tgaaaaactt
     3181 aattatttga aagatcttgg tatcggcttt atctggatca atccgatcta tcaatctcca
     3241 tttgtcgata atggttatga cgtaagtgac tatcaagata tagaagagag attcggaacg
     3301 atggaagatt ttgatttatt gctaaaagaa gcacatgaat tagggatcaa agtgattatg
     3361 gatcttgtca tcaaccatag ttcggatcag catcaatggt ttgaagaatc gaaaaaatct
     3421 aaagaaagcc cgtatcgcga ttattacatt tgggtcgacg gggtagatgg caaagaaccg
     3481 aataattgga catcgatttt cggcggttct gcttgggagt attctcatga aactggacag
     3541 tattatctgc atgtttttgc aaaagaacaa ccagacttaa attgggaaag cgagaaattg
     3601 aaagaagaac tattcaatat gatccgatgg tggttggata aagggataga tggatttcgt
     3661 ttagatgcga tttctcatgt aaaaaaagat gaatattcag taaaagccac agagaaccct
     3721 ttctcaccct ttcaaaatgt ctcaggaatc gaagaacatt tgaccgattt gaaacatgtt
     3781 tttgaagagt acgatatcat gactgtcgga gaagcaagtg gtgtcactgc agaagaagga
     3841 ccacaatggg tagggaaaga cggttatttc gatatgatat tcgaatttga tcatatccat
     3901 atctggcagc aagaaaaaga agggcaatta gatgtcctta aattgaaaca tgcattaagc
     3961 gcttggcaaa cttcattgga tggtatcgga tggaacgctt tatatatgga aaaccatgat
     4021 gttcctcgtg ccgtttcagt gtttggagat actcgtcccg atttttgggc tatgtctgca
     4081 aaagcaatcg ctatgatgta tttctttttg caaggtacgc catttattta tcaaggacag
     4141 gaaatcggaa tgacgaatat gccctttgaa tcgatcgatc aagttgatgc agtagattcg
     4201 aaaagacttt ataaaaggct gcttgcggaa ggtaagacaa gagaagaagc attggatatc
     4261 attcgtgaaa caacgagaga caacagcagg acacccatgc aatggacttc ggaacaatat
     4321 gctggttttt ctacacatga accttggctg atcactaatc caaataccaa aacgatcaat
     4381 gtagaacaac aagaatatga accagagtcg gtcctgcaat tctataagaa catgatccgc
     4441 attagacaaa ccaataaagg tctgatatac ggttcttata aagaatatct tcatgaacat
     4501 ccacaacttt atgtctatga aagatattta gaagatgaac aatatcttat tatggtaaac
     4561 ttaacagaat cacttgctga ctatgaactt ccaaaagaag cagatcagtc ctggacatta
     4621 ctcttgtcaa attcctctag cggagaattt gaagctaaag gcatactagc accttatgaa
     4681 gctcggttat ataaaacaaa caagtaaaac ttcctcgtta aagttcatta gttggacttt
     4741 gcgaggaagt ttttcagatt gctctgtcaa ttactcattt caataattcg ctctaggacc
     4801 accgatatat ttaggacgag agcgaagggc tgtcacgtga gcttgtccaa gggttttcaa
     4861 acgaggtctg acaggtactt ggacatgctt gatatgcata ccgattgatg tgtcaccgat
     4921 atcgataccg gcttgtccaa ctacgtgttc cacttcgaca ggatcattga actggtcaaa
     4981 agcagcaaca gaacaagcac ctccagcatg tagtgcagga acaacagaga caatttcgaa
     5041 attttttgct tctgccactg ttcgttctac tactaatgct cggttcaaat gttcacaacc
     5101 ttggacagct agcgcaattt ctttaggatc caacaactct tttaacgtac ggatgatcca
     5161 ttgaccaact tccgcactgg aattttttcc aatatgtcct ccaacgactt cgcttgtgct
     5221 gcatcctaaa acaaaaagat cacctttttt caagtgtgcc tcagccaata tgtcattaac
     5281 tatttcagtc aattggtctt ttaattcttt ttctgacaac ttcattttct aaaaccttct
     5341 ttttcaatta atattgcttc cgtgcttgaa tacgttgaag acttgcagtt ataggaaagg
     5401 caagtcccat tccaatcaga ttttggaaga tgtttcctgg tatagaagcc actccagccg
     5461 gccaatcata taaaatccaa ccggcgatac catagccgat gatcataact aagcttccta
     5521 ataaaagccc taatccccac atacgagtag aagatttgct gaaagaacca gcaacatatc
     5581 cttgtaaccc gtggatcact aatgaaaaaa ctgcccactg cggatatcct gaaatcaaat
     5641 cgatcaatag accactggaa gcaccaacaa ctaatccacc cgttggacca taaagaaaag
     5701 cagcggtata gatcccggcc tcgcataatg tcacaaatcc attcgtagcc ggtacaggga
     5761 tcaaaattgt taatgaaagg gcaactgtca acgcaatcag catagcatag atcgttaatt
     5821 gcttagtttt catttctcat tcctcctttt tgaataccgc cagaccagac acttccggta
     5881 tgatcgttca tcagcacgcc attagaaata caatgggcaa caaatttttt actttctgct
     5941 actgctttgg taaaatctgc ccctaacaca agattcgctg ttattgcaga agaaaaggca
     6001 caaccggccc cgtttactgt tgtagtgtcg atttttttat gagaaaatac ctcataatga
     6061 tcaattgaat agagaagatc cacagcttca tctccagcca tccttgtgcc gcctttgatc
     6121 acaatgtttt ttgttcctga agcagtgagt tctttagcga gatggatcat ttctgttaac
     6181 gaagagggaa gagcttgacc agaaagtaaa gcagcttctg ccaaattagg cgtaatcatc
     6241 gtaacaagtg gaaaaagttc ttcgatgagc tgcgaaatat acaattgatt gatcacagct
     6301 tcagtttctt tgaatgccaa aacagggtct aaaatgattg ggatattttg ttttgttttt
     6361 atgaagtctc gaacaaccat gattgcttca cttgaattca ataacccaat tttgatacca
     6421 tctaatgaag tcattttatc aatcgaaact aactgtcgtt taagcaattc caaagatata
     6481 gattccagca taaatcgctc attaatatct agtgccccta tagaagtcaa ggcgcttagt
     6541 ccgaatagac caaaattctc aaaagttttt aaatctgttt ggattcctcc accagcaaat
     6601 ggatcagaac cgccaattgt caagattctt ttcataacat tttcctccat gaataagagt
     6661 ataacgaaat tttcaaaaaa gaaaaacagc caattggcat atttttatct catccaattt
     6721 aaaaaaacaa aaagaaaaga aagaccaaaa tggatctttc tttccttaaa tagttaggac
     6781 ttttgtgatt ttaattcttt ctcgatttga agccgttgca ctttcattgt agctgttcgc
     6841 gggattgcat caaaatcacg gatgataggg acgttcaagc gaggaagttc atggacttgc
     6901 tcccaccatc gattccaatc catttcttta tcaggagcta aagcaataat cggttgtgga
     6961 gaatgatttt tatcccttac aatcactact tctgccaaaa aatctaacga atctaataaa
     7021 aagtcttcaa tagctaatgt actattgatc gtttcgatca aatccacttg ccgatctttc
     7081 aaaaataggt ggccatgctt atccataaaa ccgtaatctc cgctatccca ccaatcgcca
     7141 taaacatttt cctggaaccg tgcatcttcc ttgtaataag tcaaagcccg tcctttagac
     7201 agtaattgta tatgaccatc tgtgttttca ggtaggacat tcccaactga atctgtaatc
     7261 cgagcgcttg tcatgtcttc cagtccaacg cccatatctc ttgcatcact tgttttcaaa
     7321 ctctctagtg tatgagcctt aagaatcatt ggtccgcatt cactttggcc atatacttga
     7381 agaaataccg catcctgtgc catcgacgtt cgcaaaaatg tcttcattgt ctggttgttt
     7441 atagcatcaa aagtggagtg atagtatcgg atacccgaaa aagcttctgg atgctcctta
     7501 gcagtaaaac gccactgaac aaagttattc ggatgggtct ctagtgcaat tggccggtga
     7561 gcttccaaca tatgaacaac ttttgaactt tgtgcgtttg ctaaaggcat cataggaaat
     7621 cccatggaca tcaaagaaga gataccaata ttgaatctgg aatgcactgg agagatgtga
     7681 aaagcgatta atttcttttc cgaaattttt gtgaaaactg ttttttgcca tttcgtccgc
     7741 catcccatag aatgagcaga atgacaaata agtttaggga taccagttgt accagaagta
     7801 tgggtcatat aagaaatttc atttttgtct aaaaatgatt gagaaacaga caaagcaggt
     7861 tgttccatta aataaagaat aggaatctgt tttgattttg ttccatttga aatttctctc
     7921 actcgctttt ttgtctcatc atcaaataaa atgaaagggt cttctaaacg atcgataaat
     7981 acttccattg ttgttgttgg tagatgataa gaagtcatga caggcactgc accaaggtaa
     8041 gacacagaaa cagctagaag gtatgtgtca aaagcggaac tcttataaat aataactttg
     8101 tctccggatt tcactccaag atgagccaat tgataagccc gtttcaacac actttcgcag
     8161 cattttttgt aagttgtttc aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag
     8221 ataatcgggg tctcggaaaa gttagctgca gcttcttgaa aattcgtaaa taagttcaaa
     8281 ggggaatacg ttatggtctc catcattcac actccattat cttttgttaa gtaatataca
     8341 aa
//
LOCUS       DARDHA010000124         8060 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000124 DARDHA010000000
VERSION     DARDHA010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.126"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            476..1339
                     /locus_tag="SDA10_002160"
     CDS             476..1339
                     /locus_tag="SDA10_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG4241048.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="SDA10_002161"
     CDS             complement(1468..3090)
                     /locus_tag="SDA10_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4241049.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="SDA10_002162"
     CDS             3339..3860
                     /locus_tag="SDA10_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEG4241050.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="SDA10_002163"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="SDA10_002163"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEG4241051.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="SDA10_002164"
     CDS             5941..6759
                     /locus_tag="SDA10_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEG4241052.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="SDA10_002165"
     CDS             complement(6862..7059)
                     /locus_tag="SDA10_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241053.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="SDA10_002166"
     CDS             7228..7806
                     /locus_tag="SDA10_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEG4241054.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="SDA10_002167"
     CDS             7901..8038
                     /locus_tag="SDA10_002167"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241055.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2406 a         1467 c         1593 g         2594 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt
//
LOCUS       DARDHA010000125         8018 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000125 DARDHA010000000
VERSION     DARDHA010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.127"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            125..1960
                     /locus_tag="SDA10_002168"
     CDS             125..1960
                     /locus_tag="SDA10_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEG4241056.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(2020..2736)
                     /locus_tag="SDA10_002169"
     CDS             complement(2020..2736)
                     /locus_tag="SDA10_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HEG4241057.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(2963..3505)
                     /locus_tag="SDA10_002170"
     CDS             complement(2963..3505)
                     /locus_tag="SDA10_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEG4241058.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(3510..3737)
                     /locus_tag="SDA10_002171"
     CDS             complement(3510..3737)
                     /locus_tag="SDA10_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG4241059.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(3816..5696)
                     /locus_tag="SDA10_002172"
     CDS             complement(3816..5696)
                     /locus_tag="SDA10_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEG4241060.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(5885..6499)
                     /locus_tag="SDA10_002173"
     CDS             complement(5885..6499)
                     /locus_tag="SDA10_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4241061.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            6641..7201
                     /locus_tag="SDA10_002174"
     CDS             6641..7201
                     /locus_tag="SDA10_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4241062.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(7506..7820)
                     /locus_tag="SDA10_002175"
                     /pseudo
     CDS             complement(7506..7820)
                     /locus_tag="SDA10_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT         2441 a         1594 c         1300 g         2683 t
ORIGIN      
        1 catcgaatac ttcgcttttg attcgattga acaattaact cagcgttgga aaaaaagaat
       61 tcattggctt tttgttcata aaagttattt tttagttgca gcattttttt actgctttac
      121 cttcgtgcag attttcgttt tagaaccaaa agaaggttgg aaagaaacta tccaggttgc
      181 cttaaaaata tttactcagc gacaagcacc catgatcctg acaatcatca ttattatggg
      241 atttttcttc ttactattgt tgatttccaa tcgtttttgg tacgccttga ctgctacctt
      301 gatcattaac ttactgctca ctatttcgac agtaatcaaa atggaaatgc gggaagagcc
      361 tgtctttcct tcagatttaa aaatgctgac cggattgtca gaactattat cgatggttag
      421 cccagtctta ttaatcgtgg gcggattgat ccttctccta ttattgatta ctagtattat
      481 tgtacaacgt agactacaaa aacagtattc cttgaagatt cattggaaac gtcgatttat
      541 cagtatcgct gtattattag gatgtttttc gggtgtcttt ttcattaatc acaaaaattc
      601 gccttctttc ttgctgttta atttatttaa agtaaacaag actttcttta accagcaagg
      661 cgccgtaaaa gataacggac cagtcattca attcctaaac aatatcgaca ttaaaatcat
      721 ggaagaacct gctggctatt cgaaagaaaa aattaatgga ataatgaaaa agtacaacac
      781 aaaggcagat gaaatcaacc aaacaagaag tgactggtta aacaatcaga cgatcgttct
      841 caatttaagt gaaagcttca gtgatcctgc acgtgtccct aatctgacag tacctacaga
      901 ccctattcct tacatcaaaa gcattataac agaaggaaca ggaggattga tgttatccgt
      961 cggttatggt ggcggaacag ctaacatgga atgggaagga ctgacaggct tgagtatcag
     1021 taacctgtct gcttcgttag ttacacctta tacccagcta gtggagcgtc agaaaataag
     1081 tccaaatatt accgatctat tcgatgaaaa aatagccatc catcctttta cagctgctct
     1141 ttatcgcaga aaacaagtgt ttgataaatt tggttttgac aggttctatt atttagacag
     1201 tccggataaa ttaacttata cggataaaat agaacaaagc ccacgcatat cggatgattc
     1261 agcatacaaa gaaaccttga aagctttaca aagcaatacg gaaacgtcac aattcatcca
     1321 attatccact atgcaaaacc atatgcctta tgatgattac tatagtgaat tgaactacac
     1381 tgctgaagga actgctgtca ttcctaatag aagaaaagaa ttacaaactt atatgcaagg
     1441 ccttcactat accgatacag cagtaaaaaa attcatccaa gaaatcgaca aaatcgataa
     1501 accgattacc tttgtttttt atggtgacca tttacccgca atctatagtg ggaatgattc
     1561 gaaaaaatat ggattagaac agcaccagac agattatttc atttataaca acaagtatag
     1621 tcgtgaacaa gcagctacgt taaataaaga agttgttgct cctaataact ttggtgctat
     1681 ggctcttgag catgcgaata tcaaagtaac tccttactat gcgttgttga cagaagtatc
     1741 taatcatcta cctgcattaa caattgatcc tacagaaagc ttgtctaatc gttttaatgg
     1801 aaaacaggtc tttgttacag aggatagtca aattatccaa gaaaaaaatt taactgagaa
     1861 acaaaaagaa attctagaag attatcgtct gatacaatat gatcttgttg caggtaacca
     1921 gtacagtgca acatgggcga cacaaaaaat aaaagactaa caaataaact gagcaagggg
     1981 ttgtgataaa agacatgtca caaccccttg tttatctcat taatttgctt gtccataaac
     2041 gatttgatta aagccatttt tcaattcatt attcgctgtc tcgttgaagt aaagataatt
     2101 atcgggattc ccctgatttt gttgtgtcac caatactttt ggctgatttt gctgaaaccc
     2161 gaatagatag acgtgctttt tcagatatgg ttctgtctct gcatcggaat agacggctgt
     2221 gatttgatag tctgcttgac ctgtcccact ttctgaccat tctgcagaaa caggcactcc
     2281 accaatagcc atcttccact ccccgttcaa aatagcttga gggactttaa gaccatataa
     2341 gtccacactc atttgattcg tataactttt gtattgttgt cccattgttt ggccccattt
     2401 ggtcataaat gcctctaatt gtgcggattt gtctgcattc cacagtgttt gttcaacttg
     2461 ttcgctgctt tctgaagagg aagatgaagc ctctacagaa gaacttgaag tagatgtttg
     2521 agttgtttca gtcgttttgg aagactctga cgaagtagag gtatctgctg tttctgtact
     2581 tgctgcagtt gacttgctct ctgtgctagt tgttgtagta gttgcaactg ttgctttcgc
     2641 tgtttctttt gttgattctt ttttcgattc attcccgttg gtacaaccag ctaacaaaaa
     2701 agctagacca attgttacta aaatcctttt tttcaattaa aaaactcctt tcgattactc
     2761 ccacttcttt attctatcaa ataagcaatg tgattttatt gatttttcgt attttctcat
     2821 caatctttca aatcttctaa aaactgagaa gcacccgctg atgttgtctg taatacatca
     2881 gcgggtgctt cttttatttt aatcttcgtg attttccaat ttacctcacg ctttatattt
     2941 gtatttgaag aaacatagac ttttagtcat catcgtcttc ctcatccaaa tcttgtcgga
     3001 cagtttttcc ttggagtctt tgtacttggt agatgatttc tttattgaaa cttaaaagtt
     3061 caacaagcaa ttgttgcaag ccatattttt cttctttttc agctaatttg attgcccgtg
     3121 tgataaagac attttggaaa tcaaaatctt ttgcaaagtt ttccaacatt gttgaggctg
     3181 gtaaatattt gttttgacca tgttcgtaaa tttttccata acgcatgact tcttctaaag
     3241 tagacggaac gatttcattt tcaatcacta actcatgaga gattttattg aaccatctcg
     3301 cttcttgatc agcatattct gaaaaaaggg tacttaattc ttgcgaagat gtacccaaaa
     3361 catacaattg tgcttggatc aatttcattt cttctaattt aaaatttgct aatacatgcc
     3421 ccaccatggc gccagcagtt ggtttatgat ggtcgatatc tgcttgtttt gtttcttgaa
     3481 caaatttttc ttccactata ttcattgatt tacgcctctt ttacttttgc tgattggact
     3541 tcataaccta atttttccac aactgcagct aattgttcag ggtggacttc atctggatca
     3601 aagtttgctt tgattttgct cgcattaaac aaaactttcg tattgcttac cccaggctgt
     3661 tgagaaaccg catgttcgat tttttgcata cagcttggac aagctagtgt atctaattgc
     3721 ataataattt ttttcatcgt agttcttcct tcctttttct tgatagtctt attctagatt
     3781 acagcttcca taaaaaaatt gattcttatc aagttttacg atttttcttc tttgctttcg
     3841 tgtccaaaat acaataaacg catcgcgtta aagataacga tcaagatact agcttcgtga
     3901 acgaacatcc cactagccat gtaaataaac ccggcaaata agccgttgag taaaaataag
     3961 acgacaccaa tggcaatcac tacattttca cgggtattta aaacagtttt cttagccaag
     4021 cggtaggcat gaaccaacga ttcaaaatta gattgcatca agacaacgtc actcgtttcg
     4081 atagcaacat ccgttcctga tcccatagca atcccgatat cagccgcagc caaagaggga
     4141 gaatcattga ttccatctcc aataaaagca acgcgcaatc cttgttcttg gtattttttc
     4201 acaaattgaa ccttttcgtc tggtaataat tcagcatgga cttcatcaat gcctaacata
     4261 tcagctacat aatcagcagt catctgatta tcacctgtca gcatcactaa ctgttttgct
     4321 cctgcttgtt tcaacttagc taattgttca gcgacttctg gtcgaatcac atctgacaca
     4381 cctatgattt gagtgatttt tttgtcgatt gctacaataa tgaccgagct gcctgtttgt
     4441 tgtaattgat gtaaatcttg tacttgcttt ggtgtgagag aaagtttgtg agcggcaatc
     4501 aatttggtgt tgcccgcaca aactgtatga ccatcaatct cagctgtgat tccttgtccc
     4561 ttgattgtct gattatcttt gactgctaat tgttgaaaat caatgtcttt ctcctgagca
     4621 taatggatca cggcgcgccc cagaggatga tcggataacg tttctaatcg agccactaaa
     4681 gctaagctgt ccccttcatc ctctgtatag ttcttgatag tggatacagc agtagttcct
     4741 ttggtcaatg taccagtttt atcaaagaca aaagtatcga ttttggaaaa agtatccatt
     4801 gcttcaccgc ctttgactaa gatgccccgt ttcgctccat taccgattcc tgctacattc
     4861 gaaacaggcg caccaatgac taaagcgcca ggacaaccaa gaaccaaaac agtgattgct
     4921 aaacgaaaat cacgactaat cagaccaaca accaacgcaa taagtaaaac ggttggagta
     4981 taatactgag aaaatcgatc aatgaatttt tccgcatggg atttggtatc ttgtgcttct
     5041 tctactaatt cgataatttt ggcaaaggtc gtatcatctc cgaccttagt tgcttcaatc
     5101 gtcacgtagc catctgctaa aatcgttcca gagaagactt catctccact gattttgacg
     5161 acttgtcgtg attcaccagt cactgcggct tcatccgcat accctttccc gtcaatgact
     5221 cgtccgtcca caggaatagc tccaccagtt tttactaaga cgatatcgcc ttcttccacg
     5281 tcatcgattt ccacttcttc agtcgttcca tcttcttgga caacactagc agtagatggg
     5341 gccatctcgg tcaaagcctt gattgactcc cgcgttttgg tcaatgtttt ttgttccaga
     5401 tagctaccaa acaaaaacaa gaatgtaacg atagctgact ccttgtattc tccgatcata
     5461 aaggcaccaa gcacagcaat agtaacgagt aaatcaattg agataacttt gactttcact
     5521 gcctgatacg catggatcat aataggtatg accgcaataa tagaagctat ggctagagca
     5581 atgttataga acaagacaaa accagtaaat tttccgataa aaccaaaaat aatcaacaca
     5641 gctgataagg cggtgatatg atttcgatgc tcttggatcc acttttgtaa tttcatcttt
     5701 ctccctcttt tcctttcatt cttacgagac taagtataga tacttatcca tcataaaaaa
     5761 ttgaccacta tcaagtttag tcaaaaaggc ggttttcttt tggtagaata cactacaaaa
     5821 aatagttagc aagaagactc actaaaagtg atcttgccaa ctattgtcgt ttctatcgaa
     5881 attttcattg aatgattttc ctgtacagca tacatcggag atctatttct ttatctaaga
     5941 tttttatctt gctaattttt tcctttagtc tttcaaatcc acgtcgttca tagaattgat
     6001 acgtacagcc actatctgta aaaaggaata tttcttttcc tttctctctt ttttcaaatt
     6061 ctgttagcaa cttggtaccg atgccttttg ttttcaaatc aggatttgca gctaaaaaaa
     6121 tgatctctcc atctggcgaa tgcttcttta aatatgctgc aagcatttgt tcatttgctg
     6181 tctcgtaagc tcctttactt tctttagcaa atatattttg taaaaaatcg aatatcttaa
     6241 catatatttg ctgtccaaaa gaacgatgtt tctttttttc tccttttatc tccgccagca
     6301 aaacacctgc caactcgtcc tctgcataca aagcaagaat ttgagttgct cgattcaact
     6361 ccatatacaa aaaatatctt ccgtaaagat tcagcagaag ttcattttcc atatatctct
     6421 caaagttcat tccttcaaca gcaaaataga tagctttttt gtaatctttc ttttctaagg
     6481 gtttaaaagt tagtttcatc atttcgcctc ctcaacttca atgattgtgt tatacttaca
     6541 ttataataga cacagtgtct actatctata atcaatcatc tataagtttg tcgattaagc
     6601 gacagctttg ctttgatatg tcggataagg agataggtaa atgaaaaaga cagatagacg
     6661 cgtcaaaaaa acagaaaaag ctttggctga aacactatcc acattattag taaataaaaa
     6721 gattcaagca atcactatac gcgaactaac agaaacggca gatgtacatc gttcgacttt
     6781 ttatacacat tataaggata tctatgatct atatgatcaa ttagaaagca acttttttcg
     6841 ggatctaaat gaaattctct cttatgatcc agcccatagc tatgaggaac tatacactcg
     6901 cttgattgac tatgtttacg caaatacttt tttatatcgc ttatttatcc tcaaccatgg
     6961 tgatatttcg tttcagaaga aagttaaaca agttattgaa gaaaactatc tgaacatatg
     7021 gctatatgaa gacaagaaaa cgattatcac agaagaaatg agattcttta ctacgtacca
     7081 cgtccaaggt tgtctatccg ttctcaatcg gtgggtagaa agcaattaca agtactccaa
     7141 aaaagaatta ttagaactct tacagaaact gaatgatcac atagagaaaa tcatgccttg
     7201 aataaaaaca tactaagctt agttaagaag aaccgtaacc ttctcgtata acaactgaat
     7261 cttatttagc caaattgttt actgaagatt ttttgagtaa aatttctata attcctactt
     7321 attgaaaagt agtttgtagt gctgcttctc cctctctaag tgatatatct tgttgtacca
     7381 atcaaattca cctcatcatt aataaattac ttttaggcta atttattaca acttataggt
     7441 tgaagctgta accaatttat agtcatatta tatttttatc atccagttta ttgaattctt
     7501 ctcgtttacc tcaattcatt ttcaagggga atcagctcat catctaacat tttacgccat
     7561 gctcgcttca tttcttgtgg gtatttaaat ggtttcgctt ctggatgttt ggctttatat
     7621 ccttcgtaat ccttactgtt gataattgct ttcatattag gaattggatc ttcacctggc
     7681 atatattgca gattagaagc atgtaatttt ttctcagaaa caaaccactc attggaaaag
     7741 acatgaatca aatcatcact tacttgtgta aaatagcgac gtttaatgac aaagatatct
     7801 tcattgctat ctagtgtacc ttcatttctt tgtgcttaaa ttcaaagata attttctcaa
     7861 taaagatttt tgattccagt aagattcctt tactcctttt acaacatagc gttgtccttt
     7921 gttttgaaac atcttcttcc cctctttgcc attaaaaata gcccaattct ctataaagaa
     7981 gattaagctt cattattata atataaagtt taaatttg
//
LOCUS       DARDHA010000126         7993 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000126 DARDHA010000000
VERSION     DARDHA010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.128"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            231..974
                     /locus_tag="SDA10_002176"
     CDS             231..974
                     /locus_tag="SDA10_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4241063.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            967..2547
                     /locus_tag="SDA10_002177"
     CDS             967..2547
                     /locus_tag="SDA10_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEG4241064.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(2798..3277)
                     /locus_tag="SDA10_002178"
     CDS             complement(2798..3277)
                     /locus_tag="SDA10_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEG4241065.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(3293..4045)
                     /locus_tag="SDA10_002179"
     CDS             complement(3293..4045)
                     /locus_tag="SDA10_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEG4241066.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            4162..4232
                     /locus_tag="SDA10_002180"
     tRNA            4162..4232
                     /locus_tag="SDA10_002180"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4194..4196,aa:Gly,seq:ccc)
     gene            4593..5318
                     /locus_tag="SDA10_002181"
     CDS             4593..5318
                     /locus_tag="SDA10_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEG4241067.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            5573..5839
                     /locus_tag="SDA10_002182"
     CDS             5573..5839
                     /locus_tag="SDA10_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEG4241068.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            5836..6267
                     /gene="ruvX"
                     /locus_tag="SDA10_002183"
     CDS             5836..6267
                     /gene="ruvX"
                     /locus_tag="SDA10_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEG4241069.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            6292..6597
                     /locus_tag="SDA10_002184"
     CDS             6292..6597
                     /locus_tag="SDA10_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEG4241070.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(6678..7823)
                     /locus_tag="SDA10_002185"
     CDS             complement(6678..7823)
                     /locus_tag="SDA10_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4241071.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
BASE COUNT         2525 a         1384 c         1559 g         2525 t
ORIGIN      
        1 tattgataaa ctaaagaatt ttatgcaaga aaagatagaa aatgaatcat tttaaagaaa
       61 acaaagactc atatcttacc tgttagaaga gtgttttcct gtagaagaga acaacccaag
      121 tagcagccta gtcgtttcta tccttttttg ttatgcactc tttttgacat ttactccacc
      181 aataacgaag ggtacaataa aaagtaataa caaacaaaag gagtacagaa atgattgaat
      241 tcatcaatgt aagtaaaagt tacgggaaaa aacaagcatt aaaagacttg tctctctcga
      301 taagacaagg ggaaattttt ggatttttag gacataatgg tgccggaaaa tcaactacga
      361 ttaaaagttt agttagtatt attgaaccat catcgggaac gattttagta gatggaatga
      421 aattgacgga aaatcgattg agtatcaaac aaaaaatcgg ttatgtgcct gattccccag
      481 atattttttt acaattgacg gctggagaat attgggactt gatttcagct gcctatgaat
      541 tagacacaca aaaaaaagaa aagcgtttag cagaattgac agcgcttttt gatatgtata
      601 gtcatcaaaa tgaaacaatc gcaagttttt cacatggaat gcgtcaaaaa acgattttga
      661 tcggcacgtt gctgccagat ccagatattt gggtattgga tgaacctttg caaggacttg
      721 atcctcaagc agcatttgat ttgaaagaaa tgatgaaggc tcatgccgca aaaggaaaaa
      781 cagtcatttt ttctacacat gtattggata ccgcacaaca gttatgcgat aaactggcta
      841 ttttgaagaa aggggaattg atttatcagg gggcagttag tgaattattg aatggctcac
      901 ctgatgaaac gctagaaaag atttatttga aaatggctgg aaggcaagcg acaggagatg
      961 gaccgtatgt ataagcgaca attattagaa ttaattcgag ttaatctttt atatgttaat
     1021 ccacagctca cgaataaagc aagagagaaa ggaaaaaagg gaaagcgatt aactatctat
     1081 cttttgtctc aaaatctttt ttcagggctg atttttttac tgatttacgg atttgctatg
     1141 tttgctatga actttgttaa attgccaggt ttctttactt attacgtggc actttttggt
     1201 atgttaggtt taactcaaag tatatcagtc atttataata tcttttttga aggaaatgac
     1261 ctgcaagtat ttttgcctct gccttttgga caagaacagg ttttttcagc aaagattttg
     1321 gtagttgctt tgaccatcat tccttttgtt tttccgcttc tagtcatgtt tattttaacc
     1381 ggatggcgag ctgatctagg gattttgctt actatcatta tctcaatcct tttgttttct
     1441 ctatttttga ttattctttt ttgtctggct agtttgattg tttttggttt ggctcgaaca
     1501 gcagtgttta aaaaacatag aaaaacagtt tctagtatct tgcttggcag ctcgatgctg
     1561 atcgcatttg ctggaatcat gtggatgaat catcaaacgg ggactattga aaatgagtta
     1621 acagatcggc atccgatttc gatattattg ccattctatg atgctgccag tcagccactt
     1681 tctcaacaag gtttattggg ttggggaata ctagctatca cggctctggg atttttcctt
     1741 gctgtaaaac agctgatagt accaaagctt acagagcaac tgactgattc aaacagtgag
     1801 ttcaaaataa aaagaaccca taaaaagaac caaaatgtgc atcagctttt atttagttac
     1861 aatgctcaat tagtaaaaga acctaatttg attatgcaag ttctttcttc ttccttgtta
     1921 actccaatca tttttattct tgcttttgcc ctaggaggcg aaataaaatt aactgattta
     1981 gataataagt taatcggtgt tgttttcctg gtagggatcg cattggcttt cttaacagtt
     2041 aatcagactt cttttatcag caatcttatt tctttggatc aagaaaattt tctgtttatc
     2101 cgttctttgc caatctctat gaaccaatat ttaaaagaaa agttcagatt cggatggata
     2161 ttgcagagtt ttttaactgg tgggatcgca ttattaagcg gactgtcttt tcagctgcct
     2221 ttgtttttta tccctagctt gctatttggt tctttattcg gctgctactt attgtcacta
     2281 cgttatttcg ctagagatta tcggttgctg ctgctgaact ggaccaatat taaccaatta
     2341 tttaacagag gttcaggaag tttgggttta gtcgcaacta tgatgggttc attgatcatc
     2401 agtattattc ttatcgtctt atacggaatg ttggctttgt tttatccatt ttggttagtg
     2461 aatattcctg tgatcggttt agttctaata ctttccgctc tatggatcgt ttattatcaa
     2521 cgaacgtttt ggaaaaaatt tgaataaaga agaggggccg tgacaaaaat catgtcgcag
     2581 cccctttttt acagatagag ggtgttcaaa agtcagtttc tcagtattaa gtcaatttta
     2641 tgtaaaaatg tgagaagcta tttttacaaa acttttttta atactcataa ttacttctta
     2701 ttgctcctgc ggttacttgt cgcaatgttt gtgagatatt ttgtcatcgc tcaaggctga
     2761 acgtctcttt cacaaccttt tctttgttac acttcgttta tttaaaaagc cgtttttttc
     2821 gtttcatcat gattgcgtca cgctttactt tgaaattacg gatgttatgg ttaaagactt
     2881 ctgataatac aagacctaaa gcgattgctc cagcaatcat aattgcttgg accgcataat
     2941 gagccgcttc tcgataggat tcagtaacga gatttcgaac cgcttgataa gctaaaccac
     3001 cagggactag aggaaccatc ccgggaatat tgaaaatcgt tacgggcatt ttcaaacgtt
     3061 tagagaaaag atcgctgatg aatgctactc ctaaagaacc aagcaatgaa ccaaaagcag
     3121 cagatccgcc tatttgaaca cagatccagt agatcatcca gccaaaagca ccagaaagac
     3181 cacaacaaat caatgaacgc ctaggtacat ttgtaataac ggcaaatgcc gcagtcgcta
     3241 aaaaactaaa agaaaactga ataaatatcg tccccatact tgtgttcctt tcctaataaa
     3301 aaagctggaa aataaaagca atcgcaaaac cgatcatcgt agcagtcatc atcgcttctg
     3361 ttccgcgaga aactcctgag acataatgtc cagctagcaa atcacggata gcgttcgtaa
     3421 tttgaactcc aggaactaaa ggcatcacac atccgataat gattagatcc tgattgcttc
     3481 ctaagccaaa acgtacgctg tagatagcgg ctatgccaat caataaagaa gataaaaatt
     3541 ccgataaaaa tttgattcta aaaaatttca aactatatag ataagctgcg tatcccaatc
     3601 ctccaatgac taaagtcaca ggtaaatcgg tcatctttcc gccaaataac accataattg
     3661 ttccactgat cactgccgcg ctaacgattt gcaaccagac aggaaagaaa gaacggtctc
     3721 ggtcgattgc ttgcagttcg cagtaaagtt cttctaatgt tagctctgaa gcaacatatt
     3781 tacgtgaaag atcatttact tttactactt tttctaaatt aatcgaacgg ttggtaattt
     3841 gttccattcg gatggtcgag gtacgatcaa gaccaataaa gagaccggtt tgagtgacat
     3901 agctgaccaa acggaaattt ccagaagcta aagcaattcg actcatcgta tcttctaccc
     3961 gatacatctc cgcattgctt tccatcatga ttttcccagc gagtaaacaa gtgtccagca
     4021 acagatctaa ttcttttccc gtcatagcat cttctccaat aattgattga ttagttttat
     4081 tttactacaa aacataccag aagcgtaaag aataaaaaaa cttgaggaag gaataaaaga
     4141 ggcgtatact gtttttgtat tgcgggtgta gtttagtggt aaaatcccag cttcccaagc
     4201 tgatgtcgcg ggttcgattc ccgtcacccg cttaaaatag gttgtgaaaa agctgctctg
     4261 caacaagtaa taagaacagc caagcaaaaa caacttctta tgtttttaac ttggatggac
     4321 ttattaccgc agatgcaagc ttttgcgcac cgtttaccaa gcggttgtga aatcagtgta
     4381 ttgtcttgag tatgaagaaa atttttgaat aataataaat accttacaga tagagtggtg
     4441 actgattaga gacgaggaat ccgtctcttt tttgtatttt taagccatct taaaacaggt
     4501 tttatttttt gctgtttcta tttctttcta gtatttattt ataggggaac atatgttaag
     4561 atttagggag ctgttagtaa aggagtaaag aaatgagcaa aaaaacaacc aaggcgatcg
     4621 tggggatcgt cgctgctgtg attgcagggg cattcggtgt ttccaccaca caaaaagatt
     4681 cagatatctt tcagcagatt acaactattt ttgattccac accacaagct tctcaagtag
     4741 cggatgtgcc agaatatgat ggaaaacatc aagaaataga aatcaatggg aatcagccgg
     4801 aatttacaaa agaggatctt agcctttcca aaggaacatg ggaaagctat tcaaatattg
     4861 ataaattgaa cagagtagga caagccaatg ctatgcttgg aaaagatttg ttccctactg
     4921 aaaagagaga gcctttgtat attgacccga caggttggaa acaaaagaaa ttaagtgacg
     4981 ggcaatggtt atataatcgc agccatttaa ttggtttcca gctgactggt caaaataaca
     5041 acattaaaaa cttgatgaca gggacacgtt cgttgaatgc tccatacatg cttgctcacg
     5101 aaaatgatat tgcggcatat atcaaagaaa caaatcatca tgtacgctac cgtgtcactc
     5161 ctcattttga ggggaatgaa ctggtagcca ggggagtgca attggaagca gaaagcattg
     5221 aagacaaaaa aattgaattc aatgtcttta tctacaatgt tcaagatggc tacacgatca
     5281 attatgagac aggacaagca acaaaaacaa aaaattagta gcagctattc gaatttttta
     5341 tgaatattat gaaaaaattg tgtaaaaaca tacattcaaa gaaatatagc gtatacttta
     5401 tgaaaagagt acttttgata aagctacaga aaaaaggaaa aaaacataca aatagtgttt
     5461 cttaatttat tcggaagttt ggaatatttg tttgtagttt tccctttctt cgagtaaaat
     5521 aaggttgtag aaggtaagct ggaattacct tgcgggaaag aggtgtagaa tcatgggttt
     5581 tacagatgaa accgtacgtt ttggttttga cgatagtcgc aaaaaagaag tgagcgaaac
     5641 attagcgatt gtttatcggg ctttggaaga aaaaggctac aatccaatca atcagattgt
     5701 cggctacttg ctttctggag acccggccta cattcctcgt tatcgtgatg ctcgaaattt
     5761 gattcgccgt cacgaacgcg atgagatcat ggaagtaatt gtcaaggact acctctcaaa
     5821 tcatggagtg aatttatgag agtaatgggc ttggatgtcg gttctaagac agtaggtgtt
     5881 gctatcagtg acccctttgg ctggacagct caaggagtag agatcattcg gatcaatgaa
     5941 gcagaagaag agtttggctt tgatcggttg aaggaattag tagcacaata tgaagtagac
     6001 cggtttgtag ttggattacc taaaaatatg aacaattcca tcggaccaag ggcagaagct
     6061 tccatgtcat acggagaaaa gatcaaagaa ctattctcct tgccagttga ttatcaagat
     6121 gaacgattaa ccactgttca ggcagaacgt atgctagtag agcaggcaaa tacttcaaga
     6181 gccaaacgaa aaaaagtcat cgataaatta gcagctgtga tgatcttaca aaattattta
     6241 gacggctcag gtaagcaatt attttaataa taaaagaaaa gaggtaatct tatgacagaa
     6301 cataaccatt cccacgacca tgaaggacac gaacacatca cattggtgga tgaccaagga
     6361 aatgaaacat tatatgaaat cctactaaca gttgacggac aagaagaatt tggacgtaac
     6421 tatgttcttt tatatcctgc tggtgtttca gaagatgaag atgtagaatt acaagcttat
     6481 gcatacatcg aaaacgaaga tggaacagaa ggcgagttag aacaaatcga aacagacgca
     6541 gaatgggaca tgattgaaga agtattcaat acattcatgg cggaagaaga agaatagact
     6601 ataaagctag gagaaacctt gatatgatag ggtttctcct ttttttattt tttactgtat
     6661 gccatattgt atgccatcta gtcaatattt aagtgttgta ctaatttttc aacagtcttt
     6721 tctttcgttt cttttgccaa gtgaccgtaa caatcgatcg tctgactaat ttttgtatgc
     6781 cgtaatctag tttgaacttc taccatagta gctccagcgt ccaagagcat tgaagcatga
     6841 gtgtgtcttg ctacatgcac accgagaaat ggaaccatag ctttttcaca aatagtaatc
     6901 aatcttgcat aaatagcgtg gtgtgtgatt agttctttat agataccaca aaaaatcata
     6961 gtcggatcag ttacaccaag tgtcagcata tatttccgct gattaatctg ccattttttt
     7021 aatatagaaa ttgttctttc atctagtgga agaaccccag cactacaatc agttttagga
     7081 tcactgattt tatagccatt tttggattgg cttagtgttt tattgatggt gaccgtattt
     7141 tcttgaaaat caatgtctga ccaattcaat gcaagggctt cactgatcct aaccccgcta
     7201 taaaagagaa agcgtagtaa cgttttatca aagtcgtttc gataagtttc aggtttgctg
     7261 tttaggtaat caaacaaggt gatcagttgt tctttactgt aaactttgac cttttcttta
     7321 tgcttagtgg tggttctttt aggcattaat acgttgtcta gtggattagt ctcaataatc
     7381 ccaagattga tagcgtagct aaaaatacgc ttagaagttg aatgaagtac cttgtattgg
     7441 tcagtagttt cagcccagct atttacaatt tgttgggctt tttttaccgt gacttcattc
     7501 acacgaagat ttccatattt tggaagaatc cactttctaa acttgatttc agttttcatg
     7561 aaggtggctt ctcggacagt ggttttatat gtttctagcc acaagtcata aacttcttta
     7621 aacgtttcgt tgttcgcttt tttaataccg tttttatcgt agtccacttg caagcgtgac
     7681 aaggcaagct gggcttcttt ttttgttttg taacctcgtc tacgaacctt atcaggtttg
     7741 ccagtcattt tgttcattcc aagataggct tcatagagcc aaaatttacc ttttttagtt
     7801 tcatattgct tgaatgttgc cataatagtt ttccctccat agtgtagcaa gccgtggggg
     7861 cggttggagg aggcagttta ttattatttt gaatagtgtt tatttagctc agtaacaaga
     7921 gctgtgaact cttttagaag atcattttca taaaattctt gatactcttt tttctcatct
     7981 gtattttttt ctg
//
LOCUS       DARDHA010000127         7960 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000127 DARDHA010000000
VERSION     DARDHA010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.129"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            173..874
                     /locus_tag="SDA10_002186"
     CDS             173..874
                     /locus_tag="SDA10_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG4241072.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1483..2499)
                     /gene="tsaD"
                     /locus_tag="SDA10_002187"
     CDS             complement(1483..2499)
                     /gene="tsaD"
                     /locus_tag="SDA10_002187"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEG4241073.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2496..2960)
                     /gene="rimI"
                     /locus_tag="SDA10_002188"
     CDS             complement(2496..2960)
                     /gene="rimI"
                     /locus_tag="SDA10_002188"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEG4241074.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2967..3509)
                     /gene="rimI"
                     /locus_tag="SDA10_002189"
     CDS             complement(2967..3509)
                     /gene="rimI"
                     /locus_tag="SDA10_002189"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEG4241075.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3493..4215)
                     /gene="tsaB"
                     /locus_tag="SDA10_002190"
     CDS             complement(3493..4215)
                     /gene="tsaB"
                     /locus_tag="SDA10_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEG4241076.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4406..5386)
                     /locus_tag="SDA10_002191"
     CDS             complement(4406..5386)
                     /locus_tag="SDA10_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4241077.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5410..6894)
                     /gene="galT"
                     /locus_tag="SDA10_002192"
     CDS             complement(5410..6894)
                     /gene="galT"
                     /locus_tag="SDA10_002192"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEG4241078.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6906..7895)
                     /gene="galE"
                     /locus_tag="SDA10_002193"
     CDS             complement(6906..7895)
                     /gene="galE"
                     /locus_tag="SDA10_002193"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEG4241079.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2241 a         1707 c         1401 g         2611 t
ORIGIN      
        1 aatcattcgc ttcaaaacaa attattcaat accattgtat attttttaca taataacaaa
       61 ttatatttgt aaagtattaa aacttgtttc taatgttggt atgttaaaac tttacaaatt
      121 ctttaaaaaa agtgacaata aatatagtga acaataagga gaggactttt taatgcgtgt
      181 taataaaatc gattttcact tttcaaaact cagatatcaa cctgattttg aacatttctc
      241 ggatgaagag ttccaacaga tcaaggaaca tactgttctt cgttcttata aaaaaggaca
      301 gatccttttc gacgatggtg atcccagaca acggttgtat atcttgacca aaggactcgt
      361 tcgtttagaa cgttatgatg aaagtgcaac ttattactac tatgactatg tgaatacaaa
      421 taaccttttt cctatgtcag gaatgtttgg agaagagacc tacacttata cagctcaagc
      481 gatgactgat attgaaacat tctaccttcc tgtttcctac tatgaaaaat tagcacgaaa
      541 aaatcccgaa cagttgatct atatgataca aaaaatttct aaagtagttg aaatacatga
      601 attgcgtatc caaatcggat tgacctctag cgcctttgac cgggtaaaac aaaatttatt
      661 cattttgaaa gaagaattgg gcgtaccaac aaaagatggt catatcacca ttccttatcc
      721 gatcacctta aaagaattag cagtgaatag cggcacgaca cgtgaaactg ctggacaagt
      781 aatcaaacaa ctacgtgatt ccgggctgtt agattatgac aaaaagcgtt ttactttctg
      841 taagacagct actactccag ctatgagaga ataacgcgaa caaaaataag cagtgaccct
      901 gataaatatc agggtcactg cttattttcc tccctaaaac ttctgaaaga tccggcagtt
      961 ttagatcact ggacaagact caaagatttt ctatgtgtgt ttcagttatg taatattcac
     1021 tgtttgcaaa aaatactgtt agattaacta gatgaagcgt cttgtcctca tccgatctag
     1081 attattcgat attcctcgac aataaacgat tactcagtga gggcaatcag aggaacggat
     1141 aatgataaat aagctgtaaa aaatagcaaa ataacaataa tgattaaaag tatgtaaaaa
     1201 atagggcttt gacaaaaacg tgtcacagcc ctatttcgat taaacggtgt gtacctgtca
     1261 actcttcggt cacaagtcat aatgttcata aacatgagga gctgtttttt gaaattgttc
     1321 cttgtgaatc agagttaaac gacaggctta cgaactcttg attaaacggc gttcaaaagc
     1381 ttgcatacgc ggtaataaga acagccaggt caaaaacatg agaagccgtt tttcctgggc
     1441 tgttcttatt acttgatgca gggcagcttt ttcacaacct ctttattcag ataaaaccaa
     1501 gcttggatca gcattcaact gatagctagc aaaatttctt ttttgcattt ccacatgtgc
     1561 tgctgctcca atcattgcag cattatctcc acaaagtcga agagggggaa tcactagttc
     1621 tacctcaggg agtttagctg ccaaagcatc ctgcaatcct tcacgcaacc cttgattggc
     1681 cgctacacct ccagctataa tcagttgctt gactggataa ttttgacaag ctcgaagtgt
     1741 tttagtaatc aatacatcga tcacacttgc ttgaaagcta gctgctaaat catttttgtc
     1801 tagatcttct ccacgctgtt gtgcattatg caccaaattg ataaatgcac tttttaatcc
     1861 gctaaaactg aaatcataat tatcttcatg gatcatcgct cgcggaaaat ggtaattatc
     1921 ttttccctga tgagctagct gatcgatttc ttttccgctt gggtaagaaa gacctaatac
     1981 tcttccaact ttgtcataag cttctcctgc tgcgtcatct cttgtctccc caatgatttc
     2041 atatgatcca tcttcttgca tatagactaa ttctgtatgt cctccgctaa caagaagtgc
     2101 catcaatgga aactggaatg gtttaaccag acgggctgca tagatatgcc ctgccatatg
     2161 gtttacagga ataagcggca attggtgtgc ccaagcaaaa gccttagctg cagaaatacc
     2221 aatcagcaac gagccgacaa gtcctggtcc ataagtgaca gcgactgccg ataaatcttc
     2281 tgccgacaca ccagcttcta ccagtgcatc ttccaagcat aaggtgattt gttctacgtg
     2341 atggcggcta gctacttcag gtactacgcc accaaaacgt ttatgactgt ttatttgaga
     2401 agcaacgatg ttagataaga tctctgttcc atttctaacg accgctacac tcgtttcatc
     2461 acaacttgat tcaatcgcca gaatcaattc ttcattcatt tcatcgtctc cgtttttaac
     2521 tttctgctca taatgatcgc atcttccgtc ggattgcgat aatatgattt ccgtttacct
     2581 aatatacgaa acttttctga ttcataaagt tttcttgctg cttcattaga tagacgtact
     2641 tctaaaaaaa catgcagcga atcttttatt tgttccgtaa tcagtaattg taacaaatgc
     2701 cgtgcatgcc cttttccttg ttctgaaact gcaacagcaa tatttgtgat ctcgacttca
     2761 tctattactt tcgagtatcc aataaagcca cagatctggt tatcttcagc taaaagcaga
     2821 taatcagagt gaggctgttg gatgtctgcc aaaaactgca ttttcgtcca aggtgaacca
     2881 tgtggataag cattttcgct aatcatccat acgcattctg ccaagtacgc ttcatcaaaa
     2941 tctttttttc tgcaaatcaa ctaatctcac tcattttcta aaagttttat catatccaac
     3001 gcatcttctt ttgtttcagt gtaatatctt tttttgatcg ttgttgcttg aaaccccaat
     3061 ttccgataaa gtctttgtgc atcttgatta ctcatacgca cttctaatga catcgtctca
     3121 cagcgattca ttatagcaaa gttctcgatt tcgtcaataa gcagcgaacc gattcgttta
     3181 cgctgatatg ccggtcgaac ggcaatattc gtgatatggc aatctaaacc gaacgctctt
     3241 gaaccgataa aaccaatcat tcttccatta tcaaagatac ctagatatag atgaggaagc
     3301 ggtgacttca attctgatat aaaggcactt tttgtccatg gcaattcgcc attatagact
     3361 tctcgttcga catccagcag ctctttgata tcttctgtag tcaattctct tagtacgtaa
     3421 cgacgatcga tttgttcgac tgttttttct gtataaatca ttttatcttg aaaaaagctt
     3481 ctggcatacg ctctaaattt tttcaacata actttcgtct ccaggtgtgt gcgtctccaa
     3541 ccatttttct tctgcttcta ctcgttttaa atagcgagga agaaaagctt gtttctctgc
     3601 aactggttca actaaagcac ctaattgagc cacagttaca ccacttggaa tatcccatgc
     3661 tggaactgta ttaatcatcg cactaggcaa tatttccttt atttcagaga agaatttgtg
     3721 acaatcactc ccgacgaaat aaatagcttc attcagcttt tgtagttgtg tgaacaaatc
     3781 agaagcagaa atatgtacat cttcaagtac agtttctaat ctattttctt tccagcggta
     3841 tgccccagcg tatacatttt ttcttcgagc atcaaacatg ggcacaataa tattatccac
     3901 accgatgcaa ttagccgcga tggttttcag actagatact cctactagct ctttatctaa
     3961 tgtataagca atcgtttttg ctgtcgttac acctatacga agccctgtat aagaaccggg
     4021 accatcagaa accgcgattc ggtcaatgtc ttttggactt atttgaagat ctgcaaacaa
     4081 ttggtcgatt gcaggcatca gtgttacact gtggttgcgt ttgatatttg tttgtatttg
     4141 tccaagaatt tgttcgtctt ctaccacacc gatcgccaaa gtttggtttg cagtatctat
     4201 tcctaatgta atcatatatt tgatcctttc tgctatgaca ttctcgcctt ccattgtagc
     4261 atactttttt attctgagga tgaaaatacc aaaggcaaaa ctagtacaat aatacatgat
     4321 tatctgctgg cgggcaaatt ttgccccttc ctgaaacaca aaaaagctgc ggtatcactc
     4381 cattttccca cagcttttcc atcttttatt tcgttgattc tcgaataatc aactctgttt
     4441 ccacaataat ctttcttgaa accggtgctt tggaattact taattgaaga atcgtctcca
     4501 ctgctagctc tcccatccat tcggtataaa cttttacagt agaaagcgag ggcgttacat
     4561 atttcgctac actgacatca ttgaagccca tcacagaaat atcttctggt accactaaac
     4621 ctttttcttg aatcgctttc agtgcgccaa tcgctaatgc atcattagct gcaaaaagcg
     4681 catcaggaac atgcggattc gtttttaaat attgcttcac tgcttgatat cctgactcga
     4741 cagaaaaatc tgcttcgata attggacctt cgtcgtagcc tttttctgcc aaaacttgtt
     4801 gaaatgccat aattctggga tcttttagcc gctgatggtt tttcttcgta tattctgttc
     4861 cagcaagcat agcgatatgt tgcgctttat gggagacaaa gtgttcaatg actttttgaa
     4921 cacttgactt aaagtcgaca actagagagt cgattccttg agaagtgccg tcagaatcta
     4981 ccaaaagcaa ggcattttgg gatcctttca tttctaataa ctgagcttcg tcgaattttc
     5041 ctagtgcgat cgttccatct gccggaagat cactaatgga atcccacgtt tctttcaata
     5101 aagtaacgtt tgcttcttct gccttttttt cgattcctag gcgaatagat aaataataaa
     5161 gatcttcaag ctcttccgta tcgtcgtacc actggatcag gcgaagcgtt tgcttagtat
     5221 ttgctgcatt tttacgatgt ttcgtataat ttaagcgttc tgctgcttca aaaatttttt
     5281 gctttgtggc taagccaacg gaaagatcag gatcatagtt gagaactctt gaaacagtag
     5341 caggagaaac tcctgccaac tgcgcaatgt ctttaattgt tgccatttgt ctctcctcct
     5401 tttttacgtt tataaaatcg cagtaaatcg ttcaaacgct gcctgtcctt tttcatctcg
     5461 cttgaatact ccagcgtctt ccagtacacg tgcaaaaacc tgacctacag atttttgtac
     5521 gacatcggtg accgtatctt tcgtcaaatc aggatgttct ttttttaatt gttctgccca
     5581 cggacgatga tattcagcca tctggttttc ttctcctaat aagaaatgct ctacttctac
     5641 tagttctggt ttcaaacgag gtggtaaaac agcaagcccc attacttcga tcaacccaat
     5701 gttctccttt ttgatatgtt ggacatcttt atgtggatga aaaatcccat caggatgttc
     5761 ttctgaaaca ttgttgtctc gaaggacaag gtccaactca aagtaattgt cccttcttcg
     5821 tgcaattggc gtaatcgtat ggtgtggtgt accatctgct gaaaaagctc tgatagatac
     5881 tgtttcatcc gaatatgctt tccatttttc caatactgag ttagctgctt caataagcaa
     5941 gtttttctct gttgattgca aacggatcac agacatcggc catttcacga ctcctgcgat
     6001 gacttgcgga taatcttcta gtttgataag tttttctatc ggtgcttttg ccataggaaa
     6061 ttcatgacgt cctgcctgat aatgatcatg agaaagaatc gatcctccaa caattggtaa
     6121 gtcagcattc gaaccaacaa agtaatgagg caagacttca gtaatccgaa gcagtctagc
     6181 aaatgtttcc tttgtgattt tcatcgggcg gtgttgttct gataaaatga tcgaatgttc
     6241 attgtaataa gcataagggg aatattggaa tccccagctt tcaccgtcta gattcattcg
     6301 aataatccgg tgattggtgc gggctggata gttcaagcga cctctatatc cttcgttttc
     6361 catgcaaagc atgcaggtag gatagtctac ttttaccgcc tcgcgttctg ctgcgatttg
     6421 ttttggatct ttttctggct tagataaatt gattgtgatt tccagttcac catactccga
     6481 ctctgccgga aatttgatgt tttttgcgat ggctcttgtt ttgatgtagt catttttctt
     6541 acttaaatta aagaaatact cagttgcttc ctctgggctt ttcgaatagt gctgtgcaaa
     6601 gaatgcattg acgactgaag gtggcggtgt gagaaaatcc attaattgcg cttcaaaaat
     6661 ctcttgttct gctggcaatt ctgaaagaac accattttct cttgccttag cgacaagctg
     6721 atccaataag tctacagatt cttccttaac tggtcttact tcaatgcttt ctagagcatc
     6781 ttctcctacc atccctaaaa gacgattctg taaatataag cggtccattt ccatccatcc
     6841 gccagcttcg atcgcaagtg tggcaaaatc acaaatcgtt tgactgattg acatatctca
     6901 ttcccctatt cttcataacc atttggatgg ctgacatgcc aatcccatgc tgttttgata
     6961 atcgctttga tatctgtgta ttcaggtttc cagcccagaa tctctctcgc tttttcactt
     7021 gaagcgacta atcggctagg atctccagct cttcttggtg ctatttctgc tggaatttct
     7081 tttcctgtca cttctctagc tgcttctagc atttccttga ctgagtatcc tttgctgctg
     7141 cctaaattga agaagttgct ttcatttcct tctttcaagt attccaaagc taaaatatgt
     7201 gcagcaatca aatcttctac ttgaacataa tctctgatac atgttccatc tggtgtatca
     7261 taatcgtctc cataaacagc caaggctttt ctttgaccaa gagctacttg taaaatgatt
     7321 gggacaagat gcgtttctgg tgtatgatct tctccgatag atgcatctgc ttttgctccg
     7381 gctacattga agtagcgcaa agcgacatag cgcatgccat atgcttggtc acaccacttc
     7441 atcatttttt ccatcatcaa tttactttca ccatacggat ttttaggatt agctggtgta
     7501 tcttctgtga ttggagactc tttcggctct ccataagttg ctgcagtaga agagaatacg
     7561 attttattca cgttgaactc gtgcatgact tcaagtaaga tttgcatacc gtatacgttg
     7621 ttgttgaaat acatcaatgg tttttcgaca gattctccta ctaaagagct tgcagcaaaa
     7681 tggattactc cttcaatcgg ttccttttca aatacggaac gtagaaattc tttatcccgg
     7741 atgtctcctt cataaaaatg agcatctgga tgtactgctt gtttgtgacc tgtgagcaaa
     7801 ttatctacca cagctacttg ataccccttt tgaactagct ggtctacagc gtgcgaaccg
     7861 atataaccgg ctccgcctag tactaaaatt gtcataaaaa acactccttt gattagtaaa
     7921 attgtgtttg ttgattctat ataatttaaa aaacgaagtt
//
LOCUS       DARDHA010000128         7868 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000128 DARDHA010000000
VERSION     DARDHA010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.130"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            243..905
                     /locus_tag="SDA10_002194"
     CDS             243..905
                     /locus_tag="SDA10_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG4241080.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            1140..1283
                     /locus_tag="SDA10_002195"
                     /pseudo
     CDS             1140..1283
                     /locus_tag="SDA10_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            1433..1633
                     /locus_tag="SDA10_002196"
     CDS             1433..1633
                     /locus_tag="SDA10_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG4241081.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(1887..3566)
                     /locus_tag="SDA10_002197"
     CDS             complement(1887..3566)
                     /locus_tag="SDA10_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEG4241082.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(3568..3780)
                     /locus_tag="SDA10_002198"
     CDS             complement(3568..3780)
                     /locus_tag="SDA10_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEG4241083.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            4173..5903
                     /gene="efrA"
                     /locus_tag="SDA10_002199"
     CDS             4173..5903
                     /gene="efrA"
                     /locus_tag="SDA10_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HEG4241084.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            5900..7660
                     /gene="efrB"
                     /locus_tag="SDA10_002200"
     CDS             5900..7660
                     /gene="efrB"
                     /locus_tag="SDA10_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HEG4241085.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
BASE COUNT         2436 a         1341 c         1648 g         2443 t
ORIGIN      
        1 ttgcacttga cacttcagat ggacgttttc ttcaaaataa tatgttttga aacttgataa
       61 gagaagccac tgtctttact aaaaagtaaa ggctgcgata aaggacagtg actttaataa
      121 agttgcactg ttgtattgtc tttggataaa attgtctaaa gggaagtttt atccccaacg
      181 aaaaaacatc taaagtggta agtaatgaaa gaatgtcgat aaagtaggaa tgggggatct
      241 ttttgaatac aatagaatca cagtttgata aggttgcaga agattacgat ttcgtgaatg
      301 agcttttgaa tgattattca ttctttgtgt ctaatatgtc tccaaagaaa ggcagagcat
      361 tagatatcgg atgtggctcg ggtttgttag tggagaaatt agcaagttat tatgatgaag
      421 tggtagggat tgatatttct aatcaaatgc tcgatcttgc caaatctaaa cgtcaactaa
      481 caaatacggt ctatctgaat atgaatgcag aacaacttaa ttttaatgag aagtttgatt
      541 ttattgtaag ccgaacaacc tttcatcatt tggatgatat agccagcgta atacagcaaa
      601 tgaaggaact gttgaatgag gaaggaagaa tagttattct tgataatgta tctgaagtcg
      661 agacaccgcc tacctatgtt tataaattag gggcaatcca agagttttta ccgcattgtt
      721 tcaaatttgg aataaaaaat gcgattagaa tttataatca caacacatct aaatcatggc
      781 ttgagcattt agcttcagat aagtacctat ctgaacaaaa ttattacgat ttatatgaaa
      841 agttgttgcc tggatgccaa ttccataaaa tgggttgggc aatgggggtt gtctggacaa
      901 aataacttaa agctgtggct ttgaaaagtt gaatttgtga ttgttttcag agctatcggg
      961 tgctttagta taaataacaa tgaataaaaa atcagcctgt atcttatgtt actattaagt
     1021 aatttaagat acaggtttta ctatttttga aataagtacc ttccacttca aatacatgta
     1081 ttcaaagtga aagtattgat ttaaatgatt cttttgtaat ttactcttga taatatgaaa
     1141 ttccatcgca gcaggatatg aattttacca aaaggataca aaagtgtgga gaaatgatgg
     1201 ggatcactgt actggaccac ttgatcatcg gacgcaaacg atattttagt ttgagagaag
     1261 aaggaatgat ggaagagaag taagttgctt tcttgcaaaa aaaatgaagc aagtgtacaa
     1321 ttatattgta attttgtttg ggaatagaag ttgaaacatc tgttgcacct ctatcgtaca
     1381 cgagcaatgt tacaatttaa attagtgctt ttggcacgag gaggatatta ttatggaaaa
     1441 cggaacagta aaatggttca acgctgaaaa aggatttgga tttatctcac gtgaagatgg
     1501 aagcgacgta ttcgttcact tctcagctat ccaaggtgac ggattcaaaa cattagaaga
     1561 aggtcaagca gtgactttcg acgttgaaga atcagaccgc ggcccacaag ctgcaaacgt
     1621 tgtaaaagct taattttatc tgtaaatcaa attgatggta gacatcaatt tttacgagag
     1681 aacaaagcga gtaacaaaaa tacctgccac aagagacaat ttctcttgtg gcaggtattt
     1741 ttttgtatga aaaagtaaat aaatttaaga ggctatgaaa gagccacctt gtatcaagga
     1801 ataagaacaa acaaaaatgg cttctcatgt ttttcatttg ttttctctta tattcaggac
     1861 aaggtgctga tttcataacc tcttatttat tcttcatctg gtgtcattac cattggcaaa
     1921 atcattggat gtctttctgt acgttcgaat aagaaaggct gaagcgcgcc agtcatcgct
     1981 tctttcaatt tatattctga acagtcaggt ccattcaaac attctcttaa agcgtggaat
     2041 aagattcgct gagcttcatt gatcatttct cctgattctc gcatgtagac aaatccacga
     2101 gagagaatat ctggaccagc caagatttct tttttcttga tatccactgt cgcgacagct
     2161 aatacaagac cttcttctga aagcaggtga cggtcacgta aaacgacatt accaatatca
     2221 ccgatcccgc taccatcgat atacacatct ccagccacaa aatgcccagc aggacgcgca
     2281 gaatcgctag ttaacgctaa aacatcgcca ttttccatga tgaagcagtt ttctgcagga
     2341 acacctacat cttgtgctag agaagcatgt actttcaaca tgcggaattc tccgtggaca
     2401 ggaataaaat attttggctt catcaaacga agcattaatt tttgttcttc ttgtccaccg
     2461 tgaccggaag tatggatgtt attgattttt ccgtgaatca cttctgcacc agcttcagac
     2521 agcaaattga tcaaatgatt gacacttgtt gtattaccag gaataggtga acttgaaaag
     2581 atcactgtat cgtctggctg gatttggatt tgacggtgag taccattcgc gatgcggctc
     2641 aacgcggcca tcgcttctcc ttgagaacct gtacatagga tcatcgtttc attggcaggc
     2701 aagcgattga tttcgtgtgc atctacaaat gtgccgtctg gaactttgat atatcctaaa
     2761 cgttgaccgt tgacgatggc attttccatg cttcgtccga aaacagcgat tttacgacca
     2821 gttgccacag cggcatctgc tgcttgctgc aaacggaaga tgttagaagc aaagctggca
     2881 aaaatgatac gtccttcgat ttttgaaaag atcttcaaaa tagaatgccc aatcgttttt
     2941 tctgatttag taaatgttgg aatctcagca tttgtactat cagataaaag gcacaagact
     3001 ccttcttccc cgatacgagc cattttatgc aagtttgctg gttcgccaac aggagtgaaa
     3061 tcgaatttga agtctccagt cgctacgata tttccagaag gtgttttcac cacgatccct
     3121 aatgcatctg ggatactatg ggtcgttctg aaaaagctga cagaagtctt tctaaaacgg
     3181 atgatcgtat cttcattgat ctcatggagc tctgcatctc gaagcaagcc atgctcatct
     3241 aatttgtttg tgatcaacgc taatgcgaga ggaccagcat agataggaat atttgcttgt
     3301 ttcagcaagt agggtattcc gccgatgtgg tcttcgtgcc cgtgagtaat caccaaggct
     3361 tttactttgt gtaagttttg tatgatgtag ctgtagtcgg gaatgacata gtcaattcca
     3421 agtaaatcgt cttccgggaa tttgatcccg gcatcaatca aaatgatttc atcttggaat
     3481 tgtaccccat acgtattttt cccgatttca cctaatccac caatagcaaa gacgcctgtc
     3541 tcgttatttt tgatggaaag tttcatatta aaactccact aatgagaaat cagcattttc
     3601 tttttcgtac tctagatgac ttccttctag aagttgaacg aattcaatat tgtatggggt
     3661 attttcttcc acacgttggc gagcgtctac atcgcttttt gcctcaatgt aaagagattt
     3721 tgtttcttcc cgtttagggt tgcgtacttt tgtttcttga taataaactt tatagatcat
     3781 tgttttgtct cctttatctg cgtaaagcag tgatttagct agttgatttt tttagacaac
     3841 acaaaacgag gggacaagcc acctggtttt tcatatgctt tgactagaat actggattcg
     3901 agtcaaagag acaagcctcg tttttgctta tctgttcgga tattttttca accgtacctt
     3961 ctgtataaaa aatatacaac aattaattgt tattctagca taaaacagca aataagtata
     4021 gaaatcaact agattgaaag aaaaaatcaa tgtttatcat cccgctataa tcataatgac
     4081 ttttttatga gagaagtgtt ctattaatag gctcgttgtg ctatcatttt tagtaatgaa
     4141 aatgtaacaa aagattaaaa ggagatgaga aagtgaaact aatgtggcat tataccatgc
     4201 gttataagaa attcttgttt ttcaatttca tatgcgtatt tggttttatt ttgattgaat
     4261 taggacttcc tacgatttta gcacgtatga tcgatgtagg aatccgcaat gatgattttg
     4321 attatgtgaa gcaacaaggg atatttatga tcgttattac tgtaatcggt attattttga
     4381 atatcttgtt aggttatttt ggttcgagga ttacgactaa tatcgtagca gatattcgag
     4441 atgatttgtt cgaaaagatc caaagttatt cgcatcagga atacgaaaat ctaggcgtat
     4501 cttcattgat cactcgtaca acaaacgatg cttatcagat catgttgttc ctgcaaaata
     4561 ttttacgtat cggttttatg gcaccaatga tgttcattgt cagtttgtac atggtcatgc
     4621 gtacaagtgt gacactcagt ttgtatgtag taggtgcatt acctttacta ttattggcag
     4681 tcgtagcgat tgctaaggtg tctgaaccgt tatctaaaaa gcaacaaaaa aacttagata
     4741 aaatcaacag tatcttgcgg gaaaatcttt caggattgcg tgtgattcgt gcattcgtca
     4801 atgaaaagtt tgaagaaaaa cgttttgctg tagtcaatga gaattatgct tctagttcaa
     4861 aaagcttatt ccgtttgatg gctgttgcac aaccgggttt ttatttctta tttaatatcg
     4921 taatggtatt gatcatctgg agcggggcag tgcaaatcga tgcaggcgag cttcgtgtag
     4981 gagatttgat tgcgtttatc gagtatatat tccatgcctt gttctcattc atgttatttg
     5041 ctagtgtgtt catgatgtat ccaagagcag ctgtttcagc tggacgtatc caagaagccc
     5101 tagatgccaa accttcgatt gtagaagatc caaatggtat atctgataca aaaactaaag
     5161 ggtatatcga atttcaaaat gtaacatttg cttatccagg ccattcgcaa gagccagtga
     5221 tacgaaatgt cagttttaca gcctctccag gagagaccgt tgcttttatt ggaagtaccg
     5281 gtagcggaaa atctacgttg atccaattga tccctcgctt ttatgatgtg agtgaaggaa
     5341 agatcttgat cgatggagta gacgtgagag attatcggct aagcaagttg cgagataaga
     5401 ttgggtttat tccgcaaaaa gcgctgctat ttactggaac gattgcagat aatttgcgat
     5461 acgggaaaga agatgctacg caagaagaaa tggaacgtgc agctgagatt gctcaagctt
     5521 cagaattcat ttctcgaaaa ccagatggat ttaatgaaca tttatcagaa ggtggttcca
     5581 atttttcagg tggacaaaaa caacgattgg caatagccag agccatcata cgccgtccgg
     5641 aaatctatat ttttgatgac agcttttctg cattagatta tcaaacggat gcgaagttac
     5701 gtgctcgtct gaaaaaagaa acgacagaat ctactgtatt gatcgtagca caacgtgtgg
     5761 gaacgatcat gcatgcagat aagatcatcg tattaaatga aggagaagtc gtcggtatgg
     5821 ggacgcatcg ggagttgttg gaaaaatgcc cgatttatta tgatattgcc gcttctcaat
     5881 tatcaaagga ggaattagca tgaaaaaaac cttttcttcc ttaaaacgtt tagctcgata
     5941 tatcagaccg tatcgtgaga cttttctttt agtgatccta tttacagttc tgacagtggc
     6001 tttcaacgtg gctttgccat atgttacagg acttcctact acagaaatca gccgtaatat
     6061 tgccaatggc gaatcaatca atttttctta tatattcaaa tgtctcattt ggatcacagt
     6121 tgttggaata gggtattgta tttcgcaatt actttctggt gttttgatga caaatgtcgt
     6181 tcaaagtgcc atgcatgatt tgagaagaga catagaagaa aaaatcaatc gtcttccggt
     6241 ttcatatttc gataaaaacc agcaaggaaa tattttgtct cgtgtgacga atgatgtaga
     6301 tgcagtcagc ggtgctttac aacaagcttt tattggtatc gtaaatgcga tattgggaat
     6361 tacgttggct gcagcaatga tgttttatat ccagccgatg atggcgctga tctctatgat
     6421 catgattcca acatcgatct ggatctcaaa acgagtgatc aatgcttcgc agaaatattt
     6481 tcaaagcatg cagaattctc ttggtgagct aaacggctac gtccaagaaa atatgacagg
     6541 ttttagtgta ttgaaagtat atggacgcga aaaagaaaca ttggaaggat tcaaaaaagt
     6601 taatcacagc ttgaaatact ttgggtttcg tgcagcattc atttctggct tgatgatgcc
     6661 tctagttcaa ttaactgcct atgctactta tattggtatg gctgttctag gaagctttta
     6721 tgttattact ggggcgatcg ttgtcggtca actacaagca ttcatccaat atatttggca
     6781 aatcagtcag ccaatgggga acgtgacaca attgtcttca gttttgcaaa gtgcatcagc
     6841 ggcaacgaaa cgtgtattcg agattttaga tgagccagaa gaaaaagaaa acaaagtaga
     6901 tgcttcactg ccagaaacga ttttaggaga tgtgacattc gatcatgtcg attttgcata
     6961 tgatccgaag aaaccattaa tccaagattt gaattttgaa gtgaaagctg gtcaaaccgt
     7021 agctgtggtc ggtccgaccg gtgccggcaa gacgacattg atcaatctat tgatgcgttt
     7081 ttatgacgta gacaaaggag caataaggat tgatggaatc gacaccaaat caatgagtcg
     7141 cagtgatgtg cgttcgctat ttggtatggt cttgcaagat gcatggcttt atcaaggaac
     7201 gattgcagat aatatccgct tcggaaagct tgatgcgaca gattatgaag tagttgatgc
     7261 tgcgaaaaca gcaaacgtag atcattttat ccgaacgatg ccagatggat atgatatggc
     7321 catcaattct gagggagaca atgtgtcttt aggacaaaaa cagttgctga cgattgcccg
     7381 agcagtggtc tccgatccta aaatcttgat tttagatgaa gcaacaagtt cagtagacac
     7441 ccgtttagaa gcgttgattc aaaaagcaat ggatcgcgtg atggagggac ggacaagttt
     7501 cgtcatcgct caccgtttat cgacaatccg tgatgcagat ctgattctag tgatggatca
     7561 aggtcagatc attgaaaaag gaacgcatga gagtttattg gcacaaggtg gattttatga
     7621 aaaactgtat aacagccaat ttgctgagga agcagaataa gtcatataag aataaaacag
     7681 tttaaaactt attttttgtt ctctattctt gataatggta aagtaaagtt atcaagaaaa
     7741 gtgaggacac ggagatggaa cagtttttaa atgaaaaaat tactgaagcc aaaagagaag
     7801 ttgatcgttt aacgcacgtc tgtcagacga agccttttga ttcagctgat ttttttgaaa
     7861 atgagttt
//
LOCUS       DARDHA010000129         7697 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000129 DARDHA010000000
VERSION     DARDHA010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.131"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..179)
                     /locus_tag="SDA10_002201"
     CDS             complement(<1..179)
                     /locus_tag="SDA10_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEG4241086.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
     gene            complement(192..1295)
                     /locus_tag="SDA10_002202"
     CDS             complement(192..1295)
                     /locus_tag="SDA10_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEG4241087.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1385..2341)
                     /locus_tag="SDA10_002203"
     CDS             complement(1385..2341)
                     /locus_tag="SDA10_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEG4241088.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2622..4556
                     /locus_tag="SDA10_002204"
     CDS             2622..4556
                     /locus_tag="SDA10_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG4241089.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5013..5429
                     /locus_tag="SDA10_002205"
     CDS             5013..5429
                     /locus_tag="SDA10_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEG4241090.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5443..6009
                     /locus_tag="SDA10_002206"
     CDS             5443..6009
                     /locus_tag="SDA10_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEG4241091.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6032..7018
                     /locus_tag="SDA10_002207"
     CDS             6032..7018
                     /locus_tag="SDA10_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEG4241092.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7173..>7697)
                     /gene="scm"
                     /locus_tag="SDA10_002208"
     CDS             complement(7173..>7697)
                     /gene="scm"
                     /locus_tag="SDA10_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEG4241093.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         2203 a         1473 c         1591 g         2430 t
ORIGIN      
        1 ttattgatag cggtagtcgc taaatcaaaa actagcattc caataaaaaa taaaaaagtg
       61 tttagccaat gtatctcatg aaaaaaagcg atagagaata atacccctac cgcaaaagga
      121 aatacactag ccacctttgt tcgtatttca actacttcta aaaagatttt tagtgtcatg
      181 gttcgtactc cttattttag ttcagaacgg tcacccatcg tgtaatggga gtcctccgat
      241 aatttgaagg tgtctttgat tggatcggtc acatatactt tatcgtcttt tgtcacgaag
      301 atcgcttccg tcccgttatt caattcgctt tcgacgtatt ccagtccttt tttcacaccc
      361 attgaaaaga ctgctgtaga caacccgtca ccatcaattg atttatctgt tatgatcgtg
      421 acacttgcga tatcattgtc gaacggataa cccgtttttg gatcaaacaa atgatggtat
      481 tttttgccat ctacttctaa ataacgctcg taaattccag aggtaactaa tgttttgttc
      541 cgttctttaa tagaacctaa aacagatccg cgagccatgt tcggatcttg gatgccgact
      601 gtccagtctt ggttttcgcc tcttgggctg tggcctaaga catagacatt accgcccaag
      661 tcaacgatgg cagtggtcac tccttgtttt ttcaagactt tcactacttc gtccgtaatg
      721 taacctttgg cgattgcacc taaatcgatg atcattcctt tttcttttag ataaacagtt
      781 ttttctttgt catttagttc gatcttatga taatccacta atttcaatgc ctgatcgatt
      841 tcttcttggg aaggttttct ggcgtcatca aaaccgattc gccataattg tgtgattgca
      901 ccaatcgcca tatcgaatcc gccttgagaa tcttggctat attcgtaggc ccgcttgact
      961 aacgtgtaga cgtcatcaga aacttttact ggtttgatcc cagcttgctc attcacttca
     1021 tcgatctcag aacctttttg attgatcgtg attttatcac caagttcttt tactcgatcg
     1081 aaggcaggtt tcaatgctga ttctttgcct tcatcataca cacggatacg aacgtaagtc
     1141 cctaacaaga actgttcatc agaatatgga tctttcaata tcttggtagc tgctgaactt
     1201 tcagtggttg ttttatttgc tgagttgctg cagccagtta gcaccataaa agagatgccc
     1261 agtgtgatta aggaaaatag tagttttttc cgcatctttt tcctctcttc attttgaaag
     1321 gtgagacgac tgcctaatct ggctatcgtc ccaccctcca acttttatta gtatgattat
     1381 gtgattattt ttctgttacg atattatcta cttcgattgt ttgagtgtcg cctttttcag
     1441 ctgcattcac taattgttgt gcgtacatga tgaatgaatg tgagctgtgt gtagcacctg
     1501 taacaacttc tacattagca ggtgagccat cttcttcgcc cattgctttg acgaattcgt
     1561 cgttcagagt agggatgtaa gtttttggtt ctgtaccgct ttttgctttc atattttcat
     1621 tatattctgt atcatcttgt tttgatttac cgtctttgtc gatataatca aagtttgatt
     1681 cagttacttt gccgccagca actgtcattt cgaattgcac acggtagccg ttagagtagt
     1741 tcttttcttc aagcttgtaa gtgccgtctt tcaaatcagc accattgtcg atttcgattg
     1801 tatctgtgtt accagcttga gcagcttgga tcaattgttg tgcgtagttt tggaagcttt
     1861 cagatgagtg agtagctcca gttacgactt ccacaccgct tgcgctttgt gcttttaaga
     1921 attggtcatt caactcagga atgtattttt ctgggtttgt gccagatttt gcttccatgt
     1981 ttttgttgta ttctgcatct tttgtttttg attcgccgtt ttcattgacg ttatcatatt
     2041 ttgattcagt gattttgccg tcttttacaa ccatttcaaa gactgcacgg taaccatttg
     2101 aataattttt ttcttctaat ttatatgtgc cgtcttgcaa atctccgcca gctacaactt
     2161 ttgcagatga ttctgttgtg ctttttgcag tcgaagattg tgctgtagat gattcagaag
     2221 aagagtttga gcttccgttg tcatctgcgc cgcaagcgcc taacactaga gctgaaaatg
     2281 ctaatacggc aaatccagtt acaaaacgtt ttttcttcat tttaataccc caccattcca
     2341 tttctagtcc acaatttttc tttgtgattc attatacatt ctaatctttt tagcctataa
     2401 gtcaactatt tttgataaat aacccatgta atatttcaaa cgttttcttt cagcaaattt
     2461 cgtgaaaaaa cgctttattt taaaatttga aaaaaatcca aaaggagtta gacaaaagaa
     2521 cagaacatct atataatata tggagattgt gtaataagta tcaaactaaa agaattatca
     2581 tatattcatt atatagatag tctataagga gcggatgatc aatggcaaag caaaatattg
     2641 tcgttgtagg agcaggatat gccggtgttt cagctacaaa atacttggca aagaaactga
     2701 aaaaagaaga tgtagcaatt actctgatcg atcgtcattc gtaccatact atgatgaccg
     2761 agttgcatga agtagctggc ggacgtgtag aaccagaagc aatccaatac gatctgcagc
     2821 atttgtttgc tcgtcaaaag aatgtacaac ttgtcacaga cactgtaatc ggtatcgata
     2881 aagaacgaaa aatcgtcaag accaaattag gttcctatcc ttttgatcag ctgatccttg
     2941 gaatgggcgg agagccgaat gactttggta cacctggtgt caaagcacac ggattcaccc
     3001 tttggtcatt tgaagattct gtaaggattc gggaacatat tgagaagaca gtagcaaaag
     3061 ctgcattgga accagatgat gaactgcgta aagccatgct gactttcgtt gtctgtggtt
     3121 ctggatttac cggtattgaa atgattgggg aactgatcga ctggaaaaat cgcctagcca
     3181 aagattataa attagatcca aacgaaatca cactgatggt cgtagaggca atgccgacga
     3241 tcttgaatat gttggatcga aatgatgctg caaaagctga acgctatttg cgtaagcaca
     3301 acgtggaatt attattgaat gcaccaatcg tagaagtggc tgctgatcat atcaaattga
     3361 aagacggacg caacattccg actcacacac tgatttggac agctggtgtc aaaggaactt
     3421 ctgacgctgc tgactttgga ttagaatcag cacgcggaca gcggttgatc gccaatgaat
     3481 atatgcaggc aaaaggctac gaagataaaa acatctatat cattggtgat ttagtctttt
     3541 atgaagaatt cccaggaaca ccgacaccgc agatcgttca agctgctgaa caaacaggac
     3601 acacagccgc agcgaatatc gtggcttcga tcaaaggcgg agaaaagcat aaatttaaag
     3661 gaaactatca aggatttatg gtttctattg gtgccaaatg gggggtagct aacctatttg
     3721 ataagatcca tttaagcgga tttttagcta tcgtaatgaa acatatcgtg aatttgaaat
     3781 acttctttga tattcgttct ggttattata tgttccaata tttaatgcat gagtttttcc
     3841 atatcaaaga tgatcgcaac gtgactcgag gacattcttc tcgttacggc aatgtcttat
     3901 ggagtgtgcc acttcgtatt ttctatggtt tagtttggtt cgttgaagct tccaaaaaaa
     3961 tcattgggaa tggtgaagtg ttcaaaccaa gtacttggtt tggtgaagga tcatggttca
     4021 ctggaaacag tgtgttccct tggacatggg aatacaatgt aacagatgtt gccagtggtg
     4081 cttctgctgc tggtgatgca accacagctg ccagcggagc aggggcaagt gccggagaag
     4141 cagcgactca agctgcacat tttggtttga gttatgcata cggtgaagaa ccaatggctg
     4201 tgattgagaa gtcaccagac tggttcacaa agatcatgca agtgatcatg ccaaacaatg
     4261 atgtagcaat gttcttccaa aaattcatgg tattctttga aattgccttg gcattagcat
     4321 tgattgctgg attattcaca tggctatgca gtgcgacaac gattgtattg gtgatcacgt
     4381 tctgcttatc aggcatgttt tactgggtaa acatttggtt catttttgtt gctttcgcat
     4441 tgatgaacgg ttccggacgt gcgattgggt tggatcgctg ggtaattcca tggctacaac
     4501 gtacactagg taattggtgg tacggtagac caagttccca gtacggcaag aaataaatag
     4561 ataaagaacg ttatttgaat gcaagatttc aatagaaagg ggctgagaca aaccgtcaca
     4621 gcctcctttt tatgtaaaat taaaggaata agatgcccga gataagcctc catgagcaag
     4681 acacaggtgt cacaacctct gttacaacct cttgttataa acggtgttcc agaagtagct
     4741 tcttcgaaaa ataagccgaa atttcacaaa aacttgaaaa gtaattttcg taaattcctt
     4801 cttatttctt gaagttgccc acttctgtca caacctcttg ttataaacgg tgttccacct
     4861 gctgcttctc ggtaataagc ccaaagaatt agaaaacatg gaaagctgtt ttctattctt
     4921 cgttcttatt actcaaagca agacacaggt gtcacaacct cttgttttta tgctaaaatt
     4981 gggtgagtta atctggaaaa aggagaacaa aaatgagcct taaagaattt gctgaaaaaa
     5041 gtctgctcaa accttgggat gtttttatta ttttgctttt gactgttctt tcttttttac
     5101 cagtcatgat ttttggtctt cagcaaaaca ccgcaacagc agaaaaagaa gcggtactgc
     5161 gtgtggatgg cacagaaatc aaaacattcc ctttgatggc tggagaagag agcaacacat
     5221 atacttacaa agatgaacac ggagattata acttgatcga gatagatggc gatcgagtac
     5281 ggataaaaga agcagattgc gatgaccaga tctgcgtacg tcgaggctgg gcaacgaaaa
     5341 atggcgaaac catcgtctgt ttgccacata aattagtgat cgaagtccgt tcaacagatg
     5401 gaggtgacga agacagtctg atttattaga ccttcacaca ccatgagtaa attacaaaaa
     5461 atgatttata tttcgctact tgttgcacaa ggagtgatca ttggtttatt ggaaaatatg
     5521 atcccttatc ctttttcttt tgcgcctggt gcaaaactag gcttagccaa tctaatcacg
     5581 atcgttgcca tttttaccat gaaaaaacaa gacagcttct tactactttg gctacgacta
     5641 ttcctgacta ctttgctagg cggaacgatt tccacattcc tttatagtat gagtggtgct
     5701 ttgcttagtt attttggaat gttattggtc aagcaattag gaccaaaacg tgtcagtatc
     5761 atcggaatta gtgcggccgg cggtttcatg cataatgtcg gacagctttt gatggcttct
     5821 ttcattgcac aatcatggac agtcatgttg tacttgccag tcctttcagt tttagggatt
     5881 ttatcaggga ttgcaatcgg gatcgcagcg aattatcttt tacgacatgt gcagacactt
     5941 caacgattcc aaaaagatta tgagcaggga accaaacaaa aatggagccc aatgtttacc
     6001 aaaaaataat cgtcacacaa ggagtttttt tatgaagcta catccaatgt gggaaaacta
     6061 tcctgcttta agtaaagaat tgacagaaac actaaaacgg atggaagaaa ctgtccgcct
     6121 gaaaaacaaa cctgtcgaac aagccatcaa agaaacgatc catgccggcg gaaagctgtt
     6181 gcgtcctgcc tatcagctat tgttctcaca atttggacca gagcaagatc gagacaaagc
     6241 catcgctttg gcagcttcta tcgaaatgct ccatacggcc acactgatcc atgatgatat
     6301 cgttgatgaa gcggatctgc gacgtggtca gcctaatatc cgtagtcagt ttggtaatgt
     6361 cgtttctgtc tatgcaggag actatttatt tgtctgctgt tttaaactat tgtctgatta
     6421 ctccacttcc ttgaaaagtc tccaattgaa ttctcgcagt atggaaaaag tattgaatgg
     6481 cgaattaggg cagatggatg atcgctataa ttatgaactt tctgtaaaag aatatttgga
     6541 aaatatttca ggtaagacag ctgaactgtt ccaattaagt tgttctgttg gcgcttttga
     6601 aagtggaaca agcgagcgct tcgcaaaaaa agcaggagat atcggacttt ctatcgggat
     6661 ggcctttcag attatcgatg atattttaga ttatacaaaa gagagccagg aaatcggcaa
     6721 acctgtacta gaagacatgc gtcaaggggt ctattcttta ccgttgatct actctttgca
     6781 aaaaaacaaa ccaaagctac taccttattt agagaagaaa gcggcaatga cagaagaaga
     6841 tgtcgacgcc gtacgcaaaa tcgttgaaca tacaggaggc gtggaagaag ctaggaagtt
     6901 agcggcctct tacacagaaa aggctttgaa ggaaataaag aaattacctg ccacctctct
     6961 tcgaacaaaa gagaatctct tttcccttac ccaactgatt ttggatcgca aagactgaat
     7021 agggaaaggt tcgatacgta aataaatttc tcagaaggaa atctacgtgt tttttgacaa
     7081 attgaattgt agttcttgta tttatgataa aaagcaaaaa taaagagcaa cgtccgttag
     7141 cgacgttgct cttttttatc ttgaatattt ttttaccttt tcgatttctt ataactcata
     7201 aaagtgattc ttaaaccgat tacaagtaat ataacgcctg cgagtgttat ccaattattc
     7261 gattgacttc cagttttagg aagtgctctt tttgatttgt ttccattgtc attgcttgtt
     7321 ttattagtta agttaaagtt cgtcctgtta tccgaacttg tttttgagaa ggagacactt
     7381 gcatgacgtg tatcgcttgt ggtagacgac gtgtctgtac tatcgcttga agttgatgaa
     7441 tctgagctat cacttgtggt agaggatgta tctgtactgt cacttgaagc tgacgtgtcc
     7501 gtgctatcac ttgtagtggt agatgaatct gagctatcac ttgtggtaga ggtagtatct
     7561 gtactgtcac ttgaagctga cgtgtccgtg ctatcacttg ttgtggtaga tgtatccgta
     7621 ctgtcgcttg aagctgacgt gtccgtgcta tcacttgtgg tagaggtagt atctgtactg
     7681 tcacttgaag ctgatgt
//
LOCUS       DARDHA010000130         7668 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000130 DARDHA010000000
VERSION     DARDHA010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.132"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..192
                     /locus_tag="SDA10_002209"
     CDS             <1..192
                     /locus_tag="SDA10_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241094.1"
                     /translation="MERLFLARTEKSEEPNYDLKPDEDEFYYVSRSIFQSMEMKYNFK
                     VNAYELSLLYELLGPYIQK"
     gene            269..757
                     /locus_tag="SDA10_002210"
     CDS             269..757
                     /locus_tag="SDA10_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4241095.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            769..1263
                     /locus_tag="SDA10_002211"
     CDS             769..1263
                     /locus_tag="SDA10_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4241096.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            1284..2093
                     /locus_tag="SDA10_002212"
     CDS             1284..2093
                     /locus_tag="SDA10_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4241097.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            2090..2911
                     /locus_tag="SDA10_002213"
     CDS             2090..2911
                     /locus_tag="SDA10_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG4241098.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            2962..5061
                     /locus_tag="SDA10_002214"
     CDS             2962..5061
                     /locus_tag="SDA10_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEG4241099.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            5058..6176
                     /locus_tag="SDA10_002215"
     CDS             5058..6176
                     /locus_tag="SDA10_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEG4241100.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            6215..7093
                     /locus_tag="SDA10_002216"
     CDS             6215..7093
                     /locus_tag="SDA10_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG4241101.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            7239..7436
                     /locus_tag="SDA10_002217"
     CDS             7239..7436
                     /locus_tag="SDA10_002217"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEG4241102.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         2313 a         1263 c         1739 g         2353 t
ORIGIN      
        1 atggaacgtt tattcttggc tcggacagaa aaatctgaag aaccgaatta tgatttgaaa
       61 ccagatgaag atgaatttta ttatgtttct agaagtattt ttcaatcaat ggagatgaaa
      121 tataatttta aagtcaatgc ttatgagctt tcgctgctct atgaattatt aggaccgtat
      181 attcaaaaat aaagtgtcta aaaaagtgtc taatctgttt tttttaggtt agacactttt
      241 tttaatgtga attaacgtga ttaaaccaat gtttttaaaa gttggcacag gaattgctta
      301 tataaaagtg aacagaaaga aaggaattaa ccatatgaag aaaattctaa tagcgactca
      361 cggtcactta gcttcaggct ttcttagcag tattcaatta ctgacgggga aggttcaaga
      421 gattacggtg attaatgctt atgtggatga gtgtgatttt gaacaggagt tgaatgtatt
      481 tgtaaaccag gtgaacgagc aggatcagat ttttgttttt accgatttat ttggcggaag
      541 tgtcaatcag aagattacca aaaccttttt ggagaaagag attgctgtaa ctattttgtc
      601 tggctttaat ctgccaattc tgctcgaaat agtattggca accaaggaat taacaaagga
      661 ggatattcag gaactggtaa ataaatgcca acaggaatta aagatgacca ttcttgatga
      721 ggtacatgta gtaaatgaag aggaagattt tttttaggag gaagaaaaat gattaaagca
      781 gtgcgaattg atgaacgatt aattcatgga caagtagcga tgacttggac aaagagtcta
      841 aatttaaccg ggttagtagt agcaagcgat gaggcggcta ccaacgatat tcaaaagatg
      901 acattgaaga tggctgcacc atcgaatgtc aaagtcatta ttaaaacagt tgatgacgcg
      961 attcatttgt taaaagatcc gcgggcgaag gacatgcgct tgatggtttt agtccctaat
     1021 gtcaaagatg cggtaaaagt tgcgaaagct ttcccaacgg aaatcgaatt aatgaacgtg
     1081 gggaatgctg ggaaaatgac accgccatcg gaagataaga aaaccttatc aaaggaagtg
     1141 atgttaacga cgtcagaaat tcaagactta aaagaattag ttgaacttta tccagatacc
     1201 gtttttcagc ctacaccggc gatggaaaaa cgttcagcta tctctatttt gagtgatttt
     1261 tagaaaaagg aaggagaaaa attatgtatg atgcatttgt agtcgctttg gccgttttta
     1321 ttggaattgc tggacatgaa atgttcggga tgtccatgct gagtcgtccg attgttgttg
     1381 ctccgttagc aggagcgttg atgggggatt tgcagacggg gttgattgtc ggggcgtcac
     1441 tagaagccat ctttatgggt gtcgtcaata tcggaatctc tagtacggcg gagccttctt
     1501 tggctgctgg gctggctaca gcgttcgcaa tcaaaatggg aggaaacgtg gatgcgatta
     1561 ttcccattgt ctttccacta gcagtattag gattacagtt aatgaatatg attttttctt
     1621 ttgtatgtgg accgatggct gctaaatttt tcaaatttgc aaaatctggt gatcaaaaag
     1681 gaatcatcaa tctgcacttc tttatgtggt ttgtccacta tgcgctttat gctcttattc
     1741 cattctttgc cgttttattt ggagcagatg ttgtcaaaag cgtcttggat aatattccag
     1801 aggtcatcat gaacggatta acagtcgctg gaaatctgct gccggcagtt gggatggcga
     1861 tgctgttacg gatgctttgg agtaaaaata ttgctgtgtg gtttttcttt ggggccgttg
     1921 ttatggcgta tctcgagtta ccattgattg caattgccgc agttggaacg attattgcaa
     1981 ttgttatagc ccaaagagat ctacaactaa aaaatatatc tgttcatgca gttcaaagcg
     2041 atgctgctgt aaatgcaacg cttgaccaag aagaggagga tttctttcaa tgaaattagc
     2101 tgatggaatt tctaaagatg agaaaaaatt ggttcggaag atgttttggc gctcgatgac
     2161 tatgtatgct tcggtgaatc cggtgacgat gggcggcggt ggtttcgcct attctatgat
     2221 gcccttcatt aatcatttct acaaaagtga agaagatcga gctgcggcgc tggaacgtca
     2281 tacacagtat ttttcgacga ccattccttg cgcgtccttt gtcatgggga ttgcggcatc
     2341 gatggagaag gaaaattctg aaaaagctgg ttttgacgtg gaaagtatca acgcgatcaa
     2401 aacgagtttg atgggaccat tggctggaat tggcgattca ttgttctggg gagtttggcg
     2461 tgtcgttacc gcgggactag cgatcaactt agccaatgcc ggaaatattt tggcaccaat
     2521 tatcttttta gttatgttca atatacctaa ttacttgatg cgatactatg gcggcgtttt
     2581 aggctattca ttaggctcta aatatattga aaagctgtat gcgaacgggt taattgaaat
     2641 tctgacaaaa gctgctagta tcttgggtct gatcatggta ggtgcaatga cgatcagtat
     2701 ggtcaaattc aataccgttt tgagcttttc aatgaagggc gaagaactaa tgaatgtcca
     2761 agaaatcctc gatagtattt ttaaaggaat tgtaccgtta ttggtaacat tgagctgttt
     2821 caaattacta gataaaaaag tcggcgtcat caaaattatt tttggaatgg tcgtcttggg
     2881 aattttattg tcattacttg gaatcgcata gtaggaagct gcagctacta ggctgcagtt
     2941 tttttagcaa ggagggaaaa gatgactatt aatgaagtaa tttattcacc caaacaccca
     3001 ttttttaatg ctgagttaaa cgacggaaat gtgactactt caaacgaata cagctactat
     3061 cggaatattc ctgatgtaaa gggattgacc ttcgatgtaa gtaatcaaaa gacattagca
     3121 aaccttgatg tttgcgggac gattcaaacg ttgacctttt atcgagaaaa tttactaact
     3181 gaggaaaaac caggtgtttg ggtcaataaa cagctctctc aaaccaattc gttatcctta
     3241 aaggttgaag taaacgggat tgtctaccat ttgtcagaat cggatcatcg tattgaagtc
     3301 gatgtgattc aagactgttt gcctagatat attcatcatt attcgacgtt taaggtcatt
     3361 gtagtatcct ttgctcccat actggctaat aagcgtttaa gtatgcttgt tcaacaggtt
     3421 tacattgtga atgaaacaca cgaaagcctt caagtttcag tgaaagatgt gccttcgtat
     3481 caagagaagt attcggatca gcaaaatgtg ttgatttctc aaaaaggaaa taaggacacg
     3541 atcgcacaac aggaagtggc aagcttcgcc attgcttttg tggacccaaa tgcttttgaa
     3601 gaagtaatga cttttcagga atctgatatc gaaaaatggc tgaaggaaac attgaactat
     3661 tttgctcagc tatttgggca gctgtcaatg ccaaacgata gagttgtcca tctctatcgt
     3721 cgtgcacttt atcaaagctt ttctagtttc ggtatgaatc gacagggaca ggtcgtcggc
     3781 agcaattggg gcagctatcc tgctactaat cgaatttgga ataaggatat gtattacgca
     3841 tcattgccat tcaccatgtt tgaaccggag ttatgtcaaa aaacggttct atggtttgat
     3901 caatatggcg ttaagtttcc tggttcaaaa tttacaggag gtatcaatca ttctctgagt
     3961 aattcgttat ccagtgcgtt actggctagt ctttattttg aacatactgg ggatacaaaa
     4021 ttctttgagc ggtgtccgca ggttctactg aatgcttcaa gaattattga ggacatatta
     4081 gctcaacgtc ggtcagggga accaatgctt tttccatctg tttggttatc agacgccttt
     4141 gcacttggaa agtatcatac cggcagcaat ctttgtcttt ggaaggcctg tattggactt
     4201 gcagaaattt ttgaagccct agaacaactg agtcttagca agcgatacaa aaacattgcc
     4261 tgtaaactaa aggaatcaat ttgtaaacaa atgactatcg atggtccttt tggtgagcaa
     4321 tttttggagg gaatcggtga tgaggagaaa acaacctatt cagtgaagca ctatcaaaaa
     4381 ccgattcttg agcaagggtt aatcttttta agtgatgtga ttgtggatgg aaaaattaat
     4441 ttattgatgc atgatggaga agaatcagac actacgttga tgccttttta tcaattttta
     4501 gataataaag atcatttata tcagaacacc atggcctttt ctgctagttc tttcaatcca
     4561 acctatagcg aggaaatcaa aggcatcact tggggattag aatccggcgc cacttttcct
     4621 ggctttatca ctgttttaat gagcgagctg cacaaccctc aagctttcgt aacccgatta
     4681 gaagaactaa tttgtttggc agatttagac ggttcgtggt ggtggtggcc gtatcgctta
     4741 gaggctcaac aaggagaagt cgtgcgagac tttggctgcg gcaaatgcgg ctgggcatcg
     4801 ggtctttttg tcagcctaat gatcagtcaa tatttcggat tgaaattcaa aaaaggagta
     4861 ttgcaaattg atcctgttga taatttaacg ttttcttgga agaatttgaa gttcggtcaa
     4921 gccttcattt cgattgaatg tacgcaatcc gagttgagta tttcaaattt gtcagaaaag
     4981 ccattgaaaa taagtgatac gaaaaaaatt cttcacgtag aaaagggaaa aacgattcac
     5041 attcaaagga gaaaaagatg agtatactaa aaaatattac ccaatttaaa aaaatccaga
     5101 gtcgagcaat cactcccgaa aatccaacag gcgagaaagg taaagctgcg atgttgccaa
     5161 gcgagctagg accaagccgc aaaggtcgtg gcgcaattcc gctgacttct ggtgaagaaa
     5221 ctgtgattgc ggatattact ggaacgggaa taattcgtca tatgtggatg acgattcgtg
     5281 atcgaacaga atttggcagt tttgttttac gtgatttaat cttaagaatt tattgggatg
     5341 ggtcggatag ccctgctgtt gaaagtcctt taggggattt cttttgtaat gggtttggag
     5401 agcgttgcga ggtgaactcg ttgccgatag tggttaatcc tacagggggc tttaattcct
     5461 atttcgaaat gcctttccaa acatccgcta aaattacgat cgagaattta catccgaagg
     5521 atatgtcctc atttttttat acgataaatt acgctttggt ggacgaattg gaagaaaata
     5581 gcctatattt tcacgcaaat tggcatcggg aacgcattac caaattacag gaggattata
     5641 agttaattga tcgattaaag ggtcaaggct attacgtggg tacttacctt gcgcttacct
     5701 gcctagaacg ttattggtgg ggcgaaggcg agtttaaatt ctacattgat ggggatagtg
     5761 agtatccgac cgtaacctca acaggttcag aagattattt tggcggggcg tgggcctttc
     5821 atgagtacga tgagttcgga cgtccacatg cgaaaaacta ttcaacagcc tttttagggt
     5881 atccttacca atcaaataga gatgccacaa gagatcgttt tgaaacggga aaattgaatg
     5941 ccgttcatgc ctttggtgac gacgcaatgc cacgacatgg tctttatcgt tggcatcttc
     6001 aagaccccat ctgttttacc gaagatttat cggtcacttt acaacaaatc ggcaacgatg
     6061 atgttcgctt atttgagcgg caagacgatg ttgctagtgt cgcttactgg tattcaacag
     6121 aagcttcagg aaatactaaa cgtcatttca ctgatcgaga acgcttgccg cgttaatgaa
     6181 gtgtttttca catattcgaa gggaggaacc tatgatgata tatggggcaa ttgaagctgg
     6241 agggactaaa tttgtctgtg cagtcggtac tgaggaatta acgattttgg aaagagtcag
     6301 ttttcccaca acaactccag ttgaaacaat gaagcaggtc attgattttt ttaaaccttt
     6361 taaggaacaa ttggcaggca tcggggtcgg ctgctttggt ccaattgata ttcaatgcaa
     6421 atcaacgagt ttcggtcata tcacatcaac accaaaactt gcttggcaaa atttcaattt
     6481 tgtaggaacg ctaaagcaat atttcaatat tccgattgaa tggacaacag atgtcaatgc
     6541 ggcttgctat ggtgaatatg ttgctggttc tggcaaaggg ttagcaggtg ttgcatactt
     6601 cactgtggga acaggtatcg gaggtggtgc cttgttcaat gggactttcg tggaagggtt
     6661 cagtcatccg gaaatggggc atatgttgat caagcgacat ccaaaggaca atttttcagg
     6721 aaactgtccc ttccaccaag attgtttaga ggggatggca gcaggaccag cgattgaaaa
     6781 acggttgggt gtaaaaggcc agaatttatt ggctgacgat tcgttttggc agattgaagc
     6841 cttttacctt gcccaatgtg catacaatac aacactgatg ttttcgccag atagaattat
     6901 ttttggcggt ggtgtaatga aacaagaaca catgaagaaa aaagttcagg ataaatttgt
     6961 tgagcttata aatggatatg ttgaaatacc accaattgat agctacatta ttacgcccga
     7021 attaggtgat aatgccggaa taatcggcgg attagcattg gccagaaaag cggtaagaaa
     7081 taagcaacca taattaatga atgtttcatt gatactcatg tcaaaagtat ttaaccgagt
     7141 aatcaaatga tcaaatgaag ttggataaag cgaaaatgaa ttcaaataaa aaggctctaa
     7201 gtcaaatcgt gttgttttca ttaaatcact gaaaggaaat gcttctgcgt ttcctttttt
     7261 ctttttgttg tgcgctgtat accttgagga tattcactgg tggcgtacac ttaaatagta
     7321 atatattgtg ttactttatg tctgtggtaa tagttatagt tgcttaccaa tgtatgttca
     7381 tttttcgaaa tgttgacttc ataatccttg gcttaatgtt tgaatttttt tggtgataga
     7441 ttttttcagc cgtataaaat aaatcgtaat tctattatag agagttgtga ttataaaaag
     7501 atagcaagaa aaaaaaagaa agtttctata cattttaaat attttattcc tatttctatt
     7561 gctaaaaaag tagaaattct atatttacct ttaactctcg cttctcttgc aaatgtagtt
     7621 agcattttaa tcgataataa actcaggtag ttatattacc ttgaaatg
//
LOCUS       DARDHA010000131         7643 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000131 DARDHA010000000
VERSION     DARDHA010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.133"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(97..1245)
                     /gene="nagA"
                     /locus_tag="SDA10_002218"
     CDS             complement(97..1245)
                     /gene="nagA"
                     /locus_tag="SDA10_002218"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEG4241103.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1321..2157)
                     /locus_tag="SDA10_002219"
     CDS             complement(1321..2157)
                     /locus_tag="SDA10_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4241104.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2341..3558)
                     /locus_tag="SDA10_002220"
     CDS             complement(2341..3558)
                     /locus_tag="SDA10_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG4241105.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3707..3886)
                     /locus_tag="SDA10_002221"
     CDS             complement(3707..3886)
                     /locus_tag="SDA10_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241106.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3941..4024
                     /locus_tag="SDA10_002222"
     tRNA            3941..4024
                     /locus_tag="SDA10_002222"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3975..3977,aa:Leu,seq:gag)
     gene            complement(4264..5634)
                     /locus_tag="SDA10_002223"
     CDS             complement(4264..5634)
                     /locus_tag="SDA10_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241107.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(6348..6638)
                     /locus_tag="SDA10_002224"
     CDS             complement(6348..6638)
                     /locus_tag="SDA10_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241108.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(6846..7538)
                     /locus_tag="SDA10_002225"
     CDS             complement(6846..7538)
                     /locus_tag="SDA10_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG4241109.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         2324 a         1518 c         1179 g         2622 t
ORIGIN      
        1 acttttgaaa cttatgtaaa gtgattttta ctagaacaca aaaaaacctt aaaaccaaac
       61 ttaagaagtt tagttttaag gttaacttaa attatattat gcgtggaaac gttccacgcc
      121 atctagataa gttgcttcca atgtcatatc agggttcaat acgataaagt ctgcatcgcg
      181 tccttgagcg atttttccac atgtatcatc gattttacag ctgattgctg gaatcagact
      241 agccatcatg actgcttgtt ctggtgttgc taatccccag tctacaacat ttttaattgc
      301 ttctttcaat ttcaatatac ttccagctaa gttgccagat tctagacgtg ctgttccttc
      361 ttttactact actggaaatt cgcctaaatt gtaatttcca tcaggcatcc caccggccat
      421 catacagtct gtaattaatg cgacatgatc atgtcctgct ttttccatta ggattcccgc
      481 agcatttgga tgtacatgat ggccatcaca aatcaattca gaaaagacat gttctaaaga
      541 taataacgca cccaccatac ctggttcgcg atgattcaat ccacgcatac cattgaaagc
      601 atggacaaat acacttgcgc ctgcttcaac agctttttgc gcttcttcta gtgtagcatt
      661 actgtgtcct agagaaacaa ctactccttc ttcagtcact tgtttgacaa actcttctac
      721 accttgacgt tccggtgcta aagcaatttt tttgattaaa ccacctgaag cttcttgcca
      781 ttcatggaaa gtatccaaat caggatcccc gaaataactt gggttttgtg cccctttgtg
      841 ttcttctgtg aagaaaggac cttcaaaata gattcctttg attttagctc ctggtgcttc
      901 ctgatacatt ttgccgattg tctcagctac atcttttaaa cgttctttgc ttgacgtcaa
      961 tgttgttggt aaaaatgaag tcactccaca agataacaga ccttcagaca tcactttgat
     1021 gccttctgca tcattatcca ttacatcatg attcatatag ccgtgaatat gtgtatctac
     1081 caaaccagga gcaatccatt tgccgctttg gtcaatgatg ttgacttctc cctcaggtag
     1141 ttctttggtg tattcaccaa aaagtccatc ggtaatttca agatagcctg catgtttgac
     1201 acctgacgct aaaaagaatt tgtcagcata gataaaagtt ctcattctat tctctccctt
     1261 tgtttttcta cctctaaagt actcttattt agagaagaag tcaagaatgg tctacaccat
     1321 ttacaaattt tttgaaattt gtttcacagc ttgtgctaag ccttcataga atgctgctct
     1381 ttctggttct tctttacgct tgtatattaa gcttttaagc acaaataaat cattgaggta
     1441 atagtagcta ttcttctctt gagcccaata gattccttga ttcacctgtt ctaacgcttc
     1501 gtcgatttca ttttgatgga ataagaaatt tccataatta taaaaaattt gcgaaaattc
     1561 actttttgat cgttcatcag ctgattgatg cagcagttgg atgcttcgac tcaaatagta
     1621 gcgtgcttca gccgtttttc ctgccacacc atatattttt ccgatacaac taatcagttg
     1681 tatttctgtg tcagaaacat ggattctttc tttttggcta gagtagctta atgcttcttt
     1741 caaataataa agggactgag agatattttg acgcaacgtg aattcgcaaa caccgagata
     1801 ataataatag cgttgaaagt cagttgcttg atacagattt tcagaaatat cagaagattt
     1861 tagtacttgt tctaacttcc gataatcacg tttttggaaa caactatcca acagttcaaa
     1921 taatttatcg tttgtccgag tagtttttcg agagagattc atcacatgat caatcgttac
     1981 ccctagccgt tgacaaatct gcatcatcac caatacattc ggcagttcct cattattttc
     2041 gatccgactt aatacacttt gtgaacaaat atcatgcgaa agcatttttt gcgtcatttt
     2101 ttgttctttt cgaatctctt taatgatcgt tccaaaggac tcgattaacg tttccataag
     2161 tatcctcccc aaaatatgcg tattcgcatt ctattcgtat tttactttag gaagtcgcaa
     2221 acttcaagaa aagacttgca actaaataat atactttagt tatttaagaa aactccacta
     2281 gaaagagagc ctcagtgtgt ttgtctgtgg ccctcctact gaaaaatttg gtttttaaaa
     2341 tcatttttgg cgataggttg ctaggaattc tctcattttc ttagctgttt gtttcaaatc
     2401 atccatttta ggaagataaa cgattcgaaa atgatctggc tggttccaat taaatccacc
     2461 accatgtaca agtagaatat gatgttcatg taaaaagtcc aaaacgaatt tttcatcatc
     2521 caaaatattg aatcgctttg tatcaatttt tgggaatata taaaaagcag cttttggttt
     2581 tacggctgaa agaccaggaa tatcattgat tgcattatag atatattctc tttgttcata
     2641 gatccttccg cctggcagga gcagctcatc gacactttga taaccgccta gcgcggtttg
     2701 gataatttgc tgagacaata cattggaaca caaacgcatg gaggacaaca tattcagccc
     2761 ttctatatag cctttgacgt gtgacttatc tccacttagg accatccagc ctactcgaaa
     2821 tcctgctaca cgatgagatt tggatagacc gttcagcgta acaacgaaca aatcaggcgc
     2881 taatgtagca atcggaatat gttctagacc atccatcact aagcgatcat agatttcgtc
     2941 ggaataaata atcaggttat tttgtcgagc tacttcaacg atttcttcta ataattcttt
     3001 tggataaagt gccccagtag gattattagg attaatgata acaatagctt ttgtccgact
     3061 cgttattttt gatttgatat catcaatatc aggataccat tctgcctctt catcgcaaat
     3121 ataatgaaca ggtgtgcctc cagctaatgc aatagaagcc gtccacaacg gatagtccgg
     3181 cattggaacc agtacttcat ctccattgtc cagcaacccc tgcatacaca tcgtgatcaa
     3241 ttcgctcact ccgttgccgg tgtagatatc attgatcgtc acattcggaa aatgtttcag
     3301 ttggcaatat tgctcaattg cttttctagc agaaaagatc cccttggaat ctgagtagcc
     3361 ttctgagtca cgggcattca tgatcaaatc acgaatgact tcgtttggtg cttcaaatcc
     3421 aaaaggtgcg ggatttcccg tgttcaattt taaaatacta atgccctctt cttgcatccg
     3481 ctccgcttct tctagaacgg gaccgcgaac atcgtaactg acaccttcta atttgttgga
     3541 tttttcaaat tctctcatca cttagccaat ccctcatttc aaaaagttag aactttccaa
     3601 attttttgtt atttactatt tcaatatcat actccatcct agaaaatttt gccacttatt
     3661 taggaaaaac gtaagtatta aaaaaacaca aacaagcgtt gagaacttaa ttgtcatgat
     3721 agtcattcca tagattcgct gcccaatcca ttgcaattcc acaaatgatt cctagcataa
     3781 aaccaagcaa aaaacgtccg aatgcaagtc caatcaatac acctaagatc attcccagtc
     3841 ctgaaaattc tccgtcatat ttctccatct taataagtcc tcccattttt gagcttgaca
     3901 actatgtaaa acctcgctat cattagtgtg ttattattac gcagatgtgt tggaattggc
     3961 agacaagcat gattgagggt catgtgagcg ttagctcgtg tgggttcgag tcccatcatc
     4021 tgcactacaa ctcgtgaagt taattttgct tcacgagttt ttttgtttga acgtttcttt
     4081 atgccattat atacatatca ctgtatcctt tacaaaaaga acgtacaatc atgtaaacag
     4141 cctgaggtga gtatatgaag gtcggaaaca agctactcgt agaaacttgc aagtgttaaa
     4201 acaattaatc gaaaaacgag taaaatccct cacctaatac taaagcgagg gattttttgt
     4261 atattatctg tttatcaccg ttattataaa ggtacaaata ccaagcacgg ttaatccaaa
     4321 ttgcataaat tcattttttt gtacactcat gcttttcatt agctctttaa ctgccattag
     4381 tactaagatg gcgataacaa cataaagtat atagtacgtc cgcattccgg aagcccatac
     4441 tgtgggagaa aatcccataa ttaaccttga acaaaatccc attatcatca aaataactaa
     4501 aaaaatagaa gttttcttat tgtcaaaact taaataaatt ccaacaatta aacatagtaa
     4561 taatgccaag actactaaag tagctatcca agtacctgga taaaaaagtg acagtttcgt
     4621 tcctaactct gtaaagagat tattcaggtt actactactc caaatcattg cccttttatt
     4681 tccattgacg taagtaaagc tttgacccat tgtattacca aaataaataa tcaggtttaa
     4741 gaaaaacggt aatgcagtca atatacgcac ataatagttt tgacatttcc tatatgttag
     4801 aaggaaaatt aagaaaaata gcaataaaaa taagatattc atgtctaaaa aaaacggctt
     4861 tccaaatgat gaaataccta aatctaactt attaagaaag ttgaaatttt tatattcagg
     4921 aaaccattta ttaatttcat gtgcagcacg taatgcattc cctggagttg tcaaagagaa
     4981 aattaattct gctaagctta ttattgaaaa tactgataac ttataattat atcgtccatt
     5041 gacaatcaaa tataaactaa ctatacttgt tagaacaaaa aaacaggcgt tgacttgttc
     5101 ttgattagtt aaaaatatca ataaaggaat acttacccaa tatatttttc tatttatttc
     5161 ttctcctctt aagagttgaa aaaacggata aaaagttagt aaactaaatg ctactggcca
     5221 ctgatagttc aaggtggttg ctctccaacc ggtttccccc attactgtca atggtattaa
     5281 acagtatata gagcaaacca ataataaatt tttaacattt attttctcaa aaacatactt
     5341 gcaaagtaat actacaattc ctaacataat cgtagaattt aataatttcc atagcataaa
     5401 gtgtttagaa aagaacatta agaatagttc tataataatt cttgaactcc atgtcttata
     5461 atgttctatc gaagcaccca aatagccaag ttcatctaat ctacttgcat aaactacgtc
     5521 atccgctctc atagtcggca tatacatatt aaagagtaaa aagatacccc aaatccctat
     5581 tagtaacaag atcagttctt tgttattcgt tatttttcta ttatgtttgt tcatttttat
     5641 actccttctt atatttccta atagagtata acatagcttt aagcattact taaagataac
     5701 ttaaaagtta tcttttaaat acttaattgt taaatacctt atttgatgca aaagaaatct
     5761 acgatagtca acaaaaaaat tttcattata tccagaaaaa tttttcttag ttaaggacct
     5821 atggattgca gtaatgcaga attcgtcgaa taaactgcca aagacataca atcatattgc
     5881 ttttaaaatt gacgaacaag atatagattc gtttgtctca aaaatccaaa tgttaggttt
     5941 aactgtcgaa ccaggacgtt ctcgtgtgaa aggcgaggct tcttcaattt acttttacga
     6001 ctatgataat cacctcttcg agttacatac cggtactctc caggaacgtt taaaatccta
     6061 caattccact acctgagact agtaatgact agtctctttt tatggcctct gatcccccca
     6121 ttgagcgaat actaataggg cttcgaattg atctgttcaa tctttaagca atatccctca
     6181 aatgagtttg gaaagtttag tgattcatca caaatactta aatacatgta gttatatata
     6241 gattgaggtt cttgtgagcg tatgctcgtg tgggttcaaa tcccatcgtc tgcatccatc
     6301 tttccaaaac aagcggctag gacatttttg tctcagccgc ttgttttcta gtttattctt
     6361 tcatcttgtc ggaccttttt tcttgggtat agacttttct catattctac cacaattttg
     6421 ggtttcccta acacatgata ggatctcaca acaaattgct ttctattatt ataataacct
     6481 gtaacgacta acttcatccc ttcgtctact tcagctagga agttcaaact atgggaagca
     6541 atcaaacaat tgaccccatt taaactgaaa cggatcaacg gtctggcact aacttgcaaa
     6601 gtacggatct tatctactgt tccatatttt ttctccataa ccttcacctc aataccatta
     6661 tacgaacata cattcccttt gtaaagaaga aactctgttt tatctttttg actttttgtc
     6721 tattaccttt cttaaaatca tattggcact tcctgcgaag atgaaggaca tgtactaaaa
     6781 aaaagaggca gcagtataga ttactgttgc ttctttacta atttaggttt acttgttaag
     6841 aaagattatt tacgcaaatg ttcttcaaca atttgctgag atttatcagc tgtatcttcc
     6901 acttcatctt ttgctttttc taacttttga atatattgtt tcaagcttga gttttcagca
     6961 gttgcttcgt ctaatttctt ttgtgtatct gttaactgag tttctacatt tgttttttct
     7021 tgtgtcagtg ttgcgatttg ttgattcaaa ccatttacag tattttgtaa ttctgaaagt
     7081 tttgcttgtg cttcttgtag tcttgctgtc caatctgcat tcagatgatt gatttcatca
     7141 tttttttggt tgatctcatc tagtttgaca ttgatttcat tttgtttgct ttgtagttgt
     7201 gcatccttgc tgactaattg actttctaag ttttgttttt ctgctgccga ttgatttttt
     7261 tggttttcta attcatttac ttttgtttgg tattcttgga catctgcctt tgaatcttgc
     7321 aatgtctgtt ctttttgtgc tagtcgagcg gtaagatctg taagcgcttg ctctttatct
     7381 gtcaatgtat gatcaagctg tgtcagattc tcattgattg tatcaatatc tgtctctcca
     7441 ccccacaaaa taatttcatt tgcgtaccgc gtgcctgtgt agccaattag acatagaaat
     7501 gcaacgacaa tcccaccagt aataaatttc tttttcattt gcatagctcc ttaatccaat
     7561 acttttacat gctaaaagta agtgatctca aacacaatgt caagcgtttt taaattacat
     7621 gtgtaatttt aatataaata cta
//
LOCUS       DARDHA010000132         7612 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000132 DARDHA010000000
VERSION     DARDHA010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.134"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(62..790)
                     /locus_tag="SDA10_002226"
     CDS             complement(62..790)
                     /locus_tag="SDA10_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4241110.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(803..2083)
                     /locus_tag="SDA10_002227"
     CDS             complement(803..2083)
                     /locus_tag="SDA10_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG4241111.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2224..2826)
                     /locus_tag="SDA10_002228"
     CDS             complement(2224..2826)
                     /locus_tag="SDA10_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEG4241112.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3071..3277
                     /locus_tag="SDA10_002229"
     CDS             3071..3277
                     /locus_tag="SDA10_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241113.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3589..4308
                     /locus_tag="SDA10_002230"
     CDS             3589..4308
                     /locus_tag="SDA10_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG4241114.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4389..5072)
                     /locus_tag="SDA10_002231"
     CDS             complement(4389..5072)
                     /locus_tag="SDA10_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEG4241115.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5290..7476
                     /locus_tag="SDA10_002232"
     CDS             5290..7476
                     /locus_tag="SDA10_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEG4241116.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2729 a         1283 c         1431 g         2169 t
ORIGIN      
        1 aaaagaaccg aagggtttgg atcggctggt catttagtcg gtccaaacta ttcggttctt
       61 tttatgaaat caatttcttt aatccacgga tagaggctgg gatggtctcg ccgtttgcca
      121 acgtgacttc tctggttttt ttattaatcg accgcacatt ttgtgtattg ataacatagg
      181 atttatgaca acggataaaa ttccgatgca ttttctcgat ttctttgatc ttctcataga
      241 attgtaattg ggcatgagtc aaatggactt ccagtttatg tggcgctcct agcgaagtag
      301 aaaaatattg gatgtctttg atcggtaaaa atttgatctc atcattaaac tctattttga
      361 agatatcttt ttggatttgg gacatttgaa aatcttcgaa aattttgctc aggatatgct
      421 cgacattttc ttttaaatca taaagatctt ctttgacgat ataatcagtc ggttcgatgt
      481 tgctttttaa gattttcatc gacatttccg tatgggaagt cacatagatt atttttccta
      541 aaggatctaa ttcacggatc aatttaccga tcgctacacc atccatttca ctgtcttcta
      601 gttcgatatc taaaaagtaa ataccaaaag aagaagttct gatcttggct gcattgagaa
      661 gttcttccgg atcagaggta gaaatatgga cataactgtc taagttttca atcatgattc
      721 tatttttgat caccgttgat aaaacttcta attgttttgg atcgtcttca caaatataaa
      781 tgggaatcat atcattactc ctttatttta tttgtatata aatcgtttga gaaaaacgct
      841 ggttttcaat tgatgtttca aggaaaagat aaggattggt ctttgtcaat tcatccaaga
      901 taaacaagcc aaggccttcg ttttttggat ttgtttttgt tgagaaccct ttttgtttca
      961 actggtttaa cggtaatgta tttgttgttg tattgatcac acgaatcatc acggaatgat
     1021 tcttcttgag tatggtcaat tcgatttttt tcttctttgc agcactagct ccttcaatcg
     1081 cattgtccaa gataatacca agcatacgta caaggtttac cgtatgtttc gtatccagtt
     1141 cgattggctc aggaaaatga agctggacga tcaacccttt ttcctgtgcc atcatgagtt
     1201 ttaatgacag taaactacgg atctcaggaa ctttcatatt atccaacata cttagattgg
     1261 ccggaaaaag gttcatcatc tggcgtgtag gtaatatcgt ttgatcataa taagaccgta
     1321 atccttctaa gtcgccttct gaaatatagc tgtgaagaga aaaaagcaga ttttggtaat
     1381 catgtttgaa ttctctcaag tcttgatagt ttttttctag atctttgata taatcctgtt
     1441 gcatcctgac ttgttgctct aataactgat cagctaattt cttattagag attttccaat
     1501 aataaaccaa gatgatcagg ccaacacaca gatatagaat gaaaaaactc gtattcaaag
     1561 ctaagacatc aggtgtttca ccagaaaaac gggtataaaa aataatgata tagtatgtaa
     1621 ataatatcaa tgtcccgatc gtaccgatca tacggcggtc cacagtgtga aaccaagtct
     1681 ctaacgattt ttttaaacaa aaagctaata aagcagataa aaaagcggat aagacaatgt
     1741 gaaaatactg caatgaatct cctggttcaa taaccgaaga tttcaaaaca aaaaaatcgt
     1801 cgatgcttgt taagtgatct cctaaaagcg acgtcaataa cccaaaagga agcgcgtaaa
     1861 ccaattgttg acgcgaagtc gaaaagaaga aaaaagtaag catcagcatt cctatagcaa
     1921 ttagagcagt aaaaatccct aaaatcgaat agacccatcc aatcaaaata caagaaatca
     1981 gaatgactcc cgcaaacaga tatttgtatc ggatgtgatg gtcaatcaat aagatacaaa
     2041 ttgtgtaaat caaatattgc aacgctatgg accataaaaa cataaagtac agctcccatt
     2101 gatttttacc aaattatagc acaattagcg tattattttt ttaatttttc agggattttg
     2161 aattcagaac tcataccaaa gcagcagcca gatacaaaaa agtcagataa gttagttaat
     2221 tgtttaatga ttgttttttt catgaaagat tcctccaaaa ttcatcaagt agtttgtaat
     2281 aggaagcaac gtagctattt ctaaaacaat cccaagtagc aaataggtag aaaagtaaga
     2341 agctgagaaa aataaagtaa tgagagaaaa aattagaaac atgctgatcg ccttctttaa
     2401 taatggggga cgtttctcgg gttcaatggg attgattgct gtacctcttg atccatagcg
     2461 aagcagtaac aaaaagcagc tgattaacaa aaagaccatc attgggaagg agatagtcaa
     2521 ttgcagatgt ttcatcacca agggaacgcc ccataaataa agtagtgtcc acagcagaca
     2581 aacgaattca gagtcggaat gcaatccata agcatatctg cggatgcaga tataagaaag
     2641 aaaggtgatt cctgtttccg cccatgagcc tgtcaccaga gaaaagacta gaacgaccgc
     2701 tagttttcca agattattca ataaaacttc taaggcaaat tttacttgga catacaccat
     2761 atcttcttcc gatttgtctc cgctgaataa ggcttctaat aatttgttca caactttatc
     2821 tttcatctca tacgtacctc caccacaaat tttatgctaa aaaaaataaa aaacgtcttt
     2881 tgtttcataa aaagtggttt tgacatcaaa accgtcatga tctcctaaaa aaatcttcgt
     2941 tttccaagaa ccttaccaaa aaaagtgaca gttttgatac gaaaagtact ttttatgaca
     3001 agtttgtgct ttttcgagtg atttatgtta aaaaattact gaccttataa aaggtgatgg
     3061 agggattctt atggaaaatg aacaattgtt tgaagtcggc gaaacatatg catttaaatc
     3121 tgatttattt gaatcaccaa taaaaggaaa aatagaaaaa atctatgaaa atagtgtgat
     3181 gatacgtgtc atgaaatgcg cagtagaaga caatgatacc gctcataatc tgcactggca
     3241 gttagttgta cgcaaatcaa gtacaataga agaataaaaa cagataaaat gttgagagca
     3301 taaagaggtt gtgagtctcc cgtttagctc tgagttacaa ggaataatgt taaaaaacag
     3361 cttttcatgt tttatgaata ttgccttgtg accgaggtgt tggcaggaga atactgttta
     3421 tttgaaaaaa gggctgcgac aagacttttg tcacagcctt tattttcatg aataggagtt
     3481 acttttggct acttttagag aagtagcttt taaggagcag ataaagtcaa ggcttttatt
     3541 tctataaatg aagtagtaaa atgaagaaca gattttatta caggaaggat gccgataatt
     3601 gtgatcattg ttgcaggaac gatcggtgca gggaaaagca cactaactga aatgcttgcc
     3661 caagatttag aaacaaaacc tttttatgag aacgtggaag ataatgaagt attgccgcta
     3721 ttttattcta atccagaaaa gtatactttt ttattacaga ttttcttttt gaacaaacgc
     3781 tttttggcaa tcaaagatgc tttcagccat gatgacaacg tactagaccg ctccatctac
     3841 gaagatagta tgctgtttca tttgaatgca gatttagggc gtgtttctga agtagaagta
     3901 aagcagtatg aaggtctttt agaaacgatg ctgaaggagt tagaagaaat ttcgccacaa
     3961 aagaaaccag atctactggt ttatattcgt gtatcctttg aaactatgtt agcgagaatc
     4021 aaaaaaagag gccgggaata tgaacagcta gaacaagatc ctgaactcta ttcctactat
     4081 aaagaactca atcgacgata cgaagagtgg tatgaacagt ttgatatatg tcccaaaata
     4141 gtgatcgatg gggacaaata tgatttcgta gccgatccgg catgtggaga acaaatcgta
     4201 caagaaatca agatgcgggc aaagaaaatg acagaagagg cagatgccca gtcgatccgc
     4261 tacactacaa ataacccgaa agcaaaggga atctttacaa ataaataata taaaaactaa
     4321 gcaaattgct cactcgcaga tatttctctg tgagcagcat cttgcttagt ttttttgatt
     4381 cattgagatc aaatagtgaa ccgattcgta tgataaaatt gccataaatg aaatgtctga
     4441 gtgagggcca tgatcaaaaa taaaatggaa acgatcaaaa tcgaatgggc gccaaacaga
     4501 tgatgacaga aaacaaggac aatagctcct gcgataaagg caagaacgac acttagatag
     4561 tggaaaaatc gatcaaaatc tgctttttct ttagaaatac aataatcata taagttcgtt
     4621 gctacccctt ttaaatttcc agttgtaaat aaattagtgt aggcacgccc gttgatttta
     4681 tcaaaagcta cccactggat cgatgcagta aaagcaatca aaatcgttac taatgcggga
     4741 tgctcatgga cgggtgtcaa actaacaaag aaaaaaatca ctgcttcata atatagaaca
     4801 aacaagcgcc agaaatgttt ttcttttttt ctaaaataat caattagata ctttgttaga
     4861 agaatcccta agaaaaagaa aagggtagac aatacttttt taccaacact tagccattct
     4921 ccgtcaaatg cttggatgat cgatagaatg atattgccag tttgagcaga ggcaaaagca
     4981 ccatattgga tataagtata actgtccatg gctcctccga taaatgtcag caaaagtcct
     5041 attgtacgcc cctcgtgaaa ggaagtctgc atttttttca tcacctttta tgaatatttt
     5101 tgtcttcctt atcttagcat aaacgattga aaaaaaggaa aaattttcag aaaactaaaa
     5161 tcatatcgct tcccattttc atttttagaa aggtctgtta gtataaagaa aaaatagaca
     5221 acgaaacttt aaataaccaa cttgaaataa acaaattcaa agaagaaaga tgggaaggaa
     5281 atcaatcagt tgaatgaaaa agaaattctc cgattacaaa aagaattgaa acgctgggag
     5341 aacaagtata aaaagcttca acaagtcttt cggctacaag aagaaatcat tgctacctat
     5401 gaagcggctt atggcgaagc acccggactt attgaggaag aagtacaagt aaaagaaagt
     5461 gaaggaaaca gctctttctt taaaatgatg gagaaacgtt cctataatcg cttcaatcaa
     5521 gaacaaaaag cagctatcac atatgatatg aagaaacatc ttcgaatcat agcaggagct
     5581 gggagcggca aaacgcaaac aatctgtgca aaagctgttt atctgatgac gcaaaaacaa
     5641 gtagacgaag aacgcatact aatgatcact tttacaagga acgctgcaaa tgaactaaaa
     5701 aaacgagtag ataatttcag ccagagaaaa accgctgtcc atatcggaac attccatagt
     5761 atctttttta ggatatacaa tgaaatttgt cgaaaatttc ctgaagtagc aatgcaagga
     5821 atacaaggag acttttcaaa ggatactgca caaaaagtga atgctgtttt gcaacagctg
     5881 atacgtaaat ataatcttta tatatttgat caatatggag aaaaaacgat tgcttctcgg
     5941 ttagactatt ggcagaatat gaacctttca gtagaagaaa tggttcagtt agttaaagaa
     6001 aaatatgatt ccattgataa gaatgcacga cagccgatat cagaacgact gtatggattg
     6061 ctcacggaac tccaagaaca aaaaagaagc cagcaattgc tagagttcaa tgatgtactc
     6121 caaaatctga aaagcgcttt ggagaatgag gagatccagc attatatcgg ccaaaaatat
     6181 gattatctat tcattgatga atttcaagat actaatccac ttcaatggga aatcgtgaaa
     6241 cggatgacaa aggaaaacag gatcaagttg attgttgttg gagatgatga tcagagtatc
     6301 tatggattca gaggatcaga gccagcatac atcaagaact ttgaaaaaga atatcctacc
     6361 aaaactcttt tcttattgac taattatcgt tctcgggcag ctattgtcca aggagcaaat
     6421 agattgatca gctataataa aaatgatcgg ataccaaagg cgatgattcc agcacaaaaa
     6481 gaagcaggtg ttatggaagc ctatttgttt tctgatacta aagcagaaag tcagtggcta
     6541 atcaaacaaa ttcagacatt tattcaaaaa aatgggaaat ataaggaatc aatcatatta
     6601 taccgatcag cttcacaaac gcagcagctt gtccaatctc tgctgaaaac gaatattcct
     6661 tttgtactgg aagcagatag tccatatgaa ggtatctttg gtatcaagtc tttccaacat
     6721 ttctatcaaa aaattgttca ttggcaaaca gcactgaatc tgcaaaagaa aaatcaggcc
     6781 taccagcaat tgctccggca gctgatggca gactgctacc tgaaaaaaag tgaatgcgat
     6841 caatactttt ctcctaaata tcaaaatata gccgttgcag attatatttt gtcgataagc
     6901 cccaatttaa aatcgaaatc tgcagaaatc cgagagtttg aggaagcgtt gcgtaaaaca
     6961 gaccagcatc aagaaatcta ttcgcttgtt caaagctatc ttcatctgcc acgtatagct
     7021 aaggaaatcg accagagtga tcaagaatgg atacgagaag aagtaaagca gcatgctact
     7081 ttcagaagac tccaagcctt atcgcaagaa acccaagcga cagctaaaga attaaaaacc
     7141 cgcttgattg cttacagaca aggaaaactt gatgcactat gtatccagag cattcacaaa
     7201 agtaaaggtc ttagttaccg aaatgtcttt ttgattggtt gtaacgaagg aagtttgcct
     7261 tataacggag cgacagaaaa gaaagtaatc gactttaatg aaataaaagc agaaccagtg
     7321 acgacgatag aagaagaacg aagactattt tatgttgcga tgactagagc aagaaatcgt
     7381 ctgtatctat gtgtacctca aatgaaaaat tcaagaaaat tgaaagtatc gagatttatc
     7441 aaagaaaccg ggagtcgtgt gaaaaaagga aagtgataga agagagggtg tgacagaagt
     7501 ggtcagtttc aagaaagaag aaggaatgta cgaaaattgc ttctcaaatt tttgtgaaat
     7561 ttcagcttat tttcgaagaa gctatttctg gaacacagtt tattcggaaa ag
//
LOCUS       DARDHA010000133         7600 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000133 DARDHA010000000
VERSION     DARDHA010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.135"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(943..1356)
                     /locus_tag="SDA10_002233"
     CDS             complement(943..1356)
                     /locus_tag="SDA10_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEG4241117.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="SDA10_002234"
     CDS             complement(1812..2297)
                     /locus_tag="SDA10_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241118.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="SDA10_002235"
     CDS             complement(2436..2651)
                     /locus_tag="SDA10_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEG4241119.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="SDA10_002236"
     CDS             2875..3675
                     /locus_tag="SDA10_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG4241120.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="SDA10_002237"
     CDS             3694..5562
                     /locus_tag="SDA10_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEG4241121.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="SDA10_002238"
     CDS             5555..6046
                     /locus_tag="SDA10_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEG4241122.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="SDA10_002239"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="SDA10_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HEG4241123.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="SDA10_002240"
     CDS             6606..>7600
                     /locus_tag="SDA10_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HEG4241124.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1442 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DARDHA010000134         7537 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000134 DARDHA010000000
VERSION     DARDHA010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.136"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(218..895)
                     /gene="nth"
                     /locus_tag="SDA10_002241"
     CDS             complement(218..895)
                     /gene="nth"
                     /locus_tag="SDA10_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEG4241125.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(902..1600)
                     /locus_tag="SDA10_002242"
     CDS             complement(902..1600)
                     /locus_tag="SDA10_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEG4241126.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1868..3052
                     /locus_tag="SDA10_002243"
     CDS             1868..3052
                     /locus_tag="SDA10_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HEG4241127.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3058..3501)
                     /locus_tag="SDA10_002244"
     CDS             complement(3058..3501)
                     /locus_tag="SDA10_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG4241128.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3727..4014)
                     /locus_tag="SDA10_002245"
     CDS             complement(3727..4014)
                     /locus_tag="SDA10_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241129.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4185..5537)
                     /locus_tag="SDA10_002246"
     CDS             complement(4185..5537)
                     /locus_tag="SDA10_002246"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEG4241130.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5810..6256)
                     /locus_tag="SDA10_002247"
     CDS             complement(5810..6256)
                     /locus_tag="SDA10_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG4241131.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6492..7418)
                     /gene="cysK"
                     /locus_tag="SDA10_002248"
     CDS             complement(6492..7418)
                     /gene="cysK"
                     /locus_tag="SDA10_002248"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEG4241132.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2035 a         1543 c         1238 g         2721 t
ORIGIN      
        1 attttatagg tatattggaa ggcattattt tctacatcgg gtattttccc aataaaaata
       61 acaactttta tttttatcgc aataaattgt aataagttac catcctagct actgagtatg
      121 tacatttact ttttcatgca aaaaagataa acgaaacagc ttttctccat aataacaaga
      181 taattcatac atttatttgc aattaaagaa accacgtcta caggcgtggt ttctttttta
      241 gcgccttttc ttttagacgc atccgatttt tcccatcttg acatattgat agcaaaggac
      301 atacctcgca cttgggattt cttgccgtgc aatgatatct accaaaaaaa atcagtgtat
      361 gatgtgtctt tacccataat tcttgtggta ctttccgcat cagtgtttct tctacttcca
      421 tgaccgtagc atctagtttg cagatcctca ggcgttttga gacacgttcc acgtgggtat
      481 ctactgcgat tgctggtatc ccgaatgcat cccccaatac gacatttgct gtctttctgc
      541 ctacaccagg aagagacatc aattcttctc ttgacgttgg cacttgccca tcgaaacgtt
      601 caatcagctg ctgtgcacac gctttgatgt tttttgcttt atttcgatac aaaccaatcg
      661 tttttatttt aagaatgatt tcatcgattg aagcttctgc taacgcatcg ggtgtcggaa
      721 aagaggcaaa caaatctggc gttgctttat tcactgatac atctgtcgcc tgggcactta
      781 agatcactgc aatcaataac tcaaatggat tattgtgctt gagttctcca tgtgcttcgg
      841 gaaacattcc atacatcgtt tctagagctt ccattgtttt ttgtttgctt aacatattcc
      901 gctactcctc caaccaattt ttcagtgaga ctttcggcaa ttcttcttgt tgagctgact
      961 gttctacttc tctttgaaga agctgctgtt ttcttcgttt ttggtcttct tccacttgtt
     1021 gttttgtacg tagatttttt ctttcccacg atagcaaaat gcggtcaacg taattaaaac
     1081 tataagcctg atttaatacg gcttcccgca gtgctagctt caaaatttcc ggctgataat
     1141 ggtcttcttc tagccattgt ccgatccgct ggaactcgat cgaagaaagc gggcgtccaa
     1201 attcttgttc aaataattga tacactgact ggatttcttt ttcttgtgtt ttttcttcgt
     1261 attttttttc ttgtgttttt aaatactcac ctaatagatc atagatgggg taaagattat
     1321 atcgatctgc ttttttaccg ttgacaatcg tcgtttctat cttgataaat ccatttgtta
     1381 cgagacgatc cagtatttga aaaatctgat cttgtttgat tcccatatcc atagcaatca
     1441 tctgcaaatc agggaaagta tctccttcaa gctgtgccat atgcaactgt agaacaaata
     1501 aaaattcttc agattgtaga ccgatcctat gataattttt aagtaaaaga ttagatatga
     1561 ctgtttgtcc agcgcttaag taattttcta aattcaacat gatttctcct ccgctcaatc
     1621 acagtatacg taaaaaaaac atgggaagca agaacaggtt tcttttctat ttacttcact
     1681 gatcggtcag atttacaatg tcgtctactt gatttttgtt tgttcatgtt tttgatgcgt
     1741 atcttcactt ttcggaataa ataaattaag tagatttcgc tgttccttag aatgttgaca
     1801 aggaaacaaa attactatac actatttttg tttccaagtt aacattattg cataaaggag
     1861 tttttacatg tacagtttca caaataaaca ttctaaagtt tttggatacg tctctgtctt
     1921 attcactgga ttaggattta ctatcgtcag tcctgtactt ccgtttttaa cgctgcctta
     1981 ttctcactcc atacatcagc aagctttcta tatcactttg ctcatgtctg tttatgctct
     2041 agctgccttc ttatctgccc ccattctcgg tagcttaagt gatcattttg ggcgtcatcc
     2101 gatccttatt atcagtttat taggatcaag tatcggatat ttgctttttg gtttaggaaa
     2161 ttcgatatgg atgttgttcc ttggtcgtat cattgaagga ttgacggcag gagaaatctc
     2221 caccttatat gcttactttg ccgacatcac ggaacctaat gaaagaacca aagtttttgg
     2281 atggatgggt gctttagtcg gtataggaac aacgctgggt cctattatag ggggtctatt
     2341 agctgagttg gggaattctg ttcctatatt tattggagca ctctttactt tcttaaatgc
     2401 cgtttatggc tacacattta tgctagaaag ccttccaata aaaaaacgtt cagtagattt
     2461 aagtttctcc catgtaagac cttttcacca attacaacaa ttattcaaga tttcatccgt
     2521 tattccttta ttgacagcag gttttgctgt ttggttggca gctggctctc ttcaatctat
     2581 cttttctcaa ttttcgattg acgcctttca gtggaaggct gggttggttg gtttaagctt
     2641 ctctttgatt ggaatcttgg atactgtctc gcaattgttc attatgcctc gattgctcaa
     2701 aaaattttca gaacagcaga tcacaagaat agggatgttc agtgaaatcc tcgcctatct
     2761 attcatcaca ttatctggaa ttcttctact gccgattttg tttttatttg ggattatctg
     2821 ttatggtttc ggtgattcta tttttactcc tgtattcaat ggccaactat ccaattctgt
     2881 ctcagataac cagcaaggtc ttgtaatggg cggtacccaa agtatacaat ctctatcgcg
     2941 cgtcatcggc ccgctaatcg caggacagct ttatgctgca gcaccatttc ttccgacggt
     3001 attcggattt ttgcttatga tttttgccta ttatcactac aataaaaaat agtagcttca
     3061 tttgctactt cgtataaatt ctctgtatcg accgataaaa cgaaatactg tgtcaaattc
     3121 ttcttttgac atattttcaa atacagtagc atctcgctgt acccattttt gatggagttg
     3181 ctcgtgtcta ttccttattt tttgtccgat tggcgtcaat tgaaaataga tttctttttt
     3241 attctcttcg ctttgataag tgtttatgat ccctctagct atcagttttt ttgttagctt
     3301 actgatggct cctctagtca tgtaactagc attcgctaat tttgtcacgt taggatcagg
     3361 gattttcccg atatgttcaa tcacttctag ttcatttaga gtaaatcctg caaattcttt
     3421 ttccatttct ggtttattac gatgaataat ctcattagct aaatccagat attcttctat
     3481 tagcagttgt cttttatcca tttctacacc ttctttttct ttcattatat tgtttttttg
     3541 tttcctattc aacaaagata aaaatatttt gtaggaaaac ccaatctgaa accaagaaaa
     3601 aaaggggttg acaaagcttg tcataacccc tttttctgat taaaagatag taaagagaca
     3661 gtcatcggta attgtgatga ttttttgtca tcacttaagg atgagcggcc ttttcaaaaa
     3721 ctgtctttaa agatcatcat cagtttctag cacatatggc tgaccttcat atgtgtactc
     3781 tgtattcaat gtgccgtcat tttcatctga gtagatgcgt ctactgaaag gaaaacgatg
     3841 agaaacatta tctttggcgt catttgctgg catcgtgtaa tattcctctt gcgtttcatt
     3901 caactgagag agtacttgat ctgccaactc atcaatattg atcgttaatt tcttcgaagc
     3961 acttttataa tcttctaaaa atgcatcttg ttggacaaaa tattctttat tcatcttctt
     4021 tctcaccttt cttttcaaaa tcaaataaag ctgcttgttc ttttatgtaa cagctttatc
     4081 tttgctgctt attgtgacaa aaaattcgaa gaaagacaac taattgtact tttcacaaaa
     4141 aaagacagag ggtcgtgaca aaattgtgtc acaacccttt ttctttattg tgcagtttcg
     4201 atttcgccta gcgctgcttg tgcaagtagg ttcaagtaat tccatgggcg gtcaaaatgt
     4261 ggttggaaga agaaatctga aagtgcgaga tcttctacag tcattttatt ttgaacagct
     4321 aaagacatgg tatttgctga ttgtgtaatg tcgtatttgg acatgaactg agcacctacg
     4381 atacgattcg ttcctttttc atagactagc tccatcatta ctttttcagt agttggcatg
     4441 aactctggac gataattatc ttcaaataca gtcgcttgga cttctagatc gttcaaagtt
     4501 gcgctttctt tggtcacacc tgtcgaaccg atcgtccagc caaataggta aagtcctgaa
     4561 gttccttgag taccacgata cgccatttta tgatctgtca agttatttcc cacaagcaaa
     4621 ccttgacgaa cggcatttgt tgccaaagga atatagtttt gcgtgccact tggattgtag
     4681 ttgactactg cagagtctcc agctgcaaaa atgtctggat tacttgtctg catgtattca
     4741 ttcactttga ttgccccgtt tggcagtgta tctacttttc cttcgatcag ttttgtattt
     4801 ggacggaagc ccacacataa aatgaccata tctgtatcaa atgtatctgt tggtgtagct
     4861 acttgtttca cttttccttc ttcatctgtg ataaattcag taacattttc gcctagcgca
     4921 agagtaacac cgcggtctgt caattctttt tctaagatat ctgtgaatgg tttatctaga
     4981 tatttattca agatgcgatc taatccatca atcaatgtca cttgttttcc agattcgaca
     5041 aaagcttcta ctaattcaat accgatatat ccaccaccaa caattactac acgttttgct
     5101 tctttcgctt gtgcaataat ttcatttgct tgattgtagt ttttgcatag gagtacattt
     5161 ttcgcatcga ttccactaat cggtgggata ataggccatg aaccagttgt catcactaat
     5221 ttatcataag aaaccgtttc tgtttctcct gttttcagat cttttgccgt tactgtttta
     5281 ttttctgtat cgacgtctgt tacatcgtgt tccattttaa ctgttgctcc taatgaagat
     5341 aattcttcag gatttgagta gaacaagcct gctgggtcct ttactactcc gccgacataa
     5401 agtgcgatac cgcaagataa gaaagaaata ttgtcatttc tttcatatac tgtcacttct
     5461 gcttctggat gattttttaa aatacttttt acagctgcag tacctgcatg agtacagcca
     5521 attactacta ctttcattta tggtccctcc gattaacatt ttgtccttcg acaaaactac
     5581 tataacagaa ataaaacgga tacaacagtt taatgcttgt gaattcacaa aggtttgttt
     5641 gtagtttcat tccttttcaa gcataagaac ccaatatcta caagattctt attttaataa
     5701 tattcacttt tatatctaag attgtgaata aaagacaaaa aaataaaaaa gctagcgttt
     5761 tttttacaca gctagctttt tttaggtata acacttgtag aaactgtttt tacatttgcg
     5821 ccttttttgc tgactcttta caatccggac agagtccata caattccaac cgatgttcat
     5881 taatctcaaa atcagtcagt ctgcttgcag ccatttctat atcttcaagc cctgggtaat
     5941 gaaaatcaac aatcttaccg cagttttcac aaattacatg ataatgacgc tttgaactga
     6001 aatcaaatcg gctagaagca tcgccgtact tcatttcttg aacaaatcca atcgaagtaa
     6061 ataagcgaag gttattgtaa acagtcgcaa cactcatatt tggaaaccga tgttcaagtg
     6121 ctttataaat ctcatcagcc gttggatggg aatgatgttc aatcaaatat tctaaaaccg
     6181 cgtaccgttg aggtgtgatt cgaatgttct ctttttttaa cttttcgata acctcttcga
     6241 ctaacttatt ttccatgtaa acaccccacg ttgttattct cgtcttatca tactattttt
     6301 acattttaaa atccacttaa catgaaaaga aaattcaatt ttattgtatt ttaatgaaaa
     6361 aagacgtaaa acagtcgcgt tcactattct acgtctctat tcaaataatt tttctcatca
     6421 taaatttttg tgtgttcttt tcatctttaa aagtttttta cataaaccta tctggagaaa
     6481 aaccaagtgc cttatatctc ttggtaaagg gctgtagata aataacgttc accattgtcg
     6541 ggaaccacag ctaatacctt cttgcctttt cctaattctt ttgcgacttt tagcgcggca
     6601 gcaattgcag cgccagatga gatgcctact aaaatccctt cttttcgccc aacttctcta
     6661 gctgtttcca tcgcttcgtc tccactaata gaaaggactt tatcatacac agaagtatcc
     6721 agtgttttag gaacaaaacc tgtaccgatt ccttggattt tgtgaggtcc cgcttctttc
     6781 ccttctaaaa tagcagattc tgctggttct accccgatta attcgatttt cggatacaca
     6841 cgttttaatt ctcggccagc gccagtgatc gttcctcctg taccgatacc tgcaacaaac
     6901 gcgtccaagc catttactcc aaatgcttga tgtatctcag gaccagtcgt cttctcatgc
     6961 acaagaggat ttgcttcatt ttcaaattgt aatggtaaaa agtagccgtt ttcttttgcc
     7021 aaacgttctg cttctctgat tgatccagaa ataccatccg caccaggagt caaaatcaac
     7081 tctgcgccgt accctttcat caataaacgt ctttcgatac tcatcgtctc aggcatcacg
     7141 atgattactt tatatccttt tgcaactcct accatcgaca atccgattcc tgtatttcca
     7201 gaagtaggtt caatgatagt atcacctggt ttcagtagac catcgtgttc tgctttttct
     7261 atcatactta aagcaatacg atcttttaca cttcctcctg gattaaaaaa ctcaagcttt
     7321 acaaaaacat ctgctgaatc ttctggtacg attttattta atttgacgat tggcgttttg
     7381 ccaatcaatt cggtgactga gttatagata gctgtcatct tcaattcctc cttgtctttg
     7441 ctcttcttac ttatcataat acaatctgtt catttatggc gagtgattcg tctttgacta
     7501 gcaaaaaaaa gacaaagata taccatatat ggtaatt
//
LOCUS       DARDHA010000135         7461 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000135 DARDHA010000000
VERSION     DARDHA010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.137"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(234..1682)
                     /gene="cls"
                     /locus_tag="SDA10_002249"
     CDS             complement(234..1682)
                     /gene="cls"
                     /locus_tag="SDA10_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEG4241133.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1834..2727)
                     /locus_tag="SDA10_002250"
     CDS             complement(1834..2727)
                     /locus_tag="SDA10_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4241134.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2711..3397)
                     /gene="ftsE"
                     /locus_tag="SDA10_002251"
     CDS             complement(2711..3397)
                     /gene="ftsE"
                     /locus_tag="SDA10_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEG4241135.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3502..4603)
                     /gene="prfB"
                     /locus_tag="SDA10_002252"
     CDS             complement(join(3502..4530,4532..4603))
                     /gene="prfB"
                     /locus_tag="SDA10_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEG4241136.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4729..7263)
                     /gene="secA"
                     /locus_tag="SDA10_002253"
     CDS             complement(4729..7263)
                     /gene="secA"
                     /locus_tag="SDA10_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEG4241137.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
BASE COUNT         2007 a         1652 c         1296 g         2506 t
ORIGIN      
        1 aaaaaaaact atctcattaa aagtttctat gctatactgg ctttgaaatg tgtctctttt
       61 aatagcatat attagcttat ttcttgcttt ccccaaaaaa caagagatac cccaaagtta
      121 atttcgctca tgcgagacgc tttctccatt tctgacgtac tctcagaaat ggccccgtta
      181 gaaaaggaca cgagatctcc atctgttgat ggttctcatg tcctttttat gttttaaagg
      241 agcggcgaaa tcaatcttgc taatccttct ttcatcttga tgattattcc tcggttttca
      301 tacatttctt ttgtgagatg cgtagaaatt ttcgagtctt caaaaaaagc ttttcttaca
      361 cgtgaagcca ttcgttcatc ataaaccagt gtattgacct caaagtccaa gcgaaatgaa
      421 cgaacatcga tatttgctga gccaactgaa acaatgccac catcgataat catcgtcttt
      481 gcatgaataa atccattctc atatgtttcg atccttgctc catactcgat tagctctgca
      541 gcgaaagaat acgttgccca gtaaacgaga agatgatctg gtttgttagg tatttggata
      601 tgaactttta cgcctgaaag aagagccaac ttcagtgctt catgaatcga tccatccggt
      661 atgtaataag gcgtttggat caatatctcc cgtttggcta acgaaatcat tttcaaataa
      721 gtcattttga tttgttcatg ttcggtatct ggtccgcttg tcacgatttg aacagctata
      781 tcaccagtag agtgaatgga cggaaaaaat ttgggagaat agcctacttc ttttgtatgc
      841 tgagaattcc agtccatcaa aaaacggttt tgcaaggtat acaccgcttc tccgtaaata
      901 cgcaaatgat tgtctcgcca ataaccaaat ttctttacca atcctagata ttcatttcca
      961 acattgaacc cacctgtata tccgattttc ccatcaataa ctacgatttt acgatgattg
     1021 cgataattga tccgaggatt aatatacgga acaaatagag gaaagaaaaa tgcgacttct
     1081 ccgcctgctt ttttcaaatt ctgaaaaaag ctggaagaaa cttgtgtgga accccatgca
     1141 tcaagaagga ctctgacttt tacccctcgt cttgccgcct ctgtcaatgc atctcgaact
     1201 tcttcaccta atgcatcact gcgataaata taatactgga agtggatatg gtcttctgcc
     1261 tgcttgatat cttcaatcag cgcatcgaat ttttcacgac catctgtaaa taaagtgatt
     1321 tcatttttag tcgtatagag cgtactttca aataccgtga gcatatagat caattgtttc
     1381 acatctactt gtccagtagg cggatgagga taaagccctc gcatcaatgc ctgtttgtct
     1441 tcttcaattt ccaggttctt accgattttc ccttggatct tcaaatcaaa gattttctct
     1501 ttggaaatcc ctcttcctaa aaaaatatat aaaataaatc ctacaactgg aatgaacatc
     1561 aaaacgagca cccatgccca tgtttgagca gtttcctttc tttctcgaaa aacgatgata
     1621 aaggaaagca aaatattcat aatcaaaagg atcgtaaaaa aattgtcaac gataattttc
     1681 acggcttcac ctcttcttct accaacttaa gaatacccaa gttgtttttt aaagtaaagg
     1741 gaaaaaagcc gcttccttct attctcctag ccaataaaaa aagaaagctg gaacgaaact
     1801 gtccaacttt ctcattttat ttatattgtg gtattagaat ttcaagaaac gtctcataga
     1861 aatgatcgag ccaagtgaac caatgatcac accaatgccg accattaaaa gattgatttt
     1921 ccaaacgata ttttgtggat gaatcaatga ataatgggaa cgtaataatg atggattgat
     1981 caattggaaa aaccatgcat aaccaaatgt gatgatcaaa atcggaagaa tcgcaccgat
     2041 cagaccgatc cagccacctt caaggaaaaa cggccagcga atatatccat tttttgcacc
     2101 gactaaacgc atgatttgaa tttcacgctg tctagacaaa atcgtgatcc gaatcgtgtt
     2161 cgaaatgaga aacatcgcta cgaacagcag caatgccgct gcaactaatc cccatgtccg
     2221 aactgcacca gcgatttgga aaatccgatc tgaggaagct ccaccgtaat cagaacgaga
     2281 aacattcttc aaatctgctg cttcttctgc tacttgtttt gtataagaag gatcagtagc
     2341 acttactaca tacacatcat ataacgggtt attgtcacct tcaaacaatt tccaggaatc
     2401 acccatttcg ttttggattt ttttcaattc ctgttctttg cttgaaaatt caactttttt
     2461 aacatgatct aagtcattca attctttttc tagctttttc atctcttcgt cttttgtacc
     2521 gatatctacg aatacagaga catcaacgtt tccttctata tcttgagcta atttagttgc
     2581 gttcatgata actgccatga atacaccgac taaagtcaat gtgatcgtta ccgcactgac
     2641 tgaagcaact gtcatccagc cgttacgttt caaactcttg atactttcta ataaatggcg
     2701 gaaaaaggtt ctaatcatcg tatccatatt ccccttccgc ttgatctcga ataatgcggc
     2761 cgttttcgat agcaattact cggtgccgaa tcgtatttac gatcgtactg ttatgtgtag
     2821 ccatcacgac agtagttcct tgggcattga tccgatctaa taatttcatg atttcccatg
     2881 agttttctgg atccagattt cctgtcggtt catccgcgat aagtacttta ggcgtattca
     2941 ctattgcccg agcaatagag acacgttgct gttccccacc ggataattcg ctaggaaaga
     3001 ctcgaacttt gtgtttcaaa ccaacaaggt ccagcacttc cattactcgt tttttgatat
     3061 cccgaggttt tctgccgata acttgcatag cgtacgcaac attttcatat actgtttttc
     3121 ttggtaataa tttatagtct tggaacacca cgccgatttc tctgcgtaga taaggaactt
     3181 cgcggttttt gattgctatt agatcatgac ctgcgacgtt caaagttcct tttgaagctt
     3241 tttcttctcg atacatcaat ttgatgaatg tagatttacc ggctccagac ggaccaacca
     3301 catagacaaa ctctccttgt tcgatgacaa ttgagagatt gcggatagcg gtcgtaccat
     3361 ttgagtactt cttcattaca tccttcattt caatcatggc gttctctcca tatctttttc
     3421 agtctttagc tattatagca tggcaatgtt tcaacatgct tgcaaaatct tacaatttta
     3481 tttcaattta acaatgggtt cttattgtag atgcattttt aaatagccgt caataaataa
     3541 atcaaggtca ccgtccataa ctgcttggac atttcctgtc tctacgtttg tccgatggtc
     3601 ttttaccata gaatatggat ggaaaacata agaacggatc tgtgaacccc agccgatttc
     3661 tttttgttct cctcgaagtg cagctgcttc ttgttctttc ttttccactt ctaattgata
     3721 aagcttagct tttaacatcc ccatcgcttg ctcacggttt ttcaattgtg aacgttgtgc
     3781 ctgactagct actactgttc ctgtaggcaa gtgagtgatc cgtacagcag attctgtttt
     3841 attgatgtgc tgaccacctg caccacttgc tcggtacgta tcgattttca aatcatctgg
     3901 attgatttct atatcgatcg tttcatcaag ctctggcatc acatctaccg aacaaaaaga
     3961 agtatgacgt cgtttcgctg agtcaaaagg cgagatacgt acaaggcgat gaacaccttt
     4021 ttctgatttc agataaccaa atgcgttgtg tcctttgatc agtagggtca cactcttgat
     4081 tcctgcttca tcacctgctt gataatctag tgtctctact tgatacccat gctgctctgc
     4141 ccagcgtgta tacatacgta aaagcatact gccccaatct tgggactccg ttccgccagc
     4201 ccctggatgc aattcaacga tggcgttgtt tcgatcataa ggaccatcta acaacataga
     4261 taattcatat gtcttcattt tttcatctag cgcattgata cgtgtttctg cttcttcttg
     4321 catggctgca tcaggttctt cctgcaacat ctccaccatc aattcgattt cttccagttc
     4381 ttcagcaaga gaatggaatt gatcataagt ttctttattt gcattgtttt cactgatcac
     4441 tttttgagca gcttcgctat catcccaaaa accaggttca gccatttttg cttctgcttc
     4501 cgcaatgtct tcttccagct ggtctaagtc aaagagacct cctgaagctt gtgacctttt
     4561 cctgcatttc tgtcaggaga tttcgaattt cggatatttc catgtttttt ccttctttca
     4621 ctatactttt ttcattatat ctagtctaac agaatcagca aataacagaa agaaggtgag
     4681 acgaattctt aaaaaaagaa tttgtctcac caatcctgct gtcagaaatt agtttgcatt
     4741 ttttccatga cagttcttat attttttccc gcttccacaa ggacaaggat catttcttcc
     4801 gaccttgttc acatggattg gctgtttttt cgcgctagta ttactatttt ctttggcatc
     4861 tccgtcttct gagtgagaag ctgttccttg agaaacctgt tcacgctgta cgttttgacg
     4921 gatctcagct ttcataaaga ttcgagtgac ttcatactcg atggcgccta ccatatcttc
     4981 aaacatctta tagccttctg tttgatattc gactaatgga ttgttctgtc cataagcacg
     5041 taagccgatt gactgacgta attgatccat tgcatcaata tggtctgtcc atttagagtc
     5101 cacaacacgc aagatcacga ctttttcaaa ttccaacagt tgctctggac catttagttg
     5161 agccgctttg gtattaaata cttcttgtgc tcgttcgttt agatactctt tgatctcctg
     5221 agctgttttt ccttcgatat ctgcaagagt aatactatct tcatggacaa gagtactgcc
     5281 ggcaaaatcg atgatacttt ccaaattcca attttctttc tctaattgtg tatgcgcatc
     5341 tactacacga ctgattgtcc gtttgaccat gttcattagt ggcttggata agcttttttc
     5401 ttccatgatt acttcttgtc gctgtttata gattacttca cgttgttcac gcatcacatc
     5461 atcgtattgt aagacgtttt tacgtgtgtc atagttattt ccttctacac gtttttgtgc
     5521 agattctact tgtttcgtca gcatcttgct ttggataaca gcatcttcct caccgatctt
     5581 catccgatct aagaaagatt tgattcgttc tgaaccgaat cgtttcatca aatcatcttc
     5641 caatgacaag tagaactgtg acataccagg gtctccttga cgtcctgcac gtcctcgaag
     5701 ctggttatcg atacgacgag attcatgtcg ttctgtcccg ataactgcta gaccaccaag
     5761 ttctcttacc ccaagtccta acttgatatc tgttccccga ccagccatgt tggtagcaat
     5821 cgtcacagca cctttttgac cagcattcat gatgatctct gcttctttaa agtgattttt
     5881 cgcattcagc acttcatgag gaatacgttc ttgattcagc atatctgata ataactcaga
     5941 tgtttcaacc gcaacagttc cgactaacac aggttgacct ttgcggtagc gatctttgat
     6001 atcttgcacg acagcgtgga acttgctttg taatgttggg taaagcaaat ctggacgatc
     6061 atcacggata accggacgat ttgtcggaat ctggtatact tggatgttat aaatttctct
     6121 gaattcttct tcttctgttt tagctgtacc agtcattccc gcaagtttct tgtacatacg
     6181 gaaatagttc tggaaagtga tcgtcgccat ggttttcgtt tcgtcttcga tctctacgcc
     6241 ttctttagct tcgattgctt ggtgaagtcc atctgaataa cggcgtccat ccatgatccg
     6301 accagtgaac tggtcaacaa tcagtacttt tccgtcttgg acaacataat cgatatctaa
     6361 aagcatgata tagttggctc tcaatgcttg atccaagtga tgggtcaatg cagtattttc
     6421 aatgtcgtat aagttatcca aaccgaatgt ttcttctgct ttttcgatcc ctgcttcggt
     6481 taaactgatt gtttttgatt gtacatcgat tttataatct tcttcttctt tcaaacgttt
     6541 cacgaagttg tctgctcttg tatataaagc tgtggatttt tctgcttgcc ctgaaatgat
     6601 cagcggcgtt ctcgcttcat caatcaagat tgagtcaact tcatcgacaa tggcataatt
     6661 cagcggacgt tgtaccattt gatggcggta aacgaccata ttgtctctca agtagtcaaa
     6721 tcctaattca ttattcgtac tataagtgat atcacaatta taagcctcgc gtttttcatc
     6781 cgaagatttt gagttgatat tcaaccccac tgttaatcct aagaaattat aaagttcgcc
     6841 catttccgtc gagtcacggg ttgctaaata ttcattgacg gtaactacgt gtaccccttc
     6901 gccagtcaga gcatttagat atactggcat cgttgcggtc aaggttttac cttcaccagt
     6961 acgcatttca gggatatttc catcgtgaag cacgattcca cccatcaatt gtacgtggtg
     7021 tggatataac cccaatacac gttttgctgc ttctcggacg actgcaaaag cttccggaag
     7081 taattgatct aatgtttctc ctttttggta tctgcccttg aattcagcag tttttgcctg
     7141 taattgttca tcagttaatg ctgccatttg gtcagcgtat gcttcaactt tttttgcaat
     7201 gctatctaaa cgttttagtt ctttcttatc gttttcgata agtgaacgaa tcaaattggc
     7261 catgcgttgt gtaatctcct tttccttctg tgtattatcc agactatgta gactttactt
     7321 ttcattttat cattagttat agataattga aatatcttaa gcgtattaaa gtaagaactt
     7381 cataatttat tcatatttca tctaaaagag gaggaaactc aaaaaaactt gcggcagctg
     7441 tcagtagctg ctgcaagttt t
//
LOCUS       DARDHA010000136         7437 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000136 DARDHA010000000
VERSION     DARDHA010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.138"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="SDA10_002254"
     CDS             complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="SDA10_002254"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEG4241138.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="SDA10_002255"
     CDS             complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="SDA10_002255"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEG4241139.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2303..2965)
                     /locus_tag="SDA10_002256"
     CDS             complement(2303..2965)
                     /locus_tag="SDA10_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4241140.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3149..4795
                     /locus_tag="SDA10_002257"
     CDS             3149..4795
                     /locus_tag="SDA10_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEG4241141.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4786..5868
                     /gene="uxuA"
                     /locus_tag="SDA10_002258"
     CDS             4786..5868
                     /gene="uxuA"
                     /locus_tag="SDA10_002258"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG4241142.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5976..7437)
                     /locus_tag="SDA10_002259"
                     /pseudo
     CDS             complement(5976..7437)
                     /locus_tag="SDA10_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2332 a         1363 c         1375 g         2367 t
ORIGIN      
        1 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
       61 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
      121 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
      181 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
      241 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
      301 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
      361 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
      421 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
      481 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
      541 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
      601 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
      661 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
      721 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
      781 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
      841 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
      901 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
      961 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     1021 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     1081 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     1141 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     1201 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     1261 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     1321 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     1381 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     1441 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     1501 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     1561 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     1621 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     1681 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     1741 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     1801 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     1861 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     1921 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     1981 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     2041 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     2101 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     2161 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     2221 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     2281 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     2341 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     2401 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     2461 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     2521 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     2581 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     2641 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     2701 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     2761 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     2821 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
     2881 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
     2941 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
     3001 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
     3061 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
     3121 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
     3181 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
     3241 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
     3301 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
     3361 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
     3421 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
     3481 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
     3541 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
     3601 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
     3661 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
     3721 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
     3781 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
     3841 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
     3901 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
     3961 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
     4021 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
     4081 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
     4141 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
     4201 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
     4261 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
     4321 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
     4381 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
     4441 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
     4501 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
     4561 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
     4621 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
     4681 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
     4741 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
     4801 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
     4861 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
     4921 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
     4981 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
     5041 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
     5101 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
     5161 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
     5221 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
     5281 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
     5341 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
     5401 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
     5461 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
     5521 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
     5581 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
     5641 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
     5701 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
     5761 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
     5821 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
     5881 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
     5941 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
     6001 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
     6061 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
     6121 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
     6181 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
     6241 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
     6301 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
     6361 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
     6421 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
     6481 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
     6541 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
     6601 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
     6661 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
     6721 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata
     6781 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
     6841 ggaaaattct ctttacatat ttttaataga aaatataatt tatctatcat accgctattt
     6901 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
     6961 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
     7021 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
     7081 atagacaaac cgtactatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
     7141 gaggcactat agtttgtttg ctgactgaaa ctgataatcg aatttgtctg acagctgaac
     7201 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
     7261 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
     7321 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
     7381 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaag
//
LOCUS       DARDHA010000137         7433 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000137 DARDHA010000000
VERSION     DARDHA010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.139"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(14..706)
                     /locus_tag="SDA10_002260"
     CDS             complement(14..706)
                     /locus_tag="SDA10_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG4241143.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1143..1712)
                     /locus_tag="SDA10_002261"
     CDS             complement(1143..1712)
                     /locus_tag="SDA10_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG4241144.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="SDA10_002262"
     CDS             complement(1786..2109)
                     /locus_tag="SDA10_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241145.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="SDA10_002263"
     CDS             complement(2263..3075)
                     /locus_tag="SDA10_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241146.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="SDA10_002264"
     CDS             3429..4277
                     /locus_tag="SDA10_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEG4241147.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="SDA10_002265"
     CDS             4422..5363
                     /locus_tag="SDA10_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEG4241148.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7433
                     /locus_tag="SDA10_002266"
                     /pseudo
     CDS             5353..7433
                     /locus_tag="SDA10_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2191 a         1479 c         1339 g         2424 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa att
//
LOCUS       DARDHA010000138         7281 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000138 DARDHA010000000
VERSION     DARDHA010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.140"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            68..2677
                     /gene="clpB"
                     /locus_tag="SDA10_002267"
     CDS             68..2677
                     /gene="clpB"
                     /locus_tag="SDA10_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEG4241149.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2828..3514)
                     /locus_tag="SDA10_002268"
     CDS             complement(2828..3514)
                     /locus_tag="SDA10_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241150.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3492..3986)
                     /locus_tag="SDA10_002269"
     CDS             complement(3492..3986)
                     /locus_tag="SDA10_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEG4241151.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4006..5226)
                     /locus_tag="SDA10_002270"
     CDS             complement(4006..5226)
                     /locus_tag="SDA10_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4241152.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5373..7208
                     /gene="lepA"
                     /locus_tag="SDA10_002271"
     CDS             5373..7208
                     /gene="lepA"
                     /locus_tag="SDA10_002271"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEG4241153.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT         2394 a         1393 c         1496 g         1998 t
ORIGIN      
        1 atataatagt ttttgtaaag atcaaagaaa taagtgatct tacctttaat taggagggta
       61 tacctgtatg aatattgaga aaatgactac gacgcttcaa gaagcgattg ctgaagctca
      121 acagattgcg gtcactcgaa aacatcaaga tatcgacatt gcccatgtat ggaaaatctt
      181 tttacaatct aatcattttg gtcgaaattt ctatacggat gccggattgg atgtcgaatc
      241 ttttgaacat gaaattgatc gtctactgga tgagtatcca gttgtttctg gtggaaacgt
      301 ccaatatgga caaaacctca gccagaattt ctttcgatta ttgaatgaag cagaccaaat
      361 acgtgaaagc ttcggcgacg agtttctttc aacggaagtc gttattttag cgttgatgaa
      421 attaaaaaat tatccgttaa ctgtttattt gaataaaaac ggattatctg aaaaagaatt
      481 gcgtaaaaac atcgaagaga tgagaggagg agaaagagtg acctcacaaa accaagaaga
      541 acaatacaaa gcattagaaa aatacggcgt tgatttagta cagcaagtca aaagcggaaa
      601 aatggatcca atcatcggtc gtgatgaaga aatccgtgat gtcatccgta ttctttcacg
      661 aaaaacaaaa aataatcctg ttctgatagg agaacccggt gtagggaaaa cggcaatcgt
      721 tgaagggctt gctcaacgta tcgtacgcaa agatgtgcct gaaaatttaa aagataaaac
      781 gattttttca ttagatatgg gtgcattgat cgctggagca aaattccgtg gtgaatttga
      841 agaaagacta aaagcagtct tgaaagaagt caaaaaaagt gatggacgta tcctcttgtt
      901 catcgatgaa atccacaata tcgtaggggc aggaaaaaca gaaggaagta tggatgccgg
      961 aaatctgttg aaaccaatgc ttgctcgcgg tgaattacat ttgatcggtg ccacaacttt
     1021 agatgagtac cgtcaatata tggaaaaaga caaagcgttg gaacgtcgct ttcaaaaggt
     1081 attagtcaaa gaaccaacag tggaagatac gatcagtatt ctgcgtggat taaaagaacg
     1141 ttttgagatc caccatggtg ttaacatcca tgacaacgca ttagtagcag cagctacttt
     1201 atctgaccgt tatattactg atcggttctt accggacaaa gcaatcgatt tgatcgatga
     1261 agccagtgca acgattcggg tagaaatgaa ctctatgcca acggaattag atcaagttac
     1321 tagacgtttg atgcagttgg aaatcgaaga agctgcactg aaaaaagaat cagatgatgc
     1381 aagtaaaaaa cgcttgaaaa atttgcaaga agaattagct gaacttcgtg aagaagcaaa
     1441 tgcgatgaag atgcagtggg aaacagaaaa agaagaagtc aattctgttt cagcaaaacg
     1501 tgctgaaatc gataaagcaa aacacgaatt agaagatgca gaaaataact atgatttaga
     1561 acgcgctgct gtattgcgtc atggtacgat acctcaatta gaaaaagaat tgaaggaatt
     1621 agaagctaaa gcaaaagaca gcgagattaa gatggttcaa gagtccgtta ccgaaaatga
     1681 gattgctcaa gtcgttggca gattaacggg tatccccgta acgaaacttg tagaaggtga
     1741 acgagaaaaa ctaattaaat tgaatgaaac acttcataaa cgagtgattg gtcaagatga
     1801 agcagttgat gcggtcagtg acgcggtaat tcgttcacgg gctggattgc aagacccaaa
     1861 tcgtcctcta ggttctttcc ttttcctagg accaactggt gtcggaaaaa ccgaattagc
     1921 gaaagcgtta gctgagaact tgtttgactc agaagatcat atggtacgga tcgatatgag
     1981 tgaatatatg gaaaaacatg ctgtgtctcg tctagtcgga gctcctccag gctatgtagg
     2041 ttacgaagaa ggtggtcaat taacggaagc tgtcagacga aatccttata caatcgtctt
     2101 gttggatgaa atcgaaaaag cgcatcccga tgtcttcaat atcttgttac aagtcctaga
     2161 tgacggacgc ttgactgatt caaaagggcg agtagtagat ttcaaaaata cggttctgat
     2221 catgaccagc aatatcggtt cacagttact gctggaaggc gtgactgctg atggaacgat
     2281 tccagaagca gtggctgaac aagtcaacac gctattacgt ggaaacttca aaccagaatt
     2341 cttgaaccga atcgacgaca cgattttatt tacaccattg agcctagaca acgtgaaagg
     2401 aatcgttgac aaaatggtgg cgcaattggc acaacgtctg gaacatcaag aaatcctgct
     2461 gacaatcagt gatgaagcca aaacgtggat tgctgaaaat gcttatgaac cagcatatgg
     2521 tgcaagacca ttgaaacgat ttatcactaa ggaagtagaa acaccactag ctaaagaaat
     2581 cgttgcagga catgttatgc ctaaatcaaa agtaacgatt actttacttg atggacaact
     2641 tcatttcaaa acagaagagc tagaagaaat cgtctaatct tccattgaga ctataaaaga
     2701 aaaaaggggt ctgacacaac tagtgtcaga cctccttttt tcaaataaac agtgttccag
     2761 aagtagtttc ttcgaaaaat aagccgaaat ttcttgaagc tgcccgcttc tgtcacaacc
     2821 tcttttatta ggtatttttc actttttcta atttataact ttcaaagtaa cgctcatctt
     2881 ttttactttg agaccatgca aacaatccca ttaaggcagc acctatcgta tttgtcagca
     2941 agtcgcccat cgtatccatc aatgctgctc gtccgacaaa cagtgtgcca tcagaagcag
     3001 caaaacgttg aagattcatg cctaagaact ggtcgcataa gaattcccag aactcccaaa
     3061 acacgccgca aagtccagca aacgcaaagc cgaataagag gaacaaccaa ggactggttt
     3121 ttgatatctc agcatctttc atcaagtaac caatcaatcc atagccaaga gctgtcagga
     3181 ccattggact caccgcatgt aagattttgt cccaaaaaga gatgatactg atcaaatgca
     3241 tccctgtgcc taaaaagaca gagatgaaca agaaaaacca gtaaaacaaa acgattgctg
     3301 ctggtatttt aagtttgaaa actttcaaaa tgacttgtgg catgaaaatc aatgcaatcc
     3361 ctaataaaga ttccacgata aatgtgattc cttgagaagt cgaatatttt tgtgtgataa
     3421 attcataaaa attatagcag acaacgaata caccaaaagc aagtaaagct atttgcacct
     3481 ttttgatttc tttaatcact gaaagtccct ccatctaaaa atctttttac ttctgaaagc
     3541 aactcaattt ctgaagaaac cacttttccg ttcaatttga tcaaacctat tgtatataag
     3601 ttcaggtagt gaaattgatt ttcagctgtt tcttgtagtg cagcgatttt tttcgggtgg
     3661 gttccgcctt gctgtcgagt atcagtatat aagccgatca ctgggatgcc tttcgcataa
     3721 gccacaccga tttccgatgc taccccggca tcgatcgtca gcccatctag taaagcaatc
     3781 attaaatcac tagccaacac tttttctgtg tcagcaagag caatcattcg actgtctgca
     3841 taagcagttt tgtcattgat tgcttcattt tcttgaggaa gatagatcga aagctcagaa
     3901 gagagctctc tgatttcttt aactaactgt tggttatata acaaatcact tttagcaaac
     3961 attggtgcgg caaaataaat tttcatctaa acatcatcct ttttatcaaa aataatcgct
     4021 aaagtaagct tccatgtttg gaatgatacg ctctagtgac ttcagtgttt cttcatctgt
     4081 ttctaaccgt tcgattcttg ccaaataaga agcacggcga taattcatca tcaagcaagt
     4141 caatgtttgg atatccagtc gaacggattt tcctataggt tcatttaaga cacggacttg
     4201 tccctgttca tcccatgtta aagcgaagac accattattc caaggagcga caggatcttc
     4261 aatgatgaaa tgaaaagcat cagctgttcc aacaaacgga aaccgttgaa ggaattcttt
     4321 tacatcaacg atccgagcca taaaatatgg ttcgatctgc tctttgatct gactatcttc
     4381 aatcaaaaat gctaaaggct catttttata aatatctcca tgtacccagt agatcatgga
     4441 aaaatgtgcg gaaataaagt tccataagcc atttcgagct tcttgatcaa gatagaacat
     4501 ttcttttacg tgaaatattt cttcagctac ccagtagaac agaacacctc ttggttcatg
     4561 gttgctatcg tagtagacag cggctgtgcg ctcatcttca ttttcgaaac gccaatactc
     4621 ttcccaatga aaatcactgc ggaataaagc accatggttt tgttgggcaa atttgtcata
     4681 aacggaaaat acgtctggat gatcaactgg taatcgctca accataccac ttactgggat
     4741 ttgtttaggt agctgtgtat ctctgatttt aaaggaaagt ttatcagaca tgatttccca
     4801 tcctttacgc cgatagtagg ggatactgta aggatacaaa taagagatcc attgcttatt
     4861 cttgcgcata ttatccaatg caacgataat tagatcttgc atcaatccat ggttagcata
     4921 ttctggataa gttccaactc cagtcacacc acccatttta taacgtgcac cgtgaatatt
     4981 gacttcacaa ggatagatag cgatttgaga gatcagttta ttttcgtgga accatccgaa
     5041 cactttcgat acttccaaaa tcggtttttt ggaacggata aaagctcgtt tattctcaaa
     5101 cccgctttcc tcgatgtctg cttcagtcac ttggaacaca taggaaagca attcattgaa
     5161 ttgatcgata tgttcttgag tgaccggttt taattcaaga ttttctctaa aatctttttg
     5221 atccattttt tcatctccat ttattagctt gataacagta tagcaaaatt ctacaaaaaa
     5281 aacttctata gctgactaat taaaaactct ttaaattact gcttttcgtt ggtataatgg
     5341 aaagtgctga ttatatgaaa agaggagacg ctatggatat aaagaaaatg aaagaacgcc
     5401 aagagcagat tcgtaatttt tcgatcatcg ctcatatcga ccatgggaaa tctacattgg
     5461 ccgatcgtat tttagaaatg acgcacactg tgacgtctcg cgaaatgcaa gatcagctgt
     5521 tagactcgat ggatttggaa cgtgaacgtg gaatcacgat taaattaaat gctgtagaac
     5581 tcaattacac agcgaaaaat ggagaaacat atatctttca cttgattgac accccagggc
     5641 acgtcgactt cacctatgaa gtctctcgaa gccttgctgc ctgtgagggt gccgttttag
     5701 tggtcgatgc agcacaagga atcgaagcac aaacattggc caatgtttat ttagcgttag
     5761 acaatgattt ggagatcctg ccagtcatca ataaaatcga cctacccgca gcagatcccg
     5821 aacgtgtccg tcaagaaatc gaagacgtaa tcggaatcga tgctagtgaa gctgtattag
     5881 ccagtgccaa atcagggatt gggattgaag atattctcga gcagatcgtg gaatatgttc
     5941 ctgcaccaag tggagactta gatgcaccgc taaaagcttt gatctttgac tctatttatg
     6001 atagttaccg cggtgttgtt ctaaatgtcc ggatcacgga cggtatggtc aaacccggag
     6061 acaaaatcaa actgatgagt aatggcaaaa catttgatgt tacagaggtc ggggtcttct
     6121 cgccaaaagc agttgctaga gactttttga tggtcggtga tgtcggttat atcacagcaa
     6181 gtatcaaaac tgtccaagat acacgagtag gggatacagt aacgttagca gacaatcctg
     6241 ccgctgaagc tttaccaggt taccgaaaaa tgaatccgat ggtttattgt ggtctttatc
     6301 caattgatac ttctcgttac aacgatttgc gggaagcttt agaaaaactg caattgaatg
     6361 atgctgcttt acaattcgaa ccagaaacct ctcaagcatt aggttttggg ttccgctgtg
     6421 gattcctcgg acttcttcat atggatgtcg tccaagaacg cttggaacgg gaattcaatc
     6481 tagaattgat cacgactgca ccatccgtta tttaccatgt taataaaacg gatggtacga
     6541 ctgctacagt agataacccg gcagattttc ctgaacctgt aacgattcag gatgtagaag
     6601 aaccattcgt caaagcacag atcatggtcc caaatgattt tgtcggtgca gtaatggaac
     6661 tttctcaacg aaaacgggga gaattcatta cgatggatta tttagatgat taccgagtaa
     6721 atgtcgtcta taatattccg ctttctgaaa ttgtctttga ctttttcgac aaattgaaat
     6781 caagcacgaa aggctatgcc tcattggatt atgaaatgtc tggttatcaa aaaagcaagt
     6841 tagtgaagat ggacatcttg ctgaatggag aaaaagtcga tgcattaagc tttatcgttc
     6901 accgagactt tgcctatgaa cgtggaaaag caatcgttga aaaactgaaa aaactaatcc
     6961 cacgccaaca atttgaggtg ccaatccaag cagctattgg acaaaaaatc gttgcccgtt
     7021 cagacatcaa agccttgcgt aaaaacgtct tggctaaatg ttatggtgga gatgtttctc
     7081 gtaaacgtaa actcttagag aaacaaaaag aagggaagaa gcggatgaaa caaatcggct
     7141 ccgtcgaagt tccgcaagaa gccttcatgg ccgttctgaa aatggacgag gacgaaccga
     7201 agaaatagca agggtttgtt tgaaaacagt gtaaaaaata aaccatgttt acatggattt
     7261 gccaccggtt tgccaccggt g
//
LOCUS       DARDHA010000139         7109 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000139 DARDHA010000000
VERSION     DARDHA010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.141"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            236..955
                     /gene="atpB"
                     /locus_tag="SDA10_002272"
     CDS             236..955
                     /gene="atpB"
                     /locus_tag="SDA10_002272"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEG4241154.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            1008..1223
                     /gene="atpE"
                     /locus_tag="SDA10_002273"
     CDS             1008..1223
                     /gene="atpE"
                     /locus_tag="SDA10_002273"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEG4241155.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            1314..1838
                     /gene="atpF"
                     /locus_tag="SDA10_002274"
     CDS             1314..1838
                     /gene="atpF"
                     /locus_tag="SDA10_002274"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEG4241156.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            1825..2367
                     /locus_tag="SDA10_002275"
     CDS             1825..2367
                     /locus_tag="SDA10_002275"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEG4241157.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            2390..3946
                     /locus_tag="SDA10_002276"
     CDS             2390..3946
                     /locus_tag="SDA10_002276"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEG4241158.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            3960..4862
                     /locus_tag="SDA10_002277"
     CDS             3960..4862
                     /locus_tag="SDA10_002277"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEG4241159.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            4885..6291
                     /gene="atpD"
                     /locus_tag="SDA10_002278"
     CDS             4885..6291
                     /gene="atpD"
                     /locus_tag="SDA10_002278"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEG4241160.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            6305..6727
                     /locus_tag="SDA10_002279"
     CDS             6305..6727
                     /locus_tag="SDA10_002279"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEG4241161.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         2305 a         1276 c         1542 g         1986 t
ORIGIN      
        1 taatctgata aaacatgatt atcataggtt ttttcttaga tcaggagcac cgtgtataaa
       61 gaaaaattcg cttcgttttt gactgaaata aaaaagtgtc taattttcat gaaaatgttt
      121 tgaattttct gtttcatggt ggtatgatac cgtgtggtta gaaaccgatt ggaaaaaacc
      181 agatacgcac atgaattgat tttatgtgaa tttcggggaa aagaggtgaa atggattgga
      241 tgaacgctcg ctaacgttcc acattggacc aatttggttt gatggtacag tttgtataat
      301 ggtgttactg acctgtttga ttgttttctt cttagcttac ttttttacaa gaaatcttaa
      361 gttgaaacca acaggcaaac aaaatgcgat agaatgggtc attgatttta gtcgaggaat
      421 cgtaacagat aatctgccta gaaaagaatt gagcaatttc catttgttgg catttacatt
      481 gtttttattt gtttttgttg caaacaacat cggtttagta acaaaaatcg ttttacctaa
      541 cgaaacaaca ctttggaaaa gcccgacagc agacccgttt gttactttaa ctttggcatt
      601 tataatgatc ttacttactc acttatttgg ggtgaaaaag ttaggcttca aaggatattt
      661 cgtaaattcg ttcttgaaac catatagttt tatgtttccg atgaaactga ttgaagaatt
      721 tacaaactta ttaacacttg ctttacgtct ttatgggaat atttatgcgg gggaagtttt
      781 gctaaccttg attgcaaaaa tggtaataag cttaggctgg ttctcattgc cgttagcaat
      841 tccattagag atggtctgga tcgcattctc cttgtttatc ggaagcatcc aagcatttgt
      901 cttcgttact ttatctatgg tttatatgag ccataagatt gaagtggaag aataatattc
      961 gttttaaaaa caaactacta tttatctaat ttaggaggaa aacaattatg aattatatcg
     1021 cagcagcaat cgcaatcatg ggagcagcaa tcggagccgg ttatggtaac ggtcaagtta
     1081 tctctaaaac aatcgaatca atggctcgcc aacctgaaat gtctggtcaa ttccgtacaa
     1141 caatgtttat cggggtagct ctagtcgaag ctgttcctat cttaggggta gttgtcgcct
     1201 tgattctagt ttttggtgtt taattgaatc agtattaagg cagaaacaat ttggtttgtt
     1261 actgcttttt tacatgaaaa ctagaagtac ttcaaagaaa ggaaggctcg cctatgctga
     1321 atcaattggc aattgcagaa gttggcaatg cgatgttagg aaatgtcatc gttgtcagcg
     1381 gctcattttt gatactactg gctctcttga agcattttgc ttggggacca atcagcgata
     1441 ttttgaaaaa acgtgaagac aagatcgcca atgatttgga ttctgcagaa caatctcgca
     1501 tcaactcagc gaaaatggaa caagaacgcg aacaacaatt gttagcctct cgttctgatg
     1561 cagctgatat catcaaaaat gcgaaagaaa gtggagaatt aagccgccaa aatattttga
     1621 aggatgctca agaagaagca gctcgtctaa aaagtaaagc ccaagctgat atcactgtag
     1681 aacgtgattc agcgctgaac tctgtaaaag acgatgttgc agaactatct cttcaaatcg
     1741 cggaaaaaat cttgaacaaa gaattatctc cagaaatgca cgaatcatta atcaatcaat
     1801 atatcgaagg tctaggttct tcaaatgaaa ctagataaat atacagtagg aagacgttac
     1861 gggaaagctt tatttgaact ggcgatcgat tcaaataaag cagaagaaat ttatcaagat
     1921 ctcgtaagct tacgtcaaat ctatgaacaa gtacctggat taggtaatat gttaagtgat
     1981 gttcgtctag aacctcatga aaaacgagaa atcatggaca agcttgttag tggctatgat
     2041 ggaatcgtta aaaacttttt agaagttgtt tatgcgtata accgaatgga tgatcttttg
     2101 ttcatgatcg aagaatatga acgtcgatat gatgaacatc aaggtttgct gttgggaagt
     2161 gtcacaacag cagttccact ttcagacgaa caacgaatac aattagaaaa gaatgttgca
     2221 aaaacaatga actatcaatc agtcgaatta aaacaaatcg ttgattcttc tattattggt
     2281 ggcgcgattg ttgaagctaa ccatcgagta atcgacggaa gcattcgcac gcaattagaa
     2341 aatatgcgta atcaattaaa tagatagtac aaagaggtag gtgaatcgaa tggccatcaa
     2401 agcagaagaa attagtgcct tgattaaaga acaaattgaa aattatcaaa acgtgctttc
     2461 agtcgaagaa atcggtaccg taacttatgt aggtgacggg atcgctcgtg cacatggttt
     2521 agaaaacgcg atgagtggtg agttgcttga attttcaaat ggctcatacg gaatggcgca
     2581 aaacttagaa tcaaatgatg taggtatcat tatccttggc gatttcgagt ccattcgtga
     2641 aggagataaa gtaaaacgaa caggaaagat catggaagtt ccagttggag aggccttgat
     2701 tggacgggta gttaatccgc taggtcagcc aattgacgga ctaggtgaaa tcgttactga
     2761 caaagctcgt ccagtagaag caatggcacc aggcgttatg caacgtaaat ctgttaacga
     2821 accaatgcaa acaggtctaa aagcaatcga cgctcttgta ccaatcggac gcggccaacg
     2881 tgaattagtt atcggtgacc gtaaaacagg gaaaacttcc attgcgatcg atacgatcat
     2941 caaccaaaaa ggtcaagata tgatctgtat ctatgtagca atcggacaaa aagattctac
     3001 agttcgtaca caagttgaaa cattgaaaaa atatggcgca atggattaca caatcgttgt
     3061 gaatgccggt gcgtctcaac cagcgccgtt gctttatatc gcaccatatg ctggtactgc
     3121 aatgggtgaa gagttcatgt acaacgggaa acatgtcttg atcatttttg atgatctttc
     3181 aaaacaagct gttgcttatc gtgaactttc cttgctgctt cgccgtccac caggtcgtga
     3241 agcttatcca ggggatgttt tctacttaca ctcacgctta ttggaacgtg cagcaaaatt
     3301 aagtgatgat ttaggtggcg gttcaatgac tgccttacca tttgttgaaa cacaagctgg
     3361 agatatctct gcttatatcc caacaaacgt tatttcgatc actgatggac aaatcttctt
     3421 agaaagcgac ttgttctacg caggtactcg tccagcggtt gatgccggac tatctgtatc
     3481 tcgtgtaggg ggttctgcgc aaatcaaagc aatgaaaaaa gttgctggga ctttacgtct
     3541 agacttagca agttaccgtg aattagaagc atttacacaa ttcggttctg acttggatgc
     3601 agcaacacaa gcaaaactaa atcgtggacg ccgtacagta gaaatcttga aacaaaaatt
     3661 gcatgcaccg ctagctgttg aaaaacaagt agtgattttg tatgctttga cacatggatt
     3721 cttagatagt atccctgtag acagtatttt agacttcgaa catgaattat tcgaatatct
     3781 ggataccaat cattcagata tctttgaaac aatccgtaca acaaaagatc ttccagaaga
     3841 agaagcgttg aacagtgcga ttcaggaata taaagacatg ttcttagcta caaaaggaaa
     3901 taattcctct acagaagata aattgaaatc cattcaaaat gcatagtgag gtgagatgaa
     3961 tgggtgcttc attaaatgaa atcaagacac gtatcgcttc aacgaaaaaa acgagccaaa
     4021 tcacgcgggc aatgcagatg gtgtctgctt ctaagttgac aaaatctgaa gcttcttcac
     4081 agaagtttca ggtttatgca acaaaagtaa gagaaatcgt tacgcatttg actgctactc
     4141 agttaaatga tattgcctca gaaaatccta gaggggatat caattacaac agcatgttgc
     4201 tcagccgtcc tgttaagaaa acgggttata tcgtcatcac tgcagatggc ggtttagtcg
     4261 gtggatataa cagctcgata ttgaaacaaa cgatgtctat tttagaagaa gaccaccaat
     4321 cacccgatga ttacgtgatg atcgcaattg gcggaactgg tgcagacttc tttaaagcac
     4381 gcggtatcaa tctagcatat gagctgcgca atctatctga tcagccaagc tttgatgaag
     4441 tccgcaaaat cgtaaatatg gcaacaacta tgtaccaaaa cgaagtattc gatgaattat
     4501 acgtatgtta taaccatcat atcaattcgc taactagtca gtttcgtgta gaaaaaatgc
     4561 tgccaatctc agacttagat ccagaagaag caactacttt cgaacaagaa tatatttttg
     4621 aaccttcaaa agaagaaatc ttgtctcagt tattgcctca gtatgctgaa agtttgatct
     4681 acggtgcgat cgttgacgcc aaaacagcag aacatgctgc tggtatgaca gcaatgaaaa
     4741 cagcaactga taatgctgct aatatcattg atgagctgac ggtttcttac aaccgtgcaa
     4801 gacaaggggc tattacccaa gaaattaccg aaattgttgc cggagcttcg gcactagaat
     4861 agtattgaga ttggaggaaa aaagatgagt tcaggcaaga ttgttcaagt aatcggtccc
     4921 gttgtcgacg tggaattttc tttagaccaa tccttaccag atatcaacaa cgcattagtt
     4981 gtttataaaa atgacgaaaa taaatcaaaa gttgttcttg aagcagcgtt agaattaggg
     5041 gacggagtga tccggaccat tgcgatggaa tcaacagatg gtttacaaag aggaatggaa
     5101 gtcattgata caggcaaagc aatctctgtt cctgtaggta aagaaacatt aggtcgcgta
     5161 ttcaacgtac taggagatac gatcgattta gaaacacctt tcccagaaga tgcggaaaga
     5221 agcgaaattc ataaaaaagc accagccttt gacgaattaa gtacaagtac agaaattttg
     5281 gaaacaggga tcaaagttat cgatttgctt gccccatatt taaaaggtgg gaaagtcgga
     5341 ctattcggtg gtgccggtgt tggtaaaacc gtactgatcc aagaactgat ccataatatc
     5401 gcccaagaac atggtggtat ttctgtattt accggtgtag gtgaacgtac tcgtgaaggt
     5461 aatgacttgt attatgaaat gaaagattca ggagttatcg aaaaaacagc catggtgttc
     5521 ggacaaatga acgaaccacc aggtgcacgt atgcgtgttg ctttgactgg tttgacgatt
     5581 gcggaatatt tccgtgatgt agaaggtcaa gatgtactgt tgtttatcga caacattttc
     5641 cgtttcactc aagctggatc tgaagtatca gccttgttag gacggatgcc ttctgcggtt
     5701 ggttatcaac caacattggc aacagaaatg ggtcaattgc aagaacgtat cacatctacg
     5761 aaaaaaggtt cgatcacatc gatccaagca atctatgtgc cagccgatga ctacactgac
     5821 ccggcgccgg caacagcatt cgcccatttg gatgcgacaa ccaacttgga acgtaaatta
     5881 acagaacaag ggatttatcc agcggtagat ccattggctt cttcttcaag tgctttagca
     5941 cctgaaatcg tgggagaaga acattataaa gtagcaacag aagtccaaca cgtattgcaa
     6001 cgatatcgtg aattacaaga tatcattgct atcttaggta tggatgaatt atccgatcaa
     6061 gaaaaagtgt tagtttctcg tgcccgcaga gtccaattct tcttgtcaca aaactttaac
     6121 gtggctgaac aatttactgg tcttccaggt tcatatgtcc ctgtagaaga aacagtgaaa
     6181 ggattccgcg aaatcttgga aggcaaatac gatgatcttc ctgaagaagc tttccgaagt
     6241 gttggccgaa tcgaagacgt cgtagaaaaa gcgaaaacac taggctacta gaaaggggtg
     6301 ccccatggat caatatttaa cagtaaatgt ggtaactcct gccggtttgg tctatgatca
     6361 ccatgcagct atcgtcgttg cacgtacgac agctggtgag cttgggattt tgcctaaaca
     6421 tgcaccaatc atcgttccat tgacgattga tgaagttcgt gtaaaacgaa cagattcaga
     6481 tacccacgtc gattggatcg ctgttaatgg cggaatcatg gaagttcgtg ataatgttgt
     6541 ttcaatcgtt gctgatagtg cggaacgtga acgtgatatc gatgtatctc gtgcggaacg
     6601 tgcaaaacaa cgcgcagaac gccaaatcga agaagcaaaa gcaaaacaag atacgaacga
     6661 attgaaacgg gcaactgttg cgttgcaccg agcaatcaat cgtatcaaag tatctaagca
     6721 ctcataaaat gagatgcgga tgtttatccg tgtttaagaa cagtaaaaag aggttatgac
     6781 agaagtggtc agcttcaaga cataagaagg aatttacgaa aattgcttct caaatttttg
     6841 tgaaatctca gcttattttt cgaagaagct acttctggaa caccgtatat cggaggttgt
     6901 gaaatcagcg ttttgtcctg agtattagag aaacaaatgg aaaatagttc ctcatatttt
     6961 gccgtttttt gagctaatac cgagggtcag atgattgaac accgtttatt tggaataagg
     7021 gactgcgaca agacttttgg gctctttgtc aataaggact gatgagttgt gcaaaatcaa
     7081 atctgagtca gaatgaacca cattctggc
//
LOCUS       DARDHA010000140         6886 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000140 DARDHA010000000
VERSION     DARDHA010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.142"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..182)
                     /locus_tag="SDA10_002280"
     CDS             complement(<1..182)
                     /locus_tag="SDA10_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG4241162.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="SDA10_002281"
     CDS             517..951
                     /locus_tag="SDA10_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG4241163.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="SDA10_002282"
     CDS             974..1294
                     /locus_tag="SDA10_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241164.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="SDA10_002283"
     CDS             1435..1626
                     /locus_tag="SDA10_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241165.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="SDA10_002284"
     CDS             complement(1697..1954)
                     /locus_tag="SDA10_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HEG4241166.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="SDA10_002285"
     CDS             2112..2375
                     /locus_tag="SDA10_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241167.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="SDA10_002286"
     CDS             2399..2587
                     /locus_tag="SDA10_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241168.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="SDA10_002287"
     CDS             complement(2670..2855)
                     /locus_tag="SDA10_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241169.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="SDA10_002288"
     CDS             3070..3441
                     /locus_tag="SDA10_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG4241170.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="SDA10_002289"
     tRNA            complement(3591..3663)
                     /locus_tag="SDA10_002289"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="SDA10_002290"
     CDS             complement(3709..4137)
                     /locus_tag="SDA10_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241171.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="SDA10_002291"
     CDS             complement(4197..4955)
                     /locus_tag="SDA10_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEG4241172.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="SDA10_002292"
     CDS             5250..5447
                     /locus_tag="SDA10_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241173.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="SDA10_002293"
     CDS             5553..6035
                     /locus_tag="SDA10_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241174.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="SDA10_002294"
     CDS             complement(6093..6689)
                     /locus_tag="SDA10_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG4241175.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         2331 a         1059 c         1353 g         2143 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc cccagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga attttt
//
LOCUS       DARDHA010000141         6627 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000141 DARDHA010000000
VERSION     DARDHA010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6627
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.143"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..443
                     /locus_tag="SDA10_002295"
     CDS             <1..443
                     /locus_tag="SDA10_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEG4241176.1"
                     /translation="YELEYEKSICSIPYNNIEEIIEKDAISIYKFFIFIPNRQLKANI
                     FNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDLS
                     MFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERILTI"
     gene            724..1944
                     /locus_tag="SDA10_002296"
     CDS             724..1944
                     /locus_tag="SDA10_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEG4241177.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1941..4661
                     /locus_tag="SDA10_002297"
     CDS             1941..4661
                     /locus_tag="SDA10_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4241178.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4645..5589
                     /locus_tag="SDA10_002298"
     CDS             4645..5589
                     /locus_tag="SDA10_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG4241179.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5658..>6627)
                     /locus_tag="SDA10_002299"
     CDS             complement(5658..>6627)
                     /locus_tag="SDA10_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG4241180.1"
                     /translation="AVLTLAACSGDTNKDIATMKGGTITVSDFYDEAKLESSNQSLVQ
                     RMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAGYTKDTYKEYIRNNL
                     AFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDVKKSADDGDDFSKLA
                     KDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDVITTTNPTSYATEYY
                     VVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEELKDANVKIKDDAFEN
                     VLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDSSK"
BASE COUNT         2335 a         1077 c         1273 g         1942 t
ORIGIN      
        1 actatgagct tgagtatgaa aaatctattt gttcaatccc ttataataat attgaggaaa
       61 ttatagaaaa ggatgctatc agcatttaca aattttttat ttttattcct aatcgtcaat
      121 tgaaagctaa tatatttaac catttgaaaa agatgctaaa tgtgtctgtt actgaatcgg
      181 cacctgtaaa tattgaaatc gtacctcaaa atgtatctaa aggtgtggtg tttgatcgtt
      241 tggaatcaat ctacaattta aagaaacctt tgagaatcgc gatcggagat agtttaaatg
      301 acttatctat gtttgaaagt gcagatatta gttttgcaat gggaaatagt catcaagaaa
      361 tcatgaaaaa agctgattat gtcactgttt caaatgataa agacggagta tctcaagcat
      421 ttgaaagaat tcttaccata taaactaatt aaggtagtat agaacataga attctaaata
      481 cggaaaaatg gagcataaat tccagtacaa atagtacttc ttcagtgtca aacgaagcga
      541 atataacata gatatttagg attgtgacaa aaatcatgtc acaatctttt tttcgaataa
      601 acttattttt tgaagtcggc catttatcgt aatttctttg cataatacga acaaattttc
      661 ggttattgat gaaaaaaaac attgaaaatg gtatgataaa agaaaaaaag gaggaaattt
      721 ttaatggtga aatttatcca tgcggcagat ttacatatgg atcggtcatt tgaaggactg
      781 acaactcttg ataaaactgt acaggaaaaa ctattgaaga cgaatctgac cgttttatca
      841 aatatcattg aacaggcaat aataaataac gttgattttg ttttacttgc aggagataat
      901 tttcatcaaa atcgtccttc attaaaaata cagaaacatt tttcagaaca gatgaaacgg
      961 ttagaaaaga accatatacc tgtatttata atattcggaa atcatgattt ctatcaaaaa
     1021 gagcggtatt ggttctcctt tcctaaaaat gtccatttat ttacatccga aacggtagag
     1081 acaaaaaaaa taacgataaa aagtggagaa actgtctctc tcagtggatt tagttatcgt
     1141 caaccgtgga ttcaaaaaga caaagtgatg gaatttcctt ctagagaact cacagattac
     1201 catattgggt tgtaccacgg ggaacctggc gtctctcaaa aaggaaacta tgctcctttc
     1261 caaccctcaa agatgcaaga aaagggatac gattattggg ctttagggca tatccatgtg
     1321 ccaacggtct taaatgaaaa gcaaacaatt gtttatccag gtgcgcctca aggtcatact
     1381 aaaaaggaac aagcttctac atctattcta ctagttgaat taagtaaagg aagttgccaa
     1441 atacatccga ttaaagtagc agaggtctat tggaagacaa aggaaatatc attgaggaaa
     1501 gcaagaacga caaaagaggt cattgttcac attcgtcagc agctttcaaa agtagaggat
     1561 ggatttagtt taattgagat aaaattaaaa gattatgacc atctcaatac agatgttttg
     1621 gaacggatac aatcaggaga gctattagac tatttgttag aggaattctc agataggata
     1681 aatgatttct ttatttggcg tatttctctc atagagaata ctttttatac gaagacccca
     1741 cttgctgctt ctgctaaatt gatggaacag ttatttcaat actatcagac acctcaagat
     1801 ttccaacaaa ttttaaatga agtatatagt catcaagaag cggctagaat attgagcgaa
     1861 ctgccggaat atcaagaaga aacacttgaa aaagccaagc agctgcttaa tcaagatttt
     1921 ttatttgagg aggatcagga atgaagattt taaggatcga gattgctgct tttggaaaat
     1981 ggcgtcaaaa aagttttgac ttttattcag gcaatcagct gatctatggg ggaaacgaag
     2041 caggaaaatc aacgatttat caatttattc aagcaatatt atttggtttc ccttcaaaag
     2101 gccgtaaaaa aaaggattat acaccaaaag acggctcagc ctatggcggc aaaatatggc
     2161 ttaaacatcc tgtatatggt gaatttgctg tcgagcgcta caagcagcaa aatcgcggaa
     2221 aatcgaaggt gtggctgggg gatcaagtgg gaagtgatga gttactagaa aaactgatcg
     2281 cacctttgac gaaagagtta ttccagcagg tatttacttt ccagcaagag cagttgatgg
     2341 aactagatca tttgagagaa aaagagctac atgatgcgct gatttcttta ggcatcacgg
     2401 gcagttctca agtattccaa aagcggcaag aatattatca gaaagcacaa caattattca
     2461 agaaaaaagg ccaaaaatta ctaatcaatc aaaaattaaa tgaatggaaa gagctgcaag
     2521 aaaaaattca tcaaaaacaa gaacaggaag cttattttgg caagttgatc caacaagaac
     2581 aggaatacga gtcgcaacag cagcagttat tggcacaatt aaaagaggcg aacaaacaat
     2641 tttcattagc cagacagcaa gaacttaatt tttctttata tgaagaatgg caatctctca
     2701 aaaaaaacag cactacaggt cttcctgatg agcaacttct tttagatctg gagtctttct
     2761 ccaaacggta tcagcaaaca aatgagcgtt tggaagaatt agaagcaaca ctggaaaagc
     2821 atagcgggat ggatcagcag tcagccagat attattttta tttagagcag gagcaggaac
     2881 taaaagatct ccagcaactt caatttacag cggaacaatt aaacgaagaa tggcaaagat
     2941 tacaaattga gactagagaa acaaattcag aattgaagat tttggaacaa aggtggcatt
     3001 ggcagaaaaa tccgttaccg gaagaatttt tatatgaaga agaatggaag catcttctgg
     3061 ctgaagaaag aaggattgct gatctagaaa gccagatttc tgtgcgtctc caaatagcta
     3121 aagaacagca agagatgatt gaagcagaag tgaatgactt ggaagagacc tatccagcat
     3181 tattacaaaa tcagtcagag atagcaccag tctcttcatc taaaccgtat atgctgatgg
     3241 ctggcggagc aataatcatt gcaggtatat ttgtaccaat gcccttgaaa gtgatccttt
     3301 tactaattgg tgcaggcatg attggctatt tcttctacaa aaggcaacca aaaattaaaa
     3361 ctaaaaatga tttctctgag ataaaaggaa tgtggcaaga aaaactagga caacttgatc
     3421 tagccttagc tcaattagct gaagcagaag aaaaacaaaa gagaactgga gaagaaaaac
     3481 agattcttca gcaagaagta tatcgttttg cggatcatcg gcacttagga aagatggata
     3541 cgctcaaact catgaaagaa tatggacagg aagtcataga ttatcagaaa ttatatggcg
     3601 tatttgagcg gaaacataaa agagaacagg aaatacaaga aaaactacga caaatcgacc
     3661 aaaaatttga gtttgttaaa gaatggttac cgatacaaga taagacaatt tcagaaaaaa
     3721 tggtattgct ttcccgtttt atacaagaga tggaacagat gaaatttgcc agaagctatc
     3781 agcaaaatac tttattgaag caagaaatca atcaattgaa aaagaaacaa aaagtatgga
     3841 tacaagaata cagcgaacaa ttgactcaag caggcttatc ttatccttcg gaagtacctg
     3901 tctttttaca aaaagccaaa caagccgaac agcagatgaa aagatacaat gaattatcgg
     3961 aaatgcttac tccactattt cctgaagagc tgacaaaaga gaaattatct gtaagactgc
     4021 gccaactgaa agaaaagcaa tcccaactcc agcaagaaga aaaagaagca agtgaaaatc
     4081 ggcaacgact ccaacttcaa gtagaagagt tgcaaagaga cggcacattg gacgaacttt
     4141 atcaacaagc tagccggcaa aaagcaaaat tacaggaatt actggaggaa tggggagcac
     4201 ttgaaatcgc cgggacattc ttaggtgatc tttcaacaga gatgagtgaa agacaattac
     4261 cccagttgtt aagagcagca ggtgattatt taggtatttt aacaaaagga aattatcaga
     4321 agatattact gaaaaatgat acactgactt taacagacgg atatacagaa tttcccatct
     4381 atgagttatc tacaggtacg aaagatcagc tgattatggc gatgagattt gcttacctct
     4441 cattagaagc taaccgttcg atatgcccga tcatcattga tgatggctgg cttcattatg
     4501 atcatcagcg aaagtatcaa ttagctgaac ttttagctaa atttggagaa aagtatcaaa
     4561 taatttgttt ttcttctgat aaagaaatgg taagctatta ccatgagatg aaccaaatcg
     4621 taaaacaatt agaaggagca aataatgaaa aaaattcgtg atttagtaat cgatgagcag
     4681 tttgaacaat ttgtcctgat caaaagtgca gatgtaagaa tcgctaaaaa cggaaaaaaa
     4741 tttattgctt ttactttcca agatacttca ggtactatcg atggtaaata ctgggatgct
     4801 tcggaggagg aaatcaataa atttacagcc ggcacagtcg tttttctaaa cggtaaaaga
     4861 gaggtttatc aaggaaaccc tcaagtaaag attcttcata tgcgcttaac aagggtagat
     4921 gagccaggag agcctagttt atacatggaa agagcccctt tgaagaaaga agaaatggaa
     4981 gaagaaatca atcaattgtt gtttgagatc accaatgcgc attggaatcg tattgttcgc
     5041 tatctactta ataaatatca aaaacaattt tttgagtttc cggcagcaaa aagaaaccat
     5101 catgcatttg ccagtggatt agcttatcat actttgacaa tgcttcactt agggaaatca
     5161 atcgccgatg aatatcctga gctgaatcga gcattgttgt atgctggtat cattttgcat
     5221 gatttaggaa aggtgattga actttccggt cctatgtcga cagaatatac gcttgcagga
     5281 aatttgatcg gtcatctggt attgattgac gaagaaataa ctaaagcctg tcttgcgttg
     5341 aaaatcagcg aagctgacga agatgttctt gtattacgtc atatggtttt agcacaccat
     5401 ggcttattag aatatggctc acctgtacgt ccaagaataa tggaagcaga aatattacat
     5461 caaatcgaca atatcgatgc ttcgatgcag atgatgttga cttcgattcg tcaaacagaa
     5521 ccaggaaaat acaccgatcg gattttcggt atggacaacc gaagtttcta tgtaccaaaa
     5581 gatttatagc aaaaatttat ttttttcatt caaaacaaaa aaagcgagac ataagtcttc
     5641 gctttttttg ttttctttta ttttgatgaa tcagttgttt cttcagttga agattctttt
     5701 gttgaatcag ttgattttgt gtcagaactc tttgttgaag ctgtagtttc acttgagtct
     5761 ttcgtacttg atgaagttgt tgtgaatgca ctcaacacat tttcaaaggc atcatctttg
     5821 atttttacat ttgcatcttt taattcttca ccgattactt tagttgtgaa tgcattgtca
     5881 cttagtttag tttcagtagc gatatctttt agttgatctt tgtatttatc catgtcattg
     5941 cctttatttt ggtttttcac cattttgaca acgtagtatt ctgtcgcata agaagtagga
     6001 tttgttgtag taataacatc ggagatctca ccgtctttca atttgaaagc tgcttctttt
     6061 acttctgcag gaatagttgt tgtcgttgag tcgaatttaa ctttaccgcc atcttctttt
     6121 gtctctgtat cagttgattt gtctttggct aatttagaga aatcatctcc atcatccgct
     6181 gattttttca catcttttgc ttcatcttca cttgataatt tgatgatttg tgcttctact
     6241 tctggatgga aagatttcca agcagttttc aaatcgtcat ctgtaatatc tacgtgagct
     6301 ttcaagccag cttcaaaagc taaattgtta cgaatatatt ctttataagt atctttcgta
     6361 tatccagcag cttctaattg tgattcaaaa gtatcaccta atgatttcgc ttgtttgtcg
     6421 tattctgcat ccacttgctt atcggttact ttatcgccat atttgttatt gaatacttta
     6481 taaatgatca tccgttgtac taaggattgg ttcgatgatt caagttttgc ttcatcatag
     6541 aaatcagata cagtgatcgt tccgcctttc atcgttgcga tatctttatt agtatcgcct
     6601 gaacaagctg ctagagttaa aacagcc
//
LOCUS       DARDHA010000142         6612 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000142 DARDHA010000000
VERSION     DARDHA010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.144"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(105..629)
                     /locus_tag="SDA10_002300"
     CDS             complement(105..629)
                     /locus_tag="SDA10_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4241181.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="SDA10_002301"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="SDA10_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEG4241182.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="SDA10_002302"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="SDA10_002302"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEG4241183.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="SDA10_002303"
     CDS             complement(2351..3031)
                     /locus_tag="SDA10_002303"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEG4241184.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="SDA10_002304"
     CDS             3157..4026
                     /locus_tag="SDA10_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG4241185.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="SDA10_002305"
     CDS             4052..4567
                     /locus_tag="SDA10_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241186.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="SDA10_002306"
     CDS             complement(4614..5207)
                     /locus_tag="SDA10_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4241187.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="SDA10_002307"
     CDS             complement(5232..5411)
                     /locus_tag="SDA10_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241188.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="SDA10_002308"
     CDS             complement(5488..5997)
                     /locus_tag="SDA10_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241189.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="SDA10_002309"
     CDS             complement(6093..6473)
                     /locus_tag="SDA10_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG4241190.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
BASE COUNT         1859 a         1395 c         1171 g         2187 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aa
//
LOCUS       DARDHA010000143         6570 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000143 DARDHA010000000
VERSION     DARDHA010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.145"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            127..579
                     /locus_tag="SDA10_002310"
     CDS             127..579
                     /locus_tag="SDA10_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241191.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="SDA10_002311"
     CDS             593..1282
                     /locus_tag="SDA10_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241192.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="SDA10_002312"
     CDS             1407..3758
                     /locus_tag="SDA10_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEG4241193.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="SDA10_002313"
     CDS             3838..4233
                     /locus_tag="SDA10_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241194.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="SDA10_002314"
     CDS             4558..5175
                     /locus_tag="SDA10_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG4241195.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="SDA10_002315"
     CDS             5216..5515
                     /locus_tag="SDA10_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEG4241196.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="SDA10_002316"
     CDS             5549..5716
                     /locus_tag="SDA10_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG4241197.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="SDA10_002317"
     CDS             complement(5761..6366)
                     /locus_tag="SDA10_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG4241198.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2265 a          910 c         1285 g         2110 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaattttt
//
LOCUS       DARDHA010000144         6567 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000144 DARDHA010000000
VERSION     DARDHA010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.146"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(7..1164)
                     /locus_tag="SDA10_002318"
     CDS             complement(7..1164)
                     /locus_tag="SDA10_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4241199.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1167..1523)
                     /locus_tag="SDA10_002319"
     CDS             complement(1167..1523)
                     /locus_tag="SDA10_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241200.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="SDA10_002320"
     CDS             complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="SDA10_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HEG4241201.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(2991..3830)
                     /locus_tag="SDA10_002321"
     CDS             complement(2991..3830)
                     /locus_tag="SDA10_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG4241202.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            4015..4902
                     /locus_tag="SDA10_002322"
     CDS             4015..4902
                     /locus_tag="SDA10_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEG4241203.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(5486..6181)
                     /locus_tag="SDA10_002323"
     CDS             complement(5486..6181)
                     /locus_tag="SDA10_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEG4241204.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(6165..6563)
                     /locus_tag="SDA10_002324"
     CDS             complement(6165..6563)
                     /locus_tag="SDA10_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEG4241205.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         2065 a         1301 c          966 g         2235 t
ORIGIN      
        1 cttttcttaa ttaacgaaat ccaatttttc caatatggat ctatttttct cattcctgat
       61 ttttatgatt tcagctttca gacgcgccca atcatttttt gtaggttgtc cattccagta
      121 aaatatctgt gtattcgttt gatcatatgc ctttttgtcg ataggataat cgctaatgac
      181 aatatcaggt aactgatcag cggatgcact atattttatt gggcgttcag tcatagataa
      241 tatccttttc tttgcccata attcctgtgt tttaccatac tttgattgaa tatagaatat
      301 gacaggttgt tcgtgtttta taaaaatatc cctgactaac atacagtatt gaaaaatcaa
      361 ccgtctgttc ttttcataat aagtattaag ctctggaatt ctgcgagcta ccccactaaa
      421 aaactgttgc atgattggat acatcactgg aaatatatgc tgaaaatctc tgtcttctgc
      481 tgattttcca aaagcatcca ccttcttttt tgtcccaaaa acccttgaat acaaatggat
      541 cattgccaaa ttgacatata gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt
      601 tttttcaaaa ttttctacaa tagacgattc aatccagccc caactttctt gtactttatc
      661 tggagtatcc aacaaatctt cttccaaata ggtgttcatt acaccaaaca taaaataaag
      721 gtaagcttgc tctgttggag tgatgtgatt catcgaccag aagtttgctt caaaaaaagg
      781 ctctagtatg taatgcgtaa actcttcttt tgttatcaat ggatgcacaa atttgttcat
      841 ctcttctggg acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag
      901 taggctgatc catttctttc tatctatact acgcgtatat ggcgcatgtt tcgcaaattt
      961 ttctaatagt tgacgtttat tttttatact tgataatata tcagaatgat tttcgtcgaa
     1021 tgactgccaa tacatcaagt gaaaaaagta tcggatacgg tattcttctc cttctatcgg
     1081 attacctttt ttcaagctgc aagataaatg atagtttttc aaaatagtat taagttctct
     1141 taattttctt tgaatcgtac gcaaacttat ataatttttt gactaagatt ttctaaggat
     1201 tgataagtac ctttgtaaag tgaatccagt aaaatataac caatcgttcc ctctaaaagt
     1261 atagaataaa ctttactcag taaaaactga gaagaaaact cgacttttat catcttgttt
     1321 tctgaaagaa aagataattt atattctgga tacctttcgc aaaacgattc tgcttgttga
     1381 atcaccgaat tcatcttagc cagggtgtag tttagatcta aagcgatatc cttcaccaca
     1441 tataatttct cgtctctttc ataacaatag ttcaaaaatt gatatacttg cctttcattg
     1501 atatcaataa attcttcaaa caaagtacat actccttttt atctattatt tagacttatt
     1561 ccgacagcta catgaataac gtacaaatag attcgttact gatttttcag agatactacc
     1621 atgcgcagtg tgcattgtca attattcttg ctcttatttt ccatttgtgt tcctgttatt
     1681 tcctgtttct caaatatgtt agtaaacaaa aatagatctg gcaaagctac aacttcgcca
     1741 ggcaatacag cactctcgaa tgaaatattc actcaaaaat ctatcgtttt attcaatttt
     1801 agatctgttg cttttattct ttatttcact cgatggaaga gagaacgaaa atagcttgct
     1861 cctacaatca aaatgagtaa cacacccatc catgtaatta gcttgttcaa ggttctacta
     1921 ccagtttctg gtaaatcgct ttttctatta tctgtttctt tcgctttttc ttgatgatca
     1981 attccacgag aaacaaaatc aggctgtttt agcacacttt ttaggttgct ccctgcattt
     2041 ctcttttgtt ccttagttgg tatgataggt accaaatcaa gttcattatt tacttttcct
     2101 gtgctgcttt cgctgctcgt gcttcctgtg ctactctcac tactcatgct tcctgtgcta
     2161 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgcttcctgt gctactttca
     2221 ctactcgtac tctccgtact gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc
     2281 gtactctctg tactgctttc accgctcgtg ttttctgtac tgctttcact acttgtgcta
     2341 tccgtactgc ttggcggtcc aacaactgtc caactgcttg gtttcaaatt ctccgtgatc
     2401 gtcatctgtc catcataaaa aacaatcaaa tcaactttat ctttactctt gatctcatca
     2461 atcgatagtt ctgcagaaat cattattgta tactcaatac cagcggtcca ttgtacgtct
     2521 tttaaataaa cttccccaat aactttttct gaacttccat taccatccca accatatgtt
     2581 agaacagcat gtacatcact agcattcacg cttttgtttg gtttgactgg aatagtaatg
     2641 acttgttgtg tcatgtcata aacaatccga tcagttttca cagtaaatga aagattctca
     2701 tttgcccgtt catttgcaga agtactgatt ccaatcattg ctgcaattat actcagccca
     2761 agaataacaa ataatacggt cgctgttttc ttcatttcta tcactctcca tataattacc
     2821 ttctatttga taaaggaatt atataaaatg aaaatgatga gaacacgaca gtcagttgtg
     2881 aaaatctaac aaaagatgtc gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa
     2941 tgacagactt aaaaaagcaa gaccgatatc agccttgctt tcttcatctt ttattgataa
     3001 acagcagtgt atttcttcat ccgctttgtt ttatatgcat agagtaacaa accaacaatc
     3061 aagactaaca agaataatgt agttgatata gatgcttcag ccccaaagtt tcccccagta
     3121 attattgtat tttgcgattg gatcagagtg gagaaaaaac cttgttcttg cgttccgctg
     3181 actggtatac ccaaaatagc tccttgtgtc caattccaag taatatgagc accaatagcc
     3241 gtaaataaac ttcctgtact gtagataagt gcgctgaaaa agcaacctgc taaaaagata
     3301 ttgaaaactg ccagaaatgt catgttagga ttcaatccgt gcaaaagagc aaataatgca
     3361 gcagatccaa tcattgctat taacggacca aatctaagca tctgatgctg aaaaaagcca
     3421 cggaataaaa gctcttctga tgcccccact gcaataaata aaataaaact cgttatcagc
     3481 ggcacaatgt ttgcgtttcc tgaatctaac cgatacgccc ccgtcaaata taatggacta
     3541 gtaataagca gaaacactcc aattccaaca acgagacctc ccgctaaata cccccagtct
     3601 tttcctcgaa cagaaacacc ataatcttct attctttttc cttcgacctt actagcaaga
     3661 aataaagcta gtaaaaaaac agctaaatgt ccgaccgtac cggcaattgc tccaatccat
     3721 tcactatttc caataaacat atccgcaagt gcattattga caagaattcc taaaacaaaa
     3781 ccaactaaga ctattaaaac agatccaatc aaccgaataa tcctgatcat ctttcatccc
     3841 tcctttatat taagtttaac aacccttttc tttcttgtct cgtgaaaact atgtaaatgt
     3901 tctccatcat agagagaata gatttttacg aactaagaat atctacagca accttacgaa
     3961 aatgaaagac tgtctcccta attcttctgt tagactgttt ttaataagga aaggatggga
     4021 aattttttga aaaaacaact actaattatg tgcctttcag catgtgcaat tttactattc
     4081 tcaggttgta gcaagtccaa tgaaacgatt gcaacgttta aaggtggaac acttacagtt
     4141 caggattttt atgataaccc aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg
     4201 attgtcttca aagcatttga tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca
     4261 aaagaatata atgaacaaat caaaaaactc ggtccaaact acaaggaaca attaaaagca
     4321 gttggccaaa cagaagagac atataagaaa ctctttaaac agatgctggc tttccaatac
     4381 ggtttgaaag ccaatgtaaa attaacagat aaagatctag acactgcttg gaaagaattc
     4441 tatccagaag ttagtacaca gatcattcta ttttcgacta aagaagaagc agataaagca
     4501 aaaaaagaag caaatgaagg ggaaaatttc tccaagcttg ttcaagctta tggaaaaaat
     4561 aaaacattaa aggaaactga tggaaaaatg aattttgatt caaccaatcc agagattcct
     4621 actgaagtaa aaaaagcagc atttaaacta aaaaacgggg aagtttctga tatcattcct
     4681 gtaacagatc caacgactta tcagcaaagc tattatctag taaaaatggt aaaaaaacag
     4741 gataaaggtt ctaataaaga caaatacaaa tctgagctag aaaaaattgc taccgaagca
     4801 aaaactcttg atacggaatt catggataaa acaatccgta aagtgatgaa aaaagacaat
     4861 gtaactatta aagatcctta tgtaaaaaat atattcaaat aaacaaattt gacccaattg
     4921 gggatgtggc aaaatttgag aagtgatttt tggaaattcc ttcttatttt tgaggcaaac
     4981 cacttttgcc acaacttcat cggggccttt ttttatttgt accaaaacaa atcactggat
     5041 ttgttacaat catgaggata taatttaaaa aagtttttta tttctatcac atgaatcaat
     5101 gcgccattaa gtagattctt tcatgcttcc ctattttttc acttgccgta tcgcttcaaa
     5161 aacaactatc ataaactcaa actcgactga aaagttattt taaaaaagac taaaaatatc
     5221 ccttctggac ttgttcctct tattctgtta tactagagaa acacgaaaga tatttttatg
     5281 cgacactttt catttgtttt tatgaaattt attcaaaact ggcgtaactt cagagttaca
     5341 aggatgtatt agaggttagg gaatacatcg tttaaatgag aaataagtaa tagtcaccca
     5401 aactttaaat taatgaatat tgaatcgtca gatagcctcc tcgagttttc gagaaggcta
     5461 tcgtttattt tatggagaaa ttgattcaga agtaatcagc gacctttttt agttcaccaa
     5521 tcgtttctgc ttggaaagtt tgcacccaat cttcttggaa gcccctcact gctttatcaa
     5581 tcgagggcat ctctaatact tgatctagta gctctttacc taacgttatc gcatgagctc
     5641 cagatcgaat ggctgtagtg acttgttcac aatttttgaa actagctgct aatatttttg
     5701 ttggtctttg cgtacgttcg atctcagtcc gcaacgaatc aatcactttc atagcatcaa
     5761 tgccttgatt ttgcatcctg ttataataag gtgcaatata atctgctcca ttattaatag
     5821 ctaaataccc ttgtaattct gtgtaaatag ctgtaccggt aatcaaataa ccttcttttt
     5881 tcaacttttt gatcgcttga aggcctgctt gattcaccgg caccttgata aacgtatctt
     5941 ttcccagttc ttctatgata actcgagcat cctctatcat ttcttcttcc gttcttccta
     6001 ttgtctgaat atggatagga atctgtccga ttattttttt gatttctttc atatgcgaga
     6061 agaagtttga cgctccttct tttttcagaa ttgtaggatt tgttgttacg cctgttaaat
     6121 tcaaaaactg ttgacaagaa taaatacttt ttgtatttgc tgtatcaagc atgatttcca
     6181 tatagctttc ccccattctg atcctcttga tcgattttaa tgattgtccc ctttcttaaa
     6241 tgcggcattt ccttatcttc tacatacatt gttcctggaa gttccggctc atgagatcca
     6301 tcaaatttta tcgtgatatg tcctaattca ttcaaattag tttggacaac atctcctact
     6361 gctgtgatta catacatttg tttatcaaaa cagagcttca tatcacaagt tatttctttt
     6421 cttacctgat tcaattcaat cttataacaa taatctgtta atgtttccgg tgcttcatct
     6481 ccaaataaaa tcattatttt ttctttcctg aatacttctg cttctatacc aacatctgtt
     6541 acaaccgttt caaagatcgt catctct
//
LOCUS       DARDHA010000145         6415 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000145 DARDHA010000000
VERSION     DARDHA010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.147"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..2606
                     /locus_tag="SDA10_002325"
     CDS             <1..2606
                     /locus_tag="SDA10_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HEG4241206.1"
                     /translation="LRTFSTLNKKTVSHIFSTADSQAIINYLEKQKLSEGTVNITYNP
                     IKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKEGSATDSEKKKAENVMEQVERYLKE
                     KRIESFVKVDSGNGYHIFLPINEQPNNHETILTIQNFLQLLHRRFGVEDEVDIDTSVY
                     NPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPDNIDRSNCFKILEQIVEENSMMSTS
                     RVKSHPSFREVLPFIQADYKQWLAHYPELTYTIKEGDHKGVTLLIFDECPLRKHTNNS
                     NGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKYPLPKPVNPSVESLESNQEYYFDQF
                     KLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQKVITLVLAYYVDHCWDEIEIEGGCL
                     QTNNFKQLAKHGLIIKARREAEVVDFLQIQRENAPIFFEHESLGWKKENEAMIFQLQD
                     SFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKYVLGTNLELAWSIGIASIVLGYLNV
                     TKANTFLSLLINLQGKSTTGKTTTLHFIASLFGNPTTVLRNMNATNKAIIKLASNNQG
                     GVPLILDELGAATQNNFSSLIYQLASGEERLRLDSNCQLMPQETFSVLTLTSSEERLE
                     NYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKSLSSNTFGWGIKLFLLKLFEQGITK
                     IEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYSGALLIQDILGYPLRLEHIQTVLIN
                     SYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLTNSDNKHTPSTIWGKVSIQKNGDIK
                     VNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNMCAEKGRRTKRIHMNRQVLPTYEIL
                     LPSSLKSYFRL"
     gene            2788..3027
                     /locus_tag="SDA10_002326"
     CDS             2788..3027
                     /locus_tag="SDA10_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241207.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3043..4416
                     /locus_tag="SDA10_002327"
     CDS             3043..4416
                     /locus_tag="SDA10_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG4241208.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4420..6054
                     /locus_tag="SDA10_002328"
     CDS             4420..6054
                     /locus_tag="SDA10_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG4241209.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6217..>6415
                     /locus_tag="SDA10_002329"
     CDS             6217..>6415
                     /locus_tag="SDA10_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241210.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2247 a         1049 c         1291 g         1828 t
ORIGIN      
        1 atttgagaac tttttctaca ttaaacaaga aaaccgtttc tcatattttt tctactgcag
       61 attctcaagc aattattaac tatttagaaa aacagaaatt gtctgaaggt acagtaaaca
      121 taacttataa tcctattaaa caagaggtga ttacacaaaa aaagcattct gtgagagaca
      181 atgatattga aaaaattcgg tttgtattta ttgatattga ccctaaaagg aaagagggaa
      241 gtgcaacaga tagcgaaaag aaaaaggcag aaaatgttat ggaacaagta gaacgttatt
      301 tgaaagaaaa gagaatagaa tcatttgtaa aagttgatag tgggaatggc taccatattt
      361 ttcttcccat aaatgaacaa ccaaataatc acgagacgat tcttactatt cagaattttc
      421 ttcaattact tcaccgaaga tttggagtag aagatgaagt tgatattgat acttctgttt
      481 ataatccttc aagattatgc aaattagttg gtacaccagc ggtcaaagga aagtccacaa
      541 aagagcgtcc gcatcgttta tctagtttcc tttctattcc cgataacata gatagaagta
      601 attgttttaa aattcttgaa cagatagttg aagaaaattc catgatgtca acctctagag
      661 taaaaagcca tccatctttt agagaagtgt taccttttat tcaagcagac tataaacaat
      721 ggttagctca ctatcctgaa ttaacttata ccataaaaga gggcgatcat aaaggagtta
      781 ccttacttat atttgacgaa tgtccattaa gaaaacacac gaataattct aatggttctt
      841 gtttgtctgt tacggctgat aataagattc gatttcattg tctgcatgct tcggataaaa
      901 ataaagatat tcaagacttt gtaaaaaaat atccgttgcc aaaacctgta aatccttctg
      961 tagaatcatt agaatccaat caagaatatt attttgacca gtttaaactg aatcaagaag
     1021 gagtattttt ggtgaaagaa ggtcaagaag aaaaagttgc ttgtccgatg tttattaagc
     1081 aaatacgcaa aaatatcgaa caaaaagtca ttacacttgt tcttgcctat tatgtcgatc
     1141 attgttggga tgaaatagaa atagaaggtg gatgtttaca aactaataat tttaaacaat
     1201 tagcaaaaca tggcttaatc attaaagcta gaagagaagc tgaagtcgtt gactttttac
     1261 agattcaacg ggagaatgct ccaatctttt ttgaacatga atctcttggc tggaaaaaag
     1321 aaaatgaagc aatgattttt cagttacaag attcttttgg acgtgaaatt ccttctgtat
     1381 taactaaaaa ctcatgctac caattaacaa ctaatggtac agaagcggaa tttgacgcat
     1441 tggttcataa gtatgtttta ggaacgaact tagaacttgc ttggtcgatt ggtattgctt
     1501 ctattgtgtt aggctattta aacgttacga aggctaatac ttttttgagt ttactaatta
     1561 acctacaggg caagagcaca acgggtaaaa ccactacgtt acactttatt gcctctttat
     1621 ttggaaatcc aactacggtt cttagaaata tgaatgctac caataaagcc attataaagc
     1681 tagcatcaaa caatcaggga ggtgttccac taatactaga tgagttagga gcagcaactc
     1741 aaaataactt tagctcgttg atctatcaat tagcttcagg agaagaaagg ctaagattag
     1801 attctaattg tcagcttatg ccacaagaga ctttttctgt tttgaccctt acctcttctg
     1861 aagagcgttt ggaaaattat ttcaatgaga aattaactgg tttgaaagtt cgatatatcg
     1921 aattttctga tatcaagttt acaaaggatg cgaatcacgc agagaaagtt aaaagtcttt
     1981 cttctaatac ttttggttgg ggtataaagt tatttctttt gaaactcttt gaacaaggga
     2041 ttacaaaaat tgaagcaact tttgtcgaag ctcaaaaaaa actatgggct gttttacctg
     2101 aacattcttt gaagcaacgc attggtaata atctagcttt gatttatagt ggagccctcc
     2161 taattcaaga tatactgggc tatcctttac gattagagca tatacagacg gtgcttatta
     2221 attcgtacga acagttaatt gtagatacta gtttaggaag tgagcaaacg gtatttgacc
     2281 gagtgaaaac attgttggtg atgaatagcc ataaattttt aacaaacagt gacaacaaac
     2341 atacgcccag tacgatttgg ggaaaagtat caattcaaaa aaatggggat attaaggtaa
     2401 atatttttcc tatcccgttt gaggacttgt taaggcgaga atttcaagta aaggatctaa
     2461 cctatctttt taggcaattg cttgattcgg gtaatatgtg tgcagaaaaa gggagaagaa
     2521 cgaaacgtat tcacatgaat cgtcaagttc ttccaacata cgagattcta ttaccttctt
     2581 cactcaaatc gtattttcga ttgtaattta gaataaacca aagtatgaaa gtgaggcacc
     2641 atgttatgga aatatttctt attgatttag caaaacaact gattacttat ctgtatgata
     2701 gcgagcaaac ccaaacggaa gtaaatgaag cttgatctat gcttcattta cttcattcaa
     2761 tcaaacatga aagaaaggaa aattctaatg actaaaaata aaatgacact aaaagcagag
     2821 gttcttctgt atattcaaga acatttttca aatcaagcct tttttacaaa accaatttac
     2881 ttggatttcg aaataagagg ggtatctgct ggatctatcg ggggtactct tcaagcgttg
     2941 aagaacgaag gatatttaga aaatcacttt gtgcaacgtt cgttcaatag aagagtcgtg
     3001 aaaaaatggt atttagttca ttcatagaga ggggaatcaa gcatgcgtgt ggcttgttat
     3061 gatcgaacgt ctgtttatga agaagcgaaa attagtaatg acgctcaata tgatgcagtg
     3121 aaagagtatg ttttagagaa atatgaacaa attccccaac gattttcaga caaaggagaa
     3181 tcaggggaaa aagcggagcg tccagctttt cgacagctta gagcttgggt ttgtgcgtca
     3241 gaagaagagg gggtgatcta tgtttggcgt tatgatcgat tggcacgtca agccaaaata
     3301 gccttagaat ttattgagtt gtgccaacaa catcaggttc aagtgtattc gatttctgaa
     3361 cctcttcctg cttctttctc acacgcgtca acgaataaac tatttgtgca attattgttc
     3421 acttttgcag aacaacaacg aaacgtcatt attgagaatg ttcgtgccgg actttcctat
     3481 aaaaagcaac aacggcaata tttgtcttct gcggtaccat atgggtatcg tttagtgaat
     3541 gggaaaatcc aacaagaagt acacgaagca aggattgttc gctatatttt tcaactttat
     3601 ttgaccaaaa aatatggcta caaaaagctt tgtcaacgat tgacgcaaca aaagcttttc
     3661 ttccgcgaac gtccttttca gccctatcat atttatagta tcctaaaaaa tccattgtac
     3721 tatggcgaaa tcaaaggagg atctttgggg aagtatctag gaacctttga accgattctc
     3781 tcgaaagcaa cctttttaca agcccaagag attcgccaat caaggtgtac agctaaaaaa
     3841 gatacgtatc tgtacttatt acgtcaaaaa attcggtgtc ccttctgtgg tcgtcatctg
     3901 agttcgaagt atcaatggaa tacaaagaag acgaagacgc ttcattatta tcattgtacc
     3961 gaccgaagct gccaaggaat ttttcttcgg gcagaaaggg ttgagtcaga agtattggaa
     4021 aagatacagg ctttttttca atacgaaggc gtctatcaag ccttgattgc ccaattaacg
     4081 caacaattag ccgaaaagcg aaaagatgaa aaacgcaaag taatccaaca aaagaaacaa
     4141 gaagcacaat ggattaaggc atttgaagag ggcaattgtt cactggaaga actcaagcaa
     4201 caactgcaag ctcttcaaaa gccgatgtct acttcttcgt ttctttcact ccaacagtgt
     4261 gaagcaaaag tgaaagaatg gcttcaacta aaagacctct ctagtcgaca attattatgt
     4321 gactatgttt gtcggattga ggtaacaaag gataaaacag tttgtgggat ttacttccga
     4381 caactgacac aaaatatttt agaaaggaac gaatgatcta tggaaatgat acaaaccaaa
     4441 cgagtcgcat tgtatattcg cgtgagtaca caagaacaag ctttagaagg acatagtatt
     4501 caagcacaag aacaaaatgg acgacagttt gccgaacgga tgggctatga agtggtagaa
     4561 gtttatgccg atgaaggaat cagtgggaaa tccacaaaaa atcggttcgc ctatcaacga
     4621 atgatgaacg atgctcggaa ccatcgtttt gatctactag tgatttggaa attgacccgt
     4681 ctcggacgca atatgttaga tgtcttacaa acagtcgagg aattattgcg atatgacgta
     4741 ggattacatt cgatctcgga acaatttgac gtgaccactt cgacaggtaa attgatgtta
     4801 caactccttg ggagtttcgg tgaatttgaa cgcaaccaaa ttgcggagaa tgttcaaatg
     4861 tcaatgcaaa gcttagtgaa agataaaaag cgttatgcag gaggccgacg tttaggctat
     4921 gtcagtggta ttgacacgaa tggtcaaaaa caattggtca ttgaaccaga agaggcgaaa
     4981 attgttcgtc tgatctatgc aaaatattta ggcggagcag ggtatcgagc gattgccaat
     5041 tttttgaatc gtcaaggata tcaaacagtc aaaaagaatg ctttttctac cacggcagtc
     5101 aaagatatct tacgcaataa aatttatgca gggtatttag aatatgcgcg ctacatcaat
     5161 tgggaaacca aacgacggaa ggggaagaat ccgagaccga ttttagtgaa aggaacccat
     5221 gaaccgatta tttcggaaga agattatcaa aaagtacaag aacgccttgc tttagaagga
     5281 aaacaaccca aatggaacca tacaggtgaa aatgtattga caggcttgtt acgttgtcca
     5341 gaatgtggcg caccgatggc agcgagtaat gtcacaaata ccctaaagga tggcacaaag
     5401 aaacggattc gatattattc ttgttcagtc tttcggaata aaggagcaag tgtgtgtcat
     5461 gccaattcga ttcgtgccga acaagcggaa acatttgtcg ccgaccgatt aaaggagatc
     5521 gttcagctac cagaagttct tccccgattg gtggcagcgt taaatgaaga aatagtgcga
     5581 caaagccaac cattggaaca agaactcgtg gtgttactgg aacgaaagga agaattgaag
     5641 actaaaattg aaaaatggga agctgctttg gaagatagtc ctgaattgtt tccgatgtta
     5701 aaagaccgct tagacgaatt gactgaaaaa cgacgacagt tgcacattcg agaaaatgaa
     5761 atcttaggga tctttcaaca aggagagccg attcaagtca aagatgtcca acggatattg
     5821 actaatttgg atcgttttct tgcccagagt gagaagaagc aaatcaaagc cttgtatcgt
     5881 acctttatag agaaaataac ctttgatcct aaccataagg agatgttgga aattacgatg
     5941 aaattcacgc atgccgtggt acaacaattg aatgagcaat atcaaatagc cgtatccaaa
     6001 acaaaggata cggctatttt tgtgttaaaa acacccttta cgttaacgat ctagtctagg
     6061 ttatatcaaa caacgttaga ggggcgctaa cgcttctcta gcgatttgaa atcgttagag
     6121 aagtagttag ggaggtcctt atcataaata gggatattta ttaatgaata tttgaaatat
     6181 aattgtatat aagaattaaa aagagtgggg atagatatga aaaagatggg taagtgtatt
     6241 ttatgtgaaa aatacactga acttactaaa gaacatgtac cacctaaaaa atcagggaat
     6301 actggaggta aaaagaccag aactggtaat ttagatgatt tccttaagag cgattttaca
     6361 aaaggtgatt ttccgaaggg gattaaaagg aaacctcaag gaaatgttta ttata
//
LOCUS       DARDHA010000146         6402 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000146 DARDHA010000000
VERSION     DARDHA010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.148"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(93..1055)
                     /locus_tag="SDA10_002330"
     CDS             complement(93..1055)
                     /locus_tag="SDA10_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4241211.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1057..2496)
                     /locus_tag="SDA10_002331"
     CDS             complement(1057..2496)
                     /locus_tag="SDA10_002331"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEG4241212.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2681..4627
                     /locus_tag="SDA10_002332"
     CDS             2681..4627
                     /locus_tag="SDA10_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEG4241213.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4891..5763
                     /locus_tag="SDA10_002333"
     CDS             4891..5763
                     /locus_tag="SDA10_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG4241214.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            6059..>6402
                     /locus_tag="SDA10_002334"
     CDS             6059..>6402
                     /locus_tag="SDA10_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG4241215.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALECFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTG"
BASE COUNT         2041 a         1115 c         1139 g         2107 t
ORIGIN      
        1 gttgacaact cctttttgtt tacaaatcaa ttggatatct acaaattgta ttttctaacg
       61 aattttatag acattaaaaa attaatttaa cgttaaatac ttcctccaga aagtattgag
      121 attggtaaaa tataatcctt atttgtcttt tcgcctttaa tttttttcag aagaatatca
      181 acaataagac ttgcaatttc atcaattggt tgtcttattg ttgtcaattg tgggaaaaat
      241 ttctcaataa aagaggtacc atcgtatcca ataattttta aatcttctgg gatattaagt
      301 ttaagttgat gagcaatttt catagtgaga atggctgtta aatcatcaga aacaaaaata
      361 ccatctggtt tgtgatttat aatagttgac tttatttcca tttcacgacg gatagtagac
      421 agattattag gaattttgaa aatttttcca tttggatttt gaaaagaaaa acctagtgca
      481 cgtaattctg tgggagaatc cgtgttatca tttcctgtaa tcataatggt attttgacaa
      541 ccattttttt gtaaagtctt agctgcaagt tttccccctt caaagttatc tgaagaaaca
      601 atggaaacat taggagctaa atttctatca aaagcaacaa taggtgctct cacacgctca
      661 taatcagcaa ttccaagatt atggcttgat gagattatgc catcaacttg gttggcttca
      721 agcatttcaa tgtattctct ctctttagca ggatcatttt gactattgca aataatcgtt
      781 ttatatccat gtttaaataa ctctatttcg agatactcaa tgagttcaga gtaaaagata
      841 ttactaatat taggaaaaat aagaccaata agctgaggtg actttccttg aagtccacgt
      901 gctaaattat ttggtttata accaagagtt ttcatagctt catgaacttt ttgttttgtt
      961 ttttctgata aatatccctt attattaatg acgcgtgaaa cggttgttgg gctaactcca
     1021 gctaattcag cgacatcagt taattttgct accattttaa tattttaatt caaagtattt
     1081 tccagtaggt tcgcctgatt tgatttgaat accattttgg tgattttcag ggaatacacg
     1141 actagtaaac actttttctc ctttattaat aaaaatctca atgattgagt tatctacaaa
     1201 aatgttagca gttgtttctt tagtatcaat tgagcattca cgaatcgtac caaagtcagt
     1261 ggcatattga atgcctgctt tgctgcgatc taaaataatt tttccttctt tggtatctac
     1321 aatcaagcta agtccatttc ctttactatc agagaaaaga agtaactccg atttctgatt
     1381 tggaggaaaa gttagttcaa gctcataagt attatttgtt tctgcttttg ctgtaaattg
     1441 ttcagaacta gcacgtaatg atgtaatagc ttctactgga tattggtaaa gtttaccatc
     1501 ttttattgaa agttctttta caagactcat tgctccctga taatcatact tatcagttgg
     1561 ataatcgata tcaggtagtc caatccaact gacaatcaga gcacgaccat caggagtatt
     1621 gaatccttga gtcgcatacg cttcaaatcc ataatcaaga ttttgaattt ctgaagcgcc
     1681 cacaagtttt actttttcta tgtcaagcga ttgacagatc ttaaaagtat tcggatagat
     1741 attcccataa tttagctcag atttatccaa cccttgagga caataaatga gaacaggttt
     1801 tttgtctaca aatactagat ttggacactc aatcatgtat tctgaaccag tgccgccaaa
     1861 atcaaggttg ccaacttcct cccatgtttc cacgttattg tcatccgctt tgtaaagttt
     1921 gataaagcct gatttttcta aattttgtgc tccaacaatc gcatacaatt gtccttcata
     1981 attaaacatt tgtggatcac gaaaatgatc agttacatca gcaggtttat gaataagaac
     2041 attctcacct ttcgtaatgt tgtagtcttt atccatccat gctccgattt gaagtgtatc
     2101 acgttcccaa ttttcatttc gaacgtttcc tgtatagagt aagaatagat tcccctctat
     2161 ttcatatgca gaaccggaat aagcgccgtg gctatctcgt tttgtgtctg gaagaagttc
     2221 tgctcctgtt acatgaaaat gaactaaatc atctgattca ttgtgtaccc attctttcaa
     2281 cccatgagca gctccaaatg gccaattttg ataaaaaagc gtatattttc cgttaaaaaa
     2341 agaaaaacca ttaggatcgt ttaacaatcc tgttttaggt tcgatatgat agctagcacg
     2401 ccaaggtgat cgttttacat tatccttaat tttatccatc tcatctttag tccaatcaga
     2461 ataagcacga tatcgtattt ctcgaggtaa gttcatattt tactccttca atttatcttt
     2521 ttacaactag tatggtaaac gttttccgtt gaaaaatcaa tcatttcatt taaaaaaata
     2581 aactatatga caaacgtttg acatattttt aagatatgct aaaataaatt tgcatttcag
     2641 tgaaccgttt tcacgtaaaa ataaaaaagg agtgattcga atgaattaca gtaaagtagc
     2701 ttccgacgta atacaagcgg tcggaaaaga taaccttatt gcagctgcac actgcgctac
     2761 acgtcttcgt ctcgtactaa aagatgacac gaaagtgaat caaaaagctt tagacgaaaa
     2821 tccagatgtg aaaagcacat ttaaaattaa tggacaatat caagtgatta tcggtgcagg
     2881 tgatgttaat ttcgtttatg acgaacttat caaaaaaact ggactttcgg aaatttcaac
     2941 agatgattta aaacaaattg ctagtaaaga taaaaaattc aaccccatta tggcattaat
     3001 taaattatta tcagatattt ttgttccaat tatacctgcc cttgttgcag gtggtttatt
     3061 aatggcatta caaaactttt taacagctgc tggacttttt ggaccaaaat caattgaaga
     3121 aatgtatcct gcgattgaag gtatttcctc aatgatccaa ctgatgtccg ctgcaccatt
     3181 catgttctta ccaattttag taggtatttc agctgctaaa cgttttggag caaaccaatt
     3241 tcttggtgct gcaattggta tgatcatgac aacaccagat cttggtggct caactgaata
     3301 ttggaatatt tttggttatc atgtttcaca aacaaactat gcttatcaag ttattccagt
     3361 tttagcagct gtttggatat tatctatatt agaaaaattc tttcataaaa aattgccatc
     3421 atctattgac tttacattca cgccattatt atcagtaatg attactggat ttttgacttt
     3481 cacagttatt ggccctgtaa tgttattgtt gtctaatgga attacagaca ttattgtttg
     3541 gctatataac actactggat ttattggaat gggtattttt ggaggaacat attcacttat
     3601 agtcatgact ggtttacatc aatcatttcc agctattgaa actcaattac tttcagcgtg
     3661 gacaaatggt atcggacatg gagactttat ctttgtagtt gcatctatgg caaacgttgc
     3721 tcaaggtgct gcaacatttg ccatttggtt cttaactaaa aattctaaga caaaaagtct
     3781 tgcttcctct gcgggtgtct cagcacttct tggaattaca gagcctgcct tgttcggagt
     3841 taacttaaaa taccgctttc cattcttttg tgctttaatt ggttcaggaa ttgctacagc
     3901 agtagcagga ttacttaaag tagttgctgt ttcacttggt tcagcaggtt tcttgggatt
     3961 tctttcaatt aatgcaacat ctattccatt ttacgtattg tgtgaattga ttagctttgt
     4021 agttgctttt gcaattactt acttctacgg taaaacaaaa gctgctggta tctttaaagc
     4081 agaagaaatt gctgaacaaa caccagttaa ttcttcaaaa gtagcaacaa cagatccaat
     4141 atctactgat gttgaatcaa cacaaacaaa agtttcatca gaaacaattg ttagtcccat
     4201 ttctggtaaa gtgattagtc tttcttcagt aaatgatcca gtcttttcat cagaagctat
     4261 gggaaaaggg attgccatta aaccaaatgg aaacacggtc cattctccag tagatggtac
     4321 tattcaactc acgtttgaaa ctggacatgc ttatggtatt aaatcaaata atggtgctga
     4381 agttctaatt catattggta ttgatactgt atcaatggga ggaaaaggat ttacacaaaa
     4441 agttactact aatcaaaaag ttagaaaagg cgatattctt ggagcatttg accgttcaat
     4501 gattgagaaa gaaggtcttg atgatacaac aatgattatt gtgaccaata cttcggatta
     4561 ttcagaagta aatatagtag ctgaaaatac agtcactgag ggtactgatt tacttgaaat
     4621 caaataaata tatttccctg agtaaagatg gaattttctt tccaaaatca aataatatag
     4681 attaatcaaa tatttgagaa taacatttta tttaatcttt tcatatatca attgtatgaa
     4741 aaattattag aattttaaaa aatgataaaa attcaaaaag aaaaaatagt agttatttac
     4801 ttgtcatgtt actaaataaa aaaataattt aaatatgata tatagtcatt tatttagtga
     4861 tttcattcta aaataaaggg agaaatgtct atgctatacg gtgcaattga agcaggcggc
     4921 acaaaatttg tgtgtgccat tggcgatgaa gaaatgacga tcaaggaaag agtgagcttt
     4981 ccgacaacga cacctgagga aacaatgcct ttagtcattg acttcttcaa acaataccaa
     5041 gcagatttag cagggattgg gattggctca tttgggccta tcgatatcca tcgcgattcc
     5101 gccacttatg ggtacattac ctcgaccccg aaattggcat ggcaaaattt tgatttcatc
     5161 ggtaccatga aaaaagaatt tccgatcccg atttcttgga caacagatgt caatgcggct
     5221 gcctatggtg agtatgtctt cggcagtggc aaaggattga gcagtgtcgt ttactatacg
     5281 atcggtacgg gtgttggcgg cggtgcctta caagaagggc gctttatcga agggtttagt
     5341 catccagaaa tggggcatat gctcgtcgtc cctcatccaa aagatgactt tgctggcagc
     5401 tgtccatttc atggcaactg tttagaaggg atggccgctg gacccgccat cgaaaaacgc
     5461 ttagggaaaa aaggacaaga ggttgcagaa gatgatcctt attgggaaat cgaagcctcc
     5521 tatattgctc aatgtgccta caatacaacc ttgatgtttt ctccagatgt gatcattttt
     5581 ggcggtggtg tgatgaaaca acgccatatg ctgcaaaacg tccacgacgc atttgcaaaa
     5641 ttggtcaatg ggtatgtcga aacacctgag ttaagcaaat atatcgtgac cccagcatta
     5701 gaagacaacg ctggaacgct cggttgtttg gcattggctc gagaagcggt cttacattca
     5761 taaacgaaaa aaggctcttc gacatttagt gttggtagag aaagatacaa ctgaaaattt
     5821 agtcataacc agttaggcag agtgcttaac tggtttttgt tttacttgga agatcaagcc
     5881 ttgaatgaga taaattcttg ctcgaaagtt atggaaattt cgatagccgt aggccacttg
     5941 tattgtgcaa tagtttttta cacgccgttt gtataattaa gcgagacaaa taaaaaagcc
     6001 atttatgtta cactcaaatc gtaatacccg ctaaagcata caaggagagt gaacataaat
     6061 gacttatacc catcttacac caaacgagct tgtaatgata gaagcatatt ttcatcaaga
     6121 aactccggtt gctatcgttg cgaagcagct taaacgtgga cgccaaacaa tttacaatgt
     6181 ctataacttt ctcaaatgtg gtggaacagc acttgaatgc tttgaacaat acaaagaaaa
     6241 taagcgacgt tgtgggagaa ccgaaatcat ttttcctgct gaggaaaaag aatacattgc
     6301 aaaaagatca actgaaggtt ggaccccaga cgtcattatt ggccgtgcag agcgaacctt
     6361 ctcttgttca gtaagtaccc tctatcgccg gtttaagacg gg
//
LOCUS       DARDHA010000147         6303 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000147 DARDHA010000000
VERSION     DARDHA010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.149"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            334..714
                     /locus_tag="SDA10_002335"
     CDS             334..714
                     /locus_tag="SDA10_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEG4241216.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="SDA10_002336"
     CDS             696..1610
                     /locus_tag="SDA10_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEG4241217.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="SDA10_002337"
     CDS             1855..2544
                     /locus_tag="SDA10_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241218.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="SDA10_002338"
     CDS             complement(2665..2751)
                     /locus_tag="SDA10_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG4241219.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="SDA10_002339"
     CDS             3427..4377
                     /locus_tag="SDA10_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HEG4241220.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="SDA10_002340"
     CDS             4398..4931
                     /locus_tag="SDA10_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG4241221.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4947..5117
                     /locus_tag="SDA10_002341"
     CDS             4947..5117
                     /locus_tag="SDA10_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241222.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5567..6100
                     /locus_tag="SDA10_002342"
     CDS             5567..6100
                     /locus_tag="SDA10_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241223.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DARDHA010000148         6187 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000148 DARDHA010000000
VERSION     DARDHA010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.151"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            134..250
                     /locus_tag="SDA10_002343"
     CDS             134..250
                     /locus_tag="SDA10_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEG4241224.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            266..1069
                     /locus_tag="SDA10_002344"
     CDS             266..1069
                     /locus_tag="SDA10_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG4241225.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            1304..1540
                     /locus_tag="SDA10_002345"
     CDS             1304..1540
                     /locus_tag="SDA10_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241226.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1547..3499
                     /locus_tag="SDA10_002346"
     CDS             1547..3499
                     /locus_tag="SDA10_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEG4241227.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3571..4068)
                     /gene="dfrG"
                     /locus_tag="SDA10_002347"
     CDS             complement(3571..4068)
                     /gene="dfrG"
                     /locus_tag="SDA10_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEG4241228.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            4430..5470
                     /locus_tag="SDA10_002348"
     CDS             4430..5470
                     /locus_tag="SDA10_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG4241229.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            5568..5756
                     /locus_tag="SDA10_002349"
     CDS             5568..5756
                     /locus_tag="SDA10_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEG4241230.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(5814..6167)
                     /locus_tag="SDA10_002350"
     CDS             complement(5814..6167)
                     /locus_tag="SDA10_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4241231.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
BASE COUNT         2137 a          991 c         1115 g         1944 t
ORIGIN      
        1 taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagcg
       61 ggaacaaata attagatgtc cttttttagg agggcttagt tttttgtacc cagtttaaga
      121 atacctttat catgtgattc taaagtatcc agagaatatc tgtatgcttt gtatacctat
      181 ggttatgcat aaaaatccca gtgataaaag tatttatcac tgggattttt atgccctttt
      241 gggtttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
      301 tgatgcagga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
      361 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
      421 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
      481 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
      541 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
      601 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
      661 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctgccgaaat
      721 tgtaatcaaa cagaaggtag aactgtatcc taatatgtgt gtgacgaact ttaccgaatc
      781 tgaacaatgg gatacggtaa tagagggaaa cgatgacctt ttagagaaat atatgtccgg
      841 taaatcatta gaagcattgg aactcgaaca agaggaaagc ataagatttc agaattgttc
      901 tctgttccct ctttatcatg gaagtgcaaa aagtaatata gggattgata accttataga
      961 agttattact aataaatttt attcatcaac acatcgaggt ccgtctgaac tttgcggaaa
     1021 tgttttcaaa attgaatata caaaaaaaag acaacgtctt gcatattaag tagttcagtt
     1081 ttggagtaca aaaagtgaaa atgtaaacct aaatgagtat caaattgcaa ctgaatttga
     1141 tactcgtttt ttattgttaa atcaacgttt gttgttaatt tacaccacaa aatgtaagtt
     1201 aaaatgtata taaaaatgcg aacgtgaaat aattattgta ggaatgcaaa ttggtgtgtt
     1261 aaaatggtta caatttacta agcaaaaagg ggtgcatttt cctatgcaat ttcttgagcc
     1321 aaaaaataag aacgctaagt ctgtggattg ggaaatatca gagcaagtaa gggtgatagt
     1381 taagcaatac gctgaatatg cagagcgtac tgaaagcgaa gcagtggatg aattcttatt
     1441 gaacatttta gacgataaga agtttattga atggattgcc aacaagcgaa gtaataagcg
     1501 gattgttgaa aaaatgggta tcaaagatag agtgggttga tagacaatga ccaagcttaa
     1561 aagattagcc acgaaggaag atgaaattgt agatgttaaa attccgattt caaacgaaga
     1621 attaaaagat agggctaaac aatatgattt attaactcca aaacgatttg caacaaggta
     1681 taacaaaatg ttgttcttgc caacgtcttt taaatggaac ggttctgaat atcccattca
     1741 atataattac tgcatcaatc cgttttgttg taattttggg aaagaacagc acaaattcaa
     1801 agatgtaaaa gggaaaccaa gtcgatataa aatgactggt agttccaaag ataaaggtca
     1861 taaaggtatg tactgcaatg acaatcctat tggcagaggt gtatcgcaaa actgtacagt
     1921 aaccccttta tctaattggt ctgttgttga agaaattaaa cgccttatag aaatcaatag
     1981 catccaagat gttgagccag attatcaatt tcataaagaa ggctgctcgg aagaagaatc
     2041 aaccccattc aatgagccga agcaatttta caaaaggggg aaaagtaggg gcaaatctca
     2101 acgctatcaa tgtaaggcgt gtaagaagtt cactaatgtt ctaccaaaaa gagaagaaac
     2161 cactacttat caccaacaaa agaacaccat tcttccaatg ttcgctaaga tggttgttgg
     2221 aagggtttca gtcagtcgta cttgtgatat tttaggaatt ggagttggaa cctactatca
     2281 taagctggag tggctgtata ggcgttgttt ggagttttta gaacgatatg agacacaacc
     2341 ccttcaaaca aagaaattta acgagatgtg gcttaatacg gacaagatgc actactacct
     2401 aaataacgtc cgtaagaaag gacaaggttc aaagaaatat acggggtttg aagaccttaa
     2461 tatgcaaacg tatatagttg tatctgctga ggtcttatca agatatgtgt tccgttctga
     2521 tgttgcctat gattggaata tttcaatgga tgaattaaat gaagatacaa gaaaattcaa
     2581 agaagaccat ctcaatactt ttagtcgtaa aaatgatagg ttagattggt cttattatcc
     2641 acaagaacca tccgctaacg atagcgaaaa taggaatgct tatcttcacg aattagggaa
     2701 aattactaat aggagtagat ttgtcgatgg tttaaatgtt gatgctcctt atactacaac
     2761 tgctcactat tggttaatta aacaaatggt taatgccgac gaatggcgaa tgattagtga
     2821 tgatgacttc tctattagaa acgcctttta tagagtattc acgaaagaat taaggttgag
     2881 cgatgcacat cattttattt gccaagttaa caagacgaaa agcagaaaac aatgcttaaa
     2941 agaattcgga caagcaaaag cagaactgct cgattgggga gatatcagag gattcaaaac
     3001 aaagtttcta cggactttgg catctcatta tctaactgaa ttactaacaa gtcatcaatt
     3061 ccacgaagag gctattagta aagatggaga aagatatcgc aaatatgccg ataaccctat
     3121 caaacatcca ttagccacta aggataaagg attttattca gttgattgta gaacggattt
     3181 atctgctctg gaaccaaacg aaatcgcaaa gatgctatta aatgttaatg accattcgac
     3241 caatagcttt attcaacaga taagacgtta tatatcatct cttgaaagac cattaacaac
     3301 agctcgtggt gataagaaga gctatattta cgctaacttc aatccgaagt atgctcaatt
     3361 tgcgattaca attctaagaa cttactacaa cttttgtcgc ccttttaaat cagcggataa
     3421 aaaggttttg acacctgctc aaagattagg gattacagat aaacaatttg attggaaaga
     3481 tataatttat ttcaaatgac aaaaagagct tggagtaatc ccaagctctt gtgctttatc
     3541 tttactgttc ttcttcaatt aacgcacccg ttaactcaat aagttttttc tttcatatac
     3601 atgaaaatag tagttatacg gatttttatc attctttatc cctttttggg caaatacctc
     3661 attccattcc tcataattca cttctggaaa aaaagtatct ccttcgaatt catgatgtat
     3721 ttttgtgatg tacattttct caacataagg gaaaaacaaa ttataaatct gttctcctcc
     3781 gaaaataaaa atttcttctt cgtttttaca taactcaaaa acatcttcta ttgaatgaac
     3841 aatttcacaa ccattaaagg taaacccctt atctctcgtc agaataatat ttcttctgtc
     3901 aggtaaggct cttccgattg attcaaggtt cttcctacct aatattatcg gatgtccctt
     3961 tgtagtattt ttaacatatt cccagtcctt gggaatcctc caaggaatgt cattctcttt
     4021 gccaatcact ctattcttat ccatcgcagc aatcaaagaa actttcattt ataacacagc
     4081 ctttttaact aatttaccat ctaatttctt atttctatca aaaaaccttt tctcattatc
     4141 ctgcccgtta gttgaataag caattttttc tataagttat attatcataa taccacaaaa
     4201 tcacaaattt tcattcaatt atcattttca attgcatttt ataaatcatt ttgtatgtaa
     4261 tttggtatgt aagttaaaat gaaaaaagta gtccattttg aagtacattt tgtcattttg
     4321 tactccaaaa ctgaactact tacttgcata tatacgcctt tatagtggag tactacattt
     4381 acgagattcg gttagagtat cagaaaaaga aaaaataaaa gttacagaaa tgtatacttc
     4441 aataaatggt gaattatgta agattgatag agcttattct ggagaaattg ttattttgca
     4501 aaatgagttt ttgaagttaa atagtgttct tggagataca aaactattgc cacagagaaa
     4561 aaagattgaa aatccgcacc ctctactaca aacaactgtt gaaccgagta aacctgaaca
     4621 gagagaaatg ttgcttgatg cccttttgga aatctcagat agtgatccgc ttctacgata
     4681 ttacgtggat tctacgacac atgaaattat actttctttc ttagggaaag tacaaatgga
     4741 agtgattagt gcactgttgc aagaaaagta tcatgtggag atagaactaa aagagcctac
     4801 agtcatttat atggagagac cgttaaaaaa tgcagaatat accattcaca tcgaagtgcc
     4861 gccaaatcct ttctgggctt ccattggttt atctgtatca ccgcttccgt tgggaagtgg
     4921 aatgcagtat gagagctcgg tttctcttgg atacttaaat caatcatttc aaaatgcagt
     4981 tatggaaggg atacgctatg gttgcgaaca aggattatat ggttggaatg tgacggattg
     5041 taaaatctgt tttaagtacg gtttatacta tagccctgtt agtactccag cagattttcg
     5101 gatgcttact cctattgtac tggagcaagc ctttagaaaa gctggaacag aattgttaga
     5161 gccatatctt agttttaaag tttatgcacc acaggaatat ctttcacggg catataacga
     5221 tgctcccaaa tattgtgcaa atatcgtaaa tactcaactg aaaaataatg aggtcattat
     5281 tattggagaa attcctgctc gatgtattca agattatcgc aatgatttaa ctttttttac
     5341 aaatgggctt agtgtttgtt tagcagagct aaaaggatat caggttacca ctggcgaacc
     5401 tgtttgccag acccgtcgtc taaatagtcg gatagataaa gtaagatata tgttcaataa
     5461 aataacttag tgcgttttat gttgttatat aaatatggtt tcttattaaa taagatgaaa
     5521 tattctttaa tatagatttg aattaaagtg gaaaggagga gattgttatt ataaactaca
     5581 agtggatatt gtgtcctatt tgtggaaata aaacaagact acgaatacga gtggatacta
     5641 tacttaaaaa tttcccttta tactgcccca aatgtaagaa cgaaacttta attaatgttc
     5701 aaaaaatgaa tataataaca atcaaagagc cagacgccaa gacgcagagc cgataatttg
     5761 agaaatgaaa ctctcatctt atcggctctt tttgtttatc tgaattttac tgactagcct
     5821 tcaatatttc ttgcttcatt gatacctttt gctagtgctt ccattaagga tagttctttg
     5881 tctgtaaagc tatccatgta tttttctacc tgtaaccttc gagtgctttt aatcaaatta
     5941 tctgtaggta agaaaaattc atcaatagat acatggagta atgatacaag atcatacagc
     6001 acttgtgtac ttgggtgttg tcctttgttt tctatatttg ttaaataacg tgggtcaatt
     6061 tcaatcattg ttcccacttg ttcacgagtg agaccccgtt tcattcgagc ctctttaatc
     6121 gctaaaccaa cggctctaaa atcgtattta tcttcttttt tacgcatact aaatcacctc
     6181 tatacat
//
LOCUS       DARDHA010000149         6173 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000149 DARDHA010000000
VERSION     DARDHA010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.152"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            725..811
                     /locus_tag="SDA10_002351"
     CDS             725..811
                     /locus_tag="SDA10_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG4241232.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="SDA10_002352"
     CDS             complement(932..1621)
                     /locus_tag="SDA10_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241233.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="SDA10_002353"
     CDS             complement(1628..1906)
                     /locus_tag="SDA10_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241234.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="SDA10_002354"
     CDS             complement(1866..2780)
                     /locus_tag="SDA10_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEG4241235.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="SDA10_002355"
     CDS             complement(2762..3142)
                     /locus_tag="SDA10_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEG4241236.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="SDA10_002356"
     CDS             complement(3592..3879)
                     /locus_tag="SDA10_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG4241237.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="SDA10_002357"
     CDS             complement(3899..4123)
                     /locus_tag="SDA10_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HEG4241238.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="SDA10_002358"
     CDS             complement(4578..4748)
                     /locus_tag="SDA10_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241239.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="SDA10_002359"
     CDS             complement(4763..5296)
                     /locus_tag="SDA10_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG4241240.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="SDA10_002360"
     CDS             complement(5317..6159)
                     /locus_tag="SDA10_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG4241241.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1152 c         1042 g         2346 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaacaaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DARDHA010000150         6147 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000150 DARDHA010000000
VERSION     DARDHA010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.153"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            102..2543
                     /locus_tag="SDA10_002361"
     CDS             102..2543
                     /locus_tag="SDA10_002361"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEG4241242.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2873..3577)
                     /locus_tag="SDA10_002362"
     CDS             complement(2873..3577)
                     /locus_tag="SDA10_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241243.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3764..4009)
                     /locus_tag="SDA10_002363"
     CDS             complement(3764..4009)
                     /locus_tag="SDA10_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241244.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4026..4877)
                     /locus_tag="SDA10_002364"
     CDS             complement(4026..4877)
                     /locus_tag="SDA10_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG4241245.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5043..6047
                     /locus_tag="SDA10_002365"
     CDS             5043..6047
                     /locus_tag="SDA10_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEG4241246.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         1970 a         1064 c         1301 g         1812 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
     3061 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
     3121 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
     3181 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
     3241 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
     3301 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
     3361 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
     3421 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
     3481 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     3541 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     3601 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     3661 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaaaa
     3721 atggaacgga ttatgtatat atacataatc cgttccattc tttctatttt ttcggtatca
     3781 aggcaaaaac aggtagttca ggtgaataaa tgacttcatg ttcgtgccag ggtttgacca
     3841 ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat ttgatcatgt gtccagtttc
     3901 ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa gacaaaatcg gaatgaaaag
     3961 tctgggtaac ttcttccgag agagaattca agcgttcgat tggatccata aaatcacctt
     4021 tcgacttaaa attgggtagt atctgggtct tttgctttgc cgactacacc atctaactga
     4081 tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa ataattcagt attttctttg
     4141 attctgcttg gtgtcactga cttaggaaga ggaaggaatt cgtgttgcag cgaccaacgc
     4201 aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga tttgtttcat ttcaggcaca
     4261 tcgaagattt taccagtgcc taatggacta tatgcttcga ggatcatatc atgttttttt
     4321 gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa ggaaaacttg attgaccatc
     4381 ggcataattt ttgctgtttt tgctagtgta tctagatggt ggggtaagaa attgctcaca
     4441 ccgattgctt tgattttacc tgcttcatac agttcttcaa atgctcgcca tgtatctgca
     4501 tttgcttgtt cccaattatc acgaaaagca acagggtttg gccagtggat caaaaacaga
     4561 tctaaataat ctgtttggag cttcttcaat gattcttcaa aagaagacat cacaagttca
     4621 tagctgtggt ttgcattcca taattttgtt gtcaagaata tctcttctct aggaattcca
     4681 ctttccttaa tcgcttggcc gacgctttct tcattcttgt atccttgggc agtatcgata
     4741 tggcgataac ctgcagccag cgcttcttta acagaggaaa cagcgacatc cccatcaggt
     4801 gtttgccatg tgccgaatcc tactaccggg atttcgtaac catttgatag acgataagtg
     4861 cttgttaatg aattcatact ttcagctcct tttttattcg ttcaactata agtgtagcgc
     4921 atttttcgaa aaaaagaatc cattttgttt ctactcttga aatttttgtt taaaagaaaa
     4981 tgattttgat tctggtattt ttgatttttt ttccgtaaac taagacagaa gggaatgaga
     5041 aaatggatat ctatttaaaa aaagcaattc tgcatattat tgacagagaa actggttctc
     5101 cagtcttttc acaaaaagag ctggatctga caaaagaata tattcgtgat tttcttcaaa
     5161 agaagatcca aaaaatctct tctgctcaaa caaaaactgg acaactagca gaagaaagca
     5221 tgatcgttga atcacttaaa atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg
     5281 ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc acctagtgca gatgtatttg
     5341 ttgtcttgta tgaattagat acagtgatgt acttatcttt attgaaagtc aattatcgtg
     5401 aggcttacac tcactttgtt gaagcagacg aagcagggat cgacaaccgc ttgattttga
     5461 atcgagcaat tctaggggcg aaaacacaaa aagcagatga agcattcgct gtgaatttga
     5521 ctgattggac ctacgaatta attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt
     5581 atttttctgg aagagtgatc gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag
     5641 tgatccaaaa agtagccaag catcttggaa aaaaatttga gacagaagaa tttgatatca
     5701 tggctgattt gaaagaagct gtctatgata cgatagaaga aaaaggtcgg ttggatcatg
     5761 agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc aaaaatggct tttaaagaag
     5821 aagtacaaga gcaaggattt gtcccagaag cacctcctgt aaaagaagta caagaaattt
     5881 ctgagaaaaa atttggaaaa caaaaattca agctatcaaa cgggatcgaa ctgatcgtac
     5941 cagtagatgt atatcgcaac ccagatttga ttgaatttgt gaataatccg gatggtacga
     6001 tatccgtgat gatcaaaaat gtggatgaag tattgaatcg tttataaaaa tgactagaat
     6061 attaggtgtt caagtaccag tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa
     6121 agacgaatat aacaaaaaaa gagcgtg
//
LOCUS       DARDHA010000151         6002 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000151 DARDHA010000000
VERSION     DARDHA010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.154"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..395
                     /locus_tag="SDA10_002366"
     CDS             <1..395
                     /locus_tag="SDA10_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241247.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="SDA10_002367"
     CDS             398..1228
                     /locus_tag="SDA10_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEG4241248.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="SDA10_002368"
     CDS             1244..2095
                     /locus_tag="SDA10_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG4241249.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="SDA10_002369"
     CDS             2092..2451
                     /locus_tag="SDA10_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HEG4241250.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="SDA10_002370"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="SDA10_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="SDA10_002371"
     CDS             2624..2929
                     /locus_tag="SDA10_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241251.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="SDA10_002372"
     CDS             2929..3240
                     /locus_tag="SDA10_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEG4241252.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="SDA10_002373"
     CDS             3237..3410
                     /locus_tag="SDA10_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241253.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="SDA10_002374"
     CDS             3407..3823
                     /locus_tag="SDA10_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HEG4241254.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="SDA10_002375"
     CDS             3823..4491
                     /locus_tag="SDA10_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEG4241255.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="SDA10_002376"
     CDS             4488..4865
                     /locus_tag="SDA10_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241256.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="SDA10_002377"
     CDS             4862..5161
                     /locus_tag="SDA10_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241257.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="SDA10_002378"
     CDS             5151..5309
                     /locus_tag="SDA10_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241258.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="SDA10_002379"
     CDS             5403..5819
                     /locus_tag="SDA10_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEG4241259.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DARDHA010000152         5920 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000152 DARDHA010000000
VERSION     DARDHA010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.155"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(158..2329)
                     /gene="copB"
                     /locus_tag="SDA10_002380"
     CDS             complement(158..2329)
                     /gene="copB"
                     /locus_tag="SDA10_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HEG4241260.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(2349..4535)
                     /locus_tag="SDA10_002381"
     CDS             complement(2349..4535)
                     /locus_tag="SDA10_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEG4241261.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(4535..4744)
                     /gene="copZ"
                     /locus_tag="SDA10_002382"
     CDS             complement(4535..4744)
                     /gene="copZ"
                     /locus_tag="SDA10_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEG4241262.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4757..5197)
                     /locus_tag="SDA10_002383"
     CDS             complement(4757..5197)
                     /locus_tag="SDA10_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEG4241263.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5271..5810)
                     /locus_tag="SDA10_002384"
     CDS             complement(5271..5810)
                     /locus_tag="SDA10_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG4241264.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         1548 a         1323 c         1037 g         2012 t
ORIGIN      
        1 ggtatacaaa aaaattctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
       61 agaatctact tctggaatgc cgagaaccag ctgattgagt atcgattact cgaaaaaaag
      121 gttgtgacac gttttttgtc acaacctttt ttcattatta tctgattttc aatgtcaaag
      181 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
      241 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
      301 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
      361 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
      421 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
      481 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
      541 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
      601 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
      661 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
      721 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
      781 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
      841 tcacttgttg ttttttgact gttgcctcta gcccgactcc tgaaagattt tttagatttt
      901 cagcttgata aggcttgatt tcatgttctt tcaaataatt cataatgcca acagccaatg
      961 gatgattggc attcgcttcc aaggctccta gatattgaag gatttcattt ttattgaacg
     1021 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     1081 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     1141 gtaatccgtt tttagctgct attgatgtgg aacgagcaac aactaatggg atagctaaac
     1201 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     1261 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     1321 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     1381 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     1441 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     1501 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     1561 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     1621 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttc actgattctg
     1681 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     1741 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     1801 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     1861 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     1921 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     1981 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     2041 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaattcc
     2101 ccatgtgcat ggaatgatcc atgccgccgt gttcgtgatc catgtgcatc gaatggtcca
     2161 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tgatccatct
     2221 gactatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     2281 ctacttcttt tttatgctca tgttttttct ccatatcgtt cccattcatt tttactccat
     2341 cccttctctt actttttcat gtgtcgattt aagcacaggg agttcaacaa tacactgacg
     2401 gaactaaacg ccatcgctcc tcctgcaatg attggattca aaaagcctag tgcggcaaaa
     2461 ggaataccaa tcgtattata gacaaaagcc cagaaaagat tttgtttgat ttttctcaat
     2521 gtcaacctag acaactcgat tgtttgtgca atggatgcca aactgctatt catcagcgtc
     2581 acatcggctg tttccatcgc aatgtccgtt ccacttccca ttgccatacc gacatcagct
     2641 aatgctaagg ccggtgcatc attgattcca tcgcctgcca taccgacttt tttgccatct
     2701 ttctgaagtt tttctacgta ggctgcttta tcctctggaa gtacttcagc aaagatatgc
     2761 tttggatcaa taccaacttg tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc
     2821 agcataaaaa catctaatcc tttattctgg agctgcttga ttgcttgttt gacttctagt
     2881 ttgatttgat cagccacggc aatcaacccg atcacttgct tttcatcagc taaaaacatg
     2941 actgtttttc cctgctgttc taattctaac gcgtgttctt ggtattcatc aaatgatagg
     3001 ttcaattcaa ttaatcgttt acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg
     3061 ctgattccag cacctgggtg ggctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc
     3121 agttttccgt attccacgat tgctttacct agtggatgtt cagaagcttg ttctaaagta
     3181 taaaaaatag ataggacttc tttagaaccg actagatctg taactttggg ctttccttct
     3241 gtaatcgttc cagttttatc caaaacaatg ctgtctaagt gagcagctgc ttctaatgct
     3301 tctccgcctt taattaaaat tccatttcgt gcaccaacac ccgttcctac cataatagct
     3361 gttggagtcg ctaaacctaa tgcacatgga cacgcaatga ctaatacaga gacactatgg
     3421 atcaatgcga gttgccaatc accagtgatc aaacctgtag ctatgagcgt gataaacgcg
     3481 atgactaata cgatcggaac aaaaatccct gagatccgat ctgcgatttt ttggattggg
     3541 gctttgctcc cttgagcatc ttccaccatt tgaatgattt gcgctaatac tgtctgcttg
     3601 ccgacttgtg taacttccat gtgaagtaga ccattcgtat tgattgtccc gccaaagagc
     3661 gtgtcatccg gattcttttc tacaggaaga ttttcaccag taagcatact ttcatctatc
     3721 gcactgcttc ctgaaataat tcgaccatct gctggaatct gctcaccagg acggatgact
     3781 agttgatcac caacgactac ttcttcaatc gctaacgttt cttctttccc gtttcttatc
     3841 acttgggcgg tttttgtttg aagagacatc aattgtttga ttgcattacc tgttttcgtt
     3901 tttgctgtat gttccaaata tttgccgagc aaaatcagcg tgatgatcat actgctgctt
     3961 tcaaaataaa gatcttgggg gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag
     4021 gcagccgatg ttccaattgc taccaataca tccatatttg gtgctttcgt cttgattgca
     4081 tggtacgcac ctttataaaa acgagcaccg acataaaatt ggacaggagc agacagcact
     4141 aactggacga ttggaaaatg aaaaaagtga acaatcgccg catggcttcc taacatcata
     4201 gcaatcattg acaacatcaa cggcaaagtc aaaaccgtac tcaatataag atcgaatagc
     4261 atttttttca aatacgcttg cttttcttct gcaatttttt gtttgtgggc ttcatcgtac
     4321 aaaattgcac catagccgat gttttctacg ctttggatta atttttcagt ggtcgtccct
     4381 tcatatttga cacttgcttt ttcagtggcc aagttgactg tggcattgac cacaccaggt
     4441 tgtccgttca gctctttttc gatcctagca gaacaatttg cacaagtcat acctgtaatg
     4501 acgaacgtat ccattttggt ttctgctgtt gccatcaaat cacctctgct ttataaccca
     4561 atccattgat tgcttgacaa atcttttcgg attgaacttt tgtttcatca aacttgaccg
     4621 taccgtttgc ttttttcaaa tttactttca ctttttgcac gccgtcaagt gcgcttactg
     4681 tttcttctac tcttgctgca caatgattgc agctcatacc ttcgattgaa aattgttgtt
     4741 tcattttttc ttcctcctat tgatgttgtt tacattcaca ttgacctggg atacagttac
     4801 attcgatcgt atctactggt tgcttttgtt ccagttgttt caaaatctca ttgatatctt
     4861 cttgagtcaa agtggcttct tctactagat ccgcaatcgt ctctccaatc ctttttgcac
     4921 agatatggga aaaaagattt tccgtcgcac tgcgaactgt ttccatctcc gagacagctg
     4981 gataataaat gaattttttt ccttcttgtt ctgtccagat cacttctttt tttaccaaac
     5041 gacccagcaa agttttgatc gttgctggtt tccaatccat cgaatcagct aagatctgcg
     5101 tgatttcttg ggctgtcgtc tgtccaagtg tccaaataac acgcatgatc tcccactctg
     5161 aatcactgat ctttactggc gttttgactt tttccattta tttcacacct ttcgtttaca
     5221 aatgtaatca ttatcttatg aatacagatt acacttgtaa acaaaaactg tcaattattt
     5281 ttgctctcta ttttcaattt agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg
     5341 atcagtttat ttcttgcatt agctacttgg atcagctgct gtattttcgg ttcattgttt
     5401 tcttcaaaat gccagataaa agataaaaat tctaagtatt ccctgtttag tttctccgaa
     5461 aatgattcag gcatatctgg ccgagatttt ttgtttatct ttctttttag agaacgccaa
     5521 actactcggt agatggcgag ataacgcggc actttaagcc aaatgatctt atctgcttct
     5581 ttaatccttt cctctaatgt agaagtataa tttccttcaa cgatccatga cggattagat
     5641 accataaaca attgctgttc ttgcaaaaac cacttttttg cctgaatact gtagtctgtt
     5701 gtatgccata aaaggtctag cggcagtagc ggaaagtcgg tgatttcttt gatcttcctt
     5761 gcgagggtcg attttcccga cccactttgt ccaatcacga tgatcttcat tcttctccct
     5821 cacttctctt tggaatttgg ttcgatcata ccacttataa cgccgcaaaa aaacaaggga
     5881 ctgtgacaaa agtcgtgtca aagtcccttg tttcgaataa
//
LOCUS       DARDHA010000153         5852 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000153 DARDHA010000000
VERSION     DARDHA010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.156"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            88..927
                     /locus_tag="SDA10_002385"
     CDS             88..927
                     /locus_tag="SDA10_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG4241265.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            1078..4095
                     /locus_tag="SDA10_002386"
     CDS             1078..4095
                     /locus_tag="SDA10_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG4241266.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            4159..5622
                     /locus_tag="SDA10_002387"
     CDS             4159..5622
                     /locus_tag="SDA10_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEG4241267.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
BASE COUNT         2226 a         1012 c         1208 g         1406 t
ORIGIN      
        1 aatctacaaa aaatatatcg actgatagac ataagtttgg tactatgttt ttgtaaataa
       61 atttattcaa atcggaggcg aaaaacaatg aaaatgttga aagtattggc actaggaatc
      121 gtagtcggag gagttagtct tgctgtggga acgaatgctg aagcagcaga aaacaaagtt
      181 tggacacctc gttcggcgga acaaatcaaa gcagaaatta aagggaatga atacacaatt
      241 gtctggggag ataccctaag cgcaatcagt caagcaacaa atattactgt ccaaaaatta
      301 gcggacatga ataagattac aaatgtggat ttaatctatg caggaaataa attagtattt
      361 aatggcaatg tagcaagtgt acaaaacagc gcaggtgaaa ctgtggctca aacagtgatt
      421 caagagaaag acaaagttga tccgaatcaa ccagtaggac aaggaacgac ttcaaataca
      481 caaacgacac caaatacaac gacaccaaca acaccagctg gagatcaaac aggaacaact
      541 ggtggtacaa caacgccaag tacaccagaa aacaatggtg gcactactca aacacctaca
      601 caaccaggta atggtggaaa tactggagga gaaacaactc cttctaaccc aagcacaggt
      661 ggaggaacaa caacgcctga agaaccaagc aatcctggtg gaggcgatca aggcggaaat
      721 ggtggcacaa cgacacctga agagccaaca attgtagttg gagagattgg taatagtgga
      781 aaggtattta acactgtaga tgaagcctat gattgggcta atgcagaggt tagcgatcca
      841 aattcgcagt ggtacaacaa aggttttatg gtggatgtta ttatttacag tgacgggtca
      901 caaaaacaca ctgtagattt ctactaaaaa taaaagaaaa gtaagtggtt gatgaagact
      961 agaattataa gttctagtct ttttttgttt tgaggaggat aaaaatgttt cggtgtcgaa
     1021 gtccataaga aattacttta gaagactgaa aaataaaatg aaaggaacga aaaaaatatg
     1081 tttaaaagaa tggcaacttg gttggcaatt ttcgttacat ttctcaatcc catgttagga
     1141 tctgtaacgg catttgcaga aacgataaat gaatcaaatt ctactgaaga agtaatgaag
     1201 actgaagaat caaaaagtag tgaagaagcg atcagtactg aaaccacaag tgaaacaaca
     1261 gaagagacaa ctacgcagga cacgacagaa gatgtttctc cgcccaatgt aatgactcaa
     1321 gaggtccctg aaggttatga gaaatacttt ataaccattg aagatattca aaaagcgact
     1381 tctttttctg aacaagaacg attggcgcaa gaatctgaag aatggcaagc aatcttggaa
     1441 aagaatgcac agacacgtgc taccaatcta acattgactg aaaatggtcg taaagatgtg
     1501 aatatgactc gaattactcc tgaaggaaca atgtatactc atgattacat ggttcgctgg
     1561 caaattgacg gagaagatgt tttttgcatt caagaaggcg caatcacgag tgcgggtatt
     1621 caatatgatg tgacaccact cgaaaattta attacgacaa gtgctattag aaaaaagatt
     1681 tctttaattg gttattttgg ctactattct agtggttcaa tggataacta tgtattagca
     1741 caaacatata tttgggaagt tttaggtggt tcatttgtta attatggaac tattggacaa
     1801 tctaattatg aagctttcaa acagactgta gatagaaaaa taaacaattt acttgaagat
     1861 ccttcttttg atagccaaac agtgacggtt aaagtagggg aagaagtaga agttaaggat
     1921 acaaaaggtg tgttttctca atgggatggg tcggttgttg aaaacacagc taacgttcaa
     1981 gtaagaaaat caggaaatag tttgtttata aaagcaaatc aaaactcaaa tgacggacga
     2041 atttctgttt cttcactgga tccaaattct agaggggtag gttcaagttt tgcctatgta
     2101 gatcctgttg ctcaaaatct aggaaagctt tatgtgccag atcctaatag agctggacta
     2161 actattaaag ttctaaaaaa tggcgacttc aaactaacca aactagacgc tgtcacaggg
     2221 aaaaaagttc caaatgtgac gtatgaaatc aaacgtcatg atacgggaga aacaaaacaa
     2281 tatacaacgg atgccaaagg ggaaatcgcc ttaaaagaaa tccctgcatt ctatgaaaaa
     2341 gacggaaaat atgttcaaaa taaattggat gtcaaagaag tcaaagcacc agctgggtat
     2401 attctagaca aacaaactca tacgattacg attgaaccaa acaaagaagt gacgttaact
     2461 gcaaaaaatg acttacaaca gttcattcca gaaggaaaga aagtcaaaga agtctataat
     2521 gcggaagaat cctggaatca aggcaaacct gtttatgata ccgttcctgc agaaaatatc
     2581 gaatttgata ttatcgccaa aacagatatt actgcgccag ataaagaaac agtccttgtg
     2641 aaaaaaggag aagtcttgga ttcagtaaaa actgacaaag acggacactg gaaggcaaac
     2701 aaaaaattct acaatggcga tcaaaacaaa taccaattga ttgaaaaaaa tgtgccagac
     2761 aactaccgtc aaccttctga agaacaaacc acgttctctg tggcctatgg cgataacacg
     2821 acgcctgtgc ttacgtttga attagatgtg attgacaact tgttaaaaac gggtgaagta
     2881 gacttcaata aagtcgattc aatcaatcat ctacaattac caggagcgaa attcctgatt
     2941 gaaggtatga ctgcacataa taaaaatgtg tcctttgtgt atgaaacaaa agaagaacca
     3001 gaaaaactaa aattaccaga cggtacgtat aaatttacgg aaattgtgtt ccctgagcat
     3061 tatgggcaag cgtcaggaca aacagaaacg attttagtaa cggtcaaaga cggagaagta
     3121 caaccaatta ctgtgaaaaa cgaagaaatc gtggttccag aagaagacca accagaaatt
     3181 tctactttgt ttgtgacaac gacaggtgga aaagatttca acccgaaaaa agaccaagaa
     3241 ttagtggata aagtgtccca agtattccct aaatctgcgg ttgggacaga gaaatattgg
     3301 gtgacgcaat tccataaaat tactgccgat ggaaaagtca ctgtattaga tacggtaaaa
     3361 gaaacacgaa aagtgaccga tgaaaaagaa acatttgatg tgacgttcaa ctacaaagca
     3421 gacatggttt cagacggtga cacgattgtc gcaacgcaca ttgtctataa cgacaaagaa
     3481 gaggaagaag aattcgctcg tcattttgat ttaaccaata aagatcaaac agtgactgcg
     3541 aaagaaccaa aaatcgaaac acgctttatt acagtgaatg gcgaccaaac atttgatcca
     3601 gacaaagacc aagagttgat cgatcatgta tcgcaagaat ttccagaatc tacggtaggg
     3661 gaaaccaaat actgggtgac tcaattccat aagattgata aaaatggaaa agatacagtc
     3721 gttggtacgg tagaaactgc acgtaaagtt tctaaagcgt ctgaagagtt taaagtatcc
     3781 tttgactaca aagcaggcac gttaaaagac ggggagaaat tggtcgcaac tcatattgtc
     3841 tataaggata aagaccataa agaggaagaa gcacgtcact ttgatttgaa gaatgaaaaa
     3901 caaacattga cagcaaaagg cattaaaaaa gaaacgccga caacccctgt caaacaaacg
     3961 ccgacgagca caccaaaaca aagcttacca aaaacaggag aaacagtgaa cgatgtgtta
     4021 attgcattgg gactgtttct ttttgttggt gtgggggctg ttctggttcg tcgtaaaatg
     4081 aaacaaagca attaaagtat gactagagac agcaatcagc tgtctctaga tgattgattg
     4141 aagaatggag gcaatcttgt gaaaaaaacg agtaaatgtt tatctgcttt gttattagta
     4201 ggtcaagtat ttgggtatac gccagcggtt ttggctaatg aacaaggcaa caatcaactg
     4261 acaaattcgc aatctgcaac gatcactgaa gaaacgacaa caaattccac tggagaagaa
     4321 aatcaaccag aaacaccgac tgaagataca atagatacac cagataatcc agaaatgaac
     4381 gctccaccaa caggttctga agaacaacca gaaacaccga atcaaccaga caaaccagaa
     4441 gacaatcaac aacctgagga ggaaccgtca gaagaaccaa atgagcaacc ttctgatgaa
     4501 agctcacaga ctcctcaaaa accaagtacc gaaacagaag cggaaccgcc aacaaaaacg
     4561 gaacgcccac aaaaggcacc tgaacagagt gtgactgtac ctatgaatga cgtaccgcag
     4621 gcacaatcaa tcggttcaga agagacaaaa aactaccacc aagcaccaag cgcaccgata
     4681 caagaattag tcccttccag cacacaagat aacgaaggga ctattcattt tgaaaaagac
     4741 gcgtctgtag aaacgtttat ccgtaaaatc ggtgaatctg ctcgtaaaat tggacaagaa
     4801 aatgatttat acgcttctgt tatgatcgcg caagccattt tagaatccgc aagcggacaa
     4861 agtcaattgg ctcaggcacc aaactacaat ctatttggaa tcaaaggcac acataacgga
     4921 aagggcgtct cttttgcgac acaagaagat ttaggcaatg ggacacttta tacgacccaa
     4981 gcaacttttc ggcaatatga gagctatgaa gaaagcttaa aagactatgc caaactgtta
     5041 aaagaaggct tagttggaaa tacctctttt tatgatggtg tttggaaaag taaggcaaaa
     5101 acctatcagg aagcgactaa atttttaaca ggaagatacg caacagacac tagctacgat
     5161 aaaaaattaa atgggttaat cgaaacctat gacttaatca aatatgataa agaagtagct
     5221 ggtccacaac taagcaaaga aggctatatg gtacctctaa aaaattattc aatttctagt
     5281 tccttcggtt ggcgaggaga agaatttcat agaggattag acatggcagc caatcaagga
     5341 gaaccaatct atgcgagcaa ggcaggaacg gtcatagaag cttcgtatca tactttgtca
     5401 ggaaattatg tcacaattga gcatgaagac ggaagtgtgg ctctttatgc gcaccaacag
     5461 gaatattgcg tgaacgtagg acaaacagtt gaacaaggac aaattattgg ttatgtgggt
     5521 tcaacaggaa atagtacagg accacattta catttagaac ttgccttaga tggtagttta
     5581 acaaaagaca aattaatcaa cccagaaaca gtcttattct aaagaataag gctgtttctg
     5641 ataaggagtg gtatagaaaa tgtggttact ttttaaatat ttgatatatt tgtatctctc
     5701 attttcgttt tttaatttga aatctcgtat ctatctagaa cgtagtttat taaatatagt
     5761 attaggaata ttttttatcg tttgggtctt cattgactta tttctttact caaacgattt
     5821 ttattttatt tataaagaat ttaaaaatca gt
//
LOCUS       DARDHA010000154         5809 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000154 DARDHA010000000
VERSION     DARDHA010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.157"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            450..1604
                     /locus_tag="SDA10_002388"
     CDS             450..1604
                     /locus_tag="SDA10_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HEG4241268.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="SDA10_002389"
     CDS             1598..2020
                     /locus_tag="SDA10_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241269.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="SDA10_002390"
     CDS             3143..4075
                     /locus_tag="SDA10_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG4241270.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="SDA10_002391"
     CDS             4092..4607
                     /locus_tag="SDA10_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG4241271.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="SDA10_002392"
     CDS             4903..5184
                     /locus_tag="SDA10_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241272.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="SDA10_002393"
     CDS             5186..5602
                     /locus_tag="SDA10_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241273.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2228 a          961 c          930 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtatacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DARDHA010000155         5790 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000155 DARDHA010000000
VERSION     DARDHA010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.158"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(99..1229)
                     /locus_tag="SDA10_002394"
     CDS             complement(99..1229)
                     /locus_tag="SDA10_002394"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEG4241274.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="SDA10_002395"
     CDS             complement(1877..3079)
                     /locus_tag="SDA10_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG4241275.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="SDA10_002396"
     CDS             complement(3139..3342)
                     /locus_tag="SDA10_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEG4241276.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="SDA10_002397"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="SDA10_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="SDA10_002398"
     CDS             4879..5691
                     /locus_tag="SDA10_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4241277.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DARDHA010000156         5737 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000156 DARDHA010000000
VERSION     DARDHA010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.159"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(233..1015)
                     /locus_tag="SDA10_002399"
     CDS             complement(233..1015)
                     /locus_tag="SDA10_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241278.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1080..1916)
                     /locus_tag="SDA10_002400"
     CDS             complement(1080..1916)
                     /locus_tag="SDA10_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG4241279.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(1934..3343)
                     /locus_tag="SDA10_002401"
     CDS             complement(1934..3343)
                     /locus_tag="SDA10_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG4241280.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3357..5180)
                     /locus_tag="SDA10_002402"
     CDS             complement(3357..5180)
                     /locus_tag="SDA10_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4241281.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(5460..5687)
                     /locus_tag="SDA10_002403"
     CDS             complement(5460..5687)
                     /locus_tag="SDA10_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241282.1"
                     /translation="MIEIGYCLLIFTKKTSITIVAYIVGMLFIPSFTHQIHQFFPRAE
                     LLKLCDPLYAFLMLSRFWEFPAGYVGMVLLV"
BASE COUNT         1753 a         1252 c          942 g         1790 t
ORIGIN      
        1 aaatttctta aaaatttttt cactaaaatc actacctgaa acagtctaaa acacttatat
       61 atcaacgttt aatgaaacta tctccgtttc atttcagata caaataaata tagtcgggct
      121 ccaaatccga aaaaaaatac ccccagtagc ttatcctaag ctactggaga tgtataagta
      181 cggtcatata acaacggggt agggtaaata aatgaaaaag atggaagaat cattattcaa
      241 taactttttt aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat accacagaat
      301 aatctttttt cccattttca agtaaccgaa gtggaatgtc tttaccaact tgctctgtca
      361 ctgcattact gatacctgtt agattagcta ataaatctga taaagattga gcatctgtag
      421 aaatctctgt atcagtattt atcgtgattc ctttatcttc atttacaagc ttccaagtag
      481 gtggtatagc tgtgtatgtt tcgatgaatg aatcaattct ttcatctatc gtcttcccag
      541 tatctacttc tgtctcatta ctcttagatg aatcgattct ttcatccatc gtctgctccg
      601 taactgcttc tgtctcatta ttctcaccgg aaactctctt cacacccaaa cttatatatt
      661 gtccatcttt agtgtgcata gttaaaattc ccacgctttt actaataaat tcactataac
      721 cagttctttc aatagagact tcaatgtatt tcacactatc atactcaaaa gaaaaataga
      781 catgtaaaag cattttgttg tttacatcta cataataatc ctttgactct acttgagacc
      841 ttcctatacg gaatacagct ttactgtctg atatctctat accagcatcc ttattattaa
      901 tctgccaatc tccaagtagc tcttctggca agctagtagt ttcttgtgaa cactttacca
      961 cttcactggt agaggtcgaa acattcacct ccgcactgtt agaagaattt tccaaggaat
     1021 caggagcctt agcacaacca acaagtcttt atatattgtg cagccgatat tttcttcatt
     1081 cagtacaccc ttgaaatatg cagtgttaga taaagcagtt cgtcctgatt tgctgaggca
     1141 cccatctctt cttgaacgta ctccttgatt ttcaaggccg cccgatactc tttcggaaaa
     1201 cgctgcttca ctgaagccag caaaaaatca tccttctccg gcaactcttc cttcttaatc
     1261 attcgggcaa taaaaaactg taaatgcgtc acaaatctta agtaaagcat cgaatcttta
     1321 tcaacctttt tttcgggaaa cagatatcct agcatagcca agatatcctt tagtgctctt
     1381 accgagagca tggtttcttc catatttgct tgttcacttt gggcatttac aatgtgaaac
     1441 gcaatgtttc cagcctcctc ttctggcaac tgaacattca tttttttgtt gatcaattgg
     1501 atagcatagc agccgatctc aaattctttt gggtaatact tttgaatttc aatcagcagg
     1561 cgattttgaa aaacaatgcc tttttgactg cgttcaatgg catgcttcaa gtgatcaacc
     1621 aaggtaaaaa atagactttt attcagctta aagtcaaact tggcgttggc ataatcgata
     1681 attcgcttga cgcaaataat tagatcatcc ggagcagcct caaacagttt aatgtaatcc
     1741 ttttttagag catcagaaag tgaatctaat ttaaatactt tttggatttt gtcagccgga
     1801 accaaatcgc ctttctttag ttgaaatcca atccctttcc ccatcaaaat tacttctttg
     1861 ccttgcttat ctaagcaaag aattaaactg ttgtttaacg ttttaattac tctcattaga
     1921 ttcaccgttc tctctaagct aagccatttc ttgcgattac tttttgatac caaaagaagc
     1981 tttctttttt tattcgtttt aattctttaa gattctcatc ggttcggtca acatatataa
     2041 aaccataacg cttattgaag ccttgatggg tactaacaac atcaatcgct gaccaaggta
     2101 aataaccaaa aacatctaca ccatctgtta aagctgcttg aatagccaga atatgtttgg
     2161 ctagataatc aattcgatcc tggtcattaa ttcgtctatc ttcctcaact atgtcgggtg
     2221 ccccatagcc attttcagta attaaaatag gtaaatcata gcgatcgtac aatttcctca
     2281 atgttaatcg tagtccagta gggtcaacta cccagccata tggcgtttta tctacataag
     2341 gattttcagc tgcccggtag acaccttctt ctcccaacat gatctgttga tcccccgctc
     2401 tagcggaaac atctgatccg tcatttttgc tggcagcgat ggtggcagtt gaataataat
     2461 taatcgcaat aaaatcgggt ttaccggagg ataaaattgc ctgatcctct gcctcaattt
     2521 taggatataa tccacgatca ttcaaatagt tttcaaacaa ataattgtat tttccccgaa
     2581 ctgctacatc taagaaagac cagcctctca atgtttccca attatgggct gcaattgcat
     2641 cttctggact ggaggtagct tgatacatag ccgttaaatt gattgctgga ccgatttttc
     2701 ctttaagatt caactgatga tacaagtgaa ttactctagc ttgagctaat aaaacatggt
     2761 gattaatttg aaagagttct ttttttgaag gcagctctcc acttggtggc aaaccaatcg
     2821 ctccaggatg aaggatcatc gtgttttgct cattaattgt cagccaatag ttcaccttgt
     2881 caccatactc agtaagcaat acctgtacat agtccacaaa ataatcgata gttgctcgat
     2941 ttaaccaacc gccctgctcc tctagaaaat aaggtaaatc gaaatggtac atggtgacaa
     3001 tcggttcgat tccctgattc aggcattcat caattacttg atgataaaaa gaaattccct
     3061 tttgattggt tttattattt tcaatatcca taattcgcgt ccaagcgatg gaaaagcgaa
     3121 aagcttttaa gcctaattct ttaaacagct gtatatcttc tttatagtga tggtaaaaat
     3181 ccgaggcaac ttcaaaatta gctacccctt ctgtatgctg atacttgtca ataatagacg
     3241 gttgtttccc atcctcctga taggcaccct ctgcctggta ggcactaatg gaggcacccc
     3301 ataaaaactt ctctggaaat gctgaaagct gtttgtattg cacaaaaatc ctctccttaa
     3361 ctaatttgca tcaatggttc gtgttccttt aagcttccgc ttgcttcact aattcctgcg
     3421 taattttcac tgttggtcac taccacaatc actgtatcat cgtagccagc gtttctaatg
     3481 gcagcgaaat cggcgttgat taataattgt cctgctgaca cctgatctcc tgcttttaca
     3541 aatacttcaa agggttcccc ttttaaattc acggtgtcga tccccacatg gatcaataat
     3601 tcaatacctt ctttagaacg tagaccaatc gcatgtttag attctaaaat agtaatgact
     3661 tctccctcaa caggtgaata aatgctgttt tcttctggat gaatggctac gccctttccc
     3721 aatagtccct tagaaaagac agcatcgttc acttcagaaa taggacacac ggttcctttt
     3781 accggatttt caacaatgac tgcttcggct attgccgttt gctctgattc atcagaagta
     3841 tcgtcaaatc caattagata agcaatgata aaggttgcaa caaaggcgat tacacacgta
     3901 ataattgcgt gaacaatatt cattggattt tctccaataa aggctggtaa ggctgctaag
     3961 ccaggggaaa caaaggcata tctgactaaa ccgctcaagc cagcatacaa accagcaatt
     4021 gcaccaccaa tcatggaagc aactaatggt tttttcagct tcaaggttac tccatagagt
     4081 gaaggctcag taattcccat taaagcagtg atcccagtgg acacagctag ctgcttcaac
     4141 tctttgtttt tagttttcgc ggctacagca aaggtagcac ctgattgagc aatattagaa
     4201 gccagcattc ctggcccatt gatcatttca gaacccgtac tgctcaattg catagtagcg
     4261 attggcgtca ttgcccaagc tgtacccgta accaccaaaa atggctgcaa accacccatc
     4321 aaaaatggaa ttaaccaagc tgctttgccg ttaatccatt cagcccccat tgctataaga
     4381 tcgttcagca acgttccaaa cggtccaatg acaaccaacg ctaaaggccc cataattaaa
     4441 aataccagca tcggcttagt aaagaattta ataaacgaag gcgaaattcg atccgcaaag
     4501 ggctctacat aggacatgat ccaaacagta aagataatcg gtaaaactga tcccgcataa
     4561 tctgcagcta ataccggaat tcctagaaaa gaaacggcat cgccagtaga aaaaagctgt
     4621 gaaatatttg gatgaagcaa aatgccagca acagtaatcg ccaaaattgg gttgacatca
     4681 aactttatgg ctgccccata ggctaatagt actggcataa aataaaaggc tgcatcagca
     4741 atagtattca aaatgatgta agtttgacta ctgtctgata gtacaccagt caaagtcaag
     4801 acggccaaaa tggcttttac catcccggca cctgttatag ctggaatcat cggtgtaaag
     4861 gtggtagaaa tgacactaat caatcttgaa ataatacttt cttgattgtc tgaagagggg
     4921 ccctttttaa ttgaagttcc cacttccttt tgaatttcct tgaaggctgt ttggacttca
     4981 ttgccaatca ccacttggta ttgtccaccg ctatccacca cactaattac accaagtgtt
     5041 ttttctaagg ctttcgtatc ggctttcctt ttatctttga gttcaaaacg cagtctggtg
     5101 gcacaatggg ttaagctgac cacattttct tttcccccaa cctgttgcaa tacatttctt
     5161 cctacttctc tgtaatccat tattctctct ccttaaagca gtaaaaaaga ccaagtcaaa
     5221 ccacgcccca atcaacattg aagtgttagt ttgtcttggt cttgcctgca taatcagtaa
     5281 catgccaata acgttatttt gctgtaatcc aataatagca cattatgata gcgcaatcaa
     5341 cagggaattt aattttttat cttttcgttt gaccgtatag ggtaatataa cagttagatc
     5401 acatcaacta gggtatatct gcgtatactt actgctaaaa taagtatatc taatattaac
     5461 tataccagta aaaccattcc tacataacca gcgggaaatt cccaaaatcg tgaaagcatc
     5521 aaaaatgcat ataacggatc acacaacttc aacaattctg ctcttgggaa aaattgatga
     5581 atttggtgag taaaggatgg aataaacagc attccaacaa tataggcaac aatagtgata
     5641 cttgtttttt tggtaaaaat cagtaagcaa tagccaattt caatcattcc gagatataaa
     5701 ggtatcgata gaatgatttg ggctagtatt cttaaaa
//
LOCUS       DARDHA010000157         5731 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000157 DARDHA010000000
VERSION     DARDHA010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.160"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            362..1873
                     /gene="gadC"
                     /locus_tag="SDA10_002404"
     CDS             362..1873
                     /gene="gadC"
                     /locus_tag="SDA10_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HEG4241283.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            1886..3286
                     /locus_tag="SDA10_002405"
     CDS             1886..3286
                     /locus_tag="SDA10_002405"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HEG4241284.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(3451..4891)
                     /locus_tag="SDA10_002406"
                     /pseudo
     CDS             complement(3451..4891)
                     /locus_tag="SDA10_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            5284..5731
                     /locus_tag="SDA10_002407"
                     /pseudo
     CDS             5284..5731
                     /locus_tag="SDA10_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
BASE COUNT         1837 a          919 c         1088 g         1887 t
ORIGIN      
        1 acaaaagtat cttcgattaa tacttgctca agttatgtgc agctacgaaa aagtaggtac
       61 cgaggtactt tctttttcgc agcttttttt ggacttatga gaagtacatt cgctggaaga
      121 atacgatata aaacgagctt tttcactaaa gatattaata gagtgaaaga aacaagcttt
      181 tgttgtcagt tttttcactt ttctaaaaat gaaaataaaa aattctggaa gtggagaaga
      241 attttgcttg ctatactttg tcttgtagaa aagaggtaga caaaataatg caagctcaaa
      301 taagcgattt tccataacct tggtaggaat gacaaattaa ataggaaagg gaagtaataa
      361 tatgaataaa aaaaagttat ctctattcgg tttttttgcc ctgacagctt ccatggtttt
      421 gactgtttat gagtatccga cctttgcaac atcacaatta catttggttt tctttcttct
      481 tttgggaggt attttatggt ttctaccagt tgctttatgt gctgcagaaa tggcaacagt
      541 tgaaggatgg aaagatggtg ggattttcag ttgggttagt caaactttag gagaacgttt
      601 tggttttgca gctatttttt tccaatggtt tcaaattaca gtaggtttta ttacaatgat
      661 ctacttcata ctaggagcgc tgtcgtatgt aattaatttt ccagagctta atacagatcc
      721 atttatcaag tttattggtg tattagtcat tttctgggta ctgacttttt cacagttggg
      781 cgggaccaat cgtaccgcta aaattgctaa agcaggattt gtcattggaa ttgttattcc
      841 ttcgatcatt ctctttggtt tagcagcagc ctactttatc ggcggtaatc cgattcaaat
      901 ggctattaat gcgaaagctt ttattcctga cttttctcag ctttcaactt tagtagtatt
      961 tgtatctttt atattggctt atatgggcgt agaagcttca gcatcacata ttaatgagct
     1021 ggaaaatccc caaagaaact acccattggc aatgttttta ctagtgattt tggccatcgc
     1081 cttagatgca atcggcggtt tctcagtagc agcggttatt ccacaacatg acttgtctct
     1141 cagtgctggt gtaatccaaa catttgaaac gcttatttta cattttaaca gtcatttaca
     1201 ttggttggta aaagtgattg ctctcatgat tgcctgtggc gttatgggtg aagttagttc
     1261 ttgggtagtt ggtccatcac gagggatgtt tgcagctgct cagcgaggtc tgttaccagt
     1321 tttcttccgg aaaactaata aacaagacgt tccagttccg ttgattatga tacaaggaat
     1381 tatcgttact atttgggcag cagtcttaac ttttggtgga ggaggcaaca atttatcatt
     1441 cttagtagca atctccttaa ctgttgtcat ttatctcgta ggatatttac tatttttcct
     1501 aggttacttt gtattagttt ttaagaaaaa agacttaaaa cgaacttaca atgtaccagg
     1561 aaaaactgtt ggcaaaacga ttatcggtgg aattggtttt attttatcag tgttcgcatt
     1621 gcttatctct tttgtaccac cagcttcaat tgcagctagt gagcaccgaa cataccaaat
     1681 catactactt attagctttg tagtgactgt tcttttacct tttatcattt atgaagtaca
     1741 tgataaaagt gctcacacaa ctattgagga acctactcac gtgaaagctg aagacgttaa
     1801 tcccgctgtt tatccagctg ctagaggaga gcaccacgtt acaaaaaatg atgaacatat
     1861 tttaaaacat taggaggaat tcattatgtt atacggaaaa gataatcaag aagaaaaaaa
     1921 ctatttggaa ccaatttttg gctctgcaag tgaggatgtt gacttgccta aatataagtt
     1981 aaacaaagaa tccattgaac cacgaattgc ttatcaatta gtacaagacg agatgttgga
     2041 tgaaggaaat gcgcgattaa acttagctac tttttgtcaa acgtatatgg aacctgaagc
     2101 agtgaaattg atgacccaaa cgttagaaaa aaatgcaatt gataaatcag aatacccacg
     2161 aacaacggaa attgaaaacc gctgtgtaaa tatgattgct gatttatggc atgctccaaa
     2221 taatgaaaaa ttcatgggaa cttcaacgat cggctcttca gaagcctgca tgctgggtgg
     2281 tatggccatg aaatttgctt ggcgtaaacg tgctgaaaaa ttaggtcttg atattcaagc
     2341 aaaaaaacct aacctggtga tctcttctgg ttaccaagtt tgttgggaaa aattctgtgt
     2401 atattgggat gtggaactga gagaagtccc aatggatgaa aaacatatgt caattaatct
     2461 agatactgtc atggattatg tggatgagta cacaattggt attgtaggta ttatgggtat
     2521 tacttacact ggtcgttatg atgatatcaa gggtctgaat gatttagttg aagctcacaa
     2581 taaacaaact gactataaag tatacattca tgttgacgct gcatcgggtg gcttttatgc
     2641 accatttact gaacctgatc tagtttggga ttttcaattg aaaaatgtta tctcaattaa
     2701 ttcttcaggt cacaaatatg gtttggtata tccaggtgtg ggttgggtct tatggcgtga
     2761 ccaacaatac ttaccagaag aattagtatt taaagttagt tacttaggtg gagaaatgcc
     2821 aactatggct atcaacttct ctcatagtgc agcacaacta attgggcaat actacaactt
     2881 tgtacgctat ggctttgatg gttatcgtga tattcaccaa agaactcatg atgttgctgt
     2941 ttatttagcc aaagagatcg aaaaaactgg tatttttgaa atcattaatg atggatcaga
     3001 attgccagtt gtgtgctata agctgaaaga agatcccaat cgcgaatgga cactatatga
     3061 tttatctgat cgtctgttaa tgaagggatg gcaagtccca gcctacccat tacctaaaga
     3121 cttggatcaa ttaattattc aacgcttagt tgttcgagca gactttggaa tgaacatggc
     3181 tggtgattat gtacaagata tgaaccaagc aattgaagag ttgaataaag ctcatattgt
     3241 ttatcataaa aaacaggatg tgaaaacgta cggctttact cactaaaatt cttcagagaa
     3301 atataaaaga agttaattaa ctaataaaag gagttgtgat taccacacag ctccttaaat
     3361 ctatttgaat aaatatatga tattcatttg ttacactctc aatcttagct gttgttctat
     3421 tcatcattac tcttattgta agtagttcag tcaaatttgc ttgttcaatt gatcgataac
     3481 agtatgaatt gtttgaaaag taatcggata tataattgtt ttctcactac ctaagccttc
     3541 ttggtataaa gaactatcgc aaagtataat ccgctgagta caaaagaaag tgtcgtctac
     3601 tatttctcta agttgtttac aaatgatatt actaatagaa aagtgttctc ccaaaatgtc
     3661 ttctaactgt tgtcgatagt ataaatacct aaagtctgac ggagctacta tgaacacttc
     3721 aattggagat ttccgtttat gctcatttaa aagacttaaa ctaatggtta gtttacgaat
     3781 agaattctca ttccaacgca gaaataaatt gttttgtttt cgccattgtt caagaatttc
     3841 atgaacttct aagtacaaag cgcgttgctc ttcatttaac aagtaagtct tatcaggttg
     3901 aaatatctga atatcagccc aaagtgagcg gataaacggg agaagtgctt tatcaaaaag
     3961 tatctgattt tcttcagata tattcagaga taagataagt ttttgactga gctcaatcac
     4021 caaagggtta tctgctacat ggttttgata aacaacgttc aagtcttttt gtaacaattc
     4081 aaagttatcg catagataat ttctagagtt gaatagaaag tagatataaa ggcattcatc
     4141 ttcgtcatga cataaagaga aagaaagttg ctctaatcct ttctgtatta gctgaaacaa
     4201 gggagtatgt cgggcaactc ttttttcttc ctgactaaaa tatattgggt tagtcttaga
     4261 tctctgcaaa ccgatagcga tacctaataa aataaattgt ttttcaactg tagggtaact
     4321 tcttgaaaaa aaatgttttt ctacataatc aatcaattgt tgaccgcttt gaataattga
     4381 tttatttagc tggatcaact ctgtccgatt tgtctcatag attatcgtta gtaacaaact
     4441 tcgataatcc ttttcaggaa tttcaaaatg gccatcagca tttaagtact gcatttgttt
     4501 aaaaaaatta atcaactcat ttcgtaagtt gtaaactttg cttaacgaaa tgaaggtagt
     4561 atcagctata ttactccaat tgtgttcccc tgataaataa tgtcctagta cattgaaaaa
     4621 gtcagagcgc tgatagatga gttgagtgag tgccaacctc gaaaacgaag agtcgaatgc
     4681 gactgtaaag ctacttctag atttctccac tgaaataatt tgctgatgga gtatgtcggt
     4741 aatgcattcc aagtcgttag tgacagacag tgagtgggca aacgttcaat tgatctgcta
     4801 tttcgcgaat ctgaatggtc cctttttcgt gtaattgctc acaaatgtaa acttgacgaa
     4861 aaatactttt ctccagatag gtttccaaca tagtactcct cctagtcatt ttatacataa
     4921 ctattataac tctaccaatc ggaatagtcc tgtaatatat tgaatcaaaa atataaatta
     4981 taaaataatt agatggggta tttggtttaa gtatccctta tatagtaata aatttatagg
     5041 tgtgattctc gaattaatac ccgaaaatgt gagagaattt ttatggattt tttaggatac
     5101 aaattaatta tttttgcttt tatcctgatt accagtccag tactgtcggt atcccatttt
     5161 gcagcaaaat catgttgata aagtgacaag aagactgatg agatatatta tccaaattga
     5221 gaagataatt aatcggattc ttgagtatac cctacttttt aagaaagtag ggtatacaag
     5281 cgtatgaata aagacataac tttccctgtg atggacgttc ggcttgttcc aattaccaaa
     5341 atacaggcga acgattataa tcccaatcga gtagcgtctc cggaaatgaa cttattggag
     5401 ctttctatca ttgaagatgg ctttactcaa ccattagttt gttattacaa ggaaaaagaa
     5461 gagaagtata tcttggttga tgtgcttcat aggtatcgtg ttgctaagga aagactcaac
     5521 ttaaaagttg taccggtaac agttattgat aaacccatat aagaacgtat ggcttcaaca
     5581 attcgacaca atcgtgcaag agggacccat gaaataagag gaatggaaat tgtcatccaa
     5641 agattagtaa aaaatggatg gtcagacaag agaatatcca aagaattagg catggacatt
     5701 gaagaaattt ttcgctttaa gcaagtgtct g
//
LOCUS       DARDHA010000158         5681 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000158 DARDHA010000000
VERSION     DARDHA010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.161"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(388..891)
                     /locus_tag="SDA10_002408"
     CDS             complement(388..891)
                     /locus_tag="SDA10_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEG4241285.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1250..2275
                     /locus_tag="SDA10_002409"
     CDS             1250..2275
                     /locus_tag="SDA10_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG4241286.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2598..3476
                     /locus_tag="SDA10_002410"
     CDS             2598..3476
                     /locus_tag="SDA10_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HEG4241287.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            3952..4068
                     /locus_tag="SDA10_002411"
     CDS             3952..4068
                     /locus_tag="SDA10_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEG4241288.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            4996..5334
                     /locus_tag="SDA10_002412"
     CDS             4996..5334
                     /locus_tag="SDA10_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241289.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5428..5658
                     /locus_tag="SDA10_002413"
     CDS             5428..5658
                     /locus_tag="SDA10_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241290.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         1934 a          853 c         1057 g         1837 t
ORIGIN      
        1 caggcagaat atactggcag aatcaaggag aagggagcct aaataaggag aaaaaccata
       61 gaaaatataa agtaattact tataaaacaa gctttttata gaaaaataat atacctgagt
      121 agggtataaa ttaagacgga attcctttct agattgagat attgactagg aagaatgtta
      181 agattttaag cctttaagct aaaaaaaaac cctttttgtt aaataagggg aatatagcct
      241 gtcagtttca aactgcaagt cgtcctgctg caataatata gtagattaga gatgaaaata
      301 gattttttta aaccaatgac tgaataaaaa gaccgtcata gatacacatt ggaatgtgtc
      361 tattacggtc tttttttacg aacgatttta ttgaagacgg acagttcggg tcaaaaataa
      421 aaaagctacg atcagaaata agaataaaat gtatcccaaa ataatggcta gacggtctaa
      481 cttatttgtc gatagatgtt tgtttagaag ttgataggcc cataaaccta tcagtcctcc
      541 tgttgtatta gttatcaaat cagtgatgtc cgctacgcca ataccgaata ggaattgaat
      601 caattctgcc aagaagctta caaggaaaat aatccctaat tttttagaaa aagaaagtct
      661 cttgaaattg acattcaaaa gcaccccaaa aggaaaaaag acaatcaagt tataagtggt
      721 ctcacgtaat accgtcgtat tttcaaaaga aaaagtagaa aaagggacga gattgacatt
      781 gctgtaagaa taagtgagaa attgtggaag attaaatgat agcttgaata aaatcaacca
      841 taatagaaag aataaataaa tccctaataa aaattttgat acataccgca tagacatctc
      901 ctccatttaa aaagtatacc atcgaaaacc gctgaaaaat cataaaaaag tatgaagttt
      961 aaggaccaac gtagttatcg tacgaaaatt agaagctttt ttttgttggg aatccggttt
     1021 ctttaacaaa gctaagatag cgagttgctt ggaaaagttg ctatcttttt tctgtcaaaa
     1081 cgactcggaa tgttcgtttt ttgttctaat ttctccaagt tgtttacttt ttgatactaa
     1141 gtttatgaaa aatcaagaaa tagttgttat actaggtaat gctgacaaac gggattggca
     1201 tttcatcttg gttcagcttt ttttccaatt actaggaagg agggatagat tgttcaagac
     1261 ttattttcgt ttcaataaaa tacaagataa tccttttcgt atcatgaatc tgatgctact
     1321 tatctttatt gtattatggt taggttacat aaatcatgat tttttgattt caagtttttc
     1381 atcattaggg atctttactt ttttctatta tcaaaacata ccgatgaaat atttgctgaa
     1441 acgtatgttt ctgatcggtt gcggactgtt ggttgctttc gttttgggga tcttatcgac
     1501 ttatgtttta tggcttgaac catttgccgt agctttggtt gctttttcca gcagattcgt
     1561 gttgcgatta ttccatattt ctaaaccagg tggcttgttt tttgcgatgc tttctgccat
     1621 gggaacgagc atgcagctgc caatcgctca attaccgatc gtctcacttt atttttttat
     1681 gggtgtcgtt tttgccctga ttgcggcagt gattacaaaa ttactggatt cacggcctga
     1741 gcagacaatt gaaaaagcaa ctttaaaaga aagatttcac gaagagcctt tagtgatcat
     1801 tgattctgtt ttctacagcg ctgccttatt tctttccgtt tatgtcagtc acggcttgaa
     1861 tctgcataat ccttattggt tgaccttgtc atgtgcatcg attttgctag cagaaaactt
     1921 ggatgcgatg aagcatcgac aagtccaata tctgattgga tcgatgggtg ggttatgtgt
     1981 gtcggcattc ttgtcatttg tgccgtttac gcagttgcag accatttttc tgatcacttt
     2041 tctatatgga atcgcacaat ttctagttgc tagaaattat gcagttgcta atatttttct
     2101 taatccgatg gcgttgatgc tatctacact tattcgtgga gcatacttga tttctttgat
     2161 cgaatatcgt tttttaggga tcgtcatagg aagtttcatc ggtttaggtg ttgcgtgggt
     2221 aatgacggtt ggtttgcaac attatttaac ggttgttcgt caaaaattag aatagaagga
     2281 caaatatatg tgccaaaaga ggttatgaca agatttttgt cacaacctct aattcaaggt
     2341 gttcaaaagc cagattctca gcattaagtc aaattcacac aaaaatgtgg aaaggaactt
     2401 tcaaaaaaat tttatattta ttctatcaaa aaattttgtt tatgatggta actgaataaa
     2461 ctttcctgag atggtcaatg ctttccttga aagtcttaca gatattcttt cttcatagaa
     2521 agtttaagtt ttatagttag tgtacaataa aagtatgttt ataccatatg gtgtccggat
     2581 aatcagtcat ttaaacaatg aggttcaaag atatgaaaag gggaaatttg atgaagaaaa
     2641 taagcaatga aatccaaatt ctggcagaaa ctttaactct tcccaaagtc gttatagagc
     2701 aaatgattgt acaagcaaaa aacatccttg atgaggtgtt tgagcaatat agtcatgaac
     2761 taatggatcc taataaagca aaggcgactg caaagaaaat agacaaagtc tatgaagagg
     2821 aaccttttgc agcattagct atttatctat atgcagcttc tcaaagttgg aaaaaaattt
     2881 atcaaccatt gaagattcca agaactatct atttggcaac aatgaatgct tttactagat
     2941 ttattcatga acatttccaa gtaacaggaa gctatcgctt tgaccgtggt ttttggattt
     3001 ggcattacac tagtggcttg atctttcgga ttggtgaatt ggagtacgaa atgacatatt
     3061 ttccgaaagg gtacacggat ctaagattag aaggaaagca gtgcctttct cttcatattc
     3121 cttccgatgc agatttatct cagaataaga taagagatag ttacgaaagt gccattcgtt
     3181 tttttgaact ttattttcct gattaccatt accaagtgat gtacacagat acttggttac
     3241 tatcacccaa tcttactaaa tggttgaaaa aagagtcaaa aatctgtctt tttgcagccg
     3301 attatcgatt attatcagtt gatgaacagg acgatagtgg agtcccttgg atctttggac
     3361 gagtagatgc ccaaatacat gattatccag aaagtacgag tttacagcga caagcaaaag
     3421 agcaattgct tgcaggcgag cacattggct ctggcttagg tattcttact tcataagttt
     3481 agtacttcga aatagtttaa aagagcgtga gacaaaagta aagaacactt ttgtctcacg
     3541 ctctaaacac gtataaacgg cggaagcaga agcaactcct tcggaaataa gctgaaattc
     3601 acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat ttcttggagt taaacgcttc
     3661 tgtcccagcc tcttttttag tgttctatcg aacacacagt acgttcaata ggtaaactag
     3721 ctataacaat agatattaaa gttaacggac aagagggcct tttttaggta gctaaaacgt
     3781 aataatgaaa atttatttcc agagatagga gtcaatcagc tagtctgatc gactgtttta
     3841 acaagttgac tttcatttta acttttgata ccatttaaaa cacaaatcgt aattgtttcg
     3901 atttgtgttt tttagcatga ctacaatcaa agaagaaagt gggtcgtcca tatggaaaaa
     3961 acagatatct caagtgcata ccgccgcttg aaaagcccaa atatcaaaac acgaaaacgt
     4021 gcgctgaaga tcatcaaaga tgtgaaacgg aatagtggga aaaggtaaga agaaggaaac
     4081 ttaattaaaa aaatagccga aaaacgtttt tcggctattt tcaataatcg gtttttgtgt
     4141 aaggtttccg aaagaactgc tgttgatttt ccatttttaa catatgataa attggtagaa
     4201 tctttctttt caaattgata ccattcccta attccagtta tatagttaac tacagaacca
     4261 caaagcacgt acgtttataa aagtattatc tgtagttatt tttcaattcc ctttcaacct
     4321 cttattttga ccagtagtat tgatcacttt accttttaac acctttgttc gtttttgaaa
     4381 catttttagt caagaatgga aacgtaacta cataatcaaa ggttttgcga aatatttcat
     4441 gaaatatttc atgtagaagc gagcaatacg gttgcaaata accattatcg tttaataagc
     4501 agttacgaaa gctcccaata tctctaacgt tcagctcact ccatttcttt tcttcaaaaa
     4561 aatcagtgat tcgtatctct ttctatttgt cgaaaaagtt aactttaaaa agtaacgccg
     4621 aaaaaatatg caaaaaaata aaaaagcagt tactattgct agaataacat caaggaataa
     4681 taagttttgg ttttcagata atagtcgata agttgtgtat tggaatttta gatacagtaa
     4741 taaagtattt ttcatgaaat tttgtgagaa tcactattac aagtatcttt aataaaatta
     4801 atggttaagg aggaacttaa agtagaatgt aaatgaatag ttgtagtcag tcgattgtga
     4861 tactagactc aaagttgcag gaataagttt ttcaatttgt gattggaaga taaaaaatga
     4921 aaaagaaggg ctttttttta tactttgttg attgtagtgt ttattttccc tctgtttagc
     4981 caagtaacat tttctatgac tcaaccagtt tcaagtgagg aacatgataa cctttttcgt
     5041 gctacagaag agtttaagaa atatcaggaa aatattgttc cggagactcc attagctagt
     5101 gagccaatta ctgatattga tggaaaccaa ataggatggt atatacagta cgaagttatg
     5161 gtggtaaata atgactcaat agtaaaaaat acagtaggct tagagctaac accgattgaa
     5221 aaggtgagtt cattgttaac gtatatttat actgatgaaa gtgacgagac tgagatcatt
     5281 ttaacggatt actcaaaaat tgctactgat aaaaagtata tattgtggat gtagacacta
     5341 gtgaaatact ttatgatata gaaattgatg aaaatttact agatgtagca caagaaatcg
     5401 atcatcaaaa gcaggaagct attcgatatg ctggtagaaa agggaaaaga aaatatttat
     5461 tacgtgaatg tagcaaaagt tagagaagat gaaaatgagt ggaaagaatt caagagtcgc
     5521 tactcaatta actcgactcc gacatttaca gtttacagag aaggatcgat agaaaaaact
     5581 gttttttgga ctaaagaaag tggaatgtct ttagctgaag tagaagagtt tttggattat
     5641 gttagtatgc aacagtaagg gagttcttat tatgaaaaat t
//
LOCUS       DARDHA010000159         5609 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000159 DARDHA010000000
VERSION     DARDHA010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.163"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            202..573
                     /locus_tag="SDA10_002414"
     CDS             202..573
                     /locus_tag="SDA10_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4241291.1"
                     /translation="MTVMRLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPE
                     TASSIRTIELDDNTITFLKAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIV
                     GVHIIRIHGLRHSHASLLISQ"
     gene            complement(857..1942)
                     /gene="xerS"
                     /locus_tag="SDA10_002415"
     CDS             complement(857..1942)
                     /gene="xerS"
                     /locus_tag="SDA10_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEG4241292.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            2276..2509
                     /locus_tag="SDA10_002416"
     CDS             2276..2509
                     /locus_tag="SDA10_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HEG4241293.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            2506..2913
                     /locus_tag="SDA10_002417"
     CDS             2506..2913
                     /locus_tag="SDA10_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEG4241294.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(4050..4568)
                     /locus_tag="SDA10_002418"
     CDS             complement(4050..4568)
                     /locus_tag="SDA10_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEG4241295.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            4935..5183
                     /locus_tag="SDA10_002419"
     CDS             4935..5183
                     /locus_tag="SDA10_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241296.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         1867 a          909 c          892 g         1941 t
ORIGIN      
        1 gggatttttc agatgtcatt agatcgagca gttgttttgg gactacttga aaaaaatcct
       61 gcaaaagtta ttggaaatgt gaagaaaagg aaaactacag ttgatttttg gacaaaagaa
      121 gaatttacaa aagttatttc aactttttat attgatgatt tgtaccaaca ttttggtttt
      181 atttgtatgt ggttactttt tatgaccgtt atgcgtttgg gagaagccac tgcattaact
      241 tggaaagata ttgattttga aaataaaaac ttgcgtgtga ataaaacact ctattataag
      301 agagccaaaa cctatgagtt tgtagaaccc gaaacggctt ctagtattcg tacaatcgaa
      361 ctagacgaca atacaattac ctttttaaaa gcttggaagg agcgtcaagt ttcaatagga
      421 ggcatgtcgt ttgtactttc ttataatgga acacctactc agaaatatac aatccgtcat
      481 attatgagaa gacatgcaac gatcgttggt gttcatataa tccgcataca cggattaagg
      541 catagtcatg cttctttgct tatatcacaa taaaaagatg cacttcgaat taaagaaagg
      601 ttaaaacatg ctgatgtaca aacgacttta ggaacttatg gtcatttgta tctaaattca
      661 ttgagagaga taacaaatga tcccacagat tatatccaaa ctaatttttc taaaatcaaa
      721 ccgacaccta tttacgataa taaaaaataa atgataaaca aaagtaggat cacaccaaaa
      781 gaaacacact agaaaaacta taatacaggc ttttctagcg tgttgtttct tatttccgcc
      841 tatttataca ttatactcat aaagcattta atgcatcgat cgtagccgca ttgtctatat
      901 gggtatataa agttgttgta ttggtacttg aatgtcccaa ttgttgggca gttaatacca
      961 atgaatttgt ctgttggtag agtctagtag ctaatgtatg acgcagtttg tgagggctga
     1021 cttgaattct aaagcctttt gaatattttg ctaccatccg ttcgattgca ataccggata
     1081 tacgcttggc ttctccttgg taaattgcta gaaataaagc tttttcgttt tctgttactt
     1141 tatatcgttc tttccgaata gctagataat tagctaaata ttccattgcg aatgctgcta
     1201 tattgacagc atctctttta tttccttttc tagttactac cacattattc ttatctaaag
     1261 ataggtcatc taaattaata tttataacct cggagagtct caatccagat cctaaaatca
     1321 gtgcattgat cgccaggtct ctttctacat ctcgttgata atagatcaaa gctcgtttac
     1381 tcaaagtatt tttatattca ttggcaatat aatctagata attttgtgta tccttatcta
     1441 gaaataattt tttctcaatg gcattggcac gtgcagctaa cgtagtttta tctgtgatgg
     1501 ttgctacttg atccatagga ttattgtcta agtatggttt tcctagagga ttattggaac
     1561 ttttcgaaag atatttaaat agaaccttta atgccgtgat atttctattg atagtagaag
     1621 tagatagacc taagttatta ttatctttga gcttagtttt tgttgtctct ttttttacat
     1681 tttcttttgt gcgacgtttc aaatgagatt tgtacaagtt taggtcatag gcagttaact
     1741 gtcttatcga ggtgatagaa gtatcaggaa cgatagactc actcagaagc cattcataaa
     1801 agagcttata attttttaga tattctaaag tggttcgagg tgattgatct ttatcctcac
     1861 gatcttgaac aaaatctttt atataatcgg gcatatgaag tatgattgta tctatttttt
     1921 gatatatttc tgtatttttc atgtcattca tcctttcaat aagtagggga gaagagtatc
     1981 tctataattg gactatacca attatagaat gttttgtcaa ttggtatgct ctaaaattta
     2041 ttaatttgtt tcaaaaaaag catataaaca ggtatacgtt taataattta aaataaatgg
     2101 tatacataaa tagggaagga aatagaaggg agcaatctaa attttttgaa ttttcttaat
     2161 taattatata gaaagaacaa tctttctata gagttagaca tgagagggac tatgcttgat
     2221 ttaaaatcaa caagcgtata tactgtatat acaaagaaaa aggagggaga agactatggc
     2281 aattaaagaa agaaaattta gggaagtggg gaactcagta gtattaacta tttcaaagga
     2341 gtttcttaaa gaaaatgggt taaatcccgg agatacggtt tttttagatg aagaaaaact
     2401 tcgagcggca attacaaaaa aacaagcctt atctgatgaa attgatttag caatatctca
     2461 agcattaact gaatatgata ctacttttag agagcttgtc gataaatgag tattcattac
     2521 ctagaagcca aagatattat aaaaatgaat gtaatccaaa taaaaaaata ctctcctaat
     2581 gaaccccttg gtgtaaagga tgcgaatgct ttagaaatgt gtgtagggca acttcgagcc
     2641 tcagcttttg gtgaagaggt ttatccttcg atttatgaaa aagcagctat cctacttata
     2701 caattgataa agaaacatcc ttttcacaat gcaaataaac gtactgcttt cttagctact
     2761 tttgtctttt tgaaaatcaa tggacagctt ttagctattg atcaaaaaga tgctgtaaat
     2821 ttagcggtat atattgctac gtatgataag gatttcgatc aactaaaata tgaagtgatc
     2881 aacactataa agacacatac gcagcccatt taaggagtta ctgtatgtgt ctttataggg
     2941 ttattagctg ctatagcgaa ataattgaag taaattacaa atagctataa ttcaaatcca
     3001 actatataga aaacgtaaat tttctatata gttggattaa gataacgact atttatttta
     3061 tcatattttt actataccct atacttttac ttggtatatt tatgaataat cgcaaattta
     3121 cccctatact actagtttgt aagttagatt aataaaaata ctaaagttat ttgttctttt
     3181 attaaataca cctacaactt ataaattgat ggtatttaga taacacgaaa gaataaaaat
     3241 aacagctgat agtaaagttt gttgacgatt gctactcaat cgaaagtgag ttagtgcgta
     3301 tgaacttgct tctgaaaact tatgaaatga aagacctttt acatcttgca gaaatcggta
     3361 ctgggattaa actaagcaat aaaaactaat gttcccctca ttctttgaac ttctactatt
     3421 tgatttattt attctgataa atgacgattt caccttcagg atcttcaaat cccgtataga
     3481 atttatttaa gatctcttca gtatcagtaa taggataggc gataggccac ataccctttg
     3541 atggttagaa taaactgctc aaaatctgta tcgtatgttg gctttagcat attaacaaag
     3601 gctatctcca aattataaac cataagcgtt gaatcgttct taacaatttc aattcttcct
     3661 aacccaaagt cagaagatat aattaccgga cttgccttac ccttcatgtg ggttgttgag
     3721 attccatatt taaagggaat tgtcggtact atcggcggtc tgaaaaacat cccaaaaata
     3781 aaaaaatctt tcccctgcta gtctcctaag tccctttgta gcggatatgg caccataatc
     3841 tcattttatc gaagtgagtc atggtttatc tgttggccaa ccaaaattga tacaatctaa
     3901 cgatgcaccc tctacggcat tctaggggag tgcacttgca ttcacccaat aaaaaccggc
     3961 agttagcaaa gaacttatgt agtcgtatgt tcttactgtt aacatacaag aggtctcctt
     4021 tccacaattg cttagcagta aacgggaatt tacaaagcta atctcttcca ttttcttatc
     4081 tttgttctaa aagtcccaaa tggagcaact gtatttacat gaataaattt atatacttcc
     4141 catacagccg ttttggttgc ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct
     4201 tcatcactca ttgaatcaat catcatgtta atatcatcaa tattacgatt gagaatttcc
     4261 tctaattctt tcaatggtag atgtgcatag gtatttgtaa accacttata taactctcct
     4321 aactgattcc atttaaattg atcagaaggc gttttaacag ataatccatt tctttcatca
     4381 ttttcccatt ttagaattaa attcgtccaa ccaacttgat atgctaaatt ttcagcaggt
     4441 gttctatcca cttcctcaac tcttttatcc ttcaattctt caggaatagt ttcaaattct
     4501 aaaatatatt tagtataagt cctatttatt tcttttttta attcttcttt atttttatat
     4561 gttctcatta tattacctcc ataaattcct aattgtctcc tttaattcat ctataatttc
     4621 atccacgata ttttaatccg ttctacaatg catatctgat atgcattgac tgtctaactc
     4681 atatgtgaat gatttataaa atatcccaac gtctcatcta aagaaatgtt gatcgtcagc
     4741 aaggagctaa aaaatacgaa tatatttctt ttgtagtaaa atcggactgg tttgcatatc
     4801 attttcttcg taaatatcaa aatcatgtta actactatca attactaata aaaactacca
     4861 ctttaagcgt taagaactgt taagtttagt taagtgagga tagttcacaa aatccattgt
     4921 taaacttcac gaaagtggta aaactcaaaa tgctttatat aggattacag catcgctctt
     4981 tcttcgattg cgcgttgggt aaaaaatatt atgaagtaaa aattgacgat gacacgttcc
     5041 ttacggctca tcagatgaaa cagctacaaa aacgaaatga tcaactcgaa gaaaatttaa
     5101 ttctaaaaaa gcgattgcca tattcacgcc tcgctcgacg aactattaaa ggtcaagatt
     5161 tacctgaaga tcatcctttt taggaaattg aagcttttta tgttgcacaa tgcatttata
     5221 atactacaat gatgttttca ccagatattc ttctagggtg cggtgtaatg aaacagatac
     5281 acttgcaaaa gaaaattcac cgcgaattta ctaaaattat gaataattat gttaatcttc
     5341 cgaaattttt ttccaaaatt aagggatcaa gctggtgtta tagattgttt aaggttggca
     5401 aaaaaataac ttctttttaa acaattatag aaaaccgata ctctgttaag ttggttgaca
     5461 cggtgtcgat ttttatgact ttcttattct gaaacttcac taacggacag tacctaaaaa
     5521 gtctatagta tggacgttta cgagtactat tttatcaaaa atgctgctca tttcaactta
     5581 tatgttttag tgaactaccc ccacttagc
//
LOCUS       DARDHA010000160         5594 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000160 DARDHA010000000
VERSION     DARDHA010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.164"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1048)
                     /locus_tag="SDA10_002420"
     CDS             complement(<1..1048)
                     /locus_tag="SDA10_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4241297.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNI"
     gene            complement(1260..2156)
                     /locus_tag="SDA10_002421"
     CDS             complement(1260..2156)
                     /locus_tag="SDA10_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG4241298.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(2149..2829)
                     /locus_tag="SDA10_002422"
     CDS             complement(2149..2829)
                     /locus_tag="SDA10_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4241299.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(2830..3612)
                     /locus_tag="SDA10_002423"
     CDS             complement(2830..3612)
                     /locus_tag="SDA10_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4241300.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(3605..4522)
                     /locus_tag="SDA10_002424"
     CDS             complement(3605..4522)
                     /locus_tag="SDA10_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4241301.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(4686..5009)
                     /locus_tag="SDA10_002425"
     CDS             complement(4686..5009)
                     /locus_tag="SDA10_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241302.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT         1652 a         1157 c          927 g         1858 t
ORIGIN      
        1 agatatttac tcccaatttg agtcttttaa ctgctgcatt gagatgtaat actagattat
       61 tgatcaattc ttcatcagga taaaattcat ttaatttaat tcttactaac tcttgtagct
      121 caacacccat atctaaatag gaaagcttcg tttgatctaa gtcgtgattc atagcatttt
      181 tttcaatcga agtaatgtga atgtcaacat aagcttgttc atctattggc acaataattg
      241 aaaatgcttt ttcaatcatt ttgattaaaa taagtgtatt ctttgagatt tcttcttcag
      301 aattattact acgtgataag aaattgcttg tttcaatcct ttctatcgca atagcaaggt
      361 ggatagctaa agtttgaaat tcataatctg taaatttcaa ttgtgtctta ttcatgtatc
      421 gattaagcaa ttcggctact tttttcaata tgtccttatt taagaaatta ttcgcttcac
      481 caatgaaatt gatattcaag tctaagagat ctgttttact gttagatgct gtaaagccac
      541 cccaataata gttcacaact tctgacatca accttctttt acttttttca tcagctttga
      601 tgcgcacgcc atttcgatct gaaactaagg acatcccatg cttgtttagt ctttttctaa
      661 catctttcat tgtgctttct accgtcgacc tgctcacgta taattcgtca gcaagatttt
      721 gaatctttag agaatcatta gtattcaata atttagcaaa cacataagtt agacggtcct
      781 ccggagagtc tgcgatattg attttatgac tggataataa atttcgaagt gtttcttttt
      841 ttccaataag cgaaacgcct tttttaggtt gaaccaacat ttgtacatct ggcatttttt
      901 ggattatttc tttcaaaatt aaaatatctt tgctaattgt tttcgtagat acatataatt
      961 gttctgataa ttctttgtaa gttgtgtgct cattttcaat caaaaagtat agtattttca
     1021 tttgccgttc gctaaattcg atgcccatta tcttcacctc ctgaatacag cttaagtgta
     1081 caattattat ctctattttc atacagaaga aagttcttga ttaatgtaga agtttctaat
     1141 attcttgatt atttcaaatc aataaaaaag cctgcaatat ttatatcaca ggcttttttc
     1201 tctattaaat atatataaga taatacattt ttctactagt tctcactaat cttgtataat
     1261 taaaccctcc gcaacttgac ggagatggag aagatgcccc cgtctaatct agcggtcatc
     1321 tctcctccca ttccttccac caattttttt gacaggtaaa gcccgattcc gctagattcc
     1381 gtatttgtgc ggctggtatt ttcggtataa aaacgctgga agactttttc aacagaggtt
     1441 tgcgttcctg gtttgatagg attggccact gttaatcgaa tgaatggatc ctcttttgaa
     1501 aatgtcacct ttgcttgtcc atctgcataa cgcaataaat tctgtatgat attttgcagc
     1561 actcgcagca acatgagcgg atctgctaat acagttgtat ttatcgagtg ttcagcgaac
     1621 gctacctcga tatttttctc agaaaattgt tcataaaaag agaaaaatac ttcttccacc
     1681 tctttagaaa aagaaagcgt atggagatcc atttcttttt gcttcgtctc tattcgagca
     1741 agatcataaa actgatccgt catactgagt aatcttgcaa cagattgttt gatgatcgtt
     1801 aaatacttct cccgtttctc cgtatcctct acctgttcca gtaattgtac ataaccgtta
     1861 atagcagtca aaggagttct gagatcatga gaaaggccga cgatcgatag tctgatgttt
     1921 tcttccattt cttgaaatgc ttgtcctttt tgttcatttt catccaacca tgtattcagt
     1981 gcatctacta ggtccattag attcttttct cgaaattcga ctgaaagacg accaccatat
     2041 tcttgtcgtt cagaaagagt ggcgattttc ttagttaatc tcttgatttc cctttgcagg
     2101 aagaaaataa aacttcccat aaagataaca acgagaagca acagccattt aatcaaatct
     2161 aaaccccact ccccacaccg tatgaatata cacagcatgt gcctttgcta gcttccctcg
     2221 aagattggaa atatgcatat tcactgtttt ctcactatca aaatatggtt cttgccaaac
     2281 tgtttcataa agatttgctc gcgaaaatac ttttttcgga tgttctaaaa aaagcttcag
     2341 cagatcgaat tctcttcctg tcaaatggac agccacacca ttcacattta catccctcga
     2401 ctctttgtcc agtaagattt ccttgtaata gatgcgttgc gacagatctt ttacttggac
     2461 agaagtctga tgacgaagtt ggatgccgat tcgtaacaaa agttcttcga tatcaaatgg
     2521 tttcgtcaca tagtcatccg ctcctgctgc caacaaggaa actttatctc tttgatcgct
     2581 ttttgctgaa agaacgatga tcggaacatt cgatgtctgt cggatttctg ctaacaatgc
     2641 ttcacctgaa agccctggaa gcatgagatc tagaaggatc aaatcgacag gatatgtgct
     2701 taacaataat tttccttccg tacctgaata agcatcaaga agtttgtatc tctgttcaag
     2761 aacctcctcc agtaaggaat gaacagcttc atcgtcttcg ataatcaaaa tagtttccag
     2821 caggaccacc tacttaatgt cagatttttg gaaaataaac attcctagag ctgatgatac
     2881 cacgattgtt actagactga ccagaatcat tttcggtgct tgcgaataag ttgcttgttg
     2941 cgcaaagtcc gtatacatga gccacgtttc cgtaaaccac tccgttagaa attccaagcc
     3001 tttgattttt gcgaaaacac cgggaatgaa ggccgttccc atgatataaa gaaaagaaag
     3061 aagcaaactg acactggtac ttctagtgat gaatgtcaaa gccactggaa cagataatgt
     3121 ccctaatgtc agcagaagca atacaggaaa tacttttaga acataatttc catagttttt
     3181 gattccctca acttcaccaa atccgaaagc aagtgtgtaa gcaagcatgg ctagtcccgt
     3241 gatcaaaacc acgccaagtg ttgtgatcaa aagcgtaacg agaagtttcg acacataata
     3301 agtcattcga ttccgtccta aagataacgt attgcgaatc gtaccgtttt gaaattcttc
     3361 cccaatataa aagcacgcaa atgctgcgaa agccagaaga atccatgcag ccattttttc
     3421 taccattaaa atccctgttg caccgttgat gactgtatcc gttgcttggt ttaattcatt
     3481 gccgtatttt gtatgcatat accattcgtt ccaagaataa aaaagcggaa gtacgaccat
     3541 cagtcctagt gagacccaca ttttacggct cttccatact tttatttttt cagcagttaa
     3601 tagattaaac atgtggattg cctccagtta attgtaagaa ataatcttcc agtttttgcc
     3661 caactaggtg aataccatcc acaggaactt ttgcttctac caatgcttga ttgatggctg
     3721 ctacttggtc aattccttca aaaagctgga taacacggct agattgtaca aaataatccc
     3781 tataaccaag agagtcgaga accgttactg cttgttccgt cgccgaagtg tcaattttta
     3841 taaattgacg agattcttgt gccaattctt ctttcgataa ttctttgatc aagtgtcctt
     3901 catgcaaaat accatagtga gtggcgatct gtgacaattc atctagcaag tggctagaaa
     3961 gcaagacggt gatgccccgt tcttttacca aacgttggat gatttctcgc atttctgcca
     4021 tccctgctgg atccatccca ttcgtcggtt catccaaaat caaaaattct ggattcgtga
     4081 tcagtgcatt tgcaatagct aaacgctggc gcatacctaa agagaaattc tttgctttct
     4141 ttttccctgt attttccaat cgcatcattt tcagcaggtc ttcgatttct ctatccgaca
     4201 caccgccgtt cgctgcttgg atacgtaggt tatctcttgc agttaattct ggataaagag
     4261 caggggtttc aatcgattga cccatacgtc gtcttgctgc ctgcaaatcc gtcgttccaa
     4321 ataattcgat cgaaccttca tcaatcgaga tcaaacccat aatagttctc ataaaggtag
     4381 acttacccgc tccattttcc ccaatcaatc catagatcat tccacgtttg atttcgattg
     4441 atactttatc taatgctttg gcagcaccgt attttttggt tataccagtt gctttcaaga
     4501 ccgtttcatt tccaaaatcc atacttatcc ttctttctta ttgatgagtg aagtatagtt
     4561 gacgggtata aagaaagcag ccagaaatgt ataaagaaag tgtaaatttt ccaattgaat
     4621 gtcaaaaaaa acaatcggac aatacgtgtc tttcctatta tcaggcagtt tgttatacgc
     4681 ttgtatcagt ttcctgattc tttcttttcg accaaagtcg tttctctgta ttcctcttca
     4741 ctgaaaattt ttgcttcttt cagatcaagc ttgagtaatt ttaccagacc ggttataagg
     4801 ataactatag caagactccc tcccagaaaa attagactgt ctcgcagacg tggttgcgtt
     4861 gtcttccaat caaagagcgc accaaacagt gctaatccaa gtggactggc caccatggta
     4921 atcgttgtta tcgttgacat aagtcgtcct tgtatatgcg ttggaacttc tcgctgaagc
     4981 cagaccatat aaaaagcaaa agaaaacatt aatccttaaa aacggactta tgaaaaataa
     5041 tacatgccgt ttgggattta caattagcta ccaaataaga ggttatgaca aaagactttt
     5101 cacaatccct tcttccgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca
     5161 atatttatca aacatgagaa gcaatttttg taaattcctt cttatttctt gaagctgacc
     5221 acttctgtca caacctcaga agcggataaa taatattgtt tgatgatact ttatcttttt
     5281 cccgtttgtt tatagatcga atgatattat agaccaagaa tcctacctat taacatagct
     5341 aaaaacacaa aaaaggcact ttccaaattg tggaaagtgc cagaaaagtt aggattaagg
     5401 taactgcgct cagcattgct tcgcccagta cgattctcgt aactcgtgaa ctcgaacgat
     5461 aatcgcaatt aacgttttga gaactgtgaa gctttacggg ctttcttaag acctggtttt
     5521 ttcttcgtat cattgaataa gtttgaaaaa ccttgattta taaaggtttt cccgtaaata
     5581 tattttatta tatt
//
LOCUS       DARDHA010000161         5565 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000161 DARDHA010000000
VERSION     DARDHA010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.165"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            502..1059
                     /locus_tag="SDA10_002426"
                     /pseudo
     CDS             502..1059
                     /locus_tag="SDA10_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1117..3156
                     /locus_tag="SDA10_002427"
     CDS             1117..3156
                     /locus_tag="SDA10_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEG4241303.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3265..4416)
                     /locus_tag="SDA10_002428"
     CDS             complement(3265..4416)
                     /locus_tag="SDA10_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEG4241304.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4570..5100)
                     /locus_tag="SDA10_002429"
     CDS             complement(4570..5100)
                     /locus_tag="SDA10_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEG4241305.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5199..5549)
                     /locus_tag="SDA10_002430"
     CDS             complement(5199..5549)
                     /locus_tag="SDA10_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEG4241306.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         2008 a         1027 c         1043 g         1487 t
ORIGIN      
        1 cacataaaac taaaaaatta tcaaagaatc ctggtgcaac tgaaagaatt tgcaacggga
       61 ttctttcaac ttatttgtta tttttttcta aaaaatatta aagattgtta aatttcacta
      121 tttcatttca aattccattg cctaatcatt aggaaaagtc tatcatgaac tcgtaaatag
      181 aatatagtaa taaccttgtt aggtgaggct cctatacaaa cataggctgt tgcccagaaa
      241 cgtcgaaagt cgccaatggg taaaacagaa actgttggat taaggctttt tccaatagct
      301 aagaatgttt tctttacgtt gtatagtgct aaaactcgga cgatgagatg atgctaggtc
      361 agaattcttg ttcgaaaagg tttctgacct tttctttata tttttgtgac tgacttattt
      421 gatgagacaa actcgctcct cacctgacaa gtggattgct gtgtgaggag gagatgcggt
      481 gaaaaaagat cgattggtgg aggtcgctcg gattcacttc tggttgcaca atacaaccag
      541 aaaacacaac aagcaaaact gatctctatc atgagagatt catatgtcga tattccaggt
      601 tacggaatga ataagataaa tgcagcttac tcttatgggg gagttgattt attaaatcaa
      661 acattaaagg aaaatttcaa atttgaagct ccctattatg caagtatcac atttcaagat
      721 tttatcgatt gcgttaatga actgtttcct ggtggagtca agatcgatgc agaaaaatct
      781 ctagatttag atggcgtata tataaacaaa ggagagcaag tgatggatgg taatacttta
      841 ctccagtatg ctcgatttcg ggaagacaaa gaaggagact tcgggaggat cagaaggcag
      901 cagcaagtga tcaaagctat ctctcagcag ctgaaagacg taacttcaat attcaagcta
      961 cccaaagcgg ttgggaaatt acttggtagt atccaaacga accttccaga aagtgtgctt
     1021 ctggattgtg gtatggattt cctcaaagac atatgttaga ataaacaggt ataaatagtg
     1081 aaataaagga atgacaagca agagaaggag gaaaaaatga aaagaagtga caagcacggc
     1141 aaaaatcgaa caggcgctta tattgccggc gcagtgatct taatagcagc tgcgggtggc
     1201 ggttattttt actaccagca ctatcaagaa acccaagcag tagaagctgg agaaaagaca
     1261 gttgagcaat ttgtccaagc tttgaacaag ggagattata acaaagctgc agaaatgaca
     1321 tcaaaaaagg cggcaaataa aagtgcatta tctgaaaaag agatcttaga taaataccag
     1381 aatatatacg gtgctgctga tgtcaaagga cttcagatat cgaatctaaa agtagataaa
     1441 aaagacgatt ctacttacag tttttcatat aaagcaaaga tgaatacctc attaggtgaa
     1501 ttgaaagatc tttcttataa aggaacatta gacagaaatg atggtcaaac cacgatcaac
     1561 tggcaaccca acttggtttt tccagaaatg gaaggaaatg acaaagtaag cctgaccaca
     1621 caagaagcag caagagggaa tattatagat cgaaatggtg aaccattagc aacaacaggc
     1681 aaactaaaac aattaggagt cgttccaagt aaacttggag atggaggcga aaaaacagcc
     1741 aatatcaaag cgattgcttc ctccttcgac ttaacagaag atgctatcaa tcaggcgatt
     1801 tcacaaagct gggtacaacc cgattacttt gtcccattga aaatcattga tggagcaacg
     1861 ccagaacttc cagctggagc taccattcaa gaagtagacg gcagatatta tcctttgggt
     1921 gaagcagctg ctcagctgat tggttacgtg ggagatatca cagcagaaga tattgataag
     1981 aatccagaat taagcagtaa tggaaaaatc ggtcgctcag gtttggagat ggcttttgat
     2041 aaggatcttc gtgggacaac aggtgggaaa ttaagcatta cagatgcaga cggtgtagag
     2101 aaaaaagttc tgatcgaaca tgaagttcaa aatggcaaag atatcaaatt gacaatcgat
     2161 gcgaaggcac aaaaaatagc ttttgacagt ctaggaggaa aagctgcatc aactgttgcg
     2221 acaacgccaa aaaccggtga tcttcttgcg cttgctagct ctccaagcta tgatccaaac
     2281 aaaatgacaa acgggatctc acaagaagat tacaaagctt atgaagaaaa tcctgaacat
     2341 ccattcatca gccgatttgc gacaggttat gctcctggat ctacgtttaa aatgattaca
     2401 gcagcaatcg gtctcgacaa cggcactatc gatccaaatg aagtgttgac gatcaacggg
     2461 cttaaatggc aaaaagacag ttcttgggga tcttttaaaa caacgcgtgt cagtagtgat
     2521 gtatcacaag tagacttaaa aactgctttg atctattccg ataatatata tgcggcacaa
     2581 gaaacgttga aaatgggtga gaaaaaattt cgtacaggct tagataaatt catttttggt
     2641 gaagaccttg atttgccaat tagtatgaat ccagcacaaa tttctaatga agatagcttt
     2701 aactcagata tcttgctagc tgatactgga tatggacagg gcgaacttct aattactcct
     2761 atccagcaag cagcaatgta ttctgttttt accaacaatg gcacacttgt ctatcctaaa
     2821 ttgattgcag ataaagagac aaaagataag aagaatgtca tcagcgaaac agcagtacaa
     2881 acgatcgtgc cagatctgag agaagttgtg caagatgtaa atggtacagc acattctctt
     2941 tctgctttag ggattccgtt ggcagcgaaa actggtacag cggaaatcaa agaaaaacag
     3001 gatgtaaagg ggcaagagaa cagtttcttg tttgctttca atcttgataa ccaaggttat
     3061 atgatggtca gcatgttgga aaataaagaa gatgatgatt cagcaaccaa acgagcatcg
     3121 gaactattgc aatacctcaa ccaaaattat caataaaaaa ttgcttttta agtacagtaa
     3181 aaggactgtg gcgttatcgt cacagtcctt ttttcaaata aacggttttc acgatgtctc
     3241 tttcacaact tctttttgat ttactcacac ttcataaact ttttttgtaa aatatttgag
     3301 aacaagtgga gaaataagtg tacttaataa aatcacgata actaaaggtg aatagtactg
     3361 tggttcgatc aattgacttt gctgtccaat ttgtaaaatg atcaaagcca tttctccacg
     3421 cgaaatcata ccagcaccga ccatcaatga actatgttga gaaaaaccgg caatctgagc
     3481 accaaaataa cctccaagca atttggtcaa aatcgctaca agcgtcaata tagaaataaa
     3541 gagtagttgt tcactaaagt cagaaaaatc aacttctaat cctacactga caaaaaatac
     3601 tggtataaat actgcatatc ctaaagcctc tacattgtgg tatacttctt ctttaacctt
     3661 tgtctgactg acagctatcc cagcaaaaaa agcaccaatg acagagctaa gcccaacaag
     3721 atccgctacg taagacattc ctaaacaaat aactagcgat acgatgataa tagaagaatt
     3781 tgcgtacact ttttctgcaa atgaaagtag gaaaggcgca atccatttga ctaataagaa
     3841 aataaaaaca aaataaatta gctgttcaat caaaacaagc ggtaaagaag cattgctaga
     3901 agcttccccc gttagaaaag ataggctgaa acttagaact aaaacgacta aaatgtcatc
     3961 taccacagaa gcacccaaaa ttgttgatcc ttcttttgta ttcactacgt ttagttcttt
     4021 taatacttct actgaaatac tgacagaagt tgccgcaaga ataatcccaa agaaaatcgc
     4081 ttcatttccc gaaacttgga atgctttgcc tgtcaaccag ccaaaaacaa ccgggaagat
     4141 gatcccaagc aaagcaacga acataccagg tcggaaatat tttttcaaaa gagataaatc
     4201 actttccaga ccagctaaaa acattaataa gatcacacca atctccgaaa agtcatggac
     4261 tagaatatca ggtttgaccc aattcaatcc agcttggcct agcaaaacac caacaagtaa
     4321 ttgtccaatc actgctggga tacctaatct ccgcgagata tgtgagccaa tcgttgttga
     4381 aatcaatatc aggcataaaa taccaataaa ttccattctc atcattcctt tgtataaaat
     4441 atagcccgaa cttagcataa catacattat tgaaaaaata ggaaaatata ctcacaaaaa
     4501 aaccaacaat ccaaaagatt tttttggatt gttggttttt atacggtcta ttttaataac
     4561 taaaaatgat taacgtatag tcatttttct tattctgatg aaattgataa tctcctggat
     4621 gcccatgaaa atcaaaataa agccgaatat ttggaataga agcatcaaac ttttgaatgg
     4681 attgaacaat aagacgaatc cgccaatcaa caacagaata ctactataaa cactccaact
     4741 tttagctttt gtattcatgc tgaagaacaa ctggaacaag ctgttgatga taacagctaa
     4801 tccaagaaga atcggcagga tagaaacgat tgcaggtgca aaagcataga cgatcagagc
     4861 taagatcagg aaaaaaagac cactgaacag accgatactc caagaacctg ttttcctttt
     4921 aaaacggtaa gcctcaaaaa gattcaacaa cccaagcaaa acaagatagg cggtgatcag
     4981 ataaccgata aaatgaaaaa cagcagaagg gtttagcaca atcgcaatcc cagaaataat
     5041 ataaacagca gctcttaata aggcgtattt ttgaatattt tgtttaatcg tctcaaacat
     5101 ataagaaaac ctcctttgac ttattttaac gtgaactagt cattttacca aattaaatgt
     5161 tacaaaatga gcgaaggaga agaatatctc tggaaaaatc aacttctgag gatcttttcc
     5221 atttttttgc cttttgctag ctcatcgatc attttatcta aataacgaat ttctttcatc
     5281 aagttatcct cgatgtcttc tactcgtaca ccacaaacaa cacctgtaat caaggaacgt
     5341 gaaggattgg ggacaggggc ttccgcaaaa aattgttcga aagtcacttg tttttgtagt
     5401 tgatattcta gttctgtttg ggaataaccc gtcagccaac aaatgatttc atctacttcc
     5461 tcttttatac gatcttttct ctccgctttt gctacataca taggataaac gcttgcaaaa
     5521 cttgtcgtaa aaatacgatt ttgcgccata atcagtcacc tttct
//
LOCUS       DARDHA010000162         5532 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000162 DARDHA010000000
VERSION     DARDHA010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.166"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(152..550)
                     /locus_tag="SDA10_002431"
     CDS             complement(152..550)
                     /locus_tag="SDA10_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEG4241307.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="SDA10_002432"
     CDS             complement(547..1320)
                     /locus_tag="SDA10_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEG4241308.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="SDA10_002433"
     CDS             complement(1317..2000)
                     /locus_tag="SDA10_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEG4241309.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="SDA10_002434"
     CDS             complement(2167..3054)
                     /locus_tag="SDA10_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG4241310.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="SDA10_002435"
     CDS             complement(3051..3920)
                     /locus_tag="SDA10_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG4241311.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="SDA10_002436"
     CDS             complement(4057..5343)
                     /locus_tag="SDA10_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG4241312.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
BASE COUNT         1715 a         1227 c          918 g         1672 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt tt
//
LOCUS       DARDHA010000163         5532 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000163 DARDHA010000000
VERSION     DARDHA010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.167"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..985)
                     /locus_tag="SDA10_002437"
     CDS             complement(<1..985)
                     /locus_tag="SDA10_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HEG4241313.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR"
                     /locus_tag="SDA10_002438"
     CDS             1137..1832
                     /gene="vanR"
                     /locus_tag="SDA10_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEG4241314.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="SDA10_002439"
     CDS             1810..2964
                     /locus_tag="SDA10_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEG4241315.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="SDA10_002440"
     CDS             3179..4147
                     /locus_tag="SDA10_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEG4241316.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="SDA10_002441"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="SDA10_002441"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEG4241317.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..>5532
                     /locus_tag="SDA10_002442"
     CDS             5177..>5532
                     /locus_tag="SDA10_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
                     /protein_id="HEG4241318.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRG"
BASE COUNT         1669 a         1078 c         1252 g         1533 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaactgt tttatgcaat gggctgcaca gccggaaaat aacctgacaa aggaaagtta
     5461 ttatcccaat attgaccgaa ctgagatgat ttcaaaagga tacgtggctt caaaatcaag
     5521 ccatagccgc gg
//
LOCUS       DARDHA010000164         5506 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000164 DARDHA010000000
VERSION     DARDHA010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.168"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(217..438)
                     /locus_tag="SDA10_002443"
     CDS             complement(217..438)
                     /locus_tag="SDA10_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241319.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(435..725)
                     /locus_tag="SDA10_002444"
     CDS             complement(435..725)
                     /locus_tag="SDA10_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241320.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(722..1906)
                     /locus_tag="SDA10_002445"
     CDS             complement(722..1906)
                     /locus_tag="SDA10_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG4241321.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(2088..3491)
                     /locus_tag="SDA10_002446"
     CDS             complement(2088..3491)
                     /locus_tag="SDA10_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG4241322.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(3513..4286)
                     /locus_tag="SDA10_002447"
     CDS             complement(3513..4286)
                     /locus_tag="SDA10_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241323.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(4296..4673)
                     /locus_tag="SDA10_002448"
     CDS             complement(4296..4673)
                     /locus_tag="SDA10_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG4241324.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(4694..5008)
                     /locus_tag="SDA10_002449"
     CDS             complement(4694..5008)
                     /locus_tag="SDA10_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG4241325.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            complement(5208..>5506)
                     /locus_tag="SDA10_002450"
     CDS             complement(5208..>5506)
                     /locus_tag="SDA10_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HEG4241326.1"
                     /translation="KMFKSRDGITLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNV
                     SDKGISSARKLLYVICSRAKNNLFLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
BASE COUNT         1624 a         1090 c          930 g         1862 t
ORIGIN      
        1 atcctttata ataagtaaaa tcgtcagtct catctaaagt tacctctgat attttcattt
       61 tagaaacaat ctcgttacct ccttcataat aatttgaaga gctctttctt tctaatgttt
      121 taattaaaag agatttaaat tgatcatttg taaacatatt cataaatcct ttcataacaa
      181 aatatatact tcctatttta tcatgaaaaa tatctgttaa gcaccaatga ttcggttaaa
      241 taagtttaat aacacatctt tcacgccagc tgcattgaac acaagtccga cggcgattag
      301 agcaataatc aagaagccaa tgagttttga aaattctcgt ttaaaaccta aaaacagtcc
      361 aatgacaaca atcgctaaca ataccaatga ctgtgcatta tccaagaacc agttgtacaa
      421 attctgccca aaattcatgt gattgtcctc ctttccttaa gatatttttc tcgataatag
      481 atatagcttt ttacacgcct taataattga aaaggggtta aatgctctcc atagacggtt
      541 actcgattgg tataattatt ataaaatctc accttgtaaa aatcataggt atagatggta
      601 tcaccagctt gccgttttcc ctcaataatg agagacgtat atttccctgt attttgtata
      661 tacgcttgaa actcaatgcc acactgaaac cctaacttct ttaattcttt tgttaaaagg
      721 ctcatgtaat caaatcctcc atggctagat gttgttgttc aattaatttc tcatgttttt
      781 ctgtcagttt ggcttcctga atcatggtgg agataatggt tgtaccgttg agaacatcta
      841 aaatgcttgc gatttttaat gtaggggcga cttgatgatg taaccagttc aatgttcgtt
      901 caaaagaata aggctctggc tgtgtggtta ggcgtaatgc tccacgattt ttaccgataa
      961 accattccca acgttcatta gttttccaat cactccgacg tttagtgtcg tctttatctg
     1021 caaagcggat atagcgattg ataatttcaa aggctgtttt ttccgcctgt tgatgtgcca
     1081 ataaatcttt catggcttgg gtggctcggt cattttttaa ccgaatttca aaccgattct
     1141 ttacttctgc gtcttctaag ggaacatcat ttttcacgta ttgctcataa tctttctcat
     1201 atatacagaa atagacttca ctttttaatg agccaatata aagtgtgttt cccatgtatg
     1261 gtttttcatc acggtgaacc aattcaccac tgcgataatt tttaaagctt cgaaagacgg
     1321 aaatgcattc ttcctgttta catttctttg ccaaatctgg aatattcaaa atccctgttt
     1381 tatcgttgat agctaaatcc aagcgtttga aaataccttt atgttccatg caatcttgaa
     1441 agaaatcata ccagctccga tgttgagcca atagaaagtt ttcaaattgc cgacagcctt
     1501 ttcctttcag ttctaataaa acacctttac ttacatcatg agagactagg acaacaatat
     1561 caccgaaacg atagtgttcg ggataagaat aatagccaaa gtcttcgtgt agcatataat
     1621 ccatattcaa acgtaaaacc tcaccgataa tttgagccac atcattggta gggaatcgaa
     1681 tccgtacata gtcgaataac atctccaatg ggttatcggg attgaagcgt tccaaagcgt
     1741 tcagtaaatc ttctttcacc tgttgagtag ggactgcctt gcctgtttca atatccgata
     1801 ggtaaggtct ggtaatatga gaagcaaccg ccagtttatt ttgagaaacg ccataagcca
     1861 gtcgcttttc tttcaattcc ttaatccaaa ttttttcgtt ttccattcgc aaacctccaa
     1921 tctaaaaaag atgtaaacta tgacccttga gtttacacct ttcatatttt ccgcaatcct
     1981 ttgtaccatc aacattggtt gatgagaatt gcctgttttc agttgtattt gtgcccccct
     2041 gttagataga gggggctaaa ggattggcgt ggctaacgcc acaccaatca aagcaagtct
     2101 gaacctgtac ctttcacttc gtgcggtaca ggctcagatt gctttttttg aatgactttt
     2161 cctatttctc ctaagaaatc gtaccttttt ggtacaaggg gtgtataaaa ctcactaatc
     2221 acactttttc ctgtatccac gtaacctctt cccttgatgg gttttaagaa aaattgtttt
     2281 tgtgtatcgc ttccgaacat cataccaaag cccatttcag acatacgacc caaagccaca
     2341 cggaagttaa agttgtcccg aatcccatca ctaaaatatt ttgcgtctgg acgttgacag
     2401 gcaagaatta aaaagaagcc tgcttgtcgt cctaacatta caatttgttt gagtttatga
     2461 ataacggtgg tactttcacg acctagcata tccatcattg ccacatattc atcaaaaata
     2521 agaaaattgg cagacaaacc aagataagca taattttctc ctgttttata gttagggtgt
     2581 tgtttcatgg tttcactgcg atcaatcata tcttcgtaaa attgattgat acatgccaac
     2641 atatcttctt tacggtaata gacattattc atgaccgccc ctaagtctgc caaatcagca
     2701 tttttagggt caagaatata aagctttgca tttgtatgca ggagagattc aatcaatgtg
     2761 agaataaaat aggattttcc actaccagtg cccccagcaa ttaacatgtg agggagagaa
     2821 tcgaactccc aatagaccga ttccatcaat ttgacacgtc catcttgagc taccacgtct
     2881 tcaatgccaa ttcgattgcc aatagtatca taaagtagga catactctac ataagaatct
     2941 ttcaacgttt tttccactaa ttcacaatac aatcctgttt ctagtttgtt ttctaggtgc
     3001 aaaagctgat cttgatattt ccctaaggtg atttctacat gaatatggat tagtccatct
     3061 ttcattcgat aatatatttt aggaaaatgg cggattcgtt gtttggtttt cttgctgggt
     3121 aaatctttaa agaatccctc tgattgagtt tcttcggctt cataccaatg attttctaaa
     3181 atcatacgag ctaacttttg acggtgctct aactgtttta tagaatctat acgatagtga
     3241 taataaagcc agctgataag tccgcataca aaaagagcag tgagaatact gctccaaaag
     3301 tatggcgtat gaagttgata gaaaaatgta tctaactgaa ctgcttgcca gttcaccgat
     3361 tgtaacgctc tccaatgaaa agggagtagg acgactagaa aaactaccag caatgaaatt
     3421 agtacaaaat gatagaccaa gtgcttatcg ttggctttga ttcgctctcc tctgtcatga
     3481 agcaatggca taaaaatcct cctttccacc tatcaaaata attgtgtatt gtagatacga
     3541 gcctgtttat caaaagttgg ttgagaaatg gcttcatatt tctgataagc gattcgcttt
     3601 tcaaagcgta aatttttttc tttcggataa taatgacgtc gatagagagt gtgtcctaac
     3661 agttttttcg cttcttttgt caacggatag agatagcgaa acatgcgtcc attgattttc
     3721 tcaatccctt tgtattcgca aaatgcctgg gaaagccaat gctttttctc tacctgttca
     3781 aagcgagcat tttcctctaa taaaaggcga gcacttcgag ggtgtatctt ttcccatgac
     3841 tgcttatcac gatagacact tgtcttgaaa tacccgcagt aaaagaagtt ggacgcttga
     3901 tagacatagc cacattttcc ctcaattcca tctgccagtg tatataaaaa atgacagtcc
     3961 gtatgttctt tcaaccactt aattagagct gaaagagctt gactgccaaa ataattggtt
     4021 tgattcattt ctggaagaaa acacatcttt ccaatttcta aataatctgt ggtttgaagg
     4081 gaactgtcgg gaaagagttt gcgtatggtt tgtaggggtt gcgtacccca gcctaattcc
     4141 acaacgccta ataatttttc ttctgaaaaa atccctagaa agtatttaca caatctaggg
     4201 agtatctttg aataatggta ttgccgaata aaagaaatgg cttcatcctt cggaatttct
     4261 ttcaaaatca atggtgtgtt tgtcataagc aacacctatt tttcactttc ggacgcttct
     4321 tttttatatt tctgatgagg ttttgtctgt ccttttaata caagatcgtc tgctttcacg
     4381 taccaatcaa cgtctgcccc tcgaaaagta gccgtcgcta cggtatctgc cacaggattg
     4441 attaattcca cttctgcgtt gtagtcaaat tctttcaaag gaacagaagc ggggatactg
     4501 acttgtatca tgcgtccttg tccttttgat tttaagtcat aggtacgttc tttaatttct
     4561 tcggaaaccg taccatcttc attctggata cgaacctcac gacgcaaggc ggaaaatttc
     4621 aatgtgccaa atgtctggtc tttttcaatc acaatacctt ctgctaatct catatttttt
     4681 cacctttcct ttcttatgct ttcaccatat cgtctgcgtg cataatgtaa ttggtaaatc
     4741 ctcgattgcc aattttataa ccttctgcgg taatatgtgg gttgatgagt ttgacttttt
     4801 cgtcggattc aaaatgtttc tcgccagctt cggagggaag aatgacaaca atatcatctg
     4861 ctcgttgaat cgttgagtat aaattgtaac tacgagagag aatggttggt ctgccattta
     4921 ttcgacgttg ttcaatgttc ccttcaccag catattctaa ttgtccaaat gttttttcca
     4981 tattgggaat gacatattta agttccatat ttttacctat cctttctgtt tttatgtttg
     5041 aatgaatctt atttgtgtga tggttttaaa ggggggagag cgaccttttg attcttgatt
     5101 ttttattttc ataagttcac ttcctttcaa aattgggtaa aaaaatagac gctccgtact
     5161 gagcgtccga ttcctactat atttaaaaca acgactacct aggtatttta aatattataa
     5221 tcaaattcta ttttatctaa taattgagtg ggataaagca agtctccttt actcgtaaaa
     5281 taacccacct ctgagaaaag aaataaatta ttttttgctc ttgaacaaat tacgtatagt
     5341 agttttctcg ctgaactaat tcctttatca gaaacattaa aaatttcatt ccaatgagga
     5401 attttcccgt ttagaagatt aatagcgata acagtatcat actcatttcc tttaacttta
     5461 tgtgctgtag taagtgtgat accatctcga gatttaaaca tcttct
//
LOCUS       DARDHA010000165         5499 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000165 DARDHA010000000
VERSION     DARDHA010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.169"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            13..234
                     /locus_tag="SDA10_002451"
     CDS             13..234
                     /locus_tag="SDA10_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016617603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241327.1"
                     /translation="MHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIKPFLEIGDE
                     PGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            297..719
                     /locus_tag="SDA10_002452"
     CDS             297..719
                     /locus_tag="SDA10_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241328.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(813..1130)
                     /locus_tag="SDA10_002453"
     CDS             complement(813..1130)
                     /locus_tag="SDA10_002453"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241329.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(1157..1363)
                     /locus_tag="SDA10_002454"
     CDS             complement(1157..1363)
                     /locus_tag="SDA10_002454"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241330.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(1344..1526)
                     /locus_tag="SDA10_002455"
     CDS             complement(1344..1526)
                     /locus_tag="SDA10_002455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241331.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(1540..1890)
                     /locus_tag="SDA10_002456"
     CDS             complement(1540..1890)
                     /locus_tag="SDA10_002456"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241332.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(1916..2218)
                     /locus_tag="SDA10_002457"
     CDS             complement(1916..2218)
                     /locus_tag="SDA10_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241333.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(2221..2454)
                     /locus_tag="SDA10_002458"
     CDS             complement(2221..2454)
                     /locus_tag="SDA10_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241334.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(3440..3762)
                     /locus_tag="SDA10_002459"
                     /pseudo
     CDS             complement(3440..3762)
                     /locus_tag="SDA10_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3885..5000)
                     /locus_tag="SDA10_002460"
     CDS             complement(3885..5000)
                     /locus_tag="SDA10_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEG4241335.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(5006..5404)
                     /locus_tag="SDA10_002461"
     CDS             complement(5006..5404)
                     /locus_tag="SDA10_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEG4241336.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         1593 a         1130 c          736 g         2040 t
ORIGIN      
        1 aatttaaatg aaatacatga ttgggctcaa aataatttac ttaatcgtaa acaagctgcc
       61 aaaatcactg gacaaaacta tgatgctttt tctagctctt tacgtagaaa taatatcaaa
      121 ccatttctag aaataggcga cgaacctggt ccttccatca ttcgcttata tctaagagaa
      181 gatattgaaa aatacgcaaa acagctcgaa gaaaaaagac agaaacgtca atgaggcgtt
      241 ttctgtcttg gatttacaag taaaacaata ataaacactt ttacaacgga ggttctatga
      301 ataagaaata cattgtttca gttggttcaa tcgtactgat tgtactcgct attattgctt
      361 tttctgtatc caacaaaaca gaaacacagc cacaaaatac ggttcaatcg actactacaa
      421 gtcaatctag cgatcattcc gtctatcctt cactaaatag tttaaaagaa acgcatggac
      481 aagatttaat tgatcttggt tcttctcaaa ttactactgt cttatttgaa aagaaaaata
      541 ttgcgtttat tactcctaaa gaaggaactg accttgaatt ttctatcaaa gtaacaaata
      601 ccaaagaaaa tttattctcc attcctgaca ttcaatatac ccctaacaag ttaaagattg
      661 gggatacgtt tggtttggct aaagtaaatg aacaatactt tgcttactca ctgaaataag
      721 tttctaatag caagaggaaa aaccctcttg ttatttttat cacataatca ttttataaaa
      781 acgcttcgaa aatatcaaca tttttgaaga aactattttt ctaatttctc aatttcttcc
      841 aagggatttt cgctaatgac ccatacaggc acagattgct ctcgaggctc cccttcttct
      901 aaccaccaac ctgcataatg aacttcttcc tcctcagcat taccatgata ccaaccaaca
      961 aaggattcat ctacaacgaa tgaaatttct tcttctggat caaataattc atttttccat
     1021 gcttcaattg cttgttcttt ggtgtacttt tgtttgttaa atacaccaca aatttcgtca
     1081 tctacataaa aaatttcaaa atcaaaatta ctcgttcctt ttactggcat cttaactttt
     1141 cctcctttga ctcacttcaa tagatatacc actcatgttc attccattgt ttactagtta
     1201 attggagttt tatcttaggg ctttgtgcac tctttccaaa aacttgagcg tcgttttttt
     1261 cttctaacca aaaaaggtaa tttcttacat tcggtactaa atgtttttcc tgcatttctt
     1321 catgaaaaac atataattct gcttcatttt tcaaccaatt cattattcat tccctcattc
     1381 acttcttcat accgccattc agatagtgag atttcttttt gtttctccac ttgtcctata
     1441 aaaatcgctg tattcaacaa gctgatcaca cttaataata aagcaatgaa actaatggtt
     1501 acacttactc tttcacattt tgtcattttg tttccttctt tactcgtttg ttatttcttg
     1561 taataggtct aataactcct tcaccgaaat ttgcaaatgg ttcgcaaaaa tttcaataaa
     1621 agcactcgca cacattacca gaccttcaac tgatatatca ctgcgacaat aaaattctgg
     1681 agaaacactg atttctgatt ctttcttaca attgaacgaa aaaactattt ttgaatcaaa
     1741 ttccggtaat tcttccacaa ctatgttttg ggattctacc aagaaaggga gtgcctgttc
     1801 taatgcttct tctggtgttt gagcagatag aataattgct tcttgttctt ttatttcatt
     1861 gttctcaaat actttataac tttttttcat tgttagttcc tctttcattc ctctctcaat
     1921 tagctaagcc gttactggta cgccattctc taaatttagt atcgctaatc cttaatgctc
     1981 gtcgaatatc aatcattcga taacctattg cacgaagttc tttatatctt ttagcagtta
     2041 gcgtctctaa cgtcatttca ggcaatgggt tcgaatcaaa aatagaattc gaaccgatta
     2101 tttgggccaa ttgttgtact tgtaacagct cttctgaact taattcagtg attgctcgat
     2161 tgcctatcat tgcatacatt ttctgtcgaa tatacttctt ttcttgttta gacaacattt
     2221 ctatccactc ctattttcat tatcatttgt tacctccact tcagtaaatc cacaaataaa
     2281 tgaattcata ttccaagtac cattcccttc aactacaact tttctcctgt cctctttcgg
     2341 atattcaaga atcagaccgt ttaccaatat gtttttcact actactaatt tgtttgtata
     2401 ccgtggaatt gctgttccga tatacttcac tttatttcct acgctaattg tcatttcctc
     2461 actcctattc ttttttatcc aaaacttttt cctgtcctat tcctgccaac gaatatactt
     2521 cactgcatgc ttaaacaaaa caaaatgttg ccctgtttta tttccgtctt ttacttgttc
     2581 cacatacagt tcatatttgg tcacaccttt taaaacgcca gatacttttt ttcatccatt
     2641 gtcacaatta gttttttcat caatcgattg atactgcact gctcattttt cttgttcata
     2701 tagtattcct ttctttttca aaactagcct cttttgttta ctagttttca tcaatcatca
     2761 acgatttaac agattttttc tttcgcaggg tttctagcgt cagcaacccc aaactctaaa
     2821 aaagtgcctt tgctcccttg aaaaacgagc ggacgattgt actcgtcatc atatgttttt
     2881 catactacct gatccacgca aataaccttt ttttgttctg tgccacaccc atccatacgt
     2941 atcgcttgag attcaccata cttttttagg tggtttatcc ttcctaagtc cctgttagac
     3001 acgctgatta acttagaaag tcctgtttgt atccaatttt caaagatctc ccaaaagaaa
     3061 taagtaggtt tacgtgtatc gcttctgttc aaagtacctg tttgtacttg agtatttcta
     3121 ctcccctcgt tcaagggatt ccttccacca gctagctttt cctttcatca tcaaatgttc
     3181 ctgaaaagca tttacgtttc gtgtccgtgt gcttacttat tcttttttcg atagcgatta
     3241 tccgctcgct actcgctatt ttatactttg tttgttagct caaataaaaa ctaacaaaca
     3301 aaaaaggcta gtttctttcc gttttatttt tttaactgtc gataaatagt tgttagttta
     3361 attccaagtt ctaaaaccaa tacgcccaac aaaaccacag gaaaaacaat tggggcaaac
     3421 aagtaaaatc ggatagtttt catgtcttcc ctctttcttt ttggtgtcgc tgtggtcgtt
     3481 cttgccgttc aatacttcga tacatttttt attcattatt tgctcgtttt gttttcctct
     3541 ggactgcttt tcttgctttt tgaatacttt atacgctttt cgtgcctgaa ttaataggtc
     3601 ttgtcggcgt ttcattcgtg agaaaacaat atttgaaacc atcaaatcct ggttcgaaca
     3661 accacgattc gcaataattt tcccatcaaa aaaaatagtt ttcacttgag aaaaaaacag
     3721 ttggaaatct tgttttgttg ctaatttttt tgtcaaaatc actgtgcttt tccctgttga
     3781 atctacaatg attcttcgaa caatccgatt cgataacttc caacgttgcc gattaaacaa
     3841 tgttacttca caacaaatat gacgtaagtg attaggggtc attttcatgt gtccaattcc
     3901 tccaaccaat tatgcatcga aattttgggc aattgtgaca gcccataaat ctgcgtttcc
     3961 aggtcttcat aactacaaga acgcttggct tgaagtaatg aatattctgc ttgatctgat
     4021 agttttgttg ccattggatc tagattcaat tctcttacac aacattccca aaaattcaat
     4081 agcgctctaa aataaaatcc ttttcgattc tgaattgctt ttccttcatg acgagcaatt
     4141 ttttcatttt gaatacaccg ttgatacgtt ttgcttaaat cttctgcaaa tgtttcccct
     4201 aaaattcgat actcgcttcc atcagataaa ccatgtgttt gattatactt tcgttgccgc
     4261 ttttctataa ttcgtttact gtcgtatatc atgtttagct gctctttcgc ttcacgccat
     4321 tcagctgaac tgcgaacaat ccatgtcaaa acatctttcg gaacaaacgt actttcgctg
     4381 taatgagcaa taaactcttt ttgccaatca tcagccattt caacaggact agtcgtatcg
     4441 atttttgtat ctagattttc tgtatctaga ttgttagaag aattattaaa taattgtgca
     4501 gaattttcac atggattttt ttcgtcaaaa tcatcgaaaa tcgctaaatt atcaacagat
     4561 tcctcctctt cttttccttg tgcagaatct acactggttg agtcaacagg acctaatgaa
     4621 tacacatcat tttctgtcgt atctaattct cctagataca accgagcttt gtttcttttt
     4681 cctgtttgtt gatcaaatcc catatcctct atttctagta gctcatattc tttcaattct
     4741 tttttgattg tattaacaga acgtctgcaa caattcaatg cattggctaa ttcttgttcc
     4801 gtaaattcaa aataaacaaa accttgacta tctacttgat ttcgttgaat ggctaaatct
     4861 aagcgtgacc gaaaaaccat atacgcgatt tttgctttat cagacaatga gcgatatttt
     4921 tcacttgtaa taaatacctt tggaaattga tagtataatt cattgtattt acgagttgcg
     4981 gtaaatcttt ttgttgccat cgttattaca cctcatcttg tccatatatt aagaaagaat
     5041 tttggtgttg gtagacacga acatcttcca ttgtagcaat caattctttt agataaattc
     5101 gaccgaattc aattgttagt aagtcgtaat cttctaattc atttttgatt tcttcaattt
     5161 cttcttcaga cacattgagt aaaatggcta attcttgatt ggtataagaa atatagacat
     5221 gtttcttttc atcaatccgt ttttcaatta aggattcttc taagtactca ttcagtaagc
     5281 tataggcgta ttttgcttgt gcagtcatcg cataatattt tgacgatttt ataaagaata
     5341 gttttggcat atgaaaagtt gcttgtgtca gcacatctgt tgcgcgaaat ttgtttttta
     5401 ccatattttt tcctcctaga aaaacacgca aaaaaagctc tgtataaatc gaattacaca
     5461 gagtttcttc ttgttgatat ttaatttcta aaatacgtt
//
LOCUS       DARDHA010000166         5428 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000166 DARDHA010000000
VERSION     DARDHA010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.170"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            35..1120
                     /locus_tag="SDA10_002462"
     CDS             35..1120
                     /locus_tag="SDA10_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG4241337.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            1117..3009
                     /locus_tag="SDA10_002463"
     CDS             1117..3009
                     /locus_tag="SDA10_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG4241338.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            3016..3393
                     /locus_tag="SDA10_002464"
     CDS             3016..3393
                     /locus_tag="SDA10_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4241339.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            3547..4329
                     /locus_tag="SDA10_002465"
     CDS             3547..4329
                     /locus_tag="SDA10_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HEG4241340.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(4455..5186)
                     /gene="erm(A)"
                     /locus_tag="SDA10_002466"
     CDS             complement(4455..5186)
                     /gene="erm(A)"
                     /locus_tag="SDA10_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HEG4241341.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(5244..5303)
                     /locus_tag="SDA10_002467"
                     /pseudo
     CDS             complement(5244..5303)
                     /locus_tag="SDA10_002467"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
BASE COUNT         1959 a          748 c         1036 g         1685 t
ORIGIN      
        1 gtagtagtta aagtatttaa agaggtggga acatgtgaag gttcaaagga tagaagtgga
       61 gaataagccg tatccattgt atttattact agataaagaa taccagctaa tagaaccagt
      121 aatgaaattt attaaatact tagataatac tggtaagtct cctaatacca ttaaagcata
      181 ctgctatcat ttaaagttgc tgtacgagtt catggaacag agaggtgtta ttcttaatga
      241 tattaacttt gagttgttag cagacttcgt aggttggttg agatatcctt cagcatcaaa
      301 tgtaattgat cttcagtcaa aaaaagccat aagagaagaa acgacagtga atacaatttt
      361 aaatgtagtt atgagttttc ttgattattt aagtagatta ggagaattta aatcaattga
      421 tgtatttaaa caagcaaagg gaagaaattt caaaggattt ttacatcatg ttaataaggg
      481 tagataccaa aagaatgtct taaagttaag ggttaaaaag aaacagataa gaacattgag
      541 atcagaggaa gttaagcaaa ttattgatgc ttgtcatacg aaaagagata aattaatttt
      601 aatgcttatg tatgagggtg gtttaagaat cggtgaagtg ttatcgctta ggcttgaaga
      661 tattgtcact tgggacaatc aaatccattt aacacctaga gatgttaatg ttaatgaagc
      721 ttatattaaa ttaaggaagg aaagaacaat acatgtgagt aaagaactta tgtcacttta
      781 tacagattac ttgatatatg agtatagtga ggaattggag catgattatg tttttatttc
      841 cttaaaagaa ggctattttg ggaaaccact aaagtaccaa agtgttcttg atctagttag
      901 aagaatagtt aaaaggactg gaatagaatt tacatcacat atgcttcgcc acactcacgc
      961 aacgcagcta attagggaag gatgggatgt tgcgttcgtt caaaagagat taggtcacgc
     1021 acatgttcag acaacgttaa atacctatgt tcatctttca gatcaggata tgaaaaatga
     1081 gtttaataaa tacctcgaga gaaaggagca taagaaatga atgcttctag taaaaggaaa
     1141 attattagtc agagtgagat tagcaaaaaa atagctgtaa tgaatgaaga aatgcagggg
     1201 ttttgggcta ataatagttg ggatataaga aaatgtccac atccttctgc catagaatta
     1261 agtaagaatc ctgctttaag gaatcgttgg gttcgttttg aacgtgttaa aaatctgtgg
     1321 ttaagaacag aattgaaata tttttatttt taccatttaa acaatggaat atggaatgca
     1381 aaaactgtct ggattagaaa aggaacagta attaataaaa tgttagattt cttagattta
     1441 aagtatccta gcattacttc aattactgaa gttcctattg aaaaagcaat gacggagtat
     1501 agaacttatt tgacaaaacg gggtgttaga attaccacta ctaattataa gattactgct
     1561 aatcaagaaa aaacacctgt aaaagctaat tcctactatg ttactaatct aaaacaattt
     1621 atggagtttt atgagaactt ttattttgat ggagaggagt gggataaaga cgtttgggat
     1681 agacgtaact tacctttgcc agatgataag gttaacccaa cacaatatga atatacaatt
     1741 aactttaaag ggtttcggaa tacatatttt aaacaacttg taaaaagata ttgtaagttg
     1801 agattgaaca tggatagctt ttcctatgta agtgatattg cccaaagact taaagagttc
     1861 tttaattttc tggacatgaa atttaaacaa gttcagagag tacaccaatt aacgagagtg
     1921 gaaattgaag catatttaag tgaactaaac atgatgggaa taaaacctag tacaataact
     1981 gggaggatct ctatattgga aggactattt agtacccttc ttaggctaga atgggatgat
     2041 gttccttcca aaatattaat ttattctgag gactatccga aaataccaag agcaaaacca
     2101 cgctttatag atgaattcgt cctagagcaa ttgaacagtc atcttgataa attacccgaa
     2161 tatatagcta cgatgactat gattgttcaa gaatgtggaa tgaggataag tgaattgtgc
     2221 accttgaaaa aaggctgtct attagaggac aaagatggag atttcttttt aaagtattat
     2281 caatggaaaa tgaaaaagga gcatatagtt ccaatatcta aagaggtagc tttacttatt
     2341 aaagttcggg aagataaagt ttcagaggaa tttccagata gtgaatacct ctttccaaga
     2401 aaagatggat cgccattaaa acaagaaaca tttagaggtg agttaaataa attagcttat
     2461 gagcaaaata tagtggataa atcaggtgag atttatagat tccatgccca tgcctttcgc
     2521 catacagtag gaacaagaat gattaacaac gggatgcccc agcatattgt gcagaaattt
     2581 ttggggcatg aaagcccaga aatgacaagc agatacgctc atatctttga tgaaactcta
     2641 aaaaatgaat ttactaaatt tcaggaaaaa ctggttacca ataatggaga tgtgcttgat
     2701 ctagatgaag ataatgaagt cgatgatgta gagcttcaat ggttcaagaa aaatataaat
     2761 gcacaagtgc ttccaaatgg ttattgtaga ttgccagtag tagcaggtgg ttgtccacat
     2821 gcgaatgcat gcttagattg cactcacttc tgtaccagta agcaattctt accacagcac
     2881 gaagaacagt tagagcgtac agaagagtta ttagccatag ctaaggataa acaatggcaa
     2941 agacaagtag agactaatag ccgtgttaaa gagcgtttag aacaaatcat tggaagtttg
     3001 acggggtaat tatcaatgga taaacaagtt agaaatacaa cagaaattgt acgtttggcg
     3061 aagcagaaat caaaaaagac aagggaaaaa gtagacaaag cgatttctaa attttcgatt
     3121 gaaggtaaag ttattaattt taattcaata gcaaaggaag ctaatgtttc taaatcatgg
     3181 ctttataagg aacacgatat taggcaaaga atcgaatccc ttcgtgagcg tcaaataaca
     3241 gcaaatgtag tctcaaaacc caagaaaagt tctcgttcgg aggaaatcct tattaaaacc
     3301 ttaaaaagaa gagtaatgga attagaaaaa gaaaataaaa aattacagaa ccaaattcaa
     3361 aaattatatg gagatctgta taataaagaa taattattaa tctgtagaca aattgtgaaa
     3421 ggatgtactt aaacgctaac ggtcagcttt attgaacagt catttaggta tatgtccaat
     3481 ctagggtaag taaattgagt atcaatataa actttatatg aacataatca gttacgaggt
     3541 gaaatcatga gcaatttgat taacggaaaa ataccaaatc aagcgattca aacattaaaa
     3601 atcgtaaaag atttatttgg aagttcaata gttggagtat atctatttgg ttcagcagta
     3661 aatggtggtt tacgcattaa cagcgatgta gatgttctag tcgtcgtgaa tcatagttta
     3721 cctcaattaa ctcgaaaaaa actaacagaa agactaatga ctatatcagg aaagattgga
     3781 aatacggatt ctgttagacc acttgaagtt acggttataa ataggagtga agttgtccct
     3841 tggcaatatc ctccaaaaag agaatttata tacggtgagt ggctcagggg tgaatttgag
     3901 aatggacaaa ttcaggaacc aagctatgat cctgatttgg ctattgtttt agcacaagca
     3961 agaaagaata gtatttctct atttggtcct gattcttcaa gtatacttgt ctccgtacct
     4021 ttgacagata ttcgaagagc aattaaggat tctttgccag aactaattga ggggataaaa
     4081 ggtgatgagc gtaatgtaat tttaacccta gctcgaatgt ggcaaacagt gactactggt
     4141 gaaattacct cgaaagatgt cgctgcagaa tgggctatac ctcttttacc taaagagcat
     4201 gtaactttac tggatatagc tagaaaaggc tatcggggag agtgtgatga taagtgggaa
     4261 ggactatatt caaaggtgaa agcactcgtt aagtatatga aaaattctat agaaacttct
     4321 ctcaattagg ctaattttat tgcaataaca ggtgcttact tttaaaacta ctgatttatt
     4381 gataaatatt gaacaatttt tgggaagaat aaagcgtcct cttgtgaaat tagagaacgc
     4441 tttattactt taatttagtg aaacaatttg taactattga aaatagaaag aaattgttcc
     4501 ttcgatagtt tattaatatt agtgacattt gcatgcttca aagcctgtcg gaattggttt
     4561 ttagtgaaaa gaacacgata ttcacggttt acccacttat aaacaaaaga tcgatacttt
     4621 ttgtagtcct tctttgaaat caatggttga tgtcgttcaa gaacaatcaa tacagagtct
     4681 acacttggct taggatgaaa atatagtggt ggtacttttt tgagcatttt tatatccatc
     4741 tccaccatta atagtaaacc caaagctcgt tgcagatttt gcaatctttt cgcaaatccc
     4801 ttctcaacga taagatagct atatttagcc tgactttcaa aggtaattct tttgacaata
     4861 tccgtactga tgttataagg aatattacca tatatcttat agtttatatg ttttgggaag
     4921 gaaaatttta gaatatccgt ttgaatcact tttatattct cagaggggtt taccgcttct
     4981 ttagtcactt gacataagcc tccatcaatt tctatagcag taactgatcg actcattttg
     5041 actagctctt tggtaaaatg tccttttcct gatccgattt ctattacgtt gtcttgttta
     5101 ctgatattcg tgtgattcaa tatttctttt acatgctttt tagaagtaat aaaattttgc
     5161 gtgtctttag ggtttttctg gttcattata accttctcct tactggttat aatgaactga
     5221 cattaagcgt tcattataac caattaattt tgatttggtt gataacgaac tttattaata
     5281 acaaatatag aaaaagtacc catactttta tctcctttcc atttttttca ttatgaatga
     5341 gataaagtaa tttctactac tgcgatactg gtgcacataa ttgaaacctc cttatgtaac
     5401 tgaatatgat tgtatcatct tttcattt
//
LOCUS       DARDHA010000167         5427 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000167 DARDHA010000000
VERSION     DARDHA010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.171"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(190..648)
                     /locus_tag="SDA10_002468"
     CDS             complement(190..648)
                     /locus_tag="SDA10_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG4241342.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(661..1992)
                     /locus_tag="SDA10_002469"
     CDS             complement(661..1992)
                     /locus_tag="SDA10_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HEG4241343.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(2003..3445)
                     /locus_tag="SDA10_002470"
     CDS             complement(2003..3445)
                     /locus_tag="SDA10_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4241344.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3432..4196)
                     /locus_tag="SDA10_002471"
     CDS             complement(3432..4196)
                     /locus_tag="SDA10_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HEG4241345.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4300..5175)
                     /locus_tag="SDA10_002472"
     CDS             complement(4300..5175)
                     /locus_tag="SDA10_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG4241346.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         1680 a         1125 c          780 g         1842 t
ORIGIN      
        1 tctcttaaat aatcaaattt atttttaaga atcctgaaaa taagttttct aaatcatagg
       61 agcaacaaaa aaattcgata attaacaaaa atcaaaatga ttaaatctag tgattgaaac
      121 atatcatttc aaaaaaattt tgtaaattca cccataaata tcaccctatt taactttttt
      181 catcgtacac taaaattcaa aacaactatc tttaatcttt acttcttggt tttgttttaa
      241 taaatttcct tcaaaaatgc ctggagaaac tacaatgatc accgtatctt tttgtgcttg
      301 ctccaacttc ttcaaatcta taatagctaa cagtgtccct tcttttacct cctggttact
      361 ttcaacccgt atctgaaacg gacttccttc gagttcaact gtatctaaac ccatatggat
      421 cagtattttt ctgccagatt cactttcgat cgttatggca tgtcgggtag gaaatatatt
      481 atcgattatt cctttaactg gcgcatggat atatccatca tgattcgtta tagcgtatcc
      541 aactcctatc atttttgaag aaaagacctc gtcttttact gctgttaatg gaataatttg
      601 accactagct acagctttta gaatgtttct tgttttaaag aaatgcactt tttatcacct
      661 ctactttaat gctggccaat attttttgtt tactttgatc atttcatcta gtattttttt
      721 tgcgactctg gcatttggta cggtttgatt caatgtaaat gcttgtaaag ctttttgata
      781 agaaccttca aagtaagcat ctactagtaa cttctctact gcaacttgtg cctccatcaa
      841 tcctttatga aaatcactga ttggttttct tagtgtaatt ggctccacac cagttgcacc
      901 aacatatgcc ggtatttcaa caactgcatc tgaccgtaga ttcggaatcg ccccttgatt
      961 aggaacgatt agcataaagc gacgacgatc atcattcaac aaggaaatag ctatatcgac
     1021 gatatattgt ccatgctccc caaaattaaa gttcaatact tcttccacaa taccgcggcg
     1081 tattttatca gccatttctt tggtattttt ctctcgtcca tccatgacca tattagcacg
     1141 tgtatactct ttgtcagtat gctcaacgac catatcagga tataagtaat actctaagta
     1201 attattcggg atattacttg gaaaattttt cgtgatgact gacatcattt gaaaggtttt
     1261 ttgccaagtt ttatctccta tattaaaatc ggcgacttgc atttcttttg ttattagttt
     1321 ttcaataatc tctggcataa tttctcgttg caattcttta tcataaatcg atgtatacca
     1381 gccaaagtgg tttaaaccat aatacgtaac gatccaattc ttacgatcat aaccgtaatt
     1441 cactgcaatc gtttcttcaa ttgaaatagt catatcacag gcattaatca tttttacatt
     1501 cggatattta cgacgaacag cttcagaaac gatcgactct ggatttgtat aattcaaaat
     1561 ccatgctttg ggggcatagt cttgtatatg atcgacaatt tccaataaac ctttgattga
     1621 gcgcatccca taagcaaatc ctccgagtcc acacgtttct tgtcctacta atccatgttt
     1681 aagtggaatc ttttcgtcct tttctcgcat ctccaatcct ccaacacgaa tttgtgagaa
     1741 aacaaaatcg catcctgtaa aagcaagctt tggatcatcc gttgcacgaa tgcgaatatt
     1801 tgaaaaacct tttcttttca gcataaattt aacaatcaag tacatatcca tatttctttc
     1861 ttcatcaata tcatataaac gtatttcaga taatggcaac cgtttttcat tgttcaaaat
     1921 ggcttgaacg attcctggtg tatacgtact cccgcctcca gctatagtga taatctgttt
     1981 tttcatcatc atcttttcct ttctattctt ctaataattt attgaattct gccataacat
     2041 aagtcacttc taagccgatg ataatttgat aatttttttc agttggttta atgatcccgc
     2101 ttatccctaa ttgcttgatt cgctgttcct cgaccatggt gggatccact acctctaccc
     2161 gtaggcgtgt attacaacta tagacatcca ggatattggc ttttcctcca aacgcttgta
     2221 gcatttttgt tgccatatat tcataatttt tacttgctaa ccctaactct gtttcttcta
     2281 aggttacttc attgccaaac tctgccttag cttcacgtcc taatgtctga atgtttctct
     2341 tattgatcaa ataataaaaa gtaacgtaat acaaggcaaa aaagaaaatt ccgattggaa
     2401 gaatcaaaat agcattggta gcgattcgaa aattgatgag ataatcaaca atacatgctc
     2461 cgaatgtaaa accaattcgt atattgaata agtaacagac aatccctgct aaaccggcaa
     2521 aaagtgcatg gatcacgtac aacataggag aaagaaacat gaaactaaat tcaacaggct
     2581 cagtgatatt tgcaataaat gaagttcctg caccgcttcc catgattcct ttgaccatct
     2641 ctttattttc tttataagct gctttataga tagctagaca tatagctggt ataccaaaca
     2701 tcattactac aaaaaaaact gataaaaaca gtccagcctg aggatctccg ttgataaagc
     2761 gtgtcatctc tcctctgaaa acttccttgc taggagatgt atagcttcct aaatcgtaat
     2821 aaatatacgt attcaacaca tggtgcagtc caaaggggat cagcaaacga ttcaaaaaca
     2881 taaatatccc cacacctaat gctcccatat caacgagcag ttttgcaaaa ttatcaatcc
     2941 caatttggat gagcggccag ataaagccca aaaaaacagc tgcgattgtt tgtagaatca
     3001 tcaccatagt caatgggaat ttttcgcccg caaaataaga aaaaactact ggtaattttt
     3061 gttctttaaa gcgattatac gtccatgccg ctactaaacc ggaaataatt ccaccaaaaa
     3121 tccccatatt cacttctggg tccataattt ctaagccttt tttcaaggta gcaatcgcta
     3181 aaaaaccagt aagtgcaggt attcctttgt ccttattttt gacaaaaccg attgcaactc
     3241 ctatagcaaa taagatatcc aagtttccaa aaacggcatt tccagctgca ccaattactg
     3301 ggatgttcag catatcgtca gctgataaac gcatgatcaa tcccgctaat ggcatcgctg
     3361 caactggaag tagcatcgct cttccaagct tctgcacatt tgatttaaca ttcattttta
     3421 ttcctcctct attaaatatc ctcatatgtt actaactcaa tcaaaggatc atttttcact
     3481 ttttgagcaa atgctgaatg aattaaaaat tcgacttggt gggtacgagg atacacaaaa
     3541 cttgaaccat ttaacaattc ctgatccaaa tacgctgcgt gacacatgac ttcgatcgtt
     3601 ttatcttctc tcaatatcat gagtaaactt gccaaatatt cttccacact cgtattgatt
     3661 tgtcgttttt tttcatcagt ttctcctacg acatcgaaac aaggctcaaa ataatcgaca
     3721 tgacgaactt catcttttcg ttcaaagttg ctccgaaccg gcaaatcata tttttttgct
     3781 aatgtaacaa ctacctcctg attttcatcc aaagtatgag tatgatgatg actgtctaaa
     3841 tgagttggta tgatccccgc atgataaatt ttttgaatct gagcattcca ttcctgatac
     3901 agttgatctt tgtcaatgtt aaatggatgc tgataataag ataaggatct gaactctccg
     3961 ctttcgataa gagtatcgac agttttgagg agtggtttgc cacacgtcaa tgttaaatgg
     4021 actcctacac ctagcctagg catttccttt cttagtttca cagcatgctc gaaacccggc
     4081 atatttgcca tcaatgtagt tgatgtcaaa attcctcgct gataagcatc cataatccca
     4141 taattcacac catggctata gccaaaatca tccgaattaa aaatcacttt tcccatttca
     4201 ttctccttct tttttctact aaaataggcg caaccaaata atcatagtcc atttactatg
     4261 tgtcatttgg ttgcgcctat agatagttac gcccttattt taatagattc ttgctagatg
     4321 aattgccaaa tagatgattt cattttcttg aatggcaagt tgaaattgct gttcgatgat
     4381 atctttaatc tttagtgctg tacaatacgc tttgctatgt ttaaatgttt cagtaaaaaa
     4441 accaatattt tctactggcg tttcttcctg ataaatcaag cgtttaacca ggaatttgat
     4501 atgaaccatt aagcgatgat aagtcagtga ttctttatca taaacgtttg aataggattt
     4561 ttctagatgg gacaaaatcg tggtcgtcat ttctactatt tttaagctct ttaaactatt
     4621 aggctctcct agctcagcat taatgatatg catagtgatg aatcctactt catttgaccc
     4681 taactcggtt tgatatcgat gattgattcg ctcgaccact tctactgcta tatcatattc
     4741 atttggataa aggaatctca tttctggcaa cataattgct gtgacatctc tatcttctct
     4801 ttttcgttga atagcaaaat aaatatgatc tgtcaatgta atatagatat ttgaatctaa
     4861 tggagaggag atcctagctg ttatcagttc aatcacttct tccgcaaaat ccataaattc
     4921 gattggaatc tgcgagacta tctcactgaa tcgtttgatc ttttctttag aatctaatac
     4981 aaataatttg tcagcatcct caacatcaaa tggcttactc actttagcag aaaagccaat
     5041 ccctttcccc atcgcaattt tttctattcc atcgtcatcg atcaatacag cattatgatt
     5101 cagctttttc atcacataca ccgttaattc acctcctcta tatgataaaa caaaacaact
     5161 tgcagactcc ttcatacata tttttgtctc caaacaattt ttaaagacaa aaaacgacct
     5221 aagaaagaac cctttcttag gtcgtgccta attgaatagt agcaccctac ataatttaca
     5281 aaaatagttt aacacataat aaaagcgtta tcaatatctt ttttactcgt tttatgataa
     5341 tacttgtatc gaattgattt ggaggtaatt acataaaaac aaaaaaaata tagcaactct
     5401 ctataagaaa gttgctatat tgataat
//
LOCUS       DARDHA010000168         5411 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000168 DARDHA010000000
VERSION     DARDHA010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.172"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(59..2008)
                     /locus_tag="SDA10_002473"
     CDS             complement(59..2008)
                     /locus_tag="SDA10_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEG4241347.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2050..3108)
                     /locus_tag="SDA10_002474"
     CDS             complement(2050..3108)
                     /locus_tag="SDA10_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HEG4241348.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3227..3682)
                     /locus_tag="SDA10_002475"
     CDS             complement(3227..3682)
                     /locus_tag="SDA10_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241349.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4266..5069)
                     /locus_tag="SDA10_002476"
     CDS             complement(4266..5069)
                     /locus_tag="SDA10_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG4241350.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1569 a         1083 c          753 g         2006 t
ORIGIN      
        1 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
       61 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
      121 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
      181 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
      241 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
      301 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
      361 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
      421 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
      481 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
      541 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgctg tattcattag
      601 aattttcctt gtttgtaaca cgagatagtg agctatatcg gtttaacgaa tcgaacatct
      661 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
      721 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
      781 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
      841 aaggatcatg ccttacatga gtacctataa gtcgtctaaa acgcattgca tcatctttgg
      901 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
      961 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
     1021 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
     1081 ttgttatagt ataaactgct aacggttgtc cttttatgga ctttaattcc tcttgaagtt
     1141 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
     1201 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacag
     1261 caattgattt agccggttcg gacaaatcct cacgataaag tttgtttgct tgtacgattt
     1321 cttgaagtaa ttcatcagga atcctttgcg ataatctaaa agaagcctga ggatcatctt
     1381 taacagagga tgtatttagc cattgtctca aaatatcgac gacaacatcg ctttttagtt
     1441 cattcaattc taacggttgt agccgtctcc ctaaggcatc atctcttttg atatagtttt
     1501 catattcttt tcttgtagta gcggtaatca tttgaagtga ttcatttgct aacggttctt
     1561 ttagcgaatc acccccaatt ttattgccac tcccaaaaga gctaacaact ttatgaaact
     1621 cgtcaatgaa gaggacaaat tcattatctt tgtcctcagc ttttaaaatt cgctcatatt
     1681 ctttcaatct tggaattaat gtttctaaac gtgtttggag gacatcatct cccccaacac
     1741 gcaattgtcc gattgataat ttatatactt gtactttctt tccatcttgt cgctgtcttt
     1801 cagcccattc acttgcaagt gctgttttac ctgttccggc ttcacctaac aagatagctt
     1861 gcggtgtttc cggtctattc aagatcagtg acaactcatg gagttcgtca tcacgtccca
     1921 ctaacttatg ttttggtggt ttcattggtg tgagagcatg attaaattcg gtttgaatat
     1981 tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc acttcctcta aaataagaat
     2041 aatttacttc tagtcagtca aagttaggta cttaaatgtc gtcttcatgt ctccaaaaga
     2101 atctttgtta aagtcagcat ttttgtattg atcaatcaat ttttgtgtat cattgattgc
     2161 tttaatttcg gtctccattc gactgtctag ttgacgaata ctttcttgcg aatctttatc
     2221 gtcaggattt tgttgtaaac gttccagata ctcagctttt gtggcttcaa ttttcttgat
     2281 gttagtattt gaagcattta actgatcttc tagcttattt acaacattat tcttacttgt
     2341 aactgctaca acttttgaat aatcaatatc gccatctttg gtaagaaagc ttccttgata
     2401 gacattagtt gaatctgaat atggatttat tctgaaattg atattatcag gagtattaac
     2461 aggttttgct tttttcccaa aagaaattac tccttcatta ttaacatcat tcatagaata
     2521 ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc
     2581 agctgtatca tcaccagaaa ttagtgatac ttggtttgca ataaaaactt gataagctct
     2641 ttcctctaat ttacctttta ggaataagaa agcatcccca tcagtactta acatcccata
     2701 gctcatttca agcttcttag gacgatcttt ttcttttgtt gccatatata agttatagtt
     2761 ttttccttgc gtagataaca actttctagc gttttgggag taaccaagtt ttactactgt
     2821 tacatctcta ttttgatctg tccacacatc tgaaatagtg atttctgctc ctgatcgagc
     2881 aaactccaat gaagacccta taggtgttgt attgttcacg agataataat tatagtttcc
     2941 taattgataa atgtagctac ccaagcctgt taataaaaaa agcactccta gaaaagttgc
     3001 aagcataatg cgtaatccgt gacgtgttct cttatcctta tcaccaagca accctccaat
     3061 aatactgtat tgatctttgg tagatgttga attgacgttt ttttccatca gtttgtctgc
     3121 cccctttcat aaaagagcaa acaatcagac gttttttcgg taattctagg ttcgggattt
     3181 tgaatcattt cggtatattc ttccccatta atatttaaca caaaaattat cttcctttct
     3241 tcgttccttt cttttgctct ttattttcca aataatcaat gatttttgct attgtaccaa
     3301 gatagtaatt cccctcagct ttgttttttc ggattttgct aaccattgga gtaggtacaa
     3361 tctcttcatc tctcaattca ctgaaagatt ttttacctag atattttcca ataacagatt
     3421 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
     3481 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
     3541 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
     3601 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
     3661 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
     3721 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
     3781 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
     3841 tcataaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
     3901 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
     3961 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
     4021 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
     4081 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
     4141 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
     4201 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
     4261 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
     4321 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
     4381 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
     4441 gcctgctcta gtattctttt gtcaatattt gtcattctat aacttgaagg tatacttaat
     4501 gctcttctaa aatcttcaac tttgactacc caataaccat ttcgccactc tttatttctg
     4561 tattgcatga gatgacgata caattctttt gtgtagctac ttcttagttt tgtaaaatta
     4621 tcaagttcaa aacgagtaaa gtttttgcca attgagttta agataaattc aaattcctca
     4681 tttgccttaa ttgtaactgt ttccttctgt ttatctatcc ggaatttttg aaaaagccac
     4741 agctgttcat agacgtctga atcttcaaaa cggtaggtta aagtacctag tttgtcactc
     4801 aaagctttta acacagaata aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa
     4861 cgaaagaggt tgaaatcaaa agttacttct gctgttcctt gacgtttcat ttttgataaa
     4921 atagcccaaa acacatccat ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga
     4981 ccattgttat aacgcacaat tgcatttgtt tgttctgata ctaaatctac atcactatca
     5041 ataatctttt cggtaacttc ttgagacatt gattactctc caatcttctt tttgcgctcc
     5101 tttaatcatg ttaatctggt actaattaaa ggaaacaatt ttttctgctt gttacctaaa
     5161 ttaaatcaca actaaaggta acagtcaaca aagatttgtt actaatctaa ttttctattt
     5221 tttcgccttc caaatcaaga aaaccgaagg taacgtctta tcctagtgtg atacctcaaa
     5281 tgtccacttt tgttaccttt gctgtccaaa agtgttaccc ttactgtcca cttttgttac
     5341 ctttactgtc caaaagtgtt acctttatac ccctttaata cttactctcc caagagatta
     5401 agcctcccct a
//
LOCUS       DARDHA010000169         5248 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000169 DARDHA010000000
VERSION     DARDHA010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.173"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1038)
                     /locus_tag="SDA10_002477"
     CDS             complement(<1..1038)
                     /locus_tag="SDA10_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEG4241351.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKE"
     gene            complement(1082..1846)
                     /locus_tag="SDA10_002478"
     CDS             complement(1082..1846)
                     /locus_tag="SDA10_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEG4241352.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(1874..2572)
                     /locus_tag="SDA10_002479"
     CDS             complement(1874..2572)
                     /locus_tag="SDA10_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEG4241353.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(2584..3363)
                     /locus_tag="SDA10_002480"
     CDS             complement(2584..3363)
                     /locus_tag="SDA10_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEG4241354.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(3379..4323)
                     /locus_tag="SDA10_002481"
     CDS             complement(3379..4323)
                     /locus_tag="SDA10_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG4241355.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(4369..5148)
                     /locus_tag="SDA10_002482"
     CDS             complement(4369..5148)
                     /locus_tag="SDA10_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241356.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         1402 a         1112 c          911 g         1823 t
ORIGIN      
        1 ttctttcatc aactctgctt tgcgttcttc tgcatccata tacatactac ggaacttata
       61 catcttaaat gtcttaccat tttgaccaat acgttcttgt gcaaagaaaa tcggccctgg
      121 tgatttgatg taaataactg gtgctacgaa gataaaaata acacctgttg caatacatcc
      181 aataattcca gcaagaatgt caatcgttct ctttataaat gcttgcctca tcgtcataac
      241 attaatacta gatgtaataa cagtataatc accaactcgg ccaataaatt gttttccacg
      301 tgtactatca gacacttttg taagattcat atgtacagca agtcccattt ccgaacatct
      361 ggcgattaat tctttgggat atggaacact ttcgtcaaca ttgatgaata cttcatctac
      421 ccaattctgt aaaagatatt ccgctgcatt atcagcattt gcaactactg ggacacctcc
      481 gatggtagtt ccatacatat ctctgtctac tacaatcaca ccactaagtt tatacatgcc
      541 ataattcttc tctttaagat tacgaactac attttttgca atcttttgag atgttacaat
      601 aactaacgat acatcttcac cgtttttcat gcggtgatgc aacaaccctt tccaaagtat
      661 acgaattata tatgttaata atccatacac cacccccatt ataaataaag aaactctgga
      721 ataatcattt ccatcctgca ttgtaataag gtataaactg cacacaagta acagcataaa
      781 agattgcttt atagaagcta cagcctcttt ataatatcct ctttttaaaa catttttata
      841 tgcctcaaat aaaaatataa tcgtaagatt ctcaagttct ataaaaattg ccatgtggcg
      901 ataaatagga atcacatacg gattccagtc accctgtcgc attacatatg atatataaaa
      961 agcaagctgg acacaaaata agtctaataa aataaaatca atatgtttag accaaccact
     1021 tgagtttttt ctatacatct ctcactctct cctcatttcc tttttatcta cgaataaaga
     1081 actaaaataa accaaacttc ttctttttta cctcttcata gattggataa ttgatctcat
     1141 ccccgttgat cagatccttt gcgacttgtt tcatctgtac tactttttcc ttaccaaact
     1201 ccttggcaat ctgctcatag cactctttta agtaaaaggt gcgcttagag accccgtgtg
     1261 catcagaagc agccatctgc acgagattat gtttgaccat tgttttggct gttttctgga
     1321 tatctttgcc aaatacacca acataacttg gcgcagtaag ttgggccaaa catcccatat
     1381 ctaggaacgg aagcaaatga tttgggtctt ctcgaaaatg cgcgttgcgt tctggatgga
     1441 cgatcactgg tatctttcct agttctaaca actcaaaaaa aagccgttct gcatataacg
     1501 gcacttccag tgtcggaaat tcgatcagaa gatatgtatc atctaaatct gtaaataaaa
     1561 tttcatctcg gtggatgtct tcgatgagcg ttccggtaat acgtacttct tgtccttcca
     1621 ataacgtcaa aggcaactgt cttttctcta attcggcttg taacgaagcg actagagaaa
     1681 tgacttgtga tttttcattg ctgtattttc cattattgtg gtgtggtgtg cataaaatat
     1741 gcgtaatgcc ttgctgaatc gctttttcag ccattgcaat acttgcttct aagttttctg
     1801 ccccgtcatc gattcctggt aggatatggc aatgtagatc gatcatttct cttgtctcct
     1861 ctcttcttat tccttaattt ccgtagtaat aaccagcatc tttgctgtgt tctgctccgt
     1921 tgtatacgac acctaagact cgtgcttgta ccatatctaa cagttctttt gctttagtca
     1981 atgattcttt tcgtgacgta ttttcccgta cgaccaataa ggtgccatcc gtttttgaag
     2041 acatgatctg tgcatctgtt acggcaacga ctggcggcat gtcgaagatc actacatcaa
     2101 atagttgtct tgcttctgct aaaatctgat ccattcttgg cgaacttagt agttctgatg
     2161 gatttggtgg tttaggtcca cttggcagaa tcgacaaatt gtcgactggt gttcgttgga
     2221 tctcatctgc tacacttcct gatgaactaa gaacagtact taatccgctt gcgttgttca
     2281 acttgaacgt tttatagatg acgggcttac gtaagtctgc atcgactagc agcacccgtt
     2341 gaccagattt ggcaaaaaca acggcaatgt ttgctgcagt ggtcgatttc ccttctccag
     2401 gtcctgatga agtcacaaca atcgttttga tttgctgacc agctgcggag gcaaactgga
     2461 tattcgtccg aatcgtacga tattgttcag cgattggtga agagggattg gtgagtgtga
     2521 ccaaactgac tgcatgagtt tgggctgctt tttgtttctg tgttcgtgcc attttctttc
     2581 ctcctagact cttgagcggc ttctgcgtga tggcgctgtt ttatcgtttg tagcttttgg
     2641 ttgtgtctga gctggtcctt ttttacggat accagcattc aattctttcg ctgacatttc
     2701 tggaactgtt cctaagattg ggaatcctaa tgtatctgtc acatattttt catctttcac
     2761 tgtccgatcg agtaattcca ataagaatgc cagacctaca cctaccatca ttcctaacat
     2821 taagccagtc attaaattca atttattatt tggcgaaaca ggtgtcgtat tggcaactgc
     2881 acctgaaatg atcgagattt tatcaacatt catcacatct tttgcgttct cttggaaaac
     2941 ttgtgaagtt gtgttagcga tctgttgagc agccactgca ttcgtactta ttgcctgaat
     3001 cgagaacatt tgtgaatttt ctgcctgatt aactgagatg gcatctttga tttgttcagc
     3061 agtcattttc acattgtctt ctgtttctaa acgatctttg acttcattga gcactaaatc
     3121 actgacgatc atatctttat acgtattgat catttgcagg ttcgtattga catcattgac
     3181 atttgcagtg tctgattgtg gcagtgtcac gatcaattgt gcttgtgaac tgtattttgg
     3241 tgtcaagatg aaaaatgtta cgattcctga gatggccaag ccagcaaaca ttgagatgat
     3301 gattattacc aagtgtttgc gtaatgtttc aaaaatttct ttcaagctga ttgtctcttc
     3361 cattttttct ccactttcct atttattctc tagattcatt tgatctttta attctttttt
     3421 cacacgatca agttcttgat cactgacttt ttgataggag atattatctt gcatgaagcc
     3481 ttctccttgc agctgatctt gcttaatcgt accaaatgcc tcccgataat ctaaagcaat
     3541 ttttttcatt tcatcaaagt tcatgtctgt tttcatattc gactctagag cattcaaaac
     3601 ggattgatag ttgctgacac cgtttagact caatactttt ttcgctacgc cttctacgat
     3661 ctttctttgg cgcccttgtc taccgtaatc accatttggg tcttcataac gcatacgaga
     3721 ataagataat gcctgatccc cgttcaatgt aattttaccg attggaaagt catagccatc
     3781 ttgtgagaaa gtgaggtcat tattgacttc gataccacca actgcatcaa cgagttcctt
     3841 tagccctttc atattgatag agacatagtg atcgattggg atgtctacat atttttgtac
     3901 ggtatccatc gccatggcag ctccgccgaa tgcgtaagcg tggttgatct tgtcttttgt
     3961 gtcacgacct acgatgtcga catacgtatc acgaggaatg ctgacgatcg ttgtctgttt
     4021 gtcttcagga ctgactgtgg caaccatcat cgtatctgat cttccttgat cgacccgacc
     4081 taaatctcca gtatcaatcc ccatcaatag aacagagaaa gattcttgtt tgtccagatc
     4141 aactggtttc gttcgttctc cttgggttcg ttccactgat tcgtaagttt tctcaataga
     4201 atgtgagaga tccagatata attttgctcc tactcctaca attgctaata ctaatacgat
     4261 aagaactgct aaaataatca gaaatatttt tttccctctt gattttcttc ttcgtcttgc
     4321 catagtttcc tccgtttgct aaccttgctt gaatcaacgt tttaactgtc aatagcttat
     4381 attgctatag cccatttgct cagcaacagt ttgtgctttt gttgcgtatg ctgcgcttat
     4441 tttttcattc cggatctgct ctatttgact gagcaccgtc aaatattgat cgtaagtagc
     4501 ggtggcagtc acttgaccat cctttaacat cgtatcgaat aactgatcta gttgcgtgac
     4561 ttgcgcgatc tgaatatcag cgaatcctag atattgcata acaaccgttt tccaaggact
     4621 atctttaaaa aagctcatat tttcacgtac acgagcgaca tctgcacttg tcaccttttc
     4681 tttgatgatg acatcgtcta cagcttgctg ccagtttgat acatttttag taaataaatc
     4741 gttgacagct gtctggatct tcagttttga ttctgcacta tctaactgtg tcacgattgc
     4801 tttcttctca tcttggattg caacagcttc tttaggcaat tctgattctt tgatgtcgta
     4861 atcatctgct gaaagtttga cagaggatag cttatttgac aaacttgtga ttgtttcttc
     4921 tgttatttca gggtttagaa aaattttttc ttttgtacta tacagcttgt ctacttcttt
     4981 tgataaagaa gtcaaggtgt ctttttgatt ggtgatggca gattctgcat aattgatccg
     5041 ttgctgatgt tgccgctgat tattgatatg gtatccaaac aaacagatcg caatagcacc
     5101 tgcacctacc gccacagcag tcaatgactt gacgaatggc ttttccatag attcactcct
     5161 aagtattgat gatagataaa ttaagatata attcacttac ataaaaatga cagcttttta
     5221 tttaaaaaaa cataattaaa ctaatata
//
LOCUS       DARDHA010000170         5040 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000170 DARDHA010000000
VERSION     DARDHA010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.174"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            414..620
                     /locus_tag="SDA10_002483"
     CDS             414..620
                     /locus_tag="SDA10_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HEG4241357.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(822..1823)
                     /locus_tag="SDA10_002484"
     CDS             complement(822..1823)
                     /locus_tag="SDA10_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG4241358.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1828..3741)
                     /locus_tag="SDA10_002485"
     CDS             complement(1828..3741)
                     /locus_tag="SDA10_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEG4241359.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3909..4415)
                     /locus_tag="SDA10_002486"
     CDS             complement(3909..4415)
                     /locus_tag="SDA10_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG4241360.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4575..5015
                     /locus_tag="SDA10_002487"
     CDS             4575..5015
                     /locus_tag="SDA10_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEG4241361.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
BASE COUNT         1391 a         1008 c          769 g         1872 t
ORIGIN      
        1 ttatttttgt ttcttatttt ctaaaaaatt aaaaaaaatt ttggtgaatt aagtatttcc
       61 ttgataacac tggcttttgg cctcatttta ggttatcatt tgtaatttta tttcacagtt
      121 gatatgattg ttatgtaaaa taaaaaagtt taggatacat cgaatttttg gggaaattct
      181 tttgggggta tcctaaatct tttctattat attcccatat tttctattat tggtctagac
      241 gtatgatggt aaactttgta cctatctgat tgctgaatca ttaattgctt atcattagcc
      301 atctttgttt caagttcgct agatgatcat tttattcaca tcctaacaaa cttgtcacat
      361 ttatagattc ttaatctatt tatcatacaa ttataaaaaa ggaggtgctg cttatgaaag
      421 ctatccatat ttctttgctt tttttcgcta gtgcattgat tttatttgct attcatcttt
      481 gttcccagcc ttatatcaat aacaacggaa tattagtgga accagcattc ttttgcttac
      541 cactcggctt tttgtccctt cttgcctccg caggcttcgg atttgttgct ttttttaaac
      601 gatcaaaaca acaaatctga cttataaaat ttacgtaaaa gagatcgtga caataatcgt
      661 gtgatattcc caaatttcga ataaaaggtg ccccttgcca tctctaactc ctcagtcaca
      721 agttacaata ttcataaacc atgaaaagct tgtttattga aattgctcct tgtgacttgg
      781 agctaagtga cagattacaa cctcttttgc atcataagac tttactcctt gaaaaacatt
      841 actaactttt gatatgtttc ttgcgcttgt ttattgctga aatcaaactg atattcatgg
      901 ttcaaaacag catttttctc cgcataatct acttttgtga ctttcacccc cgctttctca
      961 aatacagaag ttacttcttc catttgtttc gtaaacgtat ttgtatttcc atccgttaag
     1021 aatgtgtttt gcgtccaacg atccgcatgt ctgaccaagg ctaaactttt gaccttctct
     1081 gattgttcaa agtccttatt atcaaagtaa gcccatccac atttttcata aagaaagcta
     1141 gagattttat cacttacttg agtaagtgca gaaggatcca acagaccaga caaagaaata
     1201 aacttcgtaa tctgcttgtt ctccaaagtc ggatcaagat gatcagcttt tttaatttct
     1261 tcattttgaa gtgctaacaa atattctcca ctaatttgtg cacctgccga atttccacca
     1321 atcgaaatat tcgaccaatc tgcttgatct tttatctctt ttttagctgc ttgaatggct
     1381 tcgttcaatt ggataactgg tgtaggatgt tttgcttccg gtgatcgttc ataatctata
     1441 tttacgatga tttgctgcgt gtctgaacat agatattcca aataatcacg acaatcattt
     1501 ttatcccctc ctacatatgc gcctccatgc atccagaaaa gaatcggtaa ttttttcttt
     1561 tccactttcg gtgtgtagat atccatatta ttatttttgt actttgaagg ataggatatt
     1621 tcttttttta tatcgatttg ttcgattttt tgctggaaat catctggtcg ggcatatact
     1681 tgttcttttt cacttgtgtc aaattgtttt ctaacaagca agctaatcgg ctttggtgtg
     1741 atttgattaa tcaaaaatac agtgactaaa ataactacga tgataactgg tactgcaatc
     1801 actattcgct tgaaccattt cattcattta ccctctcttt cttcgccaaa tccatgtgat
     1861 aatcgtgcta acagataaaa atccacctga ataaatgaga agcgaatgtt tcttttctcc
     1921 agttttcggc aacattcttt tttttgcatc tgtctttttt tgtactttca ctgtcatctg
     1981 aatcggttgc gtatcatcac ttgattgatc ctctcgaggt tcattctttc ccttattgtc
     2041 tgcattctct gtcggatctt gcttttttgg tggagctact gggatttttt tgtaatagaa
     2101 aataatagtt ggtatcgtct cattaaaaat cccttcagtc ggtccttcaa catttgctaa
     2161 ttcgtatcct tcgaatgtct tttgctctgt catataattt tctccataag ctccgtaaag
     2221 ctggatttcg tcctggtctt tgagtagact tttattgttt tcatccacat actttacgtt
     2281 tatctgatca tggaacacta ctttcaaaaa gtccgttaca tgtttcgtgt aggcagcatg
     2341 gtcaacttcc tgtgcctttc catgccctgc tccttctact aataaaagat ctttataccc
     2401 tcctgataac tcatataatt tttgcgcgtt agacacaggt actacatcat ctgctgttcc
     2461 atggataatc aatttaggca atgaatctga cattccaatc gtaggtagag cagcttttgt
     2521 atccaaatgc atctcctgat caaaatagta ttgtttcaaa aggcccataa ataaatcttc
     2581 atgctcttga tccccaatga tgtctaactg attttttatg gcatccggta gtgcgggggc
     2641 tactgtctgg ctgtacgtaa aacgtagttg ttcatcaatt gatgcaaatc cgcaattttc
     2701 aataattcct ttgacattga caggaatatc tttcgctaat acagacatca tcgtagctgc
     2761 tcccatcgaa ccaccataaa gtaaaatctt ttgagatggc cgctctcgaa cttcttgatt
     2821 tatccagtaa aggacgtcat ctgactcata ttgtccatag gtcacataat cgcctccgct
     2881 attaccagtc gctctggaat caacaaataa taaattatac ccggccttat aaaaatcatt
     2941 gtattcttct gtaacgatcc cgttattcca attccctcga aatccgccat ggatcatgac
     3001 tgttttatct gaaccttgat cgacataata tgcatataca tccatcattt tgccatcttg
     3061 tttgtttagt acagtttgat gaagttgttt tgtatttgga tagaagcctg tgttttctgt
     3121 aggcgttgtc ccatcatctg tacctccaag catcgacatc aacacactgt tttttgcaaa
     3181 agcgatatcg tacaatagat aagtagcaat cagatgtttg ataatctctt tatctacacc
     3241 ataatcatta acagcaagct cagtgactaa taactctatt aattttgact tgatgtcttc
     3301 tgttggtcca ttaagtattt ctgtcatctg tttaccgaag taattcaaat attctttacc
     3361 attgaaattt tcacctttac cgatatcatc attttctgtc ccattctctt tgtcacttac
     3421 tttagcagaa gaggattcgt cgtttgacat ttttgtagct gttgcttctt ctgtttgttg
     3481 gttatctgtg gtagcggtcg tttcttgatc tgtgctagta acagatgtct caaccgttgt
     3541 ttcagtattt gtttctgttt gtgaagtact cgatttcgtt tcatttccag catcagtagt
     3601 ttctgtggtt tctacctccc gagattccga ttcggaagaa atgctctctc catatactgt
     3661 ccattgcggg acgcttgtac ttacagcaaa taagatgacc aacgctccct ttaatacttg
     3721 ttttgttctc aagctattca ctccttttta tgtttctagg agaattatat gtaaacgcaa
     3781 acatttttta tacaaaaaat aaatggttta tcccataaaa atagaacagg attattttct
     3841 cattttacgt aataaagcag aatggataaa ccagtatcca ttctgctttt tttaaacgaa
     3901 gaaacaactt attgctccaa aaatgtcaga aatcgtttat cccaaatttt ctcgtaaaaa
     3961 gcgattgttt cctttgcttt cataaaagga ttatcataag ttgtcgttgc tcctctacac
     4021 atcataagct ggtatcctaa tccatgagca aacttcacag tcgtatctat acaatactct
     4081 gcttgtgccc cacataattc gatcgaatga cagtccaatt cagttaataa aacactcaaa
     4141 ttagtcttat aaaatgaatt cgcatgggtt tttctcacaa aataactgtg ctcaggaata
     4201 gataattccg acaatagctc ccatggtttt gtttgtggca ctaattgagc atcttcgtgt
     4261 tgaacgaaaa taactggttt cccaatcgtt tcgtatgttt tgattctttt attcactaat
     4321 gctgtcactg tttctaattc gtgtatcggc gaagaatcaa aacatacccc tttttgtaaa
     4381 tcgatcacta ctattgcgtc ggctgtgttt gacatacttg tgccttcttt cttttttctt
     4441 gatttaatag tatttcttat ctagctagtt ccattatacg tgacaacatt ttcttatttt
     4501 acacctctaa atggcacttt tcgactattt cttcatgtta ttatttcata aatacttagc
     4561 tattggagga atgtatgatg attaaattgt atacaggttc tggctgctcg tcttcaagac
     4621 gcgcaaaacg ttggcttatc gtccatgata taccgtttat cgaacagaac ttaattcgtc
     4681 aaccactaac caaaaatgaa tttatccact tactaaaatt gacaaacaac ggcgtgaccg
     4741 aaatgctctc tactcattct attagttata aaaatcttgt tcacagaata gatgaactca
     4801 gtttgagtga actatatatt gagattcgta catccccttc tttattgaaa aaaccgatta
     4861 tctacgacca atctgcaaaa cttttgactg gatttagcga agataaaatc aggatgttcc
     4921 tttctaaaac taaaagaaag caagagctca acgaactctt gctttctggt catcttgtcg
     4981 attttaaaga aaataatctt tttgcaatgt cttaattttg gcataaacaa tctgcttttc
//
LOCUS       DARDHA010000171         5015 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000171 DARDHA010000000
VERSION     DARDHA010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.175"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1192)
                     /gene="hflX"
                     /locus_tag="SDA10_002488"
     CDS             complement(<1..1192)
                     /gene="hflX"
                     /locus_tag="SDA10_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEG4241362.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGF"
     gene            complement(1194..2111)
                     /gene="miaA"
                     /locus_tag="SDA10_002489"
     CDS             complement(1194..2111)
                     /gene="miaA"
                     /locus_tag="SDA10_002489"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEG4241363.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2115..2852)
                     /locus_tag="SDA10_002490"
     CDS             complement(2115..2852)
                     /locus_tag="SDA10_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEG4241364.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2943..3470)
                     /locus_tag="SDA10_002491"
     CDS             complement(2943..3470)
                     /locus_tag="SDA10_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241365.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3650..4243
                     /gene="clpP"
                     /locus_tag="SDA10_002492"
     CDS             3650..4243
                     /gene="clpP"
                     /locus_tag="SDA10_002492"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEG4241366.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4347..4832)
                     /locus_tag="SDA10_002493"
     CDS             complement(4347..4832)
                     /locus_tag="SDA10_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEG4241367.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
BASE COUNT         1327 a         1127 c          858 g         1703 t
ORIGIN      
        1 caaacccttt aacttcatat tcgtttgttt cttccatgta cgcttcgtcc aaaaccattg
       61 tttgtcttct caactcgctt aatttctgtc cttcgttgct tggcacagaa agtgtataag
      121 gggtcattag ttccatcagc tgacgtttga ttgcttcgat caacgccatc ttaccttctt
      181 tcgtctgagt agaaatcaat acatttggga atagcgtagg cgtgaacatc gctgggtcga
      241 tctggtctgc cttattgtaa acagtcagta tcggcatttc tttcatattc agttcatcca
      301 tcaattttaa gacagtctgc tcctgtaaaa tacgatctgg agaactagca tcgactacat
      361 gtagcaaaag atccatattt tgactttctt ctaatgtaga atggaaagca tcgatcagtt
      421 gcgtaggcag gtcttggatg aacccgacgg tatccgttac agtaatctca aatccttcag
      481 caaaacgcca tctcttagtc aatggatcta acgttgcaaa caattgatct ttcgaataag
      541 tatccgcttg tgtcaataga ttcaagatag ttgacttccc ggcattcgtg tagccaataa
      601 gtccaatctg aaaaatctcg ctattttttc ttttttggcg attgcgctct cgatgagctt
      661 ctacttcttt caattctttc ttaacagcaa aaattttatt ccgtatatgt cggcggtctg
      721 tttctaactt tgtttcacct ggccctcttg ttccgatccc accgccaaga cgagataatg
      781 ttttcccttg ccctactaaa cgaggcagca ggtattctaa ctgagcaagt tcaacctgca
      841 gcttcccttc tttcgaacga gcacgcatcg caaaaatatc taagataagc tgcacgcgat
      901 caattaccgg aagaccaaca gcatcggtaa tcagttggct ctgtctcgga gttagctcat
      961 gattaaagat gatcaaatcc gcttcatgcg catccgcttg ttgaactaat tcttgtaatt
     1021 tccctttacc gataatcgtt tgccgatcca cttgtggtct cttttgtgta attgtgaata
     1081 cgacttctcc tgatgcggta ttcgtcagct gaactagctc ttccattgac tctgaaaagt
     1141 aacgctggtt tgcttcggtt tctaccccta cgatgatcac tttttcttcc attttattcc
     1201 gcctccagcc attttttgat tgtttgctca atctgacttt tttgatttgg ctcacttata
     1261 agatcaaacc actgtgcatc caaacggtta cgaaaccacg tcaactgacg tttcgcgtat
     1321 cttctcgaat gtagtttgac ttgttctgtt gcttcttcca atgtttcttt cccttcaaaa
     1381 taagggaaaa attctttata tccaatacct tgactggcct ggacttctcc aagctcatac
     1441 accttacgcg cttctgctac taaaccttct gctagcatca aatcgactct ttgattgatt
     1501 cgttcataaa gaatactacg cttcgtatct aatccaatca tgaggtaatc atacaattgc
     1561 tttggctctt ctttaggtgc cgtgatacta tgtcctgtcg tctctaaaac ttccagagct
     1621 cttatcactt tgcgttgatt gttccagtgg attttctcag cagctgccgg gtctttttct
     1681 ttcaactgat tccataatgc ttgggaacca attgtttcag ctaacgtttc atactttttt
     1741 ctaatctcag gattcgcttc ttctgtcgct cctaactgat aatcatatag aagggattgg
     1801 atatagagac ccgtaccacc agctatgata ggtaggtgcc ctcggttcgc aatctcggta
     1861 atcaattgtc gacctgaatg ctgaaaatca gcggctgagt aacgttgagt aggttcgatc
     1921 acatcaatca aatgatgcgg aacattattc atctcttctt ctttgatttt tgctgtaccg
     1981 atatccagat ttttatagat ttgcaatgaa tcaccactga tgatctctcc tccaaatagt
     2041 tgtgcgagtt ccacacttag cgctgtttta ccgactgctg tcggaccgac gatcactaat
     2101 actttttcca tattttacct acttttcgtt gattgtttcc accgaagagc tttctccgga
     2161 aaatcagtta ttattccatc tattttgagt tcttgacatt tggccatttc ttcttcatcg
     2221 ttaatcgtcc atgcacgaat tgcttttgga aaatcagctg cctggactgg atgttcgaac
     2281 aaccaagtat tttttggatg tactccttct atataagggg tttctgctgc tagttggatc
     2341 tttttatcag aagtactcat caataagtcc agctgtatct ccggttccag taatgcgatt
     2401 ttttccaaag aatccaaatg aaagctgcaa tatgcatgtt gaaaaggcca atctctactt
     2461 tttaatgttt cggctaattt ttcttcgata ccgggatatt catactgatc agttttaatt
     2521 tcaatcatta gcatccctcg gtaatttttt tggaggagca gtcctaacac ttctctcaat
     2581 tcaggaactt tagctgctgg aaattctttg ctgaatttgc cccctgcatt caattttttg
     2641 agttcttcca atgttttttc tcgaatcagc cctttaccgt tagtcgtccg gtccacttct
     2701 tcgtcgtgca taacgatcaa ataaccatct ttgcttagat ggacatctag ttcgatacca
     2761 tctgcaccta ctcgtacagc ttctgcaaat gcaggaagcg tattttctgg gtgggtgccg
     2821 ctgcttccgc gatgggcata gatttgtaac atctttcttc actccgttca gttctattgt
     2881 atcattaaga aaaaaagaaa gcattaccac gctctcatgt gcaacggcaa tgcttcaaaa
     2941 tatcagctcc aaacatcatc tttcgagaag aaaccaatcg gctggcgcgt catagtcttc
     3001 aactgagctt ttttttcatt gatcatttca gtcagactat ccgtaaaatt ctctaaaatc
     3061 acgcgtccgc ctttataagc atatccacca atcttcattg tttcatctgg agataaaatc
     3121 acttttcgat gggagatatt cttaaatagc tcgtctaaaa tataatttgg cagataaaaa
     3181 ctattttgcg ctgaaaatga ttggagatac gcaaaatcgt caatcgtgat ataacaatca
     3241 tcaaatgcat caaagctgac atattcttcc atcagcgttg tccgtttttg caagatctcg
     3301 ttgatctttt tatttaattg attcagcgtc tcgatttctt tgactttcaa ttccaattca
     3361 cgtctacgct gttctttatt ttccaagtga cgcatttgca gtatcatgcc gatatagaca
     3421 actacagcac ctgtcagcac tccgataagg ccagtaatac caactatcaa tctgttgttc
     3481 ctccttgttt tgtttctctg cttatcatac cataaagcgg gtttatgcga ctaatttgcc
     3541 taagtttttt atttgacctt ttttgaccaa aaatgtatac tacattttag gtattagaaa
     3601 ctaaattatg gtacactatt tctaaacgaa atcaaggagg aactctatta tgaatttaat
     3661 tcctacagtc attgaacaat cttcacgagg tgaaagagct tacgatatct actcacgttt
     3721 attgaaagac cgtatcatca tgttaagcgg ccaagtaaca gatgatcttg ctaattcgat
     3781 catcgcccaa ttgcttttct tagacgctca agattctgaa aaagatatct atctttatat
     3841 caattctcct ggtggttcag ttacagcagg tatggctatt tacgacacaa tgaactttgt
     3901 caaagcagat gtccaaacaa ttgttatggg tatggctgct tcaatgggaa gcttcttgct
     3961 gacagctggt acaaaaggta aacgatttgc tttaccaaat gctgagatca tgatccatca
     4021 acctcttggc ggcgctcaag ggcaagcaac tgaaatcgaa attgcggcac gccatatttt
     4081 gcaaacacgc gaacgcttaa acaaaatttt agctgaacgc actggacaac cgcttgaagt
     4141 gattgaaaaa gatacagatc gtgacaacta tatgacagct gaacaagcaa aagcatatgg
     4201 attgatcgat gaagtcatgg aaaacagttc ttcattaaat taatctgttt caaaggaagc
     4261 ttttttaagc tctcttatct aaaccaaata aaaaaggatg gtcaactcaa actgaccatc
     4321 cttttttatt ttatggatgc tactgcctat ctttgctcct tgaattttcc ttgcgcatgc
     4381 atccgaccta tctcgacata atgctggatg ttttctttgt ttttttgtat ttcttctttt
     4441 gtcaaaggac ggatcacttt agctgggcga ccaaaagcca agacattagg aggaataact
     4501 tttccttctg ttactaatga tccagcacca atcaaactat tctcaccaat aaccgcatga
     4561 ttcaatatcg tggtccccat cccgatcagc gccccttttt cgattgtaca gccatgtagc
     4621 atgcactgat gtcctaccgt cacattctcc gcaatatcta caggtgcatc atgatcgaca
     4681 tggatgattg taccatcttg gatattcgtt ctttgtccta tttttatcca attactgtcc
     4741 ccgcgtaaaa cagcttgata ccagatcgtc acatcttctg acaatgtcac atcaccaatc
     4801 actgtggcat ttgatgctat aaaccgttcc atatttattt ttctcctttc attttttgta
     4861 gaaactaatc ctattgaata aaggtatcat agcatatatt tatttcataa gaaaaaaaac
     4921 aggtaaaacc tgctcaggcc agatgtctat ttacagtatc tggtaactga cgttttacct
     4981 gtttttattg tacatctata ttttcttcta ttact
//
LOCUS       DARDHA010000172         4950 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000172 DARDHA010000000
VERSION     DARDHA010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.176"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            116..802
                     /locus_tag="SDA10_002494"
     CDS             116..802
                     /locus_tag="SDA10_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEG4241368.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            804..1748
                     /locus_tag="SDA10_002495"
     CDS             804..1748
                     /locus_tag="SDA10_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEG4241369.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            1947..2591
                     /gene="pcp"
                     /locus_tag="SDA10_002496"
     CDS             1947..2591
                     /gene="pcp"
                     /locus_tag="SDA10_002496"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEG4241370.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            2670..3947
                     /locus_tag="SDA10_002497"
     CDS             2670..3947
                     /locus_tag="SDA10_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241371.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(4131..4880)
                     /locus_tag="SDA10_002498"
     CDS             complement(4131..4880)
                     /locus_tag="SDA10_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEG4241372.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         1602 a          868 c         1059 g         1421 t
ORIGIN      
        1 aaaaaataac gaaaaatatt taaaaatagt gtctatttcg tgagaaaaca actatatttt
       61 ttatgtgttt tgctttataa ttaaaaaaat attgtcattt tatggaggaa aatatatgga
      121 aaattatctt agccttttag gcgtcttgat tgttattctc gggtttgcat ttaaattaga
      181 ctcgatattg atcgtaatgg ttgctttagt tgttacagct ttgactggcg gtcttggcat
      241 tcaaggaatg ttagaacttt taggtacaag ttttgtaaac aatcgcggga tggcgatttt
      301 tatcatcatt atgttggcaa caggaacgct tgaaagaaat ggtctgaaag aatcagcggc
      361 ttccttgatc aagcgattca agaaagtatc tgctggtatg attgtagata tttatgggat
      421 ctttcgaatg atctttgcgg cttttaacgt gagttttggt ggagttgctg gatttgttcg
      481 tccaatcatt ttaccgatgg cattggggac catcgaatca aaagggttag aagtccatcc
      541 taagcatgaa gaagaactaa aaggtatggc ttcagctatg gaaaatgtct gctggttctt
      601 tggccaagta ctattcatcg gaggagcagg ggcgttgcta gtccaatcaa cactgaaaga
      661 tctaggatat gacgtaacat tgggacagct tgctttagtg gaagttccag tggctatcac
      721 ggctttagtt gtggcaagtg tctacttcta tcttaaagaa aagaaattag cgaagaaata
      781 ttatccagga aaggggcaat aagatggact tttttacaaa tcctgatatt ttattaggcg
      841 ataaattact ggagatcctt tatatcgtga tgggactcgt attgatttac actggagtaa
      901 gaaatttaac agataaaacc aatcctcatc gttatgggtc agcctttttc tggacggtct
      961 taggggtagt tattgcagga ggaagatggc ttccggcaat cgtgaacggt ggcttgatct
     1021 ttgcgatgac cataccagca attgctaaga aagtcagcaa aggagaaagc cggcttccat
     1081 caaaagaata tatggaaaaa atgtcagaca aactaggaat gaaaattttt attccagctt
     1141 tgagtatcgg tgtctttgcg atcttgtttg ctctatttac caatttaggt gctttagtcg
     1201 gcgtgggcgt cggtgttttt gcagccatgc taatcttgat gttcttttct cgagataatc
     1261 gaccaagtac atttttagat gatgcagcag atatgctggg cacagtaggg ccgttaagca
     1321 tgttgccaat gcttttagct tctctaggtg cggtatttac aagcgctgga gttgggacag
     1381 tgatttccaa tgcagtaggt acgatcgttc cagaaggaaa tgtgaacatc ggcatcatca
     1441 tttacgctct tggcatggtg atatttacgg taatcatggg gaatgcattt gcggccatca
     1501 ccgtgatgac ggttgggatc ggtgcaccat tcgtatttag tcatggagcg aatccagcgt
     1561 taataggaat ggttgcattg acttgcggtt tttgtgggac cctgctgaca ccgatggctg
     1621 ctaacttcaa tatcgtgcct gtagcgatgc tggagatgaa agacaaatac ggagtgatca
     1681 aaaaccagtt gtttattgcg ttatttatgc tagttttcca aattgcttat atgattctat
     1741 ttaaataata gaagagaggg gttgtgacag aataattgtc gcaacctctt ctaacattgt
     1801 agtggaacac tattttttta taagaatata ttaagtttga tctgttatgt tatttaaact
     1861 attgtttcct ttatttacgc tgttcaagga aaatggtatg ataaaagata accatatgtt
     1921 aaatcaatcg gacggaggaa caacagatga aagtactagt aacaggtttt gatccatttg
     1981 gtggggacaa agtgaatcca gcctatgaag cagtaaaaaa aatgccagat gaaatcagcg
     2041 gtgctgaaat catcaaagtg gaagtaccga ccgtattcga aaaaagcagt caagtagtca
     2101 aagaagccat ccaacagcac caaccagatg tcgtgatctg tgtcggacaa gcaggtgggc
     2161 ggtcagcagt ctcgttcgaa cgtgtcgcaa tcaatctagc agaagcaaga atccctgata
     2221 atgaaggaaa tcagccattc gatacggctt tagaagaaaa cggtccagcc gcttacttta
     2281 cctcattgcc aatcaaagca atgacaaaaa atgtacaaga tcatggtcta ccagcgtaca
     2341 tctcttatac agcaggaaca tttgtctgca atgatatcat gtatcgtttg cttcatttga
     2401 tcgaaacaca attcccggca attcgaggtg ggttcatcca cgtgccattc tcacccgatc
     2461 aagtgatcga tcgtccagtg ggtacgcctt ctatgtcttt agaagacatt gcttcttctt
     2521 tgacttatgc agttgaagca gcagtagaaa ataaagaaga tatttccgga aatgccggaa
     2581 caactcacta atttagcaag aaacactggc tggttgctaa cgttatgtta gtagccagct
     2641 ttttgtaatt agaaaggaaa gaaacagccg tggaagaatg gcaatccgtc tttgaagaat
     2701 ggtttccaaa agaaatcagc aaaagctatc ccatcaaaat ttctaaacaa tacacaagca
     2761 gtcaacgttg ggagatctat gcaaaattaa cgaaaaaaca gcgggaacta gtcgataagc
     2821 accgccgata tctaatcagc tctcgtttta tggaagaaca ttatttagct gcaacagatt
     2881 gggtgttttc tgacttcaag atcaatccgt tttttcgaac aaaacgaagc cagcaaaaat
     2941 tgtattgtga gtgtggtaga gaattaaaag tgcaatacat cgttaaatcg ccaaaaaccg
     3001 gaaagatcct aaaactaggc atcaaccact ttgccgatca cctgcatgtc tctccgacag
     3061 tggcagcttc gatccatcag ggaatgacaa aagttgatct ggccttagac gaattattat
     3121 ggcttaagca gaagaatatc gattttcctg aaggactatg gcaaaaatac tgttttgtct
     3181 tgtaccaaaa tcgtcggatg aaacagccat atttaccgga tataaaatta gctcagcggc
     3241 tcgcagaatt tcgccaagta gaaatgccga tttacatagc ggattatcaa gcattggaaa
     3301 atgaaatcaa gaaaataagt gaacacatca atgggcagtc aaaaaagcga cagataaaaa
     3361 aagaactatt tgacgacttt gcagaagaat tagtaaagga tgtagaagaa ttcttgatca
     3421 actatcgcgc atttttacgt aaagattggc aatccatcgt atatgaagaa gtgccagtac
     3481 atcctaatgc ttattttgaa acatttattt ccgtgttgag aaagacaaaa cgtcaacgaa
     3541 caccagaagt cactgctcag atggaatatt ttgcgaaaaa ccaacgattc atccaaccaa
     3601 aaatctatct atttatttgg aagcaatatt gtcgctatgg ttttacagaa gggttcttcg
     3661 acagcattcc tcggatagtc agaaatggtt ttttaaaagt attgcgtaaa gaacgagaag
     3721 ctatacaatc tgctgataag aaagaccgta cagtctcaaa agaaaaatgg caactagtag
     3781 tgaaagatat ccaatctgga aatgtgcaag agacaatcga caaatggaaa ggaaaacatt
     3841 atcgttttac tgaagctcag aaacaagcgt tggaatatta tcagaagtta gaagaatcac
     3901 tgcgtttcaa tgatgaggca agaaagtatc tgaaggaatt actataaaaa atagaggctg
     3961 ggacagaagc gtttaactcc aagaaataag aagaaattca cgaaaattgc ttttcaaatt
     4021 tttgtgaatt tcagcttatt tccgaaggag ttgcttctga ttccgccgtt tatacgtgtt
     4081 tagagcgtga gacaaaagtg ttctttactt ttgtcccacg ctcagttttt ttatgcgtgt
     4141 tgttgtgcgt tgtagtattc ttcttccatt ttttgcatac gttcacgttc tttttggtta
     4201 taccaaggtg aaacggtaaa tgcaaggaca tcattcagca agtaaacaga gctgttcacg
     4261 aacatcgcca aacttgcaga tccatgcgta aatgaaatat accaaaggac catttgagca
     4321 agccctgaag ctaaccacca gtaatattgg ttgttgtaac gcaagaaact gatgatacca
     4381 gcagtcaagc taatagaaaa agcaatggca tctacccacg gccgaggatc atctgtataa
     4441 cgaccaatca ggaatcctga aacaagatag acaaggaaag tactgatgat tgcgataaac
     4501 cagctttttc cagtgaattt tcggattttt gaagccatat tatgattcca ttctttactt
     4561 aacaatacgg ggatatccaa tgtgatcata taagcaatct gttcaccgat acttaaatag
     4621 ttctttgctg aaaacccaac gataataaaa caaatcgcag acaaaatacc taaaataccg
     4681 ttgactgatt ttgccgcatt gatggataaa atacacaata caccaagcgt ggtaccgata
     4741 aatgtaataa cggacagcca agtgatggga ctattgacaa gagtcatgac ctggcagcca
     4801 agactgaaaa agaaaagata ataattttgt tgcggccagc cttttagctg atgagctaaa
     4861 aactgaaaat atgacttcat gtatgaggac tccttaagac actaaatgta gtgtgttctg
     4921 tatttactaa cacaatctat tgtatgcttt
//
LOCUS       DARDHA010000173         4882 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000173 DARDHA010000000
VERSION     DARDHA010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.177"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(406..780)
                     /locus_tag="SDA10_002499"
     CDS             complement(406..780)
                     /locus_tag="SDA10_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG4241373.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
     gene            complement(803..2203)
                     /locus_tag="SDA10_002500"
     CDS             complement(803..2203)
                     /locus_tag="SDA10_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HEG4241374.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            complement(2207..2617)
                     /locus_tag="SDA10_002501"
     CDS             complement(2207..2617)
                     /locus_tag="SDA10_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4241375.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            complement(2635..3126)
                     /locus_tag="SDA10_002502"
     CDS             complement(2635..3126)
                     /locus_tag="SDA10_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG4241376.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            complement(3136..3927)
                     /locus_tag="SDA10_002503"
     CDS             complement(3136..3927)
                     /locus_tag="SDA10_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG4241377.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            complement(3920..4693)
                     /locus_tag="SDA10_002504"
     CDS             complement(3920..4693)
                     /locus_tag="SDA10_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4241378.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
BASE COUNT         1552 a          995 c          647 g         1688 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaactattg aaatgctgat accaaaaaat
      361 gaagtacttt tcgttcatac caacttagag cacttcgtta tctatttatt tcttcatttg
      421 gcttatctct ataatctcat gatttggtcc gtagaaatta aagtaacgga ttccattttc
      481 ccagaacggt aattccataa tatttttatc taataattca tatcctaaat cttgaattga
      541 attgaatacc tcatctatgt catccgtatc taatgctaga tgatcaatgg ctcctctaca
      601 ctcagtagtt ttatcttccc atatttctat tatcgttcca ttcaactcga aaaagccgac
      661 tttacttccc acattcattt cttcattaat cagactaaac cctaaggatt cataaaagat
      721 ttttgaagct ccaaaatctt ttgttggaat tccaatatgt tgtaaaccat ttaatttcac
      781 ttacatcatc ccttcaattg ttttaaaatc cacattcata atttattgaa tagtctagtt
      841 tatctacctt gattgctccc tccgtaacag tcatacttaa tgctgttccg actttatcat
      901 acattctagt acttaaagca acattatcaa tatataaaac agctatcgta tcatcaacaa
      961 ttaactttag ttcatattct ttgcctattt ccaaaaatag tggtcgttgc agccccttat
     1021 caaacatttg aggccaagga tagttcggag tttttccgaa agtaagctga ttattaatca
     1081 atgaaatacg aaattcgtat ccttcttgag tatcactatc tacaaataat tttaaagcaa
     1141 actcctttgt taacgatgta atagtcaaag ttgtttcaaa tagaaaataa tcttcgattt
     1201 taggaacaat tactctttct ttttttccaa attctttctc taacttaaaa cttgaaaatg
     1261 gagtgatttc tttaaaattc tcaattattt tttcaggcag tttaacacca agagttttgt
     1321 ctgtcttttg aataatttga tgtggaacaa gtgcaccgcc ccaatcccag ttattttgat
     1381 cgttctcccc ttcttttgtt gccacccagc cagttagaaa acggccagtt tcattagttc
     1441 cgactgttct tgctgcataa taggcttttc cgtcgaaagc ttcgtctact ggtgatttcc
     1501 aatctgaaaa taatcctgct ccgactcgat atttagtctt tttatcttca caatactctg
     1561 aaaaaagtaa ataccacgtt ccatcaattt caaatatatc aggcatttca atcatgttaa
     1621 attcattatt agcccagtag tctcctttga aattccaatc ttttaaatct tcggattcaa
     1681 aatagaccaa tctccccgtc ggctttttct tacttgctgg ttttctagct cctagtatca
     1741 aaacgtattt tccatgatct tgatcaaaaa ttgcaaaagg atcgcgccaa tcattcttat
     1801 catatccttc ttgaggaaca agttttgctg cttcgccatc ctttttccaa tgaattaaat
     1861 ctttagaact tgcaatcatt aacacttctg aagtttttcc atccaactta gctttgcgat
     1921 tatgtccagt atacaaagct aaatactcat tgattccttg gacaatactt ccagcgtaaa
     1981 tgtattgatc aatgtcatcg ttgccaccac gatgaattac ttccccgtat tcttcaaatt
     2041 tcacaaaatc agtggttgta actagtgacc acccaaaagg atcagttaaa ggtttaggat
     2101 ttctcgtatc tcgttgatga aatagataaa aagttccatc tttataaaaa ggcatgcaat
     2161 ccccaaacca aaaatcttta ggcttgtaaa aaattttttg catttatcac tcatcctctt
     2221 cgttagtatg tctttgcata tctgcaagac acgcaattgt attcatattt cttagatttt
     2281 cgatgatatc atcgcttttt tttccttgta atctcaaatt gacagcctca atcaaaattc
     2341 ctaaattcat gccaccaaga agttgaaacc cattttgaaa tacttcactt cttttagcaa
     2401 attcgttgaa aggtgttcca ccaatcaagt cagcaaatac cataacttct tgatttttta
     2461 cttgagaaac tattttttca atgttttcac ggaattcttc aatgcctgca tctccaagtg
     2521 ttaccacatg acaatttaaa ttatcgccaa aaaacatttt taatgtatct acaaatccaa
     2581 tggcaatatt tccatgagat gtaattataa actccatcca ctcatcctct gttcttaata
     2641 aataaaatcc tttaaaaatt cttttgaatc tctaggagtt tgtatcactg tcacttcaac
     2701 tccatcacta attagttctt taaaagcctc tctctcttct tcagtaatat ttatcgtcgg
     2761 acggatgcgt tctgttccat tccttcttga taaatttcct acgtttacac ttgttattgg
     2821 taaaccgtta tcaatcaaaa atttaacatc gattggagaa actgcaacaa taagaacttt
     2881 ttgtccttca tatttaccag aaagaatatt tttaactgct acttcccttg taattattga
     2941 agttctaaca ttaggtggtg cagcgaggcg aactacagct ttttttacgt catcattggc
     3001 tacttcatta ttaataacca taattctgtt agcactcaat tgatttgtcc attgtgttgc
     3061 tacttgtcca tgaatcattc gatcatcaat acgaacatgt ataatacctt tcattttttc
     3121 atccatattc aacacctact tcaaaatatt taacgctctt aacgcaatag aaactactaa
     3181 gattaataga attacataaa ctggtttaac ctttcgtcct aacaaataat acacaattgc
     3241 tactacacat gcaggtaata aaccaggcat aacttgatca aagatttcat ttccggtcat
     3301 tgttaagtcg ccttgtttaa attcaaagcc aacattcact tttacaactg tggcaatgag
     3361 tgcaccgacc accattaacc ctaaaattga tactgcatca gtaatagact ttaattttgt
     3421 ttcaatttga gttagtaggc tcgcacccga ttctttccca gcaaggtata aaggataagc
     3481 agtaatcctt aataaaattg atgccagtat ccacaaaata attccaacaa aactaccttc
     3541 caatgccata ttagctgcaa ttgctccaaa aatagtccat ggaataacta caaatacact
     3601 atctccaatt cccgctaaag gacccattag acctgttttc agagcactta ctgcttccaa
     3661 agacttattt ttttcttctt tttcaattcc aacattcata ccaataatgt aaggcgctgt
     3721 atacggcgta gtgttaaaaa attgtaagtg agctaaaacg gattgtttca ttccttcagg
     3781 atcatcttta tatgtgtcct tgataaaagg aagcatagaa tacgcataac ccaacccttc
     3841 cattttttca taatttaatg aagttgcacc attgagaatc caccggaaaa ttacttgttt
     3901 gtcgattttt gtcagtttct tattcatctt cgtcaaaacc tcctgttgca gctggtgcta
     3961 cttgagtatt atttgatcta tttttatata cgattagtgc gattgcagcg ccaagtaacg
     4021 aaatccctaa aattggtatt tggaaataaa ctgccaaaac gaaaccaact aacaaatagg
     4081 caaagtaatc ttttgtaggt aaatatttaa gtagcattcc tatcccaaca gcaggtaata
     4141 gtccccctgc tacctttaaa ccaccggtaa cccattcagg aatataattg attgcacttc
     4201 ccactaacgt tgggcctaat atcacagtta acaaaacagg aattccagat acaaagcttg
     4261 tattaattga tgccaaatat tgcatccact ctaatttttg ataatcttct ttttgtgcga
     4321 atttttctgc cttttgttga aaaaaaatgt ttgttgacca ctttagaaca tctagttgta
     4381 caattaacat tgctaccggt attgctaaag ttattcctat agaagcatcc tggttagttg
     4441 agattgctaa ataagtaccg atcaatgctg ctgtttgata atccggtact gatgcaccac
     4501 caaaactagt aatacctagt gtcattaatt gcaaagtccc accgatataa agtccagttt
     4561 gaatatctcc cataataagt ccggtcaaga aacctgcaac gacaggttgg gaggttccga
     4621 ttttgggccc ttccttatcc cagttaatga aaaatccata caacacaact aaaaatgttt
     4681 gccaaaacgc cattttgttc tctcctttct tcttatactt ttatataagc aattactgtg
     4741 ccaactaaca ttatccaata aagcgggaaa atacagtaaa tattaattaa atgagcgtct
     4801 aaatatactt tggacaccct ttcatttttt tagacgctag tatgctattt agaaaatagt
     4861 ctgctcaaaa attgtataga tt
//
LOCUS       DARDHA010000174         4849 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000174 DARDHA010000000
VERSION     DARDHA010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.178"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            55..1695
                     /locus_tag="SDA10_002505"
     CDS             55..1695
                     /locus_tag="SDA10_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEG4241379.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            1849..3171
                     /locus_tag="SDA10_002506"
     CDS             1849..3171
                     /locus_tag="SDA10_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEG4241380.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3239..3673)
                     /locus_tag="SDA10_002507"
     CDS             complement(3239..3673)
                     /locus_tag="SDA10_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4241381.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(3738..4575)
                     /locus_tag="SDA10_002508"
                     /pseudo
     CDS             complement(3738..4575)
                     /locus_tag="SDA10_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
BASE COUNT         1483 a          950 c          931 g         1485 t
ORIGIN      
        1 atcatcatct gacttctata cttcgtttgt attaaaactt aggaggaatc attcatgctt
       61 acagggttaa acttattaaa taatcctttt ctaaataaag gaacagcttt tacaaaagaa
      121 gaacgggcaa aatatggaat cactggtatg cttccaagta ctgtccaaac actggaacaa
      181 caatctgttc aagcttatgg tcaatactta agcaaacaaa cagatttgga aaaacgtatc
      241 ttcttaatga atctattcaa tacgaatcgg acattgtttt ataaattgat gggcgaacat
      301 ttagttgaat tcatgcctgt cgtttatgat cctgtcgttg cggattcaat cgaacaatac
      361 aacgaaattt tcttgaaacc gcaagatgct gcatttcttt caatcgacga accagaaaac
      421 atcaaagcaa gtttgaagaa cgcggcagat ggacgagaca ttcgtttgat tgttgtgact
      481 gatgcagaag gaatcttagg tatgggcgac tggggtgtga acggtgttga catcgctatc
      541 ggcaaattga tggtctatac tgctgctgct ggtattaatc cagcacaagt tttgcctgta
      601 tcgatcgacg ctggtactaa taatgaagaa ttattgaata atcccttata cttaggcaat
      661 cgtcatgctc gtgtagaagg cgaaacttac tatgaattca tcgatcaatt cgttcaatca
      721 gctaccgaat tattcccaga attgttattg cattgggaag attttggacg aggaaatgct
      781 gcagcgatct tagaaaaata cgaagatcaa atcactactt tcaacgacga tatccaaggt
      841 acaggaattg ttgtcctagc aggtgtatta ggcggattga atatatccgg tgaacaattg
      901 aaagatcaaa taattttgac atttggtgct ggtactgctg gtgtgggtat cgcaaacatc
      961 cttttagatg agatgatccg tcaaggtgta cctgaagaag aagcacgcca acatttctat
     1021 caagtagata aacaaggttt attgtttgaa gatacagaag gattgactcc tggtcaaatc
     1081 ccattcgctc gcaaacgctc agatttcact aatgctgatg aattgacaaa cttagaagca
     1141 gttgtaaaag cgatccatcc gacaatcatg attggtactt ctacccaacc tggagcattc
     1201 actgaagaaa tcgtgaaaga aatggctgct catacaccac gtccgatcat catgcctttg
     1261 tctaatccaa caaaattggc agaagcaaaa gcaaaagatt taatagaatg gacggacgga
     1321 aaagcattag ttggaacagg tatccctgct gctgatgttg aatataatgg tgtaacttac
     1381 caaatcggtc aagcaaataa tgctttgatg tacccaggtt taggacttgg tttgattgct
     1441 tcaacagcta cacgagtaaa tgctgaaatc atttctcaag ctagtcgagc attaggcgga
     1501 atcgttgatg taacaaaacc aggagcagct atcttgcctc cagtagcaaa aatcactgaa
     1561 ttttctcaaa cgattgctga aactgtcgca aaatcagttg tcgcacaaaa cttgaatcgt
     1621 gaagaaatca cggatatcaa agaagcagtt gaatctgcta aatgggttcc tgaatataaa
     1681 tctttagaag attaagatcg ttatagtaaa agaacataca gaaactttgt atgacgtcta
     1741 aaaagaaaaa agcgactgcg gcaaaacagc ggccagccaa ctcctgctct tttgtcgctt
     1801 ttctttggct tttttcatat gatacagaat aagaaaggat ctgtaaaaat ggcacaaaaa
     1861 acaacaactg caactgcagt tgaggaaaag aaaaaagaag gtttttggtc cacaaaaatc
     1921 accggcgtaa gtttgccact ttatttgatc atggtccttg tcctgatcat tgcaatggct
     1981 ttagggaaat taccgacgaa tatgattgga ccaatcgcag ttttggttat tttcggtaat
     2041 ttatttcatt ttatcgggac aaaaatccct atcgtcaaaa gttatttggg tggtggttcc
     2101 gtttttgcga tcttcgcttc tgctgcgatt gctacatttg ggatcttacc tgaatatgta
     2161 gtgaaatcca cagaaaactt tgtcagcaat atgggctttt gggactttta tattgctgcc
     2221 ttgattactg gtagcatcct tggtatgaac cgtaatctat tgatgaaagc ttctgttcgt
     2281 tttattcccg ttgcacttat ttcaatggtc gtctcctttt ttgccgtagg actcgtgggc
     2341 atgcttatcg gaaatggttt tgcaaattcc gttttataca tctcattacc tgcgatggct
     2401 ggtggtgttg gagccggagc cgttccttta tcaacgattt atgcaaatgt tttaggtacg
     2461 gatgcttctt ctatcatttc ccgcctgatt cctgcgacag caatgacaaa tgtcttagct
     2521 atcatcggtg cagcattagt tgctcgtgct ggacaatcaa tgccaaaatt caacggcaat
     2581 ggtaaattaa tggaaaaagg cgatcttggc gatggaaaac ctcgaactgt gaaaccttcc
     2641 attccacagt taggtgttgg tttgatggta tcattgactt tcttcatctt aggacaaatt
     2701 ggtaactatt ttgttcctaa agtccatgct tacgcattta tgattattat cgttgtcatc
     2761 tgtaaaatcg ccaacctatt accagagtat tatgaagatg cagctatcat gttcaataac
     2821 ttgattgtca aaaacttgac agcagctgtt cttgccggta tcggtattgc gttgctgaat
     2881 ctgaatgtat tagcttctgc tttgacatgg caatttgttg tcttatgtct gacaagtgtt
     2941 atcgcgattt cagttgtttc aggatttgtc ggtcgtttgt ttggtttata tccaattgaa
     3001 tcaactatta ctgctggctt atgcaacaac tcgatgggcg gaacaggaaa tgtagccgtc
     3061 ttatctgcag ctaatcggat ggaacttatt gcttttgctc aaatgggcaa tcgacttggt
     3121 ggtgccatcg ttttgatcat ctctggtttc ttgatgcaat tactttctta gtcaaaaaaa
     3181 caaaaatacc tgatcatcaa gtatcggctt ggtgatcggg tatttttttg ataaagattt
     3241 acaggatttt caaaaatctt ttttgcattt tatcggaatg ataaccgatg tgttcataaa
     3301 attggtgtgc ttctactctg tattctgcag aattaagcct tattgtcgtt attcctcgtt
     3361 ttttcgcttc ttcttctacg ccagtcatca aagcctttcc gatgcctctt ttttcagcat
     3421 atttagacac agcaagtgcc ataatattga acattggatc gctaaaaatc gattcatagg
     3481 actccgcttg tacatagcca gctgcttttt tagaagtagg atcttcataa accaagaaaa
     3541 aatttttctc tggctccttc agcagtttct tcatttgtct caccgtaacg gttaatggta
     3601 tatcataccc taattgatcc gcattgattt cacgtagtgc ttctgcatct tctaaacgca
     3661 tttcccgtaa catccaaatc accagccaat ttctgatttt ttaaaatgaa tttttttatc
     3721 atagtatact ctttttttca tttagaacaa tatgtttcag tgattttgac ttgcgttagt
     3781 ctagattctc ctaatgtttg taagatgaca aaggcattgt aacaattgac caaaagaacc
     3841 acgaggacaa ttacttgaaa aataaaggtc actttttttg gagacaaaac cctgctaaat
     3901 cttgctcccc acaaacctcc aataacagca gcggggataa cataaaacaa catagataaa
     3961 tcaaagcgca taaagctact tgttaaagca atcgttacca attttgccag ttgagaaaag
     4021 aaaatcgtag atagagaata tagtgttgct tcttttattg gcaatgcaaa catcagcatc
     4081 aacaaagaaa cattgactgg tccaccacca atacctaaaa aactagctaa aaagcccaga
     4141 acccccccca catataaggt accaagtcca tgacgtcaaa tgaaattttg gctgatgatg
     4201 cttggtgtag aagaacgcaa aaaccaaggt caataccgtc aaaaaaattt ggatcagctg
     4261 cacatgcttc tcgttttcaa ataactgtaa aaaaacttcg aatacaatgt tgccgactac
     4321 tcctccaaca acagctccac tagataccca accgacaatc tgccaattca gtgatttccc
     4381 gcttgatact tgtcgcacag tcgaaacaat ggacatcgtg aataccgcaa ctgttgagta
     4441 aaaagaaatc cctgcaacag aatgggcgcc gataagatca agaatcggtt tgatcaacac
     4501 gcctccaccc atcccggaaa tagcgccaac tgtgttcgcc agaacgatca ccacaaaata
     4561 aatcagacca atcatcttgt atttcctttc tcttgtttat atcttttagc gattgttact
     4621 cttttctttt attatacagt aaaagaaaca aacgaggctt cgaaagcttt tctacttttt
     4681 aaggaaattt agtgctaaac gaaaagaggt tgtgaaaaag ctgtcctgca tcaagtaata
     4741 agaacagcca aacaaaaata gctcctcata attttcgttt ggttgaactt attaccgcag
     4801 atgcaagctt ttgaacaccg tttaagaaag aggtcgtgag cctgccgtt
//
LOCUS       DARDHA010000175         4788 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000175 DARDHA010000000
VERSION     DARDHA010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.179"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            564..989
                     /locus_tag="SDA10_002509"
     CDS             564..989
                     /locus_tag="SDA10_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241382.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="SDA10_002510"
     CDS             1019..1183
                     /locus_tag="SDA10_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241383.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="SDA10_002511"
     CDS             2141..3118
                     /locus_tag="SDA10_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG4241384.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="SDA10_002512"
     CDS             3128..3625
                     /locus_tag="SDA10_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4241385.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="SDA10_002513"
     CDS             4001..4282
                     /locus_tag="SDA10_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241386.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="SDA10_002514"
     CDS             4284..4700
                     /locus_tag="SDA10_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241387.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1758 a          816 c          785 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtatacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DARDHA010000176         4775 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000176 DARDHA010000000
VERSION     DARDHA010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.180"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..258)
                     /locus_tag="SDA10_002515"
     CDS             complement(<1..258)
                     /locus_tag="SDA10_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241388.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="SDA10_002516"
     CDS             complement(274..924)
                     /locus_tag="SDA10_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241389.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="SDA10_002517"
     CDS             complement(935..2107)
                     /locus_tag="SDA10_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEG4241390.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="SDA10_002518"
     CDS             complement(2126..2998)
                     /locus_tag="SDA10_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG4241391.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="SDA10_002519"
     CDS             complement(3020..3940)
                     /locus_tag="SDA10_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241392.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="SDA10_002520"
     CDS             complement(3940..4209)
                     /locus_tag="SDA10_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241393.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="SDA10_002521"
     CDS             complement(4206..4586)
                     /locus_tag="SDA10_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEG4241394.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
BASE COUNT         1250 a         1048 c          789 g         1688 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgt
//
LOCUS       DARDHA010000177         4744 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000177 DARDHA010000000
VERSION     DARDHA010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.181"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            72..1118
                     /locus_tag="SDA10_002522"
     CDS             72..1118
                     /locus_tag="SDA10_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG4241395.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            1135..2358
                     /locus_tag="SDA10_002523"
     CDS             1135..2358
                     /locus_tag="SDA10_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG4241396.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            2447..3334
                     /locus_tag="SDA10_002524"
     CDS             2447..3334
                     /locus_tag="SDA10_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEG4241397.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            3420..3650
                     /locus_tag="SDA10_002525"
     CDS             3420..3650
                     /locus_tag="SDA10_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEG4241398.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(3707..4672)
                     /locus_tag="SDA10_002526"
     CDS             complement(3707..4672)
                     /locus_tag="SDA10_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEG4241399.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         1604 a          788 c          966 g         1386 t
ORIGIN      
        1 aaaaataggg cttaagtttg agtgacagtt tgtaggctat gttatactat ataagtgaag
       61 ggggacgtaa ggtgaaggta ttattatatt ttgaaagtga aaagatgctt gcaaaatctg
      121 gaatcgggcg tgcgcttgat catcaaaaac gtgctctgac ggaagtcggt atctcttata
      181 cactagatga aaaagaagat tatgacatcc tgcacatcaa tacatacgga atcaatagtc
      241 ataatatggt caataaagcc agaagagaag gaaaaaaagt tgtctatcat gcgcattcta
      301 cagaggaaga tttccgaaat tcctttatcg gatcgaacca gctttctcct atcgtaaaaa
      361 aatatctggt aggattgtac caaaaagcgg actatctgat cacgccaaca ccttattcaa
      421 aacagttgtt ggaaagctat ggtatccgtg tgccgattca agctatttcc aatgggatcg
      481 acttagagaa atatcaccct gatccaatga aagaacaaaa gttcagagaa tatttcaaac
      541 tgtctccgga tcaaaaagta atcatttgtg tcggtttgtt tttcgaacgt aaaggaatca
      601 ttgatttcgt agaaattgct aagaagatgc ccgaatacac atttatttgg tttggtcatg
      661 ttcccatgta ctcgattcct cggaacatcc ggaaaattgt aaaagaagat catcctgaaa
      721 atgtcctgtt tcctggttac attcgtggag agatcataga aggtgcgtat tcaggtgcag
      781 atctattctt tttcccttct tatgaagaga cagaagggat cgttgtttta gaagctttag
      841 caagcaaaca aaacgtactt gttcgagata ttccagtgta taatggctgg ttagaagata
      901 gcaagaactg ctatatgggg aagacaaacg aagaatttat ccgactgatc caacagatta
      961 ccaatcaaga actaccgagt acgacacaag ccggttatca aacagcaaaa gagcgaagta
     1021 tcaataaaat cggtgaagaa cttaaaaatg tatacgaaag tgtactgaat gaaaaagtct
     1081 ctagcaaatt aaaaaaagta aatcaggtca aggactagtt tggaggcgaa aaacatgaaa
     1141 atcggctttt ttaccgatac ctactttccg caagtcagcg gagttgctac atcgatcaaa
     1201 acattaaaac aagaattaga aagacatggg catgaagtct atatttttac aacgaccgat
     1261 cctaatgcgg ataagttgga aaaagatgtg attcgaatgc caagtgtacc atttatttct
     1321 ttcaaggatc gaagagttgt tgttagaggc atgtggtatg cttatttggt tgccaaagaa
     1381 ctagaactag atcttattca tacccataca gaattcggag cagggatact tgggaagatg
     1441 gtagctaaga agttgaaaat acctctgatc catacttacc atacgatgta tgaagactac
     1501 cttcattata tcggtaaagg aaaagtgatc aggcaatccc atgtaaaata cttgtctaga
     1561 ttatttgcaa atcatacgac aggtgtagtt tgtccaagtg aacgtgttat tgatacatta
     1621 cgcagctatg gtgtaatagc accgcttcgc gtgattccta ctggtatcga cattgaaaaa
     1681 ttcaaacgtt ctgatatcac tcagcaggat atcagcaaat taagggagtc actaggtctt
     1741 caggaaaacc atttgatgat tctttcttta agcaggattt cttatgagaa aaatatccaa
     1801 gccttgatta atgggtttcc tcaggtcatc tccgcttatc ctaacgccag attagttatc
     1861 gtaggaaaag gaccttatgt agaagagtta gaggaattga tctcagaact gcagttagaa
     1921 gaatttgtgc agttcacagg agaagtggac aacaatgatg tagcacttta ttataaagcg
     1981 gcagattatt ttgtcagtgc ttctacttca gaaacacaag ggcttactta tacagaagcg
     2041 atggcagccg gaacaccttg tgtcgtggaa ggaaatgctt atttgaaccg attgttcgat
     2101 catccttcat tagggaaaac ttttgaaacc gacgaagact ttgcaccagc acttattgat
     2161 tatatttcca gtgaagttcc taaagatcca aaagttttag aagctaaact ttatgatatt
     2221 tcatctacac atttcggcga agctatgatc gaattttatc aagacatggt tgtttatcat
     2281 gaagaagaga tgcgaagaaa agaagaagaa gactcgattg aacgaatcaa aataaaatta
     2341 aattcattta aaagataaaa ttcaaacatt ttttatgaaa aaatccttgt ttctagcgta
     2401 tactagcata gaagcaagga tttttgattt ttaggaggtc gaaaaaatga aacttggttt
     2461 tatcggaaca ggcgtcatgg gaagtgctgt cgcacgccat ctactagaag caggacacga
     2521 agtagctgtc tataatcgga caaaagcaaa agctgatcct ttagtaacag aaggagctat
     2581 ctgggcagat acaccaaaag cggtagcaga acaaagtaat attctcttta caatggtagg
     2641 ttaccccaaa gatgtagaag aaatctacta tggacagtcg gggatttttt ctgcagatat
     2701 ctcaggacat attttagttg atttgactac aagtacacca agcctggcag aaaaaattgc
     2761 aaaaacagca aaagaaaaag gcgcagatgc gttagatgca ccggtatccg gtggtgactt
     2821 aggagcaaag aatggtacac tgacgatcat ggtcggtgga gaagaagccg tttatgatca
     2881 agtactgcct ttattcaaag aatttggtac gacatttaca ttgcatggtt ctgctgggaa
     2941 aggtcagcat acaaaaatgg ctaatcagat catgattgca ggtacgatga caggaatgac
     3001 ggaaatgctt gtctatgcac aaaaaaacgg acttgattta aagaaagtga ttgagacgct
     3061 ttcaggtgga agtgcagcta actggtcttt aagcaattat tctcctcgta ttttaaaaga
     3121 agattacaca cctggttttt ttgtcaaaca ttttatcaaa gatttaaaga ttgcattaga
     3181 agaagcagaa aaaatggatc ttgtattgcc agcaactacc caagcattga aactatatga
     3241 agagttggca gataaaggtt ttgagaatga cggaacccaa gctttgatca aactttggtg
     3301 gcctgaagga aaaataccag aaaaaaaatc ataaaaaacg agttttttta gagaaaatca
     3361 atgcaagtag gctatgcttt tgatacaata taacagaaat aagaaaagga ggacctccga
     3421 tgacacattc acaattagtc gcaatcataa aacgactgga ggctatggtc gagtcagcag
     3481 ataatgaact gcaagttcgc cgttttgaaa aagaaggcgt agagaagtgt acagtaactt
     3541 atgataaagc gaccgataca tttgaattaa ctgaaacaga tactcatcag caatacgaat
     3601 ttgataatat cgatatcgta gcgatggaaa tctatgactt aatccaataa attttaggat
     3661 aattgacatt tgaagttgaa gatgcctaaa agaagccgag aagcaactaa tttagttgct
     3721 tttcagcttc ttttttgttt aacagttcaa tttcaaactg ttctttttct aattttttgc
     3781 ggtaactgat caagatttgt acaggatcga tttcttcttt tgacaattga cgtttcaaag
     3841 ataaatgagc ttctttcttt tcataataga agtcaggaga aacgtcacca gaggtaatca
     3901 ttgtttctgc aagttgacca taacaaacga ggtagtgctt tttctgactc tcatctgctg
     3961 ctaatggcat gctgaaggtg agcgatggtg ttcttaaaaa ctgcttttgt tcctggatcc
     4021 gtctaataaa ataagtaact aattcgatat ctttttgaag cataccggct ttttcgaaat
     4081 tcaactgata gatgaaatgt tgttgtcttt ttttcaagta agtcagtagc tttttatttc
     4141 ggccttgaaa cagcccttgg agttcctttt ttttctgttc aaaagtcatt tcctgtaggt
     4201 caggaagctg gactcgcaaa gctaacaaac taatttcaga caaccaaggc atttgataag
     4261 tttcttccat tatacgcaaa atagacggca tttttttata gagacgaaaa ggcccgtatg
     4321 ttcgagccgt aggagtatct gttaatacaa atcccttatt ggtaatatgt acataattat
     4381 aattactgaa ataattcatt tgtcggttat aaaaaggacg gtaatgttgg atcaattgac
     4441 actcgagcaa taaagcatct aattccgtgt ctactacgac gaaatcaaaa tctgttatat
     4501 gatgaatcat acgtaatact ttttttgaat gctgtttatt ctgatgaaaa tagctgctta
     4561 ctctattttt gagccgtttt gctttaccaa catagatgac cttaccatct cggtctttca
     4621 taagatagac acccggcagc atggggagag ttttcatttt ttcttttaac ataggatctc
     4681 cttttacgct ttgaataaaa agactataac aaattttgaa ataatggaca agttttaggg
     4741 aatt
//
LOCUS       DARDHA010000178         4710 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000178 DARDHA010000000
VERSION     DARDHA010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.182"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            106..357
                     /locus_tag="SDA10_002527"
                     /pseudo
     CDS             106..357
                     /locus_tag="SDA10_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="SDA10_002528"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="SDA10_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEG4241400.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1737
                     /locus_tag="SDA10_002529"
                     /pseudo
     CDS             1654..1737
                     /locus_tag="SDA10_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="SDA10_002530"
     CDS             1786..1869
                     /locus_tag="SDA10_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HEG4241401.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="SDA10_002531"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="SDA10_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEG4241402.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="SDA10_002532"
     CDS             complement(2994..3857)
                     /locus_tag="SDA10_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HEG4241403.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="SDA10_002533"
     CDS             complement(3859..4131)
                     /locus_tag="SDA10_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG4241404.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4357)
                     /locus_tag="SDA10_002534"
     CDS             complement(4148..4357)
                     /locus_tag="SDA10_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEG4241405.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4710)
                     /locus_tag="SDA10_002535"
     CDS             complement(4455..>4710)
                     /locus_tag="SDA10_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HEG4241406.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1445 a          796 c          865 g         1604 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc
//
LOCUS       DARDHA010000179         4665 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000179 DARDHA010000000
VERSION     DARDHA010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.183"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="SDA10_002536"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="SDA10_002536"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEG4241407.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="SDA10_002537"
     CDS             complement(2262..2513)
                     /locus_tag="SDA10_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEG4241408.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="SDA10_002538"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="SDA10_002538"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEG4241409.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="SDA10_002539"
     CDS             3463..3681
                     /locus_tag="SDA10_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241410.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3878..>4665)
                     /locus_tag="SDA10_002540"
     CDS             complement(3878..>4665)
                     /locus_tag="SDA10_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4241411.1"
                     /translation="ALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKYYI
                     SLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLSR
                     RALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHK
                     DGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT         1305 a          972 c          838 g         1550 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaaggtg
     3721 aactactcac cacttagcta aacctgacgg ttcttaacgc ttgaagtggg agcttcttgg
     3781 gaatagagca tacttgatag cgtatttctt tactaacagc ggtgtctctt atttaccaag
     3841 ctatccccgt agttcctacg gttcttgatg attggatcta agccaatccc atagaatgta
     3901 gtcttagacc ttcggtcaga atattgatac tagcattgat atcacgatca tgatgagtat
     3961 gacaaacagg acaagtccat tctcgaattt caagagattt cttgccatct ttatgtccgc
     4021 actctgaaca aatctgacta gacggaaacc atttatctac tttgatgatt tttcgtccat
     4081 accagtcagc cttgtattgt aatttagtca caaactttga ccaagaaaca tcagaaatac
     4141 ttttagctaa tttatgattt cgcaacatac cttttgtgtt taagtcttca atacagataa
     4201 tatcgtgatt tttgatcatt tctgtactca acttattcag aaaatcagta cgttgattca
     4261 ttactttttc atgcaatcta gctactttgc gtttttgttt ttgataattt ttagcttcaa
     4321 atagattggt acctttattt ttagccaata aagcacgcct agacaatttg cgttgttcac
     4381 gctttagttt cttttccatt ttggacgtga atttattatt atcaattttt tgtccatcag
     4441 aaagaatcgc aaagtccata atacctaagt caataccaat tgtggaatta gttttaggta
     4501 attcgataat ttcttcttta cacaacaaag aaatatagta tttaccgcta gaatgacgtg
     4561 atattgtagc ggatttgata atcccttttg gttgtctatg aagcttaatt cttactaatg
     4621 acttcaattt aggaactttg atgaatttac tatcaatcaa agcaa
//
LOCUS       DARDHA010000180         4661 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000180 DARDHA010000000
VERSION     DARDHA010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.184"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..908)
                     /locus_tag="SDA10_002541"
     CDS             complement(<1..908)
                     /locus_tag="SDA10_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG4241412.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIA"
     gene            complement(905..1369)
                     /locus_tag="SDA10_002542"
     CDS             complement(905..1369)
                     /locus_tag="SDA10_002542"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEG4241413.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(1374..2072)
                     /locus_tag="SDA10_002543"
     CDS             complement(1374..2072)
                     /locus_tag="SDA10_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG4241414.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(2059..2445)
                     /locus_tag="SDA10_002544"
     CDS             complement(2059..2445)
                     /locus_tag="SDA10_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241415.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(2592..4262)
                     /locus_tag="SDA10_002545"
     CDS             complement(2592..4262)
                     /locus_tag="SDA10_002545"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEG4241416.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            4445..>4661
                     /locus_tag="SDA10_002546"
     CDS             4445..>4661
                     /locus_tag="SDA10_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG4241417.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTD"
BASE COUNT         1335 a         1021 c          810 g         1495 t
ORIGIN      
        1 aatgcaatgt tttttcttaa tttttctaaa gttttttcaa aaacttctgg aagaggtgct
       61 tcaaagatca tcttttcgtt tgtagtcggg tgcgtaaaac ctaacatttg cgcatggaga
      121 aactgaccat tgcctttcaa agttttcttc ggtccgtata ctggatctcc agctaacgga
      181 taaccgatat acttcatatg cacacggatc tggtgtgttc ttccggtttc tagttttaat
      241 tcaaccagag taaatccttc gaaacgttcc aatacagtaa aatgtgtgac agccgacttc
      301 ccatcttctc ttatcgcctg cattttccgg ttttccttcg aacggccgat tggcgcattg
      361 atggttcctt tttcgtgagg aatctctcca tggactaaag caacatattt gcgcaaagag
      421 gtcttatctt ttaattgctg agctaatgat tcgtgtgcct ggtcattttt ggcaaccatt
      481 agcagtccgg aagtatcttt gtcgattcgg tgaacaatac ctggacgaat aacgccgttg
      541 atcgtagaaa gatccttgat atggtacatc agcgcattga ccattgtgcc atgcgtatga
      601 cctgctgaag gatgaacgac cattccttga ggtttgttta tcaccgctac atcttcatct
      661 tcgtaaacga tcgtcaacgg gatattttct gggaccacat ctaatgtttc tggttcgggg
      721 atcaagacag taatctcgtc tccttgtttt actttgtaat ttgctttgac tggctgtcca
      781 ttgacagaaa ccgctccatt tttcagccac agctgaattt gcgaacgact gtaatcggtg
      841 aataaattag tcaatacttt atcgatccta ccagattcat ggtgaatcgt cgttttaagt
      901 tctgtcattt ttttctttga tccttttcat ctaaaataat ataaattaac atacaaatca
      961 ctccacatac aagtgtcaca tctgctacgt taaaaatcgg aaagctcata aaatctgtct
     1021 ggaacatatc tactacatag ccaagacgaa tacggtcgat aaaattgcca atcgcaccag
     1081 ctagaatcaa actcaatcca atcatgaacc agcgattacc tttttcacga tttttaatca
     1141 tcaatgtgag aatcacaacg actgcaatga gtgtcactat agtaaagaac cacatcttcc
     1201 cttctagcat actccatgcg gctccagtgt ttcgaatatg attcagtgac aaaatacctg
     1261 gtataagatt tttcgtctct ccaagcgcta gattatcagt gatccaccat ttgaccaact
     1321 gatccactcc tactagcaaa gcgctgaata tccaataaat aattaacaaa aaatcaatcc
     1381 ttttctattt gtttataaat atcacgaggt cgtacgatcc cgagcagatt accatcggga
     1441 ttcccatctt tagtgataag tacggcttcc aaagtccctt gctcacgaaa catcgcttca
     1501 acctcgtaaa tatgtgtttt agctggaaca aaatgataat ttgtgtactt tccgttttgg
     1561 atgatcaaat ccaacgctcg tttatttgtt aaatcaaccg gacctttttg gctttctttt
     1621 gctagccaaa acccgatatt acgtaaagta atcaatgcta caaattttcc tttattgtat
     1681 aacggaaatt gtgaatacct tttttctgct actatcttta acagttcaag taacggaaga
     1741 tcttgttcaa aacctgttac acgttttgca aatcgaggca gcacggtttc aggctgaatc
     1801 agttcttgtt cgatcagctg gattcgcttg actgtccaat catttggctc tgcaatgaca
     1861 aaatcgactg cgatccgatc atggacgatc gcattcctta actgagcaag ctgtaaaaga
     1921 tcatcttcgt attttctgat cattaattgt tttctttgag ctaggcgtct gacaagttcg
     1981 gtaaatccca tatttcgtgc atttcccatc tcgtctctca tccatttttc gattcgattg
     2041 aaactgatta aaaaaagatc agagtttgcc ataattttcc tccttgatca aatatgcccg
     2101 gttttttata tagaaaaaga acgcaatcaa cacaataact gcaaaaaaac tgaaaaataa
     2161 attttgtagg aaaacggccc ccaacccagt tgctagaaat aaaaacggat agatttgaag
     2221 aatcaagtaa ttcattgtga tcacataagc aggctttgct tctttcactg tataaacggg
     2281 gctttttagc caataaaagg tgacctgcat cgtaaagggc acttccagat caagcgttat
     2341 cttttgttca tgactgagaa cactgcgttt ttcattgttg attcttagct gaatcgagct
     2401 acccataccg taaaaaccgg tttttcttat aaatgtcgtt ttcatcatac cctcctatta
     2461 tgctttagta tagctcaaaa tacaataaag aaaaagaatg atcctagtgt aaacaaaatt
     2521 aattccctat cgtctaaaaa aagaagctgt agtcaaaata cagctccttc gatggtagat
     2581 tataatcaag cttaaaacaa tcccaacact tctccgtcat ttgtcacgtc catattcaat
     2641 gcagcaggtt ttttaggcaa accaggcatc gtcataacat ctccagtcaa ggctactaaa
     2701 aatcctgccc ctaattttgg gatgatttcg cggatcgtga tggtgaaatc ttccggtgca
     2761 cctagcagtg aaggatcatc agaaaatgaa tactgtgttt ttgccataca aatcggcagt
     2821 ttgtcccatc cattcttcgt aaactcttct atttgttttt gcgctttttt gctgaaaaca
     2881 acaccatttc ctccgtaaat tttcttgacg attgtttctg tcttttcttg tatcgtttct
     2941 tctaggcggt ataatggctt gtaattagct tcttttctat caatcaatcg tactacagct
     3001 tttgctaaat caacgccgcc ttctgctcca tctgcccata cactcgctct cttggcaaga
     3061 atcccctgat tttcacacaa gtgctccaac agtgtgagtt cattgtctgt atctgttacg
     3121 aattcattga ttgcgacgat cacaggtagc tgatattgtt ccatattacg tatatggcgc
     3181 tttaaatttg caaaacctac tttcaaagca tcaagattct catttttaag ttcatctttt
     3241 ttcattccgc catgcatttt caatgcacgg acagtggcga caatcaccac agcatccggc
     3301 gcttttttca agttaggtac tttgatatct aaaaatttct ctccgccaag atctgcacca
     3361 aatcctgcct ctgtgacaac gtaatctgct aatctcaatg ctgtcttggt agctaaaata
     3421 ctattgcagc catgagcgat attggcaaac ggtcccccgt ggacaaaagc tggtgttccg
     3481 taaattgttt gtacaagatt cggcttgatt gcatctttca gcagtaatgc cagagcgcct
     3541 tctgctttca agtctccagc agtcacaggc tgacggtcaa atgtatagcc gacaacgatc
     3601 cttgcaagac gagcctttaa atcttcaagg tctgccgcaa gacacaagat agccatgatt
     3661 tcgctggcaa ctgtgatatc aaacccatct tctcttggaa caccttggat tggcccaccc
     3721 aagccaacaa ttactttacg cagttcacga tcattgagat cgactacccg tttccaaatc
     3781 actcggcggg catcgatgtt caattcattt ccttgatgaa tatgattatc taaaagagca
     3841 gataatgcat tattagctgt tgtgatagca tgcatgtcac cagtaaaatg caagttgata
     3901 tcttccatag gcaatacctg tgcatatcct ccgcctgcag caccaccttt gattcccatc
     3961 acaggaccaa gtgagggttc tcgcaatgca ataacagatt ttttattgat tttattgagc
     4021 gcgtctccta accctacagt gatcgttgac tttccttcgc cagctggtgt tggattgatg
     4081 gaagtaacta agatcaaatg cccctcagcg ttatcctttg atcgattgat tccagaaaaa
     4141 ttgattttcg ccttatattt cccatacagt tccaaatcgt cttccttaag atctaattta
     4201 ctagccactt caacaatcgg ttctagctct gtttcttgtg aaatttgcag atcattcttc
     4261 atttgattac cccttttcac gaactttatt caacaaaata tcttctttgt taagatatgc
     4321 caattatacc tttttttatt cagaaaaaaa acataaaatt acaggttttt aatttatttg
     4381 acgtacttat aaatactctt tataatgaag gctatgatca gactttagaa aggtcgtgag
     4441 tgctttgacc acaggaattg taaaatggtt tgacaacaaa aaaggatacg gctttatcag
     4501 ttatgatgac acagaagaaa tttttgtaca ttttactgcg attgaagaag agggattcaa
     4561 aactttggaa gaaaatcaag tggtcgaatt tgagatcatc gaagggaatc gaggaacaca
     4621 agctgcccat gtcaagaaag gcaacgttca gactaccgat a
//
LOCUS       DARDHA010000181         4643 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000181 DARDHA010000000
VERSION     DARDHA010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.185"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(232..1305)
                     /locus_tag="SDA10_002547"
     CDS             complement(232..1305)
                     /locus_tag="SDA10_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG4241418.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(1302..2129)
                     /locus_tag="SDA10_002548"
     CDS             complement(1302..2129)
                     /locus_tag="SDA10_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4241419.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(2130..2936)
                     /locus_tag="SDA10_002549"
     CDS             complement(2130..2936)
                     /locus_tag="SDA10_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4241420.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(2948..4033)
                     /locus_tag="SDA10_002550"
     CDS             complement(2948..4033)
                     /locus_tag="SDA10_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4241421.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(4050..4592)
                     /locus_tag="SDA10_002551"
     CDS             complement(4050..4592)
                     /locus_tag="SDA10_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4241422.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
BASE COUNT         1416 a         1009 c          759 g         1459 t
ORIGIN      
        1 tccttctgta actttttcaa tcttagattt actagcccgt ctcgggccct attttacaat
       61 ggttttgtcc acttttctct ggtagaaaaa cgtggtagaa aaaataaaat aacacaaaaa
      121 aagcggcgtt tcatccgctt ttggtagaag attggaaaac tcagatagaa aaaggacgat
      181 ttttagctct ttagaacgtt gataagtccc cattcttact gagttttgcc attatctcct
      241 atacatcttg aattctaaga ataggtcatt atagatgcct aggtatttcg atcctagatc
      301 tttgtagact tcaagatggg agatcgtgtc atcagatgga tagaattgtt cgtccgacga
      361 aatgtctttt ggcaataacg cttttgcttc ttcatttggt gtagaataac caatatactc
      421 tgcattttgt gccgcatttt ccggttctag cataaagttg atgaaatcat aggcaccttc
      481 tttatttttc acggtttttg gcataacgat attatcaaac caaagattgg atccttctgg
      541 tgggatcaca taatgtaaat gttcattttc ccacatcata tcggcagctt cgccagaaaa
      601 tgtcactgcc acggaggctt cttcattgat catatacatt ttgatctcat ctgccacgat
      661 tgctttgaca tttggtgtta aatgattcaa tttatttgct gcttctgtca actgtgccat
      721 attcttacta ttcaaagaat agcctaaact attcaaagaa agccccataa cttctcgtgc
      781 accgtcaatc agcatcaaac tgtcttttaa ttccggcttc cacaaatcat cccaatgctt
      841 aatttgattt gcatcaaaga atttatcgtt atatacgatc cctaaagttc cccaaaaata
      901 tggaatcgaa tactgattat ctggatcaaa atccaagtct aaaaatcgtg gatcaatatt
      961 atttaatcct ttgatcttcg aatgatctaa tggaagtact agtttttctt tgatcatttt
     1021 ttggatcata tattcacttg gtatagcgat gtcgtagttt gtcccacctt gctggatttt
     1081 tgtgaacatc gcttcattag aatcaaatgt ttcataattc actttgtatc catattcttt
     1141 ttcaaatttc gtgatcaaac taggatcgat ataatctccc cagttataga tggtaagtac
     1201 ctttgctcca ctcatgccac ttgttttttc aagttgacga acaccaagca gcagcaccgc
     1261 aataatgatg agaataccta aaaacagaga ttgtaatttt ttcatcgcag ccgtgccacc
     1321 ccctcgcgta ctcttctgtt tttcttcagc cgttttggac ggttttcttt actaataaag
     1381 tagtatccaa tgaccaatat catggagaac aagaaaacta acgcacttag cgcattgatt
     1441 tctaaactga ttccttgtct tgcacgagaa tagatctcta ctgagagcgt cgtaaagcca
     1501 tttcctgtga cgaaaaatgt aaccgcaaaa tcatcaagag aataggtaaa tgccataaaa
     1561 tatccagcta tgattcctgg agaaagataa ggcaaaatga tatttttgac tacttggaaa
     1621 ttattagcac caaggtctct agccgcatcg accattgaat cgttcatttc ctgcaatttt
     1681 ggcaagacca tcaagacgac gattggaatg ctgaaagcaa tatgggaaag caatacactc
     1741 tgaaaaccta atccgaatcc tagtagtgta aagaaaatca agaaactagc accgataata
     1801 acatcaggag agactaataa gatgttattc atgctcaaca acgctgtccg tgcacgtcgt
     1861 tttttcgtgt aatagattcc catagcccca aatgtcccga tgatcgtggc gatcaatgca
     1921 gataagaaag ccagcatgaa cgtattcaat acgataatga tcaaacgtgt atcttcaaaa
     1981 acagcttcat aattttccca agtaaatcct gtaaagctgt tcatcgttcc tccttcattg
     2041 aaggaataaa aaatcagata gaagatcggt gcatataaga ataaaaagac gatccctaaa
     2101 taaagatacg accatttaaa ttttttcatt tatgagcgac ctcctttttt cttctcgcct
     2161 gtcaggagca tcacgatgaa catagcaatg attaaaatca caccgattgt agaacccatc
     2221 ccccagtttt gtgtaaccaa gaaatgctgt tcgatagccg ttcctaaagt gatcacccga
     2281 tttccgccaa tcagacgagt cagcatgaat aaagaaagag aaggaataaa tactgcttgg
     2341 acaccacttt tcacaccatt taatgataac ggaaaaatca ctcgtcggaa ggtctcaaag
     2401 ctattcgcgc ctaaatctct actagcactg atcaaagaag gattcatctc ttccaatgca
     2461 ttgaaaattg gcataatcat aaatggaatc tcgatatacg ttgccacgaa cagaaaactg
     2521 aagtctgtga ataatatctg tttcggcccg atcccgataa aatcaaagaa atgattcacg
     2581 ctgccgtgca cactgaaaat cccaataaat gcgtaagctt taagcagcag attcacccat
     2641 gtaggcaaaa taatcagcat cagccataat tgcttgtgct tcagttttgt taaaaaataa
     2701 gcagtcgggt aactgatgat cagcgtgaaa aacgtgatca aaaatgcata ccatacagaa
     2761 ttgatcgtca tagaaagcga tgtccccgac gtaaaataag atagataatt attcagcgtg
     2821 aattgcccat tcatatcaaa aaaagattga taaatgatca ttaatacagg cgcgatcacg
     2881 aataaaccga gccacaaaat ataagggacg gaatagatcc tgcgcattgt tttcattctt
     2941 gtccccccta ttcttcgtag ctgtcaagac gtgcatcgaa ttcttcttca gactcgttga
     3001 aacgcatgac atggatatct tctggctcaa aggccaatcc tactttgctt ccttctttag
     3061 cttttttcgt tgaatggacc atccattcat tttgctgttc atcataacag atgatttcat
     3121 aatggacacc acgaaacagc tgagtatcga ccgttacgac tagttttcct ttatctggtg
     3181 tcgtgatcgt caaatcctct gggcgtaaaa caacttcaac tggttcattt agacgcatcc
     3241 cgccatcgac acattcaaat tgcttaccaa caaactcaac tagattatct tcgatcatta
     3301 caccgtccac gatattgctc tccccaacaa aatcagcaac gaaatgattg attggctcat
     3361 cataaatatc tacgggtgta ccactttgga cgattttccc tttattcatg acaaaaatct
     3421 catcactcat tgccaacgct tcttcttggt catgagtaac aaaaataaac gtgattccta
     3481 atcgttgttg cagatcccgc agttcatact gcatatctgt acgtagtttc aaatcaagcg
     3541 ccgataacgg ttcatctaat agcaatactt tcggttcatt tacaattgct cgtgcaatcg
     3601 ctacacgctg tctttgacca ccagacattt cactaatctc ccgtttttcg tatccaggaa
     3661 gctggaccat acgcaaagca tcttttacct tcttttcgat atctgatttg gacattttct
     3721 tgatcttcaa tccaaaagca acgttatcaa aaacattcat atgaggaaaa agtgcataat
     3781 cttggaagac tgtatttact tgtcgcttat tagctggcaa atcattcaac cgtttccctt
     3841 caaaatagat gtccccttct gtagcttcgg tgaatccagc tataatacgt aggatcgttg
     3901 ttttaccaca gccggaagga ccaagcagtg tataaaattt tccttgttcg atctcaaaac
     3961 ttactttttt taatactgtt tcatcatcat agcgtttcac tacttgatca aatgaaatga
     4021 ttggtttccc cacgttttta ccccctatat tacaaatatg attgcgttgc aacaatcaat
     4081 acttttgttc gttttttgag atgattcctt aattgatgtt gttttgtcgc ttgataatag
     4141 atcgactgtc ctgcttttgc cacatagacc gtctctccta agcttaactc taattcccct
     4201 tctaatacaa aaataaacgt ttcagataat gaaggttcaa acgtcttata ttctcctgca
     4261 ccttcaaaag tcagcaagac aggctccatt tctttttcat tcgaatcagc cacgagccat
     4321 tccaattcat acccgttttc ttcatcgaaa taaaccgtat gatcttcttt tgaataaacc
     4381 acttgcagat tcgctgcttc ttgatgaaaa aattcttctg gagtcacacc taagacttca
     4441 agaatagaaa agaatgtttc cattgatggt gaacttaaat ctcgttccaa ctgggaaata
     4501 tacccttttg acaaatctgt gcgttcgcct agttcttctt gtgtcaaatt cttttggatg
     4561 cgcaaatttc ttaacttctc accaatttcc ataatttcct ccgaatcgaa gttttctaat
     4621 agtaaacttc ctgtttacta cca
//
LOCUS       DARDHA010000182         4490 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000182 DARDHA010000000
VERSION     DARDHA010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.186"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..219)
                     /locus_tag="SDA10_002552"
     CDS             complement(<1..219)
                     /locus_tag="SDA10_002552"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4241423.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPI"
     gene            complement(330..584)
                     /locus_tag="SDA10_002553"
     CDS             complement(330..584)
                     /locus_tag="SDA10_002553"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4241424.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(777..1052)
                     /locus_tag="SDA10_002554"
     CDS             complement(777..1052)
                     /locus_tag="SDA10_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HEG4241425.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1024..1977)
                     /locus_tag="SDA10_002555"
     CDS             complement(1024..1977)
                     /locus_tag="SDA10_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG4241426.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2589..4082
                     /locus_tag="SDA10_002556"
     CDS             2589..4082
                     /locus_tag="SDA10_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEG4241427.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4217..>4490
                     /locus_tag="SDA10_002557"
     CDS             4217..>4490
                     /locus_tag="SDA10_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEG4241428.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1464 a          750 c          786 g         1490 t
ORIGIN      
        1 gatcggttta ttagaccttt gaggtctata ttttttaact acaatcgacc ttaaatttaa
       61 ttgtttcatt agcttttgga catgtttaag agatactgaa atcccttctt ttaataaaac
      121 ttgatggatc tttgtggcac catagcgttg tttattcgta aaaaaaattg ttgaaatctt
      181 ctttttcaac ttattatttc tgatttctgt agctgtcaac ggtttattct tataaaaata
      241 atagacactt ctagggattt ctaataaccg acacaacaaa gaaacagtgt aatccttaca
      301 ccatttttca ataaattgaa caagcgaatt taggttttcc gagagaatat ggctgcggct
      361 ttttttaaga tttcaagttc ctcatttaaa cgagcatttt cttttctcag ttcagctact
      421 tcttttccag tcagtcctgt ggactgatca ggtaaatata aattcttcca tttgtaaatc
      481 gttgcttcag ataagccata ttctttcgct aaccctcgaa cagaacgtcc agtttgattc
      541 agttcaacaa tcatttgttt aaaattttct tcatatcggg tcataaaaat tcatcctctc
      601 gtagataagt ttaatttatt cttgtctcta cgaaaagctg tctaagattt gatactaaca
      661 ccaaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
      721 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
      781 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
      841 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
      901 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
      961 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     1021 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     1081 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     1141 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     1201 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     1261 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     1321 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     1381 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     1441 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     1501 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     1561 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     1621 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     1681 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     1741 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     1801 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     1861 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     1921 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     1981 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     2041 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     2101 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     2161 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     2221 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     2281 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     2341 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     2401 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     2461 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     2521 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     2581 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     2641 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     2701 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     2761 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     2821 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     2881 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     2941 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     3001 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     3061 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     3121 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     3181 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     3241 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     3301 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     3361 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     3421 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     3481 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     3541 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     3601 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     3661 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     3721 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     3781 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     3841 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     3901 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     3961 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     4021 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     4081 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     4141 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     4201 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     4261 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
     4321 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
     4381 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
     4441 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa
//
LOCUS       DARDHA010000183         4475 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000183 DARDHA010000000
VERSION     DARDHA010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.187"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(43..1686)
                     /locus_tag="SDA10_002558"
     CDS             complement(43..1686)
                     /locus_tag="SDA10_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG4241429.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            complement(1683..2042)
                     /locus_tag="SDA10_002559"
     CDS             complement(1683..2042)
                     /locus_tag="SDA10_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241430.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            complement(2203..2463)
                     /locus_tag="SDA10_002560"
     CDS             complement(2203..2463)
                     /locus_tag="SDA10_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241431.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            complement(2454..2765)
                     /locus_tag="SDA10_002561"
     CDS             complement(2454..2765)
                     /locus_tag="SDA10_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG4241432.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            complement(2766..3338)
                     /locus_tag="SDA10_002562"
     CDS             complement(2766..3338)
                     /locus_tag="SDA10_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241433.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            complement(3671..3931)
                     /locus_tag="SDA10_002563"
     CDS             complement(3671..3931)
                     /locus_tag="SDA10_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241434.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            complement(3921..4109)
                     /locus_tag="SDA10_002564"
     CDS             complement(3921..4109)
                     /locus_tag="SDA10_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241435.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
BASE COUNT         1309 a          959 c          634 g         1573 t
ORIGIN      
        1 tttttcactt ctataactac ccctttcaat aaggagtata ccttaaaatt caatctcatc
       61 caacatatca aatgcttcct cataatccat atcagttatt tcttctcgtt tatataaagc
      121 tgccaaaaaa gcatgaaacc catctgtttt acgccttact ggctcttttt tcaaaaatgt
      181 tttattccct aatttgtctt ctttgataaa cgaattattt gtataccaac gcatcatagg
      241 atcatcacca aaaataaacc gttcatttgc aaaaccatct tcaataatag gagcaacttt
      301 tgactgcaca cctcttatat ttctgataaa ctcaaaatta tatgattctt tctctagaag
      361 aggtttcagc aaatccatac gaaatccgtc ggcacaaacg atttctattt gatggatttt
      421 tcttttttca tttaaccaat caattaaatg acgaggatca attgatggct catcaacgat
      481 tgtcactaaa ccttttttct cccaatcctt aataggcgct ttcagtttga atgtatctag
      541 aaactttttc ctagcaaaac tatgttgctg ccagataaat ttatcatcaa ttttaaaaag
      601 caaacccact gttgcaaaat cacgaatact tgcataatca aatccagcca cacaagaacg
      661 accattcaaa tttggcaatt cttgtttagt agccattaat ttttcatgag tcgtaatatc
      721 tgattctgca tcaccttctg taaaattcat tcgtttaaca acaaattcac gcctacctga
      781 tggctcatct tctagttttt tatattcttt catcactgta gaaaacagac gttttgaata
      841 tgctgaatct tcttcgaaca ttggattagc ctttgaccac attttaggat tatccatttc
      901 atcaatgtta tccagtttac aaataaaggg aaacaagcga tcattcttgt tctctccact
      961 aaaaatcttt tgtgctcgtt ccaacatttt gtcataaaaa ccttctctaa catgaccatt
     1021 tgtaccatta taaaaagttc gaggatgttt tattttccct agcccactac gctgaatatc
     1081 aactaggtct gaagattcaa actgatggat ttcatcaaat tctaagcagc catctcttgc
     1141 actatccata gtcttaggat tgtttgtcct ataactaaaa atcgaattag ttacattgtt
     1201 gactatagcc atcttagtca aataaaattg tttttccagt ttgcgcattt gtaccatttc
     1261 ataaacttct ttaaaagata cctttccctg cttctcagaa ttcgctgtaa tcgttacatc
     1321 ataatttctg acaccgtaga gagggctaat aaaaaatgag tctcttgctg acatgaaccc
     1381 atttttcccg ccgcctcgcg caatactatt tagaatttca tcaaaaaaca cctcgtcgtc
     1441 ctctttttga tagcagaaaa tgaaaggtgt gataaatttt tgatatttag ctaaaggaaa
     1501 aaaatttttt tcagtgtact tgataaactt ttgaatcatt tcttcatcaa aatagatatc
     1561 atcccgaact agcacttcgt tttttaaata agtaattaac tgaattcttt ctttattaaa
     1621 agggattacc cctttttcgt ataaatctac gtattcatca aaatagaatg gctttaatgt
     1681 tgtcacagca aatcactacc atctagcgct gcattttttg taggttcgcg cttttcttct
     1741 ggcaaatagt cagtaagttg cttaattaca cgttgatatg cttggtctct cgaattatac
     1801 tgtttagcaa ctggtcgttc ccgctcgtaa ggtttttgat tttctgactg agagaaaagc
     1861 tcataatcac ctttttcgga aatatcaatc cacatttcat caagaagaat ttttaagcgt
     1921 gccgcttgcg taatcaaacc agaaacgacc ttctttttat ccttatcaat atccttaaac
     1981 aatttattta gacgcctttc ttcagcagaa acaagcctat ttctctcttg cagatcagcc
     2041 atcaactcac ttcctttcta cgtggggagg gggtcgtgtg aaaatagcat catttatttg
     2101 cagagttgac cccatccacc ggttcccaaa aattttattt cttctcaaaa tcttttgacc
     2161 gggggtattt cactctatat atcttcgctt tatactgatt tatcagccgt cttgctcgtt
     2221 gctttctaca atatgtgcaa atggactcaa ggtataatca atgtctacat taatattaaa
     2281 tgtccctgtt acttcattag cgttacctat tagtctattg ttgatagata tatcctctac
     2341 agttgttgtt ccttcaatga gctgactctt aaatagaacc aacttatcaa tagccttatc
     2401 aaccatagcg attgcttctg ctcgtttgac atcatctctg tttgctgttt ttcttaccac
     2461 cattcatcat cccactttct tttcttttgt tggccacgat atttcatacg gttgtgcctt
     2521 ttgttatggc aatctttgca taaggttctt aggttgttta tatctagagc taaatccgga
     2581 tgatactcca gctcctttat atgatccact tcaagaattg aatcatgttg agttgttagt
     2641 ctaccctctg ctttacacca ttggcactca taatgatctc gctctaagca ttgacttcta
     2701 agtttcctcc actcagttga tccatagaac ctagctcttg cttgtttact tgaaacatca
     2761 atcatttaca tatctctctt gtgcccaatg atttttctat ctaagtactt gttattatgt
     2821 gaatcataat attcaatagt gatgtcgtta gctccttttg gtacttggca ttcatattct
     2881 tccgtatccc aatgataagt aatatcgaca agacctatcg gcatctcatc cattcgttga
     2941 cctttataat aaacttctgg tactgagtca gtatctctta gtttgatttc tagaaggttc
     3001 gtattacttc catcatctaa cgtcaattca gcaatcctct ttttgatctg ttgaattact
     3061 tttcttcctg tctcacctgt cattggtact accgtctcat atccagtaaa ctcgcaagta
     3121 aagcgttcaa tagcataagc gttttgaagt ggcttagcac taagaacagc tggattattg
     3181 ctataaaaac gatctactaa taacttccct ataggaatgg catctaaagc tctatcagta
     3241 aacactaaag cattgggata atcttcttga attttgtaag ctagattggg tgcagttaca
     3301 acgttatacc cttctctgat ataatctttt aatttcatag ttttcctcct aaaaacatct
     3361 aataattact tgctaacaac ttgatgtaat catagttatc attcatttga tgtacctctc
     3421 aatgttgtat tggatatact cgtctttcca atagccatga ccgcaatata tcagcttgca
     3481 tttatccact tcgtttggtg tagcttctct tagcatttcg acaatagagt acttcccttt
     3541 gatttgtaca gaacgcacaa cacgcactga acaatcacca atggttcgag gatattcatt
     3601 agttagtgat acataccagt agtttctcat tatgcagcct cctttatgta aagaaaaagc
     3661 ctagcacctg ctagactttg atacattcat ttgatttttt gagataagta tctacataga
     3721 tttcaccttt gtccccgttc aacgtaattt caaaatacgg tgctcctttt aagctagcgc
     3781 tgactaatgc tttgctattt tgcagtgttt tgcatgacca gactatgaaa atactaaact
     3841 caggcactgc tccacttagg tcagcaatct cattttcaat tctttcacgt acaatagcct
     3901 tgcatttttc gataaacttc tcatgattca ttttgtacct ccgcaagcgc ataaccagct
     3961 gttgatgtat tgttaaacgt agctgaacga gtaactcccg tagccttccc agtataaata
     4021 aaactaaatc ctgttgttgt tggcttaaaa ttctccactt cataaaaatg atatgtttgt
     4081 ccgttattag taaatacaat caattccatt tttcaaaatc ctctcttttt taaaataaaa
     4141 agaccactca aagagtgatc taatatgtaa tgcacaggca gggacgtttc cgatcctgtg
     4201 cttgagtcat ttgacgattc atttgtaccg aatcccaaaa ctcaatctaa cccaaccaat
     4261 tatgtaatag caacctacaa gatgcacaaa acgcgtacga aattgcgcac ccctatattt
     4321 ttaaaccgcc gatgcctcgg ttaccaaagt cactggcaag gaatcgaacc ttgcagtttg
     4381 cacctaaatt ttagatgagc gatacacctt tccaccacag tgacataatg acaatagaca
     4441 gcaactgatg atagataata agaacaattt agaag
//
LOCUS       DARDHA010000184         4467 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000184 DARDHA010000000
VERSION     DARDHA010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.188"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..930)
                     /locus_tag="SDA10_002565"
     CDS             complement(<1..930)
                     /locus_tag="SDA10_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose 1,6-diphosphate aldolase"
                     /protein_id="HEG4241436.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELS"
     gene            complement(927..1385)
                     /locus_tag="SDA10_002566"
     CDS             complement(927..1385)
                     /locus_tag="SDA10_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4241437.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(1382..2845)
                     /locus_tag="SDA10_002567"
     CDS             complement(1382..2845)
                     /locus_tag="SDA10_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4241438.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(2827..3759)
                     /gene="pfkB"
                     /locus_tag="SDA10_002568"
     CDS             complement(2827..3759)
                     /gene="pfkB"
                     /locus_tag="SDA10_002568"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEG4241439.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            3901..>4467
                     /locus_tag="SDA10_002569"
     CDS             3901..>4467
                     /locus_tag="SDA10_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG4241440.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQ"
BASE COUNT         1338 a         1011 c          717 g         1401 t
ORIGIN      
        1 acttaattct ttaatatttt gttttccttc ttctaacatc caactactcg ctttttcttc
       61 gccttcggta acaaaaggtg ctactccctt cgcccatgtt gctcgcccac ataagacacc
      121 attgaatgtc gaacctgatt gttttgcaaa acgcaatgta tcttgaaata gtgcagcgct
      181 gactcccgca cttaaaaaaa taaacggttg ttcagtcact ttcgattgtt catagaaata
      241 cgcggatgcc tctgcttgac tataacaagt atcttgccca tatccttcca caaatctcat
      301 attcactggt acttctactt tcaatacatc cacttgataa cgttcttttg aaaattctcg
      361 catggcttca atcactttat gcggttttac ttttgcatag tcaagagaag acggatcgcc
      421 aatagttgca tcgtaagaaa ggagctctaa aaagaaaggt atgccttctg cttcgcattc
      481 tgaaccaatc cgttctataa ctatttgttt ttgcacatta atctctactg gttcatcaac
      541 gtcatagtac aataaaaatt tacaagcatc cgcacccact tcttttaacc ttttcactga
      601 ccaactattc agtaaatctg gtagtcggcc tggcattttc gcatcatagc cggttttttc
      661 ataagctagc aacaaaccac attctttctc tcgaagcgct gctgcaggca gaccatattc
      721 tggatcgagt aaaatagcgg aagcatacgg agtcaggtac tgagaaacca tctgtttaaa
      781 ttggatgata tcttctgatg tagcttcttt atattggttg atcattttct tcaaagcacc
      841 acgttggtcg atagctagcg cgctaataat tccagcttca tccgtcaatc gcttcaaacg
      901 ttcctgtttt tctatactca ttttcttcat atatcttcac ctatttcttt agttaatatt
      961 gtagctattt ctatttttga ttttgcagct ctcaactgtt ctgtaacctc ttttcgcatc
     1021 aatgatcgtg caacgagaga caataatttc aaatgagtcg ttcctgcttc tgaatctggg
     1081 ataaataacg caaaaataaa atcaactgac tgtctatcta aactgttcca ttcaattcct
     1141 tttttcagtg tgacgataat caaactaggt tcattgattg tttgagactt cgcatgaggg
     1201 atagcaaaac ctgacattat tcctgtagta ccttcttttt ctctttccag aagtttttga
     1261 tatacttctt tttcgtcatg agcgattcct aaacagaatg tttgctttgc taaaaactta
     1321 aatgcttctt cttgattatc taatggctgt tgtaaaaaaa tgttctcttc atgaatgatt
     1381 gtcatgataa acctcctgct cttccttctt cgataactgt acgatttgtt gctttattat
     1441 ccctcttctt gtctttatca gttactcttt tcttttggat caaacctagt atcactccac
     1501 caacgatcga accaataaga atcgctgtaa cccattggat ttttcccgtc actacaggta
     1561 acactaaaaa accgccatgt ggggctggta cttggacacc aaacagatag gtcaagacag
     1621 cagcaattga tgagcctaac atcatcgttg gtaatactcg gatcgggtct ttcgcagcaa
     1681 acggaatagc tccttccgta atatgagttg atcctaaaat aaaattaacc aaacctgcat
     1741 tacgatcatt agtatcaaaa tatttaccaa acaataaagt agcaaaacca gtaatcaacg
     1801 gtggtgcaat gcaagctgct gacgcaccag ccataaaagt cgtgtttcct tgtgtcaaaa
     1861 gagctgttcc tgttacatat gccgccttat taaccggtcc tcccatatca aacgcacaca
     1921 tacagccaac aatcacgcca agaacaagtg gattcgaata ttcaaatcca gctaaaaagc
     1981 tcatcattcc ttcatttata gccttcattg gacctgccag caaagacatc acgatgccaa
     2041 taaatgctac accaataact ggatataaga cgattgcctt caagccatct agttgtttag
     2101 gtaaaattat cagtagcttt tgcaaaaata agataaaata acctgctaag aacccagcta
     2161 aaatacctcc taaaaatcca gtaccgccat tcatggctac gattccacca acaaatccaa
     2221 cgactaaccc tggacgtttc gcgatccctt ctgcaatata agctgctaaa actggaacca
     2281 tcattcccat tgctgcatcg tcaactgctt ttaccattgc cgctgtagca ttgtattgtg
     2341 catgatctgg atcagaagaa tagattcccc aaacggcaaa tgaaatagca atcaatactc
     2401 cgccagctac cacgaaaggc aacatatgag agacaccgtt catcaaattt ttatagatgc
     2461 tacgaccaaa attagcacca cttatcgctt ctggttctaa agtatcagca gcttcattat
     2521 ctgctaagac agtacctttt cctgccaatg cttcgtcaat cagccgttgt gcatccttga
     2581 ttcctgtact gacagaaaca tcaatgactc gttttcccac aaatctttct gaatggacat
     2641 ctttgtctgc ggcaataatg acagcatctg cctgtttgat ttcttcagcc gttaattcat
     2701 tctcgatccc aatttgtcca tgggtctcca ctttgatcgt gatgcctttt ttcctagctg
     2761 cttgctctaa tgcctcttgc gccatgtagg tgtgcgcaat tcctgtcgga caaccggttg
     2821 ctgcaattag ttgatatttt cccatctttc gaacctctct tccttgattt tgatttgttt
     2881 acttaattcc tccacatcca aaaaatcagt cagtccttta cgaaaagcag tagagctgcc
     2941 tgctgctatg cttttacgta acgtttcatc aagcgatcgt cgattcatgt atcctgctaa
     3001 aaaagtgcct aacatggcat cacctgcaca tgctgtattg acaacttttc cttttggcga
     3061 gtttcctcga atcaccctgt ctttcgtaaa aagaatcgct cctttctcac cgagggataa
     3121 caaaacattt tctgcgcctg actctactag tcgttgtcca taaacaaagt aatcttcatc
     3181 tgtaaccatt tttcgtccga accaagaaga aagttcttcc tcatttggtt tcagcaaaaa
     3241 cggtcgatag ggtaaacaat ccatgacttc ttgatcacta ctatcaataa tcaaacaaat
     3301 ccgactgatt tctattaaaa cctttggaga cagccctctt ggtaaactcc cagaaacaca
     3361 aagataatcc cctgcttgta atgctcttat ttgatttaaa aaattatgaa cagctgtctg
     3421 cggtatttca ggtccttgat tcactaattt gtattcttct cccgtttgat tgacttgtgt
     3481 aaatacattg atacgcgtca taccaggcac ttcaataaac tctgtttgga tttgtttctg
     3541 ctgtaaatag tcttctatat actttcctgt gaacccagca gaaaaaccta atgccgtact
     3601 tgggaaatct agcatcttta atactagtga cacattcacc ccttttccgt ttgcctgaat
     3661 gtcatcctct tttgtgcggt ttaccataaa aggatgcatc tcatctgttt caataaatag
     3721 atcaattgcc aaattcatcg tgcatgtgta gatagacata accaacgacc tccttgattc
     3781 tattatgaaa tacgcgaaat agaaaatgtt ttcttaatcg tcccaacaca aagaaaacct
     3841 ttacatttat aaactcgtcg tgcttttttt aactaacttt tggtgaaaga aggaaagaat
     3901 atgaatcaca aattaagtga aacagagcag tacctctgga actttatcga acacctcatc
     3961 ctagagatcc ctaactattc catcgtgaaa ctaagtgaac aagcaaatgt ctcaacagcc
     4021 accattgtgc ggacaatgaa aaagaaaggc tatgaagggt ttacttcgtt taagcatcat
     4081 ctaaaagaag gagaaaacaa gaacatcaat ttttcatttt tagataaagt agacaaagga
     4141 atccgccgag ccattctaaa aaatgaacaa gaagtggtac gtacaatcaa tatgttggaa
     4201 atcgggaaca ttgaagatgc aatacaaaga atcaaatttg ctgaccgggt ttttgtcttt
     4261 gcccgaggat tttcagaaat gattggccaa gaaatgctgg taaaacttca attaacggga
     4321 aaaaattgtc agatgcatac tgatccagaa attatcaaaa gcatcagtcg gaaactaact
     4381 aagaaagaca gcgttatttt tgtttctttg aacggtgaga aaacaaataa aaacatcaaa
     4441 aataagattt atgtctttat ttttcaa
//
LOCUS       DARDHA010000185         4434 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000185 DARDHA010000000
VERSION     DARDHA010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.189"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(543..2048)
                     /locus_tag="SDA10_002570"
     CDS             complement(543..2048)
                     /locus_tag="SDA10_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG4241441.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="SDA10_002571"
     CDS             complement(2045..2731)
                     /locus_tag="SDA10_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4241442.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="SDA10_002572"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="SDA10_002572"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEG4241443.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1547 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DARDHA010000186         4425 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000186 DARDHA010000000
VERSION     DARDHA010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.190"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            690..1658
                     /locus_tag="SDA10_002573"
     CDS             690..1658
                     /locus_tag="SDA10_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG4241444.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="SDA10_002574"
     CDS             1669..2484
                     /locus_tag="SDA10_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HEG4241445.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="SDA10_002575"
     CDS             2552..3262
                     /locus_tag="SDA10_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4241446.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="SDA10_002576"
     CDS             3298..>4425
                     /locus_tag="SDA10_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEG4241447.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DARDHA010000187         4380 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000187 DARDHA010000000
VERSION     DARDHA010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.191"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            228..959
                     /locus_tag="SDA10_002577"
     CDS             228..959
                     /locus_tag="SDA10_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG4241448.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1104..3131
                     /locus_tag="SDA10_002578"
     CDS             1104..3131
                     /locus_tag="SDA10_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEG4241449.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3239..4336
                     /locus_tag="SDA10_002579"
     CDS             3239..4336
                     /locus_tag="SDA10_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEG4241450.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1537 a          710 c          907 g         1226 t
ORIGIN      
        1 cgaattattt taaaggctat gacagctgta tttctagatt gtgtttgcat caagttgcaa
       61 taaaaactag aaatcaaatc gggctgataa tcatttataa ctaatcgata cataggacca
      121 aatataggtc tatgttcttt tatttcataa gtatcgtaaa acagaatttt gctatggatt
      181 aatccaatcg actgatatgt ttagtaaata aagaaagagg aatgtcaatg atgaaaaaag
      241 aaatcgtatt aacagcagca atgatgttat tcagcatggt agcaagtacg acttttgttt
      301 ctgcaactga agtataccca aaggaatata acacagaagg aacgattaca tttgaagcag
      361 gagatgaggg tgtaactcct cctgttgatc cagagaatcc tgatcccaat aaacctgtcg
      421 atccatcaga tcctccaagc cctggaacag gaggggcatt atcgattgat tatggatcaa
      481 aattcaaatt tgggactcaa aaaatttcta ctgcggataa aacgtattat gcagctgctg
      541 atgtgatgaa cgatgggtct agaaaaccga catatgtcca agtgacagat aggagaagta
      601 ccttatctgg gtggaaatta agtgtctctc aaccagaaca gtttaaaact gccagtgggg
      661 atgaactagt tggagcacag ctgaaattta cgaaagggca ggctgtttct ttggttgatc
      721 caacatatac gccgcaaacg gtgaattctg aattaacgtt gacaccggga ggaaacaata
      781 cgttagcaat taatgctaaa tcaggaacag gtgtggggac ttgggtctat cgatttggtg
      841 caaatgaaaa tgaaaatcaa gatgcggtac agctttcggt acccggtaaa tctgtcaagt
      901 tagcgcaaca atattccact aaattagttt ggacattaga agatacacct aacaactagt
      961 ttactgaaaa gaactgttaa aaataaatac gtagtcatat tgtcttgcga ggtaatcgag
     1021 caaaaattgt caaaggctag gctaaatttg tttggatacg aatagttcat ctaggaattt
     1081 aacgatcagg aggaaaagat atattgaaaa agaggaggat cgtgctcatt ttaagcagtc
     1141 tcttagtgac taatatcttt ccgatcatag ggtactcttt ggaattgaat cagttggagc
     1201 atggaaatag aagagacgaa gaaagcaaac tcacttcgac attcgacagt ttaatagaat
     1261 ccaaccagac acaagaaagc agtgaggatt taagtatttc gagtagtgga gagaccactg
     1321 acaacgtaga agctgagggt tcggacaaac aaggactaga agatgcacca atttctgcgg
     1381 ttgagctgcc tgacgatccc aatattattt ctatcgataa aatttttcaa acacctatcg
     1441 gtactagcac gtccatattg gaaaatggga agttactgca gttgaaccca gcagaaaggt
     1501 ctcaaagagg agctatttgg tcaaataaga agattagttt actctcggat tttacattta
     1561 aatcttattt atacttagga aatcaacgag atagagcggg agatggcatg acctttacat
     1621 tgactggcga tcctagaatg gctagtaatc ccagtcaagt tattggatcg ccaggtatgg
     1681 gaattggctc atattctact cgttctggcc aaccttacat tgcgaatgcg ctctcgattg
     1741 agtttgatac gtataaaaat agtggttctt ctgatcgaat ggatcgagag ataactgctg
     1801 ataatagagg gcatggacat gtcgcttttg tcacgcctaa accaaataat aataattatt
     1861 ctggagagca tactggcgtg acggtagctc ctacatttct ttctaatggc acatggcgct
     1921 tgttaataat taaatgggac gcggagcaaa gaagtcttac ttatgatttg gaaggtgtcg
     1981 gtcactcttc tcggattata cctgatctca ttaggcagtt tggttctaca gaagtaaatt
     2041 gggggtttac ttcttcaaca ggtacttttt ttcaagaaaa tgctgtcgct gtcacgcgac
     2101 ttccatcgaa tgtcacatca acagctgatg taaaagtcaa cgatagtgat tatggggcca
     2161 tcgctgaagc tggaggaaac gataagataa ctatcagaaa tacattgaag gtcgaggggg
     2221 attttcttga atataagaaa atgcagacta gtatcgacct cccacaagaa ttaactattc
     2281 ccgagcatat cttgatcaat ggaaatcaaa ttgatgccaa agatatcttg ataaccggaa
     2341 atcgagtcac gctaaatctg acagattatt ttattgctgg agatacgtta ataatcgaat
     2401 tggagacaac tttgcaggaa gtaattcctg aaaaagtact cactatgcat tttgaatacc
     2461 ttgaaaacgg gaatttgatt gagcaatcga atgaaataaa cctaaagatc gcaaagctga
     2521 aggagataga tatccatgtt tattataaag ataaagacag tcagattgat attgctgaaa
     2581 tgaaaacaat cactgggaaa ataggagaga agtataaaga aactcctatt gatattgaag
     2641 ggtatgtttt tgactcggat tcagcgaata cagaaggcat cttctccgaa aatacagagg
     2701 atatctattt ttattacaga aaaggcaaac tttatctctc aactgctcct caatatgtca
     2761 attttggaaa acataaaata tccagtagac cattaagtga attcggacag ttttcaggtg
     2821 ggttgaaaat tatagatgaa cgggcttttg gaagttggaa attacaattg aaacaagaga
     2881 aagttcttac aaatggagat tttgagcttc cagaagctct ttcctttgtt acctctgaaa
     2941 atacaacggt tatagggaca tccgctgtta cgattttcga aagtgatcaa aagggtgaaa
     3001 gcgatttgtc ctctctttta gattctacag ctcatcgtgg aatcagaata gatattcctg
     3061 tagaaaacca aagaatcgga acctttgagg ggaaactatc ttggatactt gcagatgtcc
     3121 caggaaatta acaggaagag aagaatataa caaaattatt gagtttcaag aagggcacag
     3181 gtaccatagg ctcttgtgtc ccacagagac aagattttgt tatagtcttc tcttacatat
     3241 gattaaagga gcgattttct tgagaccaaa acaacaatta tttataacac tcctagttat
     3301 tttaggggca ctttttaaca atatatcagt gtcagctagt gcatttaatt tttcagtgac
     3361 gccgataact tcagagaatc aaatcgacaa gagaaaaact tacttcgatc ttcaacttga
     3421 tccggatcag gaagtagaag taaaagcgga attgagaaat gatacagaaa aagaagtgaa
     3481 aatcgatatt tctgtgaata gcgcaaccac caattcaaat gtgatggttg aatacggaaa
     3541 aaacgagatt gaaaaagaca tgagcctgat ctttgatctg gtagattatg tgagctatcc
     3601 acagatagtg acgctaaaac ctaaaagtgt ccaaaccgtt gcttttcatg tcaggatgcc
     3661 gaatgagcga tttgatggtg tattggctgg tgggatcaca ttccaagaaa tccagacgga
     3721 aaaggaccaa acagaaacta aggatcaaag cttatcgatt gagaatgaat acacctatat
     3781 tattgctttg ttgatgcaac agacactgaa cgaagttgct ccgaacctta ctcttcatga
     3841 agttaaacca gatcaaatca atgcaagaaa tgtcatctta gcaaacgttc aaaatgatca
     3901 aaaaacctat atcaatcaag tagttattga aacaaaaatt acaaaaaaag gacattctga
     3961 agttttatat caagaggaaa aagaaggatt gcagattgcg ccgaatacaa atttttcttt
     4021 tccaactgct ttaaatggtc aaccgctaac cccaggtgaa tatcacctta caatgactgt
     4081 tttaggaaat gaaaatgaaa atggaaaatt ttcgagaaaa aaaggaaata ctacgatcaa
     4141 ctataccaat caatgggtat ttgaaaaaga ttttacaata gacggaaaag tagccaagga
     4201 attgaacacg acggatgtta cgattaaaaa agacaataat tggatttata tcttagtagc
     4261 ttcacttttc ttattgttta ttttattatg catacttttg tttgttttga gaaaaaaaga
     4321 agagaaagaa aagtaaggtt aagataacaa atagtaaatt tttattattg taggatggtt
//
LOCUS       DARDHA010000188         4345 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000188 DARDHA010000000
VERSION     DARDHA010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.192"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(387..1853)
                     /locus_tag="SDA10_002580"
     CDS             complement(387..1853)
                     /locus_tag="SDA10_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4241451.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="SDA10_002581"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="SDA10_002581"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEG4241452.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1412 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaa
//
LOCUS       DARDHA010000189         4324 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000189 DARDHA010000000
VERSION     DARDHA010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.193"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..281)
                     /locus_tag="SDA10_002582"
     CDS             complement(<1..281)
                     /locus_tag="SDA10_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEG4241453.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
     gene            complement(730..2138)
                     /locus_tag="SDA10_002583"
                     /pseudo
     CDS             complement(730..2138)
                     /locus_tag="SDA10_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2359..2721)
                     /locus_tag="SDA10_002584"
     CDS             complement(2359..2721)
                     /locus_tag="SDA10_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241454.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2725..3063)
                     /locus_tag="SDA10_002585"
     CDS             complement(2725..3063)
                     /locus_tag="SDA10_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241455.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3042..3461)
                     /locus_tag="SDA10_002586"
     CDS             complement(3042..3461)
                     /locus_tag="SDA10_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241456.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3454..3687)
                     /locus_tag="SDA10_002587"
     CDS             complement(3454..3687)
                     /locus_tag="SDA10_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241457.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3684..>4324)
                     /locus_tag="SDA10_002588"
     CDS             complement(3684..>4324)
                     /locus_tag="SDA10_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG4241458.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1223 a          961 c          620 g         1520 t
ORIGIN      
        1 atattctctt catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa
       61 ccattacctt catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga
      121 gtgtgataat aaatattttc tttcagtttt ccgactatgg taattcggtc aattgaccac
      181 tgcattttta agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt
      241 aatttttccc actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag
      301 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
      361 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
      421 agctagtgcg attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt
      481 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
      541 atgcaaatat ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
      601 acgctaggtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt
      661 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
      721 acatcgtaac taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt
      781 ttgacgataa aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat
      841 atccttttct gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt
      901 ttttaatcgc tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc
      961 cacttgtaaa tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc
     1021 tacatactca tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt
     1081 atcagcattt ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc
     1141 attcccagat aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat
     1201 atctttttgg aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc
     1261 ttcaatcttc actctctcag aagccaccag agccttttct cgctcatttt cttctctttg
     1321 cctagaaaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac ggccgtttga
     1381 cgcaattctg aaatctcgct catgatttct ttatattcgt gaacatcttt gattttgtta
     1441 gtaccgacta actcacgctc ccaaccaaaa tctgccatca aacgttcaat tgactcaact
     1501 tcctgattcc taaaattatt ccaaatcact cgaccattct ctttatctgc tcgtaaaccc
     1561 tgctgttgca aggctttgtt aaaacttggt tgcttttgaa caccccgttt atacccttca
     1621 gcaacaggaa caatattcaa atgcaggtgc ggacttactt cgtcattatg aataacagca
     1681 ttgtagacgg ataaattagg attccgttct ttaaattctt tgaaatattc aactaaaatt
     1741 ttgttggcaa tcactcgatt ttgttctgct tgttgttccg atatgccagt agaagaacca
     1801 tcatcacgag tagtacgaaa atcatcaaga gtaccaattt gcacgataaa ttctcgctgt
     1861 ggttccaatt ttttatcctt gcgaaccttt gctaaatagt cctcgatttt tctatcggct
     1921 cttttctgct ttgcattata tttttctacg gcttcaccga ataattcttc atatttttca
     1981 cgaatatttg tttgttctaa atagatattt tcatccgatt ttgtgaaatc aatatgctta
     2041 tgccagtcat tgattttttg gctatcagtt aactcacgat tattatgaga aatgcttgta
     2101 ttctttgttc cctttttgaa cgaaatactg aaccccaagt taaaaccctc cttcggctgt
     2161 cgctaataaa aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta
     2221 attgtgacat ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga
     2281 caacgaaaaa aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc
     2341 ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact
     2401 ctcactcatc tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg
     2461 ataatctcgt tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt
     2521 ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc
     2581 aaacccaaaa cgcttccgaa gagccttgcc cattgcagca ttttctttct caatcaagcg
     2641 ttttttctgt tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat
     2701 atctgctaaa gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat
     2761 ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc
     2821 agttgtttgc gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctaa
     2881 atcagcattg ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt
     2941 atcttcatcc ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc
     3001 gactgtttct tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac
     3061 cattttcgat tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga
     3121 agcagggtca atttttgttg atcacgaatc atttctgtta attcagaaat atgtttgtcc
     3181 ttctcagcta attgatcacg caaaaactca ttctcttttt ctaaaggaaa atcactgcct
     3241 gtgttattga ccggtaattg accagtcaat tgatcacttt ttgaccgata aattgaccga
     3301 acaacaccaa tcaactcttt atctagcatg ataatgccat tttctttata ctgagcaagg
     3361 tcaaaccctg aattgaccag ttgattgcgc actgtatttt tcgatacacc caattcgtca
     3421 gcaagatttt ttatggtcat cttttctgac aaatcagtca ttagcttctc cactactttc
     3481 agctaccgca taaatatatt taacggcgtg cttagggata ataatcggag agtcaatttt
     3541 attatttttc aaaatcaatt catattgcgt tacatcaagt aacttaccgg acattttttt
     3601 gctatccaaa aaaacaacca caacgtaatc acctgttaaa tttaccaacc aatttttctt
     3661 accaaaatac ttttgttttt ctgtcatttt tcttcctcct ctgttacgtc tagccctaga
     3721 agccatgtgt ctaattcttt ttttaaatcg tctgccgatt ccgcaaaggt ttctcgcaaa
     3781 tgattcgtca attcatcatt ttgactttct tgcttcaaca aatccctgta ttttttcttt
     3841 gtattcttag ctagatttcc ccactcagaa tgatcataaa gcaaaccttt caccataatc
     3901 cgttcagtaa ctttagtatt tggcggaaaa taatgtggac tagcaaagct atccaaactt
     3961 tcatgaacca tctcatgcca atcagttgct tttccccttc gtaactgcaa ttctagtcgc
     4021 caccatgtcg ttatttccgg cggaacaatt ttacgttttc tttcttgttc cagtttttta
     4081 ttatacatcc gaatttgtcg ttcactggca cgactacccc aatatgccgt ttctaactta
     4141 ccagaacgcc cataaattgg tttaaaagac acaggatcaa ctacccgata ttgtgtaata
     4201 aactcatcag gtacatcgat aatgtcgcaa gcaatatccg cacgtgaaaa gtgaggttcg
     4261 ataaacaaat catgaataaa atttttcatg gaactagcta agaattgact aatcttattc
     4321 ggat
//
LOCUS       DARDHA010000190         4310 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000190 DARDHA010000000
VERSION     DARDHA010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.194"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(65..1285)
                     /locus_tag="SDA10_002589"
     CDS             complement(65..1285)
                     /locus_tag="SDA10_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEG4241459.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1916)
                     /locus_tag="SDA10_002590"
     CDS             complement(1305..1916)
                     /locus_tag="SDA10_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG4241460.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="SDA10_002591"
     CDS             complement(1959..2891)
                     /locus_tag="SDA10_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEG4241461.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3312..4172)
                     /locus_tag="SDA10_002592"
     CDS             complement(3312..4172)
                     /locus_tag="SDA10_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4241462.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1230 a          866 c          707 g         1507 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
     2941 atttttgtta ctgttttcac aaccagatga tacgcctgct agatttattt gtcaaccgat
     3001 tttatatatt tttttatata ataaggtaat tttaatataa aattttatat tgtaaaaaaa
     3061 caaataaggg aaacaaacaa cacatttttg tgtgttgctt atttccctct aactatcgtt
     3121 ttctatttga tggttaacct acttaaaata aatactgcaa cgacaaacgg agatcaagca
     3181 tgctttgtaa cgtattttac gataattacg actttatttt attactctat cttgctgcca
     3241 tacccgtcta tccctaatca tctttggtga attttttcaa agttagcata acaaaaataa
     3301 gtattttttc cttagttaat caatagtatc atttttttca tatgatttaa ttcatcaatt
     3361 aattttttgt ttttattaat tttagcaaaa gctagtgcat cttgaatcaa ttcgagcact
     3421 tctgccttcg acttattttt agcctgtgcg ttcttcgcta attgggcagc agctcgtgct
     3481 aagtaataag tcaaatggtt atccgaacaa atatcgattg cgtaatttag tagtgcatca
     3541 cttgtttcca aatcaccgtc ttgcgcaaaa aaaacaccac aatgaaagac gatatttaac
     3601 acccgccaga tatcttctgt tgtacgaatc ggatacgtat aaattttttc aaacactcga
     3661 ttgaaataaa cttctgcttt ttccaactct ttttctctag catagaccat tccaatacct
     3721 gtatatgcca ataaacgaaa caacgtgttg gctgtttctg ccatcaacaa ctgatcaaaa
     3781 tggaacaaca catccgtaat tggtgcattc gtaaaaatca tttcaaatcc ttttagatag
     3841 tagtaacgtg caatcagctc agactcgtca attaccttgg gatcaatggc tgctaaaatc
     3901 tgtgtcaatt gttcatattc actcgtaatc agtgaaaact ctgctttttc tagctgttca
     3961 tcaagttgtg tattgatcac gttcatcgaa gaaaaaagtt caactaacgg aagattgatt
     4021 cgattacata atttgattaa aatttttaac gaagggactt gaccattgtt ctcaaattta
     4081 cttaatgttg cttgtgtaca aattccttca gacacttctt tttgagataa gttggcagcc
     4141 tttcttgccg taataaattt ttcaatgttc attattttct ctccaatcag acctcacacg
     4201 aaaatcattt ctgtctcgat tttagcatgt tttctttggg aatgctttag gttctgttgc
     4261 aaagttttaa ataaagaata aagtccctta cggtatctat gatttaagct
//
LOCUS       DARDHA010000191         4287 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000191 DARDHA010000000
VERSION     DARDHA010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.195"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..312
                     /locus_tag="SDA10_002593"
     CDS             <1..312
                     /locus_tag="SDA10_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241463.1"
                     /translation="SNKLTIELDNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKF
                     GTTELTIPPGINKVVITYQYTKLAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHS
                     H"
     gene            complement(457..1197)
                     /gene="truA"
                     /locus_tag="SDA10_002594"
     CDS             complement(457..1197)
                     /gene="truA"
                     /locus_tag="SDA10_002594"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEG4241464.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(1388..2023)
                     /locus_tag="SDA10_002595"
     CDS             complement(1388..2023)
                     /locus_tag="SDA10_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEG4241465.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(2170..3411)
                     /gene="sstT"
                     /locus_tag="SDA10_002596"
     CDS             complement(2170..3411)
                     /gene="sstT"
                     /locus_tag="SDA10_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEG4241466.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            3609..>4287
                     /locus_tag="SDA10_002597"
     CDS             3609..>4287
                     /locus_tag="SDA10_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241467.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISL"
BASE COUNT         1428 a          855 c          653 g         1351 t
ORIGIN      
        1 agtaacaaac ttacaattga attagataat tcgaataata aaaatactgt cttgacgaca
       61 cctatttatc gttataaagg gcaagtggct tctattaatg gcaaactagt acaaacaaaa
      121 ttatctaagt ttggcacaac tgaattaact attcctccag gtataaataa agtcgttatt
      181 acttatcagt acacaaaact agcaattgct tcaagatatt tatctatcgt tactttgatt
      241 ttatttttac tttatcgatt tactttttct aaacaaaaac aaccgagaag agaagtacaa
      301 cattctcact agatttttat ttaagaaaga ctttttagct gtacacataa acaggctaaa
      361 aagtcttttt ttatatttct ttaatttgct tcatacatta tttaacatcg atatttatac
      421 tgtgaaacat gcgtttcatc cattatcaaa gtatttttat cgataataaa cttcatccaa
      481 aaacagacct tgtgcaggga cggtttctcc tgcatgttgc cgcactttgc tttcaaatat
      541 ttcatcgatt gtttctatcg gcatggcacc tgtgccgatt tctagtaatg tccccatgat
      601 aatccgcacc attttatata agaatccgtc tccgacaaaa gtaaaatgaa gcatttctcc
      661 ctttcgttcg atagataatt catcaatcgt acgcactgtg gatttttttg attttttcaa
      721 tgcagaaaaa ccaatgaaat catgtgtacc cactaatttt tcgcaagctg cattcagctt
      781 gtccatatcc aactcttgag ggaaataaaa gctgtggttt cgctcaaatg ctgaaggaat
      841 cacgttgttc caaacataat agctgtattt tttgcccacc gcattatatc ttgaatggaa
      901 gcgctcaggc atttcttcga ttcttttgat gacaatatcc cgcggaaggt aacgattcat
      961 aaagtcgatc atttctgcca gtccaatttc tgtgtttgtt ttaaaatttg ctacttgtcc
     1021 tcgcgcatgg gtgccagcat ctgttcttcc tgatccaata atttctatct cttcatttgt
     1081 catctgatgc aatacttttt cgatttttcc ttgaatggtt ttatcgtcat ctccaagtct
     1141 ttgccatcct gaatatcttt ttccgtcata ttcgattgtc aacttgatgt ttctcatatt
     1201 ttagtggctc ctctgtcttc catttggtct tctctctacc tttttaagat tgtagcacaa
     1261 aatatagctc cttgcttctc taagacagaa gctatacaag aaaactgtgg aaatatatat
     1321 ttcagaaaaa caaaaaacag ccaccggatg aagtatccgc tgactgttca atttgacggc
     1381 tcaagtttta ctgtttgaga tgcaagatac tcaacctggc aatgggcaca taattaaaga
     1441 aaaaactgat tctttggcaa aaacctattt tattcagtaa tggccaagaa tttatagaag
     1501 gaattcttgc cagtccataa atcgccgcaa aaagcaaact gatcacagac agtccaaggt
     1561 ataagtggct tacgtccatt tgcccatttt ttcgatagat caagaaaaga agaactggaa
     1621 acaataaaaa accactgtaa cctaaacctg atccgatatt atggacccat gtcgagacat
     1681 tccacgaaga ttcatgggta ttgatactga acaaacctgt aaagatacaa tcacctatac
     1741 catagaacgc gatggctgct gtaactaggg tagctaacac acgagatgta ctaacaaatg
     1801 cagcatacaa tgcaggcaat gacaggatga aaaagatccc tgaaaccaca gaccaaataa
     1861 gaaacgcttt tcttacagga ctggaaacct ctccaaatac actgatcacg ctcttcattt
     1921 gatctaattc aggataatac aatcctaata cgtacggagt caaaaaatca ctcagcacgc
     1981 ctaataataa aaagaaaaaa ccaaatttac gtaaaaaatc catgatacgc ctccaataac
     2041 aattacctat gcttttaatt gttattataa acgactttat cttgatttaa cagaaacctc
     2101 tatttacacg aacatctata taactcatta aaaactcgaa tcattttaaa aatactaaat
     2161 atctaacgtt caacgttcac ttaatccatc taaacaatct ttcaatttga ttttttcccc
     2221 acttctttta cgcgcgccta gctctcctat tgcagtaaac aacagatcac ttgacgaatt
     2281 taaggcagtt tccattgaat cctggataac accgataata aatccaatac cgacgatttg
     2341 catcgcgaca tcattagaga taccaaacaa actacatgct aatggaatca ataacagtga
     2401 accgcccgca acacctgaag ctccacaagc agctaaagca gacaaaatac ataaaaggac
     2461 tttcaaaaca aaaggtgctt caatccctaa agaatgtaca gcagtcaacg tcatcaagga
     2521 aatggtaatc gcggcaccgc ccatattgat cgtagcacct aacgggatcg atatggaata
     2581 agactctttt gtcaatttca atctttcagc caacatcatg tttatcggga tatttgcggc
     2641 agaacttcgg gtaaagaaag cgggaagtgc gctttctttc aaacaaaaga aaatcaatgg
     2701 atagggattt tctttaataa acaaaaacgt gaggaatggg tacacaacta atgcgacaaa
     2761 cagcatcgta ccgatcaaaa caagtaaaag ctgcagatac tctgccaatc cagcgattcc
     2821 agtcgtagca attgattgat aaaccaatcc taaaatccct atcggagcaa atgcaataat
     2881 cacttgtacg acttgtgaca acgctataga tatctgatcc accaactctt ttgtcgattc
     2941 tgaacgtgct cgaaaagcta gtcccaacaa tacagcccag aataagacgc caaggtaatt
     3001 cgcatcaata agagattgaa caggattttg tgtgacattc gtcaacatag tagataaaac
     3061 actttccaaa ctttgcggtg ctttttcaga tactgtttct tgtaaagtaa taggaacagt
     3121 aaaaattgaa cttacaataa ctgctacaag tgcagaaagc aatgttccca ctaaatataa
     3181 gatcaaaata ggcttcacga atgtcttatt cccaatttta tgcttggcaa ttgacgccat
     3241 cgtcaagaaa aaaaccagaa taggtgcgat tgccttcaag caactgacaa acaacgttcc
     3301 caatattgaa ataaatgtcc atgtggggaa aaagatacca aacaatattc cgactaaaat
     3361 accaacaaca atacgttgaa ctaacgacat atccaaaaac ttctctaaca aatgaccacc
     3421 ctttcacaat tttaacaatc attgtatcat tttatgcaaa gaaactaaag gtataatttt
     3481 ctaaaaattc taacaataca aacaaccatt tcttgctaat tttgtacttt ttctgtctac
     3541 acgttgtttt ttgagatatt ctaagttagg atgttattaa tgggcaactt atcaggaaac
     3601 gttttcttat gtttttgtcc aatttttttg tgaggtgttt attcttgaaa aaaataaaga
     3661 aggagagtta tggcttcctg acaatctttg tcgcttgtat aatgatggtt ttacagcgta
     3721 tattgatttc agcagtcact attcctacat atgcaacaaa ccctggtcga ttctacatct
     3781 atacattttt acaaacttcc gttgttattg gtgcaaattt gatcccactt tacatgggtt
     3841 accaatatca aaaaatcgcc cgtttaaaag ttttacatta tttaagtcgt tttgccttta
     3901 tattcctaat agccacacta gtttgcaata tcttttttta cgtacaagct ggcagcttga
     3961 atattcgtga ttattggctt atcctaacac ctatcagtca gaattacttt acttatgctg
     4021 tttcttgtac tttgcttttt tgtagtattc cttatacagt tggattttta aataaattat
     4081 cgaaaacaac aattaaatat ctactattat ttttaacaat tggattagtt ggctgctcta
     4141 ctctatttaa taaagacccg tttgcattac agaatggaca gagtatttta tggatttttt
     4201 atttattttt aatgggttat ggtttgaaag aatggaactg gaaagaaaag gtccgctata
     4261 agcttcctca agtatttatc agcttga
//
LOCUS       DARDHA010000192         4081 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000192 DARDHA010000000
VERSION     DARDHA010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.196"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            47..1348
                     /locus_tag="SDA10_002598"
     CDS             47..1348
                     /locus_tag="SDA10_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4241468.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1375..1635
                     /locus_tag="SDA10_002599"
     CDS             1375..1635
                     /locus_tag="SDA10_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEG4241469.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2634..3869)
                     /locus_tag="SDA10_002600"
     CDS             complement(2634..3869)
                     /locus_tag="SDA10_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEG4241470.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1160 a          662 c          855 g         1404 t
ORIGIN      
        1 gtcatgcaat gaatggttca tattttattg aaaaggagtg gttttagtgg aaaagctgat
       61 gaaatgggtc gaagagaaac ttgtcccacc aatggcaaaa attggtacac agcgtcattt
      121 attagcaatt cgtaatgggg ttgtttcaac attgtcattg attttgattg ggacattttt
      181 tatggtcttt atcaatttac cgtttcctgg atggaatgaa tttattgccc cttattcggc
      241 aacgattgtt ttacccttta ggatgacgat gggattgatg gctatttatg caacttttgt
      301 tatgggttcg gatctagcta aatcatatgg attagatagt gtgacaggtg gtatattatc
      361 tttaggaacg ttctttatgt tacaagttcc tgtaaacgtt ttaactcctg aagaggcacc
      421 tcttggctgg gtcttaccta tgagttcttt gggagcatct gggatgtttg cggggatctt
      481 atctatgatt tttgcagtgg aagtatatcg tttctttaaa aatcgaaata ttactatcaa
      541 aatgccagaa caagtgccac ctgctgttgc ccgtagtttt gaggcattga ttccaggtgc
      601 agtcatttta acaattactt ggctgattcg aagcgtcgcg ggctttgatg taaacgctgc
      661 cttattatcg ttattcgagc ctttaaccaa tattttaggg aataatttgc ttggtgtttt
      721 actaccaatg tttttgatcc atttgttgtg gtcatttggt atccatggca tgagtattgt
      781 aggggctgtt gttcgtccaa tgtggctgat catgttggat gagaatgcaa aggcattggc
      841 agatggtaca gctgccaccc aattacccta tatcgctcct gaacagtttt atcaatggac
      901 ggtaacaatt ggtggtgcag gagcaacgat cgtggtatct gttttgttta tattcttctg
      961 taaatcaaaa ttcttgaaag aggttggtcg tttttctatt attcctggta tcttcaatat
     1021 caatgagccc ataatttttg gagctcctat gatgttgaat ccttacatgt tcatcccgtt
     1081 caacttagtt cctttagttt tgactattat cagttatttt gcagtcaaat tagaaatggt
     1141 caatagattt actgttttgc caacatggac attaccagct ccaatcggcg gttatctttc
     1201 agcaggaaat gactggcgtg ttgttatctt gatcatgatc aacacattga tagcatttat
     1261 tatctattat ccatttgtaa aagcttatga taaaaagatg ctgtcagatg aacaaagtcg
     1321 tggagaaaac gaagtccaga ctgtctaagt ttggttttta gaaggtggga aacaatgtat
     1381 tggctgattt tattttttgt ttttatcttt ttattaacgg ctagtcacct tatattgaat
     1441 atgctagcta catatcatat tcaaatcaat cgatggattt gggcactggc atcatttttg
     1501 atcgtgattc ttccaaagat cattgtaccg catatgaatg ttttgttttc atgggggaca
     1561 tatgtacttt gtggaatttt tgctatcaat tttatgatcg agcagcatag atggtttgtc
     1621 acatcgaagc tttaaatgtt ctaaaagtgg ttgtaacaga agtggttagc ttcaagaaat
     1681 aagaagaaat ttacgaaaat tgcttcttaa atttttgtga aatgttggct tatttttcgt
     1741 ttggttgaac ttattaccgc agatgcaagc ttttgaccac cgtttattcg gaatatggga
     1801 ctgtgacaag gcttttgtca cagccctttt ttacataaag attattattg ttttaactgt
     1861 tttggcagaa agaaggagga aaaaatggaa attttatcag caatggattt agaattagta
     1921 gatctactac caaagtgatc aagtcaaata tagatttggg agcttacaac catatagtag
     1981 gggctgcagt atggtgtatg aatggtaata tgtatgatgt gagtgtttat tcaaatcatg
     2041 aaacgtgtat agaaataatt gtgctgggaa tagcaatcag tcatggtgaa cgtgactttt
     2101 agtgtatcgt tgctgacgac atagaacatt ctgacatgat ctactcaata tgtggggatt
     2161 gctggcagtt tatataggag tatgcatcgg attgcgagat cattgtctca acagaagagg
     2221 gaggttgatt atcaaagata aagtggtaaa tcctacattt gatttagtaa ttgttgaggg
     2281 gactagcggc acaaatctct acaaagcgat gcttctagat ctatatgaca cattttaaga
     2341 aaatgtattt gcctaattat tatggtcttt cttttgaaga aacgttgaga tgatccaacc
     2401 agagaaaggt gtcaaagaat ttgactaaga ataaatggca gacgctacct atgctcattt
     2461 ggatagcgtc agatctttta ttttaaggat gattttgtgt tttttagaca ccgtaattta
     2521 agtaactcta acttttcaat ccactattta ctttttttgt attttcactg tctgggtctc
     2581 gaatagcgaa attactgatg agtctctatt aaaaaagctt tttctacttg aaactatatt
     2641 gggtggttgc tcaggaaggt ttcgatttct tgaattgctt gttgtggacc atttattgga
     2701 aaaacggctt gcagcgtgcg tgcctgctga cgaatcttgg gtgaagaaac ttcgcgcaga
     2761 gcaatctgca gttttctttc agtcactggt tttgtgaggg cgattgcgtt tccgtatttt
     2821 acacaatagt caatattaca gctttgctca aattgcatcc ccacaccgat aaacggtttg
     2881 ccggattcac aggcagtttg gacggtccct tccccgccgt ggataaaaga aaaatcaatg
     2941 aattcatgca aaagatgact agacagatat tctgttatta aaagatcttc tgagtcagat
     3001 tgaatgtcat caagcagata gtcaataggt gcaataaccg ctagatccgg ttgtttttgt
     3061 aagaagaaca aaattttgcg taacatacgc ggattgcctg aactacccat tgcgaaatag
     3121 ataatacgtt ttctttcttg ttgctttcgc tgtataaaag ttagcacttc ttcaggcaag
     3181 gaagtactga gcttagcaaa aattggacca acgattcgat aattttccgg cagctgttct
     3241 tcttccaaaa atagtttagg cgttgtgatg agatttagat cagcatccat catttgtaaa
     3301 cttgtttttg gtaaaggaag catttctttt tgtgcagcgt gtttgaaggc attggggatc
     3361 catcgggtat ttactatacg cttttttgtc cagtgccaaa ggggattgct taaagggccg
     3421 aagaaacgta actcgtgggg catcagcgca tgctctgatt gaaaaaaagg ttcactcaaa
     3481 gcatagggtt taacatatac taaaggaatc tttttattgc gggccgataa aaaaattgtc
     3541 acattcgaac ctgtcacaat cagtcgaggc tcaaacttag caatcagact ctgttcacta
     3601 gtgatacgag aggcaaccat ttctttggta aaagggtttt taagtgtacg caactggtca
     3661 aattttatga tttgttcttc ttgttttttc gtaaggatcg gcgctagtaa atgatactca
     3721 aagccacttt ctgtgatcaa aggagcaaaa gttttagaaa agccaaagaa tacacagtaa
     3781 taacggtctt tcatagcttt ggcaacctca atcattcgtg ttgtctcagc taaattgaat
     3841 actgcaggag caaaaagaat tgtcttcatg aaatcctcct tattggaatg agtctatccc
     3901 ttcgtttctt actagtatac taaaaaacac agcgttcgag tagcctatgt atattttgaa
     3961 tattaaactg tctctcgctg ttttcacaac ttctaaattt aaattttgta atcaagttga
     4021 aactccaatt ttcaaaaaaa cacgcaacaa ttttttgatc gatttatatg ttgtgccaga
     4081 t
//
LOCUS       DARDHA010000193         4016 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000193 DARDHA010000000
VERSION     DARDHA010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.197"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(232..381)
                     /locus_tag="SDA10_002601"
     CDS             complement(232..381)
                     /locus_tag="SDA10_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241471.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            843..1055
                     /locus_tag="SDA10_002602"
     CDS             843..1055
                     /locus_tag="SDA10_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241472.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1333..3132)
                     /locus_tag="SDA10_002603"
     CDS             complement(1333..3132)
                     /locus_tag="SDA10_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEG4241473.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3256..3852)
                     /locus_tag="SDA10_002604"
     CDS             complement(3256..3852)
                     /locus_tag="SDA10_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG4241474.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1443 a          711 c          688 g         1174 t
ORIGIN      
        1 ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt cataaaaaac
       61 gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag agagacaatt
      121 tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt acaaacgtct
      181 tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc cttatctaga
      241 ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt tattttcgtt
      301 ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc ggtattgcat
      361 atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta gttcttgaaa
      421 gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt tgatgcaatt
      481 aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct attttctttt
      541 aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat ataaatcata
      601 tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga acaacatagg
      661 aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata gttatctaaa
      721 aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt tcaaatacaa
      781 ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa agagggaatg
      841 aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga gtatctcatg
      901 aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat gttgaaagtt
      961 atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc gtaaaattct
     1021 gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga attcgcaaga
     1081 actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa aaagaaaaag
     1141 ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta ttttagctat
     1201 tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg tcaaacaaaa
     1261 aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac ttttttcatt
     1321 aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg aataagtgat
     1381 ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg ttatgatacc
     1441 atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg tccatacata
     1501 tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg tactatattc
     1561 cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag gtaagatgta
     1621 gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt acgtcaaact
     1681 ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa atcgttcgac
     1741 aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt ggtttagctt
     1801 atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc cgttttcagt
     1861 aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat ttacccctgt
     1921 taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg tttcctgaac
     1981 atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg cttctaaagt
     2041 attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg gttttgtctg
     2101 aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga aaaaaactgc
     2161 aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg cttgtgggat
     2221 aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa caatgatata
     2281 aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta aaagcgtcat
     2341 agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca tactttgtgc
     2401 caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc caatcacaat
     2461 aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt cccgaatggc
     2521 tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca gtcgaatccc
     2581 aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc aacgattagc
     2641 aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat cagaattaaa
     2701 acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca aaaaagtaat
     2761 gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca aagagattgg
     2821 ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga aataaactaa
     2881 cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat gttgagaaaa
     2941 aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta aaataaaacg
     3001 ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca tcagcaagac
     3061 atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta agctacgttt
     3121 caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat gaaaaaggaa
     3181 agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc aatgactaat
     3241 tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat aaatcaaggc
     3301 catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg tatatcgttt
     3361 cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag caactggaag
     3421 aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc agccaatata
     3481 tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat aacgacgccc
     3541 tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga taaatccagc
     3601 ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa tgattggaat
     3661 aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga tggaatcacc
     3721 tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa aataaatcgt
     3781 cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt ttcgagaggc
     3841 agcaatattc attattcatc aactccttag aggttctttg ttaactatac tttactagaa
     3901 aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg taagttcatc
     3961 ataagtataa aagattgatc tcaaaaaaac atccaactaa agacttattt aataac
//
LOCUS       DARDHA010000194         3913 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000194 DARDHA010000000
VERSION     DARDHA010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.198"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..260
                     /locus_tag="SDA10_002605"
     CDS             <1..260
                     /locus_tag="SDA10_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HEG4241475.1"
                     /translation="DSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKIQTIINEMS
                     DEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            263..1159
                     /locus_tag="SDA10_002606"
     CDS             263..1159
                     /locus_tag="SDA10_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG4241476.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            1209..1598
                     /locus_tag="SDA10_002607"
     CDS             1209..1598
                     /locus_tag="SDA10_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG4241477.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            1585..>3913
                     /locus_tag="SDA10_002608"
     CDS             1585..>3913
                     /locus_tag="SDA10_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG4241478.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         1312 a          625 c          824 g         1152 t
ORIGIN      
        1 ctgatagtgt tgacaaaatt gactttggca atggtataaa agttacagac gaaattgatg
       61 aacaccgcgg agaattgatt aagaatttag tgaccaatcg ggctaagatt caaacaataa
      121 ttaatgaaat gtcagatgaa attgatagtt atagagcaga gctgaataaa tcagattgga
      181 aagttaacta taatgaggtt tcaaagaggt atcaaagagg tatcaaaaac ttagtgaaga
      241 gttatctcaa gatgaattaa aaatgttaca aaaccttgat tctttatatc aatctttatc
      301 aaaaactgaa gagcgattgg aagaaattat tcaacaagaa ataaagctta aagattacca
      361 agttgaggtt gactcagcaa tgtcaagaat gaataaatca tatcaaaatc tctattctga
      421 gagaagtagt tcctcgcgcg aaattttttc tgatattaca ggaatagagg ctactattca
      481 accaaatagg gatttcaata attatgttaa agagctcaga gcttgtatta ataaggagga
      541 cacttttgac agtgagtttg gatctcttat tgataagctg acaaaaaatt ccattactaa
      601 tcaagatata tataatgaca tacttgatat aagagttagt ggggaaagta gtattttttc
      661 agataaaaaa ttaatcaatt ctataagaaa attgaatgat gcacaagttc ttgaattaca
      721 aacccttaag ccaaatgatt tagtagttat ccgacttgaa ctaaatggaa aaaaagttag
      781 tttatcgaat gctagtgctg gtcaaaagac atctgcaatt ctttctatga ttttggcgta
      841 tggaaattca ccactcatat tggatcagcc tgaagatgat ttggatagtc aattaattaa
      901 taacttaatc gtaagaagta tcattacaaa aaaggaaaac agacaaattt taattgtaac
      961 tcataatgca aatatcccag ttaatggtga ttcagaatgg ttgattagca tgggagattc
     1021 aaaagaaata tcggctgatc agtgcgggtc ggtagatgaa cccaatatca agccaagaat
     1081 atgtactgtg atggaaggtg gcgaggatgc ttttacaaat agagcgaaac gctatggttt
     1141 taaacaaatt gtagaataac actagagggt gagacatatt attatgtccg ccctttttag
     1201 gaggaaaaat gaaaaaaata aaaagctata ccagtatctg ggcagttgaa aaagtcattt
     1261 acgcaatcaa tgattttcag ttgccttttc ctgtgacttt taaccaaatg gcttggttcg
     1321 ttttatcttt actgtttgtc atcgtatttg cccatgtgcc tccactatct atgatagagg
     1381 aggtcttttt aaaatactta ggcataccgg tggctgtcac ttggtttatg tcgcaaaaga
     1441 cgtttgatgg caagaaacca ctgggatttt tacgatcctt tatcagctat cacttgcgat
     1501 ttaaggtgac ttttgcagga aagaaagtca aagaacagaa gaaacgatgg gatgaaccca
     1561 gtactcttgt aaggagtgtg aactatgtat ccgattaagt atattgaaaa taatctcgtc
     1621 tttaaccaag aaggcgagtg ttttgcctac tatgaattag tgccttacaa ttactccttt
     1681 ttgtcacccg aacagaaatt tcaagtgcat gataactttc gccaactaat tgcgcaaaat
     1741 cgtgaaggca agatccatgc gttacaaatt gccacagaaa gtagtattcg cgccacacaa
     1801 gaacgatcaa aaaaagaaat taccggtaga ctcaaagaag tagcgaaaca acgaattgat
     1861 atacagacag atgcattagt atccatgatt ggcgatagtc agattgatta tcgctttttt
     1921 attggcttta aactaattgc gacagatgaa gaagtcaatc taaagagtct caagaaatct
     1981 ttcttttctg ggcttcaaga atttgtctat ggagtgaatc atcatttgat gggcgatttt
     2041 gtttccttat ccaatgaaga aattcgctgc tattctaagc ttgaaaaatt aatggaaagc
     2101 aaattggctc gaagatttaa agtcaggcga gtcacaccga gtgacctgac gtatttgatt
     2161 gaacatattt atggcgaaaa agggataccg tttgaagaat acgaatttca gctaccaaag
     2221 aaaaagctaa aatcagaaac actggtgaaa cgttatgact tattacgtcc aaatcgttgt
     2281 ttaattgaag aaaaaccaag gtgcttacgg atggaacatg agaatcatga gtcgtacgta
     2341 gcgtatttaa caattaatac aattgtgggg gagatggaat ttccttcctc agaacttttc
     2401 tattaccagc aacaacaatt tacattccca atcgacacaa gtatgaatgt agagattgtg
     2461 acgaataaaa aggctttagc taccgttcgt aataagaaaa aagaactgaa agatttagac
     2521 aatcatgctt accaatctga caatgaaacg aattccaatg tcttagatgc tttggattcc
     2581 gtggatgaat tggaaaccac cttggatcag tcaaaagaat ctatgtacaa actgagttat
     2641 gtggttcgag tgagtgccga atcagtggat gaactaaagc gacgctgtga tgaagtgctc
     2701 gatttttatg atgataccaa tgtaaaacta gtccggccct ttggcgatat gatggggcta
     2761 catgaagaat tcttaccctc aagtaaacga tatatgaacg actatattca gtatgttacg
     2821 tctgattttt tggcggggct tggttttggg gctactcaga tgctaggaga attagaaggg
     2881 atttactttg gctacaatgt ggatacaggt cgtaatgttt acctgaaacc ggcattggct
     2941 tcacaaggag tcaagggttc agtaaccaat gcgctggccg ccgctttcct gggttctctt
     3001 ggtggaggta aatcctttag taacaatctc ttagtctact atgctgtttt gttcggtggt
     3061 caagctgtga ttgtcgatcc aaaaggggag cgaggagggt ggaaagaaac cttgccagaa
     3121 attgcggatg aaatcagtat cctcaacttg acgagtaagt cagaaaatca aggtctgctt
     3181 gatccttatg tgattatgaa gaaaaagaaa gactcggaaa gtttagcaat tgatattctg
     3241 acttttttaa caggaatttc aagtcgagac ggggagaagt ttcctgtgtt acgtaaagcc
     3301 attcgaacgg tgactcaaag tgatcaacgg gggttattac aagttattaa cgaattgcga
     3361 aaagacgggt caccaatcgc ggaaaatatc gccgatcata tcgagtcctt taccgattat
     3421 gattttgctc atttgctttt ctcagacggc tcgattaccc aatcgattag tttagataaa
     3481 cagctaaata tcattcaagt ggcggatttg gttttaccgg atgcagaaac gacttttgaa
     3541 gaatatacca ccatggaatt gcttagtgtt tcgatgctaa tcgtcatttc cacttttgcc
     3601 ttggacttta tccatagtaa ccgagaaatc ttcaaaattg tcgatttgga tgaggcttgg
     3661 agttttcttc aagtggctca aggaaaaacg ttatcgaata agcttgttcg tgctggtcgg
     3721 gccatgaatg ctggggtcta tttcgtcact caaaatagcg acgatttatt agatgaacgt
     3781 ttaaaaaata atatcgggtt gaagtttgct tttcgcagta ccgatattca tgaaatcaag
     3841 aaaaccttgg aattctttgg tttggatcag gaggatcaaa gcaatcaaaa acgcctgcga
     3901 gatttggaaa acg
//
LOCUS       DARDHA010000195         3876 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000195 DARDHA010000000
VERSION     DARDHA010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.199"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            158..2257
                     /locus_tag="SDA10_002609"
     CDS             158..2257
                     /locus_tag="SDA10_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEG4241479.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2453..3316
                     /locus_tag="SDA10_002610"
     CDS             2453..3316
                     /locus_tag="SDA10_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEG4241480.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3421..>3876
                     /locus_tag="SDA10_002611"
     CDS             3421..>3876
                     /locus_tag="SDA10_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG4241481.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
BASE COUNT         1544 a          646 c          782 g          904 t
ORIGIN      
        1 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
       61 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
      121 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
      181 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
      241 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
      301 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
      361 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
      421 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
      481 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
      541 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
      601 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
      661 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
      721 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
      781 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
      841 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
      901 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
      961 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
     1021 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
     1081 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
     1141 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
     1201 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
     1261 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
     1321 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
     1381 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
     1441 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
     1501 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
     1561 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
     1621 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
     1681 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
     1741 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
     1801 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
     1861 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
     1921 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
     1981 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
     2041 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
     2101 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
     2161 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
     2221 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
     2281 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
     2341 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
     2401 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
     2461 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
     2521 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
     2581 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
     2641 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
     2701 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
     2761 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
     2821 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
     2881 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
     2941 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
     3001 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
     3061 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
     3121 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
     3181 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
     3241 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
     3301 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
     3361 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
     3421 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
     3481 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
     3541 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
     3601 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
     3661 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
     3721 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
     3781 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
     3841 catggaatct gtcaggaatg ctatgaaaaa gaaaaa
//
LOCUS       DARDHA010000196         3875 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000196 DARDHA010000000
VERSION     DARDHA010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.200"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            174..518
                     /locus_tag="SDA10_002612"
     CDS             174..518
                     /locus_tag="SDA10_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEG4241482.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="SDA10_002613"
     CDS             519..938
                     /locus_tag="SDA10_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEG4241483.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="SDA10_002614"
     CDS             complement(1030..1380)
                     /locus_tag="SDA10_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241484.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2566)
                     /locus_tag="SDA10_002615"
     CDS             complement(1346..2566)
                     /locus_tag="SDA10_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HEG4241485.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2932..3498
                     /locus_tag="SDA10_002616"
     CDS             2932..3498
                     /locus_tag="SDA10_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4241486.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3643..3730
                     /locus_tag="SDA10_002617"
     tRNA            3643..3730
                     /locus_tag="SDA10_002617"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3675..3677,aa:Ser,seq:cga)
BASE COUNT         1423 a          664 c          753 g         1035 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caactatcat ttaggtcaaa actaaaaatg
     2761 taagggaacc cattgaattt aaccaaattt ttttaatttt gcaaaaaaaa gttaaagaag
     2821 actgaaaaaa aatttaaaat ataacgtttt gagtaaaatg tttgcaaaaa atgctaaaaa
     2881 tagggtacaa taaaacaaca tatactgcag taaagaagtg ggggagtttt aatgagaaga
     2941 aaagtctata ccaaggatca aattctaaaa gctgcttatg atgttgttgc aaaggaaggg
     3001 tttaaaggat ttactgcgag aaatattgct aaaaagatgg ggatttcgac acagccgatt
     3061 tatctggaat tcaaaaacat ggaagattta aaaaatacgt tgctggattc tatctttgaa
     3121 caactaaaag aagatgtttt ccctattgaa cgtactggtg ataaaatcat tgatctcggt
     3181 ttgaactata tctactttgc aaaaaagcat catagtcttt acatggcatt gtacattgat
     3241 gaacatgggg acggaaagaa aatgcatgat ttttcataca attatttccg tgaaatagtt
     3301 aaagaagatg aaagatacaa tcaattatcg gatgaatatg tcgatgctct gcatactggt
     3361 acatgggtag cagtgactgg cttagcgtca ttgatgtcat caaacatcat gcatccaaca
     3421 gaagaacaaa tcattgcact gatcaaacgg acaattgact cgattttagc attagataat
     3481 cctgaaataa tcagatagtg ttctatcagt atgaatttga tcggtaagtc cgttggctgc
     3541 aaactgtcaa tggacttttt tgctaaaaaa agcgatttct gttatttatc tattagaaat
     3601 aggcttgaca tttcgtgaaa aaagtttaaa aatagtactt acggaaggtt ggcagagtgg
     3661 taatgcaccg gactcgaaat ccggcgaacc ggctaatacc ggcgcgcagg ttcaaatcct
     3721 gtaccttcct tatatcaacg attagaggtt gtgaagaagc tgtcctgcat caagtaataa
     3781 gaacggccaa acaaaaatag cttttcctat tttttcgttt gattgggctt attaccgcag
     3841 atgcaagctt ttgaacaccg tttgttcgga ataag
//
LOCUS       DARDHA010000197         3862 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000197 DARDHA010000000
VERSION     DARDHA010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.201"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            10..900
                     /locus_tag="SDA10_002618"
     CDS             10..900
                     /locus_tag="SDA10_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEG4241487.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(973..1797)
                     /gene="nadE"
                     /locus_tag="SDA10_002619"
     CDS             complement(973..1797)
                     /gene="nadE"
                     /locus_tag="SDA10_002619"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEG4241488.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1809..3281)
                     /locus_tag="SDA10_002620"
     CDS             complement(1809..3281)
                     /locus_tag="SDA10_002620"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEG4241489.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT         1128 a          793 c          682 g         1259 t
ORIGIN      
        1 gtgatatata tgcaactaca aataaacgaa gaatccttac cggtatatga agcattagct
       61 agtaaaacaa ggatcaagat catccagcta ctttccaaaa aaaagatgaa tgtcaaagat
      121 ttggcaaaag aacttggagt gagcagtgct atcacgacga tgcacgtgaa aaagctagag
      181 gaagcaaaca tcatcaaaac agaaaaagta ggacaacaaa aaatttcgag ccttcgtgtc
      241 gataaaatcg atattagttt tccagaaaaa atattcaatg cttttgatac gaaagaaaca
      301 tcgataccta ttggtcacta tacaaattat gcaattgaac caacatgcgg tttagctacg
      361 atccatgatt ttatcgggaa agtggatgaa ccacgctatt ttatggatcc tcgaaggatg
      421 gatgcccgca ttctctggtt taccagcgga tttgtcgagt atcaggcacc taatttttta
      481 aatacagagg atacattgga gatgttggaa gtttctgtgg aaatttcttc tgaatttcct
      541 ttttcaaatg acaattggcc ttcagatatc actttcagcc taaatggtgt ggagctagga
      601 acatggacga gccctggaga ttttgctgat atccgcggta aatatacgcc tgattggtat
      661 ccagataatc ttaatcaata cggcttgctg aagacgatcc gaattaccaa acacttgacg
      721 aatatgaatg gagaaccctt gtcaaacatc acgatcaacg acatccctaa agagcaagac
      781 acttggcatt tgcgaatcga agtgaaagac gatgccaaac atgtgggggg ctgtacttta
      841 tttggtaaag gattcggtaa ttacgatcag gatatcaaat taaaagttta ctacagctga
      901 tatttttctt ttctatttta taaaacaaaa acagccaatc cctatgaagg gacggctgtt
      961 tttgctttat tgttatttcc agaaatcatc caaaacggaa attggcaaat gtcgtttatg
     1021 ttgtgtcttg ttccaccatg cttcgatttt ttcttgtgct tgagcgggaa cttcttttcc
     1081 ttctagataa tcatcaattt cattatatgt gacacctagt gcgacttcat ctgcaatcat
     1141 tggtttccca tcttcaagat ctgcagtcgg aatttttgtg tataattttt ccggtgcatt
     1201 caatgcttga agcaattgtt tcccttgtcg tttgtccaaa cggaataatg gcaaaatgtc
     1261 ggctccacca tcaccaaatt tcgtgaagaa tccagtaatg ttttctgcag catgatctgt
     1321 ccctaatacg gctcctgctc tttctccagc tacggcatat tgcgtaatca tacgttgacg
     1381 tgctttgata tttcctttgt taaaatcgct gattcccaca cctgcttcgc ttaatacttg
     1441 tacttgtgca tcaacagctg gtttgatatt tactcgcaga ctaacatcgg gctggataaa
     1501 gtcaagagca gcttttgcat cttcttcatc tgcttgttca ccataaggaa gacgaacagc
     1561 gataaattga tagttttcat cttttgtttc ttcacgcatt tcttccatcg ttagttgcgc
     1621 caaacggcct gctaaagtag aatcttggcc gccgctgata cctagtacaa aagttttaag
     1681 aaacggatat tttttcaaat atgctttcat aaagtcaata cttttacgta tttcttcttt
     1741 tggatcaatt gttggtttga cacctaattc attgataatt tctgtctgca ggctcattta
     1801 ttctcctcct atgcttctgt atgaagttct gctacgcttt ttttcattcg ttccatgata
     1861 gccattttat ggttccagca ttcagtagac agatcgactg ggtatttttg cggattcaag
     1921 tctcgtttat actcttccca tagtgaatct agattttctt ttgtatattc tttgatttcg
     1981 tttagtgcag gtaattcgta aactcttttt ccttcgataa agatatcttg taaaactggg
     2041 cgagctgtaa agtctcgaac agttttattg ataaacgtat gcactggatg gaacatgaag
     2101 atagcttctt cttctcgagg gtcttcatcc cataatgtca cgtagtctcc ttcagatttc
     2161 ccatcaaaat ttcgagtgat gcgccacact tgttttttcc ctggagtagt tactttttct
     2221 gcattgcttg ataacttgat cgtatccatc atttttcctt catcatcttc gatcgatact
     2281 agcttgaata ctgcaccaag ggctggctga tcataggctg tgatcaattt tgtcccaaca
     2341 ccccatacat caattttagc tttttgcatc tttaaattca aaatcgttgc ttcatccaag
     2401 tcattcgaag catagatttt tgcttccgta aatccggctg catctaattg ttcccgaaca
     2461 cgcttagata tataggccat atctccactg tcgatgcgta cgccttggaa gttgattttg
     2521 tcacctaatt ctttcgccac gcgaatcgca cttggcacac ctgatttcaa cgtatcataa
     2581 gtgtcgacta agaacacgca gtctttatgg gttttagcgt aggccataaa tgcttcataa
     2641 tcatttccat aagattggac taacgaatga gcatgggtcc cgctagctgg tatcccaaat
     2701 atcttcccag cacgtacatt gcttgttgca tctgctccgc cgatatatgc cgctctagtt
     2761 ccccagactg ctgcatccag ttcttgtgcc cgtctagttc caaattccaa tagtggatct
     2821 tctccgatga cagatttgat acgagctgct tttgtcgcga tcaacgtttg gaaattgacc
     2881 atattcaaca gtgctgtttc tactaattgg cagtgcgcta aaggcccttc cacttggatc
     2941 aatggttcat ttgcaaaaac tagttctcct tcacgagcag aacgaactgt tgctttaaat
     3001 tcaaaatttt gtaaatactc caaaaaacct tcaggataga cttctaatcc acgcaagtaa
     3061 gcaatatcgg aatcattgaa cgtcagattc tctaaataat tgactaaccg ttccaatcct
     3121 gcaaaaaccg cgtatccgtg gttaaaaggc atttctcgaa agtaacattc aaaaacagca
     3181 tgtaaatcag ctcgacctaa ttcccagtat gtctgcatca tattgatttg atatagatcc
     3241 gtatgcagcg tcaaactgtc gtctgggtaa gtattattca tcttgtatta ctcctcttgg
     3301 caagttttca tttcctaccc attataacaa aaaaaattaa taggaaaaga aatcttccat
     3361 tttttctgaa aaagcacatg ttttacggat atttcttgct aaaaatgaac tccatctgct
     3421 aaaactatcc tttcttattg gtatttaacc taaacgagaa agaatactta ttttattcaa
     3481 tgtatccgag acacgaagaa attagcgttt tctatttttc ttacttgata aaataagtag
     3541 attttcctac tacaatcgta gccgttaaga taccgatgat tgaacaaata taaccaaatg
     3601 tctctacaac ataatcaatg gcaaaagttt ttgaagcaat aatacgtctt tccttttttg
     3661 cttcttatcc ccaaagaaac ggatataact tattatgtat tgaatttttt tggaaaggcg
     3721 aattatgaca agatatacct tgcgttgaaa aaactcgcgg aaaaaatatc acacgttttt
     3781 aaaaagatac aaagtaagaa agcagctgtc aatagaatgg aaacaaaaat ggcgagacat
     3841 caatcagtct cgccttgttt tt
//
LOCUS       DARDHA010000198         3835 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000198 DARDHA010000000
VERSION     DARDHA010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.202"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(7..549)
                     /locus_tag="SDA10_002621"
     CDS             complement(7..549)
                     /locus_tag="SDA10_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEG4241490.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            684..1073
                     /locus_tag="SDA10_002622"
     CDS             684..1073
                     /locus_tag="SDA10_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241491.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1077..1841
                     /locus_tag="SDA10_002623"
     CDS             1077..1841
                     /locus_tag="SDA10_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG4241492.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            1970..2917
                     /locus_tag="SDA10_002624"
     CDS             1970..2917
                     /locus_tag="SDA10_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4241493.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3047..3787
                     /locus_tag="SDA10_002625"
     CDS             3047..3787
                     /locus_tag="SDA10_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241494.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1268 a          660 c          792 g         1115 t
ORIGIN      
        1 tgtgagttat tttccttgtc gttttctcac ttttttagtc cagaaaccac ctgaaatata
       61 ttttggctca taagaaatga taaaagcacg ctcttcgatt tctttgatca ggcgatacag
      121 ttcacgttcg ctttttctag gagaaagtat ttcgagaact agccgctcac cttccagccc
      181 cattgcagaa gtctgtgtca caccataacc gtgttctctc agtatacccg gcaaactttt
      241 atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg atgtatccta atgccaattt
      301 gtcttcaatc aaaattccga cactgatacc tactgcgtaa cctaacgcat agacaaataa
      361 attcagtgga ttatctaaac gatctaaaac aagggataag ccgacgatat aaatcgtgat
      421 ctcgaccata ctgacaagtg gagcaatcag tcgatagcct ttcattgtca acatgaaacg
      481 taacgtgttc agagtgatat aagcaaaatt aatgaggaag atcatcccca acatttttgg
      541 atcaatcatt tgtaaaactc ctttcattta tataacaaca atatcaaaaa aaatcgtaag
      601 tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata aaatgattat aatagaatta
      661 taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg tgtgttttgt gccagccacg
      721 tgctaagtct ccttctcagt atatacttag gtgtctattc gattcaacag ccaattgagg
      781 tagagaacat tgtttttcag atgtcgctga cagatggttt aattttaagc gtttttttct
      841 ttttgatact gtttattggt aatattttcg gtagcgttgc cagttttttg aatttttcta
      901 tttatccact cctttcgcta gcattaggag tggtgggact ttgttctctg tgcttatttc
      961 ccgagccatt cagtcctgca tttatattat ttggtacact gaacctgttc cagacaactg
     1021 ttggagcatg gttgctatgg cgatcaggaa atttaatgaa aataggtgag taacaattga
     1081 atccaattat attgaaacgt tctgtccatc atattccagt gttagaagtt gttctgaaag
     1141 aattaagaga ggaacgtatt cccgttgtca tctattatca cggatggcag acaagtaagg
     1201 agttagtttt gacgcaagga agaaaaattg cgcagcaggg cattcgtgtg atcctgccag
     1261 atgcgatgaa tcatggggag cgcaaacagc cggtttcttc tattccatcc tatacatttt
     1321 gggatagtat ctacggaaat ctgtttgaat ttgacacact tatcaagcat ttagaaaaaa
     1381 gaggtcttct acatgatcag cttgcagttg gcggggtttc gatgggggga atgacaacat
     1441 gcgccttgtt ggctaaacat cctcatatag tagcgggcgt gtgtttgatg ggttcccctg
     1501 cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc ggaatataat tatgtcgtac
     1561 caaaagacta ttttgatttg atttcatggg ttaagagcta tgatttgtca ttgcagccgg
     1621 aaaagttagc tggacgtcct ctgttctttt ggcatggaca agctgacgaa aaaatccctt
     1681 atatacaaac ggaacgattt gtagaaaaaa atcggcataa gtcttacgga aaagaaattg
     1741 tttttcgttc ctcaaaaaca cagcgacata tggtacaaat accactaatg gaagaagctt
     1801 ccactttttt agcaaaaaat ttgataaaaa agtataaata attgaaacgg aaaatcactc
     1861 atgacgatag aggattttcc gttttttatt ttgggctata ataaactctg ttaggaaaat
     1921 ttggtataat tgatagtaag aatgacaaca tgttagaaag gaagagagca tgcccacttt
     1981 agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg gtctctcgag tcatcaatca
     2041 tccggaacag gtgactgacg aattgaaaga gttagttttc tcagcaatgg ctgaactgga
     2101 ttaccgtcca aatattgctg caaaagcact tgtcacgaat cggtcgcaga tcatcaaatt
     2161 atttatactg gaagaaatcg acacaactga gccctattac atgaatttgc tgatgggaat
     2221 cgctaaacgg atcggtaaag cgcattattc tctccaatta gttacaaatg atgccttcga
     2281 cgtcggttct tgtgatggat acatcatcac aggtgtcaga aaaaaagact tcgattggat
     2341 cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat tctgaaggat atgattatgt
     2401 agacagcgat aatgaatatg gtacagcaaa agcaacgcaa tatgccttag aaagagatta
     2461 tcaaacgatt atttatatcg gtatcgaagt ggacgagcct tttgaaattt caagggaaac
     2521 tggttattta tctgtgatga aggaagttga tcttcctagt aaaatcttgc ggtttccaaa
     2581 tcattcgaca gaagtagaaa gatatatgac agaaaactgg gcagatttta cagataaaac
     2641 ttgttttgta tgtagttcag atcggctagc actaggagca gtaagaggga ttataaatgc
     2701 tggaggacac ttgcccgaag aatttggtgt gattggattt gatggggtat ttttagacca
     2761 aatcagttca cctaaattga caacgatcaa gcaagatatt atacgtatgg gtgaagtatg
     2821 tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg agtcaaggtt atcgtcgttt
     2881 ttttcctgaa ctagttatca gagagacaac gaattaagtc ttattactat atatatattt
     2941 atgaaattca tcgctatttt cttaaaataa tcagcgcatt gctactgaaa acaatgaatc
     3001 tgtgctatta ttgagaaaag ttccattttt gagaaggatg gtttgaatga aagaaatcag
     3061 acgaaaacat ttccgttttc ctgcgtacga tgatgagtta ggcgtaaaat taaccaacga
     3121 caagaagcga gtattatttg acggacaaga agatcttttg actgaacaga atcagtcccc
     3181 gtttgaacag atgaaagaat ttcatttcac gaacacgaac accgagaaaa tccgtcgcaa
     3241 gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag gaaaatttgg aaaagcataa
     3301 aggaaaatta ccggattata ccggccatta ccaatcagaa atcacatcga caggacgaaa
     3361 aaaacttttt ggtgattcct ctcgttctgt cagacaaagt gaaacaagga cagagtccaa
     3421 gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg gagcgttctt attttgttcc
     3481 taaatatgta cccgcatcga ttatcccaga ccctagagaa agcaaaattt ctgaggatca
     3541 tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg ttgttcgata cagaaccgac
     3601 gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga acattcaatc gaacaaaaac
     3661 gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa aaaagccatg gtatcttaga
     3721 acgctcatta caaggattga tcgatgatca aggaaactct ttgggagaaa atagctattt
     3781 taaataagtg atatacaaat ttatattcgg ctacagttgg aaaggaaacg ctgcg
//
LOCUS       DARDHA010000199         3762 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000199 DARDHA010000000
VERSION     DARDHA010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.203"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            513..1553
                     /locus_tag="SDA10_002626"
     CDS             513..1553
                     /locus_tag="SDA10_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEG4241495.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2377..2709
                     /locus_tag="SDA10_002627"
     CDS             2377..2709
                     /locus_tag="SDA10_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEG4241496.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3328..3669
                     /locus_tag="SDA10_002628"
     CDS             3328..3669
                     /locus_tag="SDA10_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HEG4241497.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1388 a          489 c          690 g         1195 t
ORIGIN      
        1 gattaacaca gtagtatctt attaaataag ttaattttaa tggtagtaca taattattat
       61 atatatctga cattggtagt acttattgta ttttttagta gtatataatc tattgttttc
      121 tagcctaaag ccttgtctta taagaataat ataatatata ctactgaaat aaggtagtat
      181 atattactag taatggtagt atgttttata tgcataaggt agttcttaat ttattttttg
      241 ttttgacact ggtagtactt aattatcttt aattttaaaa ctaaatttta ttcatacgtt
      301 tttctggtag tatttaatta taaataatat ttatttaaag tagttcttaa tgactttaaa
      361 ggtagtacat aattcatttt atttctgatt ttaggtagta tttaattaaa aaacagtttt
      421 aatgcgtttg cattttttat ttatctttgc tattttcgaa aagagtaggt aattttacat
      481 aaataaaaca acgtgaaagt agaggatcta caatgactga ttttaaattt tttaaggcgg
      541 atagagttta caacgaatta ttttatcaat ttccaaaagt ctttattgtt tctgacgaat
      601 acaaaaaaat gaaagattca actaagattg cctatatgct tttaaaagca agattagaga
      661 tcgcaatcag caaacggcaa atcgatgaag aaggtaatgt ttattttact tatacgacaa
      721 atgaactatg tagagtatta aactgccaaa aacaaaaagc gatagcaatc aaaaaagagt
      781 tggaatcctt tggtttatta ttacaaaagc agatgggatt taacaaacag ttagggaaaa
      841 ataatcctaa tagactatat ctagcagaat taaaagtctc agaaaatgat atctacttac
      901 tcgaaaaatt tgatagagag aatagggaaa acgttgataa atcagagggt atgaaaatca
      961 tacccaccct cgacgaaaaa tcagacgctg aatcccttgg ggctcaagag ggtatgaaaa
     1021 tcataccgtg ccaaaacgtt gataaatcag agggtatgaa aatcatacca gaacttaata
     1081 ataatatatt agacactaat agacacaata tagacactga aaaagaccgc ctacaagatc
     1141 aattgttgtt agacaatttt gagacaatta tgacaaacga cagcattgct acgtttgtcc
     1201 ctgaacgagt attaaatttg ataaaaacat tttcttcaag ttacagtgaa gctcaaaaaa
     1261 ccgtccagac tattcataat gcaaagaaaa aagctgaaat agaaagtggt atttcgatag
     1321 tttttgaaga actcgatagt tattatgtca atgcagaaca agaattatac acgacactgt
     1381 taaaagccta tcaaaaatta aaaaccgaaa aagtcgaaaa tatccagaac ctgatttttg
     1441 tctatgtaaa aaattggttt atcgaaaaac caatagctgc taaagtatca agtgaaaaac
     1501 gtttgaatta tgaaagctcc ccaagcacta ttacgaaaga ctggttagag tgaaacttta
     1561 ctgacaaagt gattgttgct tcatggagta acattaaaat taaaaatttt ttattattct
     1621 aattatatta cgaatattac aaaataatag ctattttaat agaatttata agttaaaata
     1681 ttatgataaa aatcacaaac gtagcctttt acgtcataag tgttaaaaga aataattaat
     1741 ttcaaatata gaaaagggaa cgtatgtttg catactcctt ttttttgagt tataataata
     1801 cgcgaagaga gataatttca ttatttaaac aagctaaaaa tcttgatatt acttcttttc
     1861 aggcaatacg aatattattc gcttacattt tttgtataaa tttaattgtc tgctatgctt
     1921 gtgatgaatt gattttcgtt ctgcactttt ggatgataaa tttggacaat tgaaaataca
     1981 aaaaaaagaa aggcggtatg aactcggaag gctggtaacc ttcgtcagcg ttcatacacg
     2041 gcattaagat ttggccctta atgcgttaac ctttcagtgt tacggtatta cccgcaatgg
     2101 atttagtata acagatattc aaaagtgata ccactttttt tggctgtgtt aaggttaaaa
     2161 atacgtgata ttacgatgaa tctatgccta agcacaatgt tataaaaatg tccgtgtggc
     2221 tctttgccgt atgacgaacg ctgaaattag agggaacgca tgtttggatg cgttccctct
     2281 tttttgtttg tcatgaacga aaatcaattc tctatttttg aaaaataaca agaaaaactt
     2341 gttaaaaata tttaaagaaa ttgggtggtt tattttatgt catatacagg aattttgagt
     2401 ctcaaagata tttgtcacta cggtaaacgg tgtaccgcta cagagaaaat cacgaagaaa
     2461 ctatcaactg gccaaagtaa aaccgttgtg cagtgtaaga aatatataat ccagaaagac
     2521 aaagtctcag aggaaatgat ttattacatt gggaaacaaa agcaaataat tttaaaagat
     2581 cctatcccct taaaagagct atatccaaca atcaaacatg tttatgacca aaacggtgta
     2641 ctgattggtc gaagaaaaaa tggagtcttg cgttgtaccg caaaagggat ggggcgtttg
     2701 atcggctgaa ttgatcattc tcataacttg tattttatcg tttttcgctt gcttgaaaat
     2761 attgtcgaga cttctaaagg aacttagagt cactagaaaa ataaggagta gacttgatct
     2821 atcaacaatt cccttcgggg agtgacgggg taggttttaa cgattggtaa cgtaaaaaat
     2881 tggtcaatcg agtaaatgcg cttgtttgga tagtggtgaa atgaatttgt gtaaataata
     2941 agcgcagagt aaacaagagg gaatttagaa gttagacaag ttagaatagc gtctagatta
     3001 atcatgagca gctactaagg gatctagact ttgacgcaaa ctcatggaat aaggagtagg
     3061 tcttctcgta atggggagga cgcacaagaa attgtgtcgt gtgctgcagt acatgtagat
     3121 acttgcgagg acgttgatta ttggatcaat cgagcattta ccgctgtcta ctagggtttc
     3181 tcgtttcgga cgccataaac gagtcgaaat tttttatgta tttttaagta aacgaaaaac
     3241 gataaaatat gaacaagtcc tgaataaagt gaaaaaattg atataaaaca tagagaaaat
     3301 gaaatttacg taatggagga atgacaaatg aaaaattatg atccgaatat acgattgggc
     3361 acacatacta ttaaggtttc tttccaacgt tgggattaca aaggatttgt gacgtttaga
     3421 agaggcggaa attgtaaagg attagatgtt ttagcattag acgaggatga tttatacgat
     3481 caaacattga ctgataatcc aattggattc ggcttgctac ctgaagatga tgaaggtaat
     3541 gaatggttta aaatgacgct tatgaatgat aatggagacg aattatctgt tgaagatact
     3601 tggtcatatt tgagtgatta tattgtcagc tttgaaatca ttgaatttgt agcggataaa
     3661 gaagaataaa aactggagga ataaatttta cagataaagg cttagaagga gtcacgatga
     3721 ttaataaaaa ggtagtatta ccttaggcat tgagtaagga at
//
LOCUS       DARDHA010000200         3644 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000200 DARDHA010000000
VERSION     DARDHA010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.204"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            344..1063
                     /locus_tag="SDA10_002629"
     CDS             344..1063
                     /locus_tag="SDA10_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG4241498.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            1065..1907
                     /locus_tag="SDA10_002630"
     CDS             1065..1907
                     /locus_tag="SDA10_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG4241499.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            1904..2854
                     /locus_tag="SDA10_002631"
     CDS             1904..2854
                     /locus_tag="SDA10_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEG4241500.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            2869..3102
                     /locus_tag="SDA10_002632"
     CDS             2869..3102
                     /locus_tag="SDA10_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HEG4241501.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            3433..>3644
                     /locus_tag="SDA10_002633"
     CDS             3433..>3644
                     /locus_tag="SDA10_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4241502.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLE"
BASE COUNT         1310 a          447 c          670 g         1217 t
ORIGIN      
        1 atggagttta ctgattttca tatttgtata tggaacagac atagtcttga ataattttta
       61 taaatgtttt gatgcttatc aacttattca acccgctatc cacttcaagt ataatttttc
      121 catacgccaa taaagtagtt ttaggagcat gttctcattt gagaacgctc cttttttata
      181 tattttgaaa gctacttact atacgcgtag tttcaaatag ttttgtcatt ctaatcttca
      241 gcaacagaaa aaaatctaaa ctataaaatt tagggttgac taaaatataa tttttgtata
      301 ccatatgatc gtgataatca ttatcattta gaaggagaaa gttatgttag aagtgaaaaa
      361 gcttaatgtt tgttataacg attttttagc attgaacgag atttctttaa aaatacaaga
      421 aggtgcttta acaggtatta ttggtccaaa tggtgctgga aaatccacat tgcttaaagc
      481 aatgttaaat attatccctc atcagggaga ggtctccatt aataatgaag atatcaataa
      541 aaaactggtg aagattgcat atgtagagca aaaagctgat attgatttta cttttccaat
      601 aaaagtaaaa gaatgtgtat ctatgggaac atatgccgga atgaaagttt ttcaaagaat
      661 taaaaatgca gaatggcaaa gagtttcaaa ggctttggaa aaggtcgata tgggaaaata
      721 ttctaatcat caaataggag aattatctgg aggacaattt caacgtgtac ttttagcaag
      781 atgtcttgct caaaatgcgg atttcatatt tttagatgaa ccatttgtag ggatagactt
      841 agttagtgaa cgaataatta tggatacgtt aaaaaaattg aaacatcagg gaaaaacagt
      901 gctaattgtg catcatgatt taagcaaggt aaagaagtat tttgataata ttattctttt
      961 gaatcgaaat ctaattgctc acggaagtgt agaatctgta tttaatgaag aaaatttaaa
     1021 aaaagcctat ggagatacta tatttattgg agagggggaa taatatgctt caaaatttta
     1081 ttaatggtct gtatgacttt cactttttac agaatgctct aattacatct gtagttatag
     1141 ggattgtttc tggaacagta ggttgtttta ttattttaag aggaatgtct cttatgggtg
     1201 atgctatttc tcatgctgtt ttgccaggag tagcaatttc ttatatttta ggtattaatt
     1261 tttttattgg tgcgattgta tttggtctat tagcttctat cattattact tttataaaaa
     1321 gcaatagtgt aataaaagga gatacagcaa ttggtattac tttcagttct tttttagcac
     1381 taggagttat ccttatagga gttgcaaata gctcaacgga tttatttcat attttatttg
     1441 gaaatatttt agctgtacaa gatattgata agtggattac aattggcgtt tcggttttag
     1501 tgttggttat agtagttcta ttttttaaag aattattaat cacttctttt gatccattaa
     1561 tggcaaaagc aatagggatg aatgtcagtt tttatcatta tctattgatg gttctattaa
     1621 ctttagtagc agtaacagct atgcaaagtg tgggaactat attaattgtt gcaatgttaa
     1681 ttacaccagc agctacagca tatttatatg caaatagctt gaaaactatg atatttattt
     1741 ctgcaacagt cggagcaagt tcatcattgc ttggattgtt tattggttat agttttaaca
     1801 tagcagcagg atcaagtatt gttttaacag cggcattctt atttgtaatt agttttctaa
     1861 tctcaccaaa acaaaaattt ttaagaaaga aggagagatc gttatgatga gaaaatggaa
     1921 agcagtactg ggaagtctgg ggattttgat tgctcttttt atattcgggg catgttcaat
     1981 aaatagtaaa gacaaagata aagtggcttc gaacgaaaaa ttaaaagtag tagttactaa
     2041 ttcgatttta gcagatatta ccgaaaatat agcaaaagat aaaattgatt tacacagtat
     2101 cgtacctatt gggaaagatc cccacgaata tgaacctttg cctgaagatg ttcaaaaaac
     2161 ttcaaaagca gatttgattt tttataacgg tgttaacttg gaaactggag gaaatgcttg
     2221 gtttacaaaa ttagttaaaa atgcgaacaa agaggaaaac aaagactatt ttgcagcaag
     2281 tgatggcata gatgttattt acttagaggg tcagagtgag aaagggaagg aagatcctca
     2341 tgcttggtta aatttagaaa acggtattat ttacgctaaa aatattgaaa aacagttagc
     2401 ggaaaaagat cctgataata aaaaattcta taaagaaaat ctagataagt atattgaaaa
     2461 attggattct ctagacaaag aagctaaatc taaatttgct tcaattccga atgataaaaa
     2521 aatgattgtt acaagtgaag gatgctttaa atatttctcg aaagcgtata atgtgccttc
     2581 tgcttacatt tgggaaatca acactgaaga agaaggaaca ccagatcaaa taaaacactt
     2641 agttgaaaaa ttacgcacaa caaaagttcc ctccttattc gtagaaagca gtgtggatga
     2701 tagaccaatg aaaacagtat caaaagatac caatattcct atctattcaa cgatttttac
     2761 tgattcaatt gcagaaaaag gacaagatgg tgatagttac tatgcgatga tgaaatggaa
     2821 cctggataaa attgctgaag gcctttcgaa ataatttgga gggtaggtat ggtacaaatt
     2881 aaaacgacta ctatactaat ttatttacta gcaattcagg aattatcaaa aagaaaaaag
     2941 aaattaaaaa ataatgattt agcaaaagtt ctaagtgtta gtccagcatc tgtaagtgag
     3001 atgctggcta aacttagata tgaggactat ttagatatag gatttcaatt aactgataaa
     3061 gggaaaaaat ttattgacga ttacaaaaaa ggttttaagt aaaagacttt atttttaggg
     3121 aggataagaa aatggctatt ggagtgatta tgataggact tatcctaata gctggatata
     3181 atttttttaa atctttctcg gattatttta gaaaaggtga atgatttaga tgagtctatt
     3241 aattcttgta ggattgcttt ttgtatcagt tattctgctc tttctcatta gtttagctct
     3301 aaggaatgag ttcagtaata aacgttaacg attgaaactt caagtcctgt gtaagataat
     3361 aaagttaatt catcttatta ggaggaagta cccatagaaa ccagagatta tattataact
     3421 caccaaaagt taatagaaag tggactaagc cattttgaaa aagaaggatt tcagcaaact
     3481 aagataaggc aactgtgcct gaccgcaggc gtaactacgg gtgcttttta taaacatttt
     3541 acttctaagg aagcgttatt tgaagaaatt attcagccgc atattaatgc gcttattaat
     3601 atgtataatg aagagtttac caaatacctc ttaaacttag aaaa
//
LOCUS       DARDHA010000201         3609 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000201 DARDHA010000000
VERSION     DARDHA010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.205"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            19..2016
                     /locus_tag="SDA10_002634"
     CDS             19..2016
                     /locus_tag="SDA10_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241503.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(2092..2427)
                     /locus_tag="SDA10_002635"
     CDS             complement(2092..2427)
                     /locus_tag="SDA10_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4241504.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            2554..2769
                     /locus_tag="SDA10_002636"
     CDS             2554..2769
                     /locus_tag="SDA10_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4241505.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            2806..3168
                     /locus_tag="SDA10_002637"
     CDS             2806..3168
                     /locus_tag="SDA10_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEG4241506.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            3214..3540
                     /locus_tag="SDA10_002638"
     CDS             3214..3540
                     /locus_tag="SDA10_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241507.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT         1304 a          472 c          724 g         1109 t
ORIGIN      
        1 tataaggagg aaacacaaat ggaaacatca atgattaata ttccaattta tgtagatcaa
       61 gaaaaattat tagagaagcc agttacaaaa aaagaaaaaa ctgatgtaag agataggtgt
      121 ttagataact atagagaagt tacaagagat gagtttgtag agatactaaa ttcatcacaa
      181 tcatttattc caagcaagac aaagaataaa ggcaatgaca aggagagttt tgtggaaacc
      241 agagtaatta ttctggatgt cgataacact gttaaagatg agaaggggaa ggtagtagat
      301 ttgagtaaag atgatagtag atacttaagc gtagaaaagg ttttatctat caatttagtt
      361 cataattctg cgtttgcgat acaaaaaagt attcggtact ctgaaaatct agaaaaatac
      421 aagattgtct ttctattaaa agaagcaatt actgactatg gtaaaatggt tgctgtttac
      481 gaatacttga aaaagaacat ccccgggtgt gatgacaatg taaatgcttc gaatagaatg
      541 ttttttggag gatataagtc agatgcagtg ataataatta atgaagacaa catgttagat
      601 attactgagc ttccgattga ttttaccatg acatgttcta aaaattgtca aagtgattca
      661 gggagagagt atgaactagt caatgaaacc gattttgtta agttgataaa aaatgacgac
      721 aaagaagaaa tgaaacagtg gtttaaagac tgcctgtttg attcctccga catgtctttt
      781 aatgagattt atgaaagatt gttaaccatt gatatgaata aattacttaa gtcaagtaaa
      841 aacttaagat gtctgtttca caacgatcat aatccctctg ctagtatttt taccagtaaa
      901 actgggcatt ccatttttta ttgtcattcc agtaattgtg atatcagtga aaattttatt
      961 ggagttgtga tgctaatttt aaagaaagac agtcgggtag aaacatttaa ttggctatta
     1021 gagaacctag acttgtatcc tacacatttt cgaaaattga aagaggaatc aaaagtactt
     1081 tttgacactc ttgaatcaat gaaagacaaa cttttcagga caataaggaa ttatctagag
     1141 gaaatgcgac gtgtctatga tgtcttgctt tctgatgtta atttttttga tgatagtaag
     1201 gaaagtgtga cttgtatttt gtcgggggaa caattagcta aaaaaatgag ttcttactat
     1261 ggtaaaactt atgacataga taaatgtaat aagttactat cacttatgac ttttatagga
     1321 ttgataaaaa aattagatga tgaagatgtt cctttgaaga tgctcgaata cctaaatagg
     1381 ttaaaggaag agaaagcgtt aggaaacttt aatcataaga gaagcaatgt ctatcgatta
     1441 gacgttttta atgcaacttc tgtgattcaa aaattgaatg aagatatcct tccacttctg
     1501 gaaaaaattc attttacata tcaatatttt tcttatgaat gggttaagat atgttttaac
     1561 gaaaaagagg ctaaaagagt atttcctcaa aattcccgat cagaactcag caaggaaaaa
     1621 caattgattt tagatgaagc acatgaagtt ttacagggct tatcgtggca agggacatat
     1681 ctaatgacag agaaagaact tattaacact gtttgtgaac gcctacgtaa tgttggggat
     1741 aacagcgtga aaaatgcttt aataaaaaac agaggctact ttgttaaaca aggattcact
     1801 cttgaacgag cttctaagga tattaagaaa ctatacaatg tgaaagcaag tagtagtttt
     1861 ttaatatatt tagcgaaagg ggcagggata gatgaaacaa taaaagaatt gatattatca
     1921 ggagctggga tagttcctct gtatcaattt acaagtgtaa caagagatga tggacggaga
     1981 gtaaggaaga gaaaagaccc aattgtatta aactagacag gttatccata gctcttaatc
     2041 ttatttcatc tcaatacacc caccgttaaa gttcggtggg ttatttattt ttcaatcttt
     2101 caatagctga ttgcgttcat tttcttgcct ttttaatagc ttcatatagt caattgttcg
     2161 aactgcatct tcagtgctaa gagaacgata gagagccaaa aactcttcct cataatctac
     2221 gacaacattg tctagaaaat ggcttacagg ggttcctagg gctctagaaa gagctgttaa
     2281 gctatcgagg ttaggctctg ttacagaatt ttcccatctt gctactgcct gttgtgtaga
     2341 gaagattttt tcagctaatt ctttttgtgt ccacccgaga gcttttcgct tttctctaat
     2401 gacctttgca tattgtttct gtttcattaa tacaccccca tctcagtata gtacaacaag
     2461 ttgttgtatt tgtaatggta tttgaagttt tttatatttt atgagttgaa atacatcata
     2521 ttgttgtata taataaaaca ggaggggaga atcatgaaaa aagtaagtaa tctcagccga
     2581 tacagaaaag aaaaaggact ttctcaaacg gagttagcaa aaaaaatgaa tgttacacag
     2641 caatgtgtaa gttcgtggca aacagggaga acgattccta aaccctatca aatgaagatg
     2701 ctttctgaga tattaagtgt accaatgaat gagctgtttt ctgatgtatt taataaagta
     2761 aacagttaac taataagcaa ttgaatgatt ttaggaggag aagacatgta ttgtagaaaa
     2821 tgtggcaaag aaataacaga tgaaagtaag tattgccagt tttgtggagc actacaagaa
     2881 acagatgaga caaccggaac agacacagta aagaaaagta ctgagcagac aattaatgaa
     2941 atgaaggaac aaatcgtaca tggacaagag acagggaagc tgtatttgat tattggttgg
     3001 atttctatgg tagtatcgtt agcatttatt cctgttgtat ttggtgctgt tgctgttatt
     3061 atgggctact tatatcgaga aaaagatgag aaaatgggta cgattttgat gatagcaggg
     3121 gtagctggag caatctttgg tgtactttta gggatgtcaa tgtactgata agagggataa
     3181 gagcaaatta ttttggagat aggagataaa aagttgaata aagtaaatat gaacaagaag
     3241 tttgtcatta taggtattgt tgtcttggct attataggta ttattttttc aaaaatgaca
     3301 ggtacatctg gattagagaa gtcatcaaaa ccattggtaa cacagatttt acaagaacag
     3361 tacaacttaa atcgatcatg tgatgatgtg acgattactc atgaaaatgg agataacaca
     3421 taccgggcta aagctatatt agacaatgga agtgctatca acataaatat tgagtactat
     3481 ccaaagaaag accgtatata tgtggagata ccatatgctg aggtgttaat gttaaattga
     3541 tttaaatgta ttatgcatac cgtccaaaca ggaactccca aaggtgtaaa ttcactaggg
     3601 agtttttat
//
LOCUS       DARDHA010000202         3566 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000202 DARDHA010000000
VERSION     DARDHA010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.206"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(133..717)
                     /locus_tag="SDA10_002639"
     CDS             complement(133..717)
                     /locus_tag="SDA10_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG4241508.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(928..1131)
                     /locus_tag="SDA10_002640"
     CDS             complement(928..1131)
                     /locus_tag="SDA10_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241509.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1141..1563)
                     /locus_tag="SDA10_002641"
     CDS             complement(1141..1563)
                     /locus_tag="SDA10_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241510.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1801..2415
                     /locus_tag="SDA10_002642"
     CDS             1801..2415
                     /locus_tag="SDA10_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG4241511.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2580..3404)
                     /locus_tag="SDA10_002643"
     CDS             complement(2580..3404)
                     /locus_tag="SDA10_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241512.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT         1177 a          623 c          516 g         1250 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      721 aatcactcct tcattaattt tattgtaaca taactttatc catatagttt aactgaacat
      781 agatattgtt atgggtttat gaacatgaaa tgaattgttt ttttgaaaaa taaagcaaaa
      841 aaagaaagtg tccagaaatg ggtgatttta tgaacacttt caattagaaa aaaagaatgt
      901 cgactagaca ctcttttttt aaaatcatta tttttctttt tgatatgttt tttggtctct
      961 tttttttctt ttttcttcag aatcttctag attagaaatt gatttttgaa ttttttgtgt
     1021 ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat taattttact
     1081 tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca taaaaaatcc
     1141 ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag ctgatttata
     1201 actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt tccaattccg
     1261 tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc ctgtcactcg
     1321 tatcgtttta gaagctgcta gagcaagtaa tggagaaact attaaataat tttcagtaac
     1381 agttaagtcg ttagttgggt taccaatggc tattccgtcc ctatctaaag gagttaattc
     1441 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
     1501 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
     1561 cattgtctat cacctcgaaa tcaaaactaa gaaaacgttt tcttaataaa tcttaacaga
     1621 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
     1681 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacata
     1741 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
     1801 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
     1861 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
     1921 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
     1981 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
     2041 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
     2101 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
     2161 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
     2221 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
     2281 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
     2341 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
     2401 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     2461 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     2521 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     2581 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     2641 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     2701 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     2761 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     2821 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     2881 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     2941 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     3001 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     3061 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     3121 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     3181 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     3241 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     3301 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     3361 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     3421 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
     3481 ttttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
     3541 tggggagtac ttctttcaaa ttcaag
//
LOCUS       DARDHA010000203         3564 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000203 DARDHA010000000
VERSION     DARDHA010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.207"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            577..909
                     /locus_tag="SDA10_002644"
                     /pseudo
     CDS             577..909
                     /locus_tag="SDA10_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
     gene            923..1072
                     /locus_tag="SDA10_002645"
     CDS             923..1072
                     /locus_tag="SDA10_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241513.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1069..1515
                     /locus_tag="SDA10_002646"
     CDS             1069..1515
                     /locus_tag="SDA10_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241514.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1517..1654
                     /locus_tag="SDA10_002647"
     CDS             1517..1654
                     /locus_tag="SDA10_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEG4241515.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            1691..1984
                     /locus_tag="SDA10_002648"
     CDS             1691..1984
                     /locus_tag="SDA10_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241516.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            1981..2205
                     /locus_tag="SDA10_002649"
     CDS             1981..2205
                     /locus_tag="SDA10_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEG4241517.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2202..3221
                     /locus_tag="SDA10_002650"
     CDS             2202..3221
                     /locus_tag="SDA10_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEG4241518.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            3410..3483
                     /locus_tag="SDA10_002651"
     tRNA            3410..3483
                     /locus_tag="SDA10_002651"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3444..3446,aa:Ile,seq:cat)
BASE COUNT         1225 a          638 c          741 g          960 t
ORIGIN      
        1 ttactagatg acccttactg ggtaggtaaa acatcgttgg gtgagaatat tgcaaataaa
       61 ggtacgacgt tcccaattaa atctagcgcg tacgaaatat ataaaggtaa cacggaagaa
      121 gagcttataa taggtcaaac gtatactatc acgcttaaag gaacaaaacc cgcaagtcaa
      181 acctttgtag catataatta ttggaacgtt aattttggag aactaaaacc agttgaggga
      241 ttgacagacg tatggtctct aacattcaca ccaacgaaac ttgaaccggg tttgcctaaa
      301 gaccttcgca tttttcagtt gcctaaagaa acggccggcg catgccaaat tgactggctc
      361 aaaattgaaa aaggcgacac ccgaaccccg aatattagtc agtttaaata ctttggtgaa
      421 ggattgaaag atagcaacaa tccgaacgac tacagctggg acatcacgcc tgaatatact
      481 gaaaaaggct tgaatgatac ggttagtttg actgaaccgc aatccatcga aggattgaaa
      541 aatttcgaag atggtattca atcaaaagga aaatctgtat tgacatcaga cgacaacaaa
      601 tatgaagtcg taaccttaac agttacaaac gggaataccg gatcagcaaa gctttatcgt
      661 gaaggaaaaa ccgtcactat ttattttttt gcgttgaatg ggaaaagcag tggtggaaat
      721 gattcaacga tactaacaat tccagaaggc tatcggccac caattagttt tgagcaactg
      781 gttggctcga tagaccgttc tactttgaac agtgctcagt tatctattgg tgcagatgga
      841 gccattaaat ggcgaagaaa ctcaagttat ggatcagctt attcatttgt tatcacttat
      901 tcaatttaag ggaggaaatc taatgaaagt agtttacaaa tcaatcaagc cttacggatt
      961 cgagcaaatc attgtgaatg acgaagaaca tttaccggaa gaatgcacag aagtcgaacc
     1021 accgattcca aattggaaac cgaaattcaa ttactgggag ggaaataaat gaaaaacatt
     1081 tggaaatatg gccgtactgg cggagagtat gcaggaaaag tattggacga catgcttgta
     1141 tccgttcctt acacagatca gccaccgctc gaaggggttc gttcagatgg cgaaccgcta
     1201 acgattgctg atcagatgtt tgatcctaaa ttgaaccaat ggattatttt agcgaacgcg
     1261 ttagatcaca acgatttaaa caatctcaaa gcgatgtacg aggctcttga acatgaaaac
     1321 ggcaacctaa aacagctaaa tgccaaactc atgctaagcg atgtagcgat taaacaggaa
     1381 aatactgcat tgaaagaaaa agctgacagt ttagcacaaa tcaattcaaa aatgatgctt
     1441 gcttcgttac aaaatagcaa agacatttca gaaattaaag agcaactaaa tccagcttca
     1501 aaaggaggtg agtagtatgt ttagttttag cgatgtgaaa atgatgtatg attggggctg
     1561 ttttactgac gatcaagttc gactattcgt tccactatgc attacagacg aagaagcaga
     1621 taaaatcatt aataaagata agagcgcatc ttaattgatg cgtttttatt taaggtaaag
     1681 gagttgtcac atgattaatt taggggaatg gggaatgata gcaggatcaa taaccgctat
     1741 cgtttctttg attttattag taattagacc gattgctgca tctttctcga aaattactga
     1801 gactctttca aaagtaagcc acaatttaga tttgctgact aaagatttag aatcgagcaa
     1861 atcagatcga ttgatgattc atgaagaact aaagaaacac gatgaaagat tagatacaca
     1921 tacagaaaaa ttggtggaac acacgcaaca aatcaaaact ttgtttaggg agagaagaaa
     1981 atgaataata aaacgttcga agtactaaaa tggttcgcac tggtaattat tcccgcacta
     2041 gctactttcg tggggttagt tggtaaagcg ctcaattggc agtacacaga tatctgtgtt
     2101 gtcatcatta ctggttttgg cgcgttttta gggagtgtgt tgggtgtatc aaatcgaacc
     2161 tacaaaatgt tctcggctga aagcgaagaa ggaggaaaca aatgaaaaag aaaattacta
     2221 ttactgcgat gagcctgtta acggctcttt ttttattgcc aattaacgga tttgcctata
     2281 ctatcaacaa tgaatttaat ttgggcgcaa atgaaggtag ctcacaagta gcaaataatc
     2341 agtatatttt actgcatgaa acggctaatg aaacagcaac aggacgcaat gaagcgcagt
     2401 atatgcaacg ttcatggact agcgcttata ctgcttatat tgtgggagac ggcggaattg
     2461 tttaccaagt cggacaacct ggttatgtac agtacggtgc tggttcatat gctaatgcca
     2521 atagtcctgt gcagattgag ttacaacaca cacatgataa agcaactttt gaaaaaaact
     2581 ataaagcata cgttgaattg gctagagatt cagcaatgaa atatggtatt ccattaacat
     2641 tagacacgcc ttataaccaa ccaggaatca aatcacattt atgggtaact caaaacattt
     2701 ggggcgatca cactgatccg tatggatact tatcagaaat gggtgtaagt aaggaaaaac
     2761 tggcttacga tttagctcat ggatttacag acgaaaaccc aacgacttct gagaacaagc
     2821 ctgtcattga tccaacccga gcaggtgcag caaatccaac tttatcagat ggaacgaacc
     2881 attctcacat tgatcagttt ggagaaatcg aaaatgcgaa cttacacgtc gctggatggc
     2941 acatcgctaa ctataagtac gagtatattt tcattatgga ctacaatact gggaaagaat
     3001 tagctcgagt aagagctgat ggaatttata gaccggatgt aaatcaagct tataatactt
     3061 ctggaaatgt tggctatcat gtatctttta acatgcgtaa cttccctaat aagaaagtct
     3121 atgtcatgat gcgggcaacg aatgatccag agggaaacac taaaggcggt gcgcaagatt
     3181 tccatgacaa acgttggtat ttaaatattc ctaaacgata aaaatagctc ctcgttgagg
     3241 agcattacat aactatattg acaactataa aaatcattcg ataaaatagt gatgttatcg
     3301 catatcttca ctatcaccca taatagtcac actccaagct atgcgataac aggtttgttg
     3361 ccacacattc tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc
     3421 tcagttggtc agagcagacg gctcataacc gtccggtcgt aggttcgagt cctacagggt
     3481 acattaacgt agccatttga atcgttctgt gttagaattt tttgaagagt attatacaag
     3541 ctaaagcttt tcttcattgc cact
//
LOCUS       DARDHA010000204         3461 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000204 DARDHA010000000
VERSION     DARDHA010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.208"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(39..1238)
                     /locus_tag="SDA10_002652"
     CDS             complement(39..1238)
                     /locus_tag="SDA10_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG4241519.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1368..2744)
                     /locus_tag="SDA10_002653"
     CDS             complement(1368..2744)
                     /locus_tag="SDA10_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4241520.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2744..3400)
                     /locus_tag="SDA10_002654"
     CDS             complement(2744..3400)
                     /locus_tag="SDA10_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4241521.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
BASE COUNT         1055 a          649 c          466 g         1291 t
ORIGIN      
        1 taagcatcgc aaattgattt ctaccattcg tattgttttt agacatcata gctatcactc
       61 cttgcattcg ttttcataaa tctattataa aagaaaagac tgtagacatc atccataaaa
      121 tggactttgt ctacagtctg cgcattctca ttagagaaca ctcccttttt tgtgactttt
      181 ctgaagctag agtgaatact ttcaactgca ttagtagtgt acatgatttt tctaatggcg
      241 ctgccataat caaagagttg ttcaacatga gaaaagtttc gtttgcatac atctactgca
      301 ccagaataat gagaccaccg attttgaaaa ctgtcgaagg cagcgtgtgc agcgtttaaa
      361 gaagaagcac tgtagaattt tttcatgtct cgacagactt ccttataatc cttacttggg
      421 aggtatcgta acgcatttcg aacaagatga acaatacagc gctgcacaat gacagaagga
      481 aaaattgctt ttgcgccttc tttgagaccg gaaacaccgt ccatcgaaat gaaaaaaaca
      541 tcttctacac cacctacttt cagttcatca aatacttgca tccagcgatt tttagattct
      601 gtttgattga gccataggcc taaaatctcc ttgtttcctt taagatcata gccaagaatg
      661 gtgtacacag catattcttt agcttcatag ttttcgcgta aagtgacgta catacaatca
      721 acgaatagaa atgcataaca cttggctaat ggccgagctt gccattcttc caattcagga
      781 aggacggcat ccgtgatatc agaaatcatt tcgtgggaaa tatcaaagcc gtaaatatct
      841 tcaacggttg cggcgatatc gcattgactc attcctcgtg catacatgga aagaacctta
      901 ccttcgatgt cggagacatc tcgttttcta ttaggaatca actcaggttc aaaagaagcc
      961 tcccggtctc tgggaacatc aatgtttact ttaccaaagc tcgttttaag cgtcttattg
     1021 ccataaccgt ttcgtcgatt gtcatgtttc tttggctctt tagaatgggc gtcataccct
     1081 aaatggttat tcaattctcc ttgaagcatt ttttcaaata agggaccaaa cacatctttt
     1141 aaaacatctt gcatatcctc tacagattcg ggttgataag tattcaaaat ggattcagct
     1201 aacttctcag catcaggatt tcttttcttt ctagccatcg tgtaatcacc tcttgatctt
     1261 attttagaaa aataaaaacc gtgaagcaac cacccgccta ggattgatgg ttacttacac
     1321 ggtttatatt acactctcat ttttggtgct aaaaagcacc gaaaatttta attattgtca
     1381 attaataatt cttttggatt ccttcttaat atattgaatg aagaaataat caatgataca
     1441 agaagtacta aactcataaa tataactaca taaatcatga atttaggtaa tacattgata
     1501 tctaaacttg ataatgtctt gttaaatcct tcagcttctg ccccaccacc taattggcta
     1561 gatgcagatt gtttagctat ttgtttagct atatcaccag taaccttatt caatatatta
     1621 ttcccaagct tgtcagctgt atattgagct aagaaatagg aaccaacgaa tgcggggatt
     1681 gatataaata ccatctcaat taggaattga ccaaaaattt ctaattttga tatacctaat
     1741 gatagtaata ctgctatttc tttctttcta gcattcatcc ataagaataa taataatgaa
     1801 actacaactc ctgcaaatat taatgagcca aaaaataatt tatttgaaat tgagtaaata
     1861 cctgatatag attgttgtaa tgcagggtaa tttgatgaac ttttaatcaa attatattct
     1921 ctccaattta tatctaattt cccaagatct tttattacac tatcaagatt cttatcacct
     1981 tttacaaaga atgttgcatc ttggtataca gctgtatctt cggtattacc ataaacttta
     2041 gcagctgtat gaatatcagt gattaatgta ttttcgtata gttcttgtgc tgcacttact
     2101 ccaccgctat tatgcccatc gaatagacct ttaatttcta cttctactgt ttcatctgca
     2161 cctttctcat tatcagcatc aaataaatta gattttattt ttatcttatc tccaacttta
     2221 aggttatttt tttcagctaa atctttatgc attaagattt tatttttatc ttcattttct
     2281 aaatgctttc cttctactaa tttatatgct tctgatacaa attttgtttc ttttgttgag
     2341 tcgttaaccc cagttaacat aactgttctt ttaaaattct ttgctctttc aggtgattga
     2401 ttcgcaagag tctcttgagt ttcaataata tcataatcaa ctaaatctgc aacactgttt
     2461 attcttttta cataggagtc tatactatct gtttgagata tctttttaat atcttcacct
     2521 tttacatttc ccccacctct aggtgttcct ggatttactt gtctatttat ctccatagaa
     2581 aaactatttg ttatattagc aaatgtttct tttgaagctc tgtccgtagc atctttaata
     2641 gataaactaa tgagacttaa agttgacata gataaaataa ctaatatgat aattaatgat
     2701 tttaaacttt ttctagttac ataagcaaat gcatttttta tcattattca acctccaaat
     2761 tcattttatt tacttttttc aactttttac cacttagttc taaaatgata tccgcagaat
     2821 ctgctacttc cttactatgt gttacaacta ttacacattt atttctatct ttagctaatg
     2881 tctttaatat attaattatt tctccagcag taacactgtc tagattacca gtaggctcat
     2941 cagctagtat tattggagca tctgatacca atgctctagc aatagctact ctttgttgct
     3001 gtccaccaga taatttcata acatttcttt ttatttgttt tttatctaaa cctagttcaa
     3061 acaagatact ttcatctgct gatttattta ctaatctaat attttcaatt ggtgataaat
     3121 aatctattaa attatagttt tgaaatacta aagatatatt attttttcta tgattactat
     3181 atcctttatt ttgtatatct tcattcttaa acaatatttt tcctgtttga ggtttatcaa
     3241 gtccagcaag taaggaaaga agtgtagatt ttcctgttcc tgactttcct actatcgcat
     3301 aaaacttccc aagttcaaat ttttgattta ctcctgacaa aactttttct ttagaatttg
     3361 cataactata tgctacattc tttatttcta atatatccat tattttctcc taacttattt
     3421 ttgataagat ttctttaggc ttcttgatta acattaatga a
//
LOCUS       DARDHA010000205         3450 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000205 DARDHA010000000
VERSION     DARDHA010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.209"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..753)
                     /locus_tag="SDA10_002655"
     CDS             complement(<1..753)
                     /locus_tag="SDA10_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HEG4241522.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDM"
     gene            complement(759..1865)
                     /locus_tag="SDA10_002656"
     CDS             complement(759..1865)
                     /locus_tag="SDA10_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEG4241523.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            complement(1865..3001)
                     /locus_tag="SDA10_002657"
     CDS             complement(1865..3001)
                     /locus_tag="SDA10_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG4241524.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(3033..>3450)
                     /locus_tag="SDA10_002658"
     CDS             complement(3033..>3450)
                     /locus_tag="SDA10_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEG4241525.1"
                     /translation="ENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDE
                     FPQFYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFE
                     EVVKLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
BASE COUNT         1054 a          708 c          388 g         1300 t
ORIGIN      
        1 catatcatta tttttccaat aatttaaaaa aaagtctcta attatagcaa acatccatct
       61 agactcttga ttacatgcta atgaataacc cgcaaaatat cctgatgcta cggatgcgtg
      121 agcataatca ggcctcctaa tagaccaaat atcgatatca aaatactcat ctggtattgg
      181 ttttgtacaa taaaatgtgg cgtctaacca caaacctcca tgttttgcta gcaaagataa
      241 tcttaataaa tcagaataat ttgttctcgt aatgattcct ttgtttttct tttcttccac
      301 ccattttggt atatcaacat attgtctata attatcttct gttaaaatta taacagtatg
      361 tttccctgca tttcgtttta ttgattttac acaagctttt acaatttccg gcgattgatc
      421 caatccctgc caccaacaaa cccaaatgca gttagaatat ggtgattttg attgattaat
      481 tatttgattt tgactataat catatgtttt caaaaaatct gaaaaagtct tttcataata
      541 cttcagcatt acctcatgtt tttttaataa atgttccttc tgttttttac tttcattata
      601 tccattacga ttcatgactt gaatatcaat tttagcttgt aacgtggcaa atgcttcctt
      661 ccaagatgtt atctttgcaa ttcgtcttgt tgcattcaat tcttcggata tgcgatgtat
      721 aattcttttt atacttgaat gtttctgtgt catattcttt aacctctttg tattaattca
      781 aataactcat aatattttga ttctaagatt ttgtatgctg tttgaatatt ataatctgcc
      841 tttattaaaa tcgaacgttc tgaatctcga tgagagatat ctacatttgc aatctctctc
      901 gcccattctt ttgccgattg atttaaactt atgaaattgg tcttatcact gaaagcaact
      961 tctgtaggaa ctttctccga aaagaaacac ggtaaatctg caaattgagc ttcaataccc
     1021 actaacggaa ccccttcaaa taatgatggc aacattaata cgtcaaatgc ttgataaaat
     1081 ttttctatat catttctatt tcctaaaaat ttaatatcat catctatgcc acttttcttt
     1141 gcataatttt ttatttcttc ttccatatca ccagttccaa taattaataa tacggaatta
     1201 ttcttaatag cgtgaaattc tttaaaaatt tcaatcaaaa aacgatgatt tttagaatat
     1261 gaaattcttc caatatgtcc cattacaaac gtctgacttg aaattcctaa tttcattcgt
     1321 acatcatttc taatactttc atcataataa aattttttag catccactgc atttggaatc
     1381 aaagtgaatt tatcatttcg aaacaaaaaa tcacctgcta cttttccaca tgaaaattca
     1441 tgtgtcaaaa cagacgttaa cttacatcga taatattgct ttaaaagata tttaaaatct
     1501 ctatctatcg atgtattatg gctatgtgct atccgtatcg gaatcccagc tttttttgca
     1561 gcttttaaag gtaaataact catcgcatca atatgagaat gaacaatctc atattctgga
     1621 tgatccttaa aaaattgttc catccattta aaatatttag gataattttg tggataaagt
     1681 ctcggtgcat aatagacctt tcctccaaga cttattattt cctcatcata atcactttta
     1741 tatggacgat gtgtcaaaaa atcaaactga attttagttc gatctatatt cctataatag
     1801 ttcattagca tcgtctccaa ccctgcacga tgcatatcat tcacacactg taatattcga
     1861 atcattatat atttccccta tacaaattta atgtttcttg tacgatttta tcccaattat
     1921 atttactaca aataaactca gtagcttgat tcttcaaaac tttaaccatt tccgattgat
     1981 tgcatgcctc ttctaatctt atctttaaat cacttacatc cgatttctta aaaatcatag
     2041 ccttgtcttc tactacttcc gtacactctg agatatttga aactatacaa cagttcccat
     2101 aactcattgc ttctaacaaa cttaatggca ttccttctaa atcacttgga agtgtataga
     2161 tatacgcatt actatataat tcttctaatt cctgtccttg tacaaatcca gtaaatataa
     2221 ttcgctcatc accttttgct aattccttta gttcatttgc aaattcatct gtatcagagc
     2281 ttcctccggc tattataagt tttttatctg tctgaacatc tttaaatgcc tcaattagat
     2341 accgaattcc tttttcggga accaatcgcc ccaaaaacaa aatataagaa tcttttgtta
     2401 atccatactt ttctgtaatc aatcctgctg attttatttc aattttttta acgccatttg
     2461 gtataaacct tgtttttctt ccatatatat cttcaaaata attttggacg ctttcactaa
     2521 gtacaattat ttcatctgca aattttacag cacacttttc ccctaacatg atgtatgtac
     2581 tagcaagctt gttccatttt tctctttgat gatctagacc atgaattgtt gcaacacatc
     2641 ttttcccaaa aagttttggc aaccacaaca tagcacatgg tccttctgca tgaaaatgaa
     2701 caacatcata ttttccgaat gctgctctaa ttccaccaaa tactgaagaa ctcattgctg
     2761 caattccttt tatatttaat gtaaaaatag tctttaattt tacaccttta tattctttat
     2821 ttttatgctg gtcaaattcc tttccgctaa catgatgacc acttcgatta tagcaagtca
     2881 cttcatgccc aagtgcaacc attcgcgttg ccaattcttc taccactatc tcaattccac
     2941 cttctcttga tggaattctt ttatgaccta gcatacagat ttttaatttt tttcttgcca
     3001 tcacgtttta ctccttctat agtatcttta tcttacatgg aaccgtttct atgaagcaca
     3061 gttttgactg tcctaaacaa aattctaaaa tctaatccca tactccaatt cctgatatat
     3121 tctgcatcaa gttttacaac ctcttcgaag tctgtgatat cacttctacc actcacctgc
     3181 cacattccag taatcccagg tttcatcgca attctcgcac gatgatgaaa atcatacttc
     3241 tcatattctg acttcaagca aggtctaaac cccaccaggc tcatttttcc cattaggaca
     3301 ttataaaatt gtggaaactc atccaatgat gtagatcgaa taaattgtcc aattcctgtc
     3361 ttctttgttc catcaggaag tattttattg ccaatgattc ttgggtcaaa atccatctta
     3421 aacatgagat tattactcat cttattttct
//
LOCUS       DARDHA010000206         3421 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000206 DARDHA010000000
VERSION     DARDHA010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.210"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(220..972)
                     /gene="xth"
                     /locus_tag="SDA10_002659"
     CDS             complement(220..972)
                     /gene="xth"
                     /locus_tag="SDA10_002659"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEG4241526.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="SDA10_002660"
     CDS             1085..2005
                     /locus_tag="SDA10_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG4241527.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="SDA10_002661"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="SDA10_002661"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEG4241528.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
BASE COUNT         1193 a          574 c          684 g          970 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agaggctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 a
//
LOCUS       DARDHA010000207         3298 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000207 DARDHA010000000
VERSION     DARDHA010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.211"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..2232)
                     /locus_tag="SDA10_002662"
     CDS             complement(<1..2232)
                     /locus_tag="SDA10_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241529.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="SDA10_002663"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="SDA10_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          900 a          739 c          535 g         1124 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg aatccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattccc
//
LOCUS       DARDHA010000208         3221 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000208 DARDHA010000000
VERSION     DARDHA010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.212"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            301..585
                     /locus_tag="SDA10_002664"
     CDS             301..585
                     /locus_tag="SDA10_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241530.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            588..1004
                     /locus_tag="SDA10_002665"
     CDS             588..1004
                     /locus_tag="SDA10_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241531.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1141..1860)
                     /locus_tag="SDA10_002666"
     CDS             complement(1141..1860)
                     /locus_tag="SDA10_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG4241532.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLE
                     FTFSSLTSDLPKISLRNWLEE"
     gene            2535..2630
                     /locus_tag="SDA10_002667"
     CDS             2535..2630
                     /locus_tag="SDA10_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG4241533.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(2932..3177)
                     /locus_tag="SDA10_002668"
     CDS             complement(2932..3177)
                     /locus_tag="SDA10_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241534.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
BASE COUNT         1074 a          599 c          503 g         1045 t
ORIGIN      
        1 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
       61 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
      121 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
      181 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
      241 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
      301 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
      361 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
      421 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
      481 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
      541 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
      601 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
      661 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
      721 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
      781 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
      841 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
      901 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
      961 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     1021 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     1081 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     1141 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     1201 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     1261 atcattgaag tattttttta gttctcgtaa tgcaggattt aatacttttt gatcaatgtt
     1321 acccatttga tagtattctg ggatatccat taagctccta aactcttcaa tacgaactac
     1381 ataataacca gtagaacggt actgcataag cagtcggaat agagtttttg catacgtaga
     1441 acgcaacgac gtgaaagcag ataattcaaa tctactaaat tcagctgtta aaccattaat
     1501 aatatattct aggtccggat taacactgac ttcaacaaac ttttttgttt tattgatttc
     1561 aaaaccagta aacaaaacaa agtagcgaat agaatcctca tcttcagtac ggtaagtaag
     1621 attaagcata tctttataga gcttttctaa atcaccgaca aaggcttttg tagcagtcat
     1681 cttataatca cttaactctt ttaattcctc gaaagaaaat ctaacttttt tcaaaccacg
     1741 atctttcata cgagcgcata atgcaaaaaa taaatccatc tgcttagcat tgaaattttt
     1801 cagcggtact aaattcaatt cattttgata tttgactgtt aattccgttt tttcatccat
     1861 tcctttcttc cccccttaca aataaataat aacctttaac aaccaactcg taaataggtt
     1921 ggttgttatt atacaaaagg atattagtac tcagataaaa agttgttagt actcagataa
     1981 aaagttgtta gtactcagat aaaaagttgt tagtactcag ataaaaagtt gttagtactc
     2041 agataaaaag ttgttagtac ccttctgtat ccgttgggag agtaaggctc aactgccccc
     2101 taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat aaacgcgcgc gcgcgagcaa
     2161 aatttcccgc caaaaaatca cccacttttt aaaataaaca ggttcgcgca tgatattaaa
     2221 ctgccaatct agctccctat ggtcgctgta agaaggagtg atcgctacgc tctcacattt
     2281 tacttgcagc agtcgccttt aggctccttc gccaaaaata attcattagg gactcctttg
     2341 tggcgtcctg agagctcgtc taagccgttc taagacaaga ttcgataaaa actatgtcct
     2401 tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta acaaccccaa ggcaataaaa
     2461 aacttaggct tatttttgct tttttattgt ataattgatt gtcgcctatc cgacagtctt
     2521 gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat ttgctccctt cttgttgggc
     2581 ttttaagcgc tttgttcagt tactggttga ataaccgaaa caagaagtaa aacttaatta
     2641 aggcgactca gcccacaaaa aaagccccag agaatccgac ctctctgggg ctttcgtgtg
     2701 tttcgtgttc acttatttgc aatcaaatta taacagaaat aaatatttat ttcaaaaata
     2761 tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc aacatttaca aataaagtcg
     2821 ctctgagagc tcgtattttt cgatttaaga cgttttattt agtttttggt gtatttcatt
     2881 ggtccaagct aaaaaactct tagaagcgat tttagagcca tttaaaccca cttaattata
     2941 ttctccatga aattcgctcc atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt
     3001 cactagttgc attaattttt ccaacgatcg ttttctttcg ggtgtcatat ttttatattc
     3061 caacaatttt tcgtctgtta aatcgaactt gattgcttga attgcttgct ctaatttttc
     3121 taagttttga aaatattcgt aaggaaattt ttccattttt tctttctcca gtctcacaat
     3181 ttttctagaa ataaaatcac ttctttgccg tctatactct c
//
LOCUS       DARDHA010000209         3179 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000209 DARDHA010000000
VERSION     DARDHA010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.213"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            612..1523
                     /locus_tag="SDA10_002669"
     CDS             612..1523
                     /locus_tag="SDA10_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HEG4241535.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            2010..2276
                     /locus_tag="SDA10_002670"
     CDS             2010..2276
                     /locus_tag="SDA10_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241536.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2348..2689
                     /locus_tag="SDA10_002671"
     CDS             2348..2689
                     /locus_tag="SDA10_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241537.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2744..3163
                     /gene="ssb"
                     /locus_tag="SDA10_002672"
     CDS             2744..3163
                     /gene="ssb"
                     /locus_tag="SDA10_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG4241538.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
BASE COUNT         1153 a          530 c          687 g          809 t
ORIGIN      
        1 gtatgaaagg cattctccga cttccaggtt tttgaccatt ttctctcaac gtggaaaaac
       61 cgccacttat gagagaaaat ggtcaaaaac ctaagcggga agtctccgaa taacatacca
      121 agcgccgaca gccaacagat aaagaataca ctactaaaaa tagtagtagc ataaaattta
      181 aaagggaaaa aatcaacccc taccccagtc ttggggtctc aaaactaact cccccctaac
      241 cccctctttc agcctctttt gaaagagggg gagaatcaac cccacggcca acgccccagt
      301 gttcttgggg ctgtccagaa cagaaacttg cgagagagtg ggggtaaaac aagtgtagag
      361 gaaacttgaa gacttgttcg aacagaaact tgcgagagag ccggagtaaa acaagtgtag
      421 aggaaacttg aagacttgtt cgaacagaaa cttgcgagag agttggagta aaacaagtgt
      481 agaggaaact tgaagacttg ttcgaacaca aacttacaag agaggggagt aaagcagttg
      541 cagaggaatc aggaagattt gttcgaacag aaacttgcaa gaaagcatta ttaaattgaa
      601 ggtgatgaaa tatgaagtat ttattgcatg gctattttat tgattattct actcgcaata
      661 gtagtacttt agcgattcta tgtttgcttc atgaattgag agtggttact agaaaacagc
      721 tatacaaact attgaatttt gacagatcaa ttagtaaaca aggagtaacg aatgctttag
      781 tgagattata tgaaaaacag ttaatcgatc gaaaaaaata tggaacagaa atgtattatt
      841 atttgacgaa agagggacat caaagcatag gtgggtatta tacgttgccg aaagttcctg
      901 aatataattt gaatcaccat ttacaaatta acaattattt gattaaagga atggcattgg
      961 caaaagacca cccacattta aaacggatta tttcagaaag acgtaccgtc tatgaaacga
     1021 aggatttaaa tcctaagaca aaaggaagaa aattttttgt gccagatttt atttttcgtt
     1081 ttcgagacaa agaaaatcat gagatcaatt ggtcctttga aattgagcta acgttgaaaa
     1141 cacgtcgtcg ctaccgtgaa gcgatttttc cgaaatatat tcgtgagttg cgaacacgtc
     1201 ctgaatcaca tttaatttat gtgacaccta gtggcgcaat tcaagaagaa cttcatgcgt
     1261 ttaagcgtat ttttttcaag cgcgaaactg aaaaagatgc gagtcaacaa gaattgtttg
     1321 agcggttgca tattttttca gctttagagt ttgaagaggg attaaaagca ttattagaga
     1381 atgatccagc agtcaattgg cggacagtag aacgagaaaa aatttatccg aatggcgctg
     1441 ttcaaagtcg tttgaattta tttgaagaat caactgaaaa tgaggaaaaa ccaaaagtga
     1501 agtttacgcc tgaagtattg taaaagaggg tgttcgcttc gctctttttt tgtgccagtt
     1561 tgctggcacc gttcagacgc aagcgtctga accggctcgt ttttaggaag gactaacttg
     1621 tttgatttat ccgttgtcaa agaagtggac gttcgagagt ccctaatcgt atagcagtca
     1681 ttcggcgagt ataacctaaa acataacctg ccaattgcca agggtaaaat gcacgctgcg
     1741 cgcccttggc aatcgtcaga acatgttttc gagccggtta tgccgaacga ccgctatacg
     1801 aaaagggcct ctaaacagaa gtccactttg atccttgaca actgaaaccc aaaatcaaac
     1861 aagcaggcct tcgtctaaaa acgagccgaa tgaagacctt gggggttgca cccccaaacc
     1921 cccgtgcccc cgacacacta ccgaaaaggt gggggtgggt ggcgaaaaaa tcaaataaaa
     1981 cattctattc aagaaaggaa gaaacaacca tgacaactaa aaacgagcaa ctgaaagaac
     2041 aggcaagaaa agagttgcaa gaaagaggtt tgattattga ggggagcttt gaaggagatt
     2101 ttgaaaccta tattggttgt tatgcaagac cgataaacaa acctacagcc ttagacccaa
     2161 tgaacgaaca agaagtaaaa gaacaagaaa aacatgcagt gaatggtttt ccgcaagatt
     2221 tcactgaatg gtatgagtgg gaaattaaca acgggaagct tgaaaacttc ttataaaagt
     2281 cagatgacgt gcaaagagaa tgattctctt tgtacggtaa aaatataaag aaagaaggaa
     2341 aaagaaaatg gcaaaaaaca caccttatca agtaggagac attttttata gtagttgggg
     2401 ctacgaacaa acgaacgtga ctttttggca aattgtgaag ttaacagaaa aaacagcatg
     2461 gtttcagcct attcaaaaga aaacggtcga gcattattcg tccatgagtg gaaaagttgt
     2521 tccaatgaaa gacaagttta tagagtatcc tttagcaaaa actaaagaca gtattgtaaa
     2581 aaaacgaatt gatccagaaa cctatcctga tatgttatgt ggttatcgta gttgggacac
     2641 gtttcataga tataaaaatc aagctgttta tgaaagtagt tattattaag gggatagttt
     2701 cccctttcaa aaataataaa tagaagaatg gagagaaaaa atcatgatga attcagtgaa
     2761 tttagtagga cggttaacaa aagaagtgga tttaaaatac acaccaaaag gcaatgcgac
     2821 aggcacattt attttagctg ttaatcgaaa ttatacgaat gcaaacggcg agcgtgaagc
     2881 agattatatc cgctgtgtca tttggcggaa agctgcggaa actttagcaa aatatactgg
     2941 aaaaggaagt ttgattggca ttaatggacg tcttcaaacg aggagctatc agaatcagca
     3001 aggacagacc atgtatgtta cggaagttct agtgacagat ttctatttgt tagagagtaa
     3061 agaaatcaat gagcaacggt caaaatcaat ggcgaataat caaaataatt cttcaattcc
     3121 aggaaatgag attcaaattt cagatagtta tttgcccttt taagtgaaaa ataattttg
//
LOCUS       DARDHA010000210         3128 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000210 DARDHA010000000
VERSION     DARDHA010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.214"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            103..1347
                     /locus_tag="SDA10_002673"
                     /pseudo
     CDS             103..1347
                     /locus_tag="SDA10_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="SDA10_002674"
     CDS             1360..1992
                     /locus_tag="SDA10_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG4241539.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="SDA10_002675"
     CDS             2197..2424
                     /locus_tag="SDA10_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEG4241540.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="SDA10_002676"
     CDS             2493..2759
                     /locus_tag="SDA10_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG4241541.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="SDA10_002677"
     CDS             2749..3018
                     /locus_tag="SDA10_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG4241542.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttt
//
LOCUS       DARDHA010000211         3054 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000211 DARDHA010000000
VERSION     DARDHA010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.215"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..354)
                     /locus_tag="SDA10_002678"
                     /pseudo
     CDS             complement(1..354)
                     /locus_tag="SDA10_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(614..1162)
                     /locus_tag="SDA10_002679"
     CDS             complement(614..1162)
                     /locus_tag="SDA10_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241543.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1163..2020)
                     /locus_tag="SDA10_002680"
     CDS             complement(1163..2020)
                     /locus_tag="SDA10_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG4241544.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2306..2592)
                     /locus_tag="SDA10_002681"
                     /pseudo
     CDS             complement(2306..2592)
                     /locus_tag="SDA10_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2582..2911)
                     /locus_tag="SDA10_002682"
     CDS             complement(2582..2911)
                     /locus_tag="SDA10_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG4241545.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          893 a          620 c          384 g         1157 t
ORIGIN      
        1 taaatgttct tactgcctct tccacactaa tttcttcgtt caatttcact aataatactt
       61 gatctcgctt attataatcg ctttcattta tcctaagagt caaatctttt tccaccataa
      121 cttttacacc caataaattt tgcggtttat ttttctctgt tgaaattctt tgtgtagcct
      181 ttaaactgac aattagctca ttgggtacat acttatttga gagtcatcta attggctcat
      241 aatcctttct ttttggttag tatctgcagt tacttgttta gtatgagcca tgaacccaaa
      301 catactcacc ccaacaacca atcctaaata tattttcttt ttaaactttt tcatctacat
      361 ctcctcctaa tagttttatt ttagagttag ctctattact tatttactaa ctcaacaaca
      421 gattatcata cacttgttaa tgaaatcaca atatctcatt aaaacacacc ctaaaatcat
      481 aaccgttcct atgctcattt tttggatttt ttaatcacgt aaccagaaaa ataaatcccc
      541 tctatttcac gcaaatcaaa gaagaactaa gtaactaccg tttaaaggta gttacttagt
      601 tcttctttga ttattatcta actttattat tcattcccca ccctttgcgc agtgagtctt
      661 cggtatcttg ttttatctga ctggtagttc ttattatttc tccacgatca tctaattctg
      721 gaacaattgg atatttttct ctgaacaagt ctagttcttt caccacgtca ttaaacaaat
      781 cttgcttatc ttcttgcgta agattctgtt taatatactc atctaaagct tttctaagca
      841 attcatctct tgtcatatta gtctcatact tttctactaa aactctggaa gtcgcattta
      901 acaaagtcga cagttttaaa ggatgcttgg tggaataaac cttatcaaac tttctgattg
      961 gtgcgccaac tttttttgtt cttcctctac tctgtctttc aacattggag gaagttttta
     1021 tttcttgaac ttggtgcact tctaattgct tattttcaga tggtgtaatt gcttgctgct
     1081 ttttctttgc ttctgcttct ttttttattt tggcgagtcc tttgttgttc ccaaaaatat
     1141 caagtccttt gatgtcagcc aattactcca cctccgcata ccaattctcg tgttcttcca
     1201 attctttcaa aatactgata aataattcgt ggcttttgcg atcccattgt tttaagagac
     1261 ctttctttgt cttctccatc gtaatacctg atccgtcgta tcgttttaag cgcgcatttt
     1321 tgagtacaac atttttaatc aaatgttccc cataaatctc ttgggcttgt ttatacattt
     1381 cctgatctac tgcgtcaccg ctttccaaca tgaaaggaat aacaccagca acatataaat
     1441 ttgtatcaaa ttctttcaca aaaaagtcca tatactcaag atattgcatc gcatttctca
     1501 tcgattttac ttgggtctgt aagacgacga ttgcccaatc cgcaatgtac atcgcggatt
     1561 tactatatac agaaatagaa ggtgggacat ctaaatagat gacgtcaaac tcatcaaata
     1621 atggttctag cttttctttc atatatttga attgggataa tgaatcatta tattggtctt
     1681 tgatccaatc atcatacatt tcaaaggaca tgtctgctgg aagtatatag aggttattga
     1741 ttactttata gacagcctct ttaggtggaa gattgtttat caacacgtca gtcacagtat
     1801 tttcgtaaac aatcggttca ccttctccgc ctccttcttc gttagtgaag ttcatatgcg
     1861 ttaagttcat cacatcagaa gtatttcctt gcatatccaa gtcaattatc aaggttcttt
     1921 ttccttcatt aactgctgat tcaaacccta acatttgcgc gcacgtactt ttacctacgc
     1981 cacctttaaa attaccgatt acatatttct tcgctaccac gttcctacct cttttctttt
     2041 agcaatattc atttgatact attataacat atttattttt agttactacc aaaaaaaaga
     2101 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
     2161 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
     2221 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa aagaagccgt
     2281 ccttcttaag aacgacaaca aatcactaga ttaatttttt caactgttca taaaagtttt
     2341 catgcgttcc acataacaat actggaataa ccttgttttc tataatctcg taagctactc
     2401 tatatgttgt gcccgcatat ttgaacccac tagaccaaat acctgataaa tccccagttt
     2461 tttgttcacc cttatatgga tcaatcgcaa tttcatcata aattaaattc aaaaattttt
     2521 ctttcaatgg cttctctttt agctttttaa atacttaatg acatgttttg aaggaacgat
     2581 ttcataagcc aatttctgtt gccaactcct ctttagtcat tactttagaa ttagtaagtg
     2641 tatcttcagc aatatctcta aaggaagaat gcattttgtt ttttctattc ttaaattcta
     2701 caagaatctc atctttatcg tagccttctg caattatatc agacaaaata tcttcgctaa
     2761 aatcataaaa ttctttttgt ggttcaacaa attcataaag aataccattc tctaccaact
     2821 ttgcttcaac ttcgactcca ttaggaacat taaaatcttt aggtactgtc aacatgattg
     2881 aattgccttg ttttcttgtt tttgtaatca tttaaatccc tcatttctaa cagtatgtaa
     2941 ttacttagta aatacattat accacgagat aatgtattta caagtaatta ctgtgactcg
     3001 taatgcgtcc ttgctgaata aatttcaacc acgtgttcat cttcatctac ctta
//
LOCUS       DARDHA010000212         3041 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000212 DARDHA010000000
VERSION     DARDHA010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.216"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(425..670)
                     /locus_tag="SDA10_002683"
     CDS             complement(425..670)
                     /locus_tag="SDA10_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4241546.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(664..1065)
                     /locus_tag="SDA10_002684"
     CDS             complement(664..1065)
                     /locus_tag="SDA10_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG4241547.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(1237..3030)
                     /gene="uidA"
                     /locus_tag="SDA10_002685"
     CDS             complement(1237..3030)
                     /gene="uidA"
                     /locus_tag="SDA10_002685"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEG4241548.1"
                     /translation="MTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESYPISVP
                     SSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNGKLINQ
                     HKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKKENLVN
                     FDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILIAIYDE
                     EAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGIRSIQV
                     SNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTSHYPYS
                     EEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKMAIKEL
                     IERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYLKSTPD
                     ICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTEYGADT
                     VAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILRVQGNK
                     KGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
BASE COUNT          933 a          575 c          403 g         1130 t
ORIGIN      
        1 aacgttcaat agaaaaacta aatttacatg atgctatttg cctcactgaa tttggtctta
       61 aaaataatag aaaaaatgtt gaaatcaaca tatggaaata attcttaatc agctgctttt
      121 gtcaaaagca cataggaatt tcacctctct ccaagtttac ggcgagcctt acggaagtat
      181 cattatccca acttcaaatc atcgcttttt attgactata aaaattattt tagaatgttt
      241 atcgctcctt ctttacagta agaaatcatg gcgcaactaa aatatgctcc ttgaagtgtt
      301 aacgtactga tagaatgtat ttaaatttca atgatcaata ggttttctgt ttaaaagaat
      361 ctactaatcc tttaactatt agaagattct tttaaacaga ataagtggaa aggggacttt
      421 ttagttagtt aattttaaat tttaagatcg cttctatcaa tttaatttct agctgtctta
      481 gcatatactc atctacatag gtaataaatt gccccgcctc attaaatcca tttgtcaaac
      541 ataacttagc tatataacta tgataatgtt tgagtatcat cttgattgaa attggatctt
      601 cttctagagc agattggata atggatagag gaactaatgg atactgccgt tttttggggt
      661 tgtctaccat aagtcatacc ccatttttaa ttgttcaact gccttctttc gttgatacca
      721 gataccactt gtactcatat tgaggatttt accgatttca gtatcattaa aacctgcaaa
      781 ataataaagt aggataacct ttcttcttat ttccgataaa tgatcaatgg ctgccgataa
      841 cttttgatct gaaattaata tttccattcc aaataagaaa tagacatctt gttcatcaaa
      901 atgagtttgg gagtacttac cttcatttga attcgtcaat ggttcctcat tatctgttat
      961 gttaatgttt cgttttcgaa tagacttagt ttcgtttcgt aatacttttt tacagtaggc
     1021 atcaaattga tgttgtatag atttctcatt gtaattttgt tgcatgataa atatgctcct
     1081 tttttcccct ttccactaat acaacaatca gaaagcgcct ttttttcgaa aaaactagaa
     1141 aaaaatatcc ttctttaaac agaaaatctt tttataaaaa aaaggagttg gcaattaagc
     1201 agccactcct tttttatatt ccttataaaa ggtaaattat tttttataac tgaaatttgg
     1261 tattgatgac catcgtttct ttagatagtg agcaatcatt ttaggctttc tatctctagt
     1321 aaagattcct ttcttatttc cttgcactct taaaatccct tgacttgttt caaaatctgc
     1381 aaaattccac acttgttcac caacaaaatt gtcacattta tctattactt cgtgatttgc
     1441 ttcgtaataa cccacttgat actcttctgt aaacattgtt ggtactgtat catgaagtcc
     1501 agctactgta tctgctccat attctgtaaa cattattggc gtatcgggac atctttccat
     1561 ccatccatct agctcttttt tcaacagata ttttgcatgt tttaggtctc cacctgaaac
     1621 ataccaacca taatagcgat ttaaacataa aacatcgaca atatctccta ctttacaaat
     1681 atctggtgtt gactttaagt aagtaacgat tgttacaggt ctcttctgag ggtcaagttc
     1741 tttagtcaat tcaactaaag gttcaaaata ttctttagcg ccttcagaat ccgaatctgg
     1801 ttcatttgca atagaccaca ttacgacact cggatggttc ttgtcgcgtt ctatcaattc
     1861 cttaatcgcc attttgtgag cattttctgt ccgtatttct ttccaagtat catgtttttc
     1921 tacgttgtca tctaataaag tagccatgaa attcaaatgt aatcctactg caggtacttc
     1981 atcaataact acaatacctt ctcgatctgc taagcgcata atttcttctg aataaggata
     2041 atgtgaagtc ctaaatgaat tagcaccgat ccatttcatt aaattaaaat ctttgatatt
     2101 tactgcttca ttcaaaccac gaccattaat atacgtgtcc tcatgcttcc caaatccctt
     2161 aaagtaaaaa ggtttattat taatcaaaaa cttcccattc gaaacctgaa tactacgaat
     2221 gccaaattct tctgtgtacg tatcaatcac tttattttcg ttaattaaac taactttcat
     2281 actatataag taagcattta gaggctccca aagtgttggg ttcttaagtt gaatcactcc
     2341 ttctttcccc ttagcttctg ctataagttg agcttcttcg tcatagattg ctattagaat
     2401 ttctccggtc cattctgtat cgatattaaa ttctacattt gcatctgaat caattatttt
     2461 tgtattaata gtgacatcct ttatatagtc tttcggagtt gtatacaatt taacgggtct
     2521 attaatgcct gcataattga aaaagtcaaa attaaccaaa ttttcttttt ttatgtctcc
     2581 atcttcctgt atcgtttctt tcaaaatccc tacaggtaaa gtgctatcgt caataacatt
     2641 gtttacagcc actgtaattc tgttacttcc atcttttata aactgattaa tttctgattc
     2701 aaatggcgta aaccctcctt tgtgttgatt aatcaatttt ccattcaaaa atacttttgc
     2761 ttcatgtgtt gcagatccaa aacgaagaac taatctttct tctaataatt ttttaggaag
     2821 aatcacttct ttttcatacc acacccatcc aacatgattt ctaatgttct ctgtttcaaa
     2881 taaatcatta taagaagaag gaactgaaat tggataactc tctttttcaa ccattggttc
     2941 taaagcaagt tcttcttgta agtcatcatt tgtatctaac ttaaatctcc aaagaccgct
     3001 taaatcaatt acttgcctcg tctctgtcat aatgggatat a
//
LOCUS       DARDHA010000213         3013 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000213 DARDHA010000000
VERSION     DARDHA010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.217"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            177..359
                     /locus_tag="SDA10_002686"
     CDS             177..359
                     /locus_tag="SDA10_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEG4241549.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="SDA10_002687"
     CDS             462..746
                     /locus_tag="SDA10_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241550.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="SDA10_002688"
     CDS             762..998
                     /locus_tag="SDA10_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241551.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="SDA10_002689"
     CDS             1020..1274
                     /locus_tag="SDA10_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241552.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="SDA10_002690"
     CDS             1356..1541
                     /locus_tag="SDA10_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEG4241553.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="SDA10_002691"
     CDS             1608..2003
                     /locus_tag="SDA10_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEG4241554.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="SDA10_002692"
     CDS             1993..2370
                     /locus_tag="SDA10_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241555.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2392..2766
                     /locus_tag="SDA10_002693"
     CDS             2392..2766
                     /locus_tag="SDA10_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241556.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          925 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc ttt
//
LOCUS       DARDHA010000214         2984 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000214 DARDHA010000000
VERSION     DARDHA010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.218"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            202..1077
                     /locus_tag="SDA10_002694"
     CDS             202..1077
                     /locus_tag="SDA10_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241557.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="SDA10_002695"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="SDA10_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="SDA10_002696"
     CDS             complement(1642..2973)
                     /locus_tag="SDA10_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG4241558.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT         1054 a          541 c          463 g          926 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcc
//
LOCUS       DARDHA010000215         2922 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000215 DARDHA010000000
VERSION     DARDHA010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.219"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            353..775
                     /locus_tag="SDA10_002697"
     CDS             353..775
                     /locus_tag="SDA10_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG4241559.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            772..1002
                     /locus_tag="SDA10_002698"
     CDS             772..1002
                     /locus_tag="SDA10_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4241560.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            1500..1700
                     /locus_tag="SDA10_002699"
     CDS             1500..1700
                     /locus_tag="SDA10_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG4241561.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            1727..2920
                     /locus_tag="SDA10_002700"
     CDS             1727..2920
                     /locus_tag="SDA10_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4241562.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
BASE COUNT         1035 a          477 c          545 g          865 t
ORIGIN      
        1 catactccta gaaagtagat aaaattggcg acttaatgga gggaaaagtc gccatgagaa
       61 agataatctg tcatctagtt ctcttaatta agttcttatc aaaaaaactt atccacctgt
      121 actggataga tatgaccatc aatgtggttt tgcagattac atagatagaa aaatcaaagc
      181 atatgaacag agaaaacaat ctgtttatgt gcttttttgg tcacttaata aatatctttt
      241 aggatttctc ttcatttatg caacaaatcc cccgttcgtg tcggggttta gtatgaaggg
      301 agataaagaa gcacatgcca ctccctttca cgacagaaag ggggtgaaaa gcatgaaacc
      361 atcttctttt caagaaacca tagaaaatca gtttgactat atctgcaaac aagttattga
      421 agatgaacga aaagattact tcaaacatct agacagacta tcagcaaaag aagtctcgtt
      481 ttctgatata gataactacc ttgtcaactc tttttctact gttgatacgt acgtcactga
      541 tttccaacta tttaaattat ttggattaag aatcaatgtt gaaagtgatt tattaagtga
      601 agccctgcgt catctttcag agaaaaaacg gaacattatt ttacttcatt actttatgga
      661 tatgagtgat gtggagattg cggagttact aaatttgaat cgttcgactg tttatcgtca
      721 tcgaataagt ggactagcca tgattaaaga attcatgaag gagtgtaaag aatgaaaaca
      781 gcttatccaa gggttccctt ccccctcatt gtaaaggcga cagatggcga cgtggaagca
      841 attaatcaga ttgttaaaca ttatcgaggg tatacctcaa aacgttcatt acgtcgcatg
      901 acagacgaat atggaaattc tcatatggtt attgatgaaa ccctacgtgg cagaatggaa
      961 acgaaactca tctctaagat tttatcgttc gaaattaaat agtcccattt tttcgtggaa
     1021 gcgtgtcgaa tcgtatccac gcttcccgat ttgggaggtt tgttattcca tcaaagcata
     1081 ttgtgtgttc agtaggtttt gataggctaa caagccaaac gttctttgac aaccaaataa
     1141 aggcaatcga atacgtttcg gtaagaaaag agctgatgaa ctaacacgcc atgacctaca
     1201 aaattagtga gcgattcatg ttagtaattc gaaagtaatc tttagcagga ttgctgccat
     1261 gactttctta tcgtgataat gatactcccg tatagccata gtccgagcgt tagaagcgtc
     1321 acaggcaatg ggtacggcta catgagaacc atgcagaagt gaaattcctg tgagctttgc
     1381 tagagctgtt cgattgctaa gatattcttc tcttgtcagt gtattattat aaaactattt
     1441 gatataatta taggtgaata ttggagtttt cctagcctat aattgggaaa ggagtaataa
     1501 tgataacggt cacaaaaaag gatttagttg agctaggata tggaacttct ttttcagcag
     1561 acatcataaa aaaagcaaaa gaattgatga ttacaaaagg gcatatctac tatcaatctc
     1621 gaaagctaga tagagtccct cgtgaagctg ttgaagaaat tctaggaatt cggttttcaa
     1681 atgaaaacag gtctgagtag tttttgcaca gtaaggagta aaaactatgg ttaaagaccc
     1741 aataaaaaaa gcaaagaatg gaacctatta ttttcgtgca aatctaggtt atgattctaa
     1801 agggaaaaaa attcaaaaat atcgtagtgg attcactact aaaaaagaag caagagaaga
     1861 atattcgaaa ctgctattga tgaaaccaga agaattaagt gagaataagg ataaaatgac
     1921 gtttaaacat tatatctttg aaattttcct tccatggtat aagacacagg taaagctcag
     1981 aacatatgaa aatcgcttac caactattaa gaagcatttc tcttattttg ataaaatggc
     2041 tgtttccgat attgagccta ttgatgtgca aaattggcaa ctaaaattat caaagaagtg
     2101 taagtcttct tatgtaagag ctgttcaagg actattttca gttgctatgg atcgagcaat
     2161 tgtccttggg ataaccacca caaatccatc taagattatt ggcaatgtga aaaagcaaaa
     2221 gtctaaaatt gaattttgga caaaagaaga atttgaaaaa gtaatctctc taatttacaa
     2281 agaagattac tatcaacact ttttatttat ctctctgtgg tttttattta tgactggaat
     2341 gcgaattggt gaagctactg ccattcaatg ggaagatatt gattttgact caggagtact
     2401 aaccatcaac aaaacactct attacaagaa tcaatcgaat tatagatttg ttgaaccaaa
     2461 aacaaaggcg agcgttcgtc atattgtatt agataaatgc acattaactt atttatccga
     2521 atggaaggaa gtacaacaaa atctaattca gacagacttt gtaatgagct ataacggtat
     2581 tcctacccaa aaacatacac tatcatatgc tattgaacgc tatgctaagc tggctggtat
     2641 ccatcgaatc agaatccatg ggttacggca ttctcatgct tccttgttaa tcagtatggg
     2701 agaaaatcca cttatcatta aagatcgtct aggacacgaa gatattgaga cgactttagg
     2761 aacgtatgga catctttatc ctaacagtaa tttcgaggta gctaacaaat taaatggagc
     2821 catttccttt aaagaagcaa aacaaaatta tgatacttca ccaaagaatc aatttactgt
     2881 agactacttg cgaaacaaag aaatgaaaaa agtgcaataa aa
//
LOCUS       DARDHA010000216         2892 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000216 DARDHA010000000
VERSION     DARDHA010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.220"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..113
                     /locus_tag="SDA10_002701"
     CDS             <1..113
                     /locus_tag="SDA10_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG4241563.1"
                     /translation="SVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(199..1293)
                     /locus_tag="SDA10_002702"
     CDS             complement(199..1293)
                     /locus_tag="SDA10_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEG4241564.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1482..2243
                     /locus_tag="SDA10_002703"
     CDS             1482..2243
                     /locus_tag="SDA10_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG4241565.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2358..2798)
                     /gene="fabZ"
                     /locus_tag="SDA10_002704"
     CDS             complement(2358..2798)
                     /gene="fabZ"
                     /locus_tag="SDA10_002704"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEG4241566.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          925 a          574 c          481 g          912 t
ORIGIN      
        1 ccagtgtcag caatcaacgt aatcatattc caagaaaatc attgaattac agaacaccaa
       61 tcgaaatatt tttgagctat gtacaagaag cattttattc tagcttaatt tgacaaatca
      121 taacctataa aaaaacaaaa taaaaaagca gaaataactg gctatttcta gcggattatt
      181 tctgcaataa ctaattatct attttttatt ttcaattaat ttaaagcttg accatggttt
      241 caaaaagtca aggaggaata attcgtcatc ggagattctt cctacaacat tgacacgtcc
      301 gtcgtttttc atatccttca aggcgatttg tgtttctcct ttgtattgac cgtacccttc
      361 attgtcaatc agtacatccc catgatgcat atctcgcgta ttatgaggcg ggaaatcttt
      421 atctttgtag tatacacgag tcatcgttga acgaaggata tattctgagc gatcccctcg
      481 ataactatga aggttatcaa acaagcagat tcgttcgttt tctgtaatac cttcttccgt
      541 atctactttc aatgttggat aatccgcgtt aaatgcttct gccatttctt tcaattcttc
      601 ttcacttgcg tacgcatttc caatagaaat atcatcaatc aatcctgtaa ggattaaatg
      661 cttcacttgt gtcgcaattt caagatctcg gtggtcttcc agcgtgcaca agccatcttg
      721 cgtaggccaa gggccaaact cagcagattg cgaattgacg aatgccatcg tattcaaatt
      781 atattttctg aatttttctg aacaaaaaac aaagtgttcg tatcctaaac cagagtatcg
      841 atgaggataa aaattatgag aacctaataa attatccgtg tttggcgaat aactcatgat
      901 gttgtcaacg taattcgtgc ccgaactcat attgatctcg attttgatac caaaaggatt
      961 gcgcgtcatt tttgcttctt ctgctcctgt gaatccgata tctaagcgga ccccgtaggc
     1021 acccatctta tggaaaaaag ataagtcatc gtaagaaatc tccaactgtt caaataacgc
     1081 tggattgata tcaaccatta cttccatgcc taggctattc gcgtaatcaa ctacttcttt
     1141 aaattctgaa agaacttttt ctttatcatc attgatctgc agcaaactgg taaaaacccg
     1201 tttataccca tacttatgag ctaaatccaa gtacgctttg tccttttcaa atgttgaccg
     1261 ttctggataa atcgagattc ctaattttcc catattacca tttcctcctg catcttttcg
     1321 tttttgtatc cacttacatt atagcaaaca caaagtaaat ggtctacacc ttttcttttt
     1381 cttttaaaat aaatgataaa ttccaataat ttaagtaggt attttccctc tattttgtta
     1441 tcattaaaga taacaaaata gtaaattgga aaggaacagt tatgaagaaa aataaagtta
     1501 ttttaagtat tagtttagca gcagcattgc tgtttgctgc tggttgttct gcaaacaaca
     1561 cgacttcttc tagttcgtct acagaaagtt caaaaagtac atctactgcg gaaagcacga
     1621 ctgccagtag cgtcgatttg aacgcattgg aattaccaca attgacgaaa gatgtcgccg
     1681 aaaatgaaga tctggtcgaa atggtgacga ataaaggcac aatcaaaatc aaattatttc
     1741 ccgaacacgc accaaaagcg gttgaaaact ttatgacaca tgcaaaagac ggttactaca
     1801 acggactgac tttccatcgc gtcatcaaag atttcatgat ccaaggtggc gatcctaaag
     1861 gcgatggcac tggaggggaa agcatttggg gagaaggatt tgaaacagag atcaacaacc
     1921 acctttataa tataagaggt gcgttatcta tggctcgctc acaagatcct aacagtaacg
     1981 gcagccagtt ctttatcgtt caaaatacag atgatatgca tgatggtctt ttaaaagacg
     2041 actatcctca agcaatcatc gacgcttata aaaacggcgg ttatccttca ctagatggcc
     2101 attatactgt cttcggccaa gtgattgaag gaatggatgt cgtggatgct attgcaaata
     2161 tcgaaacaga tagttcagac aaaccaaaag aagaagtaaa aatcgataaa atcaatatac
     2221 tgcaagaagc aaaacaaaaa taaaaaaata acgtctgaat cctgcataga tgacaacgta
     2281 tgctaggatt caggcgtctt ctttttacat aaaaagcaag taagcttcct tttgttttgc
     2341 tatatcaggt tttatagtca agcattttgg ttgacgatga acgtgaactc gcactcacag
     2401 acttttttat catctacata tgctgcagat aacgctgtac cgactgatcc cttcattttg
     2461 gtgatttcaa aatgacattt cagcacatct cctggaacaa ctttttgacg gaatttggcc
     2521 ttattgatcc caccgatata agctcttttt cctagaaact cttctgattt taggataagt
     2581 atcgaacctg cttgtgccag tgtttctaga atcaacgcac ccggcatcac tggattgtca
     2641 ggaaagtgac cttggaaaaa atcttcattg attgttacat tttttgtcgc aacgatcttt
     2701 tttcctggtt ctaaagtatc tacataatca atataacaaa taggataacg attaggaatc
     2761 aattccatta cttcagtagc agataatacg taagacataa ttaaactcct tctttcaggc
     2821 aattgccatt ttcattactt tgactatcaa agtatcatat tcctaaatat ttagcaatct
     2881 ttttttaatt ta
//
LOCUS       DARDHA010000217         2775 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000217 DARDHA010000000
VERSION     DARDHA010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.222"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            449..>2775
                     /gene="esp"
                     /locus_tag="SDA10_002705"
     CDS             449..>2775
                     /gene="esp"
                     /locus_tag="SDA10_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HEG4241567.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1005 a          426 c          548 g          796 t
ORIGIN      
        1 gaaatgctgt gaaaccgata ctttataatt ataaagaagg tgatttcaaa ttccgaagaa
       61 ggaatttgaa gtcacctttt tacttatgtc acagtctcat tcagtaatgc gggataaggc
      121 tccttattcc gttatttgaa tactctgaag tttaatctaa gaaagggtaa tattttgaaa
      181 acatgcttca tcgcatagaa atgaacttaa tttcagaaag aatacataca tatcttttta
      241 tatgttttca gctgctgaat ggttaaaaag cacaaatatc cgaaaaaaac atgaagaaat
      301 gtactttttt tgttgtaatg ttcatgaaat tctctaagta aattagttaa cataaggtta
      361 gtaagattcc tattctgtga ataatacgta tcagatttat cttttattct tactaggaaa
      421 gtaattatta agggagttga tttgaataat ggttagcaag aataataaga gagtatttct
      481 agaaaaaaca aagaaacgag tattgaagta cagtatcaaa aaattaagtg ttggggttgc
      541 atctgttttg attggtgttg gtcttgtctt gggaacaact gaactagtta aagcacaaga
      601 tgaaatttct ccaagcagcc cacttgaaac agcgataagc tccgtacaag taggtgacaa
      661 agtagcttcg gggaatactt ttcaggagaa tcctggttat acgaaaaatt ataacttttc
      721 ggatttacaa tttagtcctc aagaactaac tggagacaca ctaaaaggca atacaattgg
      781 atttgaagtt tacggaaaac ataatattgc agcatcaact aaaaactggg aaatccgtct
      841 tcaattagat gaacgattag ctaaatatgt agaaaaaatt caggtcgatc caaagaaagg
      901 aattggatct tctagacgga cctttgtaag aattaatgat tcgctgggta gacctacaaa
      961 tatttggaag gtcaattata ttcgtgcaag tgatggatta tttgctgggg cagaaacaac
     1021 cgatacgcaa accgctccta atggggtaat tacgtttgaa aagagcctag atgaaatttt
     1081 taaagagata ggcatagaca atctcaagac cgatcgttta atgtatcgaa tctacttggt
     1141 aagtcatcaa gacgatgata aaattgtacc gggtattgat agtacgggct actttttaac
     1201 agattcagat gatttctata atagcttaga tgtatctgaa aataatcctg accagtttaa
     1261 acatggatca gtaagtgcaa aatatgagaa acctaatact caaacaaaag atggttcagg
     1321 atccacaggt gctaatggag ctataatctt agatcataag ttaacgaaaa attataattt
     1381 ctcatattca tcttccgcaa agggcacgcc ttggtatgct aattacaaaa ttgatgaacg
     1441 attagttcca tatgtagcgg gtatacagat gcacatggtt caggctgaca aagtaacata
     1501 tgatgtttcg tttgaatccg gaacaaaagt agctgattta gcaatcgagc gacgggaagg
     1561 tcatgaaaat tatggcatag gttcaataac taataatgat ttaactaaac ttatcgattt
     1621 tgctaatgca agtccacgtc cagtcgttat tcgatatgtt ttacaattga ctaagccatt
     1681 agatgaaatc ttagaagata tgaaagcaac agcacaagtt gaagaaaata aaccatttgg
     1741 tgaagatttc atctttgatt cttggttgtc ggatacgaat aaaaaattaa tccagaacac
     1801 ttatggaaca ggttattatt atttgcaaga tattgatggt gatggaaacc ctgacgataa
     1861 agaagagagc ggagacacga atccatatat cgggaaacct gaattagaag aagtatatga
     1921 tgttgacaca acagttaagg ggaaagtatt catccacgag ttagcgggaa caggtcacaa
     1981 agcccaactt gttgataaag aaggtactgt attagcagaa aaaactatcg ctccaaatga
     2041 aaaagatggg gctccaattt cagatactgt agaatttgaa tttacgggtg tagattcaag
     2101 taaattaatc gcgaaagatg aattaaaaat ccaaatcgtt tctccaggtt ttgataaacc
     2161 agaagaaggt tcaaccgtta ttaaggaatc accaaaagcg gttgataaac aaaccgtggt
     2221 agttggattt aaaccagatg ctaaagaatc aattcggaat aataaaaact tacctgaaga
     2281 tgcagagtat tcatggaaaa cagagcctga tacttctaac gttactgata gtacgaaagg
     2341 tattgtaact gttaagatcg gaaatcgaac tttcgacgtg gatgtagagt ttgctgtaaa
     2401 agcttctcaa gctatggaaa atgatgcaac atacgtacct ataacaacaa ctccagaaac
     2461 gacagttcaa agtggaaaac ctacatttga taaaccagat gttcctttag ctaaagatgc
     2521 cttttcaatt tcagatgtct ataataagta cttcggtaat gcaagtgttg atgcaaacac
     2581 tggtattgtt acattcactc cagctaaagg tgtaggagaa tcggagccga ttactggaac
     2641 aattcctatt aaaattgttt accaagatgg ttctgtaggc acgaccgatt tagcagtaac
     2701 tgtaagtaaa gatatttatg aaaatccagg agaaaacatt cctgcaggct accacaaagt
     2761 aaccttcacc gcagg
//
LOCUS       DARDHA010000218         2752 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000218 DARDHA010000000
VERSION     DARDHA010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.223"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..443)
                     /locus_tag="SDA10_002706"
     CDS             complement(<1..443)
                     /locus_tag="SDA10_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG4241568.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRRFKTGEFNVLHLPMQGKRKPNGYKEKRGKQAFKRNIS"
     gene            513..863
                     /locus_tag="SDA10_002707"
     CDS             513..863
                     /locus_tag="SDA10_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4241569.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            1277..2095
                     /locus_tag="SDA10_002708"
     CDS             1277..2095
                     /locus_tag="SDA10_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG4241570.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(2168..2527)
                     /locus_tag="SDA10_002709"
     CDS             complement(2168..2527)
                     /locus_tag="SDA10_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG4241571.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          878 a          421 c          517 g          936 t
ORIGIN      
        1 tcagaaatat ttcttttgaa tgcctgtttt ccacgttttt ccttataacc atttggtttt
       61 cgttttcctt gcatcggtaa atgtaaaaca ttgaattctc ccgttttaaa ccggcgatag
      121 agggtactta ctgaacaaga gaaggttcgc tctgcacggc caataatgac gtctggggtc
      181 caaccttcag ttgatctttt tgcaatgtat tctttttcct cagcaggaaa aatgatttcg
      241 gttctcccac aacgtcgctt attttctttg tattgttcaa agtattcaag tgctgttcca
      301 ccacatttga gaaagttata gacattgtaa attgtttggc gtccacgttt aagctgcttc
      361 gcaacgatgg caaccggagt ttcttgatga aaatatgctt ctatcattac aagctcgttt
      421 ggtgtaagat gggtataagt catttatgtt cactctcctt gtatgcttta gcgggtatta
      481 caatttgagt gtaacataaa tggctttttt atttgtctcg cttaattata caaacggcgt
      541 tttataaagc aatcattcct gagaacttag atgaggttaa actttattta tgggaagata
      601 acggagaaat tgtcggtttt agtggtgtaa gtgcagaaga attagttatg ctatttcttg
      661 atccagattt tattggtaag cgatatggta gtcaaatatt aattgagctg attgaaactg
      721 aagatataaa aagaattgat gttaatagtc aaaataaata tgcaaaacat ttttatttaa
      781 gccatggatt tgaaattgaa tctgaagaag acttagatgg ttttggtaaa ccatacccta
      841 taacgcattt agtaaaaaaa taattcatga atggtttatt acctgattga acatgaataa
      901 aagtaatatt tttattttga tggcctattt ctaggttatg aattatccag atcaaatttg
      961 tgtattacat tcagtaataa ggtgcttatc ttaggatcaa cttatgatac tagctgacca
     1021 aggatgtacc agttatagaa aatcagttaa agtaaaaatg ataaaaatct tatgtcaaca
     1081 ctgatttagg ctgacctgtg ggttaggaga aagacaggtc ggtagctttt aatacaaaaa
     1141 atggcttgta tgtatctaga tagttaactg aaattagaaa tgttcttatt tttaacggag
     1201 taagaaaaat aggctgcttt aggtctgaat actttttcaa tataatgaag atatcaaagg
     1261 cgacaggagt gtttttatga ataagaatcc tcataatcta aagttggtag cagtagatat
     1321 ggatggaaca tttgctcgta ctgattacac atacgatatc ccaaggttca aggctgtttt
     1381 atcgcgaatg aaagctgttg attgtcaatt tgttgttgca agtggaaatc aatactacca
     1441 attgagatcg ttatttccaa attattatga tgaattaagt tttgttgctg aaaatggtgc
     1501 ttttgttaaa gataaaacag aggtactatt ttcagccgat atgcctagaa aagcgatcga
     1561 ggtggtcctt gatttatgta aagactatcc tgaagtaaaa aatgtgatgt gtggtctgaa
     1621 tagtgcctac tgtgaaagag gaaaggtgag tcaagcattt tttgatctaa ccaatatcta
     1681 ttaccataga ttagaatggg tagctgattt tgcagaagtt caagataaaa ttttaaaatt
     1741 tgctccgact atgcctgttg aaaaaaccga attttacgtt gaattgttcc gtgaaaaatt
     1801 agccgggcta ttagtaccta ccagtagtgg tcatggttct gtagacttga ttctttctga
     1861 gtgccataaa actttcggct tgaaaatgtt agttgagcgc ttggggatta atcctgacca
     1921 gtgcgttgct tttggagatg ggggcaatga cattgagatg ttggagtatt gtggtctgag
     1981 ttatgaaatg gataatgcaa cggaagctgt taagcaagtt gctaagcatc aatgtccttc
     2041 aaatgacgaa gacggtgttt tagtgacgct cgatcgattg ttccctaaca cgtaaacact
     2101 aggtaattca tcaaaaaacc tcgatataag aaatattcga ggttttaaaa ctatatttag
     2161 aacagattta cgaagcgtct gatgagtcaa aatagacttg gacattgcta tctaaaatac
     2221 cactttcatt ctttaaacga attgtttgat ttgatggagt tacctctaat actaaatgcc
     2281 cattaataaa ggtagtatcg tattcagatg ttttaactgt tgcttcttcg caaggtattg
     2341 ttaaatcata tgctggaata taatgttgtg tggaaactaa taaaaaatga tcaccaatca
     2401 tacagcatcc ctctgctgaa gaaaattgat tagagccatc atttaaagtt aacaagaatc
     2461 catcctttaa acgaggtact tgcttaaata cttctggttg tacttctaaa aataaattat
     2521 cgctcatttt cacgaccacc atcgacttcc acagttgaaa ttgacatttt atttaacaca
     2581 cctattcccc cttaactata actcaattct atataaaaaa tattctattc tcaaataatt
     2641 gaacttttca aatcgccaat ttacttcggc tttatcagag ctcattcata tcaagagcga
     2701 ttaacgctgt aaaaattaaa ccgaagatct gataaatttt ctctattttc ta
//
LOCUS       DARDHA010000219         2739 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000219 DARDHA010000000
VERSION     DARDHA010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.224"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="SDA10_002710"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="SDA10_002710"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEG4241572.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1072 a          425 c          558 g          684 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatata
//
LOCUS       DARDHA010000220         2688 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000220 DARDHA010000000
VERSION     DARDHA010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.225"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..445)
                     /locus_tag="SDA10_002711"
     CDS             complement(<1..445)
                     /locus_tag="SDA10_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241573.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPI"
     gene            532..1230
                     /locus_tag="SDA10_002712"
     CDS             532..1230
                     /locus_tag="SDA10_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEG4241574.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            1408..1749
                     /locus_tag="SDA10_002713"
     CDS             1408..1749
                     /locus_tag="SDA10_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241575.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            1742..2413
                     /locus_tag="SDA10_002714"
     CDS             1742..2413
                     /locus_tag="SDA10_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEG4241576.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            2419..>2688
                     /locus_tag="SDA10_002715"
     CDS             2419..>2688
                     /locus_tag="SDA10_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241577.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
BASE COUNT          977 a          404 c          541 g          766 t
ORIGIN      
        1 ctattgggaa gtttgtattt ttgatatcaa caattttgtg gatttgggtt ctatttagag
       61 taaatatcat ttttgtttgg tgattaagga gtttattaaa attaattttc cctacttcaa
      121 atgcaaggta acaagatata gcacttttag gaggaatatt tagcggtaca gcttcagagt
      181 atattctatt atcatcgact gatcctcctt tagcaattaa tttcttaaat ggagtagctg
      241 accatctgcg gtttaagtta gcggatgaaa gttctaaatt aactaacata gctggttctg
      301 ttgaatagtt agaaatgatt acttttgtat aaatcatatc tgcagcgaag taagaagcat
      361 taagttcaac ttctagttga gggcgcattc cttttatttt tatccctgtt aaaaacgtac
      421 tcaatataaa acttattatt gacatccatt gaaaaattgt taagtgaaaa aaattcaaaa
      481 taaacaccac cagtttttaa ctaaattata ccaaaaagga gagaagaaat aatgcaagaa
      541 ttagtaattt tgaaaaataa agaagctgtg actacgagct tacaagtggc agaaagcttc
      601 gagaaaaaac atcaacatgt tttaagagat attgatgcac taaaaaaaga tgtgtccaat
      661 tttggacaga tgtttgtaga aggtaatgaa ccagattcat atggcagaaa tcgacgagtt
      721 ttcttcatta gtagagatgg atttttcttg ctggctatgg ggtttacagg aaagaaagct
      781 atctacttca aacaaaaata cattgaagca ttcaacgaaa tggaagatgt tattcgcaag
      841 aatactgttc ctcaaacaat tgaagatatg atgatctatc aattagaaga aatgaaagat
      901 gttaaaaaag atgtttccat gcttaaagat actatgcgaa ttagcggaca acaagagttt
      961 gaaattaagc aaaaaggaaa tatgaaagtt atgggagttc tagggggaaa agaaagccga
     1021 gcttatgaag aaatcagcaa aaaagtattc tcaaaatttt ggtctgaatt taaacgtacc
     1081 ttttcaatcc caagatatgg cgagttacct cgtaagagat tcgatgatgc tgtttcattt
     1141 attgaaatgt ggttgccaga aactgcgatc cgtatggaaa ttgatcaact gaacagacaa
     1201 cagagacttt ttggtgatga caatgaatag agctgaagcg ctaagaatag ggacggcaat
     1261 tgctaattgc tggtggaaat actataaacc aatcatccta agccaacaac atattgacaa
     1321 gcaaaaagca tggcaacaaa taaaaaagtg actccgccgg caagcaaaga gtcacaaaga
     1381 aaacacatca taaggagatt ttagcatatg gaaaaagaac tttccactct agatcaatat
     1441 ttgattgatc ctgattgggg caagccgaaa attgaggaaa caagtggtcg aaaaatcaga
     1501 cgaaatcttt tgacgaatga agaactagct tgggatcaag atgatttagg caaccatgta
     1561 actatttggg atcatgttta tcttatccat ctatcgaagc attcgaataa acctgaatat
     1621 atttacgtca tcgaagatgg cttgattgat gcgctagaag agtacgacag agataacttg
     1681 attgatatct cttattacgg accaggtaag aaatacattg ctgaaatgga ggcagaattt
     1741 gatgagtgaa atcaaaggga caacgaactt tgaaaaactt tttagtcgta agttaaataa
     1801 aattctcaag aaaaaaggaa attttgatta tttatcttgg gctcacgcgt gggagattat
     1861 gaaaaagaat gatccacagg caacggtaac tattaatgag tacaaacact acagggttgt
     1921 ttctggaact catcaagact ttcttgttga ggaatataaa ccttttctta tggatgaaac
     1981 tgggacttat gtatctgtct cagtaacggt taaaggacac acggaaaccg aattatttcc
     2041 tgttttagat tatcgaaacc aaccagttgt taaaccaaat gcaatgcaaa tcaataactc
     2101 attgaagcga tgctttgtga aagcattggc tctacacgga ctgggattat atgtatttca
     2161 aggggaagat attccaacac cacctagaat cgatacaaag aaattaaaca tgctagagac
     2221 gattctagaa gctttcaatg agcagatggg taaagatatg accaaaacct taatcgaata
     2281 tgttaatgag cagacagata aattagggct cttagctgat aacgttgaaa ctattgaaca
     2341 gttaagctat gagcaatgtg ccttgatgga gcgagcaata gcagctaaga gaaaagaatt
     2401 agataagaag tgatatgagt gtttaaacca ttaatcgatt catattcagc ggttctgaaa
     2461 aagttcaaag gaaaagacat aagcgcaacc atcaatgagg aagtgaacat tgatcgacta
     2521 aagacgatgt atgacggcta cgatggtgat cgagtcattg aaattcgttt tattgatcct
     2581 agacgtttca ccgtacagca acgaaacttc atctatgcgc tgataggcga tatttttatc
     2641 gatacaggca tgccaacgga cttctggaag gaattcttct acttccgt
//
LOCUS       DARDHA010000221         2678 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000221 DARDHA010000000
VERSION     DARDHA010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.226"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            137..1144
                     /locus_tag="SDA10_002716"
     CDS             137..1144
                     /locus_tag="SDA10_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG4241578.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1156..2160
                     /gene="neuB"
                     /locus_tag="SDA10_002717"
     CDS             1156..2160
                     /gene="neuB"
                     /locus_tag="SDA10_002717"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEG4241579.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2157..>2678
                     /locus_tag="SDA10_002718"
     CDS             2157..>2678
                     /locus_tag="SDA10_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEG4241580.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAP"
BASE COUNT          975 a          342 c          529 g          832 t
ORIGIN      
        1 gatacttcat attttaatca atcaaatgtt gattcgttac gttttgacat cattattgat
       61 aattagttta gttatatatt ttttaaagat taagaatcga attatagatg actataattc
      121 ataggaggag atataagtgt ttaaaggaaa aacaatatta gtaactggcg gaactggttc
      181 cattggaagt gaaattgttc gtcagctttt aaaatatgaa ccaaaagcta tcagaattta
      241 tagtagaggt gaagaaaaac aattttatat gcaacaagag ctctcaaatc acactaattt
      301 gagattttta attggtgata tcagagactc tcaaaggatt gattatgcat gtcgtgatgt
      361 ggatattatt tttcatgctg ccgcaatgaa acacgttcca gcaagtgaat ataatcctat
      421 ggaagctgta aaaacaaatg tgatgggaac tcaaaatgta atagatgcag cgatcaaaaa
      481 taatgtaaaa cattttgtag gtatcagtac agataaagta gtgagtccta ctaacaccat
      541 gggtgcgaca aagttattga gtgaaaaatt aataatagct gccaatcagt ataaaggaaa
      601 aacagaaaca atattttctt gtgtaaggtt tggaaatgtc atggggtcaa gtggatctgt
      661 tattcctctt tttagtaaac aaataaaaaa caataaagat attactttga cagatgttga
      721 tatgacacga tttatgatgt caataccgga tgctgcaaga ttagttctgt ctgctgctat
      781 gaattcaaaa tttggagaaa catatgtctt aaaaatgccg gcaatcaata ttttaacttt
      841 agctcaatca ttaataaaaa tatataatga tacacatgat gaaaaatata gaggtgaaat
      901 aaaagttgta ggtatcaggc caggagaaaa attatatgaa gagctaatga ctacagacga
      961 aattgaacgc tcatatgaaa atgataaaat gtatatcatt ccaccacact atcatgctac
     1021 tgacgaactg tacaaagatt atgaaaaaat taaaatcact gcatactctt cagatgcaac
     1081 agaattaatg agtggacagg aatttattag aatcgcaaaa gagaataatg tcgtgacttg
     1141 gtaaggagtt ttatgatgaa atcagaagta tatgtaattg cagaaattgg tgtaaaccat
     1201 aatggaagtc tagatttagc tttagaaagt attgataaag ctaaagaatg tggagctaac
     1261 gcggttaaat ttcaaacatt taaaacgaat aagctagttt ccaaaaaggc tccaatggca
     1321 aaatatcaga aggataatct taaagaggac agttctcaat ttgaaatgct aaaaaaatta
     1381 gaattatctc aatctgattt tattagaata aaagattatt gtatacaaaa taaaattgat
     1441 tttttgtcta ctccttttga tgaagaaagt gcagactttc taaaacaaat tggtgtacaa
     1501 ggatttaaaa ttggctcagg agatttaaca aatttacctt ttcttagaaa aatagatatg
     1561 tatgggttgc cagttttatt atctacaggt atggctgata ttcatgaggt cagtgaggct
     1621 gtagattgtt tcatagatag tccagttact attttacatt gtacttcaaa ttatccggct
     1681 agttctgaag atattaattt attggcgatt aacacattaa aagaaatgtt cgggataccc
     1741 gttgggtact ccgatcattc attgggatat gatgttggta tttgtgctgt tgcgatgggt
     1801 gctaaggtaa ttgagaaaca ttttaccttg gataaggaac tacctggtcc agatcataaa
     1861 gcttctttag atcctcagga atttgcagat tttgtaaagc atattagaaa cactgaaata
     1921 tttttaggtg atggtgttaa gcgagcaatg ccttctgaac ttagtacaaa agaagtatca
     1981 agaaaaagcc tagtggtcac tgagaattta aatgtgggag atattcttac agagaaatct
     2041 cttacaataa aacgtcctgg aaatggaata gctcctaagt ttttatatca atatattggc
     2101 aaaaaagtta aaagaccaat aagtgcagat agtgttctgc ttgaggaaga tgttgaatga
     2161 aaaaaatact tatagtaagt ggaacacgag ctgattatgg aatatataaa cctgttgctg
     2221 agaatttact acaagataat tgggatgtat catttgttgt ttctggaatg catttatccc
     2281 ataaatatgg atttactaaa gaattgatct tagctgacgg atttagaatt agtggagaag
     2341 ttcaaagttt gtttttagag aacaataagg gaaacatggc tcggtctgtg gcattagaga
     2401 taaatggttt cacaaatata tttgaacaaa ctagccctga ttttgtatta ttactagggg
     2461 atagaggaga aatgttggca gctgcgatag cttgtttgta tcttggaatc aaaacagcac
     2521 atattcacgg aggcgaagtg tctggtactg tagatgaatc aataagacat gccataacga
     2581 aaatagcatc tgttcatttt gctgctacgg aaaaaagcaa agagcgttta ataaaaatgg
     2641 gggaagattc ttggagagtt tttcccgtcg gagctcct
//
LOCUS       DARDHA010000222         2659 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000222 DARDHA010000000
VERSION     DARDHA010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.227"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1256)
                     /locus_tag="SDA10_002719"
     CDS             complement(<1..1256)
                     /locus_tag="SDA10_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG4241581.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
     gene            complement(1270..2487)
                     /locus_tag="SDA10_002720"
     CDS             complement(1270..2487)
                     /locus_tag="SDA10_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG4241582.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(2477..>2659)
                     /locus_tag="SDA10_002721"
     CDS             complement(2477..>2659)
                     /locus_tag="SDA10_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEG4241583.1"
                     /translation="DTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPE
                     NEAWLQGALEVFKHEQ"
BASE COUNT          725 a          576 c          439 g          919 t
ORIGIN      
        1 actacaaaca cgatatgttg ccccattaat ttttggaagc gattggttcc ttttttctct
       61 tcctgtttct ttgcatattt caacgccgca tctagttttt gacgggttgt aatgatcaca
      121 acattcgcat tggcatctgc caacagagtt tcacttaact ctttggcact tcctgttccc
      181 acgatcaagc tattcgcttt ggcattacca gaagaaaccc cagtattaaa ctccgaagca
      241 aatttcgtaa actctgtggt ggtctgatta tctaaatctt tgcggtccac taacatgatc
      301 gttcgatcga tccccgattt tcgtgctaaa agcttagtag atacaaaact agtcaacgtc
      361 tttcctgaac cagtcgcatg ccacacatac cctgattgat gtttatttgc ggcaataaac
      421 aaggcttgaa tcgcatgcac ttgataagga tgtaacacca tgagggtttt attgtcttga
      481 tcttcactga caatcgtata atccgcaatc aagcgatggg catctgggat atttagcact
      541 tgtttgcaaa actcatataa gttttctacc tttttattat ctttcgttcg ccaactaaat
      601 aaaaacttag gatgcaaatc cttcggtaag gcatttgcaa aataccgagt cgtttgttca
      661 ttggaaacca caaacaactg taaggtcgaa aagatattgt tccgaaacat gccttcttca
      721 gcatattttt tgatttgatt aaacgcctga taaaaaccat cttttgcagt tacttgtttt
      781 agctcgattt ggataatcgg taagccattg atcaataagg tgacgtcaaa acgtcgatcc
      841 cgcgcttcta tgttacttcc acgttttgca atttgatgga ccacttcata agtagagatt
      901 cctccaccaa tgtcttgatt ggaataaagg ataagtgaga cagaacccaa ttgcggattt
      961 tcccgttcaa tcgtgatccg agccatccca ttttcacctt ttaaccattt cgcagcatca
     1021 aatggggttt tcgtccgtag caataattcg gttttgatcg tttcaaactc tttatcggtc
     1081 aaaggagaat cattcagttc tgcttgatta tttgagatga ttttttgacg taaattgctc
     1141 caaagatctt cttctgattt tagatccgga cgatacgtcc actgattatg tccttccccg
     1201 aggacacgaa tcaactgttc ttcgacttcc gcttcctcac ggtgactaat tttcattctt
     1261 cttccctccc taaacaaaca tcttttgcaa gaagcctttt ttcgtttcct tcagcaaatc
     1321 taacttacgt tgatgaagag cgatcgtatc atctagctgc ttgaagaagg caccaatttt
     1381 ttgttgttca tttatatccg gtggaaactg aaatgtcctt tgttctatac cacctttagt
     1441 cacatgtacc attgtcgttc cgttagttga ttctttaatt ttctctttat cagtcaatag
     1501 ccaagtatat agatactgtt tgtccaattc cttagataaa aatttaagtt tccaaatatg
     1561 ataatggtag attacacgtt cttcattcca catttcagga ccgaagcttg ttgcccataa
     1621 atataagaga tctcctttat tagcatactt gtcttcctct aattctaagt ttgaataata
     1681 ccatctatca ttagtattaa aattacctac tcttaaaacc ctatattttc cagaatcaag
     1741 taattcttgt tgtttaaaag ctctaccatt caaaaaaact accacttccc ccaacttccg
     1801 ctcttcccaa tctcccgtaa atcctggaaa acgaacttct ggcacttttg ctccgttttt
     1861 agggaacatt ttttgtaaga atcctttttt cgtttccttc agcaaatcta attcgtgctg
     1921 gtgaagcatg atggtgtgat caagttgttt gaaaaatgaa ccgatcttga tctgttcgtc
     1981 ataagttgga agtgagatag gtgtcttttt accactatca attgtatagg ttccgacggt
     2041 tccaatgcct gcaacgtcac ggatatactt ctgaattttg ttatttatga acgagtagta
     2101 aaagaagttt ccgtcaattt tatgctcatt cttgaaccaa atgatgtttc catccttaaa
     2161 gtaaattggg ttatcatcag tgattaataa ggggacacct attgatccga caccagttac
     2221 aagcaagtct ccttgagaaa ctttacctga atttttcgaa tattcattat atttttcttc
     2281 tgaaatgtaa agataatcgg atggttcctc attttttgca gctgagacaa tatcacgagc
     2341 acgtaaaaat ctaatacctg agttagtcca atctgattga tgaattcgct tcacagaagt
     2401 aacatccatc atatctccta acttacgctc ttcccaatct tccgtaaaac caggaaaccg
     2461 gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct tgtaaccacg
     2521 cttcattttc aggtgttgtt tgcagggaag agatagcttc taaaagttct ttttctagca
     2581 cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata tcaattggtt
     2641 cttcttcttc gaaagtatc
//
LOCUS       DARDHA010000223         2658 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000223 DARDHA010000000
VERSION     DARDHA010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.228"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..183
                     /locus_tag="SDA10_002722"
     CDS             <1..183
                     /locus_tag="SDA10_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEG4241584.1"
                     /translation="DTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAISSLQTTPE
                     NEAWLQGALEVFKHEQ"
     gene            173..1390
                     /locus_tag="SDA10_002723"
     CDS             173..1390
                     /locus_tag="SDA10_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG4241585.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            1403..>2658
                     /locus_tag="SDA10_002724"
     CDS             1403..>2658
                     /locus_tag="SDA10_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG4241586.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT          931 a          444 c          552 g          731 t
ORIGIN      
        1 gatactttcg aagaagaaga accaattgat atggttcatg tcggaaatga catcaagaag
       61 atacgacaag aacagcaagt gctagaaaaa aaacttttag aagctatctc ttccctgcaa
      121 acaacacctg aaaatgaagc gtggttacaa ggtgcgctgg aggtgttcaa acatgagcaa
      181 tgatacacaa ccagaaatcc gtttcccagg atttacagat gattgggaac agaggaagtt
      241 gggggacttc gttgtagatt atgttgaaaa gacaagtgta caaaatcaat ttcctatgct
      301 aacttcatct caacaaaaag ggatagtttt gcaagaagac tattttgcaa atcggcaagt
      361 aactacagaa aataatattg gttactttgt actacctaga ggatatttca cgttccgtag
      421 tcgaagtgat aatgatgtgt ttgtgtttaa ccgtaatgac attattgata gagggattat
      481 aagctatttt tatcctgtat ttactttaaa atctgctgat tccgacttct tcttaagacg
      541 tattaacaat ggaatccaac gacaattatc tattcaagca gagggaacag gtcaacatgt
      601 tctgtctcta aaaaagttta aaaatatagt agcaatgttt cctagtgagg aagaacaaca
      661 aaaaatcggc accttcttca aacaacttga cgacacgatc gctcttcatc agcgtaagtt
      721 agatttgctg aaggaaacga aaaaaggatt cttacaaaaa atgttccctg aaaacggagc
      781 aaaagtgcca gaaatccggt ttcctggttt tacggaagat tgggaagagc gtaagttggg
      841 agaattaggt aaaactcaat cgggaattgg atttcctgat gccgagcaag gtggttctga
      901 aggtactcca ttttttaaag tttccgacat gaataacatt ggtaatgagt atgaaatgag
      961 aaatgcaaat cattatgtat ctaatgagca aattgagcgt aaaaaatgga agccaataaa
     1021 agatgtgcca gcgattatat ttgctaaagt tggcgcagca ataatgctta atagaaaacg
     1081 tttggtcaca agccctttct taatagataa caataccatg gcttatttat ttaataatac
     1141 ttgggatatc tattttggta aaatattgtt tgaaactata aatttgccaa gatactccca
     1201 agtaggggca ttgccttctt ataacagttc tgatattgag aatatcagtg ttaaggttcc
     1261 tgtaaaggat gaacaacaaa aaatcggtac tttcttcaaa cagctagatg atacgatcgc
     1321 tcttcatcaa cgtaagttag atttactaaa agaaacgaaa aaaagcttct tacaaaagat
     1381 gtttgtctag gagggaagaa gaatgaaaat tagtcaccgt gaggaagcag aagtcgaaga
     1441 acagttgatt cgtgtacttg gagaagggca taatcagtgg acgtatcgtc cggatctaaa
     1501 atcagaagaa gacctttggg tcaatttacg ccaaaaaatt atctcaaata atcaagcaga
     1561 actgaatgat tttcctttga ccgataaaga atttgaaacg atcaaaacgg aattactgct
     1621 gcgaacgaaa accccatttg atgctgcgaa atggttaaaa ggtgaaaatg ggatggctcg
     1681 gatcacgatt gaacgggaag attcgcaatt gggttctgtc tcacttatcc tttattccaa
     1741 tcaagacatt ggtggaggaa tctctactta tgaagtggtc catcaaattg caaaacgtgg
     1801 aagtaacata gaagcacggg atcgacgttt tgacgtcacc ttattaatca atggattacc
     1861 gattgtccaa atcgagctga aacaagtaac tgcaaaagat ggtgtttatc aggcatttaa
     1921 ccaaatcaaa aaatatgcag aagaaggtat gtttcggaac aatatctttt cgaccttaca
     1981 gttgtttgtg gtttccaatg aacaaaccac tcggtatttt gcaaatgcct taccgaaaga
     2041 cttgcaccct aagtttttat ttagttggcg aacgaaagac aatgaaaaag tagaaaactt
     2101 atatgagttt tgtaaacaag tgttaaatat cccggatgct catcgtttga ttgcggatta
     2161 tacaatcgtc agtgaagatc aagacaataa aaccctcatg gtgttacatc cttatcaagt
     2221 acatgcgatt caagccttgt ttattgccgc aaataaacat caatcagggt atgtgtggca
     2281 tgcgactggt tcaggaaaga cgttgactag ttttgtatct actaagcttt tagcacgaaa
     2341 atcggggatc gatcgaacga tcatgttagt ggaccgcaaa gatttagata atcaaaccac
     2401 cacagagttt acaaaatttg cttcggaatt taatactggg atttcttccg ggaacgccaa
     2461 agcgaatagc ttgatcgttg gaacgggaag tgccaaagaa ttaagtgaaa ccctgttggc
     2521 agatgccaat gcaaatgttg taatcattac aacccgtcaa aaactagatg cggcgttgaa
     2581 atatgcaaag aaacaagaag agaaaaaagg aaccaatcgc ttccaaaaat taatgggcca
     2641 acatatcgtg tttgtagt
//
LOCUS       DARDHA010000224         2632 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000224 DARDHA010000000
VERSION     DARDHA010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.229"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            251..526
                     /locus_tag="SDA10_002725"
     CDS             251..526
                     /locus_tag="SDA10_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241587.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            539..2611
                     /locus_tag="SDA10_002726"
     CDS             539..2611
                     /locus_tag="SDA10_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEG4241588.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
BASE COUNT          805 a          413 c          567 g          847 t
ORIGIN      
        1 tcgcagcccc atttttttgt tgtgataaaa gaacaagaaa ggcaggaatg ggaaagtaaa
       61 catccttttg tcagaaactt ttatctttct taagttttac ttgatatctg atactatttg
      121 gaatagaatg aggcgttgaa aaatttatgg aaatttctaa ctagcaataa acgatgaaac
      181 aatgaaatat ttttcatcca aaaagaactt tgctataatg atggaagttt gtgtatagga
      241 gatctggaaa atgagagaga gttttgaaca acaaaaaaaa ctgttgcatg atcgctatgg
      301 cgccctttcg atggatgacc gccgtcaaat actttgtaag ttgcgtaaaa gaaatatatt
      361 gatgtatcgt cagttggaac gtctgaaaca tgatctgctt cgtttggagt caaaacgtgt
      421 acagtgtgaa ttagagggga accaaacaca ggttgaagta gtcgaaacca aaattttaaa
      481 gaaaaaagaa caatttttaa aaatgcttac acaaaataaa aaataaaggg gatggacgat
      541 ggaatttgct gagttagtta ttgtatttgc agtaacgatc accttatcga acatagctag
      601 ccgcattttg ccaatgattc cagctccact gatccaaatt ttccttggag tggtattagg
      661 tctgacagag tggggacaat caatcgattt tgaacctgaa ttgttcttag ttatgattat
      721 tgcaccattg ttatttagag aaggagaaaa agcagatatt tcttcgattc tcaaaaattt
      781 cgggacaatt ttgttcttgg catttggagg agtcattttg acccttgtcg gggtaggtgc
      841 gacgttatca ttcctgttgc caagtgtccc acttgctgcc tgctttgctt ttggagcagc
      901 gttaggtccg acagatgcgg tagcagtcag ttctctttct ggacgagtga atatccctaa
      961 aaaggcgatg catattttgg aaggagaagg tcttttgaat gatgcatctg gtgttactgc
     1021 cttccagttt gctcttggtg cgctgatcac cggatctttt tcagcagtaa acgcggggat
     1081 gagtctagta gtttctagta ttgggggagc tttgatcggc tttttgcttg tctggttcaa
     1141 acaaaaaata attcatttga tcgaaaaagc atcagcacaa gatgtcacag ggtatctgct
     1201 gatcgaactt ttgcttcctt ttctagccta tgtattggca gaggcatttg atgtatctgg
     1261 aatcattgct gcagtggcag caggtattct ccaagcttct ggttttagga aaatctctgt
     1321 gtttgatgca gaattgtcga gcttgtccca cagtacatgg acaactattg cttttacact
     1381 gaatgcatta gtatttatat ttttaggaat cgaattgacg caagtgttct cgccagtatg
     1441 gggagacggt ttatatccaa acggtctttt attagcgatt atcgtattaa tctctgtcat
     1501 gctttttgtg atcagattca tttctatcag tcttttttat gtattcaaag atggcagtaa
     1561 aaagttcaaa aaacagttga acgagatctt gatcttaaca tttggcggtg taaagggaac
     1621 agtcagtttg gctacgattt ttattttgcc attttcaatc aataatatga tgttttacca
     1681 gcggtctttg cttttgtttc tgacggcggg tgtcatctta gtgactttgg tcatagggat
     1741 cattgtcttg cctatgctga cggaaacaga ggaagcgaaa agcacggatt tgaatgcttt
     1801 gatgatttta gaagaggtcg ttgaagtttt gcgaaaagaa ataaaggaaa ttgatcgtaa
     1861 tacaaaagaa tttttagcca ctgaagcagt gatagaaaat tatcaagaac ggatacgtga
     1921 tctgtacttg gaagatttaa ccgatgatga aaagcaagaa gtacaagaaa tacaagcact
     1981 gattttgtcg atcgaacggg atggattgga tgaaagttat cgttcgggca agctttcttc
     2041 taatggttat cgtttttact cccgttttct ttctcgattc gaacattcta tcacaagcca
     2101 gattttgtct tttatcggct tttggttcat tgttgtccgt aggttaatgc gaatcgttct
     2161 ccatccgaaa atgttttggc aaagaagata tgctgatcgt cagacattta ttagtgatga
     2221 agatatccaa gaaatcagag agacatatca aaaaaatact cagttgatta ttgaaagttt
     2281 agataacttg actgatattt atgatgaaac gatggttcaa ttttttatcc agcaacgcaa
     2341 acttgaagga ataaaaatga actcggggaa tttgatttct tcgtggatga tccagcaaga
     2401 tagtctgttt acgaaaaaaa tgctaagagg ttattatctt gaaagaaaaa tcattgatga
     2461 atacgaagtt gctgaaaaaa tcacgacgtt ttctgcgaac agctatcgca ggaatatcaa
     2521 tttgttagaa tcttatacga tgaacaaacc atctgataac ttttctttcc gatttgcgtt
     2581 tcgagcaaag tcaaaaaaac gagtccaata aaataaaaag gtaaaaattt tc
//
LOCUS       DARDHA010000225         2564 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000225 DARDHA010000000
VERSION     DARDHA010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.230"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            104..>2564
                     /locus_tag="SDA10_002727"
     CDS             104..>2564
                     /locus_tag="SDA10_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG4241589.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTL"
BASE COUNT          842 a          411 c          541 g          770 t
ORIGIN      
        1 aaaaaacata aaaatcatat tagataaaaa ctaaattggt agataaatta ttttgtcaga
       61 tatctgaggc aaatattcga ctatttgtgt ttcaaagtaa aaattggaat acaaatatag
      121 aatagacata ttgcagccga tagtttatta tgcttttaga cacattaaac tggaggaaaa
      181 aatgaaaaag agcgctaaag atcgagtttt tgatgagctg atacgactaa cagggaatat
      241 gtcttctgtc actgccgaga aaatttcgaa gaatctaggt atctctcgtc agaatgccag
      301 ccattatttg acgagattag tggaagataa aaaggtcgag aaattaccgg gaaagcctgt
      361 tttgtggaag cctttagatg aatatgcggt tgtagataat acggagcaaa taaacgaagc
      421 ctttcattct gtagttggtc atgacggaag tctgagagag gtcattcaaa aatgtatagc
      481 cgctgtcaaa tatccgccta acggactcag tgtattaata aatggagcca cgggtgttgg
      541 aaaaagtttt ttagccacaa aaatttttga atacgctatc catgagcaaa ttattgaaaa
      601 agatgcacca tttgctatct taaactgtgc cgattatgcg gacaatcccg aactgctttc
      661 ggcaacactg tttggataca agaagggggc ctttacgggg gctgaaaagg ataccgatgg
      721 tttattggca acggccaaca acggctatct ttttctcgat gaggtccatc gcctatcgaa
      781 agaaaatcaa gaaaagctct ttctatttat tgatactgga aactatcgtc cagtcggtga
      841 aaatgtaaat tggcattctg ctaaggttcg ttttattttc gccacgacgg aaaaaggtga
      901 aaattattta ttagacacct ttgatcggcg tattcaaatt agtgttatgt tgccaacttt
      961 tgaagatcgc cccattcgag aacgattaga gctgattcaa ttattttttc aaaatgaagc
     1021 ggatgtttta caaaaagata ttattgtttc aagagaagcc ttgtctttaa tgttatctca
     1081 tccctttagt gggaatattg gtaagctgaa gaatatcatt aaaatcagtt gtgcggatgt
     1141 gtatagtcga acacaagaag aaccattaac gattgggaaa aatgagatct tgattcagtt
     1201 aaatatgctg gaatccgaag tagaaagctt gccgattgct agtttattga taaagaaaaa
     1261 tcaaaagata aaaacggatc gaatcaaacc gtttgattat gattcgattt tggaaaaaat
     1321 tactcagctg ataaatgaag gtaatttcaa tgaaaatttt tcttcgataa agaatgaagt
     1381 acagcatttg gtgcatcaca ttgataagga ttctttttta tcaatgagta gaaaattgat
     1441 gattcagatg tttagaaagg tctgggtatc cgttttagga aaaacgtacg gattgacgac
     1501 gagtggacct gttgcagaaa ttattagcag atcctatgtc atttccccaa gctataagac
     1561 agacttaacc gggataaatc gtacattagc tgcaaatcta cctcggacga cttacttatg
     1621 taatcagttc ttggagcatt taccaccgct ctcagaaaag gacgagaact ttctaagact
     1681 gcttattgtc gtgactttat ctgattatgt agatgaatcc atcgagctga agggcctgtt
     1741 gcttgctcac ggagatagta cggctagcag cattcaagca gtagtgaatc aattatgcgg
     1801 caattatgtt tttgaagcgt tggatatgcc agtagagagt aatgtgtcag agattgtcga
     1861 atcgacaagg aatttcgtga aacggcaagt gaatacagat agtctagtgc tgatcgtaga
     1921 tatgggctct ttaaatcaaa tttacacaca gatcaagaac gatttaaaag gggagttact
     1981 tcttctgaat aatttgacga cttccattgc acttgatatt ggcttgaaga tgacgatgaa
     2041 tgcgccattt aaacagattg cggaggatgc agcagacaag tattcaatca atgttcaata
     2101 ctttgaaggc ttttctcaaa gtagcaacat tatcatttcc tgtatgtcgg gtttggggat
     2161 ttcggacaaa ttgaaagatg tttttcgtca tcatttatcg gatgaatcca tcgaagtttt
     2221 cacgaaggac tacagagaac tacgtgaatt aatcgaccaa aatgatgaaa cttattttga
     2281 caagacaaaa ttggtcatca cgacttcgga tcttccacgt tccttttcaa ttcctaatat
     2341 taatgtttat gacatcttag acggtgatgg agcagccaat ttggagcata ttttgagtcc
     2401 tgaaatgaag ccggcagaat ttaaccgctt gattcaggag ctagtaaaat tcttttcgtt
     2461 ggaaggaatt gcggaccgtt tgaactttct gaacccaacg atcgtgatga gtgaagtgga
     2521 aacagtcatt tctaaatatg aaaattacta tcatttcact ttaa
//
LOCUS       DARDHA010000226         2554 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000226 DARDHA010000000
VERSION     DARDHA010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.231"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            75..470
                     /locus_tag="SDA10_002728"
                     /pseudo
     CDS             75..470
                     /locus_tag="SDA10_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(465..683)
                     /locus_tag="SDA10_002729"
     CDS             complement(465..683)
                     /locus_tag="SDA10_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241590.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            790..1104
                     /locus_tag="SDA10_002730"
     CDS             790..1104
                     /locus_tag="SDA10_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4241591.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="SDA10_002731"
     CDS             1208..2386
                     /locus_tag="SDA10_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4241592.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
BASE COUNT          838 a          374 c          523 g          819 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaag
//
LOCUS       DARDHA010000227         2543 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000227 DARDHA010000000
VERSION     DARDHA010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.232"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..238
                     /locus_tag="SDA10_002732"
     CDS             <1..238
                     /locus_tag="SDA10_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4241593.1"
                     /translation="FFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYT
                     EIADILGFHSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            379..1992
                     /locus_tag="SDA10_002733"
     CDS             379..1992
                     /locus_tag="SDA10_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG4241594.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT          817 a          442 c          479 g          805 t
ORIGIN      
        1 tttttttatc agtccagcca gcttaaaaaa acattttaga aaagtgaatg gaaaaagtgt
       61 tattagctat tttaaagaac taaaaatgga aaaagcaaaa cgtctcgtgc tagaaaatga
      121 attaacctat acagaaattg cagatatatt aggatttcat tcgatccatc atttttcttc
      181 agcttttaaa aaatatacag ggttatcccc ttcaaagtat tatctatcct taaaatagaa
      241 aaaaattttt aggttttatc cacaaattat ttctctatat tttctgtcta ttcaaaataa
      301 acaataattt tccggcacaa ttatttgctg ttttttacca taacatagta cgatcaacgt
      361 agaaaaggag atgtttttat gaaagttgtt gtcattggcg gtgtagctgg cggtccttca
      421 tttgccactc gtttccgtcg attgaatgaa gcgcacgaaa tcattatcta tgaacgcgga
      481 gaaaatattt cttatgcaag ttgtgctttg ccttattatt taggaggtgt gatcactgac
      541 cgtgactcgc tgatcgaacg tacaccagaa atattgaaaa caaaaaacaa catcgatgtc
      601 tttactaaac acgaagtaac agcaatcgat ccttctacta agcgattaac agttaaagac
      661 ctatccacaa atgaagaaat aaaaacagat tacgataagt tgatcatctc ttctggtgct
      721 agaccagatt atccggatat tcccggagtt tttgaagcag aaaacggctt tgtcctccgt
      781 agtgtgacgg atgcggatcg aatcaaatcg ttccttgaag aaaaaaatcc acaacatgtc
      841 gtcattcttg gtgcaggtgt tatgggtctg gaattagctg agaatttcaa gcatcgcggc
      901 ttagacgtga ctttaatcga tcaattgcca caagtcgctt tcccttatga tccagaaatt
      961 gctaatttag tttatgacaa attgctgaaa gaaggattag ccgttcattt agaaacaaga
     1021 gttactgaga tccgtgataa aggtcgagaa atagtattat cagatggctc cgtcctttct
     1081 gctgatatgc taatttttgc tgttggtgtt tctccgaaga atgaagtggt gaaagcaacc
     1141 ggcatacaat tatcagatac aggtcagatc attgttgatg accagttaca aaccaatctg
     1201 ccggacatct atgcgattgg cgatattatc gaaacgacta gtgtagtgac tggtcagccg
     1261 atccaaagta tgctttccag tgcggccaat cgtcaaggac atatgctggc agatatttta
     1321 aatggtacgc ctatgcgcta tcgcggatat attggtgcag gtgtcgcaaa aatctttgat
     1381 catacagcaa gttatgcggg aatgacagaa catgaattaa aagcatcagg cataacaaat
     1441 tataaaactg tttttatcac tccctttgac catgcctatt tctatccaga agctacaaga
     1501 ttaaatctaa agctgatttt tgatgcagat agcggtcgta ttttaggtgg acaagcattt
     1561 ggagaaaaag gtgtcgataa acggatggga gaactttctg tagcaatcac cggaaacttg
     1621 acagtctttg atttgcctga tttggagttg ccttactctc caccgtattc tactacccgt
     1681 gacccgttga atatagctgg ttatgtcgca atcaatcaaa tgacgaatat cgtagaaacg
     1741 atcaaagcaa gtgatatacc cgaaaacgat ttgaaagaag cgttcttttt agatatacgt
     1801 gaacctaata aagcaccttc tggcagtatc tcggcaacaa aaaatattcc aatgaatgag
     1861 ttacgagatc gaatcaatga aatcccaaaa gataaaaaag tttacattac tttcagaaga
     1921 ggattgaata cttatacttc tgcccgaatc ttggcaggtt tgggtatcaa agcgattttg
     1981 attgaagaat aaaaaaacga ccgcggtggg tagccgcggt ctaggagtta tttttacttg
     2041 aggagtaaaa atgaaaaagt atatagggtt gttggttggt atgtatatag aatagaagat
     2101 aaatgtgaag aaattgtgca ttatttcttc acatttatta ggttatagtt taagaaagtt
     2161 taaacattgc gcaatttaat tattttagct gtttcttttc ataaaaaaac atgttgtgac
     2221 tctgagacaa atcatagtcc atagtgatgt ctctagagta actacgccta atattacgag
     2281 gcatcatatt aatctatcag cactagtacc taaactagca tccctttcga atattttttc
     2341 ttatataata aagtttgctt tcactcctct aatctcacca aatcgactgg cttgcgttgt
     2401 gtgatactag gaactgattg gtttagaacg acttttctat tgtaatcata tttttcgcca
     2461 tcctcgttgt cttctaatcc ttcaatgaat gatccctaat gtgtgtatta aataatttaa
     2521 tttttttgtt cctatttatt taa
//
LOCUS       DARDHA010000228         2506 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000228 DARDHA010000000
VERSION     DARDHA010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.233"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..352)
                     /locus_tag="SDA10_002734"
     CDS             complement(<1..352)
                     /locus_tag="SDA10_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEG4241595.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFLNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGEL"
     gene            571..1941
                     /locus_tag="SDA10_002735"
     CDS             571..1941
                     /locus_tag="SDA10_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HEG4241596.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1962..2246
                     /locus_tag="SDA10_002736"
     CDS             1962..2246
                     /locus_tag="SDA10_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241597.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          849 a          375 c          518 g          764 t
ORIGIN      
        1 ctagttcacc tttggtgagc ttcttgatga aaaagtatcg aataatcttg atagtgtaag
       61 ctaagtttga acttaagcga gtgaatcgac tcctactagg aaagtcacca ttaagaaata
      121 agacggaaca aaccgctcta tacgtggctc tttggctagt atagccatta attatgcccc
      181 atataacaca agcaataatc actgtatcgc gttgcttcag ttgatcagta tttcttcggt
      241 ttttaatctt atctggtaca caattgtggt atatgttaga aacagttctc ataatttcct
      301 tgaatgttat aaaagtatct gtataatgtt cagtatattt tgatgaacgc atatgtagtc
      361 ctcatttctt gggagttttg agactactat atgcgttttt tgttgattta ccaacatgtt
      421 tagacatttt cctctaggaa aaaattaact agcaccacgc gtattgttta tattaaatta
      481 gatgaccgtg ataatgcaca gcaaattttt gaaagtatca attctaccgg tgaaagattg
      541 actgcctcgg atttgattcg aaacttcatc atgatggata aatctaatga agagcagact
      601 acgctttata ctaaatattg gcgtaggttg gaagaagtct ttgataattc taaaggaatg
      661 gaagactttt tcagatacta ccttgctgca ataactgggg agtattcagc aaagcatatt
      721 ctttatcaag cattcaaaga ctattggcat aaagagcgcg atgtatcaag tgatgccgaa
      781 ttgctccaaa aattagtgcg atatgctggt tattttgcga gactttacta taacaaacct
      841 gaaggaaaat actcagaagt attgagcgat ttccaaagta tggaaagtat gatgcctgca
      901 ccttttgttt tgggattatc tgaatggtac tatcatgacc aatttatcac tgaagaccaa
      961 tatattgatg cgattaaggt agtgaatgat tatcagctta ggagatattt taatggggat
     1021 gatacaagca gagtgtcaaa agcatttcct tcatatttga aaagtgttag gaagtacgct
     1081 aaagctaatg gatatgaaaa tatcgttgat attgtaattt atgttcttgt tactagaaat
     1141 caatcaaata atatggcact tccatctgac aaggcattga aatctaatct attgaatgcc
     1201 aatgcttatg cgatgagatt ggcccgttgg ttacttgaaa aaatagagaa tcgagaaaat
     1261 tcagcaacac ttgatatgag taacttgtcc attgaacaca tcatgccaca gactagtact
     1321 tcatattggg aagagaaggc tggtacatcg ggggaagaat acacaggatt agtaaatacc
     1381 attggtaatt taacactcgt tacaaatcct gataattcgg ccgcgggaaa taaagacttt
     1441 gagaccaaaa agaaaatctt tgaagatacc cttcatattc gtatgaataa agatttgtac
     1501 gaattgactg agtggacatc atcggatatt tctgccagaa gtgaagctct aatcaatgaa
     1561 ctgattacta tgtatcccta tttgagatcg tcgggcgact atgaacacga tgggaatagg
     1621 gaaattttct tagaggcgca aggaataaaa gcaactgggt atttgaacga agacgagacc
     1681 gtgattatac attcaggttc agaaatttat tctaagatta aagatattgc atctgattcg
     1741 ctagatgaaa ctcgccaaga gctgttggat aatggaatca ttgaagaaac aattggggga
     1801 ctacaatttg ttcaagatta cactgcctca tctgtttcga atgcagcagc tctgctatta
     1861 gggggtagcc ggaatggttg ggactactgg aaatatgata acggaataag tattaatgat
     1921 tcgctaagaa ataagaaata gagcatagag gtggtgttag gttggataag aacaaactaa
     1981 gatatgcaat tcttaaattg cgtgatgaaa aaaagaatcc ttttattgag cttaaagggc
     2041 aagttccaga agaagatatt ttagaacaaa ctaaatttct ttcgcgcaat ggcttactag
     2101 aatccatggt ttgggcggat aacactgttc atatctgggg gagtgtttct ctggaagggg
     2161 aaaaatattt agttgaaaac tcaggtcttg ctaaggcgta tgcattggca aaagaagtta
     2221 aaaattggat tccattttgg ggctaagata agtttttaaa cggagaaggt agcaaatcat
     2281 aattggtttg ttgccttttt tgattgccca aaaagaaaaa tgacaaagta tagagataaa
     2341 tgacatagta cacgggaaaa atgacattgt atcaccgaca acgtctaaat agatgttgat
     2401 atatatacgt tccgtacaaa taattataaa aatttaaatg atttgagtag aagtatcagt
     2461 gaatatctca aggggaatag ctatctcaag attcgatttt cacata
//
LOCUS       DARDHA010000229         2450 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000229 DARDHA010000000
VERSION     DARDHA010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.234"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(18..650)
                     /locus_tag="SDA10_002737"
     CDS             complement(18..650)
                     /locus_tag="SDA10_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HEG4241598.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            complement(999..2450)
                     /locus_tag="SDA10_002738"
     CDS             complement(999..2450)
                     /locus_tag="SDA10_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEG4241599.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
BASE COUNT          697 a          484 c          361 g          908 t
ORIGIN      
        1 aaagttatat acgcaatcta tttctcttta gaacgataaa ctcccttaac accacttgta
       61 ttatttttcg gtaattttga cgttagacac tctattactg tcccttcaac acatgtcctt
      121 gtttcttgcc tttttaaaca tccacaactt tgagtatgtc caaaaaataa gttactataa
      181 cttatctcac acatactgcc acactcacaa tgacaaaccc attttgtttt ccatgaacag
      241 ttattgtcca aaggatactt tgcagtaagt aatccgaatt ttttattttc aatgtgatgc
      301 ttttttgatt tcaaacatcc acagcttttt acatcacctt ttagaagtcg ctttgccgat
      361 actaactttt ttctcccaca cagacattga cattcccaaa caacagttcc ttcacttcgc
      421 agtttagttg gcttgattgc aactaactta ccaaaagttt tatttgtcaa atttttatat
      481 ttatgcctat ttttataatt tccacatcca caacttttgg ttctacctga tagtaagttt
      541 gatcgatata caatcgtttc attcccacag tcacataggc aacgatattt tttatatcca
      601 ttatgagtaa tctcctcaac aacagtaagg tgaccatatc tttccttcac tgcacctacc
      661 ctcccaagaa agaagtacca tataatttca attaactttt aataaatcag aaaaaaatat
      721 ttccaatttt tcttagtctg attttatgac taatttacaa tacaagactg aacgatacgt
      781 acacgaaatc ctcaagatag ataaattgtt ttcatctagt gaaacctgct caaaatatga
      841 gcataaagac ggcaataata cactcgatat tcgataatgg acttgtttta tttgtcaaac
      901 ccatcgtaat cgagatatca attccagtct caatatcctg atcaaaggtc taagaatcca
      961 accattttct tgttactttg tcagattgat tacctttttt agtctccgaa taataaggtt
     1021 ttcggatcaa ctaacatgct ctgagaaaga ctactatctt tgcaaatttc caaatgaagg
     1081 tgtgggccag tactattccc agtacttcct acatagccaa ttatttgtcc ttgcaaaaca
     1141 ttatctccag tttttactag gtattgttgc tggtgagcat ataacgcggt tgatccatct
     1201 tcgtgttgta tcaccacgta atttccccaa gagtagtgaa actctgcttt aacaacctta
     1261 ccagctttaa ctgcatgaat cggctcaccc tgtgtagctg ccatatcaag tcctcggtga
     1321 aattcatttc cacgatttcc aaacaaactg gttattgaat aattatgaat aggcacttgg
     1381 tatccagtct tactcatttg tggaccttta acttctttat catagttggt taaatcgtag
     1441 gttttaatta attcatttag cttcaaatag tattgtgtat ctgttgcata ccgtcctgta
     1501 aggaatttag ttgcttctcg atatgtttta ctattagctt ttaatgctcc tgcataaaaa
     1561 tctttatttc cactaattcc cttagttaat aatttggcat aatcttcaaa gctttcctca
     1621 tagttttcat acactctaaa ttttgattgg gtcgtataaa gcgttccatt acccaaatct
     1681 tcgtttgtta ccataatgac aaagtttcca ttataagttc cttttatccc aaataaattg
     1741 taattcggtg cctgagctaa ctggctttga ccactggcag attctaagat cgcttgtgca
     1801 atcataacag aagcatataa atcatatttc tgaccaattt ttctcgcaga ctcaccaatt
     1861 ttcataataa aattttctac agattcattt ttgtcaaaat gaatttcttc attaccagat
     1921 agtattggtt gtgattggaa agattctgtt gattctattt gctgtggtgt cgaaaaagtt
     1981 tttgtttgtc cttccgtttt tggcgcttcc ttttttgaag attctatatg gtttggtttt
     2041 gtcactttcg aagaagttat ttcacttgta ctacttgaag ttgttgaaga ttcggtactt
     2101 gaaatcgtac tactttcact tgacgaagtt gttgaagact cggcatttga agtcgtacta
     2161 ctttcacttg atgaagttgt tgaagactcg gcatttgaag tcgtactact ttcacttgat
     2221 gaagttgttg aagactcggc atttgaagtc gtactacttt cacttgatga agttgtcaaa
     2281 agttcactac tctctgctgt tacatcggaa ttactcattt cagtcgtctc ggttctttct
     2341 tttgcaattt tgtatgttga attttcgctt gccaacacat ggaaaggtgt tgctataagg
     2401 agttgggaca ttaatataac tgctgatgca agttttaaag attttttcat
//
LOCUS       DARDHA010000230         2417 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000230 DARDHA010000000
VERSION     DARDHA010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.235"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            113..2266
                     /locus_tag="SDA10_002739"
     CDS             113..2266
                     /locus_tag="SDA10_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEG4241600.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          824 a          425 c          535 g          633 t
ORIGIN      
        1 attttatctt gaaattaggt aaagaatttc ttgctccgcc cattttttct tttttattaa
       61 gaagtttttg ggtacaatga agcatgaatg taaataggcg gaaggatagg aaatgatgaa
      121 agattttatg aaaaaaatat taaatacttc ttggttagaa aaaataagaa aaggtgcaca
      181 agaacctaag aacaaaacgt ttcgccggtc ccatgttcct tttcgcttaa attttctttt
      241 tttcattata tttacgttat ttgttgcgtt gattgctaga ttgggttatt tgcagatcgt
      301 gaacggcgaa gagatggagg cgcgagtgaa gtctacttct acgattacgg tccaagaaag
      361 tactcctcgc ggaatgatct acgactccac aggaaaagct ttagttacga ataaagcgaa
      421 tcaggccatc acatatacga gaggaacaca agttacggct aaagatttgc ttgaaatcgc
      481 aacgaagctg aactcgctga tcgatgttcc ggtagatgaa aatctgacag atagagataa
      541 aaaagatttt tggctggctg atcccaaaca tttgaaagaa gccacaaaaa gactttcggc
      601 aaaagaaaag caattaggga acagcgaaca atatagtgcg atggtcaata aagtcacagc
      661 tgatgagatt aatttaaacg aggagcaatt aaaaattgct accatcttta aacggatgaa
      721 tagtgcatat gaattaaaca ctgtctatat caaaaactct ggtgttactg atcaagagtt
      781 agctgttgtg gcagaacgcg cttctgaact tccaggagtt tcgactggca cagactggac
      841 acgagaatat aatgcagcaa gttcactgaa aagtattctt ggcagtgtga cgactgaaaa
      901 gcaaggactt ccagcagatg aagcagagaa atacctagca aaaggttatt ctcggaatga
      961 ccgtgtagga caaagttatt tggaaaaaca atatgaagat gtcctgcaag gaaccaaaac
     1021 ccaatatgaa gtttcccttg ataatgaagg aaatgtttcg aatcaaaaag aaatcttctc
     1081 tggggaaaaa ggttctaatt taatgttatc tatgaatgca gaattccaat caaaagtaga
     1141 agaaattttg aaaaggaatt atcaaacatt gatcaacaac ggcaaagcac agtattcgcc
     1201 tggtgcttat gcagtggcta tgaatcctca gacaggagaa gtgctagcaa tgactggttt
     1261 cagtcatgaa caaggttcca aagaaatcac cgagaatgct ttaggcacga ttactagcgc
     1321 ctttgatcca gggtctgtag tcaaggctgg tactttgaca gcaggctggg caagtaacgc
     1381 gatcagcgga aatcaagttt tgattgatga gccgattcgt ctgcaaggtg cttcagaaaa
     1441 aagctctgtc tttaatcgaa gcggacaagt tgctcttgat gcagtcaaag catcggaatt
     1501 gtcttctaat acgtatatga tcaaagtggc gttaaagatg ttaggactag actatacacc
     1561 tggaatggga ctacctagtt tagatgaaga agctaaagct tatcagcaat tgagagactc
     1621 tttcaaagag tttggtcttg gtacaacgac tgggatcgat ttgccgaatg aatctcctgg
     1681 gatctcacgt tctgttgatt atatgaagaa gttcaatgcg gataatggca aagaatggta
     1741 tgctccaggt aactttaccg atttggcatt tggacagttt gacacatata caccaatcca
     1801 attagctcaa tatgcttcaa cagtcgctaa tggcgggaaa cgagtacagc cgcacctagt
     1861 caaagcgatc tatggcaatg acgaaaatgg aaacttgggt gaagtgaaga aagaaattgg
     1921 cacaacagta gaaaatacgg tcaatatttc tgcagaaaat atggcgatcc tccgtgaagg
     1981 attccaccaa gttgtccatg gtaccgatcc ttacacaaca gccaaaccat tagccagtgc
     2041 gaaaatggac ctttccgcaa aaacagggac tgcagaaacg gttgcggaag gtcatccaga
     2101 tattgcgaca gtcaatagta atatcgtggc atacggacca acagacaatc cggaaattgc
     2161 catcagtgtt gtcttgccga acttattaga cgaacaagac cacataaact tgacgattgc
     2221 taaagaaata atggacgcgt attatgatat gttcatggca aaataaaaaa gaggtcgtga
     2281 aaaagccgct tagtttcgag tatgaagaaa aattttgtga aaatagcttc tcacattttt
     2341 gcttaaattt gacttcatac cgagaaactg gcttttgaac accgtttact cggcaaaaaa
     2401 ggttgtgaca aaaattg
//
LOCUS       DARDHA010000231         2407 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000231 DARDHA010000000
VERSION     DARDHA010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.236"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            5..1150
                     /locus_tag="SDA10_002740"
     CDS             5..1150
                     /locus_tag="SDA10_002740"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEG4241601.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="SDA10_002741"
     CDS             1321..1779
                     /locus_tag="SDA10_002741"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEG4241602.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="SDA10_002742"
     CDS             1938..>2407
                     /locus_tag="SDA10_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEG4241603.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DARDHA010000232         2401 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000232 DARDHA010000000
VERSION     DARDHA010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.237"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            208..2070
                     /locus_tag="SDA10_002743"
     CDS             208..2070
                     /locus_tag="SDA10_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG4241604.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2070..>2401
                     /locus_tag="SDA10_002744"
     CDS             2070..>2401
                     /locus_tag="SDA10_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG4241605.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          867 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaaatttg aaaaagcgtt tgattattta tattacgact tagtgcaata aaaaacgctt
       61 tttaacagta catgtagtcg tctttataat agataaactc tgtaatatta gttaaaaagc
      121 tataagttgc acttagttaa gtgcaaacat ttcatattgt cgttagttta gaaaacgctt
      181 atattagcaa tgtaaggaga gatgataatg cagcagctaa caaaaagaga aaggcagctt
      241 ttgatttact tggtggaaat gtctgaaccg gtaacaatca aaaagttggc tgaaaaagaa
      301 aatgttagtg ttcggacgat aaaatacgat ctagatgata taagagagtg gctgaatgag
      361 cggaagcaag tcctatatag caaacgtagt cagggaattt ggatgaatat tagtagctct
      421 gatcgaatca aattaaaaag cgaattgatg gatgttaatc gttttgaaat atttgcagat
      481 caaagtctcc gaatcaaacg tttactaatt atgcttttag taacaaaaga agctattact
      541 ggaatgaaaa tggccaacgc attagatgtc agcaaagata cgatcatgaa tgatttagat
      601 gtgctggaaa ataattttct taaggaagga ttgaccttga atcgtcaggc gcgaaagggt
      661 ttttggatca taggagaaga acgtctagtt cgattaacga ttgaagagat tcttcaaaaa
      721 gagttcacgg attatgacat ttataaatta atgtcattgt tattgaatgg cggtgagact
      781 gaatactttg aaatgtattc tgctactgct acacctatcc aagaggtatt taatcaagta
      841 atcattcgaa tgcgacactt actggaattt gaaaatctag agaaactgaa ttacgcggaa
      901 ttactaaata ttctgattcg agtgacaatt gctaccgttc gcttacgcaa agaggctacg
      961 attggtcgtt atcaattggt tgctgaacaa gaattagaca aaaacgatct ttcttatcgc
     1021 gtcatgcagc aggtctttga ttattatcag ttgccactgt tcgaggatga atatcattat
     1081 atctatagtg atacattcga cacgattcac cagcaagacg tcatgaaact agttgagaat
     1141 gtgatttata aagtaagtaa agcaatcaac cattcttttt atcaagatcc acaattgttg
     1201 acgaatctgt atgctcattt atccatgcga ttatcgagaa agaaaaaatt tgtcaatgaa
     1261 tataatcctt ttaaagaaga tatcaagcag aaatacccgg aattatttga tgcgatcaag
     1321 caagcagttc gacaagaaat aagtgaacaa aatattctga tcaatgattc tttcgtggca
     1381 tatattgcct tacacttttt ggtttcctac gaaagagaag aagatctacg aagcgttaga
     1441 gccgtctaca tttgttcaac agggttgggt gtgactagtc tgatcaagca aaagatcaat
     1501 gaagaattaa ataatatcac tatcgtggca tttgcttcag tattgaatgc gcaagaaatg
     1561 attgctacca aagatcctga tttggtgatt agtatttttc caattaatgg catcgatcgt
     1621 cctttcatca aagtccatcc attacccact gaacaggact tgaaaaaaat tcaacaaata
     1681 ataaaaaaaa tacttgcttc ttccaatcag caggaaaggc ataacttgaa tcttagagaa
     1741 acgtccatca ataaagatac tttagaagac tttagcaagg aattgatttt gaaagcttat
     1801 acgatttatg aacgtttact tgtcattttg gaagcagaat tgatccaaga atatcgagaa
     1861 gcttttttgc ttcatgtatt cttaatggtt catcgtatca catttgatca acaatatatg
     1921 gcagaaggtc gtcaagtaga ccagattatt ttagacaaag gaacactggt aggaaaaatc
     1981 gaagcagttt ttgcagaaaa taatttagga atcaatcagt cagaaatcag tgcattattc
     2041 tcatatataa aagaaggagc gtcaagttag tgaaaatgtc agacgaggct ttaaaaatta
     2101 tcgaggaaag tcaaaatcgg gaaaagttaa aacaagtgat tgatctaatt gaagagcgat
     2161 tagtagaaca tgctattgta ccaaccgagt tgcagtggac cattctaatt aatcacttga
     2221 atgagatgct gaaacgttct caaagaaagg aaacgattcc cgtagtcgat cgggatttgt
     2281 tcaaagaagt atccaaagaa gccataacta tttctgatga aatagttaga agtatcggta
     2341 atttaaccga tgatgaaata tatgttttgt cgattcattt tgaaacagca aaaaataatt
     2401 a
//
LOCUS       DARDHA010000233         2293 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000233 DARDHA010000000
VERSION     DARDHA010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.238"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            12..1382
                     /locus_tag="SDA10_002745"
     CDS             12..1382
                     /locus_tag="SDA10_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241606.1"
                     /translation="MSFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIA
                     LTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLS
                     KQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISD
                     SIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWF
                     LFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSI
                     AAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVG
                     ESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNV
                     DVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEIN
                     KKNENK"
     gene            1369..>2293
                     /locus_tag="SDA10_002746"
     CDS             1369..>2293
                     /locus_tag="SDA10_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241607.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKI"
BASE COUNT          768 a          342 c          392 g          791 t
ORIGIN      
        1 gtacgattct gattagtttt tattatggag aaaagttttt taaaaagata gatgctgctt
       61 ttttatttag ttgtttatat acttttgcaa cttatcgaac agttgatatc tattatcgat
      121 ccgcaattgc agaagcaatc gctcttacga ttatcccagc tgttttatat tatgcttatg
      181 gattgatatt caatagagaa aaaaaagtca tcgctttatc tgtcagtata agtctacttg
      241 tgtatactca tgtcttaact acactattgt gtagtgcatt gattgcttgt atgattatag
      301 ttcgtttgtt tttaagcaag caaataaaaa acgatataaa atttgtggtc gtgaatttag
      361 taaaggctgt ttttttgact tgtgcattaa ctagctattt ttggtatcct atgttgcagc
      421 agatgaatta tcaagaaata cacagtccct tcaaaacaga tttgcagcta gaagcacttt
      481 ctatttcaga tagtatcatt caagcgttaa ataacgatat tacaacatac actatggggt
      541 tattaggatt ggttgcgttg atttttcctt tattcatact gaaagaaatg gagaaaaaag
      601 aaaaaatcat ctatgttggc gtagtttcta catgggtatg cgtaactaat ttatttccat
      661 ggtttttgtt tcaaaaaaca ccgataagct tactccagtt tccttggcgt atcttaggta
      721 tccaaatctt tttttcttca atgatcataa cgcttgtttt tatgaaaaag actatgaata
      781 aaaaatattc atggttaatc attggcgcta ctgtggtgtt tctactcgtc acgtctatag
      841 cagcaaaaga aaattacgca aaagttatcc aatcaaaaca cggtcatatt gttatcaatg
      901 aagaaacact cccacgttat acaacatcag atatgggagg attatacgat tacgccccaa
      961 cagaagcgat aaaagaaagg cctcacttag aaaaacatga agtaaaagta ggagaatcgt
     1021 gggaaaaagg gaagtataaa gctgcagata ataacattac ttacacagtg gcaagcaaca
     1081 aaaagcaaaa agtgacagta ccagtatatg cttatttagg tacacaggta aaagtaaacg
     1141 gtaaagtcgt aaatgactct tataaaaaac atggcttagt gaatgtggat gtaaatgctg
     1201 gagaaaataa gatagaaatt acaacaaaat atactccaac tgctctcatg gctttatgga
     1261 tcagtattct gacttattgt ttagtggtgt attcaagttt taaacgattt gcacctaata
     1321 agttgaaaga ttctcgatat gtgactaaaa aggagataaa caagaagaat gaaaataaat
     1381 aaaaaagtag taatattgag tggattaatt atagttagta gtatatattc tttcatcttt
     1441 ttcaaaaata cacagagtat ttataccact ggagatcttt cttttcactt atctcgaatc
     1501 aaggggttat cgactatttt taccagtccg attaattatg agacattcaa ttatactgga
     1561 tatggtgtaa attattttta tccctttctg actttttttc cagctgtaat gttatattgg
     1621 atgacaaaaa atttgattgt aagctatatt atttacgttt ggttattgaa cttgtgtaca
     1681 acactcgtag cttatcatta tggcgagcgg tttttaaaac aaaaaaaagc ggcattttta
     1741 tttagttgtc tctatatttt ctctgcttat cggacagtag acatctacta tcgttccgca
     1801 attgccgaag ctatcgcaat aacacttgtt atccctgtat tattttatgc ttatcaaatc
     1861 atctctggaa aagaagaaaa gtatccgtct gtaaagctag cattgagtat gagtttgcta
     1921 gtgtacagcc atgtactatc aacattaatg agtacagcat taattatcat ttttattttt
     1981 attcgtttgg tttctaaagg tttcaagaat gcagatttta ttgctatatt taaaaaatta
     2041 tttagtgcgg caggaatgac attggttctg acgagtgctt tttggtatcc gatgtttgaa
     2101 caaatgctct accaaaaaat caacaaacct agcgtgacaa atttatatgc tcatgcatct
     2161 aatgtgttcg atagtctgac tgaagctatg aacaacgact taactactta ctcaatggga
     2221 ctagttggtc tgttatcttt atgtattccc ctgattttat ttaaaaaatt aacaaacata
     2281 gaaaaaaaga ttt
//
LOCUS       DARDHA010000234         2233 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000234 DARDHA010000000
VERSION     DARDHA010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.239"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..544
                     /locus_tag="SDA10_002747"
     CDS             <1..544
                     /locus_tag="SDA10_002747"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG4241608.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="SDA10_002748"
     CDS             complement(900..1472)
                     /locus_tag="SDA10_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG4241609.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /locus_tag="SDA10_002749"
     CDS             complement(1488..2093)
                     /locus_tag="SDA10_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HEG4241610.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          701 a          454 c          338 g          740 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tttgcacagt cta
//
LOCUS       DARDHA010000235         2225 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000235 DARDHA010000000
VERSION     DARDHA010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.240"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..241
                     /locus_tag="SDA10_002750"
     CDS             <1..241
                     /locus_tag="SDA10_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HEG4241611.1"
                     /translation="LGTRQNGREQSKNIINANLNENEIETALNKIQDPQFIKSLKNVK
                     NIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            214..891
                     /locus_tag="SDA10_002751"
     CDS             214..891
                     /locus_tag="SDA10_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEG4241612.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            881..2023
                     /locus_tag="SDA10_002752"
     CDS             881..2023
                     /locus_tag="SDA10_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEG4241613.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            2036..>2225
                     /locus_tag="SDA10_002753"
     CDS             2036..>2225
                     /locus_tag="SDA10_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG4241614.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEIN"
BASE COUNT          824 a          287 c          453 g          661 t
ORIGIN      
        1 tttaggaacc agacaaaatg gacgagaaca atctaaaaat attataaatg caaatctaaa
       61 tgaaaatgaa attgaaactg ctttgaataa aatacaagat cctcagttta taaaaagctt
      121 gaaaaatgtt aagaacatct acggtgacgg caacagttct agaagaataa caaaaacatt
      181 gaatcaaata ttgaatgatg gggaagatat taagtggatt caaaaacaaa tagcttatta
      241 aaatatacta gtgatccata cattattatt ggggctggcg gacacgcaaa agtcattatt
      301 gaaaccttaa gattagaaaa taagttaata tatggatttt ttgatgataa gcaaaataaa
      361 atgcaagatc tgaaatatct tgggaaaata aacgaattga atagctgggc ggatagcaaa
      421 aaaaaaatct ataaatttat tgtagctatt ggtgactgtg tagtaagaaa aaagattata
      481 aaaaaattga atatgccaag tgagcaatat ggaatagctg ttcatccaag tgctatagtg
      541 gcaaaagaaa tttcaattgg ttatggaact gttatttttg cgaatgctgt aattaaccca
      601 gatgctgtaa ttggagagca tgctataata aacacaggta gcattgttga gcatgattgt
      661 agaattaata actatgttca tttatctcca ggtgtatgct tagcgggtgg agtacatgta
      721 ggtgtaggaa ctcaaatagg aattggttct caatgtatcc agatgaagaa aattggatct
      781 tggtgtatga taggcgcagg gagcactatt gttaaggata ttccctcaca ttccttagct
      841 tatggcaatc cagctaaaat taagaaagaa ggaattaatt ttgaattcta gaattttatt
      901 atcaagtcct catatgggag gaacagaaga aaaatatgtt gctaaggctt ttaaagataa
      961 ttggatagcg ccattaggtg aaaatgtgga taaatttgaa gaaagtatta caaaatatac
     1021 tggttctaag ggcggagctt ctgcgttaag ttcaggtaca gctgccatac atcttgcctt
     1081 aagactgctt aatgttggaa aaaacgatat tgtctttgct ccttcattta cttttatagc
     1141 tacagtaaat cctattttat atcaaggggc tgtaccagtg ttcattgact cagaacctaa
     1201 tacatggaat atgagccctg ttgcactaga gagcgcatta gaagaagctg aaagaaacaa
     1261 ccaattacct aaggcaatca tcgtagtgaa tttatatggt caaatggctg attatgaacg
     1321 aattttagct attgcaaacc aatttaatgt tccagtgata gaagatgcag cggaatcgtt
     1381 aggttctact tataagggaa tttcgtcagg cttatttggg acaattggga tctattcatt
     1441 taacggtaat aagatcatca caacctcagg tggaggagct ctagtgtcta gtgataaaaa
     1501 atatgttgag cattcaagat ttttagcaac acaagcaaga gatcaagcac cgtattatca
     1561 acatagcgaa ataggttata attaccgtct aagcaatatt tctgccggta tcggacgtgg
     1621 acaaatggaa gtattgaatg atagagtagc taaacgaaga gaaattttca atcgatataa
     1681 ggaagctttt tctggaattg atagaattga aatgatgcca gaagtggatg gcatggtgcc
     1741 aaatcgttgg ttgtctactt taacgatcaa tgattataat tctattgatc aagtagttaa
     1801 cgtaataaat aaactaaatg aaaaaaacat tgaaacaaga gctttgtgga aaccactaca
     1861 tttgcaacca gtatttcaag gggtaaaaat gtattaccat gataataatc ttagtgtgtc
     1921 agaaaagtta tttaatagcg gcttatgctt accttctgga agtaatatga cagatagtga
     1981 acaagaattt gttattgatg agtttaaaaa aatattcaag taggtgtgat attttatgaa
     2041 cataggaatt attggtttag gttccatggg gaaaagaaga cttagactca ttcaagaaca
     2101 ctttaatgat attttactat gtggtgttga tactaatgag gcgagacgaa aggaagttga
     2161 aaaggaattt ggaataccaa cttataatag tctaacagag gcaaaaaatt tgtttgaaat
     2221 aaatt
//
LOCUS       DARDHA010000236         2217 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000236 DARDHA010000000
VERSION     DARDHA010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.241"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            189..443
                     /locus_tag="SDA10_002754"
                     /pseudo
     CDS             189..443
                     /locus_tag="SDA10_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            513..1025
                     /locus_tag="SDA10_002755"
     CDS             513..1025
                     /locus_tag="SDA10_002755"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4241615.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1022..2059
                     /locus_tag="SDA10_002756"
     CDS             1022..2059
                     /locus_tag="SDA10_002756"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG4241616.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT          805 a          412 c          450 g          550 t
ORIGIN      
        1 taataacaaa ataaaaataa gaagtcgctc actccccgac caaagtttgt gagcaaaata
       61 aacagtcgat gtttgtttct taattgtata tcttgatatt aaacgattta agattattct
      121 tcaagatata tattcgcaaa tgtaattgga aaagcgactt cttaaacaaa attaaggagt
      181 cgctttttat ggcaaaaata acgaaaaatg agcaagaaaa tttggtggaa aattacacaa
      241 aaaaaccggc gtactctaat agccggttag acgcacattt cgtgtgcact tctaatccga
      301 aattgagctt tgacgctatg acgatagtcg gaaaccttaa taaaaacagc gctaaaaagc
      361 tatccgattt tatgagttta gatccacaaa ttagactttg ggatatactt caaacaaaat
      421 ttaaagctaa agcactacaa gaccccctat gttaggctag ttgtcgtata ggataataag
      481 ttataataaa gactatacgg aggatgatta ccatgacaaa taaaaacaat gaacgttact
      541 ctaccgaact gaaagcgaca gccatcaaga aaatgatgcc acccgaaaat agggggattc
      601 gccaattaag tcaaactttg ggcattccag aagcgactct ttattcttgg cgtaaaaaag
      661 ctagaattca agggaatact acacccggaa cgaaaaaaaa tgtcgaaaaa tggtcaagtg
      721 aagataaatt tttaattgtg atggaatcat tctcaatgaa ccaagtagaa cttgcggaat
      781 attgccgaaa gaagggctta tttaaagaac aaattgaggc ttggaagaaa acgtgcttat
      841 ctgcgaatga acaggaagaa aatcgaacga gagaacttgc aactgaacta aaagaagaga
      901 aaaaacaagc gcgtcaatta gagaaagaat tacgtcataa ggaaaaagca ttagcggaag
      961 ccgctgctct cttattacta agaaaaaaag cacacgcgat ttggggggac caagaggaag
     1021 aatgatttca tcttcagatc gcaaacaagc tgttgaactt attcaagaag ccaatcgcaa
     1081 tggggcccgt ctcacctatg cctgtaatga gctgaatatt agcgttcgaa cctacgaacg
     1141 atggacgcgt gaaggtacga tagcgcatga tcaacgccct ttggcgaaac gtcctgtacc
     1201 caaaaataaa ctgacggatc aagaacgcga aaaaataata gaaaccgttt caaaaaaaga
     1261 atttatgaat ttgccaccta gccaaattgt accaaaactt gcggattgct cgatttatat
     1321 tgcatcagag tctagcttct atcgtgtatt gcgagaaaaa aatatgcaac atcaccgtgg
     1381 ccgaagtcaa gtttcacaaa aacgcatacc gccaagtcac cttgcgacga agccaaatga
     1441 agtttggact tgggatatta cttggctaaa aggtccaata aaaggactct tttaccggtt
     1501 atatctcatc attgatttat ttagccgtaa aatggtaggc tgggagattt gggaatcaga
     1561 agaagctcgt catgccgaaa aacttattaa acagacggtg cttaaggaac gtatccaagg
     1621 acagccgttg gttttacact ccgataatgg cagccctatg aaagccgcga cttttcaagt
     1681 cttactcgaa aaattaggaa ttcaaagttc ttattcaaga ccacgagtga gtaatgacaa
     1741 tccttattcg gaagcggctt tccgaacgtt aaaatatcga ccagaatatc ccaataacgg
     1801 ctttaaaaca atagatgatg ctcgaaaatg gacgaaagag tttgtcgatt ggtataacga
     1861 ttctcatttg catagtggga ttcaatttgt gtctcctgca gcgtgtcatg aaggacgaca
     1921 tctcgctatt ttagagaaac gaaagcaggt ttacgaggac gctaaaaagc gccatcctga
     1981 acgctggtca aaaggtgttc gaaattggac tccacatcaa tcggtggcgt tgaatccttt
     2041 aaaagaaaca gaaagttaac aggagcataa tctttcaaga ttatcaaata accctcgtca
     2101 cccaataggg gaaaaagtta ttcttcccct attggataac gaggccaacc gaccataggg
     2161 aggttctgct ttttgatagg tggaaaataa aaatacgaca actatattga caaacac
//
LOCUS       DARDHA010000237         2181 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000237 DARDHA010000000
VERSION     DARDHA010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.242"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..908)
                     /locus_tag="SDA10_002757"
     CDS             complement(<1..908)
                     /locus_tag="SDA10_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEG4241617.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKN"
     gene            1336..1899
                     /gene="efp"
                     /locus_tag="SDA10_002758"
     CDS             1336..1899
                     /gene="efp"
                     /locus_tag="SDA10_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEG4241618.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT          738 a          384 c          419 g          640 t
ORIGIN      
        1 atgttttttg ataaatagcg gtctgctgcg tctggaaaaa tcgtaacgac acgatgacca
       61 ggtggtaaac gacgaatttc tcttaatgct gctgcaaaag ccgcaccact cgaacttcca
      121 actaatagtc cttgttctcg tgctaactgc ctagtatagt taaacccttc tacatcagag
      181 attgtctcga tctgattgat tgaaagggga gaaagaaacg gcggaatgaa ttctacaccg
      241 ataccctcaa tttcgtgagg ggctggatca ccaccattta acacagagcc ctctggttct
      301 acaccgatta ttcgaatgtc gggatatttt tctttcaaaa aggtagaagt tccagcaaag
      361 gttccaccac tcccaattcc tgctacaaaa ctatggatat tttctttgat ttcatgaaag
      421 atttctggcc ctaacgtatg atagtaagca gcaggattat ctttattttc aaattgaaga
      481 ggcagatagc tgtttgatat ctcctctgca agttttttac ttttgctgat ggcaccgatg
      541 attccttgct cactaggaga atggatgatc tttgctccca atgctttcat caattgttgt
      601 ttttctaagc taaacttctc tggaacaaca aaaaccgttc ttaatttgta tgtgagtgcg
      661 gccagagcga tcccaattcc tgtatttccg gcagttggtt cgatgatcgt cgttttttca
      721 ttgatttttc cttgggcaaa tccttctttg atcaaataac tgcccaatct atctttgata
      781 cttcctccag gatttaaata ttctagtttt gcataaatag cagaaccttt aggcatagaa
      841 tagtgctgat tgtcaaagcg ataaagcggt gtggaaccaa tagcttctgt aattgtcgag
      901 ataatcatgt gggcctccta aaaaaagata ataaaaaaag gagctgacta ttaaatcagc
      961 tccttgaaag acatagagaa aaattgagta gataaaaaga taagttacct ggtactcatt
     1021 ctatgaaggg caattaatag acatgacaaa tagtatgctg cttttatgca ccatacaaca
     1081 gcaacgattc aattgttttt ggttgctgac tgtcatgtgt atccctccaa gtaaaatttt
     1141 tattaacgat agcatgaaaa aaaataaaag tcaaaggctt gaaagaaaaa aatatttcga
     1201 tagcatcgtt tacccgtgtt tatttttctg acaaataggc atgagacctt aaaagataga
     1261 gaatactcta gttttctggc cggttgtctg atacaataaa gttgattatg ataaagaaaa
     1321 gaaggagcaa aaataatgat ctcagcaagt gatttaagag ccggtatgac tttcgtacaa
     1381 gacggcaaac tgattaaagt tctggatgca agccaccata aacctggtaa aggaaataca
     1441 gttatgcgta tgaaactgaa agatgtccgt tcaggtgcaa catacgacac aacgttcaga
     1501 ccagaagaaa aatttgaaaa agcacatatc gacacaaaac ctgtacaata tttgtacacg
     1561 atggatgata cagctttctt catggatttg gaaacatatg agcaatatga aattcctgta
     1621 gaaacagtag aagaagaaat gaaatatatc ctagaaaaca tggaagtaaa aatccaattt
     1681 ttcggtagcg aagtcatcgg cgttcaatta ccaacaactg tcgtattacg tgttgtggaa
     1741 acacaacctt ccatcaaagg tgcaacagtc actggttcag gtaagccagc gacaatggaa
     1801 actggcttag tagtcaatgt tcctgacttc atcgaaacag atgaattgtt ggaaatcaat
     1861 acagcagaag gttcttatgt aaaacgtgcg tcaaaataat tcgctaacgg attgataaga
     1921 atttgaaaac agagcttgac ctcttgaaag gcagatcatc ggatctgcct ttcaagaggt
     1981 tttttgtgga atcagcaaat tgaaaaataa aaaagtattt gattttattt atattttgaa
     2041 agatgaatgg caaaagctag agaaagaaga aactgcactt atttatgaag aaattgaaat
     2101 gattcataaa attgaaaata tccgaactat tccgaactat attgaagcga aaatattttt
     2161 tcatcactaa atagtgttga a
//
LOCUS       DARDHA010000238         2128 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000238 DARDHA010000000
VERSION     DARDHA010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.243"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            80..>2128
                     /gene="acm"
                     /locus_tag="SDA10_002759"
     CDS             80..>2128
                     /gene="acm"
                     /locus_tag="SDA10_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HEG4241619.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     K"
BASE COUNT          837 a          334 c          425 g          532 t
ORIGIN      
        1 ggggaggaat tgaagaaagg atttttgaga gatgatatag taggttttat ttttagttaa
       61 aataggggag atagaaaaaa tgaaaaagtg tagaaatatt attttatcaa tgttatttat
      121 tattactaat ataacatcac tgattcctgt tcatgtctat gcggatgcag gcagagatat
      181 cagcagtaat gtcacttcgt tgaccgtgga cccaacgaat attacagatg gaggaaacat
      241 aaaagtaaaa ttctcgtttg acgaaaaaaa acaaaatatc caaccaggtg attatctttg
      301 gatcaattgg ccaagtgaag gaaatattcg cggtgaagga ttccaaaaag aaatcccatt
      361 aatgattgaa aataaaaatg ttggaacttt aacggtcaga aaagattctg cgcaggttgt
      421 gtttaatgaa aatattaaaa acctagactc agtagaaggt tgggggcaat ttgagataca
      481 ggccagaaac gtaaccgata ccggtgaaga aaatacggga ccattcaccg tgacgagcgg
      541 tgataaaaca gctactgtaa atgtgacaaa gccagcttct ggttcttctt ctagcgtttt
      601 ttattataaa acaggagaca tgctacctga agatactaag cacattcgat ggtttttgaa
      661 tattaacaat aacggcactt atgttgaaca accagttaaa atcagtgatg aaatccaaag
      721 tggacaaaga ttagatccaa gtacgtttga aattaatcaa atacacttag gtgaacagaa
      781 ggtttataga ggagaagagg gaatccaaca atttttacaa gatttcccca gcgctacatt
      841 taatttttca gtaactgata attacattga aataactata ccaaaaaact ttgtcaatct
      901 taggaaaata atggtgagtt ataagacgat catagaaaac ccagaacaaa taaattttga
      961 gaatcattcg gaagcttggt ttaaagaatt taataaacct gctgttgatg gagaaagttt
     1021 caatcataca gttaaaaata tttcagcttc tggtggagtc aacggtacag ttagaggcga
     1081 attgaagatt ttcaaatata tcaatgacac agagattggt atcccaaatg tgactttcga
     1141 attaagacga gcagatgaac agcctattca aggacagtct agtatcctgt tgacttcgaa
     1201 tgaacaggga gaagctagta tcaaaggcct gcaagtagga gactatgttg taaaagaaaa
     1261 agaagcaccc aattggattg attttgatcc actaagttca aatgaactca aattctccat
     1321 aaacgaaaat gatacagagg gagtcagttt acctatttat aacaagaaaa aggttacgaa
     1381 catcacagca actaaaattt ggaacggtgg aaccactcca agaccgagta tttattttaa
     1441 attattcaga gcaagtacaa acaattggga accagttcct gatgcagaaa caaaacgatt
     1501 agacaatgga ataacttcag tgacttggga agatatccaa cagtatgatg atagtggaaa
     1561 cgaatatacg tttaaagtgc aagaagtaga tcaaaacgga aatgattatg tcccttcagg
     1621 ttatcaaaaa attgaaaacg ggctagtcgt tacaaatgag aataaagaag tcatttcagt
     1681 ctcaggacaa aaaacatggg aagacaatga gaaccaagac ggcaaacgac cgacgacgat
     1741 cacagtaaat ctattagccg acggtcaacc gatccaacac aaagaagtaa gtgaaaaaga
     1801 tgattggcgc tatcgtttca caaatctgcc aaaatacaga gacgggcaag aaatcatcta
     1861 tactgtaacg gaagacaacg tgcccgaata cagtacgacg attgacgggt ataacatcaa
     1921 aaacagttat acaccggaaa aaacaaatat atctatacca ggtagccata taacaccgtg
     1981 gaaaaatttt ccagagaaga cagagagaga gaaaattaga aatctattct ctcaaaaatt
     2041 gcaaatattt gctcatacag gagaatccag cgaacaaaga agcaattata ttagtaagag
     2101 agctgtacct aaacagataa caaataaa
//
LOCUS       DARDHA010000239         2071 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000239 DARDHA010000000
VERSION     DARDHA010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.244"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            96..614
                     /locus_tag="SDA10_002760"
     CDS             96..614
                     /locus_tag="SDA10_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241620.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            622..1662
                     /locus_tag="SDA10_002761"
     CDS             622..1662
                     /locus_tag="SDA10_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEG4241621.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            1911..>2071
                     /locus_tag="SDA10_002762"
     CDS             1911..>2071
                     /locus_tag="SDA10_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG4241622.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT          725 a          248 c          424 g          674 t
ORIGIN      
        1 agtatgttat tgtttatagg tgtatgctgt tatcgtatgt tatttaaaac atctttgttt
       61 ggggattgtg ctgttttaat tgcactatat gtcatatgtt atacactgaa aaaaacgata
      121 ataatatttt tgagagacac agaatcatag gaacaattgt atgtataaca gttataccac
      181 ttctcatatc cataggaata cttttcaaat ttataccaaa tgcaatggtt aaccagatgg
      241 ttcatattgg ttctccattg atggttatag caagtatatt tttattttat gtatttaaaa
      301 atttttcatt ttacagtaag tatataaata aacttgcgag aaccagttta ggggtatata
      361 ttttacatga aaatcattat ttcattgatg gtatgaagtc tagaggctta atatgggatg
      421 ggatatttgg attaggcaaa aggtttttag atagggattt ttggatttgg tttataattg
      481 cagttgtagt tgtgtgcata atttgcatgg ctatcgatgt tgtaagagaa aaatttgaaa
      541 gacagatttt taaaattaag agtgtaaaga acctgtgtaa acttatgaat catgttataa
      601 atatataccc ataattaata aatgaggaga aaagaagtga aagttatatc attcggagaa
      661 ttattattga gacttgcagc acccggttat tcaaagttat ttcaaagaga tagtttggag
      721 actacattct gcggagggga agctaatgta gcggtatcct tagcaatatt tggatatcaa
      781 tcagaattta taacaaagct tccggattca gatgttggat atgcggcagc taatgctttg
      841 cgttattttg gagtaaatgt tgataatatt atatttggag agggacgcat gggactgtat
      901 tatttagaaa aaggagcagc tcagagacca tctaaagtaa tttatgacag aaaattttct
      961 gcaatttcgt tagctgatag tgatgaattt aagtggaagg agatcctaaa cgatgcaaat
     1021 tggtttcatt ggacagggat aaatccagca ttgtctgaga atctctttga gacgtcctta
     1081 gatgcatgtc aggctgccaa aaaaagaggt ataacaattt cttgtgattt aaattatcga
     1141 tccaagcttt ggacatcaga ggaagcaaaa gaaaagatgg aacagatact accattggtt
     1201 gatatttgta tttgtaatga agaagatgca gaaaaagtgc taggaattaa agctgagcat
     1261 acaaatatta atagtggaga attaaataaa gatggctatg tgcaggtggc tagggagatt
     1321 cacaagaagt ttggatgtag gtatgtagct acaactctgc gtagaagtta ttcggcaagt
     1381 agaaatggtt ggagtgcaat gttatacgaa gcagaaaaag ataaagcgta tttttccaca
     1441 gaatacaaca tacaaattgt tgatcgcgtt ggtggtggag acagcttttc tgctggtata
     1501 atatatgcgt tatgtgaaaa tatgtccgga caaaaggcta ttgaatttgc gactgcatct
     1561 agttgtttaa aacatactat agaaggtgat tttaatcgaa ctacaattga ggatgtatat
     1621 aatttaatga atagcggtgg aaatggtcgc gtgttgcgtt agtcaacata taaagataaa
     1681 tcacagtaat ttttatggtg attatctgtc ttagtagtga ttagaataac gtgaaaataa
     1741 attgttgagc gtttatactg ttttgaaaaa taaatttaac atattgaaaa atacaaatat
     1801 atacgtatat tataaagcgc cgattgtaaa attaagctag acaactaaaa cagtcatttg
     1861 tgctacactc aaaatagttc cgaccaaaga attataagga gtgagtgcaa ataacctata
     1921 cctatcttac aacggatgaa cttgtaataa tagagtctta tttgaaaatt aaccaatctg
     1981 ttgctaaaac tgcgcattgc ttgaatcgtt caagtcaaac gatccataaa gtatatctgt
     2041 tcttcaagca aagaaaatca gccttagaat a
//
LOCUS       DARDHA010000240         2064 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000240 DARDHA010000000
VERSION     DARDHA010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.245"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..177
                     /locus_tag="SDA10_002763"
     CDS             <1..177
                     /locus_tag="SDA10_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241623.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="SDA10_002764"
     CDS             190..690
                     /locus_tag="SDA10_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG4241624.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..>2064
                     /locus_tag="SDA10_002765"
     CDS             787..>2064
                     /locus_tag="SDA10_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEG4241625.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEI"
BASE COUNT          667 a          280 c          464 g          653 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatt
//
LOCUS       DARDHA010000241         2058 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000241 DARDHA010000000
VERSION     DARDHA010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.246"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..337)
                     /locus_tag="SDA10_002766"
     CDS             complement(<1..337)
                     /locus_tag="SDA10_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEG4241626.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(404..559)
                     /locus_tag="SDA10_002767"
     CDS             complement(404..559)
                     /locus_tag="SDA10_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG4241627.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1176..1397
                     /locus_tag="SDA10_002768"
     CDS             1176..1397
                     /locus_tag="SDA10_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241628.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1398..1655
                     /locus_tag="SDA10_002769"
     CDS             1398..1655
                     /locus_tag="SDA10_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241629.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1791..>2058)
                     /locus_tag="SDA10_002770"
     CDS             complement(1791..>2058)
                     /locus_tag="SDA10_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEG4241630.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          678 a          462 c          314 g          604 t
ORIGIN      
        1 ttttagcata ttgaaatttt tctggattat ccatatgagc aaaatatcgc accatacctt
       61 ttgcattagc acatttttgc gggtttggag accgaagctt ttgagttatc tcacgtattt
      121 gatcatacgt ctttttaccg ctaaacatta tcaaaacatg ccaatgaggc tttttcaatt
      181 caccgtcagg attcacatct ttatcatgaa gtggactctc aacccaagga acatgtaact
      241 catctaaaaa ctccctccaa ttttcaggag ccgattcagg atagactaca aacgtccaat
      301 tccttgtgcg ttcatcttta gtttttctac tcgtcatgct ataattacct taattgaaca
      361 acagtctaac agcaaaagca gtgcgccaac actaatgctt ttgctatttt ttttgctcat
      421 tttcctttcc gttagcatta acccaaattg taataagacc agacttacta agacccattt
      481 gtttcgcaat tttatcaaga ttatctgcca cttccttagt aagcgttaca acgactctct
      541 ttttttcatt actgatcata attcgtatcc tccaacgact ttttcttaaa aaccatagta
      601 acaaaaaagt aactaaggtg caacagttta gatactaatt agcgaataat aggccttaaa
      661 cccttggcat tactgatttt gtcaaaaaaa aacgctgaaa cccttgggag agtaaggcta
      721 aacagctatc gaccaatgac caatgaccaa taaggggggt gtcgtagtcc ccccctcctc
      781 cgcccaattc ataaacacca aaaccaaaaa acaaacctca gggctccgcc ccgaaccccg
      841 acctccttct accgaaatat atgcaggcgc cacgcttact atttcgttca gctatcggct
      901 tttgcaatct aacacgaccg tttcgctacc agtcgcctta caggctccat tctcgcttct
      961 cttacgtgtt ttaacgattg cgccgataac taaacgaaat agacgctaaa tcgcttaaaa
     1021 cgcaaaatag acccttttta gagcattgat tttttctact agtcatttcg ataatataaa
     1081 attgaccaca acacagaaaa cttatattat aagcttatgt cgtaagtccg acgtactcac
     1141 gtaatataga cggacgttaa atggaaggag cataaatgag agtgataaaa tcagaaaaat
     1201 tcactttggc cgaaattatg acgaccagcg aacttgcaca agaacttgat tacaaccaag
     1261 cttacgtttt gcgcttagcg aaagaacatc tgactgaagg aaaagaatat cgctctgccg
     1321 gtagaagaaa ctatcttttc agcccagaag cccttaaaaa actagaaaaa gtcataacca
     1381 ccaaagaagg tgagtaaatg cctaaagaaa caactatcaa cgttgaacaa ctagaaaaat
     1441 tgctagaatc atattccaat gattttatcg aattgaccgg cggaaaagtc gaagaaacca
     1501 ccgacctctt tgacctactt ctcacaaata ttctggaatt aaactctgaa gaacaaaaag
     1561 agcataaggg aaaactgata gcaccttcct acaggaaggt ggctaagtcg gctgtgccaa
     1621 ctggttttct tacaaaacaa ccttatattt tttgatatca caaaaaaatc cattttggac
     1681 ctattttcgg tcataatata gtacctactt ttggtcatag tttcgtccgt agttttggtc
     1741 atagtgacta agactttcac gcacaaaaaa agagctgaaa ttaacagctc ttatccttta
     1801 tttatttttg aatttacact tccaccatga cgattcgatt ttatatattg cccgataata
     1861 taagctgaat tatcacaaag aagcggaaac caatcatcaa atctttctga cattgcatag
     1921 tcaattaaat ctttgatttc agtgatattc ttagaatcaa caaacgacat catctctcta
     1981 atcaactcat atcgttctgc actcgttact gacaaataac tagcaatttc tgcaccacca
     2041 tgagcgataa tatcactt
//
LOCUS       DARDHA010000242         2042 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000242 DARDHA010000000
VERSION     DARDHA010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.247"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..153)
                     /locus_tag="SDA10_002771"
     CDS             complement(<1..153)
                     /locus_tag="SDA10_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEG4241631.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
     gene            complement(475..696)
                     /locus_tag="SDA10_002772"
     CDS             complement(475..696)
                     /locus_tag="SDA10_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241632.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(794..1039)
                     /locus_tag="SDA10_002773"
     CDS             complement(794..1039)
                     /locus_tag="SDA10_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241633.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1279..1893
                     /locus_tag="SDA10_002774"
                     /pseudo
     CDS             1279..1893
                     /locus_tag="SDA10_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          678 a          364 c          298 g          702 t
ORIGIN      
        1 tacccaacga taaatcgtag tatgacaaac atttattcca cgatcatata acaattcctg
       61 aacttcacga tagcttagat tgtaacgcag gtagtaacca acagcgacaa taatgacgtc
      121 ttttttgaat tgtttgcctt taaaatgatt cattactctg tcctctctgt cttttttctc
      181 aattttacac taaaatagat tttttggaaa actttgcaac agaaccgttt ttactatata
      241 cttcttccca gaacttgtcc gtctccttca aattatctct acctctcgag ttcattgggt
      301 ggtttgccca tttattaaac atggaatcac ctttctcata ctcatatatt tatgtcctat
      361 ttcaattata tttatatatg cttaaataaa tttgtatcct gaaatttatt gtaatatgaa
      421 gctgtatttt acatatagta accaaagagt taataaaata aaaagagcga accattatag
      481 atcagctctt ttttcattgc tgtttttaat cttatctaga atcacttcaa atgtttcaaa
      541 ggtatttaca tctaactgac ataaccaaag caagtacttc ttttctttgt aagtccaatc
      601 aatattacgg taagaggaca ttagattatg gtaccttgct ctttgttcaa ctgaagccaa
      661 ttcccagcgt ttttcaaata ttttttcttc ttccattatt gatccctcgc aagacgctac
      721 ctcaaactta aataatgggc agcgaaagtc taattcctta gatctaagta tagcaaacaa
      781 atatgaaaaa aacttagaag gtagtaaaag ttcttccgtc ttttgctttg cttctattct
      841 taatgtgttt cgttatcggt gccctacaaa gattttcatt aattacacac tcatttattg
      901 ttgtgtattt tctgacattt ctctcttgat caattatttt aattccatgg gcatcctgat
      961 agttcatagg agaggagcgg ctatcagaaa tagttgcacc tatctcttga acttctttta
     1021 caacttctgg aaaatccatc catcctgctt cagctgtcaa aagtaataat ctataatatt
     1081 tatctttcct tttcaatcta agaaatcgtc accccattct ttttcttcta ttataaacta
     1141 tttaactttt agaattcaaa aaccgtttgt ctatttagag tttaccctaa atagacaaaa
     1201 atataacatt gaaaaattta gagttctaac gtatgattag ggtgtattat aaacgttata
     1261 gaaaagtagg gaataaattt gaaaattgct tatattagag tttcatccat cgatcaaaat
     1321 gaacaacgtc aaattgaaga aatgaaaaag tttggcgctg aaaaaatttt tatcgaaaaa
     1381 caatctggag cgacgattag tcatcggccg attttccagg aagctttaga ttttgtcaga
     1441 gagcaagata tttttattgt agaagccatt gatcgacttg gacgcaatta tgacgagatt
     1501 atcgattcag tgaattatct aaagaagaag aatgtgcgac ttattatcac cagtttaccg
     1561 atcatggctg aagcaattgg aaatcctctc ctagataaat tcatcaaaga cttaatcatt
     1621 taaatctcag ccatgattgc tgaacaagaa cgtactgaat cgaaacgacg ccaggcacaa
     1681 ggaattaaaa tcgctaaagc aaacggcgtc tataaaggtc gtcctaagtt atacagtgca
     1741 gacaccaaag atcctcaacg tcgccttgtc tataggagta tcgttcagga tcttgagaat
     1801 ggggttgcta tctcaaagat tgcaacagac tacaatgtga ctaggcaaac aatttaccga
     1861 ataaaaaaag aaatcgacca actaattgta tgatttcttt tttgaatcac gtaattatta
     1921 attagctttg tcatcaatac agacaatcat tttagattca tctagctaag ttgattacat
     1981 ttatgttaaa aagaatttag ttaacataaa tataattgat gcataaatcg tccttattta
     2041 aa
//
LOCUS       DARDHA010000243         2033 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000243 DARDHA010000000
VERSION     DARDHA010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.248"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(555..920)
                     /locus_tag="SDA10_002775"
     CDS             complement(555..920)
                     /locus_tag="SDA10_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241634.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(996..1241)
                     /locus_tag="SDA10_002776"
     CDS             complement(996..1241)
                     /locus_tag="SDA10_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4241635.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1311..1565)
                     /locus_tag="SDA10_002777"
     CDS             complement(1311..1565)
                     /locus_tag="SDA10_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241636.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1729..1941)
                     /locus_tag="SDA10_002778"
     CDS             complement(1729..1941)
                     /locus_tag="SDA10_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241637.1"
                     /translation="MIFQGVITLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIIL
                     SFLVNAIVLFLYVLNYLDINPLIFSR"
BASE COUNT          749 a          352 c          366 g          566 t
ORIGIN      
        1 gtctgaaaac aaaaaaagaa agcgttatta ttttagtcta ttttcagggg gggaacctta
       61 tgataaaaaa actattttta ctatcaatat taacgctagc aatggcaggt tgttctgact
      121 ctggcttagc aactgaactg atcacaaaat caactgaagc cacgacagag gagttaaaat
      181 gtggaaacga tcacagtgac tacagaggct taaatcgacg atgcatggga atattaggca
      241 gaagcaattg taagcatcga tgtagtaatc gaagaaggtg tagaagaagc aacagaaaaa
      301 tcaaaagaaa gatcaaaaga agcaatctgt gctgtgcatc gtatacagac gtcgaagatg
      361 cggtactttt aaaccacgtc aggagatgtt catttatgtc tagggaagtt ggcgatgaac
      421 caaaactaga gtacgctttt agttttgcac gtgaggatga agataaccct aaaacttacg
      481 acattttagc ttgatctaag atggttaaag ctttttaatg aagtaagagg agatagaagg
      541 cttttctagt tatattaggt aagataaatt cctttttgct ccaaatttgc taaacattct
      601 gctagataaa cttcatcgtg ttccggataa atcggcacgt tgcgctctac agtaggtaaa
      661 tcaaaaaatg cttcgcatga tttgccacga tacataggat ttttccactc tggtgctggg
      721 ctgtaaccac gtttttccat ttcatccatg acaagctgat gatattggaa taatctataa
      781 ggagaatagt gaaaaacata atccacagta gcatgtttct ttccccagcc atttcctctt
      841 aatgcacagc attcccgatg ttgtcctaaa agctgttgac gaggaagctt tgaaatcaat
      901 ttttcatgcc ataatcgcat agaaatgtct cctttatata tagattgtta accttagacg
      961 ttaaatgaaa aaaccagcac caaggctgga aaaaatcact tcagtttgct tgctgtttct
     1021 gagcgaccaa ttaggtaatc aacggaaata tcgtaaaagt cagcgatata gattaattca
     1081 atgatgcttg gaatttcttc tccttcttcg tacttacgat atttgaataa aggaatactt
     1141 aacgcgctag aaacagctac ttgagataaa gatcggccag ttcttaagaa attcagacga
     1201 caacgtaaag tatccagtaa aatagcttct tcgatactca tttaatcacc tttcattata
     1261 gtagaatagt tataatgact atagaaagct acacatgcaa tcaatagtac tcaatgtttt
     1321 ttatttaaac gtatcaaata aagaatgacg atcaataaag acaaaaccag tcctgataat
     1381 tcttctataa aaacactatc gggttcaaag tagttgtaat taaaaacttt ccacataaag
     1441 tagatagccg gaagagtcaa aatagctaaa taaaaccgtt ctttgttcca attcataaga
     1501 taaacaatac ttgcgaccag tgcgaccgca ggcacccaaa acaaaactct caccaacgta
     1561 atcatgtaac ggactcctca acgattcata taataaaaga taacatgtta ttgcatacat
     1621 cacaaatgaa atgctaggaa aataaaagaa gaatagatca atgaaaaatg aaaaagcaga
     1681 acagttttct gctgataagc aggaactgtt ccgctgaatg tgtcttatct atcgagaaaa
     1741 aatcaatgga ttgatatcta agtaattcaa tacatataaa aagaggacga tcgcattcac
     1801 taaaaaggaa agaatgatga tcccatagcg aatggaaaca tatttaacaa atacttttgt
     1861 ttgaatacgg gcatatcttt ttcctttata gctgagcatc atgacaagaa gcaaaagggt
     1921 aatcacacct tgaaaaatca ttccaataaa cagaattcct gaggtttgat aatttaattg
     1981 gagaaaataa gctgctgccg caactaaatc gaataaaatc gcaataaatg gca
//
LOCUS       DARDHA010000244         2003 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000244 DARDHA010000000
VERSION     DARDHA010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.250"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..378
                     /locus_tag="SDA10_002779"
     CDS             <1..378
                     /locus_tag="SDA10_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEG4241638.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="SDA10_002780"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="SDA10_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEG4241639.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="SDA10_002781"
     CDS             1859..>2003
                     /locus_tag="SDA10_002781"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241640.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DARDHA010000245         1992 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000245 DARDHA010000000
VERSION     DARDHA010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.251"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            274..840
                     /gene="amaP"
                     /locus_tag="SDA10_002782"
     CDS             274..840
                     /gene="amaP"
                     /locus_tag="SDA10_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEG4241641.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            853..1041
                     /locus_tag="SDA10_002783"
     CDS             853..1041
                     /locus_tag="SDA10_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEG4241642.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1054..1611
                     /locus_tag="SDA10_002784"
     CDS             1054..1611
                     /locus_tag="SDA10_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG4241643.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1643..1885
                     /locus_tag="SDA10_002785"
     CDS             1643..1885
                     /locus_tag="SDA10_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG4241644.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          725 a          308 c          406 g          553 t
ORIGIN      
        1 aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt aatcttgaat
       61 aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa gcaattgtta
      121 tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg gtagcgcttt
      181 atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt agagttattt
      241 acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa aataatcggt
      301 gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat caatgcgccg
      361 tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa ttactacatg
      421 caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat catcttgcta
      481 gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga agatggcaaa
      541 ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca cgaagaagag
      601 ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca tatccatgtc
      661 aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt gatgcaagat
      721 atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt ggcagtcaca
      781 tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg agtggaatag
      841 gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat ctttgggggt
      901 cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac attgttgatc
      961 ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc aggaatagtt
     1021 gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat gaatacaaca
     1081 aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg tgaattgact
     1141 tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt agatggtcta
     1201 ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa cacagataac
     1261 gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga tctagatgtg
     1321 atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga attgatcagt
     1381 actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa tgttgcagac
     1441 atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa agtaacagaa
     1501 gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa atctgctgta
     1561 ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata agaagtaaga
     1621 agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg gcggtatcat
     1681 tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca tcgcaaacat
     1741 cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt ggggaccaac
     1801 aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag tattagtcgt
     1861 ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg aaatgaagaa
     1921 caaatttgag aaccgtagcg acactcgctg cggttctttt ttatatattt tatatgaagt
     1981 attctttatt cc
//
LOCUS       DARDHA010000246         1921 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000246 DARDHA010000000
VERSION     DARDHA010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.252"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1008
                     /locus_tag="SDA10_002786"
     CDS             <1..1008
                     /locus_tag="SDA10_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241645.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="SDA10_002787"
     CDS             1025..1174
                     /locus_tag="SDA10_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241646.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="SDA10_002788"
     CDS             1171..1617
                     /locus_tag="SDA10_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241647.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="SDA10_002789"
     CDS             1619..1756
                     /locus_tag="SDA10_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEG4241648.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
BASE COUNT          689 a          337 c          401 g          494 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 g
//
LOCUS       DARDHA010000247         1918 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000247 DARDHA010000000
VERSION     DARDHA010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.253"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(97..798)
                     /locus_tag="SDA10_002790"
     CDS             complement(97..798)
                     /locus_tag="SDA10_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG4241649.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(814..1254)
                     /locus_tag="SDA10_002791"
     CDS             complement(814..1254)
                     /locus_tag="SDA10_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEG4241650.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(1254..1829)
                     /locus_tag="SDA10_002792"
     CDS             complement(1254..1829)
                     /locus_tag="SDA10_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEG4241651.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT          546 a          409 c          295 g          668 t
ORIGIN      
        1 tatttcaaat atttgaatat aaaataacca aacgatagaa aggcttattc tacatcacgt
       61 agtaattttt ctttcccgtc gtttggtttt ttatttttag cctaattctt gaccttttgc
      121 atgctggtta tccgtaaaat attttccagc aggctttcct tccaattcag ctaatttttc
      181 ttggatctga cggatcacct tttttgcatc ttctggattt tctacaaaat cgaatttatc
      241 cccatccact tgtattttag gacatacatc aaaatcttca taccattgat tatagcgggt
      301 gttcaacgtt tcataatagg tatataacgt ctcatcgctt tctagctgtt catagtcacg
      361 cccacgtttt ttgatacgtt ctagcatcgt gtccaaagaa actttgatat ggaccatcaa
      421 atcaggccgt ttttttggag caacatcatc taactcgttt agcatcgtat ctaataagct
      481 atcgtactgc tgtacttcga tatccgttac ccgacctaaa tcagcgttta gatggaacaa
      541 caggctatct tcatagatgg atcggtctag cacattgtca tcgttcacta aagcattttt
      601 cattgctaaa aatcgcttgt tcaaaaagaa gatttgcaat aaaaaagcgt actgctcagg
      661 atttgaataa aagagcggca atacttcgtt atcttcgaca ttttcataaa atggtttaga
      721 atcaatctct tttgcaagca tatccgtcaa actgctctta cctgcaccaa tcgttcctga
      781 aatcaaaatc actgacatac aacccttcct cttctataag tactcgccta cgtaagtttc
      841 aacaggcaat gtttcaaaat catattcacg tacagcttgg tctgatttta aatacgtatg
      901 gagactttgt gtaatcatca agttcgtagg taccgtctct tcttgtacaa tgatcatcgg
      961 ttttttcaac atagttgcca cacctaattc gtaagccgtt cctggatcga ttgtttgatg
     1021 ctcaaagtca aggatagcaa caacaaattc tgcatttgtc agttcttcca tatctttttc
     1081 gtatacttcc tgcgcccatc cagcgctgaa tagttcataa ttgctttctt ggtgatgacg
     1141 tggagagtag aaacttgtga ctgttttgtt ttcctccaac gctttttcga tacgacttac
     1201 tcgatcgatt tgctcctctg aaaaaaatgg tcctgctaaa tatatttgac tcattatttt
     1261 gcctccttca ataataactg atttttaata gatactgctc gtaattcatt gaccagttcc
     1321 aatggattgt tggaaaagat tacttgatcc aaccattttt tatccataaa atcctctttt
     1381 gcaaaagttt ctaataattg ttgcaagccg ctgtaataac cgccaatatt caatattccg
     1441 attggcttgt cgggcgtcag accgattgct atccaagaaa gaagttgtga gaattcctcc
     1501 aatgtaccta gacctccagg gaaaacgagg acagcatctc ccttttcaaa taataatacc
     1561 tttctttcat cgatcgtttc tgtaggaata aatgtagtaa catcttttcc aggcaattca
     1621 gtttcaaaaa gcccattcgg atagatacct gtgacttttc cgttattttc cagtactgtt
     1681 ttactaatca ctcccataat cccggagtat gaaccaccgt aaaccagttc gatttcttct
     1741 tgtgccatgt attttcctaa cgtttgcgca ataaatttgt aactttcttt attaccaaaa
     1801 cgggaaccgc aaaaaacagt aagtttcata aaaaaacact ccctaactca aaagtctatc
     1861 ttcattctac acgtgttttt ttaaaataaa agactagatt ttgtagtttt ttagtggt
//
LOCUS       DARDHA010000248         1911 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000248 DARDHA010000000
VERSION     DARDHA010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.254"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..969
                     /locus_tag="SDA10_002793"
     CDS             <1..969
                     /locus_tag="SDA10_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG4241652.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="SDA10_002794"
     tRNA            1159..1232
                     /locus_tag="SDA10_002794"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
BASE COUNT          639 a          327 c          362 g          583 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg
     1261 ttagaatttt tttgaagagt attatacaag ctaaagcttt tcttcattgc cactcaaatg
     1321 agtggctttt ttatgtatcc ttttatggat taatgaaagg atgtttcaca tagttatact
     1381 tctgtatatt tgaaaagttt tactttgatt tttaaataga aagacatttg ggttaaattg
     1441 tgagataata ataaagaaga gtttaaagcg caccccaaac cacttcccca taagtgtgtt
     1501 acgctttaaa ctcttttata tttgaagcca ttaaaaagca taccatattt ttgaaaaaaa
     1561 gtgagaaaaa aggcttacaa ttggagtggt agttaattag tgacttattt ttgattttat
     1621 agcactgata ctataaaata tagatatcat catattacac aatcttaata ctaacttaaa
     1681 aaatatctcc tttcacaagt atggtgataa aattcgttcc gggctacctt tttaggtagc
     1741 ctactttaat ctttatacct ttctggatca acgaaagtat actttatata gtcataacgc
     1801 cgatgatcgc tacgtgcgtc cggcacgtca gtcatgaatc ggctacacta gactagacag
     1861 aaaaaataag gtgtgtagaa taataaaaaa cacactggag gatttttcat g
//
LOCUS       DARDHA010000249         1901 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000249 DARDHA010000000
VERSION     DARDHA010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.255"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(441..938)
                     /locus_tag="SDA10_002795"
     CDS             complement(441..938)
                     /locus_tag="SDA10_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG4241653.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(978..1787)
                     /locus_tag="SDA10_002796"
     CDS             complement(978..1787)
                     /locus_tag="SDA10_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG4241654.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
BASE COUNT          547 a          357 c          336 g          661 t
ORIGIN      
        1 gttagagcag acggctcata accgtccggt cgtaggttcg agtcctacaa ggtacataag
       61 aggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaagtaaa aacagcttct
      121 tatgttttta cttggctgtt cttattactg cagatgcaag cttttgaata ccgtttaagg
      181 aagaggttgt gaaatcagcg ttttgacctg agtattagct taacaaacgg aaaaatagtt
      241 cctcatattt tgccgtttgt taagctaata ccgagggtca gctgattgaa caccatttaa
      301 gaaagaggtc gtgagcctgc cgtttcactc tgtgtcataa aaaagccaat ctactttgat
      361 agaaaaagca cgtaggacta aaacagttct gcgtgctttt tttattgtgt gttaaatatc
      421 gtaaatgaag atagatattt ttatgattcg acgatagaga cgttgaaatc tttcaacagc
      481 tctaaagcgg attctccgtg ttttctactg gagactaaca ttggatcaac aaaaaagtct
      541 gcttcaggaa aagcactctg caggcctatc agattagaga gtacacacaa gtttgtttca
      601 atcccgacta cttcgatctc tttttgttct tctttgcttt caaataaagt ttcctggatt
      661 tcagctaaag tttcaggtgg aatcgtaaag taatactttt taatcactcg ttcattttct
      721 tgtggagaaa gaagagggat caattgcaaa tcttctcgtt cttcttcttt gtccttcagt
      781 tcaactggta tatcccgtgt gaagagaata tattcattat tttggcgtgc tttagctaat
      841 ctttgagcaa tacgttctgc caattgatct gactctggaa tataaaattc gctgtttgga
      901 tcaagaatat gattttgcat atcaataata actaacattt tctttcctcc tacattcata
      961 tcgtcatttt attcttttta atagaagttt tttaaaatat aagcaacacc agcttcttca
     1021 ttcgtcagtg ttacttggtt tgcaatttct tttacttgtc gctgtgcatt ccccatcgca
     1081 atactcaacc cgctgtaatt caacatagca atgtcgtttt cttgatcgcc aaaagccatg
     1141 atgtcactcg attcgattcc aaggtaatcc gccagaaaaa caagtgaatg agctttagtg
     1201 atacctgtag gcgtgatttc taagaaatgc ggatcagaga atactgattg gataccatct
     1261 gcttgcagat ctctttgtag cttctgtagt ttttcttcac tcttatctgt taatgctaat
     1321 ttaagatagc taagagaagg atcttccaac atatttaaaa tttgatcaaa agaatggttc
     1381 tgtaaattga aatgttcata ataagggtca tctttgacta gatttgattc atcgaatgtc
     1441 agctgatcat cttgggtttc tcggctagca atgatggata ttttcgcttc ttgagcatag
     1501 aagaaggctt ttctcagtgt atcgctatct atttttgaac gtctgagaac tttcgccatt
     1561 tttccatcct tcatttcagc aatcagtgaa ccattggaag aaatcaaata gccaccgatt
     1621 cctaattcgg aaaaaatatc tttagcagaa atgctgttcc ttccggtagc gacggttacg
     1681 attccgccat tttcatcaaa tttccgaagt gcttggatat tttcttctgg tatctttcca
     1741 tttcgatcaa gcaaggtacc gtctaaatca atcgctgcta gtttcatttt atcttcctac
     1801 tttcttttta catatctatt ttcctgttac cttaagatta aatgagaatt taaaaaaatg
     1861 aaactaactt gctttgtcat tatgaatttt ttcaaaaaac a
//
LOCUS       DARDHA010000250         1887 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000250 DARDHA010000000
VERSION     DARDHA010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.256"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(258..1682)
                     /locus_tag="SDA10_002797"
     CDS             complement(258..1682)
                     /locus_tag="SDA10_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEG4241655.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
BASE COUNT          538 a          391 c          289 g          669 t
ORIGIN      
        1 ataaggaaag cgttttattt ttcatctgca ttttttctat tttctaatca aacttacaac
       61 catctgtttc tatttaaaaa cgttggtgtt atacagtttt gttgcaaaca tttattttat
      121 ttacacatcg tgaacggacg tacaaataat ggttttttct tagtttcttt atatcgtatt
      181 ctttttccga aaaaaaagaa acagcttgaa atttaaatcc aagctgttat attctttaat
      241 ctatttactt tgtgaaatta ttctgttaac cacttttcta gaattttcat ctttgaataa
      301 aacaacggta tttggttttc atccagccta ctgtaattga ttctgattcc atttatatct
      361 tgcccaaaaa gtagacttgg tgcaattcca agacccatat ctactacttt ttctgctagt
      421 tcggggtcta gttttttccc atcccaagta atccaggcat agtaaccacc taaaattgaa
      481 tgaagcttga aaaacgattg tgaatctccc caagcgctta tcagtcggct tttttctttc
      541 aacttttttc ttacatcttg cattttcaaa tcaattgctt cattaaatac attagccact
      601 gtgatttgtg taaggatatt catcgaaaac tccatcattt cttgcgcttg agctagttgc
      661 gagataaaag tctcaggtcc tatcacccaa ccgattttcg ttgtttttcc taaaacccta
      721 gataacgaac cgacgtataa cacattatcc ggatcaatcg ttcgtatagg aaggacttct
      781 tcttggttta gaaatgaaag atccgtaaat acatcgtctt caataattgg aagccgatac
      841 tgtcgacaca attctactag cttttctcgt cgtttttgac tcatcacttt tcctgtaggg
      901 ttttgaaaat tcgggttgat aagcactgct ttcaatttat ttttatggat cgattctttc
      961 agtttttcta agtcgatccc ttcttcatcc atcggtatcc caaataaacg cgttcctgaa
     1021 gctttgaaaa gaggtaatcg gtaaaaaaaa gaaggtgact ctacagctat cccctctcca
     1081 gctgaaagaa tcgtttgtaa aatcaaaaag attgcttgct gaccgccacc agtcacaaga
     1141 agctggttgg gggaaaatgc caattcaaat ttactttcca aataccgttt gattttgtta
     1201 cgtaacggag tatagcctaa aagatccatt tctttttctt ccgctaagat ttcttcaaga
     1261 gaataagaag gaaactcaaa attcggcaaa agctcatctg gcatctcgct agcgtataaa
     1321 tcaagaaacg atgttttttg gatctgcttt gcttctctta ttttttgctc ataccagtcc
     1381 gtctgctcat aacgaggaga gatcatttgc cgccaatcga ttcttggaac agcgaatttc
     1441 ccccattcag tctgattaac aaaccatccg ctgccacgtt tgctcgttag taccccaagc
     1501 tccctaagct cttccaatgc atggacaacg gtggaacgat tcacaccaaa aagttgccct
     1561 agttttcgtt ctgattcaag ttgacttcca ggattgatcc ttccgttttc gatttctctc
     1621 ataatctgtt ccataatttg acgataaatc ggtccttgtt ttttctctac tttttcccac
     1681 ataagcttct cttttctatt gtctattttt tcttttgatt ttaccgaatt tacgagaaaa
     1741 aagaaaaact ttttttgcaa aacataaggg actgtgacaa aaatcttatc acagtccctt
     1801 attctgaata atgatgttca aaagcttgta tctgcgataa taattccagc caaacaaaac
     1861 atataaagaa ctatttttgt ttggctg
//
LOCUS       DARDHA010000251         1719 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000251 DARDHA010000000
VERSION     DARDHA010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.257"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            4..>1719
                     /locus_tag="SDA10_002798"
     CDS             4..>1719
                     /locus_tag="SDA10_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEG4241656.1"
                     /translation="MKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGSSR
                     DKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGACTD
                     GTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISSRS
                     FGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWDNS
                     FGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGKLG
                     FNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKGII
                     SEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQAIT
                     LVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFEVE
                     KYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIVWP
                     ASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLMGL
                     SEFKGI"
BASE COUNT          622 a          221 c          343 g          533 t
ORIGIN      
        1 actataaaag tagacttacg aaaaaaacca tatcatttag atgaagatgc tatttcttgg
       61 attgaagaga cgattgatgc tatgacttta gaagaaaaaa taggtcaatt atttgttaat
      121 atgggatcta gtcgtgataa aaattactta agtagtatgg tcaatcagta ccatattgga
      181 gctgtacgat ataatccagg aactgctgat gaggtatatg atcaaaacta tatcctacaa
      241 acagaaagca aaattccttt attaattgct gctaatacag aagcaggtgg taacggagct
      301 tgtacagatg gtaccgaagt tggtgtagaa gtaaaaattg gtgcaacaaa tgatgcgaaa
      361 tatgcttatg aattagggag agtttctgga attgaagcat cggctattgg atgtaactgg
      421 agttttgctc ctattgtaga tattaataga aattggagaa atcctatcat ttctagtcga
      481 tcttttggac aaagccctga taaagtttta gaacttagtt tagcttatat gaaaggtatt
      541 caagagagtg gaattttgcc tgcagctaaa catttccctg gtgacggtat tgacgaacga
      601 gatcaacatc tatcttttag tattaatagt ttaagtacag aagaatggga taattctttt
      661 ggaaaagtat actcaggctt aatagaagct ggattacctt caattatggc aggtcatatc
      721 catatgccag cttattcaaa acattttaat cttgatctga aagatgaaga tttacttcct
      781 gcaactttat caaaagaatt aatcactgac ttacttagag gaaaactcgg gtttaatgga
      841 ttagtagtta ctgatgctag tcatatgtta ggaatgactg gggcaatgaa acggtcagaa
      901 ttattgccca cttctattgc ggctggatgt gatctattcc tattttttaa tgatccagat
      961 gaagacttta attatatgat ggaaggttat tacaaaggaa ttatttcaga agagcgttta
     1021 cgtgatgcat taagaagaat tttaggctta aaggctaaac taggtttgca taaaaaagaa
     1081 aagaataaaa ttttgttacc gaaacaagaa gctatggaaa aaattggttt aattgaaaat
     1141 aaatcaattt ttaaagaggt agctgatcaa gcaattacac tagttaaaga taaacaagat
     1201 atttggccta ttgtaccaga aaaatataaa aggattttac tggtagatgt acatggggta
     1261 aaaggtgggt ttggtgaagt agttgctgga aatagacctc aggccattga tttattgaaa
     1321 cagattttgt cagcaaaagg atttgaagtt gaaaaataca tttcacctga agaagaattg
     1381 aaaggattat cgcaagaaga aaaaattaaa aatatagctg gaatatatgc tgctaagaga
     1441 ccaatcaaag atttaactga aaaatatgac ttgattatta atatagctaa tgttaaccca
     1501 ggtacagttc aaagaattgt ttggccagca agtaaaggta ctccggatat tccattttat
     1561 attcatgaaa ttccaacaat ttttgtatct gtacagtgtc cgtttcattt agctgatgtg
     1621 cctcaagtaa aaacttatat caatacttat gatggaaaac aagaaacttt agaaatatta
     1681 gtggacaaac taatgggcct ttctgaattt aagggaata
//
LOCUS       DARDHA010000252         1713 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000252 DARDHA010000000
VERSION     DARDHA010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.258"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            190..564
                     /locus_tag="SDA10_002799"
     CDS             190..564
                     /locus_tag="SDA10_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG4241657.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(678..1307)
                     /locus_tag="SDA10_002800"
     CDS             complement(678..1307)
                     /locus_tag="SDA10_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG4241658.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1441..1513)
                     /locus_tag="SDA10_002801"
     tRNA            complement(1441..1513)
                     /locus_tag="SDA10_002801"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1478..1480),aa:Thr,seq:ggt)
BASE COUNT          595 a          251 c          288 g          579 t
ORIGIN      
        1 ttttcttcta gtctacacac cttaattttt ctgtctagtt aattgtagcc tatccaagtc
       61 tacactctga gatgtaagaa atatcataag atatttctta catctttttg ttttgagcag
      121 gactttagat aattttatta atatatgcta gcatgctttt gtaagaaaaa gaaagagggc
      181 gtattagtta tgtatcttaa aattacggaa caagcacaag aaagattaca aaaatatatt
      241 gatgaaggcg caacagttat attggattta gatgatggcg ttggggaata ttctaaaatg
      301 ggtgtttgct ctttagatac tagttttcgt ttacttcttc ttgataaaga acagagaaaa
      361 aatgattata aactccaatt agatagtgat attggggatg tttatataaa agattattca
      421 aaaatgtaca tggatgaaca tatgaccctt tctttagatc cacgtctagg tgtatttact
      481 cttgaaagcc caagtggttc tttagacagt catgttcaat tagtagatct acgaccagaa
      541 aaattctctg gaataaaaca ataactttct ctctattgtc cataaaacta gtctcaatca
      601 agctgtattg aaaaaacaag acaacattct taccatggta aataaaaaga cgacacattt
      661 tgtcgtcttt ttttaaatta actattttcg ataaaagatc gcatagcttg tgccattatt
      721 tctggctttt ctctattcaa ttcatgtttt ccgtttttaa taaagagtag ccggccattc
      781 gttgaaagtt tatataaacg ttttgctgct ggtttattcg catgatcttt ggtaccgcat
      841 aaaatcaatt taggaagtcg tgatgtacga agctgttttt tgcagtctaa tgtttcaagt
      901 gaacgaagta gggtaagggt ttgtttttta gatagaccta atttttcaaa tatcttagaa
      961 ggtacgcagt aaaaataaat attctggaaa gtaagcaata agcgattagg agactcaaat
     1021 tgtggagcga ctaaaacaac cttttttaaa tatggagaag gagttgcaag atagtttaaa
     1081 gcaataacag cgcctaaaga taatccacat aaaataaaag gttcctttat ctgattcaat
     1141 tgtacttcga tgtatttggt taagtcgtct aaagtagcag tgtctgtgct gtttaaatga
     1201 ttgaacaatg aaagactttt gcaagaataa ctattgggta gataagagat tacatctttc
     1261 catgattgtt cgtcttggcc tagaccatgc aaaaaaacaa ttttcatatt gctctccttt
     1321 ttatagttat catatagact ttctttgtac tattaaaata taaacattca tttcttacgt
     1381 cataaaataa aaaaacagcg cagacacatt gttaaaccaa catgttgcac tgtttaagga
     1441 agccacttgc cgggcttgaa ccggcgacct cttccttacc atggaagcgc tctaccagct
     1501 gagctaaagc ggcattatca ttaatacaaa taataggata acgaatatta atgtatttgt
     1561 caacagaatt ttttcgaaaa aatgtgtttc tctaatagga aaacagattt tagaaaaaat
     1621 aaaaataaca aaaaatgcga acggtttctt tgataatata ataaaaaaat tcaaaaaaag
     1681 tattgcgtta tttagaagaa attgatatta tat
//
LOCUS       DARDHA010000253         1699 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000253 DARDHA010000000
VERSION     DARDHA010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.259"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(139..516)
                     /locus_tag="SDA10_002802"
     CDS             complement(139..516)
                     /locus_tag="SDA10_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241659.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(816..1697)
                     /gene="galU"
                     /locus_tag="SDA10_002803"
     CDS             complement(816..1697)
                     /gene="galU"
                     /locus_tag="SDA10_002803"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEG4241660.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          477 a          378 c          247 g          597 t
ORIGIN      
        1 atcccgatac aacaagcatt cctttaaaaa agattctttt atataagtga aaaaagcttt
       61 tttccaccaa ttaaataaat ctctcatctc aatagtttat ccattatttt atcttttttt
      121 gcgtatacct actaaatatt accgtcttct tctcgttctt ctactttgtt cgagagaatc
      181 tgtactagaa aaagtagttg tatgtctacc ttttctgcct atccgagact ttcttttcat
      241 atatgggata taaatgatta gatacagtac agcaaaaaat aaagcaaata cgcaagcttc
      301 ccaactacct aacgccaatt caaccatcaa ggtagcaacc ccaacggatg ctgcatacca
      361 gataaaaatc agaaacgttt ctttttttct gaccatgcga aacatgataa atagactaat
      421 aagcaatagt ggcaacacaa atatccaatt gcttccttcc atcagccact tgagtatatc
      481 aagcagcgac tgtgcaacag cgactaattt tcccatagtg aacacacacc tttttttata
      541 attaaaaaat tcgccttttt attctactat gaatcttctg gcttggcttt catttctctt
      601 atgtaatcat tttcttttat acaacttaaa aaaaaattaa accattagca taatattttc
      661 tgaatctatt cacgaaagtc tttttctatc tttcgttttc cacgactcaa ctgatctaga
      721 gtcaaaaaac taaaacgaca gttgcgcaaa tgacgcacaa gctgccgctt taataacaaa
      781 tagcctttca ttctcagcag gccacttatt tccagttaac tttctgttct tagcttttta
      841 ctcaactgga taatatatgc acttaatgaa gtcccaattt ccgaatgttg aagtccatat
      901 tcaatatttg tcttcatata tcctaactta tcgcctacgt cgtatctttt tcctttaaac
      961 tctctcgcat agaccgcctc tgtctggtta agagtctgaa gtgcatccgt tagctgtatc
     1021 tcgtttcctg ctcctggttc ttgagtttct aatatctcaa atattttagg agtcagtaga
     1081 taacgtccga taatggctaa attacttggt gcttttccca tttcaggttt ttctacaaac
     1141 ccttttactc tgcaacgacc cttttcgcta tctgttttag gatcaataat tccgtattta
     1201 gatgtttcat tttctggtac acgcatgacg gcaacagcgg aagcttgtgt ttcttcataa
     1261 tccatcatca attgttgagt caatgggata tcatctttca tcaaatcatc ccccagcatg
     1321 acgacaaacg gttcatttcc tataaatgct tttgcttgaa gcacggcatc ccccagtcct
     1381 cttggatgag attgacggat gaagtgaaga ttgacttgag tcgtttcttc aactaaagcg
     1441 agtaattctg ttttttcctt ttcccgcaaa ttgttttcca attcgatatt tgcatcaaag
     1501 tgatcttcaa tgggtctttt ttcttttccc gttacgatca agatgtcttc gattcccgaa
     1561 tccaatgctt cttccacaat aaactggatc gttggtttat ccacgattgg aagcatttct
     1621 tttgccatgg cttttgttgc cggcaaaaaa cgtgttccta gcccagcggc aggaatgact
     1681 gcttttttga ccttcactt
//
LOCUS       DARDHA010000254         1682 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000254 DARDHA010000000
VERSION     DARDHA010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.260"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..733)
                     /locus_tag="SDA10_002804"
     CDS             complement(<1..733)
                     /locus_tag="SDA10_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4241661.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSS"
     gene            complement(744..1412)
                     /locus_tag="SDA10_002805"
     CDS             complement(744..1412)
                     /locus_tag="SDA10_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEG4241662.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1542..>1682)
                     /locus_tag="SDA10_002806"
     CDS             complement(1542..>1682)
                     /locus_tag="SDA10_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG4241663.1"
                     /translation="EIHFYIYSFYATFYDILKHSYSKEKTMLLTRRLYQFSVPGWIEL
                     LS"
BASE COUNT          615 a          275 c          223 g          569 t
ORIGIN      
        1 tcgaactatt aaaaatgtca aatttcctta atgcgttatt gttttcagtt tttaacaact
       61 gaatagttgt agtttttaca gttctatttt taacaaatag aacttgatca attaattctt
      121 tcaaatagta aaaacgatgt tcagctataa caatagtaaa tcctttattt tttaaatcac
      181 aaagtaagtc agcaagtttc ttagtcgctt tgaagtccaa atttgaagac ggttcatcca
      241 atataattaa tttagagttt agcgataaac tacatccaat agaaattttc tgccgttcac
      301 cacttgatag cgaaaaaatg tttttatcta atatatttga aagatctaat aattcagcta
      361 attcattgat tcttttatcc atatgctctt ttgtaaatcc aaagttttcc atcgaaaata
      421 ctaactcact cgttgtattt gttgtaaaga attgtcctct aggatcttga cttacgtagc
      481 caatattttt tgatatactt tcttgtgtta aggattgtag cgagttattt tcaaataaaa
      541 tttccccaac caattttccg tcataaagtc ttggaataag tccattcatt aactttaata
      601 gtgtggattt cccactccca gaagaaccag taatcaatgt aagactgcct ttttttattg
      661 aaaaattaag gtcaacaagc gcttgttcct taacatcctt atatgaaaaa gaaacattgt
      721 taaattcaaa caattctttc acctcaaatc aaaatactta agaaaaaaat agtaacggaa
      781 aacaggatga tatagtctaa taagctcatg tttgttttat agtaagaact tcttttcaca
      841 ggagattcta tcccctttgt tattgcatta gcggtcattt cttcagcaat catagttgca
      901 cgtataagaa cgggaactaa cagaaattcc aaatattgca gaggctgaaa aaaagacaag
      961 cgaatgtttc tcatttttaa agattcttta atcattctta tttcagaacg ataagtcgga
     1021 aagaatctga tcactactgt aatacttaac attatttttt tcggtaaatg tattttttct
     1081 aaagaggata ataattcatt gctagtatag acactaacca acaaccatga gatcattacg
     1141 accggcaaca ttcgaaatac aatatataag gatgtcgcaa aaaatatagt aaaaaactct
     1201 atcttgatag catcaataat tttcattact aaaaacagaa ctgaataaat caagatgaaa
     1261 taaatacatg tctttatttt acgaaacatc aatactaaaa ctgtagcaat aatgaaacac
     1321 cccacttgaa atatatttcc ctttcctaaa aaaataatga ccggaaaaaa aatatttaaa
     1381 gtaatcaagg ttaacggatt gatttttgac actctttcac ctccaaaaaa taagatcgca
     1441 ctgttatctt aagctgataa aaatgcaccg ttattcagat aacagtgcga tcttatcaaa
     1501 aagcataact gagaacaatt ctcaatgcaa gcttttttta attaactcag taattcaatc
     1561 cacccaggaa cagaaaattg atacaatctt ctggttaata gcatagtctt ttcttttgaa
     1621 tagctatgct tcaatatatc atagaatgta gcgtagaatg agtaaatgta gaagtggatt
     1681 tc
//
LOCUS       DARDHA010000255         1663 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000255 DARDHA010000000
VERSION     DARDHA010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.261"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..451
                     /locus_tag="SDA10_002807"
                     /pseudo
     CDS             1..451
                     /locus_tag="SDA10_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            606..1181
                     /locus_tag="SDA10_002808"
     CDS             606..1181
                     /locus_tag="SDA10_002808"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEG4241664.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1224..1391)
                     /locus_tag="SDA10_002809"
     CDS             complement(1224..1391)
                     /locus_tag="SDA10_002809"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241665.1"
                     /translation="MENTFELYNKTTVDKLIPILVPTDLNGHDVLLRFNGFLGEDYRY
                     LKISDPQPLTE"
     gene            1408..1590
                     /locus_tag="SDA10_002810"
                     /pseudo
     CDS             1408..1590
                     /locus_tag="SDA10_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          586 a          260 c          330 g          487 t
ORIGIN      
        1 tggaaaaagc tgtcaatacg cttctcaaaa cggagttaac ggctttcctc gattacgaaa
       61 agtacgatcg cattggtttt aacacgggta attctcgtaa cggctcctat gaccgtacgg
      121 tcaagaccga gtatggggaa cttcatctcc agattccgcg cgaccgcaat ggcgagttca
      181 agcaacagac tgttcctgct tatagacgga cgaatgacac attagaggag accgtcattc
      241 acctcttccg aaaaggtatt accatgtcgg aaatcgcaga cttgattgag aaaatgtatg
      301 ggcatcacta cacgccccaa accatgtcca acatgactaa agtgctgact gaagaagtaa
      361 atgcatttaa agttagaact ctaaatgata agtatgtcgc tattttcatg attccttcag
      421 tttttcagat gatataccac tcaaaaaaca atatgagaga tgttcaggaa taccatctct
      481 aatggcttat aaattggttt tagggatagt agatgttata gacatatcaa ggaataaacg
      541 agcagctgca taaaagcaga tggaattaag gaataattga attgtaaaag aagggaataa
      601 atttaatggc acaattattt tacagatatg gaacaatgaa tagtggaaag agtattgagc
      661 tactgaaagt ggctcataat tatgaagaac aagggaagag agttatcata atgacaagcg
      721 caatagatac tcgatcagga tttggaaaag ttgagagtcg tataggtgtt tcgaaaccag
      781 ctattgcaat tacttctgaa atggatgtgt acggagaatt agtggagatt attgatcagt
      841 acggaaaagt tgattgtatt ttggtcgatg agtctcaatt tttaagtaga gagaatatta
      901 ttgatctatc acttatcgta atgaatgagg aagtaccagt aatttgctat ggattgaaga
      961 atgattttca aaataatctc ttcaagggat ccgaagcatt attgacattt gctgataaaa
     1021 ttgaggaaat taaaactatt tgccattttt gcaataaaaa ggctgtaatg aatgctcgta
     1081 ttgttgatgg tattccagtt tattcaggtg aacagattgt agtaggaggt aatgaagatt
     1141 acctttcggt atgtcgcaac cactactaca acgaagattg aaataaaaaa cgtgtaatca
     1201 aataattgat tacacgtttt ttattattct gttaaaggtt gaggatcaga aatttttagg
     1261 tatctataat cctcacctaa aaatccgtta aaacgaagta aaacatcatg accatttaaa
     1321 tcagttggaa ctaaaattgg aattaattta tcaacagtag ttttgttgta aagttcaaaa
     1381 gtgttctcca ttccttctgg ttctacaatc gaatctttca ataaacaaat caagaaatac
     1441 agccgcagaa aagagcagtt tcaaaatgaa gaatcactag agcgtttcct agtatccatc
     1501 tttgatacat acaatcaaaa attcttaaat agaagccata aaggcttcca acaagtgacg
     1561 gatacattag cttcaatgtt tactgagtaa cccattattt tgcagaagga cgagttattt
     1621 acacaaaatt attgacgctc cctgtttgga gtggtaaaaa gat
//
LOCUS       DARDHA010000256         1655 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000256 DARDHA010000000
VERSION     DARDHA010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.262"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            208..1377
                     /locus_tag="SDA10_002811"
     CDS             208..1377
                     /locus_tag="SDA10_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG4241666.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          608 a          236 c          263 g          548 t
ORIGIN      
        1 gaaagtttaa taagagtttg gacctagcaa acaatattaa tgaggtcatt aaatcttatt
       61 atctttagtg atataacgaa atatctacac agacaaaatg atagcgtaaa atattttgtg
      121 taaatagata tttagggtag aaaaaaagaa agtccattct gtaaaattaa agttaccaca
      181 accgaaattt tataggagga ctttctcatg cccaatttta ctacagaaat tatggaaaca
      241 ctaatcaata aaggtgattt agatgaatta tttcgatgtc atttagaatt agctgttaat
      301 tcattattgc aggctgaatt aacagctttt cttgattatg aaaaatatga tcgtgctggg
      361 ttcaattcag gtaattcccg taatgggaat tattcacgtt cattcagaac agagtatgga
      421 gaattaaatt tggtaattcc tagagataga aatggtaagt tttctcaaca aactttacct
      481 gcttataaaa gaaccaatga ctctttagaa accactatta ttcagctatt tcaaaaagga
      541 attactatgt ctgaaatctc tgaattgatt gaaaaaatgt acgggcatca ctatacgcca
      601 caaaccattt caaatatgac taaaatagtt tctgaagata ttattgcctt taaagaaagg
      661 tccttagaat ccagatactc tgtcattttt atggatgcta ctcacattcc tttaaaaaga
      721 caaactgttt caaaagaagc agtttatatt gtaattggca ttcgattaga tggaaccaaa
      781 gaggttcttg ggtttagtct tgctccaact gaatctgctt atgtttggaa agagattctt
      841 caagacctaa aagaccgtgg tttaaaagag gttttattag tcgtaactga tggtttaagt
      901 ggcattaacg atagtattca tagtgtttat ccaaatgctc aatttcaaca atgttgtgtc
      961 catatatcta gaaatatcgc tcataaggtt cgtgttagtg atcgacaaga aatctgtagc
     1021 gatttcaaat tagtttatca agcaagttca aaagaagaag caaataatca aataaggttt
     1081 atgatagata aatggaaaaa gcagtatcca cgagtggtta aattgctcat gaatcctgct
     1141 atattaacct tttataattt ccctccgtca atcagaagaa ctatttattc aactaatttg
     1201 attgaaggtt ttaacaaaca attaaagaaa tataccaaaa gaaaagagca atttcctaat
     1261 gaagaatctc tggagagatt tctagtatct caattcaaca actataatca aaagtttcta
     1321 tgccgagttc ataaaggttt taaagaaata catgatacat tagaatcaat gttttaactt
     1381 gatcaacaga atgggctttt ccatttacac aaaattattg acgctcccga caaaattatg
     1441 atcttgcgta aagttataaa atattaataa aaatatttta ttaatgaaaa gcttggtagc
     1501 acggttattc atatgctaat ttatttcact ttatagtaag gggagttctt agtatattgt
     1561 tcaaacttaa atttcatata atttaattat gtaaattagc tattctaaga acttctacgg
     1621 ataactgccc gtctaaaatt taaataagag cttag
//
LOCUS       DARDHA010000257         1648 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000257 DARDHA010000000
VERSION     DARDHA010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.263"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            138..1604
                     /locus_tag="SDA10_002812"
     CDS             138..1604
                     /locus_tag="SDA10_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEG4241667.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          462 a          296 c          337 g          553 t
ORIGIN      
        1 tttttatgat attgccatta aaaattactt gcttttatag aaaattagtt tatatttctt
       61 aaataagttt tttagttaaa tttttcggta aagatgtata atattttctg aaaatttaaa
      121 gaattggggt atagattatg gaaaaacagc aagacaaggg cttcttcggg cagccgaagg
      181 gattgtcaac tttgttcttc actgagatgt gggaacgatt tagttattac ggtatgcgtg
      241 cattacttat ctattatatg tacgatacag ttgccaacgg gggacttgga ttaccacgag
      301 caacagcatt agctatcatg tcgatctacg gatctttgat ctacatgtct agtatcgtcg
      361 gcggatgggt atctgaccga gtgttaggag caaaacgcac tgtattcttc ggtggagttt
      421 tcattatgct tggccacatc gtcttggctt taccatttgg tgtaagtaca ttgtttgttt
      481 cgatggcatt gattattatc ggaaccgcct ttttgaaacc aaatgtttct ggcatggtcg
      541 gacatcttta ttcaaagact gacttgcgaa gagatgcagg attttcgatt ttttacatgg
      601 gtgtcaactt aggggcatta ttagcaccac taatcgttgg tacaatcggt caagaatata
      661 actatcattt agggttctct ttagctgcta ttgggatgtt ctttggatta ttacaataca
      721 tcatccaagg acgcaagaca ttagacggag tgggtacaac accgccgaat cctttgacaa
      781 ctgaagaacg taaaggattc atccgtaact tgatcatcgc acttgttgtt atcgctgcag
      841 tattcggcgg ggcacaacta actggtcatc tgacaattga cttttttgtc aatacaatca
      901 gtgtattggg gattatctta ccgttgtatt actttatcaa aatgctgact tcaaaagatg
      961 taactgctga agaaaaacca agagtatggg cttatatccc attattcctt gcagcaatca
     1021 ttttctggtc attacaagaa caaggttcat ctatcttagc tttatttgct agtgaacgta
     1081 cacaatctac gctatttggt gtaacgatcc aagcaagctg gtatcaatca ttgaatccaa
     1141 ttttcatctt tattctgaca ccagtattcg taacgatgtg gacgaaactt ggtgaacgtc
     1201 aaccatctac agtagtgaaa ttctctcttg gacttttatt tgcaggatta tcgtacttgc
     1261 tattgatgtt gccgggtatg ctttatggta caagtggaaa agtaagtcca ttgtggttgt
     1321 tgggaagttt cttcatcgtg gttattgctg aattgtgtct ttcaccagtt ggtttgtctg
     1381 tgacaacaaa attagcacca cgagcatttg aatcacaaac aatcgctatt tggttcttag
     1441 ctgatgcttc ttcacaagca atcaatgcac aaattgcacg cttctacaca ccgggaacag
     1501 aatctgctta cttcggtatt atcggtgcag tagcaatcgt cggtggtatc ttgctattta
     1561 ctgtgaaaga accaatcaaa aaactaatgg gctctattca ttaatcaatg aacataaaaa
     1621 caggaattgc ttatttgcag cagttcct
//
LOCUS       DARDHA010000258         1647 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000258 DARDHA010000000
VERSION     DARDHA010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.264"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(20..91)
                     /locus_tag="SDA10_002813"
     tRNA            complement(20..91)
                     /locus_tag="SDA10_002813"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="SDA10_002814"
     tRNA            complement(113..200)
                     /locus_tag="SDA10_002814"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="SDA10_002815"
     tRNA            complement(225..298)
                     /locus_tag="SDA10_002815"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="SDA10_002816"
     tRNA            complement(312..382)
                     /locus_tag="SDA10_002816"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="SDA10_002817"
     tRNA            complement(384..459)
                     /locus_tag="SDA10_002817"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(478..551)
                     /locus_tag="SDA10_002818"
     tRNA            complement(478..551)
                     /locus_tag="SDA10_002818"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Met,seq:cat)
     gene            complement(564..653)
                     /locus_tag="SDA10_002819"
     tRNA            complement(564..653)
                     /locus_tag="SDA10_002819"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Ser,seq:tga)
     gene            complement(677..750)
                     /locus_tag="SDA10_002820"
     tRNA            complement(677..750)
                     /locus_tag="SDA10_002820"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Ile,seq:cat)
     gene            complement(764..837)
                     /locus_tag="SDA10_002821"
     tRNA            complement(764..837)
                     /locus_tag="SDA10_002821"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Met,seq:cat)
     gene            complement(865..937)
                     /locus_tag="SDA10_002822"
     tRNA            complement(865..937)
                     /locus_tag="SDA10_002822"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Ala,seq:tgc)
     gene            complement(957..1030)
                     /locus_tag="SDA10_002823"
     tRNA            complement(957..1030)
                     /locus_tag="SDA10_002823"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(994..996),aa:Pro,seq:tgg)
     gene            complement(1040..1113)
                     /locus_tag="SDA10_002824"
     tRNA            complement(1040..1113)
                     /locus_tag="SDA10_002824"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1077..1079),aa:Arg,seq:acg)
     gene            complement(1124..1209)
                     /locus_tag="SDA10_002825"
     tRNA            complement(1124..1209)
                     /locus_tag="SDA10_002825"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1173..1175),aa:Leu,seq:taa)
     gene            complement(1226..1297)
                     /locus_tag="SDA10_002826"
     tRNA            complement(1226..1297)
                     /locus_tag="SDA10_002826"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1263..1265),aa:Gly,seq:gcc)
     gene            complement(1309..1381)
                     /locus_tag="SDA10_002827"
     tRNA            complement(1309..1381)
                     /locus_tag="SDA10_002827"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1346..1348),aa:Thr,seq:tgt)
     gene            complement(1399..1480)
                     /locus_tag="SDA10_002828"
     tRNA            complement(1399..1480)
                     /locus_tag="SDA10_002828"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1444..1446),aa:Leu,seq:tag)
     gene            complement(1488..1560)
                     /locus_tag="SDA10_002829"
     tRNA            complement(1488..1560)
                     /locus_tag="SDA10_002829"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1525..1527),aa:Lys,seq:ttt)
     gene            complement(1564..1636)
                     /locus_tag="SDA10_002830"
     tRNA            complement(1564..1636)
                     /locus_tag="SDA10_002830"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1601..1603),aa:Val,seq:tac)
BASE COUNT          438 a          460 c          391 g          358 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
      481 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc
      541 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
      601 ccgcctgacg gttttcaaga ccgttccctt cagccggact tgggtaatcc tccatatgaa
      661 acttacaacg ccacaatgga ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
      721 gtctgctcta accaactgag ctaaaggtcc aggctcttaa aaatcgcggc ggaggggatc
      781 gaacccccga cctcccgggt atgaaccgga cgctctagcc agctgagcta caccgcgaaa
      841 aagatattca ttatttaaat gaaatggagc ctagcgggat cgaaccgctg acctcctgcg
      901 tgcaaagcag gcgctctccc agctgagcta aggccccgtg tttaaattta taattatcgg
      961 gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta ccaagctgag
     1021 ctacttcccg tttaattaac gcgcccaaga ggagtcgaac ccctaacctt ttgatccgta
     1081 gtcaaacact ctatccagtt gagctatggg cgcatatttg aaatgccgag gaccggaatc
     1141 gaaccggtac ggtgatcact caccgcagga ttttaagtcc tgtgcgtctg ccagttccgc
     1201 caccccggcg cgattgcttt ggcaaagcgg aaaacggggt tcgaacccgc gacccccacc
     1261 ttggcaaggt ggtgctctac cactgagcta tttccgcgtg tttaaagatg ccggctaaag
     1321 gacttgaacc ctcgaccctc tgattacaaa tcagatgctc taccaactga gctaagccgg
     1381 catgtaaaac ttaaattatg cgggtgaagg gacttgaacc cccacgccgt aaggcgctag
     1441 atcctaaatc tagtgcgtct gccaattccg ccacacccgc aaaaatatga gccgtacagg
     1501 gctcgaacct gtgaccctct gattaaaagt cagatgctct accaactgag ctaacggctc
     1561 atatggaggt taacgggatc gaaccgctga ccctctgctt gtaaggcaga tgctctccca
     1621 gctgagctaa acctccatgg ataactt
//
LOCUS       DARDHA010000259         1617 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000259 DARDHA010000000
VERSION     DARDHA010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.265"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            86..802
                     /locus_tag="SDA10_002831"
     CDS             86..802
                     /locus_tag="SDA10_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEG4241668.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            821..>1617
                     /locus_tag="SDA10_002832"
     CDS             821..>1617
                     /locus_tag="SDA10_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HEG4241669.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILN"
BASE COUNT          630 a          192 c          309 g          486 t
ORIGIN      
        1 gaggaagaaa cattatttca atgattaaga gtcgagagca caaacggtag acgatgttat
       61 ttttataaaa agatataatt tattaatggt cgataattat gaaaggaaaa atattcttaa
      121 aaaaaagagt ataggatgtc gtatgaaaat agtcgctata gtaccaatga agttaaataa
      181 caaaagattg ccacaaaaaa acacgaaagc ttttaccaat ggaaaaccat tgtgttatta
      241 tatactttca acacttttaa aagtaaaaca gattgataaa gtgtatgtct attgtagtaa
      301 cccagatatt aaagaattta taccagaagg tgtaacattt ttaaagagat ctagttcctt
      361 agatcaggat acaacgagta ttacacaggt attagccggt ttcacaaaag aagtagaggc
      421 tgatgtatat gtaatgacac acacaacagc accatttatt agcgaaaaat ctattgaaaa
      481 aggactagat gccgtgctga caggagaata tgattctgca tttgctgcaa aaaaattgca
      541 agatttctta tggaaagacg gtgaaccatt taattatgaa ttggacaata ttcctagaac
      601 acaggatttg ccgcctttat acgaagaaac tagtggattt tatatttata atcgcaatgt
      661 gatgaccgag ttgcatagaa gaattgggaa aaaaccttgc atagttgaag taggtgagat
      721 tgagtcagtt gatattgatg aagccgagga ttttatgatt gctgatgcga tttataatca
      781 tcttatttta caaggtagtt aagacagggg gaaggttagg atgaacaatg tacagatttt
      841 agattgcaca cttagagatg ggggctattg taacaattgg gaatttggat ttgataatac
      901 aaaaaaaatt atttctggac ttatagaatc aggaattgag attatagaat gtgggtttct
      961 cacaaataaa acagtataca ataaaaatgt tacaaaatat acgaccttag aagaattagc
     1021 acagataatt cctgaaaaaa gagaaggaaa attatttgta gcaatgatga actatggcga
     1081 atataatata gaggatcttc cagattatga tggttcatct attgatggaa ttaggattgc
     1141 gtttcataaa aaaaattgca atgaagcgtt gaaattatgt cgtgagataa agaaaaaagg
     1201 ctacttagtt tttgttcaag ctatggtatc tctcaattat acagatgaag agtttttagc
     1261 agtaataaaa aatgtgaatc agtttaaacc atacgctttt tatattgttg atagttttgg
     1321 aatgatgaaa ggaaaagatt taactcgtct tttttacatg attgagcata atctagaaaa
     1381 agatatacag ataggttttc actcacataa taacatgcag cttgcatatt ctaatgcaca
     1441 aaaacttata accgtacaaa caaatcgaag cctgattatt gattcttcgg tgtacggaat
     1501 gggaagagga gcaggtaatc ttaacacaga actatttgta gaatatttga atgataatgc
     1561 aggaaaaaaa tatcaattaa aaccattatt atatataatt gatgaaatac taaatag
//
LOCUS       DARDHA010000260         1575 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000260 DARDHA010000000
VERSION     DARDHA010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.266"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            20..1348
                     /locus_tag="SDA10_002833"
     CDS             20..1348
                     /locus_tag="SDA10_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241670.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            1359..1568
                     /locus_tag="SDA10_002834"
     CDS             1359..1568
                     /locus_tag="SDA10_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241671.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT          543 a          263 c          301 g          468 t
ORIGIN      
        1 atttgaaaga ggtgagaaaa tgattggcga agataaatta ttgatgacta tccagctcac
       61 agacttaaat gaagaagaaa aaaaacagga aataagcgat tgggctattt tgacacgtct
      121 gttgatccag ccaacattta tccgctcttt cacacaacaa gcggatccgt atgatcttcg
      181 aaagcttcaa gaaaaaataa tgctagcatc ggtaagagac gaaagttggt tagttgtagg
      241 aaatgacgag aacgaatgta gttttcgctt ggaagataat caattattga taaaaaatgt
      301 attgtctatc tctgttttca aagaaaagca atttttgatt cgagacttca ttcagaaaaa
      361 aatgattgct catggggtgt tcgcctatat gcgagcttat agcgagttta tctatcacaa
      421 tacaaaacag atctctcaac ggctattgtt tgagaaaaag gatgaaatcg aacatttacc
      481 taaaatgaaa caacaaaacg gggaagtagt agtcgattgc aaccaatttc caggatacga
      541 tcttttttac caaggtttat gtttcacttc gtgttgggaa atgtattata gtcgctatta
      601 tcatcaaatc atccctaaac cgatttttct cgacatccaa caagtagaaa aagtgaagga
      661 attagaaaac gaagtcatat gtatccagct ttatcgagac ccctttaact ggcaaaagcc
      721 aaacaatcaa atgtttcaat cctattttag ggaccagctt gggtttgatc atcttgcttg
      781 ggacaatggt gtcggtttat tgaaaccgcc attcgttgaa tacatttaca ctgaccatac
      841 gatccaatct gtccagtatc agaatgcaca aatgcaacct gttcctaaaa agaatgcttc
      901 attttttgta acaaaaagtt atgatatcca acaaggagat tataaagaaa ggcgtgttcg
      961 cggaacacta aatgctcagg cgtattttcc ttgggttgac gagaatcgat cacgcatgat
     1021 gtgctataaa gttatcgatc cgactgttgc tttggataat ggaatcgaag cttattgtta
     1081 ttatattagg gaatatctag aagtagaggt aacagatgaa aaatatcaag agtatctttt
     1141 atccttaaga atctatgttc cttcagaatc cctgtcagaa ttaccgttaa aggaaatcaa
     1201 acatagactc tctgatgtta ccttcaaacg aataaagaaa aagcggcgaa gtatccaatt
     1261 cgatgtcaag aaaggtgagc agcatctgcg tgtgcaattt ttagattacc gagaattaga
     1321 acaattgatt actttacaaa aaatatgagg tgaagaaaat ggataaacaa ctctattatc
     1381 gaataatggt tccggcatca ttattatccg tctgtctctt ttttgttcct aattttccta
     1441 ttcgatcagt catcggtttt ttggtcatgt ttggcgggat gggactttat tattatcttc
     1501 ttcataagga aagaaaacct gcttctgttt atatgaaaaa aacaagaagc gaaaataaaa
     1561 aacgataaaa cggct
//
LOCUS       DARDHA010000261         1539 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000261 DARDHA010000000
VERSION     DARDHA010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.267"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            9..305
                     /locus_tag="SDA10_002835"
     CDS             9..305
                     /locus_tag="SDA10_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241672.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            320..751
                     /locus_tag="SDA10_002836"
     CDS             320..751
                     /locus_tag="SDA10_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEG4241673.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            933..1238
                     /locus_tag="SDA10_002837"
     CDS             933..1238
                     /locus_tag="SDA10_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241674.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT          589 a          204 c          302 g          444 t
ORIGIN      
        1 gattttttat gaaaatcatt gacagttacg atcaacgaac catgcaatat ttgggctcgt
       61 ttgattatac agatgaaaat attattaatt atgttgcaag tttacctcct tatcaaagcg
      121 ttcgcttggt agattttgca agtgatgaat tggtattaac aacgatgggc aactttttag
      181 attatgtcgg agacaaaaaa tggttggaaa aaattttgtc taaattgatt gcgaaacaaa
      241 caggagaaga acccataaat gcgattcaat tgttcaatcg atatgaacaa gaaaacaatg
      301 attaaagagg agagaaaaaa tgaaaagatt aaaacgaagt atttttattg tgctagcatt
      361 gattgatttt gttgggacag gttgtgaagc aattgacgtt caaaaagctc ccttatcgga
      421 aataaaacaa gcaataacaa aacaagatga agaacaaccg ctaaatacac aagtagaaat
      481 agtgaatcaa aataaaccga gtttttctga agaagatttt tcgttagaaa atggaaccag
      541 gcaatttttt tcagatttgg atcgtttaaa tcgggtaggt gtagcaagtg cacttttgca
      601 taaaagcagg atgccaacag aagaacgaga ccgattatac attaagccaa ctggctggaa
      661 acaaaagaaa atgaagaatg gcgattatct ttataatcgt tgtcatttga ttggctatca
      721 acgtgcgaca agtttcccca taagtatgta atacgaaacg ggaggattaa tcttaagatt
      781 aatcacaact aacctatttg acagttggaa tgaaagctat catgtctgct gaatcaactg
      841 gtctgatact cctacatgca ataaagtcta ctaggaagtt attgtatgaa gctaggtaaa
      901 ggcggcagaa caatttctaa gtgtggaatc atatgaagtt ggatatgccg ggacgctata
      961 aattactcag ggtgagactg tttttaatta aactgacgaa cttgcgaatg tacgggtcta
     1021 taaccgactt atattcgaaa gatataagat tgcgaatgca aagaacatta tcgaaaagta
     1081 agaatgttgg tcatgaaatt cgagatggaa gactagcggt gtatctgtca agagttggta
     1141 aaacatgttt gattgattca gatgagagtg ggaatgcgac aaaacgtttt acagaagaaa
     1201 tagagcaact agcagaactg aatatacgat atttttaaat actttagcgg agaataaaaa
     1261 aatcctctac agaaaactac ggagggcaac atgtgctatt gtttcaacaa tacattttta
     1321 atttttacca taacaaaaaa gtacttgaaa aaaaacagaa atatgttaaa ttaaaaatga
     1381 aaaaatggaa gcggttactt attcttttga atctaagata aaaattcaaa agaataacat
     1441 aacccaaata tgattatact tgagattcta aatatttatc gaaaaggaat ttgttttaag
     1501 gaaaaatgat tctatggacg gttattgatt tatatttac
//
LOCUS       DARDHA010000262         1527 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000262 DARDHA010000000
VERSION     DARDHA010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.268"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            77..1246
                     /locus_tag="SDA10_002838"
     CDS             77..1246
                     /locus_tag="SDA10_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEG4241675.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
BASE COUNT          547 a          273 c          246 g          461 t
ORIGIN      
        1 aataaatgtt atcaagatac aagctatttt agctttattc tttctgtaaa aaagaaagga
       61 gactaaataa actattatga aaaaaggaaa acatgctaaa aaaagaaaat attcattttt
      121 aatgatcgtt gtcgtgttgt ttagccttag tgcgttctct acaggaacta ttcttggatg
      181 gcaattttac aataatgaac actctcagat agttgcccat gctcaatcag ctcaaaaaaa
      241 gttgatactg actaccaatg aacaacagct cgttgaaaaa atcgatcaac tattaagaaa
      301 aaatgattat attggctcaa tttacgtaaa gaaaaataat cgcatcatca tggaaaaagg
      361 ctatggctat gcgaataaac tcacgcaaca accaaatgat ccttctcttt attatcagat
      421 tggttcggtt caaaaagcat tgactactct tttgatttta aaacaagtcg agcgtgggca
      481 cctttcttta gatactaagc tggctcaatt ctatccacaa attgctggca gccagcaaat
      541 tactatccgt gacttgctgt atatgcgctc aggcttgcac cgaactgctt ctccaaccat
      601 accgatgact gatgaagaag tcgttcaatt tgcgattcag cacttaaaat ttacagatta
      661 tcagacctct cattatgatt cattgaactt taccatattg actggaattt tgatcaaatt
      721 aacaaatcaa ccgtatgaaa aattattgac cgatgatatc atcgaacctt taggattaca
      781 gcatacaaaa ttttatgacc aagtcaaaaa ttcatccaat catgcactat cctatcaaat
      841 gtctaaagag gatgattact ttaaaccact gacagaatct gaaacagaga tccataacga
      901 attagggact ggtaatatca gtatgtccgt ctatgattta aatacgctgt ttacacaagt
      961 tttatctgga aaactagttt ccaaagactt actcttctca ttatggcgtg aaaatgcgaa
     1021 tggacatcca tatagtggtg gggtatatag cggagataat tatatcctgt cgcaaggaaa
     1081 tatcgactct tttgatacat ctgctatatt tagcaaagat tcacaggatg ctatcatcat
     1141 ggaaagcaat ataaaagctg acaaaactat caaactgcct actgctgatc taagaaacca
     1201 aatctatgac ctgatggaag gaactactaa gttggcaaat agctgatatg atgaaaagat
     1261 agcaaatact acttctttga atagcatttg cttaaaaaaa gtactcgact aaacgtagga
     1321 aagagtgtgg gacaaatcta aaaatcaatt tttgctacag actttaattc caaataaaca
     1381 gaacagaaag aggtgttctt ctttctgttc tgtccaaatt attctaagat ttctctttac
     1441 caacattatt tgacaaaggg cctattaaat ctaaaatagt atctttagat tgctaaaata
     1501 tgtgctagca aatcattcat ggcttgt
//
LOCUS       DARDHA010000263         1510 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000263 DARDHA010000000
VERSION     DARDHA010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.269"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1368
                     /locus_tag="SDA10_002839"
     CDS             <1..1368
                     /locus_tag="SDA10_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG4241676.1"
                     /translation="DLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYP
                     DGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTD
                     VTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDL
                     NKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTV
                     TDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKK
                     GTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPD
                     GSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGS
                     KGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNR
                     KKENE"
BASE COUNT          600 a          228 c          325 g          357 t
ORIGIN      
        1 gatctaacag ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat
       61 gttactcccg gcggtacaat tgataccaac acaccaggta attacgaagg tgtcattgaa
      121 gtaacgtatc cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat
      181 cgttctgacg ccgataaata tgaaccaaca gtagaaggcg aaaaagtaga aattggaggc
      241 acagtagatt taacagataa tgttactaac ttgccagcgt taccagaagg aacaacagta
      301 acagatgtta ctcccggcgg tacaattgat accaacacac caggcaatta tgaaggtgtc
      361 attgaagtaa cgtatccaga cggtacgaaa gatacggtga aagttccagt agaagtgaca
      421 gacaatcgtt ctaatgcaga taaatacaat cctgaaggac aaaaagtgac tacagactta
      481 aataaagagc cagatgcatc tgagggaatt aagaacaaag aagatttacc aaaaggaact
      541 acttatgctt ggaaagagaa agtagatgtt agcacagctg gaaataaaaa aggtactatt
      601 gtagtgacat atccagatgg atcatctgat gaagttgaag tagacgttac agtgacagat
      661 actcgttccg atgcagataa atataatcct gaaggacaaa aagtgactac agacttaaat
      721 aaagagccag atgcatctga gggaattaag aacaaagaag atctaccaaa aggaactact
      781 tatacttgga aagaaaaagt agatgttagt acagctggaa acaaaaaagg tactgtagta
      841 gtaacatatc cagatggatc taaagaagaa gtagaagtta ctatttctgt agtagataaa
      901 aaagcaccga ataaacctca agttgatcct attacagagg gtgaccaaat tgttactggt
      961 aaaactgaac caaatgcaga ggtgacagtt acattaccag acggaagtca acaccatggg
     1021 acagctgata aaaatggtaa ttttacagtt aaagttccta aattagaggc agggacaaaa
     1081 gtgatagtaa ctgcaactga tgaatctggt aatactagtg aacctactaa tgtagttgtt
     1141 tcttccaaca aaaaagacag tggaaaaaac ggtagtaaag gtagtaagac tgacaatcaa
     1201 ggtagcaaca gcaatcaaga caaaaataga ggcaaaagcc agtcctcaaa agtttttcct
     1261 aagacgggag agagtgacag taatatcttt actattagcg gtggtttaat actgttagga
     1321 acgttaggat tattagggta taaaaatcga aaaaaagaga atgaatagaa aatcaatttc
     1381 ttaagtcttc tctgagaaga ggaagaatct ttttcttcct ctttttgctt ttttatgatg
     1441 ggaaataagt gttagttaaa gatgaatata ctttttatag aaaggaaaag aatgtaatta
     1501 agtggaaaaa
//
LOCUS       DARDHA010000264         1509 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000264 DARDHA010000000
VERSION     DARDHA010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.270"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            101..868
                     /locus_tag="SDA10_002840"
     CDS             101..868
                     /locus_tag="SDA10_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG4241677.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="SDA10_002841"
     CDS             1060..1458
                     /locus_tag="SDA10_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEG4241678.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          561 a          189 c          282 g          477 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatg
//
LOCUS       DARDHA010000265         1497 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000265 DARDHA010000000
VERSION     DARDHA010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.272"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..852)
                     /locus_tag="SDA10_002842"
     CDS             complement(<1..852)
                     /locus_tag="SDA10_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241679.1"
                     /translation="MIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIG
                     LTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRF
                     MIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEMNFQG
                     TYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEITFTF
                     KSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSYTFFS
                     KISLFISI"
     gene            complement(910..1497)
                     /locus_tag="SDA10_002843"
                     /pseudo
     CDS             complement(910..1497)
                     /locus_tag="SDA10_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          580 a          203 c          203 g          511 t
ORIGIN      
        1 tatagaaata aataatgaga ttttagaaaa aaatgtgtat gaatacctga ttgttacttt
       61 atttattcca ctttcacttt ttactgtaac aaaaggacct ccttgtttta gtactgtagc
      121 attttttgaa ttaacagtaa ccgttaatcc tttataccgg aatacaggta aaactattgt
      181 tttgcctttt gaagatttaa acgtaaatgt aatttcatct ttatttggag aaaagctact
      241 ataaataact tgaccatctt ctgtaacttg atggtttttc aaaagcaatt gatctttttt
      301 atagcctata ggtacataat caaaattctc aaattcttca agtttatttt ccatttgttc
      361 attagaataa gttccttgaa aattcatttc tttcaccatg ttaactactg atgaataatt
      421 taaaattgaa atagctaata ttaataatag tataaaattt cttctccttg tataagaaaa
      481 tttttccaac tgtggagaaa taaatagaga tactgaaaaa gatatgaaca atgttgaaaa
      541 tatcataaaa cgccaaggag cttgaataat atttaaagga gtattttgta aggcaaacca
      601 tggaaatatt tttgtacaaa gtagaataga tattataccc attatataag tgattttaga
      661 taagattcct aattttttaa agttaaatgt tagtataatt aaacaaataa gcaaagttaa
      721 gccaatacca aatgatagta attctgaatc tatacttcca atcaaaaact ctttacccag
      781 ataaattgct cttttttgta atatatccac ataaggtcta ttcagatctt gatacaatgt
      841 ctgctcaatc ataggaccta taaacgctag tgaaagaagt aaagtaacta tagcagcttt
      901 tattaattta ataattaaga agatttctat tttctttttc cgataaaatt gaataattaa
      961 agcaattata atcattacca tagtcatagc tacagaaaga acatgagtgt aaattaataa
     1021 agtcatgcta atcgccaaca taacccaaga ttcttttttt gagtcgtaaa gctttataaa
     1081 cccgagtaaa ataagaggaa gtattgacat tgcgagtaat tcccccattg cccctcgaag
     1141 aaatatttct acactcctaa aagaagatgt tgtatatata atagcgaaaa aggagctagt
     1201 aacatgtttt ttggtaattt catacattac ataatgacat ataaatagag taatgagcgt
     1261 aacaatataa agaaaaaggt tatagcctat tgctaaatta gtaaacttga tcaataaaaa
     1321 taaaggatat agagttaacc atgggtaaaa attatttact tgcatgccat atgatctaaa
     1381 cgtttcaaaa ttaatagggg agctaaaaat agattctaaa cttaacgcac gattaatgtg
     1441 aaatgccgta tcataaaagc caaatatttc catgttttta aatgctggaa ataaata
//
LOCUS       DARDHA010000266         1470 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000266 DARDHA010000000
VERSION     DARDHA010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.273"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          547 a          169 c          302 g          452 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg
//
LOCUS       DARDHA010000267         1467 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000267 DARDHA010000000
VERSION     DARDHA010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.274"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(61..279)
                     /locus_tag="SDA10_002844"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="SDA10_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="SDA10_002845"
     CDS             complement(545..739)
                     /locus_tag="SDA10_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241680.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(839..925)
                     /locus_tag="SDA10_002846"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="SDA10_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          514 a          230 c          194 g          529 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taagaactac ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca
     1441 atttggtagt atttttttat tatctta
//
LOCUS       DARDHA010000268         1460 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000268 DARDHA010000000
VERSION     DARDHA010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.275"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(141..1433)
                     /locus_tag="SDA10_002847"
     CDS             complement(141..1433)
                     /locus_tag="SDA10_002847"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEG4241681.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT          397 a          325 c          263 g          475 t
ORIGIN      
        1 ttcggaaaaa gggactgtga cacggttttt gtcacagtcc cttattctga ataaacagtg
       61 ttcaagttat tattatcgta tagataaaca cttcgactcg ctctggtaag aacgtcctga
      121 ttcagtcgtc gtttttatcc ttagatttgc gcccagacgc tttctaagat gtttgtttgt
      181 gttctatcag gtccgacaga aaatgttgag atacgtacac caacaagctc tgagatacga
      241 cgtacatagt tacgtgcgtt ttctggcagt tctgcaagtg ttttgcatcc tgtaatgtct
      301 tctgaccagc ctggcaattc ttcatagatt ggtttgcagc ggttcaactc tttcaagctt
      361 gctggatagt ggtagattag ttcgccatct aattcataag ccgtacaaat ttttactgtt
      421 tccaatccac ttaatacatc gatcgagttt aaagataggt tggtaatacc tgaaactcgt
      481 ttagaatggc gcataactac agaatcaaac cagcctacac gacgcggacg accagttgtc
      541 gtaccgtatt ctcgtcctac ttcgcggatt tgttctcctg tttcatcaaa taattcagtt
      601 ggaaatggtc catcaccgac acgtgacgta taggctttgc agacgccgac aactttattg
      661 atttttgaag gccctacacc gcttccgatt gttaccccac cggctactgg gttagaagaa
      721 gtaacaaaag gataagttcc ctggtcgata tccagcatta caccttgtgc gccttcaaac
      781 aacacgcgtt tgcctgcatc caaagcatca ttcaagataa cggaagtatc tgtgacgtat
      841 tgtttgattt gttgtccgta ttcataatat tcttcaaaaa tatcgtcaaa atcaagtggt
      901 tctgaatcaa acatttttac aaattgacgg tttttgtcat ctagattcaa acgaagacgt
      961 tcttcaaaaa tttctttatc taataagtct gcaatacgga tacctacacg agctgcttta
     1021 tccatatagg caggtccgat tcctttaatc gttgtaccaa tcttattttc gcccttagca
     1081 tcttcttgta attggtccaa tttgatatga taaggcaaga ttacgtgcgc gcggtcagaa
     1141 atacgcaaat tacttgtttc tacacctttt tcatgtaaat aagctaattc tttaaccaat
     1201 gatttaggat tgactacaac gccgtttccg atcacactga tcttgtcttt ataaaaaatc
     1261 ccagaaggaa tcaaatgcag tttataagtc acaccgttaa acttaattgt gtgccctgca
     1321 ttgtcgccac cttggtaccg tgcaatcact tcagcatttt cactgagaaa gtctgtgatt
     1381 tttcctttcc cttcatcgcc ccattgcgta cctactacta ctacagatga catgtgaaca
     1441 cctcgttcta tattatttaa
//
LOCUS       DARDHA010000269         1459 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000269 DARDHA010000000
VERSION     DARDHA010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.276"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..333)
                     /locus_tag="SDA10_002848"
     CDS             complement(<1..333)
                     /locus_tag="SDA10_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241682.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKK"
     gene            complement(335..1393)
                     /locus_tag="SDA10_002849"
     CDS             complement(335..1393)
                     /locus_tag="SDA10_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEG4241683.1"
                     /translation="MVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPGLLARQGF
                     IEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSS
                     SRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAK
                     DFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVI
                     RPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAF
                     ICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISI
                     VFWYYLGSISYLYLKKEN"
BASE COUNT          564 a          238 c          178 g          479 t
ORIGIN      
        1 ttttttgtaa acattggaga aaagtatttt tgtgaaataa ttttccccct gtataaaaat
       61 ttcatcataa acggataaat catccgagaa aaattgatta tggttagtta acaagttaat
      121 tttacgttta aaggaagaac cgattttccg gtgattcaca aaaacaacat cattaaatag
      181 ccctaattct tttatacgct cactatatct tctagcgttt tcaaattcag aataaataaa
      241 taaatctcct tttgattctc ggtaaaaatt ttgataaaca tttaatatat taatcaaatg
      301 taaaggagtc caagcaatga aagctgtctt catattaatt ctcctttttt aaatacaagt
      361 aagaaatgct tcccaaataa taccagaaaa caattgaaat gacatcgcgc ttattgaaaa
      421 gtacatgagc ctccaccatt tcatctataa aaatagatag tataaaaatt aaaacaatac
      481 caagaaacgc ataattcact ttacttctaa aaaatgtttt gagtaaacta aagatattta
      541 acacatagaa agcatagata catataaaag caattatacc acttgataca agtacttgaa
      601 gaaatccatt atgcatgttt ccatgtgctc tttttagttc attcacatct gtagtatcaa
      661 gatctacttt tttcaaaaat tttgcatcgc tattcccata gaaatcggtc tctccataac
      721 caaataacaa agttggtctt ataactttaa gcgcagcttt ccaaatcgta aaacgaccag
      781 ctgaaatttc ggagttatca ctagaatgct gtaatattac ttctttttct ggaagtggag
      841 ttttatctga ttcatttgac tctataactt ctacttttct atcaatcgat acaggaattg
      901 ttgctaaaaa atccttagca taattagtac caaaagaaaa atataccaat agaactaata
      961 atatgcttac catccatttg atttttacag gtagtacttc tctaatgctc tttctaaatt
     1021 ttgaattggc aaacaacaaa agaaataata ctaaaaaaga acaaattact acctgggcac
     1081 ctctagacga agaaagtgta atataaaata tttgtacgat aaaattcatc atataaaaaa
     1141 cacgaatcgt ttttgttatt tttgaaatta atacactaat taaacttaaa ataatagaag
     1201 tatatccaaa catagctcca acatttggac ttgtatagac accaaataac cgatgttcaa
     1261 taaaaccttg tcttgcaagt aatcctggcg ttcctgttgg gacatgataa gaaatcaaaa
     1321 aaataaattg atagatagat attagcgcag ctaaaaaaat tatgataatg aaaacattat
     1381 taaaatacac catttttttc tttttttcct cttcactttg tgaaaagtct gcaggaaaaa
     1441 ttactatcaa agtaatcag
//
LOCUS       DARDHA010000270         1453 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000270 DARDHA010000000
VERSION     DARDHA010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.277"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(28..1413)
                     /locus_tag="SDA10_002850"
     CDS             complement(28..1413)
                     /locus_tag="SDA10_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4241684.1"
                     /translation="MPKRTIKELIRKINVSLKQFLLIDEFIYSNHKGEIQTKETDQTK
                     KLAVFSKLKLCYLKESNRFNYLMLLINYPQTAVPKKYLLEQLYISPSYLEKLTKQLNH
                     SLKKFHLRILSVKNCCTLEGNELSIRLYLYFFLSDTFGGNDWPFSSLELNALKMNSTD
                     EQIEKISKQEKNIYLLLTLFILRNKQQAYFPKTSSEITELMDLLQHTHDYSEYFTSFS
                     VSRPCEETETERAYFNYFFYCFFPNQISFRQKQEIGFLFSTSKNLICRQVGQLMTYLT
                     QQFPLNHLENKIPIYFYFLVLAVVFFELLGESSSSFSTLCFPEATSNHSSSFIQEKKL
                     REKIDTIFSKNGERNQISQYLSQLIQHLLQTETKEQLLIHLQITKQFTGNYHLQSHLQ
                     QLFRKDAVKITAYSEHADLIITDQHEKISDETETFYLDTTHNTVLFGKLIKAVHELYM
                     KKGYENKTKRM"
BASE COUNT          465 a          197 c          260 g          531 t
ORIGIN      
        1 gaataagagg atgtgacaag aattttgtca catcctcttt gttttgttct cataaccttt
       61 tttcatatac agttcatgaa ccgcttttat taacttgcca aataacactg tattgtgcgt
      121 tgtatccaga tagaaagtct ccgtttcatc tgaaattttc tcatgctgat ctgtaatgat
      181 caaatcagca tgttctgaat atgcggtaat tttaacagca tctttgcgaa ataactgctg
      241 caaatggctt tgaagatgat aatttcctgt aaactgtttg gttatctgca aatggatgag
      301 taactgctct ttcgtttctg tttgtagtaa gtgttggatc aattgactaa ggtattgact
      361 gatttggttt ctttccccat tttttgaaaa aattgtatcg attttttctc ttaatttctt
      421 ttcctgtata aaagaactag aatgattgga cgtggcttct ggaaagcaaa gtgtagaaaa
      481 actactgctg gattcaccca acagttcgaa aaaaacaact gccaacacaa gaaaataaaa
      541 ataaattggt attttatttt ctaaatggtt gagaggaaac tgttgagtta agtaagtcat
      601 caattgaccg acttgtcgac aaataaggtt tttgcttgtg gaaaataaaa aaccaatctc
      661 ctgtttttgt cgaaaagaga tttgatttgg aaaaaaacaa tagaaaaaat aattaaagta
      721 ggctctctct gtttctgtct cttcacaagg gcgactcact gaaaaagaag taaagtattc
      781 tgaataatca tgtgtatgct gcaataagtc catcaattca gtgatttcag aagaagtttt
      841 tggaaaatat gcctgttgtt tgtttcgtaa tatgaataaa gttaacagca agtagatatt
      901 tttttcttgt tttgaaattt tttctatttg ctcatctgta ctgttcattt ttagtgcatt
      961 taattcaagt gaggaaaatg gccaatcgtt acctccaaat gtatctgaaa gaaaaaagta
     1021 aagatagaga cggatagata actcatttcc ttcaagagta cagcaatttt ttacagatag
     1081 gatccgtaaa tggaattttt ttaaggaatg attgagctgt tttgtcagtt tttcgagata
     1141 ggagggcgat atataaagtt gttcaagtaa atattttttt ggtacagcag tttgtggata
     1201 attgattagt aacatcaaat agttaaaacg attggattct tttaggtagc ataattttaa
     1261 cttagaaaaa acagctaact ttttagtttg atcggtttct ttggtttgga tctctccttt
     1321 atgattagaa taaataaatt catcaatcaa taaaaattgc ttgagtgata catttatttt
     1381 tcggatcagt tctttgattg tccgttttgg aatatgtaag tcattggata attcttgtaa
     1441 agaacaatta ttt
//
LOCUS       DARDHA010000271         1451 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000271 DARDHA010000000
VERSION     DARDHA010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.278"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..625)
                     /locus_tag="SDA10_002851"
     CDS             complement(<1..625)
                     /locus_tag="SDA10_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEG4241685.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="SDA10_002852"
     CDS             complement(784..1050)
                     /locus_tag="SDA10_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4241686.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="SDA10_002853"
     CDS             complement(1040..1396)
                     /locus_tag="SDA10_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG4241687.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          405 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg a
//
LOCUS       DARDHA010000272         1444 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000272 DARDHA010000000
VERSION     DARDHA010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.279"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(253..1278)
                     /locus_tag="SDA10_002854"
     CDS             complement(253..1278)
                     /locus_tag="SDA10_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG4241688.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
BASE COUNT          431 a          235 c          280 g          498 t
ORIGIN      
        1 aaataagggt aaatgtgttt aaaaaaacga actataaaaa cttatacaca acgtggttaa
       61 aaatagtttt ttcttccctg tggaaaaaat agtttaacac aaacattata gttatccaca
      121 gctatctaaa acagatggaa aataagaaaa attcgctaaa ataccgggag ggggtaacga
      181 gggatgagtg attgaaaaaa gaacagagta actgacttat cctcagtgtt tctgttcttt
      241 tataattctg ttctacattt cttgttgttg gatcgaatct gtgagagtaa tctcggttga
      301 aagaatgatt cttttttctt cagggtattg gtggtggatt agttggaata aaagttctgt
      361 ggctttgatt cctaactgat ttgtcggaat gtctatccga gtcaactgtg gcgaaaatag
      421 ggaggacaat aagggatcac tgaatcctgc gatcgaaaga tcatcaggta tctgctttcc
      481 agattgttct gccactttgt aaatactcaa tgattttaat gcatcggtag aaaaaatcgc
      541 agttggtctt tctcgtagct tcattaaatc attcattgct gccatgctat cttctaagcg
      601 aaagccgccg tctataatcc attcattacg gatatctagg tggtgatcaa ataagcattg
      661 acggtaacca ttgagacgat cattggatac ataaacttct gtcggagcat ggagacagcc
      721 gatatgacgg tgtccattct tgatcagtgt ttccaccaaa tcgtaactat cctggaaatt
      781 atccgtgtcc actgagtaga tgttcgtgta gtcgtttccg acttttccga ttaccacaat
      841 aggaatatta tattgatcca attcaaaaag aagtgtttca tccatcgtag agcttaaaat
      901 aataattcct cggatcattt tttcttggat ctttttcgcg catttttcta gctcttcttc
      961 tggtgttttt gccgtttgta gaatcaagtc gaagttattt tgttcagaaa cggcagaaat
     1021 ggattgaata acatctgaaa aaaaaggatt gccataggtc gtatgagaag tacgatgaga
     1081 taatgctaga atcgcgtcaa aaccatttcc tgacagtcct cttgcaattt tgctcggttc
     1141 ataattcaat tctttgattg cttgaagtac acttgctttt gctttatcag agatatttgg
     1201 ctggttattt aatactctgg agacagttga ttttgagaca ttagcttttt tcgcaatgtc
     1261 ataaatagta ggtttcaatt ttatatgcac ctcctttttg ggagcgtttt agaataattc
     1321 catagtacaa tagacattct tttttgtcta tgattttgaa aatttaggaa tagcttgcat
     1381 gatcatgaga gaccatattt ttttaagaag ctattaatta ttgatacata agctttctta
     1441 tttg
//
LOCUS       DARDHA010000273         1440 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000273 DARDHA010000000
VERSION     DARDHA010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.280"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            105..1406
                     /locus_tag="SDA10_002855"
     CDS             105..1406
                     /locus_tag="SDA10_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG4241689.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          474 a          288 c          264 g          414 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DARDHA010000274         1426 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000274 DARDHA010000000
VERSION     DARDHA010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.281"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(270..758)
                     /locus_tag="SDA10_002856"
     CDS             complement(270..758)
                     /locus_tag="SDA10_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG4241690.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(780..1382)
                     /locus_tag="SDA10_002857"
     CDS             complement(780..1382)
                     /locus_tag="SDA10_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG4241691.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT          459 a          324 c          205 g          438 t
ORIGIN      
        1 ttaaagggga aataaaccaa ttttacagaa tatattcttg taaaaaaaaa tacagaaaga
       61 agcataagct tctttctgca tttttacggt acttgattac tcaagcagac aagcccttct
      121 ccacaaaagt cttgcccgac acccctgcat ccggcaggtg ataaagatag aaatcaatta
      181 tcctgtgcct ccaaaatcaa ttaggagctc tgtaaaccag agtccttctc gacccgtcgc
      241 ataaatttac tttaacacag ttcctatgtt tatgcgagag cttttttcat tttataatcg
      301 accatttgaa atccttgacc gatatctgaa ataagttctt ctgtaatctc aaatccttca
      361 tgcttataaa cctcgattgc ttgatgattc ccctgattca ctcgcaaaaa gatctctctt
      421 tcctttgttt gcgcagcaag ttcttcatac cagcgaaaaa ctgaagaagt caatccttga
      481 ccacgtactt tatttagcaa atataattta cttaaataaa tttttccatt tatctcttca
      541 taagcagtgt aaccgacagt cttttcttcg ctttttaata aaaagtagga cacaccttct
      601 gtcagttctt tttgaatctg tttttttgac tgataagttt tcagcatgta atccacttgt
      661 ttttgaccaa tgatcggcac aaaaacttcc ggccatactt cttgtatgac tgccatcagc
      721 tcatccagtt cattttctgt tacttttatt ttttccatac acccacgtcc ttttttacat
      781 tataaatgac cttgcgcttg atggaataaa aagagcatga tcatgctata cacacccacc
      841 gaccacggtt tcaagaaaat cacaatctgc ataaattccc taaaagataa tttcgtcaaa
      901 ccagctacta gacagatcaa gtcgtctggc gcaaagggaa gcagcaaggt aacaactagt
      961 acttttttaa tatctttcgt ttttcttgtc agcaattcat caaatttctg atactttttt
     1021 ggcgataaaa taagctggac aaaggctacg ccatagtaac ggacaagcaa aaatccgatt
     1081 gcttccccaa tcactaggcc aatacaacta tataaaagcc caaatccatt gccaaacatc
     1141 agcatgcccg ctactgagga gatccctccc ggcaaaatag gaatgatcac ttggatcact
     1201 tgcaacagaa caaatatcat cactgcatac ttgccaaatt gctggataaa cgcttgcaaa
     1261 gacgtgctcg atcgaaaaat cccatgatga taagcataaa gaatcaacac aaagaaaaaa
     1321 aacagaccaa taatgggcat gatctgaaga catttttgta aaactgatcc ttttttagac
     1381 atctatattc cccacatctt ttttcattta ttaaaactgc ctcctt
//
LOCUS       DARDHA010000275         1417 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000275 DARDHA010000000
VERSION     DARDHA010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.282"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(150..1337)
                     /locus_tag="SDA10_002858"
     CDS             complement(150..1337)
                     /locus_tag="SDA10_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HEG4241692.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DARDHA010000276         1414 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000276 DARDHA010000000
VERSION     DARDHA010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.283"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(16..1047)
                     /locus_tag="SDA10_002859"
     CDS             complement(16..1047)
                     /locus_tag="SDA10_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG4241693.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          424 a          232 c          243 g          515 t
ORIGIN      
        1 gatcatacat ttgttttata tggaagaaac ccgaacgaat gccgctaatt cgtgataaga
       61 aaaatcaacc atcttttccc agatatcttg aaaagaatgg gatgagaggt atcgattacc
      121 acataaataa tttaccgcgt cagcatcatc taaatatagt tctgcataat aacgatatgt
      181 ttttctgcat agttctggcg atacgttttt atatactgta attttgctca aaatttggcg
      241 aatagtccga ggagaaattt gctgatcatg tgaggaacga aaaacaaaag aattttcgtc
      301 cgtattttgt gctgttaact taaaatagtt ctccagaaat ggcatcgctt cttttggtat
      361 tggtacttta ttcagtctgt tttcttttct attcagatta agaatctgct tggtcaaaga
      421 tacatcttta cgtttaagtt ctactacttc atggatcttt aatccgcaat acagaagaat
      481 ggcagtaatt gcaaggttcc tcggtctatt cttttcttca aagctagctt ctctgcttgt
      541 ttgaaagcct tcaatcggga taaacaaatg ctcaaaatct ttgaaaagtt ctgcatcgat
      601 atatcgcgga gctgctttgg ttgtttccgg taaacttgta cccaaaagag aaatctcttc
      661 ttcaatcagt cctttttgat acaaaaatcg taaaaaatga tttaatgaat attgcttcct
      721 tgaaatcccg taatagctat tttcaaacaa ttgctccgtt tcttggttcg ctgtagtttg
      781 gaacgaacga gtatgtcgat agattttagc taagaaattc tgataatctt gtttttctaa
      841 atggataatc agctgatcat ccacttcttc agaaaatact tttctcaaat aatcagtaaa
      901 cagatgaaaa tcttttgcgt attcatagcg actgcctaat gaaagggaag aacgattttc
      961 ttctaaaaac tgttgaaaga cggaagggag caagtgtgta cgttgcttta acttttcttg
     1021 cagttgtttt ctagttttgc tgtccattac aaattccctc caatagtgtt cattttctat
     1081 attttaccgc atttcatcaa atgaagatag acagtttgtc ttgatcttag gaagcaaatt
     1141 tttaactaag tactagctaa aagatatgaa gtaagaagat acatttttag ttattttatt
     1201 agtgcagtca ttcttttgat cttttaattg atttatcctc tttttagcta tgtggttact
     1261 tcatgagcaa tgaaatggat ttggttcgtt ctaatggcag gaggctcttt cttttatttc
     1321 cgaaaaatta gaaaacattt tttcagaatc gattggctct ccatttattc aataatggtt
     1381 tgtctaacga tattgttggg ataattctta aaga
//
LOCUS       DARDHA010000277         1394 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000277 DARDHA010000000
VERSION     DARDHA010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.284"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            476..727
                     /locus_tag="SDA10_002860"
     CDS             476..727
                     /locus_tag="SDA10_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241694.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            761..1039
                     /locus_tag="SDA10_002861"
     CDS             761..1039
                     /locus_tag="SDA10_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241695.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
BASE COUNT          495 a          192 c          262 g          445 t
ORIGIN      
        1 atacaaataa ttggtttatt ctggcaggcg taatgctttt ttctagtttc tatttattta
       61 gtaatgctct attagcacag cgagggaaat ataagtttaa aatattagat tagatattat
      121 ttttagaggg gataaatata aaatgaaaag aggttattta taatggaaaa agtattacca
      181 attaagagct actgtattcc aaggcttttc atcatagaat ttcaaaagta aagacatgtg
      241 tttcacaaat agtgtctatc ctggctaaat aattcttcca ctatctgctt taatttcagt
      301 agacttttcg gcaagttatt tgctaatgag atgaatagag attacaactc gcctataacc
      361 gcttaaaaca agctttttta aagtatagtt agcaagtcgt attcatatgt tcaaagagga
      421 gggcttatga actttgaatt cattccgtgt gaacagaaca gtagggggaa gtaaaatgaa
      481 agaaaagcct ataaataaat ggtcattgta taaaaattca atgtttggag ttgttatctt
      541 tggtgtttgt gctggattat tccttttcat attattacaa acggtgactc ttttctcttc
      601 taatcctact tatggatgga gagacagtat agccttaggg atgtttgcgg gaggatatgg
      661 attcttattg ttgttctcaa ttctcacact aagtccaatc catggactca ttcaactttg
      721 gaagtaagaa cgagccttaa acttttcgtt ctcggaagaa atggaaaaac acaacataac
      781 aggcagtatg tatgtggatg atcaatggtt tatcagcact gatgaggtaa aaatagctgc
      841 cattaggaga gattacctgc agaaaattca taaaataaaa gatcctaatg ggaaaaaggc
      901 aaggttaatt ataactttga taacatctaa tggtaagaag ttaaaacttg caggatcata
      961 tgataatgtt aaaaagttcg aacattggta tctgcaaaaa gacagcatta gtcttaataa
     1021 acaatttgaa aaaaattaat caaagtcatc ggcaacacga ataatttgac atcttgatat
     1081 tgtgatataa gcaaaaacgg catttctgag accgaacagc ataaaaaatt taaatgttgg
     1141 aatttatata agcaaaaccc aagaagctag atgcgataga gcatttaatt tcttggggtt
     1201 ttgcttggga atcagcatat caattaaaat gatcatctcg gtatttcaaa agtgggtacg
     1261 attacaaata gttggagcaa aatagtgggt aaaattacca ttccagtgtc tgtcttgcct
     1321 ctttttcgtc aatcacaaat agacgagatt cataggaaaa atcgatacag ggtatgattt
     1381 caatgatatt atga
//
LOCUS       DARDHA010000278         1388 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000278 DARDHA010000000
VERSION     DARDHA010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.285"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..787
                     /locus_tag="SDA10_002862"
     CDS             <1..787
                     /locus_tag="SDA10_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEG4241696.1"
                     /translation="VLSCATLLKEAGIPNIAIVTNGSLVTQDVAKELFKAGINRFQVS
                     LDGKNASSHEALRLYPNSFEKAINAIKLFRELSEEVDVAFCPTSFNYNQLREVYQLVN
                     TLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLVSDINYIKRNEDYTKIDWGDPIDH
                     IIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGNIKCHSLSKYWRAGLYKVFSLDLV
                     QDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILDEYKHDILI"
     gene            843..1061
                     /locus_tag="SDA10_002863"
     CDS             843..1061
                     /locus_tag="SDA10_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241697.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            1132..>1388
                     /locus_tag="SDA10_002864"
     CDS             1132..>1388
                     /locus_tag="SDA10_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241698.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTI"
BASE COUNT          520 a          170 c          262 g          436 t
ORIGIN      
        1 agttttatct tgtgccactt tattaaaaga ggcaggtatt cctaatattg ctattgttac
       61 taatggatca ttagtaactc aggatgttgc aaaagaatta ttcaaagcag gtattaatcg
      121 atttcaagta agtttagatg gaaaaaatgc tagtagccat gaagcactta gattatatcc
      181 aaacagtttt gaaaaggcaa ttaatgctat caaattattt agagaattgt ccgaagaagt
      241 ggatgttgct ttttgtccta ctagttttaa ttacaatcaa ttacgagaag tatatcaact
      301 agtaaatact cttaaagtga agtctttaag aattcaacca tttatgctta gtggtagggg
      361 aatgcaaaat agggaaatag tgccaactgt ttctcaatat agaaaactag tttctgatat
      421 taactatatt aaaagaaatg aagattatac caagatagat tggggagatc caattgatca
      481 tatcatccga cacagaactt tattgaaaga tttttcgaat tttgtttcta ttaggtcaga
      541 cggaggatta atggcatcac cttacttgcc aattcaagtt ggaaatatta aatgtcacag
      601 tctttctaaa tattggcgag caggacttta taaagttttt tctttagatt tagttcaaga
      661 tatagctact caagtttctt caatacaaga tatgagtaaa gaattaaaaa aaggacatat
      721 taaatggctt aatgaagata tagaaattga tattctcgat gaatacaaac atgatatttt
      781 aatttaggtt tatcattgga aagtttagat aaacaaagta cttttagaaa ggagggaaaa
      841 aaatgatttc tgaacctcaa atgaataata tgtatgaaag cccaactata atgggagctg
      901 gtgaagctgc gccaagagct tgttcagcaa ttatggtgtg tgtgttatta gcatctgcag
      961 gagtaggtat ccaagcagga gcggtgtata cttatgctgt agttgctaca gtagctgtag
     1021 ctgcagccgc agtttggaaa acagtaggat tagttggtta ggaattaata ggagactaac
     1081 tgtctcctat tgattccaat gattaagcta aggaggaatt ttaagaaaat aatgaaatac
     1141 aataatttaa aaccgaaatt acttaatgag aacatagaat atataagaga agaaaattat
     1201 ggatatttaa ccatagttcc caaaaatgga actgaattga aagataacgt tttcaatcca
     1261 acagtaagat atatattggc taactgtact ggaaaaaaca gtgtaaaaaa aatagcgaat
     1321 ggtttaaaaa agaagtttcc tagtttagaa tatgaaaaat tctatcaaga tgttgtagga
     1381 actattaa
//
LOCUS       DARDHA010000279         1375 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000279 DARDHA010000000
VERSION     DARDHA010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.286"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            191..445
                     /locus_tag="SDA10_002865"
     CDS             191..445
                     /locus_tag="SDA10_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG4241699.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="SDA10_002866"
     CDS             446..763
                     /locus_tag="SDA10_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG4241700.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          493 a          196 c          266 g          420 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atatt
//
LOCUS       DARDHA010000280         1372 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000280 DARDHA010000000
VERSION     DARDHA010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.287"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            122..>1372
                     /locus_tag="SDA10_002867"
     CDS             122..>1372
                     /locus_tag="SDA10_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG4241701.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          428 a          271 c          261 g          412 t
ORIGIN      
        1 gctctatact ttttgatgtt ggtgaacttc accaacatct tttttgtata aaaaaatata
       61 gaaaagacac cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa
      121 gatgtctcat actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt
      181 cgaggaaaat tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg
      241 aactcttctg tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat
      301 cattcgttgg gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac
      361 ttatctggag ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc
      421 ggatacctct gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat
      481 tgctcgctta aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac
      541 ttcttcggtc tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac
      601 tttgccaaag gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat
      661 gagttttatc atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg
      721 cttgccttat ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca
      781 attgattgtt attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa
      841 tgctcagttg atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa
      901 tcatcgagtg aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt
      961 agggaaaaaa ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta
     1021 tgaaaaacgg gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga
     1081 tcgactgtta gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct
     1141 ttatgcggta aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag
     1201 cggtctgcca gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat
     1261 ccaaaatgcg cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat
     1321 caaagtatta aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DARDHA010000281         1365 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000281 DARDHA010000000
VERSION     DARDHA010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.288"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1311
                     /locus_tag="SDA10_002868"
     CDS             <1..1311
                     /locus_tag="SDA10_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4241702.1"
                     /translation="TEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLL
                     TEIVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVL
                     FGVYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAY
                     GNQPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFE
                     LDGAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRK
                     NWIFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYL
                     FLLVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYL
                     SDCLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          477 a          201 c          245 g          442 t
ORIGIN      
        1 actgaattca aaacaaagcc agagatccaa atttcagaag aagcagagat ccatgtcgaa
       61 cgtctgaatt tgacggatat caaaaattta agaagaatct actttcaacg atcaaaaatt
      121 gctcgactgt taacggagat cgtggattca aaagtaacga tcgaaaaatt tgcagagaat
      181 cattttttaa gcatatcaag agcctatgta aaaagaagag aattagtctc ttttcttgaa
      241 cagcatcaaa taaagttaaa gaaaaacata ttagttgggg aagaaataaa catacggaac
      301 gtattatttg gggtatattt cggggtcttt aatggttttt ctatgccatt tagagaaagc
      361 gtcatgaatg aagcaaagag tatcgtgcaa tattttgaat atctgttcaa tttaaaactg
      421 tctgcaacaa aacgaatcaa attcaatttg ctaaccacta ttagtttgtt gagggcctat
      481 gggaatcaac caatcggaaa acgtttcttt tcagatgaat actttaagag ctcagaagga
      541 atgaagatca tcaaggattt atcacatatt tcttttcttc atatccctaa agagcagatc
      601 attgatgaga tttcttatat catgttgttt ttgatcacgg aagaagaatt tgaattagat
      661 ggcgcatttg gacttgcgga agctcatttt catgatatcc acatgattag tcaaaaaatg
      721 tcagtggaat tgattgatca attaccattt tcgattgaaa tctctgaaga agaaagacta
      781 tatatacgaa aagaacttcg tatgggtatg atgcatgtca atcgaaaaaa ttggatattc
      841 gactttgaga tgactagctt tacgtctaaa aagcagctac agtttttctt cgagacgtat
      901 ccaatattca gtacagcaat ccaaaaggtc atacaagaga ataaacgcta tttcttggga
      961 aaagatgaac gtttcatgat tcgcttattt tatgattatc tctttttatt ggtcggtatt
     1021 tgtccggtat catattttga agcaccgatt catgtctgta ttgatttttc gcaaggatct
     1081 gcttataccg attacattat ttctcaagtg aaaggattta aaaattacaa tattgtgatt
     1141 gagagtcgaa tcacttctcg tacgaatatt tatttatctg attgtttact tgaaaaattt
     1201 aataaagaac agataatttg gaaaaatcca ccgactccag atgactggga atattttgga
     1261 aataccatca tcagagaaaa aaatagacat ttaactgaaa aaatcagcta gtatttatgg
     1321 atggcagctt tgtctcacat atttattagc tgatttatgt ttttt
//
LOCUS       DARDHA010000282         1355 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000282 DARDHA010000000
VERSION     DARDHA010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.289"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1355
                     /locus_tag="SDA10_002869"
     rRNA            <1..1355
                     /locus_tag="SDA10_002869"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DARDHA010000283         1354 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000283 DARDHA010000000
VERSION     DARDHA010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.290"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            156..563
                     /locus_tag="SDA10_002870"
     CDS             156..563
                     /locus_tag="SDA10_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241703.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            577..978
                     /locus_tag="SDA10_002871"
     CDS             577..978
                     /locus_tag="SDA10_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241704.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            980..1351
                     /locus_tag="SDA10_002872"
     CDS             980..1351
                     /locus_tag="SDA10_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241705.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          526 a          171 c          234 g          423 t
ORIGIN      
        1 aaatgtcaga tatgcttaca aatatactta taaatatgct tacaaaatta gctaatattt
       61 ccgttgacaa agctttgtta ctattgttaa actaaattga ctagcaaaag tgaaacataa
      121 atttattaaa taaatgtaaa aattaggaga aatcaatgtc ttatagagta caattaataa
      181 tttcagaaga tgtagaaagt cagcaatttg gcactaatgt tattaataaa gtaattaatc
      241 cagctctttc tattaatgcg cctttaattc ctactgcttt atcattttct gtaacagctg
      301 tggtgtctga gatagaggat acagaaaaga taaaaatagt agaaattgaa gttttaaaca
      361 agaatgaaaa acaaattttt tctacgggtg aagtatcggt aagcttgcct cctcaagtta
      421 acgatataaa ctttaatata aacgccagaa atgtcttggt tgaagaagca ggagagcatt
      481 atgctgttgt taaatttaat ggaactgaga ttggaaggca aatctttgat atcaaggtca
      541 acaaaccagt ggaaaaaaat taaggagacg atacggatgc ttgatatagt accatcaaat
      601 acacatcgga gtggtagctt aataaatttt gctgcgattt ttgcttgcgc atcggtaatt
      661 ctagcatctc cagttgttgt ggatgcaagt tctactccga aaactaaaaa tgataatcaa
      721 cttgtaatta ctacaaaaaa tgagattaac acaatttcaa gtaatgatgg caaaattttt
      781 gatgtgataa atacggtttt aaaaagaaaa gctgaaatca atataaaata tgatgaagat
      841 ttaaacttgt atttctttgt tatcaaaaca acatctgaat tgttttcctc agattacgat
      901 gttttggata ctctcgataa tgtattatct gactataaat atatggggaa atctgtagta
      961 gcaactttgg gggaataaaa tgtttgactt taatgaatat ttatctattt ccaaacaaat
     1021 tgaatcagat gataaatata attctaaaga atcttataga agaactgctg tttcaagagc
     1081 atactacagt gcttttaaaa aatctgatga gtatttgaaa gaaaattatg atattattta
     1141 taatggaagt agcggaaaag gaagccacca aaccgtttgg aatttgtttt caacagttaa
     1201 ggaattgaat accttaggaa tacataatag tgggtataga atgttagaga aaagaaaatg
     1261 tgctgactat attcctaatg aaaccattac gaaaacagat atggcgctga tgaatcgaga
     1321 agcagaaaaa ataataaaca aactcaccta atga
//
LOCUS       DARDHA010000284         1311 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000284 DARDHA010000000
VERSION     DARDHA010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.292"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..830)
                     /locus_tag="SDA10_002873"
     CDS             complement(<1..830)
                     /locus_tag="SDA10_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HEG4241706.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
     gene            complement(901..1302)
                     /locus_tag="SDA10_002874"
     CDS             complement(901..1302)
                     /locus_tag="SDA10_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEG4241707.1"
                     /translation="MFAPNETHNLSDEFFKMFLKRYIDSNDDTKAAINYFDALLNSYE
                     DLIVYRENKNIDILLVSEKNKIVVCIENKILSSESGGQLNKYQIYIERNYSNYKKIFV
                     FLTPDGNEPTNPSWGIITYKNIVEESVKYFV"
BASE COUNT          433 a          230 c          181 g          467 t
ORIGIN      
        1 taaactaatt tgaaatcgct acagatttct tgtcgatcac taacacgaac cttatgagcg
       61 atatttctag atatatggac acaacattgt tgaaattgag catttggata aacactatga
      121 atactatcgt caataccact taaaccatct gttacgacta acaaaacctc ttctaaacca
      181 cggtctttta gatcttgaag aatctctttc caaacataag cagattcagt tggagctata
      241 gtaaatccta gaacctcttt agttccatcc aacctgatac caattactat atagacagct
      301 tcttttgata cggtttgtct ttttaaggga atatgagtag catccataaa aatgactgag
      361 tatttagctt ctaaagttct ttctttaaat gccgaaacat cttcagacac aattttactc
      421 atatttgaaa tagtttgtgg tttgtaataa tgaccataca ttttttcgat tagatcagag
      481 atttcggaca tagtaattcc tttttgaact agctgaataa tagtggtttc taaagagtca
      541 ttggttcttt tataagcagg taaagtttgt tgagaaaagt ctccattcct atctctaggt
      601 attactaaat tcaattcacc gtactctgtt ttaaaagagc gcgaataatt cccattacgg
      661 gaattacctg aattaaatcc agctctatca tacttttcat agtcaagaaa taccgttaat
      721 tcagcttgta ataaagcgtt aatagccaat tctaggtgac gacgaaacaa atcatccaaa
      781 tcacctttat tgattagtat ttccataatt tctgtagtaa aatcgttcat atggaagtcc
      841 tcttttctgt gaatgtgttg tggtaactct attctacaga agggacttcc ttttttctat
      901 ttacacaaaa tattttacac tctcttcaac gatattcttg taagtaatta tgccccaact
      961 aggatttgtg ggttcatttc catctggagt taaaaaaaca aaaatttttt tatagttaga
     1021 ataattgcgt tctatatata tttggtattt attaagttgc cctccagact ccgaagacag
     1081 aattttattt tcgatgcaca ccactatttt atttttttcc gataccaata aaatatcaat
     1141 attcttattt tctctgtaaa caattaaatc ttcatagcta ttaagtaaag catcaaaata
     1201 attgatagca gctttggtat catcattaga atctatatac cttttcaaaa acattttaaa
     1261 aaactcatca gataaattgt gggtttcatt tggagcaaac aaaaaagcta a
//
LOCUS       DARDHA010000285         1298 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000285 DARDHA010000000
VERSION     DARDHA010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.293"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..526
                     /locus_tag="SDA10_002875"
     CDS             <1..526
                     /locus_tag="SDA10_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4241708.1"
                     /translation="LMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSN
                     MYVFLVLTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQA
                     LAGGVGGWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKE
                     KVLENYNLLNQKHN"
     gene            537..>1298
                     /locus_tag="SDA10_002876"
     CDS             537..>1298
                     /locus_tag="SDA10_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG4241709.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYI"
BASE COUNT          460 a          144 c          255 g          439 t
ORIGIN      
        1 tttgatgttg gtacttgttt cacctgtaag tttaattttc agtaaaaaat acggtaaaaa
       61 agaattggcg tcagtaggga tgttcttctc tgctattgta tatttacttt tatttttgat
      121 aaagcctagt aatatgtatg tattcttagt tcttacttct gttggatata tggggttagg
      181 tatttttaat acagtcattt gggcgaatat tactgatgta atcgatgatc aagaagtgaa
      241 atctggccaa agagaagatg gaactattta tgcagtatat tcttttgccc gaaaaattgg
      301 acaagcttta gctggcggtg tcggaggttg gactctttct ataatagggt atgattcgtt
      361 agcaaaagta caaagtggag aagttttaca aaagctgtat aactcttcaa cgttgattcc
      421 agcagtttgt ttctttgttg taggattgat tttatttttc gtatatccat tatctaaaga
      481 aaaagtatta gaaaattata atttgctaaa tcaaaaacat aactaggaga aaaaacatga
      541 acagttggat attttgtatt gattcagatg gttgtgtcat ggatacaatg acttataaac
      601 acaatctctt tttcgggcca attgctgcag agatttttga tgttcatcat agggaaaaat
      661 tcctacagga ttggaataat attaatttat attcagagac tcgtggaata aacagatttt
      721 taggtttagt taaaacatta gaaaaaaatg atgtaccatc gattaatcga ttaaagcact
      781 gggtatctgt aacaaaagaa ttgtctaatc catctttgaa gagggaaata gaatactctc
      841 agtcagagga tttattaaaa gctttaaaat ggtcggaaga agtcaatcga cagattagtc
      901 aagctaaagg attagataaa ccctttttaa gtgctagaga tactataaaa gctctgaaaa
      961 aatatgggaa gataattatt gtaagctcag ctaataagga agctattcaa gaagaatggg
     1021 aaagacatga attattatcc ttagttgatg aactgtgttg tcaagataaa ggaaaaaaag
     1081 aagacattat tcgaagtgta attgaaaatg gttgtgattt ggataagata ttaatgattg
     1141 gtgattctcc tggtgatcta gaagctgcaa ataagaataa agttttcttt tatcctattt
     1201 tggttaataa agaaaaagaa tcatgggaaa acttaagaac tgatatttta tttaaatgtt
     1261 tatcgggaga gtatatagat attgaacaaa aatatata
//
LOCUS       DARDHA010000286         1294 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000286 DARDHA010000000
VERSION     DARDHA010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.295"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1244
                     /locus_tag="SDA10_002877"
     CDS             <1..1244
                     /locus_tag="SDA10_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEG4241710.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DARDHA010000287         1287 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000287 DARDHA010000000
VERSION     DARDHA010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.296"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..848
                     /locus_tag="SDA10_002878"
     CDS             <1..848
                     /locus_tag="SDA10_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG4241711.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          418 a          235 c          252 g          382 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttt
//
LOCUS       DARDHA010000288         1284 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000288 DARDHA010000000
VERSION     DARDHA010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.297"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            4..>1284
                     /locus_tag="SDA10_002879"
     CDS             4..>1284
                     /locus_tag="SDA10_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HEG4241712.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          431 a          249 c          246 g          358 t
ORIGIN      
        1 tatatgaatg attctatcaa aaaaatgctg agaataacag agaaagattt gatgattaca
       61 gaggtctctt acgagaccct tcagaagaaa aagacgttgg tcgtcgatgc tgttctctcg
      121 cctactcctc gtgcttgtag aagttgtggt tctactgtgg tagatggaaa cgggaaagca
      181 attatagtga aaaatggaaa aaaggaaacg attgtccgtt ttgaacaata caatcatatg
      241 cctttggtta tgcgcctaaa aaagcaacgc tatacctgta aaaactgtcg cacccattgg
      301 acggctcaaa gttattttgt ccaacccaga cattcaatcg caaatcatgt tagatataaa
      361 attgcttctt tactgactga aaaagtatct ttatctttta ttgcgaaaag ctgtcaggta
      421 tctttgacca ccgttattcg tacattgaaa gagtttaaaa gctatttacc aaagcaatct
      481 aagaagatcc tccccagagt attgatggtt gacgaatttc gttcgcatgc ttccatagaa
      541 gataagatga gctttatttg cgcggatggc gaaacaggaa aattaataga tgttttgcct
      601 acgcgtaaat tacctcgatt aacaagctat ttcttaggtt gtaccaatcc agaagaagta
      661 gaattcttgg tgacagacat gaacgccgcc tacttccagc tcaccaaacg tgttctgcca
      721 aatgcgaaag tcgtgattga tcggtttcat attgtcaaac acatgaatca agcgttcaat
      781 gagttgcgta tccgtgaaat gaatgaactt cgtaaagccg gacagaaaag ccaggcagaa
      841 aaactgaaaa agaactggcg ctttttgcta aaaaatcgtg caaacatcaa ccattatgaa
      901 tacaaaacat ggaaaagttt ccgagcacca aaatacccat ttcttactga agcaatgatg
      961 attgatcgat tgcttgagtt ttctccgccc ctaaaggagg cgtatccctt ttttcatgaa
     1021 ttagttgaag cctttcgaga caaagaccct gacttatttt tctccttatt ggcagaactt
     1081 cccgaaacgt tagatgacag ctttcgggaa aagcttcaaa accttctgac ctatgaagaa
     1141 ggcatcacca acgcaatgat ctatccttat tccaatggaa aaatagaagc gaagaatacc
     1201 cacataaaga caatgaaacg agtatcctac ggatttaaat catttgagaa catgagaatt
     1261 agaatctttt tgatcaatca atta
//
LOCUS       DARDHA010000289         1264 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000289 DARDHA010000000
VERSION     DARDHA010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.298"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..733
                     /locus_tag="SDA10_002880"
     CDS             <1..733
                     /locus_tag="SDA10_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HEG4241713.1"
                     /translation="HNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIED
                     LYYKYMETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISV
                     NFEYSHIDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVV
                     RDNAGLMAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMR
                     DELSELAKKVKVTFLTDPRWVRIDK"
     gene            868..>1264
                     /locus_tag="SDA10_002881"
     CDS             868..>1264
                     /locus_tag="SDA10_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241714.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIML"
BASE COUNT          476 a          122 c          240 g          426 t
ORIGIN      
        1 tcataatgct catcctaatt atgcaagcta tctgtcatat aagaatactt taacaattga
       61 agcaatgaat gaaatattcg aaatgatgga tgaagaaaaa aaggtatctt atgataaaca
      121 atatatagaa gatttatact acaaatatat ggaaactggg aaaatacagg aggaatatat
      181 ggaagaactg agaaaatttc tctcagacaa aaaaattcta ctgattgcac caggaaaaag
      241 ttctgtagat gaggtagaaa gaataaaaga gtttgttacc aaagaagaag tcgtaataat
      301 tagtgtaaat tttgaatatt ctcatataga tatagattac gtatttttaa gtaatttaag
      361 acgttttcat gaattaccga aagaaagcag aactaaatgt attgttacat caaatataca
      421 agcatcggat gtatatttac ggacgagtta taaaaagttg ttatctgaaa gggaagttgt
      481 aagagacaat gcaggattaa tggcaattag atttttagcc gatatgaatg tggatgaaat
      541 attcttagct ggatttgatg gatattcaca taatgaagat gagaattatg gtgagcaatc
      601 catggaaatt ataacaaaat ttgcattatt agatgcaatg aacgtgcaaa tgagagatga
      661 attgtcagaa ttagctaaaa aagtaaaggt gacattttta acggatccac gttgggttcg
      721 aattgataag tagtagtaat aatgttttta aaattttgat aaagagatag tgagagaata
      781 aaaatgggta aagtcttaaa taaaaaagaa gaaaaatatt taagggacga tggggcgaga
      841 tgaaactcta attttgaact tttaagaatc atttctatat tgtgtattgt tgcttaccat
      901 gctaatttgc gtttaaatta taatgcagac ggaattatag tacatggttc attaactgct
      961 aaccagattt tcttgaatta tattggtatt tggggtcagc taggagttaa ttgttttata
     1021 tttatttcgg catatttttt aagtgagaaa agaaacattc actttgaaaa tgttttggga
     1081 attgtattag aatgtatttt ttatagtgta ttagtttatg gaattctttg tgcatgtgga
     1141 atagaaacat tttcattgaa cagctttgta aaagaaatat tatcatttgc tctaggtaga
     1201 tattggttta ttacatccta tttagcattg tatattctaa gtccgtattt aaatatcatg
     1261 ctta
//
LOCUS       DARDHA010000290         1246 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000290 DARDHA010000000
VERSION     DARDHA010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.299"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            200..742
                     /locus_tag="SDA10_002882"
     CDS             200..742
                     /locus_tag="SDA10_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241715.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="SDA10_002883"
     CDS             898..>1246
                     /locus_tag="SDA10_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEG4241716.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DARDHA010000291         1235 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000291 DARDHA010000000
VERSION     DARDHA010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.300"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            627..1013
                     /locus_tag="SDA10_002884"
     CDS             627..1013
                     /locus_tag="SDA10_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG4241717.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            1107..>1235
                     /locus_tag="SDA10_002885"
     CDS             1107..>1235
                     /locus_tag="SDA10_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4241718.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQD"
BASE COUNT          457 a          190 c          178 g          410 t
ORIGIN      
        1 tctgtcaaaa taagctaact tacttcatta taaaatatcc acttatcgct ttcatgtata
       61 ttccaccgta aaaagtacat tttatcaccg agaactcatc atttttcatc atgatgaata
      121 ctatttacat atatctagtt caaagacaat aattattatg atccgttatt atctaagcac
      181 tatacagata ataggaattt ttcgtttcta aatattccaa tttgggaatc caataaaaat
      241 tttcgttcaa atacgtgtct attcgaactc ttttctagtt gttaattatg aagggggaag
      301 aaataaaaaa tacaatctat acacgaataa gaatattatc caaaaggagg ttttagaaaa
      361 gatttattat acgtaaaaag ctcaagacat tttcttagcc ataaggacat gtttgatatt
      421 taagaagata tatgaatttt cttttctaag tataaagata actatccatt atcaactaaa
      481 aaaacatttt tgaaaataaa catcagcagt cctatcaatg catgtgagct tatagaagtt
      541 attcgcagcg cagcataatt gcattagctt cttttcgtgt tttttgcctc ttttcccttt
      601 aaatcttaat ggaggaggtg tgcaatatgt cagataacga cgaacgcgta aatgcccagt
      661 tgattcgatt ctttgaaaag gtcattttga acaccgctaa tacttattat aaaaaacaag
      721 aagaaattca aaaacatgaa caacttgatc aactcccacc atatttgttt actaaagata
      781 ctataaaatt taaacaaccg attaatgttt taaatgtgac tctccttgtt gaagatataa
      841 aattagcaaa aatccttcct ttgttgaaag aacctgaaca aatttttctt atcgaaaaat
      901 ttatctttga taaaactgat aaagaaattg gaaaggttct ggggattaca agacagggtg
      961 cgacaaattt aaaacatcga ctttacaaaa aaattcaaaa aatgctggtt taatatctgc
     1021 ataataacta attttgtatg cttctttgct cgttctcctc cttcccaaag aaatagatta
     1081 taagtccatg ggaagaaggt agatttatga attctttaaa agaagatttc attttagcaa
     1141 aggctggaaa tgaagaagct gttgaggcaa ttctcaaaag atttagctct ttgattcata
     1201 agcaatcctg gagaactgga aaatatgatc aagat
//
LOCUS       DARDHA010000292         1225 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000292 DARDHA010000000
VERSION     DARDHA010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.301"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>1225
                     /gene="aph(2'')-I"
                     /locus_tag="SDA10_002886"
     CDS             <1..>1225
                     /gene="aph(2'')-I"
                     /locus_tag="SDA10_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HEG4241719.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
                     TIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCN
                     HLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEK
                     AKEYQDIVEEYYPIET"
BASE COUNT          512 a           98 c          210 g          405 t
ORIGIN      
        1 atcattaaaa aaacattata cagagccttg ggaagatgaa gtttttagag taattattga
       61 atataacaat gttcctattg gatatggaca aatatataaa atgtatgatg agttatatac
      121 tgattatcat tatccaaaaa ctgatgagat agtctatggt atggatcaat ttataggaga
      181 gccaaattat tggagtaaag gaattggtac aagatatatt aaattgattt ttgaattttt
      241 gaaaaaagaa agaaatgcta atgcagttat tttagaccct cataaaaata atccaagagc
      301 aataagggca taccaaaaat ctggttttag aattattgaa gatttgccag aacatgaatt
      361 acacgagggc aaaaaagaag attgttattt aatggaatat agatatgatg ataatgccac
      421 aaatgttaag gcaatgaaat atttaattga gcattacttt gataatttca aagtagatag
      481 tattgaaata atcggtagtg gttatgatag tgtggcatat ttagttaata atgaatacat
      541 ttttaaaaca aaatttagta ctaataagaa aaaaggttat gcaaaagaaa aagcaatata
      601 taatttttta aatacaaatt tagaaactaa tgtaaaaatt cctaatattg aatattcgta
      661 tattagtgat gaattatcta tactaggtta taaagaaatt aaaggaactt ttttaacacc
      721 agaaatttat tctactatgt cagaagaaga acaaaatttg ttaaaacgag atattgccag
      781 ttttttaaga caaatgcacg gtttagatta tacagatatt agtgaatgta ctattgataa
      841 taaacaaaat gtattagaag agtatatatt gttgcgtgaa actatttata atgatttaac
      901 tgatatagaa aaagattata tagaaagttt tatggaaaga ctaaatgcaa caacagtttt
      961 tgagggtaaa aagtgtttat gccataatga ttttagttgt aatcatctat tgttagatgg
     1021 caataataga ttaactggaa taattgattt tggagattct ggaattatag atgaatattg
     1081 tgattttata tacttacttg aagatagtga agaagaaata ggaacaaatt ttggagaaga
     1141 tatattaaga atgtatggaa atatagatat tgagaaagca aaagaatatc aagatatagt
     1201 tgaagaatat tatcctattg aaact
//
LOCUS       DARDHA010000293         1215 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000293 DARDHA010000000
VERSION     DARDHA010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.302"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            414..947
                     /locus_tag="SDA10_002887"
     CDS             414..947
                     /locus_tag="SDA10_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEG4241720.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DARDHA010000294         1195 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000294 DARDHA010000000
VERSION     DARDHA010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.303"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1076
                     /locus_tag="SDA10_002888"
     CDS             <1..1076
                     /locus_tag="SDA10_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEG4241721.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DARDHA010000295         1169 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000295 DARDHA010000000
VERSION     DARDHA010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.304"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>1169
                     /locus_tag="SDA10_002889"
     CDS             <1..>1169
                     /locus_tag="SDA10_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HEG4241722.1"
                     /translation="QKKKTLIVDAVFSPAPHTCRNCGSTVVDGNGKVIVVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSKRILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGQLIDVLPTRKLPRLTSYFLGCANPEEVKFLVTDMNAAYFQLTKRILPNAKVV
                     IDRFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKDPDLFFSLLAELP
                     ETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGFKSFE"
BASE COUNT          384 a          230 c          228 g          327 t
ORIGIN      
        1 ttcagaagaa aaagacgttg atcgtcgatg ctgttttctc acctgctccc catacctgta
       61 gaaattgtgg ttctactgtg gtagatggaa acgggaaagt aattgtagtg aaaaatggga
      121 agaaggaaac gattgtccgc tttgaacaat acaatcacat gcctttggtt atgcgcttaa
      181 aaaagcagcg ctatacctgt aaaaactgtc gaacccattg gacggctcaa agttattttg
      241 ttcaacccag acattcaatc gcgaatcatg tcagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa attgtcaggt atctttgacc accgttattc
      361 gtacgttgaa agagtttaaa agctacttac caaagcaatc taagaggatt ctccccagag
      421 tattgatggt tgatgaattt cgttcgcacg cttccataga agataaaatg agctttattt
      481 gcgcagatgg cgaaacagga caattaattg atgtcttgcc tacgcgtaaa ttgcctcgat
      541 taacaagcta tttcttaggc tgcgctaatc ctgaagaagt aaaattttta gtcacagaca
      601 tgaacgccgc ctacttccag cttaccaaac gtattctgcc aaatgcgaaa gtagtgattg
      661 atcggtttca tattatcaaa cacatgaatc aagcctttaa tgagttgcgt atccgtgaaa
      721 tgaatgaact tcgtaaagta ggacagaaaa gtcaggcaga aaaactgaaa aagaactggc
      781 gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca tggaaaagtt
      841 ttcgagcacc aaaataccca tttcttacgg aagcgatgat gattgatcga ttgcttggct
      901 tttctacgtc cctgaaggag gcgtatccct attttcatga attagttgaa gcctttcgag
      961 acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg ttagatgacg
     1021 gctttcggga aaagctccaa aaccttctga cctatgaaga aggcatcacc aacgcgatga
     1081 tctatcctta caccaatgga aaaatagaag cgaagaatac ccatataaag acaatgaaac
     1141 gagtatccta cggatttaaa tcatttgag
//
LOCUS       DARDHA010000296         1137 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000296 DARDHA010000000
VERSION     DARDHA010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.305"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..604
                     /locus_tag="SDA10_002890"
     CDS             <1..604
                     /locus_tag="SDA10_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG4241723.1"
                     /translation="INFDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKK
                     NLSSPFLVMQEMDYQFFTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLS
                     GGEKQRLLVSIASLSKTNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPE
                     FLYKTCNRIIYLKNGGIKKDIKLRVEDSTLIEQIFNDITK"
     gene            622..>1137
                     /locus_tag="SDA10_002891"
     CDS             622..>1137
                     /locus_tag="SDA10_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEG4241724.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASL"
BASE COUNT          385 a          142 c          228 g          382 t
ORIGIN      
        1 cataaatttt gatgtcaaag ttggagatgt tattggtttg gtagggaaaa acggtgtggg
       61 gaaaacaacc ttactaagaa tactcatgaa gattattcgc cctaccaaag gaaagataat
      121 cgaaaataaa aaaaatttgt catcgccgtt tttagtaatg caagaaatgg attaccaatt
      181 tttcacagaa tcagtgagga gcgagttagc cctggggaat gaaatagtta gtaaagatca
      241 gcaagaaaaa attttaaaaa aaatggaact ttataaaatg aaagatcaat taccgtttga
      301 tttatccggt ggagaaaaac aaagattatt ggtttctata gcttcattgt caaaaactaa
      361 tttatttcta tttgatgaac ctacaagtgg gttggattat attaatatgg aaaggattgc
      421 tgaccttatt aaagacttaa aagaaaaggg tgcagtagtt atagctactc atgatccgga
      481 atttctttat aaaacatgca atagaattat ttatttaaaa aatggtggaa taaaaaaaga
      541 tataaaactt agagtagaag attcaacttt gatagaacag atttttaatg acataacgaa
      601 atagaattga ggagaatata tatgaaaaca aaagattata tttttattgg tttagctaca
      661 gttatttccc tagtaattta ctttttttca ataatggtta gtagtattgg tggggcgttt
      721 ggtcacagca tatcccccgg gatttttgga ttattatctg gaatcatatt tgtttatatt
      781 agctataact accctagaaa aggaattttt actatttaca caattgtttt attgttattt
      841 tttacgttaa tgggcggggc gtacttacct tggtttatca gttcgattag tatggcgatc
      901 ttagctgata ttattctatc tgtatttgga tatgatcgag caattcctca agttgcgtct
      961 tgggctctaa tgcaattagg cagtgctgcc ggacagtgga ttcctatatg gttctttaca
     1021 gatcgttttc gtcaagattg gatagataaa ggacaatctg ctgcaacaat ggatgcaatg
     1081 atccactatg cggtaggtat atggggaatc atttcagtgt tagttgtagc aagttta
//
LOCUS       DARDHA010000297         1133 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000297 DARDHA010000000
VERSION     DARDHA010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.306"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="SDA10_002892"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="SDA10_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEG4241725.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          336 a          231 c          194 g          372 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cat
//
LOCUS       DARDHA010000298         1105 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000298 DARDHA010000000
VERSION     DARDHA010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.307"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1087
                     /locus_tag="SDA10_002893"
     CDS             <1..1087
                     /locus_tag="SDA10_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4241726.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          402 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaa
//
LOCUS       DARDHA010000299         1098 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000299 DARDHA010000000
VERSION     DARDHA010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.308"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(70..684)
                     /locus_tag="SDA10_002894"
     CDS             complement(70..684)
                     /locus_tag="SDA10_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG4241727.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
BASE COUNT          340 a          222 c          202 g          334 t
ORIGIN      
        1 tttttttcaa acgctccatt tttatgtgga acattatttt tttaaggaac tttgtcaaca
       61 tgtggataac tattttccac ttgtgtcctg gactagctgt tttgctgtaa aatacaaaac
      121 ttgctgcagc aaaaagatcg ttgcaagaga ggctgttccg cagatcgctg gatctagatt
      181 tggcaaagtc aagactgcat catgtaaatt ggcgtggttg tccaaaacca aatcagcaaa
      241 ttcaaataat cgcaaaccac tgctatgttg cgatttgata cttcgtgaca aatcaaatcc
      301 agtaacaatg attaatggat gaccattttc tttgatccac tgagctagtt cgatgattgc
      361 cgggtttctt ccttcattcg aaagaagaag aaaaatgtct tctgatttga cagaaagctg
      421 atcaataatt gcctgtcctg caccttctag cgtttcatag acacctccgg ctggattttc
      481 aatcaatata cttggtacaa gatccggggt ttgatcccaa aattcaaatg ctacaccact
      541 tagtgtgcgg ctagcatata tctgcaatct accaccttga ttaatttgtt tcgcgatcca
      601 agtgccagct tgaagcatca gcggctcttc tatccttaat ttctcttcca attggtgata
      661 atagtcaaaa taacataaac tcatttatct ctcccgccta gctatacttg acatgaatag
      721 aaacagctgc ctaatctaag gaagtacata aaaaaacagt agttagacag taacacatgt
      781 ggtctttgct gtttcaaaca caaaaaaggc attgaacgaa tacgcccaat accttgacat
      841 agcaaataaa acaacgtgca gaccatggaa aatatctaca taggggtaga attttcaccc
      901 tagattaggt acaataaatt aggtcgatgc actgcattga ctatggcaaa atgatcggtc
      961 agggtgtatg tgtgtgactg gtcattccct gattcttagc gccaactaag ggtcagggag
     1021 ccttttttta ttcagctgtt tctatctgaa tttattgtaa gtaatctctt atccaagtgc
     1081 aagcaagaaa atacaaaa
//
LOCUS       DARDHA010000300         1097 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000300 DARDHA010000000
VERSION     DARDHA010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.309"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..153
                     /locus_tag="SDA10_002895"
     CDS             <1..153
                     /locus_tag="SDA10_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEG4241728.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="SDA10_002896"
     CDS             159..575
                     /locus_tag="SDA10_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241729.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="SDA10_002897"
     CDS             681..944
                     /locus_tag="SDA10_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG4241730.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1097
                     /locus_tag="SDA10_002898"
     CDS             944..>1097
                     /locus_tag="SDA10_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG4241731.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLGSV
                     AKFENQT"
BASE COUNT          416 a          139 c          223 g          319 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcggtt ctgttgcgaa
     1081 gtttgaaaat caaacgc
//
LOCUS       DARDHA010000301         1089 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000301 DARDHA010000000
VERSION     DARDHA010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.310"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            66..851
                     /locus_tag="SDA10_002899"
     CDS             66..851
                     /locus_tag="SDA10_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HEG4241732.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            844..1086
                     /locus_tag="SDA10_002900"
     CDS             844..1086
                     /locus_tag="SDA10_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241733.1"
                     /translation="MTNNILISIYPRYMEKIINKQKNFEFRNFYLTSDPYNIFWIYET
                     IPTKALKYKLIVKNPITKLKKNQHYFLGDDEFEQLV"
BASE COUNT          427 a          163 c          177 g          322 t
ORIGIN      
        1 cccttttaga aaaagcaaaa aaaaaagaaa aaatcttatt cttgataaca agctacgtga
       61 cgtatatgcc taacaaaaat agaacttttg aatcaatctt aaatgaaggt ttaaccacag
      121 aaccggcatc cattattttt gatgaagtta tcgatgacgt tgaagatagt aagtatcata
      181 aaaatttaaa agaacttttt tattttgcac tcaacaatgg accaagtttg ttcactggta
      241 tatcagtacc tgatagtgat aatccattaa ttatggcaaa aatttactta acaaaatggt
      301 gtaataaata catcagggat aggaataacc cagcattaaa aaaacctctt aaaacatttg
      361 gcgaaaaaga tgctgcttta actactagag tagcagcaaa tgctaatatt gatgatcaaa
      421 aagtattgaa tgactacttg cgtggacatt ttttatatat gagcgctgag aacatgaacg
      481 gaagtatttt agaggaatat ttagccgaag tgttagaacc tgaaggttgg atttggtgcg
      541 ccggctctgt atatcgagct gttgatttct gttatttagg aacctcacca attcttttac
      601 aagtgaaaaa taaatacaat acagaaagca gttcgagttc agctataaga gtaggaacaa
      661 caattagaaa atggaatcgt ttaaataagt cgacaaaaat ttctggtttg gatagcccta
      721 tccccaattg gaaagcatta atagaaatga ctgaagctag taaggaactg gcagctaagt
      781 taactgaaaa tagttattta gcttatatta atgaaaaatc cacacgagaa ttatggacac
      841 tcgatgacta acaatatcct tattagtatc tatccacgat acatggaaaa aattataaac
      901 aaacaaaaaa actttgaatt tagaaatttc tatcttacct cagatcctta taatatcttt
      961 tggatttacg aaacaatccc caccaaagct ttaaagtata aattgattgt aaaaaatcca
     1021 attactaaac ttaagaaaaa tcaacactac tttttaggag acgatgagtt tgaacaatta
     1081 gtttaaaag
//
LOCUS       DARDHA010000302         1085 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000302 DARDHA010000000
VERSION     DARDHA010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.311"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            51..998
                     /locus_tag="SDA10_002901"
     CDS             51..998
                     /locus_tag="SDA10_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEG4241734.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          347 a          168 c          233 g          337 t
ORIGIN      
        1 ttcttcggag cttcaagata tctataatta tttatatcgg aggtaaaagg atgagctgtt
       61 atcttatcac cggtggggct ggcttcattg gttctacctt agccaatcat ttaggagaag
      121 aacatgtggt aatcgtcgtc gatgacttat caatgggaaa agtagaaaat cttgatatga
      181 cgaaaaatat cacttttatt gagggagatg tcgctgagaa atccttgatg gaagagatca
      241 tgaaagctaa taaatttgat tatatctttc atttagcagc agttgctagt gtggcagatt
      301 cagttgcccg accagtagaa acacatcgtg tgaattttga aagtgtatta atgattttag
      361 aattgatccg caagtatcaa cctgatttaa aacggatcgt gtttagttct tcagcggctg
      421 tttatgggga tgaaccgact ttaccaaaaa aagaagagtc cgtgattcgc ccattgacgc
      481 cttatgcggt tgataaattc gctgcagaac aatatgtgct agattattgt catttatatg
      541 atgtacccgg tagcgcagtt cgttttttca atgtctatgg tccaaatcaa aatccgaatt
      601 ctccatacag tggtgtgatt tctattctcg tagatcgcta taagaaacaa atcgtaggag
      661 aagcaacttc atttacatta tttggtgatg gaagccagtc tagagacttt gtttatatag
      721 atgatgtaat ccaagcatta cttttagttg cgaatgagga agctgcactg gggaaacaat
      781 tcaatgttgg aacgggtaaa gcaaccacac ttttggaatt gattcatgca atcaatgaaa
      841 ttctcggtgt agaattgaca cttgaataca aagaagagcg ttcgggtgat atccatgact
      901 ctctagcaga tattactaag atccagtcaa ttggctatca accgaaatat ggtattttaa
      961 gtggaatgac taattatttg aaaactgaaa tcaattaaaa catactaaga taagattaga
     1021 gggtaaattc aggctctttg tcaataagga ctgatgagtt ttgtaaaatc aaatctggga
     1081 tagaa
//
LOCUS       DARDHA010000303         1066 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000303 DARDHA010000000
VERSION     DARDHA010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.312"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..392
                     /gene="spx"
                     /locus_tag="SDA10_002902"
     CDS             <1..392
                     /gene="spx"
                     /locus_tag="SDA10_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEG4241735.1"
                     /translation="PSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKKILAATEG
                     GVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRILIGFNEDE
                     IRSFVPREIRCVARNLIYLQNNNILNS"
     gene            406..762
                     /locus_tag="SDA10_002903"
     CDS             406..762
                     /locus_tag="SDA10_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEG4241736.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          368 a          162 c          205 g          331 t
ORIGIN      
        1 cgcctagctg tacttcctgt agaaaagcaa gagcatggtt gcaagcgaat caacttgaat
       61 ttgaagagaa gaatattttt gccgaaccgt taacagaaag tgagatcaaa aagatcttgg
      121 cagcgactga aggaggagtt caagatatta tttccactcg gtcgaaaatt tacgaaaagc
      181 tgggcataga ttttaacgaa ttaactttaa agcaaatgat cgtcttattc aaagaatatc
      241 cttcattatt gagacgacct attttgattg atgaaaaaag aatactaatc ggatttaatg
      301 aagatgagat ccggtcattt gtccctcgtg aaattcgatg tgttgccaga aatctgatct
      361 atttacaaaa caataacatc ttaaattcat agggagggag ccttcgtgga agtgaaagtc
      421 tatggatcag cgcactgtgt aacaagtctg aaggcgaaac aatggttaga acaccatcaa
      481 ttgccttatc aatttgttga cttagaagaa cgcgatctaa gtacagaaga agcagaagaa
      541 ttatgcagtt ttgaagaagt gcaagcagaa cagctatttg cgacttggtc tgaggctttt
      601 cacaacattg cgattgacct ttcctgtgta ggaaaaaaac aactgatttt tttatgcaca
      661 caacatacca atctcctcag acgtcctttg attattatcg atgaagttct atttattgga
      721 tacaatgagc agttgatgga acagcttatc ttaaataaat agactttaac tgttttaatc
      781 aagtcactga gcatttcata cagttgtaac agtcgaacac ttagcatcaa gcgtgttatg
      841 tgttcttttt ttgactttat ttttctacta tagagacatg ctcaatttat gtggtcaaaa
      901 ttcaaatcta ggtggggata tagagttaaa aatatattga catgtgtaaa tttacatgat
      961 atattcattt ctgttgtttt aagtagcaaa gcaataaaat agtattttaa aaaacatata
     1021 aaatagttgt tgacatgtta gaaatgacat gataagataa ctgagt
//
LOCUS       DARDHA010000304         1000 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.314, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000304 DARDHA010000000
VERSION     DARDHA010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.314"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            78..193
                     /gene="rrf"
                     /locus_tag="SDA10_002904"
     rRNA            78..193
                     /gene="rrf"
                     /locus_tag="SDA10_002904"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            203..276
                     /locus_tag="SDA10_002905"
     tRNA            203..276
                     /locus_tag="SDA10_002905"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:237..239,aa:Asn,seq:gtt)
     gene            316..405
                     /locus_tag="SDA10_002906"
     tRNA            316..405
                     /locus_tag="SDA10_002906"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:350..352,aa:Ser,seq:gga)
     gene            420..491
                     /locus_tag="SDA10_002907"
     tRNA            420..491
                     /locus_tag="SDA10_002907"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:453..455,aa:Glu,seq:ttc)
     gene            507..579
                     /locus_tag="SDA10_002908"
     tRNA            507..579
                     /locus_tag="SDA10_002908"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:540..542,aa:Val,seq:tac)
     gene            589..661
                     /locus_tag="SDA10_002909"
     tRNA            589..661
                     /locus_tag="SDA10_002909"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:622..624,aa:Asp,seq:gtc)
     gene            697..772
                     /locus_tag="SDA10_002910"
     tRNA            697..772
                     /locus_tag="SDA10_002910"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:Phe,seq:gaa)
     gene            774..854
                     /locus_tag="SDA10_002911"
     tRNA            774..854
                     /locus_tag="SDA10_002911"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:Tyr,seq:gta)
     gene            861..934
                     /locus_tag="SDA10_002912"
     tRNA            861..934
                     /locus_tag="SDA10_002912"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:893..895,aa:Trp,seq:cca)
BASE COUNT          239 a          223 c          271 g          267 t
ORIGIN      
        1 actcggaaaa aaacttatgt gacttcaaat ccagttttga gtgaacaaaa tttactcaat
       61 aaaagataac accacagtgt ggtggcgata gcgagaagga tacacctgta accatgccga
      121 acacagaagt taagcttctt agcgccgatt gtagtgaggg gttgcccctt gtgagagtag
      181 gacgtcgcca cgctgatgtt attccggcat agctcagttg gtagtagcgc atgactgtta
      241 atcatgatgt cgtaggttcg agtcctactg ccggagttct cattttgaga aagagtattg
      301 agcttctttc actctggaga gttgtccgag aggccgaagg agcatgattg gaaatcatgt
      361 agatggtgcc aaactgtctc aagggttcga atcccttact ctccgtagca aatttacctg
      421 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
      481 cgtacgggtc attttaattg aatgttggag gtttagctca gctgggagag catctgcctt
      541 acaagcagag ggtcagcggt tcgatcccgt taacctccat aatgaactgg ttccgtggtg
      601 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
      661 gtcgttcttg ttattcaatt aataatttta aaatatggcg cggtagctca gttggtagag
      721 caacggattg aagctccgtg tgtcggcagt tcgattctgt cccgcgccac catggaggag
      781 tagcgaagtg gctaaacgcg acggactgta aatccgttcc ttagggttca gtggttcgaa
      841 tccactctcc tccattttat aggggcatag tttaaaggta gaacagcggt ctccaaaacc
      901 gttagtgtgg gttcaattcc tgctgcccct gccatggcga atgtggcgaa gtggttaacg
      961 caccggattg tggctccggc attcgtgggt tcgattccca
//
LOCUS       DARDHA010000305          995 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.315, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000305 DARDHA010000000
VERSION     DARDHA010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.315"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(26..913)
                     /locus_tag="SDA10_002913"
     CDS             complement(26..913)
                     /locus_tag="SDA10_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEG4241737.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          321 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaa
//
LOCUS       DARDHA010000306          994 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.316, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000306 DARDHA010000000
VERSION     DARDHA010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.316"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>994)
                     /locus_tag="SDA10_002914"
     rRNA            complement(<1..>994)
                     /locus_tag="SDA10_002914"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DARDHA010000307          987 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.317, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000307 DARDHA010000000
VERSION     DARDHA010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.317"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            354..987
                     /locus_tag="SDA10_002915"
                     /pseudo
     CDS             354..987
                     /locus_tag="SDA10_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          337 a          181 c          180 g          289 t
ORIGIN      
        1 agaaaatgta gttatatatt taaattttta ccagaaagaa atgaacacct tcaattatct
       61 aaatctcgaa aatggttaga tgtaatcatc aggaaaaaca atcttaaacg aattactatt
      121 catggtctta gacacacaca tgctagcttg cttttagcaa gctagtgcaa atattaaaga
      181 tgtgcaagag cgtttaggcc actcttctat tcaaatcatt atggatctat atattcatat
      241 tacagacaaa agaaaagaag aaaccgtagc gcaattcgca aaatataggc tctataattt
      301 atgggactga tgaatcaaaa ttgattcttc agtcccattg ttattttagg tatatgattc
      361 gtacattgaa agagttgaaa aactatcttc cgaagccgtc aaaaaggacc ctgccaaaag
      421 tattgatggt ggatgaattt cgttcgcacg cttcaattgt ggataaaatg agctttattt
      481 gtgcggatgg cgagactgga aaactcattg atattttgcc tacacgaaaa ttgtctcgat
      541 taacacacta ttttagtcgc tgtgcaaatc ctgaagcggt ggaatattta gtgacagata
      601 tgaacgccgc ctacttccaa ctgacaaaac gtgtcttacc aaatgcgaaa gtcgtcattg
      661 attgttttca tatcatcaaa catttgaatc aggcgttcaa tgattttcgt gtacaagaaa
      721 tgaaaagatt acaccagtta gggcaacgac gggaagccga gaaagtgaaa aagaactggc
      781 gctttcttct aaaaaaccgt caaaaaatca atcactatga ttacaaaaca tggcgcagtt
      841 ttcgtgcacc taaatacccg ctactcactg aagcaatgat gattgatcga ttgctggcat
      901 tttcaacgcc tttaaaggac gcctatactt ttttccatga gctgaccgaa gcctttagag
      961 acaaggaatc agacttattt tttacct
//
LOCUS       DARDHA010000308          972 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.318, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000308 DARDHA010000000
VERSION     DARDHA010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.318"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>972
                     /locus_tag="SDA10_002916"
     CDS             <1..>972
                     /locus_tag="SDA10_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG4241738.1"
                     /translation="DSNYEVILIDKKSEISYLECGTSLFIKGEVSNLEDLFYQNRMTL
                     NSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQEYDKVILATGSKQIMLDIPGNNL
                     ANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYIGVEIAEAIRKRGKEVYLFDVADR
                     VLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYLGRKKIEKIVTTSGTQYSMDMVVQ
                     AIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKDIYAIGDCATTFFTSINRSSVDFS
                     ISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFNYNFYAAGLNLKTA"
BASE COUNT          378 a          116 c          169 g          309 t
ORIGIN      
        1 cgattcaaat tatgaagtta ttctaatcga taaaaaatca gaaattagtt acttggaatg
       61 tgggacatcg ttatttataa aaggagaagt aagtaattta gaagatcttt tttatcaaaa
      121 tagaatgaca ctaaattctg agataaaaaa gttatttcta gagacagaag ttgacaaaat
      181 tgatattcaa aataaaaaga ttagttgtgt aaaaaaagat ggtacaagat tctctcaaga
      241 atatgacaaa gttatattag ctacaggttc aaagcaaatt atgctagata taccggggaa
      301 taatttagct aatatttttt cgataaagaa ttttccaaat gcaaaagaga tttttaaagc
      361 tttggatgat agtactatta aaaaagtagg aattattgga gcaggatata ttggagttga
      421 aattgctgaa gccataagaa aaagagggaa agaagtatat cttttcgatg ttgcagatag
      481 agtgttgtcg acttattacg accgtagttt ttcagataaa gttgaagaaa tactatcaaa
      541 aaatggaata catctttgtc tatcagaaag tcctaaaaaa tatttaggaa gaaaaaagat
      601 tgaaaaaata gtcactacaa gtggtaccca atactctatg gatatggtcg tacaagctat
      661 aggatttcta tctaatagtc caattgggga aaaggaactc ttgagagatg ttagtggagc
      721 atatctaact aatgaatacc agcaaacaaa tataaaagat atatatgcta ttggggattg
      781 tgctacaact ttttttacgt ccattaatag aagtagtgtt gatttttcta tttctaatgc
      841 tctaagaaca gcttatattg caactcaaca tattaacaat caagatttta caccttctgg
      901 ttcccaattt acaaatggct tttctatatt taattacaat ttttacgcag caggtttgaa
      961 tttaaaaaca gc
//
LOCUS       DARDHA010000309          965 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.319, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000309 DARDHA010000000
VERSION     DARDHA010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.319"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>965)
                     /locus_tag="SDA10_002917"
     CDS             complement(<1..>965)
                     /locus_tag="SDA10_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEG4241739.1"
                     /translation="DVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTI
                     VTIIANIFFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREM
                     LNYSIPLIATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAW
                     QISAIKEYGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYV
                     PFLLIANVLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATI
                     ATVICSYVIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIY"
BASE COUNT          356 a          185 c          117 g          307 t
ORIGIN      
        1 atatatttct atgattgcct gaacaaacaa taatccccaa gattccacaa caatatgata
       61 atgctttata aaaatatctt ttcccacagc tcgtttacga acaacatata ttacatatga
      121 acatataaca gtggctattg tggcaccttg aacccctatc ttataaacaa ggactacact
      181 cataatcaca ttaactaatg cccctatgat tgcagaccac atcatggatt tagaatcttt
      241 ttttgctgat aagataggtc ctaacagacc tgatgcacaa tttaaaacat tagctattaa
      301 taaaaatgga acatatttcc acgcttcata aaagtctttc gcataaagaa tatgtgaaaa
      361 cggtctagtt aatatgataa gacttgaaca ggttatgcac attaacaaat taatcataac
      421 aaatgtattt ccataaaatt ttgcagtctc attatctcca tattctttaa ttgcagaaat
      481 ttgccaggcc tgtataaata ttccctgcaa agtattaatt atttgaggta ttttatatgc
      541 cacagacaat aagccattcg ccgagacacc tactataaag gaaaccacat acctatcagc
      601 tgtactatta atccaccatc caacaacagt tgcaattaat ggaatcgagt aatttagcat
      661 ttctctcttt agctgaggat ctttagcctc aaaatcgata tattcccata ttctcactaa
      721 tactgacaga taagtagctg aaataatctg tgataaaata tttgcaatga aaaaaccaat
      781 taatccccat ttcagaataa gcaagaagaa aatattggca attattgtta caattgtgct
      841 gataatacct gctatgccca tagctttaac tttttctaac cctttagcaa attgtattag
      901 aaattgattc catacatagg atatataata caaaaaaata tatttttcta atccataaac
      961 atctt
//
LOCUS       DARDHA010000310          962 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.322, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000310 DARDHA010000000
VERSION     DARDHA010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.322"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..861
                     /locus_tag="SDA10_002918"
     CDS             <1..861
                     /locus_tag="SDA10_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEG4241740.1"
                     /translation="VNKLLKKESFSIVHNQVYFGGGINLWLAKKNGIRQRIAHSHATE
                     DGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSK
                     NSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEG
                     SLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAK
                     LKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTE
                     QMESIYSRDH"
BASE COUNT          370 a          109 c          185 g          298 t
ORIGIN      
        1 gtgaataagt tactaaaaaa agaatctttt tctatagttc acaatcaggt ttattttggc
       61 gggggaatta atttatggtt agccaaaaag aatggaatca gacagagaat tgctcatagc
      121 catgcaactg aagatggaaa atctcaaaat attgtaatga atgtattgcg taaattttta
      181 accaaattgt tgttacaaaa tgcaacagac taccttgccg ttagtcaaga agctggtgaa
      241 agtctgtttc aaaaccatcc ctttgagata gttcataatg gtattgattt agagctttat
      301 agtaaaaatt cagaagctaa ggttaataag agaaaagaat tggatatctc aatgagtact
      361 tttgttgtgg gaaacattgg acgattagag aaacaaaaaa atcaatctta tttgttagag
      421 atttttttag aaatactaaa aatgaattct gattcttact tgattttagt aggtgaaggg
      481 tcacttagaa ataaactgaa agccaaagca gtagaattgg atatagagaa aagagtaaaa
      541 tttttaggag aaagaaacga tattcccgag ttattatctg tgatggatgt ttttgtaatg
      601 ccttctcttt atgaaggtct gccaatggtt ggattagaag cacaagcagc aaaattaaaa
      661 ttagtcttag cagatactat ttccaaagat acaaagttaa caaataatgt ggattttgta
      721 tcattagatg aaacaccaca agaatgggca aaaaaagtaa taaaagagcc gtatgctaat
      781 aaaataacac aagaactaaa gagttatgat gtttcgtaca ctactgaaca aatggagagt
      841 atatatagta gagatcatta aaaatgttca agatatttta ggagtgatta tttttgaaaa
      901 tatgttattt gatattggca tacaatgata ttggcaactt aaagagattg gtcaatcgat
      961 tg
//
LOCUS       DARDHA010000311          959 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.323, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000311 DARDHA010000000
VERSION     DARDHA010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.323"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(107..511)
                     /locus_tag="SDA10_002919"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="SDA10_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="SDA10_002920"
     CDS             complement(570..>959)
                     /locus_tag="SDA10_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4241741.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DARDHA010000312          946 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.324, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000312 DARDHA010000000
VERSION     DARDHA010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.324"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(36..>946)
                     /locus_tag="SDA10_002921"
     CDS             complement(36..>946)
                     /locus_tag="SDA10_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241742.1"
                     /translation="KDLKQYYPLSAYEKKKLFHFLAINLLAEQADSLDIASNQSLFFS
                     DIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNEDETIEWLNERFIESFEN
                     QFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFFRENYPAFYCYLIEYIN
                     DLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLS
                     LYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDPPRAVDWGNLTKILLTI
                     QEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          306 a          161 c          145 g          334 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat ctttta
//
LOCUS       DARDHA010000313          936 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.325, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000313 DARDHA010000000
VERSION     DARDHA010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.325"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(21..296)
                     /locus_tag="SDA10_002922"
     CDS             complement(21..296)
                     /locus_tag="SDA10_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4241743.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            528..>936
                     /locus_tag="SDA10_002923"
     CDS             528..>936
                     /locus_tag="SDA10_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4241744.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEP"
BASE COUNT          327 a          161 c          142 g          306 t
ORIGIN      
        1 ttttagtctt atttgtttac tcaaccttta taccgttcca tgaaactatc aatatcagat
       61 tttttaaatc taatgaccgt atcaattttt ataacaggta acccgtattg agaaacatat
      121 tttctaatcg ttcctactga tacacctaga tattcacttg cctgctgata gtccatatac
      181 tctagagtga ctccagaaat aatgtcttct agcataggct ttagcactgt aattatctct
      241 ttcacttgtt gttcaacttg tatagaaaca atgctatcaa tgatttgtag agacatattt
      301 ccccctcctt tcgtgattgc ttgaacaaat tttctaagaa ttatcttcgt attttttgct
      361 caaaataaaa gtattttgtt attaaccgat taatcaaaca aaaaatgatt accagttcat
      421 cgacatacac ataatacctc gataaaaaag gggtgtcaac agctttgtaa aatttatttg
      481 caacacccct tttttgaggt atactaattt taggaggtgt ggtcaatatg ataaaacgtc
      541 caccaataaa ttatttagaa cgaaaaaaga tactcggaac aaaaataaaa gctataagaa
      601 agagtaaaaa attaacacaa ccagcatttg gtcttatgat taacaacggt caattaattg
      661 acaaaaaaac tatctacgaa tgggagaagg gaacctattt accaatacca gagcgattat
      721 cccgaattgc tgatttggga aatatgagta tagaggagtt agtttgtggc aatgtagaag
      781 aatatatttt gggaattatt ctttatagag atagcattgt actagatggt ataacatttc
      841 cagataaaaa cctgttccag catcttcgtc aacagttccc accagtacac tctaatttag
      901 atacatggct ggatagatat tccaaattag aaccag
//
LOCUS       DARDHA010000314          923 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.326, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000314 DARDHA010000000
VERSION     DARDHA010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.326"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            86..355
                     /locus_tag="SDA10_002924"
     CDS             86..355
                     /locus_tag="SDA10_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG4241745.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            348..605
                     /locus_tag="SDA10_002925"
     CDS             348..605
                     /locus_tag="SDA10_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG4241746.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          353 a          100 c          174 g          296 t
ORIGIN      
        1 ataagaagtt agaatattaa tcatttgctt aattgtacaa tataatgtac aattgtttta
       61 tagaaataaa taaggggtga aaggaatgga agcagtagct tattcaaatt tccgccaaaa
      121 tttacgtagt tatatgaaac aagttaatga ggatgctgaa acacttattg taacaagtaa
      181 agatgtagaa gatacagttg ttgtattatc aaaaagagat tatgattcta tgcaagaaac
      241 gttgagaaca ctttctaata attacgtcat ggaaaaaatt cgtcgaggag atgaacaatt
      301 ctccaaaggt gcatttaaaa cacatgactt aatcgaggtt gaatctgatg attaaggctt
      361 ggtctgatga tgcttgggat gattatcttt attggcatga gcaaggaaac aaaagcaata
      421 taaaaaagat taacaagtta ataaaagata tcgatcgttc cccctttgct ggattaggaa
      481 aacctgagcc attaaagcat gatttatctg gaaaatggtc cagaagaatt acagatgaac
      541 atagactgat atatagagtt gaaaatgaaa cgatatttat ttattctgca aaagatcact
      601 attaaccaat cggaagtaag gaaagggtca gaaacttaaa agtttttgat ccttatttta
      661 tttaccctag tcatttaaaa agctaatata gcttagtgtt gattgttatt aatgaatgtg
      721 tttgttacgc gtattacgga tataaggtta gtaaaatcat ttctaaagtt gaggaaaagt
      781 aaatataaat ggcttaaatt tcaacaattt gaagttgaat agatatgtta taatactatt
      841 gtagtgtggg atgttagtta ctaaaggatg acgcttatat atatgactga atagaataag
      901 caataggttt aataatctat ttt
//
LOCUS       DARDHA010000315          903 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.327, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000315 DARDHA010000000
VERSION     DARDHA010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.327"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            263..886
                     /locus_tag="SDA10_002926"
     CDS             263..886
                     /locus_tag="SDA10_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG4241747.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          333 a          129 c          167 g          274 t
ORIGIN      
        1 gttttaaaat aaactaatgt ccttattttt gtgttcacat ttctgttacc taagaaatac
       61 ttaaaggagg tgatgtagcc agcaacgact acaacttgaa cccgctatct acagtttatt
      121 tgttgtttct tttgggtata ctctaatggt acaaatccat atttgcttaa agcgttgatt
      181 tttcagcggt ttttctttat tgcaaagtgt accctaagac ctgtatacta atagtaaata
      241 gattggatag gaatgatggc aaatgtattt agcttattta agagtttctt ccgacgatca
      301 aagtttagct cggcaacata agctaattga aaattggtca gtcaaaaata atattgctga
      361 agacgattta aaaattttcg aggaaaaagt ctctggaaaa aatattgaaa acagaccaga
      421 gctgcaagag cttatttctt tcatacgtga acgagatacg attattattc ctagtcttga
      481 tcgattagca agaaatagta aagatacaaa agacttactt caattgtttc gtagtaaagg
      541 tgcttctatt gaaatattag acctaccgtc gtttaatggg gtaactgacc ctgcattaag
      601 agacttgcta acaaatttag tcatagaagt gtttagctat gttgctgaaa acgaacggaa
      661 aaaaatcaaa gagcgtcaaa aacaaggcat tgaaattgca aagaaaaatg gtcgttatac
      721 tggtaaaaag attcaatacc atgccaatgc aacaggaaaa aacaaattga tttacgatga
      781 aattgttgat ggtctgaaga aaaaagaaac tatttctaaa atagcaaaga acgcaggagt
      841 tacaagaaaa accgtttatc gagtaaaaag agatttagga atgtaatatg ttccagttag
      901 aaa
//
LOCUS       DARDHA010000316          898 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.328, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000316 DARDHA010000000
VERSION     DARDHA010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.328"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            21..>898
                     /gene="vanY"
                     /locus_tag="SDA10_002927"
     CDS             21..>898
                     /gene="vanY"
                     /locus_tag="SDA10_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000925406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HEG4241748.1"
                     /translation="MLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHLENSGTSE
                     NTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLLDSNIYMS
                     KEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGYSEHNSGL
                     SLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIRYVGLPHS
                     AIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNLRYEISGN
                     NIDGVIVTVFPGSTHT"
BASE COUNT          324 a          124 c          182 g          268 t
ORIGIN      
        1 tgaagaagtt gtttttttta ttgttattgt tattcttaat atacttaggt tatgactacg
       61 ttaatgaagc actgttttct caggaaaaag tcgaatttca aaattatgat caaaatccca
      121 aagaacattt agaaaatagt gggacttctg aaaataccca agagaaaaca attacagaag
      181 aacaggttta tcaaggaaat ctgctattaa tcaatagtaa atatcctgtt cgccaagaaa
      241 gtgtgaagtc agatatcgtg aatttatcta aacatgacga attaataaat ggatacgggt
      301 tgcttgatag taatatttat atgtcaaaag aaatagcaca aaaattttca gagatggtca
      361 atgatgctgt aaagggtggc gttagtcatt ttattattaa tagtggctat cgagactttg
      421 atgagcaaag tgtgctttac caagaaatgg gggctgagta tgccttacca gcaggttata
      481 gtgagcataa ttcaggttta tcactagatg taggatcaag cttgacgaaa atggaacgag
      541 cccctgaagg aaagtggata gaagaaaatg cttggaaata cgggttcatt ttacgttatc
      601 cagaggacaa aacagagtta acaggaattc aatatgaacc atggcatatt cgctatgttg
      661 gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt tctcgaggaa tatatggatt
      721 acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg ggaaaaatat gagatctttt
      781 attatcctgt tactaaaaat accaccattc atgtgccgac taatcttcgt tatgagatat
      841 caggaaacaa tatagacggt gtaattgtga cagtgtttcc cggatcaaca catactaa
//
LOCUS       DARDHA010000317          883 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.329, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000317 DARDHA010000000
VERSION     DARDHA010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.329"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            6..>883
                     /gene="bsh"
                     /locus_tag="SDA10_002928"
     CDS             6..>883
                     /gene="bsh"
                     /locus_tag="SDA10_002928"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEG4241749.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDS"
BASE COUNT          300 a          120 c          162 g          301 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tca
//
LOCUS       DARDHA010000318          869 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.330, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000318 DARDHA010000000
VERSION     DARDHA010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.330"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>869
                     /locus_tag="SDA10_002929"
     CDS             <1..>869
                     /locus_tag="SDA10_002929"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241750.1"
                     /translation="ICVLTVFLPQITEYLNYRIFTTKVDDSVTSLSRLEFIKICASVF
                     GPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKELLTQIDREFYFLLNMFI
                     EIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEIGSTIEQSGPLKGTVEY
                     IKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQIYLGRYFKMFHRIVKT
                     LNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYIKRGFGLGIQLIGSGFF
                     GDDFDFETNQHFET"
BASE COUNT          332 a          102 c          160 g          275 t
ORIGIN      
        1 atttgtgttt tgacagtatt tttaccacaa attactgagt atttaaatta tcggatattt
       61 accacaaaag tagatgatag cgttactagt ttaagtaggc tagaatttat aaaaatatgt
      121 gcatctgtgt ttggtcctat tttgactgta atggtatttg tgaatacttt aaatatgcag
      181 aaaaaaacag agcaaaagca aaatgagctt gaagtaaagc aagctaaggc tgagaacgag
      241 agagcaaaaa aagagctgtt aacacagata gatcgtgagt tctatttttt attaaatatg
      301 tttattgaaa tacaaaaaga tcaaaatgcc aaacaagctg ttattgcttt acagaagttg
      361 gcaattttta acgaaaaggg ttttaatcaa gactatgcat ttacttctaa agatttatca
      421 gatagtgaaa ttgggagtac aattgaacaa tcaggaccgc tgaagggcac agttgaatat
      481 ataaaatttg aaacatttga tgtaagcaag tcgatgggga attatcaaac aaaaacaaaa
      541 gacgttgcta caagtttaca cgatcagaaa gtagctatat tgaatgaaca atatgaaaag
      601 atgcaaatat atcttggacg gtacttcaaa atgtttcacc gaatagttaa aacattaaat
      661 gagtattatg acgattataa tgattttgat gtgaagaggt atacaaaata tatagggaca
      721 ttaagaacac aaatttctcc agctgaattc caagtgattt tattcaattc tctatatata
      781 aaacgaggtt ttggcttagg aattcaactt attggaagtg gtttttttgg agatgatttt
      841 gattttgaaa ctaatcaaca ttttgaaac
//
LOCUS       DARDHA010000319          854 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.331, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000319 DARDHA010000000
VERSION     DARDHA010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.331"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..357)
                     /locus_tag="SDA10_002930"
     CDS             complement(<1..357)
                     /locus_tag="SDA10_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG4241751.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEY"
     gene            complement(429..587)
                     /locus_tag="SDA10_002931"
     CDS             complement(429..587)
                     /locus_tag="SDA10_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4241752.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
BASE COUNT          251 a          146 c          125 g          332 t
ORIGIN      
        1 gtattcacca aacataggta aaatatgcaa ccgaaatata tcttttgtat tactaatggt
       61 tatatcacta ggaggcctgt ttgatctaat tgtttgacct aattcatatc taggaagcca
      121 tttagattcg taaaattttt taaacgttat ttttcctttt aattctaatg agtttgcatt
      181 ctcctctctc aaaacttttt ctattttctt cttcatatca ttttcggctt taatcgccaa
      241 ttgcctattt ggaaatgttt tttgatactt ttctgatgca attcctgtta tttctttaaa
      301 cctttttgga tatctcagat tagttttata tttcccattt gaaagctttg taggcaaaat
      361 cctcactcct taaaaaaagt aaggcagtat atttcactaa ttttagtata tgcggcgaga
      421 agaaaatttc aagttatttt aaatgtattc ataaagtctt taatatcttc tacgtcaaag
      481 cgaactacac caccaactct tattactggg aaattatata tacgaatcca cttatctaga
      541 gtattattat cgattcctag aaatgcacag gtttgttttt tattcaggta gactctggat
      601 tctataaaat catctcctaa aatagtagtt atgaaatttt tgaattgctt tgggtaagca
      661 cataggaatt tcacctctcc ccagtttacg gcgagcctta cggaagtatc attatttgct
      721 gctattgtca tggcattttg ttttcccaga acaaaaagaa atctctaagt atttgtcgct
      781 aatcaggtca tggcgtactt gagtttctag ctcgaatagc agggatcgtt attcaaaaat
      841 tgtatttaat tgtc
//
LOCUS       DARDHA010000320          844 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.332, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000320 DARDHA010000000
VERSION     DARDHA010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.332"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            68..>844
                     /locus_tag="SDA10_002932"
     CDS             68..>844
                     /locus_tag="SDA10_002932"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG4241753.1"
                     /translation="MYANMVPTLWTTLISIIWKWQDYSVSLNRYYEYYSNNNKVAGTK
                     KINKINSITGQSLNFSYNKRKVLSNISFSTEMNKLFMISGENGTGKTTLMKLFTKQLP
                     VSNNQLFVNGIDINMIDNESLRRLISIATQIPYIINSSVKENILMGTKNYDKKKFSKL
                     IELLGISKFMTKLGITLDDNIYDEKILLSGGEKQKISLARALIKSASVIILDEPFRNL
                     DNETRDNLRDYILNTKKEVCYIIITHDHILDNFLDSNDEIL"
BASE COUNT          354 a           89 c          125 g          276 t
ORIGIN      
        1 atttcttatt gatgttattt tattagctaa aggaacaata agtattacta attttgtaat
       61 agtatttatg tacgctaaca tggtccctac tttatggact actttaatta gtattatttg
      121 gaaatggcaa gactattcag tgtcattaaa tagatattac gaatattatt caaataacaa
      181 caaagtggct ggcacaaaaa aaataaataa aataaattct attactggtc aatcattaaa
      241 tttttcatat aacaaaagaa aggtattaag caatataagt ttttcaactg aaatgaataa
      301 actattcatg atatcaggag aaaatggaac agggaaaaca acactgatga aattatttac
      361 taaacagttg ccggttagta ataatcaatt attcgtcaac gggatagata ttaatatgat
      421 tgataacgaa tcattaagaa ggttaatttc aatcgcaaca caaataccat atataattaa
      481 tagctccgtg aaggagaata ttttaatggg gactaaaaac tatgataaaa aaaaattcag
      541 taaattaata gaactattgg gaataagtaa atttatgacg aaattaggta ttactttgga
      601 cgacaatatt tatgatgaaa aaatattatt atctggagga gaaaaacaaa aaataagttt
      661 agctagggcg cttattaaat cagcttcagt tattatatta gatgaaccat ttagaaattt
      721 agataatgaa actcgcgata atttgaggga ttatatacta aatacaaaaa aagaagtttg
      781 ttatataatt ataactcatg atcacattct agataatttt ttggattcaa atgatgaaat
      841 atta
//
LOCUS       DARDHA010000321          843 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.333, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000321 DARDHA010000000
VERSION     DARDHA010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.333"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            106..>843
                     /locus_tag="SDA10_002933"
     CDS             106..>843
                     /locus_tag="SDA10_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HEG4241754.1"
                     /translation="MHGKITASAIARETNKLLNTYVYEAFDMPIEGETSDLINLINNF
                     CKSIDTTNGLILLVDMGSLEQMYEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPI
                     SHFYQMDFDSFQVKVQYYKGLSQKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIF
                     DFNALKKIAKEKDTMIFKNTICVLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDS
                     DSCEFCLNELVKLFTIEGASTKLRFLDS"
BASE COUNT          303 a          103 c          157 g          280 t
ORIGIN      
        1 agtttagaag tcgaagaaag taaacaagaa cttttgttga aatggatact ttttcttgta
       61 aagaagttct atgtgaaaat accagaaacc cattgtctga tagttatgca cggtaaaata
      121 acagcttctg cgatcgcaag ggaaacaaat aagttgctga atacatatgt ttatgaggct
      181 ttcgatatgc caattgaagg tgagactagt gacttaatta acctaatcaa caatttttgt
      241 aaaagtatag atacgacgaa tggactcatt ctgctagttg atatgggatc attggaacaa
      301 atgtatgaaa aaattgaatc caatgtaata ggtgatttgg taattttaaa caatgtttct
      361 acagctttag cgattgaatg tgggattcaa gtttgccaaa aaaagccaat tagtcatttt
      421 taccagatgg attttgattc gtttcaagtt aaagttcagt attataaagg attatcacaa
      481 aagaaaaatg taatagtatc ttgtctctct ggtgagggga tttctgaaaa agttaaggat
      541 attttaaaac ggtatctcga taatcaagtg gagattttga tttttgattt taacgcatta
      601 aagaaaatag caaaggaaaa agatactatg atttttaaga atacaatttg tgttctttca
      661 accactgaat tttacattca aggaatcgat tgtttaaatt tagaaaatct aattaatggg
      721 aatcaaacct tagaaaaatt aaataagtat atggactcgg atagctgtga gttctgctta
      781 aatgaattag ttaaactctt tactattgaa ggcgctagta ctaagcttcg ctttttagat
      841 tcc
//
LOCUS       DARDHA010000322          842 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.334, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000322 DARDHA010000000
VERSION     DARDHA010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.334"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(192..590)
                     /locus_tag="SDA10_002934"
     CDS             complement(192..590)
                     /locus_tag="SDA10_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEG4241755.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(587..823)
                     /locus_tag="SDA10_002935"
     CDS             complement(587..823)
                     /locus_tag="SDA10_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG4241756.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
BASE COUNT          261 a          152 c          120 g          309 t
ORIGIN      
        1 agcccactag ttctaaacca ctatttccta ctattcactc ttttgtattc cttttaaatc
       61 aattatttca aaattaatcc atattttcca tatttttatg aaaaaactat tttgtatgat
      121 taaacataaa aaaatcagca tattccttaa tgacatgctg atctaattga gaaataaagt
      181 ttttaattcg ttcattttat tgcggtttgt cgtaaataac tggttatctt ggactttagt
      241 gaatcgaaat ccaatgaact agtggtaatc attatgataa aatcaacagc ttcttgacga
      301 gaaaaagcaa ctggacaatt attcaattga agaaacaaca acatggcaac aagagcagtt
      361 cgtttattgc cattatgaaa aggatgtttt ttagctaaat taattgctaa aatcgccgct
      421 ttttcataga tttcaggata gagttcttga ttgaatactg cttgtgaagg ctgtttgacg
      481 gccatatcta atgcatttgc atcttttaca ccgatcattt cgcctggcga gtgacgtaaa
      541 ataactttcg cgttgatttt gataatttct ttagtcgaaa gatacctcat aaatcaacca
      601 aatcttttaa cgcgtcttca tattgattca tgatcgaatc aactaatgaa tcaatatcag
      661 gttcttcttg tgctttgatc attcgtacag aaccatcaga ttgaaaaaca taactgattg
      721 cgtcacctgt ctgcacaccc atgaattctt ttacttctgg tgggatagtt gttacatcgc
      781 tattgccaga ttttctaatt ttatactttt tcattttttt catatttcgc caccctctat
      841 ac
//
LOCUS       DARDHA010000323          836 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.335, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000323 DARDHA010000000
VERSION     DARDHA010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.335"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..181)
                     /locus_tag="SDA10_002936"
     CDS             complement(<1..181)
                     /locus_tag="SDA10_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG4241757.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKE"
     gene            complement(178..831)
                     /locus_tag="SDA10_002937"
     CDS             complement(178..831)
                     /locus_tag="SDA10_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG4241758.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
BASE COUNT          270 a          142 c           70 g          354 t
ORIGIN      
        1 tttcttttcc attcatatta ctagaaattt catttgccat attatgaatc ttttcttttc
       61 tcgtctccaa atatgtttta ggaaagataa aaaaaattaa tgaatgaact aatataatta
      121 ttattccaag aacagaaaat atttgtatga acatttttgg aaatatcttt aattttttca
      181 ttttctctcc aatttatatc ctacatttct tatagtagtt atacagtcta attgtaattt
      241 ttttctaagt tcttttatat atacatctat tactcgatca taaggagttt cttcgctatc
      301 tttccataca taatcaataa tttgcattct tgttaacact tgtccattat tatccaataa
      361 acattttaat acttctagtt cttttgcatt tacatctatt tcttcaccat ttattttagc
      421 tgtatagcta ttaaaattaa ccgatagatc cttatattca aacttctcta aatgtccata
      481 attcttctta attaaagaat ctattctagc ttttaagacc ggcaatgaaa atggcttttc
      541 tacatatccg tctactaaat tagtaaatgc atcaatttta tattcttcat cactaaatgc
      601 agtcaagatt agaattggta aattgctttt ctttctaatt tctttcaaca cttctaaacc
      661 atttataaaa ggtatctgaa tgtctaagat aaccaaattt atatcgctat taaattttga
      721 caaggcttct cttccatctt tagcttgaat aacagtatat ccaaattctg aaagatattc
      781 acttattccc tctcttatca tattatcatc ttcaacagtt aatattttca tatata
//
LOCUS       DARDHA010000324          833 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.336, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000324 DARDHA010000000
VERSION     DARDHA010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.336"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>833
                     /locus_tag="SDA10_002938"
     CDS             <1..>833
                     /locus_tag="SDA10_002938"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEG4241759.1"
                     /translation="SEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSG
                     SLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWP
                     TDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKE
                     TGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKN
                     RQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKEND
                     Y"
BASE COUNT          272 a          149 c          180 g          232 t
ORIGIN      
        1 tcagaagcag ggaagaaagc cggagaattt tacacgcctc atatggtttc tgatatgatg
       61 gcacaaatcg tgacgttgga tcaaaaagaa cgacgattct ttagtgtctt tgacccaacc
      121 atgggttcag gttccttgat gttaaatgta cgtaactact taactcatcc agataacgtt
      181 aaatatcatg ggcaagaact aaatacaacg acatacaatc tagcaaaaat gaacttgatt
      241 ttacatgggg tagatgccga agaaatgaat ctccgcaatg gggatacgtt gaataaggac
      301 tggccaacag atgaacctta cacatttgat gcagtagtga tgaatccacc ttattcggct
      361 aattggtcag cagatacgac cttcttagat gattctcggt ttaatcgcta cgggaaatta
      421 gcgccaaaat ccaaagcaga ttttgcgttt cttcttcatg gtttctatca tttgaaggaa
      481 acagggacta tggctatcgt cttgccacac ggggttttat tccgtggtgc tgcagaaggg
      541 gtcattcgtc aaaaattatt agaagacggc agtatttatg ctgtgatcgg tatgcctgcg
      601 aacttgttct ttggtacatc gattccaacg acggtgattg tattgaagaa gaatcgtcaa
      661 actcgagatg tattattcat tgatgctagt cgtgaatttg ttaaagggaa aaatcagaat
      721 aaattatcgg aagaaaacat tcaaaaaatc ctcgaaacct acgctgaaag aaaagatgtc
      781 gagaaatacg ctcatttggc aacctttgat gaaatcaaag aaaatgacta taa
//
LOCUS       DARDHA010000325          810 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.337, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000325 DARDHA010000000
VERSION     DARDHA010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.337"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            80..793
                     /locus_tag="SDA10_002939"
     CDS             80..793
                     /locus_tag="SDA10_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG4241760.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
BASE COUNT          285 a          105 c          167 g          253 t
ORIGIN      
        1 tataattttg atgtggattt tatcggttta tttatgttaa agtatagatg aagatatgtt
       61 tttgattgga gggtcaccat tgaaaaaaat tgcgaagtat atggaaattt acttagatat
      121 aaagaaaaaa attgtaaatg gagagtataa aattggtgag aaacttccta caggagatga
      181 attggcagtt atttatcata caagcaagtt aacagtaaag aaaggcgtag acttacttgt
      241 ttccgaagga gttttgcgaa gtagaagtgg gtatgggact gaagtcgtca gaacaccaat
      301 tgataactca cgagttcttg gtccaaacga aggtttgttt agcgtggttg gtgaagaaca
      361 cgtagattca gaaatccata ctttctcaat tgaacttcca tcgaaaaaag tagctgataa
      421 attaagtatc agtgtaaaag actatgtata taatattgta cgaagccgtt ttattgattg
      481 tcaaccatac tcgattgagc aaacattcat gccattagca gtcataccag gcttggaacc
      541 aaaacattta aagaaatctg tatatgctta tatcacaaat gaactgggtc tggaaataaa
      601 gacttcgcac atacgtatca aaggagatac tgcgaatgaa tttgacgcaa aaatattaaa
      661 aatagatcca ggtgaattta tgattgagat agacaaagtt gttgctctat cgtctggtaa
      721 acctttcgag tattcggtat cgagacattt atatcaagac tttgtttttg aagctgtttt
      781 tgttgaaaaa taagctcggg aaaattattt
//
LOCUS       DARDHA010000326          797 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.338, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000326 DARDHA010000000
VERSION     DARDHA010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.338"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..156
                     /locus_tag="SDA10_002940"
     CDS             <1..156
                     /locus_tag="SDA10_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEG4241761.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..>797
                     /locus_tag="SDA10_002941"
     CDS             175..>797
                     /locus_tag="SDA10_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG4241762.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSS"
BASE COUNT          355 a           63 c          137 g          242 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctga
//
LOCUS       DARDHA010000327          773 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.339, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000327 DARDHA010000000
VERSION     DARDHA010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.339"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..704
                     /locus_tag="SDA10_002942"
     CDS             <1..704
                     /locus_tag="SDA10_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG4241763.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          263 a          127 c          145 g          238 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atc
//
LOCUS       DARDHA010000328          763 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.340, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000328 DARDHA010000000
VERSION     DARDHA010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.340"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(129..>763)
                     /locus_tag="SDA10_002943"
     CDS             complement(129..>763)
                     /locus_tag="SDA10_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEG4241764.1"
                     /translation="KKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTT
                     AFKIFRAIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYL
                     NFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIID
                     PSMNKWFDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
BASE COUNT          255 a          115 c           90 g          303 t
ORIGIN      
        1 aagctatttt catcgggata ttagttacat taaaagtact aggcataaaa taaagcagac
       61 aaatgattaa atttattact cgaaacgcat ctcgacttaa taaactcttc tctaaaacag
      121 gtactattct attcatctat atttccaact ttcaaaattt ctttatcaat tctatttaat
      181 agtttatcag agtaatccga tttaactttt cttacaaata atttttcact ttcttttatt
      241 tgatctatat ccctttcatc aaaccatttg ttcatggaag gatcaataat atgaatattt
      301 gctaatttcg aagtttgtaa agtagtttct tcaatatttt tcaattcaag tggtaaatct
      361 ggtcctccaa taacattttt atttttgaac gaggaattaa aaaaaagtgt atgaaaaaat
      421 ttttcatctg gggcaaacat acctatttta aaattcaggt agtcttgttg attttgttca
      481 tacatttgta ttaattctgt taggcattct ttcgacaaag cccaccattg agaaccatga
      541 tacaaatcaa atttatggtg tctaattttt gggggaagtt taatcatcct cagacaacga
      601 ttaattatag ctctaaaaat tttaaatgca gttgtgttat tcctattaat tctaggataa
      661 tcatgttttt gatatacatc tatatgttta taataaaatt ctttatgttt aatttgatct
      721 agatctattc cacgaataaa ctccactgaa ctattctttt taa
//
LOCUS       DARDHA010000329          759 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.341, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000329 DARDHA010000000
VERSION     DARDHA010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.341"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>759
                     /locus_tag="SDA10_002944"
     CDS             <1..>759
                     /locus_tag="SDA10_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG4241765.1"
                     /translation="EIEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGY
                     GTSKLLEQRINNGFADMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPL
                     MLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENY
                     AKEGILEGAIKREKLSSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVF
                     FFALNEKVKPELNKIYETFNEIASNHELLANLKT"
BASE COUNT          295 a          101 c          138 g          225 t
ORIGIN      
        1 ggaaatcgaa gagagattct gtatacattt taatgaagat gaaatcgctt acgtaacact
       61 ccatattcaa tcacttttgg atcgttacaa acctgacaaa acgaaagtga tattagtttg
      121 tagtagcgga tatggaacat caaaattatt agagcaaaga ataaataacg gttttgcaga
      181 tatggttgag atagaagatg tactaagcat caatgaacta caagatagaa aagtcactga
      241 tgaacttgtt ataagcacga ttccgattga aaatacaaat tttcctgtta ttgttgttag
      301 tcctttgatg ttaggggcag atgtcagaaa agtaaaacag tatctacagt taaatgaaca
      361 aacagaagat gcttttctaa atttattaga taaacgattt tgctttttta cccaccaaat
      421 aaaggataat caagtgaacg ttttaaatat aattaccaaa aaattggtaa aagaaaatta
      481 cgcgaaagaa gggatactag agggagcaat caaaagagaa aaactatctt caacagcgat
      541 gaatgatttt gctatgccgc atatcgaagt agattttata aaaaagccag taatctgtgt
      601 ctacgttaat ccagaaggaa ttgattggga tgggacactt gttcattctg ttttcttttt
      661 tgcacttaat gaaaaagtaa aacctgaatt gaataaaatt tatgaaacat tcaatgaaat
      721 cgcttcgaat catgagttat tagcaaattt aaaaacgag
//
LOCUS       DARDHA010000330          752 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.342, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000330 DARDHA010000000
VERSION     DARDHA010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.342"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(77..436)
                     /locus_tag="SDA10_002945"
                     /pseudo
     CDS             complement(77..436)
                     /locus_tag="SDA10_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          216 a          138 c          153 g          245 t
ORIGIN      
        1 tgtagtgacc cccaaaagtt agactttttt ggagtggaga aatccactct ttttttcatg
       61 ctctaaaatg atatttttac acaaccgtaa cggttgtttt acgaaattgg acgggacttt
      121 gccagtttaa tttctgtttt atccgttcat tattgtagta atagatatag ctatcgatgg
      181 ctgattttaa ttcctcgaaa cagttataaa ctttcccgtg aaaaatctct tgttttaata
      241 agccaaaaaa attctccata agtgaattat ctaagcagtt ccctttccga gacatgcttt
      301 gaaagatttt gtactccttc aatttgtttc tataagcttt catttgatac gaccaacctt
      361 gatctgagtg aaacgtacga cgatagggac aatcgttagt ggtctggata gcttcctcca
      421 aaccttccat taccgcacgt gtttctgaat ggtggtagcg accgatttga acggctaggg
      481 cggaaatacg gtgcaaatgc acggtggttt catgcaatgc ttacagggcg tattttgtaa
      541 gggtatgtaa cggagataat cccgacggta atggatgtga gaggccaagc cgacgctgac
      601 ggtagggcaa acaaacatag ttgcactcta gcgatcgtta tttattttta acggtcaaac
      661 tagggtacaa ctatgcttaa aacacttcgc ctcaacttct ggtagcaaaa gtgattttga
      721 aagtcaaaat attttttatg agttatttat aa
//
LOCUS       DARDHA010000331          751 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.343, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000331 DARDHA010000000
VERSION     DARDHA010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.343"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            289..687
                     /gene="spxA"
                     /locus_tag="SDA10_002946"
     CDS             289..687
                     /gene="spxA"
                     /locus_tag="SDA10_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEG4241766.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          263 a          107 c          142 g          239 t
ORIGIN      
        1 cggtttcatt tagcttttga gataaaataa tagtacagtt ttatacgctg tttcatatgt
       61 ttagaaaatt tgaattagat ttgaaaactc taatttttaa atgataaaaa aagcgagaat
      121 gctggtttta ctgtatatta accttagata actttcattt ttttaaaaaa attatgaaaa
      181 aaaggtagct atttaaaagt tgattatgta taatagaaac agatgggaga ttattatgat
      241 cttctgttac aaaaaagtga ttcttatcac gaaaaggagt ggtgcacgtt gttaacatta
      301 tatacttccc caagttgtac ttcctgcaga aaggctcgtg cgtggttgca agaacatcag
      361 attccatttg ttgaacgtaa tattttttca gaaccattga acagttctga attaaaagcg
      421 attttacaaa tgacagaaga cggaacagaa gaaatcatct cgacacgttc caaagtattc
      481 caaaaactga acatggattt agatgatctg ccgttacaag aattattgga acttgtgcaa
      541 aacaatcctg gattgcttcg ccgtccaatc atgattgatg ataaacgtct gcaagttggt
      601 tttaatgaag atgaaattcg tcggttccta ccgcgcgatg tacgacaatt agaattacgt
      661 caagcacaat tgatggctgg attatagaag gataatacga aacttggaaa agactggctc
      721 tttgtcaaat gatgttggta aagagaaatc t
//
LOCUS       DARDHA010000332          736 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.344, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000332 DARDHA010000000
VERSION     DARDHA010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.344"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..734
                     /locus_tag="SDA10_002947"
     CDS             <1..734
                     /locus_tag="SDA10_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HEG4241767.1"
                     /translation="DKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRK
                     LMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKI
                     YLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQ
                     YQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIR
                     WYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          318 a           94 c          122 g          202 t
ORIGIN      
        1 cagataaata tagcaagata aagcaagaaa ttacagcgat tgtaaaagaa tcaagaaact
       61 cttatggtta tcgtagagtc actttagcgt taaagatgaa aggatataca atcaatcata
      121 aaacagttag aaaattaatg tcccaaatgg gacttacctg tcaaatccga ataaagcgat
      181 ataaatctta taaaggaaca gtaggaaaaa ttgccaagaa tgtgttaaaa cgaaattttt
      241 cagtagatac acccaataaa aaatgggtga cagacgtcac tgaattcaag ataaaaggaa
      301 gaaaaatcta tctatctcct atacttgatt tatttaacgg agaaataatt agttatagta
      361 tatcaactag tccaacatat aaactgattg aagaaatgct tcaacaagca attaaaaaaa
      421 agggaactga gggttcactg attctacatt cagatcaagg atggcagtat cagatgccac
      481 aatatcaaaa aaaattaaaa gagaataaca ttattcaaag catgtctaga aaaggaaatt
      541 gcttagataa ttctgtaata gaaaattttt ttggtgtgct taaatcagag tttttttatc
      601 gagaaaaatt ccgatcaatt gagatatttc aaagtaaatt aaatgagtat attaggtggt
      661 ataataacaa aaggataaaa ctaaagttaa atggactatc tccagtagaa tatcggaaac
      721 agtccattaa atagtt
//
LOCUS       DARDHA010000333          733 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.345, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000333 DARDHA010000000
VERSION     DARDHA010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.345"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>733
                     /locus_tag="SDA10_002948"
     rRNA            <1..>733
                     /locus_tag="SDA10_002948"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DARDHA010000334          712 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.346, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000334 DARDHA010000000
VERSION     DARDHA010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.346"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..446)
                     /locus_tag="SDA10_002949"
     CDS             complement(<1..446)
                     /locus_tag="SDA10_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4241768.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DARDHA010000335          711 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.347, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000335 DARDHA010000000
VERSION     DARDHA010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..711
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.347"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            169..>711
                     /locus_tag="SDA10_002950"
     CDS             169..>711
                     /locus_tag="SDA10_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG4241769.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLD"
BASE COUNT          283 a           66 c          152 g          210 t
ORIGIN      
        1 ttaaataata tatatgatga aaaagtatgg aacttattaa agaaagatac tttactgttt
       61 aagttgacat ggaaacaatc ttttaaaaag caaaaagatg ataaagttac tttttatggg
      121 atgttggtag aaaacaaatt attataaata ttagaggtat aaaaagttat gaaacacaag
      181 gtaagtgtga tagtaccaat ttacaatgtt gaaaaatatt taagaaaatg tgtggatagt
      241 ttattgagtc agacattaaa agagatagaa atcgtattag ttgatgatgg ttcgccagat
      301 gcttcggggg aaatagcaga tgagtatcaa aaaaaatatt ctaatgtaaa aacaatacat
      361 agagaaaatg gggggttagg accggcaaga aatacaggaa ttgagaatgc gacaggagaa
      421 tatgtggctt ttcttgattc agatgattgg gtagaaagtg atatgtatga aaaattatac
      481 ttagttgcgt ccaaagaaaa tgctgatata gtagttagtg gacattgtga ttttgcgaat
      541 gacaaagcta tggtgaaaaa gcaacatcct ttgagtggga atgtatatga tacgcctaat
      601 agtattaaaa atgtacgaaa aaatttattt ggacatagtc caaaagatca agaagtagag
      661 gccttcccga tgtcagtatg tatgtctttg tataataaaa atctgctaga t
//
LOCUS       DARDHA010000336          693 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.348, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000336 DARDHA010000000
VERSION     DARDHA010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.348"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            132..344
                     /locus_tag="SDA10_002951"
     CDS             132..344
                     /locus_tag="SDA10_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241770.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>693
                     /locus_tag="SDA10_002952"
     CDS             506..>693
                     /locus_tag="SDA10_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HEG4241771.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVL"
BASE COUNT          247 a          103 c          122 g          221 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct aga
//
LOCUS       DARDHA010000337          688 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.349, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000337 DARDHA010000000
VERSION     DARDHA010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.349"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            47..613
                     /locus_tag="SDA10_002953"
                     /pseudo
     CDS             47..613
                     /locus_tag="SDA10_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
BASE COUNT          255 a           96 c          131 g          206 t
ORIGIN      
        1 atgtaagata aaagtgtaaa aacgatttgt tttaggaggg gaaacgatgt gtaccgcaat
       61 cacttatgca acgaaagacc attattttgg aaggaatttt gattatgaga tgtcttataa
      121 tgaagtggtc actattactc caagaaatta tcgtttcgat tttagaaagg taaaaaattt
      181 agataaacat tatgcaatga taggaatagc agctggggta tccaactatc cactctatta
      241 tgaggctaca aatgaaaaag ggttgagtat ggcaggactg aattttcctg gcaatgctga
      301 ttataaagaa ttacaagaag gaaaagataa cgttgcacca tttgagttta ttccatggat
      361 actagggcaa tgctcaacca tagacgaagc taaagaatta ttggctacta tcaatctagt
      421 caatatcgat tttagtgaaa aactaccctt gtcaccttta cactggttat tagctgataa
      481 agaaaaatcg atagtaattg aaagtatgaa agatggactt catttatatg ataatcctgt
      541 cggtgtgttg acaaataatc cgccatttga ttatcaatta tttaatctga acaattatcg
      601 ctcgctatca aatggaacga gagtataaaa tattttgtgt aaatgaaaaa atccatacaa
      661 aaaaggaagt cccttctgta gaataaag
//
LOCUS       DARDHA010000338          688 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.350, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000338 DARDHA010000000
VERSION     DARDHA010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.350"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..626)
                     /locus_tag="SDA10_002954"
     CDS             complement(<1..626)
                     /locus_tag="SDA10_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4241772.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          196 a          138 c          134 g          220 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gatgggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcta tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtctgccca
      361 caatcatagc atagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaatatct gagtacgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccatagag cgactccttt ggtagggtga tgttttggtc
      661 gactaaattt taccaaaagt gagtctct
//
LOCUS       DARDHA010000339          678 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.351, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000339 DARDHA010000000
VERSION     DARDHA010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.351"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..205)
                     /locus_tag="SDA10_002955"
     CDS             complement(<1..205)
                     /locus_tag="SDA10_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241773.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLT"
BASE COUNT          217 a          105 c           91 g          265 t
ORIGIN      
        1 ttgtaagtac tgttggtgtt aatataggga ctttgtacaa ttgtattttt ttgattttat
       61 catctgcaca aaggagattt tttaaatcta atccttcttt tatttcaaaa gttgaatttt
      121 ttaaatctat aagagtaaaa ccaattcctt catactgaat ttctgaaaat cgaatttttc
      181 tcccagttga tagatcaagg ggcagaaaac cttttattac attgtgagtg tcaccatcat
      241 ttacaatgtc attaggaatt ttgattttag aattctgtgg agcacctatt ggaagataat
      301 aattcattat aattttgtag tcttcatgag gaaataaagt acttttagga ttgctaagat
      361 attctcgtac cgaatctaag aatggcttat ccattgaatc ttcatcccca tttaaactaa
      421 aaaacattcc tacaattgct tttgcaactc taagtgaatt cattttcttt attgaatgtg
      481 ttaaatcaga cttaccattc tttaaacttg tattgttata aaggaaattt ttattgtctt
      541 ctgcaaatct aatatattcc tcaacatact cactaccaaa aaaactatta catttactac
      601 ataacgtatt ggaatggcaa cagttttttt gacaaatttt ataaggtgca gaacttcttt
      661 ccgtatgcta ttccgatt
//
LOCUS       DARDHA010000340          676 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.352, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000340 DARDHA010000000
VERSION     DARDHA010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.352"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(267..566)
                     /locus_tag="SDA10_002956"
     CDS             complement(267..566)
                     /locus_tag="SDA10_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG4241774.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          225 a          120 c           72 g          259 t
ORIGIN      
        1 cattttttca atctatattt ccaattttta acttacaaaa atgcaacatt aataaataac
       61 gatagtttca taaaatacac ttttaattaa cattattagt ttatactagc tgtttttgtg
      121 ttaattaaag gataaaatac aatataaaga aacatctgtt tctttattta gaataaaaaa
      181 atacagactc tgttcaaata aatgaaccta gcctgcatta tgagattgac tatacttttt
      241 aattcattga gatgaataac tcgtttttat aaatgcccaa aagtaatcgg tgccgaagcc
      301 attcctcgac ctaattcttt ttcaaatttt cctattgttg atatctccat ataaaacggt
      361 gatactggtt tagacaattc tcccctgatt gctaacggac gtaaactacg ttccaaatta
      421 tttatgactt taggataata ctgacctttt tctaagcctg tctttgcgtc tatcaaaagt
      481 atacgttcct tttctttgat ctctggattt aaaacgagat catataattt tagtaccatg
      541 ttttctattt cttcactttt agacatcacg atttcctccc aaaaatatat ttcttcccac
      601 tacattctat aaacaaagta tacgctacat ctagattttt ttaaatcatt actggattgt
      661 taaaaccttt caatac
//
LOCUS       DARDHA010000341          670 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.353, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000341 DARDHA010000000
VERSION     DARDHA010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.353"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            62..247
                     /locus_tag="SDA10_002957"
     CDS             62..247
                     /locus_tag="SDA10_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG4241775.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            259..507
                     /locus_tag="SDA10_002958"
     CDS             259..507
                     /locus_tag="SDA10_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241776.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
BASE COUNT          244 a           96 c          140 g          190 t
ORIGIN      
        1 tcaacgaata ttagctaatt ttttagctta cagattattt ttttagaaag gagctatttt
       61 tatgtctgag aatttagact taaaaattcg agcggagatg agaaaaagaa gaatgacttt
      121 caaagaacta gctgcgctta tcggtatttc aggagcttat ttatcagata ttctaaacgg
      181 caatcgtgat ggaaagaaag cacaacagca tatcgaaaca gtgaaaaaaa tattggacat
      241 ccgatagggg ggtaggcgat gacaaaacta aaaaaacaag attttgtaaa aaaatacaat
      301 tattctccat ctacttatca acgtcgaatg tcggaactaa aaaatacagc aattttctca
      361 gcggcgtatg aacgggtcac aggacaagaa gtttggatca atacagaatt atacgataaa
      421 tttttgtctt tcaaatccta taacaggtta cgcacaagaa aggtaacacc taaagaattt
      481 atcgagaagc atttagttga tttataaaaa acagaacatt ttgagaggtg aaggttaatg
      541 ggtaaattca acagagcatt ggtattcagc gcaccgctaa tcatctacgc tttaggactt
      601 tggggaagca ggcaagcgtt gataggaacg attgtttaca tggtttggat ttttatgggg
      661 cttgatgaag
//
LOCUS       DARDHA010000342          652 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.354, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000342 DARDHA010000000
VERSION     DARDHA010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.354"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(101..>652)
                     /locus_tag="SDA10_002959"
     CDS             complement(101..>652)
                     /locus_tag="SDA10_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HEG4241777.1"
                     /translation="DRTFTQEQPLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYS
                     CGEKKDASLVKEAFGRIPYSLTDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGC
                     PYDNAVVESTYKSVKVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQI
                     RQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          183 a          151 c          123 g          195 t
ORIGIN      
        1 ttataaggtg ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc
       61 ctcggatctg attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg
      121 tatcagatcc gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga
      181 tcggtgtttc atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat
      241 caaacaattc tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta
      301 ctttgacaga tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac
      361 tcaatgaacg agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt
      421 cctttccccg gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa
      481 aggcttcttt taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc
      541 gattaaacaa atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag
      601 taagatccgt aacgatggct tccaatggct gttcttgtgt aaaggtccga tc
//
LOCUS       DARDHA010000343          636 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.355, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000343 DARDHA010000000
VERSION     DARDHA010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.355"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(195..344)
                     /locus_tag="SDA10_002960"
     CDS             complement(195..344)
                     /locus_tag="SDA10_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEG4241778.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            complement(337..546)
                     /locus_tag="SDA10_002961"
     CDS             complement(337..546)
                     /locus_tag="SDA10_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG4241779.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
BASE COUNT          190 a          125 c           63 g          258 t
ORIGIN      
        1 cacttttgaa tgtcaattgg acgtatttct cttaactcca ttttaaagaa ataagaaaag
       61 tttttttgat tgtagataac cgatttttat atgttctttc ttctactttc ccttgataat
      121 aaggtaggaa taatttataa aaaaactcct caaacaacat agtactttga gaagtttctg
      181 cttcttttcc ataattagaa acaagttcgg tatacgcttt ttttcttctt taactgtttt
      241 aacccctcta cgtttttttg tactcttttc cctgttcgtt tgtcgaatcc caaactagca
      301 gtaaaatgat atttcccatt tttatctttt ttaaaattaa ccatgattct gaaaatcctc
      361 ctctacctct atcttcatac ctaaaacttc ttctacagat tcaacaggta ctagtcctaa
      421 tcttctattt tcgtaatagg tatagctttt tgaaaccata attgcttttg cctgtcgtat
      481 gattctctca gactgatact tcccataacc taatttcatt aattcatttt ttgttataac
      541 caccatttaa ccaccacatt ttttattcaa atataaaaaa gaacactact aaaaatagta
      601 gcgttcctgc atatttttat tcaaatttaa tcagct
//
LOCUS       DARDHA010000344          625 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.356, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000344 DARDHA010000000
VERSION     DARDHA010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.356"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            54..542
                     /locus_tag="SDA10_002962"
     CDS             54..542
                     /locus_tag="SDA10_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG4241780.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
BASE COUNT          256 a           51 c          113 g          205 t
ORIGIN      
        1 atgtgaaaac atgagcaata gaattgtact agtaggataa ggatggatgg aaaatgaaaa
       61 aaactatttt tgttttagcg atttttatat gttttgggct acttttggta ggctacttgt
      121 tgtttaattc taatgaaatg agaactattt ctacgaatga cttgttaaaa aaaagtgaat
      181 taaaggataa aaaaaaagaa tctcttgcct ttataatgtt tactcaagaa gagtgtgagt
      241 attgtatagc aataaatcca atcatagata gtgtaaataa ggaaactttg aataagataa
      301 agaaatatga tatatctgta gattttcaat ctaaacaaaa gtgtaaaatt aagtttgttc
      361 cgactattgt tgcatatagt aagaataaag aagtggggag aattgacaat gaattagata
      421 atttgtatcg aaaaaagcaa gatggttcaa ttgtaataga caagaaaaaa acaaagacat
      481 tgcttataga ttggatgaaa aaaatggata aacaaggaga atatttgtat ggagaagatt
      541 aaacagacat ttccgttaat taataaatat tggaataaga aaaaaatttt tatatttttt
      601 attacgtttc tgctgtcatc tttat
//
LOCUS       DARDHA010000345          608 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.357, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000345 DARDHA010000000
VERSION     DARDHA010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.357"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          200 a          119 c           85 g          204 t
ORIGIN      
        1 gaaccggggg ctttattcct gcagtccggc tacgccttcc tgcaatagac aaaactccag
       61 atcgggtaat tcgcttgtgg cgaaacccga cctggagttt gtctctgtat tttgaaccct
      121 aaaaattaat caaacagact gatattctca taatcagcgc gaaataagcc attaagtaca
      181 gttttcctac ctatttttga taacccgctg gttagttaat attccttttc cgagagagat
      241 aaatgattgg ttaaatctat atacatctca acctttaaaa atgttttcga aatacttcat
      301 cagtacatgt catacaaatt accagtgcat tctcaaattc tagttcttta attgatttca
      361 gttgaggagc agttactttt ggcggaaatt tccatttttg tccaaacctc ttcccccgat
      421 gaaatttctt tgttgcaatt attgcaatga aaaatttttg tttcaaaaat atacataatt
      481 acttcctcca ataaaaacta tctacctctc aaattatttt tctctaataa gaaaaacaga
      541 aaaggtaata agatacaaca gtgatgattc ctaataaaga aaagactaca ataatatttt
      601 tctaactt
//
LOCUS       DARDHA010000346          607 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.358, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000346 DARDHA010000000
VERSION     DARDHA010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..607
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.358"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(161..418)
                     /locus_tag="SDA10_002963"
     CDS             complement(161..418)
                     /locus_tag="SDA10_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HEG4241781.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
BASE COUNT          182 a          107 c           88 g          230 t
ORIGIN      
        1 aagcaaaaaa ataaagaaac ttattcttga taacaagcta cgtgacgtat atccctaaca
       61 caaaaatagc catatccttt ggtttggata cggctatttg atattactca ttcaattatc
      121 aaatcacagt aggtgtgaat ctcatcggga tcggcaatat ctacttctga ttaggatcaa
      181 aggtgatttt ctcaataaag gtgtgataca aagctttaac ttgcttcttc tcactctgga
      241 taagaaagca atccaaacta gtcaatatct gttggacatc tttgacttga atcggttccc
      301 cttgttgttg aaagatccct aagatttcat tctctcgaat ttgtaactgt cttcgttttt
      361 cagtcaattc atttaaacga tcttttaaca tcggaaacaa ttcgggacta tcttccaaag
      421 cagcttcctg tttttgctct aatagttaga ttttgcgtac cattttcttc ttttattttc
      481 cttcgattgc tgattttaat atagtctaaa ggaaatttat atttattgta ttctttataa
      541 cttcaagaga acttcgtttg agtattactg tattttttta attccataag aaaattagtt
      601 attacct
//
LOCUS       DARDHA010000347          600 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.359, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000347 DARDHA010000000
VERSION     DARDHA010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.359"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..435
                     /locus_tag="SDA10_002964"
     CDS             <1..435
                     /locus_tag="SDA10_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEG4241782.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT          195 a          134 c          112 g          159 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
//
LOCUS       DARDHA010000348          595 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.360, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000348 DARDHA010000000
VERSION     DARDHA010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.360"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1..563)
                     /locus_tag="SDA10_002965"
                     /pseudo
     CDS             complement(1..563)
                     /locus_tag="SDA10_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          168 a          109 c           78 g          240 t
ORIGIN      
        1 taattgtgca attgaaagta atagattcaa tttaaattct tttttgagct gaaggattgt
       61 tttaaccttg atttcttctt gctcaaatct tgttcttgaa tcaaggtttc taactttttt
      121 aaatatgcgt tctctgctct taaacgaata acttcttctt caagagactc atctttaagt
      181 tttttaggaa tgtttagctt ggaattcata ctaatttttc tgcccctttt ttggctctca
      241 agtgaagaag caccgccttc ttcatattgc tctatccatt tacttagagt tctatttgaa
      301 ccgatattaa attttttagc agtttcttgg atagaaagac catttgtttc catatattct
      361 ataacatcaa gtttaaattt tgtagtgtag cttttgccac ctccaaccaa gccttcccaa
      421 ccatgatagt tataaatcct tacccaatgt ctaaccagtg tacgatttat ttgatattta
      481 tgtgcaagat atttgtagcc gccttcgtta tttaaatagt ctgaaactac ttttttctta
      541 aaaacaaatg tatatttccg caaaaaaagc acccctttta attagatttc tagtc
//
LOCUS       DARDHA010000349          593 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.361, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000349 DARDHA010000000
VERSION     DARDHA010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.361"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>593
                     /locus_tag="SDA10_002966"
     CDS             <1..>593
                     /locus_tag="SDA10_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEG4241783.1"
                     /translation="SVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFF
                     DKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVI
                     WMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDY
                     YSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIA"
BASE COUNT          184 a           75 c          115 g          219 t
ORIGIN      
        1 ctctgtagct ttgatgcaga cattatattt tgatgataaa tatgttggca gagtagtcgg
       61 cggaacactg tcatttgtgt tgataggttt agtaatttat ttaagaataa tgataaaggg
      121 tgctcatttt tttgataaaa aacagtggag atacgcttta aaaatctctt tgccgctaat
      181 cccacattcc ttagcaaata tagttttagc acaattcgat agaattatga ttaattctta
      241 tttaggtcct gctgcggggg gaatatattc ctacattagt aacataggta ttattctaag
      301 cgtaatatgg atgtctacta ataacgcctg ggttccatgg ttctatggtg agatgagtaa
      361 aaataatgaa aaaaaagtga agaaagttag taactattat ttagtagtct ttactttgat
      421 tacgatgatt gtaatggtag tttcgattga tttagcaaaa tttatggctc cgaaagacta
      481 ttattctggg ttatctttag tggttccgat aaccttaggc tatttctttc agtttttata
      541 tagtttacca gttaatgctg aattttatga gaaaaaaaca tcgtttattg cga
//
LOCUS       DARDHA010000350          582 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.362, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000350 DARDHA010000000
VERSION     DARDHA010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.362"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>582
                     /locus_tag="SDA10_002967"
     CDS             <1..>582
                     /locus_tag="SDA10_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEG4241784.1"
                     /translation="QDTRELQYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSV
                     EELEMLTSHTFQSKTLHGCYRSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCK
                     VTKEDLPNIIKNIIKEKIESGIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINI
                     SPIKVNNHSLFFKLAILYFTESGEKVRRRKLIA"
BASE COUNT          220 a           84 c          100 g          178 t
ORIGIN      
        1 tcaagatact agagaattac aatacaatgt aatagctaaa ttacttcaaa acaaccctca
       61 aattcagtca atgtttgttg gagatattga ccaagcaatt tatggatcat tagatggtat
      121 agctaaatca gttgaagaac tagaaatgct aactagtcac acttttcaat caaagacgct
      181 acatggctgt tacaggtcta atcagagatt agtagacttc tactctaatt ttcagagttg
      241 tccttacgag attgaaagca gagggaataa taaaaacgac cgtggtttta taagttatga
      301 ttgcaaagtt actaaggagg atttacccaa cataataaaa aatataataa aggaaaagat
      361 tgaaagcggc attcatgaaa aagatatttg tgtacttgct cctcaacact atcctctgtt
      421 ttcactgggt gagtatttaa agaaagaatt accgtattgc aattttgatg ctataaatat
      481 atcaccaatt aaggtaaata atcatagttt atttttcaag ttagcaattt tgtactttac
      541 tgaatcaggt gaaaaggttc gaagaagaaa gctaattgca aa
//
LOCUS       DARDHA010000351          577 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.363, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000351 DARDHA010000000
VERSION     DARDHA010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.363"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>577
                     /locus_tag="SDA10_002968"
     CDS             <1..>577
                     /locus_tag="SDA10_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241785.1"
                     /translation="MEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLK
                     RIELLALSGAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNL
                     GITDDGARLSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHS
                     DILTIYIEVGCIVFIIWMFWLFFVKPIRLTKK"
BASE COUNT          182 a           57 c          109 g          229 t
ORIGIN      
        1 tatggaggta catgatttaa cttttgcgat gggggttttt ttcctttatt acgtattttt
       61 tgaaaataaa aatacaaaaa aacataagat aaaaattatc gtatctttaa ttttaatttt
      121 catgggattg aagagaattg aactgttagc tttaagtggt gcaattctag tgtattatgt
      181 tttattaaag tggggaaaaa agattcaaat ccgtgcatta gtgtgtacta tttgtgcaac
      241 tgttatttcg ttagtgttta tttacattat agatacagga attttggcat tgcttatgtc
      301 aaatttgggc attacagatg acggtgcgag gctttcattt tatctatatt catctaaatt
      361 ttatgaactt ggtttaggat atgtaggaac aggatggaca tggttttcaa aatacttttt
      421 taatctttat ttatcgcact ttaggttaaa tggtggaaga attgcagcaa gcctacacag
      481 tgatatatta actatttata tagaagttgg atgtatagtt tttattatat ggatgttttg
      541 gttatttttt gtgaaaccga taagactaac taaaaaa
//
LOCUS       DARDHA010000352          569 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.364, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000352 DARDHA010000000
VERSION     DARDHA010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.364"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            28..>569
                     /locus_tag="SDA10_002969"
     CDS             28..>569
                     /locus_tag="SDA10_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HEG4241786.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYR"
BASE COUNT          205 a           92 c          114 g          158 t
ORIGIN      
        1 tagaaaaaga tagcaggagg aatgacgatg aatcatttta aaggaaagca atttcagcag
       61 gatgtgatta ttgtagccgt gggctactat cttcgttata accttagcta tcgtgaagtt
      121 caagaaatct tatatgatcg tggcattaac gtttctcata cgacgattta tcgttgggtg
      181 caagaatatg gcaaactact ctatcaaatt tggaaaaaga aaaataaaaa atccttttat
      241 tcatggaaaa tggatgaaac gtacatcaaa attaaaggaa aatggcatta tttgtatcga
      301 gccatcgatg cagatggttt aaccttggat atttggttac gtaaaaaacg ggacacacaa
      361 gcagcctatg cttttcttaa gcggttagtg aagcagtttg atgaaccgaa ggttgtagtc
      421 acagataaag ccccctctat tacaagtgcc tttaagaaac taaaagaata cggcttttat
      481 caagggacag aacatcgtac cattaaatac ctgaataatt tgattgaaca agaccatcgt
      541 ccagtaaaga gacgcaataa attctatcg
//
LOCUS       DARDHA010000353          565 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.365, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000353 DARDHA010000000
VERSION     DARDHA010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.365"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..561
                     /locus_tag="SDA10_002970"
     CDS             <1..561
                     /locus_tag="SDA10_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG4241787.1"
                     /translation="YQYHVGQYLPDWHPWEDYRNFFVSNKKTNGCREIFAIELPWIID
                     TFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETGIVGTLSVDVVSRVAKRNLTIIGE
                     KILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHDNNYSHTIIEDAYLEEIKEFFTLL
                     EKKMEPQYTFEKDKYIINLINDIEVN"
BASE COUNT          204 a           67 c          102 g          192 t
ORIGIN      
        1 tatcaatacc atgtaggaca gtatttaccc gactggcatc cttgggaaga ttatcgcaat
       61 ttttttgttt caaacaaaaa aactaatggg tgtagagaaa tattcgctat tgaattacct
      121 tggattatag atacatttgg gaaagttaaa tcgtttactg taatgaccca gaaattatcc
      181 aatcttgata ttgattattc ggacagtttc actcttatta ttgagcatga aactggtata
      241 gttggaactt taagtgtaga tgttgtttca agagtagcta aaagaaattt aactataatt
      301 ggtgaaaaga ttttaataga atgggaaggt aacccggatt ctctattttc atggaatgaa
      361 gaaacttcta gtatgacaga tattcaattg tacgatgagg ttatgcatga taataattat
      421 tcacatacta ttattgaaga tgcatattta gaggagatta aagaattttt cacgcttttg
      481 gaaaagaaaa tggaacccca atatactttt gaaaaagata agtatattat taacttaatt
      541 aacgacattg aggtaaatta atgat
//
LOCUS       DARDHA010000354          554 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.366, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000354 DARDHA010000000
VERSION     DARDHA010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.366"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            39..>554
                     /locus_tag="SDA10_002971"
     CDS             39..>554
                     /locus_tag="SDA10_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241788.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTY"
BASE COUNT          202 a           78 c          112 g          162 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgagaacctt gggttttcaa gtgctttttg
       61 gttcattaat cttaaccata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
      121 ggagccttgt aggaatagtt cttattattt ttactgtgac tgcagcaaca gaagcaaatt
      181 attcgcataa aattcagtcg tataaaggtc gtttagttat gaccaaaaaa gatgttacac
      241 gttatacatc ctcaagaact ggaggcctat atgactatgc accattagat gcattgaaat
      301 ataaagatca tttaaagaaa catgaagtaa aagttggtga tcggtgggag aatgcatctc
      361 ataaagcata taattccaaa atagtctata cagtagaaga tgccgatgag caaaatatca
      421 cattacctgt attcaattat tggggaacag ttgtgaaagt aaatgggaaa aagagcacta
      481 tagaaaatga caatggttta gtgtctatta aaggtacggg gaaaagaact atagttgagg
      541 tttcatctac gtat
//
LOCUS       DARDHA010000355          527 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.367, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000355 DARDHA010000000
VERSION     DARDHA010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.367"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..515
                     /locus_tag="SDA10_002972"
     CDS             <1..515
                     /locus_tag="SDA10_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241789.1"
                     /translation="TAGSRTALQLSDIFNPMIDIYKEKKYIFFTSVYDFEGGEPVGEY
                     ELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSSIPWEAIQLSGDFNDKIFM
                     TINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAYDIERLLKQKTMKNILKNV
                     KIAESLDDIL"
BASE COUNT          205 a           64 c          101 g          157 t
ORIGIN      
        1 ttacagctgg gtcaagaacc gcattgcaac tttcggatat ttttaaccct atgatagata
       61 tttataaaga aaaaaaatat atatttttta cgtctgttta tgattttgag ggcggagaac
      121 ctgtcggaga atatgaatta gtatgcaaaa tagcagactt agtaggaaaa gataatctaa
      181 ttgttaagac gcatcctaga gatgtaagaa caatttataa agataatgga tttatcgagg
      241 ataaaaattc atcaatacct tgggaggcga ttcaattgtc aggcgatttt aacgataaaa
      301 tatttatgac aattaattca ggaagtgtta tttcaggtag tacaatgtct gcaaaacctg
      361 tacgcacgta ttatatgtat aaattgtgta acatagaagg aaatccgtct tgcaaaaaga
      421 atgcgtatga tattgagcga ttgcttaagc aaaaaacgat gaaaaacatt ttaaagaatg
      481 tgaaaatagc agaaagctta gatgatatac tatgagcaaa actaaat
//
LOCUS       DARDHA010000356          526 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.368, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000356 DARDHA010000000
VERSION     DARDHA010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.368"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="SDA10_002973"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="SDA10_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEG4241790.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(349..>526)
                     /locus_tag="SDA10_002974"
     CDS             complement(349..>526)
                     /locus_tag="SDA10_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241791.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DARDHA010000357          522 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.369, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000357 DARDHA010000000
VERSION     DARDHA010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.369"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          186 a           78 c           99 g          159 t
ORIGIN      
        1 ttgatgacca cgaaagtgca tggcgatatt tgatgttgat actttatgca gtaccctatg
       61 tattcttcgc tatcattctg tggctcatag cattgttttt tagagtattt aatgaccttt
      121 aggtagattt atatataaaa agggctaaat acgcaaaata cccccttaaa atgcaaaata
      181 aatgcaaata agggggtaac tccttatgaa aaaacaacta atagtattat aacacgtttt
      241 tatgccgaga aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt
      301 ggttggactt gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc
      361 tgtaggttcc gaccaataag gaaattggaa taaagcaacg aaaggagttg agtaaaatga
      421 gtgagaaaac gaagaaaatc attcgagtat cattagcttt ttcacaagta gtagtgcaat
      481 cattgatagt ttattatact taccagcaat ataaattatc ta
//
LOCUS       DARDHA010000358          514 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.370, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000358 DARDHA010000000
VERSION     DARDHA010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.370"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..132)
                     /locus_tag="SDA10_002975"
     CDS             complement(<1..132)
                     /locus_tag="SDA10_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEG4241792.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DARDHA010000359          499 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.371, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000359 DARDHA010000000
VERSION     DARDHA010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.371"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>499)
                     /locus_tag="SDA10_002976"
     CDS             complement(<1..>499)
                     /locus_tag="SDA10_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241793.1"
                     /translation="MVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEA
                     VALTFLPLILMGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFY
                     FWDQKIARLLSLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTH
                     ILNNN"
BASE COUNT          193 a           85 c           84 g          137 t
ORIGIN      
        1 tagttattat ttaaaatatg tgtcagcata tcactaggag ccataccact taattctttt
       61 cccagtggga ctttcaattc ttgtgctcta gattgttgaa taaaaggaat taagaatccc
      121 gcacttaaaa aaaaggtgag gacggtagct tttaaaagac ttaagagtct agcgattttt
      181 tggtcccaaa aataaaaaga aagaaacaac gttcctccaa taaatacaga aaccatagcg
      241 acagaaagta aatgagtata cactactagc gtcatgccaa tagacaacca gtaccatttt
      301 tggtaatctc taatataaat ttcgtaacac cccattagta tcaaaggcaa aaatgttaaa
      361 gcgacagctt ctcccaatga agcacgatgg aatatgtcaa tggcacgata ggcggaaaat
      421 gtataaataa tcgaaaataa cagcgaaata taacgattgt tttttatttg tttcatagaa
      481 aaataagaaa cgaccattg
//
LOCUS       DARDHA010000360          493 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.372, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000360 DARDHA010000000
VERSION     DARDHA010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.372"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            107..463
                     /locus_tag="SDA10_002977"
     CDS             107..463
                     /locus_tag="SDA10_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG4241794.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT          193 a           74 c           99 g          127 t
ORIGIN      
        1 agcaataaga acaaaatgag gaaaagttta aaattcagct gttgccaaca actttcaata
       61 gacgaaatct tctatttcgt taataataag gaaagaggtg aagtacatga aaaaattaat
      121 tggcgtactt gtcgttttag gcatatttct cggcggaagt tttgcagctt atcattattt
      181 ttacggcggc gaagcttatt atacaaaaat cacgacgaat ggcgaaatgt cagctaccca
      241 agcagataac ggagagaaat tcacgactta tacctacaag caagcggctt ataataaaga
      301 tggtgaaaaa aaacaggtga cgcttcgcga agaaagagaa catccattaa agatgaatgc
      361 gtatcttaaa ctcaaagtca atcctcgtaa aggtgtatta agctgggaag aaataaaggc
      421 ttctgaagtg cctaaaaatg cggcagaaaa aatcgataat tagtaaatgg tcttaaaatt
      481 ttattagata aaa
//
LOCUS       DARDHA010000361          487 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.373, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000361 DARDHA010000000
VERSION     DARDHA010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.373"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..368)
                     /locus_tag="SDA10_002978"
     CDS             complement(<1..368)
                     /locus_tag="SDA10_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241795.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGL"
     gene            complement(385..>487)
                     /locus_tag="SDA10_002979"
     CDS             complement(385..>487)
                     /locus_tag="SDA10_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEG4241796.1"
                     /translation="SDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          174 a           93 c           47 g          173 t
ORIGIN      
        1 agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa tttttgataa
       61 tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc atctagctta
      121 atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat agattctcta
      181 gcttcttcac ataaactttt tactttatta attactactt tgtcatattc atttaacaca
      241 ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc agaaatttcc
      301 caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg taaatgatat
      361 agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca ctaaacctaa
      421 ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa agtgtgaaga
      481 atctgaa
//
LOCUS       DARDHA010000362          485 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.374, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000362 DARDHA010000000
VERSION     DARDHA010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.374"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(62..259)
                     /locus_tag="SDA10_002980"
     CDS             complement(62..259)
                     /locus_tag="SDA10_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG4241797.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DARDHA010000363          472 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.375, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000363 DARDHA010000000
VERSION     DARDHA010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.375"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            23..>472
                     /locus_tag="SDA10_002981"
     CDS             23..>472
                     /locus_tag="SDA10_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241798.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLIS"
BASE COUNT          145 a           46 c           83 g          198 t
ORIGIN      
        1 tatatgtaga cggggcgaaa cgatgagtgt aaaaaaaatg ctatttatgg taagtacaat
       61 aattgtatta acatcccgat tttggggaat taatactagt ttatctatgc gatattttat
      121 tatggcaata tgggatttat attttatgtt aagctatttt tcttatcgtg agaaaacata
      181 taaaaaatac gttatcaaga ataacttttg gttgactata atgccgtttg tgatattttc
      241 tatctatact ctaataatat ggggaattgg ttcaaatgca gaatttgaaa attttataaa
      301 gttatgttcc acgttgcttt atttaattct tgcatatggt tacgcatgta cagcatttta
      361 cttatttaag gcagaaggaa ttgattttat tttttggtca ggtgttattt catatatgag
      421 tggttcagtt ttttatttaa ttgtatctta tggtttaaat ggtttaattt ct
//
LOCUS       DARDHA010000364          457 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.377, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000364 DARDHA010000000
VERSION     DARDHA010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.377"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            114..>457
                     /locus_tag="SDA10_002982"
     CDS             114..>457
                     /locus_tag="SDA10_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEG4241799.1"
                     /translation="MDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDL
                     TQYNYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNY
                     SAFKSMGGGVEL"
BASE COUNT          175 a           49 c           77 g          156 t
ORIGIN      
        1 aaaatctaat aagctatgga aaaaagaatt ctattaattt tgatataaca gcttatagta
       61 gcagtggaaa aaaagagttt gaagaaataa cgtatattca aaatagagac gatttggata
      121 cacattatga tatagctttt attacaaatc cgacttttaa gcatctggaa acactaaatg
      181 taattgttga aaaagttgat tacatatttt tagaaaaacc tgctttttct gagagtattg
      241 atcttactca atataattat gattacaaaa atatttatgt tgctgctcca ctgaggtata
      301 aaaatatcat ggaagttgtt cgtaatagta ttcaaagtaa aaaagtatat tctgtaagag
      361 ctatttgttc tacttatctt cctgattgga gaaaaggtac tgattataga aataattatt
      421 ctgcctttaa atctatgggg ggaggagtag aattaga
//
LOCUS       DARDHA010000365          452 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.378, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000365 DARDHA010000000
VERSION     DARDHA010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.378"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>452
                     /locus_tag="SDA10_002983"
     CDS             <1..>452
                     /locus_tag="SDA10_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241800.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGST"
BASE COUNT          165 a           68 c           92 g          127 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca ac
//
LOCUS       DARDHA010000366          447 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.379, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000366 DARDHA010000000
VERSION     DARDHA010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.379"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..425
                     /locus_tag="SDA10_002984"
     CDS             <1..425
                     /locus_tag="SDA10_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG4241801.1"
                     /translation="EYTDYCYRKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLE
                     CQIRAKRMAIDYCRLYVGLVDDANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQ
                     QKFFHKMLECKCYTILLLLSRIRKIIKNMTRKKLFIIL"
BASE COUNT          196 a           40 c           74 g          137 t
ORIGIN      
        1 cagaatatac agattattgt tatagaaaag aagaacaaga gtctattaca aaaagtttat
       61 ctccaaataa gcagacggaa tttaaagaat ttttaataac tctaaaagct atagcatcga
      121 aagaagattt agaatgtcaa atcagagcaa aacgtatggc aattgattat tgtaggcttt
      181 atgtgggctt agttgatgat gcaaatttgg gaattaaaga gaaaagaaag tatataaaaa
      241 aatttattga agatgaggga ttaattcaat gttggaaggg atatcctaca aatacgctac
      301 catttcaaca aaaatttttt cataaaatgc ttgaatgtaa gtgttataca atattgttgt
      361 tgttaagtag aattagaaag ataattaaaa atatgaccag aaaaaaattg tttataatat
      421 tataaactaa aagtatatca aataata
//
LOCUS       DARDHA010000367          441 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.380, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000367 DARDHA010000000
VERSION     DARDHA010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.380"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            20..334
                     /locus_tag="SDA10_002985"
     CDS             20..334
                     /locus_tag="SDA10_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241802.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          142 a           55 c           71 g          173 t
ORIGIN      
        1 gttgctagga ggatagcttt tggattttta cagtaatttt atcttaataa tagcaatatt
       61 attacttcta aatatttggt tttttgataa gagtagaaat gcagggatag gttttagaac
      121 caaacgaagt acgtcttctg aaaaaaagtg ggtatattca cagactattt tttacggagg
      181 ggttatttca atcagtttac tttcttcaac actttattct tttaatgtta tagacgtttc
      241 catgtcaaat tttatatcta ttattggaat ccttatttct gcaattatta cacaactact
      301 tcttgtgttt gaagagaagt caaaaaataa ttaaagcaat tatgcctttt agtacaggaa
      361 ttattacttc ttacaaatgt atagcgaatg tgaagcctgc tgttttaatt gttctagaaa
      421 aacttttgag tgaacaatta t
//
LOCUS       DARDHA010000368          426 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.381, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000368 DARDHA010000000
VERSION     DARDHA010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.381"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            22..>426
                     /locus_tag="SDA10_002986"
     CDS             22..>426
                     /locus_tag="SDA10_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HEG4241803.1"
                     /translation="MIESYYLQHNKPVEIANRMGRAIQTIYNVVNKFKQGKTALDYWH
                     QYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVSCGMRTLYRLFSK
                     GIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIH"
BASE COUNT          160 a           74 c           83 g          109 t
ORIGIN      
        1 cttaccatag acgaactgac aatgatagaa tcatattatc ttcaacataa taagccggtt
       61 gaaatcgcta accgaatggg acgtgctata caaactattt ataatgtagt caataagttc
      121 aagcaaggca agactgctct tgattattgg caccagtata aagaaaataa gaaaaaatgt
      181 ggtagaaaag tcattcaatt acctgctcat gaagtagatt acattaaaga gaaagtcact
      241 cttggttgga cgcctgacgt cattatcggg cgaaaagaaa ggtctgtttc atgcggtatg
      301 agaacacttt atcgtttatt ttctaaagga atatttgata ttgacacact accgatgaaa
      361 ggtaaaagaa aacccaatgg ccatcaggaa aaacggggaa aacaacagta tcagcgctca
      421 atccat
//
LOCUS       DARDHA010000369          421 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.382, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000369 DARDHA010000000
VERSION     DARDHA010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.382"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(80..152)
                     /locus_tag="SDA10_002987"
     tRNA            complement(80..152)
                     /locus_tag="SDA10_002987"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(117..119),aa:His,seq:gtg)
     gene            complement(214..298)
                     /locus_tag="SDA10_002988"
     tRNA            complement(214..298)
                     /locus_tag="SDA10_002988"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Leu,seq:caa)
     gene            complement(319..389)
                     /locus_tag="SDA10_002989"
     tRNA            complement(319..389)
                     /locus_tag="SDA10_002989"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(355..357),aa:Cys,seq:gca)
BASE COUNT          121 a          106 c           96 g           98 t
ORIGIN      
        1 ggggtagctg gattcgaacc aacgcatgag ggagtcaaag tcccttgcct taccgcttgg
       61 ctatacccca agaataaagg gcgaatgatg ggaatcgaac ccacgaatgc cggagccaca
      121 atccggtgcg ttaaccactt cgccacattc gccatcataa aagatgttat ttaatttctc
      181 aataaaaaac taagatgaag acatcttagt caataccggc ggccggggtc gaaccggcac
      241 gccctcaacg ggcactggat tttgagtcca gcgcgtctgc caattccgcc acgccggcaa
      301 gttgatttat tagtactaag gcggtaaccg gatttgaacc ggtgatgaag gttttgcaga
      361 cctctgcctt accacttggc tataccgcca ttatactatt atcaatctat aaaataaaga
      421 a
//
LOCUS       DARDHA010000370          417 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.383, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000370 DARDHA010000000
VERSION     DARDHA010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.383"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>417)
                     /locus_tag="SDA10_002990"
     CDS             complement(<1..>417)
                     /locus_tag="SDA10_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEG4241804.1"
                     /translation="EIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEE
                     SLESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQ
                     FIFELIFISIPSIISSLFLGNVLLKVIVDGFINSED"
BASE COUNT          153 a           72 c           39 g          153 t
ORIGIN      
        1 aatcctctga gttaatgaat ccatctacaa ttacttttag taatacattt cctaaaaata
       61 aggaagatat tatacttggt atcgatatga atattaactc gaatataaat tgcattataa
      121 tttgtatctt agatgttcca atagataaga atatacctat ttcataaatt ctttccctta
      181 accatagaat caagattaat gaaagaacaa ccatcccacc taacataata gaataagtca
      241 ttacttttat tatatatttt attccactca ctgactctaa agactcttca aacgcattag
      301 aatctttttc aacaaaatac tttgactcat caattttaag ctctttcaat ttgtttaagg
      361 ctaagtctgt agattctgca ctaccagaat acattaaaat tttatttgca atttcat
//
LOCUS       DARDHA010000371          415 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.384, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000371 DARDHA010000000
VERSION     DARDHA010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.384"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..339)
                     /locus_tag="SDA10_002991"
     CDS             complement(<1..339)
                     /locus_tag="SDA10_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4241805.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DARDHA010000372          409 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.385, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000372 DARDHA010000000
VERSION     DARDHA010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.385"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>409
                     /locus_tag="SDA10_002992"
     CDS             <1..>409
                     /locus_tag="SDA10_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEG4241806.1"
                     /translation="EQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFN
                     LSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVS
                     LPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSV"
BASE COUNT          154 a           49 c           74 g          132 t
ORIGIN      
        1 tcgagcaaat caaaatagat aatctgttat cacaaaatta cgatccagaa atatatctgt
       61 ttctttatga aaataaaata ctggaatatg ttaaaaatgg agatattcga aatttagaaa
      121 acatggtttt taatctgagt aatggaataa ttccaagtgt tagtggagat acgattcggt
      181 ctgaaaaaaa ttattctatc attgtttttg aaaagttagc acaaacgtct ataacattag
      241 gaatggatat aatagaagcg tatcaaagta gagatgcttt gatacaagaa aacgaattag
      301 ctgtatcgtt acctgaggtc ttaaaagtga gggattctgg gattgtttat tatactaagg
      361 aaatcggaaa aactaagata gagcatcttt ctcccttaat ttcttctgt
//
LOCUS       DARDHA010000373          404 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.387, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000373 DARDHA010000000
VERSION     DARDHA010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.387"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>404
                     /locus_tag="SDA10_002993"
     CDS             <1..>404
                     /locus_tag="SDA10_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241807.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DARDHA010000374          400 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.388, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000374 DARDHA010000000
VERSION     DARDHA010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.388"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          142 a           58 c           48 g          152 t
ORIGIN      
        1 tgttttgttt gtaattccta attttgctcc acttttagat aatttttagg tctatttaac
       61 tcatcagaca ttctatgctc atttttctcc aaatttgttt caactctttc gtacttaatt
      121 ttgcgtattt gaaaattctt cttttagagt acttttatta cgatactagt atgatatata
      181 tctatagcaa acagttaaaa tcttcctaaa ataagcgaat agcattccta aaacctataa
      241 aatttttaga tgaagcgaca agtggacaat taataaacta cttgaacatg aaattatgga
      301 tcacctttca aatttagaaa atggaattct tatacttatt gcgcaacatt tattgattgc
      361 aaaattatgt gacaaaaaaa actagtagaa gaaagaataa
//
LOCUS       DARDHA010000375          391 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.390, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000375 DARDHA010000000
VERSION     DARDHA010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.390"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..378
                     /locus_tag="SDA10_002994"
     CDS             <1..378
                     /locus_tag="SDA10_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEG4241808.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          150 a           41 c           67 g          133 t
ORIGIN      
        1 ccaatagaat ttgacaatga tgaaagattg ctgctaatat atgcgtttat ggatgaaaat
       61 tctatctttt caactgataa ttcaacttat agaaaagtag ttcaatggga aagtgataac
      121 aatttaagga gttacttgag tgatagagta tttgatgtac tagaaaaatt aagtaaatcg
      181 ggaattttaa aggaagaata cgattcttct tttgatgagt atccatcacg ctatattttg
      241 atttcagagg actataagaa atttaaaagg tattttttaa aaaatgaaaa aaagatatct
      301 gttattatgg agaaatacaa agctccaata caggctgatc cattttccaa tttggatgaa
      361 tcagatttac cgttttaaat tttaaataga t
//
LOCUS       DARDHA010000376          388 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.391, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000376 DARDHA010000000
VERSION     DARDHA010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.391"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          159 a           45 c           48 g          136 t
ORIGIN      
        1 agttgcccaa ctttaaaggt tattcttata atattttact tcattttcca atagaaaatt
       61 caaaggatta ctggtttttc taaaatagtt tagtttaaat acataagtat cctccgacac
      121 tgtattagtt cataatcatc taaatacaaa attttcaaat aaaaatattt caagctctat
      181 cgttgacgaa tgtaaacgat acttataaag taatagaaat tgattacatt tgtaaactta
      241 aaatggttaa tttatctcac ataaaactaa gaataaaata atgattaaaa gtggttagtt
      301 tttatacgat ttagcctatc ggtaaaaatg aattagagag tgtaaaatat tttgtgtaaa
      361 tgaaaaaatc catacaaaaa aggaagtc
//
LOCUS       DARDHA010000377          385 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.392, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000377 DARDHA010000000
VERSION     DARDHA010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.392"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(63..263)
                     /locus_tag="SDA10_002995"
     CDS             complement(63..263)
                     /locus_tag="SDA10_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG4241809.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          118 a           73 c           59 g          135 t
ORIGIN      
        1 gaattttttc gtacaaaaaa accgtctttt ctttcgaaaa gacggttaaa agtcaatttt
       61 tattaagctt taacaatgtt tgttgcttga gggccacgtt gaccatcttc aacatcgtaa
      121 gacactgctt ggccttcttc taaagttttg aagccgtcac cttggatagc tgaaaaatgt
      181 gcaaatacat catttccatc ttcaccagtg ataaagccaa aacctttgtc tgcgttgaac
      241 cattttactg taccgttatt cataaataaa ttcctcctga tacgtatcaa tgtacgtttt
      301 ttcgttgcaa taaatatttg ataggcaaaa ggagttttat ctatgtagat atatcgctca
      361 aattacgttt caaaatagat tactc
//
LOCUS       DARDHA010000378          380 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.denovo.393, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000378 DARDHA010000000
VERSION     DARDHA010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.denovo.393"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          143 a           45 c           80 g          112 t
ORIGIN      
        1 cattggtcgt tgaatgggct gaatagtagt gtataaaaca attttgtgga gcgtgaagct
       61 tttatggtag aggaaaatac gaataaagtg atgttgataa aaagattcct atcttgaata
      121 tgaatcgaga tgtcttgaaa atgaatgagt ttgaccaatc attatcctat cgaaatttac
      181 agtttcgttc gattggtctg tcaattaatg aaaaagtaaa aaagaatatg ttctaggaag
      241 agggcggcac atctaaattt ttaagtaacg gacagttcaa atatcaagca ttgaaagagg
      301 ctatcctact gtttgaagga atacgaatat ataaaaacga caaagaggaa aaagtagttc
      361 attaaatccc ttcaagtgat
//
LOCUS       DARDHA010000379        13213 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.guided.073, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000379 DARDHA010000000
VERSION     DARDHA010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.guided.073"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(204..2162)
                     /locus_tag="SDA10_002996"
     CDS             complement(204..2162)
                     /locus_tag="SDA10_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG4241810.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="SDA10_002997"
     CDS             complement(2159..3031)
                     /locus_tag="SDA10_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG4241811.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="SDA10_002998"
     CDS             complement(3373..4245)
                     /locus_tag="SDA10_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG4241812.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="SDA10_002999"
     CDS             4373..4942
                     /locus_tag="SDA10_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEG4241813.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="SDA10_003000"
     CDS             5009..5746
                     /locus_tag="SDA10_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG4241814.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="SDA10_003001"
     CDS             5977..7245
                     /locus_tag="SDA10_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEG4241815.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="SDA10_003002"
     CDS             7732..8196
                     /locus_tag="SDA10_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG4241816.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="SDA10_003003"
     CDS             8180..8809
                     /locus_tag="SDA10_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG4241817.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="SDA10_003004"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="SDA10_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEG4241818.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="SDA10_003005"
     CDS             10688..11134
                     /locus_tag="SDA10_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEG4241819.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="SDA10_003006"
     CDS             complement(11333..11848)
                     /locus_tag="SDA10_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG4241820.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="SDA10_003007"
     CDS             11965..13167
                     /locus_tag="SDA10_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG4241821.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DARDHA010000380         8076 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.guided.125, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000380 DARDHA010000000
VERSION     DARDHA010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.guided.125"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(134..577)
                     /locus_tag="SDA10_003008"
     CDS             complement(134..577)
                     /locus_tag="SDA10_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEG4241822.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="SDA10_003009"
     CDS             complement(795..2135)
                     /locus_tag="SDA10_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG4241823.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="SDA10_003010"
     CDS             2404..3300
                     /locus_tag="SDA10_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEG4241824.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="SDA10_003011"
     CDS             complement(3450..4391)
                     /locus_tag="SDA10_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG4241825.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="SDA10_003012"
     CDS             4517..5638
                     /locus_tag="SDA10_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG4241826.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="SDA10_003013"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="SDA10_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEG4241827.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="SDA10_003014"
     CDS             7426..7992
                     /locus_tag="SDA10_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG4241828.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         2675 a         1507 c         1598 g         2296 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttt
//
LOCUS       DARDHA010000381         6205 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.guided.150, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000381 DARDHA010000000
VERSION     DARDHA010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.guided.150"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            165..281
                     /locus_tag="SDA10_003015"
     CDS             165..281
                     /locus_tag="SDA10_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEG4241829.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            297..1100
                     /locus_tag="SDA10_003016"
     CDS             297..1100
                     /locus_tag="SDA10_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG4241830.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            1335..1571
                     /locus_tag="SDA10_003017"
     CDS             1335..1571
                     /locus_tag="SDA10_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241831.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1578..3530
                     /locus_tag="SDA10_003018"
     CDS             1578..3530
                     /locus_tag="SDA10_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEG4241832.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3602..4099)
                     /gene="dfrG"
                     /locus_tag="SDA10_003019"
     CDS             complement(3602..4099)
                     /gene="dfrG"
                     /locus_tag="SDA10_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEG4241833.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            4461..5501
                     /locus_tag="SDA10_003020"
     CDS             4461..5501
                     /locus_tag="SDA10_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG4241834.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            5599..5787
                     /locus_tag="SDA10_003021"
     CDS             5599..5787
                     /locus_tag="SDA10_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEG4241835.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(5845..6198)
                     /locus_tag="SDA10_003022"
     CDS             complement(5845..6198)
                     /locus_tag="SDA10_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG4241836.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
BASE COUNT         2140 a          991 c         1120 g         1954 t
ORIGIN      
        1 gctcttacgt gctattattt aagtgactat ttaaaaggag ttaataaata tgcggcaagg
       61 tattcttaaa taaactgtca atttgatagc gggaacaaat aattagatgt ccttttttag
      121 gagggcttag ttttttgtac ccagtttaag aataccttta tcatgtgatt ctaaagtatc
      181 cagagaatat ctgtatgctt tgtataccta tggttatgca taaaaatccc agtgataaaa
      241 gtatttatca ctgggatttt tatgcccttt tgggtttttg aatggaggaa aatcacatga
      301 aaattattaa tattggagtt ttagctcatg ttgatgcagg aaaaactacc ttaacagaaa
      361 gcttattata taacagtgga gcgattacag aattaggaag cgtggacaaa ggtacaacga
      421 ggacggataa tacgctttta gaacgtcaga gaggaattac aattcagaca ggaataacct
      481 cttttcagtg ggaaaatacg aaggtgaaca tcatagacac gccaggacat atggatttct
      541 tagcagaagt atatcgttca ttatcagttt tagatggggc aattctactg atttctgcaa
      601 aagatggcgt acaagcacaa actcgtatat tatttcatgc acttaggaaa atggggattc
      661 ccacaatctt ttttatcaat aagattgacc aaaatggaat tgatttatca acggtttatc
      721 aggatattaa agagaaactt tctgccgaaa ttgtaatcaa acagaaggta gaactgtatc
      781 ctaatatgtg tgtgacgaac tttaccgaat ctgaacaatg ggatacggta atagagggaa
      841 acgatgacct tttagagaaa tatatgtccg gtaaatcatt agaagcattg gaactcgaac
      901 aagaggaaag cataagattt cagaattgtt ctctgttccc tctttatcat ggaagtgcaa
      961 aaagtaatat agggattgat aaccttatag aagttattac taataaattt tattcatcaa
     1021 cacatcgagg tccgtctgaa ctttgcggaa atgttttcaa aattgaatat acaaaaaaaa
     1081 gacaacgtct tgcatattaa gtagttcagt tttggagtac aaaaagtgaa aatgtaaacc
     1141 taaatgagta tcaaattgca actgaatttg atactcgttt tttattgtta aatcaacgtt
     1201 tgttgttaat ttacaccaca aaatgtaagt taaaatgtat ataaaaatgc gaacgtgaaa
     1261 taattattgt aggaatgcaa attggtgtgt taaaatggtt acaatttact aagcaaaaag
     1321 gggtgcattt tcctatgcaa tttcttgagc caaaaaataa gaacgctaag tctgtggatt
     1381 gggaaatatc agagcaagta agggtgatag ttaagcaata cgctgaatat gcagagcgta
     1441 ctgaaagcga agcagtggat gaattcttat tgaacatttt agacgataag aagtttattg
     1501 aatggattgc caacaagcga agtaataagc ggattgttga aaaaatgggt atcaaagata
     1561 gagtgggttg atagacaatg accaagctta aaagattagc cacgaaggaa gatgaaattg
     1621 tagatgttaa aattccgatt tcaaacgaag aattaaaaga tagggctaaa caatatgatt
     1681 tattaactcc aaaacgattt gcaacaaggt ataacaaaat gttgttcttg ccaacgtctt
     1741 ttaaatggaa cggttctgaa tatcccattc aatataatta ctgcatcaat ccgttttgtt
     1801 gtaattttgg gaaagaacag cacaaattca aagatgtaaa agggaaacca agtcgatata
     1861 aaatgactgg tagttccaaa gataaaggtc ataaaggtat gtactgcaat gacaatccta
     1921 ttggcagagg tgtatcgcaa aactgtacag taaccccttt atctaattgg tctgttgttg
     1981 aagaaattaa acgccttata gaaatcaata gcatccaaga tgttgagcca gattatcaat
     2041 ttcataaaga aggctgctcg gaagaagaat caaccccatt caatgagccg aagcaatttt
     2101 acaaaagggg gaaaagtagg ggcaaatctc aacgctatca atgtaaggcg tgtaagaagt
     2161 tcactaatgt tctaccaaaa agagaagaaa ccactactta tcaccaacaa aagaacacca
     2221 ttcttccaat gttcgctaag atggttgttg gaagggtttc agtcagtcgt acttgtgata
     2281 ttttaggaat tggagttgga acctactatc ataagctgga gtggctgtat aggcgttgtt
     2341 tggagttttt agaacgatat gagacacaac cccttcaaac aaagaaattt aacgagatgt
     2401 ggcttaatac ggacaagatg cactactacc taaataacgt ccgtaagaaa ggacaaggtt
     2461 caaagaaata tacggggttt gaagacctta atatgcaaac gtatatagtt gtatctgctg
     2521 aggtcttatc aagatatgtg ttccgttctg atgttgccta tgattggaat atttcaatgg
     2581 atgaattaaa tgaagataca agaaaattca aagaagacca tctcaatact tttagtcgta
     2641 aaaatgatag gttagattgg tcttattatc cacaagaacc atccgctaac gatagcgaaa
     2701 ataggaatgc ttatcttcac gaattaggga aaattactaa taggagtaga tttgtcgatg
     2761 gtttaaatgt tgatgctcct tatactacaa ctgctcacta ttggttaatt aaacaaatgg
     2821 ttaatgccga cgaatggcga atgattagtg atgatgactt ctctattaga aacgcctttt
     2881 atagagtatt cacgaaagaa ttaaggttga gcgatgcaca tcattttatt tgccaagtta
     2941 acaagacgaa aagcagaaaa caatgcttaa aagaattcgg acaagcaaaa gcagaactgc
     3001 tcgattgggg agatatcaga ggattcaaaa caaagtttct acggactttg gcatctcatt
     3061 atctaactga attactaaca agtcatcaat tccacgaaga ggctattagt aaagatggag
     3121 aaagatatcg caaatatgcc gataacccta tcaaacatcc attagccact aaggataaag
     3181 gattttattc agttgattgt agaacggatt tatctgctct ggaaccaaac gaaatcgcaa
     3241 agatgctatt aaatgttaat gaccattcga ccaatagctt tattcaacag ataagacgtt
     3301 atatatcatc tcttgaaaga ccattaacaa cagctcgtgg tgataagaag agctatattt
     3361 acgctaactt caatccgaag tatgctcaat ttgcgattac aattctaaga acttactaca
     3421 acttttgtcg cccttttaaa tcagcggata aaaaggtttt gacacctgct caaagattag
     3481 ggattacaga taaacaattt gattggaaag atataattta tttcaaatga caaaaagagc
     3541 ttggagtaat cccaagctct tgtgctttat ctttactgtt cttcttcaat taacgcaccc
     3601 gttaactcaa taagtttttt ctttcatata catgaaaata gtagttatac ggatttttat
     3661 cattctttat ccctttttgg gcaaatacct cattccattc ctcataattc acttctggaa
     3721 aaaaagtatc tccttcgaat tcatgatgta tttttgtgat gtacattttc tcaacataag
     3781 ggaaaaacaa attataaatc tgttctcctc cgaaaataaa aatttcttct tcgtttttac
     3841 ataactcaaa aacatcttct attgaatgaa caatttcaca accattaaag gtaaacccct
     3901 tatctctcgt cagaataata tttcttctgt caggtaaggc tcttccgatt gattcaaggt
     3961 tcttcctacc taatattatc ggatgtccct ttgtagtatt tttaacatat tcccagtcct
     4021 tgggaatcct ccaaggaatg tcattctctt tgccaatcac tctattctta tccatcgcag
     4081 caatcaaaga aactttcatt tataacacag cctttttaac taatttacca tctaatttct
     4141 tatttctatc aaaaaacctt ttctcattat cctgcccgtt agttgaataa gcaatttttt
     4201 ctataagtta tattatcata ataccacaaa atcacaaatt ttcattcaat tatcattttc
     4261 aattgcattt tataaatcat tttgtatgta atttggtatg taagttaaaa tgaaaaaagt
     4321 agtccatttt gaagtacatt ttgtcatttt gtactccaaa actgaactac ttacttgcat
     4381 atatacgcct ttatagtgga gtactacatt tacgagattc ggttagagta tcagaaaaag
     4441 aaaaaataaa agttacagaa atgtatactt caataaatgg tgaattatgt aagattgata
     4501 gagcttattc tggagaaatt gttattttgc aaaatgagtt tttgaagtta aatagtgttc
     4561 ttggagatac aaaactattg ccacagagaa aaaagattga aaatccgcac cctctactac
     4621 aaacaactgt tgaaccgagt aaacctgaac agagagaaat gttgcttgat gcccttttgg
     4681 aaatctcaga tagtgatccg cttctacgat attacgtgga ttctacgaca catgaaatta
     4741 tactttcttt cttagggaaa gtacaaatgg aagtgattag tgcactgttg caagaaaagt
     4801 atcatgtgga gatagaacta aaagagccta cagtcattta tatggagaga ccgttaaaaa
     4861 atgcagaata taccattcac atcgaagtgc cgccaaatcc tttctgggct tccattggtt
     4921 tatctgtatc accgcttccg ttgggaagtg gaatgcagta tgagagctcg gtttctcttg
     4981 gatacttaaa tcaatcattt caaaatgcag ttatggaagg gatacgctat ggttgcgaac
     5041 aaggattata tggttggaat gtgacggatt gtaaaatctg ttttaagtac ggtttatact
     5101 atagccctgt tagtactcca gcagattttc ggatgcttac tcctattgta ctggagcaag
     5161 cctttagaaa agctggaaca gaattgttag agccatatct tagttttaaa gtttatgcac
     5221 cacaggaata tctttcacgg gcatataacg atgctcccaa atattgtgca aatatcgtaa
     5281 atactcaact gaaaaataat gaggtcatta ttattggaga aattcctgct cgatgtattc
     5341 aagattatcg caatgattta acttttttta caaatgggct tagtgtttgt ttagcagagc
     5401 taaaaggata tcaggttacc actggcgaac ctgtttgcca gacccgtcgt ctaaatagtc
     5461 ggatagataa agtaagatat atgttcaata aaataactta gtgcgtttta tgttgttata
     5521 taaatatggt ttcttattaa ataagatgaa atattcttta atatagattt gaattaaagt
     5581 ggaaaggagg agattgttat tataaactac aagtggatat tgtgtcctat ttgtggaaat
     5641 aaaacaagac tacgaatacg agtggatact atacttaaaa atttcccttt atactgcccc
     5701 aaatgtaaga acgaaacttt aattaatgtt caaaaaatga atataataac aatcaaagag
     5761 ccagacgcca agacgcagag ccgataattt gagaaatgaa actctcatct tatcggctct
     5821 ttttgtttat ctgaatttta ctgactagcc ttcaatattt cttgcttcat tgataccttt
     5881 tgctagtgct tccattaagg atagttcttt gtctgtaaag ctatccatgt atttttctac
     5941 ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt aagaaaaatt catcaataga
     6001 tacatggagt aatgatacaa gatcatacag cacttgtgta cttgggtgtt gtcctttgtt
     6061 ttctatattt gttaaataac gtgggtcaat ttcaatcatt gttcccactt gttcacgagt
     6121 gagaccccgt ttcattcgag cctctttaat cgctaaacca acggctctaa aatcgtattt
     6181 atcttctttt ttacgcatac taaat
//
LOCUS       DARDHA010000382         5613 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.guided.162, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000382 DARDHA010000000
VERSION     DARDHA010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.guided.162"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(40..1026)
                     /locus_tag="SDA10_003023"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="SDA10_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR"
                     /locus_tag="SDA10_003024"
     CDS             1178..1873
                     /gene="vanR"
                     /locus_tag="SDA10_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEG4241837.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="SDA10_003025"
     CDS             1851..3005
                     /locus_tag="SDA10_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEG4241838.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3220..4188
                     /locus_tag="SDA10_003026"
     CDS             3220..4188
                     /locus_tag="SDA10_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEG4241839.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4181..5212
                     /gene="vanA"
                     /locus_tag="SDA10_003027"
     CDS             4181..5212
                     /gene="vanA"
                     /locus_tag="SDA10_003027"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEG4241840.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5218..5583
                     /locus_tag="SDA10_003028"
                     /pseudo
     CDS             5218..5583
                     /locus_tag="SDA10_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
BASE COUNT         1697 a         1091 c         1262 g         1563 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt
     3181 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
     3241 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
     3301 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
     3361 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
     3421 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
     3481 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
     3541 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
     3601 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
     3661 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
     3721 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
     3781 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
     3841 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
     3901 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
     3961 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
     4021 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
     4081 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
     4141 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     4201 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     4261 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     4321 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     4381 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     4441 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     4501 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     4561 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     4621 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     4681 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     4741 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     4801 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt
     4861 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     4921 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     4981 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     5041 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     5101 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     5161 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     5221 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     5281 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     5341 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     5401 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa
     5461 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
     5521 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcggttct
     5581 gttgcaaagt tttaaataaa gaataaaatc cct
//
LOCUS       DARDHA010000383         2850 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.guided.221, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000383 DARDHA010000000
VERSION     DARDHA010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.guided.221"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1434
                     /locus_tag="SDA10_003029"
     CDS             <1..1434
                     /locus_tag="SDA10_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG4241841.1"
                     /translation="PVKANSYYVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDD
                     KVNPTQYEYTINFKGFRNTYFKQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMK
                     FKQVQRVHQLTRVEIEAYLSELNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKI
                     LIYSEDYPKIPRAKPRFIDEFVLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLK
                     KGCLLEDKDGDFFLKYYQWKMKKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRK
                     DGSPLKQETFRGELNKLAYEQNIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQK
                     FLGHESPEMTSRYAHIFDETLKNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKK
                     NINAQVLPNGYCRLPVVAGGCPHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAK
                     DKQWQRQVETNSRVKERLEQIIGSLTG"
     gene            1441..1818
                     /locus_tag="SDA10_003030"
     CDS             1441..1818
                     /locus_tag="SDA10_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG4241842.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            1972..2754
                     /locus_tag="SDA10_003031"
     CDS             1972..2754
                     /locus_tag="SDA10_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HEG4241843.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
BASE COUNT         1059 a          402 c          572 g          817 t
ORIGIN      
        1 cctgtaaaag ctaattccta ctatgttact aatctaaaac aatttatgga gttttatgag
       61 aacttttatt ttgatggaga ggagtgggat aaagacgttt gggatagacg taacttacct
      121 ttgccagatg ataaggttaa cccaacacaa tatgaatata caattaactt taaagggttt
      181 cggaatacat attttaaaca acttgtaaaa agatattgta agttgagatt gaacatggat
      241 agcttttcct atgtaagtga tattgcccaa agacttaaag agttctttaa ttttctggac
      301 atgaaattta aacaagttca gagagtacac caattaacga gagtggaaat tgaagcatat
      361 ttaagtgaac taaacatgat gggaataaaa cctagtacaa taactgggag gatctctata
      421 ttggaaggac tatttagtac ccttcttagg ctagaatggg atgatgttcc ttccaaaata
      481 ttaatttatt ctgaggacta tccgaaaata ccaagagcaa aaccacgctt tatagatgaa
      541 ttcgtcctag agcaattgaa cagtcatctt gataaattac ccgaatatat agctacgatg
      601 actatgattg ttcaagaatg tggaatgagg ataagtgaat tgtgcacctt gaaaaaaggc
      661 tgtctattag aggacaaaga tggagatttc tttttaaagt attatcaatg gaaaatgaaa
      721 aaggagcata tagttccaat atctaaagag gtagctttac ttattaaagt tcgggaagat
      781 aaagtttcag aggaatttcc agatagtgaa tacctctttc caagaaaaga tggatcgcca
      841 ttaaaacaag aaacatttag aggtgagtta aataaattag cttatgagca aaatatagtg
      901 gataaatcag gtgagattta tagattccat gcccatgcct ttcgccatac agtaggaaca
      961 agaatgatta acaacgggat gccccagcat attgtgcaga aatttttggg gcatgaaagc
     1021 ccagaaatga caagcagata cgctcatatc tttgatgaaa ctctaaaaaa tgaatttact
     1081 aaatttcagg aaaaactggt taccaataat ggagatgtgc ttgatctaga tgaagataat
     1141 gaagtcgatg atgtagagct tcaatggttc aagaaaaata taaatgcaca agtgcttcca
     1201 aatggttatt gtagattgcc agtagtagca ggtggttgtc cacatgcgaa tgcatgctta
     1261 gattgcactc acttctgtac cagtaagcaa ttcttaccac agcacgaaga acagttagag
     1321 cgtacagaag agttattagc catagctaag gataaacaat ggcaaagaca agtagagact
     1381 aatagccgtg ttaaagagcg tttagaacaa atcattggaa gtttgacggg gtaattatca
     1441 atggataaac aagttagaaa tacaacagaa attgtacgtt tggcgaagca gaaatcaaaa
     1501 aagacaaggg aaaaagtaga caaagcgatt tctaaatttt cgattgaagg taaagttatt
     1561 aattttaatt caatagcaaa ggaagctaat gtttctaaat catggcttta taaggaacac
     1621 gatattaggc aaagaatcga atcccttcgt gagcgtcaaa taacagcaaa tgtagtctca
     1681 aaacccaaga aaagttctcg ttcggaggaa atccttatta aaaccttaaa aagaagagta
     1741 atggaattag aaaaagaaaa taaaaaatta cagaaccaaa ttcaaaaatt atatggagat
     1801 ctgtataata aagaataatt attaatctgt agacaaattg tgaaaggatg tacttaaacg
     1861 ctaacggtca gctttattga acagtcattt aggtatatgt ccaatctagg gtaagtaaat
     1921 tgagtatcaa tataaacttt atatgaacat aatcagttac gaggtgaaat catgagcaat
     1981 ttgattaacg gaaaaatacc aaatcaagcg attcaaacat taaaaatcgt aaaagattta
     2041 tttggaagtt caatagttgg agtatatcta tttggttcag cagtaaatgg tggtttacgc
     2101 attaacagcg atgtagatgt tctagtcgtc gtgaatcata gtttacctca attaactcga
     2161 aaaaaactaa cagaaagact aatgactata tcaggaaaga ttggaaatac ggattctgtt
     2221 agaccacttg aagttacggt tataaatagg agtgaagttg tcccttggca atatcctcca
     2281 aaaagagaat ttatatacgg tgagtggctc aggggtgaat ttgagaatgg acaaattcag
     2341 gaaccaagct atgatcctga tttggctatt gttttagcac aagcaagaaa gaatagtatt
     2401 tctctatttg gtcctgattc ttcaagtata cttgtctccg tacctttgac agatattcga
     2461 agagcaatta aggattcttt gccagaacta attgagggga taaaaggtga tgagcgtaat
     2521 gtaattttaa ccctagctcg aatgtggcaa acagtgacta ctggtgaaat tacctcgaaa
     2581 gatgtcgctg cagaatgggc tatacctctt ttacctaaag agcatgtaac tttactggat
     2641 atagctagaa aaggctatcg gggagagtgt gatgataagt gggaaggact atattcaaag
     2701 gtgaaagcac tcgttaagta tatgaaaaat tctatagaaa cttctctcaa ttaggctaat
     2761 tttattgcaa taacaggtgc ttacttttaa aactactgat ttattgataa atattgaaca
     2821 atttttggga agaataaagc gtcctcttgt
//
LOCUS       DARDHA010000384         2011 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.guided.249, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000384 DARDHA010000000
VERSION     DARDHA010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.guided.249"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(685..975)
                     /locus_tag="SDA10_003032"
     CDS             complement(685..975)
                     /locus_tag="SDA10_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG4241844.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1183..1875)
                     /locus_tag="SDA10_003033"
     CDS             complement(1183..1875)
                     /locus_tag="SDA10_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG4241845.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          557 a          381 c          303 g          770 t
ORIGIN      
        1 gagtataaca tagctttaag cattacttaa agataactta aaagttatct tttaaatact
       61 taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca aaaaaatttt
      121 cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta atgcagaatt
      181 cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac gaacaagata
      241 tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca ggacgttctc
      301 gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac ctcttcgagt
      361 tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc tgagactagt
      421 aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact aatagggctt
      481 cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa agtttagtga
      541 ttcatcacaa atacttaaat acatgtagtt atatatagat tgaggttctt gtgagcgtat
      601 gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt ccaaaacaag cggctaggac
      661 atttttgtct cagccgcttg ttttctagtt tattctttca tcttgtcgga ccttttttct
      721 tgggtataga cttttctcat attctaccac aattttgggt ttccctaaca catgatagga
      781 tctcacaaca aattgctttc tattattata ataacctgta acgactaact tcatcccttc
      841 gtctacttca gctaggaagt tcaaactatg ggaagcaatc aaacaattga ccccatttaa
      901 actgaaacgg atcaacggtc tggcactaac ttgcaaagta cggatcttat ctactgttcc
      961 atattttttc tccataacct tcacctcaat accattatac gaacatacat tccctttgta
     1021 aagaagaaac tctgttttat ctttttgact ttttgtctat tacctttctt aaaatcatat
     1081 tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa agaggcagca gtatagatta
     1141 ctgttgcttc tttactaatt taggtttact tgttaagaaa gattatttac gcaaatgttc
     1201 ttcaacaatt tgctgagatt tatcagctgt atcttccact tcatcttttg ctttttctaa
     1261 cttttgaata tattgtttca agcttgagtt ttcagcagtt gcttcgtcta atttcttttg
     1321 tgtatctgtt aactgagttt ctacatttgt tttttcttgt gtcagtgttg cgatttgttg
     1381 attcaaacca tttacagtat tttgtaattc tgaaagtttt gcttgtgctt cttgtagtct
     1441 tgctgtccaa tctgcattca gatgattgat ttcatcattt ttttggttga tctcatctag
     1501 tttgacattg atttcatttt gtttgctttg tagttgtgca tccttgctga ctaattgact
     1561 ttctaagttt tgtttttctg ctgccgattg atttttttgg ttttctaatt catttacttt
     1621 tgtttggtat tcttggacat ctgcctttga atcttgcaat gtctgttctt tttgtgctag
     1681 tcgagcggta agatctgtaa gcgcttgctc tttatctgtc aatgtatgat caagctgtgt
     1741 cagattctca ttgattgtat caatatctgt ctctccaccc cacaaaataa tttcatttgc
     1801 gtaccgcgtg cctgtgtagc caattagaca tagaaatgca acgacaatcc caccagtaat
     1861 aaatttcttt ttcatttgca tagctcctta atccaatact tttacatgct aaaagtaagt
     1921 gatctcaaac acaatgtcaa gcgtttttaa attacatgtg taattttaat ataaatacta
     1981 tcaaatactc atttaatcct ccttttttat c
//
LOCUS       DARDHA010000385         1504 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.guided.271, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000385 DARDHA010000000
VERSION     DARDHA010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.guided.271"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            262..345
                     /locus_tag="SDA10_003034"
                     /pseudo
     CDS             262..345
                     /locus_tag="SDA10_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="SDA10_003035"
     CDS             394..477
                     /locus_tag="SDA10_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HEG4241846.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="SDA10_003036"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="SDA10_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEG4241847.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DARDHA010000386         1350 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.guided.291, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000386 DARDHA010000000
VERSION     DARDHA010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.guided.291"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            47..298
                     /locus_tag="SDA10_003037"
                     /pseudo
     CDS             47..298
                     /locus_tag="SDA10_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            391..1185
                     /locus_tag="SDA10_003038"
                     /note="aph(3')-IIIa"
     CDS             391..1185
                     /locus_tag="SDA10_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEG4241848.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          425 a          234 c          322 g          369 t
ORIGIN      
        1 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
       61 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      121 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      181 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      241 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      301 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      361 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      421 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      481 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      541 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      601 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      661 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      721 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      781 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      841 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      901 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
      961 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1021 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1081 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1141 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1201 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1261 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1321 gcgcctacga ggaatttgta tcgataagaa
//
LOCUS       DARDHA010000387         1297 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.guided.294, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000387 DARDHA010000000
VERSION     DARDHA010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.guided.294"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..1272
                     /gene="aph(2'')-I"
                     /locus_tag="SDA10_003039"
                     /pseudo
     CDS             1..1272
                     /gene="aph(2'')-I"
                     /locus_tag="SDA10_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
BASE COUNT          534 a          104 c          222 g          437 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaaccg aatcattaaa aaaacattat
       61 acagagcctt gggaagatga agtttttaga gtaattattg aatataacaa tgttcctatt
      121 ggatatggac aaatatataa aatgtatgat gagttatata ctgattatca ttatccaaaa
      181 actgatgaga tagtctatgg tatggatcaa tttataggag agccaaatta ttggagtaaa
      241 ggaattggta caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct
      301 aatgcagtta ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa
      361 tctggtttta gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa
      421 gattgttatt taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa
      481 tatttaattg agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt
      541 ggttatgata gtgtggcata tttagttaat aatgaataca tttttaaaac aaaatttagt
      601 actaataaga aaaaaggtta tgcaaaagaa aaagcaatat ataatttttt aaatacaaat
      661 ttagaaacta atgtaaaaat tcctaatatt gaatattcgt atattagtga tgaattatct
      721 atactaggtt ataaagaaat taaaggaact tttttaacac cagaaattta ttctactatg
      781 tcagaagaag aacaaaattt gttaaaacga gatattgcca gttttttaag acaaatgcac
      841 ggtttagatt atacagatat tagtgaatgt actattgata ataaacaaaa tgtattagaa
      901 gagtatatat tgttgcgtga aactatttat aatgatttaa ctgatataga aaaagattat
      961 atagaaagtt ttatggaaag actaaatgca acaacagttt ttgagggtaa aaagtgttta
     1021 tgccataatg attttagttg taatcatcta ttgttagatg gcaataatag attaactgga
     1081 ataattgatt ttggagattc tggaattata gatgaatatt gtgattttat atacttactt
     1141 gaagatagtg aagaagaaat aggaacaaat tttggagaag atatattaag aatgtatgga
     1201 aatatagata ttgagaaagc aaaagaatat caagatatag ttgaagaata ttatcctatt
     1261 gaaactattg tttattgatg ataatatggt ttttgat
//
LOCUS       DARDHA010000388         1021 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.guided.313, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000388 DARDHA010000000
VERSION     DARDHA010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.guided.313"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            306..854
                     /gene="aac(6')"
                     /locus_tag="SDA10_003040"
     CDS             306..854
                     /gene="aac(6')"
                     /locus_tag="SDA10_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEG4241849.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          364 a          146 c          209 g          302 t
ORIGIN      
        1 taaaaattct aaaaattaag attattatat gaaaacaggt tttatttgtc aattcataat
       61 tggttttttt taaagcaaaa gataatctat atcatgatga acaaacagag ttttttcctc
      121 caattgatgg ctttcctgat ctttcctatt ttgaataaag tgtttctgta taattttttt
      181 attttataag taagataaat aatgaaagaa tagagatact gattggtagc taaagatttt
      241 tgagcgagaa catttcgttt tttcaaaagg actgttagaa taagcaagaa caagggggaa
      301 gacgtatgat aatcagtgaa tttgaccgta ataatccagt attgaaagat cagctttctg
      361 atttactgag actgacttgg ccggaagaat atggagacag ctcggcagaa gaagtagaag
      421 aaatgatgaa tccagaacga atcgcggtag cagcggtaga ccaagatgag ttagtaggat
      481 ttattggtgc aatccctcaa tacggtatca caggttggga attgcatcca ttagttgtag
      541 aaagctcccg acgaaagaac caaataggta ctcgattagt caattactta gaaaaagaag
      601 tagcttccag aggaggaatc acgatttatt taggtacgga tgatttagac catggaacaa
      661 cgttaagtca aacggacctg tatgagcata catttgataa agtggcttcc atccagaacc
      721 ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta taaaatcgta ggtgtcttac
      781 caaatgcaaa tggctgggac aaaccggata tttggatggc aaaaacgatt attcctcgac
      841 caaattctca ataataaaaa gaggttgtga cagaagtaat cagcttcaag aaataagaag
      901 gaatttacga aaattacttc ttgaattttt gtgaaattcc aacttatttc cgaagaagct
      961 acttttggaa caccgtgtac cagaggttgt gaaaaagtcg cttagtttcg agtagtaaga
     1021 a
//
LOCUS       DARDHA010000389          964 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.guided.320, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000389 DARDHA010000000
VERSION     DARDHA010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.guided.320"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            55..114
                     /locus_tag="SDA10_003041"
                     /pseudo
     CDS             55..114
                     /locus_tag="SDA10_003041"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
     gene            172..903
                     /gene="erm(A)"
                     /locus_tag="SDA10_003042"
     CDS             172..903
                     /gene="erm(A)"
                     /locus_tag="SDA10_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HEG4241850.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
BASE COUNT          372 a          146 c          155 g          291 t
ORIGIN      
        1 gtagaaatta ctttatctca ttcataatga aaaaaatgga aaggagataa aagtatgggt
       61 actttttcta tatttgttat taataaagtt cgttatcaac caaatcaaaa ttaattggtt
      121 ataatgaacg cttaatgtca gttcattata accagtaagg agaaggttat aatgaaccag
      181 aaaaacccta aagacacgca aaattttatt acttctaaaa agcatgtaaa agaaatattg
      241 aatcacacga atatcagtaa acaagacaac gtaatagaaa tcggatcagg aaaaggacat
      301 tttaccaaag agctagtcaa aatgagtcga tcagttactg ctatagaaat tgatggaggc
      361 ttatgtcaag tgactaaaga agcggtaaac ccctctgaga atataaaagt gattcaaacg
      421 gatattctaa aattttcctt cccaaaacat ataaactata agatatatgg taatattcct
      481 tataacatca gtacggatat tgtcaaaaga attacctttg aaagtcaggc taaatatagc
      541 tatcttatcg ttgagaaggg atttgcgaaa agattgcaaa atctgcaacg agctttgggt
      601 ttactattaa tggtggagat ggatataaaa atgctcaaaa aagtaccacc actatatttt
      661 catcctaagc caagtgtaga ctctgtattg attgttcttg aacgacatca accattgatt
      721 tcaaagaagg actacaaaaa gtatcgatct tttgtttata agtgggtaaa ccgtgaatat
      781 cgtgttcttt tcactaaaaa ccaattccga caggctttga agcatgcaaa tgtcactaat
      841 attaataaac tatcgaagga acaatttctt tctattttca atagttacaa attgtttcac
      901 taaattaaag taataaagcg ttctctaatt tcacaagagg acgctttatt cttcccaaaa
      961 attg
//
LOCUS       DARDHA010000390          962 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE3791
            SAMEA114569765-rid20120273.guided.321, whole genome shotgun
            sequence.
ACCESSION   DARDHA010000390 DARDHA010000000
VERSION     DARDHA010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569765
            Sequence Read Archive: ERR12203589
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001973530.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001973530.1
            Genome Coverage       :: 47x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/08/2023 00:27:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,987
            Genes (coding)                    :: 2,914
            CDSs (with protein)               :: 2,914
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 23 of 73
            Pseudo Genes (incomplete)         :: 38 of 73
            Pseudo Genes (internal stop)      :: 19 of 73
            Pseudo Genes (multiple problems)  :: 6 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569765-rid20120273.guided.321"
                     /isolate="VRE3791"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            27..>962
                     /gene="vanY"
                     /locus_tag="SDA10_003043"
     CDS             27..>962
                     /gene="vanY"
                     /locus_tag="SDA10_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HEG4241851.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          347 a          131 c          194 g          290 t
ORIGIN      
        1 ctatatagtt aaggaggaga ctgaaaatga agaagttgtt ttttttattg ttattgttat
       61 tcttaatata cttaggttat gactacgtta atgaagcact gttttctcag gaaaaagtcg
      121 aatttcaaaa ttatgatcaa aatcccaaag aacatttaga aaatagtggg acttctgaaa
      181 atacccaaga gaaaacaatt acagaagaac aggtttatca aggaaatctg ctattaatca
      241 atagtaaata tcctgttcgc caagaaagtg tgaagtcaga tatcgtgaat ttatctaaac
      301 atgacgaatt aataaatgga tacgggttgc ttgatagtaa tatttatatg tcaaaagaaa
      361 tagcacaaaa attttcagag atggtcaatg atgctgtaaa gggtggcgtt agtcatttta
      421 ttattaatag tggctatcga gactttgatg agcaaagtgt gctttaccaa gaaatggggg
      481 ctgagtatgc cttaccagca ggttatagtg agcataattc aggtttatca ctagatgtag
      541 gatcaagctt gacgaaaatg gaacgagccc ctgaaggaaa gtggatagaa gaaaatgctt
      601 ggaaatacgg gttcatttta cgttatccag aggacaaaac agagttaaca ggaattcaat
      661 atgaaccatg gcatattcgc tatgttggtt taccacatag tgcgattatg aaagaaaaga
      721 atttcgttct cgaggaatat atggattacc taaaagaaga aaaaaccatt tctgttagtg
      781 taaatgggga aaaatatgag atcttttatt atcctgttac taaaaatacc accattcatg
      841 tgccgactaa tcttcgttat gagatatcag gaaacaatat agacggtgta attgtgacag
      901 tgtttcccgg atcaacacat actaattggt tctgttgcaa agttttaaat ctactatcaa
      961 at
//
